LOCUS       DAOSHR010000001        35980 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_928_length_35980_cov_5.889882, whole genome shotgun sequence.
ACCESSION   DAOSHR010000001 DAOSHR010000000
VERSION     DAOSHR010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 35980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35980
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_928_length_35980_cov_5.889882"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /locus_tag="PLU28_00005"
     CDS             complement(<1..652)
                     /locus_tag="PLU28_00005"
                     /inference="COORDINATES: protein motif:HMM:NF017946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HOG33474.1"
                     /translation="MLKNKLEKYFSIIKINFSKVLVYKYSFLIEIISLLFQFTASTYI
                     WKYILENSPQIKNYSISSLTNYLLFSTIIKSITQNTTSMDIEKDIRTGNIYNFLILPI
                     DYIYLYLFRDIGLSLGRIFLNSWLIIIYGVVFFDINICFNILNYSLFFISIIFSIIIY
                     SEISILIGLLSFWTISQNGLRYANSVISAFFSGSLIPLDFYPDILKNISLFLPYRSI"
     gene            complement(658..1695)
                     /locus_tag="PLU28_00010"
     CDS             complement(658..1695)
                     /locus_tag="PLU28_00010"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG33475.1"
                     /translation="MTDDIISATNLSKTYKVYLNKKGIIGNIKNIFIRREKINIAAVD
                     NISFIIKSNSVTGLLGPNGAGKTTIIKMLTGILSPTDGTIKSMGLNPFSDRKKYVKNI
                     GVCLGQKPQLWWDLPVIESYKMLSKIYSLEEDSFNKYLNYLIKKWNLEDLLFKPVRKL
                     SLGQLSRCNLIASILHKPKLLFLDEPTLGLDIYYKNIFLEMIKEIKKDLNTTIILTSH
                     NLYEIEELCNNIILIDKGKIIFSDTLQTLKNMYEKENEIILILSKPANDFDGNQLKEK
                     YNLFNIEKLDNNSKIKISFDKKKINLENLISDLLVLFKNNIKDIVFSNSSLEKIIQNI
                     LIKDHSNNIDR"
     gene            complement(1816..3576)
                     /locus_tag="PLU28_00015"
     CDS             complement(1816..3576)
                     /locus_tag="PLU28_00015"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG33476.1"
                     /translation="MKNRKYNHEIIKKSFNIGFQIMKKEFVLMLAVSIAYYSLPLLGI
                     MLNVKIFNMVQVLPGNTQKIPKFVVLVIWYGVYLLFTKFHLIYYTRYFLQYGPLLKFE
                     RLARLMLHNKCDEINAVNYENPELYNYIIQARTASTNVYRIIEINIYLVGILFGSIGV
                     LIYMKDFNPALIIFVIISNIAVLLEKIYSARQYGKTREKTMPLQLEEMAYKESLTSLN
                     FNKEVRILSIEAFLKNKWLGPFEKRVAAEWGADKKVISVNAIAELLKFVGSFGAYAYS
                     AYLLITGKIRLGEFTASLFAFTSLSEYTQEIFELYGYLDQFTTYVKPFFRFMEIFPDK
                     RNERCDCGFTSLRLENVSFKYPGSEKNALEDITLEVKKGEKTAIVGKNGAGKSTLVNM
                     ILGLYDPIKGRVLYNGKEAKAIEKKDLFAGKSAVFQNVNKYKMTVAENVQLGNPQKKD
                     LQEQMKALKESGFPLNRYSMDEKLGKEFGGIDISGGEWQRLSMARGIYKNGSFVVLDE
                     PTSAIDPLQEAELYKKFVQLFNDKTVFLVTHRLGAVSIADKIIVLDDGKIKEHGTHEE
                     LIRKNGYYKKLWDSQVKWYQ"
     gene            complement(3557..5323)
                     /locus_tag="PLU28_00020"
     CDS             complement(3557..5323)
                     /locus_tag="PLU28_00020"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG33477.1"
                     /translation="MNRRKTGYFLSHNLYIKLIFLLKLVLSLLVSVLVVAGITYKKVF
                     IDAVINSGSERNMELIFFYLFLIALVYLIPGMVNFISFWMDKIMKKLLSCELNKCILD
                     KTIKIKYPVLEDQKTQDLISRLDGSHEKIISTMNIITSLVFSAVQFFGVIYMLKQVNL
                     FIMLSIVVFIFLGVFLNIKSGKALYGFWQKYIKNARRYNYLSEILTERSYVLDKSIFG
                     YTEYINVEYNKEFYKAQKENKKAGLKRFKIQSLFETTGIIYMVGCFILLAFPLIGNTI
                     SLGYYVAVTQALGTVFASVNSGFEKIPELTGYRKFRRDFIEFMELAEYEENEGKEISE
                     VEMVEFKNVSFHYPGSKELILDSCSFKIEKGRHYAVVGENGAGKSTIIKLLLGLYEPV
                     EGQILINGVPIKSISSKELSKNISVLFQDFGRFPLTVKENILIGNLSKKSDDNHISQL
                     LNEVNGEDIPSKLSGGINTTLSKKFSGGSDLSGGEWQKVAIGRLLFADKTLKIFDEPT
                     ASLDPIAESEIYEMYNRKLKGKTVIFITHRLGSTKSSDEILLVKNGKIFEKGSHESLM
                     KLNREYYKLYDSQRSLYEKQKI"
     gene            6162..7370
                     /locus_tag="PLU28_00025"
     CDS             6162..7370
                     /locus_tag="PLU28_00025"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-like beta-propeller repeat protein"
                     /protein_id="HOG33478.1"
                     /translation="MKKLLILCTVCMFFLLTSCISIFEEPALELYWHLYFPGKEYKSP
                     DQGTYFTYDETTGYLYVRFSGERVGCFDLAKKKTVWIRYINSRGLTLINLDIELYKNM
                     VYVIRDDVRKKDKKDPVCAICALDKLTGETVWEYAVTDEKFKSGITVHNGFIYIPDYD
                     KRDGKKGMLVKLDAQTGKVVWINKNVQPVGVTKSLIDEKYSLVYVGTFGNDHIGVAPK
                     VYALDIESGETVWERNILWEEPVIVDIVTVPAVYGDSVYVGTWHGEFVRLRRTDGRVV
                     WSAEYPERGFGFGARIWVYKNTLISSFVDGGVVCIDPSDGKILWHTKNPEPTIEDCYM
                     KGNRVYIHGATNSFKCFSADTGKEIWTYTYSNFNGTEYGRMGGAPFKAGKYFITGDVG
                     GNLMIYIEKK"
     gene            7372..7728
                     /locus_tag="PLU28_00030"
     CDS             7372..7728
                     /locus_tag="PLU28_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG33479.1"
                     /translation="MKKLLILCTVCMFFLLTSCIKAPENAFLVIYIMETNCSQKISGA
                     EITIYMQNKKYSSELCETGVYEIDIPVSKKYEPIDIFVCKAGYSNHTTYGLEIEKNKV
                     NLFRVLLHRNEPLKIK"
     gene            8100..9077
                     /locus_tag="PLU28_00035"
     CDS             8100..9077
                     /locus_tag="PLU28_00035"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOG33480.1"
                     /translation="MNIKEIAKLAGVSVATVSRVINDSNDVTEETKEKVRKVISEHNY
                     EPDFIAKSLRGGKTDIFGVIFLKSDSDLNDSYSTAFLMGVLNYMLHHGKRTIVEASYK
                     EDKLKAYKKLISSSLVDGFILLDLEDDDERVKYLKSINIPFVVVGQNSKNDFIFIDTD
                     NTNGGYLGIKSLYSIGCRNILYISGTKGPCVSSQRLDGVKKACGELGISADIQYGDFE
                     KESGYRIAKATQLIGYDGIFCASDFMAYGVMEFMKEKKISLPIIGFDDLSESKILGLT
                     SIRQDIRNVGYLCASKLNDIILGKEASSQILPVECIERSSTKNFRKSQK"
     gene            9104..10315
                     /locus_tag="PLU28_00040"
     CDS             9104..10315
                     /locus_tag="PLU28_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOG33481.1"
                     /translation="MRRVILVLSIIFIVSLAFSSPVKLTMSGWPGNPDEEAAITAGVE
                     AFNAKHSDIQLIWEPIPGDYFSTLKTRLSGGTGPDIFYVDVFVFEELATNNSMLSLDT
                     YIKKDGFKLSDYYNSLVEAFQYKNRTYGIAKDFSTLALYFNKSIFDKCGVAYPTNDET
                     WDSLLIKLQELKKKGVEYPIIVNPELTRMIPFINSFGGSVVDSNMKVSLSQPNSKKGF
                     QFYIDLVTKYKVGYEASAAGAGWEGEAFGKENAAVMMSGPWCLGFLKGSYPNVYNKMG
                     IVEMPKNVKRSTMVYTVCWAINRQSSNKDAAWEAMKFLVTEGQQIFVEKAGVLGSNKN
                     VAAKDTDPIKQVFYKGADYATPWKIPTPTGLFSKANDQINSRIKDLFYNKITLDEALK
                     QINENYDSWIK"
     gene            10383..11522
                     /locus_tag="PLU28_00045"
     CDS             10383..11522
                     /locus_tag="PLU28_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOG33482.1"
                     /translation="MKKRVVQEAMVGYLFAAPIIISICIFTLYPIISAFIYSFTDFNP
                     LEIKKFDIKFNPQDAIEINTGFLPSELSEKEVVIDFDLMTFIELDVGLKLDAEKSDLV
                     QKYFDSSKLLKDFSEGKLDKEIAIKDFMGTYMTMHGDQFRKYRPKFLWFDNFMELLKD
                     QYFWISLGNAFFFSLIVVPIQTLLAVILAVAANSKIKGVKFYKAVFFIPSITSSAAIS
                     MIFWMIYSKPGVINRLFGTSIDWLNEPNTALPAIMLMNIWTTAGYFMVTFLAGLQDIP
                     DSIYEAAEIDGASGLTTFWKITMPLLRPQILFVAITGTIGCMQVFDQIYFLIKNIRNI
                     TISFFIYKNAFEYGKMGYASAVALVLFLIILAITAVQRKYIKDEY"
     gene            11534..12415
                     /locus_tag="PLU28_00050"
     CDS             11534..12415
                     /locus_tag="PLU28_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOG33483.1"
                     /translation="MKVKKSRIIAYTVLIAYVIISLFPFFWALMVSITPLNYYDTITN
                     EQKGIDIMQWPPKINFFKGEVFGAPMTFENYIKIFQVAPLFARWIINTFIYAGLITLG
                     HVLIDTLGGYAFARLKFPFKDLWFMLFLATMMVPGQIIMIPQYTMMVNYGLINTYAGL
                     FLPKLTGVFGLFLLRQFFLNFPKELEEAARIDGAGIIKTYFKVVLPNAKPAVSALAIY
                     SFLGAWNELMWPLLVTSKKEMYTLAMGLNFFKTSYYTFWQYLMAASIIMTVPMIIIFL
                     SFQKEFVETGRSAAVKG"
     gene            complement(12491..14767)
                     /locus_tag="PLU28_00055"
     CDS             complement(12491..14767)
                     /locus_tag="PLU28_00055"
                     /inference="COORDINATES: protein motif:HMM:NF015588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HOG33484.1"
                     /translation="MKDLWEFSVKDYIKEDNYKNGTLFNLANGYMGMRGYNEFSLYNN
                     PGNYIAGIFDRSTAQVTELVNLPNPLLMNIYVNGQKIDLDINNVIDYQRSLDMGNGIL
                     KEAFIVEVQKGLRVQIESERFVSRKNFHRWACRYSVTALDSEALFVFESIIDGGTVNS
                     GHNPKELSAHYKVSSVQDLKPGVMMVAITNDKKIEIAQASCIILKEKKFSRLKFNDLQ
                     CRVQQAGDVIAAKGETIVVYKFGAVYSSRESSNASTDCKREIEAFIADGYEAELEKHS
                     ELWQGFWNSSDIKIQGNEEAQKALRFNIFHLSSCAYDKDPTVSIGAKGLHGEGYKGHV
                     FWDTETFMLPFFIYTQPQVAKALLMYRYNTLDGARRNAFMNGFSGAQYAWESADDGLE
                     TTPKWGYDYNNKPVRIWTGDIEVHISSDIVFSIFEYYRATEDKDFMFKYGSEIIFEVC
                     RFWTGRAVYNKNKDIWEINSVIGPDEFHEHVDNNFYTNYLVKWTFSKALELREKMKNE
                     YPLELNRIENYLCLTEKDYAIWKNISDKILINKQKETDIIEQFEGYFDLKEYEITSVD
                     ENGMPQWPKGLNLSELNSTKLIKQPDVVMLMLMMPQEFSEQELKANYEYYEKRTMHKS
                     SLSPSMYSIMGMKVGDTHNAFSYFMKCINTDIKDNHGNSLLGLHAASSAGAWQTAVYG
                     FGGFSIDSQDRACFSPWLAPDLQSIEFSVVYKNSRIKVFYDHEKVVITSERDMQVKVY
                     SEVILLKSGIECEVRINK"
     gene            14920..15567
                     /gene="pgmB"
                     /locus_tag="PLU28_00060"
     CDS             14920..15567
                     /gene="pgmB"
                     /locus_tag="PLU28_00060"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0046352 - disaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HOG33485.1"
                     /translation="MSFIKHCIFDLDGVIVDTAKFHYLAWKRLAKELGFELKPIQNER
                     LKGVSRVQSLEILLSIGNIKASDAEKSRMADQKNEWYVQYISSMTPKDILPGAKEFIL
                     KVRENGIKTSLGSVSKNAALILEKIAITHLFDCIVDGTRITKAKPDPQVFLISCEELA
                     VTPSESVVFEDSIAGIQAALAGGMHSIGIGNAKILSMAERIIPDFTHADMSILVY"
     gene            15765..16643
                     /locus_tag="PLU28_00065"
     CDS             15765..16643
                     /locus_tag="PLU28_00065"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HOG33486.1"
                     /translation="MDWISGIQRAIDYVESHIMQSIDYEEVAKQAYSSNFHFQRIFSI
                     MCGFTLGEYIRMRRLSMAGNELASSSLRVIDVALKYGYDTPESFSRAFVRFHGVLPSQ
                     VKHGAVALKSFSPLRVKLVLEGGKMLNYRIETHNALQIICKKLYVSSKDEMTQEYISP
                     FWKKCIEDGTIQALCKYIPKENIFKDCIVGASFGMDASDPKFPYAIGAYYNGAQITDE
                     GLTVEEIPAHTYAVFPFSGKMPEDFIKLYQQICSEFFATGEYQPCGGTDFEAYPSADV
                     SDPNYSCEIWVAVEKR"
     gene            16694..17662
                     /locus_tag="PLU28_00070"
     CDS             16694..17662
                     /locus_tag="PLU28_00070"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOG33487.1"
                     /translation="MYNDTIKELERIINIKYTNISGIIVQKNSINLYEKYFNGYTADN
                     AVHIYSVTKSIFSALIGIAVDKGFIKSVDQKVLDFFPDYSILDGEKTIQTVTIKNLLT
                     MTAPYKYESEPYEEFFASANWMKTALDLLGGEKNAGTFMYSPIIGAHILAGVLVNATG
                     QSVLDFAMKNLFLPMQINVSRNIELRSKEEHIAVMNNKNTTGWAVDPQGINTASWGLF
                     LTPRDMMKVGQLYLNNGIWNGKQLISSRWIEESTTQKSWWNELSYGYLWWIIDSTEGT
                     FAALGQGGNVIYVNRTKKIVVCIASLLTQNEPDMIDFIKNQIEPLV"
     gene            complement(17760..20993)
                     /locus_tag="PLU28_00075"
     CDS             complement(17760..20993)
                     /locus_tag="PLU28_00075"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOG33488.1"
                     /translation="MDIITQIKQDPDRNFFISASAGTGKTFTLKEYYMSILNKNKNNP
                     QIVENILAVTFTNKAAAEMRERIMNEVEKKLSDRENPFFWHTVKINIARAWITTIDSF
                     YSKILRENNIAIGVDPNFTVINEVKRDREIERACYNTLKVLFMIYENREFFQELNLSD
                     DRAESIKKLTDELIMHKEKFVEAVEFIFNDMGFSKLKESLEQAVLKWRVTLAHSDTLD
                     ELNLDSQDTEKYIQASWLFKKTVLIAREFYLYYTSDQFTFDFKGIMEKVLQVFENEEI
                     LDRYREKFKYIIVDEFQDTDDMQSSIFNLIHTKDNHIFLVGDRKQSIYRFRGADLSVF
                     AAMEKEFESKMAHDEKYIKQALSKNYRSDGQIVDFSNYMAEKILFCSDNDDEYSRQMI
                     VVDNDLYNNILFKDQDLCGWEEKEEDTDVVPKLLNEDKKRVKVIIRTEKEEEKSKKDM
                     LKEKEIQTVAWTIIKLLGQELSFRERDENTGKILSKIRPVRLSDITLLMTQLSGYEEL
                     IRQEFTDKKIPFYIVSGKSFYDRPEIQTVFSAISAVQNPHNDFEFAKYMMSFCAAMQI
                     DELYGLLKHKNRNDSLFTAFSNNKDTFCDEQKKAFEVLKKYKDLKYFISPAALLKGMI
                     AELDYMAKVSGAQKDPRYAISNVKKLLSELEKYESMAQTYAELVKLLKKITDTDEAQA
                     SVEDEKSESVKVITIHKAKGLEFPITILAGLGEDAEKSNPNKQEYLDFSVMLDNGKRY
                     FLLKSRFYDENIKIKPDKGKARYNSKDDHIKQFIRNDFLDSTEVKRRMYVAVTRPREM
                     LIPIVVKNEKDVTDKKYSGFFSSIDSRYADIISVDGSSFDYQEASYAVKENQKEFAPV
                     NSLNLSDLSAMSYKQYIAPTYLINEIKKSEADADEESEKQTRSYQYDSVFGLNENLEK
                     GTRLHKLLQSVRSIGTLESLVKQGVFAQGFDRIPIIRHAFSEDVIMVKNEWRIVKHQV
                     IASSDNNPREYMFFGVVDKAVMTEKNGEKNIYIIDYKYSELAEKSKIEDYKFQIRFYL
                     YLLNDFAKAEKGYVISLKNNKVIEVDYDPDFEKKLIQIVRLSKKEQD"
     gene            complement(20999..24079)
                     /locus_tag="PLU28_00080"
     CDS             complement(20999..24079)
                     /locus_tag="PLU28_00080"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOG33489.1"
                     /translation="MARKVKVTVNRDGFAVINQYITELFLSENPGAVVLDREFLKVYI
                     ANEIDELIEKEKDDPELCRYARVLSKSGKSGEYILEIFEKKWEITRTEDINLVNESEI
                     YKEFEEAWQDESPVGRLYRKLEKTLESIVAKRTEGCASYDQVSVYKWFYSSYNPTNKK
                     SKLIISGFFDINPITFKAMIKLFDIFEDVDFYIWDKINDRSFEFLPSIYKFLKSQNFV
                     EENLSSQTFMKLEDLFSGTQSVSMRAVVSDDPITDIQSTAVQIKKKILYEGVLPQDIG
                     VVTPDMASAGLMADSLEKMSVPYRFKNDIPLSKSKSVSILVLPLKTVIRGCEVQDFIA
                     MIEAGFAGDTELAMEDVEKYFKRLGILYDDRKTAIKQKKFEWLGWIDEEYKRLNLLDN
                     EEEHERAVRELEDLSKLKLLMEKIFEVLDEIEHTPKVKSSWYSEKVKSWIENNVLGFE
                     VLKKYPDSQAVETETNAILCFIELLDKTEESLEKLLEREKDKTIKMEKFYYILSNLIQ
                     TKTFRSSQRYSNTVEIMDLLDSRFVNKKIKYFVNFSESSYPSIGINPLLIYLSCGGYP
                     FYMVQEKQARRNLLISLAFSQNVVISYPKASVTGEPILPSIYLKEIMEMSKIKYESEN
                     RKNIVPDSPDEIFSREDACIYCVANDVKYSGEDAKLKEMIRQSEYIRERAALKLWTLK
                     EPTKVDNFSHTKLSAYIDCPFKYFLKYSMLLKGDKEFDLFNEGLLKHKVLYDIFNTYP
                     SRNDLSDALSNDKNFSQWIYQIVQNRWIDQFKEGLYQYEIVRKQESQRIAQDLFSVVE
                     YISDGYVRLDKKNKKIRFAKVDALEKPFETSIVHNNDTIKINTRIDRIDMVDENYEYK
                     PSKTDTFLEKKEGAIGIIDYKNSEHFQSEQLFLYYKILNSLNYTEGEDVYLIFLVTKE
                     DKSNKSLVIKIQNNEVYMKKSGKSTEYVSFNFSEFDTWLSEVITDIKNSYFVPLAVFE
                     PKKKRFLIQMREKYENASSSESSYPCIGYEYSGCEYLKLCSLLGYHEDFSLKAR"
     gene            complement(24265..25665)
                     /locus_tag="PLU28_00085"
     CDS             complement(24265..25665)
                     /locus_tag="PLU28_00085"
                     /inference="COORDINATES: protein motif:HMM:NF025112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOG33490.1"
                     /translation="MDSRISFNLPVQVQTILDILSENKRQGYIVGGCVRDTVMGVTPN
                     DWDFATDAHTDEIIRVFSEKGFRVIPTGVKHGTVTVLIKSKGYEITTFRTDGEYSDYR
                     HPDSVDFVKNIEQDLSRRDFTINAMAYNPKDGLIDPFNGISDLMHNDFLRTVGDPERR
                     ISEDPLRMMRAVRFSVRFSKKFSPELEKAVSKRAYLIEKISRERVNDEFVKILLCQYG
                     ADGVRLLSDTGLMAYICPAFIRLKGLRQIDRYHGGEDVYTHTLRVIDYTYPKKTIRLA
                     AFLHDIGKYDTLSSDGMRTRFIGHEKISAEKAREFMNSMRFSGKDICDVANLIENHML
                     LKDYDTSKSENDKKTYLRKVIVKVGCENMSDFVQLCFADEKATPSCNMQKYEKYEEII
                     GLFEQIRNEKVFSAAELEIDGNDILALGVRKGPMIGEILEYLLEMVLKDPSINSRLEL
                     IKLAEQYIKAAETGGK"
     gene            25958..26950
                     /locus_tag="PLU28_00090"
     CDS             25958..26950
                     /locus_tag="PLU28_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOG33491.1"
                     /translation="MKKILSVLVIMVLSFTMLMAANSLKAYTTLEEPLAKALFEQFEK
                     ETSIHVEWVRLTTGEAVARLEAERDKPQASIWVGGVGTGHIDAKNKGLTTTFNSYVAQ
                     TVPDKYKDPEKYWIGLYIGPIAFATNTNRAKELGITSPKSWADLLKPDYKGRIRVANP
                     ATSGTAYNMVTNIIRISGEDETKAFDYLKKLNVNIDQYTKSGSAPGKSCAIGEIPVAI
                     GYAHDLIKLKVEGAPIEITFPSEGSGFETASMSLIKNGPDAVNAKKLYNWVLGSKSME
                     IIASWYVIPVSSNAPKDKLHFDINSITTVNQNDKWDAENKERLIDFWNKEIGTK"
     gene            27028..29217
                     /locus_tag="PLU28_00095"
     CDS             27028..29217
                     /locus_tag="PLU28_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOG33492.1"
                     /translation="MFTKKKVLIFIISLVLFTVVDFWVFSNIKDSFDDIMTQQIRKNV
                     SAAVESVPREEEEIPEWIQTTKNINQELEVFYLKGFEAKEILLGEEMQSFFDVHKLSE
                     DFSKGLESASYEEIYISPKKYDFKGKSYKLIFVPSIDHETAEFLGVGVFLYSSAEQDK
                     FYGLLYILALSSFGIFFLVYYIGRFTRDPVLGYTILILFAVVLIFVAYPLFEAVRLSF
                     IKEGRFSFEIWKNILTSKQYLSALWGSILLGICTATASTAIGFLFAFVLTRTNIKGKK
                     FFSAMATMPVISPPFSLTLSIILLFGNNGLITKQLLGLQNFNIYGLGGLTLVQTMGMF
                     PIAYLTMAGVMQAIDSTLEDAAMDLKANRWTTFKTITLPLATPGLLSSWLLVFTNSLA
                     DFANPLILSGNYRVLSVEAYIEVTGMNRLGNGAALSLLLLLPTLTAFFVQRYWVSRKS
                     YVTVTGKPSGRMRELTTKPVKVTLVSIMSFIAAFLIGLYGTIVAGCFVKNWGIDYTFT
                     LNNFKEALERGKDALIDTVTLSAVATPIAGILAMIVAVIVVRKTFIGKKALEVLIMTP
                     FALPGTLVGISYILAFNKPPIILVGTAAIIIINYVIRELPVGVEGGIATLKQIDPSIE
                     EAAQNLGADTPTIFRTIVLPLIRPAFISSLSYTFVRSMTAVSAIIFLISARWYHLTVL
                     IYNFSENLRFGLASVLATTLIVIVFVAFGLMRLLVRKNDNLEKNISA"
     gene            29231..30322
                     /locus_tag="PLU28_00100"
     CDS             29231..30322
                     /locus_tag="PLU28_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG33493.1"
                     /translation="MNKKSIPVKLDKVWKVFKDPQAKKDVVAVENADFTIQPGELVTL
                     LGPSGCGKTTTLRMIAGFELPTQGKILIGDSDVTYLAPNQRDTAMVFQSYGLFPHMNV
                     FENVAYGLKIRKVPKDEIKKKVTEFLGLVGLENLAQRSPSRLSGGQQQRVALARSLIV
                     EPSVLLLDEPLSNLDALLREQMRVEIRRLQKSLGITAVYVTHDRVEAMSLSDKIIVMN
                     SGKIVQIGPPHEIYRNPVSKFVAGFIGKVAFFQAQVMEVSDKCTVKINDGVYTISSFD
                     SSLKPGDRSVIMCRPEALTLSEPKKGLIDGTVVTNVYLGNSVESFVNTDIGEVLVQID
                     NPAEKRIFKEGERVSVTFLPNLTKVLKDS"
     gene            complement(30375..31538)
                     /locus_tag="PLU28_00105"
     CDS             complement(30375..31538)
                     /locus_tag="PLU28_00105"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG33494.1"
                     /translation="MVFSRFNIFEDSQEYSVVFNTFNKVVTVLSDQDSQKLRLDLKSK
                     VSIGEGLGKFLVEKGVLISKDEDELQKLMQKNHNNKDILNLYLVPTNKCNLNCRYCFM
                     KSNPSCSDSADLVSDTVEFLKDNVHKYNGINIVWFGGEPLLAIDKMEEITSALKNEIE
                     QNIFYSSTVITNGVLLDQANVLRLQEIGVKRVQIVVDCMLLGNDEYSSFGYEKFYSNI
                     KNVCGKMETILRVDYENIERAEVKSLFEDLIKSGYGDKVQIYLSSRNGAELSRAREYF
                     DSIIHALKKVVAMGMKYAYSVYNPSLACAVVYDGHYIVDSSGNIYKCALQIGKPQESV
                     GTLKEGIEEDRLSKWQLMNAFTETSCMSCSIYDTCKNNKDAKRWGLGLVNILV"
     gene            31851..33482
                     /locus_tag="PLU28_00110"
     CDS             31851..33482
                     /locus_tag="PLU28_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG33495.1"
                     /translation="MTHKRINYLSELNGSLSYGLFSKPFILYHIKNSSSPEWSLYMKA
                     MLNLYKARYDIAMEYINKLLELTDGSKYLYYLTLELKFQTVSMMSNFSDKETARSIYE
                     LLRKNLNKLNASLMSFIIPSVISYSFLYGDQTVKKFRSWSQKYKISANRHDFNKLRIV
                     CKKIKSMDTFSSYEYFQHNYDLLQENFHPSCLSTLLDTWASAFCIKDPEPALEKAKEA
                     VYLSAKYIDEVQFGFSELYTFFTVLKQNLLVYESYQTSQMLKELYGMLEQSSPGIDTS
                     KEYRKLMRHVDVFSEDIFSSSYNTDEYFNNLLQKYYSQKIISVSRTTLYNIIKKKKKA
                     DERTVSLVYKTAQINDTDKIITSATLIKFYIRTFIKKNFDIIYTPSDLQAKKLLLPKF
                     LLYFLSCSYKKEEIVFSSLLRKINSEKKQIFSMKEKKFIIDSVFDLSPWHKARMELIL
                     QFKSCLKKEKQEKFINTLVSLPFILRTLAVRFILNYERYRSIWKIQAQIPQALEKIIL
                     KNSLDRYACTAAFYCFDSREKEFFEKKIIPLFTKV"
     gene            complement(33632..34888)
                     /locus_tag="PLU28_00115"
     CDS             complement(33632..34888)
                     /locus_tag="PLU28_00115"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG33496.1"
                     /translation="MKLSRFNVIIDLKGQTLVYNSFSRGVILLDSDNAELLKGSISKI
                     LDNAELRCVLEENGMLVQDNIDELKMLKFVDRYQRMAGRVLSLVVAPTMECNLNCWYC
                     YEGSLKKGTMTQENKDRLLDFILGNLPDSKRLRIQWYGGEPLLALDFIRNVSEKIIGM
                     KDEISSFSAGMITNGYLLDEKTVLELKKLNVGHIQITLDGTRETHDRKRCLKNGKGTF
                     DRILENISFASGIIPLTIRVNIDKENFYEMDDLLDILQKNNLKDKISIYASSVKPSEY
                     AADNSCCMSESEYGKNELILLEKIIKRGFQYAYEIFNPLDSCAAVFDKYYIIDPHGSI
                     YKCPMQIGRADMSVGSIDKGLDYAKLSDWILYDPFQDVKCHECKVLPICKGGCPWARM
                     SGRSSSCSDYKSNIADYTRIWFSQSI"
     gene            35023..>35980
                     /locus_tag="PLU28_00120"
     CDS             35023..>35980
                     /locus_tag="PLU28_00120"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF012470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HOG33497.1"
                     /translation="MNKKFFLLFIVLLILPLTTLSTVKNIILFIGDGMGTAAVDLSRM
                     VLLGKEKFWDFENTRYIGLQKTYSENLLTTDSAAAATALSSGHKTSNGLLGTLSDATA
                     TKSILKAFREVGRSAGLITTVTVTHATPAGFCVSMPDRDELAIADEYAETKPFDLIMG
                     GGRQYFVPKSSTASKRTDNLNLIKNFQNFGYTYISNTAQLFELTGKTPKIIGLFSESS
                     MPNALDRNYLNTPVPTLAQMTRTALNVLSSNPKGFFLMVESGKIDFRAHANDTAGTVW
                     EVNELNEAFKEALSFLKKDPDTLILVTADHETGGLTLLYPRLQ"
BASE COUNT        11210 a         6849 c         6089 g        11832 t
ORIGIN      
        1 taattgatct ataaggtaaa aataacgata tgttttttaa aatatcagga taaaaatcta
       61 atggtattaa acttccggaa aaaaaagcac ttatgacact atttgcatat cttaaaccat
      121 tctgagatat tgtccagaaa ctaagtaaac ctattagtat tgatatctct gaatatatta
      181 ttatcgaaaa aataatgctt ataaaaaaca aagaataatt taatatatta aaacatatgt
      241 taatatcaaa aaatactaca ccataaataa tgattaacca tgaatttaaa aatattcttc
      301 ctagcgacaa accaatatct ctaaataaat aaagataaat ataatcaata ggtaaaatta
      361 aaaagttgta tatattcccg gttcttatgt ctttttcaat atccatactt gtagtatttt
      421 gagttattga ctttattatt gtagaaaaca gtaaataatt agttagtgaa ctaatagaat
      481 aattttttat ttgcgggcta ttttccaaaa tatatttcca aatatatgtt gaagctgtaa
      541 attgaaacaa taaagaaata atttctatga gaaatgagta cttataaacc aatacttttg
      601 aaaaattaat ctttataata gaaaaatact tttccaactt atttttcagc aatttaatca
      661 cctatctatg ttattggaat gatctttaat caaaatattc tgaataattt tttctaaaga
      721 agaatttgaa aagacaatat cttttatatt gtttttaaaa aggactaaaa gatcagaaat
      781 taagttttct aaattaattt tctttttgtc aaaagatatc tttatttttg aattattatc
      841 taatttttct atgttaaaca gattatattt ttcttttaat tgatttccat caaaatcatt
      901 ggcgggtttt gataaaatta agattatttc gttttctttt tcatacatat tctttaaagt
      961 ttggagagta tcactaaaaa taatttttcc cttatcaatt agaataatat tattgcataa
     1021 ttcttcaatt tcatataaat tatgagaagt aagtattatt gtagtattta aatctttttt
     1081 tatttctttt atcatttcca aaaaaatatt tttataatat atatctaaac ctaaagttgg
     1141 ctcgtctaga aaaagaagtt ttggcttatg aagtattgaa gcaatcagat tacatcttga
     1201 taattgccct aaacttaatt ttcttaccgg tttaaataat aaatcttcta aattccattt
     1261 tttgattaaa taatttaaat acttattaaa actatcctcc tctaaactat atattttcga
     1321 taacatttta taggattcta taacaggtaa gtcccaccat aactgaggtt tttgacctaa
     1381 acatactcca atatttttaa catatttttt tctatcagaa aatggattca atcccatgga
     1441 ttttatggtt ccgtctgtag gggacaatat accagtaagc atttttatta ttgtagtttt
     1501 accagcccca ttaggtccta gtaaacctgt aaccgaattg ctttttataa taaaacttat
     1561 attgtctact gctgcaatat taattttttc acgacgaata aatatatttt ttatatttcc
     1621 tataatgccc tttttattaa gatagacctt gtaagttttg cttaaattag tagcactgat
     1681 aatatcgtca gtcacaattt gcctccatag atattaacca acttcctgcc ctattaaatg
     1741 cacaaattaa agtatcaata tcaacgataa ctttttttgt tcccatctct gcaaacatga
     1801 ttttgttatt ataagctact gataccattt cacctgcgaa tcccacagtt ttttatagta
     1861 cccgtttttc cgaatcagtt cttcatgtgt tccatgttct tttatctttc cgtcgtccaa
     1921 cacgattatt ttatctgcta tacttacagc tccaagtctg tgtgtcacca aaaaaaccgt
     1981 cttatcattg aaaagctgta caaatttttt gtacagttct gcttcctgca aaggatcaat
     2041 tgcacttgtc ggttcatcca gcacaacaaa actgccgttt ttatatattc ctctggccat
     2101 agacaacctc tgccattctc cacctgaaat atctattccc ccaaactctt ttcccagttt
     2161 ttcgtccata gaatatcggt ttaagggaaa tccggattct tttaatgctt tcatctgctc
     2221 ttggagatct tttttttgag ggtttccaag ctgtacattc tctgcaactg tcattttata
     2281 tttgtttacg ttttgaaaaa cagcactctt tcctgcaaaa agatcttttt tttctatggc
     2341 tttggcctct ttgccgttgt aaagaactct tccctttata gggtcataaa gtcctagtat
     2401 catgtttaca agcgtacttt tgccggctcc gttttttcct actatcgctg ttttttctcc
     2461 tttttttact tccagagtta tatcttccaa tgcatttttc tctgagcccg gatacttaaa
     2521 agaaacattc tccagtctga gacttgtgaa cccacagtca catctttcgt ttcttttatc
     2581 tggaaatatc tccatgaacc taaaaaatgg ctttacatac gttgtaaact gatccagata
     2641 gccatacagc tcaaaaattt cttgtgtgta ctctgaaaga ctggtaaatg caaacaggct
     2701 tgctgtgaac tctccaagtc ttattttgcc tgttatcaac aagtatgccg aatacgcata
     2761 cgcaccgaag cttcccacaa atttcaaaag ttcggcaatg gcattgactg aaataacttt
     2821 tttatctgca ccccattctg ctgctactct tttttcaaaa ggtcccagcc atttgttttt
     2881 gagaaaagct tcaatagaga gtattctgac ttctttatta aaattcaggg aggtaagact
     2941 ttccttatat gccatttctt caagctgtaa aggcattgtc ttttctctgg tcttaccgta
     3001 ctgtcttgct gagtaaattt tttctaaaag taccgcgatg ttgcttataa taacaaaaat
     3061 aattaaggcc ggattaaaat ctttcatata aataagtact ccgatgcttc cgaacaaaat
     3121 gcccacaaga tagatgttta tctctataat tctgtataca ttagtgcttg ccgttcttgc
     3181 ctgaattatg taattatata attcaggatt ttcgtaattt acggcgttta tctcatcaca
     3241 tttgttatga agcatcaacc gtgcaagtct ttcaaatttc agcaacggcc cgtattgtag
     3301 aaagtatctt gtgtagtaaa taaggtggaa cttagtaaac agcaagtata ccccgtacca
     3361 tataaccaaa acaacaaatt tcgggatctt ttgggtattc ccgggaagta cctgaaccat
     3421 attaaaaatt tttacattca gcattatacc taaaagagga agagaataat atgcaatact
     3481 tacagcaagc attaaaacaa actctttttt catgatctga aaaccgatat tgaatgattt
     3541 tttaattatt tcatgattat attttctgtt tttcatataa actcctttga ctgtcatata
     3601 gtttataata ctctctgttt aatttcataa gactctcatg ggaacctttt tcaaagattt
     3661 tgccgttttt aaccaggaga atctcatccg aagatttggt tgagccgagt ctgtgcgtaa
     3721 tgaatatgac tgtttttcct tttaactttc tgttatacat ctcatatatt tctgattccg
     3781 ctataggatc aagactagct gtgggttcat caaatatttt taacgtttta tctgcgaaaa
     3841 gcagccttcc tatggcaact ttctgccact ctccacccga aagatcagac cctccagaaa
     3901 atttttttga aagtgttgtg ttgatgcctc ccgaaagctt tgacggtata tcctcaccgt
     3961 ttacttcgtt taaaagctga cttatatgat tatcatcaga cttttttgat aagtttccta
     4021 taagaatatt ttctttaacg gtcagcggaa atcttccaaa gtcctgaaac aacacagaaa
     4081 tgtttttcga tagctctttg cttgaaatac ttttaatagg aacaccgttt ataagtatct
     4141 ggccttcgac cggttcatat agacccaaca gaagctttat tatagtggac ttgcctgctc
     4201 cgttttcacc tactacagca taatgcctgc ctttttcaat tttaaagctg cagctgtcca
     4261 atataagctc tttactccct ggataatgga aactgacgtt tttaaactca accatctcta
     4321 cttcacttat ctctttacct tcattttctt catattcagc aagttccata aactctataa
     4381 agtccctgcg gaattttcta tatcctgtta attctggaat tttctcgaat cctgaattaa
     4441 cagaggcaaa aaccgttccg agagcctgcg taacagcaac ataatagcca agtgaaattg
     4501 tattcccaat aagcggaaaa gccaaaagaa taaaacatcc caccatataa attattccag
     4561 ttgtttcaaa taaagactgt attttaaatc ttttaagccc ggctttttta ttctccttct
     4621 gagccttgta aaactctttg ttatactcaa cgtttatata ctctgtatag ccaaaaatac
     4681 ttttatccag aacgtaagac ctttctgtaa gtatttcaga aagataatta tatctgcgtg
     4741 cattttttat atacttctgc caaaatccgt aaagtgcttt gcccgacttt atattcagaa
     4801 aaacacctaa aaatatgaaa acaactatac tcagcataat aaacagatta acttgcttaa
     4861 gcatgtaaat gactccaaaa aactgaacag cgctgaaaac aagagatgta ataatattca
     4921 tagtgctgat tatcttttcg tgggaaccgt ccagccttga gattaagtcc tgtgtctttt
     4981 gatcttcaag aacaggatac tttattttta tggtcttatc taaaatacat ttattcaact
     5041 cacaagacaa aagttttttc atgatcttat ccatccaaaa gctgataaaa ttaaccatac
     5101 cgggaattaa ataaaccagc gctattagaa acagataaaa aaagattaat tccatatttc
     5161 tttctgagcc agagtttatc acagcatcaa taaatacttt tttatacgtt ataccggcaa
     5221 cgaccaaaac acttactaat agacttagta ccagcttcaa cagaaaaata agctttatat
     5281 atagattatg agataagaaa taacctgttt ttcttctatt catggttacc tccaaaaaat
     5341 ttttttgaat tgagatcata aatatttgtt tttcattttt tattaatcat atttctcata
     5401 tttattatat cattttttta aaattgtttt aaattctaaa tatttataaa aaaatatctt
     5461 ttttgtctac cgtcctttag tgttggagcg ttactataaa agtagagtcg tcaaacaccc
     5521 caaaaattga taaaataaaa tcaaaagaag aggtgtttaa aaatgagaaa gtacagtaag
     5581 gaaccataaa aaaggaatta ttaaagccta tacttacaaa tgatgagata cgattacata
     5641 ttgaagacaa gtaaaaagaa taatattcag atatatcttc tgctattaca acacacagag
     5701 aattacaagc ttgaatcttg atgggatgac acctatagca tatcgtaagc ttacctgtga
     5761 atctgtagct taacaagcag actatgaaca aaagtatcta aacccttaga aatcattact
     5821 aagttacttt atattttggg tatttttcga ctatactttt cttgacattt ccagatcagc
     5881 tacaataccg ccatatttag atttccagct tttctgatgg ttcaatcctt ggggagaagg
     5941 tcaggagggc tcctgttccc cctattacca gataaaacag accataaaaa atataaacat
     6001 ataaaaaatg atgtataata aaattatatg agataataat gtaaattttt taatttataa
     6061 attgtttttt gtaaaaaaac ttcttataga aatgatatgg agttattaac agatgcgtac
     6121 tacactgcaa tatgaaacaa aatataagga ggggatagaa gatgaaaaaa cttcttatat
     6181 tatgtactgt atgtatgttt tttttactaa cctcatgtat aagtatattt gaagaacctg
     6241 cattggagtt atactggcat ctgtactttc ccggaaaaga gtataaatct cctgatcagg
     6301 gaacgtactt tacatacgat gaaacaaccg gctaccttta tgtaagattt tcaggtgaaa
     6361 gagtaggatg ctttgacctt gcaaaaaaga agactgtgtg gataaggtat ataaatagtc
     6421 gcggattgac acttataaat ctggacatag agctttataa aaatatggtt tacgtaatac
     6481 gtgacgatgt gagaaaaaaa gataaaaaag atcctgtatg tgccatctgt gctctggata
     6541 agcttaccgg agagacggta tgggagtatg cagttacaga tgaaaagttt aagtcaggca
     6601 taacggttca taatggtttt atatacattc cggattacga taaaagagac gggaaaaaag
     6661 gtatgctggt taaacttgac gcacagacag gaaaggtagt atggataaat aaaaatgttc
     6721 agccggttgg agtaaccaaa tcgttgatag acgaaaagta cagcctggta tatgtaggaa
     6781 catttgggaa tgaccatata ggagtagctc ccaaagttta tgctcttgac atagaaagcg
     6841 gagagacagt atgggaaaga aatattttat gggaagaacc tgtaatagta gatattgtaa
     6901 cagttcctgc agtatacgga gacagtgtat atgtcggtac atggcacgga gagtttgtaa
     6961 gactgagaag gacagacgga agagtagtat ggtctgcaga atatccggaa agaggatttg
     7021 gatttggtgc aaggatatgg gtatataaaa acacacttat atcatcattt gttgatggcg
     7081 gagtggtctg tatagaccct tctgacggaa agatactgtg gcatactaag aatcccgaac
     7141 cgacaataga agattgctat atgaaaggta acagagtata catacacgga gcaacaaaca
     7201 gcttcaaatg tttttcggca gacacaggaa aagagatatg gacatataca tactcaaact
     7261 ttaacggaac ggagtacgga cggatgggag gagcaccgtt taaagcagga aagtatttta
     7321 taacaggaga tgttggagga aatcttatga tatacataga aaagaagtaa gatgaaaaaa
     7381 cttcttatat tatgtactgt atgtatgttt tttttactaa cctcatgtat aaaagctcct
     7441 gaaaatgcct ttttagtaat atatatcatg gaaacaaact gcagtcagaa aataagcgga
     7501 gctgaaatta ccatatatat gcaaaataaa aaatactcat cagaactgtg tgaaacaggt
     7561 gtgtatgaaa tagatattcc tgtcagtaaa aaatatgagc ctatagatat ttttgtatgt
     7621 aaggcaggtt attccaacca tacgacatac ggtcttgaga tagaaaaaaa taaagtgaac
     7681 ctatttcggg tacttcttca caggaacgag ccattgaaga ttaaatgata cttattccca
     7741 atgaactata aaatcagtac tgaaattaaa ggcggacaac tactgtccgc cttttcacta
     7801 ctcttttatc caatcataaa cctctttttt tgttatggtt gcaagtgcta tgtagtggag
     7861 agttaccata aaagcagagt cgacaaatca ggacgaagta taatataatt atcttgtctt
     7921 tcaaaagcaa tattttttgt tcttggtcaa aatcttagta taagccatca aatcatagag
     7981 atacttatgt ttttttgctt taacgtcagt tattcaaagt taagagtacc tgattgaagt
     8041 tgttcaaggt cacaaaaaag tgatacaatt ttaatgtaat cgattacaag gggtgaaaaa
     8101 tgaacatcaa ggaaatagca aagcttgccg gcgtatctgt tgctacggta tcacgcgtta
     8161 taaatgattc caatgatgtc acggaagaaa ccaaggaaaa ggtcagaaaa gtaatttccg
     8221 aacataacta tgaaccggac tttattgcta aatcactcag aggaggaaaa acagacatat
     8281 ttggtgtaat cttccttaaa tcggattctg atctcaacga ttcgtattct acggcttttt
     8341 tgatgggtgt tttgaattat atgctccatc acggtaaaag aacaatagtt gaagcaagtt
     8401 ataaggagga caagcttaag gcttataaaa agcttatttc cagctctctt gtcgatggct
     8461 ttattctcct tgatcttgaa gatgatgatg aaagggtaaa gtatcttaaa tctataaata
     8521 ttccttttgt tgttgtcggt caaaactcta aaaacgattt catttttata gatactgata
     8581 atacaaacgg cggttacctg ggaattaaaa gtttatacag catcggatgc agaaatattt
     8641 tatatataag cggtactaaa ggtccatgtg tctcgtctca aagacttgat ggagttaaaa
     8701 aagcttgcgg tgaactcggt atttccgcag atattcagta cggagatttt gaaaaggaat
     8761 ctggctatag gatcgctaag gctactcagc tgataggata tgacggtata ttctgtgcat
     8821 ctgattttat ggcctacgga gtaatggagt tcatgaaaga gaaaaagatt tctttaccca
     8881 taattggttt tgatgatctg agcgaatcaa agatccttgg acttacatca atacgccagg
     8941 acataagaaa tgtaggctat ctctgtgcaa gcaaactcaa cgatattatc ttgggaaagg
     9001 aagcttcatc tcagatacta ccggtggaat gtatagaaag aagttctaca aaaaatttca
     9061 gaaaatcgca aaagtgattc atatttatta ggaggggtta tatatgagaa gggtaattct
     9121 ggtactaagc attattttta tcgtttcttt ggctttcagt tctcctgtaa aacttacaat
     9181 gtcaggatgg ccgggaaatc ctgatgaaga agcggcaata acagccggag ttgaagcttt
     9241 taatgcaaag cacagcgata ttcagcttat atgggaacct atacccggag attatttcag
     9301 tactttaaaa acaaggttgt ccggcggaac aggtcctgat atattttacg tggatgtatt
     9361 cgtttttgaa gaactggcta caaacaactc aatgctctct cttgatacat acataaaaaa
     9421 agatggattc aaactttcag attactataa ttcattggta gaggcctttc agtataagaa
     9481 tagaacctac ggaatagcaa aagatttttc aacacttgca ctttatttca ataaatctat
     9541 atttgacaaa tgtggagttg cttatcctac caatgatgag acatgggaca gtttacttat
     9601 aaaacttcag gaattaaaga aaaaaggtgt ggagtatcct ataattgtaa atccggaact
     9661 tacacgtatg atacctttta tcaactcttt cggcggaagt gttgttgaca gcaatatgaa
     9721 agtctcactc tctcagccta actcaaaaaa aggttttcag ttttacattg atcttgtaac
     9781 caaatataaa gtcggatatg aagcttcagc agcaggagca ggatgggaag gagaagcttt
     9841 cggtaaagaa aacgcagctg ttatgatgtc aggtccatgg tgtcttggat ttcttaaggg
     9901 ctcataccca aatgtttata ataaaatggg tatcgtagaa atgccaaaaa atgtaaaaag
     9961 atccactatg gtctatactg tatgctgggc aataaacaga cagtcctcaa ataaagatgc
    10021 ggcatgggag gcaatgaaat ttttggttac agaaggacag cagatatttg tcgaaaaggc
    10081 tggagtcctc ggctcaaaca aaaatgtagc agctaaggat acagacccaa taaagcaggt
    10141 tttttacaag ggggcagatt acgcaactcc ttggaagata ccgactccta caggtttgtt
    10201 ctccaaagca aacgatcaga taaattcgag aataaaggat ttattctata ataagataac
    10261 attggatgag gctttgaaac agataaacga aaattatgat tcctggataa agtagacaaa
    10321 ttaacaaaat ggtggagtac tccaccattt tgttttaaaa atgtttttga ggtgatcatc
    10381 cgatgaaaaa aagagttgtt caggaagcta tggtaggcta tctttttgcc gctcctatta
    10441 taatctccat atgtatattt acactttatc ctataatttc ggcatttata tacagtttta
    10501 ccgattttaa cccgcttgaa attaaaaaat ttgatataaa gttcaatccg caggatgcta
    10561 tagaaataaa cacgggcttt cttccttcgg aacttagcga aaaagaagtt gtcattgact
    10621 ttgaccttat gacttttata gagcttgacg tcggattgaa gcttgatgct gaaaaatctg
    10681 acctggtaca aaagtatttt gacagttcta agcttttaaa agatttttct gaaggaaaac
    10741 ttgataaaga gattgctata aaagacttca tgggcacata tatgaccatg catggtgatc
    10801 agttccgtaa gtacagacca aagtttcttt ggtttgataa cttcatggag cttttaaaag
    10861 atcagtactt ttggatatct cttggaaatg cctttttctt ctccttaatt gtagtgccta
    10921 tacaaacatt gctagctgta attcttgctg tggcggcaaa ctcaaaaata aagggagtaa
    10981 agttctataa agcagtattt tttattccat caataacttc ttctgctgca atatcaatga
    11041 tattctggat gatatattcc aaaccgggag ttatcaacag gctgtttggc acctctattg
    11101 actggcttaa tgaacctaat acagccctgc ctgccataat gctcatgaat atatggacta
    11161 ctgccggata ttttatggtt acattcttgg caggacttca ggatattccg gattcaatct
    11221 atgaagctgc agaaatagat ggtgcatcag gattgacaac cttttggaaa ataaccatgc
    11281 ctcttctgcg tccgcagata cttttcgtag ccataacagg caccattgga tgtatgcagg
    11341 tttttgacca gatatacttc cttataaaga atatcaggaa tataactatt tcatttttta
    11401 tctacaaaaa cgcctttgaa tacggaaaga tgggttatgc aagcgctgtt gccctcgttc
    11461 tttttctgat aatacttgct ataaccgcag tacagagaaa gtatatcaaa gatgagtact
    11521 aggagttgat attatgaagg tcaaaaaatc aagaataatc gcatacactg ttttgatagc
    11581 atatgtcata atttcacttt ttcctttttt ctgggcactt atggtatcaa taactcctct
    11641 taactactac gatactatca ctaatgaaca aaaaggcata gatataatgc aatggcctcc
    11701 aaaaattaac ttttttaaag gtgaagtttt tggagcacct atgacttttg aaaattatat
    11761 aaagattttt caggttgcgc cactctttgc aagatggata ataaatacct tcatatatgc
    11821 cgggttgata actctgggac atgtgcttat agataccttg ggcggatatg cctttgccag
    11881 actaaaattt ccttttaaag atctttggtt catgcttttc ctggcaacta tgatggttcc
    11941 gggtcagata ataatgatac ctcagtatac catgatggta aactacggac ttataaacac
    12001 atatgccgga ctatttctgc ccaaacttac cggggtattc ggtctgtttc ttctgcggca
    12061 gttctttctt aactttccaa aggagcttga agaagcagcc agaatagatg gagcaggaat
    12121 cataaaaact tactttaaag tcgttcttcc aaatgctaag cctgctgttt cggcgcttgc
    12181 tatctattct tttttgggtg catggaatga actgatgtgg ccgctgcttg taacttcaaa
    12241 aaaagaaatg tatactctgg caatgggttt aaatttcttt aaaacctcct actatacttt
    12301 ctggcagtac cttatggcag catctataat aatgacagta cccatgataa taatcttcct
    12361 ttctttccag aaagagtttg ttgaaacagg aagatcggca gctgtaaaag gatagctatg
    12421 ttattttctg tttataaaat aaaacaagaa ttgtgctaaa ttatgcacaa ttcttgtttt
    12481 atttcgttat ttacttatta attctaactt cgcactcaat accgcttttt aatagtatta
    12541 cttccgaata aacctttacc tgcatatctc tttctgaagt tattaccact ttttcgtgat
    12601 cataaaaaac ttttattctt gaatttttat agaccacact gaactctatg ctctgaagat
    12661 ccggtgcaag ccacggggaa aaacaagccc tatcctgaga atcaatacta aatccaccaa
    12721 atccgtagac tgccgtctgc catgctccgg cgcttgaagc agcatgaagt ccaagcagac
    12781 tgtttccatg attatctttt atatctgtat ttatacattt catgaagtat gagaaggcat
    12841 tatgagtatc tcccactttc atacccatta tggagtacat gcttggactt aatgacgact
    12901 tatgcatagt ccttttttca tagtactcat agttggcttt aagttcctgt tcggaaaatt
    12961 cctgcggcat catgagcata agcattacta catcaggctg ctttataagt tttgtagagt
    13021 taagctctga aagattcaac cccttaggcc actgtggcat tccattttca tcaactgagg
    13081 ttatctcata ctccttaaga tcaaaataac cttcaaactg ctctattata tcagtttcct
    13141 tctgcttatt tataagtatt ttatcagata tattcttcca tatagcataa tctttttctg
    13201 tcagacagag ataattttcg attcggttaa gctccaaagg atattcattc ttcatcttct
    13261 cccttaactc aagagccttt gaaaaagtcc atttgaccag atagtttgta taaaagttat
    13321 tgtccacatg ctcgtgaaat tcatcagggc cgatcacaga gtttatctcc cagatatcct
    13381 tattcttatt atataccgct cttcctgtcc agaaccggca gacttcaaat attatttcag
    13441 aaccgtattt aaacataaaa tctttatctt cggtagccct gtaatactca aaaatactga
    13501 aaactatgtc actgcttata tggacttcaa tgtcaccggt ccatatccgt acaggcttat
    13561 tgttataatc gtatccccac ttgggagttg tttccaatcc gtcatcagca gactcccaag
    13621 catactgggc gcctgaaaaa ccattcataa aagcattccg cctggcaccg tccagtgtat
    13681 tatatctgta catcaaaaga gctttagcta cctgcggctg tgtgtatata aaaaacggaa
    13741 gcataaatgt ttctgtatcc caaaaaacat gtcctttgta tccctctcca tggagtccct
    13801 ttgctcctat gcttacggtc ggatctttat catatgcgca cgaagaaaga tggaagatat
    13861 taaatctaag tgccttctga gcctcttcat ttccctgtat ttttatatca gagctattcc
    13921 aaaaaccttg ccatagctct gaatgttttt caagttcggc ttcatacccg tcagctataa
    13981 aagcttctat ttcccttttg caatccgttg aggcattgct gctttctctc gaagagtaca
    14041 cagctccgaa tttatataca acaatggttt ctccttttgc cgcaatgaca tcccctgctt
    14101 gctgaaccct gcactgaaga tcattgaact ttagacgtga aaattttttt tctttcagaa
    14161 ttatgcagga tgcttgggct atctcaattt ttttatcgtt tgttatagcc accatcatta
    14221 ctcccggttt cagatcctga acagaagaga ccttataatg agcagataat tctttgggat
    14281 tatgacctga atttaccgtt cctccatcta taatgctctc aaaaacaaaa agtgcttctg
    14341 aatccaaagc tgttacactg tacctgcaag cccacctgtg aaagtttttt ctgctgacaa
    14401 accgttcact ctcaatttgt acacgtagac ctttctgaac ttccacaata aaagcctctt
    14461 tgagaatacc atttcccata tctaagcttc tttgataatc gattacatta tttatatcga
    14521 gatctatctt ctgtccattg acataaatgt tcatcaaaag aggatttgga aggttaacaa
    14581 gttctgtcac ctgtgcagtg cttctatcaa aaataccggc tatgtaattg cctggattat
    14641 tgtataaaga aaattcatta tatcctctca ttcccatgta cccgtttgct aagttaaata
    14701 atgtaccgtt tttatagtta tcttccttaa tgtaatcctt aacagaaaac tcccatagat
    14761 ccttcatcaa aaattcctcc ctgttatgcc gtatttattg tcgtatctat ataactatta
    14821 taaatattga ttgttataat aacaacaatt ttttgttatg aaaaacttac ataaatttga
    14881 tactcttata atgaaatcgt ttacaggagg gacaacataa tgtcttttat taagcattgt
    14941 atttttgatc ttgatggagt aattgtagat acagctaagt ttcattactt ggcatggaaa
    15001 cgccttgcaa aagaactcgg ctttgaacta aagcctatac agaatgaacg gcttaaaggt
    15061 gtaagcagag tacaatccct tgaaatccta ttaagtattg gaaatataaa agcttctgat
    15121 gcggaaaaaa gtagaatggc agatcaaaaa aatgaatggt acgttcaata tatttcatct
    15181 atgactccaa aagatattct ccccggtgca aaagaattta tactcaaagt acgtgaaaac
    15241 ggaattaaga cttctcttgg gagtgtaagc aaaaatgcag cgttaattct tgaaaaaata
    15301 gcaataactc atctttttga ctgtattgtc gatggaacca ggataacaaa agccaagcca
    15361 gatccgcagg ttttccttat atcttgtgaa gagcttgctg tgactccttc tgaaagcgta
    15421 gtatttgaag attccattgc cggcatacaa gcagcacttg caggaggaat gcatagcatt
    15481 ggaataggaa atgcaaaaat actttctatg gctgaaagaa taatacccga ttttacacat
    15541 gcagatatga gcatacttgt ttattaagtc tgaacatccc taaggccctt cgttgatacg
    15601 aagggctttt atttggtaaa atctatcatt actctcagaa tttaacgtat acaaattttc
    15661 agaaggcttt caaaaatttt ttataggtat gcatcactga gcacaaaaag tcaagaaaca
    15721 aaaaaataat tcttctataa tagagtgcga aaggaaggtg aaaaatggat tggatcagtg
    15781 gtatccaaag ggcaattgat tatgtggaat cacatatcat gcagtccatt gattatgaag
    15841 aagtggcaaa acaggcttat tcttcaaatt ttcattttca aagaatattt agtattatgt
    15901 gtggttttac actcggagaa tatattcgga tgaggaggct ttctatggca ggtaatgaac
    15961 tcgcttcttc cagcctgcgg gtaattgatg tagcgttaaa gtacggatac gatacccccg
    16021 aaagcttcag ccgggctttt gtacgattcc acggtgttct gccttcacaa gtaaagcatg
    16081 gtgcagttgc tcttaaatct ttttcgccac tgcgtgtaaa actagttttg gaaggaggaa
    16141 aaatgcttaa ttataggata gagacacata atgcgctgca gataatatgc aagaaattgt
    16201 atgtttccag caaagatgaa atgactcagg agtatatctc tcctttttgg aaaaaatgta
    16261 ttgaagatgg aactatccaa gcactttgta agtatatacc aaaggaaaat attttcaaag
    16321 actgtatagt tggagcgagt ttcggcatgg atgcatctga tccgaaattt ccttacgcaa
    16381 tcggtgccta ttataatggc gctcagatta ctgacgaagg tctgacagtg gaagaaattc
    16441 ctgctcatac ttatgctgta tttccatttt cgggaaagat gcctgaagat tttattaagc
    16501 tttatcagca gatttgcagt gagttttttg caacaggaga gtatcagcct tgcggaggaa
    16561 ctgatttcga agcctatcca tcggccgatg taagtgatcc taactatagt tgtgaaatat
    16621 gggtcgcggt ggaaaagaga taacgcaacc gtaccatttt ccattttcac aaatcatttc
    16681 aggaggaaga tatatgtata acgataccat taaagagctt gaaaggatta taaacattaa
    16741 gtacactaat atttccggaa ttattgtcca aaaaaacagt ataaacctct atgaaaaata
    16801 ctttaatgga tatacagcag acaatgcggt tcatatctat tcagtaacca agagtatctt
    16861 ttctgcatta atcggcatcg ctgttgataa aggctttatt aaaagcgtag atcagaaggt
    16921 gctggatttt ttccctgatt attctatttt agatggagaa aaaacaatac agaccgtaac
    16981 tatcaagaac ctgcttacta tgacagctcc ttataagtac gagtccgagc cttatgaaga
    17041 gttttttgca agtgctaact ggatgaaaac tgcacttgat ctgttgggtg gtgaaaaaaa
    17101 tgccggaact ttcatgtact caccaatcat aggagcacat attctggccg gtgttttggt
    17161 aaatgctacg gggcaatcag tacttgattt tgctatgaaa aatctgtttc tgccaatgca
    17221 aataaatgta tctcgcaaca tagagctacg tagcaaagag gaacacattg ccgtcatgaa
    17281 caataaaaat actactggat gggctgttga tcctcaaggg ataaatacgg caagctgggg
    17341 tcttttttta accccacggg atatgatgaa agtaggtcag ttatacttaa acaatggcat
    17401 atggaatgga aaacagctta tttcatcacg ctggatcgag gaaagcacaa ctcaaaagag
    17461 ctggtggaat gagctttctt atggctacct gtggtggatc attgacagta cagaaggtac
    17521 ttttgccgct ttgggacaag gtggaaatgt aatttatgtc aatcgcacca aaaaaattgt
    17581 ggtctgtatt gcttcgcttt taactcaaaa cgaacctgat atgatagatt ttattaaaaa
    17641 ccaaattgaa ccgcttgttt gaagatattg tattgtttag agcagtgtaa gccttatttt
    17701 ctgtgcgtat gaaaagctgg attcttcaac ttttctcgta tgcttaacag ttttatggct
    17761 taatcctgct ctttttttga caatcgtact atctgtatga gctttttttc aaagtcaggg
    17821 tcgtagtcca cctctatgac tttattgttc ttaaggctta ttacataacc tttttctgct
    17881 ttggcaaagt cattcagaag ataaagataa aagcggatct gaaatttgta atcttcaatt
    17941 ttactttttt ctgcaagttc agagtattta taatctataa tgtaaatatt tttttcgcca
    18001 tttttctccg tcataaccgc tttatcgacc acaccgaaga acatatactc tctgggattg
    18061 ttatccgatg aagctataac ttggtgtttt actattctcc actcattttt taccataata
    18121 acgtcttctg aaaaggcatg acgtataatc ggtattctgt caaatccttg tgcaaaaact
    18181 ccctgtttta caagactttc cagtgtgcct atggaacgca ccgactgaag aagcttatgc
    18241 agccttgttc ctttctctag gttttcattc agtccaaaaa cactgtcata ctgatagctt
    18301 cttgtttgtt tctcgctttc ttcgtctgca tcagcttcac ttttttttat ttcatttata
    18361 agatatgttg gagctatata ctgtttatag ctcatggcgc tgaggtctga aaggtttagg
    18421 ctgtttaccg gagcaaattc cttttggttt tctttgactg cataggaagc ttcttggtaa
    18481 tcaaaagaac ttccgtcaac acttattatg tcagcgtacc tactgtcgat cgaactgaaa
    18541 aagccgctat atttcttatc cgtaacgtct ttttcatttt ttaccacaat agggataagc
    18601 atttctcttg gtctggtcac tgctacatac attcttcttt ttacctcggt actgtcaaga
    18661 aaatcatttc ttataaactg ttttatgtgg tcatccttac tgttgtacct tgcctttccc
    18721 ttgtctggtt ttatctttat gttttcatca taaaaacggc tttttagaag gaaatagcgt
    18781 tttccgttgt ccagcataac tgaaaaatca agatactctt gtttgtttgg gttacttttt
    18841 tctgcatctt ctcccaaccc ggcgagaatg gttatgggaa actcaagtcc tttggctttg
    18901 tgtatggtaa tcactttgac actttcggat ttttcgtcct ctacagatgc ctgagcttcg
    18961 tctgtgtcgg ttattttttt tagaagctta acaagctctg cataggtctg tgccattgat
    19021 tcatattttt ccagttctga aagcaatttt tttacatttg aaatagcata cctcggatct
    19081 ttctgtgctc ccgaaacctt tgccatatag tcaagttctg ctatcatacc ttttagcagt
    19141 gcagcaggac ttataaagta cttaagatcc ttgtattttt taaggacttc aaaggctttt
    19201 ttctgttcat cacagaaagt atccttattg tttgaaaatg ctgtaaaaag tgaatcgttc
    19261 ctgtttttat gttttaatag cccataaagt tcatctatct gcattgctgc acaaaaggac
    19321 atcatgtact ttgcaaactc aaagtcgttg tgaggatttt gaaccgctga gatggcagaa
    19381 aatacggttt gtatttccgg tctgtcataa aaagattttc cacttactat atagaaaggt
    19441 atttttttat ccgtaaactc ctgtcttata agttcttcat atcctgaaag ctgtgtcatg
    19501 agaagggtta tgtcactgag tcttacaggc cttatctttg aaagtatctt tcctgtgttc
    19561 tcgtcccttt cacggaatga caactcttgt ccaagaagtt tgataattgt ccaggcaacg
    19621 gtttggattt ctttttcttt cagcatatct tttttacttt tttcttcttc tttttcggtg
    19681 cgtattatta cttttactct ttttttatct tcgtttaaaa gtttaggaac aacgtctgtg
    19741 tcttcttctt tttcctccca gccgcaaagg tcttggtctt taaacaaaat attattgtaa
    19801 agatcgttgt ctacgactat catctgtcgt gagtactcat cgtcattgtc agagcagaaa
    19861 aggatcttct ctgccatgta gtttgaaaaa tccacaatct gtccgtcaga tctgtaattt
    19921 ttactaagcg cttgttttat atacttttca tcatgagcca tctttgattc aaactctttt
    19981 tccattgcag caaaaaccga aagatcggct cccctgaatc tgtatatgga ttgttttctg
    20041 tctcctacaa gaaaaatatg gttgtccttg gtatgaataa gattaaatat actgctctgc
    20101 atatcgtcgg tatcctgaaa ctcatccact attatgtatt taaacttttc cctgtaacga
    20161 tcgagtatct cttcattctc aaaaacctga aggacttttt ccattattcc tttgaagtca
    20221 aacgtaaatt ggtctgaggt atagtaaagg taaaactctc tggctatgag tactgttttt
    20281 ttaaatagcc atgaagcctg aatatatttt tctgtatcct gtgagtcaag attgagttca
    20341 tctaaagtat ctgaatgtgc aagggtaact ctccatttaa gaactgcctg ctcaagactt
    20401 tctttgagtt tggaaaaacc catgtcattg aaaataaact caactgcctc tacaaacttt
    20461 tctttgtgca ttatcagttc atctgtaagt ttttttatac tttctgccct atcgtctgaa
    20521 agattaagct cctgaaaaaa ctctctgttt tcataaatca taaaaagtac cttaagtgtg
    20581 ttataacacg ctctttctat ctccctgtca cgttttactt cattaataac ggtaaagttc
    20641 ggatcaacgc ctatggcaat attattttct ctgagtattt tcgaatagaa gctgtctatg
    20701 gttgttatcc aggctcttgc gatatttatt tttacggtat gccaaaagaa agggttctct
    20761 ctgtctgaaa gttttttttc tacttcattc attattcttt cgcgcatctc agctgccgct
    20821 ttattggtaa aggttaccgc cagaatattt tcgacaatct gaggattgtt tttattttta
    20881 ttgagtatgc tcatatagta ttctttaagg gtgaatgttt tgccggtacc tgcagaagcg
    20941 gaaataaaga aatttctgtc cggatcctgt tttatctgtg taattatatc cattctaatc
    21001 accttgcctt gagtgaaaaa tcttcatgat accccagaag tgagcaaagt ttgagatact
    21061 cacatcctga gtattcataa cctatgcagg gataactgct ttcggaggag ctggcgtttt
    21121 catacttttc tctcatctgg ataagaaatc ttttcttttt gggctcaaat accgccaacg
    21181 gaacaaaata agaatttttg atgtctgtga tcacttcgct taaccatgta tcaaactctg
    21241 aaaagttaaa gctcacatac tctgttgact taccgctttt tttcatgtat acctcgttgt
    21301 tctgtatctt tatcacaaga cttttatttg atttatcttc ctttgtaaca agaaaaataa
    21361 gataaacatc ctctccttcc gtatagttaa gagagttaag tatcttatag taaagaaaaa
    21421 gctgctcgga ctgaaaatgc tccgaatttt tataatcgat tatgcctatt gcaccttctt
    21481 ttttttctaa aaaggtatcg gtttttgaag gcttatactc atagttttca tcaaccatgt
    21541 ctattctgtc tattctggta tttattttta tggtgtcatt attgtgtact atgcttgttt
    21601 caaatggttt ttcgagtgca tcgacctttg caaaccttat ttttttattt tttttatcaa
    21661 gtcttacata gccatcagaa atgtattcaa caactgaaaa aagatcctgg gctattctct
    21721 gtgactcttg ttttcttact atctcatact ggtaaaggcc ttctttgaac tggtcaatcc
    21781 accggttttg aacaatttga tatatccact gcgaaaaatt cttatcgttg gaaagagcat
    21841 ccgaaagatc attccttgaa gggtatgtat taaaaatatc gtaaagcacc ttgtgtttca
    21901 aaagtccttc attgaaaagg tcaaactctt tatcgccttt gagcagcata ctatacttaa
    21961 gaaagtactt gaaaggacag tcgatatatg ccgaaagctt ggtatgtgaa aagttgtcaa
    22021 cttttgtagg ttccttaagc gtccataact tcaaggctgc tctttctctt atatactccg
    22081 actggcggat catctcttta agcttcgcat cctcgccgct gtactttaca tcgtttgcta
    22141 cacagtaaat acaggcatct tcacggctga atatctcatc gggactatcc ggaactatat
    22201 ttttgcggtt ttccgattcg tactttatct ttgacatttc cattatttct ttaagatata
    22261 tgcttggaag tataggctct ccggtaacag aggcttttgg atatgagatg acgacatttt
    22321 gggaaaaagc aagtgatatg agcaggttac gtctcgcctg tttttcctga accatataaa
    22381 acgggtatcc tccacaggaa agatagataa gaagtggatt aatgccgatt gacggatagc
    22441 tgctttcaga aaaattgaca aagtacttta tttttttatt tacaaaacgc gaatccaaaa
    22501 gatccattat ctcaacggta tttgagtagc gttgagagct tctgaaagtc tttgtttgta
    22561 taagatttga taggatatag taaaactttt ccatctttat agttttatct ttttctcttt
    22621 ccaaaagttt ttcgagcgat tcttccgttt tatcaagaag ctctataaaa caaagtatgg
    22681 catttgtttc tgtttctacg gcctgactgt ccggatattt tttcaaaact tcaaaaccaa
    22741 gcacattatt ttcgatccag ctttttactt tttcactgta ccatgatgat ttaacttttg
    22801 gggtatgctc tatctcgtcc agaacttcaa aaatcttctc catgagaagc ttaagctttg
    22861 aaagatcttc aagctctctt acagctcttt catgttcttc ttcattatca agaagattaa
    22921 gtcttttgta ttcctcatca atccatccca gccattcaaa ctttttttgc ttgattgcgg
    22981 tctttctgtc atcataaagt attcccagtc tcttgaagta cttctctaca tcttccattg
    23041 caagttctgt atcgccggca aaacctgctt cgatcatagc aatgaaatcc tgaacttcac
    23101 aacctctgat aactgtctta agagggagaa caagtattga tacactctta cttttagaaa
    23161 gcggaatatc gtttttaaat ctgtatggga cagacatttt ttcaagactg tcagccataa
    23221 gtcccgcact ggccatatcc ggagtaacca ctccaatatc ctggggcagg actccttcgt
    23281 acagaatttt tttctttatt tgtactgcag tactctgaat atcagttatt ggatcgtctg
    23341 ataccaccgc acgcatactc acgctttgtg ttccggaaaa aagatcttca agcttcataa
    23401 aagtttgaga ggaaagattt tcttccacaa agttctgact tttaaggaat ttgtagattg
    23461 acgggagaaa ctcaaagctt ctgtcattta tcttgtccca tatatagaaa tcaacatctt
    23521 caaagatatc aaaaagcttt atcatagctt taaacgttat gggatttata tcaaaaaatc
    23581 cggatatgat cagtttactt tttttgttgg taggattgta tgatgaatag aaccacttgt
    23641 aaaccgaaac ctggtcgtag cttgcacaac cttctgttct tttggcaact attgactcaa
    23701 gagttttttc caactttcgg tatagccttc cgacaggact ttcgtcttgc caggcttctt
    23761 caaactcttt atatatttct gattcgttca caagatttat atcttcggtt ctggttatct
    23821 cccacttctt ttcaaatatt tcaagaatat attctcctga ttttccggat tttgaaagta
    23881 ccctcgcata ccggcaaagc tccggatcgt ccttttcttt ttctatcagt tcgtctatct
    23941 cgttggctat atatacttta agaaactccc tatccagaac cacagccccc ggattttcac
    24001 tcagaaaaag ctccgttata tactggttga taaccgcaaa gccgtccctg ttaacagtaa
    24061 ccttgacttt ccttgccacc tcatcggcca actgttttac ataaaagcca gacggcccta
    24121 taaactgaaa gctaaaaggg tctgatttat aatcatctgt tatgtattca ctcagatctt
    24181 taaaatgatt tctgttgatt ggaattatcc ttaaccttct gataatatct cctcccgcct
    24241 actaaagatg cttaaaatca tatgtcactt accgcctgtt tccgctgctt ttatatactg
    24301 ttcagccagt ttaataagct ccagcctgct gtttatggac ggatctttca gcaccatctc
    24361 cagaagatac tccagaatct ctccgatcat aggcccctta cgaactccaa gggcaagtat
    24421 atcgttgccg tcaatttcaa gctctgcagc tgaaaaaact ttttcatttc ttatctgctc
    24481 aaataatcca attatctctt catacttttc atacttctgc atgttacatg acggggtagc
    24541 tttttcatct gcaaaacaaa gctgcacaaa atcactcatg ttctcgcagc caactttaac
    24601 aattactttg cgcagataag tttttttatc gttttcagat tttgaagtat cgtaatcttt
    24661 aagaagcata tgattttcta ttaaattagc cacatcacag atatcttttc cagaaaatct
    24721 catactattc ataaactctc tagccttttc tgcggaaatt ttttcatgtc ctataaacct
    24781 tgtccgcata ccgtcggaac ttaacgtatc gtactttcct atgtcatgca aaaatgccgc
    24841 cagacggatg gttttcttag gatatgtata gtctatgacc ctcagagtat gagtatagac
    24901 atcttcacca ccatgatatc tgtctatctg cctcaaacct ttaagcctga taaaagcagg
    24961 acagatatac gccatcagtc ctgtatccga aagcagcctt actccatcag ctccatactg
    25021 acagagcaat atttttacaa actcatcgtt tactctctcc cttgatattt tttcaataag
    25081 ataggctctc tttgaaacag ctttttcgag ttccggtgaa aattttttgg aaaaccttac
    25141 cgaaaacctt actgccctca tcattcttaa aggatcctcg cttattcttc tttcaggatc
    25201 accgactgtt ctgagaaaat cattatgcat aagatcgctg attccgttaa aaggatctat
    25261 aagtccatct ttcggattat atgccatagc gtttatagta aaatcacgtc ttgacagatc
    25321 ttgttctata ttttttacaa agtccacgct gtcaggatgc ctgtaatcac tgtactctcc
    25381 gtcagtcctg aaagttgtta tctcataacc tttacttttg attagtacag tgaccgttcc
    25441 gtgtttaaca ccggtaggaa taacgcggaa ccctttttct gaaaaaactc ttataatctc
    25501 gtccgtatgg gcatcagtgg caaagtccca gtcatttggc gttactccca taaccgtatc
    25561 acggacacag cctcccacaa tatatccctg tcgtttattt tcgctaagaa tatcaagtat
    25621 agtctgtacc tgcacaggta agttaaagct tatcctgctg tccatatata tcaccgctca
    25681 tcccgtataa aaaatcaaaa gtattatttt tatttgaact actttatcac tttaaataat
    25741 aacataatta ataccatgtt ctttcaatta tttggtactt gttatgccgg ttgtttttgt
    25801 ttattaagat ttattaagaa tctgatttac aatcaaattt taaccttgat tttggtcata
    25861 aacgcttcag agtaaatttc atcaggattt tttttcttcc gcaaatgata tataatataa
    25921 agaagatgta tatattattt aaagaggggg caaacaaatg aagaagatcc tgtctgtatt
    25981 agttatcatg gtgctttctt tcaccatgct tatggctgca aacagcctaa aagcatacac
    26041 cacgcttgaa gaacccttgg ccaaagctct ttttgaacag tttgaaaaag agacctcgat
    26101 tcacgttgaa tgggtaaggc tcactacagg tgaagcagtt gcaagactcg aagccgaaag
    26161 agataaaccg caggcctcaa tatgggtagg aggagtagga acaggacaca tcgacgcaaa
    26221 aaacaagggt ctaacgacta cattcaactc ttatgtagca cagacagttc cggacaagta
    26281 caaagatccg gaaaaatatt ggataggtct ttatatagga cctatagcat ttgccaccaa
    26341 taccaacaga gctaaggaac ttggaattac ctcgccaaaa agctgggcag atcttctaaa
    26401 acctgattac aaaggaagaa taagagttgc caatcctgca acatccggta cagcctacaa
    26461 tatggttacc aacataataa gaatcagcgg cgaagatgaa acaaaagcct ttgattatct
    26521 taaaaaactt aatgtaaata tagaccagta caccaaatcc ggttcggctc cgggaaaaag
    26581 ctgcgctata ggagaaatac cggttgcaat aggttatgcc cacgatttaa taaaattaaa
    26641 ggtagaaggt gctcctattg aaattacctt cccatctgaa ggatcaggtt ttgaaaccgc
    26701 ctccatgtca cttataaaga atggtcccga tgcagttaat gctaagaagc tttacaactg
    26761 ggttcttggc tccaaatcaa tggaaataat tgccagctgg tatgtaattc ctgtttcttc
    26821 aaacgctccg aaggataagc tccactttga catcaactct ataacaacag tcaatcaaaa
    26881 tgacaaatgg gatgcagaaa ataaagaaag acttattgac ttttggaaca aagaaatcgg
    26941 aacaaaataa atccagaaaa atcagacggt cctaaaacag gaccgtcttt tatagtactg
    27001 cgctagatat tttagggagc tgatagtatg tttactaaaa agaaggttct gattttcata
    27061 atctcactcg ttctttttac agttgtcgat ttctgggtat tctcaaatat aaaagattcc
    27121 tttgacgaca taatgaccca gcagataaga aaaaatgtat ctgcggcagt tgaatctgtc
    27181 ccccgtgaag aagaagaaat acctgagtgg atacaaacca ccaaaaatat aaaccaagaa
    27241 ttggaagttt tctatcttaa aggttttgag gccaaagaaa ttcttttagg agaagaaatg
    27301 cagagttttt ttgatgtcca taagctctcg gaagattttt ccaaaggtct tgaaagtgcc
    27361 tcttatgaag aaatatatat ttctccgaaa aaatatgatt ttaaaggtaa atcatacaaa
    27421 cttatctttg taccttcaat agaccatgaa acagccgagt ttctgggtgt cggggttttc
    27481 ctatacagct ctgctgaaca ggataaattt tatggcctgc tttatatact ggctctgtca
    27541 tcttttggaa tttttttcct tgtctactat attggtcgtt ttacacgtga tccggttctt
    27601 ggctacacaa ttctcatcct ttttgcagtt gtacttattt ttgttgccta tcctcttttt
    27661 gaggcagtac gactttcatt tataaaagaa ggacgttttt cattcgaaat atggaaaaat
    27721 attctgactt caaaacagta tctttcggca ctgtggggaa gcatactgct aggaatatgt
    27781 accgcaacag cttccacagc cataggcttt ctttttgcat ttgttcttac caggacaaat
    27841 ataaagggca aaaagttttt cagtgctatg gcgacaatgc ccgtaatatc gccgccattc
    27901 tcgctaacac tttcaataat actgttgttt ggaaacaacg gtcttatcac caaacagctt
    27961 ttgggattgc aaaacttcaa catatacggt ctgggcggac ttactcttgt acagaccatg
    28021 ggaatgtttc ctatagccta tcttacaatg gcaggagtaa tgcaggccat cgactccaca
    28081 cttgaagatg ctgcaatgga tcttaaagca aacagatgga ctacctttaa aaccataact
    28141 ctcccgcttg caactcctgg acttttgagc tcatggcttc tcgttttcac aaattccttg
    28201 gctgatttcg caaacccctt gatactttcc ggaaattata gagttctttc ggtcgaggca
    28261 tacatagaag ttaccggcat gaacagactt ggaaacggag ccgcgttatc acttctgctt
    28321 cttctgccga cgctaactgc gttttttgtt cagcgttact gggtatccag aaaatcttat
    28381 gttacagtaa ccggaaaacc atccggaaga atgcgtgaac tgaccacaaa acctgtaaaa
    28441 gtaaccttag taagtataat gagctttatt gcagcttttc ttataggctt gtacggaacg
    28501 atagtggccg gatgctttgt aaaaaattgg ggcatagact atacctttac cctaaataat
    28561 tttaaggaag cacttgaacg tggaaaggat gctttaatag atactgtaac cctttctgca
    28621 gtagctactc ccatagccgg tatactggcc atgattgtag ctgttattgt tgtaagaaaa
    28681 acattcatcg gaaagaaggc tcttgaagtt cttataatga ctccattcgc tctgccggga
    28741 acacttgtgg gaataagcta tatactggct tttaataaac ctcctataat tcttgtcgga
    28801 acagccgcta ttattataat caactacgta atacgtgagc ttcctgttgg agtcgaaggt
    28861 ggaatagcca cattgaaaca gatagacccg tcaattgagg aagctgcaca aaatcttggt
    28921 gcagataccc cgaccatatt cagaactatc gtgctgccac ttataagacc ggcttttatt
    28981 tcaagcctct cttatacttt tgtaaggtct atgacagctg taagtgctat aatatttctt
    29041 atttcggcca gatggtacca tcttaccgta cttatctaca acttctcaga aaatctgcgt
    29101 ttcgggctgg caagcgttct tgcaacaaca cttatagtga tcgtatttgt tgctttcgga
    29161 ttaatgcggc tccttgtaag aaaaaatgat aaccttgaaa aaaacataag cgcttgagtg
    29221 aggtgcagct atgaataaaa aatctattcc tgttaagttg gacaaagttt ggaaagtttt
    29281 taaagatccg caggcaaaaa aagatgttgt cgcagttgaa aatgcagact ttacaataca
    29341 acccggtgaa cttgtaactc ttctggggcc ttccggctgt ggaaaaacta ctacccttag
    29401 aatgattgca ggctttgaac tgcctaccca gggaaaaatc ctgatcggag attccgatgt
    29461 aacatatctt gctcccaacc aaagggatac agctatggtt tttcagagtt acggactgtt
    29521 tccacatatg aatgtctttg aaaatgtcgc ctacggtcta aaaatacgta aagttccaaa
    29581 agatgagatt aaaaagaaag taaccgagtt cctaggcctt gtcggtctgg agaatcttgc
    29641 gcaacgctca ccatcaagac tttctggagg acagcagcag agagttgcac ttgcacggtc
    29701 gcttatagtt gaaccttcgg tacttttact ggacgagcct ctttcaaatc tcgatgcttt
    29761 acttcgtgaa cagatgagag tagagatacg gagacttcaa aaatctctgg gaataaccgc
    29821 agtctatgtc acacatgaca gagtcgaagc tatgagtctt tctgacaaga tcattgttat
    29881 gaacagcgga aagattgttc agataggacc tcctcatgaa atttatagaa atcctgtatc
    29941 aaagttcgtt gcgggattta taggaaaagt ggcgtttttt caggctcagg taatggaggt
    30001 ttcagataaa tgcactgtaa agataaatga cggagtttac acaatttcca gttttgacag
    30061 cagtttaaag ccgggagaca ggtcagttat aatgtgccgt ccggaagcac tgaccctttc
    30121 agagcctaaa aaaggactca tcgacggaac tgttgtcact aacgtatatc ttggaaacag
    30181 tgtagaatct ttcgttaaca ccgatatagg agaagtactt gtccagattg ataatccggc
    30241 agagaaaagg attttcaaag aaggagaaag agtttcggtt acattcctgc ctaatcttac
    30301 aaaagtactt aaagattcat aacccaaaaa tttaatcctc ccgtacaaat acgggaggat
    30361 tttttaattt atagctacac taaaatattt acaagaccca atccccatct tttggcatct
    30421 ttattgtttt tacaggtatc gtatattgaa cagctcatac agctggtctc agtaaatgca
    30481 ttcataagct gccactttga tagtctgtct tcttcaatac cttcctttaa agttcctact
    30541 gattcctgag gctttcctat ctgtaaagcg cacttataaa tattaccgga tgaatcgaca
    30601 atataatgac catcatacac aacggcacaa gcaagagaag gattataaac agagtaagca
    30661 tacttcattc ccatggcaac aacttttttc agagcatgta tgatactgtc aaaatactca
    30721 cgtgctcttg aaagctcagc accattgcgt gaagatagat aaatctgaac tttatcacca
    30781 tatcctgact ttataagatc ttcaaaaaga ctttttacct cggccctttc tatattttca
    30841 taatctaccc taagtatggt ttccattttt ccacatacat tttttatatt tgaataaaac
    30901 ttttcatagc caaaactact gtactcatca tttcccaaga gcatacagtc aactactatc
    30961 tgaactcttt ttacacctat ctcctgcaac cgcaatacat tggcttggtc aaggagcact
    31021 ccgttggtta ttactgttga agaataaaat atattttgtt ctatctcatt ctttaatgcc
    31081 gaggttattt cctccatttt atctatagca aggagaggct cacctccgaa ccagactata
    31141 ttaatgccgt tatacttatg tacattatct ttaagaaact caaccgtatc cgacaccaga
    31201 tccgcactgt ccgaacagga tgggtttgat ttcatgaaac agtacctgca gttcagattg
    31261 catttgtttg taggaaccag atacaaattc aaaatatcct tattgttatg gtttttctgc
    31321 attagcttct gaagctcatc ctcatccttt gaaatcaata ctcctttttc aacaaggaac
    31381 tttcctaagc cctcaccaat actgactttg gactttaagt caagccgcag cttttggctg
    31441 tcctgatcgg aaagcacagt aacaacttta ttaaatgtat taaaaacaac tgaatattcc
    31501 tgagagtcct caaaaatatt gaacctggaa aataccataa agatcagcct ccgaaagatg
    31561 ccggatgata tagtataatt attatagcaa taaatttatt ttgaacaaat taacactttg
    31621 ttttttgttt tatatctaaa aatgcctatt ttatagaact tacatggtat gtctttttat
    31681 aaaatattgt taactatgaa catattcttg ttaaaccctt tatttaatat ctttttaaga
    31741 atagcatttt atattaatag ctttttttcg attattactt taatgttgat tgtttattaa
    31801 gtacaccctt taaaaatcgt tttttgatta attaacggag gatacgaccc atgacgcaca
    31861 aaagaattaa ctatctttct gaacttaacg gcagtttatc gtatggtcta ttttcaaaac
    31921 cttttattct ttaccacatc aaaaattcat cctctccgga atggtcactt tatatgaaag
    31981 ccatgctcaa cctttacaaa gcaaggtatg acatagctat ggaatatatc aacaaactgc
    32041 ttgagcttac tgacggttca aagtacctat actatctgac acttgagctt aaatttcaga
    32101 cagtatcaat gatgagcaat ttcagcgata aagaaactgc aagaagtatc tatgaattac
    32161 ttcggaaaaa ccttaacaaa ctcaatgcct ctttgatgag ctttatcata ccgtccgtca
    32221 tttcctatag ttttctatac ggcgaccaaa ccgtaaaaaa gttcagaagc tggagccaaa
    32281 agtataaaat atctgcgaac agacatgatt tcaacaagtt acgaatagtc tgcaaaaaaa
    32341 taaaaagcat ggatactttt agcagttatg aatactttca gcataactac gatcttttac
    32401 aggaaaattt tcatccttcg tgtctttcta cccttcttga tacgtgggca tcggcttttt
    32461 gtattaagga tcccgaaccg gctcttgaaa aggcaaaaga agctgtatac ctttcagcaa
    32521 agtatataga tgaggttcag tttggatttt ccgaactcta tacatttttc actgttctta
    32581 aacagaatct tcttgtttat gagagctatc aaacctcaca aatgcttaaa gagctgtatg
    32641 gaatgcttga acagagttca cccggaatag atacttcaaa agagtaccga aaactcatga
    32701 gacatgtcga cgtattctcc gaagatattt tttcaagctc ctacaatacc gatgaatatt
    32761 ttaacaatct gctccaaaag tattactcac aaaaaatcat ttccgtttca agaaccaccc
    32821 tctataacat aatcaaaaaa aagaaaaaag cggatgaaag aaccgtaagc cttgtctaca
    32881 aaacagcaca gataaacgat acagataaaa taataacctc cgccacacta ataaaatttt
    32941 atataagaac ctttatcaaa aaaaactttg acatcattta cactccatca gatcttcagg
    33001 ctaaaaaact tctcttacca aaatttttat tatactttct ttcctgttct tataaaaagg
    33061 aagagattgt tttttcatct ctgcttcgaa aaattaactc agaaaaaaaa cagatttttt
    33121 ccatgaaaga aaagaaattt atcatcgact cggtttttga tctttcccct tggcataaag
    33181 caaggatgga attgatactt cagtttaaat cgtgcctaaa aaaagaaaaa caggaaaaat
    33241 tcatcaatac gcttgtaagc ttgcctttta tactaagaac tctggcggta agatttatac
    33301 tgaactatga acgatacaga agtatttgga agatacaagc gcagatcccg caagctcttg
    33361 aaaaaataat cctgaaaaac tcactggaca gatatgcctg tactgctgcg ttctactgct
    33421 ttgattcgcg ggaaaaagaa ttttttgaaa aaaaaatcat acctttattc acaaaggtat
    33481 gactctaaaa actattattt aattatctgc cgcactgagg tccttctaca aagatgatca
    33541 ccataataca cgaacctgcc gcttctttct tttcaatcat acttttcaca gtctgtgcct
    33601 gctttagaac tttcatgatt tcatctcctt attatatact ttgactaaac caaatcctgg
    33661 tatagtctgc tatattgctc ttgtaatcgc tgcagcttga ggatcttccg ctcattcttg
    33721 cccatggaca tccgccttta cagataggta aaaccttaca ttcatgacac ttcacatctt
    33781 gaaaaggatc gtataaaatc caatctgaaa gtttcgcata gtcaagacct ttatcaatag
    33841 agccaacaga catatcagct ctgcctatct gcataggaca cttgtatata cttccatgag
    33901 gatctataat atagtatttg tcgaaaacag ccgcacaact gtcaagagga ttaaaaattt
    33961 catatgcgta ctgaaatcct cttttaataa ttttttccaa aagtataagc tcattctttc
    34021 catactctga ttcactcata caacaagagt tatccgcagc atattctgaa ggcttaacag
    34081 atgaggcata gatacttatt ttgtccttca gattgttctt ctgcaaaata tcaagaaggt
    34141 catccatctc ataaaaattt tctttatcta tatttaccct tatcgttaac gggattatac
    34201 cgctggcaaa cgaaatattt tcaagtatac ggtcaaaggt accttttccg ttcttaagac
    34261 acctttttct gtcgtgagtt tccctcgtac catcaagagt aatctgtata tgtccgacat
    34321 ttaatttttt cagctcaagt acggtttttt catcaaggag atacccgttg gttatcatac
    34381 ctgctgaaaa agatgaaatt tcatccttca taccaataat tttttcggaa acgtttctta
    34441 taaaatccaa agctaagagc ggttctcctc cataccattg aattctcaat cttttagaat
    34501 cgggaagatt acccagtata aaatccaaaa gtctgtcttt attctcctgt gtcatagtac
    34561 cttttttaag agaaccttca taacagtacc aacagttcag attacactcc atagtcggag
    34621 caactaccag actaagcaca cgtccagcca tcctttgata cctatcaaca aacttcagca
    34681 tttttagttc gtcaatgtta tcctgcacaa gcatgccgtt ttcttccaaa acacacctaa
    34741 gttcagcatt atcaagtatc ttgcttatac tgcctttaag cagctctgca ttatcgctat
    34801 caagaagaat tacccctctt gagaagctgt tataaacaag agtttgacct tttagatcta
    34861 taattacgtt aaaccgtgag agtttcaatg tcttctacct ccggtatatt tatcgcctgt
    34921 tattatacat agcaaagtta tgatatataa tatcattata gcactttaaa atatattatt
    34981 tgcttaacaa acaaatatat ctgaaaatat ggagggacac ttttgaataa aaaatttttt
    35041 ttgcttttta ttgtactttt aattttacct ctcacaacac tttcgaccgt aaaaaacatt
    35101 atactcttca taggagacgg aatgggaaca gcagctgtag atctttcacg aatggttctg
    35161 cttggaaagg aaaagttttg ggattttgaa aataccagat atattggtct gcaaaaaaca
    35221 tactcagaaa atcttcttac aacggattca gcagctgcgg caactgccct ttcaagcggt
    35281 cataaaacct ccaacggcct tttgggaact cttagcgacg caacagccac caaaagcatt
    35341 ctaaaagctt tcagagaagt cggaagatct gccggactaa taactactgt cactgttaca
    35401 cacgctactc ccgcaggatt ttgtgtcagc atgccagaca gggatgaact tgcaatagct
    35461 gacgaatatg cagaaaccaa gccttttgat cttattatgg gagggggaag acagtacttt
    35521 gtgcctaaaa gcagtacagc cagcaaaaga accgataact taaatcttat aaagaatttt
    35581 cagaactttg gatataccta tatatcaaat actgcccaac tgtttgaact cacaggaaaa
    35641 acaccgaaaa taatcggcct tttttcagaa tccagtatgc ccaacgcact tgatagaaat
    35701 tatttaaata cacctgttcc tactctcgca cagatgacaa gaacggctct aaatgtatta
    35761 agttcaaacc caaaagggtt tttcctgatg gttgaatccg gaaagataga tttcagagca
    35821 catgcaaacg acactgcggg aacggtgtgg gaagttaatg aacttaacga agctttcaaa
    35881 gaagcattga gtttcctaaa aaaagatccg gatactctga tcctggtaac cgcagaccat
    35941 gaaaccggcg gacttaccct actgtatcca agactccagc
//
LOCUS       DAOSHR010000002        31795 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_1099_length_31795_cov_5.666194, whole genome shotgun sequence.
ACCESSION   DAOSHR010000002 DAOSHR010000000
VERSION     DAOSHR010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 31795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31795
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1099_length_31795_cov_5.666194"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3875
                     /locus_tag="PLU28_00125"
     CDS             <1..3875
                     /locus_tag="PLU28_00125"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOG33498.1"
                     /translation="DTARKEISSYLEGSASSAAIAVRIDGQTVYSESFGVCDLNTLRP
                     VERDTQFNIGSVSKVFAAASVLILVQQGKVNLDLPVFIYLPEFSMKDPRYKDITVRML
                     LNHTSGIPGTSIKDSMTAQKNTGYIQQVILNLKNSYLKHDPGKISIYCNDGFTLAQAL
                     IEKVSKTSYSDFLTENIFSKLDMSNSTTYFKENNENIARYYDHDQNISFPAEYVNSLT
                     SGGLASTAEDLVKYAEILYNDKILNEQLRKEFMSPQAGSKTALSGEPLYMFGLGWDSV
                     EDSVFHSSGVTVLTKSGGTCFYSAQLHVVPEYKISVALTVSGRVDVKSINDVVLTEVL
                     KQKGILAGSKKVFNIQTVLPLSFFNGYEGIYASLESIFKYEIDLQNNGMNVEIFNGSG
                     FVPYDFLRYEDGYFISRSSDVYSFISDDYGKYIQQHFNDRKSTRVLIEKIKPSENIDV
                     KEFENKVWIPLNLKTQDLCVMMARTGSVGQAEGYAYLQFSGFHMNVLKDVFTGENNLR
                     YIRDTVAFRIEKTAGSCNLLFEGYRYADASNLPVLDRNEVIKIGLSGENISRRVSDDL
                     LLKFDIQDGARAVIFSDSLEMIYDSLIHDYREVYVPKGSYVTFICEPGAQVGINLKKY
                     QQDDRYRQVKARAEKIADTLIDNYGVTSLQYALIYNNELILSSFAAGTDSEKAEELST
                     STPYGIGSTSKMFTTAAIMYLVDKGLVELDKPVSEYIPQFTMKDERYKKITVRMLLNH
                     SSGLMGSIYAQGPTYNHPDTTNHDNILKELSGQMLKADPGAFSVYCNDGFTLAELVVE
                     KVSKMSFTEFIRVFIINPLGMKNTFTPQDSFEKSGLAATFMNGVRTPSETFNMIGTGG
                     MYSTAEDLCRLGQIFMDDSGYSPAGLILSRKAKDETFSKEYSKGVWPVQTDNIAGFGL
                     GWDSVDLYPYSSYSIRAVCKGGDTGLYHSSLVILPEYNIAFAATFSAGSSTLGTVFAQ
                     EIILDLLQAEGKIDRSSEKDKKSPAVLADMPLYMSEFSGSYENNENIYDIQISKNGVL
                     TERTIYPKGLPEHEYKYTKEGLFVNDAGNKKLSFVKESNGITYICTEKNMVLNGLGRV
                     KVKTYSAQKTHFSDVADGVKKVWEERTKNRYFLVNEQSTSQNYHQLAAASMSFSMDSE
                     LKGCISNYRIINNNFAVVNVQIPGFDGRDTQSIEVYEKDGKEYIKNAGWIFICDKNIE
                     YLNGSETGSCVIGTDGYACWYKIPDEKAGKTIEVKKQEGSSFAVYDEQECVFYSVACG
                     NKPVILPKNGMILFAGEKPGDVLYYSIK"
     gene            3899..4780
                     /locus_tag="PLU28_00130"
     CDS             3899..4780
                     /locus_tag="PLU28_00130"
                     /inference="COORDINATES: protein motif:HMM:TIGR01250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase-family hydrolase"
                     /protein_id="HOG33499.1"
                     /translation="MKEGFIETKNGKVWYCSFSSAKQTVPILVVHGGPGFVSMPQVIE
                     EFSDDHDVYFYDQIGSGKSDRASCNDFYSVENFVDELEEVIKALKFEQVILMGFSWGC
                     ALICKYMLCKKPENVKGIILSAPYLSSPRWYADQRENIMNMPEDTRKAILLGEETQNY
                     GDNYQKAMMEYYKKHVCCLNPWPDSIQQAFGKLNMDVYLTMWGPSEFTITGKLKDFDL
                     LPELERIDVPVLLTCGDYDEAEIKTVRDYQRAFKNASMAVIPMASHLHQIEQPEIYKT
                     VVRAFLKKICDPAALKT"
     gene            4777..7050
                     /locus_tag="PLU28_00135"
     CDS             4777..7050
                     /locus_tag="PLU28_00135"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HOG33500.1"
                     /translation="MITFKEDVFKIDTDSSSYVFGLNHGMLESMYYGKKIRDGSDYYA
                     LREKSSGGYPNTVLTENDDKTFVMDNLCLEYSFPGKGDFRNTAIEIRMPDSSFVCDFR
                     YSSHKIYDGGYKIPGLPFAIKGDQTLEVNLIDLSGLELILYYTVFSKTDVIARSVCIK
                     NGADKDIYIRKIMSMQLDLSDSFKLLTFNGLWARERHKDEKNLSYGEVVCSSVTGASS
                     NRQSPFMVLADRECTQSSGCCIGYNLIYSSNHYESVQVSGYDKTRFMGGINPAFFEWR
                     VGPNDTFFTPQAVISFSSCGLNGLSQNMHGFVRENIIRGEWAKKERPVLINNWEATYF
                     NFDEKKLLEIAKVSKDLGIELLVLDDGWFGLRNDDTTSLGDWYENRKKLPGGLSSLAE
                     KINEMGMMFGLWFEPEMVSPISDLYAIHPEWAVKVPSRKPSLGRNQLVLDLTRKDVRD
                     YIVNAVSNILNSANIQYVKWDMNRHITDAYSQELGARQGEFYHRYILGLYDILERVCG
                     AFPHVLFESCASGGDRFDLGMLCYMPQIWCSDNTDYYERVLIQEGTSYGFPLCTMGAH
                     VSAVPNHQTMRTTPLESRFNVACFGLLGYELDPTKLSDNEKLAVKEQIKFYKKYRKLF
                     QFGDFFRIKDGFESEHAVWEVVSADKKQSISGFFQKLFVPNPPSDKLFFKGLDEELFY
                     KISCREQYLEFVPGRLSDDEGFLSNKEEYGGYGDLLMNGGIKLNQQYPSIRYNQGSRL
                     MGDFGSRLYIARSEEEK"
     gene            7047..7874
                     /locus_tag="PLU28_00140"
     CDS             7047..7874
                     /locus_tag="PLU28_00140"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOG33501.1"
                     /translation="MKILSIIGASTFGNTTEVLRYFKSQLEKCGGYEFEDLYLQDHKI
                     DFCTGCHSCIMLGQEKCPHYLAVKEIEDKMMQADGVILATPGYMFSVSGIMKNFLDHV
                     AYNCHRPKYFGKKIFLISSCTKWQEEGVFIPMKTWAQGAGFDLCGSMYIDMMPFPYSE
                     GELEKRRNKVNEAAFSFDKIIKEKKEIKPEFSRIMIFHAFRTMSAMFPKVMQADYRYF
                     TSKNAYDKKTKWYIPAKISFLKNFIAGMIEKKMKSSIMDMIDKEKAEKAEGVFRNKL"
     gene            complement(7871..9043)
                     /locus_tag="PLU28_00145"
     CDS             complement(7871..9043)
                     /locus_tag="PLU28_00145"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG33502.1"
                     /translation="MKNLFLLIVVYMAFISLGLPDGIFGVAWPSIRIDLGQPLEAAGI
                     ISVILMLCSALSSFMNGRIVRKTGTGKLVLISCIMTGSALLGYSFIPSFAWLIFLAFP
                     LGFGQGAVDSSLNNYVANHYSSRHMSWLHCFWGVGAMSGPLILSAVMRGGGTWRKGYL
                     SICLIQLLLAVLFFISLGLWQESTKKEKEDVPSSHETKKKFLTDISPWLSIFMFFVYT
                     GAEYTVGLWAGSMLIESRGLTGETAGLWISFYYGSIMLGRFLTGFVVNKLGNRFMIKA
                     GLILSAFGIVLIFFRTCPALTMIGLIMCGLGFAPIYPCMMHETPKRFDAGTSRSLIGY
                     QVGAACIGGSFITAGLGVLFSKITLELMPLALLVLIACMFIASELLNKKTPVKESI"
     gene            complement(9231..9998)
                     /locus_tag="PLU28_00150"
     CDS             complement(9231..9998)
                     /locus_tag="PLU28_00150"
                     /inference="COORDINATES: protein motif:HMM:NF025626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaP domain-containing protein"
                     /protein_id="HOG33503.1"
                     /translation="MQDEKIITLNEQNIDSEHICCAIGNDAENLSRASMKKEWLKKEF
                     SKGHVFKKFDVRGKCFIEYCPAEQAWFPINADGYTFIQCFWVSGKFKGTGMGSKLLEE
                     CEKDSLNTNGLVIISSKKKKPFVSDKKYMLSKGFSVCDSAAGDFELLVKKFIPQAADP
                     KFTENAKQGTIEESQAMTFVYSDMCPFAPVYSQIMAKTAQKMGVTVKTIKLSGPEHAR
                     QTGALYGIFSLYDKGKFITNELMTEEKFTDYLKKLGY"
     gene            complement(10096..10977)
                     /locus_tag="PLU28_00155"
     CDS             complement(10096..10977)
                     /locus_tag="PLU28_00155"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOG33504.1"
                     /translation="MIRKAILVLGILIIGLSAFSLHMENSMIYGESGESVPVASYKKI
                     VVIDPSSVEVLYLLGAQESIAAIGHTAKSPIWPYDKTPALASVGSITKPSLEKVLSYT
                     PDLVLLNAMIGDFGLSLKQHSINYLIVNADSVEDILDNLEILGVLCGKEKEAITLSQE
                     KKSKLKKIKADILKNPLNMKGIFLYSTNPIMAFNSTSLSGQILEVIGVKNIAADLSAD
                     KPILSAEYILQQNPDFIIGAMSITSPQQIKDASPLIKSTSAAKKDKIFLIDSSKILRP
                     SPRIVDEIENLYLELKK"
     gene            complement(11005..12303)
                     /locus_tag="PLU28_00160"
     CDS             complement(11005..12303)
                     /locus_tag="PLU28_00160"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG33505.1"
                     /translation="MFEERLRSHHDSQRFVDDLLGMDFPKEHSDISKLDDFLCNNQDS
                     KPKVLYIHVPYCDRLCTFCNLNRELKKDSLSGYAQYVVNELNILSQKKYIKNAVFSAV
                     YFGGGTPTTFEPQDLEKILCTINTVLNLSDDCEFTFETTLHNLSDEKIQVMSKHGVNR
                     FSIGIQTFSSSGRKLLNRMYTPEYAVNRIKEIKKSFKGNVCLDIIYSYPYESIGEVEN
                     DALLCGSVDADSVSFYSLMIHNGSALYKSITEGKIIFERNIEQDKHNHNTFFRKMTEN
                     GYDLLELSKLAKPGRDRYRYIKMRYANADTVPVGVGAGGSIGRYGIFNMAPGRKMIVT
                     YHPVYAFFNRLLGYFQYGTYEDEFLRNYLNEDAYNAVCEKIETYMEEGFFIQSDKGIF
                     KLNEDGIFWGNNIAAQIMKSAIENTVKTADIKNYSETGIL"
     gene            complement(12296..12934)
                     /locus_tag="PLU28_00165"
     CDS             complement(12296..12934)
                     /locus_tag="PLU28_00165"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG33506.1"
                     /translation="MRGINIKGMKQAEMAKIVSFIPQKSSLSADLTVMDLIMMGRVVH
                     IKNKWVGFAKHDKEIAEHLIKKLNLEKFADRSALSLSGGEFQRVLLARALAQEPEILL
                     LDEPTSSLDMKYCMEIMAIVKELTAKKELTALAVLHDLNMTAMFCSEIFFMKDGSVRY
                     HGTPQQVYTREIIKDIYGIDATILKTDEDIPFIVPKTHPAISAKKEKEMAYV"
     gene            complement(12937..13119)
                     /locus_tag="PLU28_00170"
     CDS             complement(12937..13119)
                     /locus_tag="PLU28_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG33507.1"
                     /translation="MWDISTKELCFAYGGKINILDNMNVSIQKHSFSAVMGPNGCGKL
                     PSYETYLTFILRRKEV"
     gene            complement(13119..14111)
                     /locus_tag="PLU28_00175"
     CDS             complement(13119..14111)
                     /locus_tag="PLU28_00175"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOG33508.1"
                     /translation="MRKRILLFLIFFIIVFLIIFSLMNGAVKTSLRDIMDVLFNRSAN
                     TPDYIRTIIMDIRLPRIVMALLVGMMLASTGTVVQTVYQNPLADPYIIGISASATFGA
                     VLAFILKVPDLMYGVFAFVSCVITSFLIFRLSNAGKKLNTATLLIVGIAVSSFLGAFT
                     SFAMYMIGEDSFKIIMWTMGYLGGATWSKILFMIVPLVFSITVFLYFRNDLDLLMSGE
                     EEAHSMGVNVSRLKKSMLVITALVVAFSVAFSGMIGFVGLIVPHTVRLLFGCSNKKLI
                     PLSTITGGLFLLACDTISRLILAPMEIPIGIITSFFGAPFFLYLAFNKKGGMNR"
     gene            complement(14327..15664)
                     /locus_tag="PLU28_00180"
     CDS             complement(14327..15664)
                     /locus_tag="PLU28_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009537685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOG33509.1"
                     /translation="MSIKEQLLSNKPKDLMFQMSVPAVIGMVVIGLYPLMDGIFAGNI
                     IGERAMSAISIASPLTLLNNGVATLIGIGSASILSRAMGKNDQNTVDKIMGNLIAWIT
                     IFSIAITVFVLVFAETFLKIVGATPEIAEMGLRYLKVTILGSLFVNFTQSANMVMRGE
                     GLMKKAMIIMGLGAAINIILDPILMTVMGEKAIEGAALATVSAQILQAIATIYYFIKK
                     SPRVRIHRIKTEKSISKEMFSVGSSAMLMQLLFMLQQTMLYKMAFKYGGESNGILMAA
                     SLRFYGFSFIPLWGMSQGLQPIIGTNFGAKKYLRVKETMKVFMIGGACLAAIFWIPAQ
                     LFPNIVLRIFNVSQSIINEGIFNFRLFYSVFILYGIMVMSITFFQSIGDGKKAGIIVL
                     FRQLILFVPCMLVLPNMFGISAVWFTQPLVDLIMILIGLQMQMKTLKKMVAYN"
     gene            complement(15706..16707)
                     /locus_tag="PLU28_00185"
     CDS             complement(15706..16707)
                     /locus_tag="PLU28_00185"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG33510.1"
                     /translation="MKNYNKIKSMKVHEMLNVYPYSSEFFNINGLPQAGEEVSVHEYF
                     SSLPYSVLQDIGASRDELKKRLDLFIDTMQNMFSIGMPDIKTVTIVGGTAKDGTVENI
                     SLTVGIGEVLCIAGNTGSGKSRLLADIEWLAQADTPTKRKILVNGRPFPDSMRFSAEN
                     KPVAQLSQNMNFVLDVCVKEFIELHAQSLIDKKTAEKTKNIIQSANELSGEPFDLSSP
                     LTSLSGGQARALMVADIAFLSRSPVILIDEIENAGIDRKKALRLLSSNKKIIIIATHD
                     PLIALSADKRAVMKNGRIEKVIKTTPFEKQTLSRLTEIDEFIEKKRKALRNGDFLRE"
     gene            complement(16694..17485)
                     /locus_tag="PLU28_00190"
     CDS             complement(16694..17485)
                     /locus_tag="PLU28_00190"
                     /inference="COORDINATES: protein motif:HMM:NF014542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOG33511.1"
                     /translation="MGLLSLSSCFTAKERKKKYKEEIDIRFFTFSGPPSSGKTSVIIK
                     TALELKKKKYKIAVVKFDCLATDDDKLYAKNGIESITGLSGNLCPDHFFISNIDECFN
                     WAYAKPYDILISESAGLCNRCSPHIEGCTAVCVIDNLLGINTPRKIGPMLKTSDITVI
                     TKSEIVSQAEKEIFAFKIRQANPNAIILHINGITGQGANRLSDLLEKTPQIDSLEGKE
                     LRASMPSALCSYCMGETFIGKDKQKGNVRRINFDSIKGEAVSEKL"
     gene            complement(17427..18449)
                     /locus_tag="PLU28_00195"
     CDS             complement(17427..18449)
                     /locus_tag="PLU28_00195"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOG33512.1"
                     /translation="MKNSEFFFYSNRYRLLIIVAVAVVLFLIILFSLFTGTIKFSFQD
                     ILEIFGKNSSESMAKQILVNIRLPRILTGLFVGMNLSVAGVLLQGVLRNPMASPNIIG
                     VNSGAGLAAVIIMTIFPGNVSLIPPASFIGALAASMLIYLLSYSTQNSRTVYVILAGI
                     AVSNLLNALTSALMSLNSDTLEITYSWLLGSLSARGWTAFNSIWPYSLIALTVSIFIS
                     PKINLFGLGEEVASSVGLSTRFYRTIILLTSSILAGSAVSAAGTIGFVGLIAPHCARI
                     IVGNDHRFLVPLSALIGAALLILSDTVARTLFQPVEISVGIITSLLGAPFFIFLLYRK
                     RKEEKI"
     gene            complement(18487..19467)
                     /locus_tag="PLU28_00200"
     CDS             complement(18487..19467)
                     /locus_tag="PLU28_00200"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOG33513.1"
                     /translation="MFKFFSKKTVLLVCLAIALTGTAITGFSSTDTVRDLQNKPQIVK
                     TVFKDCLGREVTIQANPKRILALTSAAMQALYSINIEPVGKVDDYTITEEGKKLPGVG
                     MSKSINIEAVYSLKPDLILASSRFHANLEKELAQCGAVIFYFDPENIGDIPLIGMSTY
                     FGALLDREELAYKYVLSVFETAEKLKSQLATTNIKTGVVLQATDTITAAQSATSYGSM
                     LSLLGIENIVPDGLPNASKSSFVAFDIEAIIKSNPDIIFIVAPSNNQENNRKLLERYK
                     KDSKWSNLKAVQNNRILILPFSINPNRSLPEAMLSKTAEVILNNEKAFNK"
     gene            complement(19491..20084)
                     /locus_tag="PLU28_00205"
     CDS             complement(19491..20084)
                     /locus_tag="PLU28_00205"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOG33514.1"
                     /translation="MFKSNFDMLAPIYPLLIQQFVDDYNLDTGLAIDIGTGPGFLGIE
                     LAKITKMEIIFTDIDSDAIALANKNFIQSGTENGAQFVTANVENLPFPDNYADFIMSR
                     GSIWFWKDSCKGLLEIERVLKPGCYAVIGGGLGRYAPETMRKRLLTSMHKELEKKNEK
                     RPSLDEFKLMVAASGLKKFRVFTDGDVKSGKWVELNK"
     gene            complement(20077..21012)
                     /locus_tag="PLU28_00210"
     CDS             complement(20077..21012)
                     /locus_tag="PLU28_00210"
                     /inference="COORDINATES: protein motif:HMM:NF024739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOG33515.1"
                     /translation="MRFMIHTPVNLRGYMLDYVNTNLEFLEQKIGEKIEVFTPHDKLY
                     SSQCTEQWLGRAFENDVVPDLMLTHATEFASFSASQRESFLSSSACRYTQKNPLRKEF
                     LPFTAHNCFFYPAFIVPLIMFYNPYKIKKSDLANSWTDLFNKKFKVLFPARDKPLSRV
                     AGAYLNSLSSKDFQELQTRITYEGSPANVIRMVCEGDYDISINTLSFALMTKNQNIAL
                     NCPKEGFLILPQVFAWKKESSEKLDFIADLFMTEDFHRYLNDQEVWSCKDTLRMSGSE
                     LYGAILKNWKGWDVFLREVSDFDNYDCSPKEKKDV"
     gene            complement(21030..21821)
                     /locus_tag="PLU28_00215"
     CDS             complement(21030..21821)
                     /locus_tag="PLU28_00215"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG33516.1"
                     /translation="MIYILITRNLSFKYTDHMVLNSINFYAEKNQIISLIGPNGSGKS
                     TLLRCLCGLIRTEKNSVFLYEKPIESLSPKEIAQKIAFLPQFHQKLQGVSVQELVSMG
                     RSPYHKSGWILTSLDKQKIFSAMQYMQVEHLKNRPLEMLSGGEKQRAWIAMVLAQDTE
                     FILLDEPVTYMDLRYQQDLLRVVKNLKAHFQKTVIAVFHDINHAIEVSDFVYLLKDGH
                     IHNAGTSEDVITEKNIKDVYGICTHICRFKKCRRRVVVPAEVDNF"
     gene            21970..22482
                     /locus_tag="PLU28_00220"
     CDS             21970..22482
                     /locus_tag="PLU28_00220"
                     /inference="COORDINATES: protein motif:HMM:NF024055.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOG33517.1"
                     /translation="MRSLLLYSTLTGNTEKVAKAVYETMPQGTELKKASNSEEPSDYD
                     VIVIGFWVDRGTADEKILKFVEKTRGKKVAFFFTLGAYPDSPHAVKTAKDFEELLKKN
                     DNEVLGYFMCQGRIDPKLTEKFKDLPKDHPHAMTEERRKRHEEASKHPDENDLTAAKK
                     VFSDIFSKLR"
     gene            complement(22550..23854)
                     /locus_tag="PLU28_00225"
     CDS             complement(22550..23854)
                     /locus_tag="PLU28_00225"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOG33518.1"
                     /translation="MKVKNKIFLGIQLTIMIFTFIIFAFYIIVIYTDSIEKNHEFFKT
                     AVTVFRYWTIEKTSVGSDEMTSLTAENIFPWQELPQELRAFNSMNFFIFDNNGKLLYS
                     KNSVDSELLSKLINLDDQTFKTLKTGGGKIEAYSHKTQESTFAVTVLSSELFQRLYRM
                     ILISIYLFIISAIVSSTVAFMLSRRITKSIYMLINASRKIGCNQYEKISIGGKDEIKE
                     LADTFNSMTEEILKSTSDLEKAVQERTQEIQQKTQELEKLNNKLHMLSVTDDLTGLYN
                     RRFFNNNIDPFFQTCQRNRLIFNIVIFDIDFFKQINDKFGHPNGDKCIKEISYILKKN
                     FTQSNEFIARYGGEEFIVLNMGDSSESFFNHVENIRREIKNRAFCLNGQTVCVSVSAG
                     LVSKIPGLNDSAQEFLSYADNVLYEAKENGRDRTLLLKTDRV"
     gene            complement(23948..24973)
                     /locus_tag="PLU28_00230"
     CDS             complement(23948..24973)
                     /locus_tag="PLU28_00230"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOG33519.1"
                     /translation="MKRSQKLTIMVLILLLVAAINTFVVIIFVKNIEYDINLINFSGI
                     IRASIQRSIKTELNNNPDNFLIESIDSIMKKYSEHPKASLLIKFGEVFHELELDWEKA
                     KELIYDFRRSREPEVRDALFKISEILWSRTNNIVLEAQIISEKKVTEFKAIYIFMIIS
                     LIGIFIIILYIRKYVANELEILAMKDSLTQIYNRSCLDDFLMKELERLRRYKRPFCAI
                     MFDIDNFKSINDTYGHDKGDILLKQISKIVRQNIRISDVFFRLGGDEFMVIFPESNSE
                     QAFTAAEKIRSMVEKTDFFESEKITISIGLAQAKEEDTASSLYKRTDIALYFAKRLGK
                     NRTEISD"
     gene            complement(25150..27117)
                     /gene="feoB"
                     /locus_tag="PLU28_00235"
     CDS             complement(25150..27117)
                     /gene="feoB"
                     /locus_tag="PLU28_00235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HOG33520.1"
                     /translation="MNTVAICGNPNSGKTTLFNALTGSHQQVGNWPGVTVEKKVGKFK
                     AAEKTISIVDLPGTYSLTANSIDERIARDFIIKENPDAVIVVVDQTNLERNLYLVLEI
                     LEMKKKVVLAFNMSDETQRRGISINTENLAKELGVKIVKTSASKKEGLNELKQAIIQT
                     LENGKETQLFSYSEKFMKYLSQTEEILKKHHSDNFVDYGWTALKILEGDTEIKDSVSQ
                     ETMQDIDKLTASMSQEFKEDPSIIVSEEKYKFISSLSKKYVTRKKVNESWSDKVDKVV
                     TNKWLGIPIFLLVMWLTFTLTFTIGDIFNGMLDEGFVALGEWAGAKLGEGMLSSFIVN
                     GIIGGVGSVVVFLPNIFLLFLFISFLGDVGYMPRAAFVMDKLMTKIGLHGKSFIPMIL
                     GFGCSIPAIMSTRTLESKRDRIVTILVNPFMSCAARLPVYTLVAGIFFPQNAGFVIFT
                     LYVLGILISIISALIFKKTLFKNEESVFIMELPPYRLPSIKSILSEAGMRAFMFLKKA
                     GTVIFAAVIVIWLLASLPAGVEYAGEESIIGIFGKIISPIFKPLGFGFWQASVSLFFG
                     FLAKEVVTGTMGTLFGGEEILSQVLPQFFNSVSAYAFLVFTLLYTPCVATIGTIKQEI
                     GAKWALFSVIYSFAAAYVVSFIVFTIGSIIF"
     gene            complement(27114..27350)
                     /locus_tag="PLU28_00240"
     CDS             complement(27114..27350)
                     /locus_tag="PLU28_00240"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HOG33521.1"
                     /translation="MILSECNPGFSGYVKSIMGEGNVKKRLLVMGVTPGSMITVVKKA
                     PFGDPIEITIKNYSLTIRKEEASYITVEKGDEKK"
     gene            complement(27347..27586)
                     /locus_tag="PLU28_00245"
     CDS             complement(27347..27586)
                     /locus_tag="PLU28_00245"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HOG33522.1"
                     /translation="MSLVIAGRGFFKVSKIGGGMILRSRLSKLGINEGSTLEVLSDFS
                     QGPVMLRINGIKYFLGMGTAKKILVSPVSLQEAVK"
     gene            complement(27761..28423)
                     /locus_tag="PLU28_00250"
     CDS             complement(27761..28423)
                     /locus_tag="PLU28_00250"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DtxR family transcriptional regulator"
                     /protein_id="HOG33523.1"
                     /translation="MELSESLENYLVVIYEAHLRNEIARIKDMTASLSVKHTSAIDAI
                     KKLEDLGIVNYEKRGFVKLTQSGIEKAQKIYKNRMTTRDFFINILDINAFEADEFSCK
                     MEHLKSDNLTRSMEMIKSFFEDNPDIKAKYRKYVENYKSKTLETRMCLDQVKPGEKVR
                     VIEILGSEQIHKRLIIMGITTGIEVEVLKVAPLGDPIEIKIRDYSIAIRKSEAKEVLV
                     IK"
     gene            complement(28505..29266)
                     /locus_tag="PLU28_00255"
     CDS             complement(28505..29266)
                     /locus_tag="PLU28_00255"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOG33524.1"
                     /translation="MEQPVLSRTKPQTYKTVVKSIINLTHDIKEINLELISPKEISFL
                     AGQIMSFSVMPDSQGAKTSRTYSISSTPADSGTLQFIVKHVPGGRVSGYIHQKLKQGD
                     EIELKGPFGNLYVRDDGRDMICIAGGSGMSSIKSIVTDLLQKNEKKRQIWFFFGGQTQ
                     KDIFYTEYFGKFEKQFGNFHFIPCIADKNDVSCKCERGLVTEIAHKFIEEKIELPQEL
                     SAYLCGSRGLLEAAVKMLVSSGISEKNIFYDMFNL"
     gene            complement(29350..29964)
                     /locus_tag="PLU28_00260"
     CDS             complement(29350..29964)
                     /locus_tag="PLU28_00260"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HOG33525.1"
                     /translation="MNLDLKKVLKDLYDTKKTEPYFITVPKLKIISCSGYGNPNNSAF
                     FQKTMSTLYSIAYTIKFMLKKTDTSHYTVMPLEGLWWTDEISTFSLEKKEEWKWKVFI
                     VQPEFITSEIFDSGIKELLKKKKEDIDTSLLSFECFEEGLCAQILHIGPYAQEEPTVK
                     KLHEFISLEGYEMTGLHHEVYLGDPRRAAPEKLKTLIRQPVRKI"
     gene            complement(30059..30697)
                     /locus_tag="PLU28_00265"
     CDS             complement(30059..30697)
                     /locus_tag="PLU28_00265"
                     /inference="COORDINATES: protein motif:HMM:TIGR02495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HOG33526.1"
                     /translation="MFAGIRSFSFVDVPDYVSAVLFTQGCNMRCPWCHNHSIAYSDAK
                     EQMSIEQIFKQTELLRKRVDAICITGGEPTIHKDLTKVIEYLKNTLKYYVKLDTNGTN
                     PQMLSELTQKKLIDYCALEIKAAPENYQKLTGTPTDFSKVLQTVDILRKSDLEYELRM
                     TYVPGLSSKYDIDFFKKLLEEKERAYITVAQSTDIYTVSSMKELIPKRFILR"
     gene            complement(30682..>31795)
                     /gene="nrdD"
                     /locus_tag="PLU28_00270"
     CDS             complement(30682..>31795)
                     /gene="nrdD"
                     /locus_tag="PLU28_00270"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HOG33527.1"
                     /translation="KYGTPYFANYISSDISPQDVRSMCCRLRLDNTVVMNHIGKLSQS
                     FNISNKSYENDTSIRRGGLFSSNPLTGSIGVVTINIPRIAMMSHNDFKKFYTLLKDAM
                     NTAKESLEIKRRYIEELTVSGMFPYCRVYLDKIYALSGQYWANHFSTIGLVGMHEGLL
                     NMGIKDGILNRQGTEKALEIMDFMLDTLKEFTNETNNLYNLEASPAEGTSYRLACIDR
                     KEYGNSAFFSGTEETPYYTNSVHPPVNSFDDPFDLLEHQKELQTKWTGGTVVHIFVGE
                     KISSANVLKDFIKKAFTNYRLPYMSITPTFSICPVHGYISGEKFVCPICSRETEVYSR
                     IVGYYRPVKVWNAGKKQEYLHRVQFSLRSDSKCLQE"
BASE COUNT         9626 a         6025 c         6127 g        10017 t
ORIGIN      
        1 ccgatactgc acgcaaagag ataagcagct accttgaagg cagtgcatca agcgctgcaa
       61 tagcagtaag gatagacggt cagacagttt attcagaaag ttttggagtt tgtgatctaa
      121 acacgctcag accggtggaa agagacactc agttcaatat cggatcagta agcaaagtat
      181 ttgctgctgc atctgttctt attcttgtgc agcagggaaa ggtaaacctt gatctgcccg
      241 ttttcattta tctacctgag ttttctatga aagatcctag atataaagat attacagtac
      301 ggatgctttt gaaccacact tccggtattc cagggacttc aatcaaagat agcatgactg
      361 cgcaaaaaaa tacagggtat atacagcagg tcattttaaa ccttaaaaat agctatttaa
      421 aacacgatcc tggcaagata agcatctatt gcaatgatgg ctttactttg gctcaggctc
      481 tgattgagaa agtttcaaag acaagctact ccgatttttt aacagaaaac atattctcca
      541 aattggatat gagtaactca acaacatatt ttaaggagaa caatgagaat attgcacgat
      601 actatgacca tgaccagaat atttcttttc ctgcagaata tgtaaattca ttgacttccg
      661 gcgggcttgc cagtacagcc gaagatcttg taaagtatgc tgagatactt tataacgata
      721 aaattttgaa tgaacagctt cgtaaagagt ttatgagtcc acaggctgga tctaaaacgg
      781 ctctaagcgg tgaacctctg tatatgttcg gtctggggtg ggacagtgtt gaagattccg
      841 tatttcatag tagtggtgta actgttctta ccaaaagtgg aggaacctgc ttttattcag
      901 cacagcttca tgtagtgccg gagtataaga tctctgttgc tcttaccgtt tcaggcagag
      961 tagatgtaaa aagtataaat gatgttgtgt tgaccgaggt acttaagcaa aaagggatct
     1021 tagcaggatc aaagaaagtt tttaatatac agactgtact tccacttagc ttctttaacg
     1081 gctatgaggg tatatacgca tccctggaaa gcatctttaa gtatgagata gatttacaga
     1141 ataacggtat gaatgtggaa atttttaatg gaagcggttt tgttccttac gattttctca
     1201 gatacgaaga cggctatttc atttctcgca gttcagatgt ttattcattt ataagcgatg
     1261 actatggtaa gtatatccag cagcatttta atgacagaaa gagtacaaga gttcttattg
     1321 aaaagataaa gccttcagaa aacatagatg tcaaagagtt tgaaaataag gtatggattc
     1381 ctctcaactt aaaaacacag gatctttgtg ttatgatggc acggacaggt tctgtcggcc
     1441 aggctgaagg atatgcatac ctgcagttct ccggatttca tatgaatgta ttgaaggatg
     1501 tttttacagg tgagaataat ctcaggtaca taagagatac cgttgctttt agaattgaaa
     1561 agacagccgg ctcatgcaat cttttgtttg aaggttacag atacgctgat gcaagtaatt
     1621 tgcctgttct tgacaggaat gaagttatta aaataggtct ttcaggagaa aatatttcaa
     1681 ggagagtatc ggacgatctt cttttgaagt ttgatattca agatggagcc agagcggtta
     1741 ttttttccga cagtcttgag atgatctacg acagtctgat acatgattac cgcgaggtgt
     1801 atgtaccgaa aggctcttat gtgactttca tatgtgagcc tggagcccaa gtcggtataa
     1861 accttaaaaa gtatcagcag gatgacagat acagacaagt aaaggcaaga gcagaaaaga
     1921 ttgccgatac gcttatagat aattatggag ttacaagcct tcagtatgct cttatataca
     1981 ataatgaact tatactttct tcctttgcgg ccggaacaga ctctgaaaaa gcagaggagc
     2041 taagtacatc tacaccatac ggaatcggtt caaccagtaa gatgtttact acagctgcca
     2101 ttatgtatct tgtagataaa ggacttgttg aacttgataa acctgtttcg gagtacattc
     2161 cacaattcac tatgaaagac gaacgataca aaaaaataac tgttcgcatg cttctaaatc
     2221 actcatcggg attaatggga tctatttatg cgcagggacc tacctataat catccggata
     2281 caactaatca cgataatata ttaaaggaac tatccgggca gatgctgaaa gctgaccccg
     2341 gagcattttc agtatactgt aacgatggat ttacgctagc ggagctggta gtggaaaaag
     2401 taagcaaaat gagttttaca gagtttataa gagtttttat aataaatcct ttaggaatga
     2461 aaaatacgtt tacaccgcag gatagctttg aaaaaagcgg acttgccgct acttttatga
     2521 atggagtcag gactcctagc gaaactttca atatgatcgg tacaggaggt atgtactcaa
     2581 ctgctgaaga cctttgcaga ttaggccaga tattcatgga tgattccgga tacagtcctg
     2641 ccggacttat cctctcgcgg aaagccaagg atgaaacttt ttctaaagag tacagcaaag
     2701 gagtatggcc cgttcagact gataacattg caggcttcgg attgggctgg gacagcgtgg
     2761 atctatatcc ttacagcagc tattcgatac gtgctgtttg caagggtggg gatacagggc
     2821 tttatcacag cagtcttgtg atacttcccg agtacaatat agcttttgcc gcaacatttt
     2881 ccgcgggttc aagtacttta ggaacagtat ttgcacagga aataatcctt gatcttcttc
     2941 aagctgaagg aaagattgac cgttcatcgg agaaagataa gaaaagtcct gctgtattgg
     3001 ccgatatgcc tctttatatg agtgagttca gcggaagcta cgagaacaat gaaaatatat
     3061 acgatatcca gataagcaag aatggagtcc ttactgaaag aacaatctat ccaaagggtt
     3121 tgccagaaca tgagtacaag tatacaaagg aaggcctttt cgtcaatgat gccggaaata
     3181 agaagctttc ttttgtaaaa gaaagcaacg gtataactta tatatgtaca gaaaagaata
     3241 tggttcttaa cggtctggga agggtaaaag taaaaaccta ttcggcacag aaaacacatt
     3301 tttcagatgt ggcagacggt gtcaaaaaag tatgggaaga aagaaccaag aacaggtact
     3361 ttcttgttaa tgaacaaagt acaagccaga actatcatca attggctgca gcctctatgt
     3421 ctttttcaat ggatagtgaa cttaagggtt gtatttcaaa ctaccgtatc ataaacaata
     3481 attttgctgt tgtaaatgtt cagattcccg gatttgacgg tcgtgatact cagagcatcg
     3541 aagtatatga aaaagacggc aaagagtata tcaaaaacgc aggctggatc tttatatgtg
     3601 ataagaatat agagtatctt aatgggtcgg agacgggttc atgcgttatc ggcacggacg
     3661 ggtatgcctg ctggtacaaa attccggatg aaaaagcagg gaagaccatt gaagtaaaaa
     3721 aacaggaagg ttcttctttt gcggtatatg atgaacagga atgtgttttt tattcggttg
     3781 cttgcggaaa caagccggtt atacttccca agaatggaat gattttattt gcgggtgaaa
     3841 agcccgggga tgttctttac tattcaataa aatgagaatg agctgggtgg tggaaagcat
     3901 gaaagaagga tttattgaga ctaaaaacgg taaagtatgg tactgctcct tttcatcggc
     3961 aaaacaaacg gttccgatat tggtagtcca tggggggcca ggatttgtaa gtatgccgca
     4021 ggtgattgag gagttctcag atgatcacga tgtttacttc tatgatcaga taggaagcgg
     4081 aaagtctgat agagcaagct gtaatgattt ttacagtgta gaaaactttg tagacgaact
     4141 ggaagaagtt ataaaagcat tgaaatttga acaggtaatt cttatgggtt tctcatgggg
     4201 ctgtgctctt atatgtaaat atatgttatg caaaaaacct gaaaatgtaa aaggaataat
     4261 cctttctgcg ccgtatttga gttctccgag atggtatgct gaccagagag aaaatattat
     4321 gaatatgcca gaggatacga gaaaagctat tctgcttgga gaagagaccc aaaactacgg
     4381 agataattat cagaaagcga tgatggagta ctataaaaag catgtatgtt gtcttaatcc
     4441 gtggcctgac tctatacagc aggcttttgg aaagcttaat atggatgttt atcttactat
     4501 gtggggaccc agtgagttta ctattacggg caaactcaaa gattttgatc ttctgccgga
     4561 gcttgaaagg atagatgttc ctgtactcct gacttgtgga gattacgatg aggcagagat
     4621 aaaaacagtc agagattatc aaagagcttt taagaatgca agtatggctg ttatacctat
     4681 ggcctcgcat cttcaccaaa tagaacagcc tgaaatatat aaaacggttg tacgggcatt
     4741 ccttaaaaaa atttgtgatc ctgcggcgct gaagacatga ttacttttaa agaggatgtt
     4801 tttaaaattg atactgactc aagcagctat gtgtttgggt taaatcatgg aatgcttgaa
     4861 agcatgtatt atggaaagaa gataagggat gggtctgatt attatgcact tagggaaaag
     4921 tcgtcgggag gatatcccaa cacggtttta actgaaaacg atgataagac ctttgtaatg
     4981 gacaatctgt gtcttgaata ctcatttccg ggaaaaggtg atttcagaaa tacagcgatt
     5041 gagatcagga tgcccgacag ttcttttgtc tgtgattttc ggtactcttc acacaagatc
     5101 tatgacggcg gctataaaat accgggtctc ccctttgcaa taaaaggcga tcagacactt
     5161 gaagtcaatc tgatagattt gagtgggctt gagcttatac tgtactatac agttttttct
     5221 aaaactgacg ttattgcaag aagcgtttgt attaaaaacg gagcggataa ggatatatat
     5281 ataagaaaaa tcatgagtat gcagcttgat ctttcagaca gcttcaagct cctcacattt
     5341 aacggtcttt gggcaaggga aaggcataaa gacgaaaaaa atctttccta tggagaagtg
     5401 gtttgcagtt ctgtcacagg agcgtcttca aacaggcaga gtccttttat ggttcttgcg
     5461 gaccgtgagt gtacacagag ttcaggctgc tgtatagggt acaatcttat atacagttca
     5521 aaccactatg aatctgttca ggtaagcgga tacgataaaa cgagattcat gggaggcata
     5581 aatccggctt tctttgaatg gagggtcgga cctaacgata ccttttttac tccccaagct
     5641 gtaatctctt tcagcagctg tgggttaaac ggtctgtccc aaaatatgca cggatttgtc
     5701 agggagaaca ttatacgggg tgaatgggca aaaaaagaaa gacctgttct tataaacaac
     5761 tgggaagcga catactttaa ctttgatgag aagaaactac ttgaaattgc caaggtttca
     5821 aaagatcttg ggatagagct tttggttctt gatgacggtt ggtttggctt acgcaacgac
     5881 gatactacat ctctgggaga ttggtatgaa aacagaaaaa aacttcccgg tggactatcc
     5941 tcgcttgctg aaaaaataaa tgagatggga atgatgtttg gcctttggtt tgagcctgaa
     6001 atggtcagcc cgatcagcga tctttacgct atacatcctg aatgggcggt aaaagttcct
     6061 tcacgaaagc cttctcttgg aaggaatcag cttgttcttg atcttacaag aaaagatgtg
     6121 cgtgattata tagtcaatgc agtcagcaat attcttaata gtgcaaatat ccagtatgta
     6181 aagtgggata tgaacagaca tataacagat gcttattctc aggaactcgg agccaggcag
     6241 ggagagtttt accatagata tatcttgggg ctttacgata ttcttgaaag agtctgtggt
     6301 gcctttccgc atgtgctttt tgaatcatgt gccagcggtg gagacaggtt tgatctgggt
     6361 atgctgtgct atatgccgca gatatggtgt tctgataata cagactacta tgaaagagta
     6421 cttattcagg aaggtacatc ctatggtttc cccttgtgca ctatgggagc acatgtatcg
     6481 gctgttccca atcaccaaac catgcgtaca actccattgg aatcaagatt taatgtagcc
     6541 tgtttcggac ttttaggata tgagcttgat cctacaaagc tttcagataa tgaaaagctt
     6601 gcagtaaaag aacagataaa gttttataaa aagtatcgta aactctttca attcggagat
     6661 tttttcagga taaaggatgg gtttgaatct gaacatgcag tctgggaggt ggtttctgcc
     6721 gataaaaagc agagtatctc cggttttttc cagaagcttt ttgttcctaa tccaccttct
     6781 gataagctgt tttttaaagg gcttgatgaa gagctgtttt acaaaatttc atgcagggaa
     6841 cagtatttgg agtttgttcc cggcagattg tctgatgatg aaggttttct ctcaaacaaa
     6901 gaagagtatg gaggatacgg cgatcttctt atgaatggtg ggataaagct aaatcagcag
     6961 tacccttcaa taaggtacaa tcaagggagc agacttatgg gtgattttgg ttccagatta
     7021 tatattgccc gtagtgagga ggaaaaatga aaatactttc gataataggt gccagtactt
     7081 ttggaaatac aacagaagtt ttgagatact ttaagagcca gcttgaaaaa tgcggcggtt
     7141 atgagtttga agatctttac ctgcaagatc ataagataga tttctgtaca ggctgtcaca
     7201 gctgtataat gcttggacaa gagaaatgtc cgcattatct tgcggtaaaa gagattgagg
     7261 acaagatgat gcaggcagac ggagttatcc ttgcaactcc tggttatatg ttcagtgtaa
     7321 gcggcataat gaagaacttt cttgatcatg ttgcatataa ctgccatagg cctaagtact
     7381 tcggaaaaaa gatcttcctt atttcaagct gtaccaaatg gcaagaggaa ggagttttca
     7441 ttcctatgaa aacatgggct cagggagcag gatttgatct ttgcggcagc atgtatatag
     7501 atatgatgcc ttttccatac agtgaaggcg aacttgaaaa aagaagaaac aaagtaaatg
     7561 aagcggcttt ctcatttgat aagattatca aagaaaaaaa ggaaataaaa cccgaatttt
     7621 caaggataat gatttttcat gccttcagga caatgtcagc aatgtttccc aaagtaatgc
     7681 aagcggatta caggtatttc accagtaaaa acgcatacga taaaaaaacc aaatggtaca
     7741 taccggctaa aatttcattt ttaaaaaatt ttatagccgg tatgattgaa aaaaagatga
     7801 aaagctctat aatggatatg atcgacaaag agaaagcgga aaaagcagaa ggcgtgttcc
     7861 gaaataagct ttaaatactc tcttttaccg gagttttttt gtttaaaagc tcgctggcta
     7921 taaacatgca ggcaataagt acaagcaacg ctaaaggcat aagttcaaga gttattttag
     7981 aaaaaagtac tcccagacct gctgtaataa acgaacctcc tatacatgcc gcaccgacct
     8041 gataacctat gagagatctt gatgtgccag catcaaatct tttgggagtt tcgtgcatca
     8101 tgcatgggta tatcggagca aatccgagtc cgcacattat aagtcctatc atggtgagag
     8161 cagggcatgt cctgaaaaaa atcagaacga ttccaaaggc acttagtata agcccggcct
     8221 ttatcataaa acggtttccg agtttgttta ctacgaatcc ggttaaaaat cttccaagca
     8281 ttatcgagcc gtagtaaaac gatatccaaa gtcccgcagt ttctcccgtg aggccgcgtg
     8341 attcaataag catactgcct gcccaaaggc caacagtata ttcagcacct gtatacacaa
     8401 aaaacatgaa tattgaaagc catggcgaaa tatctgtcaa aaactttttt ttagtttcat
     8461 gagaagacgg tacatcctcc ttttcttttt ttgtactttc ctgccatagg cctaagctta
     8521 taaagaaaag tacagcaaga agaagctgta ttaagcagat gcttagataa cctttccgcc
     8581 atgttcctcc gcctctcatg accgcagata gtataagcgg tccggacatg gcgccgactc
     8641 cccagaagca gtgaagccag ctcatgtgtc ttgacgagta gtgatttgcc acgtagttgt
     8701 taaggcttga atctactgcc ccctggccaa acccaagagg aaatgccaga aaaatcaacc
     8761 aggcaaagga tgggataaat gagtagccta gaagtgccga tccggtcata atgcagctaa
     8821 taagcacaag ttttcctgtt cctgttttcc gtactatcct tccgttcatg aagcttgaca
     8881 gggcagaaca aagcattaat ataaccgata ttataccggc tgcttcgaga ggctgaccca
     8941 aatcaattct tatgctgggc caggctactc caaagatgcc atcagggagt cccagactta
     9001 taaatgccat atatactact attaaaagaa aaagattttt cattgtatca ccttgttatt
     9061 aataaattaa tatcccagtt ttttaagata atccgtaaat ttttctaaag aaaaatattt
     9121 ttcattgcat cattttgttg ttaataaatt aatatcccag ttttttaaga taatccgtaa
     9181 atttttctaa agaaaaatat ttttcattgc atcattttgt tgttaataaa ttaatatccc
     9241 agttttttaa gataatccgt aaatttttct tctgtcataa gttcgttggt gataaacttt
     9301 cccttgtcat acaaagagaa gatgccataa agagctccgg tctgtcttgc atgctccgga
     9361 cctgacagtt ttatggtttt aacggtaact cccattttct gagctgtctt tgccattatt
     9421 tgggagtaaa caggagcaaa tggacacata tcggaataga caaaggtcat tgcctgagat
     9481 tcttctatgg ttccctgttt tgcattctct gtaaacttag ggtcagctgc ttgcggtatg
     9541 aacttcttta cgagtagctc aaagtcacct gctgcactgt cacagacaga aaaacctttt
     9601 gaaagcatat attttttatc tgatacaaaa ggttttttct tttttgaaga aattattacc
     9661 aatccattgg tatttaaaga atctttctca cactcttcta aaagcttaga ccccattcct
     9721 gttcccttaa actttccact aacccaaaag cactgtataa aggtataacc gtcagcattg
     9781 ataggaaacc aagcttgttc ggcaggacag tactctatga aacattttcc ccgcacatca
     9841 aacttcttga aaacatgccc ttttgaaaat tcttttttga gccattcttt tttcatactg
     9901 gcacgcgaaa ggttttcagc atcgttgcca atagcacaac atatatgctc actatctatg
     9961 ttttgttcgt ttaaagttat aattttttca tcctgcataa aatccctcct tttgtataat
    10021 tataacacct gcggcctgac ttaatatgtc aggccgcagg ataaagggac ttttgaaatg
    10081 tatctgtatc ctatcttatt ttttgagttc cagataaaga ttctctatct cgtcaactat
    10141 tcttggcgaa gggcgaagta tcttggaaga gtctataagg aatattttat ctttctttgc
    10201 tgctgaagta ctttttatga gaggacttgc atcctttatc tgttggggtg aggttatgct
    10261 catagcgcct attataaagt ccggattctg ctgaagaata tattcggcac tcagtatagg
    10321 cttatctgcg ctcaggtcgg cggcaatatt tttaactcca ataacttcaa ggatctgtcc
    10381 tgaaagtgaa gtactgttaa atgccattat aggattagtt gagtaaagga aaattccctt
    10441 catgttcaat ggattcttta aaatatcagc ttttattttt tttaacttac ttttcttctc
    10501 ctgtgaaagg gtgatagctt ctttttcttt gccgcatagc actccaagta tttcaagatt
    10561 gtcaagaata tcttcaacag aatctgcatt aacaatcaga taatttatag agtgttgctt
    10621 gagggaaagt ccaaaatcac ctatcatagc atttaaaaga acaagatcag gagtatagga
    10681 aagaaccttt tccagagatg gcttggttat acttccgaca ctggcaagag caggtgtctt
    10741 atcgtaaggc catatgggtg atttagcagt gtgacctata gctgctatac tttcctgcgc
    10801 tcccagaaga tacagaactt ccacggatga aggatcaata accacgatct ttttatatga
    10861 agcaacaggc acgctctctc ccgactctcc gtatatcata ctgttttcca tatgtagtga
    10921 aaaagcagac agccctataa tcagaatacc taataccagt atagcttttc ttatcatgat
    10981 tcctcctttt atattgaccg gaaattaaag aatacccgtt tcggaataat tctttatgtc
    11041 cgctgttttt acagtatttt ctattgctga tttcattatt tgtgctgcga tattattacc
    11101 ccagaaaatc ccgtcttcgt ttagtttgaa tattccctta tccgactgaa taaaaaagcc
    11161 ttcttccatg taggtttcaa ttttttcaca tacagcgttg taagcatctt cgttgagata
    11221 atttcttaga aattcatctt cataggttcc gtactgaaag tagcctaaaa gcctgttaaa
    11281 aaaagcataa acaggatgat aagtcacaat catttttctt ccgggagcca tgttgaatat
    11341 accgtatctt ccgatacttc cgccagcacc tacacctacg ggaactgtat cggcattggc
    11401 gtatctcatt tttatgtacc tatatctgtc acgtccgggt ttggccagtt ttgacagctc
    11461 aagaagatcg tatccgtttt ccgtcatctt cctgaaaaaa gtattatgat tatgtttatc
    11521 ctgttcgatg tttctttcaa aaatgatctt tccttcagtt atgctcttat aaagtgctga
    11581 tccgttgtgt atcataagag agtagaagct cacactatcg gcatcgacac tgccacaaag
    11641 caatgcatcg ttttccactt caccgatact ctcatatgga tatgagtaga ttatgtccaa
    11701 acatacatta cctttaaaac tctttttgat ctctttaatt ctgttaaccg catactccgg
    11761 tgtatacatc ctgtttagaa gcttgcgtcc gctggaagaa aaggtttgaa ttcctatact
    11821 gaatctattt actccgtgtt tagacatcac ctgtattttc tcgtccgaaa gattgtgcag
    11881 agttgtttca aaagtaaact cacagtcatc agaaagatta agcactgtat ttatagtgca
    11941 taaaattttt tcaagatcct gaggttcaaa tgtagtagga gtaccacctc caaaatatac
    12001 tgccgaaaaa acagcgtttt taatgtactt tttctggctc agaatattaa gttcgtttac
    12061 tacatactgt gcatatccgc tgagactgtc cttcttaagc tctctgttca gattacagaa
    12121 ggtgcagagt ctgtcgcaat aaggcacatg aatatataaa acctttggct ttgaatcctg
    12181 attgttacat aaaaaatcat caagttttga tatgtcggaa tgttcttttg gaaagtccat
    12241 tcctaagaga tcatccacaa aacgttgaga gtcgtggtga gatcttagtc tttcttcaaa
    12301 cataagccat ctccttttct ttttttgctg atatggcggg atgagtttta ggtacgataa
    12361 aaggaatatc ttcgtctgtt tttagtattg tggcatctat accgtaaatg tctttgatga
    12421 tttcacgggt atatacctgc tgtggtgttc catgatatcg tactgagcca tctttcatga
    12481 aaaaaatctc agaacagaac atagccgtca tgttaagatc atgaagaact gcaagtgcgg
    12541 ttagttcctt tttagccgta agttctttta ctatagccat tatttccata cagtatttca
    12601 tatcaagact cgaagtaggt tcatcaagaa gcagaatctc cggttcctgc gcaagcgccc
    12661 ttgcaaggag aacacgttga aactcacctc cggaaaggct aagggcagat ctgtctgcga
    12721 atttttcaag gttgagcttt tttattagat gttcagcaat ctctttatca tgtttggcaa
    12781 agccaaccca cttgtttttt atatgaacca cccgtcccat cattataaga tccatgacgg
    12841 tcaaatctgc cgaaagagaa gatttttgag gtataaaact gacaatcttg gccatttcag
    12901 cttgtttcat tcctttaatg ttgatccccc gtatttctat acttcctttc tgcggagtat
    12961 aaaagttaag tatgtttcgt atgaaggtag ttttccgcag ccgttaggcc ccattacagc
    13021 gctaaaagaa tgcttttgaa tagatacgtt catattgtca agaatattga tctttccgcc
    13081 gtaagcaaaa caaagttctt ttgttgatat atcccacatt atctgttcat acctcctttt
    13141 ttattaaacg ccagatatag aaaaaaaggc gctccgaaaa atgaagttat tattccgatc
    13201 gggatctcca ttggtgcaag tatcaaacga gatatggtat cacatgccaa cagaaaaagg
    13261 cctcccgtta tggttgaaag tggaatcagt tttttatttg aacatccgaa taaaagtctc
    13321 accgtgtggg gaacaattaa acctacaaag cctatcatgc cggaaaaggc aacagaaaaa
    13381 gctaccacta gtgctgttat tacaagcata gattttttca gtctggatac atttactccc
    13441 attgaatgag cctcttcttc gcctgacata agcaagtcca gatcgttgcg gaaataaaga
    13501 aaaactgtta ttgaaaatac cagaggaaca atcataaaca gaatcttgga ccacgttgcc
    13561 cctccaagat atcccatggt ccacattatt attttgaagg agtcctctcc gatcatgtac
    13621 atggcaaaag aagtaaaagc gccaagaaaa gatgagactg caataccaac tatcagaagg
    13681 gttgccgtgt tcagcttttt ccctgcatta gatagtctga atatcaagaa cgatgtaata
    13741 acacagctta caaacgcaaa aactccatac atcaggtcag gaaccttaag aataaatgca
    13801 agaactgcac cgaatgtggc acttgcactt atgcctatta tgtaaggatc tgccagggga
    13861 ttttggtata ccgtctggac tactgttccg gttgaagcta gcatcattcc tacaagaaga
    13921 gccataacta tacgcggaag acgtatatcc atgattatag ttcttatgta atccggggtg
    13981 tttgccgatc tgttgaataa aacatccata atatccctca aagaagtttt tactgctccg
    14041 ttcattagag aaaaaattat caaaaaaact atgataaaga aaataagaaa aagaagtatc
    14101 cgctttcgca ttttgttctc ctcgacaaaa aaacattttg tatacttgcc tacattatat
    14161 caaagaactt gttttttgtc aatatcagta taaactatat aaatactata tatagtaaga
    14221 aaaaatataa tctctcaaaa aatataaagt agttaagact acaaaaaaag aacttatcca
    14281 gcattttcag catgtgataa gttctcttga aactaagtaa aagttttcag ttataagcca
    14341 ccattttttt taaggttttc atttgcatct gcaatcctat tagtatcatg ataagatcaa
    14401 caagcggctg agtaaaccac actgcgctaa taccaaacat gttagggagt acaagcatgc
    14461 acggtacaaa aaggatcagt tgcctgaaaa gtacgataat gcctgctttt ttcccgtcgc
    14521 caatggattg aaaaaaagtt attgacatga ccattattcc ataaaggata aaaacactgt
    14581 aaaaaagcct aaaattgaaa ataccttcat ttatgatgct ttgactgaca ttgaatattc
    14641 tgagtactat attcggaaaa agctgtgcgg gtatccaaaa tatagctgcc aaacaagctc
    14701 cgccaatcat aaagaccttc atggtctctt taactcttag gtattttttt gctccgaaat
    14761 tagtacctat tataggctgc agtccttggc tcattcccca taatggaatg aatgaaaacc
    14821 cgtagaatct aagacttgct gccattaata ttccgttcga ttctccgccg tacttgaaag
    14881 ccattttgta aagcatggtc tgctgaagca taaacaaaag ctgcataagc attgcggagc
    14941 ttcctacaga aaacatttct tttgatatgg atttttctgt ttttatcctg tgaattcgta
    15001 cccgtgggct tttttttata aagtagtaaa tagttgcaat agcctgaaga atttgtgcgg
    15061 agacagttgc aagtgccgca ccttctatgg ctttttcacc catgactgtc ataagtatag
    15121 gatcaaggat tatatttatt gcggctccca agcccattat aatcattgct tttttcatca
    15181 gaccttctcc gcgcataacc atattggcac tttgagtaaa attaacaaat aaagaaccca
    15241 ggatggttac tttcagatat ctgagcccca tttcagctat ctcaggtgta gctcccacaa
    15301 ttttaagaaa ggtttcagcg aaaacaagaa caaataccgt aattgctatt gaaaaaatcg
    15361 ttatccatgc aatcaggttt cccataatct tatctacggt gttttggtca ttttttccca
    15421 tggcgcgtga caagatagaa gccgaaccta tgcctatgag agtagcaaca ccgttattaa
    15481 gaagcgttaa agggcttgca atgcttatag cgctcatagc tctttctcca attatatttc
    15541 cggcaaatat tccgtccatc aaaggataga gacctataac caccattcct atgactgccg
    15601 gtacagacat ctgaaacatt aaatctttag gtttgtttga tagaagctgt tcttttatag
    15661 acatgctatc gtcatctcca gttagtattt gaactaaaaa ttaagttatt ctctcagaaa
    15721 gtcaccgttt ctaagagctt ttcttttttt ttctataaac tcatcaatct cagtcagcct
    15781 tgaaagagtc tgtttttcaa aaggagtggt tttgatgact ttttcgatac gaccattttt
    15841 cattactgcc cttttatctg ctgatagagc tataagggga tcgtgagttg caattatgat
    15901 aatttttttg tttgaggaaa gaagtctgag tgcttttttt ctatcaatgc ccgcattttc
    15961 aatttcatct ataagaatga ccggtgatct gctcagaaag gcaatatctg cgaccataag
    16021 tgctctggcc tgtcctccgc tcagcgaagt aagaggtgaa gaaagatcaa acggctctcc
    16081 tgaaagttca ttagcacttt gaataatatt tttggttttt tccgctgttt ttttatctat
    16141 aagactttgg gcatgaagct ctataaactc tttgacacat acatccagga caaaattcat
    16201 attttgagat aactgggcaa caggtttgtt ttctgcggaa aatctcatag aatcgggaaa
    16261 aggacgccca tttaccaata tttttctttt agtaggcgta tctgcttgtg ccaaccattc
    16321 aatatcagct aacaatctgc ttttgccaga acctgtattt ccagctatgc acaaaacttc
    16381 tcctatacca acagtgaggc ttatgttttc gacagttcca tccttggcag ttcctcccac
    16441 gatagttact gtctttatat caggcatgcc aatgctgaac atgttttgca tagtatctat
    16501 gaaaaggtca aggcgttttt taagctcatc cctgcttgca cctatatcct gtaaaacaga
    16561 gtagggtaaa gatgaaaaat actcatggac agatacttct tcgcctgcct gaggaagacc
    16621 attaatatta aaaaactcac ttgaatatgg ataaacgtta agcatttcat gtactttcat
    16681 actttttatt ttgttataat ttttcactta cagcttctcc tttaattgaa tcaaagttta
    16741 ttcttcttac attgcctttt tgcttatctt tcccaataaa agtttctccc atacagtaag
    16801 agcataaagc gcttggcatg cttgcacgta gctcttttcc ttcaagagag tcaatttgtg
    16861 gagttttttc aagtaaatca gacagtctgt tcgcaccttg ccctgtaatt ccgtttatat
    16921 gtagtattat tgcattagga ttagcctgac gtattttaaa ggcaaatatt tctttttctg
    16981 cctgcgatac aatttcgctt ttagtaatca cagtgatatc ggatgtttta agcattgggc
    17041 ctatctttct tggagtattt attcctaata ggttatcaat tacacataca gcggtgcatc
    17101 cttctatatg tggtgaacat cggttgcata gccctgcgct ttcacttata agaatatcgt
    17161 aaggttttgc gtatgcccag ttaaagcact cgtcaatgtt gcttataaaa aaatgatcag
    17221 ggcatagatt tccggataat cctgtaatgc tttcaatccc attctttgca tataatttgt
    17281 catcatctgt tgccaaacag tcaaacttta ctactgcaat tttatatttt ttctttttaa
    17341 gttctaaagc tgttttgatt atgacagaag tttttccgga cgatggcgga ccggagaatg
    17401 taaaaaatct tatatctatc tcctccttat attttttctt cctttctttt gcggtaaagc
    17461 aggaagataa agaaaggagc cccaagaagg gacgtaatta ttcctacgga aatttccaca
    17521 ggctgaaata atgttctggc aactgtatca gacagaataa gcaaagcagc tccgataagc
    17581 gcagaaaggg gaaccagaaa gcggtgatcg tttccgacta ttattcttgc acagtgcgga
    17641 gcaataagac ctacaaatcc tatagtgcct gctgcactta cggcacttcc ggcaagaata
    17701 gaagatgtaa gcagaataat agttctgtaa aaacgtgttg aaagtcctac actgctggca
    17761 acctcttcgc ctaagccaaa aagattaatt tttgggctta taaatataga aactgtcaaa
    17821 gcaataaggc tgtaaggcca gatagaatta aatgccgtcc atcctcttgc actgagactt
    17881 cccaagagcc atgagtacgt tatttcaagg gtatcactgt tcaggctcat tagagctgaa
    17941 gttaatgcat ttaaaaggtt tgataccgct ataccggcta aaataacata aacggtacgt
    18001 gagttctgtg ttgaatatga aagaagatag ataagcatcg atgctgctaa ggctcctatg
    18061 aaagatgccg gcgggataag cgacacattt cccgggaata tagtcataat aataactgct
    18121 gccagacccg ctcctgaatt aactcctatt atattgggcg aagccatggg attacgtaaa
    18181 actccctgaa gcaaaacacc ggcaactgaa aggttcattc ccacaaaaag tcctgtaagt
    18241 attctgggga gccttatatt tacaagtatc tgttttgcca tactctctga agaattttta
    18301 ccaaaaattt caaggatatc ctgaaaagaa aactttatgg ttcctgtaaa aagactgaac
    18361 aaaataatta aaaaaagtac gactgctact gccacaataa ttagcaaacg atatctgtta
    18421 ctatagaaaa aaaattctga gtttttcaaa gatacttctc ctttatgcaa ctgaaaaaat
    18481 taaaaactat ttattaaacg ctttttcatt gttcaaaatg acttcagctg tttttgaaag
    18541 catggcttca ggcagtgaac ggtttggatt tatagaaaat ggcagaataa gaattctgtt
    18601 attctgaaca gcctttaaat tactccattt tgaatctttt ttatatcttt caagcagctt
    18661 tcgattattt tcctgattgt tacttggagc tactataaaa ataatatcag gattgctttt
    18721 gattatggct tcaatgtcaa aagccacaaa agatgatttt gaagcgttgg gtaaaccatc
    18781 cggaacaata ttttctattc ccagaagcga taacatggat ccgtaggaag ttgcagactg
    18841 tgcggcggtt atcgtatctg tagcctgtag aacaacaccg gtttttatat tggttgttgc
    18901 caattgtgat ttaagctttt cagctgtttc aaataccgaa agaacatatt tgtaagcaag
    18961 ctcctccctg tcaagaagtg caccaaaata cgttgacatg cctatcaaag gtatatctcc
    19021 aatattttcc gggtcaaagt aaaaaattac tgctccgcac tgtgcgagtt ccttttcaag
    19081 attagcgtgg aatctgctgc ttgcaagtat aagatcaggt ttcaaactat atacagcttc
    19141 aatatttata ctctttgaca taccaacacc gggaagcttt tttccttctt cagtgattgt
    19201 ataatcgtca acctttccta ccggctcaat attgatgctg taaagcgcct gcattgccgc
    19261 tgaagtaaga gctaaaattc tttttggatt agcctgaata gtaacctctc tcccaagaca
    19321 gtctttgaaa accgttttta ctatttgggg tttattttga agatcacgaa cggtatctgt
    19381 tgaagaaaaa cctgtgattg ctgtgcctgt aagggctata gcaagacaaa caagcagaac
    19441 tgttttttta gaaaaaaatt tgaacatata catcctcctt aaagatattt ttatttattt
    19501 agttcaaccc attttccact tttcacatct ccatcagtga aaactctgaa ctttttgagt
    19561 cctgaagcag caaccataag tttgaattca tcaagagaag gtcttttttc atttttcttt
    19621 tcgagttctt tgtgcataga ggttaaaagt cttttgcgca tcgtttcagg tgcatagcga
    19681 cccaatccac ctccaataac tgcgtagcag cccggtttta acacccgttc aatttctagc
    19741 agtcccttac aagaatcctt ccagaaccat attgatcctc tgctcattat aaaatcagca
    19801 taattatccg ggaaagggag attttcaacg tttgcggtaa caaactgtgc gccattttca
    19861 gtacctgact gaataaaatt tttatttgcc agagcaatcg catcagaatc tatgtctgta
    19921 aagattattt ccattttggt tatttttgca agttctattc cgaggaaacc cggaccagtt
    19981 cctatatcta tggccaggcc tgtatcaaga ttgtaatcat caacaaactg ttgtatcaga
    20041 aggggatata tgggtgcaag catgtcaaaa tttgatttaa acatcttttt tctcctttgg
    20101 gctacagtca taattatcaa agtctgagac ttctcttaag aatacatccc atcccttcca
    20161 gtttttcaga atagctccgt acaactctga tccgctcata cgcaaagtat ccttacagct
    20221 ccatacttcc tgatcattca gatagcggtg aaaatcttcg gtcataaaca ggtcggcaat
    20281 aaaatcaagt ttttctgaac tttctttttt ccaagcaaaa acctgcggaa gaatgagaaa
    20341 accttcctta gggcagttaa gcgctatatt ctgattttta gtcataagag caaatgaaag
    20401 ggtatttata ctgatgtcgt aatcaccttc acataccatg cgtataacgt ttgcaggtga
    20461 tccttcataa gtaattctgg tctgcagctc ttgaaagtct ttcgaagaga gactgttgag
    20521 atacgctcct gcgactctgc tcaaaggttt atccctggct gggaaaagta ctttgaactt
    20581 tttattgaac agatcggtcc atgagttggc cagatccgat tttttaatct tgtaaggatt
    20641 gtaaaacatg attagcggaa ctataaaagc aggataaaaa aaacagttgt gtgcagtaaa
    20701 gggtaaaaat tcttttctca gaggattttt ttgagtatat ctacaggcag atgaagaaag
    20761 aaaactttct ctttgactag cagaaaacga agcaaactct gtagcgtgtg taagcataag
    20821 atcaggcacc acatcatttt caaaagctct tccaagccat tgttcggtgc attgtgaaga
    20881 gtaaagttta tcgtgcggag taaaaacttc tatcttttct ccaatttttt gttcgaggaa
    20941 ctccaaatta gtatttacat aatccagcat ataaccacga agattaacgg gtgtatgtat
    21001 catgaaacgc attaagcatc tcctcctttt taaaaattat caacttcagc aggtaccact
    21061 acacggcgcc ggcatttctt aaaccggcat atatgtgtac atataccata aacgtccttt
    21121 atgttttttt cggtaattac atcctcactt gttccggcat tgtggatatg gccatctttt
    21181 aaaagatata caaagtcaga tacttctata gcatgattta tatcgtgaaa cactgctata
    21241 acagtttttt gaaaatgtgc tttcagattt ttcacaacac gcaggagatc ttgttggtat
    21301 cttagatcca tgtaagttac cggttcgtcc aaaagaataa attcagtatc ctgcgccaaa
    21361 accatggcaa tccatgctcg ctgtttttca ccacctgaaa gcatctccaa tggtctgttt
    21421 tttaaatgct ctacctgcat gtactgcata gcagagaaga ttttttgttt gtccagtgac
    21481 gtaaggatcc agccggattt atgataagga gagcgtccca ttgaaaccaa ttcttggacg
    21541 cttactccct gtagcttctg atgaaattgg ggaagaaatg cgatcttttg ggcaatttct
    21601 ttgggagaaa gtgattctat gggtttttcg taaagaaaaa cgctattttt ttcggtacgt
    21661 atcaatccac acaggcaacg taataatgta gacttgcctg aaccatttgg acctataagg
    21721 gaaattattt gattcttctc agcataaaag ttaatgctgt ttagaaccat atgatcagta
    21781 tacttaaagc taagatttct ggttattaat atgtaaatca ccaacctata tgtttacttg
    21841 cctacattat atcaaagaac ttgttttttg tcaatataaa caaaatttct aaaaggctta
    21901 taaaacttga caaaattaaa ataaatttat ataatataag tagataaaac tacataaaag
    21961 gaggtaatca tgagaagtct gcttctttat tcaacgctga caggaaatac tgaaaaggta
    22021 gctaaagcag tatatgaaac aatgccgcag ggaacagagt taaaaaaagc ttcaaattct
    22081 gaggagcctt cggattatga cgtcatagtc ataggttttt gggtggacag aggaacagct
    22141 gatgagaaga ttcttaagtt tgtagaaaaa acaagaggga aaaaagtagc cttctttttt
    22201 acgcttggtg cttatcctga ttcaccgcac gccgttaaga ctgccaagga ttttgaagag
    22261 cttctgaaga aaaacgataa tgaagtttta ggttacttta tgtgtcaggg caggatagat
    22321 ccgaaactta ccgagaagtt taaggatctg cccaaggatc atccccatgc tatgaccgaa
    22381 gaaagaagaa agcggcatga ggaggcaagc aagcatcctg atgaaaatga tttaacagct
    22441 gcaaaaaaag ttttttctga tattttttcc aagctaagat gaagtttcgg aataaaaaat
    22501 cccggcaatt attaaaattg ccgggatact tattagatac ttattcaact caaaccctgt
    22561 cagttttaag cagcagggtt ctgtcccttc cgttttcctt tgcttcatac agaacattat
    22621 ctgcatagct gagaaactct tgcgcactgt cgttaagtcc gggtattttt gaaaccagtc
    22681 cggcgcttac cgaaacacat actgtctgtc cgttaaggca aaaagcacgg tttttgatct
    22741 ctcttctgat attctcaaca tgattgaaaa agctctcgct tgaatcaccc atattcagta
    22801 cgataaactc ttctccgccg taacgggcta taaattcatt actttgggta aagttttttt
    22861 tcaaaatgta ggatatttct tttatacatt tatcgccgtt gggatgacca aacttatcgt
    22921 tgatctgctt gaaaaaatct atatcgaata tcactatatt aaagatgagc ctgttcctct
    22981 ggcaagtctg aaaaaaaggg tcaatattgt tgttaaagaa tcttctgttg tacagaccgg
    23041 taagatcgtc ggttacgctc agcatatgaa gtttattgtt aagcttttca agctcctgtg
    23101 ttttttgctg tatctcctgg gttctttcct gaacagcttt ttcgagatcg gaggtagatt
    23161 ttagaatttc ttcggtcata ctgttaaagg tgtccgcaag ttctttgatt tcgtcttttc
    23221 ctccgatact gatcttctca tactgattgc atcctatttt cctggaagcg tttatgagca
    23281 tataaatgga tttggtaatc cgtcttgaaa gcataaaggc taccgttgat gacacaattg
    23341 ctgaaataat aaaaagatag atgcttataa gtatcattct gtataatctc tggaaaagtt
    23401 cactgctcaa tacagtaacc gcaaaagtag actcttgcgt tttatgggag taagcctcga
    23461 tctttccgcc cccggtcttt aatgttttga acgtctggtc atcaaggttt ataagttttg
    23521 ataaaagctc actgtcgacg gaattttttg agtacaggag ctttccgtta ttgtcaaaga
    23581 taaagaaatt catggaattg aaggcacgaa gttcttgggg aagttcctgc catggaaaga
    23641 tattttcagc agttagcgag gtcatttcat ccgaaccgac cgaggttttt tctatagtcc
    23701 agtatctgaa aactgtaact gccgttttaa aaaattcatg gtttttttcg atactgtcag
    23761 tatagataac tataatataa aaagcaaata taatgaaagt aaaaatcatt attgtaagct
    23821 gtattcccaa aaaaatttta ttttttactt tcatctgctt caccttttaa agtagtttta
    23881 taaagtatat cattatatca tttgcatatt cttaaaaaaa tctttctttt tatctatatt
    23941 tttggtgtca gtctgatatt tctgttctgt tttttccaag acgtttggca aaataaagcg
    24001 caatgtcagt tcttttataa agcgaagaag cagtatcttc ctcttttgcc tgtgcaagac
    24061 cgatgctgat ggtgatcttt tccgactcaa aaaaatctgt tttctcaacc atggaacgga
    24121 ttttttccgc agcagtaaaa gcctgttctg aattgctttc tggaaaaatt accataaatt
    24181 catctccgcc gagccggaaa aaaacatcgc tgatgcgtat gttctgcctt acaatttttg
    24241 atatttgctt caaaagaatg tcacctttgt cgtgtccgta ggtatcgttt atacttttaa
    24301 agttgtcgat gtcaaacatt atggcacaaa aaggtctttt ataacgtctg agacgttcaa
    24361 gctctttcat cagaaaatca tcaaggcagc tgcggttata gatctgtgta aggctgtcct
    24421 tcatggcaag gatctccagt tcgttggcaa catacttacg gatatagagt attataataa
    24481 aaatacctat aagggaaatg atcatgaata tatatatggc tttaaactca gttactttct
    24541 tctctgaaat gatctgtgcc tcaagaacta tattgttagt tcttgaccat agtatctcac
    24601 ttattttgaa aagcgcatcc ctaacctcgg gttctctcga acgtctgaaa tcatagataa
    24661 gctctttggc tttttcccag tccagttcaa gctcatgaaa tacttcacca aattttatta
    24721 gtaaagaagc tttgggatgt tctgaatact ttttcataat actgtcgatg ctttcgataa
    24781 ggaagttgtc tgggttgttg ttcagctctg tttttataga tcgctgtata gaggctctta
    24841 ttatgccgct gaagtttata agattaatat catactctat gttttttaca aagataatta
    24901 cgacgaaagt gtttatagcg gcaacaagaa gtaagattag aaccataatg gtaagttttt
    24961 gtgatctttt catagtatca cctatattaa tattttttga ataaataatt gctaataaga
    25021 ttatatcaaa tagatgcaaa tgcacaaaat tataaaaagt tggttggaaa agattctatc
    25081 ataaaaattg gaagttgtca ttaaaaatta aaaaccgtct ctattaagag acggctagga
    25141 aggtaatttc taaaagatga tactgccgat ggtaaaaact atgaacgata ctacatatgc
    25201 tgctgcaaaa gaatatatta cactaaacaa agcccacttt gcgcctattt cctgttttat
    25261 ggttcctatt gttgcaacgc aaggggtata aagaagcgta aatacaagga atgcatacgc
    25321 gctaacacta ttgaaaaact gcggtaaaac ctgagaaagt atctcttccc cgccaaaaag
    25381 cgttcccatt gtcccggtaa caacttcttt ggcaagaaaa ccaaagaaaa gcgaaaccga
    25441 agcctgccag aaaccaaaac ccaaaggctt gaaaatagga gaaataatct ttccgaatat
    25501 gcctattatt gattcttcac ctgcgtactc cacaccggca ggaaggcttg caagcagcca
    25561 tattactatt actgctgcga atattacagt tcccgctttt ttaaggaaca taaatgctct
    25621 catgcctgct tctgaaagga tagattttat agaaggcagc ctgtatggag gcagttccat
    25681 tataaaaacg ctttcttcat ttttaaacag ggtttttttg aatatcagtg ctgagattat
    25741 agatatcaat attcccagaa cataaagggt gaatattaca aaacctgcat tttgggggaa
    25801 gaatattccg gccaccagag tgtaaaccgg tagtcttgcc gcacaagaca taaaagggtt
    25861 cacaagaatg gttacaattc tgtctctttt ggactctagg gttcttgtac tcattatagc
    25921 cggtattgaa catccaaatc caagaatcat tggaataaag gactttccat gtaatccaat
    25981 tttagtcatc agtttatcca taacaaaagc tgctctgggc atataaccga catctccaag
    26041 aaatgagatg aataagaaaa gaagaaatat gttgggcagg aataccacca cggaacctac
    26101 gccgccgatt atgccgttaa caataaatga agaaagcatt ccttctccaa gctttgctcc
    26161 agcccattct ccaagggcta caaaaccttc gtcaagcatg ccgttgaata tgtctcctat
    26221 agtaaaagta agagtgaatg tgagccacat tactaaaagg aatataggga ttccaagcca
    26281 tttgttggta acaaccttat caactttatc agaccaggat tcattgactt tctttcttgt
    26341 cacgtatttt tttgaaaggc tggagataaa cttatacttt tcttcagata ctattatact
    26401 tgggtcttct ttgaactcct ggctcattga agctgtaagc ttgtcaatat cctgcatggt
    26461 ctcctgactg accgagtctt ttatctcagt gtcgccttca agtatcttaa gggcagtcca
    26521 gccatagtcc acgaaattat cagaatgatg tttttttaag atctcctctg tctgcgacag
    26581 gtacttcata aacttttctg agtagctgaa aagctgcgtt tcctttccat tttcaagagt
    26641 ttggataata gcttgtttta gttcgttcaa gccttctttt ttgcttgcgg aggtttttac
    26701 tatttttact ccaagctctt tagccagatt ctctgtattg atgcttatac cgcggcgctg
    26761 tgtctcatca gacatgttga aagccagaac cacctttttt ttcatttcaa gaatctcaag
    26821 cacgagataa aggtttcgtt caagattggt ttgatctaca acaacaatta cggcatcggg
    26881 attttctttt attatgaagt ctcgtgcaat tctttcatct atggagtttg ccgtcaggga
    26941 gtaggtgcca ggaagatcca caatacttat ggttttttcg gctgccttaa actttccaac
    27001 ctttttttct acggtaactc cgggccagtt tccgacctgc tgatggcttc cggtaagtgc
    27061 gttaaagagt gtagtcttac cgctatttgg atttccgcat attgcaaccg tgttcatttt
    27121 ttttcatctc ccttttccac cgttatataa gaggcttctt ccttacgtat agtcagggaa
    27181 tagtttttaa tggttatttc tatgggatca ccgaaagggg ctttttttac tactgttatc
    27241 atacttcccg gagtaactcc catcacaagg agacgctttt tgacgtttcc ttcacccatt
    27301 atacttttga catatccgga aaaacccgga ttacattcac ttaatatcat ttgactgcct
    27361 cctgtaggct taccggactt accagtatct ttttggctgt acccattcca agaaagtact
    27421 ttatgccgtt tattctcagc attaccggcc cttgtgagaa atcactaagg acttcaaggg
    27481 tgctgccttc atttatgccc agctttgaaa gtcttgaccg aaggatcatt cctccgccta
    27541 tctttgaaac tttgaaaaaa cctcttcctg caataacaag tgacataatc ctcctcctgt
    27601 aatgttatac acttttagta gttataccta cattttatca caaaaaaaaa tcatagtcaa
    27661 atcacgttct taaagaaaaa agttcaaaag acagcaaacg tcagcgccta tcttttttta
    27721 cggtctgatg tggagataaa aaaagtaagc ctctgaaggc ttacttgatt accagtactt
    27781 cctttgcttc acttttcctt atggcaatgc tgtaatcgcg tatcttaatt tcgataggat
    27841 caccaagcgg tgcaacctta agaacttcga cttctatacc cgtagttatt cccattataa
    27901 taagacgttt atggatctgt tccgagccca gtatttctat aaccctaacc ttttcgcccg
    27961 gtttgacctg gtcaagacac atacgcgtct caagtgtttt ggatttataa ttttcaacat
    28021 acttgcggta tttggctttt atatcagggt tatcctcaaa aaaacttttt atcatttcca
    28081 tcgagcgcgt aagattatct gatttaagat gttccatttt gcaagaaaat tcatcggcct
    28141 caaaagcatt tatatcaagt atgttaataa agaagtctct tgtggtcatt ctgtttttat
    28201 atatcttctg ggctttttct atcccagact gggtaagctt tacaaagccc cttttctcat
    28261 agtttacaat tcccaaatct tcaagtttct ttatggcatc aatggcagag gtgtgtttta
    28321 cggaaagtga ggctgtcata tcctttattc gggctatctc atttctgaga tgagcctcat
    28381 aaattacaac cagataattt tccaaacttt cagataactc cataaaaaca catcctttgt
    28441 agtataatct acagcaattt taacattttt tttatattta gtcaaatttt aaagcatagg
    28501 aacattatag gttaaacata tcataaaata tatttttctc tgaaatacca gatgaaacaa
    28561 gcatctttac agcagcttca agaagacctc tgcttccgca aaggtaagcg cttaactctt
    28621 gaggaagttc tattttttcc tcaataaact tatgtgcaat ctctgtcaca agtcctcttt
    28681 cacatttaca tgaaacatcg tttttatcag caatacaagg tatgaaatga aagtttccaa
    28741 actgtttttc aaacttaccg aagtactctg tataaaagat atctttttgg gtctgtccgc
    28801 caaaaaagaa ccatatctgc ctttttttct cgttcttttg gagaagatct gtcactatgg
    28861 actttatcga ggacattcct gaaccgcctg ctatacatat catatcacgt ccgtcatcac
    28921 ggacatataa gtttccgaaa ggacccttga gttcgatttc atctccctgc tttagctttt
    28981 ggtgaatata tccggaaacc cttccgcccg gaacatgttt tactataaac tgcagagtac
    29041 ctgagtccgc aggtgttgaa gatatagagt aagttctgct tgttttggca ccttggctat
    29101 caggcataac agaaaatgac attatctgtc cggcaagaaa ggatatttcc tttggcgaaa
    29161 taagttcaag gtttatttct tttatatcgt gggtcagatt aataatgctt tttacaactg
    29221 tcttatatgt ctggggtttt gttctgctaa gtaccggctg ctccataagc ttcatctcct
    29281 tgtccgttaa agtaaaggca acgtactgtt attatatgtg attagaatgt cgggaaaatt
    29341 ataacaggtt taaatttttc taaccggctg tcggattaaa gtcttgagct tttcaggtgc
    29401 tgctcttctg ggatcgccca gatacacttc atggtgaagg cctgtcatct cgtatccttc
    29461 aagagatata aactcatgaa gcttttttac tgtaggttct tcctgtgcat atggtccaat
    29521 atgaagtatc tgagcacaca gcccttcctc gaagcattca aatgaaagca aagaagtatc
    29581 tatatcttct tttttctttt tcagcagttc cttaattcca gagtcaaaga tctcgctggt
    29641 tatgaactcc ggctgaacta taaaaacctt ccatttccat tcttctttct tttcaaggct
    29701 gaaggtacta atttcgtcgg tccaccagag tccttccagc ggcatgacag tataatgaga
    29761 agtatcggtt ttcttaagca taaatttgat ggtgtaggct atagaataaa gagtggacat
    29821 ggttttttgg aaaaaagcac tgttgttggg attgccataa ccgctgcagc ttattatttt
    29881 taacttaggt acggtaataa agtaaggttc agtttttttt gtatcgtaaa gatccttaag
    29941 tacttttttg agatcaaggt tcatagctca cctccggcag atgttattga tttgggtaca
    30001 gtagtattat aaaacaaaaa gtatgacaca gatggtcata cttttattca ggtcattcct
    30061 aacggaggat aaatctttta ggaataagtt ctttcatgga agatactgta tatatgtcgg
    30121 tgctttgggc tacggttata taagctcttt ctttttcctc aagcagtttt ttgaagaaat
    30181 cgatatcgta ctttgaagag agcccaggta catatgtcat tctcagttcg tactctagat
    30241 ctgatttcct taaaatatca acagtttgta agacctttga aaaatcagta ggagtacctg
    30301 taagcttttg atagttttca ggagcagcct ttatttcaag agcgcagtaa tcaataagct
    30361 ttttctgtgt aagctcgcta agcatctgag gatttgttcc gtttgtgtca agttttacat
    30421 agtatttgag agtatttttg agatactcta taaccttagt aagatcttta tgtatggttg
    30481 gttctccccc tgttatacat atggcgtcta ctcttttgcg aagaagttca gtctgcttaa
    30541 agatttgttc aatactcatc tgttcttttg cgtctgagta agctatgctg tgattgtggc
    30601 accatggaca tctcatattg cagccttgcg taaaaagcac tgcgcttaca tagtccggga
    30661 catcgacgaa agaaaaggac cttattcctg caaacatttg gaatcacttc tcaaggagaa
    30721 ctggactctg tgaagatatt cctgtttttt accggcgttc cagaccttta cgggtctgta
    30781 atatcccaca attcttgaat aaacttcggt ttctcttgaa cagatgggac agacgaactt
    30841 ctcaccggat atatatccat gcacaggaca tatggaaaaa gtaggggtga tactcatata
    30901 cggaagtctg taattcgtaa aggctttttt aataaagtcc tttaagacat ttgctgaaga
    30961 aatcttctct ccgacaaaga tatgtaccac tgttcctccg gtccacttgg tttgtagctc
    31021 tttctgatgt tcgagcagat caaaagggtc atcgaaagag ttaacaggag gatgaacaga
    31081 gttagtatag tatggagttt cttctgttcc gctgaaaaaa gcggagtttc catactcttt
    31141 ccgatcaata caggcaagac ggtatgaggt tccctcggca ggtgaggctt ccaggttata
    31201 aagattatta gtttcattgg tgaactcttt aagagtatca agcataaagt ccattatttc
    31261 aagggctttt tctgttcctt gccggttaag tatcccatcc ttgattccca tattaagcag
    31321 cccctcatgc atgccgacca gaccgatggt tgaaaaatgg ttcgcccagt actgtccgct
    31381 gagtgcatag atcttatcaa gatataccct gcagtagggg aacattccag atacggtaag
    31441 ttcttctatg tatctgcgtt ttatttcaag actttctttt gcagtgttca tagcatcctt
    31501 aagaagtgtg taaaattttt tgaaatcatt atgactcatc atagcaattc tgggaatatt
    31561 tattgttact acacctatgg atccggtaag agggtttgag ctaaaaagac cgcctctgcg
    31621 tatggaagta tcgttttcat aacttttatt tgagatattg aaggactgag aaagctttcc
    31681 gatatgattc atcacaacgg tattatcaag cctgagtcta cagcacatgc tccttacatc
    31741 ttggggtgag atatccgaac ttatgtaatt tgcaaagtaa ggagtgccat acttt
//
LOCUS       DAOSHR010000003        27697 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_1348_length_27697_cov_5.862383, whole genome shotgun sequence.
ACCESSION   DAOSHR010000003 DAOSHR010000000
VERSION     DAOSHR010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 27697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27697
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1348_length_27697_cov_5.862383"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(180..1082)
                     /gene="ftcD"
                     /locus_tag="PLU28_00275"
     CDS             complement(180..1082)
                     /gene="ftcD"
                     /locus_tag="PLU28_00275"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HOG33528.1"
                     /translation="MSFGKIIQCVPNYSEGKDLEKVEMILDCFRGKEGVKLLDYSSNK
                     DHNRTVVTLIGEPEPLMQAVIKSVGVAAELIDLSKHKGEHPRMGATDVIPFIPIRNTD
                     MKECVDLSVKVAGAISEKYGIPTFLYEKSASSPQRANLADIRKGEYEGMFEKIKRPEW
                     KPDFGPAVNHPKAGATVVGARMPLVAFNINLNTNNLDIATKIAKNIRYINGGLRYVKA
                     LGIDLKERNIVQISINMTDYTKTSLYRAYELVKIEAQRYGVVPVGSEIVGLVPMQALI
                     DTAEYYLGLEDFSNNQILETRIWE"
     gene            complement(1200..1838)
                     /locus_tag="PLU28_00280"
     CDS             complement(1200..1838)
                     /locus_tag="PLU28_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOG33529.1"
                     /translation="MIEELLKATIETLYMTFFSTLIAFMAGLPLGILLYLLSKKKDHA
                     SKIIYTFFDFIINIFRSIPFIILIILLIPLTKLIAGTIIGANSAIVSLSIAAIPFMAR
                     LCENSFNSLSHEMLDTASSMGMTTWQLITKVLIPESLPEIISNTTILIINLITYTAIA
                     GAVGAGGLGAMAINYGYQRFRADILLYTVSILVIITQLVQFAGTLLSKRLRR"
     gene            complement(1835..2836)
                     /locus_tag="PLU28_00285"
     CDS             complement(1835..2836)
                     /locus_tag="PLU28_00285"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HOG33530.1"
                     /translation="MLRLEDVNLFYQKNNHVLKNISFETQKEEILGIIGLSGAGKSSL
                     LKTFNLLQRPSSGKIFLDNILITDLKKKELRQVRKQISVVFQNYNLLNSRTVFENVAL
                     ALELEHQKFIEPTVLNVLEKVGLSHRKDAYISQLSGGEKQRTAIARAVVTNPKILLLD
                     EPTSALDPKTTEKILDLVLYVNKELGITTIVVTHEMEVIKRICDRVLYLKDGTIKFLG
                     QTHRLFIEKDSQILEDFYEEINIDKELINQKLEKKESHLLRIVFWGSSTHCPVISTLS
                     LKYSVTSNILYGKIEHLKNNPYGTLILEISENPNYSDFLNELKKNVYFCEELTGGGK"
     gene            complement(2845..3663)
                     /locus_tag="PLU28_00290"
     CDS             complement(2845..3663)
                     /locus_tag="PLU28_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HOG33531.1"
                     /translation="MLKKRSLFVALLVALIVLAVVLVITLNPSKEKVFKIGASPVPHN
                     EILEQVKDDFRKETGYELKIIIFTDYVQPNIALEQEDIDANFFQHIPYLESFNLKNGY
                     TDLISAAKIHVEPMGLYLKKPLNELSPSDTVLLPNDSTNEGRALMLLENNGIITLRSE
                     NKLIATVKDISENKYGINFLELEAPLLPRTFKEDSKIAGAVINTNYAIEAGLNPISDS
                     TFIEGSDSAYANIIAVKEKYIDSKLLKSLISLLQSEKIKSFINSKYNGAVVPVF"
     gene            4001..5230
                     /locus_tag="PLU28_00295"
     CDS             4001..5230
                     /locus_tag="PLU28_00295"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOG33532.1"
                     /translation="MFEFFNDWLETNGVKGLFSDVLTTLFIIGIIALVSYLSYKISKV
                     ILQRTMKHFVEKSKTRWDDVLLKRGFFVRLSMFFPAFVIYGFSSAFADYQDIIQRCVF
                     SYMIFVSLLTLNAFLNAFTDMYSYHEVSRQRPIKGYVQVFKIFFAIIAVISIVGTLIN
                     KPFGYLIGGIGAMTAVFSLVFKDTILGFVASIQLSANDMVRIGDWIEMPKYDADGEIV
                     DISLNTVKVQNWDKTITTIPPYALISDSFKNWRGMQASGGRRIKRSIFIDMNSIKFCD
                     EQMIKKFSEIRYISEYITSKVKEIEEYNRLMGADDSNPVNSRRLTNVGTFRIYILEYL
                     KRNPRLRKDMTLMVRQLANNERGLPLEIYAFTNTIIWVEYEAIQSDIFDHILAVLPIF
                     ELKLFQNPTGNEFKILH"
     gene            complement(5267..5608)
                     /gene="aroH"
                     /locus_tag="PLU28_00300"
     CDS             complement(5267..5608)
                     /gene="aroH"
                     /locus_tag="PLU28_00300"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HOG33533.1"
                     /translation="MIVDIRGATSVENFSIQEISERTKELMEELFRRNKVERIISITF
                     SVTEDINLINPASIARKAFGLDHVSLMCLKEAQFEGSINGIIRVSLLCESPDSNFVYL
                     HRACMLRPDLK"
     gene            complement(5598..5927)
                     /locus_tag="PLU28_00305"
     CDS             complement(5598..5927)
                     /locus_tag="PLU28_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG33534.1"
                     /translation="MEKSVRTTPTFKLDAYAKKDVLTKLKQLCTELKLEENITDISIR
                     AGRVYLTFSQEINARISIYDGSLDHCSLERPYSNAKWKSVSEGSMKACLTFIKEKILL
                     SGGKGDC"
     gene            6126..8291
                     /locus_tag="PLU28_00310"
     CDS             6126..8291
                     /locus_tag="PLU28_00310"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOG33535.1"
                     /translation="MKILFFLITLWLVSVPLYADKVDSYGKDLHSIKLQLKWRHQFQF
                     AGYYAALEKGFYEQAGLDVSIIEGGPDIFPVAEVYNKEAQYGVCGSELLLHRLRGYPL
                     VLIANIFQHSPLVLMVVDKSGIYSPIDLRGKKILLHEDGSDAEISAMLMSEGVDPQSY
                     GYYDGSVDTDMLLEGKIDALAVYITNEPYLLKSMGMDIRVINPLKYGIDFYGDCLFTT
                     EEEINNYPLRVRAFRQASIKGWEYALNNIDEIALLIKEKYRSDADIDMLRYEGSTIRD
                     SFIMNKEVELGHTNQGRWEEIGKTYRRLGMTASSSRMEGFFAWEYENNESPEKLPSKL
                     LPYMGIGILSLLAGGTLFFHIKLKVYTKNKIHSLQNFFSLPSYMLLVMDYSGKIKNYN
                     QSWALLMKRYFGSEHPENIRQVLTGEDVGKIEKFCHMNDPLQSLISLEVFFKNAESSV
                     WINLDFHQAFDEKLIYGIARDITEEKARNERMGESVEIAMENARKKTDFIASMSHEIR
                     TPMNAMVSITQMLIESGLSIEQKDIAEILMVSQHNLLQIINEIIDFSKLEAGKDEVIL
                     DSFSIKEQAKSIYQLFEVMARQKKLDFKLNLIRPLPNFIISDSQKIRQICINLISNAF
                     KYTEKGEIIFSVGLTEAGILKISVQDTGIGISKDDIGRIFNFYSRIERKGTVRQSGTG
                     LGLVISQKYARAMGGAVTVESQVSKGSVFTLTVPVHVIN"
     gene            complement(8326..10050)
                     /locus_tag="PLU28_00315"
     CDS             complement(8326..10050)
                     /locus_tag="PLU28_00315"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG33536.1"
                     /translation="MIKKFISYYKPHRGLFYLDIICAFLISSSDLAYPMVTRYIINTV
                     IPDKNLSKIFAFGLFLLLLYFARMVLEYIVGYYGHILGLRIQYDMRKEIFSHIQKLPI
                     SYFDNTKTGHLMSRVTNDLFEISETAHHAPEDLFLSIVKIIGAFILLCTINWQLTLIV
                     FLIIPFMFYFMVVYNTKLENTFRRAKESYANINARIEESISGIRVVKSFTNERFENTR
                     FDEGNEKMKNIREETVKQIGIFDSTIHFLSNSSTVIAMVAGGYFVYTGSINIGDFVAY
                     IIYMTQFLQPLNILLRFVEQYQEGMAGFRRFVEILKLQPETELSNEKFLENVHGEISF
                     KDVSFSYDDKNTVLKDINLTIKKGESVAIVGPSGSGKTTLCSLIPRFYDIDSGSITID
                     SVDINTVCLESLRKNIGIVQQDVFLFSGSIRENVAYGKLDATDDEIKLACIKANADEF
                     ITAMPYGYETIIGERGIKLSGGQKQRLSIARMFLKNPPILILDEATSSLDNKSESIIQ
                     NSIEELSKDRTTLIIAHRLATIRNSKRIIVLTENGIEEEGTHEQLIRRKGVYYNLYNA
                     QFESLLIG"
     gene            complement(10063..10971)
                     /locus_tag="PLU28_00320"
     CDS             complement(10063..10971)
                     /locus_tag="PLU28_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG33537.1"
                     /translation="MTITVRSEPIEFSGMVAVCKNCGNEIDNNVIYLRNLFAAYRIYE
                     KKHFLAGSQQLKEYEKLFMCTLEELSILMEIPHSTLKKYTAGDLMKKEDSEKIKTFLE
                     PKNFFELLEKNKKLMTSESYKKILEKLKTFNGIVKEKNPYEAIVLKAHPVIDIERVKL
                     SFMAVTSIIPEKSYIRLIRFMWLCDKFFYNSSARPFTGISYIKHQSGIDIEKRTEFLS
                     YFEFEGLIELSGTASSEDILRISITPKKSFQTAIPDENFKVLQSVCNSYKSLGNDALT
                     SFIYKFEEFRNIKNNQQIIFSKNGNV"
     gene            complement(11038..12408)
                     /locus_tag="PLU28_00325"
     CDS             complement(11038..12408)
                     /locus_tag="PLU28_00325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOG33538.1"
                     /translation="MDHSKVLGEESIEKLIVKFSIPAIIGMLVNALYNVVDRIFIGNG
                     VGSLALSGLTVTFPIMLISMALSMLIGLGATSLISIKLGEKKKEEAEKIAANAFTLLI
                     GVSLIYSVVFLLFLDKILLLFGASQNTLAYARDYLSIILYGSIFQFIGFGMNHFIRAE
                     GNPKRAMSTMVIGAVLNTVLDPLFIFVFKMGVQGAAIATIISQAVSATWVLYYFMAKK
                     STVKIHAANMKLSWKYVRQIVSMGLAPFSIQLVGSVYNLILNNSLARYGGDKAIAAMG
                     IISSAAMIVMMPVFGINQGTQPIVGYNYGAEKYDRVKKALMYAYIGATFFVVLGFLSA
                     MFFPTQIIQFFNRTDGELVSIGVPAMRIFSLLMPVIGFQIVSSNYFQAIGKPKKSMFL
                     TLLRQLIVLIPMVIILPLFFGLNGIWYAVPMSDGISTMLTTVFMYFELKELNKKITET
                     VKVPAI"
     gene            complement(12530..12772)
                     /gene="hfq"
                     /locus_tag="PLU28_00330"
     CDS             complement(12530..12772)
                     /gene="hfq"
                     /locus_tag="PLU28_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HOG33539.1"
                     /translation="MAEKTKIQDKLLNIARVNNIELKIFFEGTFSISGYIKSFDEFTV
                     LIEKDGQQTLIYKSSISRIDPMKTIMLFPKDSTFKK"
     gene            complement(12785..14134)
                     /locus_tag="PLU28_00335"
     CDS             complement(12785..14134)
                     /locus_tag="PLU28_00335"
                     /inference="COORDINATES: protein motif:HMM:NF014045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HOG33540.1"
                     /translation="MKTSFILMDYQNQPVDPELIVKKIAEFAKVIGGKAYGHWSKYPA
                     TMFTFLKYGIELIEMPEDGFSNKKGNDIKLAVDTIESMFMFPHIESYVLVTGDADFLP
                     LVRKLKTYGKSVIVISRSKTTSADMQLAADMYIPYEDIVKNESIVDIDTFDDLVEEII
                     RKTDEKNLSPDEAIVKRIITGIGVDYSKFRYTSMNAFVEDILKAIKCRLMNSDEVSEY
                     EENYMRFMERLIVTSITPVDYKSLESTAISRHPWISSNSRTSLKDLIKNMIESKRLIK
                     TDSGILKVPAPRRWEIKYEKLLPYPEYRNNFVNMTFKSFAEKKSNTIYEVLHQAKDSL
                     GLTNKAISSFGIALKFSGMFLGKDGSDYVSIKTPVIIKGSFEQLKASMDTFYIKRILK
                     DEDIHSQDIKYVSEYVFDNQDEKTCISLLKELIQKNEVVFEKPFYKYIRRTQNNEEN"
     gene            complement(14178..16931)
                     /gene="ileS"
                     /locus_tag="PLU28_00340"
     CDS             complement(14178..16931)
                     /gene="ileS"
                     /locus_tag="PLU28_00340"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOG33541.1"
                     /translation="MDYKDTLNLPSTTFSMKANLKEKEPEILKKWEGLDIYKYVQQTR
                     KDSEMFLLHDGPPYANGHIHMGHALNKVLKDIVIKYKTLRGYNSPYVPGWDTHGLPIE
                     HKVTTELGEKAKQLSKNQIRDLCREYALKHVQIQKEEFKRLGVRGNWDEAYVTLDPVY
                     ESQVLDVFNSFVKQGNVYRKKKPIYWCAECQTALAEAEVEYHDHSSSSIYVKFEFDVN
                     TYIVIWTTTPWTLPANVAIAVHPEYDYCKVKVGNEYWILAKQLVPKVMKDAKIDSYEI
                     TEVFKGSSLENKSAKHPFIERQSLIVLADYVTLEDGSGCVHTAPGHGADDYTTGLKYN
                     LPVLSPVNHEGIFTKEAGKYEGLKIWKANEVIINDLKASNHLIASQKITHSYPHCWRC
                     KSPIIFRATEQWFISVENNSLRQKVLNEIKNVQWIPEWGETRITSMIHDRPDWCISRQ
                     RAWGIPIPALYCENCTEVLLDPQVLEKFISIVKAKGTNAWYDMDLKELLPDGFKCPKC
                     GSDHLRKQYDILDVWIDSGASFQAVIHAREELKKFPADLYLEGSDQHRGWFQSSIFMS
                     VGKHGKAPYRKVLTHGFIKDEEGKKMSKSIGNVIAPQEIIDRYGADVLRLWVASSDYR
                     GDISISYKILDQQVDVYRKFRNTVRFLLGNISDFDPKKDLVKYEEMPELDRWAMMKLH
                     ELIRDVTQAYESYEFYRVHQMLNNFCTVSMSSIYLDIIKDRLYTLSKKSQMRRSAQSV
                     MYEILMAMTKMMAPIITFTTEEIYDHLSENFEKKATVQAESWPEFNENYIDKALEEKW
                     NRVLLIREDILKALEEKRRDKFIGHSLESKVTVQPKEPSDCAILDSLGNEFMSDVIIA
                     SGFETGTVENGYEGSLCRVRVEKASGKKCERCWKIDEHTGEDPQFPDTCPRCAHTLKT
                     MNS"
     gene            complement(16953..17309)
                     /locus_tag="PLU28_00345"
     CDS             complement(16953..17309)
                     /locus_tag="PLU28_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG33542.1"
                     /translation="MARILIVDDEDNIRLLIKEELLDGGYEIVDANSAKKALQILSAD
                     RNFDLVCTDIEMPDINGLELAGDIRKKYPEMKIIFLTAYSHYKSEMASWAADAYVVKS
                     MDLTELKDTIQNLLKL"
     gene            complement(17342..20095)
                     /locus_tag="PLU28_00350"
     CDS             complement(17342..20095)
                     /locus_tag="PLU28_00350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG33543.1"
                     /translation="MIDSILKIIEHQYPKDFFTSITKEIALSLREKFGIDSFIFIFEN
                     ERNILKLLGSSMDMDPRDDDLKVYYNDFPSLFEELKKNNIVELGFNEFFSIYTLKEEK
                     TVFFYPIFSGESFMGAVVLSKQYIQAENLREALRSYLVVLKLVVDTSSVNAFREKLGI
                     FEKLSDIFDNSQSDETLINNILCLLKDTLFAEVAAYWKYEDEKLVLYNISGLEPSEVK
                     KNILPIGSSVEGSLLNGEESLLLIGNTNFEKYENPFDIHMKSAVYSRLSVDNVTFGIV
                     AIYNKKEREHFLPYKYFDSIDQIFLNSTVKRMGLGLSRINIYNRLKNEIQNLKELKQR
                     HEESIRVQKEQLDKMNSLHKISQAIRSTYDKNNSIKIMLLGLTSGRGLRFNRALYLEC
                     DRVRGFLIPKLWIGPDEDEDVDKIWKEANRRALKYGDMIQYLREEALSLPFTNKLTEN
                     VKNMVLVYKGHPILDRVVSKKQVIHVVPQMLKIKHDELEDIYELIRTQEFLIFPVTGR
                     FDTRGIVIIDNKFTGTPITNVDIEIIKLYQDSIGLGLEMIENYDELKEKTKSLEEQKN
                     LIDYYRRFKDNILQNLSVGIVVVDRTGKILEWNQKAEVFFSKARESIIGTSIKLLSGT
                     FNDDILNTIEQIYDTKTDLRFPNYKLNVHEEEKIFDIEFSPLWNRDLGVIEGVIIVFG
                     DITEVYKLQKEIEKREKLSAIGEMTARIAHEIRNPLTVIGGFLNRMLKKLDDPQSIRK
                     YSGIIQSELTRLEDIVSEILEYSRGERLPTFEKVSIGTILDDVVMMFEDFISQKEINI
                     QVHKNEPELFTNGDKSRLKQVLINLVKNAIEVVPQKGSIKVHSFADDSTIVIEVENNG
                     TAITPEIKEKLFLPFFTTKTNGTGLGLAICKKIIEEEHKGKLFLLKSDDFSTVFRIEL
                     HSC"
     gene            20236..20922
                     /locus_tag="PLU28_00355"
     CDS             20236..20922
                     /locus_tag="PLU28_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HOG33544.1"
                     /translation="MFNISVIVNAVAIIAGGILGLLIGKKLSEKIQKILFTSVALTTI
                     IMGVQMGLKAENFLVVLGALSIGGVAGEVIDIEGRLARLAGRIEKSDGQTKFVKGFVS
                     STVLFVVGPMTVLGCINAGITNDNSIIYLKSLLDGISSVIFGSVYGFGVIASAASVMI
                     IQGLLVSFAGSLSFLSDEAYLLNFTAVGGVMVLAIGIRLLDIKEIKVGNFLPSLGAVI
                     LLDYIKTLIA"
     gene            complement(20937..21209)
                     /locus_tag="PLU28_00360"
     CDS             complement(20937..21209)
                     /locus_tag="PLU28_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG33545.1"
                     /translation="MIPVAWWSPAKKDSNRDIAVKRMNDLVTNRREACATAIKFENKS
                     EEEIESIVTYIKDYACRNLSVNEKDVKVHISKDGNIVTIIASIAYK"
     gene            complement(21206..22021)
                     /gene="minD"
                     /locus_tag="PLU28_00365"
     CDS             complement(21206..22021)
                     /gene="minD"
                     /locus_tag="PLU28_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HOG33546.1"
                     /translation="MDDSKVFVVTSGKGGVGKTTVTANVGACLALLGYNVCLIDADIG
                     LKNLDLVLGLENRVVYTIIDVVRGNKDPLEAVVKHKLLKNLNLLASSQIANKDMVSEQ
                     DMINLVNELSKHYHYIIIDCPAGIERGFKNSIAPAQQAIIVTTPELTAISDADRVIGM
                     LENQGFSEQRVSLIVNRLKPRMVQRDKMVSPEEIKNALAVELLGVVPDSEEIIVSTNE
                     GNPLVLDERNRLSIPFKNIAQRILGNPIPISEDIAGSSEDGFLGFIKKLLGRR"
     gene            complement(22193..22375)
                     /locus_tag="PLU28_00370"
     CDS             complement(22193..22375)
                     /locus_tag="PLU28_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HOG33547.1"
                     /translation="MKVFVDKDACIGCGVCENLCPDVFKVGDDGKAEAIAPQTDAPCA
                     KDAADSCPVQAISIEE"
     gene            complement(22453..23382)
                     /locus_tag="PLU28_00375"
     CDS             complement(22453..23382)
                     /locus_tag="PLU28_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HOG33548.1"
                     /translation="MLIHINDPLDKQATQTLSKLLPGHTIISQHLETADLIAKMPEID
                     VLVIRSATKATKEIIDAGTHLKLIARAGMGLDNVDQNAAKARNIKVINTPGANSLSVA
                     ELVAGMILSIDRFLVRSTTGMIEGKWEKKSLEGFELSGKTLGIVGFGNIGKLLSKILS
                     GFNLKVMVYDVFEIAQETLKEYNAKQVSLEEIYSNCDIISFHVPKTEKTTHMVSDKEF
                     EMMKKGVIIVNASRGGIIDEPALLRALESGKVFGAGLDVYEEEPPKSEVYQKLFKMPN
                     VILTPHIGAATVEAQWRVGMDIVDRIVKEVKNF"
     gene            complement(23384..24523)
                     /locus_tag="PLU28_00380"
     CDS             complement(23384..24523)
                     /locus_tag="PLU28_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HOG33549.1"
                     /translation="MVRKNYLMTPGPAPVPIDILLAGAQDTIHHRTPQYLEIQKKALE
                     GAKVLFQTSNDVMLMSSSGTGAMETAVSNLVNPGDKVIVINSGKFGERWLKLCKTFGA
                     NIVEEKLDWGVAIQPERLSALLKEHPDTKIVFTQLNETSTGGRQPVKELAAITRNTDT
                     LIVVDAISGLLAMELKMDEWGVDVVITGVQKGFMMPPGVALISLNERAWKAVENCKTP
                     RFYFDLRQYKKNYPDSPWTPPINLIYQLERAVEMLLKEGIENIWARHELLAQATRAAV
                     QALGLKLYAKDPGNVLTSVCMPENISSSKIIKMLRDDWGVVFADGQDDVKGKIARIAH
                     LGYMSQFDIITAIAALEMGLAKFGYNVELGKGVRAAQEVFMKAGI"
     gene            complement(24812..24887)
                     /locus_tag="PLU28_00385"
     tRNA            complement(24812..24887)
                     /locus_tag="PLU28_00385"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24852..24854),aa:Trp,seq:cca)
     gene            complement(24938..25294)
                     /locus_tag="PLU28_00390"
     CDS             complement(24938..25294)
                     /locus_tag="PLU28_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG33550.1"
                     /translation="MKKNLVLIFIMTVSITLAFFKFTYDIFISIGFFTVFLTSVYGFF
                     AYDPIWYHKSAHIIISSMIGFLIGTYELFKYLSSFLSTVVDPSVLPSADLWTVFFGIL
                     CFLVMIYEVAMLKKSK"
     gene            complement(25291..27240)
                     /gene="feoB"
                     /locus_tag="PLU28_00395"
     CDS             complement(25291..27240)
                     /gene="feoB"
                     /locus_tag="PLU28_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HOG33551.1"
                     /translation="MAFEVSIIGNPNVGKTSLFNIITGTRQYVANWPGVTVEKKIGSF
                     SYKGHNFKLVDLPGIYTLSACSEDERVARDYFLNESPDAVIIVADALNLERSMHLMIQ
                     ILEMNLPAVLAINSIDEAEEKGRIINPEVISKTFNIPVVLTSAKTMKGIPQLLEAIHR
                     TSEQKKFHDINFQYPVEVNEYIETFKSKAAKYPSLSGFDHEWLGIYFLEFGAGSIHTE
                     AELSQSICTSFDMKKLKDIYLNWKYGLIRNLTDMSIVSLGRSWSLRDVLDHVLTHKVL
                     GLLIYLVALFFVFSMTFSIATPLSDLLNLFFSKLSSWAGSVISVPWLKSLVTTGIIEG
                     VGGVLAFIPQIFVLFFFMGFLEESGYLPRAAFLVDKIVRKFGLSGRSFMSIILGFGCN
                     VPAIMSTRTISSTKERLALLLSIPFASCSARLPVYIIIIGAFFPKHAALIMFLLYISS
                     ILLVLLSAKLLQVFVTKTDDIPFTIELPRFRLPTAKNLFLYTWEKTEHFLRKASGIIL
                     IASILIWFLSYFPSGSDIQNSFAATIGRSFEFLTRHLGWDWHTNTALVFGVAAKEVIV
                     STYAATLNTDPAALKDALASSMSPQSALALLYFIMAYIPCFATLSAVKLETASYKWAA
                     FTFIYTIFTAYILANIVFFIGGLIL"
     gene            complement(27253..>27697)
                     /locus_tag="PLU28_00400"
     CDS             complement(27253..>27697)
                     /locus_tag="PLU28_00400"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HOG33552.1"
                     /translation="PLSSLKVGMKGKIQSIDFEEQIKERFLAMGFVPGKDIELVHVSP
                     FGDPMVFLIDDNKIMIRREQAGKILIDVGFLIETLFSIPDGMCEVFTIDSGMMLYERL
                     KSLGIYPGITGEKHLYKGKIFFRTNQKDILLGKRISRKILCKKYV"
BASE COUNT         8069 a         5387 c         5256 g         8985 t
ORIGIN      
        1 ctgatctgtt tcaactttcc ctataaaaga tattttgccg ttttttataa gaatatctgc
       61 tccttcgatc ctgcctatat ctctcatctg tacgcctttt ttggctgaaa agcctgaaca
      121 ggtaagtatc tcagaagcgt tttttatgag gaggctttca cggaaattga tagtaccacc
      181 tactcccaga ttcgtgtttc gaggatctga ttatttgaaa aatcctcaag tcccagatag
      241 tactccgctg tatcgatgag agcctgcata ggcacgagtc ctacgatctc acttcctaca
      301 ggaaccacac cgtaacgttg agcttctatt tttacaagtt catatgccct gtaaagggat
      361 gttttggtat agtctgtcat atttatggag atctgcacaa tatttctttc cttaagatct
      421 attccgagag ctttcacata acgcagaccg ccgtttatgt accttatatt tttggctatc
      481 tttgttgcta tatcaagatt gttggtatta agattaatat taaaagccac aagcggcatt
      541 cttgcaccaa ctacggttgc tcctgccttg gggtggttta ctgccggacc aaagtccggt
      601 ttccattccg gtcgcttgat tttttcaaac atgccttcgt actcgccttt tcttatatca
      661 gcaagattag ctctctgtgg tgaagaagcc gatttttcgt aaagaaaggt cggtatgccg
      721 tatttttcag atatggcacc tgcaactttc actgaaagat ctacacactc tttcatatct
      781 gtatttctta taggtataaa aggaatgacg tctgttgctc ccatcctagg atgttctccc
      841 ttatgttttg aaaggtctat aagttctgcc gccacaccta ccgatttaat aactgcctgc
      901 attaaaggtt ccggctctcc tataagggtc acgacggttc tgttgtgatc tttattgctg
      961 ctgtagtcaa gaagcttaac tccttctttt cctctgaaac agtcaaggat catctctact
     1021 ttttcaaggt cttttccttc gctgtagttg ggaacacact gaatgatctt gccaaaagac
     1081 atctcaaata cctcctgttt gcatgctgaa ataaatttca taccatactt attatatcag
     1141 gaagaaagct aaaaaaaaag aatagcttgt aaccgaaaaa ttcagaatag gccggcaaat
     1201 tacctgcgca gcctttttga cagcaatgtt cctgcaaact gaaccagttg tgtgataata
     1261 acaagtattg agaccgtata cagaagtata tccgctctga agcgctgata accgtagttt
     1321 atggccattg caccaagtcc tccggctccg acagcaccgg ctatggctgt gtaggttata
     1381 aggttgatta taaggatagt ggtatttgaa ataatctcag gcaaactctc tggaatgagc
     1441 actttggtta ttagctgcca tgtggtcata cccattgaac ttgccgtatc aagcatttcg
     1501 tgagaaagac tgttgaatga gttctcacag agccttgcca taaaaggaat agctgcaatg
     1561 gaaagagaaa ctatggcaga gttggcgcca ataatagttc ctgcaataag cttggtcagt
     1621 ggaataagga gtattataag gattataaaa ggtatggaac ggaaaatatt gataataaaa
     1681 tcaaagaaag tataaataat cttactggca tgatctttct tttttgaaag cagatacagc
     1741 aaaattccca gcggaagtcc ggccataaaa gcaataaggg tggagaaaaa ggtcatataa
     1801 agtgtttcta tggttgcttt taagagttct tcaatcattt tccgcctccg gtaagctctt
     1861 cgcagaagta tacatttttt ttcagctcat ttaaaaaatc agagtagtta ggattttcag
     1921 agatttcaag tataagggtg ccataggggt tgtttttgag atgttctatc tttccataaa
     1981 ggatattaga agtgacagag tactttaaag ataacgtgct tattaccgga cagtgggtag
     2041 aacttcccca gaacacaata cgtagcagat gactctcttt tttttcaagc ttttgattta
     2101 taagctcttt atcaatattt atctcttcat aaaaatcttc aagaatctga ctgtcttttt
     2161 ctataaaaag tctgtgagtc tgtccaagaa actttatggt tccgtctttc aggtaaagaa
     2221 ccctatcaca gatcctttta ataacttcca tctcatgagt aaccacaatg gttgttattc
     2281 ccaactcctt atttacataa agaacaagat caagtatttt ttcggtagtt ttaggatcaa
     2341 gagcagatgt gggttcatcc aaaagaagga ttttcggatt agttaccaca gctcttgcaa
     2401 tagcggtacg ctgtttttct ccgcctgaga gctgcgatat gtaagcatct tttctgtgcg
     2461 aaagtcctac tttttccaaa acattcagta ccgtaggctc aataaacttt tgatgctcaa
     2521 gttcaagagc cagggctaca ttttcaaata ctgttctgga attaagaagg ttgtagttct
     2581 gaaaaacaac gctgatctgt tttcgtacct gcctgagctc tttttttttg agatctgtaa
     2641 tcagaatgtt gtcaagaaaa atttttccgc tggagggtct ctgtaaaaga ttaaatgttt
     2701 tgagcagaga ggactttcca gctccggaaa gtcctattat tcctagtatc tcttcttttt
     2761 gtgtttcaaa gctgatattt ttcagcacat gattgttttt ctgatagaat aagtttacat
     2821 cttcaagtct taacatgaac tctcctaaaa gaccggaact acggctccgt tgtattttga
     2881 attaataaag cttttaattt tttcagactg taaaagagag atgagtgatt ttaaaagctt
     2941 tgaatcaata tatttctctt taaccgcaat tatgttggca tatgcagaat cgcttccctc
     3001 aatgaaggtg ctgtccgaaa tcggatttag tccggcttca atggcataat ttgtgtttat
     3061 gactgctccg gcaatcttgc tatcttcttt aaaggttctg ggaagcaaag gagcctctag
     3121 ttccaggaag tttattccgt atttattctc tgagatgtct tttacagttg caataagttt
     3181 gttttcggat ctaagggtta taattccgtt attttcaagg agcatcaagg ctcttccctc
     3241 gtttgtagaa tcatttggga gaaggacggt atctgatggt gaaagctcgt taagtggttt
     3301 tttaagataa agtcccatag gttcaacatg aattttggca gcacttatga gatccgtgta
     3361 accgtttttg agattgaacg attcaagata aggtatgtgc tggaaaaaat tagcatcgat
     3421 atcttcttgt tccaaagcta tatttggttg tacataatcg gtaaagatta tgattttgag
     3481 ttcatatccg gtctcttttc tgaagtcatc ctttacctgt tcaaggatct cattgtgcgg
     3541 aacaggggat gctcctatct tgaatacttt ttcttttgat gggttaagcg ttataacaag
     3601 aactactgca agaacaataa gcgcaacaag cagtgcaaca aacagacttc ttttttttaa
     3661 catggtacac ctcctaagta ttatgtagta atgatttttt aaataaaaaa atcggctaca
     3721 ccctgtgagt gcaaccgatt ttgattattg ccaatttatg agtgcaaaaa aaactcatgg
     3781 caaaataaag tcggttgcca tggcatgcac atatgaagtt ttccttttaa agtacagttc
     3841 attatctaac acctcggtat cgtatttctt ttttccaatc ataacatata aagtagttaa
     3901 agtcaatctt aaattaaaaa gaaaaagtat agttctattt tcagaatttg tatgttataa
     3961 tcatatatga atatttataa agtcatggag gaatgatcgg atgtttgaat tttttaacga
     4021 ttggcttgaa accaatggtg taaaaggact gttttcggat gttctgacca ccttatttat
     4081 aattggtata attgctttag tatcttatct ttcctataag atatccaagg ttattcttca
     4141 gcgtactatg aagcattttg ttgaaaaaag caagaccaga tgggatgatg tactgctcaa
     4201 acgcgggttt ttcgttcgtc tttccatgtt tttcccggct tttgtgatat acggtttttc
     4261 ttcggcattt gctgattatc aggatatcat acaaaggtgt gttttttctt atatgatctt
     4321 tgtttccctg ttgacgctca atgcattttt gaatgctttt accgatatgt attcctacca
     4381 tgaagtgtca cgtcaaagac ccataaaagg atatgttcag gtttttaaaa tattttttgc
     4441 cataattgct gtcataagta tagtgggaac ccttataaat aagccttttg gttaccttat
     4501 aggtggaata ggtgcaatga cggccgtatt ttctcttgtg ttcaaagata caattcttgg
     4561 ttttgtagca agcatacagc tttcagctaa cgatatggtt cgcataggcg attggataga
     4621 gatgcctaaa tatgatgcag atggtgaaat tgtagatatt tcactgaata cggtaaaggt
     4681 gcagaactgg gataaaacaa taacgactat tcctccgtat gcactgatat cggattcatt
     4741 taaaaactgg aggggcatgc aggcttcggg cggcagaaga ataaaacggt ccatctttat
     4801 cgatatgaac agtattaagt tttgtgatga gcagatgata aaaaaatttt ctgaaataag
     4861 atatatatct gaatacataa cctcaaaggt caaagagatt gaggaatata atagacttat
     4921 gggagcagac gacagtaatc ctgtgaattc aagacggttg accaatgtag gtacgttcag
     4981 gatctatata cttgagtatt tgaaaagaaa tccgcgcctt aggaaagata tgacgctcat
     5041 ggtaaggcag cttgccaata acgaaagagg tcttccgctt gaaatatatg cctttacaaa
     5101 tacaataata tgggtggagt atgaagccat acaatctgat atttttgatc atatacttgc
     5161 tgtactgccg atatttgagc ttaaattgtt tcagaaccct actggcaatg aatttaaaat
     5221 tcttcattaa aaacaaaaaa cgagccttcg atgaagtgaa tccgttttat tttaaatcag
     5281 gtcgtagcat gcaggctcta tggagatata caaaattact atccggagat tcacacagca
     5341 agcttacgcg tataatacca ttgatggagc cttcaaactg agcttcttta agacacatca
     5401 gtgaaacatg gtcaagtccg aaggctttgc gtgcaataga tgccgggttt ataaggttaa
     5461 tatcttctgt gactgaaaag gttatgctta ttatccgttc tactttattc cgccggaaaa
     5521 gttcttccat cagttccttt gtgcgttcag atatctcttg tatagaaaaa ttttcaaccg
     5581 aagtagctcc gcgtatatca acaatcacct tttccacctg agagtaaaat tttttctttt
     5641 ataaaggtta ggcaagcttt catgcttcct tcagaaacag atttccattt tgcattagaa
     5701 taaggtcttt ccagagaaca atgatcaagg gaaccgtcat agatgcttat ccttgcattt
     5761 atttcctgcg aaaatgtcag atagactctt cctgccctta tggaaatatc tgttatattc
     5821 tcttcaagct ttagttctgt acacaattgc ttaagcttgg taagcacatc tttttttgca
     5881 tatgcgtcaa gcttgaaagt cggagttgtc cttactgatt tttccatttt cttaccccca
     5941 ataaaaaatg atcataccga ttatacatca aatttattaa atccgcactt cttaaacttt
     6001 tatgataaaa tcttttttta taacaaagaa ggaataatta aaattatgtt ataatataat
     6061 tattattatc aacagggggg agaagtatat taaaaacatt cttaaaacag aatgatttat
     6121 caataatgaa aattcttttt tttctgataa cattatggct tgtttctgta cctttatatg
     6181 cagataaggt tgattcatat ggaaaagatc ttcacagtat aaagcttcag cttaagtggc
     6241 ggcatcagtt tcagtttgcc ggatattatg cggctttgga gaagggcttt tatgaacagg
     6301 cagggctaga tgtaagtatt attgaaggag gaccggatat ctttccggtg gcggaggtat
     6361 ataacaaaga agcacagtac ggtgtatgcg gatctgagct tcttcttcac aggctcaggg
     6421 gatatccgct tgtacttatt gcaaacattt ttcagcattc tccattggtt cttatggtcg
     6481 tagacaagtc cggtatttac tctcctatag atctcagggg taaaaaaatt ctgcttcatg
     6541 aggacggaag tgatgctgaa atatcagcaa tgctcatgtc ggaaggggtg gatcctcaaa
     6601 gctatggata ctatgacgga agcgtagata cagatatgct gcttgaagga aagattgacg
     6661 cactagctgt atatattacc aatgaaccat atcttttaaa aagtatgggg atggatataa
     6721 gagttatcaa tccgctgaag tacggtattg atttttacgg tgactgcctt tttacaacag
     6781 aagaggagat aaataactat cctctccgtg tacgggcatt ccgtcaggcg agcataaaag
     6841 gctgggagta tgctcttaat aatatagatg aaatagcctt gcttataaaa gagaagtacc
     6901 gcagcgatgc ggatattgat atgctcagat atgaaggcag tactatacgg gacagtttta
     6961 taatgaataa agaagtagag cttggccata ccaatcaggg acgttgggag gagataggaa
     7021 aaacttaccg ccggcttggc atgacagctt cgtcttctcg gatggagggt ttttttgcat
     7081 gggagtatga gaataatgag tctcccgaaa agttaccgtc caagctgctg ccttatatgg
     7141 gaattggcat tctgagctta ttggcaggag gtacgttgtt ttttcatata aagctgaaag
     7201 tgtacacaaa aaataagatt cacagcctgc aaaatttttt cagtctacct tcgtacatgc
     7261 tgcttgtaat ggattatagc ggaaagataa aaaattataa tcagtcctgg gcacttttga
     7321 tgaaaaggta tttcggaagt gaacatcccg aaaatattag acaggtgctt actggtgaag
     7381 atgtcggaaa aattgaaaag ttttgtcata tgaacgatcc tttacagagc ttaatatctc
     7441 tggaggtctt ttttaaaaat gcagaaagca gtgtctggat aaatctggac ttccatcagg
     7501 cttttgatga gaagctgatt tatggcatcg cacgtgatat tactgaggaa aaagcaagga
     7561 atgaaagaat gggagaaagt gtggaaattg ccatggaaaa cgcaaggaaa aagacagatt
     7621 tcatagccag catgagtcat gagataagaa ctccaatgaa tgcaatggtt tctataacgc
     7681 agatgcttat tgaatcaggt ttgagcattg aacaaaagga catagcggaa attcttatgg
     7741 tttctcagca caatcttttg cagataatca atgagataat agatttttca aaactggagg
     7801 caggcaagga tgaggttatt ctggacagct ttagcataaa agagcaggct aaaagcatat
     7861 atcagctttt tgaggttatg gcacgccaga aaaagttaga ttttaaactc aacctaatca
     7921 gaccgttgcc caactttatt ataagtgatt cgcaaaaaat cagacagata tgtataaatc
     7981 tcattagtaa tgcttttaag tatactgaaa agggtgaaat aattttcagt gtcggcctga
     8041 ctgaggcagg cattcttaag atatctgtcc aggatacagg cataggcata agcaaagatg
     8101 atattggcag gatctttaac ttttactccc gtatagagcg gaaaggaacc gttagacaaa
     8161 gcggaaccgg cctggggctt gtcataagcc aaaaatatgc gcgagccatg gggggagctg
     8221 taacagttga aagccaggtt tcaaaaggtt ctgttttcac cctcaccgtt cctgtgcatg
     8281 taataaatta acccgcaggc tggtctttca cctgtgggtt aatgattatc ctataagcag
     8341 agattcaaac tgtgcattat aaagattata gtaaactcct tttcttctta taagctgttc
     8401 gtgagtgcct tcttcttcta taccgttttc tgtaaggacg attatccttt tggagttgcg
     8461 tatggttgcc agtctatggg cgataataag ggtggttctg tcttttgaaa gttcttcaat
     8521 agagttttga ataatgctct cacttttatt gtccaatgag gatgtggcct catcaagaat
     8581 aagtatggga ggatttttaa gaaacatccg tgcaattgaa aggcgttgtt tctgtcctcc
     8641 tgaaagtttt attccccttt ctcctattat ggtttcatag ccataaggca ttgcagttat
     8701 aaactcatcc gcattggcct tgatgcatgc cagctttatc tcatcatctg ttgcatcaag
     8761 ctttccgtaa gcaacattct ctcggatact tccggaaaaa agaaagacat cctgctgaac
     8821 aattccgata tttttcctaa gcgactcaag acaaacagtg tttatatcaa ctgaatcaat
     8881 agttatactt ccgctgtcaa tatcataaaa tcttggtata aggctgcaca atgttgtttt
     8941 gcccgatccg gagggtccta ctattgcaac gctttcgcct tttttgattg tcaggttgat
     9001 atccttgaga acggtgttct tatcgtcata gctgaaagac acatctttaa agctgatttc
     9061 tccatggaca ttttcaagaa acttttcatt ggagagctct gtctccggct gaagcttaag
     9121 tatttctaca aatcttctga agccggccat tccttcctga tactgttcga caaatcttag
     9181 cagaatgttg agcggctgga gaaactgggt catataaatt atataagcaa caaagtctcc
     9241 gatgtttata cttccggtgt atacaaagta acctcctgca accatagcta tcactgtgct
     9301 ggagttcgag agaaaatgaa tggtagaatc gaagatgcct atctgcttta cggtttcttc
     9361 gcggatgttt ttcatttttt catttccctc atcaaaacga gtattttcga atctttcgtt
     9421 tgtgaaggat tttacgactc ttattcccga gatggactcc tcgatcctcg catttatatt
     9481 agcatagctt tcttttgctc ttctgaaggt gttctcaagt ttagtgttat ataccaccat
     9541 aaagtagaac ataaacggta ttataagaaa gactatcagt gtaagctgcc agtttatagt
     9601 acataaaagt ataaaagcgc ctataatctt aactatcgag agaaaaagat cttccggagc
     9661 atgatgagcc gtttcagaga tttcaaaaag atcgttggtt acccttgaca taagatgacc
     9721 tgttttggtg ttatcaaagt aagatatagg aagcttttgt atatgagaaa agatttcctt
     9781 acgcatatcg tactgtatcc ttagtcccag aatatgtccg tagtatccga ctatgtactc
     9841 taaaaccatt cttgcaaaat atagtaaaag taggaataat ccaaaggcaa agatctttga
     9901 aaggtttttg tccggtatta cggtattgat tatgtatctg gttaccatag gataagcaag
     9961 atcacttgaa gatattagaa atgcgcaaat aatatcaagg taaaaaagcc ccctgtgagg
    10021 tttgtagtag ctgataaact ttttgatcat aaggtcctcc gttcatacat taccattttt
    10081 tgaaaaaata atctgttggt tgttttttat atttctgaac tcttcaaact tatagataaa
    10141 agatgtaagt gcatcgtttc ctaaggattt gtaggaatta cacacggatt gcagtacttt
    10201 gaagttttca tcaggtatag ccgtttggaa cgatttttta ggtgttatgg atattcttag
    10261 gatatcttcc gatgaggctg ttccggaaag ctctatgagt ccctcaaatt caaagtaaga
    10321 aagaaattcg gttctttttt caatatcaat gcctgactga tgctttatat atgaaatacc
    10381 tgtaaacggt cttgcggaac tgttataaaa aaacttatcg caaagccaca taaaacgtat
    10441 aagccgtatg tagctttttt cgggaattat actcgtaaca gccataaaag acagtttcac
    10501 tctttcaata tcaataactg gatgggcttt aagcacaata gcttcataag gatttttttc
    10561 ttttacaatg ccattaaagg tttttaactt ttccagaatt tttttataag attccgaagt
    10621 cataagtttt ttattttttt ccaaaagttc aaagaaattt ttaggctcca aaaatgtttt
    10681 tattttttcg gagtcttctt ttttcataag atcacctgca gtatatttct tcagtgttga
    10741 gtgtggtatc tccataagga tcgaaagttc ttcaagagta cacataaaaa gtttctcgta
    10801 ctcttttaac tgctgtgaac ctgccaggaa atgctttttc tcatatatgc ggtaggcagc
    10861 aaaaagattt cgcagatata taacgttatt gtctatttca tttccacagt ttttacatac
    10921 ggcaaccata ccgctgaact ctatcggctc tgatctgacg gttatagtca ttttttttcc
    10981 ctgaaataaa aatcgttctt gacaccgcaa aggttacagt ataagttgtt ttccatttta
    11041 tatggcagga accttgacag tttcagtgat ctttttgtta agctccttca gttcaaagta
    11101 cataaagact gttgtaagca tggtcgagat tccatcggac atcggtacag cgtaccatat
    11161 tccgttgagt ccaaagaaaa gaggaagtat aataaccata ggtatcagta ctataagctg
    11221 tctgaggagg gtaagaaaca tcgatttctt aggtttccct attgcctgga agtaatttga
    11281 acttacaatc tgaaatccta taacaggcat aagaagagaa aaaattctca ttgccggaac
    11341 ccctatggag actaactcac cgtctgttct attaaaaaac tgtattatct gtgttgggaa
    11401 aaacatagct gacaaaaatc caagaaccac aaaaaaagtg gctccgatat aagcgtacat
    11461 aagggctttt tttactctgt catacttttc tgcaccgtag ttgtaaccta cgataggctg
    11521 ggttccctgg tttatgccga atacaggcat cataacaatc atggccgcac tgcttattat
    11581 gcccatggct gctatagctt tatctccgcc gtatctggcc agagagttgt tgagaataag
    11641 attatatacg cttcccacaa gctgtatcga aaacggtgca agtcccatag atactatctg
    11701 acgtacatac ttccagctaa gtttcatatt ggctgcatgt atctttacgg tgcttttttt
    11761 tgccataaag taataaagta cccatgtcgc agatactgcc tgagatatta tggttgcaat
    11821 agctgctccc tgaacgccca tcttgaatac aaatataaac aacggatcaa ggacagtgtt
    11881 caggactgca cctattacca tggtactcat agctcttttt ggattgcctt ctgctcttat
    11941 aaaatggttc attccgaaac cgatgaactg aaatatcgaa ccataaagta ttatgctcag
    12001 ataatccctt gcataggcta aggtattctg gctggctcca aaaagaagaa gaattttatc
    12061 aaggaacaaa agaaagacca ctgagtaaat cagcgaaact ccaatgagca gagtaaatgc
    12121 atttgcggca attttttctg cttcttcttt ctttttttct ccgagtttta tggagataag
    12181 agaggttgca ccaaggccga tcagcattga aagagccata gatatgagca ttataggaaa
    12241 agttactgtt aatccggaaa gtgccagaga accaactccg ttgcctataa aaatcctgtc
    12301 taccacatta tacagagcat ttaccagcat ccctattatt gccggtatag aaaattttac
    12361 aataagtttc tctatgcttt cttcacctaa aacttttgaa tgatccatgc ttacctccga
    12421 aaaatagtct tatcataatg gtactctaaa agtatatttt ttataagctg tttagtttaa
    12481 attgcgttaa aaataaggga gagagtatct cccttaaagt ttaaaaccgc tattttttga
    12541 aggttgaatc ctttggaaac agcattatag tcttcatggg atctatacgg cttatggagc
    12601 tcttatatat aagagtctgc tgtccgtctt tttctatcag cactgtaaac tcatcaaatg
    12661 actttatgta acctgaaata ctgaacgttc cttcaaaaaa gatcttaagt tctatgttgt
    12721 ttactctggc tatgttcagc agcttgtcct ggattttggt tttttctgcc ataaaaggaa
    12781 cctcctaatt ttcttcatta ttctgagttc ttctgatata tttgtaaaac ggtttttcaa
    12841 aaacgacttc atttttttgt ataagttcct taagcaggct gatgcaggtt ttttcatcct
    12901 gattatcgaa cacatactct gagacatact taatatcctg tgaatggata tcttcatctt
    12961 taaggatcct tttaatgtaa aaagtatcca ttgaagcttt aagctgttca aaactgcctt
    13021 ttattatgac gggagtttta atgctaacat aatcgctgcc gtcttttcca aggaacatac
    13081 ctgaaaactt aagggcgata ccgaaagagc ttatagcttt gtttgtaaga ccaagagaat
    13141 cctttgcctg atgaagaacc tcgtatatag tgtttgactt tttctctgca aacgatttga
    13201 aagtcatatt tacgaagttg tttctgtact ccgggtatgg aaggagtttt tcatacttta
    13261 tctcccatct tcgtggagcg ggtactttca ggattccgga atctgttttt atcagtctct
    13321 tagattctat catatttttt atcaaatctt tcaacgatgt tcttgaattt gatgatatcc
    13381 acggatggcg ggatatggca gtcgattcaa gagatttgta gtcaacgggg gttatggatg
    13441 taacgataag tctttccatg aaacgcatgt agttctcttc atattcactg acttcatcac
    13501 tgttcataag cctgcacttt atagctttca atatatcttc tacaaaagca ttcatggaag
    13561 tatacctgaa ttttgaatag tcaacgccga tgccggtaat aattcttttt acgattgcct
    13621 catcgggaga aagatttttt tcatcagtct ttcgtattat ctcttcgaca agatcatcaa
    13681 aggtatctat atccactata gattcatttt ttacaatatc ctcgtatgga atatacatat
    13741 cggctgccag ctgcatatct gcggatgtgg ttttgctgcg gctgatgaca attacagatt
    13801 tcccgtatgt tttgagtttt ctcaccagtg gcagaaagtc tgcgtcccca gtcacaagaa
    13861 cgtatgattc tatatgcggg aacatgaaca tagactctat tgtatccaca gcaagtttga
    13921 tatcgtttcc tttcttattt gagaatccat cttccggcat ttcgatcagt tctataccgt
    13981 actttaaaaa cgtgaacatg gtagccggat atttggacca gtgtccataa gcctttcctc
    14041 cgataacttt agcaaattca gcaatctttt ttactataag ctcaggatca accggttgat
    14101 tctgatagtc cataaggatg aaagaagttt tcattttttc ctccagtata aaaatgcgtg
    14161 gcatagccac gcatgaatta agagttcatg gttttaagag tatgggcaca tcttggacat
    14221 gtatctggga actgaggatc ttcgcctgta tgttcatcaa ttttccagca cctttcgcat
    14281 tttttgccgc tggctttctc tactctgacc ctgcaaagac ttccttcata tccgttttca
    14341 acagtacctg tttcaaaccc tgaagcaatt atgacatcag acataaattc atttcctaaa
    14401 gaatcaagta tagcacaatc tgaaggttct ttaggctgca ccgtaacctt gctttctaaa
    14461 gagtggccta tgaacttatc acgcctcttt tcttcaagtg ctttgagtat gtcctctctt
    14521 atgagaagaa ctctgttcca cttttcttcc aatgctttat caatatagtt ttcattgaat
    14581 tcgggccaac tttcagcctg tacagtagcc tttttttcaa agttttctga gagatggtcg
    14641 tatatttctt ctgttgtaaa ggtaattatt ggagccatca ttttggtcat ggccatgagg
    14701 atctcataca ttacgctctg agccgaacgc ctcatttgag actttttgct gagagtataa
    14761 agtctgtctt ttatgatatc aagatagatg gaactcatag aaacagtaca gaagttgtta
    14821 agcatctggt gtaccctgta gaattcatag ctctcatacg cctgggttac atcacgtata
    14881 agctcatgaa gcttcatcat agcccagcgg tcaagttcag gcatttcttc atacttgaca
    14941 agatcttttt ttggatcaaa gtcgcttata ttgccgagca aaaaccgtac ggtgtttcgg
    15001 aactttctgt aaacatcaac ctgctggtca agtatcttgt aagatatgct tatatctccg
    15061 cgataatcgg aagatgcaac ccaaagtcta agcacatcag ctccgtaacg gtcgataatc
    15121 tcctgtggtg ctataacgtt gccgatagat ttggacattt ttttgccttc ctcatccttg
    15181 atgaatccgt gggtgagaac ctttctgtaa ggagcttttc cgtgcttccc aaccgacata
    15241 aatatcgaac tctgaaacca gcctctgtgc tggtcgcttc cttcaagata aagatctgct
    15301 ggaaattttt taagttcttc tctggcatgg ataacggcct ggaaagaagc tccggaatct
    15361 atccatacat caagtatatc gtactgcttt ctcagatgat ccgatccgca tttgggacat
    15421 ttgaacccat ccggaagaag ttccttaaga tccatatcgt accatgcgtt cgtaccttta
    15481 gcttttacga ttgaaataaa cttttcaaga acctgtggat caagaagtac ctctgtacag
    15541 ttttcacaat aaagggcggg tataggtatt ccccatgcac gctgtcttga tatacaccag
    15601 tccggcctgt catgaatcat ggaggtgatt ctggtttctc cccattcagg tatccactgg
    15661 acatttttta tttcattcaa aactttctga cgcaaactgt tattttcaac gcttataaac
    15721 cactgctcag tagctctgaa tattatcggg cttttgcatc tccagcaatg cggataggaa
    15781 tgagttatct tctggcttgc tataaggtgg ttgctggctt ttaggtcatt tattattact
    15841 tcattagcct tccatatctt aagaccctca tactttccgg cttctttggt aaatataccc
    15901 tcgtgattca caggggaaag aaccggtaga ttgtacttca gaccggtagt atagtcgtcc
    15961 gctccgtgtc cgggagccgt gtgtacacag cctgagccgt cctccagagt cacatagtcc
    16021 gctaaaacaa tgagagactg cctttcaata aaaggatgtt ttgccgattt gttttcaagt
    16081 gaagaaccct taaatacttc tgttatttcg taagaatcaa ttttagcatc tttcataacc
    16141 ttaggaacta actgtttggc aagtatccag tattcgtttc ctacctttac cttgcagtag
    16201 tcgtactccg gatgaacagc gatagcaaca ttggccggaa gagtccatgg tgtagttgtc
    16261 catattacta tataggtatt tacatcaaac tcaaacttca catatataga tgaggaagaa
    16321 tgatcatgat actctacttc cgcttctgca agtgcagtct gacattcagc acaccaatag
    16381 ataggttttt tctttctgta aacgtttccc tgctttacaa aagagttgaa tacatcaagc
    16441 acttgtgatt cgtatacagg atcaagagtt acatatgcct catcccaatt tccgcgtacg
    16501 cctaagcgct taaactcttc tttttgtatc tgaacgtgtt ttagtgcgta ctccctgcaa
    16561 agatccctta tctggttctt gctgagctgc ttggcctttt ctccgagttc tgtagttact
    16621 ttatgctcta taggcagacc gtgggtatcc catcctggta catacgggga gttataccct
    16681 ctcagggttt tatattttat gactatatct ttaagaactt tattcagggc atgccccata
    16741 tgtatatgcc cgtttgcata aggaggccca tcatggagca aaaacatctc cgagtctttt
    16801 ctggtctgct gaacatactt ataaatatct aaaccttccc attttttaag aatttctggt
    16861 tccttttcct taaggttggc tttcatggaa aaagtagtac tgggaaggtt caatgtatct
    16921 ttgtaatcca aaacaaacac ctccaggtag tattaaagct ttaacagatt ttgtatggta
    16981 tctttcagtt cggtgagatc catcgatttg accacataag cgtcggctgc ccatgaagcc
    17041 atttcgctct tataatgtga gtaggctgta agaaaaatga tcttcatctc cggatatttt
    17101 ttgcgtatat cgccggccag ttcaagtccg tttatatccg gcatttctat atcggtgcac
    17161 acaagatcga agtttctgtc cgcagaaagt atctgcaagg ctttttttgc gctgttggcg
    17221 tcaacaattt cataaccgcc gtcaagcagt tcttctttta taaggagcct tatgttatcc
    17281 tcatcgtcaa caatcagtat tctagccatt tagaccgcct ccttaagcat tatgattatt
    17341 ttcagcatga atgcaattca atcctgaata cagtactgaa atcatcagat tttaacaaaa
    17401 agagttttcc tttatgttcc tcttctatta tttttttaca tatagccagt cctaaacctg
    17461 ttccgttggt tttggtagtg aaaaacggca gaaaaagctt ttcttttatt tccggcgtta
    17521 tagctgttcc gttattttca acctctataa caatggttga atcatcagcg aaagagtgta
    17581 cttttatgct tcctttttgc ggaacaactt caatggcatt ttttacaaga tttataagaa
    17641 cctgcttcaa acggcttttg tctccgttag tgaacaattc tggttcattt ttatgtacct
    17701 gtatatttat ctccttttgg gagataaaat cctcgaacat cattacaaca tcgtccaaga
    17761 tagtacctat gcttactttt tcaaaagttg gcagtctttc acctcttgag tactctaaaa
    17821 tttctgaaac tatatcctca agccgtgtaa gttcggactg aattatgccg gagtacttcc
    17881 ttatgctctg cggatcatca agctttttaa gcatacggtt aagaaagcct cctatgacgg
    17941 taagcgggtt tcttatctca tgtgcaattc ttgctgtcat ttcacctatg gcggaaagct
    18001 tttccctttt ttcaatctcc ttctgaagtt tgtaaacctc cgttatatcg ccaaaaacga
    18061 ttattactcc ttcgattact cctagatccc tgttccacag aggtgaaaat tctatatcga
    18121 atattttttc ttcttcatga acattaagtt tgtagtttgg aaacctcaga tccgtcttag
    18181 tgtcatagat ctgttctatg gtgtttagta tatcgtcatt gaaggttcct gacaacagct
    18241 ttatagaagt tccgataata ctctctctgg ccttggaaaa aaatacctct gctttctgat
    18301 tccattcgag tatctttcct gttctgtcaa ccacgactat cccgacagaa agattctgaa
    18361 gaatattatc cttaaacctt cggtaataat ctataaggtt tttctgttcc tcaagcgatt
    18421 tggtcttttc tttaagctca tcgtagtttt ctatcatttc caggccaagg cctatgctgt
    18481 cctgataaag ctttatgatt tcaatatcca catttgtgat gggagtgcct gtgaacttat
    18541 tgtcgataat aacaattcct cttgtatcaa atcttccagt tacggggaat atcaggaact
    18601 cctgtgtccg gataagttcg tatatatctt caagctcatc atgttttatt tttaacatct
    18661 ggggaaccac atgtattacc tgttttttgc ttacaactct gtcaaggatg ggatgtccct
    18721 tatataccag aaccatattt ttaacgtttt cagtcagctt atttgtaaaa ggaagcgata
    18781 aagcttcttc tcgcaggtac tgtatcatat ccccgtactt taaggccctt ctgtttgctt
    18841 ccttccagat cttatcgaca tcttcgtctt catccggacc gatccaaagt ttgggtatca
    18901 gaaaccccct tacacggtcg cattcaagat agagtgccct gttgaaacgt agtcctcttc
    18961 ctgaagtcag accaagcagc ataattttta tagagttgtt cttatcgtat gttgaacgta
    19021 tagcctgact tattttatga agtgagttca tcttgtcaag ctgctctttc tgaaccctta
    19081 ttgactcctc atgcctttgc ttaagctcct taagattctg aatttcattt ttcagtctgt
    19141 tatagatatt tattctgctc aggcccagac ccattcgttt gactgtactg ttaagaaata
    19201 tctggtcgat gctgtcaaag tacttatatg gaagaaaatg ttccctttct tttttattat
    19261 atatggcaac tatcccgaag gtaacgttgt ctacggaaag tcttgaataa actgctgact
    19321 tcatatggat atcaaaaggg ttttcgtatt tttcaaagtt ggtattacct attaaaagca
    19381 gactttcttc tccattcaag aggctacctt caacagaact gccgatagga agaatatttt
    19441 ttttcacttc agatggttct aatccagaaa tattgtaaag tacaagtttt tcatcctcat
    19501 acttccagta ggcagctact tctgcaaaaa gagtatcctt tagaagacaa agaatattat
    19561 ttataagagt ttcatctgac tgagagttat caaaaatatc tgataatttc tcaaaaattc
    19621 ctaatttttc tctgaaggca tttaccgagg aagtatccac aactaatttc agtacgacaa
    19681 gataacttct cagagcctcc ctgagatttt cagcctgaat gtactgctta ctcaaaacaa
    19741 ccgctcccat gaatgactct cctgaaaaaa taggataaaa gaatacagtt ttttcctctt
    19801 tcagggtgta gattgaaaaa aactcattaa agcccagttc cactatattg ttttttttca
    19861 gttcctcaaa gagggaagga aaatcattat agtatacttt caggtcatca tccctgggat
    19921 ccatgtccat agatgagcct aagagcttaa gaatattacg ttcattttca aatataaaaa
    19981 taaaagaatc tatcccaaac ttttccctaa gtgaaagggc gatttctttt gtaatgcttg
    20041 tgaaaaagtc tttaggatac tgatgttcaa taattttcag aattgagtct atcatcgtaa
    20101 tcagtccctt cattgcaaaa ataagttaat tatattatac tatatttggt attataaatg
    20161 tacaacaatt agttttataa atactatgtt aggttgtatg aaaatggaat aaatcttaaa
    20221 actaccggag gaaaaatgtt taatatatct gtaatagtca atgccgttgc gattatagcc
    20281 ggcggaatcc ttggattgct tataggtaaa aagctctctg aaaaaattca aaagattctg
    20341 tttacaagtg tggctctcac cacaataata atgggagtac agatgggact taaagccgaa
    20401 aattttcttg ttgttctggg agctctttcg ataggcggtg ttgccgggga agtcattgat
    20461 attgagggac gtctggccag acttgccggc agaatcgaaa agtctgacgg tcaaactaag
    20521 tttgtaaaag gcttcgtaag ttcaacggtt ctttttgtgg ttgggcctat gactgttctt
    20581 ggctgcataa atgcaggcat aacaaacgac aactctataa tatatctcaa atctcttctg
    20641 gacggaatat catcagttat atttggttca gtatacggtt ttggggtaat agcctctgca
    20701 gcgagcgtta tgattatcca gggacttttg gtgagctttg ccggttcttt aagcttcctt
    20761 tctgatgaag cttatctgct gaactttaca gcggtgggcg gggtcatggt tctggcaata
    20821 ggaataaggc ttctggatat aaaagaaata aaggtaggaa acttcctgcc ttcattaggt
    20881 gcagtaatac tgctagacta tataaaaaca cttatagctt gaaagaaaag aaaaactcac
    20941 ttataagcta tactggcgat tatggttaca atgtttccgt cctttgaaat gtgtaccttg
    21001 acatcctttt cattgacgct gagatttctg caggcataat cctttatgta tgtgactatc
    21061 gactcaattt cttcttcgga tttgttttca aactttatag ccgtggcaca tgcttcgcgt
    21121 ctgttggtaa caaggtcatt catcctcttg actgctatat ctctgtttga atcttttttt
    21181 gcaggagacc accatgccac aggaatcacc ttctccccag tagctttttt atgaacccca
    21241 ggaatccatc ttccgaggag cctgcgatat cttcactaat gggtatcgga tttcctaaaa
    21301 ttctctgtgc aatgttttta aaaggaatag atagtctgtt cctttcgtca agaaccaacg
    21361 gattgccttc attggtagac acgattattt cctcactgtc aggtactact cctaacagtt
    21421 ctacagcaag agcatttttt atctcttccg gtgaaaccat cttatctctt tgaaccattc
    21481 tgggtttaag cctgtttaca ataagcgaga ctctctgttc cgaaaacccc tggttttcaa
    21541 gcatgccaat taccctgtcc gcatcgctga tagcagtaag ctctggagtt gtaactatta
    21601 tggcctgctg ggcaggagca atggagtttt tgaagcctct ttcaatacct gccggacagt
    21661 caattattat gtagtggtaa tgttttgaaa gttcatttac aagatttatc atatcctgtt
    21721 ctgataccat atccttattg gcaatctgtg aagaagccag aaggttgagg tttttgagga
    21781 gcttatgttt tacaacggct tcaagaggat ctttatttcc acgaacaacg tctattatgg
    21841 tgtaaactac cctgttttca agtccgagga caagatcaag gtttttcagg ccaatatcag
    21901 cgtctataag acagacatta tagccaagaa gggcaagaca tgctcctacg ttggctgtta
    21961 cggtagtctt accgactccc ccttttcctg atgtaaccac aaataccttt gagtcatcca
    22021 tctcttccgc ccccaatttc aattattatc aagtcaggtt tccaagtacg cacaaagtaa
    22081 ttatacctta agacttcttt ttttacaaat acaaaaggtt aatccaggtg tttttatttt
    22141 aaagtacaaa aaagttagcc ggacgataaa cgcccggcta acagttaaag aattactctt
    22201 cgattgatat tgcctgtact ggacaagaat ctgcagcatc ttttgcgcaa ggagcatctg
    22261 tctggggagc aatagcttca gcctttccgt catctccaac tttaaaaacg tccgggcaaa
    22321 gattttcaca cactccgcag ccaatgcaag cgtccttatc tacaaagact ttcatcaata
    22381 cacctcccga taggatatga gaccatttca agtccaaaaa gtattatacc acactatata
    22441 taaaattcaa aatcagaaat ttttaacctc ttttacaata cggtctacaa tgtccatgcc
    22501 tactctccac tgagcttcaa cagtagcggc gccaatatgg ggagtaagta tcacattagg
    22561 cattttaaag agtttttgat atacttctga tttgggaggc tcttcttcgt atacatcaag
    22621 tcctgcacca aagacttttc cgctttccag tgctcttaaa agggcaggtt cgtctattat
    22681 gccgcctctt gatgcattga caattataac gcctttcttc atcatttcga actctttatc
    22741 agaaaccata tgggtcgtct tttcagtttt tgggacatga aaagatatta tatcgcagtt
    22801 tgaataaatt tcttcaagtg atacctgttt ggcattgtac tcctttaaag tttcctgggc
    22861 tatctcaaaa acatcgtata ccattacttt aagattaaag ccggagagta tctttgaaag
    22921 aagcttaccg atatttccaa aacctactat accaagtgtc tttcctgaga gttcaaaccc
    22981 ctcaagactt tttttctccc actttccttc tatcatgcca gtggtagatc ttacaaggaa
    23041 tctgtctata gaaagtatca tccctgctac cagttctgca acagaaagag agttggcgcc
    23101 tggggtattg attactttta tatttcttgc cttagcggca ttctgatcaa cgttgtcaag
    23161 acccatacct gctcttgcaa taagcttaag atgggttcca gcatcgatta tttctttggt
    23221 ggccttggtt gcgcttctta ttacaagaac atctatttct ggcatcttgg ctataaggtc
    23281 tgccgtctca agatgctgag aaattatagt atgtcccgga agaagttttg aaagtgtctg
    23341 ggtagcctgc ttgtccaaag gatcgtttat atgtataagc atgctatatc cctgccttca
    23401 taaagacttc ctgagctgct cttactcctt ttccgagttc aacattgtat ccgaactttg
    23461 caagacccat ttcaagggca gcaatagcag ttattatatc aaactgtgac atatagccga
    23521 gatgagctat tcttgctatt tttcctttta catcgtcctg accgtcggca aaaactactc
    23581 cccaatcgtc tcttagcatc tttataatct tggaggaaga tatgttttcc ggcatacata
    23641 ctgaagtgag aacgtttccg ggatccttgg cgtaaagctt aagtccaagt gcttgaacgg
    23701 cggctcttgt tgcctgggca agaagctcgt gacgtgccca gatgttttct ataccctctt
    23761 ttaagagcat ttccacggct ctctcaagct gatagataag atttatcgga ggagtccatg
    23821 gtgaatccgg gtagttcttt ttatactgcc taagatcaaa gtagaatctt ggagttttac
    23881 agttttcaac tgctttccac gctctttcgt taagtgagat aagagcaacg cctggaggca
    23941 tcataaaacc cttctgaaca ccggtaataa cgacatctac tccccattcg tccattttga
    24001 gttccattgc gagcagaccg gatattgcat cgacaactat cagagtatca gtatttcttg
    24061 ttatagcggc taactccttt acaggctgcc taccgccggt tgaagtttcg ttaagctgtg
    24121 tgaatacaat tttggtatcc ggatgttcct taagcaaagc acttaatctt tccggctgta
    24181 ttgctactcc ccaatcaagc ttttcttcaa caatatttgc accgaaagtt ttgcacagct
    24241 tgagccatct ttcgccaaac ttaccggaat taataacaat taccttatca ccgggattta
    24301 caagattgga aactgcagtt tccattgctc ctgttcctga agaagacata agcattacat
    24361 cgttgctggt ctgaaacaaa acttttgccc cttcgagagc ctttttctgt atttcaagat
    24421 actgtggtgt tctgtggtga attgtatcct gagcgccagc caaaagaata tctatcggta
    24481 ccggagccgg tcctggagtc atcaaatagt ttttcctaac catcttaatc acgataaatc
    24541 cctcctatga aaaaatattc aagaaaaaaa tttcatattc tcaagttcta ttttacatta
    24601 ttaaggacat aaaataaagt cggcaattta aagaaaaaaa tataaaacct ctgataacta
    24661 ttaaatagtc cggttaatta agaataaatg attaaaatag aagaaaaatc agaactcagt
    24721 acgggaaaaa caagtttgtt tcacaaatca aaaatactta agagccatta gcataaaata
    24781 atatgtcctt tctgtttata gaaaggacat atggcacgcc cggaggggct tgaaccccca
    24841 gcctgtgatt ttggagacca ccgctctacc aattgagcta caggcgtatt caaagacaga
    24901 aattattata tcatataaag ctcgaaagtg caagtactta tttgcttttt ttaagcattg
    24961 ctacttcata tatcataact aagaagcata aaattccgaa aaaaactgtc cagagatctg
    25021 ccgaaggtaa aactgacgga tcaacaaccg ttgaaagaaa ggaagataag tatttaaaaa
    25081 gttcataagt cccgatcaag aaaccgatca tactgcttat tatgatatgg gcagatttat
    25141 gataccatat cggatcgtag gcaaaaaaac cgtacacact tgtaagaaaa acggtaaaaa
    25201 agcctattga aatgaatata tcataagtga acttaaaaaa tgcaagtgtt attgatactg
    25261 tcataataaa aataagtaca agattttttt tcacagaatc aagcctccta tgaaaaacac
    25321 tatatttgca agtatataag cagtaaatat cgtatatatg aaggtgaaag cagcccattt
    25381 atatgaagcg gtttctagtt ttacagcgct caatgtggca aaacaaggaa tatatgccat
    25441 tataaaatag agaagagcta acgccgactg aggggacatt gaagatgcaa gagcatcctt
    25501 aagtgcggca ggatctgtat taagtgttgc tgcgtacgtg gaaacgatta cttctttggc
    25561 ggctactccg aaaaccaaag ccgtgtttgt atgccagtcc catccaagat gtctagttag
    25621 aaattcaaaa gacctgccta tggttgcggc aaaactgttt tgtatatcgc ttcctgatgg
    25681 aaaatacgat aaaaaccata ttaaaatgga agcaatgaga attattccgg aggctttacg
    25741 caaaaaatgt tcagttttct cccacgtata aaggaaaaga tttttagccg tagggagtct
    25801 gaatcttgga agctctattg taaacgggat atcatcggtt ttggttacga atacctgcaa
    25861 aagtttggca gaaagaagca caagaaggat acttgaaata tatagcagaa acattataag
    25921 tgctgcatgt tttgggaaaa aagccccgat aatgattata tatacaggaa gtcttgcact
    25981 gcatgaagcg aaaggtatac tcagaagtag ggccagacgc tccttagtgc tggatatggt
    26041 acgtgtggac attatagcag gaacgttgca tccaaaacca agtattattg acatgaagga
    26101 tctgccggaa agaccgaatt tcctgacgat cttgtctaca aggaacgctg cccggggaag
    26161 atacccggac tcttcaagaa agcccatgaa aaagaagagg acaaatattt gtggaatgaa
    26221 ggcaagaaca ccacctactc cttcgattat acctgttgta acaagagatt tcagccatgg
    26281 tacggaaatt acagaacctg cccaagagga aagtttacta aaaaaaagat tgagaagatc
    26341 gcttaatggt gtggctatgc tgaatgtcat tgagaataca aagaaaagtg ccacaagata
    26401 aatgagaagg ccaagaactt tgtgcgtaag aacatgatca agaacatctc tgagtgacca
    26461 gctgcgtccg aggctgacta tgctcatgtc agtaaggttg cgtatcagac cgtacttcca
    26521 gttcagatag atatccttga gttttttcat gtcaaatgag gtgcagatac tttgtgaaag
    26581 ctcagcctcc gtgtggatgg atcctgctcc gaactcaagg aaataaatgc ccagccattc
    26641 atggtcaaaa ccagaaagtg aaggatactt tgcggcctta gacttaaaag tctctatata
    26701 ttcgtttacc tcaacgggat actgaaagtt aatatcatga aattttttct gctcagaagt
    26761 tctgtgaata gcctcaagca actgtgggat acctttcatg gtctttgcag atgtgagaac
    26821 aaccggaatg ttaaaagttt tagaaataac ttccggattg attattcttc ccttttcttc
    26881 ggcttcgtca atagagttaa tagccaaaac cgccggaagg ttcatctcaa gaatctgtat
    26941 catcagatgc attgaccgtt cgagattaag tgcatcggca actattataa ctgcgtcagg
    27001 tgattcgttc aaaaaataat cccttgcaac cctttcgtct tcgctgcatg cggaaagagt
    27061 atatattccg ggaagatcga ccaatttgaa gttgtgacct ttgtatgaaa atgatccgat
    27121 ctttttttct acggttaccc cgggccagtt agcaacatac tggcgtgttc cggttattat
    27181 attaaacaag gatgtcttgc caacgttcgg atttcctatt attgatactt caaatgccat
    27241 agatcaccgc tttcagacat attttttaca taaaattttt ctgcttattc ttttacccag
    27301 aagaatatcc ttttggttag tcctgaaaaa gatttttcct ttgtaaagat gtttttctcc
    27361 tgtgattcca ggatagatac caagactttt caatctttca taaagcatca ttccggaatc
    27421 tattgtgaaa acctcacaca ttccgtcggg tatgctgaat aaagtttcaa tcaggaatcc
    27481 tacatctata agtattttgc ctgcctgttc cctgcgtatc ataattttat tatcgtcaat
    27541 aagaaaaacc ataggatcgc caaaaggcga aacatgcacc agctctatat ctttgccagg
    27601 aacaaatccc attgccaaaa accgttcttt tatctgttcc tcaaagtcaa ttgactgtat
    27661 ctttcctttc attcctactt tcagactgct caaaggt
//
LOCUS       DAOSHR010000004        26426 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_1449_length_26426_cov_5.937773, whole genome shotgun sequence.
ACCESSION   DAOSHR010000004 DAOSHR010000000
VERSION     DAOSHR010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 26426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26426
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1449_length_26426_cov_5.937773"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="PLU28_00405"
     CDS             <1..230
                     /locus_tag="PLU28_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG33553.1"
                     /translation="LFFMVLSVSLMGLFELIVSNRSFPVLAYGSYRNIFHYFVLNMTS
                     KTIIEVLFFTVITVFFLFLSVSIFKQREFNA"
     gene            complement(302..2149)
                     /locus_tag="PLU28_00410"
     CDS             complement(302..2149)
                     /locus_tag="PLU28_00410"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HOG33554.1"
                     /translation="MSKEQSLKKMISKRMFWIFLIITFFTAVVLWLFLNVFFERNVYK
                     KVYDQSVANIYRTIRNSHKKSILDDITRVKESSFYIKEISIYDPNLLPLYSWKGLKDN
                     EKFVFKEVLDTSFPQEISMAGGIRSYYSVCHIMTDNGQEEKYFIKIELDFSFLDNLRI
                     LLTLFFFGAVIAVSIIITFAMNKTVNKVIKPFSILSSNMQEFAKTKVYVVQPGIESSQ
                     VKEINMLVQTYNEMANELSANIQELRAINEELENSYSETQSTNEKLEKIINLGIDLSN
                     AVIVSDREFLKKVLSLSKELVPQADYGYVYLIEDGEWVFIDALGHNVDDLNDLSLKKE
                     NIPDFQKILLLENKEFINEKTVSQNTLQTYLRVKKPCGQAMVIKLAVGSNDAGGLCLE
                     ISRHKNIKFSRDASKVLETLASFASAFLTMQRFSSMQNEFQRQIIISMVSILEIYDNY
                     TKGHSDSVANCSLKIGARLGLTQKQMKDLYWSGLLHDIGKILVSPDIVNKPGRLTIQE
                     YEAIKKHPVYGYEVLNKSDQLKDVAVYIKHHHERWDGNGYPDGLKEEGIPLISRIIAI
                     ADSWDAMRTDRSYRKGLSTSQAIKEMGENSGKQFDPEILEVFLRMMSEE"
     gene            2453..3547
                     /locus_tag="PLU28_00415"
     CDS             2453..3547
                     /locus_tag="PLU28_00415"
                     /inference="COORDINATES: protein motif:HMM:NF014381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD/NADP octopine/nopaline dehydrogenase family
                     protein"
                     /protein_id="HOG33555.1"
                     /translation="MRIAIIGAGNSGISMAAHLALSGECVNLWNRSAQTIKDLVPTKS
                     ITVQGKVNGKAYLNIVTDSIELAVKDAEMIFVTTPATSHAQIAQRLAPYVDKNVPIIL
                     TPGRTFGAIEFKHILLQNGYSGNVNVCETQTIIYTCRKTDSCNAVILDLKKNVLISCL
                     DPSKIESIVSSMPQCLKKIYKPTKSMVETSIGNVGMILHCTPTLLNSGWIESPSHSFK
                     YYYHGISPSISSFLERLDEERIHISEVLGNSVESTYQWMKRSYGVEGETLFQCIQNNE
                     AYKEIDAPDTLEHRYILEDVPCGLVPLESIGAKLGVKTPYVSLIIDLANSMLKKDFRS
                     EGRTLEKLGMGSMLPEQIITILNHQELYSL"
     gene            3544..5445
                     /locus_tag="PLU28_00420"
     CDS             3544..5445
                     /locus_tag="PLU28_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG33556.1"
                     /translation="MNGKRVFGFVKPAIDAHSLGMFSLAEIIKECGYTVVLADTEINS
                     ALENLSSKKNLSVFLRWLKNNRITDIGFSYRLDPNDGFSLFSNMMFFLIKNRMLRTGG
                     KSIQNIYYAGLAEGCRKVEQEFGRYAKVFIGGESISETLNILSVDSSSIPPQMRESCR
                     YDNFLFDFSRDIIKKGEYTSFKPVERNGYGGYEGYGTKKDTLLKRLEHSQKNNLPPII
                     RAHAGPYLQEREKAVKLFYEWCEELGKAGMLDVLSIGSSQLTQSHFGQDWQDLPNGGG
                     VPVNSIQEYSKIWESSRPMLVRTYAGTKDLLKLAMMYEDTINIAWHALSLWWFSQIDG
                     RGGNGVYENLKEHIQTIQYAALTNKPFEPNVPHHFAFRGADDVTYIVSAYLAAMTAKK
                     NGIKHLILQNMLNTPKYTWNIADLAKSRAMLELVRSLEDEDFKVTLQTRAGLDYFSPD
                     PEKAKIQLGAVTALMDDIRPQDPHSPGIIHVVSYSEGYGLADPQVINESIKITLHTLE
                     EYRKLKKTVSVMKEFGLSEIDQRKDSLLQSAKKIISTMQEKIPNLYSASGLYTAFACG
                     FLPVPYLYLQKEEFEYARAVKTKIMNGSVVKTDEYGNTMTDEQFLDYAKSNISKIQIQ
                     SLMRWGDNF"
     gene            5442..6374
                     /locus_tag="PLU28_00425"
     CDS             5442..6374
                     /locus_tag="PLU28_00425"
                     /inference="COORDINATES: protein motif:HMM:NF014686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOG33557.1"
                     /translation="MKIAVVYDTDTYEQKKNMVTAVKNALLKKYDATELAFDENFMYE
                     IKKYDMVFNLSSSGGRESRQMHVPAILDIMGILHTGPGSYTHALCLNKTVTKIILRQN
                     GIPTPLEIKQQDVFAHRWEEGTCFIVKPEREGSAKGISDKSVVYDAQSMKKQVEYINH
                     TFKQPALIEEFIMGRELSIGIVGNDESMEVLPILEIDFSGLPQEIEHFYSHRVKEEYG
                     HLTSYKCPASLDNKTERVIKDYAAKIFNVLELKDYCRMDVRLNEKNEPFFIDVNSMPM
                     LMPDYSDIIKMAKAASLEYDDLILKIAQSALSRK"
     gene            complement(6395..8167)
                     /locus_tag="PLU28_00430"
     CDS             complement(6395..8167)
                     /locus_tag="PLU28_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HOG33558.1"
                     /translation="MRSVIINGEVSYSAIAQIINSNVFEQILSELISDSQERKNSLHS
                     FFELFLKNTDEVDPVKKYDISAIIQLLLALTINPIEKIDGSPYYNFPKMGNRKDQLVK
                     FIEGLFNLWRNKHRFMYKTDKYTSESFDRIFKQMLLVNTNSDLKNIVQSMYRQILINI
                     SDNRLKILRQVPSSAQVGFMIDKPEIKENSKIKNAGWLYDMNFIWSIVFEPPVIFYTR
                     SNTRKGLFKVYDQPILESIGLKNTEDWFVFPIHVASKFFLVVVNKEYLCHAAGLGNLF
                     ELASFNTIENQKPDAIYIYGMGKDFFKDKEDVNGVIYREKDGMYVGIVGDDPSVDYFG
                     YMKKMILTLHNLIVIDEGRLPVHGAFAEIKLRDGKKANIMLMGDSGAGKSETLDALNR
                     LRDEVSEVNILIDDMGSFDIDEDGTVVAYGTETGAFVRLDDLQPGYAYSAMDRSIFMN
                     PNEVNARVIVPYSNYGDIIKPTKVDYFFYANNYDRIEDSNAIHFFNETDRAVEVFSKG
                     ARMAKGTTSEKGLSYSYFANPFGAIQRKEKHNDIAKKYMKHMIENNVRIGELKTQLGI
                     DGFEQQGPMIAAKALLKLIEETEK"
     gene            8338..9045
                     /locus_tag="PLU28_00435"
     CDS             8338..9045
                     /locus_tag="PLU28_00435"
                     /inference="COORDINATES: protein motif:HMM:TIGR02254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HOG33559.1"
                     /translation="MLSNKKYIFFDLDDTLFDFNLSSKFSLELVFKEHILPLDLKEYG
                     FKKFVEFYKKINSDLWTKYRENKVSKEFLEVHRFSDTLEKLEIHEDDGFSSNLNTIYL
                     DILSKQRNTVKNAVGTLEYLYRKYPLGIITNGFDTVQRKKLNSCMLTDYFKHIVTSEK
                     AGALKPSRQIFDYAISLTGLNRSEIVFVGDEFDVDIKGANESGITGIWLNTGNEQTDS
                     HLKYIEIKDLSDLKKFF"
     gene            9097..10188
                     /locus_tag="PLU28_00440"
     CDS             9097..10188
                     /locus_tag="PLU28_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOG33560.1"
                     /translation="MQELLLGLTSITIKQIGMMVIGAVLIYLAVKKDYEPALLLPIGF
                     GAIMVNIPFSSAQEVLNTLFNAGIATELFPLLIFIGVGAMIDFTPLLQNPVMLLFGAA
                     AQFGIFFTMLIAGFFGFSLKEAASIGIIGAADGPTSIYVASKFAKNLLGPISVAAYSY
                     MSLVPIIQPPVIKALTSKKERRIKMPYRPKEVSKTVKIIFPIVITVVSGILVPNSVAL
                     IGFLMFGNLIRECGVVERLSKSAQNELANIVTILLGITIGSTMRAEEFLHPTTLIIIL
                     MGLVAFVFDTAGGVLFAKFLNLFLKNKINPMVGACGISAFPMSARVINKMGLKEDPNN
                     FLLMHAVSANVAGQIGSVIAGGLILALVK"
     gene            10201..10350
                     /locus_tag="PLU28_00445"
     CDS             10201..10350
                     /locus_tag="PLU28_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG33561.1"
                     /translation="MLSSSSIQVILASLNIMWEGMLGILVFMLLIYLVIILLGKIFPT
                     QKKSQ"
     gene            complement(10369..11169)
                     /locus_tag="PLU28_00450"
     CDS             complement(10369..11169)
                     /locus_tag="PLU28_00450"
                     /inference="COORDINATES: protein motif:HMM:NF013408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HOG33562.1"
                     /translation="MKISEPLVSKPLVYEKVWGDENLNRIFSSQSKQKIGEAWLFSFY
                     PGMCNELYGLSTFRVYGKSDEVLRDEISSFPLLLKLISCSEWLSVQVHPNDTIAMELE
                     NYERGKTECWYFLKDGGQAMIGKDNSTVAKAVRNENWEDVLEKKPFNFQDILFLPAGT
                     VHTMGPGSMLLEIQQSSDITYRLYDWGRDRPVHKEKGLKVLNSIRAGYVISRNSQAFE
                     SPFFRFRKIKDQICEGFGVYVDLKSYKTIVLPRDVSYYFEGEYIEYKK"
     gene            11305..13185
                     /locus_tag="PLU28_00455"
     CDS             11305..13185
                     /locus_tag="PLU28_00455"
                     /inference="COORDINATES: protein motif:HMM:NF028509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HOG33563.1"
                     /translation="MHKKSLLRRFIIIPIVTFFVFAFLITFIFVNFEYDRFNRDSKVY
                     EETYVSCEKQELKSRIEKIIDYIEFQRTYTSQPEEEIKNDIIELISTLRFENDEYIFV
                     FSTSGQVMAHPYISGKTSSGNPINIFDQQEYKVFKSLLEVGNSEQGGFLTYLWKNYPL
                     EQMELKLTYSRFYAPWDWVVSTGVFIEDINAIVDIGKQTMLEQIRKTIIFIVIIFGIV
                     LAGFVFLLYWVTKLIESEIMGFNSKLKTAIYEGKAIDQKTIKYKEISSLVSDMNELIL
                     SFAATQENLRTSEERWQLAIKGNTDGIWDINFKTNTYYFSPRWKEMLGYADDEFENTN
                     AQLRSKIHPDDLPAFDKALSEYLNRLTPFYKVEYRMKCKDGFYKWILSRGQGLWDSSG
                     NIIRIVGSHTDITETKQSEKILMETIKVKDYLLKEIHHRVKNNLQVISSLLNLQAKDV
                     HDPFVIEVFRESQNRIRAMAVLHEKLYGTKDITQIGLKEYISDICRSLIRSYGVYGKV
                     ELDISIRDVSLGMESAVPCGLIVNELVSNSLKYAFRDMEKGLVKITILKDEDSKVVII
                     TEDNGIGLPKDIDIENPSTLGLQLVVSIVENQLHGKIYLCESQGTKFRITFRELNYKE
                     RI"
     gene            13186..14457
                     /locus_tag="PLU28_00460"
     CDS             13186..14457
                     /locus_tag="PLU28_00460"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOG33564.1"
                     /translation="MGDKSKILIVEDEVIVAKDIQISLENLGYEVCGHAVSGQDAISL
                     CETKNPDIVLMDIVLNGPLDGIETSEIIRSLFNLPVIYLTAYSDEATIQKAKITEPFG
                     YIIKPFEERDLKTTIEMALYKYKTDLLLNNKEKWLSTVFNSISEGIITTDMEGNIEFM
                     NRSAEHITGYKIDDACKNHYSKILKATSEETGEEIFDFVSKCIKEGVEVIINDYVNLI
                     TKEGFETPVNITVNVLKDSSEHTIGTVINFVNMSALKNYENELTKYATTDSLTGVLNR
                     KTGLFYLEKQIQLCKRYGNFFSIAYIDVNYLKEINEKFGENCGDEVLKIICSIIKNDI
                     RESDTVCRLGGDEFLILFSMTSIEEARIVWERIEKKLDIYNASKAKPFDISAACGLAQ
                     FEANDDKNLEELINIADEEMYRNKIAFKNKK"
     gene            complement(14494..15213)
                     /gene="nagB"
                     /locus_tag="PLU28_00465"
     CDS             complement(14494..15213)
                     /gene="nagB"
                     /locus_tag="PLU28_00465"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HOG33565.1"
                     /translation="MKILVCKDYEELSRKSAEIIVSQVNQKKDSVLGLATGGTPVGMY
                     QELVKSYREGKVDFGKVITFNLDEYYGVDPKNVNSYHYYMNTNFFDHVNIDRKNINIP
                     NGMSKDIEKECREYDEKIQSLGGIDLQILGVGLNGHIGFNEPARELMSTTHITDLTKE
                     TINANARFFEDISQVPTKAITVGMATILRSKKIVVLINGKNKSKIFEKITGKKITTQI
                     PATLLQLHPDVTIVVDEQAAT"
     gene            complement(15236..16087)
                     /locus_tag="PLU28_00470"
     CDS             complement(15236..16087)
                     /locus_tag="PLU28_00470"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HOG33566.1"
                     /translation="MVANAISFIHSRYDSLTPSEKRLAKYILQDPSVILPMTVSELSS
                     VTGVSEATVIRFVRGVGFNTFQDFKLSLAIENVTTEKNDDQDITLFSKDSAEDILRKV
                     KIGCTRTIESTASIVNPQNLVNAADFIRSAKRIEIFGVGSSAAVGKIFQYKLTRVGYP
                     SFSIEDPHMQSISAATMTTGDLVVGISLSGSTKDTVDAVCVAKEHGANVICITDHIKS
                     PITKYADVVLETYSRENPIKNVAGRSILAQIYLVEVLTGLLYSTDTQYVQKAGKETAK
                     AVVNKLY"
     gene            complement(16296..16664)
                     /locus_tag="PLU28_00475"
     CDS             complement(16296..16664)
                     /locus_tag="PLU28_00475"
                     /inference="COORDINATES: protein motif:HMM:NF019507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1667 domain-containing protein"
                     /protein_id="HOG33567.1"
                     /translation="MRTVEIVCVQCPMGCKLKITMDEENKISDIAGYTCERGKQYAIQ
                     EITDPKRIIPSSIKVVNGELELVSVKTDRPVSKKLIPEIMIIIKNSMVTAPVKVGQVL
                     VKNILGTGADIVATRQVNAK"
     gene            complement(16665..17897)
                     /locus_tag="PLU28_00480"
     CDS             complement(16665..17897)
                     /locus_tag="PLU28_00480"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOG33568.1"
                     /translation="MKSYNTDVAVIGAGAAGLSAALAASREGARVLLIERNEDSGGIL
                     NQCIHNGFGLHYFKTELTGPEYAFRLAQMIEEKSIEVLTQRYLLDIDSQKKQIKVVSE
                     KGIEFINTKSVVYTCGARERPFGSLLVPSSRACGVYTAGVAQRFINLENKMIGKSAII
                     LGSGDIGLIMARRLTLEGVKVQAVIERMPYPGGLVRNINQCLKDFDIPLYLSHTVTQV
                     RGDARINEVVVCQVDEKYCAVPQTEKIFSVDTLILSVGLIPETSKLYGKVNLDPSTKG
                     VIIDSNGQSSDPFIFAAGNCVIVYDLVDYVSAEGENAGRNAALYAAGKEQDEKITIEK
                     GNNIGIINPSFYVRNTALDIYARVRKPVEKASFVVRDSDEVIFSVKNKSYVPSEMIHI
                     KIDKEKLSRVKKLTVEVI"
     gene            complement(17908..19344)
                     /locus_tag="PLU28_00485"
     CDS             complement(17908..19344)
                     /locus_tag="PLU28_00485"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOG33569.1"
                     /translation="MNIIIIGAGVVGSLIARELCRYNVSVTLIEKNIDVGQEVTKANS
                     AIIHAGYDDEPSSVRAKFCVKGNAMYTNLSQELDFEVKRIGSLVAAFNEEEVNTITEL
                     YNRGINNNVPNLKLISPEQALEIEPNLNPSVKAALYAPTAGITEPWTVAIAAVENAVA
                     NGLNLVLEEKVTGIETQDKKVKKVITDKGSYTADIVINAAGLYADDIAAMVCSDYRPL
                     HPRRGEYILLSKVTSSDLVKTIVFPAPGKMGKGVLVLPTIDGGILLGPTSQDLDQRYK
                     EDKSTTQDGIDFIIEKTRQLIPNIDIRNAIKTFAGLRPESPQKDFWIEESQEIDGFIN
                     ASAMRSPGLTAAPAVAMYISQQISQKYSLKPNLLFNPQRKRIVNYINELGDEEYDALI
                     KQDPLAGKIICSCNKVSEREIIQAIKRGARSIDGVKFRTRAMFGDCQGSFCTFKIMQI
                     LSRELGIDYKDIRYNKPDSRPIVGNTRD"
     gene            complement(19358..20851)
                     /gene="glpK"
                     /locus_tag="PLU28_00490"
     CDS             complement(19358..20851)
                     /gene="glpK"
                     /locus_tag="PLU28_00490"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HOG33570.1"
                     /translation="MKKYICAIDQGTTSTRFMIFNKSGEVVAFDQMEHRQIYPYSGWV
                     EHDPLEIIEKTLKVIIGGLAKSGIEISEISGIGITNQRETTVLWDKTTGKPYYNAIVW
                     QDVRTDDMCRELNRTCGERIREKTGLPVNTYFSASKIKWIIDNIPQVKKDLDKGKVLF
                     GNIDSWLIWKLTGGPGRGVHVTDVSNASRTQLMNIKTLEWDKELLDIFGIPSEILPQI
                     RPSSDFYGCTTVLLEDIPICGDLGDQQAAMFGQNCFESGEVKNTYGTGCFMLMNTGNT
                     PIISNNGLLTTIGYKIGKNDPVYCLEGSIAIAGALVQWLRDNLGIIESSSDIQELAGQ
                     VEDNGGVYFVPAFSGLFAPYWQSKARGTIVGLTRFVNKYHIARAALEATAFQTKDVIN
                     AMEKDSKVPLKSLKVDGGMVANDLLMQFQADIMNVPVIKPKVTETTALGAAYAAGLAS
                     GFYESVNELKANWKKGKEYLPSMDSLIREKLYKNWKKAIGKSMDWVD"
     gene            complement(20874..21647)
                     /locus_tag="PLU28_00495"
     CDS             complement(20874..21647)
                     /locus_tag="PLU28_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOG33571.1"
                     /translation="MKYDDFPKQTLLASPIALPSFYKGGDTAVLLIHGYTGIPHEFIY
                     VAERLNKEGYTVYIPRLPGHGTSPEDFLQTNRNDWIRKVADTYMDLKSSHKEVYVAGL
                     SMGGLLTILLAAKYDIPKIVLAAPALRTKNRKIYYTPFIKLFSRQIMRDENYTNPDKE
                     MYKIGKVYWNRDYPPMAAELLKLQKMAEKELPNVKSSALTIVSKSDGTVPLEVKDIIE
                     EKIGSGTKKTVVLEKSGHVLINDVEREKVADHIIEWFKK"
     gene            complement(21661..22602)
                     /locus_tag="PLU28_00500"
     CDS             complement(21661..22602)
                     /locus_tag="PLU28_00500"
                     /inference="COORDINATES: protein motif:HMM:NF014798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HOG33572.1"
                     /translation="MFENKEMRQVYSETLCELAQNDSRVVVLEADLMKSSGTQVFKDK
                     FPQRFIDVGVAEQSMISVAAGLSNMGKIPFASSFTAFATRRPFDQITISVAYAGLNVK
                     IVAMDPGVTAELNGGTHMSFEDVALMRSIPGAVIVEPVDSIQLYQFMKQAYEMPGLVY
                     MRVYRKTKELFFNQDDKFTIGKGHLVKDGKDVTIVASGIMLKQALNAAQTLDKQGISA
                     RVVNIHTVKPIDKDIIIESAKKTGAVVVAENHNIINGLCSAVSEVLSENYPVIMSRIG
                     VRDHFGEVGKVDFLMKKYKMTAEDIVESAKEIITKKR"
     gene            complement(22619..23440)
                     /locus_tag="PLU28_00505"
     CDS             complement(22619..23440)
                     /locus_tag="PLU28_00505"
                     /inference="COORDINATES: protein motif:HMM:NF012670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOG33573.1"
                     /translation="MTELEVVGLKEKCKEIRKIILEMIGTLGVGHIGGSLSIVETLVV
                     LYYKEMKVDPKNPHWEERDRFVLSKGHAGPALYAVLADKGFFPYEWIYTLNKPGTNLP
                     SHCDRLKTPGIDMTAGSLGQGFPAAVGMAMAAKLDNMQSRIYAIVGDGESQEGSVWEA
                     LMLAGNKKLDNLTAFIDYNKMQIDDYVENINSLEPIADKYRAFNWNVLTVDGHDVRAI
                     YEAVQSAKQTKGKPSMIILDTVKGKGFKLGEGKVSSHNMNITEEQWKQAVEELYK"
     gene            complement(23565..25763)
                     /locus_tag="PLU28_00510"
     CDS             complement(23565..25763)
                     /locus_tag="PLU28_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HOG33574.1"
                     /translation="MFKKADILNFTAVYRIGNPFKTGATVLDDGGFDFKDQQSIQHFE
                     LIQEKESIILKYKMNDDERVFGLGENLGGLNKRGKTYRLYANDDPNHTPEKESLYSSH
                     PFVIIDGKDKSFGFFIDFPGEMLFDIGFSSYNILEIKVSGRDLDLYIFDTSDKLDIIK
                     QYLMLTGKPYVPPRWAFGFHQCRWSYPDSATIASIADKFRTQGIPCDAIYMDIDYMKD
                     YKVFTVDEEKFPQFGSFVKEMKGKGFRLVPIIDPGVKMQKGYDKFEEGMANNYFCLEK
                     DGKPFVGAVWPGLTCFPDFLRSDVRKWWGESYKALTEQGIEGFWNDMNEPAIFYSQNS
                     MKKILDDVKAFFSGQKIEDIGWASASLRDRMNKMSNSRDDYKSFYQKTDDGKVVCHDD
                     VHNLYGFNMCRAAADGFEDIMPGKRYFLLTRSSYSGAHRFTATWMGDNMSWWEHMLVH
                     IRMLQSLNMSGFFYTGADVGGFGCNSDSQLLIRWTQLGAFTPLFRNHSALGTRKQEPF
                     SFDARSMEYMKDTIRLRYAFLSYAYSEFIESVENLRPFITSISMNFEGERVKDIEDQY
                     MYGRSLMVAPVYTPNAAGRYVHLPQCRWLGWTAKKWEERNLQVYEPGDYYIDSPIDRI
                     PLFIKENSMIVLNEPMEYTDQKDITTLYVVAFISKKASFTYILDDGISKDYEDGVSSM
                     LNINISKQDESYEITIDLDECDEVAIKVQRVFFEIYDEYNELYKEEIEIK"
     gene            complement(25860..>26426)
                     /locus_tag="PLU28_00515"
     CDS             complement(25860..>26426)
                     /locus_tag="PLU28_00515"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG33575.1"
                     /translation="SPSAVLDIYDLSLKLGEKEILKNFSLSVKKGERIAITGRNGCGK
                     TSLFNCITGEIDNYSGEIFFPKYIRIMRSYQVPRWQQGYLKDNILRTGVDETVFRNVM
                     GILGVSGDIFLKPLQEFSEGERKKIDLCRTLIEPSSLLLWDEPLNYLDIASRELLEQT
                     ILKFEPTILFIEHDRYFIENVAQRVIAF"
BASE COUNT         7801 a         5481 c         4728 g         8416 t
ORIGIN      
        1 ttcttttttt catggtttta tcagtatcgc ttatgggact ttttgaactg attgtctcaa
       61 accggtcttt tccagtcttg gcatatggtt catacagaaa catattccat tattttgtgc
      121 tgaatatgac atccaaaact atcatagaag tactattctt tacagtaata acagtatttt
      181 ttctttttct ttcagtctcc atatttaaac aacgcgagtt caatgcatga cttctcctat
      241 cgaaacaagt cgctgaaaaa aacgggctgc cccaaaaaaa aaggcagctc cgttttatta
      301 tctattcctc actcatcata cgtaaaaata cctctagtat ctccgggtca aactgcttac
      361 cggaattctc tcccatctct tttatagcct gagaagtcga aagacctttt ctataagatc
      421 tgtcagttct cattgcatcc cacgaatcgg caatggcaat tattctcgaa ataagaggta
      481 ttccctcttc tttaagacca tcgggatagc cgtttccatc ccatctttca tgatggtgct
      541 ttatatacac ggcaacatct ttaagctgat cagacttatt cagaacctca tatccataaa
      601 caggatgctt ctttattgct tcatactcct gaattgttag tctccctggc ttattgacaa
      661 tatcaggact taccagtatc ttaccgatat catgaagcaa cccgctccaa taaagatctt
      721 tcatctgttt ctgagtaaga ccaagcctgg caccaatctt gagcgagcaa ttcgccacag
      781 agtctgaatg acctttggtg tagttgtcgt atatttcaag gatacttacc atagatatta
      841 ttatctgcct ttggaactca ttctgcatac tagagaatct ctgcatagtc aggaaagccg
      901 atgcaaagct ggcaagggtc tctaaaacct ttgatgcatc acgtgaaaac tttatatttt
      961 tatgtctgct tatttcaagg cataatccgc cagcatcgtt gctgcctacc gcgagtttta
     1021 tcaccattgc ctgaccgcat ggttttttta ctctcagata agtctgcaag gtattttgag
     1081 atactgtttt ttcatttatg aactctttgt tttccagaag cagtatcttt tgaaagtcag
     1141 gaatattctc tttcttaagt gaaaggtcat tcagatcatc aacattgtgt cccaaagcat
     1201 ctataaaaac ccactcaccg tcttctataa ggtaaacata cccgtaatca gcctggggaa
     1261 caagctcttt actgagagaa agaacttttt tcagaaactc cctgtcactt acgataacag
     1321 cattgctaag atcaataccc agatttatga tcttttctaa cttctcgttt gtgctttgcg
     1381 tttcagaata agaattttca agctcctcat ttattgctct gagctcttgt atgtttgcgg
     1441 acagctcgtt cgccatttca ttataagtct gaacaagcat atttatttcc ttaacctgac
     1501 tgctttcgat tcctggctgt acaacataga ctttagtttt tgcaaactcc tgcatattcg
     1561 acgaaagaat cgaaaacggc tttatgactt tgttaacagt tttgttcatg gcaaaagtta
     1621 taatgatgct gacagcgatg actgcaccga aaaagaagag tgtaagaaga attctgaggt
     1681 tgtccagaaa agaaaagtcc aactctattt ttataaagta cttttcttcc tggccattat
     1741 cggtcattat atggcataca gagtagtaac tgcgtattcc gccggccata gaaatttcct
     1801 gtgggaaaga tgtatccaaa acttctttga aaacgaactt ttcgttatct ttaaggcctt
     1861 tccatgaata aagcggtaaa agattgggat cgtagatgct tatttctttt atataaaaag
     1921 aactttcttt gactcttgtt atatcgtcaa gaatactttt tttgtgagag ttgcggatag
     1981 ttctatagat attggccaca gactggtcat aaactttttt gtaaacattt ctttcaaaaa
     2041 aaacattaag aaacagccat aaaacaacgg ctgtaaaaaa ggttatgatc agaaaaatcc
     2101 aaaacatcct tttgctgatc atttttttta acgactgctc tttactcatg gtacccccga
     2161 taaagatttc atagaataat gtaaccaagt taaaacagta gtgttcagat aaattatatc
     2221 actggtgcgc ttaatttaaa agttaataac taaatttgtt acatttgact ttacaacttt
     2281 tctttgtatc tttttaaatc gtattgttgc attattttaa aatgtgtatg acttttattt
     2341 gacaaaacct atgtaattat gctataatgg tttatcctat gtactaattt ttaagttact
     2401 tttatgtaac tttttgttaa tattcgattt tttgtgaaag gagatggtga agttgcgtat
     2461 tgccattatc ggagcaggaa actcaggaat ttcaatggcc gcacatctcg cattatcagg
     2521 agaatgtgta aatctttgga acaggtcggc acaaactata aaggatcttg ttccaaccaa
     2581 aagcataaca gttcagggaa aagtaaatgg caaggcttat ctgaacatag ttacagattc
     2641 aatagagctt gccgtaaaag atgccgaaat gatttttgtc acaacaccgg caacttctca
     2701 tgcacagata gcccagagac tggcacctta tgtggataaa aacgtgccta taatacttac
     2761 tcccggaaga accttcgggg caattgagtt taagcatatt cttctacaga acgggtacag
     2821 cggaaacgtt aatgtatgtg agacacagac tataatctac acatgcagga agacagactc
     2881 atgcaatgct gtaattcttg atcttaagaa aaatgttctt atatcgtgtc tggacccaag
     2941 caagatagag agtatcgtat cgagtatgcc tcaatgtctt aaaaaaattt ataagccgac
     3001 caaatccatg gtggaaacct ccataggaaa tgtcggtatg atcctgcact gtacgccaac
     3061 acttctaaac agcggctgga ttgaaagtcc aagtcattcc ttcaaatact attaccatgg
     3121 aataagtcca agcatctctt cgtttctgga acgccttgat gaagagagaa tccatatttc
     3181 tgaagttctt ggtaattctg tcgaatccac ttatcagtgg atgaagagaa gctacggagt
     3241 ggaaggcgaa accctttttc agtgtataca aaacaatgaa gcatataaag aaatagatgc
     3301 tccagatact cttgaacacc gttacatcct tgaggatgtg ccatgtggcc ttgttcctct
     3361 ggaatcgata ggtgcaaaac ttggggtaaa aacaccgtat gtaagtctca taatagatct
     3421 tgctaactcc atgctcaaaa aagatttccg tagtgaaggc agaaccttag aaaagcttgg
     3481 tatgggaagt atgctgcctg agcagatcat aaccatcctg aatcaccagg agctgtatag
     3541 tttatgaacg gaaaaagagt gtttggtttc gtaaaaccag ccatagatgc acattcattg
     3601 ggaatgtttt cactggcgga aataataaaa gagtgcggat atacggtggt actggctgac
     3661 actgaaataa attcagcact agaaaatctt tcttccaaaa agaatctttc agtttttttg
     3721 agatggctta aaaacaacag aataacagat ataggtttta gctacagact tgatccaaat
     3781 gacggtttca gcttgttttc aaacatgatg tttttcctta ttaagaacag aatgctcaga
     3841 acaggcggaa agagtattca gaatatttac tacgcaggcc tggcagaagg ctgtcgaaaa
     3901 gtcgaacagg agtttggaag gtatgcaaaa gtttttatag gaggggaaag tataagtgaa
     3961 actctaaata ttctctcagt tgacagctcc tccattcctc ctcagatgcg agagtcctgc
     4021 cgttatgaca acttcctttt tgatttttca cgcgatatta taaaaaaagg tgagtatact
     4081 tcctttaaac ctgttgaaag aaacggctat ggtggatatg aaggttatgg cacaaagaaa
     4141 gatactctct taaaaagact tgagcattca cagaaaaata atcttcctcc tataataagg
     4201 gctcatgcgg gaccttatct ccaagaaagg gagaaagcag taaaattgtt ttacgaatgg
     4261 tgcgaagaac ttggaaaagc aggtatgctg gacgttcttt ctataggctc ttctcaattg
     4321 acccagtctc acttcggaca ggactggcag gatcttccga acggcggagg agttccggta
     4381 aattcgattc aggagtactc taaaatatgg gaaagttcac gtcccatgct ggtaagaacc
     4441 tatgccggaa ctaaagatct tttgaaactt gctatgatgt atgaggatac tatcaacatc
     4501 gcatggcacg cgctttctct atggtggttc agccagatag acggccgagg tggaaacgga
     4561 gtttatgaaa atcttaaaga acatattcag accattcaat atgcagcttt gaccaacaag
     4621 ccctttgaac ctaatgtccc gcaccatttt gcatttaggg gcgcagatga tgtgacttac
     4681 atagtatcgg catatctggc tgccatgact gcaaaaaaga acggcataaa gcatctgatc
     4741 cttcagaata tgctaaatac accaaagtat acatggaata tagccgatct tgcaaaatca
     4801 agagctatgc ttgagcttgt aagaagcctt gaagatgagg attttaaggt cactctccag
     4861 acaagagcag gtcttgatta tttttctcct gatcccgaaa aagcaaaaat acaattggga
     4921 gccgtaactg cccttatgga cgatatacga ccgcaagatc ctcatagccc tggtataata
     4981 cacgtggtca gttacagcga agggtatggt ctggcagatc ctcaagttat aaacgaatct
     5041 ataaaaataa ctcttcatac gctcgaagag tacaggaagc ttaaaaaaac cgtatctgtt
     5101 atgaaagagt tcggtctttc tgaaattgac cagcgaaaag attcattatt gcaatcggca
     5161 aagaaaataa tatccaccat gcaagaaaaa atacccaacc tttactcggc ttcaggtctt
     5221 tatacagcct ttgcttgcgg gtttcttcca gtaccttatc tttaccttca gaaggaagag
     5281 tttgagtatg ccagggcagt aaagactaaa ataatgaacg gttctgtagt aaaaacagat
     5341 gaatatggga acactatgac agatgagcag ttccttgact atgcaaagtc caatatatca
     5401 aaaattcaga ttcagtcttt aatgagatgg ggggataatt tttgaaaata gccgttgtat
     5461 acgataccga tacatatgaa caaaagaaaa acatggtgac ggcagtaaaa aatgccctgt
     5521 taaaaaaata tgatgccact gagcttgcat ttgatgaaaa tttcatgtat gagataaaaa
     5581 aatacgacat ggtttttaat ctttcgtctt caggaggtcg ggaatcaaga cagatgcatg
     5641 ttccagcaat acttgacatt atgggaatac ttcataccgg tccgggatca tatacgcatg
     5701 ctctatgcct gaataaaaca gttacaaaga tcatccttag gcagaacggt attcccactc
     5761 cgttagagat aaaacagcag gatgtttttg ctcaccgttg ggaagaaggt acttgtttta
     5821 tcgttaagcc tgaaagagaa ggcagtgcga aaggtatctc tgataagtct gtggtctatg
     5881 atgcgcaaag catgaaaaag caggtcgagt acattaacca tacttttaaa cagcctgctc
     5941 ttatagaaga gtttattatg ggaagggaac tcagtatagg aattgtgggt aatgatgaaa
     6001 gcatggaagt acttcccatc ctggaaatag atttttccgg tttgccgcaa gagattgagc
     6061 atttttactc tcacagggta aaagaggagt atggtcatct tacttcgtat aaatgcccgg
     6121 cctctttgga taacaaaacc gaaagagtca taaaggatta tgccgctaaa atctttaatg
     6181 tccttgagct taaggattac tgcagaatgg atgtcagatt gaatgaaaaa aacgaacctt
     6241 tttttataga tgtaaattct atgcctatgc ttatgcctga ttactctgat ataataaaaa
     6301 tggcaaaagc cgcaagtttg gagtatgatg atttaatctt aaaaatagct caatctgcac
     6361 tgtcaagaaa ataaaaatcc ccttaagggg atttttattt ttctgtctct tcaatcagtt
     6421 ttaaaagagc ctttgccgct atcataggcc cctgctgttc aaatccgtct attccaagct
     6481 gggtcttgag ttctccgatt ctgacattat tctctatcat gtgtttcatg tactttttag
     6541 ctatatcatt gtgcttttct ttcctctgta tagcaccaaa agggttggca aaataactat
     6601 agcttaatcc tttttctgat gtagtacctt ttgccatcct tgctcctttt gaaaaaactt
     6661 caaccgcccg gtcagtttca ttaaaaaaat gtatagcatt tgaatcttca atccggtcat
     6721 agttgttggc ataaaaaaag taatcaactt tagtgggttt tattatgtcc ccataatttg
     6781 aatatggaac tatgactctt gcatttacct cgttaggatt catgaatatg cttctgtcca
     6841 ttgctgagta agcgtatccg ggctgaagat catccagcct aacaaaagca cctgtttctg
     6901 tgccgtaagc tacaactgtt ccatcctcat ctatatcaaa agaacccata tcgtctataa
     6961 gaatatttac ttctgaaact tcatccctta atctgttaag tgcatccaaa gtctccgact
     7021 ttccggctcc actgtctccc ataagcatta tattggcttt cttcccatct ctgagcttta
     7081 tctcggcaaa agcaccgtga acgggcagtc ttccttcgtc tattactata aggttatgca
     7141 gggtaagaat cattttcttc atatatccga agtaatcaac cgaaggatca tctcctacaa
     7201 tccctacgta cattccgtcc ttttctctgt aaataactcc gtttacatcc tctttatcct
     7261 taaaaaagtc tttccccatt ccatatatgt aaatagcgtc aggcttttga ttttctatgg
     7321 tattaaagct tgcaagttca aaaaggtttc ccagaccggc tgcatggcat agatactcct
     7381 tatttaccac tacaagaaag aactttgaag ccacatgtat gggaaaaaca aaccagtctt
     7441 ctgtattttt caatcctatg ctctctaaaa tcggctggtc atatacttta aaaagccctt
     7501 ttctggtgtt agatctggta taaaatataa caggcggttc aaaaacaata ctccagataa
     7561 agttcatatc gtaaagccat ccggcatttt taatttttga attttccttt atttcaggtt
     7621 tgtctatcat aaaacctacc tgtgcgctgc tgggtacctg tctaagaatc ttaagcctgt
     7681 tatcggatat atttataagt atctgtcggt acatactctg cactatattt ttaagatcgg
     7741 agttggtgtt gaccagaagc atctgcttga aaatcctgtc aaagctttca gaagtatact
     7801 tgtctgtttt gtacataaat ctgtgtttgt tcctccaaag attaaaaaga ccttctataa
     7861 actttaccaa ttggtctttt ctgtttccca tcttgggaaa gttatagtaa ggagaaccat
     7921 ctattttttc aataggattt atggtcaatg ccaataaaag ctggattata gcgcttatat
     7981 cgtatttttt aactggatcc acttcatcgg tattttttaa aaaaagttca aagaaagagt
     8041 gaagcgagtt ctttctttcc tgagaatcag aaataagttc actcagtatc tgctcaaaaa
     8101 catttgaatt tattatctga gcaatagcac tgtatgagac ctcgccgttt attatgacac
     8161 ttcgcatagt acacctccgc attcaaaaaa tttataaaat acactattat aattgtactc
     8221 tattatttta ttaaaaaaac aaaaccttgt aaaattaata actaactttt ccattccgtt
     8281 gtcaatgaaa aaaattagtg tataatataa gtagatttta gacggaggta cttaacgatg
     8341 ctctctaata aaaaatatat tttttttgat ttggacgaca ccttatttga ttttaatctg
     8401 agctcaaaat tttcgttgga acttgttttt aaagaacata tacttcctct tgacctgaaa
     8461 gaatacggtt ttaaaaagtt tgttgagttt tataaaaaga taaattccga cttgtggaca
     8521 aagtaccggg aaaataaagt ttccaaagag tttcttgagg ttcacagatt cagcgataca
     8581 cttgaaaaac ttgagattca tgaagatgac ggcttttctt caaacctcaa tactatttac
     8641 cttgatattc tttcaaaaca gcgtaataca gttaaaaatg ctgtcggtac acttgagtac
     8701 ctttacagga aatatccttt aggtataata accaatggat tcgatacagt tcagaggaaa
     8761 aaacttaaca gctgtatgct tactgattat tttaagcata tcgtcacatc cgaaaaagcc
     8821 ggagctctta aaccgagcag acagatcttt gactatgcca taagtcttac cggcttgaac
     8881 aggtcagaga tagtttttgt cggtgatgag tttgacgttg acataaaagg ggcaaatgaa
     8941 agcgggatta ccggtatctg gcttaatacg ggaaatgaac agacagactc tcatttgaaa
     9001 tacattgaga ttaaagatct ttcggattta aaaaagtttt tctgattatt aataagctca
     9061 aagcttctgc tcgagtaaat atattggagg gatggcatgc aagaattact tttgggactt
     9121 acttccataa ctatcaaaca gattggtatg atggttatag gagcagtttt gatatacctt
     9181 gccgtgaaga aggattatga acctgcttta ctgcttccga tcggtttcgg ggcaataatg
     9241 gtcaatatac cgttttcttc ggcacaggag gttctgaaca ctctttttaa cgcaggaata
     9301 gcaacagaac tttttccgtt gcttattttc ataggagtcg gagctatgat agactttact
     9361 ccgctgctgc aaaatcctgt gatgctgctt ttcggagctg cagcacaatt cggaatattt
     9421 ttcaccatgc ttatagccgg atttttcgga ttttcactta aagaagctgc ttctatcgga
     9481 atcatcggtg cagcagacgg tcctacttcc atctatgtgg catccaagtt tgctaaaaat
     9541 cttttgggac cgatttcggt agccgcctac tcttatatgt cgttggttcc gataattcag
     9601 ccacctgtca ttaaggcatt gacctcaaaa aaagaaaggc gtattaagat gccttataga
     9661 cccaaggaag tttctaaaac agttaaaata atctttccga ttgtaatcac tgttgtatcc
     9721 ggtatacttg taccaaactc ggtggctctc ataggatttc taatgttcgg aaatcttata
     9781 agagagtgcg gagtagttga aagactttca aaatccgcac agaatgaact tgcaaatatt
     9841 gtcaccatac ttcttggaat aaccataggt tctacaatgc gtgccgaaga atttcttcat
     9901 cctacaacac tcataataat cttaatgggg cttgtagcat ttgtatttga tacggctgga
     9961 ggcgttcttt ttgctaaatt cctgaacttg tttctaaaga acaagatcaa tcctatggtt
    10021 ggggcatgcg gtatttcggc atttcccatg tcagcaaggg ttataaacaa aatgggacta
    10081 aaagaagatc ctaacaactt tcttctcatg cacgctgttt cagctaacgt agccggacag
    10141 atcggttctg taatagccgg cggacttatt ctggcactgg taaaataagg aggaattttt
    10201 atgctgtcat catcatctat ccaggtcatt ctggcctcgc tgaatataat gtgggaggga
    10261 atgctgggaa ttcttgtgtt tatgctcctc atatatttgg taataattct tttgggaaaa
    10321 atttttccaa ctcaaaaaaa atcacaatga aaccggccta aggccggttt attttttgta
    10381 ctcgatatac tctccttcaa aatagtaact tacatctctg ggaagaacta ttgttttata
    10441 gctttttaga tcaacataaa ctccaaatcc ttcacagatc tgatccttta ttttacggaa
    10501 tctgaaaaag ggactttcaa aagcctgaga gttacggctt atcacataac ctgctctgat
    10561 actgttaagc accttcaagc ctttttcttt gtgtactggc ctgtccctgc cccaatcata
    10621 aagcctatag gtaatatctg agctttgctg tatttctaaa agcatgctgc ccggtcccat
    10681 agtatgaacc gtacctgcgg gaagaaacaa tatatcctga aaattgaaag gttttttctc
    10741 tagaacatct tcccaatttt catttctgac agcttttgca actgtagagt tatcttttcc
    10801 gatcatcgcc tggcccccat ccttcagaaa gtaccagcac tcggtctttc ccctttcata
    10861 attttcaagc tccatagcaa tagtatcgtt gggatgtacc tgtacagaaa gccattcaga
    10921 acaagaaata agtttaagca aaagaggaaa gctggatatt tcatccctga gtacctcatc
    10981 agactttccg tatactctaa aggtagatag tccgtataat tcattgcaca tacccggata
    11041 aaaagaaaaa agccaagcct cacctatctt ttgtttgctt tgggacgaaa aaatacggtt
    11101 aagattttca tccccccaga ctttttcata gacaagtggt ttagaaacaa gcggttcgct
    11161 gattttcaac gtgttcacct ctttaaatta ttttcactgc ttttattata ccactataaa
    11221 agaatcataa tttttattgt ataattaact tatatatact aagaatcaat tttattttat
    11281 ctataatata tcggcggtgt cctcatgcac aaaaagagtc tgctcagacg tttcataata
    11341 ataccaatcg ttactttctt tgtttttgct ttcttaatca ccttcatatt tgtgaacttt
    11401 gagtacgacc gctttaacag agacagcaag gtctatgaag agacctatgt atcctgtgag
    11461 aaacaggagt taaagtcaag gatagaaaag atcatagact acattgaatt tcagcgtacc
    11521 tacacctccc agccggagga agaaattaaa aacgatataa tagaactcat cagtacatta
    11581 cggttcgaaa acgatgagta tatttttgtt ttctcaacct caggtcaagt catggctcat
    11641 ccatacatct ccggaaaaac cagcagcggt aatcccataa atatatttga ccagcaggag
    11701 tataaagtgt ttaaaagtct tctggaggta ggaaactctg aacaaggtgg atttttaact
    11761 tatttatgga agaactatcc tttggaacag atggagctaa aacttaccta ttcacgattt
    11821 tacgctccat gggactgggt tgtatccaca ggggttttta tagaggatat caatgctatt
    11881 gttgacatag gaaaacaaac gatgttggaa cagatcagaa aaactataat ctttatagta
    11941 ataatatttg gtatcgtgct ggcaggattt gtttttttgc tttactgggt aaccaagctt
    12001 atagaaagtg agatcatggg ttttaattca aaactcaaaa cggctatata tgaaggaaaa
    12061 gccatcgatc aaaaaaccat aaagtataag gagataagct cactggtcag cgatatgaat
    12121 gaacttattc tctcttttgc tgcaacccaa gaaaatctac gcacaagcga agagcgatgg
    12181 cagctggcta taaaaggcaa cactgacggt atatgggaca taaactttaa aaccaacacc
    12241 tattacttct caccacgatg gaaagagatg ttaggatatg ccgatgatga gttcgaaaat
    12301 accaatgcgc agttgagatc caaaatacat cccgatgacc tgccggcatt tgacaaagct
    12361 ctttctgagt atcttaacag gcttactccg ttttacaaag tcgaatacag gatgaaatgc
    12421 aaagacggat tttacaaatg gatcctgtca cgtggccaag ggctttggga cagttctggt
    12481 aatataataa gaatagtggg ttcacatacc gacataacag aaaccaaaca gtctgaaaaa
    12541 attcttatgg aaaccataaa ggtcaaagac tatcttttaa aagaaattca ccatagagta
    12601 aaaaataatc tccaggttat ctcaagtctt ttgaaccttc aggcaaagga tgttcatgat
    12661 ccttttgtaa tagaagtttt ccgtgaaagc caaaacagga taagagctat ggcagttctt
    12721 catgagaagc tttacggaac caaggacata acccagattg gtcttaaaga gtacataagt
    12781 gacatatgcc gcagtcttat acggtcgtat ggagtgtatg gaaaggtcga gcttgacatt
    12841 tcaataaggg acgtttcttt ggggatggaa agtgctgtac catgcggact tatcgtaaac
    12901 gaactggttt ccaactctct gaaatatgct tttagggata tggaaaaagg actcgtgaaa
    12961 attacaattc tcaaagacga agattcaaag gtggttataa ttacagaaga caacggaatc
    13021 ggtcttccaa aagatattga tatcgagaac ccttctacgt tgggtcttca gctggttgta
    13081 agtatagttg aaaatcagct ccacggaaag atatatcttt gtgaatctca gggaaccaaa
    13141 ttcagaataa ctttcaggga gctgaattat aaggagagga tttagatggg ggacaaaagt
    13201 aagatactaa tagtagaaga cgaggtaatt gtagccaaag atatacagat ctctcttgaa
    13261 aatcttggat atgaagtctg tggtcatgca gtttccggtc aggacgccat aagtctttgc
    13321 gagactaaaa atcctgatat agtcctcatg gacattgttt taaacggtcc gcttgacgga
    13381 attgaaacct ctgagataat cagatcactt ttcaatctgc cggtaatcta tttaactgcc
    13441 tattccgacg aagccactat acaaaaagcc aaaattacgg aaccttttgg atatataata
    13501 aagccctttg aggaacgtga tttaaaaaca actattgaaa tggctcttta caagtataaa
    13561 acagatctgc ttctcaataa caaagaaaag tggcttagca ctgtatttaa cagtatttcg
    13621 gaaggcataa tcaccacaga catggaagga aatattgaat tcatgaacag aagtgccgaa
    13681 catattacag gttataagat agatgatgcc tgtaaaaacc attacagcaa aatacttaaa
    13741 gccactagtg aagaaacagg cgaagaaatt tttgattttg tttcaaagtg cataaaagaa
    13801 ggtgttgaag taataataaa tgattatgta aaccttataa ccaaagaagg gtttgaaact
    13861 ccggtaaaca taacagttaa tgttctcaag gactcttcag agcatactat aggcactgtt
    13921 attaactttg taaatatgtc cgctctaaaa aactatgaaa acgaacttac aaagtatgct
    13981 acaactgata gtcttacagg agttttaaac agaaaaacag gactgttcta ccttgaaaaa
    14041 cagatacagc tttgcaaaag atatggaaat tttttctcca tagcttatat tgacgtaaac
    14101 tatctcaagg agataaatga gaagttcgga gaaaactgcg gtgatgaagt actgaaaata
    14161 atatgttcta taattaaaaa cgatatacgt gaatcagata ctgtttgccg attaggcgga
    14221 gatgagttcc taattctttt ttcgatgaca agcatagagg aagccaggat cgtctgggaa
    14281 agaattgaaa aaaagcttga catatataat gcttcaaaag ccaaaccttt tgatatttca
    14341 gcagcatgcg gacttgctca gtttgaggca aacgacgata aaaatctcga agagcttatt
    14401 aatattgctg atgaagaaat gtatagaaac aagattgctt ttaaaaataa aaagtagaat
    14461 aaaaacataa aacgaaagcc ttaggctttc gttttatgtt gctgcttgtt catctacaac
    14521 tatagtaaca tcagggtgta actgtaaaag ggttgctggt atttgggttg ttattttttt
    14581 accggttatc ttctcaaaaa tctttgattt attcttaccg tttatcagta caactatctt
    14641 tttcgatcta agtatagttg ccattcccac agttattgcc ttcgtaggaa cctgtgaaat
    14701 atcttcaaag aaccttgcat ttgcgttaat cgtttctttg gtaagatcag taatatgggt
    14761 tgtggacata agctctcttg cgggttcgtt aaaaccgata tgtccgttaa gacctactcc
    14821 gagaatctga agatctatac cgcccaaact ctgaatcttt tcatcatact cacgacattc
    14881 tttttcgata tctttactca taccgtttgg aatatttatg tttttcctgt caatattcac
    14941 atgatcaaaa aaattggtat tcatatagta atgatagctg ttgacgtttt tgggatcaac
    15001 tccgtaatac tcatcaagat taaaggtaat tacctttcca aaatcaacct ttccttcacg
    15061 atagcttttt acgagttcct ggtacattcc cacaggggtt ccgcctgttg ctaaacccag
    15121 tacggaatct tttttttgat tgacctggct cacaataatt tccgcagatt tgcggcttaa
    15181 ctcttcataa tctttacaaa caagaatctt catatttcct ctcctcaaca aataatcaat
    15241 aaagcttgtt tacaacagcc ttcgctgttt ctttgcccgc tttttggaca tactgcgtgt
    15301 ccgttgagta caacaggccc gtaagtactt caacaagata tatctgagca agaattgatc
    15361 ttcctgcaac attttttatg ggattctccc tggagtatgt ttctaaaaca acatcagcat
    15421 acttggtaat gggcgattta atgtggtctg ttatgcatat tacattcgct ccgtgctctt
    15481 ttgcaacaca tacggcatca acggtatcct tggttgaacc actgagactt attcctacta
    15541 caaggtcacc tgttgtcatt gtggctgctg atatagactg catatgcgga tcttctattg
    15601 aaaaactcgg ataaccaacc cttgtaagtt tatactggaa tattttgcct acagcagccg
    15661 atgatccgac accgaatatt tctattctct tggcagacct tataaaatcg gcagcattca
    15721 ccagattctg tggattgact atagatgctg tactttctat ggttcttgta cagcctattt
    15781 ttaccttacg caaaatatct tctgcactgt ctttcgaaaa aagagttata tcctggtcat
    15841 catttttttc tgttgttaca ttctctattg caagagaaag cttgaagtcc tgaaaagtat
    15901 tgaaccctac acctctcaca aatcttatta cagttgcctc gctgactccg gtaacagaac
    15961 tcagctcact caccgtcata ggaagtatga ccgaaggatc ctgaagtata tactttgcaa
    16021 gccttttctc agagggtgta agagaatcat accttgaatg gatgaaggat atggcattgg
    16081 ctaccaactt gcacctctat ggataaaata ttatttcttc atgattcaaa aaaaatgaag
    16141 ctttactaca attactatta tacacaaact tttattttta ttgaaagatc tttaaaggca
    16201 actacaagcc tatactaact tttaattctt tttttggtga aaataatcgt attacggcat
    16261 ccgtgctcta tccgtgttaa gtttttcctg ttagcttact tcgcattaac ctgtcttgtt
    16321 gcaactatat cagcaccggt tcccagaata ttttttacaa gaacctgtcc taccttaaca
    16381 ggagctgtaa ccatactatt ttttatgatt atcataattt caggtatgag ttttttcgaa
    16441 acaggtctgt cggtctttac tgacacaagc tcaagctctc cgttaactac ttttatgctt
    16501 gagggtataa ttctttttgg atcggttatt tcctgtatgg catactgttt tcccctctcg
    16561 catgtgtacc cggcaatatc ggatatctta ttttcttcat ccatagttat tttgagctta
    16621 caacccatag ggcactgaac acatactatc tcaactgtcc gcatctatat gacctccacg
    16681 gtaagttttt ttacccggga aagtttttct ttgtctatct ttatatgtat catctcactg
    16741 ggaacataac ttttattttt tactgaaaag attacctcat cagaatctct taccacaaaa
    16801 gaggcttttt ccacaggctt tcttactctt gcataaatat ccagagctgt gtttctaaca
    16861 taaaatgacg gattaattat tcctatgttg ttgccttttt ctatcgtaat tttttcatcc
    16921 tgttctttcc ctgctgcata taaagccgca tttctaccgg cattttctcc ttctgcagaa
    16981 acataatcta caagatcata tacgattaca cagttgcctg ccgcaaaaat aaaaggatca
    17041 gacgactgtc cgttgctgtc gataataacg cctttcgtag aaggatctaa gtttaccttt
    17101 ccataaagct tagacgtttc cggtataagt cccacagaaa gaataagtgt atctacactg
    17161 aatatttttt cggtttgtgg cacagcacag tacttttcat ccacctggca aacaacaacc
    17221 tcgtttattc tggcatctcc acgtacctgt gtcacagtat gactaagata aagcggtatg
    17281 tcaaaatctt taaggcattg gtttatattt ctcaccaggc cgcctggata aggcattctt
    17341 tctatcacag cctgtacctt aactccttct agagtaagtc ttctggccat tatcaggcca
    17401 atatcccctg agccaagaat aattgcactt tttccaatca ttttgttttc aagatttatg
    17461 aatctttgag ctacacctgc cgtataaacc ccacatgctc ttgaagaagg tacaagaagc
    17521 gacccaaaag gtctttcccg tgctccgcat gtataaacca cgcttttcgt atttataaac
    17581 tctatcccct tttccgaaac aacttttatc tgtttctttt gggaatcaat atcaagcaga
    17641 tatctctgag taaggacttc tatagacttt tcctcaatca tctgagctaa tctaaaagca
    17701 tactccggac ctgtaagctc agttttaaag taatgaagcc caaatccgtt gtgaatacat
    17761 tgattcagta tccctccgct gtcttcgttc ctttcaatca aaagtacccg tgctccttcc
    17821 ctgcttgcgg caagggcagc acttaaacct gccgcacccg ctcctataac ggcaacatca
    17881 gtattgtaag atttcatttt ctcctcctca atcccttgtg ttgcccacaa taggcctgga
    17941 atccggttta ttgtaacgta tatctttgta atcaatccca agctctcttg agagaatctg
    18001 cattatttta aaggtgcaaa aacttccctg acaatcgcca aacattgccc tagtcctaaa
    18061 cttcactcca tcaatgcttc ttgctcccct ttttatggcc tgaataatct cacgttcgct
    18121 caccttatta cacgaacaga ttatttttcc ggcaagagga tcctgtttta tcaaagcatc
    18181 atactcctcg tccccgagct cgtttatgta gttcactatg cgtttgcgct gcggattgaa
    18241 aaggaggttg ggcttaagcg aatatttctg tgatatctgc tgggaaatat acatagctac
    18301 agccggagca gccgtaagac cgggcgaacg catcgcactt gcatttataa acccatctat
    18361 ctcctgactc tcttcaatcc aaaaatcttt ctgaggactt tcaggtctta accctgcaaa
    18421 agtcttaatg gcatttctta tatcaatatt aggaataagt tgccttgttt tttctattat
    18481 aaaatcaatt ccatcctgtg tggtggattt gtcttccttg tacctctggt caagatcctg
    18541 tgaagtcggg ccaagcagaa tacctccatc aatagtagga agcaccagaa ctccttttcc
    18601 catctttcca ggagcaggaa agactattgt ttttacgaga tcagaggatg taaccttact
    18661 taaaagtata tactcacctc ttctgggatg aagcggtctg taatcgctgc ataccatggc
    18721 tgctatatca tcagcataca ggcctgcggc attgataact atatcggcag tataacttcc
    18781 tttatcggta ataacttttt ttactttttt gtcttgagtt tctatacctg taactttttc
    18841 ttccagaaca agattcagcc cgtttgcaac ggcattttca acagcggcaa tggcaactgt
    18901 ccatggctct gttattcctg cggtaggtgc ataaagagct gcttttaccg aaggattaag
    18961 atttggctct atctcaagcg cctgttccgg tgaaattagc ttcagattcg gaacgttgtt
    19021 gtttattccc ctgttgtaaa gttctgttat tgtgtttacc tcttcttcat tgaatgccgc
    19081 aaccagagag ccgattcttt ttacctcaaa atcaagctct tgtgaaaggt tggtatacat
    19141 ggcgtttcct ttaacacaaa actttgcccg tacactggag ggctcgtcgt catatccggc
    19201 atgtattata gccgaatttg ctttagtaac ctcctgccca acatcaatat ttttttcaat
    19261 cagagtaaca gaaacattat atctgcaaag ctcgcgggca ataagtgagc ctactacccc
    19321 ggcccctatg atgataatat tcatttatcc acctctgtca gtctacccag tccatgcttt
    19381 tcccaatggc ctttttccag ttcttataaa gcttttctct aatcagagaa tccatggagg
    19441 gcaggtattc cttacccttt ttccagttgg ctttgagctc attaacactt tcataaaacc
    19501 ccgacgcaag acctgcagca tatgcagccc caagagctgt ggtttctgtt accttgggct
    19561 tgataaccgg aacgttcatt atgtctgcct gaaactgcat gaggagatcg ttggctacca
    19621 ttcctccatc caccttcagt gatttgagag gaacctttga atctttttcc atggcattga
    19681 ttacatcttt agtctgaaaa gctgtagctt caagggcagc acgggcgatg tgatacttat
    19741 ttacaaaacg tgtcagccct actatggtac cgcgtgcctt gctctgccag tatggggcaa
    19801 aaagacctga aaatgccgga acaaagtata ctcctccatt atcttctact tgacctgcaa
    19861 gttcttgtat atcagaggaa ctttcaatta tgccaagatt atctctgagc cattgaacta
    19921 aggcgccggc aatagctatg gaaccttcaa gacagtatac cggatcattt ttacctattt
    19981 tgtaaccaat ggtggtaaga agaccgttat tgcttattat gggagtgttg ccagtattca
    20041 taagcataaa acatcctgtt ccgtaagtat tttttacttc tccgctttca aaacagttct
    20101 gtccaaacat agctgcctgc tgatcaccaa gatctccgca tataggaata tcttcaagca
    20161 gtacagtcgt acagccataa aagtcagagc tgggtcttat ctgcggtaaa atctctgatg
    20221 gtatcccaaa tatatccaaa agctctttat cccactcaag ggtttttatg ttcataagct
    20281 gtgttctact ggcgtttgaa acatcggtta catgaactcc tcgtccagga cctcctgtaa
    20341 gcttccatat cagccaggaa tctatattcc caaaaagaac ctttccttta tcaagatctt
    20401 ttttgacctg cgggatatta tctatgatcc attttatttt gctggcggaa aaatacgtat
    20461 ttacaggcaa tcctgttttt tcacgtattc tctccccaca tgtgcggttt aattcacgac
    20521 acatatcgtc tgttctgaca tcctgccata caatagcgtt ataataaggt ttgccggtag
    20581 ttttatccca cagcacagtt gtttcacgct gattggttat acctattccg gatatctctg
    20641 aaatctctat accagactta gcaagaccgc cgattattac cttaagagtt ttttcaatta
    20701 tttcaagcgg atcatgttcc acccatcctg aataaggata gatctgtcgg tgttccatct
    20761 ggtcaaacgc aacaacttca ccgcttttat taaatatcat aaatcttgtg cttgtagtcc
    20821 cctggtctat agcacatatg tattttttca tagcttcccc ccaagcaaca gctttatttc
    20881 ttaaaccatt ctataatatg atctgcaacc ttttcccttt ctacatcatt aatgagtaca
    20941 tgtccgctct tttcaagaac tactgttttt ttggtaccag atcctatctt ttcctctatg
    21001 atatccttga cttcaagcgg cactgttccg tctgactttg aaacaattgt aagcgccgaa
    21061 gatttgacgt tgggaagctc cttttcggcc attttctgaa gcttgagaag ttcggcggcc
    21121 ataggaggat agtccctgtt ccaataaact tttcctatct tatacatttc cttgtctgga
    21181 tttgtatagt tttcatcacg cattatctgg cgactgaaca gttttataaa aggagtatag
    21241 taaatctttc tgttttttgt tctcagtgcc ggagcagcca gtactatttt cggaatatca
    21301 tactttgccg caagaagtat agtaagcagt ccccccattg aaagacctgc aacatagact
    21361 tctttatgag aactttttaa atccatatat gtgtcagcaa ctttcctgat ccaatcattg
    21421 cggttcgtct ggagaaaatc ttccggtgat gtgccgtgtc ccggaagtct gggaatataa
    21481 actgtatatc cctctttgtt aagtctttcg gccacataga taaactcatg cggaatgcct
    21541 gtataaccgt gtataaggag aactgccgta tccccgcctt tatagaagct tggcagcgct
    21601 atcggagatg caagcagagt ctgcttcgga aagtcatcgt acttcatata atcaccgtct
    21661 ttatcttttt tttgttatta tttcttttgc tgactcaaca atatcttctg ctgtcatttt
    21721 atattttttc atgaggaaat caacttttcc cacttctcca aaatgatctc ttacacctat
    21781 ccggctcatt attacaggat agttctcgct cagcacttcc gaaacagcac tgcacagacc
    21841 gtttattata ttgtggtttt cagctacgac aacagctcct gttttcttgg ccgattctat
    21901 tattatatct ttatcgatag gctttactgt gtgaatattt accaccctgg cgcttatccc
    21961 ctgcttgtca agagtctgtg ccgcattcaa agcttgtttg agcattatac cgctcgccac
    22021 tatcgtaacg tcttttccgt ctttcacaag atgtcctttt cctattgtaa acttatcatc
    22081 ctgattaaaa aacagttcct tggtttttct gtaaactctc atatatacga gtccgggcat
    22141 ctcatatgcc tgtttcataa actgatacag ctgtattgaa tccaccggtt caacaataac
    22201 ggctcctggt atggatctca taagggccac atcctcaaag ctcatatgag taccgccgtt
    22261 aagctctgcc gtaactcccg gatccatagc aactatctta acgttaagcc ctgcataggc
    22321 aacggatata gttatctggt caaacggtct gcgggttgca aaggctgtaa aagaacttgc
    22381 aaaaggtatc tttcccatat tggaaagtcc ggcagcaacg cttatcatac tctgttcagc
    22441 aactccgaca tcaataaatc tttgggggaa cttatctttg aatacctgcg ttccggaaga
    22501 tttcataaga tctgcttcta aaacgactac acggctgtca ttctgagcca gttcacagag
    22561 cgtctccgaa taaacctgtc tcatctcttt attttcaaac atttattatt cctcctgatt
    22621 atttatataa ttcttctaca gcctgtttcc actgttcttc agttatattc atattatgtg
    22681 aggagacctt tccttcacca agcttaaatc cttttccctt gactgtatcc agaattatca
    22741 tactgggttt gccctttgtc tgctttgcac tttgaactgc ttcatatatg gctctaacgt
    22801 catgtccgtc aactgtcagg acattccagt taaatgctct gtatttgtca gcaataggtt
    22861 caagtgagtt tatgttttca acataatcat ctatctgcat cttgttgtaa tctatgaaag
    22921 ccgtaaggtt atcaagtttt ttgtttccgg caagcatgag tgcttcccat actgatcctt
    22981 cctgtgattc gccatctccg actattgcat atattctgga ttgcatattg tcaagcttgg
    23041 ctgccattgc cattcctaca gctgccggaa atccctgacc caaagaacca gcagtcatat
    23101 caataccagg ggtttttagc ctgtcgcaat gactcgggag attggttccg ggtttattca
    23161 gagtgtatat ccattcatag ggaaaaaagc ctttatcagc cagaacagca taaagagcag
    23221 gtccggcatg acctttggaa agaacaaacc tatctctttc ttcccagtgg ggatttttgg
    23281 gatcaacttt catttccttg tagtacagaa caaccaaagt ttctacgatc gaaagagatc
    23341 ctccgatatg tcccaccccg agagttccga tcatttcaag gatgatcttt cttatctcct
    23401 tgcacttttc cttaagaccg accacttcta gctctgtcaa aataatcacc tcgtatgttt
    23461 tttattgctg tacataaaat tataccaccc atactttttg atattgaaat atccttgtaa
    23521 ataaaaagca cctgcaaagt ttatatcctt tgcggtgcgt tcttttattt tatttctatc
    23581 tcttctttat aaagttcatt gtactcgtca taaatttcaa agaatactct ttgcactttt
    23641 atcgctactt catcgcattc atccagatct atggttattt cataactttc atcttgtttt
    23701 gaaatgttta tattgagcat tgaactcaca ccatcttcat aatcctttga gattccatca
    23761 tccagaatat atgtaaagct cgctttcttt gatataaatg cgaccacata aagtgtggtt
    23821 atatctttct ggtctgtata ctccatcggt tcattcagaa ctatcatgga attttctttt
    23881 ataaaaagag gaatcctgtc gatcggagaa tcaatataat aatcacccgg ttcatacacc
    23941 tgcaaatttc tttcctccca tttttttgct gtccagccca accacctgca ttgaggaaga
    24001 tgtacgtatc tgcctgcagc atttggagta taaacaggtg caaccataag agaacgtccg
    24061 tacatatact gatcctcaat atctttgact ctttcgccct caaagttcat actgattgac
    24121 gttataaacg gtctgagatt ctcaacgctt tctataaact ctgaatatgc gtatgaaaga
    24181 aaagcatacc gtaatcttat ggtatctttc atatactcca tagaccttgc atcaaaagag
    24241 aatggttcct gttttcttgt tccaagagcc gaatgatttc tgaaaagagg agtaaatgcg
    24301 cctaactgcg tccaccttat aagaagctgt gaatcactgt tacagccaaa tcctcccaca
    24361 tctgcaccgg tataaaaaaa gccgctcatg ttcaaagact ggagcattct tatgtgaaca
    24421 agcatatgtt cccaccagct catattgtca cccatccatg tggctgtaaa cctgtgtgcg
    24481 ccgctgtaac tgctcctggt aagcagaaag tatctcttac ccggcattat gtcttcaaac
    24541 ccatcagccg cagcacggca catattgaat ccataaagat tgtgaacatc atcatgacat
    24601 acaacctttc catcatcggt tttctggtaa aagcttttat agtcgtccct actgttggac
    24661 attttattca tacgatctct cagcgaggcg cttgcccatc ctatatcctc aatcttctgt
    24721 cctgaaaaga atgcttttac atcatcaaga atcttcttca tagagttctg tgagtagaag
    24781 atggcaggtt cgttcatatc gttccaaaat ccttctatcc cttgttcagt aagggcttta
    24841 taactttcgc cccaccattt tctgacatct gaccttaaaa aatccggaaa acatgttagt
    24901 cccggccata cggctcccac aaaaggttta ccatcttttt caaggcaaaa gtaattatta
    24961 gccatacctt cttcgaactt atcgtagcct ttctgcattt tgactccggg atctattatc
    25021 ggaacaagtc tgaagccttt tcccttcatt tctttaacaa aacttccgaa ctgtggaaat
    25081 ttttcttcat caaccgtaaa aaccttataa tccttcatgt agtctatatc catgtaaatg
    25141 gcatcacaag gaattccctg cgttctaaac ttatcggcta tagaagctat agttgcactg
    25201 tctggatagc tccacctaca ctggtgaaat ccaaaagccc atcttggagg aacatatggc
    25261 ttacccgtga gcataagata ctgttttatg atatctagct tgtcagaagt atcaaagata
    25321 tacagatcca gatctctgcc gcttactttt atttccaaga tattgtatga agaaaatccg
    25381 atatcaaaaa gcatctctcc gggaaagtct ataaaaaagc cgaacgactt atctttgccg
    25441 tcaataataa caaacggatg agaactgtaa agactttctt tttcaggagt atggttggga
    25501 tcatcattgg cataaagcct atatgttttt cctcttttat tgagacctcc cagattttcc
    25561 ccaagtccga aaactctttc atcatcattc attttgtatt tcaatatgat actctctttt
    25621 tcctgtataa gttcaaagtg ctggatactc tgctggtctt taaaatcgaa tcctccgtca
    25681 tcaagcaccg ttgcacctgt tttgaatgga tttcctattc tgtaaaccgc ggtaaaattt
    25741 agtatatccg cttttttaaa caactcttcc acctccggta tttttacgct atataaatta
    25801 tacattattt catattgaaa ttattacttg tactgtaccg tatgcttatt gctttttgat
    25861 caaaaagcaa ttaccctctg tgcaacattt tctatgaagt atctgtcatg ctctataaac
    25921 agtatcgtcg gttcaaactt gagtatagtc tgttcaagaa gctcccggct ggcaatatca
    25981 agatagttca gcggctcatc ccataaaagc agagatgaag gttcgataag tgtgcggcaa
    26041 agatctattt ttttcctttc accttcgcta aactcctgaa gaggctttaa aaaaatatcg
    26101 ccgcttacgc caagtattcc cataacgttt ctaaaaacgg tttcatccac accggttctg
    26161 agaatattat ccttaagata cccctgctgc catctgggaa cctgataaga ccgcattatc
    26221 cgtatatatt tgggaaaaaa tatttctccg gaataattat ctatctctcc ggtgatacag
    26281 ttaaacaaag aagtttttcc gcatccgttt ctgccggtta tggctattct ttcacctttt
    26341 tttacgctga gactgaaatt tttcaatatt tccttttctc ctaacttcag tgacagatcg
    26401 taaatatcca gtacggccga aggaga
//
LOCUS       DAOSHR010000005        25690 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_1509_length_25690_cov_5.546362, whole genome shotgun sequence.
ACCESSION   DAOSHR010000005 DAOSHR010000000
VERSION     DAOSHR010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 25690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25690
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1509_length_25690_cov_5.546362"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1577
                     /locus_tag="PLU28_00520"
     CDS             <1..1577
                     /locus_tag="PLU28_00520"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HOG33576.1"
                     /translation="TPASLDASKQAVKFEFDGSATNTKVKVTVVEEGKEYSDTYELAD
                     NQVSTINAFFNGGTDVGGKTLIINGKQLKAAALISGAATDRVNINSITWPTMVDGTLA
                     GTSGGTARDFSLAGGIKLTDTVKGQDIYYDPKNVKVTVTNAAGAIPGSVTVSLTTKDG
                     VKNFTINGTVDAGVGKNVSYQQFNEAFSDGVKNGNLRITGLSLSNGQDTDELTATAAT
                     GVTFASVKNIIQPPSGGSPKFVDVNNPQNFVVAGYKAPTVAASTLVYDSLGQAYNVYL
                     NFTKINENTWFWKAQMEDGTPLYKLNEDGTRDMTSFADGVIAFDGNGGIMSTNWRLRE
                     DGTIDTNTTDNNNGSLGFWFDPAKPGAAPNPTYNPSSISAAGPVMAQVKFQELTQFAS
                     PHSIAVTEQDGNSEGTLDSFAINEVGQIIGTFTNGKSDALGQVALAVFNNPAGLLELG
                     NSLYAQSSNSGIPQIGTCGVGGRGTLLPGTLEMSNVDLAEEFTNMIIAQRGFQATSRI
                     ITTADQILNELVNIKR"
     gene            1601..1798
                     /locus_tag="PLU28_00525"
     CDS             1601..1798
                     /locus_tag="PLU28_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HOG33577.1"
                     /translation="MISVTRLNNEGFVINGELIEKLEAKPDTTITLLNGHVYIVKEKV
                     EEVIKKVIEYKKSIFIGPQKE"
     gene            1806..2606
                     /locus_tag="PLU28_00530"
     CDS             1806..2606
                     /locus_tag="PLU28_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOG33578.1"
                     /translation="MDISSLLGLALGIIAFVFGVYSSAGYSFSGLSAMIDVPSMIVTI
                     VGSIGSMFISNPKEKAFKFVSYIMMALKEQKIDMLGTLRTLYTFAEKARRDGMISLEE
                     DIGKIDDEFMRDGLRAAVDGTDPEEIRKILEIKMELYEDDQGGNIAVMESWGAYGPAF
                     GMIGTLIGLILLLGQLNDPTAIGPYMSVALITTLYGALIANILATPVADKLKKRTAKM
                     ISMKRMTTEGILSIVQGENPRLMEEKLKAFLSAQDRATYEKGKGEAQA"
     gene            2621..3496
                     /locus_tag="PLU28_00535"
     CDS             2621..3496
                     /locus_tag="PLU28_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HOG33579.1"
                     /translation="MGKDKCNCPPPAPVWLTTFSDLNNLLTTMFIALFSMATISPGKF
                     QQAASSLASAFNGQPMGVLVGGKTISEEPLITSNPGVKENLLKIQMDENFKGMITIEE
                     TDRGTIIHIKDYAFFETGSAQLTKKAKELLYLISTIILEHTSNDIEVYGYTDERAVLS
                     TSVYPSNWHLSNARAASVAYFITGELKDRRLVELVGQIKSGQFDINYFYEPTRFFPIG
                     RGDADILKEMDELKIANDNRRDILSLDFQKGSMTAQELKDKLASIDKEYEAAKEKLRE
                     DYRRVDLLILRQRVR"
     gene            3508..4044
                     /locus_tag="PLU28_00540"
     CDS             3508..4044
                     /locus_tag="PLU28_00540"
                     /inference="COORDINATES: protein motif:HMM:NF015693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HOG33580.1"
                     /translation="MAQENPVPEEGKKKGGNNFLLMIIIVVVVTLVVSAGTSFLIITL
                     LGQNVQKTADATPSAGTTGTTGLVVQAEMIREGARYPVMLKGGKDVAIVDALYYKVGS
                     EECRSSIGTNKLEILDAVRTIFLNKTSSEVTNPTGQELIKKQIKDAVNEITGYTGERE
                     KIGVLSVILIILTISQNQ"
     gene            4057..5124
                     /gene="fliM"
                     /locus_tag="PLU28_00545"
     CDS             4057..5124
                     /gene="fliM"
                     /locus_tag="PLU28_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HOG33581.1"
                     /translation="MVEGDKLSQDEIDKLLKNMTAPEGESEGSEKPFEEKKVREYNFR
                     RPVKFSREQQRTLQLVHENFARELSTYLSGRCRTFVDINYASIDQITFSEFQQSLTSP
                     TYIAVFSTSSLAGNAILQVGLDIGYAMIDRLLGGPGSTLEEIRTPTDVETAILRKESS
                     VMLGALSKAWSTIEPFEVSLEKIETNPQFIQIAPPNEMTILVTLSITIRDVQGFMNIC
                     FPSSTLEPISDKLSTRMWTQTAKKSPEVQEKLKNMLMLSKLDLTAVLGKTDICISDML
                     KLQIGDVVRLDSFYQDPIDFDVQGVSIFKSKVGTNKGFYAVQIEKENKQLLEKTFLTK
                     VLNEKFKTDKPREGGSDKGGM"
     gene            5127..6320
                     /gene="fliY"
                     /locus_tag="PLU28_00550"
     CDS             5127..6320
                     /gene="fliY"
                     /locus_tag="PLU28_00550"
                     /inference="COORDINATES: protein motif:HMM:NF005995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch phosphatase FliY"
                     /protein_id="HOG33582.1"
                     /translation="MLGDEFLTQDELNSLLNGLNSDEVKQPDNNPLLDMMGEIGNIAM
                     GAGSTTMSTLLRKKVSITSPDASIIKFKDIKTKFSGQQLVITINYKKGLEGLNTFVMP
                     ARIAQIIADLMMGGNGDLPAEVQLDELGMSAAGEAMNQMMGSAATAMAEFIKTPVDIT
                     PPTVSILDFDNGDTKFPPIESNDEAEIICIKFSMEIKEIATTTVWQFIPMHFAKHIES
                     LMKGPETPAFQPPPAKTQQIPVQQPVQQIPVQQPQQQYQPQPQYYNVPVQQPMYQVPQ
                     GFSNPNIMQGSEVKVNPVNFGPLTGQPSPATESIDLSKLQLLMDVPLELTVELGRVKM
                     SLREILQLYEGSMVQLDKLAGEPLDVYINGRLIARGEVVVIEESFGIRIIEIVSLEER
                     IRTIK"
     gene            6317..6991
                     /locus_tag="PLU28_00555"
     CDS             6317..6991
                     /locus_tag="PLU28_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG33583.1"
                     /translation="MKQKAFIFVCLAVLCNICMGSYQNVFIEYLSNYSGKSCIHADIS
                     VMFNIKQVPRSITADLYMVNSKYIVYELKEPEIFSGIFYVYDIYNGIFYTKNNEEVDA
                     YEEISIITASIPNILNSILIAFQPDKFNFYEKIEDNFLNFYFEPKSKNFLQLFARVDY
                     VKFKAQFFSPYKGVYVFDSFYVLNSDDTKNIKISINKIDYPDEAQASKILESYFSKSL
                     SRSESK"
     gene            complement(6952..7689)
                     /locus_tag="PLU28_00560"
     CDS             complement(6952..7689)
                     /locus_tag="PLU28_00560"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOG33584.1"
                     /translation="MYEKSGKINIFWFENGASNICGLELDDEIVVFDSSLYSKKFEEA
                     IDILQDKYNKKVKKVFLTHYHPDHSFGVLFSKRNIELFMNLETFYCLNSIKKESLKIL
                     SENSGSDFMNLQQLLTKKKVELFNGNILKSFRNTIINGISVGGHSKDSTIYIIKPDNL
                     LISGDLVFSNVHAETQGANIDIWKDLLCKLDTYDIDYVVPGHGTPSGKELLKVQFQYL
                     QDYSNKEKLKKLYSTYLLSDLLKDFEK"
     gene            7810..9540
                     /locus_tag="PLU28_00565"
     CDS             7810..9540
                     /locus_tag="PLU28_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG33585.1"
                     /translation="MLLKDFLKKYWWRYSIGVLFLIAVDLIQIIIPKKVGEIVDILKT
                     PSPDLQLILTIVTGVVFLAIGLSLGRFLWRVTIIGVARLFEFKTINKMFFHIMDLDQD
                     FYDRWRTGDLMTRFTSDTQTVMRMLGFGVIMLVDTLVVTVMTVLAMGEFVSWKLTLLS
                     IIPIPFIAIVSVFFGRIIHKRFTKLQECTSELSNITEESVSGIQVVKVFSNQSTMKDI
                     FNARSKKFYDSQVSLTRVWGLMFPLINFLGAFSSLLVFFFGGKMVVNGEITLGNFVMT
                     NSYVGMLIWPMMAFGWLMNILQSGRASLKRIKEVLNQQKSIVEKNDSIKDGFSGLYSF
                     KNFSFSYPNTERIVLKDINITIEPGQMIALVGKVGSGKSTLAKLMAKLYPVDNGMLYL
                     DSHDINDVDGKYIRENVSYVPQESFLFSMTIRENIAFSDKKIENRVGEYAAMASVDED
                     IKRLAQGYETVVGERGVTLSGGQKQRVTIARALAKDSRVIILDDCLSAVDTETEEAII
                     KSLRKETMGKTVVIVSHRLKAVKNADQIFVFDNGSIIESGTHNELLRKKGLYNSMYEK
                     QLIEEKLKEV"
     gene            9543..11486
                     /locus_tag="PLU28_00570"
     CDS             9543..11486
                     /locus_tag="PLU28_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG33586.1"
                     /translation="MASAYEQIFEEEREKDLGDVLLFKWLWKYIKKYAAILAIAFISL
                     GLTTAVDLSVPNIIRYGIDNLLNVQYKFNLVGETFVYSENGDYTALVEDHGYYMVKDG
                     EKTAVSDVYYKDASSKSISRINSFAFLFLSLLMVQLLFNYGQVFFSNLLGQKVIYDIR
                     SELFGHVEKIKYDFFTKNPSGKITTRIVNDTQNISEFFTEVLTSLTKDIAILTGVVIL
                     MFRLNIKLSGYVVITFPIVLIATYIFRRFDRKAYNKLRTRVAAINAYLAENISGSTVT
                     KLFNQEKRKKDEFNDINFKLYRSKLEQMFVFAVFRPIMTLLYYITLCILLWFGSKGVR
                     DGFVSFGVLYAFTAYIDMFFNPLFDIAEKYDIMQGAFASAAKIYKLLKQEEEEPGKCL
                     YKDIEKGSVTFKDVWFSYSSDHIEDKDHEYVLKGASFTIKESERVAIVGETGSGKTTT
                     VNLINGLYKFQKGDILLDGKPLFDYDVSNLRRRIAVVPQDVFLFSGNITNNIRLFNEN
                     ITKEQVEQAAKKVYADEVIKKLPQAYDSELLERGSTLSSGERQLIALARAVLFDAKII
                     ILDEATANIDVETEYLIQKALNDLSADKTIISIAHRLSTVKSSKRIIVVHKGKVAEEG
                     SHDELMAKKGIYFDLYRLQFENS"
     gene            11556..11993
                     /locus_tag="PLU28_00575"
     CDS             11556..11993
                     /locus_tag="PLU28_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG33587.1"
                     /translation="MEFSLDQIIQIMLAKLENVEMSLEDLKFRTNIALRVLRKSGALT
                     DEELKASIKDEFTAMVEIEGKKVEVPQEKVDEVAKSIIQWVDNDLDAMKKKVEEYQKQ
                     VEDMMAKEKGSADISVAPPDLLNRLDAAKKTQNKQGKGGIILS"
     gene            complement(12024..12524)
                     /locus_tag="PLU28_00580"
     CDS             complement(12024..12524)
                     /locus_tag="PLU28_00580"
                     /inference="COORDINATES: protein motif:HMM:NF014844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HOG33588.1"
                     /translation="MEKTLRQKKVLESISSSTDPVSGSELALKFGVTRQIIVRDVMIL
                     KAQGEDIRSNSRGYYINSEKKHIKTVAVRHSMSEIRDELLIMVNNNATVVDIVVEHPV
                     YGEITGAIGVSTVDDVQAFCARIKNSKPLSSINKGVHIHHIIVEKESDFQNIIEQLEK
                     HKILLK"
     gene            complement(12524..13792)
                     /locus_tag="PLU28_00585"
     CDS             complement(12524..13792)
                     /locus_tag="PLU28_00585"
                     /inference="COORDINATES: protein motif:HMM:NF012312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG33589.1"
                     /translation="MTLDNFIEQNVSRKKRLFLLIISSIGWALVAAGVMVTSLTINDI
                     VAYWKLSKVFSAVMASSTFFGMIAGAVSSGLISDTFGRKNSCVIFSSIFTLFSFSGGI
                     ASTPEMFLAFRIIAGFGMGGLLPALNTYLAEFLNISIRGMYLVLLEASWAVGSIILGV
                     FHLLFKNTLTWRGDYLFFISGLIPLIFIMFMHESPKYLLIKKRYDKIQKLYNTDGSDI
                     ILPVNSKKYRVSDLFRDNHMKITLNVWLLWFTMSIGYYGIFVWIKSILISKNISVLSA
                     DWYTFYMFVAQLPGYLLAALLIEKVGRRKSLLFFMIGTGVSSLAFAFVSTQIQVLIFS
                     LIVSVFCLGAWGITYAYTPELYPTHIRGIGNGSASAITRFAGFLAPYYTSFFLEHSIT
                     VGLVGIAILFVITGILNFLFLPETMNKEVN"
     gene            13961..15061
                     /gene="nagA"
                     /locus_tag="PLU28_00590"
     CDS             13961..15061
                     /gene="nagA"
                     /locus_tag="PLU28_00590"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HOG33590.1"
                     /translation="MKIRGNIYYKGKFEKRTVEIEKGRISSFSESKAELDFGNDIVVP
                     GFIDSHIHGCNGSDVMDMSSKSFETIREFCFSRGVTTPVYTTLSASYNDLKKVVEESK
                     KCDFEVLIHLEGPYINPLKKGAQNEKYIRKPDIAEIKELQRLAEGRIKLITFAPETDI
                     NFEFSAQLKKLGIVPSIGHTNADAQMIQRAHESGIRRFTHYCNAMNGLHHREIGAVGA
                     AMLFDDTIIEIIADGYHLSPDAVKLFYKVFSNSRIIGITDSMRATGLKNGTYDLGGLQ
                     VNVSDGKATLENGTIAGSTLTMDSSLRNVLKFTGTSVEKAIRLYTSNPARNIGLKDRG
                     SIACGKLADLVVLDKEYRVKTVIKTGKVVYSN"
     gene            complement(15058..15675)
                     /gene="nth"
                     /locus_tag="PLU28_00595"
     CDS             complement(15058..15675)
                     /gene="nth"
                     /locus_tag="PLU28_00595"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOG33591.1"
                     /translation="MKNINEQAQIIIQNSSYDFNHEKDPFVVLIKTIISQRNRDEVTE
                     KVSNIFFLKFKNVAEVSRADTAEVESVLKVSGMYRQKSARIIQISKILIEKYNGKVPD
                     SLEKLLELPGVGRKTANIVLYNSFNIPALAVDTHVHRISNRLGWVSTKTPEKTEEALT
                     KLLKKEFWGPVNEAMVYFGKTICRPLSPKCVSCFLKQSCPSAKLP"
     gene            15805..16290
                     /locus_tag="PLU28_00600"
     CDS             15805..16290
                     /locus_tag="PLU28_00600"
                     /inference="COORDINATES: protein motif:HMM:NF014517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HOG33592.1"
                     /translation="MDKILNDLSAVLSQKRLTFGFAESCTGGMISSLVTEIPGISEYF
                     YGSVVSYSNSIKTCLLKVDSHTIELYGAVSRECVEQMADGLIDLMNVDCAVSVSGIAG
                     PGGGSAEKPVGTVWFCFIVDNRRKTEKKFFSGNRQEIRKSASYYAITVMTDMMKAFKE
                     G"
     gene            16293..18383
                     /locus_tag="PLU28_00605"
     CDS             16293..18383
                     /locus_tag="PLU28_00605"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HOG33593.1"
                     /translation="MGEMDVYLSVFLDEARENLQNLNDALLGLESDMKNLEKVNEIFR
                     IMHTLKGMAGTLGFDGLAKLCHTMENTFDKIRNHQMTVNEDIIDLLFKGLDTLGESLD
                     DIASGGNGDTQAVKTLKDGFESILKGQKSEQKVEPFKQAQVKEEPIQEKTVSSVDSAF
                     FIEDSTKVILKNVYDKAKERGLNFYFIKVTLSEGVQLKLARAFMIQHKFDEMGCEVVY
                     SDPSTEDIESEKFDRELVFGIVTSVDPKFLAENLKSISEVEKIQIDDFFTSVNKKAVV
                     TDDADGAASEKHVESQNAELSEESEHKDDNPAQPTKKKLKLSQSIRVDINKLDELMNL
                     MAELVIARSRIAETLKKFSIKEVDESLSQLSRITLDLQNVVMKVRMVPVSFVFNRFPR
                     LTRDIAKELGKEINFIMQGEETELDRTVVDEIGDPLIHLLRNSLDHGIESPEERIAKG
                     KPKIGTIRLSARHEGNGVIIEIEDDGKGMDKEKILKKALEKGLVDQTTASSMKDEEIL
                     ALSFLPGLSTKDKATELSGRGVGMDVVKSTVESLKGTVTLESEKGRGTIVRIRMPLTL
                     AIIEALLVTVKDYVYAIPIANIDTTQKIADGEIKVVQDREVFMLRGEVIPVIRLRELF
                     GYSRTENLKQENIVIIKVGNKKYGVIVDKLLGQDDIVIKSLGSMLSDVKEFSGGAILG
                     DGRIALILDVSSLM"
     gene            18400..18819
                     /locus_tag="PLU28_00610"
     CDS             18400..18819
                     /locus_tag="PLU28_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOG33594.1"
                     /translation="MKDVLSFQIHNQEFAVEVDLIEIVSDMADITPIPNSRQSIKGVI
                     NLRGRIVPIVDITDILRLNLPSDHVYRNILVLKVDEEEIGILVDLVNNVLSIDETKLE
                     NLQSRNEFYAGKVNGVIKKDNRLIVFLDIRAVLGMEE"
     gene            18845..19210
                     /locus_tag="PLU28_00615"
     CDS             18845..19210
                     /locus_tag="PLU28_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG33595.1"
                     /translation="MGKRILIVDDAAFMRMILKDIITKAGYELAGEAQNGQEAIDKYK
                     ELKPDFVTMDITMPVMDGIQAIKQIKTIDPNSRVIVCSAMGQQAMVIEAIQSGARDFI
                     VKPFQPNRVIEAIQKLEQG"
     gene            19207..19608
                     /locus_tag="PLU28_00620"
     CDS             19207..19608
                     /locus_tag="PLU28_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HOG33596.1"
                     /translation="MIPTDSTTYIPTSTVAPTASGGSFILVWILTLVLFVVLLWLLYM
                     FLAKYTKGTKNNPQIRVMKRFYIDRNFYVGLLKLFDEYYLILFSSNSSELLKKLTYEE
                     ALEIIGEKQTFFKTFAGILKKGSEENETKTK"
     gene            19589..20368
                     /gene="fliP"
                     /locus_tag="PLU28_00625"
     CDS             19589..20368
                     /gene="fliP"
                     /locus_tag="PLU28_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209197.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HOG33597.1"
                     /translation="MRLRLSSKIILLTLMVLAFSTSISSAPADLGLTLPNIDIKVGSS
                     EQNLTPAITIFLIITVLSLAPSFLMLMTSFTRIAVVLSFVKQAIGARQAPPNQVVVAL
                     ALFLTILIMFPVFSNVYNKAIVPYNSGTIGYEKAVERTWNEFKTFMLREIVNHKNQDN
                     IYMLAQSTNIEIKDINDTPFQVLIPAFALSELEISFKMGILIYLPFIIVDMVVASILL
                     SMGMMMIPPVMISMPFKILLFVLVNGWDLLIGSLVRSFSGG"
     gene            20371..20637
                     /gene="fliQ"
                     /locus_tag="PLU28_00630"
     CDS             20371..20637
                     /gene="fliQ"
                     /locus_tag="PLU28_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HOG33598.1"
                     /translation="MSEEVFIEVFIEGIRIFLVTISPILLVSLGVGLIISIFQTVTSI
                     QEQTLTFAPKIIVTFLALALMFGWIIQQIGDYAYRLFTTYMGMI"
     gene            20638..21405
                     /gene="fliR"
                     /locus_tag="PLU28_00635"
     CDS             20638..21405
                     /gene="fliR"
                     /locus_tag="PLU28_00635"
                     /inference="COORDINATES: protein motif:HMM:TIGR01400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HOG33599.1"
                     /translation="MYFYDYQLWIYFFILIRIVGVSFFIPFFSTSLLPTPVKIFLVIF
                     VSYICLPMSTQTMPLNTDALTIVFYLLLNFTVGISIGIITQIIFSAVQFAGDFFGIQM
                     GFALAEVLNPVYEEEVSLFSELTFLLTTMIFFIFKGHIWLYTLIIESFKKLPVVFSLN
                     QDVFMVLSQKLGDVMIIGVEFALPITAFMLLTNISLGIASRLMPQLNVFMIGLPMNII
                     IGFLIFTVVIYTWEENFTGLFFQIYTWLQSTITMFAK"
     gene            21327..22469
                     /gene="flhB"
                     /locus_tag="PLU28_00640"
     CDS             21327..22469
                     /gene="flhB"
                     /locus_tag="PLU28_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HOG33600.1"
                     /translation="MGRKFYRTFLSDLYLASEYDNHVCKIDLQFFADPSKTEEPTQRK
                     LEKAREEGNVPVSREFTTGITFLALAGILSFLGGPIAKELEKLFYDYFDMNTDLLNPS
                     FFIYSLGTHSKLYVFIALFFGMGMAVTLGLGFIQTRFMIAPKALKFDLSKINPIKGFK
                     RIFSMRSLFELFKAILKIIIIGWVAYSIISGKMNDITNLAGSEIAGSVKFIVNMILEL
                     MYKLGLSLLILSLADYWFQKFQYRQDLKMTKQEVKEEMKDIEGNPEIKRKQREFMTKI
                     VFSRMMQQVPTADVVVTNPTHYAVAIKYESKGMNAPKVVAKGVDEMAMRIRKVASENG
                     VPIVQKPSLARELYAKVELDEEVPQDLYKAVAEVLAYIYKLSNKNV"
     gene            22479..24599
                     /gene="flhA"
                     /locus_tag="PLU28_00645"
     CDS             22479..24599
                     /gene="flhA"
                     /locus_tag="PLU28_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HOG33601.1"
                     /translation="MNFIKNLDVVFSVLIVVMVLLMVLPIPTFLLDFLQLFNIALSII
                     ILLSTLYVKRALDISSFPSLLLITTLFRLALNVSSTRLILLKGPKFDGKVIRAFGDFV
                     VGGNYIVGIVIFLILVIIQFIVITKGSERISEVAARFTLDAMPGKQMSIDADLSSGLI
                     TEDEAKTRREDIRREADFYGAMDGASKFVRGDSIAGLIITIVNILGGILIGILQQGIS
                     IQEAAGLYTLLTVGDGLVAQIPSLLISTASGMVVSRAASKESFGSNIIKELTSEKKIL
                     SITGFMLIAIGVFTPLPTAPSLVIGGVMLFVAYSATAGQKKKLAYQTAGGSMQSKNIL
                     SQQNEASGGQSVPSKSVKSAPPLTSADEVSEVIQSDTIEVDIGYGLIPLADPTQGGDL
                     LDRVTMVRKQIAYELGVVIAPVRIRDSVLLSSNEYIIKLRGVEIGRYELIPNRLLAIN
                     SGMANMALAGVQSKEPAFGLAAYWIDEGLKDDAISMGYTVVDAPSVFATHLSELIKRY
                     AHEIVGMKELEMLIDGLRVKNATLVDTLIPALLKLHELRKVIQDLLYERISIRNMPMI
                     FEQLIETSDKSGKDQEKLVEDVRVALGRQICESLKSSDGQFHVIALDASIERKILDSF
                     TDGENGRLLILEPEYANLLIERVSKGLEEIMMKGYNPVLICSKSIRYQLAKLILRFIQ
                     NINIVAYEEVPSDVALSVEGIVNF"
     gene            24614..>25690
                     /gene="flhF"
                     /locus_tag="PLU28_00650"
     CDS             24614..>25690
                     /gene="flhF"
                     /locus_tag="PLU28_00650"
                     /inference="COORDINATES: protein motif:HMM:TIGR03499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HOG33602.1"
                     /translation="MKVKKYIVNDLAEAMTKIKDDFGDDAYILETKKIKKGGFIGFGG
                     KKFLEVTVLSEKEEEKARNQVSNGNKEKADTFESDLYDMKSVISNNKRLEKRIKQESM
                     AAQRDIPQEYEVQNTEKEYNKDFIELIKQQRELSKSMDNSAKDFYEQQNVTAERPVKN
                     IKYDTYTRNQVIPETFAGAAAEKASQVNNDSVSEIKKMLSELNKKVSGESEYINDFRQ
                     ALLKSDFSDEIIDEFLSQQTGNIKEDWKSNVQLREAFTKYLAAIIKKDKQQFKDKIIL
                     VGPTGVGKTTTLAKISAILKKQGKKIAIVTVDTYRIAAADQLKIYADIMGVPAFICYT
                     PGDLRSTLQTLVGYDAVLIDTAGRS"
BASE COUNT         8284 a         4383 c         5505 g         7518 t
ORIGIN      
        1 ctacaccagc cagcttggat gcttctaaac aggcagtgaa gtttgagttt gatggatcag
       61 ctacaaatac aaaggtaaaa gtaacggtag ttgaagaagg aaaagagtat tctgatactt
      121 atgagcttgc tgataaccaa gtttcaacta taaatgcatt ctttaacggt ggtactgatg
      181 taggaggcaa aactcttata ataaacggta aacagcttaa agctgccgct cttataagcg
      241 gagctgcgac agacagagtt aacataaact ccatcacatg gcctaccatg gttgacggta
      301 ctcttgcagg aacttctggc ggaacagcca gggacttttc acttgcaggc ggtataaagc
      361 ttacggatac cgttaaagga caggatattt actatgatcc gaaaaacgtt aaagttacag
      421 ttactaatgc tgctggagcc atacccggaa gtgtaacggt ttctcttacc acaaaggatg
      481 gtgtaaagaa ctttacaata aacggaacgg tagatgcagg agttggaaag aacgtttctt
      541 accagcagtt taacgaagcc ttcagcgatg gcgtaaagaa cggcaacctt aggataaccg
      601 gtctcagcct aagcaatggg caggatactg atgaacttac ggcaacggct gctacaggag
      661 ttacgtttgc atcggtaaaa aacattatac agccgccttc cggaggatca cctaagtttg
      721 tagatgtcaa taatcctcag aactttgttg tggccggata taaggctcct acagttgcag
      781 cgtccaccct cgtatatgat tcgcttggac aggcttataa cgtatacctt aattttacaa
      841 aaataaatga aaatacatgg ttctggaaag ctcagatgga ggacggaaca cctctttaca
      901 agcttaatga agacggtaca agagatatga caagctttgc cgacggtgta atagcttttg
      961 acggcaatgg cggtattatg agcactaatt ggaggctaag ggaagacgga acaatagata
     1021 ccaataccac agataacaat aacggttcac taggattctg gtttgatcca gctaaaccag
     1081 gagcggcacc taatcctacc tataatccca gttcgatctc agcagccgga cctgtgatgg
     1141 cacaagtaaa gtttcaagag ttgacgcagt ttgcttctcc gcattcgata gcggttactg
     1201 aacaagatgg aaattctgaa ggtactcttg attcttttgc tataaatgaa gtcggacaga
     1261 taataggaac ttttaccaac gggaagtcgg atgctttagg tcaggttgcc cttgctgttt
     1321 tcaataaccc ggctggtctg cttgaacttg gaaactctct ttacgcgcaa agctctaaca
     1381 gcggaattcc tcagatagga acctgcgggg tgggaggacg cggaacattg cttccaggaa
     1441 ctctggagat gtccaatgtt gaccttgcag aagagtttac caacatgatc atagcccagc
     1501 gaggattcca ggccacatca agaataatta ccacagctga tcagatacta aacgaactgg
     1561 tcaacataaa gaggtagtat gaagtttttg gaggtgacgt atgattagtg tgacaagact
     1621 aaataacgaa ggatttgtga taaacggcga acttatagaa aaacttgaag ccaagcctga
     1681 tacaacgata actcttttga acggacatgt ctatattgtc aaggaaaagg ttgaagaagt
     1741 cattaaaaaa gtgatagaat ataagaagag tatatttata ggcccgcaaa aggagtgaaa
     1801 caataatgga tatttcaagt cttttaggtt tggctcttgg aatcatagct tttgttttcg
     1861 gagtgtattc atctgccggc tacagtttca gtggactgtc ggcaatgatc gatgttccgt
     1921 caatgatagt tactatagta ggatcaatag gctctatgtt catttcaaat ccgaaagaaa
     1981 aggctttcaa atttgtatct tatataatga tggcacttaa agaacagaag atagatatgc
     2041 taggtacact cagaactctt tacacctttg ctgaaaaagc cagaagagac ggcatgattt
     2101 ctcttgaaga ggatataggt aagattgatg atgagtttat gagagacggt cttcgagcag
     2161 ccgttgatgg taccgatcca gaagagatcc gtaagatact ggagataaag atggagcttt
     2221 atgaggatga ccaaggtggc aatattgctg taatggagtc atggggggca tatgggcctg
     2281 cttttggtat gatcggtact cttataggac ttatactcct tttgggacag cttaacgatc
     2341 cgacagcgat aggtccgtat atgtcggttg ctcttatcac aaccctttac ggagcattga
     2401 ttgcaaatat tcttgcaacg cctgttgctg ataagctcaa aaagagaacc gcaaaaatga
     2461 taagtatgaa gagaatgact acagaaggaa tactttcgat agttcagggc gaaaatcccc
     2521 gtcttatgga agaaaaatta aaggcgttct tatctgcgca ggatagggca acatatgaaa
     2581 aaggaaaagg cgaagcacag gcctgagctg gaggtaaaaa atggggaagg acaaatgcaa
     2641 ctgcccgcca ccggctcctg tgtggctaac aactttcagt gatctgaata atcttttgac
     2701 aaccatgttt atagctcttt tttctatggc cactatatct cccggtaagt ttcagcaggc
     2761 tgcatcaagt ttggcgagtg cgtttaacgg tcagccaatg ggagtgcttg tcggcggtaa
     2821 aacgattagt gaggagccgc ttatcacttc gaatccaggt gtaaaggaaa accttcttaa
     2881 gattcagatg gatgaaaatt ttaaaggtat gatcactata gaagaaactg acaggggtac
     2941 cataatacat ataaaggact atgcattctt tgaaacaggc agtgcacagc ttacaaaaaa
     3001 agcaaaggag ctgttgtatc tcataagtac aatcatactt gaacatactt caaacgatat
     3061 agaagtatat gggtatactg atgaacgagc tgttttatcg actagtgtat atccttcgaa
     3121 ctggcatctt tctaatgccc gcgccgccag tgtagcttac tttataacag gggagcttaa
     3181 ggataggcgt cttgttgaac ttgtaggtca gattaaaagc ggtcagtttg atatcaatta
     3241 cttttatgaa ccaacaaggt tttttccgat aggaagggga gatgctgata ttcttaagga
     3301 gatggatgag cttaaaattg ccaatgataa ccgtcgcgat atccttagcc ttgactttca
     3361 gaaaggttcc atgacagcgc aggagttaaa ggataagctt gcttccatcg ataaagagta
     3421 tgaagccgca aaagaaaaac taagagaaga ttatagaaga gtagacttac ttatacttag
     3481 acaaagagta agataggagg attgattatg gctcaggaaa atccggtacc ggaagagggt
     3541 aagaaaaaag gcggaaataa ttttttgctg atgataataa tagtcgtcgt agttactctg
     3601 gtggtttctg caggaacgtc attcctgata ataacgcttt tgggacaaaa tgtacaaaaa
     3661 actgcagatg cgacaccttc tgccggtact actggcacta caggcttggt ggttcaggcc
     3721 gaaatgatca gggaaggcgc acgttatcct gtgatgctca aaggcggaaa ggatgttgcc
     3781 attgttgatg cactgtacta taaagttgga agcgaagaat gccgttcttc aataggtaca
     3841 aataaactgg aaatacttga tgccgtaagg acgatctttc ttaataagac ttcttcggaa
     3901 gttacaaatc ctacaggtca ggaacttata aaaaaacaga taaaagatgc tgtaaatgag
     3961 ataaccggat atacaggaga aagagaaaaa ataggggttc tgagtgttat tcttataatt
     4021 cttacaatct cccagaatca atagagaggt gcctatatgg tcgaaggcga taagctgtct
     4081 caggatgaaa ttgataagct gttaaagaac atgacagccc ctgagggcga gagtgaagga
     4141 tctgaaaaac cctttgaaga aaaaaaagta agagaatata atttcagacg tcctgtaaag
     4201 ttttctagag aacagcagcg tactctgcag cttgttcatg aaaattttgc acgtgagctt
     4261 tccacttatc tttcaggaag gtgcaggaca ttcgtagata taaattacgc aagtattgat
     4321 cagataactt tttcagagtt tcagcaatct ttaacctcgc ctacatatat cgcagttttt
     4381 tcgacatcct ctcttgctgg taacgcaata cttcaggttg gtcttgacat agggtatgct
     4441 atgatagaca ggcttcttgg cggacccggc agcactcttg aagagataag aacgcctact
     4501 gatgttgaga cggctatact cagaaaagaa tcttccgtca tgctgggggc tctttcaaag
     4561 gcatggagta ctatagaacc ttttgaagtt agccttgaaa aaattgaaac caatcctcaa
     4621 tttatacaga tagctcctcc gaacgaaatg accatacttg tcactttatc tataaccatc
     4681 agagatgtgc aagggtttat gaatatttgt tttccttctt ccacacttga acctataagc
     4741 gataagcttt caaccagaat gtggacacag acagcgaaga agtcgcctga agtacaggaa
     4801 aagcttaaga atatgcttat gctttcaaag cttgacctta cggcagtgtt gggaaaaaca
     4861 gatatctgta tctctgatat gcttaaattg cagataggtg atgtagtgag gcttgattct
     4921 ttttatcagg atcctataga ttttgatgtt cagggagtat ccatcttcaa gtcaaaagtg
     4981 ggaactaaca agggttttta tgcagtgcaa atagaaaagg aaaacaaaca gttgcttgaa
     5041 aaaacatttc ttacaaaagt actaaatgaa aaatttaaga ccgataaacc ccgtgaaggc
     5101 ggttcggaca aagggggtat gtgattatgt taggagacga atttttaacc caggatgaac
     5161 tgaactcttt gcttaacggg cttaattcag atgaggtaaa gcagcctgac aacaatccat
     5221 tgctggatat gatgggtgaa attggcaaca tagctatggg agccggttca acaaccatgt
     5281 ccactcttct tagaaaaaaa gttagtatta catctccgga tgcaagcata ataaagttta
     5341 aagatataaa aacaaagttt tcaggtcagc agttggttat aaccataaac tataaaaagg
     5401 gtcttgaagg tttgaatact tttgttatgc ctgcaagaat agctcagatt attgctgacc
     5461 ttatgatggg cggcaatggg gatcttcctg cagaggttca gcttgatgag ctgggaatga
     5521 gtgcagctgg cgaagccatg aatcagatga tgggttcagc agcaactgcg atggctgaat
     5581 ttataaaaac accagtcgat atcactccgc cgactgtaag catacttgac tttgataatg
     5641 gagatacaaa atttcctcct attgaatcaa atgatgaagc tgagataata tgcataaagt
     5701 tcagcatgga aataaaagaa attgcaacga ctaccgtgtg gcagtttata ccaatgcatt
     5761 ttgccaaaca catagaaagt ttgatgaaag gtccggaaac accggcattt cagccgccac
     5821 cggcaaaaac acagcagata cccgttcaac agcccgtgca gcagatacct gttcaacagc
     5881 cgcagcaaca gtatcagcca caaccgcaat attacaatgt tccggttcag cagcctatgt
     5941 atcaggtgcc tcaaggtttt tcaaatccaa acattatgca aggaagtgag gtaaaggtga
     6001 atccagtcaa ttttggtcct ttaacgggtc agccctcccc tgcaacagag agtatagatt
     6061 tatcaaagct tcagcttttg atggatgttc cgcttgaact gacagttgaa ctgggaagag
     6121 tgaaaatgtc tttgagagag atacttcagc tttatgaagg atcgatggtt cagttggaca
     6181 agcttgcagg agaacctctg gatgtctata taaacggaag gcttattgca agaggtgaag
     6241 ttgttgtaat agaagaatct ttcggtataa ggataattga gattgtatct cttgaagaga
     6301 ggataagaac tataaaatga agcaaaaggc attcatattt gtttgtttgg cggttctttg
     6361 taatatctgc atgggctctt atcagaatgt ttttatagag tatctttcaa actattccgg
     6421 taaatcctgc atacatgccg atatttctgt tatgtttaat attaagcagg tacctaggtc
     6481 aataacagcc gatctgtata tggtaaattc aaagtatatt gtatatgagt taaaagagcc
     6541 agaaattttt tctggcattt tttacgtata cgatatatac aacggtattt tctataccaa
     6601 aaacaacgaa gaagtagatg cttatgaaga aataagcatc ataacagcaa gcataccaaa
     6661 tattctaaac agtatactta tagcctttca gccagataaa ttcaattttt atgagaaaat
     6721 tgaagataac tttcttaact tttattttga acccaaatcc aaaaattttt tacagctgtt
     6781 tgcacgtgtg gattacgtaa agtttaaggc tcagttcttt tccccgtata aaggagtgta
     6841 tgtttttgac agcttctatg ttctgaattc agatgatacc aagaacataa agataagcat
     6901 aaacaaaatt gactatccgg atgaagcaca ggcgtcaaaa atattggaaa gttatttttc
     6961 aaaatctttg agcagatctg aaagtaagta ggttgagtaa agttttttaa gcttctcttt
     7021 attggaataa tcctgcagat attgaaactg aaccttcaac agttcctttc cagaaggggt
     7081 tccatgtccg ggaaccacat aatctatatc gtaagtatcc aatttgcaaa gcagatcctt
     7141 ccatatgtca atatttgctc cctgagtttc ggcgtgcacg ttagaaaaaa caagatcgcc
     7201 ggatataagc aggttgtcag gctttatgat atatatggta ctgtcttttg aatgtccgcc
     7261 tacggagata ccgtttatta tagtatttct aaaggattta agaatatttc cgttgaaaag
     7321 ttccaccttt tttttcgtaa gtaactgttg cagattcata aagtctgatc ctgagttctc
     7381 tgaaaggatt ttcaggcttt ctttttttat agagttaaga cagtaaaacg tttccaaatt
     7441 cataaaaagt tcaatatttc ttttcgaaaa aagtactcca aaggaatgat cagggtggta
     7501 atgagtgaga aatacctttt taactttttt gttgtactta tcctgtagga tatcgattgc
     7561 ctcttcaaat tttttgctgt aaagagacga atcaaatact acaatctcat catccagttc
     7621 gagtccgcat atattgcttg ctccattttc aaaccaaaaa atatttatct ttccggattt
     7681 ttcgtacatt tcatcgacct ctctattatt tgtattcatg ttatttttct acaaaattat
     7741 ttacatatcc ttaaatagat ggtataattt tttcagttaa taaaacaaac caaccaacgg
     7801 ggtgatttaa tgcttttaaa agacttttta aagaagtact ggtggaggta ctccataggc
     7861 gtactcttcc tgattgcagt ggatttgata cagatcatta tccccaaaaa agtcggtgaa
     7921 atagtggata ttcttaaaac accttctccc gatcttcaac ttattttaac tattgtgaca
     7981 ggggttgttt ttttagccat aggtctttca ttgggaagat ttctatggag ggttacgata
     8041 attggggttg caagactttt tgagtttaag acaatcaata aaatgttttt tcatataatg
     8101 gatcttgacc aggattttta tgacagatgg cgcactggag accttatgac caggtttact
     8161 tcagatactc aaaccgtaat gcgaatgctg ggttttggag taataatgct ggtagataca
     8221 ctggtcgtta ccgtaatgac agttcttgca atgggagagt ttgtgagctg gaagctgaca
     8281 ttgctttcaa tcatacctat accttttata gcgatagtat cggttttttt cggaagaatt
     8341 attcataagc gttttaccaa gcttcaggaa tgcacttcag agctctcaaa tattacggag
     8401 gagagtgttt ctggcataca ggtagtaaag gttttttcaa atcagagtac catgaaggat
     8461 atttttaacg ccaggtcaaa aaaattctac gattcccagg tttcacttac cagggtgtgg
     8521 gggcttatgt tcccactaat aaactttctc ggagcctttt caagtctgct tgtttttttc
     8581 tttggcggca agatggtggt gaacggtgag ataactctcg gaaactttgt tatgaccaac
     8641 tcatatgttg ggatgcttat atggcctatg atggcttttg gatggcttat gaacatactc
     8701 cagagcggaa gggcttccct caaacgtatt aaagaggttc ttaaccagca aaaaagcatt
     8761 gtcgagaaaa atgactctat aaaagatgga ttttcaggtt tatacagctt caaaaatttc
     8821 tcattcagct atcccaatac cgaacgaata gtgctgaaag atataaacat aacgatagaa
     8881 ccaggtcaga tgatagctct tgtcggcaag gtcggttcag gtaagtcaac tctggccaag
     8941 ctgatggcca agctttatcc tgttgataac ggtatgcttt atctggactc tcatgatata
     9001 aacgatgttg acggaaagta tataagagaa aatgtttcat atgttcctca ggaatccttt
     9061 cttttctcta tgactataag agaaaatata gctttcagtg acaaaaaaat tgaaaacagg
     9121 gttggagagt atgccgctat ggctagtgta gacgaagata tcaagagact tgcacagggc
     9181 tatgaaaccg ttgtcggaga aaggggagtc acactttccg gaggacagaa gcaacgtgta
     9241 actatagcca gggcacttgc taaagactca cgagtaataa ttctggatga ctgtctttct
     9301 gcagtagata ccgaaacaga agaggccata ataaaaagtc ttcgcaaaga aaccatggga
     9361 aagacagttg ttattgtttc acatagacta aaagcggtca agaatgccga ccagatattt
     9421 gtttttgata atggaagcat aattgaatcc ggtacccata atgagctttt acgtaagaaa
     9481 ggtctttata attccatgta cgagaagcag cttatagaag aaaagctcaa ggaggtttga
     9541 aaatggcttc tgcatacgaa cagatttttg aagaagaaag agaaaaagat cttggagatg
     9601 tgcttttatt taaatggttg tggaagtaca taaaaaaata tgctgctata cttgccatag
     9661 cgtttatttc gcttggtctt acaacagctg ttgacctttc ggttccaaat ataatacgtt
     9721 acggaataga taaccttttg aatgtacagt ataaatttaa tcttgtaggt gaaactttcg
     9781 tatactcaga aaatggcgat tacactgctt tagttgaaga tcatggttat tatatggtta
     9841 aagatggtga gaaaacagct gtatccgacg tatactataa agatgcatca agtaaatcaa
     9901 tttcaagaat caattctttt gcctttttat ttctcagtct gcttatggtt cagcttcttt
     9961 ttaattacgg acaggttttt ttctcaaatc ttttggggca aaaggttata tacgatataa
    10021 gatctgagct ttttggacat gttgaaaaga ttaaatatga ttttttcacc aagaatccat
    10081 caggaaagat aacaaccaga attgtcaacg atactcagaa tatctctgaa ttttttacgg
    10141 aagtcctgac aagtctaacc aaggatattg cgatacttac aggtgttgta atacttatgt
    10201 tcagactcaa tataaagctt tccggttatg ttgtaataac ctttccaata gtccttattg
    10261 cgacctacat attcagaagg tttgaccgta aagcttacaa taagctcagg acaagggtgg
    10321 ctgcaataaa tgcctacctt gctgaaaata tatccggaag tacggttact aaacttttta
    10381 accaggaaaa aagaaaaaaa gatgaattta acgatataaa ctttaagctt tacaggtcaa
    10441 aacttgaaca gatgtttgtt tttgctgtgt tcagacctat aatgactctt ttgtattaca
    10501 ttactctttg tatactctta tggttcggtt caaaaggagt aagggatggc tttgtgtctt
    10561 tcggagtact ttatgcattt accgcatata tagatatgtt tttcaaccct cttttcgata
    10621 ttgctgaaaa gtatgatata atgcagggag catttgcctc tgctgccaag atatataaac
    10681 ttttaaagca agaggaggag gaacctggta aatgccttta taaggatatt gagaagggat
    10741 cagtaacttt taaggatgtc tggttttctt attccagtga tcatattgag gacaaagatc
    10801 atgagtatgt tcttaaggga gcttctttta caataaaaga aagcgaaaga gtagccatag
    10861 tcggtgagac tggttcggga aagaccacta ctgttaatct tattaacggt ctatataagt
    10921 tccaaaaagg agatatactg cttgacggaa aaccactttt tgattatgat gtctccaatc
    10981 tcagaaggcg catagcggta gttccgcagg atgtttttct cttctccgga aatataacca
    11041 acaatatacg tcttttcaat gaaaacataa ccaaagaaca ggttgaacag gccgcaaaaa
    11101 aagtttatgc tgatgaagta ataaaaaagt taccgcaggc ttacgacagc gaactgcttg
    11161 aaagaggtag cactctttcc tcaggcgaac gccagcttat agccctggca agagcggttc
    11221 tttttgatgc caagatcatt atacttgatg aagccactgc caatattgat gttgaaacag
    11281 aatatcttat tcagaaggcc cttaatgatc tatctgccga caaaaccata atttccattg
    11341 cgcacaggct ttctaccgtt aagagctcaa agagaataat agtggttcac aaggggaaag
    11401 tagcggagga aggctcgcac gatgaactta tggcaaaaaa aggtatatac tttgatcttt
    11461 acagacttca gtttgaaaac tcataacttt gctgatataa aaaggcaaat tattttaagt
    11521 aaaatatata gtaaaaataa ataggaggta gcgaaatgga atttagtctg gatcagataa
    11581 tacagattat gttagcaaaa cttgagaatg tcgaaatgtc tcttgaagat ttaaagttca
    11641 gaaccaacat tgcccttaga gttctcagaa aatcaggggc attaactgat gaagagctca
    11701 aagcatccat aaaagatgag tttacagcaa tggtagagat tgaaggtaaa aaagtcgaag
    11761 ttccccagga aaaagttgat gaagttgcca aaagcataat acaatgggtt gataacgatc
    11821 ttgatgcgat gaagaaaaaa gtcgaagagt atcaaaaaca ggttgaagat atgatggcaa
    11881 aggaaaaagg ctctgccgat atatcagttg ctccaccaga tcttcttaac aggcttgatg
    11941 cagctaaaaa gacccagaat aaacaaggta aaggtggaat aattctttct taaataataa
    12001 aaaccggcat atgccggttt ttattattta agaagaatct tatgcttttc aagctgttca
    12061 ataatattct gaaagtctga ctctttttcg acaataatat gatgaatatg tactccttta
    12121 ttgattgaag aaagaggctt agagtttttt atgcgtgcac aaaaggcttg gacatcatcg
    12181 acagtggata cacctatggc tcctgttatc tcaccataaa ccggatgttc aactacgata
    12241 tcaacaaccg tggcattgtt gtttaccatt atgagaagtt catcacgtat ttcactcatg
    12301 ctatgcctta cagccacggt ttttatatgt ttcttttcac tgtttatata gtatcctctg
    12361 gaatttgacc ttatatcctc cccctgggct tttaaaatca taacatccct cactatgatc
    12421 tgtcttgtca caccaaactt cagtgcaagc tcactgcctg ataccggatc agtacttgag
    12481 gatatcgact caagtacttt tttctgccgc agggtttttt ccatcagtta acctccttat
    12541 tcattgtttc aggaagaaat aaaaagttta gaataccggt aatgacaaaa aggattgcga
    12601 ttccgacaag acctaccgtt attgaatgtt caagaaaaaa agaagtatag taaggagcaa
    12661 gaaatccagc aaaccttgtg atagcgctgg cggaaccgtt tcctatccct cgtatgtgag
    12721 tagggtaaag ctccggagta taggcataag tgatgcccca tgctccaagg caaaagacag
    12781 atactataag gctgaatatg agtacctgaa tctgtgtaga cacaaaagcg aaagccagtg
    12841 aagatactcc tgttccgatc atgaaaaaca ataggctctt tctcctgcct actttttcta
    12901 tcaggagggc agccagaaga tagcccggta actgagcaac aaacatataa aaagtatacc
    12961 aatctgcaga taagacagat atgttttttg aaatgagaat cgactttatc catacgaata
    13021 taccgtaata ccctatgctc attgtgaacc acaaaagcca gacattcaga gttatcttca
    13081 tatggttgtc tctgaaaagg tcagacactc ggtatttttt tgaatttact gggagtatta
    13141 tatctgaacc atcagtattg taaagttttt ggatcttatc ataccttttt tttattaaaa
    13201 gatattttgg agattcatgc ataaacatta tgaatataag aggaattaat cctgatatga
    13261 aaaaaagata atctcccctc caggtaagag tatttttaaa caacagatga aaaactccca
    13321 gaataatact tccgactgcc caactggctt caagcagaac aagatacata ccccttatgg
    13381 aaatattaag gaactctgcc agatatgtat tcaatgcagg aagaagtccg cccattccaa
    13441 aaccggcaat aattctaaaa gcaaggaaca tttcaggagt ggacgctatc cctcccgaaa
    13501 aagaaaataa agtaaatata gaagaaaaga tcacacagga attctttcgg ccgaaagtat
    13561 cggaaataag gcccgaagag actgctccag cgatcattcc aaaaaatgtc gagcttgcca
    13621 tgaccgctga gaatactttt gaaagcttcc aataagccac aatatcgttt atggtaaggg
    13681 aagttaccat aactcctgct gccacaagtg cccagcctat agaggagatg atgagaagaa
    13741 agagtcgttt tttccttgaa acattctgtt caataaaatt atcaagagtc attttttcct
    13801 cctatgtata tacagtatat acaatatatt atatatcaga tttgcctaaa atacaaattt
    13861 tttgatgatc ctgccgaaaa agtaataaac tctgatataa ttgccatata atagactttt
    13921 gacatatgaa ctattattta aagatattgg agggaaaagt atgaagataa gaggaaatat
    13981 atattataaa gggaagtttg aaaaaagaac agttgagata gaaaaaggac gaatttcttc
    14041 tttttctgag agcaaagcag agttggattt tggaaacgat attgttgttc ctggctttat
    14101 agactctcat attcacggat gcaacggaag cgatgtgatg gatatgagca gcaaaagctt
    14161 tgagaccata cgtgagttct gtttttcccg tggtgtaaca actccggttt atacaactct
    14221 ttctgcgagt tataatgacc ttaaaaaggt tgttgaagaa agtaagaaat gtgattttga
    14281 agttcttatc catttggaag gcccatatat aaatcctttg aaaaaaggtg cccagaatga
    14341 aaaatatata aggaaacctg acatagctga gataaaagag ctccagcgtt tagccgaagg
    14401 aagaattaaa cttatcacat ttgcgcctga aaccgacata aattttgagt tttcggctca
    14461 actgaaaaag ctaggaatag ttccatccat aggccatacc aacgctgatg cacagatgat
    14521 acaaagagca catgaaagcg ggatcagaag gtttacacat tactgtaatg ccatgaatgg
    14581 acttcatcac cgcgaaatag gggcagtagg tgctgcgatg ctttttgatg acacaataat
    14641 agagataatt gccgacggat atcatctttc gccagacgcg gtaaaacttt tttataaagt
    14701 cttctccaat tcaagaataa taggtataac cgattctatg agagctaccg gacttaaaaa
    14761 cggcacctat gatttaggcg gtcttcaggt gaatgtttct gatggcaaag ccacattgga
    14821 aaacggaact atagcaggta gcactcttac catggatagt tcgctaagga atgtgcttaa
    14881 atttacagga acttccgtag aaaaagccat cagactgtat acctctaatc cggcaaggaa
    14941 tatagggctt aaagacagag gcagtattgc ctgtggaaag cttgctgatc tggttgtttt
    15001 ggataaagag taccgtgtaa aaacagtcat aaaaaccggg aaagtggttt atagcaatta
    15061 aggcagtttt gctgacggac atgactgctt aagaaaacat gaaacacatt ttggagatag
    15121 cggtctgcat atggtttttc caaagtaaac catagcttcg tttaccggac cccaaaattc
    15181 ttttttgaga agttttgtca aagcttcttc agttttttcc ggtgttttgg tacttaccca
    15241 tccaagtctg tttgatatcc tgtgtacgtg ggtatcaacg gccagggcag ggatattgaa
    15301 tgagttatac agtactatgt tggcggtttt tctacctact cccggcagtt ctaaaagctt
    15361 ttcaagggag tctggaactt ttccgttata cttttcaatt aatattttac ttatctgtat
    15421 gattcttgca cttttctgcc tatacatgcc gcttacttta aggacagatt caacttctgc
    15481 ggtatcggcg cgggagactt ccgcaacgtt tttgaacttt aaaaaaaaga tgtttgaaac
    15541 tttttctgtg acctcatccc ggttacgttg agagataatg gtttttataa gcactacaaa
    15601 aggatccttc tcgtggttga agtcgtaact gctgttttgt attattatct gtgcttgttc
    15661 atttatattt ttcatttttt tcctcccaaa agtattcatt ataattttat acccaaacat
    15721 ataaaaaata aagattttta aaatagatga tgattttata ccgtttattt gatataatta
    15781 atctggtgaa aggtgtgacg gagtatggat aaaattttga atgatctgtc tgctgtcctg
    15841 tctcaaaaaa ggctgacttt cggatttgca gagtcatgca ccggcggtat gatatcttcc
    15901 ctggtaacag agatacctgg tatttctgag tatttttatg gatcggtagt atcgtattca
    15961 aacagcataa aaacctgttt gctcaaagtt gacagtcata cgatagagct ttacggagcg
    16021 gttagccggg aatgtgttga gcagatggcc gatggtctta tagatttgat gaatgttgat
    16081 tgtgcggttt cagtaagcgg aattgccggg cctggaggag gatcagctga aaagcctgta
    16141 ggaacggtgt ggttctgttt tattgttgat aatagaagaa aaacagaaaa gaaatttttt
    16201 tcaggcaaca gacaagaaat aaggaaaagc gcatcatatt acgcaataac ggttatgaca
    16261 gatatgatga aagcttttaa ggagggatga agatgggaga aatggatgtc tatctaagcg
    16321 tgttcctgga cgaagcgaga gaaaatcttc agaatcttaa tgatgcactt ttgggtttgg
    16381 aaagcgatat gaaaaacctt gaaaaggtaa atgaaatatt cagaataatg catacactaa
    16441 aagggatggc tggcacgctt ggttttgacg gacttgcaaa actttgtcat actatggaga
    16501 acacttttga caagataagg aatcatcaga tgaccgttaa tgaagatata attgatcttc
    16561 ttttcaaagg tcttgatact ctaggggagt ctttggatga tatagcttca ggaggaaacg
    16621 gagatacaca ggcagttaaa actcttaaag atgggtttga atctattctt aaagggcaaa
    16681 aatcagaaca gaaggtagaa ccgtttaagc aagcccaagt taaagaagaa cctattcagg
    16741 aaaaaactgt aagctcagtt gattcagctt tttttataga agactctacc aaggtcatac
    16801 ttaaaaacgt ttacgataaa gctaaagaaa gaggacttaa cttctatttt ataaaagtta
    16861 ccctttcaga aggggttcaa cttaagcttg caagagcttt catgatacag cataagtttg
    16921 atgagatggg ttgtgaggtt gtttattcag atccttccac agaggatata gaaagcgaaa
    16981 aatttgacag ggagcttgtt ttcggtatag ttacttctgt tgacccgaaa ttcttggctg
    17041 aaaatcttaa atctatatca gaagtagaga aaatacagat agatgatttc tttacaagtg
    17101 taaataaaaa agctgttgtt acagacgatg ctgatggagc agcttcagaa aaacatgttg
    17161 aatctcagaa tgctgagcta tctgaagaaa gcgaacacaa ggacgataac ccggcacagc
    17221 ctacaaagaa gaaattaaag ctttcccaat caataagggt tgatataaat aagcttgatg
    17281 aacttatgaa tctgatggct gaacttgtaa tagccagaag cagaatagcc gaaacgctta
    17341 aaaaattcag cataaaagaa gttgatgaaa gtctgtcgca actttcaaga attacgctgg
    17401 atcttcagaa tgtagttatg aaagtcagaa tggtgcctgt ttcctttgtt tttaacagat
    17461 tcccaagact caccagagat attgccaagg agcttggaaa agagataaac tttattatgc
    17521 agggtgaaga gacagagctt gatagaactg ttgttgatga gataggagat cctttgatac
    17581 accttctcag aaactctctt gatcatggta tagagtcacc cgaggaaaga atagcaaaag
    17641 ggaaaccaaa aataggtaca ataaggcttt cagccagaca tgaaggcaac ggtgtaataa
    17701 tagagatcga agatgacggt aagggcatgg acaaagaaaa aatacttaaa aaagctttgg
    17761 aaaaaggact tgtagaccaa accaccgcgt cttcaatgaa agacgaagaa attcttgctc
    17821 tctctttcct tccgggtctt tctactaagg ataaggctac agaactttca ggaagaggtg
    17881 tggggatgga tgttgtaaaa tctacagttg aatctctcaa aggaactgtt actcttgaaa
    17941 gcgaaaaagg cagaggaaca attgtaagaa taagaatgcc tctgacactg gctataattg
    18001 aagcacttct tgtaacagtt aaagattatg tttatgccat acctatagct aatatagata
    18061 cgacgcagaa aattgccgac ggggaaatca aagttgtcca ggacagggaa gttttcatgc
    18121 taagaggaga agtaatacct gttataaggc ttagggagct ttttggctac agcaggactg
    18181 aaaatcttaa gcaagagaac atagttataa taaaagttgg aaataaaaag tatggagtca
    18241 ttgtggacaa actattaggt caggacgata tagtaataaa atctctcgga agcatgctct
    18301 cggatgtaaa ggaatttagc ggcggagcaa tactgggtga tggaagaatt gcccttattc
    18361 ttgatgtttc ttcattaatg taattaagag gtggttgaaa tgaaagatgt attgtctttt
    18421 cagatccata accaggagtt tgctgttgaa gttgatctca tcgagatagt aagcgatatg
    18481 gccgatatta ctcctatacc taattcacgc cagagcatta aaggtgtaat aaatctcaga
    18541 ggtcgtatag ttcccatagt tgacataaca gatatcctga gactcaatct gccttctgat
    18601 catgtgtacc gaaatatttt ggttctcaag gtggatgaag aggagatagg tattctggtt
    18661 gacttggtga ataatgtcct ttccatagat gagacaaaac ttgagaatct gcagtctagg
    18721 aatgagtttt atgctggtaa ggtcaacggt gtaattaaaa aggacaaccg gttgattgtt
    18781 tttcttgata taagggcagt tttaggtatg gaagaatgat aaaaattcgg gaggtgtttt
    18841 ataaatggga aaaagaattc ttatagttga cgatgccgct ttcatgagaa tgatacttaa
    18901 agatataatc acaaaagcgg gttatgaact tgccggagaa gcccagaatg gtcaggaggc
    18961 aatagacaag tataaagagc tcaagcctga ttttgtcacg atggatataa ctatgcctgt
    19021 tatggacggt attcaggcga taaagcagat aaagactatc gatcccaatt caagagtaat
    19081 agtctgcagc gctatgggtc agcaggccat ggttattgaa gctatccagt cgggtgcaag
    19141 ggactttata gtaaagccct ttcagcctaa cagagttatt gaagccatcc agaagttaga
    19201 gcagggatga ttcctacgga ctctacaaca tatattccaa cctccactgt tgctccgaca
    19261 gccagtgggg gaagttttat attagtatgg atattgacac ttgtactgtt tgtggttctt
    19321 ttatggctgc tctatatgtt tcttgcaaaa tataccaaag gaaccaagaa taatccgcag
    19381 ataagggtaa tgaaaaggtt ttacatcgat aggaactttt atgttggttt attaaagctt
    19441 tttgatgagt actatcttat tcttttcagt agcaactctt cggaacttct taaaaaactt
    19501 acttatgaag aagctttgga aataatcggc gaaaagcaga ctttttttaa aacttttgcc
    19561 ggtatattaa aaaaggggtc ggaagaaaat gagactaaga ctaagtagta agatcatact
    19621 tcttactttg atggttctgg catttagtac cagcatttca tccgctccgg ctgatcttgg
    19681 actgacactt ccgaacatag atataaaagt aggcagttct gagcagaatc ttacaccggc
    19741 aatcacaatt tttctcataa taactgttct ttcacttgcg ccgagttttt taatgcttat
    19801 gacctccttt acaagaattg cggtagtgct tagttttgta aaacaggcaa taggtgccag
    19861 gcaggctcct cctaaccagg ttgttgtggc cttagcactt tttctgacta tactgattat
    19921 gtttccagtt ttttctaatg tgtacaataa agccatagtt ccgtataatt ctggaacaat
    19981 aggatatgag aaggctgtgg agagaacttg gaatgagttc aagaccttta tgctccgtga
    20041 gatagtgaat cataaaaatc aggataatat atatatgctt gcacaaagca ccaatataga
    20101 aataaaagat attaacgata caccttttca ggttttgata ccggcatttg ccctcagtga
    20161 gcttgagata tcctttaaaa tgggaattct catatatctg ccttttataa ttgttgacat
    20221 ggtggtagca agcattcttt tatctatggg aatgatgatg attcctcctg ttatgatttc
    20281 tatgccattt aaaattttgc tgtttgtatt ggttaacgga tgggatcttc ttatagggag
    20341 tcttgtaaga agttttagcg gaggctgatg atgagcgaag aggtattcat agaagttttc
    20401 atagagggaa taagaatttt tttagtaaca ataagtccta ttttgcttgt cagtcttggg
    20461 gtaggtttaa tcataagtat attccagacg gtgacctcaa tacaagagca gactctcacc
    20521 ttcgcaccca agattatagt tacttttctt gcgcttgctc tcatgttcgg ctggataata
    20581 cagcagatag gtgattatgc ttatcgactt ttcacaacat acatgggaat gatataaatg
    20641 tatttttatg attatcagct atggatatac ttttttattc tcataaggat agtaggtgta
    20701 tcctttttta tacctttttt ttcaacaagt cttttgccga cacctgtcaa aattttcttg
    20761 gttatttttg taagctatat ctgtttgcct atgtcaacgc agacgatgcc attaaataca
    20821 gatgctctta caatagtctt ttatcttctt cttaacttta cggtgggaat aagtatcgga
    20881 ataataactc agatcatctt ttctgctgtg cagtttgccg gagatttttt tgggatacag
    20941 atgggctttg cgctggcgga agtcctcaac ccggtatacg aggaggaggt ttcgcttttt
    21001 tcagaactta cttttcttct tactactatg atttttttca tctttaaagg tcacatctgg
    21061 ctttatactc ttataattga gtcttttaag aaacttcccg tcgtattttc acttaatcaa
    21121 gatgttttca tggttctctc acagaaatta ggggatgtaa tgataatagg agtggagttc
    21181 gctttgccta taacggcatt tatgctttta accaacatat cccttggtat agcttcaaga
    21241 cttatgccac agctgaatgt ttttatgatt ggacttccta tgaacataat aatcggattt
    21301 cttatattta cggtggtgat atacacttgg gaagaaaatt ttaccggact tttctttcag
    21361 atttatactt ggcttcagag tacgataacc atgtttgcaa aatagatctt cagttttttg
    21421 ccgatccgtc caagacggaa gagcctactc agcgtaagct tgaaaaagca agagaagaag
    21481 gaaatgttcc tgtatcaaga gagtttacca caggtataac tttccttgcg cttgcaggca
    21541 ttctttcatt tttaggtgga ccgatagcca aagaacttga aaaacttttt tatgattatt
    21601 ttgatatgaa tacagatctt ttaaatccgt cattttttat ttactctctg ggcacacatt
    21661 ctaagcttta cgtatttatt gcactgtttt tcggaatggg gatggcggtg actctggggc
    21721 taggttttat tcagaccaga tttatgatag cgcctaaagc acttaagttt gatctttcaa
    21781 agataaatcc tataaaaggc ttcaagcgta ttttttccat gcggtcgctt tttgaacttt
    21841 ttaaggcaat tttgaaaata attataatag gctgggttgc ttattcaatt atttccggta
    21901 agatgaacga tataaccaat ctggccggat cggaaatagc tggatcagta aagttcattg
    21961 tcaatatgat acttgaactt atgtataaac tcggactttc ccttcttata ctttctcttg
    22021 cggattattg gtttcaaaaa tttcagtata ggcaagatct taagatgacc aagcaggaag
    22081 taaaggaaga aatgaaggat atagaaggaa atccagaaat aaaaagaaaa cagcgtgagt
    22141 ttatgaccaa gatagttttt tccaggatga tgcagcaagt tcctacggct gatgttgtag
    22201 ttaccaatcc aacacactat gcagttgcca taaagtatga aagcaaagga atgaatgctc
    22261 ctaaggtagt tgcaaagggc gttgatgaaa tggcgatgag aataagaaag gttgcttcag
    22321 aaaacggagt tccgatagta caaaagcctt cacttgccag ggagctttat gcgaaggtcg
    22381 agctagatga agaagttcca caggatctat acaaagctgt agcggaagtt cttgcttata
    22441 tttataaact ttcaaataag aatgtttagg aggaatggat gaattttata aaaaaccttg
    22501 atgtggtttt ttctgttctt atagtagtaa tggttttact tatggttctt cctataccta
    22561 catttttact ggatttttta cagctgttta acatagctct gtctataata atacttttgt
    22621 caacacttta tgtcaaaaga gctcttgaca tatcgtcttt tccgagcttg cttcttataa
    22681 cgacgctttt ccgtctggca ctgaacgttt cttcaacaag attaatactt cttaagggac
    22741 caaaatttga cggaaaggta ataagagcgt tcggcgattt tgtggttgga ggtaattaca
    22801 tagttggtat agtaatattc cttatccttg taataataca gtttatagtt attacaaaag
    22861 gttctgaaag aatatctgaa gtagctgcaa gatttacgct tgatgctatg ccgggtaagc
    22921 agatgagtat cgatgcagat ctatcttcgg gacttataac agaggatgag gcaaagacca
    22981 gaagagaaga tataagaagg gaagctgatt tctacggggc tatggatgga gctagcaagt
    23041 ttgtaagggg agattccatt gccggtttga taattacgat tgtaaatata ctggggggca
    23101 tcctaatagg tatacttcag cagggaataa gcatacagga ggcagccgga ctctatactc
    23161 ttctgacagt gggagatgga cttgttgcac agataccgtc gcttttgata tccacagcgt
    23221 caggtatggt tgtttcaaga gcagcttcca aggagagttt tggttccaac ataataaaag
    23281 aacttacttc agaaaaaaag attctttcaa taactggttt tatgcttata gctatcggcg
    23341 ttttcacccc tcttcctaca gcaccttctt tggttatagg tggagttatg ctttttgtag
    23401 cttactctgc aacggcaggt cagaagaaga aacttgctta tcagacagct ggcggaagca
    23461 tgcaatctaa aaacattctt tcacagcaaa atgaagcgag tggtggccaa agtgtgccgt
    23521 ccaaatccgt aaaatcagca ccaccgctga cttctgccga tgaagtttca gaagtgatac
    23581 agagcgatac aatcgaagtt gatataggct acggtcttat tccgctggcc gatccgacac
    23641 agggtggaga tctacttgac agggttacaa tggtacgaaa gcagatagct tatgagcttg
    23701 gtgtggttat agcgccggta agaataagag atagtgttct tcttagctca aacgagtaca
    23761 taataaaact cagaggagtt gaaattggaa gatatgagct tattccaaac agattattgg
    23821 caataaattc aggaatggct aacatggctc ttgcaggggt tcagtcaaaa gaacctgctt
    23881 ttggacttgc ggcgtattgg atagatgaag gtcttaagga tgatgccata agcatgggat
    23941 ataccgttgt cgatgcaccg agtgtttttg ctacacacct ttcagaacta ataaagcgtt
    24001 atgcccatga aatagttggc atgaaagagc ttgagatgct tattgacggt ctaagagtta
    24061 aaaatgcgac tcttgtcgat accctcatac cagcacttct taaacttcat gagcttagaa
    24121 aggtcataca ggatcttctg tacgaaagga tatccataag gaacatgccc atgatatttg
    24181 aacagcttat agaaacatca gataaaagcg gaaaggatca ggaaaaactt gtggaggatg
    24241 tgagagttgc acttggcaga cagatatgcg aatcacttaa atcttcagat gggcagtttc
    24301 atgtcatagc gctcgatgct tccatagaaa gaaaaatttt ggacagtttt accgacggag
    24361 aaaacggacg gctgttgatt cttgagcctg aatacgccaa tcttctcata gaaagggtgt
    24421 ccaagggact cgaagagata atgatgaaag gatacaatcc ggttctgata tgttccaagt
    24481 ctatacggta tcagcttgca aagctaatac tcaggttcat acagaacata aatatagttg
    24541 cctatgaaga agtaccttct gacgtcgctc tcagcgttga agggatagta aatttctaaa
    24601 ggggaggtaa cttatgaagg taaaaaagta tatagtgaat gatcttgcgg aggctatgac
    24661 aaaaatcaag gatgatttcg gtgatgacgc atatatatta gaaacgaaaa agataaaaaa
    24721 aggtggattc ataggatttg gaggtaaaaa gtttctcgaa gttacggtac tgagtgaaaa
    24781 agaagaagaa aaagccagaa accaggtgtc caacggaaat aaagaaaaag cagatacctt
    24841 tgaaagcgat ctttacgata tgaagagtgt gataagtaat aacaaacgtc ttgaaaaaag
    24901 aataaaacag gaaagcatgg cggctcagcg tgatattcca caggaatatg aggttcagaa
    24961 tacggaaaaa gagtataaca aggattttat tgaacttata aaacagcagc gtgagctttc
    25021 taaaagcatg gacaattcag ctaaggattt ttatgaacag cagaatgtta cggctgaacg
    25081 tccggtaaag aatataaagt acgatacata cacaaggaat caggttatac cagaaacatt
    25141 tgctggtgcc gcagctgaaa aagcttctca agttaacaac gattctgtca gtgagataaa
    25201 aaaaatgttg agcgagctta ataaaaaagt atccggtgaa tctgaatata taaatgattt
    25261 cagacaggca cttttgaaaa gcgatttttc agatgaaatc attgatgaat tcttatctca
    25321 gcagactgga aacataaaag aagactggaa gagtaatgtt caactcagag aagcatttac
    25381 aaagtatttg gcagcaataa taaaaaaaga taagcagcag tttaaagata agataatcct
    25441 tgtcggaccg acaggtgtgg ggaaaacgac aacgctggct aagatatcgg caatacttaa
    25501 aaagcagggg aaaaagatag ctatcgttac ggttgatacg tacaggatag cagcggcaga
    25561 tcagctcaag atctatgcgg atataatggg agtgcctgct tttatatgct atactcctgg
    25621 agaccttagg agcactcttc agactcttgt gggttatgat gccgtgctta tagacactgc
    25681 agggcgcagt
//
LOCUS       DAOSHR010000006        25471 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_1528_length_25471_cov_5.627321, whole genome shotgun sequence.
ACCESSION   DAOSHR010000006 DAOSHR010000000
VERSION     DAOSHR010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 25471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25471
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1528_length_25471_cov_5.627321"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..856)
                     /gene="metF"
                     /locus_tag="PLU28_00655"
     CDS             complement(<1..856)
                     /gene="metF"
                     /locus_tag="PLU28_00655"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HOG33603.1"
                     /translation="MKISEKFLGGPLISFEIFPPQKDSPLSSIFKTIDELADLKPDYI
                     SVTYGATGGQSIRTAEIASIIKNKYGIDPLAHITAVRNSKDDIRKEVERMYDNNIENI
                     LALRGDNVPAEAQANDFKYAQELIQFIRDEYKDKFCLAGACYPEGHVESRNKSLDLYY
                     LEKKVSSGVDFLITQLFFDNAFYYDFASKLKNRSIVIPVQAGIMPVTNKNQIERITSM
                     CGAFIPEKFIKVMNRYQGDKQALTDAGVAYATDQIVDLLSSGVDGIHLYVMNKPQVAR
                     KITQNIKTV"
     gene            complement(868..1572)
                     /locus_tag="PLU28_00660"
     CDS             complement(868..1572)
                     /locus_tag="PLU28_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0280 family protein"
                     /protein_id="HOG33604.1"
                     /translation="MFEERSYRRIMNPYGLETFDVKVYESDLRVFCDRDVNESIYKNV
                     CRYRRIIEEYGSTNSIFLTTHEPLLYDINAHDIIRHMQQASSSAGVGPMAGVAGAVAY
                     YASLGIQCQELIIENGGDIYLKSVQDRTVKIHTANKYFGDKIKILVQSGEWGVCTSSG
                     TLGHSFSYGKADAATIIAHDPVLADCVATAACNMVKIEEDIQRAIEFSMSIKGVDGAV
                     VIVGSKIGMKGNIKLV"
     gene            complement(1565..1753)
                     /locus_tag="PLU28_00665"
     CDS             complement(1565..1753)
                     /locus_tag="PLU28_00665"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOG33605.1"
                     /translation="MKITIDKSKCVDCGVCIAACSYGALYMDNEFVLQYDYEKCQRCM
                     MCLKACPLRIIGAVGQDV"
     gene            complement(1734..2909)
                     /locus_tag="PLU28_00670"
     CDS             complement(1734..2909)
                     /locus_tag="PLU28_00670"
                     /inference="COORDINATES: protein motif:HMM:NF013958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine biosynthesis protein"
                     /protein_id="HOG33606.1"
                     /translation="MSKNYEEINKKIQRGEAVVVTAQEIIDIVQEKGVKETAEYVDVV
                     TTATFGPMCSSGAFLNFGHSDPPIRMTKTYLNGVESYSGLAAVDAYLGATQNSEDKGM
                     EYGGAHVIRDLIERKKIHLKAYSYGTDCYPEKEIDTFIDIDSINEAYLYNPRNVYQNY
                     NAAVNSSEKLLYTYMGILHPFMGNVTYSGAGELSPLINDPYYRTIGVGTRIFIGGSIG
                     YVSWSGTQFKSSVCRGGNGVPIGPGGTLALTGNLKEMDSRFVQPAVFEKYGVSMFMGI
                     GIPIPVLDEDMLRYLCVSNRDIYTNIIDYGCPSRSRPVIKKVSYAELMSGKVEINGHN
                     IRSSPLSSMRKAREIAQILKKMVASGRFMLTQPVKNFDKDMALKSLERNGKNEDNDR"
     gene            complement(2914..4152)
                     /locus_tag="PLU28_00675"
     CDS             complement(2914..4152)
                     /locus_tag="PLU28_00675"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF005154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOG33607.1"
                     /translation="MSIIVQKYGGSSVADENAIKKVAERVIKFHDTGNKVVVVVSAQS
                     GVTDTFLEKVFKLSCMPNERDVDMLLSSGEQIASSLLSVAVNEKGRKAVALLGYQCGI
                     HTDNVYSKARIKKIDAEKIIQHLDKGEIVIIAGFQGVNDCNEITTLGRGGSDITAVAI
                     GAALNAEYVEICKDVDGIFTADPRICKDAVLLDRVTYDEMLEMAASGTKALNSRSVEI
                     GMKYGLKIYVRSSFKAQDQNNPGTLISMEVNTMEEPVIRAVSHSLKETKVVITQKNAS
                     PAAITEVFDIVNELNINIDIISDIALDGYTCVAFTISKDEAYKTDELYLRLKSRLSNC
                     EIKVEKNKCKLSVIGTGIKSTPGIASKVMKVLLKNGIGIEMISCSEIKISCIVDEKYA
                     EKGVNLLHEGFIAINNAMRG"
     regulatory      complement(4231..4332)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            4702..6030
                     /gene="glnA"
                     /locus_tag="PLU28_00680"
     CDS             4702..6030
                     /gene="glnA"
                     /locus_tag="PLU28_00680"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HOG33608.1"
                     /translation="MAKKEEVLKKISEEGVKFIRLQVTDINGTLKNVEIPADELENSL
                     KNGTMFDGSSIEGFVRIEESDMILKPDIDTFCILPWTMEKEKVARFICDVYTPDGKPF
                     PGDPRRILRNVTEELAKHGYTGFAGPEPEFFLLPRDEATRKPKLAFMDEGDYFDLLPI
                     DLGEETRKQIVVSLEEMGLNVEASHHEAAPSQHEIDFTYADILKTADNIQTFKLVVKT
                     IALLRGLHATFMPKPFFGINGSGMHVHMSLFSNASNIFFSENSPYQISNELRWFVGGI
                     FNHIDAITAIANPSVNSYKRLVPGYEAPVNIAWSASNRTALVRVPASRGKGTRVEFRS
                     PDATSNPYLLLAVVFACGLDGIINKCEPHESVNENIYLMTEERKKELNIRQLPQNLQE
                     SIEALKKDSFIKGVMGEHVFSKFIQLKEQEHHSFSCSVTDWEIKKYLHLI"
     gene            complement(6058..6522)
                     /locus_tag="PLU28_00685"
     CDS             complement(6058..6522)
                     /locus_tag="PLU28_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOG33609.1"
                     /translation="MTEVKIVTFNLGKEKYGLDIMKIDAVADYEEVTVLPQAADYVEG
                     IINFRKREVLPLINLRKKFKMKDFADKTKSKVIVIKMENKKIGAMVDEVKEVMSIPAN
                     VLEETPDIGGLKNSRFISGVARLNNEMIIILDIDKLLTEEEKIELSGIVENV"
     gene            complement(6616..7059)
                     /locus_tag="PLU28_00690"
     CDS             complement(6616..7059)
                     /locus_tag="PLU28_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG33610.1"
                     /translation="MLYSLIFIALNAAGGWFFTVEKLYFWISLVPLAAILLITLKHKK
                     FDKLTTLVLFLSAVLTLIGSIRDINSGLFFVLYNTAAFISSIGFLSGKKIFYLFSWII
                     NGASIGFLVSQIRGIPLGVLFGILIFFTGFRYFTNFKMPGKLTEK"
     gene            complement(7069..8082)
                     /locus_tag="PLU28_00695"
     CDS             complement(7069..8082)
                     /locus_tag="PLU28_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOG33611.1"
                     /translation="MKIDFSYEKKPIDFISGYIYKGSGYKCNYIRFPTLYKTPARGTE
                     TVELYNFQAREKSMASVLILHGLGSSNVKFLLWMGNHLASSGIDASILILPGNYTRVD
                     DNSTSGRGYVYPDINVMYNFWQHAVVDVMSTIDFLKEKEMWAENNCVLGYCLGGMVGT
                     IVSSMRDDIGELILMTAGGHLPKILYESPATRSSRYIQQKKNLSFIEEKERIYRIYEK
                     QMPLVRNMCLTQLIESEEIHPIFKVDPISYAHLVDRSKVTFFEAIFDRTIPILGRKLL
                     WEELKKPKRYLLPIDHVSWLFFEYFLSVYIMNKLNIKGIKIKARKNREDKERTIDDVL
                     LGK"
     gene            complement(8072..9082)
                     /locus_tag="PLU28_00700"
     CDS             complement(8072..9082)
                     /locus_tag="PLU28_00700"
                     /inference="COORDINATES: protein motif:HMM:NF015892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOG33612.1"
                     /translation="MEKLKIISSNARKYFLVKFKFVIARIILIGVFILSIIFNGIILI
                     YSFITLVVLFIIKVLTSRLPLQTDEYDGPYTYVYKQEKKDKLKLDIWYPLETRKKYPV
                     VFFAHGGGWIAGFRNQPNNVSWCRYLASRGFAAVSIDYRYGFKSTMFDILSDYDDGLK
                     YIREHSQRLGFDDENIFLMGLSAGGHMSLLYAAYNTFVRDFNAMKGVRAIVAYYAPSD
                     LQEVFSVTDSVFAKIATRTTLKGTPKKLPNEYAMYSPIKWISERMVPVLLVHGKEDKT
                     VPVKSSYDMYESLLNYKVDSELIIHRHGGHTFEFELKDIDTLKIIRDTVNFMKEHIKN
                     ED"
     gene            9206..10087
                     /locus_tag="PLU28_00705"
     CDS             9206..10087
                     /locus_tag="PLU28_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG33613.1"
                     /translation="MDRLLIYYGFPSSVNHADSILRATEYFSSYDTVVFAGSRKNFKS
                     IEFAEHIDHKNSEAIIKNLKNNTFGYVACGNRDGVDSCWTNSEIKDLCIRWKNMGIKG
                     IFLDELGYDFNNFKSRKTDILNIVRNLSLSVFTNCWSPEELFDDSPDLFTKNDYYLLE
                     SLFFFANGEITEFQSFKDSFQRLSTAQQYRNALGFKLACANSFGKDYNLIGAGFMDFT
                     ISAADFFSLDALSITKGNYHSNDVDLIPFDIKESGYSKKFKIKFFLKTVKRNDQHDKY
                     IKFRKDKFSQLPVEYRL"
     gene            complement(10137..11414)
                     /locus_tag="PLU28_00710"
     CDS             complement(10137..11414)
                     /locus_tag="PLU28_00710"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HOG33614.1"
                     /translation="MSSLYESAVRQFNKAAKIMELPDDYKEVLTKPKRELTVNFPVKM
                     DNGKIQVFTGHRVQHNIARGPAKGGVRYSLDVNLDEVKALAFWMTWKCAVADIPYGGG
                     KGGVCVDPKKLSEGELERLSRRYFSEIQVIVGDEKDIPAPDMGTDGRVMAWYMDTYSM
                     NIGQPDLAVVTGKPLEIGGSQGRTEATGRGVMISVGEAVEYLRSKGKITKKNNELTIA
                     VQGFGNVGSYAAILIAEELGLKVVGISDASTGLYKEDGFTGKEMREMMEKILARRVLL
                     DAVNPGYKKITNEDLLTLDVDILCPCALQEVITERNAASVKAKLIVEGANGPITPDGD
                     EILLSKGIFIVPDFLANSGGVTVSYFEWVQGMQWHFWGIDEVRKALKQKMTSAFADTI
                     KQMEKYDTDMRTAAYIVAVSRVYNTTKLRGIYP"
     gene            complement(11608..12075)
                     /locus_tag="PLU28_00715"
     CDS             complement(11608..12075)
                     /locus_tag="PLU28_00715"
                     /inference="COORDINATES: protein motif:HMM:NF016160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HOG33615.1"
                     /translation="MRELMNVKEVKDGYVYMLTQRKVTCESCAMSGACSIMGSPDTKV
                     KAKNIDKLDLKEGDYVVVELPNVSVTKLSFLVYGIPLIVFIVFTVLFYYLNFGDYVSF
                     LLGLAATALSFVFMGYLDRHKFQDKYLPVILEKYELPVAVSANKSIDDGLKQQ"
     gene            complement(12222..12797)
                     /locus_tag="PLU28_00720"
     CDS             complement(12222..12797)
                     /locus_tag="PLU28_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOG33616.1"
                     /translation="MRGGVDLKEEKFIKELKKKDSKAFEKLYSDYAAMVFGILRNFVR
                     PSEIEDAIQEVFLRVFRGIDKFRGSSKLSTWIYRIAVNVGKDYVKKYSEDKIQTIDLQ
                     NDVNEKYLKSSVSSESQDDALQGVNYSSIIKTMEKLSPGDRLIIQMRDLDRLSYKEIS
                     TILEIPLGSVKSRLYYARKRLGKLIEQEKII"
     gene            12911..13216
                     /locus_tag="PLU28_00725"
     CDS             12911..13216
                     /locus_tag="PLU28_00725"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOG33617.1"
                     /translation="MAFKITYDSTRIFVSDLPLIDVCVAKDLLNSIQEIPEKSVKEYF
                     FDFSNLTYLDSAGLGALLALHRKASKTNGKIIIKGAKDNIKELFIITKLDSIFELQN"
     gene            13945..15675
                     /gene="thrS"
                     /locus_tag="PLU28_00730"
     CDS             13945..15675
                     /gene="thrS"
                     /locus_tag="PLU28_00730"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOG33618.1"
                     /translation="MIITQEKRKTLWHLSLHLLAIAVKKLYPQAKLGNGQVTDEGFFY
                     DFDNLTIKTSDLTLIQDEMKKLIDEGINCENILLPVQQALDFFCNEPYKLQILERMEK
                     TEKISLCSIGDFYDICSQNHSDKCNNLIAVKLLSIGGAYWMGNPKNQMLTRISGIAFE
                     NQEALEEFIAYSELLKKHDHRVIGKQLELFSFHPQAPGSVFWHENGLILRNLIVSYWR
                     KVHRKNGFREISTPILLDSSLWKQSGHWELYKESMYTTQIEEKEYAIKPMSCPGSILY
                     YLEKNRSYRDLPLKVAELGLVHRNELSGTLHGLMRVRQFTQDDAHIFVCPNQLSEQIQ
                     QVIKMSVDMIGEFGFKNFSFAVSVRSPEKMAKYLGDDADWDKAEAALKSSLEALNYTY
                     CISEGEAKFYGPSIDIMINDCLGRKWQCSTLQLDFNLPQRFELEYTDAHGEKQTPFML
                     HRTLLGSLERFIGILLEHTKGQLPLWLSPVQVAVMPISSKYSEYGCTIFRMLEEKAVR
                     AVLIDSEESLSKRIRQTVLLKIPYIVVLGEREVSNSVISVRKADEDESCVYNLQEFID
                     YIKKVERYRI"
     gene            complement(15710..18064)
                     /locus_tag="PLU28_00735"
     CDS             complement(15710..18064)
                     /locus_tag="PLU28_00735"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HOG33619.1"
                     /translation="MTERVEKLRKESLEVQARISMERAVLMTQAYEKYEGRVPVPVLR
                     ALAFKHIMENKHIDIYDGELIVGERGPSPAHTPTFPELCCHSSEDFEIIDKREKIFFK
                     TDEQSKKIQREKIIPFWQERALRGRILAMMSQEWKDCYSAGMFTEFMEQRGPGHTVAD
                     DKIYRKGFLDFKNEIKKSIELLDWEKDFNALNKYDELTAMDICCDAIITYARRYSALA
                     QKKSEEEKNEKRKEELQEISRICRKVPEYKPDTVHEALQMYWFVHIGVISEMNNWDSF
                     NPGRLDQHLFPFYSKQLMEGTIDREKAKELLECFWVKFNNQPAPPKVGVTLKESSTYT
                     DFANINIGGLKSDGSDGVNELSYLLLEVVDEMRLLQPSSNVQISKKSPDAFLLKALDV
                     IKKGWGQPSVFNADAVVQEMILAGKSIEDARCGGTSGCVETGAFGKEAYILTGYFNLP
                     KVLEITLNNGYDTNTHKQIGLNIKIEEAGSYTEFFEIYKKQLNHFIDIKLKGNMIIEK
                     MYSEYIPVPFLSVVIDDCIKKGSDYNSGGARYNTSYIQGVGIGSITDSLSSLKQHVFE
                     EKNFSVMQVKEILAKNFSGYDDAYKLFKSYTHKYGNDDDYADSIMKEVFEAFYSCVTD
                     RNNVKGGKYRIDMLPTTCHIYFGSVCGASADGRKAFEPLSEGISPARDADQNGPTAVI
                     KSASKMDHIRTGGTLLNQKFLPSVFEDQENIVKIKDLIRVYFKRDGHHIQFNVVSRDT
                     LLKAQKNPQQYSNLIVRVAGYSDYFNNLSPELQNEIINRTEQQI"
     gene            complement(18066..18947)
                     /locus_tag="PLU28_00740"
     CDS             complement(18066..18947)
                     /locus_tag="PLU28_00740"
                     /inference="COORDINATES: protein motif:HMM:TIGR02494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HOG33620.1"
                     /translation="MVFKIQRFSLHDGPGIRTVVFFKGCPLRCFWCHNPEGQSFEKDL
                     MFYASRCKMCGKCIEACPKEAIYFYQGRITTDLSKCIKCGRCVMICPNQAREVIGTEM
                     DVDEITDVVLKDMDFYEQSGGGVTISGGEPLANTQSLELLRRLKRENIHTAVETSGYC
                     QWEYMKEAARHTDLFLYDLKVADNQKHIEYTGCSVEPIVDNLKKLSSLESEILVRIPL
                     INGVNTQFKELEQMDNILKYCEIKKIQLLSYHDYAKNKYDALARNYQADNQNKLPPGT
                     FAQVQEFFRKRNYEVGG"
     gene            complement(18937..19521)
                     /locus_tag="PLU28_00745"
     CDS             complement(18937..19521)
                     /locus_tag="PLU28_00745"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HOG33621.1"
                     /translation="MKNILTLGKKIKEYRLKSRMTLKDLSEKTGLSAGFLSQLERGMS
                     TVAIDSLMNIAKALDTNIKEFFSDEDIMAGEIVRSYEREVLKIHENGYIHYLLSANQD
                     TKNILPRVVEILPQKNTEKIQKYSHEGEEFVFVLEGILTLYLAGEIYDLYPGDSAHYI
                     SRKPHNWQNRTNKTVKILSVNSPNFLKEGRSYGF"
     gene            20100..20777
                     /locus_tag="PLU28_00750"
     CDS             20100..20777
                     /locus_tag="PLU28_00750"
                     /inference="COORDINATES: protein motif:HMM:NF020916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HOG33622.1"
                     /translation="MIPRSELKHFINFSDYLAIRNRLKFIASYDTNADKNGEYTVRSL
                     YFENYNNKVLAEKALGTDHREKYRIRLYNNNHKLIKLEKKIKKDRFTTKFSKEITKSQ
                     CTDLINGSYDFLSHSNIPLFNDFFSKIKNDMLRPRTIIEYKRQAFFYPYGNVRITFDM
                     DIKTALGSVDILNPELVLVSSLERNQIILEVKYDRFLPQIISDCIQLNERRQISASKY
                     ALGRQYL"
     gene            20807..21493
                     /locus_tag="PLU28_00755"
     CDS             20807..21493
                     /locus_tag="PLU28_00755"
                     /inference="COORDINATES: protein motif:HMM:NF027640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HOG33623.1"
                     /translation="MNSTVNFSDIFKSNFLEKVSSFSFVDMLVALALAFAVGLFIMAV
                     YKKTFRGVLYSQSFCVSLMALTLITTLIILAVTSNIILSLGMVGALSIVRFRSAIKEP
                     IDIVFLFWSISAGIILGAGLIILGVFGSILIGFILLVFVNKKSIDNPYILILNCENDS
                     TEKQALEFVSNNVKKHVVKSKTVSRENGIETTVEIRLKSSSTEFVNSLGNMSGIKNVV
                     MVSYNGEYMA"
     gene            21523..23475
                     /locus_tag="PLU28_00760"
     CDS             21523..23475
                     /locus_tag="PLU28_00760"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HOG33624.1"
                     /translation="MMKEKAITVVTVLSVCCAVLLIIWLVFFCDLSGLKKSQTPEYMV
                     KIFDKNQIIDINIFIDKEKWTEMLENATAQEYYTCNVSVNSKKYYNVGIRTKGNASLS
                     MIRSSSQPQRYSFKLDFGEYVKDQTLFGLQKIALNNMIGDASYMKEYMSYEMFSALGV
                     PVPGYAYASISVNGEPWGFYIANEDIDDTFVERYYDDLSKVNVYKPETMEMNGEDGQQ
                     PGGFGRNGSTGTNLVYTDDNLSSYSGIFDYTLFETTGDDDKMKVVEMMKSLSTLSDIE
                     KHINTEEVLKYFAVNTFLVNLDSYAGSMKHNYYLLENDGLFEILPWDLHLSFGAFQMQ
                     QTSRVINFPIDAPVTDSMQNSPLISKLLEVDKYKTMYHSYLQKLIQEFIDSGKYYERI
                     KQVSSMIDSYVKNDSMAFYTYEEYQSALENMIIYGKDRTLSIKAQLEGSQPSTEYGNI
                     QSSLNLTALGTMGGGKNVGDRMMNTNQQSQDFFDTADASGTQMRQFQAPGEQNGAQSM
                     PNQFGQPPADMQSMEPPDNQGQENMQPGNMPEPPEGFSSRQPPEFSEKRGFGPRPNQD
                     QPGNFQFQNSTQASSTTAISSETALNSTPILPNGQNMRKNEINRNSPFRQADHTLEYA
                     ALGVSFAAIFIGLIAVFRFKKRKYSS"
     gene            23654..>25471
                     /locus_tag="PLU28_00765"
     CDS             23654..>25471
                     /locus_tag="PLU28_00765"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOG33625.1"
                     /translation="MKSIKFKIALISIIISSLAYFLILAITYSTVTQSIVSQNEQFTR
                     KILSLKSQQLENYLDGIILHYSGYIKNSWLIKDSLVLSYWDMIKSELTTKILEKEEDF
                     YDIILISESGKAFSSLEGEKEDFSDEGLQIFKTCPEDTYIRVETKGSDTFLNAYFRVY
                     DETLKVIGFSILKIKPDFIKLSVGDINIVGVVSSSLITDDGSFFSTTADITQDRILVS
                     ADIASENGWKIGAYIDKKILNAELDRIIRLLSITVVLITVISTFMYILTGAFLLNPLK
                     DLEKGLEIIKQGNLKKDLRVRTKDEIYRISCKINEVTEKLRDVMAAILDTTNELSSSS
                     NDFKIISHTLKDSSDRLHADMTQTHSKTDRFKLALLDFEKYMQHLESDMNENHSYSEK
                     IEVFAGDIKKESYYGLSKINQCLDFSEKTIQRIELSDRYVGVLAKHSESIEEIVHAIE
                     KITSQTNMLAVNASIQAAKAGENGKSFGVVASEIRKLASESKTATEKIYIVLKQIKDD
                     GDKAKNLSAECLEDIRTNNSNIMGVSSEFSSIIEKIVELSVMLDKMKKITQDNFSETS
                     NIIVNLKNLSKALVVIQSEITEVAETCSNQKAISKKIKDH"
BASE COUNT         7781 a         5145 c         4272 g         8273 t
ORIGIN      
        1 tgacagtttt tatgttctgg gttatttttc ttgctacctg aggtttattc ataacataaa
       61 gatgaatccc gtcaacacct gaagagagca aatctactat ctgatctgta gcatatgcaa
      121 ctccggcgtc ggtaagcgct tgtttgtcac cctgatacct gttcattact tttatgaact
      181 tttctggaat gaaagctcca cacattgagg ttattctttc gatctggttt ttatttgtta
      241 ccggcattat tccagcttga acaggtataa cgatacttct gttttttaac tttgaagcaa
      301 aatcataata aaaagcatta tcaaaaaaaa gctgagtaat caaaaagtca actccgctgg
      361 ataccttttt ttcaaggtag taaagatcca acgacttatt ccttgactca acatgtcctt
      421 caggataaca tgccccggcc aggcaaaact tatccttata ctcatccctt ataaactgaa
      481 tgagctcctg agcatattta aaatcattag cttgagcctc tgccggtaca ttatctccgc
      541 gaagtgcaag aatattctct atattattat catacattct ttcaacttct tttcttatat
      601 cgtccttact gtttcttacc gctgttatat gcgcaagcgg atctataccg tatttattct
      661 ttattattga ggctatctct gcggtgcgga tgctctgccc tcccgttgca ccgtacgtaa
      721 cgcttatata atcaggctta agatcggcaa gctcatcgat agtcttaaaa atactgctta
      781 acggactgtc tttctgaggc gggaatattt caaatgagat caaaggccct cctaaaaatt
      841 tttccgatat tttcatgctt ttccctccta aaccagttta atatttcctt tcatgccgat
      901 cttactgcca acgataacca ctgctccatc cacacctttt atactcatag aaaattctat
      961 ggccctttga atatcctctt cgatcttaac catattacat gcagcggtcg caacacaatc
     1021 cgcaagcaca ggatcatgag ctattatcgt cgccgcatcg gctttgccat agctgaaaga
     1081 atgtccaaga gttccggatg aggtacacac accccactcc cctgactgca caagtatttt
     1141 tatcttatcc ccaaagtact tattggcagt atgaatcttg actgttctgt cctggactga
     1201 ctttaagtat atatccccgc cgttttcaat tataagctct tgacattgaa ttcctaaaga
     1261 ggcatagtat gcaactgccc ccgccactcc tgccattggt cctacaccag cacttgatga
     1321 tgcctgctgc atatgccgga ttatatcatg agcatttata tcgtataaca atggttcatg
     1381 tgtagttaaa aaaatagaat ttgtcgatcc atactcttca attattctcc tgtacctaca
     1441 aacattttta tatatacttt catttacatc tctatcgcag aagactctta gatcactttc
     1501 ataaactttt acatcaaatg tttcaagtcc gtatggattc attattctcc tgtaacttct
     1561 ttcttcaaac atcttgacca actgctccta taattctcag aggacaagcc ttgaggcaca
     1621 tcatacacct ttggcacttt tcatagtcat actgcagtac aaactcgtta tccatatata
     1681 gtgcaccata actgcacgcc gctatacata ccccacagtc aacacatttt gatttatcta
     1741 tcgttatctt catttttacc attcctttca agggatttta gcgccatatc cttatcaaaa
     1801 ttttttacgg gttgagttag cataaatcta cctgatgcca ccatcttctt aagaatttgg
     1861 gcaatctcac gtgcctttct catgcttgac aacggcgatg accttatatt gtgaccgttg
     1921 atttcaacct taccggacat tagctcggca taacttactt tctttattac cggtctgctt
     1981 cttgatggac atccataatc aatgatattt gtatagatat cccggttaga aacgcaaaga
     2041 tatctgagca tatcttcatc aagaacagga ataggtattc ctattcccat aaacatgctt
     2101 acgccgtact tttcaaaaac agccggttga acgaacctgc tgtccatttc tttcagattt
     2161 ccggtaaggg caagtgtacc tcccggaccg atgggtacac catttccgcc tctgcaaacc
     2221 gatgatttga actgcgttcc tgaccaggat acataaccga tgcttccccc tataaatatc
     2281 ctggttccta ctcctattgt cctgtaatat ggatcgttta tcagaggact aagctcaccc
     2341 gctccgctgt aggtgacatt ccccataaaa gggtgaagaa tccccatgta agtatacaaa
     2401 agtttctctg aactgtttac cgctgcatta tagttttgat atacatttct gggattgtat
     2461 aaatacgctt catttatact gtcaatgtca ataaatgtat ctatttcttt ttcaggataa
     2521 cagtctgtgc catacgaata tgccttaaga tgtatcttct tcctttctat cagatctctt
     2581 ataacatgag cccctccgta ctccatcccc ttgtcttctg agttctgagt agcgcccaga
     2641 taagcatcca cagcagcaag accagaataa gactctactc cgttcagata agtttttgtc
     2701 attcgtatgg gaggatcaga atgtccaaag ttaagaaaag ccccactcga acacattggc
     2761 ccaaaagttg ccgttgttac tacatcgaca tactctgcgg tttcttttac gcctttttcc
     2821 tgtacgatgt ctattatctc ttgagctgtt acaaccacag cttcccctct ttgaatcttt
     2881 ttatttatct cttcatagtt tttactcata agctcacccc ctcatagcgt tatttatcgc
     2941 tatgaaaccc tcatgtagaa gatttacccc tttttcagca tacttttcat caacgatgca
     3001 cgatattttt atctccgagc agcttatcat ctctatgcct atgccatttt tcaaaagaac
     3061 tttcattacc tttgaagcaa ttcctggagt tgattttata cctgttccta taacagaaag
     3121 cttgcacttg tttttttcta ctttgatctc acaattgcta agcctgcttt taagtcttaa
     3181 atagagctcg tctgttttat atgcctcatc tttgcttatt gtaaaagcaa cacaggtata
     3241 tccatccaga gctatatcag agattatgtc aatatttata ttcagctcgt ttacaatatc
     3301 aaaaacctcg gttatagcag ccggagaagc gtttttctga gttatcacta ccttagtttc
     3361 ctttaaagaa tgcgaaactg cccttattac aggttcttcc atagtattaa cctccatact
     3421 tataagtgtt ccgggattat tttgatcctg agctttaaac gatgatctga cataaatttt
     3481 caaaccatat ttcattccta tctcaacact tcttgaatta agcgctttcg ttccggatgc
     3541 agccatttca agcatttcat catatgtaac tcgatccaaa agtacagcat ccttgcagat
     3601 tctcggatca gccgtaaaaa tcccgtccac atccttgcat atctcaacat attcagcatt
     3661 cagcgcggct cctatagcaa cggccgtaat atctgaacct ccacggccaa gagtggtaat
     3721 ctcattacag tcattaactc cttgaaatcc tgctataata acaatttctc ctttatcaag
     3781 atgctgtatt attttctcgg catcaatttt ttttattctg gctttactat atacattatc
     3841 tgtatgaatt ccacactgat agcctaaaag cgccacagcc ttcctgcctt tttcatttac
     3901 ggcaacagat agaagagaag aagctatctg ttcgcctgaa gataaaagca tatccacatc
     3961 tctttcattg ggcatgcagg aaagtttaaa taccttttcc agaaacgtat cagtaactcc
     4021 cgattgtgcg gatacaacta ctacaacctt attaccggta tcatggaatt ttataactct
     4081 ttcagcaact ttttttatgg cattttcatc ggcgactgac gatccgccgt acttttgaac
     4141 aatgatactc ataatctttc ctcccgtttt gattaatccg aaagaactat aaaaaaatac
     4201 cctttccttt atgaaagagt agtttgatat atcttatctt tcatttcgga tgaaatgctg
     4261 gatttagcac cacgctgttc gcaggttgct gaagggtcaa caggccagtc cctcgccttc
     4321 tcttgataag ttttatatta aattttatta atactatcat acacttactt ctttgtcaaa
     4381 tccgcaggcg tcataaaaaa gtttcttatg gctctggtat ttatgtaaaa aactttgtaa
     4441 tgctctctaa tattgaactt aatttttttt gttttttttc ttttttttat gctccgcgct
     4501 aaagtacgct ttataaacta tattgagggc tttttatatt tttatttaaa atttgacttc
     4561 atagctcttt tgagatataa tttcgttatt gtttttgttt aatcctattt aattggcttt
     4621 ttctccgcta tactccataa atctctattt tattaaaaaa tctttttgtt tcatattcta
     4681 ttctttaagg gaggagttcc catggccaag aaagaagaag tattaaagaa aatatcggaa
     4741 gaaggagtaa agtttatacg ccttcaagta accgatatca acggtaccct gaaaaatgtt
     4801 gagattcctg cagacgagct ggaaaactca ctgaaaaacg gaaccatgtt cgatggttca
     4861 tcaattgaag gctttgtaag gattgaagaa tctgatatga ttctcaaacc ggatatagac
     4921 accttctgta ttcttccgtg gacaatggaa aaggaaaagg ttgccaggtt catctgtgat
     4981 gtttatactc ctgatggaaa acctttccca ggtgacccca gaagaatttt gagaaatgta
     5041 actgaagaac ttgctaagca cgggtacaca ggctttgctg gaccggagcc ggaatttttc
     5101 ctccttccca gagatgaagc cacaagaaaa cctaagcttg cctttatgga tgaaggcgac
     5161 tactttgacc ttctgcccat agatcttggc gaagaaacaa gaaaacagat agtggtttcc
     5221 ctcgaagaaa tgggattgaa cgtcgaagca tcccaccacg aagcagcccc ctcgcaacat
     5281 gaaatagact tcacctacgc agatatcctc aaaacagcag acaatataca gactttcaaa
     5341 ctggtagtaa agaccattgc cctcttaaga ggccttcatg ccaccttcat gcctaaaccc
     5401 ttctttggaa tcaacggttc cggaatgcac gtacatatga gccttttcag caacgcaagc
     5461 aacatcttct tctccgaaaa ctctccatac cagataagca atgaattaag atggtttgtc
     5521 ggaggaatat ttaatcacat tgatgccata acagccattg ccaatccttc tgtcaactca
     5581 tacaaaagac ttgttcctgg atatgaagcc cctgtaaata tagcctggtc tgcatcaaac
     5641 agaacagctc tcgtccgtgt accggcaagc agaggaaaag gtacaagagt tgagttcaga
     5701 agtcctgacg ctacttcaaa tccatacttg cttcttgctg ttgttttcgc atgcggtctt
     5761 gatggaatta taaacaaatg tgaaccacat gaatccgtaa atgagaatat ttatcttatg
     5821 acagaagaaa gaaaaaaaga gcttaacatc cgtcagcttc cacagaatct ccaggaatcg
     5881 attgaagctt tgaaaaaaga ttctttcatt aaaggtgtaa tgggagaaca tgttttttca
     5941 aagttcatac agctcaaaga gcaggaacat cactcattct cctgcagcgt taccgattgg
     6001 gaaattaaaa agtacctgca tcttatataa aaaataaaag ggcggatttc cgccctttta
     6061 tacattttct acaatacctg aaagttctat tttttcttct tcggttagga gcttgtctat
     6121 gtccagtatg atgatcatct cgttattcag ccttgcaact ccgcttatga atctcgaatt
     6181 tttcaacccg ccgatatcag gagtttcttc aagcacattt gcaggaatag acattacttc
     6241 cttgacttca tcaaccattg ctccgatttt cttattttcc atctttatga ctattacttt
     6301 tgacttggtt ttgtcagcaa aatctttcat tttgaatttt tttcttagat taataagcgg
     6361 aagaacttca cgtttcctga aatttattat tccttctaca tagtcagccg cctgaggaag
     6421 aactgtgact tcttcataat cggctactgc atctatcttc attatatcca gtccgtactt
     6481 ttctttgcca agattgaaag taactatctt tacttccgtc atattaatac ctcccggaaa
     6541 attagatatt tttcataagc tcttttctaa aataaattat atcacatatt actgcgttac
     6601 tgtaaagaaa ttaatttact tttctgtaag tttcccaggc atcttgaaat tagtaaagta
     6661 tctgaagcct gtgaaaaata ttaatatccc aaaaagcact cccaaaggaa tccctcttat
     6721 ctgtgagaca agaaatccta tacttgcacc atttataatc catgagaaaa gatagaatat
     6781 cttttttccg cttaaaaagc ctatcgagct tataaaggca gcagtattat aaagtacaaa
     6841 aaaaaggcca gaatttatgt cccttatgga acctataagc gtgagcacgg cagaaagaaa
     6901 aagtacaaga gtcgtaagtt tatcaaactt tttgtgtttt agggttatca gtagtatggc
     6961 tgccagagga acgagcgaaa tccagaagta aagtttttca actgtgaaaa accatccgcc
     7021 tgcagcattt aatgcaataa aaatcagcga ataaagcatt ttttcctcct attttcccaa
     7081 aagaacgtca tcaatcgttc tttctttgtc ttctctgttt ttccttgctt taattttaat
     7141 accttttata ttaagtttgt tcatgatata tacgctcaga aagtactcaa aaaagagcca
     7201 tgagacatga tctattggca acaggtaacg tttgggtttc ttaagttctt cccataacag
     7261 ctttcttcca agtataggaa tggttctgtc aaatattgct tcaaaaaagg ttacttttga
     7321 cctgtctacc agatgcgcat aggatatagg atcaaccttg aaaataggat gtatctcttc
     7381 tgattcaata agctgagtca gacacatatt ccttaccaaa ggcatctgtt tttcatagat
     7441 tctataaatt ctttctttct cctcaataaa tgaaagattt tttttctgtt gaatgtatct
     7501 tgaagatctc gtggcaggcg attcataaag tatcttcggg agatgcccgc ctgcggtcat
     7561 taagataagt tcacctatat catccctcat gcttgaaact atcgttccca ccattccacc
     7621 cagacagtag cctaacacac agttattctc agcccacatc tctttttcct tgagaaaatc
     7681 tatagtgctc ataacatcaa ccactgcgtg ctgccaaaaa ttatacatga catttatatc
     7741 aggataaaca tagcctctgc ccgaggttga attatcatca actcttgtat aatttccggg
     7801 aagaattaat attgaagcat ctatgccgct tgacgcaaga tgattcccca tccaaagcag
     7861 aaattttaca ttagagcttc ccaatccatg gagtatgagt actgatgcca tagacttctc
     7921 acgtgcctga aaattataga gctctactgt ttccgttccg cgtgcagggg ttttgtaaag
     7981 tgtgggaaac cttatatagt tgcacttgta tcctgagcct ttgtatatat atcctgaaat
     8041 aaaatcaatc ggtttttttt cataagaaaa atcaatcttc atttttaata tgctccttca
     8101 taaagttaac cgtgtctctt ataatcttaa gagtatctat atccttaagc tcaaattcga
     8161 aggtatgtcc gccgtgtctg tgaataatca gttcgctgtc aactttataa ttcaaaagac
     8221 tttcatacat atcatatgat gattttactg gaacggtttt atcttctttt ccatgtacca
     8281 aaagaacggg aaccatacgt tcacttatcc atttaatcgg agagtacatg gcgtactcgt
     8341 tgggcagctt tttcggagtg ccttttagag tagttctggt agcaattttt gcaaaaacag
     8401 aatcggttac gctgaatacc tcctgaagat cagaaggagc ataataggcg acaatggctc
     8461 ttactccctt catggcattg aaatccctga caaaagtatt ataggcagca tataacaaag
     8521 acatatgacc gcccgccgaa agtcccataa gaaaaatgtt ttcatcatca aatccgagtc
     8581 gttgtgaatg ctctcttata tatttcaaac catcgtcata atccgaaagt atatcaaaca
     8641 tggtactttt gaacccatat ctataatcta tcgaaactgc tgcaaagcct ctgcttgcaa
     8701 gataccgaca ccatgaaaca ttatttggtt gattcctgaa tccggcaatc catcctccgc
     8761 cgtgtgcgaa gaataccaca ggatattttt tccttgtttc aagcggatac catatatcta
     8821 atttgagttt atctttcttt tcctgtttat aaacataagt atatggtccg tcatactcgt
     8881 cagtctgcaa cggaagacgg ctggtcagca cttttattat aaaaagaacc accagtgtaa
     8941 taaaactata gattaatatt ataccattaa atattatact taatatgaat acacctataa
     9001 ggattattct ggctattaca aacttaaact taaccaaaaa gtactttcgt gcattactgc
     9061 ttattatttt taatttttcc attatccacc tttccttggt tttacattta ttatacccca
     9121 gcaaagcaaa aaaatactaa gttttccaga taattatttt aaatacctag ttagattatg
     9181 ataaaatttt acgggaggtg attttatgga taggcttctt atttattacg gatttccttc
     9241 ttccgtaaat catgccgata gtatccttcg ggcaacggag tatttttctt catacgatac
     9301 agtagttttt gcaggctcga gaaaaaattt caaaagcata gagtttgcag aacatataga
     9361 tcataaaaat agtgaagcca taataaaaaa tcttaaaaat aataccttcg gttatgttgc
     9421 ttgcggaaac cgcgatgggg tagattcatg ctggacaaac tccgaaatca aagatctttg
     9481 cataagatgg aaaaatatgg gtataaaagg gatttttcta gatgagctag gatacgactt
     9541 taataacttt aaaagcagga aaaccgatat tttaaatatt gtacgtaatc tttctcttag
     9601 tgtttttaca aactgctgga gtccggaaga actttttgat gactctcctg atctttttac
     9661 aaaaaatgac tattatctcc ttgaatcact ttttttcttt gctaatggag aaataacaga
     9721 gtttcagtct ttcaaagatt cttttcaaag gctctcaact gctcagcagt accgcaatgc
     9781 tttagggttc aagctggcat gtgccaactc cttcggaaaa gactataacc taattggagc
     9841 tggttttatg gatttcacta tttccgcagc cgatttcttt tcccttgatg ctctttccat
     9901 aacaaagggt aactaccatt caaatgatgt agatctgatt ccttttgaca taaaagagtc
     9961 cggatactca aaaaaattta aaataaagtt ttttttgaaa actgtgaaac gcaacgatca
    10021 gcacgacaag tatataaagt tcagaaaaga taagttttct cagcttcctg tcgaataccg
    10081 tctataaaaa aactgttttg ggtttcccca aaacagtcaa tttttgtttc ctagaatcac
    10141 ggatagatgc ctcttaattt tgtggtattg taaactctgc tgactgcaac tatgtaagcg
    10201 gctgttctca tatcagtatc gtacttctcc atctgcttta ttgtatctgc aaaagcggaa
    10261 gtcattttct gcttgagtgc ctttcttact tcgtcaattc cccagaagtg ccactgcata
    10321 ccctgtaccc attcaaagta tgaaactgta actccgcctg agtttgcaag gaagtcagga
    10381 actataaata tacctttgct aagaagtatt tcgtctccgt cgggagtgat aggtccgtta
    10441 gcgccttcaa caataagctt tgccttaacg cttgctgcgt tcctttcggt aataacttcc
    10501 tgaagagcac atggacaaag aatatcaacg tcaagagtaa gcaaatcttc gttggtaatc
    10561 tttttgtagc caggatttac agcatcaagg agaactcttc ttgccaatat cttttccatc
    10621 atttctcgca tttcttttcc agtaaatcca tcttccttgt aaagacctgt ggaagcatcg
    10681 gatataccga caacttttaa gccaagctct tcggctatca gaatggctgc atatgaccct
    10741 acgtttccga atccctgaac agcaattgta agctcattat tcttcttagt gatcttacct
    10801 tttgatctta agtactctac tgcttcaccg acactgatca taacgcctct gcctgttgcc
    10861 tcggttcttc cctgtgatcc gcctatttca aggggtttac ctgttacaac tgcaagatca
    10921 ggctgcccga tattcattga gtatgtatcc atataccatg ccattactct tccgtctgtt
    10981 cccatatcag gagcaggaat atctttttcg tcgccaacaa ttacctgtat ttcagaaaaa
    11041 tatcttcttg aaagtctttc caattcgcct tctgataatt tcttaggatc aacacatact
    11101 ccgcctttgc ctccgccgta tggaatatct gcaactgcac atttccatgt catccagaat
    11161 gcaagtgctt ttacttcgtc aaggtttacg tcaagagaat acctaactcc accctttgcc
    11221 ggacctctgg caatgttgtg ttgtaccctg tgacctgtga atacctgaat cttaccattg
    11281 tccatcttta caggaaagtt tactgtaagc tctcttttag gcttagttaa aacttctttg
    11341 taatcatctg gaagttccat gatcttagcg gctttgttaa actgtctaac tgcgctttca
    11401 tacaaactgc tcatgcttta agcctccctc tcaaaattct gtttaaccat aaaatttatc
    11461 tcggtctaca ataattttag aactttattc gttaaaaata aagtcctaaa atctaatata
    11521 tgtatgattt aacggtaaat aagctaaaat aatcaacttt aaattgattt taagcagaac
    11581 tatgaaaatt ttttcacgaa atccctttca ttgctgtttt aatccatcat caatactttt
    11641 attggccgat actgcaacag gaagttcata cttttctaat attactggaa gatatttatc
    11701 ctgaaattta tgtctatcca aatagcccat aaatacaaaa gaaagtgcgg ttgctgccaa
    11761 ccccagtaaa aacgatacat aatctccaaa attaagataa taaaataaaa cggtgaagac
    11821 aataaagaca atcaaaggta ttccataaac cagaaaagac agtttggtta ctgaaacatt
    11881 aggcagttca acaaccacat aatctccttc cttcaggtcc agtttatcaa tattttttgc
    11941 tttcactttt gtatccggac tccccataat actgcatgcg ccgctcattg cacagctttc
    12001 gcaagttacc tttctttgtg tcagcatata gacatatccg tcttttactt cttttacgtt
    12061 catcagttct ctcatcaaac cactcctaat atgttttata tacgataaaa ttactcttgt
    12121 ttaaattcaa tgataaacca taaacatcat caaatcagga ataaaatgtt acagctttac
    12181 gttgcggttt catcctatac tctaatatta tatactgata cttaaattat tttttcttgt
    12241 tcgatgagct ttccaagtct tttacgggca taatacaggc ggctttttac gctccctaat
    12301 ggtatttcga gtatcgtgga aatttcctta tagctaagtc tgtccaaatc acgcatttgt
    12361 atgataagtc tgtcaccagg cgataatttt tccattgttt ttataataga actataattt
    12421 accccttgta aagcatcatc ctgagattcg ctgctgacag aacttttcag atacttttca
    12481 ttgacatcat tctgaagatc aatggtttga atcttatctt cagaatattt ttttacataa
    12541 tctttaccga cgttaacagc tatccgatat atccatgttg aaagctttga gctgcctctg
    12601 aacttgtcta tacctctgaa aactctcaga aaaacctcct gtattgcatc ttcaatttca
    12661 cttggtctta caaaatttcg taatatgcca aaaaccatgg cagcatagtc tgaataaagc
    12721 ttttcaaatg cttttgaatc ttttttcttt aattctttga taaatttttc ctctttcaga
    12781 tctactcccc ctctcatagt acagtgctat aaggtgttac atatgctaat atcaaaaatt
    12841 atagttgtct caaagccttg ttaactattt tctggtaaaa tattacgatg agtttttttc
    12901 aggaggcaat atggcattta aaattacata tgacagcacg aggatttttg tttctgatct
    12961 ccccctaata gatgtttgcg ttgctaagga tcttctgaac agtattcagg agataccgga
    13021 aaaaagtgtt aaagagtact tctttgattt ttcaaatctt acgtatcttg acagcgccgg
    13081 tcttggagcc ctcctggcat tacatcgtaa agcatctaaa accaacggaa agattataat
    13141 taaaggcgca aaagataata taaaagagct tttcatcatc accaagctag acagcatatt
    13201 tgagcttcaa aattaatgtc ttaaaaaaat atttccggat aacttattat acatcagcag
    13261 agtctttata taaaaatctt tctcgtactt tcagacagca aagctttgta acagtttaag
    13321 ttttaagttg acaaactgtt caatatacgg tatacttata ttgacgacga gttaaaatca
    13381 attttttcct ctttggattt gaacggtgtt actgtaactg attagtatta tgtatatgtt
    13441 tactggtact aattttttaa gtacagtaag tggtttatat ttccgttgag gtatactgtt
    13501 cctttaacgt cgtttttatt ttttgttctg aagtaacttg cattctgaaa aaagaaagtg
    13561 tatcatgctt ctgttttttg tagcattaaa ggtttcttaa gtaaagaaaa taaaatgttg
    13621 tcatccaaag agggagtggc gggggtaaag ccccgccatt tgttattgtt gacagaaaaa
    13681 tattattatg ctatactata ttttaaatgt cagacaagag gtgtggatat gttaaagtat
    13741 aagctttcta taacaactac caccaacagc gccggtaaaa gacgttgtaa gttttgacat
    13801 atcaaaacaa acaatggtca gtgccggcaa aaagccggca ctgatttttt ttaggaggag
    13861 atattatgaa gagaccgctc tttcagatga cctttgattt ttacaaagca tgtcttggaa
    13921 aaaaaatcaa aggagatgaa ttttatgata attacgcagg aaaagagaaa aacgctttgg
    13981 catttatctt tgcaccttct agcaatcgct gtaaaaaaac tttatcctca agctaagctt
    14041 ggcaacggtc aggtaacaga tgaaggcttt ttttatgatt ttgacaattt gacaatcaaa
    14101 acttctgatc tgacacttat acaggacgag atgaaaaagc ttatagatga aggaatcaat
    14161 tgtgaaaata tacttcttcc ggtacaacag gctttagatt ttttctgcaa cgaaccttac
    14221 aaacttcaga ttcttgaaag aatggaaaaa actgaaaaga tctccctatg cagcatcgga
    14281 gatttttatg atatatgttc ccaaaatcat agtgataagt gcaacaatct tatagccgta
    14341 aagcttttaa gcatcggagg agcttactgg atgggaaatc caaaaaacca gatgctcaca
    14401 agaatttcag gcatagcgtt tgaaaatcaa gaagcattag aagagttcat agcctattca
    14461 gaactgctta aaaagcatga tcacagggta ataggaaaac agcttgagct tttttcattt
    14521 catccacagg ctcccggaag tgttttctgg catgaaaatg gccttatcct gcgaaatctt
    14581 attgtaagct actggagaaa agtacacagg aagaacggtt tcagggaaat aagcactcct
    14641 atacttttag attcaagctt atggaaacaa tcgggtcact gggagcttta caaagagagt
    14701 atgtatacaa ctcaaattga agaaaaagag tatgccataa agcctatgag ctgccctgga
    14761 agtattctgt actaccttga aaaaaacaga tcttacagag atcttccgct taaggttgcg
    14821 gagctgggtt tagttcacag aaatgaactt tcaggtactt tgcacggttt aatgcgggtc
    14881 agacagttta ctcaggacga tgcacatatt tttgtctgtc cgaaccagct ttccgagcag
    14941 atacagcagg taattaaaat gtccgttgat atgataggtg agtttggttt taagaatttt
    15001 tcgtttgcgg tatcggtaag aagccctgaa aaaatggcca aatatcttgg agacgatgca
    15061 gattgggata aagcagaagc tgctcttaaa tcttcacttg aagcattgaa ttatacttat
    15121 tgtatttctg agggagaagc aaaattttat ggtccctcaa tcgacattat gatcaacgac
    15181 tgtctgggaa ggaaatggca gtgcagtaca ttgcagctcg attttaatct tccgcagaga
    15241 tttgaacttg aatatacaga tgcgcatgga gaaaaacaaa cacctttcat gctccataga
    15301 accctgctgg gatcactgga acgttttatc ggaatattgc ttgaacatac caagggtcaa
    15361 ctgcctttat ggctttcgcc ggtacaggtt gctgttatgc ctataagctc aaagtattcg
    15421 gagtatggct gtactatctt cagaatgctg gaagaaaaag ctgtcagagc agtcctgatt
    15481 gatagcgaag aaagtctttc aaaacgtata cgtcagactg tacttttaaa aataccgtat
    15541 atagttgtac ttggggaaag agaagtttca aactctgtta tttctgtaag aaaggcagat
    15601 gaagatgaga gctgcgtata taacttacag gagttcatcg attatattaa aaaagtagaa
    15661 cgttatagaa tataaaaaaa gggcgtgcat caatttgcac gcctttattt tatatttgct
    15721 gctcggttcg gtttattatc tcattttgca gttccgggga aagattattg aaataatcac
    15781 tgtatcctgc aacccttact ataagattgc tgtattgctg aggattcttc tgtgctttta
    15841 gaagggtatc acggctgaca acgttaaact gtatatgatg tccgtcacgc ttaaaatata
    15901 ctcttataag atcctttatc tttactatgt tttcctggtc ttcaaagaca cttggaagaa
    15961 atttctgatt caaaagagtg ccgccagttc ttatatgatc catcttagaa gctgatttaa
    16021 taaccgccgt aggaccgttt tggtcagcat ctcgtgccgg agaaattcct tctgaaagag
    16081 gctcaaaagc ttttcttccg tcggcagaag ctccgcagac acttccaaaa tatatgtgac
    16141 aagtggttgg gagcatgtca atcctatatt tccctccttt tacattgttt ctatcggtaa
    16201 cacaagagta gaaagcttca aaaacttctt tcattatact gtctgcataa tcatcatcgt
    16261 ttccatactt atgagtatag cttttaaaaa gcttgtacgc atcgtcatat ccggaaaaat
    16321 ttttagcaag tatctcttta acctgcataa ctgaaaaatt tttttcttca aaaacgtgtt
    16381 gcttcagtga actcaggcta tccgtgatac ttcctatgcc tacaccctga atatagcttg
    16441 tgttatatct tgcgcctccg gagttatagt cactcccttt ttttatgcag tcatctatta
    16501 ccacagataa aaagggaacg ggtatatact cggaatacat cttttcaatt atcatatttc
    16561 cttttagttt tatatctata aaatgattca gctgcttttt gtagatctca aaaaactctg
    16621 tataacttcc ggcttcttca attttgatat tcagccctat ctgtttgtgg gtattggtat
    16681 cgtatccgtt atttaaggtt atttcaagga ctttaggaag attaaagtat cctgtaagga
    16741 tatatgcttc tttgccaaaa gcccctgttt cgacacatcc gcttgttcca ccgcatcttg
    16801 catcttctat gctttttccc gccagaatca tttcctggac aacagcatcg gcattaaaga
    16861 ccgacggctg tccccaacct ttttttatta catctagagc cttaagtaaa aaagcatcag
    16921 gtgatttttt acttatctga acatttgaac tgggctgcaa aagtctcatc tcatcaacaa
    16981 cctcaagaag cagatacgat aactcattaa caccgtcgga accatccgat ttgagtccgc
    17041 cgatatttat atttgcaaaa tcagtataag tactgctttc tttgagtgta acgcctactt
    17101 ttggaggagc aggctgattg ttgaacttca cccagaagca ttccaaaagc tcttttgctt
    17161 tttccctgtc aatagtacct tccatcaact gtttgctgta aaaagggaaa aggtgctgat
    17221 caagcctgcc aggattgaat gaatcccagt tgttcatctc tgaaattact cctatatgga
    17281 caaaccagta catctgaaga gcttcatgaa cagtatctgg cttatactca ggcactttcc
    17341 ggcagatgcg gcttatttcc tgaagttctt cttttctttt ttcattcttt tcttcctcac
    17401 tctttttttg tgcaagagcc gaataacgtc tggcataagt aattatggca tcgcagcaga
    17461 tatccatagc tgtaagttca tcatacttgt tcagagcatt aaaatctttt tcccaatcaa
    17521 gaagttcgat agattttttt atctcgtttt taaaatctaa aaatcctttc ctgtatattt
    17581 tatcatcagc cactgtatga ccaggtccac gctgctccat aaactcagta aacattcctg
    17641 cactgtaaca atccttccat tcctgtgaca tcatggcaag tatacgaccc cttaaagctc
    17701 tttcctgcca aaaaggaatt atcttctctc tttggatttt tttcgactgc tcatcggttt
    17761 taaaaaagat tttctctctc ttgtcaatta tttcaaaatc ttcagaacta tgacagcaca
    17821 gctcaggaaa ggtaggagta tgagcgggtg aaggccccct ttctcccact ataagctctc
    17881 cgtcatatat atctatatgt ttgttctcca taatatgttt aaatgcaagt gcccttagca
    17941 ccggaaccgg taccctgccc tcatattttt cataagcctg tgtcattaaa accgctcttt
    18001 ccatagagat tctcgcctgg acttcaagac tttctttcct aagcttttca actctttcag
    18061 tcacttcatc cacctacctc atagttccgc tttctgaaaa actcctgcac ctgtgcaaaa
    18121 gttcccggcg gaagtttgtt ctggttatct gcttgataat ttcttgctaa agcatcatat
    18181 ttgtttttag cataatcatg ataagataga agctggattt tctttatttc acagtatttt
    18241 agaatattat ccatctgttc cagctctttg aactgtgtgt tcactccatt gataagcggt
    18301 atccttacaa ggatctcact ttctaatgaa ctaagctttt taagattatc tacgataggc
    18361 tcgacagaac agcctgtata ttctatatgc ttttgattat cggcaacttt aagatcgtat
    18421 aaaaataaat ctgtatgacg agcagcttcc ttcatatact cccactggca gtagccgctt
    18481 gtttctacag ccgtatgaat attttcacgt ttaagtcttc ttagtaactc cagcgattga
    18541 gtatttgcca gaggctcacc accggaaatt gtcacacctc caccgctctg ctcataaaaa
    18601 tccatatcct tcagcacaac gtcggttatc tcatcaacat ccatttctgt tccaataact
    18661 tctctggcct ggttaggaca tatcataaca catcttccgc actttatgca ttttgaaaga
    18721 tcggttgtta ttcttccctg gtagaagtat atagcttctt ttggacatgc ttctatacat
    18781 tttccacaca ttttgcacct agaagcataa aacataagat ccttttcaaa gctctggcct
    18841 tcaggattat gacaccaaaa gcatctgagc ggacagccct tgaaaaacac cacagttctt
    18901 atgccggggc catcatgcag tgaaaaacgc tgtatcttaa aaaccatagc ttcgaccttc
    18961 cttcaaaaaa ttcggactgt ttacagatag tatttttaca gttttattgg ttctgttttg
    19021 ccagttgtgt ggcttgcggg agatataatg tgcactgtca cctggataaa gatcgtatat
    19081 ttctcctgca agatatagag taagaatacc ttcaagcaca aaaacaaact cttctccttc
    19141 gtgactgtat ttctgaattt tttcggtgtt cttctgcgga agaatctcga ctacgcgagg
    19201 aagaatattt tttgtatcct ggttggcaga caaaagataa tgaatatacc cattttcatg
    19261 tatcttcaga acctcccttt cataactgcg tacgatctct cctgccatta tgtcctcgtc
    19321 agaaaagaat tcttttatat ttgtatcaag agcctttgca atattcatga gcgagtctat
    19381 agcaactgtg gacatgccgc gttcaagctg ggaaagaaaa ccggctgaca gtcctgtttt
    19441 ttcactaaga tctttaagag tcattctgct ttttaaacga tactccttaa tttttttccc
    19501 aagagtcagt atatttttca cctctttttt aatatactaa atttttattc atcattttca
    19561 ttatataaaa ttttttgttc aaaagagcac tacctttaaa cacacattag gacattcaac
    19621 cttattaaaa gacaactaac agacattttc tatattttat atactttttt tcattttttc
    19681 attataataa attttgtttt ttcctttatt ttttttatac tctttaaaaa cactattgtt
    19741 tatttgtttt taatttatgt attgcaaaaa gataatttat atgttatact ctgaatataa
    19801 caaaaatgat ttattttact tattgacttt ttttcacgta tcactttttc atatttcaga
    19861 gtaatactaa tttaaaccag ctattttctg aaagcttcct cccattttca gaatttatgt
    19921 gatgtacttt tttccataca cgtaaaaccc ctttcgtatc tgtggtccca ccaggccaca
    19981 gatttttttt attttctagg catttcttaa aattttctta attttcttaa tatttcttaa
    20041 gatttctcta agtacgtagt tctatactaa agtcattctc agtatgaaag gagctaaata
    20101 tgatacccag atcggaattg aaacacttca ttaatttttc agattacttg gctatccgaa
    20161 acagacttaa gtttatcgct tcttacgata ccaatgccga taaaaacgga gagtacacag
    20221 tcagaagtct ttactttgag aactacaaca acaaggttct tgcagaaaaa gctcttggaa
    20281 cagatcatag agagaagtac cggatacgtc tttacaacaa taaccacaaa cttataaaac
    20341 tggaaaagaa gattaagaaa gatagattta caaccaaatt ttccaaggag ataaccaaat
    20401 ctcaatgcac cgatttaatt aacggtagtt atgactttct ttcacacagc aatattcctc
    20461 tattcaacga ttttttttca aagataaaga acgatatgct tcgacccaga acaataatag
    20521 agtataaaag gcaggctttt ttttatccgt atggaaatgt acgaattact tttgatatgg
    20581 acataaaaac tgcattggga tcagttgata ttttaaatcc agaacttgtt cttgtttctt
    20641 ctcttgagcg aaaccagata attcttgaag taaagtatga caggttctta ccccagatta
    20701 tctccgactg catccagctt aatgaaagaa ggcagatttc agcttcaaaa tatgctttag
    20761 gaagacagta tctttaattc aatattttta tataggagga gtttatatga acagtacggt
    20821 aaattttagt gatattttta agtctaactt ccttgagaag gtatcttctt tttcttttgt
    20881 tgatatgctg gttgctcttg cattagcctt tgcagtgggt cttttcatta tggcagttta
    20941 taaaaagacc ttcaggggtg tactttactc gcagagcttc tgtgtttctt taatggctct
    21001 tacccttata accactctta tcatcctggc cgttacatca aatattattt tatcccttgg
    21061 aatggtaggc gcactttcta tcgtccggtt cagaagtgcc ataaaggaac cgattgatat
    21121 tgttttcctt ttctggtcta tcagcgcagg cataattctg ggcgcaggtc tcataattct
    21181 tggcgtattc ggctcaatac taataggttt tatactgctt gtatttgtca ataagaaaag
    21241 catagataat ccttatattc tcatacttaa ctgtgaaaat gacagcactg aaaaacaagc
    21301 ccttgaattc gtatcaaata atgtaaaaaa gcatgttgta aagtcaaaaa ctgtttccag
    21361 agaaaacggt atagaaacaa ccgttgaaat aaggcttaaa agttcctcaa cagagttcgt
    21421 aaactctctt ggaaatatga gtggcattaa aaatgttgtt atggtcagct acaatggaga
    21481 gtacatggcc tgatatcggc agtaaataaa aggcggtgtg acatgatgaa agaaaaggcg
    21541 atcacagttg taactgtatt atctgtttgt tgtgcagtac ttcttattat atggctggtt
    21601 ttcttttgcg atctttcagg cttaaaaaaa tctcaaacac ctgaatatat ggtaaaaata
    21661 tttgataaaa accaaatcat agacataaat attttcatag ataaggaaaa atggactgag
    21721 atgcttgaaa atgcaaccgc acaggagtac tatacatgca atgtttctgt aaactccaaa
    21781 aaatactaca acgtcggaat aagaaccaaa ggtaatgcct cactaagcat gatcagatca
    21841 tcttcgcaac ctcaaagata cagtttcaag ctggattttg gggagtatgt gaaagatcag
    21901 actctatttg gattgcagaa gatagctctg aataatatga taggtgatgc ttcttatatg
    21961 aaggagtata tgtcatatga gatgttcagc gctcttggtg tgcctgtacc gggatatgct
    22021 tatgccagca taagtgtaaa cggtgaacct tggggatttt acattgccaa tgaagatata
    22081 gacgatactt ttgttgaaag atactacgat gatctttcca aagtaaatgt ttataaaccg
    22141 gaaacaatgg aaatgaacgg ggaagacgga caacagcctg gcggattcgg aagaaacggt
    22201 tctacgggta ccaaccttgt atacaccgat gacaatcttt caagctacag cggaatattc
    22261 gattatacgc tctttgaaac taccggtgat gatgataaga tgaaggttgt tgagatgatg
    22321 aaaagtctga gcactctttc agatattgaa aagcacatta acactgaaga agttcttaaa
    22381 tactttgcgg ttaatacttt tctggttaat cttgattctt acgcaggctc catgaagcac
    22441 aactactatc ttctggagaa tgacggccta ttcgaaattt tgccttggga tctgcacctt
    22501 tcatttggcg cttttcaaat gcagcagaca tcaagagtca taaattttcc tatagatgcc
    22561 cctgttaccg atagtatgca gaacagccct ctcatttcaa agcttctaga agtcgacaag
    22621 tacaaaacca tgtatcattc atatctacaa aagcttatac aggagtttat tgacagcggt
    22681 aagtactatg aaaggatcaa gcaggtaagt tccatgattg actcttatgt aaaaaacgat
    22741 tctatggcat tttatactta tgaagagtat caatcagctc tggaaaatat gataatttat
    22801 ggaaaagaca gaactttgag cattaaagca cagctcgaag ggtcacagcc aagcacagag
    22861 tatggaaata tccagtcatc actcaatctt acagctttgg gaaccatggg cggcggaaaa
    22921 aacgtcggag atagaatgat gaacacaaat cagcagtcac aagatttttt tgatacagca
    22981 gatgcatccg gaacgcaaat gcgacaattt caggcacctg gagagcaaaa cggtgctcag
    23041 agtatgccaa atcaattcgg acagcctcct gcagatatgc aaagtatgga gccgcctgat
    23101 aatcagggac aagaaaatat gcagcccgga aacatgcctg aaccaccgga gggattttct
    23161 tcacgtcagc ctccagagtt cagtgaaaaa agaggctttg gtccaagacc aaatcaagac
    23221 cagcccggga actttcaatt tcaaaactcc acccaagcat catcaacaac agccatatca
    23281 tccgaaacgg ctttaaattc aactcctata cttcctaatg gtcagaatat gcgcaaaaat
    23341 gaaataaaca gaaacagtcc ttttaggcaa gcagatcata ccttagaata tgcagcactc
    23401 ggagtatctt ttgcagcaat ctttatagga cttatagctg ttttcaggtt taaaaagcgc
    23461 aagtattcaa gttaaccatt atcgtcttta cggttcatcc acacaaagct cctgtttaaa
    23521 taaaaggtac agtccgataa ggctgtacct tttatttata aggcagtctt tctttattat
    23581 cttaatatga gttaatactt gttgaaagat cataacttat aattatttca aaataataaa
    23641 agaaggtgct gctttgaaaa gcattaagtt caaaatagct ttgatctcaa taatcatttc
    23701 ttctctggct tattttttga tactcgccat aacctattca accgttaccc aaagcatagt
    23761 cagccaaaac gaacagttta cacgtaaaat actgagtttg aaatcgcagc agcttgaaaa
    23821 ctatctcgat ggaataattt tacattactc aggatatata aaaaacagct ggcttataaa
    23881 ggattctctt gtccttagtt actgggatat gatcaagtca gaacttacta ctaaaattct
    23941 tgaaaaagaa gaagattttt acgatattat tttgatatct gaatccggta aagcttttag
    24001 ctctttggaa ggtgaaaaag aggatttttc agatgaaggc ttacaaattt tcaaaacatg
    24061 tcctgaagat acgtatatca gagttgaaac aaaaggttca gatacatttt tgaatgctta
    24121 cttcagagta tatgatgaaa ccttaaaagt aataggtttt agcatattaa agataaaacc
    24181 tgactttatt aaattatcag taggagatat aaacattgtc ggggttgtaa gctcatctct
    24241 tattacagat gatggcagtt tcttcagtac aactgcagac attactcaag atagaatctt
    24301 agtatcggcg gatatagctt cggaaaatgg ctggaagata ggagcatata tagataaaaa
    24361 gattttaaac gctgaacttg acagaataat acggctactt tcaataacgg tagtcttgat
    24421 aacagttatt tcaactttta tgtacatttt aaccggagcc tttttactta atcctttgaa
    24481 agatcttgaa aaaggtcttg aaataataaa acaaggaaat ttgaaaaaag atctgcgagt
    24541 aagaaccaaa gatgagatat accgaataag ctgtaaaata aatgaggtta ccgaaaaact
    24601 ccgtgacgta atggccgcca ttcttgacac tactaacgaa ctgagttctt cttcaaatga
    24661 ttttaaaata atttcccata ccttaaaaga ctcttcggac agacttcatg cagatatgac
    24721 ccaaacccac agcaaaactg accgtttcaa acttgccctg ttagattttg aaaagtatat
    24781 gcaacatctt gaatcagata tgaacgaaaa ccactcgtac tctgaaaaaa tagaagtttt
    24841 tgcgggcgat ataaaaaaag agtcttatta tggactatca aaaatcaatc aatgtcttga
    24901 tttttcagaa aaaaccattc aaaggattga actttctgat agatatgtag gagtactcgc
    24961 aaaacactcg gaatcaatag aagagatagt gcatgcaata gaaaaaataa cctcacagac
    25021 aaatatgctt gccgttaatg cttcaataca agcagccaaa gccggtgaaa acggaaagag
    25081 cttcggggtg gttgcctcag aaatacgaaa acttgcatcc gaaagtaaaa ctgcaaccga
    25141 aaaaatatat atagttttga aacagataaa ggatgatgga gataaagcca aaaatctttc
    25201 tgcggaatgt cttgaagata tacgaacaaa taattcaaat atcatggggg tttcctcaga
    25261 gttttcttca atcattgaaa aaatagttga actgtcagtg atgcttgata aaatgaagaa
    25321 aataactcag gataattttt ctgaaacctc caatattatt gttaacctaa aaaacttatc
    25381 taaagctctt gtcgttattc agtcagagat aacagaggta gcagaaacat gctccaatca
    25441 aaaagcaatc tctaaaaaga taaaagatca t
//
LOCUS       DAOSHR010000007        22712 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_1816_length_22712_cov_6.503421, whole genome shotgun sequence.
ACCESSION   DAOSHR010000007 DAOSHR010000000
VERSION     DAOSHR010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 22712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22712
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1816_length_22712_cov_6.503421"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            54..812
                     /locus_tag="PLU28_00770"
     CDS             54..812
                     /locus_tag="PLU28_00770"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HOG33626.1"
                     /translation="MKTISLARYRVFEGSLILINASFSYHDTVKKILKPIDTDNKKLF
                     LEKEAADAFLMLMSDIGGWKEIAAVSGWRSINEQHDIFSSSLIEKGLEFTQKYVAFPG
                     YSEHQSGLAIDLGLRQSDIDYIRPSFPYSGICKAFKDKAAHYGFIERYPKGKEGITNI
                     AWEPWHFRYVGCPHAEIITKLDLTFEEYHDFLKQYEYGTKSFKYANSEKLWSISYMKA
                     CAESFTNIEGDPGSTLYISGNNSDGFILTELKNK"
     gene            1041..2429
                     /gene="lpdA"
                     /locus_tag="PLU28_00775"
     CDS             1041..2429
                     /gene="lpdA"
                     /locus_tag="PLU28_00775"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOG33627.1"
                     /translation="MAYDYDIIIIGAGPGGYVAAIRAAQLGKKVALLEKDKPGGVCLN
                     VGCIPSKALIHQAQAFSSIKDLEGMGIKTDLSGFEYEKVFEKSRKIADTLSKGVQFLM
                     KKNKVELIMQQGKIESPNSVRLSDSKIISAENIIIATGSSPRVIKGFEFDEDKVLSST
                     GVLMMKKLPKSILILGSGAIGVEFAHILSSFGVKVHIVEMLDQILPIEDSEVVQVLHK
                     SFKKRGIEIYTSTKAKSMTKDEDGVTVLIEDKEGKESVIQVEKILVAVGRSPNTGDIG
                     LDKVGIETERGFIPTYDYYQTKIPSIYAIGDVINSPLLAHVASKEGEVAVEHICGHET
                     IKKIDPMLIPGGVYCEPEISSFGYNEQRAIKEGISFKKAVFPYRGAGKSVAIDKPDGM
                     VKIIYNPQTKEILGAHIVGEQATELIHELLLAKTAGIKPSEIATMIHSHPTLSETVME
                     AARAVEGWAIHA"
     gene            complement(2458..3390)
                     /locus_tag="PLU28_00780"
     CDS             complement(2458..3390)
                     /locus_tag="PLU28_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG33628.1"
                     /translation="MDTFLYAILVIFAITFISAYLKSTAKDGTLKKFSKFSIIVQTND
                     GKTVWGKMKLFSNGMELTYPKEVTVPQATRKGYYIYKTEYDKIVCIMRILKNMSYDDL
                     RLREKRNIFYKNGFFVRIRRWVRNFFAASSDAIKETLTLVMGKVGGTMSAKGQANATR
                     LGNDLIGFVGNSYEPIYEKLAGKKVLVDYLQKDGSIKKYKGILGKYTKEYIEILNIDF
                     PMDIKYEIKTRTSNITGYPVSVIRKDKKMTLVNESEQNLKFSVDGKEYSLTSKMTLDV
                     KCPDSDSFDFIVGTLENVDVILPRTLAIVRHLSD"
     gene            3535..4833
                     /gene="rimO"
                     /locus_tag="PLU28_00785"
     CDS             3535..4833
                     /gene="rimO"
                     /locus_tag="PLU28_00785"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOG33629.1"
                     /translation="MHKFHIVTLGCPKNEADMDVLRGIFESNGYKYSQDPVKSDYIII
                     DTCGFIDSAKAESIDTFFEYLELKKDNRNLKVIAIGCLVQRYFDDFIKDLVELDGLIG
                     VVPPQEVFRVIDSGEYYLKPDTPMDVYSCTSRYVPKTAYAYIKIGDGCSRRCNFCSIP
                     NFKGEPMSREIEDICHEAEFLIKSGKKELILVSQDNTLYGVDIYKKQALTELLKAIDS
                     ISGDFIIRVMYLHPDYLTDEIIDCMQSLEKVVNYFDIPMQHGSDRMLKLMGRAKSTAA
                     LEKMIAKIRMKPSVIRTSIIVGFPGETDEEFQQLKEFLNRNEFDRLGAFAYSEEEGTP
                     AAQMGGKVDEVQKQERLDELMELQKDISYDIMKKYVGRKLRVLVEEYEDGVYIGRAFM
                     DAPDIDGSVFFKSSREITLGSLVEVIIKESYEYDLEGEVI"
     gene            4830..5348
                     /gene="pgsA"
                     /locus_tag="PLU28_00790"
     CDS             4830..5348
                     /gene="pgsA"
                     /locus_tag="PLU28_00790"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HOG33630.1"
                     /translation="MIFNLANVLTLSRIFFVIPITVLTLLGESYYIPALVIFVIAAMT
                     DMFDGMAARKSNQVSDFGKIMDQVCDKILINSIFILFIAISKVPAWFVVILVARDTFV
                     NGMRTLLAGKKIVVAADKLGKLKTVLEIALIICVYSQLFGNIVESTLMYMTLIISVVS
                     GLNYIIKNLKHI"
     gene            5416..5997
                     /gene="thpR"
                     /locus_tag="PLU28_00795"
     CDS             5416..5997
                     /gene="thpR"
                     /locus_tag="PLU28_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HOG33631.1"
                     /translation="MSDNTVRTFIALESSKELGNLLDDTIDKLVKKGFKATWTKTSAA
                     HLTLVFLGDMSMKKIAEMAYKIGSRISGFPTFIYNVSKVGYFEYNSLPRVIWLGVNGG
                     PTLSGLYKEICKAITGAGINLKNEEFIPHISVGRMKFSPKDWKKVLETITFEPLSVGA
                     SSVGIYSSTLTSDGPIYKKLYKVDFEGGVIING"
     gene            5990..7120
                     /gene="recA"
                     /locus_tag="PLU28_00800"
     CDS             5990..7120
                     /gene="recA"
                     /locus_tag="PLU28_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOG33632.1"
                     /translation="MAKDNSKNDNTVEKKEEMLEKLVKELEKTHGSGSVMIMGKGVDE
                     REKIDVIPSGCLSLDIALGIGGFPRGRIIEIYGNESSGKTTLALQALAQAQKKNGIAA
                     FIDAEHALDVEYAKKLGVDIDKLIVSQPDFGEQALEIVDSLVRSNMVDMVVVDSVAAL
                     VPKDEIEGAMGDMHVGLQARLMSQALRKLAGNVNKSKTIVIFINQIRMKIGVMYGNPE
                     TTTGGVALKFYSSVRIEVRKGNQIREGKEAIGNETNIKVVKNKMAPPFREAPVDMVYG
                     RGITRENDLFNLALEANIIQRKGAWFSYFDENNNEVSLGQGKPNSVAYMVDNPDFSDF
                     IEYSIRKKNNVKIPENLLVKFEPPTDKKSKKNEQPVKNEETV"
     gene            7104..7568
                     /locus_tag="PLU28_00805"
     CDS             7104..7568
                     /locus_tag="PLU28_00805"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HOG33633.1"
                     /translation="MKKPFDPSDRENALKSALNLIRFRIRSEKELFYKLKEKGYDTDN
                     ISDTVQKLKENKLLDDRLFAKCFSYDKATLDGKGPQGIRYELKEYGISEEIIADTLKD
                     LEKEVDFYEIALSQAKKFSALKKDTKKVYDFLYRKGYDSFTIKNILKDIGGE"
     gene            7569..9122
                     /gene="rny"
                     /locus_tag="PLU28_00810"
     CDS             7569..9122
                     /gene="rny"
                     /locus_tag="PLU28_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HOG33634.1"
                     /translation="MATILGYILIPIVAAITFFVGLYIGNVKFLTKNREEKERLEEEI
                     KKAKLDAAEIKSKAIEEATAIKKREIIETREEVHKLRDEFEKESKIQRDELKSLEERL
                     VRREENIDRKEQALDEMKEKIEKEKVESEKIKDEASKKLYEIASLNAEEAREIVMKQT
                     SEKYELELAQKYRDIKDRYEDDAKKYSRWIITTAIQRYASDVTSEITTSTVILPTDDM
                     KGRIIGREGRNIRTFEKLTGTDLIIDDTPEVVALSCFNPLRREIAKRTLTMLVADGRI
                     HPARIEELYEKSKKEVEEIIKEAGKEAVMRVGIKSPHPEIVKLLGRLKFRTSYGQDVL
                     EHSIEVAMFAGMMANELGLNAELAKRAALFHDLGKAIDHEVEGSHAIVGGIIAKRYGE
                     KLEVINAMQYHHNEVDAMTPEAVLVGASDALSAARPGARRETLENYVRRIEQLEEIAK
                     SFRYVDKAYAIQAGRELRIIVQPEKVDDSISEKLARDISAKIEETMEYPGIIKVTVIR
                     EKRSVSYAS"
     gene            9229..10350
                     /locus_tag="PLU28_00815"
     CDS             9229..10350
                     /locus_tag="PLU28_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOG33635.1"
                     /translation="MDPKELRHELHRYPEVFFKEKKTTEILINALEDLERQYPGSLTI
                     KRPLETGMVIERKTNSGRYVLIRADIDALAIKEETSSPFSSLNTGFMHACGHDVHMAG
                     LYATIKKILEENIDQNFLFVFQPAEEGGGGAEKMLSTGIFDSYDIAYAMAMHVTDEYP
                     RKTVASTRGLLFASATEVDVQFIGRSAHVALAQDGRNAFTALRSYLDYTDMMPKSFKE
                     PVLYANGKMYAGSVRNIIPDYAKLEGSIRAFSIESGENFYRELSRILENVSAMTGVKG
                     VISKGSVYSEVLVDKKLYEKAEEILSEDYAYIDCGYKMTGEDFGFFSKKYPSFMFWLG
                     TRCVEERFGLHNSRFIPSDEIIDDAADIFIKLIKGFGRK"
     gene            10355..11026
                     /locus_tag="PLU28_00820"
     CDS             10355..11026
                     /locus_tag="PLU28_00820"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HOG33636.1"
                     /translation="MTNIFEGFTPQTVSFLSDIEVNNNKVWYESNKGRYKKDVLLPFQ
                     SLVAQMSWEMLNIDPEFETTPAVDKTISRIYRDIRFSKDKTPYRSNVWITFKTKTTDW
                     KNKPAFFFELFPDWYRFGMGYYTSEKNIMDRFREKIESRPAEFLMCTEFLKEKVFSVE
                     GESYKRSIYKGKNEYIAQWYDYKFFYITCNRKKDEKLFSPELAQYLTESMKKLEPMYR
                     YLKKI"
     gene            complement(11063..11956)
                     /locus_tag="PLU28_00825"
     CDS             complement(11063..11956)
                     /locus_tag="PLU28_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HOG33637.1"
                     /translation="MKSLNEVIEMAKMKGRKRVAVVGAEDVESLKAVYMAHDAGFAEP
                     VLVGDPQKIKESIAQAGLQDRDFSIVSAVSSVDAAEKGVRLVSSKSADVLMKGYIKTS
                     ELLKAVLNKEWGLRTGSVLSHVAVAETPALSKLILITDGGMLVRPTLDEKVAMINNSV
                     AIAASLEIKNPKVAVIAAVEVVNPDMPETLDAAILTQMNKRGQIKNCLVDGPLGLDNA
                     LSLMAAKIKHIEGDVAGQADILVVPDIHSGNFLGKSAIYLAGGKIAGIIAGAKAPIVI
                     ISRADEADSKLCSLALGVLNS"
     gene            12265..13821
                     /locus_tag="PLU28_00830"
     CDS             12265..13821
                     /locus_tag="PLU28_00830"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOG33638.1"
                     /translation="MFIYMIIIIITNRKKKSPVLKDFLGVIFFMIFWASGVFFELYSP
                     ELQHKLFWRNFTQIGSFGGSPMMFFFTVRFLGIDFKGLKMIKVLSAIFSGLFILLFFT
                     DNYHHLLRLNVQSAELGSFTVLLFERTILNKISVVYCSVFSIFSEIMILARYRKSSDD
                     LKKQLMIIFLGILLISVGWWNKIIWLEKNSVYFAFVFLFIPFALLILMGYFRYSLFSY
                     SPIAREKVFEIVEEGMVIFSAEGEVIDINPTAMNILVSIGYMFNLYQGNLEKYKTKEF
                     NRNIAEYIKAHHKDWYSSIINCFKETLEIKESILGHDRYFMISTYPVFDSLRNPQGSI
                     SVIKDITLQKNSAISLKIKSETDGLTGILNKESFIEYVQKYMDTKGFGSRSLFLVLDI
                     DFFKEVNDTYGHLAGDSILKDLSALIKSAIRDNDIFGRIGGEEFGIFAKEIGDMKVYR
                     YCERIRKKISRTVFKFEEKEIKITISIGCAVSQNEKRYDNVFKKADSALYRAKNNGRD
                     KCLIFSEKNT"
     gene            14458..16545
                     /locus_tag="PLU28_00835"
     CDS             14458..16545
                     /locus_tag="PLU28_00835"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HOG33639.1"
                     /translation="MKKVLVLLALVVLLVVSSFAQVSLDEAKALILKQGILKNVDKSN
                     LTYDEYKAAVAKAFPGKENLIGSGNKAVTRSDYIVALVKVLGLTSQASGYTEVVAMAN
                     DEFSVPKDAIGAVTLAYRSDRQLLNYRYGHIIDPTASITKEEAAVSLFMALNPPKKGG
                     TLTTPVGADAPGFNTLFTSAGLTWTICNIIGDGNMGTDQDGFYIPRMIKRIPTIENGL
                     IKVNSDGSMSITYELRKGMKWHDGKPVTARDAKFQWEVMVSEAPVTSNFFEKTVDKVD
                     ITDDYTFTIHLNQASGSAYFGSSVYAYYFGWFQLPEHVFRKDFESAKASGKWDDFVLK
                     VTRNPIMTGPYKFKEYKEGQYVIMEAFDDYFMGRPNVDTIVMKIIPDSDVTYASAKNG
                     EVDFGRYSLSLNQSVQLEKEHGDLFNVFYVKSVAPDCIWLNFRDSSDVTKPNKFFSDI
                     RVRQALMYAINRDAINSVVYSSKATVVDTWITDLHLMRQALKSPGINKYNYDMKKAKD
                     LLSQAGWKAGKNGILEKNGQQFSFKMIVGAGNSDYLKMAQMIQGMLKQVGISMEIDTK
                     PAVSIWELQPQGKFDANLSGWGYGIPDEALNYWTSEMIPSEANGFGGTNYGGWSNKEN
                     DVIALEAFNELDSKKKLELYEKHFAIWSKELPYLPLVAAPTPIFAKKYVKSFDAGYDN
                     GLGWIIQNWYISK"
     gene            16597..17979
                     /locus_tag="PLU28_00840"
     CDS             16597..17979
                     /locus_tag="PLU28_00840"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HOG33640.1"
                     /translation="MLFEELIAKVPDYKSFYTVDEFNERSLALAQKYPDKVKIHMGGY
                     SRKNEPIYILEIGKGSKNALLFGCPHPNEPIGAMMLDSLSEMLAKGEESLDKLDYTWY
                     IIKVIDVDGTKLNEGWFKDSSSIKKYAQHFYRPPGHEQVEWTFPIHYKTLNFDTPLPE
                     TKILMKLMEEKKPAFMYSLHNSGFGGVYYYVSGDLGSQLFDDFQALPGKFDLPLNLGE
                     PEAPYLKQLAPAIFQLFGISEEYEYLVKHLPQGTDPAKVIKAGTSSDDYASQVGNTYS
                     LVCEMPYYYDSRVDDKTELAQTRQEFLKKSLESTKQAYDHVKKILSMIESELKDKSVA
                     FYSAVSNYVEVTQDHIKATENWIENDETLRRPATVAEEFDNVYARKFYDSLMQGMLRR
                     LINENLKHDPENHVLLQAKQEADKRFEELNHFLEENLKYKTIPIKSLVCVQMIAGLNT
                     MYRLQEKGEK"
     gene            17979..18962
                     /locus_tag="PLU28_00845"
     CDS             17979..18962
                     /locus_tag="PLU28_00845"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG33641.1"
                     /translation="MKQYFLKRFLELIPVFFLISFIIFMLINLMPGDPLLQMRMDNPR
                     AVMNDPQRMQVLREYYHLDDPLIVRYFIWLKSALTGDFGYSSMYKVPVWDLIANRLPN
                     TLILTIGAWILGLIIALPIGIYSAVKKYSFFDYSTTVMAFIGISLPSFWFALVSIIFF
                     SVTLKWLPVSGMETYGMTGTWNVFWDRLKHLILPVCVLGLVQVASWVRYIRTSLLEVL
                     DQDYVRTAYAKGVPDKKVIFKHALKNAMIPIVTIIALDIPYFFGGALIIESIFSWPGM
                     GRLMYQAVIGSDYNLAISCLMFLTVITLISNLAADLIYVLIDPRIRVGKKA"
     gene            18974..19915
                     /locus_tag="PLU28_00850"
     CDS             18974..19915
                     /locus_tag="PLU28_00850"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG33642.1"
                     /translation="MGLSKLFYKNKVKEIEEKASPINILTYRKLVWMKFKSHKLALIG
                     SIILAALIIFCFFGPLFTNKSYEDMDFSAIFSPPSISSGHLLGTDELGHDVLIRIMYG
                     GRISLIVGFLSALLTTVIGTIVGLLSGFYGGIVDRFLMRFVDIMLSIPMFPILLILTM
                     VFGSGLMNIVMVLTVFGWMSISRLVRGLTLSLKESEYVISAKAVGVKNMEIILKHIFP
                     NVLPIVIVSATLNVSYAILSESSLSYLGLGIQPPMPSWGNMLQKSMNYILGTVSGTTP
                     WWLTFFPGLFIFLAVLNVNFLGDGLRDALDPKFTSNS"
     gene            19929..20933
                     /locus_tag="PLU28_00855"
     CDS             19929..20933
                     /locus_tag="PLU28_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG33643.1"
                     /translation="MSEKILEVKNLEISFKTQFGLVKPVDDVNFHINKGESLGIVGES
                     GCGKTVTAFSIIRLLPKNAVLGPATSIMFNGNDISKYSKEELQKVRGKSISMIFQEPM
                     TSLNPLFTIGQQLSEVFMLHEALNEKQAMQKSLEILEMVRIPEAKNRMESYPHMLSGG
                     MRQRVMIAMALACKPSLLIADEPTTALDVTIQAQILELINELKTIQNTASMIITHDLG
                     VIAEMCDRVIVMYAGQIVETADVYSIFDNPKHPYTQGLLNSIPVLEIAKKDQKKLHVI
                     KGYVPHPSAFPKGCRFNPRCEFASEKCISYQPHNFKISDTHTVKCWLYENSEQGDADV
                     "
     gene            20926..21924
                     /locus_tag="PLU28_00860"
     CDS             20926..21924
                     /locus_tag="PLU28_00860"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HOG33644.1"
                     /translation="MSEKKEILNVKNLKKWFQMKGQMFSNKKSFLKAVDDVSFSLNAG
                     ETLGIVGESGCGKTTVGRTLMRIYQPTCGEFWYDEKLTGQPVDIFKLKDRQMQKIRSH
                     IQMVFQDPYASLNPRMTVQDIISEGLRVNKLTSSKKETNERIADIMEKVGLRPEYMKR
                     YPHEFSGGQRQRIGIARVMIMKPKVVICDEPVSALDVSVQAQIINLLEDLKHEFDLTY
                     LFIAHDLSVVKHISDKIAVMYLGKVVESAYTDTLFENSLHPYTQGLLHAIPIPNPHTR
                     RMGKRILITGDIPSPVDPPEGCRFAKRCPKAFDRCFRETPVLTEKEKNHNVACFLY"
     gene            22103..>22712
                     /locus_tag="PLU28_00865"
     CDS             22103..>22712
                     /locus_tag="PLU28_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOG33645.1"
                     /translation="MKLKLGQKIVLILTVTQIAAFFTVLLYLGITFTDILNKQTGNAT
                     LNAQQILLKPIIWAFVIALLWIAASVFFTYRAIIKGVSLPLKYLSSQVVDFGNGNLKV
                     KFRQKTQDSVGQMVGALRSMTENLHGNMIKIKEIGEQLNESSEKLQNASESSEKHVTK
                     LQGDISDFTAGISESTESIIDINTSIDRISKSSLTISNDSQNL"
BASE COUNT         7499 a         3899 c         4812 g         6502 t
ORIGIN      
        1 ggaataactt ttgaaaaact catttctgat ataatccgac tggggttgga agcatgaaaa
       61 caattagctt ggccaggtac cgtgtttttg agggaagtct gattttaata aatgccagtt
      121 tttcatatca tgatacagta aaaaaaattc ttaaaccaat agatacagat aataaaaagc
      181 tgtttcttga gaaggaggcg gcagatgctt ttttaatgct tatgagcgat atcggcggct
      241 ggaaagaaat agccgcagtc agtggctggc gttctatcaa cgaacagcat gatattttta
      301 gcagttctct gatagaaaag ggtctggagt tcactcaaaa gtatgtggca tttccaggat
      361 acagcgagca tcaatctggt ttagcaattg atctgggatt aagacaatct gatatagatt
      421 acatacggcc atcgtttccg tattccggta tttgtaaggc ttttaaagat aaagccgctc
      481 attatggttt tattgaacgt tatcctaaag gcaaagaggg gataaccaat atagcatggg
      541 agccatggca ttttagatat gtcggctgtc cgcatgccga aataattact aagcttgatc
      601 ttacttttga ggagtaccat gactttttaa aacagtatga atatggcaca aaatctttta
      661 aatacgcaaa ttctgaaaaa ttgtggagta tttcttatat gaaagcttgt gctgaaagtt
      721 ttacgaatat tgaaggagat ccgggttcaa cgctttacat atcaggtaac aattctgacg
      781 gcttcatact cacagaattg aaaaacaaat aaaagcagtg ataagttgtt taatcatgaa
      841 agaaaagtta taatatgatc aatgtaggat gatttgaaat atcttgcatt taaaaacaaa
      901 tgtcagagag tctgacattt gttttgttag ttaaccaagt taattgtatt tgtgtaattt
      961 tatttttaca tatattgtat atatttaact ttgagcactg caatgcagtg aaaataaaaa
     1021 caaaaaagga gaggctgaag atggcatacg attacgatat aatcattatt ggagctggtc
     1081 cgggcggata cgttgcggcc ataagggcag cccagctggg gaaaaaagtt gcattgctgg
     1141 aaaaggataa accaggtgga gtatgcctca atgtaggatg tataccgtca aaagcgctta
     1201 tacatcaggc acaggctttc agcagtataa aagatcttga aggtatgggg ataaaaacag
     1261 atctttccgg gttcgaatat gaaaaagttt ttgagaaatc aaggaaaatt gcggataccc
     1321 tttccaaagg ggtacagttt cttatgaaaa aaaacaaagt tgagcttata atgcagcaag
     1381 gtaagataga atcgccaaac tccgtgagac tttccgattc taaaatcata tccgcagaga
     1441 atataataat agcaaccggg tcatctccca gagtcataaa aggatttgag ttcgatgaag
     1501 ataaggtgct ttcatcaacc ggtgtgctaa tgatgaaaaa gcttcccaaa agtattctta
     1561 ttcttggcag cggtgcaatc ggagtggagt ttgcgcatat tctcagttcc ttcggtgtaa
     1621 aggtgcatat tgtagagatg cttgatcaga tacttccaat tgaggacagt gaggttgttc
     1681 aggttcttca taagtcattc aaaaagaggg gtattgagat ctatacttcc acaaaagcca
     1741 aatcaatgac caaagacgaa gatggagtaa cggttctgat tgaggataaa gaaggcaagg
     1801 aaagtgttat acaagttgaa aaaattcttg tggccgtagg cagaagtccc aatacagggg
     1861 acatcggtct tgataaggta ggcatagaaa ccgaaagagg ttttattcca acgtatgatt
     1921 actatcagac caagattcca agcatatacg ccataggaga tgtcataaac tctccgcttc
     1981 ttgcacatgt tgcatctaag gaaggagaag ttgccgtcga gcatatctgc ggacatgaaa
     2041 ctataaagaa aatagaccca atgcttattc cgggaggagt ttactgcgag ccggaaattt
     2101 caagctttgg atacaatgag cagagggcta ttaaagaggg cataagcttt aaaaaagctg
     2161 tttttccata cagaggtgca ggaaagtctg ttgccataga caaacccgat ggaatggtaa
     2221 aaattattta taacccgcag acaaaggaga ttcttggagc acatatagtc ggagaacagg
     2281 ctaccgaact catacatgag ctgctgctgg caaaaactgc cgggatcaag ccttctgaaa
     2341 tagcaactat gatccattcg catccgactc tttctgaaac tgttatggaa gcggcaagag
     2401 cagtcgaagg ttgggcaatc cacgcataaa aaaagcagcc tctggctgct tttttcatta
     2461 atcgctgaga tgccttacaa ttgccaaagt tcttggaaga ataacgtcaa cattttctaa
     2521 cgttcccact ataaagtcaa agctgtctga atctggacat ttgacgtcaa gagtcatctt
     2581 tgaagtgaga ctgtactctt ttccatcaac tgaaaatttg aggttctgtt cagactcgtt
     2641 cactaaggtc atttttttgt ctttgcggat aacggagacc ggataacctg ttatattgct
     2701 tgttctggtt tttatctcgt attttatatc cattggaaag tctatattca aaatttctat
     2761 gtactcttta gtgtactttc cgagaatacc cttgtatttt ttaattgaac cgtctttctg
     2821 aagataatcc acaagcactt tttttcctgc aagtttttcg taaattggtt catatgagtt
     2881 gcctacaaaa cctataagat cgttcccaag gcgggttgca ttagcctgtc cttttgccga
     2941 catagttcct cccacttttc ccattacgag tgttagagtt tcttttatag cgtcggaaga
     3001 ggccgcaaaa aagtttctta cccatcttcg tattcttacg aaaaaaccgt ttttataaaa
     3061 tatatttctt ttttctctaa gccttagatc atcataagac atatttttaa ggattctcat
     3121 gatacataca atcttatcgt attcggtttt gtatatatag tatccttttc ttgtagcctg
     3181 gggaaccgtc acttctttgg gataggttag ctccattccg tttgaaaaga gcttcatttt
     3241 tccccatact gtctttccat cattggtttg tacgatgata ctaaattttg aaaatttttt
     3301 aagagttccg tcctttgcag tgcttttaag gtatgcactt atgaaagtta tagcaaatat
     3361 tacaagaatt gcatataaaa aagtatccat ataaacaccg tcctttaaat tgatattaca
     3421 attataccac aaatgaaaca gaaaaaatat tgttatactt ataggaaatt cttgtgtttt
     3481 ttgctataat aatattgtga taatctaatt aattctatta cggaggaaat accattgcac
     3541 aagtttcata tagtcaccct tggctgccct aaaaacgagg cagatatgga tgttctaagg
     3601 ggaatatttg agtcgaacgg atataagtat tcacaagatc ctgttaagtc tgattacata
     3661 ataattgaca catgcggctt tatagactcg gctaaagcag agtctataga tacttttttt
     3721 gaatatcttg aactcaaaaa agataacaga aatcttaagg tcatagctat aggatgtctt
     3781 gtacagcgat actttgatga ttttataaaa gatcttgttg agcttgacgg acttataggt
     3841 gttgttccgc ctcaggaagt attcagggta atagacagcg gagagtatta tttaaagccg
     3901 gatactccta tggatgttta cagttgtaca tcaagatacg tgccaaaaac agcttacgca
     3961 tatataaaaa taggggatgg ctgcagcaga agatgcaact tttgttccat acctaatttc
     4021 aaaggcgagc ccatgagcag ggagattgaa gatatctgtc atgaggcaga gtttcttata
     4081 aaaagcggta aaaaagagct tatactggta tcacaggata atacgcttta cggcgtggat
     4141 atttataaga aacaggcact taccgaactt ttaaaagcta ttgacagtat cagcggggat
     4201 tttatcatac gtgtcatgta tctgcatccg gattatctga cggatgagat aatagactgc
     4261 atgcagtcgc ttgaaaaggt tgtgaattac ttcgacatac ctatgcagca cggttcggac
     4321 agaatgctta agcttatggg gcgtgccaaa agcaccgcag cccttgaaaa aatgattgca
     4381 aaaataagaa tgaagccatc ggtgataaga acctccataa tagtagggtt tcccggtgaa
     4441 accgatgagg agttccaaca gcttaaagaa tttcttaacc gaaatgagtt tgacaggctt
     4501 ggtgcttttg cttattccga agaggaaggt acccccgcag cccaaatggg cggaaaggtt
     4561 gacgaggttc aaaaacagga aaggcttgac gaacttatgg agctgcagaa ggatatatct
     4621 tatgatatca tgaaaaagta tgttggacgt aagctcagag tattagttga agagtatgag
     4681 gacggggttt atattggaag ggcgtttatg gatgctcctg atatagacgg aagtgttttt
     4741 ttcaaaagtt ccagagagat tactttggga agtttggtgg aagttattat aaaagagagt
     4801 tacgaatatg atttggaagg agaagtgata tgattttcaa tctggctaac gttcttacgt
     4861 tgtcgcggat attcttcgta attccgataa cggttctgac tctgctcggt gaaagttatt
     4921 atataccggc gcttgtaatt tttgttattg cggctatgac agatatgttc gatggtatgg
     4981 cagcaagaaa gtcaaatcag gtaagtgatt ttggcaaaat tatggatcag gtgtgcgata
     5041 aaatacttat aaacagcata tttatccttt ttatagctat ttccaaggtg cctgcttggt
     5101 ttgttgtgat acttgttgca agggatactt ttgttaacgg aatgagaacg cttctggcag
     5161 ggaaaaaaat agttgttgca gcagataaac tgggaaaact taagacagtt cttgagattg
     5221 cccttataat atgtgtatac tcccaattgt tcggaaacat agttgaaagt acactgatgt
     5281 acatgacatt gattataagc gtggtatccg gtttaaatta catcattaaa aacctgaagc
     5341 atatttaagc atttatgatt ttatttttaa agaaggtgtt aagctgaaag atatttatta
     5401 agagggggaa ttgccatgag cgacaatact gtacgcactt ttatagcttt ggaatcaagc
     5461 aaggagcttg gtaatcttct tgacgatacc atcgataaac ttgttaaaaa aggatttaaa
     5521 gccacatgga caaaaacaag tgccgcgcat cttacgctgg tatttcttgg agatatgagt
     5581 atgaaaaaaa tagccgagat ggcttacaaa ataggttcaa gaatatcagg atttcctact
     5641 tttatctata atgtcagcaa ggtcggatat tttgagtata atagtcttcc tagggttatc
     5701 tggcttggag ttaacggcgg gccgacactt tccggtcttt ataaagagat atgcaaggct
     5761 ataacaggag ccggaataaa tttgaagaac gaggagttta ttcctcatat atctgtcggg
     5821 agaatgaagt tttcacccaa ggattggaaa aaggtacttg aaactattac ttttgaacca
     5881 ctttctgttg gggcaagttc tgtgggaatt tactcgtcga ctctgacaag tgacggaccc
     5941 atatataaga aactgtataa agttgatttt gaaggaggtg tgattattaa tggctaaaga
     6001 taattcaaaa aatgacaata cagtcgaaaa aaaagaggaa atgctcgaga aattggttaa
     6061 ggagcttgaa aaaacccatg gttctggttc tgtaatgatt atgggaaaag gtgtggatga
     6121 aagagagaag atagatgtga tacccagtgg ctgtctttcg ttggatattg ccctgggaat
     6181 cggaggattt ccaagaggaa ggataattga gatatacggc aatgaatcat cgggaaagac
     6241 cacacttgct cttcaggcgc tggcacaggc tcagaaaaag aacggaattg ctgcattcat
     6301 agatgcagaa catgctcttg atgttgagta tgcaaaaaaa cttggtgtcg atattgacaa
     6361 gcttattgta tcacagccag actttggaga acaggcactg gaaatagttg attcacttgt
     6421 aaggtcaaat atggttgata tggtcgtagt tgattcggtc gcagctcttg tcccaaaaga
     6481 tgaaatagaa ggagccatgg gtgatatgca tgttggactt caggcaaggt tgatgtccca
     6541 ggctctgagg aagcttgcag gtaatgtaaa caagtcgaaa accattgtaa tttttataaa
     6601 ccagataaga atgaagatag gagttatgta cggaaaccca gaaaccacga ctggaggagt
     6661 tgcacttaaa ttttactcca gtgtgaggat agaagtaaga aagggaaatc agataagaga
     6721 aggaaaggaa gcaataggaa acgaaaccaa tataaaagtt gtgaaaaata agatggcgcc
     6781 gcctttcagg gaagcaccgg tcgatatggt ttacggccgt ggaatcacaa gggagaacga
     6841 tctgttcaac ctggcgctgg aagcaaacat aatacagaga aaaggagcat ggttttcata
     6901 ttttgatgaa aataataatg aagtcagtct cgggcaggga aaaccgaact cggttgccta
     6961 catggttgac aatccagatt tttctgattt catagaatac agcatcagaa agaaaaacaa
     7021 tgtaaaaata cctgaaaatc tgcttgtgaa gtttgaacct ccaacagata aaaaatcaaa
     7081 gaaaaatgag caaccagtga aaaatgaaga aaccgtttga tccaagtgat cgggaaaacg
     7141 ccttaaaaag tgcgcttaac cttatcagat tcaggatcag atcagaaaaa gagctttttt
     7201 ataagcttaa ggaaaaaggt tatgatacag ataacataag cgatacagtc caaaaactaa
     7261 aagaaaacaa gctgctggat gatagacttt tcgccaaatg cttttcatac gacaaagcta
     7321 ctcttgacgg aaaaggtccg cagggaataa gatatgagct taaagagtat ggtatatccg
     7381 aagagataat tgcggatact ttaaaagacc ttgagaaaga agttgatttt tacgaaatag
     7441 ccctttctca ggcaaaaaaa ttttcagcgc tcaaaaaaga cacaaaaaaa gtgtatgatt
     7501 tcctttacag aaaaggatat gactctttta caataaaaaa tattttgaaa gatataggag
     7561 gtgaataaat ggctacaatt ttaggatata ttctgatccc catagtggca gcaataacct
     7621 tttttgtagg tttatacata ggcaatgtaa agtttttgac aaaaaacaga gaggaaaagg
     7681 aaagacttga agaagagata aaaaaagcaa agcttgatgc ggcggagata aaaagcaaag
     7741 ccatagaaga agctacggcc ataaagaaaa gagagataat agaaactagg gaagaggttc
     7801 ataagctcag ggatgagttc gagaaagaga gcaaaataca gagggatgag cttaaatctc
     7861 ttgaggaaag acttgtaaga agagaagaaa acatagacag aaaagaacag gctcttgatg
     7921 aaatgaaaga aaaaatagaa aaagaaaaag ttgaatctga gaaaataaag gatgaagcat
     7981 caaagaaact ttacgagata gcttcactaa atgcagaaga ggcaagggaa atagttatga
     8041 aacaaacatc cgaaaagtac gagcttgaac ttgcccaaaa gtacagagat ataaaagaca
     8101 ggtatgagga cgatgccaaa aaatactcaa ggtggataat cacgacagcc atacagagat
     8161 acgcatcgga tgttacttct gaaataacca cttctaccgt cattctcccg acagacgaca
     8221 tgaagggaag gatcatcgga agggaaggaa gaaatatcag aacatttgaa aaacttaccg
     8281 gaacagatct tataatcgac gatacacctg aagttgttgc gttatcctgt ttcaaccccc
     8341 tcagaaggga aattgccaag agaactctta ccatgcttgt tgcagacgga aggattcatc
     8401 cggcacgaat agaggaactt tatgaaaagt ccaaaaaaga agttgaagaa ataataaagg
     8461 aagccggaaa ggaagctgta atgagagttg gaataaagag tccccatccg gaaatagtaa
     8521 agcttttggg aagacttaag ttcagaacaa gttacggtca ggatgtgctt gagcactcca
     8581 tagaagtagc aatgtttgcc ggaatgatgg ccaatgaact gggtctgaat gctgagctcg
     8641 ccaagagagc tgctcttttc catgatcttg gcaaagccat agatcatgaa gttgaaggat
     8701 cgcatgccat tgttggtgga ataattgcca agagatatgg agaaaagcta gaagtaataa
     8761 atgcaatgca gtaccatcac aatgaagtgg atgccatgac ccctgaagcg gttcttgtag
     8821 gagcatcaga tgctctttct gccgcaagac ctggcgcaag gagagaaaca cttgaaaact
     8881 atgtaagaag aatagaacag cttgaagaaa tagccaaatc cttcagatat gtggataaag
     8941 catatgctat tcaggcggga agagaactga gaataattgt tcagcctgaa aaggttgacg
     9001 attccatatc cgaaaaactt gcaagggata tatcagccaa gatagaagag acaatggagt
     9061 atccgggcat aataaaggta acggtaataa gggaaaagag aagcgtatcc tacgcaagct
     9121 aataaaaaca agacggcttt ttagccgtct tgtcttaata aaggagcagt ttttaggtgt
     9181 ggcagtaaag taaaaaaact ctatattggc tgaaacgagg aggcaataat ggacccaaaa
     9241 gaactcagac atgagcttca caggtatccg gaagtatttt ttaaagaaaa aaagacgaca
     9301 gaaatactta taaatgcttt agaagatctt gaaagacagt atccgggaag tcttacaatc
     9361 aaaagaccgc tggagacagg tatggtgatt gagcgaaaga caaacagcgg cagatacgta
     9421 cttatacggg cagatataga tgctcttgcc ataaaagaag agacatcatc tcctttttca
     9481 tctctaaata ctggatttat gcacgcatgt ggtcatgatg tacacatggc aggtctttat
     9541 gcaaccataa aaaaaatatt ggaagagaat attgaccaaa actttttgtt tgttttccag
     9601 cctgcggaag aaggcggtgg aggggcagaa aagatgctca gtaccggcat atttgacagt
     9661 tatgatatag cttatgccat ggctatgcat gtcaccgacg agtatccgcg aaaaacggtt
     9721 gctagtaccc gtggcctttt gtttgcaagt gccacggaag tagatgtcca gtttataggg
     9781 cgcagcgccc atgtggctct tgctcaggac ggaagaaatg cctttacagc ccttagaagt
     9841 tatcttgatt atacagatat gatgccaaag agttttaagg aaccggttct ttacgccaat
     9901 ggaaagatgt atgccggaag tgtgaggaac attattccag actatgcaaa gcttgaagga
     9961 agtatacgtg ccttcagtat agaaagcgga gagaacttct accgcgaact ttcgcgcata
    10021 cttgaaaacg tatctgctat gaccggagtt aaaggagtaa taagcaaagg ttctgtttac
    10081 agcgaagttc tagtggacaa gaagctttat gaaaaagccg aagaaattct ttcagaagac
    10141 tatgcctata tagactgtgg gtataagatg acaggagagg actttggatt tttctccaaa
    10201 aaatatccat cattcatgtt ctggctgggg acgcgttgcg tagaagaaag attcggactt
    10261 cataacagca ggtttattcc aagtgacgag attatagacg atgctgccga tatattcata
    10321 aaacttataa aaggatttgg caggaagtga tggtatgacg aatatatttg aaggctttac
    10381 ccctcagaca gtttcttttc tttctgatat tgaagttaat aacaataagg tatggtatga
    10441 aagcaataaa gggagatata aaaaagatgt cctcttaccg tttcagagtc ttgttgctca
    10501 gatgtcctgg gaaatgctca atattgatcc ggagtttgaa actactcctg cagttgataa
    10561 aaccatatcc agaatataca gggatatacg cttttccaaa gataaaacgc catataggtc
    10621 aaatgtatgg ataaccttca aaaccaaaac aacagactgg aaaaataaac cggccttctt
    10681 ttttgagctt tttccagact ggtacagatt tgggatggga tactatacat ctgaaaaaaa
    10741 tattatggac agattcagag aaaagataga gagcagaccg gcagaatttt taatgtgtac
    10801 agagtttttg aaagaaaaag ttttctccgt tgaaggagag agttacaaga gaagtatata
    10861 taaaggtaaa aatgagtata tagcacaatg gtacgactat aagttctttt acataacctg
    10921 caacaggaaa aaagacgaaa aacttttcag tccagagctt gcacagtatc ttacagaaag
    10981 catgaaaaaa cttgagccta tgtaccgtta tctgaaaaaa atataaacat gtccggtcta
    11041 atgaccggac atgcctgtta ttttagctgt taagtactcc tagtgccagg gaacaaagtt
    11101 ttgaatcagc ctcatcggca cgtgatatta ttactatcgg agcctttgcc cctgctatta
    11161 ttcctgctat ttttccaccc gcaagataaa tggcggactt tccgaggaaa tttcctgaat
    11221 gtatatcagg aacaacaaga atatctgcct gtcctgccac gtcaccttca atatgcttaa
    11281 tctttgccgc cattagtgac aaagcattat caagacctaa aggtccgtcc accaggcagt
    11341 tttttatctg accccttttg ttcatctgcg tgaggattgc tgcatccaga gtctcgggca
    11401 tatcaggatt gacaacctca actgccgcaa taacagcgac tttcggattt tttatttcaa
    11461 gacttgcagc gatagctacg ctgttattta tcatagccac tttttcatcg agtgttggcc
    11521 gtacaagcat tcctccgtca gtgataagaa tcagtttcga aagtgcaggt gtttcagcca
    11581 cagccacatg agaaagaaca cttccggttc taagacccca ttccttgttt aggacagctt
    11641 tcagaagttc tgatgttttt atgtatcctt tcatgagaac atccgcactt ttggaggaaa
    11701 caaggcgaac acccttttct gcggcatcaa ctgagctgac tgccgatact atgctgaaat
    11761 ccctgtcctg taatcccgct tgggcaatac tttctttaat cttctgagga tctcccacaa
    11821 gaacaggctc ggcaaatcct gcatcgtgag ccatataaac cgctttcaga gattccacat
    11881 cttcagcgcc cacaaccgca actctttttc tgcccttcat ttttgccatt tcaattactt
    11941 cattaaggct tttcatattg tgacctccta tgaaatattt ttcataccta ttctacaata
    12001 ttttatagct aaaaacaagc tagtaagata tatttctaaa acattaatgt tgtgcatcac
    12061 aaaacaaaaa acagtatgca aaagtgcaag aaaatgaaag attatggtat aatttcttta
    12121 aaaaatatga agtaaaaaga tataacagta aaactttatt gaagttacct aatctgcata
    12181 aaatattgaa aatatacttt tgatagtcat gtgaggggaa gagtcttgga tagggcattg
    12241 cttttgatgc tgtatgtact tagtatgttc atatatatga ttataataat tataactaat
    12301 aggaaaaaga agagcccagt gcttaaagat tttctgggag ttattttttt catgattttt
    12361 tgggcaagcg gagttttctt tgaactttac tctccggagc ttcagcacaa actcttctgg
    12421 cgtaatttca cacagatagg ttcttttggc ggttcgccta tgatgttttt ctttactgta
    12481 agatttcttg gtattgactt taaaggtttg aaaatgatta aagttctttc agcaatcttt
    12541 tccggacttt ttatcctgct attttttaca gataactatc atcatctttt gagactcaat
    12601 gtacagtcgg cagaactcgg cagttttact gttctcttgt ttgaaagaac cattttaaat
    12661 aaaatttcag ttgtttactg ctcggttttc agtatttttt cagagattat gatactggca
    12721 agataccgta aaagttctga tgatctgaaa aaacaactta tgattatttt tttaggtata
    12781 cttcttatct ccgtaggctg gtggaacaag ataatttggc ttgaaaaaaa cagcgtatac
    12841 tttgcttttg tatttttatt cattccgttt gcactgctta tactcatggg atactttcga
    12901 tacagcttgt tttcttactc gcctattgca cgtgagaagg tctttgaaat agtagaggag
    12961 ggaatggtta tcttttctgc ggaaggtgag gttatagata tcaatccgac ggccatgaat
    13021 attcttgtga gcataggcta tatgttcaac ctttaccaag gaaatcttga aaagtacaaa
    13081 acaaaagagt tcaacagaaa tattgcagaa tatataaaag cccaccacaa agattggtac
    13141 tccagcataa ttaactgttt caaggaaaca ctagagataa aggagagtat cctgggccat
    13201 gatagatact ttatgataag tacctatcct gtttttgact ctcttagaaa tcctcaggga
    13261 agcatatcgg tcataaaaga tattactcta cagaaaaaca gtgcaatatc cttgaagata
    13321 aaatcagaaa cagatggtct tacgggaata ctcaacaagg aatcattcat agagtacgtt
    13381 caaaagtata tggatactaa aggttttggc tcccgttctc tttttcttgt gcttgacata
    13441 gatttcttca aggaagtaaa cgatacgtac ggccaccttg ctggggacag cattcttaaa
    13501 gatctttctg ctcttataaa atctgctata agggataacg atatatttgg caggataggt
    13561 ggagaagagt tcgggatttt tgccaaagag atcggagata tgaaagttta cagatactgc
    13621 gaaagaatta gaaagaaaat atcccgtact gttttcaaat ttgaagaaaa agaaataaag
    13681 attacaataa gcatcggatg tgctgtttcc cagaatgaaa aaaggtatga caatgtcttc
    13741 aaaaaggcag attcagcact ttacagagcc aaaaacaatg gacgggacaa gtgtcttatt
    13801 ttcagcgaaa aaaatacata aatctgtgaa aaaagattca cagatttttt ttaagaaaaa
    13861 aaattcaaaa agttctgagt gaaaaaatat agggttttta ataaggaaat ttttgcacac
    13921 aaaaaaatat taataaaata aagatattta aaataaaatg tcttttttgg ataaattgat
    13981 taaattaaaa actgtataaa aaaatctagt gattaaacat tttataacct tttcataaaa
    14041 attattgaaa aaaacttcac accaattatt tgtacggcaa actaattaag taaacatttc
    14101 ccgtcatctg ttaaaattct gctcttcaag tatttttttt cgttgaatat ttttataaac
    14161 acgtatgaaa aaactttcat agaaaagtac tatgtgacaa aagtgcaaaa aaaattacat
    14221 aaatagtgat tttgattaca gcaagaggat atggtagata aaagaagaaa tatgtcttta
    14281 ataataatta tttttcttta aatggtctta tttggctata aaacgttttt tttatatcaa
    14341 aaaaaattgt gttataatcg ttccaattaa ttagtttggt ttaaatacta agttcacctc
    14401 atttgcagtc taataaaagg tttggttcat catattaagt ataacggggg tgtcaaagtg
    14461 aaaaaggtac ttgttttact tgctctggta gttcttttgg tagtaagttc ctttgcacag
    14521 gtttctttag atgaagcgaa agctctgata ctgaaacagg gaattctgaa aaacgttgat
    14581 aagagcaatc ttacctacga tgagtataag gcagccgttg caaaagcatt tcccggaaaa
    14641 gaaaatctta ttggctcggg aaataaagct gtaaccagaa gtgactatat agttgctttg
    14701 gtgaaggttc ttggtttgac ttcacaggcc agcggatata cagaagtagt tgcaatggcg
    14761 aacgatgaat tttcagttcc gaaagatgct ataggtgctg ttactttggc atacaggagc
    14821 gataggcagc tcctaaacta cagatacggg catattattg acccaaccgc ttccataacc
    14881 aaggaagaag cagctgtttc attattcatg gcactcaatc ctcctaagaa aggcggaacg
    14941 cttaccacac ccgtaggtgc agacgcacca ggcttcaata ctctcttcac ttctgcagga
    15001 cttacctgga caatctgtaa tataataggc gacggaaata tgggaaccga tcaggatggt
    15061 ttttatatac caagaatgat aaaaagaata ccgaccattg aaaacggtct tataaaagtt
    15121 aacagcgatg gttcaatgag cataacctat gagctcagga aaggaatgaa atggcacgac
    15181 gggaagcctg taaccgccag agatgcaaag ttccagtggg aggttatggt atctgaagca
    15241 cccgttacat caaacttttt tgaaaagact gttgataaag tcgatataac cgatgactat
    15301 acatttacca ttcatttgaa ccaggcatct ggttctgcat acttcggatc ttctgtttat
    15361 gcatactatt ttggctggtt ccagcttcca gaacatgttt tcagaaaaga tttcgaaagt
    15421 gccaaggcat ccggaaaatg ggatgatttt gtgcttaagg ttacaagaaa tcctataatg
    15481 acaggaccat acaagttcaa agagtacaaa gaaggtcagt acgtaataat ggaagcattc
    15541 gacgattact tcatgggaag accgaatgtt gataccatag tcatgaagat tattcctgat
    15601 tctgacgtca catatgcttc agcaaaaaat ggcgaggttg atttcggacg atactctctt
    15661 tcacttaatc agtctgttca gctggaaaaa gagcatggag atcttttcaa tgttttctat
    15721 gtcaagagtg tagctcccga ctgtatatgg ttaaatttca gggattcttc agatgttacc
    15781 aagcctaata agttcttcag cgatatacgt gtaaggcagg ctttgatgta tgcgataaac
    15841 agggatgcaa ttaacagtgt tgtatacagc agtaaagcga cagttgtcga tacatggata
    15901 accgatctgc atcttatgag acaggctctt aagtctcctg gcataaacaa gtataattac
    15961 gatatgaaaa aagctaagga tcttctgtca caggcaggat ggaaagccgg gaaaaacggt
    16021 attcttgaga aaaatggaca gcagttctca ttcaagatga tcgtaggcgc aggaaacagc
    16081 gattatctca agatggccca gatgattcag gggatgctga aacaagttgg aatatcaatg
    16141 gaaatagata ccaaacctgc ggtttccata tgggaactcc agcctcaggg aaagtttgat
    16201 gccaatctat ccggatgggg atacggtata ccagacgaag ctcttaacta ctggacttct
    16261 gaaatgattc cttctgaggc taacggattt ggtggaacca attacggcgg ctggtcaaac
    16321 aaagaaaatg atgtaatagc tttagaagca ttcaatgagc ttgacagtaa gaaaaaactt
    16381 gagctttacg agaaacattt tgctatatgg tcaaaagaac ttccttatct gcctttggtt
    16441 gccgctccta cccctatttt tgccaaaaaa tatgtcaaat cttttgatgc agggtacgat
    16501 aacggtttag gttggataat tcagaactgg tacatttcaa agtaaaatat tcgggcgggg
    16561 aaacccgccc ttataataaa aaaggggtgc actctaatgt tatttgaaga attaatagca
    16621 aaggttccag attataaaag tttttataca gttgacgaat tcaatgaaag atctcttgca
    16681 ctagcacaga agtatccaga taaggtaaaa atacacatgg gagggtactc aagaaaaaat
    16741 gaacccatat acattcttga aataggtaaa ggcagtaaaa acgcacttct ttttggatgt
    16801 ccacatccca atgaaccgat aggagcaatg atgctcgaca gcttgagcga gatgcttgca
    16861 aaaggtgaag aaagtcttga taagctagac tatacatggt atataataaa ggttattgat
    16921 gttgacggca ccaagttgaa tgagggatgg tttaaagaca gttcgtccat aaaaaagtat
    16981 gcgcagcatt tttacaggcc accaggacac gaacaggttg aatggacatt tcctatacac
    17041 tataaaactc ttaactttga tacacctctt cctgagacta agatccttat gaagcttatg
    17101 gaagaaaaaa agccggcgtt tatgtactca ctgcataatt ctggtttcgg aggtgtttat
    17161 tactatgttt ccggtgacct tggcagccag cttttcgatg atttccaggc tcttccggga
    17221 aagtttgatc ttccccttaa tctcggggaa cctgaagcac cttacctaaa acaacttgct
    17281 cctgcaatat tccagctttt tggaatctca gaagagtatg aatatcttgt taaacatctt
    17341 ccccagggaa ctgatcccgc taaagtaata aaggcaggta caagctctga tgactatgcc
    17401 tcgcaagttg gaaataccta ctctttggta tgtgaaatgc catactacta tgattccaga
    17461 gtagatgata agacagaact tgcacagaca agacaggagt ttctcaaaaa atctcttgaa
    17521 agtactaaac aagcttacga tcatgtgaag aagattctga gcatgataga atctgagctc
    17581 aaggataagt ctgtcgcttt ctacagcgca gtatctaatt atgttgaagt gacacaagat
    17641 catataaaag ccacagaaaa ctggatagaa aatgatgaaa ccctcagaag acctgcaacc
    17701 gttgctgaag agtttgataa tgtttatgcc agaaagtttt atgactcact tatgcaggga
    17761 atgctcagaa ggctcataaa tgaaaattta aagcatgatc ctgaaaatca tgtgctcctt
    17821 caggcaaagc aggaagctga taagagattt gaagagctta accatttcct tgaagaaaat
    17881 ctaaagtata agaccattcc cataaagagt cttgtatgcg tgcagatgat tgcagggctt
    17941 aatactatgt acagactcca ggaaaaagga gagaagtaat gaaacagtac ttcctgaaaa
    18001 gatttttgga gcttatacct gttttttttc tgatctcatt tataatattt atgctaataa
    18061 accttatgcc cggagatcct ttgcttcaga tgaggatgga caatcccagg gctgtaatga
    18121 acgatcctca aaggatgcag gtactcagag agtactatca ccttgatgat cctcttatag
    18181 tgaggtactt tatctggctt aaaagtgctc ttactggaga ttttggatat tcgagcatgt
    18241 ataaggtgcc ggtgtgggat ctgatagcca accgtcttcc caatactctg atactcacaa
    18301 taggtgcatg gatacttgga ctcataatag cacttcctat aggcatatac tctgcggtga
    18361 agaagtactc tttttttgat tactccacaa ccgtaatggc ttttataggc atatcacttc
    18421 caagcttctg gtttgccctt gtttcaataa tatttttcag cgtaacctta aaatggcttc
    18481 ctgtttccgg aatggagacc tatggaatga caggcacatg gaatgttttt tgggataggt
    18541 taaagcatct tatcctgccc gtatgtgtcc ttggactggt acaggttgcg tcgtgggtaa
    18601 gatatataag aacctcgctt cttgaggtgc ttgaccaaga ttatgtcagg actgcttatg
    18661 caaaaggtgt tccggataaa aaggttattt ttaagcatgc actgaaaaat gccatgatac
    18721 ctatagttac cataattgct cttgatatac cttatttttt tggtggagca ctcataattg
    18781 aaagtatatt ttcatggccg ggcatgggaa ggcttatgta tcaggcggta ataggtagcg
    18841 attataacct tgccataagc tgtctgatgt ttcttacggt tataacgctg atttcgaacc
    18901 ttgccgctga tcttatatat gtccttattg accctaggat tcgagtcggc aaaaaggcat
    18961 gatggtgata tgaatgggat tatctaaact gttctataaa aataaagtaa aagaaattga
    19021 agaaaaagca tctccgataa atatccttac atacagaaag cttgtttgga tgaagtttaa
    19081 aagccataag ctcgcactta taggttcgat tatacttgcg gctctgataa tcttctgctt
    19141 ttttggtcct ctctttacaa acaaaagcta tgaagatatg gattttagtg caattttttc
    19201 tccgccatct atttcaagcg gacatcttct tggaactgac gagttgggac atgatgtgct
    19261 tataaggata atgtacggcg gaaggatctc acttatagta ggatttcttt cagccctgct
    19321 tactactgtt ataggaacca tagtgggatt gctttcaggt ttttacggag gtattgtgga
    19381 tcgtttttta atgaggtttg tcgatataat gctttcaata ccaatgtttc ctatacttct
    19441 tatccttacc atggttttcg gatcaggtct tatgaacatc gtaatggttc tgacggtttt
    19501 cggatggatg agcatttcca ggcttgttag aggccttact cttagcctta aggagtctga
    19561 atatgtaata tctgccaaag cggtgggcgt aaaaaatatg gagattattc ttaaacatat
    19621 ttttccaaat gttcttccga tagttattgt atcggcaaca ctgaacgttt catatgcaat
    19681 tctttcggaa tcatcgctca gttatcttgg acttgggata cagcctccga tgccttcttg
    19741 gggcaatatg ctccagaagt ccatgaacta tattctcggg acagtatccg ggacaacccc
    19801 gtggtggctt acttttttcc cgggactttt catatttctt gctgtcttaa atgtaaattt
    19861 cctcggcgat gggttacgtg atgcgcttga tccgaaattt acaagcaata gttgagaggg
    19921 ttgtcaatat gagcgaaaaa atacttgaag tgaaaaatct tgaaataagt tttaaaacac
    19981 agtttgggtt ggtaaaaccc gttgatgatg tgaactttca tataaacaag ggggagagtc
    20041 ttggtattgt cggagagtcg ggctgcggaa aaacagtcac cgcattttcg ataataaggc
    20101 ttcttccaaa aaatgctgtt ttgggtcctg ccacaagtat tatgttcaac ggaaacgata
    20161 tctccaaata ctccaaggag gagctacaaa aagtccgcgg caaaagcatc tcaatgatat
    20221 tccaggaacc catgacatcg cttaatcctc ttttcaccat agggcagcag ctttcggaag
    20281 tttttatgct ccatgaagct cttaatgaga aacaagctat gcaaaaaagt ttggagatac
    20341 ttgagatggt caggattcct gaagctaaaa accgtatgga aagctacccg catatgctgt
    20401 ccggaggaat gcgccaaagg gtaatgatag caatggcact cgcatgcaag ccttctcttt
    20461 tgattgccga cgagcctact acagcattgg atgttacaat acaagcccag atacttgagc
    20521 tcataaacga attgaaaact attcaaaata cggcatccat gataataacc catgacctag
    20581 gcgtaattgc cgagatgtgc gatagagtta tcgtaatgta tgcaggtcaa attgtcgaaa
    20641 ctgccgatgt atacagtatt tttgacaacc ccaagcatcc ttacacacag gggcttctta
    20701 actctatacc tgttcttgaa atagcgaaaa aagatcagaa aaaacttcac gtaataaaag
    20761 ggtatgttcc tcatccatcg gcattcccaa aaggatgcag attcaacccc aggtgcgagt
    20821 ttgcaagtga aaaatgtatt tcttaccagc cacataactt taaaataagc gatacccata
    20881 cagtaaaatg ttggctttat gaaaactctg agcaaggtga tgcagatgtc tgaaaaaaaa
    20941 gaaatactta atgtaaaaaa tttgaagaaa tggtttcaga tgaaaggaca gatgttttca
    21001 aacaaaaaat cctttcttaa agcagttgat gatgtctctt tttcactgaa tgccggagaa
    21061 actcttggta tagttggaga atcaggatgt ggaaaaacta cggttggaag aaccttgatg
    21121 agaatatatc agccaacttg cggagagttt tggtatgatg aaaagcttac cggacagccc
    21181 gtagatatat ttaagcttaa ggatagacag atgcagaaaa taagaagcca tatccagatg
    21241 gtctttcagg atccttatgc ttcgcttaat ccgagaatga cagtacagga tataatatca
    21301 gaaggtctca gggtaaataa actcacttct tctaaaaaag aaaccaacga gagaatagcg
    21361 gatataatgg aaaaagttgg gttaagacca gagtatatga agcgttatcc ccatgaattt
    21421 tcaggaggtc aaaggcaaag aataggcata gccagagtta tgatcatgaa acccaaagtt
    21481 gtaatctgtg atgaacctgt ttctgcattg gatgtttcag tccaggcaca gataatcaat
    21541 ctccttgaag atctgaagca tgaatttgat ctgacatatc tgtttatagc ccatgatctt
    21601 tcagtggtaa agcatatttc agacaagata gccgtcatgt atcttggcaa ggtagtcgaa
    21661 agcgcctaca cagacacact tttcgaaaat tcacttcatc catataccca gggtcttttg
    21721 catgccattc caattcctaa tccgcatacg cgcagaatgg gaaaaagaat actgataacg
    21781 ggagatattc cttcgcctgt agatccgccc gaaggatgca ggtttgcaaa aagatgtcct
    21841 aaggcttttg acagatgttt tagagaaaca ccagtactta cagaaaagga aaaaaaccac
    21901 aatgtagcat gttttttgta ctaagtactt tactcctact cttcctctag tgcacctgta
    21961 aattgacagg tgcagttttt tttatacatt gtgacccatt tatgccaaaa ttctgtgtaa
    22021 tagtaaagtt tattttatcc ggaaaaatat gttatcatat agaggatcaa tcattacagt
    22081 attatattgg aggtaagggg agatgaaatt gaaacttggt caaaagattg tattaattct
    22141 gacggtaaca caaatagcag catttttcac ggttttactt tatcttggaa tcacatttac
    22201 agatattctt aacaagcaga ctggaaatgc aacgttgaat gctcagcaaa ttttgttaaa
    22261 acccataata tgggcttttg ttatagcctt gttgtggatt gccgcttcgg tatttttcac
    22321 ttatagggca ataataaaag gggtaagtct tcctttaaaa tatctatcat ctcaggtagt
    22381 agatttcgga aacggaaacc taaaagtgaa gttcaggcag aaaacacagg attcagtcgg
    22441 gcagatggta ggggctctca ggagcatgac cgaaaatctt catggtaata tgataaaaat
    22501 aaaagaaatc ggagaacagc tcaatgaatc gtcggaaaag cttcaaaatg caagtgaaag
    22561 ctctgaaaag catgttacaa aacttcaggg agatatttct gattttactg ccggcataag
    22621 cgaatccacg gagagtataa tagacataaa cacatccata gacagaattt caaaaagctc
    22681 tctcactata tcaaacgact cgcaaaatct tt
//
LOCUS       DAOSHR010000008        21627 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_1958_length_21627_cov_5.629520, whole genome shotgun sequence.
ACCESSION   DAOSHR010000008 DAOSHR010000000
VERSION     DAOSHR010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 21627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21627
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1958_length_21627_cov_5.629520"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(16..1014)
                     /gene="purM"
                     /locus_tag="PLU28_00870"
     CDS             complement(16..1014)
                     /gene="purM"
                     /locus_tag="PLU28_00870"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HOG33646.1"
                     /translation="MKDYKESGVDINEGYRTVELIRKHTKRTMIPGVLNDIGGFAGMF
                     ELKNYKNPILVSGTDGVGTKLDVAMKMKKYDTVGIDCVAMCVNDVLCNGAQPLFFLDY
                     LACGKLDAEIAADLVKGVADGCVQAGCALIGGETAEMPGFYDIGKYDIAGFSVGVVEK
                     DKIIDGSTIRQGDTLIGIQSSGPHSNGFSLIRYLIKDFDMPFDNRKIGEVLLTPTIIY
                     VKVIHKVLEKYSIKGMAHITGGGFYENIPRMFATKEYTAVIKKESYEVPKIFKYIQSL
                     GVSEKDMYNTFNMGIGFVICVGEDDAQGVMDIIKESGLDSYRLGYVKKGEYGICLE"
     gene            complement(1011..2414)
                     /gene="purF"
                     /locus_tag="PLU28_00875"
     CDS             complement(1011..2414)
                     /gene="purF"
                     /locus_tag="PLU28_00875"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOG33647.1"
                     /translation="MYPDKLHEECGVFGIFSNGKEDVASITYYGLYALQHRGQESAGI
                     VVSDEKELLCHKGMGLVSEIFSEDDLKKLKGKNAIGHVRYSTAGSSNINNAQPILVKC
                     KIGSVAIAHNGNLVNAEILKELLEETGTVFQTTTDSEVILNLIARSSRHGLEKAITDT
                     VFAIKGSYAIVMLTEDKLIGIRDPNGIRPLCIGKLKDDYILCSESCAIDAVGGEFIRD
                     VEPAEIVMIDKEGIRSFKNSEKIKSETCAFEYIYFARPDSIIDGIDVYQARRRCGHVL
                     YEESPISCDLITGVPDSGVAAAIGYSEASGIPYGMTLIKNKYIGRTFIAPSQKQRQNS
                     VNVKLNALKSNIKGKRILMIDDSIVRGTTSKKLVTLLRNAGAKEVHMMIASPPVKFPC
                     YFGIDTPERGQLIGATMSIEEIRKEIGADTLAYLSVPGLLRALDHGNTFCLGCFNGIY
                     PVEAPIEKSKLYLEDKK"
     gene            complement(2416..3135)
                     /locus_tag="PLU28_00880"
     CDS             complement(2416..3135)
                     /locus_tag="PLU28_00880"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002867669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOG33648.1"
                     /translation="MEKLEMIYEGKAKKVYATNSKDYVIIHYKDDATAFNGEKKGQIT
                     DKGIFNNEITSIIFGLLNSKGIPTHFESKINEREQLCKKVQIVPLEVITRNVAAGSMS
                     KRLGIAEGTELKTTVYELSYKNDDLGDPLINDYHAVGMGLTTFDELRVIYDLTQKINE
                     ILKDFFIKMGIKLIDFKLEFGRFNGHIVLADEISPDTCRFWDSKTNEKLDKDRFRRDL
                     GNVREAYAEILKRLENNNKEG"
     gene            complement(3135..3614)
                     /gene="purE"
                     /locus_tag="PLU28_00885"
     CDS             complement(3135..3614)
                     /gene="purE"
                     /locus_tag="PLU28_00885"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HOG33649.1"
                     /translation="MKVAIIFGSSSDTQLMQPAAALLKEFGIEYKAMVLSAHRVPEKL
                     SQEIKKLEENGFECIIAGAGLAAHLPGVIASQTIIPVIGVPLNAALNGLDSLFSVVQM
                     PKSIPVATVGINNSYNAAMLAIEILSVKYPDLKDKLISYRKNMKEKFIKENSEGVNM"
     regulatory      complement(3685..3786)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(3812..7567)
                     /locus_tag="PLU28_00890"
     CDS             complement(3812..7567)
                     /locus_tag="PLU28_00890"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HOG33650.1"
                     /translation="MSDKIYRVYVERKKGFDLQANGVLNDLKNYLGITSLKTLRILNR
                     YDILGIDEGTAERALKEVFCEPQTDDLYLEDFPMSSFEKSFGIEYLPGQYDQRADSTQ
                     QCLQLINPLLKPKVKTAVIYVVNNDILPSELEKITSYLVNPVDSRLANLGKPDKFDEQ
                     IQHDINIEYLHGFSNFSRLQMEELYNKLSLAMSFEDFLFCQEYFQKEKRDPSMTEIKV
                     IDTYWSDHCRHTTFNTIIEKIDWDDRTPGYIKKAYEQYLKIREEVYKDSEKPITLMDL
                     ATVFPKKMRKDGKINDLEVSEEINACSVEVDVSCKNGTEKYLLMFKNETHNHPTEIEP
                     FGGAATCLGGAIRDPLSGRAYVYQAMRVTGAADPRTPVEKSIKGKLPQRKITTLAASG
                     YSSYGNQIGLATGIVNEHYDDDFVAKRMEIGAVIGAVKKSDVTRKTLGVGDIIVLLGG
                     ETGRDGCGGATGSSKKHTTESINTCAAEVQKGNPVTERKIQRLFRNPELTKIIKKCND
                     FGAGGVSVAIGELADGLNINLDLIPKKYEGLTATELAISESQERMAVGVDPKDLEKFL
                     ELARIENLQATAVATVTNDARLRMSFKGQTVVDISRDFLNSNGVRQKISISVPATYLS
                     ENKKVSETFTTGEKIKRLLSDLNVCSKKGLIERFDSSIGSSTVLMPFGGKYQLSPVQA
                     MVAKIPVIDDSTDTVSIMTYGYDAALAKEDPFKGALYAIVHAAAKTVACGGRYEKIRL
                     SLQEYFERLEKDSHKWSKPFTALLGAFYAQNALQIPAIGGKDSMSGTFNDINVPPTVV
                     AFAVSTENSKNIISTDFKKTSTLVALIKTEKDNDGLVDFQVLKDNFDRVSKMIRHGKV
                     YSAYAPGYAGTVEAVLMMAFGNKIGVDFTCCKDFDSLFIKDYGSILIEVSSEDDLKEF
                     FDNGHWEIVAKTSPVSKVKGKDFEFDLQELIDTWEKPLSTVFPNNIECKESIESIQER
                     IFEKKFLCRTKTISPKVIIPVFPGTNCEYDMRKAFVKAGASTQVLVFRNRTSDEINDS
                     IVELAKAIKNGQILALSGGFSAGDEPDGSGKFIASVLRNDIVKQALHYLLDKNDGLIL
                     GICNGFQALIKSGLLPFAHICSMDESSPTLTYNKIGRHVSTIVRTKVVSNLSPWISDG
                     MVGEVFSIPVSHGEGRFVCSTQQYEQLYCNGQITTVYVDLKGEPTLCYPFNPNGSFMA
                     VEGITSPDGRIFGKMAHSERYDEDLYKNIYGNKNQNIFKNGVEYFKL"
     gene            7728..9740
                     /locus_tag="PLU28_00895"
     CDS             7728..9740
                     /locus_tag="PLU28_00895"
                     /inference="COORDINATES: protein motif:HMM:NF019436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG33651.1"
                     /translation="MQIMYFIIKYLGVPAFMVFSFFVLLSGVAAYDIRNIPSGIEILH
                     SADNEVTVKWKDNNPHEFGYQFLLKNLSNNQIKTYNLPTDSSSFRFEIQKNNLYSFQI
                     LSNDFNSYKSQEHRFYYADLPEEDVIFECCDNKIGIPKEFFDLTTKIFLFSEYRKTDT
                     PDIKIQLDNEVLSHQKPFPKFSIPIMLFHPKGEVINISKNIFIKRTGDTYENSLAYRT
                     EELTAVIPPQLTNKEYSYIFPLLFDFDILNRIELVISNSAYKSIEGYAELFRSSVQEN
                     IRREGLSFIDTNRTISKLSSSDKYKDCALIFVHGHQIETLDKSFNISSRSNPWINNDR
                     IDVWRDFFKEIRASEDFKNTFDFYEFIYDTHALSAKQYGEMLASYITQLKKSGNYKDI
                     FIIAHSMGGLVSRYAMNTVVSGEEEYLGAYVTKVYTLDAVNRGSPLQSVVRSFDDRLL
                     RQGLINYPMVYDYLAELVKSFPSMSMDFNYANLFYDDVFVQRVSFLLTQALKYYPNFF
                     AVFVSQNLLGIFNGPLSFEYSDGEYLRKFQNYFYPGIEEVLFVPNTALNSLINDERFT
                     EKYCEVISNIAYPLTDNKPIDPTHYSEYVHLGMRLFNDVLADVAAEINMPEHSLSDGM
                     VTVWGQTVKNDERTKLRFIGLDHAQVYYNHEVITSILKDIAIRTLK"
     gene            9737..10564
                     /locus_tag="PLU28_00900"
     CDS             9737..10564
                     /locus_tag="PLU28_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG33652.1"
                     /translation="MNIFFICALRAEAKPFIEALNLKKLLDQKAFDVYKRNNFFLCIS
                     GIGKVKAAAATAYVLTAFSKNAGDILINCGVAGYNTPLISKGETVLINKITDQDTFKE
                     FFPNIIISHSYKEHEIHTFSKAVSLSSPEKKEFVDMESSGVYIAASRFLYLNNIYFLK
                     VALDNLSPEGIKEESILSVMSENVNSILNFAESADLLSQNTSFEFNFSQEDERKLSEL
                     FEKMKLTKSMKDKVMLLLKKRYVCGKKTLPDMQNIIFPVTNKDERKKEFDKLIEYLS"
     gene            10536..11552
                     /locus_tag="PLU28_00905"
     CDS             10536..11552
                     /locus_tag="PLU28_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG33653.1"
                     /translation="MTSLSNIYLKKPFSHIYVENQAFKYDETYRITNFFKNSQLIEVS
                     DYRNVFNSHNQNFNLQKKTPDLILAVKKGDFLYKGSDLCHGYENMDFYYLTNIINCLY
                     DCDYCFIRGMYTSANIVYFVNTDDFFDAVAHEALGKDIFIPISYKSDLLVMESIFRIF
                     ARWHSFCVENSNVNIEVRTKSSCINIIKEYRPVRNILLSWTLSPDLIISSYEKKSPPL
                     QKRIKAVNQALEAGWKVRLSFDPILLIDDAEKIYTSFFNQILSEIPIDFLFDLNIGFF
                     RMPKTYFREYKSKTQFSPVLYSDWEYSADHVTYSSDRKEKFMKSAEPYLKEFSRLTKV
                     LYMV"
     gene            11584..12042
                     /locus_tag="PLU28_00910"
     CDS             11584..12042
                     /locus_tag="PLU28_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG33654.1"
                     /translation="MKKTVFIFLVLLIFSTAVFSGELLKITTLSPNLDNALAHIGVSF
                     LTDYNVSAGIEFPLIRAELFDSFSILGLYGFSFTSARPVIGPYFEAQFLVGKFKMFLG
                     TLMNPDIILLGNPVLMKTGVLAGALGGYVKLGIDFDSKFSKALLYMAIEY"
     gene            complement(12083..13009)
                     /locus_tag="PLU28_00915"
     CDS             complement(12083..13009)
                     /locus_tag="PLU28_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HOG33655.1"
                     /translation="MIRIYEKANGKLVESQGFSQDCLIYLVSPTSTEIDDISQKLSID
                     SDLIYDSLDPDERARIDISGKSTLLILKVPYRDEDDKKIPYKTASLGILIGSDYTLLI
                     SKFNLEFLDIMIKRSELNPHKKSRLVFQIFYRNALLFLAYLKEINKITDRTEERLHES
                     MRNNELETLMYLEKSLVYFTTSLRSNEIMMEKILRGTTIEIFDADKELLEDTIIEYKQ
                     ALEMANIYSDILSGMMDAFASVISNNLNVVMKVLTILTLVIQIPAIITGFFGMNVELP
                     FSDNPYAFIYIISVCVVVCILVYFIFRNKKWM"
     gene            13194..14666
                     /locus_tag="PLU28_00920"
     CDS             13194..14666
                     /locus_tag="PLU28_00920"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOG33656.1"
                     /translation="MQSQNETNIFGRISNIIMELSKIGDIESSYSTLSSLINSMVKTE
                     EEYFFAEKESTLSEIFNHNYSRDIYDLIKWGYSQGDISMFPEGEGSIIILPISKGPKV
                     LFVYAAYVKLQEFSNEFMMFLRIIAFLMGSLYENLLLYNEILDKNAIIEKNKNFLDTL
                     INGSIDSIIVYDKSMNIYFKNSTYDSISENSLLLEKLHDINIATIDSNKRQIVELEQD
                     GKFYSIESLPIYFEQESFALCFIRDISGTKELEKLKQLDKMKMEFLSMMSHELRTPLS
                     AVKAYSETIITAFEDMDKDTVLSFLTTILTEANHLEKLLNDLLDFSKLELKTLKLEPS
                     VFDIVELINDIINSSEQPATAPKVTVSFEKSKEHIKVNLDKSRVRQAITNILQNAIKF
                     CDREKECCFAKISLDDTPSSVEIKVEDNGIGINEKYFSKVFEKFFKVDSSLTYQVQGT
                     GIGLSMAKEFVEMHGGSICIEKSTEGIGTTFKITLPKGVL"
     gene            14666..15568
                     /locus_tag="PLU28_00925"
     CDS             14666..15568
                     /locus_tag="PLU28_00925"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOG33657.1"
                     /translation="MADISIKEITRKIEELPTPDFIVQRIVSIASSPNASTKELNMAI
                     LESPSMAAKILKMANSAYYAMPRRITKLSQAINILGFRSVRNLALSVFTAKSFFSREF
                     EYFNTKKVWSHFMATAISCELLSKFINFPDREEAFLCGMLHDLGKMAMSIVMPDVFEM
                     IVTVAKHKKISFSKAEELLETYSHQQIGRMLFENWQMSEIMIASATFHDSPSLCKSED
                     SRTIVNLVSIANTTVNILLYGYSGCFDIPAVSDEAWNNLGLTPRKYLNYFENLKIKIK
                     ESEDFMNLSNVLDDLQEGELDGTV"
     gene            15555..15923
                     /gene="rbfA"
                     /locus_tag="PLU28_00930"
     CDS             15555..15923
                     /gene="rbfA"
                     /locus_tag="PLU28_00930"
                     /inference="COORDINATES: protein motif:HMM:NF014130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOG33658.1"
                     /translation="MAQFKKQMLESEIEKIITVSLRDIKDPRVCGKILTINRVDMKKD
                     KSLVDIYISCLDNSDLKETVDVLNRAKGFFRSMIAQNINIFKVPDVAFHENRGIEQSV
                     KIHKLLDDIKQMDEKKGKKL"
     gene            15920..16828
                     /gene="truB"
                     /locus_tag="PLU28_00935"
     CDS             15920..16828
                     /gene="truB"
                     /locus_tag="PLU28_00935"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOG33659.1"
                     /translation="MNDGIVIVDKKEGITSHEVVSYFRKKFDTPKVGHSGTLDPFATG
                     VMVVGINRGTKILHFLSAQRKNYHVTAELGKITDTFDISGKVKEINEVTNENLLNLES
                     VVMSFIGKYKQTTPAYSARRYQGKRLYEYARQGKIISLPIKEVEIFSIEELMQNGKEF
                     SFTVSVSSGTYIRSLIMDIGYKLGCGAVTTKLRRLKSGSFDISKSKKMEDISFSDIIS
                     IENSLDFPKIHVFDGTKVINGVQIFKNDIMSYDEFTKGSYIRLFDENNEFLGLGVAEK
                     NSSFMPFLLNSTDRNDRVVKIFRIMR"
     gene            16832..17728
                     /gene="ribF"
                     /locus_tag="PLU28_00940"
     CDS             16832..17728
                     /gene="ribF"
                     /locus_tag="PLU28_00940"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HOG33660.1"
                     /translation="MYALAIGTFDGIHKGHQYILHQTQKVAQENNLKPLAYMIRYPID
                     KYRGNFSGLILPSWRRKELVEDMGFEVEIFDMQDLWDITHIDYIDILLNKGVKHIVCG
                     RDFTFGKGKLGNVDYILNQATPKGLGLTVLQDLKEYGSRVSSTLIRKALTDGDIDLAN
                     EMLTRSWSVEGRVYEDRHVGFKLGFPTANIDFREKEFVISPKYGVYLVKSSLPGYSRT
                     FWGLMNVGLRPTYRENLNGPKVEVYFMDFFGDLYGKHLKVEVIKYLREEMMFGSEKDL
                     ISQMIKDEDTARKLVITMEKGA"
     gene            complement(17738..18982)
                     /locus_tag="PLU28_00945"
     CDS             complement(17738..18982)
                     /locus_tag="PLU28_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG33661.1"
                     /translation="MKKLFIALFVIMTGVVMLAGQQRYTFVFRDALVTYLQYDESRFS
                     IPDNFNLLWVSGAKSWYVDEENTAFPFKLVDINDFSQFSLKEIYSNVFEIEGRQDILF
                     YNSRISKWCLTDKKNLDSIRSKRTLILDAQGKTFIGLYASGNWKNTYEIDNMGNFISK
                     LEINGVPVGETRLYLVNSSVNISSGYYQQEALLRTKSIAYAADSSNSYGAQVQSDTLF
                     FDLGMVDMNSEYMALTADRYKLASFEDRYTVNPNLYSNITDPQPASIIRYFDNTVSNG
                     IGKDLASGEVWLMENFDENTFAIKKGYIRDTPKDSEAWINLKSSWSLKYKSTITKDVT
                     YNTTGGKDKRIMQVKIEFQNASSETYKTRIYLSQPGITLDSFTNKGVASKIDNKSQGG
                     IVWLDFDVSKDCYIELNLSVEK"
     gene            19122..20279
                     /locus_tag="PLU28_00950"
     CDS             19122..20279
                     /locus_tag="PLU28_00950"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG33662.1"
                     /translation="MNFLVFFVSIAHFTVDFFADIFQPLIPFFMQKYAITDQPKTLVL
                     MASVLSMTASMSQLIIGYYTDRSSKKLILIYFFVVFSIFPGFVMGYAPNLTYLFFIFL
                     IGYIANAAQHPLGASIAGHVGKGKSLTLAFYVLGGTFGSGLGPIVITWYVKNFGIENT
                     WAIGLIMFVMFSILTLYAYKYLKNNEILKPSSNTASLLQVIKSLKVLYPIWILTVCRS
                     FISSLFHMYGPIRTNSMGYDLTVGGLMLSSALISGMFSNMLGARIYERHGIKKLNVLS
                     FSGYTVFLLMFALSKNMIFIALSFVLADIFMFFTMSANVSYAQKILPQFRGVASGALM
                     GFAWACGNLIVMAFSSVFGNNIEFMINSLWFFAAIGIILTFILFRKKTVLT"
     gene            20324..20965
                     /locus_tag="PLU28_00955"
     CDS             20324..20965
                     /locus_tag="PLU28_00955"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HOG33663.1"
                     /translation="MRTELYTWKYMELGEKVVSTLKEKRHDAYLVEKKEDVIQKVKDL
                     IPSGSKVAVGGSITLNECGILELLRNRDYEFYDRYSAKNHEESIRINRQAFETDFFLC
                     SANAITLDGKIVQLDGTGTRVAPMIWGPRNVIIVAGMNKIVSSIDTALERIRFISPMN
                     AKRLNIKTPCTRIGECINCESSERICVHQVIVETGVRTPGRFKIILVAQDLGL"
     gene            complement(20928..21239)
                     /locus_tag="PLU28_00960"
     CDS             complement(20928..21239)
                     /locus_tag="PLU28_00960"
                     /inference="COORDINATES: protein motif:HMM:NF013479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HOG33664.1"
                     /translation="MRDDKKKAVALRYKKGEDTAPKVIAKGIGEIAERIIEVARKEKI
                     AIIEDKAAADKFYAVDVNVEIPPEMYKVAAELLAFVYTLDKKGRTGGVKAPGPVPPKL
                     S"
     gene            complement(21254..>21627)
                     /locus_tag="PLU28_00965"
     CDS             complement(21254..>21627)
                     /locus_tag="PLU28_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG33665.1"
                     /translation="QVRLNKKNKTIAFTSLPMYESPGQYYFDKGEYNIFLTHVPANIY
                     KFSSFIDNADLILAGHTHGGQINLPFLTRLLLQSHFSKESFIKGMYNEQNYDIYVNSG
                     IGSWFNARLFCPPEITVFEID"
BASE COUNT         6634 a         4105 c         3753 g         7135 t
ORIGIN      
        1 ccgaaatgag aacagctatt ctaagcatat tccatattcg ccctttttca catatcccag
       61 gcggtatgaa tctagcccgc tttctttgat tatatccatt actccctgtg catcatcttc
      121 tccaacgcat atgacaaatc ctatgcccat gttaaaagta ttgtacatat ctttctctga
      181 aactcccaaa gactgtatgt acttaaatat tttgggaacc tcatagcttt ctttttttat
      241 tacagcggta tattcctttg tagcaaacat tctgggaata ttttcataaa atcctccgcc
      301 agtaatatgt gccattcctt ttatagaata cttttcaagc actttatgaa ttacttttac
      361 atatataata gtcggagtaa gaagaacctc acctattttt ctgttatcaa aaggcatatc
      421 aaaatctttt ataagatatc ttataagtga aaagccatta ctatgggggc ctgacgattg
      481 gattcctata agtgtatctc cctgtctaat agtacttccg tctattatct tgtctttttc
      541 aactactccg actgaaaaac ctgctatatc atactttccg atatcataaa acccaggcat
      601 ttcagcagtt tctccgccta ttagtgcaca tccggcttga acgcagccgt ctgcaactcc
      661 tttaaccaga tctgctgcaa tttctgcatc aagtttccca catgccagat aatccagaaa
      721 aaacagtggc tgtgctccgt tacacaaaac atcattgacg cacatggcaa cacagtctat
      781 cccaaccgta tcatacttct tcatcttcat ggcaacatca agcttggtac cgactccgtc
      841 agttccggag accagaatcg gatttttgta gttctttaat tcaaacatac cggcaaatcc
      901 gcctatatca ttgagtacac ccggaatcat cgttcttttg gtgtgcttcc tgataagctc
      961 aaccgtcctg tacccttcgt ttatatcaac acctgactct ttgtagtctt tcatttttta
     1021 tcctccagat atagttttga tttttctata ggtgcttcaa ccggatatat tccgttgaaa
     1081 catcccaaac aaaaagtatt tccatgatct aaggctctga gaagtcctgg aacacttaaa
     1141 taagctagtg tatcggcgcc tatttctttt cttatttcct cgatgctcat ggttgcacct
     1201 atgagctgcc cacgttccgg agtatctata ccaaagtagc acgggaactt caccggaggg
     1261 gaagcaatca tcatatgaac ctcttttgca ccggcatttc taagaagcgt aaccagtttc
     1321 ttgcttgtgg ttccccttac aatcgaatca tcgatcataa gtattctttt gccttttata
     1381 ttggatttta atgcattaag ttttacatta actgaattct gtcgttgttt ctggcttgga
     1441 gcaatgaaag ttctgccgat gtacttgttc tttattaaag tcatgccata cggtattcct
     1501 gatgcctccg aatatcctat tgctgcagct acaccggaat caggaactcc tgtgataaga
     1561 tcgcagctga ttggggactc ctcgtaaaga acatgtccgc atcttcttct tgcctgataa
     1621 acatcaattc catcaatgat actgtctggt ctggcaaagt atatatattc aaaagcacat
     1681 gtctccgatt ttattttttc ggagtttttg aatgacctga taccttcttt atctatcatt
     1741 actatttccg caggctcaac atctcttata aactcgcctc ccacagcatc aatagcacag
     1801 ctttcagaac acaaaatata gtcgtctttg agcttgccta tacacagagg ccttattccg
     1861 tttggatcgc gaattcctat cagcttgtct tcggtcaaca tgactatggc gtacgaccct
     1921 tttattgcaa aaacagtatc tgttattgct ttttcaaggc catgcctgga acttcgtgca
     1981 ataagattaa gtataacttc tgaatctgtt gttgtctgaa acacagtacc agtttcctca
     2041 agcagctcct taagaatctc ggcattcact agatttccgt tatgagctat ggccacggaa
     2101 cctatcttgc attttacaag tataggctgt gcattattta tgttgcttga gcctgctgtg
     2161 gagtatctta catgccctat tgcatttttt cctttaagtt ttttcagatc atcttcgctg
     2221 aatatttcac tgaccagtcc cattcctttg tggcataaaa gttctttttc atcgctgaca
     2281 actattccag cactttcctg tcctctatgc tgaagagcat acagaccgta atacgttatg
     2341 gaagcaacat cttcctttcc gttggagaaa ataccgaata ccccgcattc ttcatgcaat
     2401 ttatctggat acatttcagc cttccttatt attgttttca agtcttttta atatttccgc
     2461 ataagcttct cttacgttgc caagatctct tctgaatctg tccttatcta atttttcatt
     2521 tgtcttgcta tcccagaacc tacaggtatc tggagaaatt tcgtctgcaa gaacaatatg
     2581 tccattgaat cttccgaact ccagtttaaa atctatcagt ttgattccca tttttataaa
     2641 aaaatcttta agaatctcat ttatcttctg agtaagatca taaatgactc taagttcatc
     2701 aaacgttgta agacccattc ctactgcatg ataatcgtta ataagcggat cgcccagatc
     2761 atcgttttta tatgaaagct catacactgt ggtttttagt tctgtgcctt cagctattcc
     2821 aagtcgtttg gacatgctgc ctgcagcaac attcctcgta attacctcaa gaggtactat
     2881 ctgaactttt ttacagagct gttctctttc gttgatttta ctttcaaaat gtgtcggaat
     2941 acctttgctg ttaagaagac caaatattat ggaagtaatc tcattattga atattccttt
     3001 atctgttatc tgccctttct tttctccgtt aaaagccgtt gcatcatctt tatagtgaat
     3061 aataacataa tccttcgaat tggttgcata gacctttttt gcttttcctt catagatcat
     3121 ttctaatttt tccatcacat gttcacccct tcagaatttt cttttataaa tttttccttc
     3181 atatttttcc tgtagctaat aagtttatct ttaagatcgg gatactttac agaaagtatc
     3241 tcaatagcaa gcatcgcagc attatagctg ttatttattc ccactgttgc gacagggatg
     3301 gacttaggca tctgcacaac tgaaaaaagc gaatccagtc cattaagtgc tgcgttaaga
     3361 ggaacaccga taacggggat aatggtttga gacgcgataa ctcccggaag atgggccgcc
     3421 agtccggctc cagcaattat acattcaaag ccgttttcct caagtttctt tatttcctgc
     3481 gatagttttt ctggtaccct gtgagctgaa agaaccatgg ccttgtactc tattccaaac
     3541 tctttaagaa gtgccgctgc aggttgcata agctgagtgt ctgatgaact tccgaaaata
     3601 attgctactt tcattttccc tccaaataaa attccgcctt atccaggaat cagaataaag
     3661 gcggaataat tttttcaaat gcaattccac atttttctga tccatagcag agaaatttac
     3721 ggcttctccg tagagactcc caaaccatat cttcgggatt atatggatct gttgtattca
     3781 cttttatata gtaattctat catatatctt tttataattt gaagtattca actccatttt
     3841 tgaaaatatt ctgatttttg ttaccatata tattcttata cagatcctca tcatatcttt
     3901 cagaatgagc catttttcca aaaattcttc cgtcaggact agttattcct tcaacagcca
     3961 taaaagaccc gtttggatta aacggatagc aaagagtagg ctcacctttg agatctacat
     4021 aaacagttgt tatctgtcca ttacagtaca actgttcata ttgttgagtg gaacatacaa
     4081 atcttccctc accatgagaa actgggatac tgaatacttc accaaccatg ccgtctgata
     4141 tccacggtga cagatttgaa acaactttgg ttctgacaat cgtggaaacg tgccttccaa
     4201 ttttattgta agtaagcgtg gggctgcttt catccatgga gcaaatatgc gcaaaaggaa
     4261 gaagtccgct tttgataagc gcctgaaaac cattacagat accaaggata agtccgtcgt
     4321 tcttgtcaag aagataatgc aaggcctgtt ttacaatatc gttgcgtaaa acacttgcaa
     4381 taaactttcc ggatccgtcc ggctcgtcgc ctgcgctgaa tcctccggac agagccaata
     4441 tttgaccatt ttttatagct tttgccagtt caacaataga atcattgatc tcatctgaag
     4501 tcctgtttct gaatacaagt acttgagtag acgcaccggc tttgacaaaa gcttttctca
     4561 tatcatactc acagtttgtt cccggaaata ccggaattat aactttagga gaaattgttt
     4621 tggttctgca taaaaatttt ttctcaaaaa ttctttcttg aattgattct atactttctt
     4681 tacactcaat attattaggg aaaacagtac taaggggttt ttcccacgta tctataagct
     4741 cttgaagatc aaattcaaaa tcctttcctt taactttaga aaccggtgaa gtcttggcta
     4801 caatctccca atgtccatta tcaaagaact ctttaagatc atcttcactt gatacttcta
     4861 taagaattga accataatct tttatgaata atgaatcaaa atccttacag caagtaaagt
     4921 ccacaccaat cttattgccg aaagccatca tcagaacagc ttcaactgtt cctgcataac
     4981 caggcgcata tgccgaataa acttttccat gtcttatcat tttacttact ctgtcaaaat
     5041 tatctttaag aacttgaaaa tcaacaagtc catcattatc tttttcagtt tttataagtg
     5101 caaccagagt tgatgttttt ttgaaatctg ttgaaataat gtttttggag ttttcagtac
     5161 ttactgcaaa ggcaactacg gtaggaggaa catttatatc attgaaagta ccagacatac
     5221 tgtcttttcc acctattgcc ggaatctgaa gagcgttctg ggcataaaat gctcccaata
     5281 gtgcggtaaa aggtttagac catttatggc tgtctttttc aagacgttca aagtactcct
     5341 gaagagaaag tcttattttt tcatatctgc caccacatgc tactgttttg gcagccgcat
     5401 ggacaatagc atataaggct cctttgaatg ggtcttcttt tgcgagcgct gcgtcgtagc
     5461 cataggtcat tatactgacc gtatcggtac tgtcatcaat gacaggaatt tttgcaacca
     5521 tagcctgaac cggcgaaagc tggtatttcc ctccgaaagg cattaacact gttgaagatc
     5581 ctattgaact gtcgaatctt tctataagtc ctttcttgga acatacattc agatcagaaa
     5641 ggagtctttt tatcttttca cctgtcgtaa atgtctcaga tactttttta ttctctgata
     5701 agtaagtcgc gggaacagaa atactaatct tttgccttac tccattggaa ttaagaaaat
     5761 cacgggaaat atcaacaaca gtttgccctt taaagctcat tctaagcctt gcgtcgttag
     5821 ttacagttgc aacagccgta gcctggagat tttcgatccg agcaagctcc aaaaactttt
     5881 caagatcttt tggatcaaca ccaaccgcca tcctttcctg cgactctgat attgctagtt
     5941 cagttgctgt aagaccctcg tatttttttg gtattaggtc aagatttatg ttcaggccat
     6001 cagcaagttc gcctatagca acacttactc ctcccgctcc aaaatcgttg cattttttta
     6061 ttatctttgt aagttctgga tttctaaaca atctttgaat ctttctttca gtaacaggat
     6121 tgcctttttg tacctctgca gcgcacgtgt ttattgattc tgtcgtatgt ttttttgaag
     6181 aaccagtagc tcctccgcaa ccgtctcttc cagtttctcc tccgagaagt acgattatat
     6241 cgccaactcc gagagttttt cttgttacat cagatttttt tacagcccct atgacagctc
     6301 caatttccat tctttttgca acaaaatcat catcataatg ttcatttaca atacctgtgg
     6361 caagtcctat ctgattacca taggaactat atccactggc agctagagtg gttatttttc
     6421 tctgcggaag ttttcctttt atactttttt caaccggagt tctcgggtca gcagctccag
     6481 taactctcat agcctgataa acataagctc ttcctgaaag gggatccctt attgcacctc
     6541 caagacatgt ggcggcacct ccaaagggtt caatttccgt agggtgatta tgagtttcgt
     6601 ttttgaacat taaaagatac ttttcggttc cattcttaca gctaacgtcc acttcaaccg
     6661 aacaggcatt gatctcttcg gaaacctcaa gatcattaat tttcccatct tttctcattt
     6721 tcttgggaaa aacagtagcc agatccatca aagttatagg tttttcgcta tctttataca
     6781 cttcttccct gatttttaaa tactgttcgt atgctttttt tatatatccg ggagttctgt
     6841 cgtcccaatc tatcttctct attatggtat taaatgtagt atgtctgcag tgatcggacc
     6901 agtaagtatc tattactttt atttccgtca ttgatggatc tcttttttct ttttgaaaat
     6961 actcctgaca gaataaaaaa tcttcaaaac tcatagccag agagagctta ttatacagtt
     7021 cctccatctg tagtcttgaa aagtttgaaa atccatgcag atactcaata tttatgtcgt
     7081 gctggatttg ttcatcaaat ttatctggtt ttccgaggtt agccaatctt gagtctacag
     7141 ggttgacaag atatgatgtg attttctcta actctgaagg aaggatatcg ttgttgacaa
     7201 catagataac cgctgtcttt actttaggtt ttagaagggg gtttatcaat tgcagacact
     7261 gttgagtaga gtcggcccgc tgatcatact ggccaggaag atattctatt ccgaaagact
     7321 tttcaaaaga tgacatggga aagtcttcaa ggtataaatc atcggtctga ggttcacaga
     7381 aaacctcttt aagagctctt tctgctgttc cttcatctat gcccagaata tcgtagcggt
     7441 taagaattct gagcgtttta agacttgtta ttccaagata gttttttagg tcatttagta
     7501 ctccattggc ctgaagatca aatccttttt ttctttcaac gtagactctg tatattttgt
     7561 ctgacattat aacctcctga atatgcttta tggtgatatt atatattatt aaaaattgag
     7621 acgatgcttt gaattggtaa caactgggtg aattcaaaat gtgattattt tgttattttg
     7681 atttgtttat tatataatta tataaattag tctttcgagg tgactgcatg cagatcatgt
     7741 attttataat caagtatctg ggggtaccgg catttatggt tttttcgttt tttgttttgt
     7801 tgtccggtgt tgctgcatat gatatacgaa atattccttc cggaatagag attcttcatt
     7861 ctgctgataa cgaggttaca gtaaagtgga aggataacaa ccctcatgaa tttggttatc
     7921 agtttttact taaaaacctt tctaataatc agatcaaaac ttataatctt ccaactgaca
     7981 gttcctcttt cagatttgaa atacagaaaa ataacctcta ctcttttcag attctctcaa
     8041 atgatttcaa cagctataaa agtcaggagc acaggtttta ttatgcagac ctgcctgaag
     8101 aggatgtaat ttttgaatgc tgcgataata aaatcggaat accaaaggaa ttttttgatt
     8161 tgacaactaa aatcttttta ttttcagagt atcgtaaaac agatactcca gatataaaaa
     8221 tacaacttga taatgaagtt ctttcccatc agaaaccgtt tccaaagttt agtataccca
     8281 tcatgctgtt tcatcctaaa ggagaagtaa ttaatatatc aaagaatata tttataaaaa
     8341 gaacaggcga tacttatgaa aattctttgg cttacaggac agaagaactt actgctgtaa
     8401 tcccgccgca gttaaccaat aaagaatact cttatatctt tcctctgctc tttgattttg
     8461 atattctaaa cagaatagaa ctggttattt caaattcagc atataaaagc attgaaggat
     8521 acgccgagct atttcgctca tctgttcagg aaaacatccg aagggaagga cttagcttta
     8581 ttgatacaaa cagaaccatt tcaaagcttt cttcatcaga caaatataag gattgtgctt
     8641 taatatttgt tcatggtcat cagattgaaa ctcttgataa atcttttaat atatcctcca
     8701 gaagtaatcc atggataaac aatgacagaa tcgacgtatg gcgtgatttt ttcaaagaga
     8761 tcagagcttc tgaagacttt aaaaatacct ttgattttta tgaatttatc tacgacacac
     8821 atgctttatc cgcaaagcaa tacggtgaaa tgcttgcatc atacattact cagcttaaaa
     8881 aatccggaaa ttataaggat atctttataa tcgcacatag catgggcgga cttgtcagca
     8941 ggtatgccat gaatactgtt gtatcaggag aagaagagta tcttggtgct tatgtgacaa
     9001 aagtttatac cctagatgca gtaaacaggg gatcaccatt acaaagcgtc gtaagatctt
     9061 tcgatgacag gcttttgcgt cagggactta taaactatcc tatggtttat gattatcttg
     9121 ccgaacttgt aaaatctttt ccaagcatgt ctatggattt taactatgca aatctttttt
     9181 atgatgatgt ttttgttcag agagtttcgt tccttttgac acaggctctt aaatattatc
     9241 cgaacttctt tgctgttttc gtttcacaga accttttggg tatcttcaat ggtccattat
     9301 cttttgaata ttccgatggt gagtatctaa gaaagttcca aaactatttt tatccaggta
     9361 ttgaggaagt tctgtttgtc ccaaacactg ctctaaactc tcttatcaat gatgaaaggt
     9421 ttactgaaaa gtactgtgaa gtaatctcaa atatagctta tccccttacc gataacaaac
     9481 ctatagatcc aactcactac tccgaatatg ttcatcttgg aatgaggctt tttaatgatg
     9541 tgcttgctga tgtagcagca gagatcaaca tgccagagca ctctttaagt gatggaatgg
     9601 taactgtttg gggtcagaca gtcaaaaatg atgaaagaac caagttgagg tttatcggat
     9661 tggatcatgc acaggtatac tataatcatg aagttattac aagtattctt aaagatattg
     9721 cgataaggac actcaaatga atattttttt tatatgtgct ttgagagcag aagcaaaacc
     9781 gttcatagag gctttaaatt taaaaaagct tcttgatcag aaagcatttg atgtatataa
     9841 acgtaataat ttttttcttt gcataagcgg tattggaaaa gtgaaagctg ctgccgcaac
     9901 agcttatgtt cttacggctt tttcaaaaaa cgccggagat attctcataa actgcggagt
     9961 agcaggatac aatacaccgt tgatctcaaa aggagaaacg gttttgataa acaagatcac
    10021 tgatcaggat acttttaaag agtttttccc taatataata atatcgcatt cttataaaga
    10081 acacgagata cacactttct ccaaagcagt aagcctatcg tcaccggaaa aaaaagagtt
    10141 cgtggatatg gaaagctctg gtgtttatat agcagcttca agatttttgt acctgaacaa
    10201 catttatttt cttaaggtag ccttggataa tctcagtcct gaaggaataa aagaagaaag
    10261 tatcctttcg gtgatgagcg aaaacgttaa ttcaattttg aacttcgctg aaagtgctga
    10321 tcttctttct caaaatactt cgtttgaatt caatttttca caggaagacg agagaaaact
    10381 ttcagaacta tttgaaaaaa tgaagcttac aaaatctatg aaggataagg ttatgcttct
    10441 tcttaagaag cggtatgttt gtgggaaaaa aactttacct gatatgcaaa acattatatt
    10501 tccagtaact aacaaagatg aaaggaagaa agagtttgac aagcttatcg aatatctatc
    10561 ttaagaaacc gttttctcat atttatgttg aaaatcaagc atttaaatat gatgaaacat
    10621 accgtataac aaattttttt aaaaattctc aattgatcga agtatctgat tatcggaatg
    10681 tctttaactc gcataatcaa aattttaatc ttcagaaaaa aacgccagat cttattctgg
    10741 cagtaaaaaa aggtgatttt ctctataaag gatcagatct atgccacggt tatgaaaata
    10801 tggactttta ttatcttacc aatataataa actgtcttta cgactgtgat tattgtttca
    10861 ttcggggaat gtatacgtcg gctaatattg tttattttgt aaatacagat gatttttttg
    10921 acgctgttgc tcatgaagcg cttgggaaag atatttttat ccccataagt tataagtcgg
    10981 atctacttgt aatggaaagt attttccgaa tattcgcaag atggcacagt ttctgtgttg
    11041 aaaacagcaa tgtcaacata gaagtcagaa ccaagagttc atgtattaac ataataaaag
    11101 aatacaggcc tgttagaaac attttgctga gttggactct ttctccggat cttattataa
    11161 gttcttatga aaaaaaaagt ccccctctcc aaaaaaggat aaaagccgtt aaccaagctc
    11221 ttgaagccgg atggaaagta agactttcat ttgatcctat acttctgatt gatgatgcag
    11281 aaaaaattta taccagtttt tttaatcaaa tattatctga aatacctata gattttttgt
    11341 ttgatctcaa tatcggtttt ttcagaatgc ccaagaccta cttcagggag tataaaagta
    11401 aaactcaatt ttctcccgta ttatacagtg attgggaata ttccgccgat catgtaacct
    11461 attcatccga tagaaaagaa aagtttatga aatcagctga accatatttg aaagaatttt
    11521 cacgcctaac aaaggtactt tatatggtat aataagctat attcatttga aaaggaggag
    11581 tttatgaaaa agactgtttt tattttttta gtcctgctaa ttttttctac tgcagtattt
    11641 tccggtgaac ttctgaaaat taccacccta tctccgaatc ttgataatgc actggcacat
    11701 atcggagtat cgttccttac cgattataat gtttctgccg gtatagaatt cccgcttata
    11761 cgagcagaac tgtttgacag tttttcaatt ctgggattgt acggtttttc ttttacttct
    11821 gcccgaccgg ttataggtcc ttattttgaa gctcagttcc ttgtgggtaa gtttaagatg
    11881 tttcttggta ctcttatgaa tccagacatc atacttctgg gaaatcctgt attaatgaag
    11941 actggtgttt tagccggagc attggggggt tatgttaaat taggaatcga ttttgacagt
    12001 aaattttcta aagctctttt atatatggct atagaatatt aaataatatc ctgctttggt
    12061 ttaatccaaa gcaggatatt tattacatcc actttttgtt tctgaagata aagtatacca
    12121 agatacacac gacaacacat accgatatta tgtaaataaa agcataagga ttgtcagaaa
    12181 aaggaagttc gacgttcatt ccaaaaaaac cagtgattat ggccggtatc tgaataacca
    12241 atgtaagtat tgtaagaacc ttcataacaa cattcagatt attggatatc actgaggcaa
    12301 aagcatccat catgcctgaa agtatgtcac tgtatatatt tgccatttcc aaagcctgtt
    12361 tatactctat tattgtatct tcaagcagtt ctttgtcagc atcgaatatt tctatcgtgg
    12421 ttcctctaag aattttttcc atcattattt cattggatct gagtgatgtg gtaaagtaca
    12481 ccagactttt ctcaagatac atcagagttt ctaactcatt atttctcata gattcatgaa
    12541 gccgttcctc cgtacgatct gttatcttgt ttatctcctt aagatatgca agaaacaaaa
    12601 gggcattcct ataaaaaatt tgaaatacca gacgactctt tttatgagga ttgagctcgg
    12661 aacgttttat catgatatca agaaattcaa gattaaattt tgatatgagc agtgtatagt
    12721 ctgaaccgat aagtataccc aatgaagctg ttttgtaagg gatctttttg tcatcttcat
    12781 ctctgtaagg aacttttaaa atgagcagtg tactttttcc ggagatatct atacgtgctc
    12841 tttcatcagg atcaagtgaa tcgtatataa ggtcagaatc aatactcagc ttctgagaaa
    12901 tatcatctat ctctgtagat gtaggactta caagatatat taagcagtcc tgagaaaaac
    12961 cttgtgactc aaccagctta ccgtttgctt tttcatagat tcttatcatt tttttcctcc
    13021 tgtattatct tctatggcgg taatgaaaca acatccttgc tactactgct actatccata
    13081 agatcacctc cctattctta atgaattaag tgttatccct ttaagaaatt atgttataat
    13141 ttcaattgta cagttgcaca aacaaagcat tgcattttga aaggggctta gtaatgcaat
    13201 cacaaaatga aaccaatata ttcggccgga tatcaaacat tattatggaa cttagtaaaa
    13261 taggagatat tgaaagttca tactcaactc tttcttccct tataaactct atggtaaaga
    13321 cggaagaaga gtattttttc gcagaaaaag agagtaccct ttcagagatc tttaaccaca
    13381 attacagcag agatatatat gatctaataa aatggggata ctcccagggg gatatttcaa
    13441 tgttccctga gggtgaagga tctattataa tccttcctat aagcaaggga ccaaaagtac
    13501 ttttcgttta tgctgcatac gttaagctac aggaattctc caacgaattc atgatgttcc
    13561 ttagaattat agcatttttg atgggaagtt tatatgaaaa tttgttactt tataacgaaa
    13621 ttcttgacaa gaatgccatc attgaaaaaa ataaaaattt tcttgatact cttatcaatg
    13681 gaagtattga ttctataatt gtttatgata aatccatgaa tatctacttt aaaaattcta
    13741 cttatgattc cataagtgaa aactccttgc ttttggagaa gcttcatgat ataaatattg
    13801 ccaccatcga cagtaacaaa cgtcagatag ttgagcttga gcaggatgga aagttctatt
    13861 ccatagaatc ccttcctata tattttgaac aggaatcatt tgctttatgt tttataagag
    13921 atatctcagg aacaaaggaa cttgaaaaac tcaaacaact cgataaaatg aaaatggagt
    13981 ttctttctat gatgtctcat gaacttagga caccattatc ggctgtaaag gcttactctg
    14041 aaacaatcat caccgctttt gaggatatgg ataaagacac agttctttct tttcttacta
    14101 caattcttac agaagccaat caccttgaaa aactcttaaa tgaccttctt gatttttcga
    14161 agcttgagtt gaaaacgctt aagcttgaac cttctgtctt tgatattgta gaactcatta
    14221 acgacattat aaattcttca gaacagcctg ccactgcacc taaagttacc gtttcttttg
    14281 aaaaatcaaa ggagcacata aaggtaaacc ttgataaaag cagagtgcga caggcaataa
    14341 ccaatatcct tcaaaatgct ataaagtttt gtgacagaga aaaagaatgc tgttttgcca
    14401 aaatttctct tgacgatact ccttcatctg tagaaataaa ggtggaagac aacggtatcg
    14461 gaatcaatga aaagtatttt tccaaagtct ttgaaaagtt ttttaaagtt gattcgtctc
    14521 tcacttatca ggtacagggt acaggaatag gtctttccat ggctaaggaa tttgttgaga
    14581 tgcacggagg aagtatatgt atagaaaaga gtacagaagg aataggcaca acttttaaaa
    14641 ttactcttcc caagggagtg ttatagtggc tgatatatca ataaaagaaa ttaccagaaa
    14701 aattgaggag cttcccactc ccgactttat agtacaaagg atagtatcca tagcttcaag
    14761 cccaaacgcg agtacaaaag aactcaatat ggctatactt gaatcaccat caatggctgc
    14821 taaaatattg aaaatggcaa actctgcata ttatgccatg cccagaagga taacaaagct
    14881 ttcgcaagca attaatattc tcggcttcag gtctgtccgc aacctagcgt taagtgtatt
    14941 taccgcaaaa tcattctttt cacgtgagtt tgaatacttc aacactaaaa aagtttggag
    15001 tcattttatg gccactgcaa tttcctgtga acttctttcg aagtttataa actttcctga
    15061 ccgagaagaa gcttttttgt gtggtatgct tcacgatctg ggcaagatgg caatgtctat
    15121 tgtaatgccg gatgtttttg aaatgatagt taccgtagcc aagcataaaa aaatttcctt
    15181 cagtaaggca gaagagctac tggaaaccta ttcacatcag cagataggaa gaatgctttt
    15241 tgaaaactgg cagatgtctg aaataatgat agcttcagct acttttcatg attctccgtc
    15301 actatgcaag tctgaagact caagaactat agtaaatctg gtcagcatcg caaacactac
    15361 ggtaaacata ttattatatg gttattcagg atgttttgat attccagctg tttcagatga
    15421 agcatggaac aatcttggcc tgacacccag aaaatatctg aactactttg aaaatttaaa
    15481 aataaaaata aaagaatcag aagacttcat gaacttatca aatgttcttg atgatctaca
    15541 ggaaggtgag ctagatggca cagtttaaaa agcagatgtt ggaatctgaa atagaaaaaa
    15601 taataacagt atctttgaga gatataaaag acccgagagt ttgcgggaag atactgacta
    15661 taaacagagt tgatatgaaa aaagataagt cccttgtcga tatctatatt tcatgtcttg
    15721 acaactctga cctgaaggaa actgttgatg ttctgaacag agccaaaggg tttttccgca
    15781 gtatgattgc tcagaacata aatattttca aggtgccaga tgttgcattt catgaaaata
    15841 ggggtataga gcagagcgtt aaaattcaca aacttttgga tgatataaag cagatggatg
    15901 agaagaaagg taaaaaatta tgaacgatgg aattgttata gtagataaaa aggaaggaat
    15961 aacttcacac gaagtagtaa gttattttag aaagaaattt gatactccta aagtaggaca
    16021 ttcggggaca cttgatcctt ttgcaaccgg tgttatggta gttggcataa atagaggaac
    16081 caaaattctt cattttctat ctgcacaacg taaaaattac catgtaaccg cagaactagg
    16141 aaaaataacc gatacatttg atatttcagg aaaggtaaaa gaaataaatg aggttactaa
    16201 tgaaaacctg ctcaatttag aaagcgttgt tatgtctttt ataggcaaat ataagcaaac
    16261 tacacctgct tattcagcaa gaagatatca gggaaaaaga ctttatgagt atgcaagaca
    16321 gggaaaaata atttcactcc ccataaaaga agtagaaatc ttttctatag aagagttgat
    16381 gcaaaatgga aaagagtttt cctttacggt atctgtttcc agcggaacat atatacgctc
    16441 tctgattatg gatatcggtt acaaactcgg ttgcggagct gtcaccacaa agcttagaag
    16501 acttaagagc ggatcgtttg atatttcaaa atcaaaaaaa atggaagata tttctttttc
    16561 cgatattata agcattgaaa attcacttga ttttccaaaa atacatgttt ttgacggaac
    16621 taaagtcata aatggagtac agatctttaa aaacgacata atgagttacg atgaatttac
    16681 taaaggctct tatattaggc tatttgatga aaataatgag tttcttgggc tgggagtagc
    16741 tgaaaaaaat agttctttta tgccttttct cttaaacagc actgacagaa acgacagagt
    16801 agtcaagatc ttcagaatca tgaggtgaaa aatgtacgca cttgctatag gaacattcga
    16861 cggaattcat aaaggccacc agtatattct gcaccagaca caaaaagttg cacaggagaa
    16921 taatctgaaa ccacttgcat atatgatacg ttatcccata gataagtatc gcgggaactt
    16981 ttctggatta atattgcctt catggagaag gaaggaactt gttgaagata tggggtttga
    17041 agtggagatt tttgacatgc aggatctatg ggatattacc catatagact atatagacat
    17101 actcctaaac aagggagtga aacatattgt ttgcggaagg gacttcacct ttggaaaagg
    17161 aaaactggga aatgttgatt atatattgaa tcaagcgacc ccaaagggtt tagggctcac
    17221 cgtcctgcag gatcttaaag agtacggaag cagagtcagt tcaactctta taagaaaggc
    17281 tttaaccgat ggagatattg acttagccaa tgaaatgctt acacgaagct ggtcggttga
    17341 aggaagagtt tatgaagaca ggcacgtggg ttttaaattg ggatttccaa ccgccaatat
    17401 tgacttcaga gagaaggagt ttgtgatatc cccaaagtac ggagtgtatc ttgtgaaaag
    17461 cagtcttccc ggttattcaa gaacattttg ggggcttatg aatgtaggct tgcgcccgac
    17521 ttacagagaa aatctaaacg gtcccaaggt cgaggtgtat ttcatggact ttttcggaga
    17581 tctttacgga aaacacctca aagtggaagt tataaaatat ctcagggaag agatgatgtt
    17641 tggttcagaa aaggacttga tatcacagat gattaaagat gaagatactg ccagaaaatt
    17701 agttataacc atggaaaaag gggcttaaag ccccttttta tttttcaacg gaaagattaa
    17761 gttcaatata acagtctttt gaaacatcaa aatcaagcca tactattcca ccctgggact
    17821 tattgtcaat ttttgaagct actcctttat ttgtaaagga gtcaagagtt attccgggtt
    17881 gactaaggta tatccttgtt ttataagttt ctgaagaagc attctgaaac tctattttta
    17941 cctgcattat tctcttgtct tttccgccgg tcgtattgta cgttacatct ttggtaatgg
    18001 ttgatttata ctttagactc caggaacttt ttaaatttat ccaagcctct gaatcctttg
    18061 gagtatctct tatatatcct ttctttatgg caaaagtatt ttcatcaaaa ttctccatca
    18121 gccatacttc tcctgaagca agatcttttc ctatgccatt tgaaacggta ttgtcaaaat
    18181 atctgattat acttgccggc tgaggatctg tgatatttga atataggttg ggattgacgg
    18241 tatatctgtc ttcaaaagaa gcaagcttgt atctatcggc agttaaagcc atatactcac
    18301 tgttcatgtc aaccatccct agatcaaaaa aaagtgtatc tgactgcacc tgtgctccgt
    18361 atgagtttga agaatcagcg gcatatgcaa tgcttttggt tctcaaaaga gcctcctgct
    18421 gatagtatcc tgaagatata ttcacagaag agtttacaag ataaagtctt gtttctccaa
    18481 cgggaacacc gttaatctct agtttactta tgaaatttcc catattatca atctcgtatg
    18541 tatttttcca gttcccgcta gcataaagac ctatgaatgt cttaccctga gcatccagaa
    18601 tcaatgtcct tttggatctt atactgtcaa gattcttttt atctgtaaga caccatttag
    18661 atatcctaga attataaaac aagatgtcct gccttccttc aatttcaaaa acattggaat
    18721 atatttcctt aagtgaaaat tgtgaaaaat cgtttatatc tactagctta aaagggaaag
    18781 ccgtgttttc ttcatccaca taccagctct tcgcaccact cacccataaa aggttaaagt
    18841 tatctggtat gctgaatcta ctttcgtcat actgtaaata cgttaccagc gcatccctaa
    18901 aaacaaaagt atatctctgc tgtcctgcca gcataacaac acctgtcata ataacaaata
    18961 gcgcaataaa aagttttttc atcttttccc ctccttatcc taaagtattt tcttataata
    19021 ttattccttt ttacacaaaa tttacatcca ctgcagtgac agtacaactt taaaaagtac
    19081 aatatctgtc agcaaagcaa attaatttat gggggtaata tatgaacttt cttgtttttt
    19141 ttgtgtcaat tgctcatttt accgttgatt tttttgcaga tatttttcaa cccctgattc
    19201 cgttttttat gcaaaagtat gcaataaccg accagccgaa aacacttgtt cttatggctt
    19261 cagtattaag tatgaccgct tctatgagtc agcttataat aggatactat actgacagat
    19321 cttcaaaaaa gcttattctt atttatttct ttgttgtttt ctcaatcttt cctggatttg
    19381 ttatgggtta tgcccccaat ctcacctatc tctttttcat atttctaata ggctatattg
    19441 ccaacgctgc gcaacatcct cttggcgcaa gcattgccgg ccatgttgga aaagggaaaa
    19501 gtctgacatt agctttctat gttcttggcg gaactttcgg ttcaggctta ggacccattg
    19561 ttattacgtg gtatgtaaag aattttggaa tagaaaacac atgggcaata ggtttgatca
    19621 tgtttgtcat gttttctatt ttaactcttt atgcttataa atatcttaaa aacaatgaaa
    19681 tattaaaacc ttcttccaat acagcctccc tgctccaagt aataaagagt cttaaagttc
    19741 tttatccaat ctggatactt acagtatgca gatctttcat atcttcttta tttcatatgt
    19801 atggaccaat acgtaccaac tctatgggct atgacctgac cgttggtgga cttatgctat
    19861 cttcagcact tatatccggt atgttcagca atatgcttgg tgcaaggatc tatgaaaggc
    19921 atggaatcaa gaaacttaac gttctatcct tcagcggata cactgttttt cttctaatgt
    19981 ttgcgctaag caaaaatatg attttcatag ctttatcctt tgtacttgct gatatattca
    20041 tgttcttcac tatgtctgcc aatgtgagtt atgcacagaa gattcttccg cagttcagag
    20101 gtgtggcttc gggagctctt atgggttttg catgggcttg cggaaatttg atagtcatgg
    20161 ctttttcctc agtatttggt aacaatatag agtttatgat caactccttg tggttctttg
    20221 ccgctattgg cattatcctt acctttatac ttttcaggaa aaaaactgta ttaacataat
    20281 tatggtaaaa tcttgataat aactacttaa accggggtga ccaatgagaa cagaacttta
    20341 cacatggaag tacatggaac ttggtgaaaa agttgtttcc acactaaaag aaaaaagaca
    20401 cgatgcttat cttgtagaaa aaaaagagga cgtaattcaa aaagttaaag accttattcc
    20461 ctcaggctca aaagttgctg taggcggaag cataacttta aatgaatgcg gaattcttga
    20521 acttctacga aacagggact atgagttcta tgacaggtac tctgcaaaaa atcacgaaga
    20581 atctatcaga ataaacagac aggcatttga aacagatttt tttctctgca gtgcaaatgc
    20641 tataactctg gatggaaaaa ttgttcagct ggatggaaca ggtacaaggg tggcacctat
    20701 gatatggggt ccaagaaatg taataatagt tgccggcatg aacaaaatag tatcttccat
    20761 tgatacagct ctggaaagaa tacgctttat atcccctatg aatgccaaaa ggcttaatat
    20821 aaaaactcca tgcaccagaa taggagaatg cataaactgt gagtcatcag aaagaatctg
    20881 cgttcatcag gtgatagtag aaaccggagt aagaacaccc ggaagattta agataatttt
    20941 ggtggcacag gacctggggc tttaactccc ccggtcctcc cttttttatc cagagtatat
    21001 acaaaagcca gaagttccgc cgccacttta tacatctcgg gcggtatttc tacattaacg
    21061 tcaactgcat aaaacttgtc tgcggcagct ttatcctcta taatcgctat cttctcttta
    21121 cgcgctactt caattattct ttcagctatt tctcctattc cctttgcaat tacctttggg
    21181 gcggtatcct cacctttttt gtatctaagt gcaaccgctt tttttttatc atctctcata
    21241 gcttctcatc cttttaatca atttcaaaaa cagttatttc aggaggacaa aagagtcttg
    21301 cattaaacca gctccctatt ccgctgttta cataaatatc gtaattctgt tcattgtaca
    21361 tgccttttat gaaactctct tttgaaaaat gggactgcag cagaagcctt gttaagaaag
    21421 gaagatttat ctgcccaccg tgagtatgcc cagccagtat aagatctgca ttatctataa
    21481 aagaagagaa cttatatata ttagcaggaa catgtgtaag aaatatgtta tactctcctt
    21541 tgtcaaaata atactgtccg gggctttcat acataggcag gctggtaaaa gctattgttt
    21601 tattcttttt gttcaatcga acctgtt
//
LOCUS       DAOSHR010000009        20468 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_2119_length_20468_cov_5.959144, whole genome shotgun sequence.
ACCESSION   DAOSHR010000009 DAOSHR010000000
VERSION     DAOSHR010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 20468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20468
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2119_length_20468_cov_5.959144"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..972)
                     /gene="nrdD"
                     /locus_tag="PLU28_00970"
     CDS             complement(<1..972)
                     /gene="nrdD"
                     /locus_tag="PLU28_00970"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HOG33666.1"
                     /translation="MKNTILSYIDMSDWKVKENSNMQYSLQGLNNHIHQQAIKDFWLE
                     DIYGQKDSKIPFYHNSGFFHIHDLGCLSSYCVGWDLYDLLQRGFGGVENKISCGPAKH
                     FRTALGQIVNFLYTMQGEVAGAVAFSNFDTLLAPFVYYDGLSYETVKQNMQEFVFNMN
                     VPTRVGFQSPFSNITFDLSVPLNFSDMKAIVGGVEKDLTYKHFQKEMDMINTAFVEVM
                     NNGDYEQRPFSFPIPTYNITKEFNWKSPVAEKILELTAKYGTPYFANYISSDISPQDV
                     RSMCCRLRLDNAVVMDHIGKLSQSFNISGSSYEHDTNIRRGGLFSSNP"
     gene            complement(1139..2482)
                     /locus_tag="PLU28_00975"
     CDS             complement(1139..2482)
                     /locus_tag="PLU28_00975"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HOG33667.1"
                     /translation="MRTLYITLSFIFLLSIFVFFNIPSKIRAEKGVVRLDTNFSESHS
                     YSLLTGEWEVYSDRLITPQEFSAGGFSSTMFRNFSKPSFFSSDLDGAMVTTLRMTVHT
                     KDSNSPIALYFPNMGCGFTVWADGKALNNSANGCMKSCGQSCIYQNVNFIPSSDSFDI
                     VVHMENTGFSQKSALKNIRLGSPDSINRYRMSFLAGEMFMIGCFLITGLYGLFLFFKN
                     KSEKKMLILSLLCFIEPLRNLLSDSSFFHYILGILPWNLQTRLSIPVMTVIAFMVFLF
                     VSELFDFSKGRAAIKVYGAYGAGVSMAGFFLAPAYWSTVYFFYLLFIFFISAYVCFIT
                     GYALLKKVRIPFSFVVAGIGFLYFQLLGSLISGQYLGYDDFTCLGIFVFAFTQILWLS
                     LKSESSYINEVSLESSIINEELKSLNAMLELQLSQKSFEIQQLQKKIHQHHKKLS"
     gene            2699..3064
                     /locus_tag="PLU28_00980"
     CDS             2699..3064
                     /locus_tag="PLU28_00980"
                     /inference="COORDINATES: protein motif:HMM:NF028342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOG33668.1"
                     /translation="MFFIGIFGVDSSEKILKQLSSLVCPGCGRLCSGKVICESTFFHI
                     FFIPVVRWNKKYFLRTDCCGCEYMLTKEKTENILKGDPLDFQDTEYGKCRSSFCPECK
                     YPLNSDFSYCPRCGKKIGV"
     gene            3065..3337
                     /locus_tag="PLU28_00985"
     CDS             3065..3337
                     /locus_tag="PLU28_00985"
                     /inference="COORDINATES: protein motif:HMM:NF015889.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015661 - L-lysine efflux transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903401 - L-lysine transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysO family transporter"
                     /protein_id="HOG33669.1"
                     /translation="MLWLILAAFVAGVVLGINQKLLFIKKLKPVSIITVLLLFFMGYE
                     IGSDAQLVSRLPQIGIKALLTAVFAIAGSVLFTTLYDKMIKKGDRK"
     gene            3334..3909
                     /locus_tag="PLU28_00990"
     CDS             3334..3909
                     /locus_tag="PLU28_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine exporter LysO family protein"
                     /protein_id="HOG33670.1"
                     /translation="MILLISSVVLGILCGLFFNFPINSNIITILLMILVFCVGLDIGS
                     QQEIGKKIRSKIKTILIQTALTICGSLLFAGISGFFTGLSFKEAAGAGAGMGWYSLSG
                     VMISDLYSAELGAVSFTANVIREVLAIVTIPLFSKVSELGAISVGGATTMDTMLGVVS
                     KSTSAENTLVAFGQGVLLSMAVPVLITLIFS"
     gene            4112..5731
                     /locus_tag="PLU28_00995"
     CDS             4112..5731
                     /locus_tag="PLU28_00995"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF015536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HOG33671.1"
                     /translation="MLKKSIALLLVFVSIISLSFACTIIAVGKDAMADGSTIITHSCD
                     SGSDNPLLWIIPEADWAEGSVREIYQNAHYFYFKGNKNGTPVYNKDGYLTVDETVAKK
                     VGEIPQVSHTYRYFYCGYSFMNEKGVAIGESTFSQGNYATDTGKQIMKDLLEDTWGII
                     DCFAAQDIALERASTAREAVQIMGDLVNKYGWYGAGEIMDITDGNEVWVAEFYGRDLW
                     AAVRVPDDSVFVGANVGILRDLDLTDTKNVMYSPNLVKFAVEKGWYTPKEGEKLFSPG
                     PVYAPRTGMNVREWRATDLLAPSLKVEYNQTYYPLFVKPDKKLTVQDIFTIHGDVYEG
                     TDLDLTKGIGAGPFGDPFASYRIGGRARPIGVPQSTYIHIGQVKGWLPDEIKGISWFG
                     YGAGATTYLTPLWPIMEKLPDFYMVGSRYEPLRRDSAFWTNLYVQEVVRHRYMDAIKD
                     LRAYRDPKMQSIYTQADNVQNQAAAFIRSGNKEQGIKLVSDFAYDTAIAWQRNWLDLG
                     DTIFAKYVMDRIYMKGPSYPKTWQTTITPVK"
     gene            complement(5758..6429)
                     /locus_tag="PLU28_01000"
     CDS             complement(5758..6429)
                     /locus_tag="PLU28_01000"
                     /inference="COORDINATES: protein motif:HMM:NF020121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase trimerization
                     domain-containing protein"
                     /protein_id="HOG33672.1"
                     /translation="MHKINGVCKDFDYVKALQDIYSAFRYFGYFVDFIFRDSKIENYE
                     IVVVPYALYIPKNFMKKLEKYQGSLFITCMSNIKDENNSIISDRPLGWKIKDLSFTIK
                     DFGAIYEEYIDYKDHGLVADMWIEELEVIEGKQTGTWESSPLKKGPALIAAKNSDITY
                     ISTVLSKKSWRKLLKYTFKPELEIKESLELITNEKESYFINFDSEEQRSGHTDIEPYN
                     YKKTK"
     gene            complement(6459..7115)
                     /locus_tag="PLU28_01005"
     CDS             complement(6459..7115)
                     /locus_tag="PLU28_01005"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOG33673.1"
                     /translation="MSTEISARLTKHGEPVFWSQIYNSFSQITTPKKIPAIPNPHQML
                     DFYRFSSISARKFAQMQIDIIRKHSDKPVTTNFMVNFTDMDYCSHQELYDFISFDNYN
                     PSPKFDPLQQAFNLDLMWSLKRKNFTVMEQQPGRVNWQNRNLFFPAKWLVPSTIQAFL
                     HGADNLSYFRDRAVSFGAEQYHNGLINYEGNFDTSPRLKVVKNFLKKLISFLQDPRPR
                     "
     gene            complement(7000..7608)
                     /locus_tag="PLU28_01010"
     CDS             complement(7000..7608)
                     /locus_tag="PLU28_01010"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOG33674.1"
                     /translation="MLGTAYYPEHWEEDRIEKDLSAFRNNGIEFVRIGEFMWSVLEPE
                     EGKYNLSLLKKTMDICKALQIKVILSTPSATPPAWLISKYPQILQKDIVSDTKNFGGR
                     RHYCYNSKEYALYVCKIVELLACEFSSHDALYAWQIDNEFGCEDTSHCFCEKCDENFR
                     TYLEDKYGDIGSLNKAWGTGLLVTDIQQFFSDHHPKKDSGYS"
     gene            7886..9247
                     /locus_tag="PLU28_01015"
     CDS             7886..9247
                     /locus_tag="PLU28_01015"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF013071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HOG33675.1"
                     /translation="MVEKERVSLCNDKEVKNGKYVLYWMQASQRAHFNPALEYAVLVA
                     NTMNKPLIVLFCIMPEYLTASFRHYQFMIEGLQETAQTIRGYGAAFIIKAGDPCEIIK
                     ELSESSSMIVFDFAYLDFMKAVRKKISAQTACRVVQVEGNVVVPVAAASSKEEYSAAT
                     LRPKVSAVISKYLNSEEEITELKNKISDPQIESLTEQQAKEYIKKFRTNNRYFDESRF
                     IGGYSHARQKLDDFIKYKLHNYADMKNDPTSEVQSDLSPYLHFGQISPVYIANQITKV
                     LSESAQEYLEELIVRRELAHNFVMYNDDYAQYNSVPAWAKKTLAEHLSDPREYYYSPQ
                     QLENAQTHDEYWNSAQNELLLTGKMHNYMRMYWGKKIIEWSETPQKAFETMVYLNDRY
                     EMDGRDPSGYAGICWCFGKHDRPWSPRSIFGNVRYMNDKGLERKFKIQKYVQRIKDLK
                     KGS"
     gene            complement(9249..9749)
                     /locus_tag="PLU28_01020"
     CDS             complement(9249..9749)
                     /locus_tag="PLU28_01020"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HOG33676.1"
                     /translation="MSDRRYMRELSLINRLETEKALIDTVNGVNTEVLDKIQKDFIKT
                     FFSETRESSAVIRETLRIISSDSQADQESVIALFRSFHTIKGLAGFVSAEMVRIISAK
                     TELILDACVKGRLLMDDNMINIVFYSSRIIDNLCDRYQNGVAQEYLEYIKEHMGIIED
                     KIKEID"
     gene            complement(9754..>10089)
                     /locus_tag="PLU28_01025"
     CDS             complement(9754..>10089)
                     /locus_tag="PLU28_01025"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG33677.1"
                     /translation="YAVCDIANDGAQGISVFESSLKTNKRYDLVCLDIMMPQMDGIQV
                     LQKIRSAENKNGVYGLDGTKIIMTTALSDVENVRNSFKAQCEAYIIKPILKKDFILKL
                     QELGLLKGG"
     assembly_gap    10090..10189
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10190..11385)
                     /gene="aspS"
                     /locus_tag="PLU28_01030"
     CDS             complement(<10190..11385)
                     /gene="aspS"
                     /locus_tag="PLU28_01030"
                     /EC_number="6.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF003483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA(Asn) ligase"
                     /protein_id="HOG33678.1"
                     /translation="MRQRIKELQGLKSEAQVCICGFIKTVRNKGKNLKFMIIDDGSSQ
                     IQATCLRNLLGEKIDALDKISVNSVLKISGTLNRTDLSKSGTEVLVTDLEVLSSAHTG
                     LPIDISEELESSLEKRLNWRHLDLRNPKKKLIFRIFSDFLKLSREYFYARHLTEISSS
                     KLMAAASESGAQVFQVNYFERKAFLAQSPQFYKQMAIAGGFEGVFEIGPVFRAEPSFT
                     SRHTTEFTSLDVEIAYVETVEEVMCFEEQWLAFTFSGLKELYSKQLEELYGIQINIPE
                     LPFPRISMTQAQGIVKSYGIECLENSDLNSDGEKALGRYVKDVFNSDFVFLTDFPWSV
                     RPFYHMRSENKQITKSFDLIYCGTEITTGAQREHRYDILCTQATEKLLDLKGIEEYLN
                     FFRFGCP"
     gene            complement(11575..12018)
                     /gene="pgsC"
                     /locus_tag="PLU28_01035"
     CDS             complement(11575..12018)
                     /gene="pgsC"
                     /locus_tag="PLU28_01035"
                     /inference="COORDINATES: protein motif:HMM:TIGR04011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0070501 - poly-gamma-glutamate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein PgsC"
                     /protein_id="HOG33679.1"
                     /translation="MNPETFSVGISISTLYWWLTGLSAGGIVTPFYFYMFLNNPLRIL
                     YTLGSGLITYLLVLLMNRFLILYGKKRLAVNILTGILVKLLIDYITIRNLPVDYISTT
                     IGTIIPGLIANDFYRQGVLKTIFSIGIVTAIIYFLILFLKVVLSF"
     gene            complement(12015..>12261)
                     /locus_tag="PLU28_01040"
     CDS             complement(12015..>12261)
                     /locus_tag="PLU28_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG33680.1"
                     /translation="FNPRDDRQDRTLCFESFLADGRFKKLLSTHRLPSKMGKNIEYTV
                     IKDVRKISELIPQNSLVFGFGNIRDISAWLMTLEVER"
     assembly_gap    12262..12271
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12272..13120)
                     /locus_tag="PLU28_01045"
     CDS             complement(<12272..13120)
                     /locus_tag="PLU28_01045"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HOG33681.1"
                     /translation="MNYILTAFLIVIVVIVYENIRLYLCKKTIPIRIQVNGTRGKSET
                     VRLVHAALTAMNYRVLGKTTGTVPYWILPGGSFKKINRIAPANIQEQYKLIYKAKKMK
                     CNAVVAECMAVNPEFQSACARIVQPDITILTNAYTDHVQEIGENEVQTLKTLLLSIPQ
                     GSRVVVGDISPEGKLFLDVQAVRKDLQLYEKDDSFPVQKNDFKFNVFEDNIRTALKTV
                     ELLGGDLDASYEGMKEVSPEVGSFKFLKISNSVILANAFAANDLLSTEKLFEYSKKIY
                     PDKTMIG"
     gene            complement(13117..14301)
                     /gene="pgsW"
                     /locus_tag="PLU28_01050"
     CDS             complement(13117..14301)
                     /gene="pgsW"
                     /locus_tag="PLU28_01050"
                     /inference="COORDINATES: protein motif:HMM:TIGR04332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate system protein"
                     /protein_id="HOG33682.1"
                     /translation="MSQNLIKYANNQRYNFYKLYRQVTPINSFTKSTIPFFYLFLAFV
                     ISVFGVCFIEKTKVSTKNDYYDLQYKASHIMQNSISVLKDYRIENNISINIDDDPNRT
                     GIIGEDMTLLVTSVGNLQAKRTSTNPDFAALMVKFFKELDLKAGDHIAIGASGSFPAL
                     LIASLSAAQAMELKPLIIYSIGASNYGATIPGFTFIDMLRELNRQNIFDYKLLAVSLG
                     GDHDLAQYMFFEESEAVFDQIAQSAAVTIIKEATLEKSIKKRMELYAGALGSNPMKVF
                     INIGGASTNFGATEASLIFPNGLSKSPPRVLSSPVKGLIFEYSQSGIPVIHLLNIKDL
                     AMKNKIPVDPIPIPQLGTSGVFFHFKYRMDYLAIFSGLTIFILITGYREKKAKKRSGE
                     KL"
     gene            complement(14356..16014)
                     /locus_tag="PLU28_01055"
     CDS             complement(14356..16014)
                     /locus_tag="PLU28_01055"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF015536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HOG33683.1"
                     /translation="MRKSWLVLMMLVVVMLATTISYACTIVGAGNKAMADGSTVITHN
                     DDSTSADFRLWIIPEADWSEGSMRDIVLDSHDYIDFSNFPKSVDYTKNRNGNAMVVGQ
                     MPQVAHTYRYFHSRYSFMNEKGVAISESTGGIDTSTEYGKKVAKYFSDNEGIIDCWYA
                     QDIALERSASAREAVKVMGDLVETYGWSNVGGGPESMFIGDGKDVWLAEYYGADLWVA
                     VRIPDNAFSVTANRLRIMEVDPNDTANVMYSKNLFKTAVEQGWVSKDLPLNQWKLAEI
                     YSPNDSLYATRREWRALDLVAPNYMKANNYGPSELRYPLWVVPEKALTIDDIWKVKAD
                     YYEGTPYDLTKGAAAGPWGDPLRYANSSKTEKTATWERSINMHRTCYLHIGQTKAWLP
                     DPIKGVSWYGYGAPDTTYLTPLWAAMTKLPDFYTIGSRYEGFRRDSGWWVNTYVQQMA
                     ELHYNEAIKDIRNLRAPKMDMLYKVTPQVQELAGQLYRQGKTKEAVALIDNYGYMNAV
                     AWNTEWLKLGDYLLGRYAMGYINFRTTPYPQTWNDTIGFTTPQR"
     gene            complement(16096..17757)
                     /locus_tag="PLU28_01060"
     CDS             complement(16096..17757)
                     /locus_tag="PLU28_01060"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF015536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HOG33684.1"
                     /translation="MGKKRILVALFTLIVLLVSTVAFTCTIIAVGNKATVDGSAIITH
                     NDDSSVADFRLWILPGGEWQSGTVRDIVLDSHDYIDYSNFPNVDYNKNRNGRAMVMGI
                     DPQPAQTYKYFHSRYSFMNEWGVAIGESTAGITTSNNYGKAVRQALFEDEEGIVDCWA
                     AQDIALERATTAREAVQVMGELINKYGWSVAGGGGELMDITDGKEVWIMEFYGANLWM
                     AVKMPDDMVYVGANTMRILVDPRDGIDPVSGVKYEVLTSPGFVEYAINKGWYDPNSGK
                     KFRPAEVYANRNAHNKREWRVFDLVAPSMKIEDNAKEYPLFVKPDKLLSVDDIFKISG
                     DHFEGTQYDLTKGPAAGPFGDPLTNYGLASRQRPIGIPLTAYVHIGQVKAWLPREFRG
                     ISWYGYGSVGHTYITPLFPAMEKLPDFYAVGSRYETFRRDSGWWTNTYVQQMVGLRYS
                     EAIKDLRALRDPKMQSVYTQTSVLQEAAATMYRNGDTKGAVKLMSDFCYNTAVTWQRD
                     WLELGDRLFTKYAAERIGFSSSGYPQWWKDWLTPSKNPEFSKYGY"
     gene            complement(17812..19158)
                     /locus_tag="PLU28_01065"
     CDS             complement(17812..19158)
                     /locus_tag="PLU28_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG33685.1"
                     /translation="MNKENKSAPVWFKLIVLVLIGVMGIIAGMDFWNLRHYKESLVAS
                     EGLTKQIMLSDYLPSLKGTWGDTPIYVYDSGVPGGVALLMGGTHPYEPATMMAGYILA
                     ENMKVEKGKVFVVPVANRSANTLGMLGNAYPMFYTIDTAFGPKQYKIGDRNTNPLDQW
                     PDPFTYVHYPSGQNLTYQDMRNFNRTWPGRADGTLTERISYAFMELMRKEKVDIYFDY
                     HEASLMYPVVDTYVAHNFADDISMLAYMTLDTYKFPMKAEASPKKLRGLSHREVGDYA
                     IDEELVPELVKLEMSGYAYDYDKKLEVINKSSHHTFVLLMETLEPFIERVAGRVTVDL
                     LMEGNDEFIQTAADKKLLFARYDSREGITMPIDEDGRGGYVVKGAPMYYRVARHLEGT
                     KEAIKQMSMLYPDKELVLDFPGFHDIMEPATTKGNAALGDFLHDPATSDPSRVFYQ"
     gene            complement(19173..19346)
                     /locus_tag="PLU28_01070"
     CDS             complement(19173..19346)
                     /locus_tag="PLU28_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG33686.1"
                     /translation="MLIIQEIIHQLYFVLAAIICLFLVKNLFKRNKRQGWVYDIVYAY
                     VIVTFILRVLHIK"
     gene            complement(19365..>20468)
                     /locus_tag="PLU28_01075"
     CDS             complement(19365..>20468)
                     /locus_tag="PLU28_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG33687.1"
                     /translation="VTASLFINIYSETGAMDALVRKLVNRFYNHKWIMMIFMAIFMII
                     PGALTGAGSVSIFVVGGLVTTVLTYMGLSKKQIVGFMYMFAMLAAAAPPINLWAMLMT
                     AQANMPYVGFDVVLLAPIAVISIFTIIWYGRGAKPKGKEEILASLPPEVPGMNWFRIL
                     LPLVALIILILLQKYAAFSTPIFGLPLTFVLCTVVALLCNPKKTTFKKWLEVMSNTME
                     QVFPLLATVISVGALVNVMNATGVKGLIAITFVTLPVYLIYALVLVFGPFAQGSLSYG
                     SAVILGTPIIFLFNNLGFNVTVVTAALSLIFPIGDCLPPSRIVGRLSIESVKYEEGYM
                     SFLKSALVPILFMAAVALIMFIFPNQFKFLVIS"
BASE COUNT         6044 a         4018 c         4083 g         6213 t
ORIGIN      
        1 agggtttgaa ctgaaaagac cgccccttcg tatgttggta tcatgttcat aactgctgcc
       61 tgaaatattg aaagactgtg aaagcttacc gatatggtcc ataacaactg cattgtcaag
      121 ccttagtctg caacacatgc tgcgtacatc ctggggtgag atatccgaac ttatgtaatt
      181 tgcaaagtaa ggagtgccat actttgcggt gagttcaagt attttttcag ccacaggact
      241 tttccagttg aactctttgg ttatattgta agtaggaatc ggaaaagaaa acggtcgctg
      301 ttcgtagtct ccgttattca ttacttccac aaaggcggtg tttatcatat ccatttcttt
      361 ctgaaaatgc ttgtaagtaa gatctttttc cacacctccg acaatggctt tcatatccga
      421 aaagttgaga ggcactgaaa gatcgaaggt gatattgctg aaaggtgatt gaaatccaac
      481 tcttgtaggc acattcatat taaaaacaaa ctcctgcata ttttgtttta cggtttcata
      541 tgaaagtccg tcgtagtaaa caaaaggagc cagaagagtg tcaaagtttg aaaaagcaac
      601 ggctccggca acttctccct gcatggtata aagaaagttt actatctgtc ccaaagctgt
      661 tctaaaatgt tttgccggac cgcaggagat cttattctca actcctccga agcctctttg
      721 aagcagatca tacagatccc agcctacaca gtagcttgaa agacagccaa gatcatggat
      781 atgaaagaat ccgctgttat gataaaaagg tatcttactg tctttttgtc cgtatatatc
      841 ttcaagccaa aaatccttta tggcttgctg atgtatatgg ttgttcagtc cctgaagaga
      901 atactgcata ttggaatttt cttttacttt ccagtctgac atatcgatat aagaaagaat
      961 agtgtttttc ataaagcacc cccataaaaa atatttccaa acggttttcc cgtttagaaa
     1021 tattctacta caaaatacta tatattgtgt gttaattttt ttggatacaa tatgtcgtta
     1081 ccttagacac accacagaca cacatttgct ctgaaaagta atttctgtta attcaaaatt
     1141 aagaaagctt cttatggtgc tgatgtatct ttttctgaag ctgctggatc tcaaagcttt
     1201 tctgagagag ctgcagctca agcatagcat taagagattt aagctcttcg tttattatgc
     1261 tgctttcaag ggaaacttca tttatataag aagactcgga ttttaatgaa agccaaagta
     1321 tctgagtaaa cgcaaaaaca aatattccaa ggcacgtaaa atcatcatat cccaagtact
     1381 gacctgatat taaagagcca agaagctgaa agtaaagaaa tccaataccg gcaacaacaa
     1441 atgaaaaagg aatccgaact ttttttaata aggcatatcc ggttataaaa caaacgtaag
     1501 cagatataaa aaatataaaa agcagataaa aaaagtatac agtactccaa taagcaggag
     1561 caaggaaaaa ccctgccata gaaactccgg caccgtatgc accgtaaact ttgatggcag
     1621 ctctgccctt tgaaaaatca aaaagctccg aaacaaaaag aaaaaccatg aaagcaataa
     1681 ctgtcataac cggtatggaa agtcttgtct gaagattcca cggcagtatg cccagtatat
     1741 aatgaaaaaa actgctgtct gaaagcaaat tgcgtaatgg ttcaataaag caaagcagtg
     1801 aaaggataag catttttttt tcgcttttat ttttgaaaaa caaaaacagc ccataaagcc
     1861 cagttataag gaaacatcct atcataaaca tctctcctgc gagaaagctc atgcggtatc
     1921 tgtttataga gtccggagaa cccagcctga tattttttag tgcagatttc tgagagaaac
     1981 cggtattttc catatgaaca acaatatcaa aggaatctga tgacgggata aaattaacgt
     2041 tttgatatat acagctctgt ccgcaacttt tcatgcatcc atttgcagaa ttgttcagag
     2101 cctttccgtc tgcccagacc gtaaagccac agcccatatt tggaaagtac agcgcaatgg
     2161 gagagttaga atcttttgta tgcacagtca ttcttaaagt tgtaaccata gctccgtcca
     2221 aatcagatga aaaaaatgac ggtttactga aatttctgaa catagtcgaa gaaaaaccgc
     2281 cagcggaaaa ctcctgtgga gttataagac gatccgaata tacttcccat tctcctgtga
     2341 gaagactgta agaatgtgat tcagagaaat tggtatcaag cctgaccaca cctttttcag
     2401 cccggatttt ggaaggaata ttaaagaaaa caaaaatgct gagtaaaaaa ataaaagaaa
     2461 gagttatata caaagttctc attagtttag ttaccccccg tataatatct tcacatatac
     2521 catatcatac aaagattact aacgaatata tatctatgtt taattttgtg taaaatgaac
     2581 tgaattcttt ttagtgacaa agattggaga tttcacagaa cataggataa acataacggt
     2641 aaatactcta tttgtgctac aattaatgta atgaagcgta atttcagaga ggtggagtat
     2701 gttttttata ggaatattcg gggtggacag ttcagaaaag attttaaaac agctgagctc
     2761 tcttgtatgt ccagggtgtg gcagactttg cagtggcaaa gtaatatgtg agagcacatt
     2821 ttttcatata ttttttattc ctgttgtcag atggaacaaa aaatattttt taagaaccga
     2881 ttgttgtggc tgtgagtata tgctgaccaa agaaaaaaca gaaaacatac ttaaaggaga
     2941 tcctttggat tttcaagata ctgagtatgg taaatgcagg tcttctttct gtccggagtg
     3001 caagtaccct ttaaattcgg atttttcata ctgtccgcga tgcggcaaaa agataggtgt
     3061 gtagatgctc tggcttattt tggctgcttt tgtggcgggt gtcgttttag gaataaatca
     3121 aaaacttctt ttcattaaaa aacttaaacc tgtaagtatc ataacggtac tgcttctatt
     3181 tttcatggga tatgaaattg gttcagatgc acaactggtt tcaagacttc cgcagatagg
     3241 tattaaagca cttcttacag cggtttttgc tattgcggga agcgttcttt ttacaacact
     3301 gtacgataaa atgattaaaa agggtgatag aaagtgattc tgcttatatc ttccgttgtt
     3361 ttgggaatct tgtgcggact tttttttaat tttcctataa attcaaacat cataactata
     3421 cttcttatga tactggtttt ctgcgtgggg ctggatatag gttctcaaca ggaaatagga
     3481 aagaagataa gaagcaagat aaaaacaata ttaatacaga cggcgctgac tatatgcgga
     3541 agtcttttgt ttgcaggaat ttccggtttt tttacgggac tttcttttaa agaagcggcg
     3601 ggagcaggtg caggaatggg atggtactca ctttccggtg tcatgataag tgatctttac
     3661 tcggcagagc tgggagcagt ttcttttact gccaacgtca taagagaagt tcttgctatt
     3721 gtcactattc ctcttttttc aaaagtttca gaactggggg caatatcggt aggcggagca
     3781 actactatgg atacaatgtt gggagttgtt tccaaaagca cctcggcaga aaatacgctc
     3841 gttgcttttg gacagggagt gcttttatct atggcagtgc ctgttttaat tacgcttatt
     3901 ttcagttgat gttaatgaat tttgcagtag aagttatagt ttgttttata aaccatcaaa
     3961 agaagtttta aatcagtatc atcatctaca ataatcagaa aacgccatta ataatgggaa
     4021 accatatttc aatttctcgc atgtataaga tagaataatt attggcagat agtaaacttg
     4081 cctaacttat atcttagcga gggggtaaaa aatgcttaaa aagagtatag cattattatt
     4141 ggtctttgta tcaataatat ctttgtcatt tgcctgtacc ataatagctg tcggaaaaga
     4201 cgctatggca gatggatcaa ccattataac ccacagttgt gattcagggt cggacaatcc
     4261 gcttctttgg attattcccg aggcagactg ggcagaaggg tcagtaagag agatttatca
     4321 gaacgctcat tatttctatt ttaaaggaaa caaaaacggt actcctgtct acaacaagga
     4381 cggttatctt accgtagatg aaaccgttgc taagaaagta ggagagattc ctcaggtttc
     4441 tcatacctac aggtacttct actgcggata ctcttttatg aatgaaaaag gagtagccat
     4501 aggagagtca acattctcac agggaaatta tgctaccgat accggaaagc agattatgaa
     4561 agatcttctt gaggatacat ggggcataat agactgtttt gccgctcagg atatagctct
     4621 tgaaagggca agcacagcca gggaagccgt tcagataatg ggtgatctgg tcaacaagta
     4681 cggatggtac ggagcaggcg aaataatgga tatcacagat ggaaacgaag tctgggtagc
     4741 cgagttctat ggaagagatc tttgggcagc cgtgagagta ccggatgaca gtgtttttgt
     4801 tggagccaac gtcggaatcc tcagagatct tgacctgaca gataccaaaa acgtcatgta
     4861 ctcaccaaac cttgtaaagt ttgccgttga aaaaggctgg tatactccaa aggaaggcga
     4921 aaaactcttc agcccgggtc ctgtttatgc accaagaaca ggcatgaatg taagagagtg
     4981 gagagcaaca gatcttcttg caccgtcttt aaaggttgaa tacaatcaga catactatcc
     5041 tctttttgta aagcccgata aaaagcttac cgttcaggac atatttacta tacacggaga
     5101 tgtttacgaa ggaacagacc ttgacctgac aaaaggaata ggagcaggtc ctttcggaga
     5161 tccttttgca agctacagga tcggcggtag ggcaagacct ataggagttc cgcagtcaac
     5221 ttacattcat ataggtcagg taaaaggctg gctcccagat gaaataaaag gaataagctg
     5281 gtttggttac ggtgctggtg ctacgacata tcttactcct ctttggccga taatggaaaa
     5341 gcttcccgat ttttatatgg tcggttccag atacgagccg ttaagaagag attccgcatt
     5401 ctggaccaac ctttatgtcc aggaagtagt aagacacaga tatatggatg ccataaaaga
     5461 tctcagagcc tacagagatc ctaagatgca gagcatatat actcaggcgg acaatgttca
     5521 gaatcaggct gcagcattta taagaagcgg caacaaagaa cagggaataa agcttgtaag
     5581 tgattttgct tacgatacag ccattgcatg gcagagaaac tggcttgacc ttggcgatac
     5641 aatatttgca aagtatgtaa tggacagaat atatatgaaa ggcccgtctt atcctaagac
     5701 atggcagaca accataactc ctgtaaaata aagtaaaaca ccggcgcaag ccggtgttta
     5761 ttttgttttt ttatagttgt acggttctat gtccgtgtgt cccgagcgct gctcctctga
     5821 gtcaaagttt atgaagtatg attctttctc attggtaata agttcaagtg actcttttat
     5881 ttcaagctcc ggtttaaagg tatactttaa aagcttacgc catgattttt tactaagaac
     5941 tgttgatatg taagttatat ccgaattttt agctgcaatc agggcaggcc cttttttaag
     6001 gggtgatgat tcccatgttc cggtctgttt tccttcaata acttctaatt cttcaatcca
     6061 catatctgca acaaggccat gatccttgta atcgatgtac tcttcgtata tggctccgaa
     6121 atcctttatt gtaaagctca ggtcttttat cttccatcca agaggtctgt cagaaataat
     6181 agagttattc tcatccttta tgttgctcat acaggttata aagagcgagc cctgatactt
     6241 ttcgagtttt ttcataaagt tctttggaat ataaagagca tatggcacta cgactatctc
     6301 gtaattttcg attttagagt ctctgaagat aaaatcaaca aagtatccaa agtatctgaa
     6361 tgccgaatat atatcctgca aagccttaac gtagtcaaaa tctttgcata cgccgtttat
     6421 tttgtgcatc catgcaactt cataatcaaa gtatatagct accttggcct tgggtcttga
     6481 agaaagctta ttaacttctt cagaaagttt ttaactacct tcagccgtgg actggtatca
     6541 aaatttcctt cgtagtttat cagaccgtta tggtactgct ctgcaccgaa ggaaacagct
     6601 ctgtctctga aatatgaaag gttgtcagct ccgtgtaaaa atgcctgtat ggttgaaggt
     6661 acaagccatt tagcaggaaa aaacaaattt ctgttctgcc agttaaccct gcctggctgt
     6721 tgctccatga cagtaaagtt ctttcttttc agagaccaca tgaggtcaag gttgaatgcc
     6781 tgctgaagcg ggtcaaactt aggtgaagga ttatagttgt caaatgagat gaaatcataa
     6841 agttcctgat gagaacagta gtccatgtct gtgaaattta ccatgaagtt ggttgtgact
     6901 ggtttatctg agtgctttct tattatatct atctgcatct gtgcaaattt tctggcggat
     6961 atacttgaaa atctataaaa gtcaagcatc tgatgaggat taggaatagc cggaatcttt
     7021 tttggggtgg tgatctgaga aaaactgttg tatatctgtg accaaaagac cggttcccca
     7081 tgctttgtta agcgagccga tatctccgta cttatcttcg aggtaggtcc tgaagttctc
     7141 atcgcatttt tcgcagaaac agtgggaagt atcttcacag ccgaactcat tgtctatctg
     7201 ccatgcataa agagcatcat gggaagaaaa ttcacaggcc agcagttcaa ctattttaca
     7261 tacataaagg gcatattctt tgctgttgta gcagtaatga cgtcttccgc cgaagttttt
     7321 tgtgtctgat acgatgtctt tctgaagtat ttgcggatac tttgatatga gccatgcagg
     7381 gggtgttgca gaaggtgtgg ataaaataac ctttatttgc agagctttgc agatgtccat
     7441 ggtttttttc agtagtgaga ggttgtactt cccttcttcc ggttcaagaa cactccacat
     7501 aaactctcct atgcggacaa actctatgcc gttatttctg aaagccgaaa ggtctttttc
     7561 tatacggtcc tcttcccaat gttcagggta ataagctgta ccaagcatct ttcatacctc
     7621 cttggaatat taaaaactct agattccggc gtttataatc gaatagaact tcttaatcca
     7681 aattaataca caattaaatt attctcctat tatattttag cagatttata aaaataaaat
     7741 gcaacgaaaa gataaatatt accaaaagta acttttttaa gaaattttaa gatatattaa
     7801 gagagtttaa tattaaaatt tatccaaaag atcttatgct atattttctg aagagttgct
     7861 ataatatctt tatggggtga ttaatatggt tgaaaaggaa agagtatctt tatgcaacga
     7921 taaagaagta aaaaacggaa agtatgttct ttactggatg caggcaagtc agagagccca
     7981 tttcaatccg gcgcttgagt atgctgtcct tgttgcaaat accatgaata aaccccttat
     8041 agttcttttt tgtataatgc cggagtatct tacggcatcc tttcgtcatt atcaatttat
     8101 gatagaggga ttgcaggaaa ctgcccaaac aataagggga tacggtgcgg cttttataat
     8161 aaaagcagga gatccatgtg aaattattaa agaactttct gaaagctcat caatgattgt
     8221 gttcgatttt gcatatcttg attttatgaa agctgtaaga aagaagattt ctgcacagac
     8281 tgcatgcagg gtagtacagg ttgaaggcaa tgtggttgtt cctgttgctg ctgcaagttc
     8341 aaaagaagag tattcagccg caacgctaag accaaaagta agtgcggtta tttcaaagta
     8401 tcttaattct gaagaggaaa taacagagct aaaaaacaaa ataagcgatc cacagataga
     8461 atcgcttact gaacaacaag caaaagagta cataaaaaag ttcagaacaa acaacagata
     8521 ctttgatgaa agccgcttta taggaggata ctctcacgct cgtcaaaagc ttgacgactt
     8581 tattaaatat aagctccata attacgcaga tatgaaaaac gatccgacca gcgaggttca
     8641 gtccgattta agtccgtatc ttcattttgg acagatatct cctgtttata tagcaaatca
     8701 aattacgaaa gtactttcag agtcagccca agagtatttg gaagagctta ttgttagaag
     8761 agagcttgct cacaactttg tcatgtacaa cgatgattac gctcagtata actctgttcc
     8821 agcatgggct aaaaaaacct tggcagaaca tctttcagat cccagagagt attactattc
     8881 accacaacag ctggaaaatg cacagacgca cgatgagtac tggaacagtg cacagaatga
     8941 gctgctgctg acaggaaaaa tgcacaacta catgagaatg tactggggta aaaaaataat
     9001 agaatggagc gagacacctc aaaaagcttt tgaaacaatg gtctatctca atgaccgcta
     9061 tgaaatggac ggaagggatc cttccgggta tgcaggcata tgctggtgct ttggcaagca
     9121 cgataggcct tggtctccaa ggagtatatt tggtaacgtc agatacatga acgataaagg
     9181 acttgaaaga aaattcaaga ttcaaaagta cgttcagagg attaaagacc tcaaaaaagg
     9241 aagctaattc agtctatctc ttttatttta tcctctatta tgcccatatg ctcttttata
     9301 tactccagat attcctgtgc tacaccgttc tgataacggt cacacagatt atcgataatc
     9361 ctggacgaat agaaaacaat atttatcata ttgtcgtcca tcaaaagtct gccttttaca
     9421 cacgcatcaa gtataagttc ggtctttgcg gagattattc tcaccatttc agcacttaca
     9481 aaaccggcaa gtccctttat agtgtgaaaa gacctgaaca gggcaattac actttcctga
     9541 tctgcctgac tgtcagaaga tatgatcctg agagtttcac gtattacagc cgatgattcc
     9601 cttgtttccg aaaaaaaagt ttttatgaaa tccttctgta tcttatcaag tacctcggta
     9661 tttacaccgt ttacggtatc aatcaaagct ttttctgttt ccagtctgtt tatgaggctt
     9721 aattcccgca tgtaacgcct gtctgacata agatcagcct ccctttaaaa gacccagctc
     9781 ctggagtttt aaaataaagt cttttttcaa aataggcttg attatatatg cttcacattg
     9841 tgccttgaat gaattgcgca cattttccac atcgctcagg gcggtggtca tgataatctt
     9901 tgtcccatca agcccgtata ctccgttttt gttttctgca gaacgtatct tttgcagcac
     9961 ctgtatgcca tccatctgtg gcatcatgat gtcaagacat accaggtcat aacgtttatt
    10021 tgttttgaga gaactttcaa aaacacttat tccctgtgcc ccgtcgtttg caatgtcgca
    10081 gacggcatan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng ggcagccaaa
    10201 tctgaaaaaa tttaaatact cctcaatgcc tttaagatca agcagtttct ctgttgcctg
    10261 ggtgcaaaga atatcatagc ggtgttctct ctgtgcacct gtagttatct ctgttccaca
    10321 gtatataagg tcaaaacttt tagttatctg tttattttcg ctgcgcatat gataaaaagg
    10381 ccgtacactc catggaaagt ctgtcaggaa aacaaaatct gaattaaaaa cgtcctttac
    10441 atatcttccc aatgcttttt caccgtccga gttcaagtca gaattttcca agcattctat
    10501 gccatagctt ttaacaatgc cttgagcctg tgtcatgctt attctcggaa aaggtagctc
    10561 cggaatattt atctgtatac cgtaaagttc ttcaagttgt ttgctgtaaa gctctttaag
    10621 acctgaaaat gtaaacgcaa gccactgttc ctcaaaacac ataacttctt caacagtttc
    10681 tacgtatgcg atttccacat ccagagaggt aaactctgta gtgtggcggc tcgtaaaaga
    10741 tggttctgcc ctaaacaccg gacctatctc aaaaactccc tcaaaacctc cggcaatagc
    10801 catctgttta taaaactgcg gagattgggc gaggaatgcc ttacgttcaa agtagttcac
    10861 ctgaaaaacc tgagcacctg attccgaggc tgctgccatt agcttagatg aagatatttc
    10921 tgtaagatgt ctggcgtaaa aatactctct tgagagtttt agaaaatctg aaaaaattct
    10981 gaatataagc ttcttttttg ggtttctcag atcaagatgc ctccagttaa ggcgtttttc
    11041 aagagatgat tcgagttctt cagatatatc tataggcaga ccggtatggg ctgatgaaag
    11101 cacctccaag tcagtaacca aaacttctgt tccgcttttg ctgagatctg ttctgttgag
    11161 ggtgccagaa attttaagca cgctgttgac agaaattttg tccaaagcat ctattttttc
    11221 tccgagaaga tttctcagac atgttgcctg tatctgtgac gaaccgtcgt caataatcat
    11281 aaacttcagg tttttacctt tgtttctaac tgtttttata aagccgcata tacatacctg
    11341 tgcttcagac tttaagccct gcagttcttt tattctctgt ctcataaagt acacctccgt
    11401 tttaaaatag aaaaaccggt gctggctaaa taacaaaaac cagcaccggt taattaatca
    11461 aagattaatt aaggcgctat gatatcatca tcaacttttt tgaaagaaag tacactcatc
    11521 tttgcctcca aaataattct ttaaaagata ttatagcata ctccgcaata aaaatcaaaa
    11581 agaaagaaca acttttagga aaagtattaa gaagtatatt atagctgtca cgattccaat
    11641 tgagaaaatg gttttgagaa caccctgcct gtaaaaatca ttggcgataa gccctggaat
    11701 tatggttcct atagttgtag atatataatc tacagggagg ttccgtatag tgatataatc
    11761 aatcagcagt tttacaagta tacctgtcag aatatttact gccagacgtt tttttccgta
    11821 aaggataaga aatctgttca tcagtaggac aagaagatag gttataaggc ctgagcccaa
    11881 ggtatatagt attcttaatg gattgtttag gaacatataa aagtaaaaag gggttactat
    11941 gcctcctgcc gaaagtccgg taagccacca gtagagagta cttatggaaa ttcctacgga
    12001 aaatgtttcg ggattcatcg ttccacctcc agggtcataa gccatgctga aatatcgcgt
    12061 atattaccga atccgaagac aagagagttt tggggaataa gctctgatat ctttctgaca
    12121 tcttttataa cagtatactc tatgttttta cccatctttg aaggaagcct gtgagtggat
    12181 aaaagctttt taaatctacc gtctgcaaga aagctttcaa agcataatgt cctatcctgt
    12241 ctgtcatccc gcggattaaa annnnnnnnn nacctatcat agttttatcg gggtatattt
    12301 ttttagagta ttcaaaaagt ttttccgttg aaagaagatc attggcagca aatgcattgg
    12361 ccaggataac agaattactt attttaagaa atttaaagga gcctacctcc ggagaaactt
    12421 ctttcatgcc ttcgtatgaa gcatcaagat ctccgccaag cagttccact gttttaagcg
    12481 ccgttcgtat attatcttca aatacattga acttgaaatc atttttttgc acagggaagc
    12541 tatcatcttt ttcatagagt tgaagatcct ttctgacagc ctgaacatca aggaaaagct
    12601 ttccctctgg agagatatcc cccacaacaa ctctcgagcc ttgcggaata cttaaaagaa
    12661 gggttttcaa ggtctgtacc tcgttttcac ctatttcctg gacgtgatcg gtgtaggcat
    12721 tagttaatat ggttatatca ggctggacta ttctagcaca cgcactctga aattcgggat
    12781 ttactgccat gcactctgcg acaacagcat tgcacttcat tttttttgct ttataaataa
    12841 gcttgtactg ttcctgaatg ttggcaggtg cgattctatt aatcttttta aatgaacctc
    12901 ccggaagtat ccaatagggt actgttcctg tagttttgcc aagaactctg tagttcatag
    12961 ctgtgagagc tgcatgtaca agccttacag tttcgctttt tcctctggtt ccgtttactt
    13021 gaatccttat aggaatagtt tttttgcaca gatataaacg gatattttca tatacaataa
    13081 ctactataac gataagaaat gcagtaagta tatagttcat aatttttcac cgcttctttt
    13141 tttagctttt ttttccctgt atcctgtgat aagaataaaa attgtaagtc ctgaaaatat
    13201 tgcaagataa tccatcctgt atttaaaatg gaaaaaaaca ccgcttgttc ccaactgtgg
    13261 aataggaatg ggatcaacag ggattttgtt tttcatggcc aggtctttta tattcagtaa
    13321 atgaattaca ggaatgccag actgagaata ctcgaatata agtcctttga cagggcttga
    13381 aagaacacgt ggcgggctct ttgaaagacc gttgggaaag ataagagatg cctctgttgc
    13441 tccgaagttg gtggaagccc ctcctatatt tatgaatacc ttcatcgggt ttgaacccaa
    13501 tgcaccggca taaagttcca ttctcttttt aatgcttttt tcaagagtgg cttcttttat
    13561 tatagtaact gcggctgatt gggcaatttg atcaaagaca gcttcagatt cttcaaaaaa
    13621 catatactgt gcaagatcat gatctcctcc aagggatact gcaagaagct tgtaatcaaa
    13681 gatattctgt ctgttaagct ctctgagcat atcgatgaag gtaaaaccag gtatcgttgc
    13741 accgtaattt gaagcaccta ttgaataaat tataagtggt ttaagctcca tagcttgggc
    13801 agcactgaga gaggcaatta aaagagcagg aaatgaaccg cttgccccta tggcgatatg
    13861 atcacctgcc ttaagatcaa gctctttaaa gaatttaacc ataagcgcag caaaatctgg
    13921 atttgtagag gttcttttgg cctgaagatt acctactgaa gttaccagaa gagtcatatc
    13981 ttcgcctatt attcctgtcc ggtttggatc gtcgtctatg tttatagata tattattttc
    14041 tatgcgatag tcttttaaaa cagatataga gttttgcatt atatgagaag ctttatactg
    14101 aagatcgtaa tagtcattct tggtgcttac ctttgttttt tcaataaaac aaacaccaaa
    14161 aacagatata acgaaagcca ggaacagata aaaaaaaggt atggttgatt tagtaaatga
    14221 gtttatagga gtcacctgcc tatatagttt atagaaatta tacctttggt tattggcata
    14281 tttaatgaga ttttgtgaca ttttaaaaaa gacatgccgg attgctccgg catgcttatg
    14341 attcaaatat tcagattatc tctgaggagt tgtgaaacct attgtgtcgt tccatgtctg
    14401 aggataagga gtggttctga agtttatata acccatagca tatctgccga gtaaataatc
    14461 tccaagtttc agccattctg tattccatgc gacagcattc atgtagccgt agttatcgat
    14521 aagagcaact gcttctttgg tcttaccctg tctgtaaagc tgacctgcga gttcctgaac
    14581 ctgtggggta actttgtaga gcatatccat cttaggagct ctgaggtttc tgatgtcttt
    14641 tatagcttcg ttgtagtgaa gttcagccat ctgctgtaca tatgtgttta cccaccagcc
    14701 ggaatctctt ctgaatcctt cgtatctgct tccgatcgtg tagaagtcag gtaacttggt
    14761 cattgctgcc caaagaggtg tgagatatgt tgtatcaggt gcaccgtaac cgtaccagct
    14821 aactcccttt ataggatcag gcagccatgc tttggtctgg ccaatgtgca ggtaacatgt
    14881 tctgtgcata ttgattgatc tttcccatgt agctgttttt tcggttttgg aagagttggc
    14941 gtatctgaga ggatctcccc atggaccagc agctgctccc ttggtaagat cataaggagt
    15001 accttcgtag taatcggcct ttaccttcca tatatcgtct attgtaagag ctttttccgg
    15061 aactacccaa agaggatatc tgagttcgct tggaccatag ttgttggcct tcatgtaatt
    15121 aggagcaaca aggtcaaggg ctctccattc tcttcttgtg gcgtatagac tgtcatttgg
    15181 tgagtaaatc tctgcaagtt tccactggtt aagaggaagg tcttttgaaa cccaaccctg
    15241 ctcaacagca gttttgaaaa ggttctttga gtacattacg ttagctgtat cgttaggatc
    15301 gacttccata attcttaatc tgtttgctgt aactgagaat gcattgtcag ggattctgac
    15361 tgcaacccag aggtctgctc cgtagtactc agcaagccat acatctttac cgtctccgat
    15421 aaacattgac tcaggaccgc cgcctacatt gctccaacca tatgtttcaa cgaggtctcc
    15481 cataaccttg acagcttctc ttgcactagc tgatctctca agagctatgt cctgtgcgta
    15541 ccagcagtca atgattcctt cgttgtccga gaagtactta gcaacttttt taccatactc
    15601 tgttgaggta tcaattccac ctgttgattc tgatattgca acaccctttt cattcatgaa
    15661 agagtatctg gaatgaaagt atctgtaggt atgtgcaacc tggggcatct gaccgacaac
    15721 catggcattt ccgtttctgt tcttggtgta gtctactgat ttcggaaagt ttgagaaatc
    15781 aatatagtcg tggctgtcaa gaactatgtc tctcatggat ccttctgacc aatctgcttc
    15841 aggaattatc cagagtctga aatctgcact tgttgaatcg tcgttgtgcg ttataaccgt
    15901 tgaaccgtca gccattgctt tgttacctgc accaactatt gtacatgcgt acgaaatagt
    15961 tgtagcaagc atgacaacta ctaacatcat aagaactaac cagctttttc tcatgcttat
    16021 acctcccctg taatgtgtgt tactaagaac gcgaagtggc atggttttga ccatgcctgc
    16081 cccgttctat taaaattagt atccgtactt ggaaaattca ggatttttgc ttggagttaa
    16141 ccaatctttc caccactgtg gatatccact tgaactaaag cctattcttt cagcagcgta
    16201 ctttgtgaag agcctgtcac cgagttcgag ccagtctctc tgccatgtta ctgctgtgtt
    16261 gtagcagaag tcgctcataa gcttaactgc gcctttggta tctccgtttc tgtacatagt
    16321 ggcagctgct tcctgaagta ctgatgtctg ggtgtatacg ctctgcatct tcggatctct
    16381 gagagctctg aggtccttta tagcttctga gtatctcaga ccgaccatct gctgtacata
    16441 tgtgttggtc caccagccgg agtctcttct gaatgtttcg tatcttgagc ctactgcata
    16501 gaaatcagga agtttttcca tagccgggaa gagaggagta atatatgtgt gtccgaccga
    16561 accgtaaccg taccagctga ttcctctgaa ctctcttggc aaccatgctt taacctgtcc
    16621 gatatggacg taagctgtaa gaggaatacc tataggtctc tgtcttgaag caagaccgta
    16681 gttagtcaaa ggatctccaa aaggtcctgc agcaggtccc tttgtaaggt cgtactgtgt
    16741 gccttcaaag tgatcgcctg atattttgaa aatatcgtct actgataaaa gcttgtctgg
    16801 ttttacgaaa agaggatact cttttgcatt gtcttcaatc ttcatcgaag gggcaacgag
    16861 gtcgaatact ctccattctc tcttgttatg tgcatttctg tttgcataaa cttcagcagg
    16921 tctgaacttt ttacctgagt tcggatcata ccagcctttg tttatagcat attcaacaaa
    16981 accgggtgaa gtgagaactt catacttaac gcctgaaact gggtctatac cgtctcttgg
    17041 gtcaacaaga attctcatgg tgtttgcgcc aacataaacc atatcgtctg gcatctttac
    17101 agccatccaa aggtttgcgc cgtagaactc cataatccat acttctttac cgtcggtgat
    17161 gtccattaac tctccgccgc cgccggcaac gctccaaccg tacttgttaa tgagttctcc
    17221 cattacctga actgcttctc ttgcggttgt tgctctttca agagctatat cctgtgccgc
    17281 ccagcagtca acaattcctt cctcatcttc aaagagggcc tgtcttacgg cttttccata
    17341 attattgctt gtagttatac cggcagttga ttcgccgata gctactcccc attcattcat
    17401 gaaggagtat ctggagtgga agtacttgta ggtctgtgcg ggctgtgggt cgatacccat
    17461 gaccatggct cttccgtttc tgttcttgtt ataatctaca tttggaaaat tggagtaatc
    17521 aatatagtcg tgtgaatcaa gaactatatc tcttactgtt cctgactgcc attctccgcc
    17581 aggaagaatc cacaatctga aatcggcaac cgaagaatcg tcgttatgag ttattattgc
    17641 cgaaccgtca actgtggctt tgtttcccac tgcaatgatt gtacaggtga aagctacagt
    17701 gctaactaaa agaacgataa gcgtaaaaag agctaccagt atcctttttt tacccatgct
    17761 atatgcaccc ccgtaggttt ttgagtagag acccaattag aaataaaaaa attattggta
    17821 gaatactctg gatggatcgg aagttgcagg atcgtggagg aaatctccaa gagctgcgtt
    17881 tcctttggtt gttgcgggtt ccattatatc atgaaagccc ggaaagtcaa gaacgagctc
    17941 tttgtcagga tagagcatgc tcatctgttt tatggcttcc ttggtgcctt cgaggtgcct
    18001 ggcgactctg tagtacatag gagcgccctt tacaacgtat ccgcccctgc cgtcttcatc
    18061 gataggcata gttatgcctt ctcttgaatc atatcttgcg aaaagaagct tcttatctgc
    18121 tgctgtctgt atgaactcgt cgtttccttc catgagaaga tcaacggtta ctcttcctgc
    18181 aactctttct atgaaaggtt caagagtttc cataagaaga acaaaggtat gatgggacga
    18241 tttgtttata acttcaagtt ttttatcata atcataggca tagcctgaca tttcaagctt
    18301 tacaagctct ggaacaagct cttcgtctat ggcatagtct cctacttctc tgtgtgaaag
    18361 acctctgagt tttttaggag aagcttcggc cttcatggga aacttgtatg tatcaagagt
    18421 catatatgcc agcattgata tatcatctgc aaagttatgt gcaacgtatg tgtcgacaac
    18481 cggatacatg agcgatgctt cgtgatagtc aaagtatatg tctacttttt cttttctcat
    18541 aagctccatg aaagcatagg aaattctttc ggtaagtgtt ccgtctgctc ttcctggcca
    18601 tgttctgttg aagttcctca tatcctggta ggtcaggttc tgtcctgaag gatagtgaac
    18661 atatgtgaaa ggatcaggcc actggtcaag tgggtttgtg tttctgtcac cgatcttgta
    18721 ctgcttagga ccaaatgctg tatctatagt atagaacatc ggataagcat ttccaagcat
    18781 accaagggtg ttggcgcttc tgttggcaac aggtacaaca aagacctttc ctttttcgac
    18841 tttcatgttt tcggcaagta tgtatcctgc catcattgta gcaggttcat aaggatgggt
    18901 tccacccatg agaagtgcca ctcctccggg aactcctgaa tcatatacat atatcggggt
    18961 atcgccccat gtgcctttaa gactcgggag gtaatcgctt agcattattt gctttgtaag
    19021 accttccgaa gctactaatg attccttgta atgtctaaga ttccaaaaat ccattcctgc
    19081 aattattccc attactccta tcaaaaccaa aactataagc ttgaaccaga caggtgcact
    19141 tttattctct ttattcacag ttattcagct ccttacttta tatgaaggac tctgagtata
    19201 aaagtcacga ttacatatgc atagacaata tcgtataccc agccttgtct cttatttctt
    19261 ttaaaaaggt tttttaccag aaacaaacag attattgctg ccagaacaaa gtaaagctga
    19321 tggattatct cctggattat caacatatta tcatacctcc cctatcaaga aataacaagg
    19381 aacttgaatt gattcgggaa aataaacatt atcaaagcta cggctgccat gaaaagaata
    19441 ggaacaaggg cagacttaag gaatgacata tagccttcct cgtacttaac cgattcaatg
    19501 cttaatcttc cgacaattct ggaaggaggc agacagtcgc ctatcgggaa aataagactg
    19561 agagcggctg taacaactgt aacgttgaaa ccgaggttat tgaaaaggaa tattatagga
    19621 gtaccgagta taacagcgct tccgtagctc aatgatccct gtgcaaaagg accgaataca
    19681 agaaccaatg cgtatatcag ataaacagga agggttacga aggttatagc tataagtcct
    19741 tttacgccag ttgcgttcat tacgttgaca agtgcaccaa cacttataac tgtagcaaga
    19801 agcgggaata cctgttccat tgtgttactc ataacttcaa gccatttttt gaaagtagtc
    19861 ttctttggat tgcagagaag agcgactact gtacagagta caaaggtcaa aggcaaaccg
    19921 aatatcggtg tggagaatgc cgcatacttc tgaagaagta tcaggatgat cagtgccaca
    19981 agcggaagaa gtatcctgaa ccagttcatt ccgggaactt ctggtggcag actggcgaga
    20041 atctcttctt ttccctttgg ctttgctcct ctgccgtacc atattatggt aaatatactt
    20101 attacagcta taggagcgag aagaacaacg tcaaatccta cgtaaggcat atttgcctgg
    20161 gcggtcatca acatggccca aagatttata ggaggtgcag ccgctgcgag cattgcaaac
    20221 atgtacataa atccgactat ctgttttttt gaaagcccca tataagtgag aacggttgtt
    20281 acaagtcctc cgacaacaaa tatcgaaaca gaacctgctc ctgtcaatgc gcctgggatt
    20341 atcatgaata ttgccataaa gatcatcata atccatttgt ggttatagaa tctgtttacc
    20401 agctttctga caagtgcatc catggctccc gtttcgctgt atatgttaat gaaaagggaa
    20461 gcggtaac
//
LOCUS       DAOSHR010000010        20449 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_2121_length_20449_cov_5.868932, whole genome shotgun sequence.
ACCESSION   DAOSHR010000010 DAOSHR010000000
VERSION     DAOSHR010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 20449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20449
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2121_length_20449_cov_5.868932"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="PLU28_01080"
     CDS             complement(<1..385)
                     /locus_tag="PLU28_01080"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOG33688.1"
                     /translation="MINALALGSGGVKGIAHIAFISEIVRKRNIKFDIVTGCSAGSVV
                     GGVYALNPSKIEFLKDFEDIIKKNSQKFIDLSNALSSKVNSFTRLLSSRGIAQNDLVY
                     DILKQIYGKKRFSDCKIKFGVVAFDI"
     gene            complement(401..2521)
                     /locus_tag="PLU28_01085"
     CDS             complement(401..2521)
                     /locus_tag="PLU28_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG33689.1"
                     /translation="MRKFFLVIIFVFVSILTFSLEIRDLKPSSPYYSQVVRMVTDGLL
                     ELDSQGRFNGSSNVMRFDIAFFGANLIDYLDKKYSSSFDDFEKRISQIDTESLENRIN
                     NIETTLYNYDSKIISMEKRIDSKISGLNSDIAQQKSRIEALEKFMNIDGPLDTDSAIF
                     KVISDNAVAIANDTAKKTADSYLKTLMSFSEQSNKKVDLFQSELLRIEEKLDKTTDSI
                     SFLFAQYEEKNRQQLNDYAKQRLDSEMEGMKVALRNIVNSHIGFYNQTAEATLSTLRD
                     RIAALEYSGQNQYEKRFNDIISRLVYLESKETVPAFQSTVQNDKELLIIKKDTQSLAN
                     DIKDTKTLYDNLNNRVEYINAFIDTYDGRNEYFTKKLSELESKNSDVNSFVGELRQML
                     ENIKATSDQSIPSSITDYVLKNDFVNLQQEVRLMQEYLNMYSDKLSQLYIYTIKANEY
                     DEKFKISEKTQSAQNESIQKLSLRTDSVESQINSLSSLIKLDKDTLNTIGNVASIGKR
                     VGENEKKLEDLSSLVYENQNKLELIYNSDLLKQSNSLVSEINKKEIQKLKEDYDFLVK
                     SYILLKNQTVSPETTKIAETVDLSNMKTEIKRELTSDLASELIKLQSDMSDTKKTVLS
                     LSSKYNEMNAEKASKKEVADIELSVQKVTASSELSLKITEMEKQISELQKPNQDIFWS
                     STISGVLGVAVGALITYFLLSPSL"
     gene            complement(2536..4215)
                     /locus_tag="PLU28_01090"
     CDS             complement(2536..4215)
                     /locus_tag="PLU28_01090"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOG33690.1"
                     /translation="MGNIGKRLFLTVFILLAYILSFSFTLTLSGLEGSSVFIDGIFKK
                     IIEKSVYNIDLSDTTHKLEVKKPGYSDYSLDISGTKGSKLEISALQVPLSILNIKTNS
                     DSFYINYMFNNNNIRQKVINDTQTYLPSTIRELKIEQAGYLSQTVTIDLKPFYENKLS
                     VTLKRTDFAYISSIPSDASIFVGSKNLGTTPYATALTLLEGKDIILKAPGYMDFSFKT
                     DSKTNDYIFTMTKACAVLFSSEPSGALVTLNNEYLGITPFTAQIPMGNHLFTVSKALY
                     TPKKLSANISEATPQISYTVSLEEDIRTFKLLNAENAEVFLDGINLGSGISFIVADKK
                     EHFLQIYSKNTSDLLGIKLDQKAPKIIDINKITVMNLYSSEKKSISYQNEISTVPAQF
                     IANIISKTQTFQFESLTKKYNISAESGKTNVLIIDSPGYGFVNIINNVDGTLIYIDNQ
                     LIGYFGVFGYPLSTGRHTVTVKYQNIEKTEKIDIADQEVKNLNFIFEDKIPIRPYSSG
                     EYKIDGVIYPPSQFIVYVSKGPHVIETKGIKFVIYIDAAQSLFIDDLAVLK"
     gene            complement(4199..4642)
                     /locus_tag="PLU28_01095"
     CDS             complement(4199..4642)
                     /locus_tag="PLU28_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOG33691.1"
                     /translation="MYKNAENMESILRSICFHIRAAGRKVLKDYDISPAQFDVLQLLY
                     FDGEKKLSEISKNLGVTKSTTTGLIKRLEILYFLDREQSEEDKRVYRIKLTDSGRELI
                     TKVIKKRVEVMQSVIDRLGNEKELIEKLSELQKTIKEVVKSGEYR"
     gene            4755..5231
                     /locus_tag="PLU28_01100"
     CDS             4755..5231
                     /locus_tag="PLU28_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG33692.1"
                     /translation="MSKNITITAMLSVLTIILFSCQMFIPVVGIFVAYFSIIPLMFIY
                     EMTNAKYFFMSLITCISMVMILNDPFGWIFFDLLLVPPAITTVTKNKILKLIMIVPIE
                     IGFLLLFKILVVNGSKIDMILSNIWYILGILFYFAGTFFYRKAFLISKMLLKKINY"
     gene            5295..6941
                     /gene="argS"
                     /locus_tag="PLU28_01105"
     CDS             5295..6941
                     /gene="argS"
                     /locus_tag="PLU28_01105"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOG33693.1"
                     /translation="MSILKTIQKIIEKVLADMGISQSLDFKVEVPPQDDLGDFSTNAA
                     FLCSKILRKSPKVVADEISEKLRQLDTFVRVDNINGFINMFLDHSVYQMACAGIISNP
                     RNIGKSNIGNGENIQFEFVSPNPTGPLTIAHGRQAVIGDVLCNIFDESGYNVQRELYI
                     NDAGQQIEYLSKSVWIRYNELFGIKHELPEDGYHGEYIIDVAKEFKNEYGDKYKDIWN
                     EEVNDFFKEYSLKKMLSMIKQTLKRLGVNFDVYFSEKLLIKEKIVSRILDTLEKSDST
                     YAKDDAVWFKVSKYIDENDKVLIRSTDNRATYFCDDIAYHYYKYHRGFTKVYDIWGAD
                     HQGHIPRMKASMKALGIPDDFFNVILHQFVTLKKGDEVVRMSKRAGNFVTLDDLIEEV
                     GTDATRYFFAMIDPDTPMNFDLELAVKKSSDNPVFYVQYAHARISSILKQAEERKIDF
                     KMFEGLSEVKEKEELLLIKELSLFSNVVAEAAKNRKTNFITTYLEKVSSRFHTFYTKH
                     SILNTDNSQLIQARLNICLATKIILERGLALLGVSAPDKM"
     gene            6954..7157
                     /locus_tag="PLU28_01110"
     CDS             6954..7157
                     /locus_tag="PLU28_01110"
                     /inference="COORDINATES: protein motif:HMM:NF014304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional coactivator p15/PC4 family
                     protein"
                     /protein_id="HOG33694.1"
                     /translation="MYEIQRNPTEKLIIQKNEFKGKEYIDIRIFYEDEGEYKPTKKGV
                     TFNPDQIDEVIKALESIKEESQK"
     gene            7329..7958
                     /locus_tag="PLU28_01115"
     CDS             7329..7958
                     /locus_tag="PLU28_01115"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOG33695.1"
                     /translation="MLDNKGFDLWANGYDKEVGISDEENRYPFAGYKSVLSKIYHKVL
                     EKNKPSVLDIGFGTGTLTAKLYEQGCKIYGQDFSVKMLEIASKKMAKAKFYQGDFSLG
                     LVKPLRKYTYDFIIATYSLHHLADKEKIVFLRELKELLNGDGVILVGDIAFENRIELE
                     NCCNEAGSEWDDDEVYFVVDELKKELPELIFEKASYCAGVLYFGQNTLF"
     gene            complement(8140..8349)
                     /locus_tag="PLU28_01120"
     CDS             complement(8140..8349)
                     /locus_tag="PLU28_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG33696.1"
                     /translation="MRDVKQVNLYLDANLYEQIREFAARNHTTITSVLNQAMSFYLKN
                     AEEIKKFDEIENAENLKNFSSENAL"
     gene            8584..9129
                     /locus_tag="PLU28_01125"
     CDS             8584..9129
                     /locus_tag="PLU28_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG33697.1"
                     /translation="MKKVYLCMLLVFLVVSGFSDYIAIGEDGKIVQITQENQSIPLGL
                     KVVDKEFNRKFLDLLKGAETEVLYDAPIQIVSYQFERIMVDSSIKKRFSLSIKNTSYK
                     AVTSFSVKILAYDIYNYELPIDYKQDSTVTVERIILNPGDIYDNLNTMSFYFHDSIYA
                     VAPFVISVVYADGSTWQGGIR"
     gene            complement(9145..10332)
                     /locus_tag="PLU28_01130"
     CDS             complement(9145..10332)
                     /locus_tag="PLU28_01130"
                     /inference="COORDINATES: protein motif:HMM:NF024241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG33698.1"
                     /translation="MNYAAELKKNIQRNYLFTFLYNFNLTSSIWMIYLASKGMTLIEL
                     GMLESIFHITSFAMEVPTGAIADIFGRKVSRTLSRVFSVLYFLIILFSDSFFMYACAM
                     GICALSYTMESGAGEALVYDSLKENGNEKYFTKISGYNEVITQGAMFGAYILGGYLSN
                     FNYIYAYTGAIMIGVISILQSLSFKEPLIKSDSGSKNIKDVFKQAFESFRIVKKDKKL
                     LFVIIFVEAFLMFTTTLFFYLQNYFLSIGYDPLSIGFFMALSSIASATSAFFAHKLEK
                     IFDEKKLFAVLSIMSCFSIWAIAFSTVPYVFFILTNIADGVLYVVSSNYINKRIPSEN
                     RATILSFCSMVFSLYMMVLFPAFGVIGDKFSLKISFIFMGAFSTIITFILLKLLFSSR
                     KDG"
     gene            10487..11170
                     /locus_tag="PLU28_01135"
     CDS             10487..11170
                     /locus_tag="PLU28_01135"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOG33699.1"
                     /translation="MIDIYKVFIVEDNLKALKELEILLKRYGFEIVCAKDFSDIPLQI
                     QNVKPHLILLDINLPQYDGFFICQNIRAVSKVPIIIVTARDSDVDTIMGIRLGADDYI
                     KKPYSPEILVVKVQAVLRRVYEMASADESCMKYKGLFFDLKDYSLKYEDKTVELTKNE
                     VKILFLLMNKAGSIVTRDELIYELWNNDEFIDDNTLTVNITRLRKKFESLDSCVIIET
                     KKGVGYILK"
     gene            11167..12177
                     /locus_tag="PLU28_01140"
     CDS             11167..12177
                     /locus_tag="PLU28_01140"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOG33700.1"
                     /translation="MKFREYIKEYELFIFFELLGLIIIDIYLFLINTFFQLIIIIDII
                     YLLSPLLFMIYNFHKKKEYYSTIYRTMDNLEKKYFISEFMAQESFISLENEIMKDILN
                     TMFLSMYEDIKNSKEKQLDYQEFLEIWIHEVKNPLLTLKLIVANNKTKVNKSILEELE
                     KIENLLEQILYYSRYENLENDYVIKETLIEDFVKNAIKKHSRQIIENNIKIQLSNLDN
                     IVKTDSKWMEFVCSQIISNSIKYVKDNPEVKIFAFEDNNSVALVFEDNGIGIKSKDLS
                     RLFSKNFTGYNGRLEKGSTGFGLYIINRIVHKLGHEITVDSQEGSYTKIEVKFSKAVH
                     KW"
     gene            12297..13064
                     /locus_tag="PLU28_01145"
     CDS             12297..13064
                     /locus_tag="PLU28_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015567056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG33701.1"
                     /translation="MKNILKIEKLEKIYGLRGNVYKALDGVNFDVYEGEFLGIMGPSG
                     AGKSTLLNIISTIDKPTSGSVVINGQNIVNMKKNELSKFRRDKLGFVFQEYNLLDTLT
                     VKENIALPLSLANVSYREINRRVKEVSNNLEIVDILDKFPYEISGGQRQRAAAARAII
                     ADPALILADEPTGALDSRSSETLLSTLKNINENMNVTVLMVTHDPFAASYCKRILFIK
                     DGRIFNEIVKGEDERKKFFTRILDVLAVLEGDIGNVI"
     gene            13054..15039
                     /locus_tag="PLU28_01150"
     CDS             13054..15039
                     /locus_tag="PLU28_01150"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG33702.1"
                     /translation="MLFKLIKRNIRRSFRDYVIYFGTLILSIAIFYVFNSLDSQPAWS
                     GDEYPQAKIVISSLMGGLSFIISFIVGFLILYANNYIIKRRKKEIGLYMLLGMSQKKV
                     SFMLLSETIIIGFLSYVVGIIIGIFVSQIFSLITMKFFDIAINKIEFVLSYPAVIKTL
                     IFYGITFSLVAVFGSFGVSRFKLIDLLMGERKNEEFKIRKKAVVAIIFVLSCIDLFLT
                     YYFADYVASRLLVSPQSIFAIIFGGSLGTYGLFYSGFSMIFGVIKSMIKFYFSKINAF
                     LSRQLLNRFNTDMGMLATLTVLFTLTLSVFISGLGLKNYIESSVESNSPFSFNITVYE
                     NAEKIKQEFDEAAYRLGKDYLKDSLMVRSFRSDIRIKSISPDKEYFGNSELLRNYLND
                     NFYIMLQSDFNKLIKAKNIGEEELTGNEALLYVPDGSKDFKEYLENELSFNRKLFLGD
                     SQIEIKKIFPQHKIGNFMVFGSCALIIPDKFDYITQGYKTIWNIDFNFKTGYPSEIDK
                     EFQKISDQYELRMNTLKEAKANASMSRVLVIFVILYLGITLLIASAAVMALKELTGAI
                     DSKKNYQMLSKLGADDRLIKGTIKKHMRFYFFIPLLVAFFHNIFAVNFITKFLNAFNA
                     QNTFLITLIAMGVFLGVYLIYYYITSRMAIRIIVKEE"
     gene            complement(15094..15477)
                     /locus_tag="PLU28_01155"
     CDS             complement(15094..15477)
                     /locus_tag="PLU28_01155"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HOG33703.1"
                     /translation="MIKVALIAHDKKKIDLAMFVREWKDVFCKCELFCTKTTGSILKE
                     KIGLETIRTFESGPYGGDLQIGAMIVSGEIDFVIFLRDPLTAQPHEPDVSALLRVCDV
                     HNIPLATNLATSQAIVLEIQKKLNQ"
     gene            complement(15488..16492)
                     /locus_tag="PLU28_01160"
     CDS             complement(15488..16492)
                     /locus_tag="PLU28_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HOG33704.1"
                     /translation="MKELIKNITEIYSPSGREDEVRNFIISQIKDYVDDYKIDKIGNL
                     IAFKKGRSGKTILFDAHMDEIGLVITHITDKGFYKVEAVGGVDPQTLIGCRVQFKNRT
                     GVLGVEGETMEDLKNNSKNLSFENLFLDLGFSCRTQAEEFAPIGTFGTYTACFADLGD
                     RIVSKAMDDRVACAVLIESIRNMKDSLNNIIYAFTVQEEVGLVGSSVAGFNYDIDMAV
                     AVDVTAAGDTPKALKRISMELGKGPCIKIKDAASVSDISVVNKLKQAAQANMIPFQFE
                     VLLFGGTNAASYQRTKSGIPSGTISITTRYVHSPNEMVSMDDVKNTVKLVAALSNTEI
                     "
     gene            complement(16492..17520)
                     /locus_tag="PLU28_01165"
     CDS             complement(16492..17520)
                     /locus_tag="PLU28_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOG33705.1"
                     /translation="MQKYLKELVEISGISGKEEKVMQFIKKNIQDKVDSVSVDNVGNL
                     IAFKKGNDSSKKIMFAAHMDEVGLMVTKIYDDGTLGVAQVGGVDPRVLKAQRLKINDE
                     IVAILNSTPIHLDHNLTAVTGYDSIKAYAGFKNKDEAKLKVKIGDMVTFDVKYYENNE
                     YAVCKAFDDRCGCSAMMDLIDFFDSESKKPFYDTYFAFVVQEETGLRGSGVAGFSIQP
                     DVAVILEGTTAGDNPELEKTSWATHLGDGAVLTFMHSGVVLDKRIFEYLVETSKKNNI
                     KHQFKMRTAGGTDAARLTRTVDGIPCAVISTPCRYIHSANSIINKNDYNSVVELIKII
                     SVEGRILK"
     gene            complement(17523..18539)
                     /locus_tag="PLU28_01170"
     CDS             complement(17523..18539)
                     /locus_tag="PLU28_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOG33706.1"
                     /translation="MKTIELLKELTDSFGVSGHEEETFKVIEKILRENYQNLTYERKG
                     TGSLLVKMGNGSKKVAFFAHTDEIGLVISKITDSNYARVAGVGGVDPRTLLAKRVIFR
                     TASGNKLGIIGMLAPHLQAPSTKDKSPSFDQIFVDFSVSGGTKDLQVGDMGTLEVFAA
                     EMQGDKLTSKALDNRAGVAALMRSIDYLSDLKFDGQLVLCFNKGEEIGLIGAKGMSYE
                     VNPDYAIVVDVTFASENNPNFETYKLGDGPVVAVGAPITPSVFEQISSLAQENNIQYQ
                     IEAVPGSSGTEADVVQLARNGIKTGLISIPLLNMHSPVETVSIQDIDNTARLLAIFAS
                     KAGR"
     gene            complement(18551..19153)
                     /locus_tag="PLU28_01175"
     CDS             complement(18551..19153)
                     /locus_tag="PLU28_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HOG33707.1"
                     /translation="MEDIIYAKIIDGDMVFFFQKGYTQKELLESFEKKLNTMKSFFNI
                     GDSIYIFLEDESQYNMIPKIAKLAVSQSIKLAGSFFGELPSMKKNKKELELTNTKIYR
                     KHVRSGQAIENPGDIIIFGNINSGAEVKAGGSVIVFGKVSGILRAGLNTKKNVFIVVS
                     EMASPLVEIAEVPFYNHDWPKGPISIRIEGTKAIVEPLEI"
     gene            19406..>20449
                     /locus_tag="PLU28_01180"
     CDS             19406..>20449
                     /locus_tag="PLU28_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOG33708.1"
                     /translation="MDQENKINELIEKKALWYEKTFKKTVSRFPERKKEFKTSYGKVV
                     DPVYTPADVKDIDYAKDIGFPGDYPYTRGVQPTMYRGRFWTMRQYAGFASAEESNKRY
                     RYLLEQGQTGLSVAFDLPTQIGYDSDDEMSEGEVGKVGVAIDSLKDMEILFDSIPLDK
                     VSVSMTINSTSAILLSMLMAVGEKQGVRHDQLRGTIQNDILKEYIARGTYVFPPDPSM
                     RVIVDIFEYGSKNLPKFNLISISGYHIREAGANAVQEVAFTIADGIAYVEAAIKAGLD
                     PNVFGTNLSFFFNAHNNFIEEISKFRAARKIWAKVMKERFHVTNEEALKLKFHTQTAG
                     STLTAQQPMNNIIR"
BASE COUNT         6720 a         3360 c         3552 g         6817 t
ORIGIN      
        1 ttatatcaaa ggctactact ccaaacttga tcttacaatc tgaaaacctt tttttaccgt
       61 atatctgttt taaaatatca tatactagat cattctgggc tatccctctg gaagaaagaa
      121 gccttgtaaa ggagttaact tttgatgata aagcgttgct tagatcaata aacttctggg
      181 aatttttttt gattatatcc tcaaaatctt tcaggaactc aatttttgaa ggatttaaag
      241 cataaacgcc tccgacaact gatccggcac tacagcctgt gacaatgtcg aattttatgt
      301 tcctttttct cacaatctca ctgatgaaag caatatgtgc aattcctttt actcctccgc
      361 ttccaagtgc aagagcgttt atcattgctt cctccatttt ttacagagaa ggactcagta
      421 aaaagtaggt tatcagagct cctaccgcta ctccaagtac tcccgaaatg gttgaggacc
      481 aaaaaatatc ttgattaggc ttttgtagtt ccgatatctg cttttccatc tctgtgattt
      541 ttaatgaaag ctcagaagat gctgtaactt tctgaactga taattcaata tctgctactt
      601 ctttttttga cgctttttct gcattcattt cgttgtattt tgaactaagt gaaagaactg
      661 tttttttggt atcagacata tcggattgca atttaattag ttctgatgca aggtcagaag
      721 tgagctcacg tttgatttct gttttcatat tgcttaaatc aactgtttcg gcaatcttag
      781 tagtctcagg agatacagtt tgatttttaa gcagtatata agattttact aaaaaatcat
      841 aatcttcttt taatttctgt atttcttttt tattaatttc cgaaacaaga gagttactct
      901 gtttcaaaag atcagaatta tatatgagtt ccaatttatt ctgattttca taaacaagag
      961 aagaaagatc ctctaacttt ttttcatttt ctccgactct tttacctata gaggcaacat
     1021 ttccaatagt attcaaggta tctttatcaa gttttataag tgatgaaagc gagtttatct
     1081 gagattcaac agaatctgtt ctcaatgaaa gcttctgtat actttcattt tgtgcactct
     1141 gggttttttc tgaaattttg aacttttcgt catactcatt ggctttaata gtatatatat
     1201 ataactgaga gagcttatca gagtacatgt taagatattc ctgcataagt cttacttcct
     1261 gctgcaagtt gacaaaatca tttttaagga cataatcagt tatacttgaa ggtatagatt
     1321 ggtctgacgt tgcttttata ttttcaagca tctgacggag ttctccaaca aaagaattaa
     1381 catcggaatt tttgctttcc aactcagaaa gtttttttgt aaaatactcg tttctgccat
     1441 catatgtgtc tataaatgca tttatgtact cgactctgtt attaaggtta tcataaagag
     1501 ttttggtatc cttaatgtcg ttagcaagag attgagtgtc ttttttaatt ataagcagtt
     1561 ccttatcatt ttgtacagta ctttgaaacg ccggaactgt ttcttttgat tccagataga
     1621 caagtcgtga gataatatcg ttaaaacgtt tttcatattg attctgtccg gagtactcaa
     1681 gagcagctat acgatctctc aaagtgctca aagttgcttc tgccgtctga ttgtaaaatc
     1741 ctatgtgact gttgacaata tttcttaaag ctactttcat tccttccatc tcactgtcaa
     1801 gcctttgttt ggcataatcg ttaagttgct gacgattttt ttcttcatat tgagcaaaaa
     1861 gaaaacttat agaatcagtt gttttgtcaa gcttttcttc tattcttaat agttctgatt
     1921 gaaagagatc gaccttttta tttgactgtt ctgaaaaaga cataagagtt ttaaggtagg
     1981 aatcggctgt ttttttcgct gtgtcatttg caatagccac ggcattgtcc gaaattactt
     2041 taaatattgc actatccgta tcaagcggtc catctatgtt cataaatttt tctagtgcct
     2101 ctattcttga tttctgctgt gctatgtcac tgtttaatcc ggaaattttt gaatcgattc
     2161 ttttctccat agaaattatt ttactatcat agttgtaaag agttgtctct atattgttta
     2221 tacggttttc gagactttca gtatcaatct gggatattct tttttcaaaa tcatcgaagc
     2281 tgctagagta ttttttatca aggtaatcta tgagatttgc tccgaaaaag gcaatatcaa
     2341 atctcatgac gtttgacgaa ccattgaatc tgccctggct gtccagttca agaagaccat
     2401 cagttaccat acgcacaact tgtgaatagt atggagaaga aggtttaagg tcgcgtatct
     2461 ctaatgaaaa tgtcagaata gagacaaaaa caaaaataat tacaaggaaa aattttctca
     2521 tctttatacc tccgtttact ttaaaacagc aagatcgtca atgaaaaggg actgagcagc
     2581 gtcaatatat attacaaact ttataccttt ggtttctatt acatgaggtc cttttgaaac
     2641 gtatactata aactgtgacg gtggataaat tactccgtca attttatact ctcctgaaga
     2701 ataaggcctt atgggaattt tatcttcaaa tataaagtta agatttttaa cttcctggtc
     2761 agctatatct attttttcag ttttttcgat attctgatat tttactgtca cagtatgccg
     2821 tcctgtagac aaaggatagc caaaaacgcc aaagtatcca ataagttggt tgtcaatata
     2881 tataagagta ccgtcgacat tgtttattat atttacaaaa ccatatccag gtgaatcgat
     2941 tataagaacg ttagtttttc cagactctgc agaaatattg tatttttttg taagactttc
     3001 aaactgaaaa gtctgtgttt tagagataat atttgcaata aattgtgcag gtacggtact
     3061 tatctcattt tgataagaga tactcttttt ttctgaagag taaagattca taaccgttat
     3121 tttgtttata tcaatgatct tcggagcttt ctggtcaagc tttataccaa gcagatccga
     3181 agtatttttg ctgtatatct gaagaaaatg ttccttttta tcggcaacta taaaagaaat
     3241 gcctgaacca aggtttattc cgtcaagaaa cacttcagca ttttctgcgt taagaagttt
     3301 gaaagtcctt atatcttctt ccaaacttac agtataactt atctgaggag tggcctcact
     3361 tatatttgca gaaagcttct taggagtata aagagctttt gataccgtaa atagatgatt
     3421 tcccatagga atctgtgcgg taaacggagt tattccaaga tactcattat tcaatgtaac
     3481 taaagctcct gaaggctctg aagaaaaaag cacagcgcaa gctttggtca ttgtaaatat
     3541 ataatcgttt gttttagagt cagttttaaa actgaaatcc atatatccag gtgcttttag
     3601 tataatatcc tttccctcca gcaaagttaa agccgtcgca taaggagtag tgcccagatt
     3661 ttttgagcct acaaaaatgc ttgcatcaga aggtatggaa gatatatatg caaagtcagt
     3721 tcttttaagt gtaacagaca gcttgttttc ataaaaaggt tttaagtcaa ttgtaactgt
     3781 ttgggaaaga taccctgctt gctctatctt caattctctt attgtagagg gcaggtaagt
     3841 ttgggtatcg ttaataactt tctggcgtat attattattg ttgaacatat aatttatata
     3901 gaaactgtca ctattcgttt taatgttgag aatacttagc gggacctgta aagctgaaat
     3961 ctctaacttt gaacctttgg taccggaaat atccaatgaa taatctgaat atccaggttt
     4021 tttaacttcc agcttatggg ttgtatctga cagatctatg ttgtatacgg acttttcaat
     4081 gattttttta aaaattccgt ctataaatac tgaacttcct tctaaaccag aaagagtaag
     4141 cgtaaatgaa aaagataata tgtatgccaa aagaataaaa accgttaaaa agagtctttt
     4201 accgatattc cccactctta accacctctt tgattgtttt ttgtagctca cttaattttt
     4261 ctataagttc tttttcattt cccaacctat cgataactga ttgcataact tctactcttt
     4321 ttttgattac tttggtaata agttctcttc cagaatcggt aagtttgatc ctatatactc
     4381 ttttgtcttc ctctgactgc tccctgtcca aaaaataaag tatttccagt ctttttatta
     4441 gaccagttgt agtgcttttg gttactccga gatttttact tatttcgctt aactttttct
     4501 ctccgtcgaa gtataatagt tgtaatacat caaattgagc aggagaaatg tcgtaatctt
     4561 tcagaacttt ccttccagct gccctgatgt ggaaacagat cgaccttaaa atgctttcca
     4621 tattttctgc gttcttgtac attttgatac ctccccgagt tttaaaatta ttattatcac
     4681 aaattacaag aatttgtagt aaaataatta caatatacag ctattctatc ataaaattct
     4741 ggaggaacaa agaaatgagt aaaaatataa ccattacagc tatgctttct gtgctaacta
     4801 taatactttt ttcatgtcag atgtttattc ctgtggtagg aatcttcgtt gcatattttt
     4861 ctattattcc acttatgttc atatatgaaa tgactaatgc taaatatttt tttatgtcgc
     4921 ttattacatg catatctatg gttatgattc tgaatgatcc ttttggatgg attttctttg
     4981 atctgcttct ggttccgccc gcaattacaa ctgtaacaaa aaacaaaatt cttaagttga
     5041 taatgattgt tcctatagag ataggttttc ttcttttatt taagatactt gtagtaaatg
     5101 gttcaaaaat tgatatgatt ttatcaaata tatggtatat acttggtata ctgttttatt
     5161 tcgcaggaac tttcttttat cgtaaagctt ttttgatatc aaagatgcta ctgaaaaaaa
     5221 taaactacta atttaaccaa ttgacgtaag ttatgataga atataaatta tatatttatt
     5281 taggaggaac agtgatgagt atactaaaga ctatccagaa gattatagaa aaagttcttg
     5341 ctgatatggg aatttcacag agtctggatt ttaaagttga agtgcctcca caggatgatt
     5401 taggagattt ttcaactaac gcagcatttt tgtgctcaaa aatattgaga aaatctccaa
     5461 aggtagttgc agatgagata tctgaaaagc ttagacagct tgacacattt gtgagggttg
     5521 acaatataaa cggttttata aatatgtttt tggatcatag tgtataccag atggcatgtg
     5581 ctggtataat aagtaatccc aggaatatcg gtaaaagcaa tattggaaat ggtgaaaaca
     5641 ttcaatttga atttgtaagt cctaatccaa ccggaccact tacaattgcc catggaagac
     5701 aggccgttat aggagatgtt ctatgtaaca tatttgatga aagcggctac aatgtacaga
     5761 gagaactcta tataaatgat gcaggacaac agatagagta cctttctaaa tcagtttgga
     5821 taaggtataa tgaacttttt ggcattaagc atgagcttcc tgaggatggc tatcatggcg
     5881 aatatattat tgatgttgca aaggaattta agaacgaata tggagataag tataaagata
     5941 tatggaatga ggaagtaaat gattttttca aggaatactc acttaaaaaa atgctttcta
     6001 tgataaaaca aactctaaaa agacttggag tgaattttga tgtctacttc agcgaaaagc
     6061 ttctcataaa ggaaaaaata gtaagcagaa tattggatac tttggaaaaa tctgatagca
     6121 cttatgcgaa agacgatgcg gtatggttta aagtatcaaa gtatattgat gaaaacgata
     6181 aagttctcat aagatcgact gataatcgtg cgacctactt ttgtgatgat atagcttatc
     6241 attactataa gtatcataga ggtttcacta aggtatacga tatctgggga gcagatcacc
     6301 aaggtcatat tcctcgcatg aaagcttcaa tgaaggcttt gggaatacca gatgattttt
     6361 tcaacgttat cttgcatcag tttgttacac taaaaaaagg cgatgaggtt gtcagaatgt
     6421 caaaaagagc cggtaatttt gttactttgg atgaccttat agaagaagta ggaactgatg
     6481 ccacaagata tttttttgct atgattgatc cagatactcc tatgaatttt gaccttgagc
     6541 tagctgtaaa aaaatcttca gataacccag ttttttatgt gcagtatgct catgcaagga
     6601 taagcagtat ccttaaacag gcagaagaac gtaaaatcga ttttaagatg ttcgaaggat
     6661 taagtgaggt aaaagagaaa gaagagcttt tattgataaa agaactttct ctcttttcaa
     6721 atgtggttgc agaagcagca aagaacagaa aaacaaactt tattacaaca tatcttgaaa
     6781 aagtatctag tagatttcat actttttata ctaagcattc tattttaaat acagataatt
     6841 cacagttgat acaggctaga ctcaacatct gccttgcaac caaaataata cttgagaggg
     6901 gactggcttt gcttggagtc agtgctcccg ataaaatgta aggggggtca tccatgtacg
     6961 aaatacagag aaatcctact gaaaaactta tcattcagaa aaacgagttc aaaggaaaag
     7021 agtatattga catccggatt ttttatgaag atgaaggtga gtacaagcca acaaaaaaag
     7081 gagtcacatt taatccggat cagattgatg aagttataaa agcccttgaa agtataaaag
     7141 aagaatcaca gaaataaagt atctttagaa aattgcttca atacaaataa gaagatattt
     7201 aatattttta taagtcataa gttcagagcc aaatacattg gttctgttct tgtgtaatat
     7261 tgtcaaattt tttttaagtt ataaagagtt gacgtaatag tggcaaagtt taaatttagg
     7321 agataccaat gctggataac aaaggttttg atttatgggc taatggatat gacaaagaag
     7381 taggcatttc tgatgaagaa aacagatacc cttttgccgg atataagagt gttctgtcaa
     7441 agatttatca taaagtacta gaaaagaata agccaagtgt tcttgatatc ggttttggaa
     7501 caggtacact tactgctaaa ctttatgagc agggatgtaa aatatatggg caggatttct
     7561 ctgtaaagat gcttgagatt gccagcaaaa agatggcgaa ggctaagttt tatcaggggg
     7621 atttctctct tggtctggta aaaccgctga gaaagtacac atatgatttt ataatcgcaa
     7681 cctactcact tcatcatctt gctgataaag aaaaaatagt ttttcttcgg gaattgaaag
     7741 aattactgaa cggagacgga gtgattcttg tcggcgatat agcatttgaa aataggattg
     7801 aattggaaaa ttgctgtaat gaagcaggct cagaatggga tgatgatgaa gtatactttg
     7861 tagtggatga attgaagaaa gagctgccag aattgatctt tgaaaaggct tcatactgcg
     7921 ctggagtttt atactttggg caaaatactc ttttttaaat aagttgtact taaaaaaaca
     7981 ataattctga ttttttacat ttcataaacg tatacagctt taaaaataaa atgccctata
     8041 gggcatttta aaattttagg gtactaagta tgagagaaat actctgtgca ttttaggttc
     8101 gatcttatac taaatatgtt gaatttggaa taaaatgagt taaagtgcat tttctgaaga
     8161 aaaattttta agattttcag cattttctat ttcgtcaaac tttttaattt cttcagcatt
     8221 tttaagatag aaactcattg cctgattaag aacactagtt attgtagtat gatttctggc
     8281 tgcaaactcc cgtatctgct cataaagatt tgcatctaga tagagattta cctgcttgac
     8341 gtctcgcata tcctgtcaat cctccttcat catataatat acttttgatc tatgataaga
     8401 cttacgtgaa tataaattaa ttctatctta tgtatcttaa taaaatgtaa attttaagta
     8461 aacataagtt tgacaaaaaa caaccttagg aatagcaaag tatctataaa ctttttatat
     8521 gcgaacgttt ctgatataat tttagtgcag atgaagaaaa atgaaagaaa tggaggaaga
     8581 aaaatgaaaa aggtatattt atgtatgtta ttggtttttt tggttgtaag tggtttttct
     8641 gattatattg caatcggtga agacggaaag atcgttcaga taacacaaga gaatcagtct
     8701 atacctttag gcttaaaagt ggttgataaa gagtttaacc ggaagttctt ggatcttttg
     8761 aaaggagctg aaacggaagt tttatatgat gctcctatac agatagtttc atatcagttt
     8821 gaaagaatta tggtggattc ttccataaaa aagagatttt cattaagcat aaagaatact
     8881 tcgtataaag ctgtgacttc attttcggta aaaattttgg cttacgatat atataattat
     8941 gaacttccga tagattataa gcaagatagt actgttacgg ttgaaagaat tattctgaat
     9001 cctggagata tatacgataa ccttaataca atgagttttt attttcatga ttcaatatat
     9061 gcggtagctc cctttgttat tagtgtcgta tatgccgatg gaagtacttg gcagggcggt
     9121 ataaggtagt tcaaggaaag acggttaacc gtctttcctt gaactaaata gcagttttag
     9181 gagaataaag gtaatgattg tagaaaatgc ccccataaaa ataaacgata tttttaagga
     9241 aaatttatcg cctataactc cgaaggcagg aaaaagaacc atcatataga gactgaaaac
     9301 cattgagcaa aaagaaagta tggtagctct attttccgag ggaattcttt tattgatgta
     9361 gttgctggaa acaacataaa gaactccatc tgcgatattt gtaaggataa aaaaaacata
     9421 aggtactgtt gaaaaagcta ttgcccatat gctgaaacaa ctcattatac ttagtacggc
     9481 aaaaagtttt ttttcatcaa agattttttc aagtttgtga gcgaaaaaag cacttgttgc
     9541 acttgcaata gatgataatg ccataaaaaa acctatactt aaaggatcat agccgataga
     9601 aagaaagtag ttctgcagat aaaaaaataa agtggttgta aacattaaaa atgcttcaac
     9661 gaaaatgatt acaaataaaa gttttttatc tttttttact atacggaaac tttcaaaggc
     9721 ttgtttaaaa acatctttaa tatttttgct tccagaatca cttttgatta aaggttcctt
     9781 aaaacttagt gattgaagga tagagatgac tcctatcatt atagcgccgg tatatgcata
     9841 gatgtaatta aaatttgaaa gatatccgcc caaaatatat gcaccaaaca tcgctccttg
     9901 ggtaataact tcattgtacc ctgatatctt agtaaaatac ttttcatttc cattttcttt
     9961 aagggaatcg taaacaagtg cttcacctgc tcctgattcc atagtataag aaagagcaca
    10021 tattcccatt gcacatgcat acataaaaaa actgtcggaa aaaagtatta ttaaaaagta
    10081 tagtactgag aatactctgc ttagagtccg gctgactttc cttccaaaaa tatctgcaat
    10141 tgcccctgtc ggcacttcca tcgcaaaaga agtgatatga aatatactct caagcatgcc
    10201 aagttctata agagtcattc cttttgatgc aagataaatc atccatatgc tgcttgtaag
    10261 attaaagttg tacaaaaatg tgaataaata atttctctga atattttttt ttaactctgc
    10321 cgcatagttc atttttttca actccttatt ttttttaact tccgtcgaaa aaatgaactt
    10381 ttgacccaaa atatctaagc gaaaaaattg ttttcatacg tgtacctcct gttttttggg
    10441 tatagtcccc caaggtaagt atattatacc attgtacaca taaggagtga tagatatata
    10501 taaggtattt attgttgaag ataatttaaa ggctcttaag gagcttgaga ttcttttgaa
    10561 acgttatggg ttcgagattg tctgtgcaaa ggatttttcg gacattccac tgcagattca
    10621 gaatgtaaaa cctcatctta ttcttttaga tataaatctt ccgcaatatg acggattttt
    10681 tatatgccag aacatacgtg cagtttcaaa agttcccata attattgtga ccgcaaggga
    10741 cagcgatgtt gatactataa tggggatacg acttggagcg gacgactaca taaaaaagcc
    10801 atattcaccg gaaatacttg ttgttaaggt tcaggcagtt ctcagaagag tctatgaaat
    10861 ggcatctgct gatgagtcat gtatgaagta caagggtctt ttttttgatc tgaaggatta
    10921 ctctttgaag tatgaggata aaaccgtaga acttacaaaa aatgaagtta aaatactgtt
    10981 tttactcatg aataaagccg gaagcatagt tactagagat gaacttatat atgagctttg
    11041 gaacaacgat gagtttatag atgataatac tcttacagta aatattacca gattaaggaa
    11101 aaaatttgaa agcttggatt cgtgtgtcat aatagagacc aagaaaggag tcggatatat
    11161 attaaaatga agttcagaga gtatataaag gagtatgaac tatttatttt ttttgaactg
    11221 ctcggattaa taataattga tatctatcta tttcttataa atacattttt tcagcttatt
    11281 atcataatag atattattta tcttttatcg cctcttcttt ttatgatata taactttcat
    11341 aaaaagaaag agtattattc tacgatctac cgtaccatgg ataatcttga gaaaaagtac
    11401 tttatcagtg agtttatggc acaagagtcc tttatcagtc ttgaaaatga aataatgaaa
    11461 gatattctta acacgatgtt cctttcaatg tacgaagata taaaaaattc caaagaaaaa
    11521 caattagact atcaggaatt tcttgaaata tggatacatg aagtaaaaaa tcctttactt
    11581 acacttaaac ttattgttgc aaacaataaa accaaagtaa acaaaagtat cctggaagag
    11641 ttggaaaaga ttgaaaatct tttggaacag atcctttact attcacgata cgaaaacctt
    11701 gaaaatgact atgtaataaa agagactctc atagaagatt ttgtcaaaaa tgccataaaa
    11761 aaacattcca gacagataat agaaaataat ataaaaatac aactctcaaa tctcgataat
    11821 atagttaaaa ctgattcaaa atggatggag ttcgtatgta gccagattat ttcaaacagt
    11881 ataaaatatg tcaaggataa tccggaggta aagatattcg cttttgaaga taataactct
    11941 gttgcacttg tttttgaaga taacggtata ggaatcaaat ctaaggatct cagccggttg
    12001 ttctcaaaaa actttaccgg ttataacgga agactggaaa agggttctac cggatttgga
    12061 ttatatatca taaacagaat agtacataag cttggccatg aaatcacagt cgattctcag
    12121 gaaggcagtt atacaaaaat agaagtgaaa ttttctaaag cagttcacaa atggtgactg
    12181 cttttttatt ataaaatcta aggaatttat cttacaaaac agtaagaaag gtgaaagatt
    12241 ggaaaattaa cttttccaat gtaacatact aaaatatttc taggaggtgg atgaatatga
    12301 aaaacattct taaaatagaa aagctagaaa agatttatgg gttgaggggg aacgtctaca
    12361 aagctcttga tggagtaaac tttgatgtat atgaaggaga gtttcttggg ataatgggac
    12421 ccagcggagc tggcaaatct actcttctga atatcatatc gactattgat aaacccacaa
    12481 gcggtagcgt agtaattaac ggacaaaata ttgtcaatat gaaaaagaat gaactctcta
    12541 aattcagaag agataagtta ggatttgtct ttcaagagta caatcttctg gatacactta
    12601 ctgtaaaaga aaatattgcg ttacctcttt cattagccaa tgtctcttac agagaaataa
    12661 accgcagggt taaagaggta tctaataatc ttgaaattgt agacatactt gataagtttc
    12721 cttatgagat ttccggaggt caaaggcaga gagcggctgc agctagggca attattgcag
    12781 accctgcact tattcttgct gatgagccta caggtgctct tgactcccgt tcatcagaaa
    12841 ctcttttaag cacactcaaa aacataaacg aaaacatgaa cgttactgtg cttatggtaa
    12901 ctcatgaccc atttgctgca agttactgca aaagaattct ttttatcaaa gatggaagaa
    12961 tattcaatga aatagtaaaa ggcgaagatg aaagaaaaaa attttttaca agaattcttg
    13021 atgtactggc agttctggaa ggagacatag gaaatgttat ttaagcttat aaaaagaaat
    13081 ataagaagaa gtttcagaga ttatgtaata tattttggca ctctgatact atcaattgca
    13141 attttttatg tgttcaactc tcttgatagt cagcccgcat ggtcgggaga tgagtatcct
    13201 caggctaaaa ttgttatttc atctttaatg ggaggtcttt cgtttataat atcttttatt
    13261 gttggatttc ttattcttta tgccaacaac tacattataa aaagaagaaa aaaagagata
    13321 ggtctgtata tgcttttagg aatgagccaa aaaaaagttt cattcatgtt gctttcagag
    13381 acaattataa ttggatttct ttcatacgta gttggtataa ttataggcat atttgtttca
    13441 cagatttttt cgctaataac gatgaaattt tttgatatag caataaataa aattgaattt
    13501 gtgctttcat atcctgcagt aataaaaact ctgatctttt atggtattac attttctctt
    13561 gtagctgttt ttggatcatt tggagtatca agatttaaac tcatagatct acttatggga
    13621 gaaagaaaaa acgaagagtt taagatacgc aagaaggcag tggttgcaat tatttttgtt
    13681 ttgtcgtgca tagatctttt tcttacatat tattttgccg actatgtcgc aagcaggctt
    13741 ttggtaagtc ctcagtcaat atttgcaata atatttggtg gatcactcgg aacatacgga
    13801 cttttttatt ccggtttttc aatgatattt ggagtaataa aaagtatgat aaagttttat
    13861 ttttcaaaaa taaatgcttt tctttcaaga caacttttga acaggtttaa tacggatatg
    13921 ggaatgcttg caacgctgac ggttcttttt acattaacgc taagtgtttt tatatcggga
    13981 ctaggattga aaaattatat agaaagcagt gtagagagca attctccatt ttcattcaat
    14041 atcactgtat atgaaaatgc tgaaaaaata aagcaggagt ttgatgaagc tgcttacaga
    14101 ctaggcaaag actatcttaa agattcatta atggttagat cattcaggag tgatataagg
    14161 attaaaagta taagcccgga caaagagtac tttggaaata gcgaactttt acgtaactat
    14221 ttaaatgata atttttatat tatgcttcag agtgatttta acaagcttat taaagcgaag
    14281 aatataggag aagaagagct tacaggcaac gaagcactat tatatgtacc tgatggtagc
    14341 aaggatttta aagagtatct tgaaaatgaa ttatctttta acagaaaact ttttcttgga
    14401 gatagtcaga ttgagatcaa aaaaatattt ccgcaacata aaataggtaa ttttatggtc
    14461 tttggaagct gtgcactgat tataccggac aaatttgatt atattaccca gggatataaa
    14521 actatatgga atattgattt taattttaag actggctatc cttccgaaat agataaagag
    14581 tttcaaaaaa tatcagatca gtatgagtta aggatgaata ctctcaaaga agcaaaagct
    14641 aacgcttcaa tgtcaagagt tcttgtgata tttgtaattc tttatttagg cataactctg
    14701 cttatcgcaa gtgctgctgt tatggcactt aaagaactaa ccggagctat agacagcaaa
    14761 aaaaactatc agatgctttc caaactagga gcagacgaca ggcttataaa aggaaccata
    14821 aagaaacata tgagatttta tttttttatt ccgcttcttg tggcattctt tcataatatt
    14881 tttgcagtaa actttattac taagtttcta aatgctttta atgctcagaa cacttttttg
    14941 attactctta ttgctatggg agtctttctg ggggtgtacc tgatttatta ctacataact
    15001 agcaggatgg ccatacgtat aatagttaaa gaggaataaa atgtcaaaat atttttctaa
    15061 aaaaatacgg tgctgaaaag caccgtatta agcttactga ttaagttttt tctgaatctc
    15121 aagaactata gcctgcgaag tagcaagatt tgttgcaaga ggaatattgt gaacatcaca
    15181 tacacggagc agtgccgaga catcgggctc atggggttgg gcagtaagcg gatcacgtaa
    15241 aaaaataaca aaatcaattt cacccgaaac aatcatagct cctatctgta gatctccacc
    15301 gtaaggacct gactcaaaag ttcttatcgt ctcaagacct attttttctt taagaattga
    15361 tccggtggtt ttagtgcaga aaagttcaca tttacaaaat acatccttcc attcacgtac
    15421 aaacatagcc aaatcaattt tctttttgtc atgggcaata agagcgactt ttatcattga
    15481 atatcaccta tatttctgtg tttgaaagag ccgcaacaag ttttactgta tttttaacat
    15541 catccatgct gaccatttcg ttgggagaat ggacatatct tgttgttatt gagattgtac
    15601 ctgaaggaat accggactta gtacgttgat aactggcagc attcgttcct ccaaaaagaa
    15661 gaacttcaaa ctggaaagga atcatgtttg cctgagcggc ctgcttaagt ttatttacta
    15721 cactaatatc acttacactg gcagcatctt tgatctttat gcaaggtccc tttcctagtt
    15781 ccatacttat acgcttaagt gcttttggag tatctcctgc agcagtaacg tctacagcaa
    15841 cagccatatc aatatcataa ttgaaaccgg caacgctcga acctactaat ccaacctctt
    15901 cctgtaccgt aaatgcataa ataatgttat tcagactatc tttcatgttt ctgatgcttt
    15961 ctataagtac ggcacatgca accctgtcat ccatggcttt ggagacaatc ctatctccaa
    16021 ggtctgcaaa gcaagccgta tatgtaccaa acgttcctat aggagcaaac tcctcagctt
    16081 gtgttctaca ggaaaagccc agatcaagaa aaagattttc aaatgaaaga ttttttgagt
    16141 tgtttttaag atcttccata gtttcacctt caactccaag cactccagtt ctgtttttaa
    16201 actgtactct gcatccgata agagtttgag gatcaacacc tccaactgct tcaactttat
    16261 aaaatccttt gtcagtaata tgagttatta caagacctat ttcatccata tgagcatcga
    16321 aaagaatagt ttttcccgat cttccttttt taaaagcaat taaatttcca attttatcaa
    16381 tcttataatc atcaacataa tcttttatct gtgaaataat aaagttcctt acctcatctt
    16441 cccgtccact tggagaatat atttctgtta tatttttaat tagctctttc attattttaa
    16501 aatcctccct tcaaccgata ttatttttat aagttcaaca actgaattat agtcattttt
    16561 gtttattatg ctgtttgctg aatgaatata acggcaagga gtcgatatta cggcacaagg
    16621 aataccatca actgttcttg taagtcttgc cgcatctgta cctccagcgg ttctcatttt
    16681 aaactgatgc tttatattgt tctttttcga tgtctctaca agatattcaa atattctttt
    16741 gtcaagaaca actccactgt gcatgaatgt aagaactgct ccgtcaccca gatgagttgc
    16801 ccagctggtt ttttcaagtt ccggattatc cccggctgtt gtgccttcta aaataaccgc
    16861 aacatccggc tgtatagaaa aaccagctac gccgctgcct ctaagaccag tttcttcttg
    16921 tacaacaaaa gcaaagtagg tatcataaaa tggttttttc gattccgagt caaaaaaatc
    16981 aatcagatcc atcatggcgc tacagccaca cctatcgtca aaagctttgc atacggcata
    17041 ctcattattt tcatagtatt taacgtcaaa agtgaccata tcaccgattt ttacttttaa
    17101 tttagcctca tctttatttt taaatccggc gtaggcctta attgaatcgt atccggtcac
    17161 agccgtaaga ttgtggtcaa gatgaatagg agttgagtta agtattgcaa ctatctcgtc
    17221 atttatttta agcctctgag cttttaacac ccttggatca actccaccta cctgtgccac
    17281 acctaaagtt ccgtcatcgt atatcttagt taccataaga ccaacttcat ccatatgcgc
    17341 tgcaaacatt atttttttgg aagaatcatt gcccttttta aatgcaataa gatttccaac
    17401 attatctaca gaaacggaat ctactttgtc ttgtatattt tttttgatga actgcattac
    17461 cttttcttct tttccagata ttccagaaat ttcaacaagt tccttaagat atttttgcat
    17521 tcttatctcc ccgcttttga agcaaagata gccaataatc ttgctgtatt atctatgtct
    17581 tgtatagaaa cagtttcaac aggtgaatgc atatttaaaa gaggtataga aatcaatcct
    17641 gttttaatgc cgttccgcgc aagttgaact acatctgctt cagtaccact tgaaccgggt
    17701 acagcttcta tctggtactg tatgttattt tcctgagcca atgaacttat ctgttcaaaa
    17761 acggatgggg ttatgggagc gccgacggct actaccggac catcacccag tttatatgtt
    17821 tcaaaattag ggttgttttc tgatgcaaaa gttacatcga ccacaatggc atagtcagga
    17881 tttacttcat aactcatgcc tttagcacct ataagcccta tttcttcacc tttattgaag
    17941 catagtacaa gctgaccatc aaatttaagg tcgctcagat aatcaatgct tctcattaat
    18001 gcagctacac cagccctgtt atcaagagct tttgatgtaa gcttatctcc ctgcatctca
    18061 gcagcaaaaa cttccaaagt tcccatatct ccaacttgaa ggtctttggt tcctcccgat
    18121 acagaaaaat caacaaaaat ctgatcaaaa gaaggagatt tgtcctttgt ggaaggagct
    18181 tgcagatgtg gggcaagcat accgattata ccaagtttgt ttccagaagc tgttctgaaa
    18241 ataactcttt tggcaagaag agttctgggg tcaactccgc caacacctgc tacccgtgca
    18301 taatttgagt ctgttatttt gcttataaca agtccaatct catcagtatg ggcaaaaaaa
    18361 gcaactttct tacttccatt tcccattttt actaaaagac ttcctgtacc ttttctttca
    18421 taagtaaggt tttggtaatt ttctcggagt atcttttcta tgactttgaa ggtttcttct
    18481 tcatgtcctg atacaccgaa ggaatctgtg agttctttta atagctcgat agttttcatt
    18541 aataaccccc ttatatctct aaaggttcca caattgcttt tgttccttca atcctaatgg
    18601 aaattggacc ttttggccag tcatggttat aaaaaggcac ttctgcaatt tcaacaagcg
    18661 gagaggccat ttcggaaaca acaataaaga cgtttttttt cgtattaaga ccggctcgga
    18721 gtatacccga tacttttcca aaaacgatta cgcttccacc agcctttact tctgcaccag
    18781 agttaatatt tccaaatata attatatcac ccggattttc aattgcctga ccggagcgga
    18841 catgttttct atagattttt gtatttgtaa gttcaagttc ctttttattt tttttcatag
    18901 aaggaagctc accaaaaaaa gagccggcaa gtttaatact ctgtgaaaca gcaagttttg
    18961 ctattttagg tatcatattg tactgacttt catcttcaag aaaaatatat atactgtcgc
    19021 cgatattgaa aaaactcttc atggtgttga gttttttttc gaagctctca agcaattcct
    19081 tttgagtata gcctttctga aagaaaaaaa ccatatctcc atctattatc tttgcataaa
    19141 tgatatcttc cataaatgcc ccccgttaac tattgtatgt gaaacgaaca gtacacaatc
    19201 tcaatatatc ataccacaaa aagtaactat aagaaaaatg catttattat ctttatcggt
    19261 aaacttcaga ttaattagtt tctttgggat ttaatgaagt ctttttcact atatagtcga
    19321 tagtatttaa catccgtatt tgtaatatta caaactttaa tgtaaaataa tactgtaagc
    19381 ttatcttgta ctggaggtgt atctgatgga tcaggaaaat aaaatcaatg aacttattga
    19441 gaaaaaagct ctttggtatg aaaaaacttt caaaaagaca gtgagcagat ttccggagag
    19501 gaaaaaggag tttaaaacat cttatggaaa agtagttgat cctgtttata ctcccgccga
    19561 tgtaaaagat atcgactatg ctaaggatat aggattcccc ggtgattatc catataccag
    19621 aggtgttcag cccacaatgt acagaggaag attctggaca atgagacagt atgcaggatt
    19681 tgctagtgcc gaagaatcaa ataaaagata ccgttatctc ttggaacagg gacagacagg
    19741 gctttcagtc gcatttgatc ttcctaccca gataggatac gattcagatg atgaaatgtc
    19801 agaaggagaa gtaggaaaag ttggagtagc tattgattca ctgaaagata tggaaatact
    19861 ttttgatagt atacctcttg ataaagtgag tgtgtcaatg acgataaact caacctcggc
    19921 catattgctt tcaatgctga tggctgtagg tgaaaagcag ggagtacgtc atgaccagct
    19981 tagaggaacc attcagaacg atattcttaa ggaatatatt gccagaggca cctacgtatt
    20041 tcctccggat ccttcaatga gagttattgt agacatattt gaatatggat ccaaaaatct
    20101 tcccaaattc aatcttataa gcataagcgg atatcatata agagaagcag gagcaaatgc
    20161 tgttcaggaa gtggctttta ccatagcaga cggtatagct tatgttgagg cagcaattaa
    20221 agccggcctt gacccaaatg ttttcggaac caacctttcg tttttcttca atgctcataa
    20281 caacttcata gaggaaattt caaagtttag ggcagccaga aagatatggg caaaagtaat
    20341 gaaagaaaga ttccatgtca ccaacgaaga agcacttaaa cttaagttcc acacacagac
    20401 ggccggttct actcttactg cccaacagcc tatgaataat ataatcaga
//
LOCUS       DAOSHR010000011        19955 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_2193_length_19955_cov_6.157990, whole genome shotgun sequence.
ACCESSION   DAOSHR010000011 DAOSHR010000000
VERSION     DAOSHR010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 19955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19955
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2193_length_19955_cov_6.157990"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            35..601
                     /locus_tag="PLU28_01185"
     CDS             35..601
                     /locus_tag="PLU28_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG33709.1"
                     /translation="MLKKIEAYFMKEIADKAGLIAILSLLLFIPNFYGRVIFLLLLIC
                     LMLPFDIHNKRFSLLISLPFSHREIFLSGFVFVLAVSISTQFIGGALLGVKIAYLMVE
                     CLKSVIFCAFYYGVSMVAVLYGLDNLGIPLLVFFADAIFGAIGPKWSNFYSYISPVHN
                     GNLLFSAVFAAVVFVFSFYLFERKGIQK"
     gene            598..1419
                     /locus_tag="PLU28_01190"
     CDS             598..1419
                     /locus_tag="PLU28_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG33710.1"
                     /translation="MIINIENVNKSFGSKHILNNISFSVQEGEIFALIGPNGAGKTTT
                     IRCIFGELMPESGKVEVFSSPVSSKLKERIAVMTEDRLTFKRFKGADYLKLWKMLYPR
                     WNESIFASFMMHYRFNLEDRVETFSMGMKTLFYLALDMASGADLLILDEPTQNLDPVI
                     RHEVLNMLHDFVLSENKTIVISSHEIYELEEIASSFAIINEGKVLYTDTIDDAKASHR
                     IVEKGERIPHGDVIGLLNEGTLIRTSEDAGRYPNFKEIALAYLQGGKAFNPFERL"
     gene            1423..3900
                     /locus_tag="PLU28_01195"
     CDS             1423..3900
                     /locus_tag="PLU28_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG33711.1"
                     /translation="MISPQNKSLKVLFLFLLVGTVFFGNTVIFTPPKELLTLKTKNFE
                     YIYEEKLYFYVQDIMESADSVYEDYASFYGVRPEGIKVYMLDDVDYVNAFAVGFDNKI
                     RMYVNTADFSSGLLSSNFLSNRMIFCHELNHLFYSNMVSNPFISWIPNQTIKKISNMS
                     SQPSYLHEGLSIYMESKYFGGRFENDLFNMYLKAEVLSPKYPRYELGSGAQVKVWSPA
                     GFNYMYGALFTKEIAGFYSEEVLREIVRLTSSSFFSSISGAFEKVTGDKWSEFLQKIK
                     LKYIEQMSIVGDNGNIQKYEKLGKEYERTGNLRTDGKNLYAYKVMPFSRAGVYKNEEL
                     IIPEVSKFDVSSNGEVIFSKTISNDGNAFNSLYIKCDCPISDTLIAQRVLNFGFADSR
                     TVVYTVNDKGLSGVFVYDLIEKKSSQILGWDKYVMNSVCGTQEDIYLSLTYDGQSDIY
                     RYDRRNASMQRLTDDKEYEADLSYAKGKLFYTKNYNGIFNIFSLDLQDMAVRQMTFSE
                     MGAFSPAVLGDQLYFLSYDWEGYYISKTDPALSTESETLSAKAFMFESTGFVGKNDLS
                     AEKYFENPQHIIVYPYAELSDDILEMDLSKLTLNYGMGVAVISDAYNYMMNYSLVFKK
                     DALKHNLELYFNSLAAVDLKLIYEKNIHSALEVSKDFSFYLKDMYFLKLAATGSADGI
                     TPEKVSVQAQLFQNPYSINKISLYDVALSGSYLYDNTKNSQYFSVGIFKPFYLYNFRL
                     TPFFEYDSLGNTLAGATLYGDLYYPHWNIFDGKRGLNGFSVESTLNYNFKDRKMSCSV
                     YFILDLKTSYWANYSYKVPVFKMPLSN"
     gene            3945..4946
                     /locus_tag="PLU28_01200"
     CDS             3945..4946
                     /locus_tag="PLU28_01200"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOG33712.1"
                     /translation="MKKTFFIIMILGFAVISFTNPIGELFKSALNSNKGIKMTMLNFE
                     SAQMEYEKALIESSNKKALLSAELSMLNERSSYRKGQADFYSEVVDAFFAVKSAQIEL
                     EISQLQLGIVQNDHKNAKLSFEKGMISLNSLKDSEVAVIDAQSQEEKARKDYNDALTA
                     FKRATGTEYADYEVKPCDYKECGFSYEAFKENDLSLKAQQINLQLAEYDLQNLSSNSS
                     YYEKRGLEIKLEISQMNLKNKEESVSESYETALAMVSSLYSSLQSSGVKLQIKQSVYE
                     DSQKRFEKGFVSEKDNINQKISYMSAQKAYNENIKSYIKALVSLIFNCGLSVGEAVL"
     gene            4943..6241
                     /locus_tag="PLU28_01205"
     CDS             4943..6241
                     /locus_tag="PLU28_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG33713.1"
                     /translation="MKKIFIAMLIFIVTVVYGGITEIYNERVEKSTAFMLSENSFNQA
                     VRTLDEKKSFWIPGLTAGFDQIIFGKDGLENKNIVNGHKSIRLNLKANFVKILGASIG
                     VSLPYVYDLTDYSGKFENFNLTVSRPLFTEFYLEVPQAELSVMQSKNAMEKTRWDIFS
                     QLLQDIFNYHNALSTRELINGRTYVTEILYENENDNEKKKTYRQQLTELNKLSINNEL
                     VLNGLNLEYCAELYSQAVQICEKMVLISEDCTQVNFNGTELPSVLLKEDIARRQKEMW
                     FLPYLPNPVISFGIDLLDSLKWHVGLSFEYSFFEKGTVSSKSVESSNRISQYEIAVLQ
                     TANAKESTQHSIEKLKKDLEIYKMEVFASKANMDDSLAELAKNKLLMEKGYLTKEKYD
                     LGSYEHMSKKLDYEKTLQSMYLTCVKLLQEYGLPAGGGTL"
     gene            6238..7305
                     /locus_tag="PLU28_01210"
     CDS             6238..7305
                     /locus_tag="PLU28_01210"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOG33714.1"
                     /translation="MKKRSKVIIWIVVLILIVFVAIKFFRGGSTAADAGQNQLTQNGS
                     VGGYFLTYRVEKKEIPDAVELFGSVDADVYKITSKMNEEIEQVYVKQGDTVKIGDPLV
                     RLSETGLRSKYLAAYRDYESSLTGAPIDVQQKKIDLDLAKKNLDNVIIKSPANGTVKS
                     VSAKVGEYSSQSSELVSIVDTSSLMAKASIDELDIPVIKKGSRVIIEFSSLNVKISGY
                     ISYVSPSAANSGGMVSVPVEISFDEDASKYGVISGMSCKIKVLLMHKQNILVVPVRAV
                     YSDESGEYLLVMDEKGERQKVYIKTGERTVNVVEIIEGIKEGTEIIIEESPDVPVPEN
                     LGKGYYASVCKQGGYDACNRI"
     gene            7286..7984
                     /locus_tag="PLU28_01215"
     CDS             7286..7984
                     /locus_tag="PLU28_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG33715.1"
                     /translation="MPVIEFKEISKVYKMGDVQVNALKSISFSIEHGDYVIIMGPSGS
                     GKSTLLQILGCLDRPSSGEISIDGTQTSKMNEDQLAKVRNRKIGFVFQGFNLLPKLTA
                     LENTMLPMIYGNVYKGKERTERAKELLQDVGLNERINHRPNQLSGGQQQRVAIARALS
                     NNPSIILADEPTGNLDTQSGKEILEIFGRLNSSGKTVIIVTHDPDMAKKGNKLIKLRD
                     GLIEKIEVTGIGNT"
     gene            7971..9197
                     /locus_tag="PLU28_01220"
     CDS             7971..9197
                     /locus_tag="PLU28_01220"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG33716.1"
                     /translation="MEILKETMRSLLTNKMRTFLSMLGIIIGVMAVITVTALGQGTTS
                     RVSEMMNSLGSNVILVEQGYMGGYGGMSLSSVKNALSIQDVDNIRSMAPSVKYVVPVL
                     NPGLELQYEKRSVYSMTIASRPEFFEMMGLETQAGRILDNEDEKNVAKVCVIGSDLAL
                     ELFKQSDPIGKTVFLNQNYGESGRKTSVQVIGVLKKTGSKFFFNADRTVFLPYSTGDM
                     RIKQSNGTVDSIIVSAKDGLSAKACLEINYVLYLRLKNIQKYVITAQESLSQMVGQVT
                     GVINLVLMAIAGISLLVGGIGIMNIMLVSVTERTREIGIKKAIGASRKRILLEFLVES
                     IMITFVAGIIGIVFGIGLSKLIESFAVQYELKAVMSLPSILIAFGVSSGIGLFFGIYP
                     ANKASKLSPIEALRYE"
     gene            9446..10594
                     /locus_tag="PLU28_01225"
     CDS             9446..10594
                     /locus_tag="PLU28_01225"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOG33717.1"
                     /translation="MKAILIVDDISINRKLIKAVISKNMAELNFYEASDGVQALEIIY
                     HNQVDLIFLDLLMPKLDGFGFLKEIKSSDRYSNIPVIVSSAIDEIEYVKKALELGADD
                     YFTKPFTSEQIEFILPLKVKNALKEVEHIRTIMELNSMMQEEMELANIFQHMIINNTK
                     ELKVAQMYGDYLPASKVGGDFFDCIENQDGLWFIIADVSGHGVAASMISSMVKVMFEN
                     YIKLEKTPAGVIKRMNDFFCDSIKDNLYFSFTAFIGNIKNNCLTFCNAGHPYPLVYKA
                     VSKDFFVIEENGFIVGIMKEAEYFQMQTEVAAGDFIILYSDGFQNNADDDQLTVRVWV
                     KQLIGFMNKLDMNLMTAEDTVWKIKQNFLKCQGKRCSDDTSIMVLKIL"
     gene            10617..10937
                     /locus_tag="PLU28_01230"
     CDS             10617..10937
                     /locus_tag="PLU28_01230"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HOG33718.1"
                     /translation="MTENELCEKLVLRYGLTKEEALDIIKDFRQLFIQKLEELKNSLY
                     VLDYTQIKNCAHSLKGASANIMYDEVAVLFSDIEKAAISQNEDSIAELVRRAEAFQKS
                     GGIL"
     gene            10934..11689
                     /locus_tag="PLU28_01235"
     CDS             10934..11689
                     /locus_tag="PLU28_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HOG33719.1"
                     /translation="MNSISEKAVIGDNVKIGRNVVIDDGVVIGENSVIGDLVIIHEGT
                     KIGRSSVIGDSTVLGKRPFKASNSAVTKDVLLDPLAIGECVTVGAGCILYAGALIQSH
                     VFIGDLASVREEVSIGEFTIIGRGVSVENKTVIGRYVKVETNAYITAISVIEDYCFIA
                     PEVTFTNDNFLGRTEERKKYFKGPTLKKGARIGANSTILPGVVIAEDTLVAAASVVTR
                     STLAGKVYIGSPAKVLRDVPQEQLLKNQSYYKE"
     gene            11809..13812
                     /locus_tag="PLU28_01240"
     CDS             11809..13812
                     /locus_tag="PLU28_01240"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG33720.1"
                     /translation="MEKNGMSIASKFSFYLGAIVALSVFVVGMIGVLWSYDLRKKDFI
                     SVYESSTANLSELMSLELQEKDFQGMEKAADTFFLDDSVKSITVYDVHDSIVFIKEKK
                     VPSNHLSVSKTIFYGNKALGKLKVSFGYDALISDFVDSIAMILLLVVVLIAVIQFSSS
                     FLFSRFIKKPLDKLMVYIQNISNGRYDEKIEKSCGEFLKIEESFAKMVLEIKNRETKL
                     RESSYRLERLDTALEVSKFAVEKSMIEAYWIKTDGSIVYANSSARKNLGYSEQEFGKL
                     TVFDIDPDLDEQLWSSHMEKIKDEQSTVFQRKQKRKDGSLYDVEVTSNYLVYKDKKYN
                     FSFVQDITERIKTQKRLMELNNNLEKLIQQRTMNLEKAYAQLDEANEGLVAMNQELLA
                     MNQELQAALDELGKTQKSLIESEKMAALGKLVGGIAHEINTPLGNGLVGSTYISDENR
                     KILNMHSKGELTKSCFEEFLRKNIETSDIIVLNLKKAAGLVVSLKEISVDQSSQEKRQ
                     FNLKKYLEDIVLTMNYPLKKNGHRTEIDCDENLDIESFPGALSQIITNLVSNSVIHGF
                     EGLEKRTIKIKAVLITDQSDKNKQMIELVYSDNGHGIIQQDIEKIYDPFFTTNRTKGN
                     TGLGLSIVYNLVTQTLKGSIKCQSIKNEGTVFTVSFPVSSVSR"
     gene            complement(13802..14887)
                     /locus_tag="PLU28_01245"
     CDS             complement(13802..14887)
                     /locus_tag="PLU28_01245"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOG33721.1"
                     /translation="MDDFLSFAQETQNTCGYTKEAWKILIVDDDVMIHKITKIVLKDM
                     TFRNKNIEFLSAYSERQAREVLFREDDIAVVLLDVVMEENDSGLKLVEYIRNELKNSM
                     LRIILRTGHPGSAPEEKVVLEYDINDYKEKTELTKGKLFTSIINALRSYDDMVQLDLK
                     RKELKETQKDIIMTLSEIAETRSKETGNHVKRVSEFAQLIGKKYGFSDEDNEILKNAA
                     ALHDIGKIVIADSILLAPRQLTAVEFDIMKNHTVYGYDLLKSSKQPMLQSASVVALQH
                     HERYDGKGYPNGLSKDEIHIFARIVSIADVFDALSTDRVYRKAWTLANTLEYMKRERD
                     RQFDPFLLDIFINSIDTVKSIMNKLND"
     gene            complement(14892..17219)
                     /locus_tag="PLU28_01250"
     CDS             complement(14892..17219)
                     /locus_tag="PLU28_01250"
                     /inference="COORDINATES: protein motif:HMM:NF021619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PocR ligand-binding domain-containing protein"
                     /protein_id="HOG33722.1"
                     /translation="MKYLLEDLLDIIALKKVLTNFYEFSEVPVTLINSAGRPLISIGW
                     IDICTKFHRKNPFSSARCAESDSELFNSMLKENSQVLKKCSNGLLELGSKITIDNVFM
                     GSIVVGQFLTEKPDINFFMEQARNYGFNEPEYILAVKKIPLISHEKINAIVQFVTSLT
                     FMIAQVGYKRLKELELINIMNITQDRLTNVEGFYSQLLDILPDPCIIMGLSGEIRTAS
                     KKALQLFDYTLESFSKMKITQVIEPQSIKDASDDLAALKSCLEITSREYCLKTSKGKI
                     FYAAVNSKLLYDIHGSPNAIVCSLRKIENFSENDESSLKVQISLSNLFDINLIAALYM
                     KLQLRSLTAENKDALYEANGFSWSSTDGKESTFHLKDAILAAAQAYRPKIGKLNITME
                     NSSDMVPDFIISDFHALARILYDIFAVIERISGNQAIKINVSTFNLTPQKLGISFTLD
                     SSVFSIDEFSGTYRFSWTDYSLADDLFFRLPYLKALVSSMKPLSGEMQVLFTKSLSRL
                     TLEFAFKNFQNDTALSSECFENQSGGKKSMSDLNVLVAEDDFINQKLIGNILAKEKIS
                     CFIVSDGQKAVDEYIRNPSYDFILMDIQMPVMNGIDASNAIRKINPTVPIVALTADLS
                     SEDKDMLKAARIDKLLVKPFQTSDLLETLKIINICTSSSEESLKELIGKVQEETGLVE
                     SEVQELLGDYVKYTSSEFEILGEMVEKNDFEGISKLSHKLKGSSLSLRISNIAEILQK
                     MDEDAKNKNNLNFQDYLDELDIYLKAIKNGLSGQR"
     gene            complement(17391..19307)
                     /gene="htpG"
                     /locus_tag="PLU28_01255"
     CDS             complement(17391..19307)
                     /gene="htpG"
                     /locus_tag="PLU28_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HOG33723.1"
                     /translation="MAEKMQFQAETRQLLDLMINSIYTHKEIFLRELISNASDALDKA
                     RFRSLTDTSILADDTELKILLEIDKEAGCLKVSDNGIGMSYDEVIQNIGTIAKSGSKA
                     FIEKMKLTQASADGLELIGQFGVGFYSAFMVAKKVTIMTKSIDSQERAVLWESEGDGT
                     YTIDYCDRLNRGTTIVIDLRDEFKNDANGLNLLDQYTISDLVKKYSDYIRYPVIMDYE
                     VEEKIKDDADSTKEKTVTRKIPKTLNSLLPLWEKDKKNISQDEYNQLYKDLFHDWNDP
                     IQVVHFKGEGTVQFTALVYIPSKAPYDFYTKDFQRGVSLYSKHVFIMEKYEDLLPEYL
                     GFIKGLVDSPDFSLNISRELLQQGKQLQGIKKSLEKNIIQALKSMLENDRKKYEGFWK
                     EFSRAIKAGIYMNYENKEKLSELLMFDSSSDVGLTTLKEYVSRMKEGQEYIYYAFAKD
                     RSAAQKLPQIEGLIDKGFEVLFIYENMDELVLDFMSEFNTKKIQSASKEEEKPSDKQD
                     KTENAEQSDILQFIKEHLKNTVSDVRISSRLKNSPSCIVSSKDGISMAVEQLIKQMGE
                     NQDLSELKAKKILEINPDHKMYAAIKASFEQDKTSQKLKNLIDVIYAQALISQGLAPE
                     DPSSFIEKISELIS"
     gene            complement(19376..19810)
                     /locus_tag="PLU28_01260"
     CDS             complement(19376..19810)
                     /locus_tag="PLU28_01260"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOG33724.1"
                     /translation="MTAYIRYLPADDECGFCSSVLNGQEYDISELMAKDSTYRGISPD
                     TDIYEKDEKIIFETELPGLSKNDIKIKVQEDILSVSAQKSVDASRKFLRKERVYGKLE
                     RTFRLPKDYDVNSLNARYENGVLTLELSKIKNDNKVREVQVL"
BASE COUNT         6157 a         3435 c         4162 g         6201 t
ORIGIN      
        1 tgcttggtct tcttattgag gaggtatgga aggaatgctg aaaaaaatag aagcttattt
       61 catgaaggaa attgctgata aagcaggtct gatagccatt ttatccctgc ttctgtttat
      121 accaaacttc tacggacgtg ttatattcct gcttcttctt atctgtctta tgctgccttt
      181 tgatatacac aacaaaaggt tttcgctctt aatatccctg cctttttcgc acagggaaat
      241 atttttatcc ggatttgttt tcgtacttgc agtaagcatt tcaacccagt ttataggcgg
      301 agccttactg ggagttaaga tagcctatct tatggtggag tgtttaaaga gtgttatttt
      361 ttgtgctttt tattacggag tttctatggt ggctgtttta tacggtttgg ataatcttgg
      421 catcccgctt ttagtatttt ttgccgatgc catattcgga gctataggac ctaagtggag
      481 taatttttac agctatataa gtcctgttca taacggcaat cttctgtttt cagcggtgtt
      541 tgcagcggta gtctttgtct tttcatttta tctgtttgaa aggaagggaa ttcagaaatg
      601 ataataaata ttgaaaatgt caataaaagt tttggcagta agcatattct caacaatata
      661 tctttttcag tccaggaagg ggagatcttt gcccttatag gaccaaacgg cgcaggtaaa
      721 accacaacca tcagatgtat tttcggggag ctgatgcctg aaagcggtaa agttgaggtt
      781 ttttcctctc cggtcagttc aaagctgaag gaaagaatag ctgttatgac ggaagaccgt
      841 cttaccttta agcggtttaa gggagccgat tatcttaagc tgtggaagat gctttatcca
      901 agatggaatg aaagcatttt tgccagtttt atgatgcatt acagatttaa tcttgaagac
      961 agagtggaaa ccttttcgat gggtatgaag actctgtttt atcttgcatt ggatatggca
     1021 agcggagctg acctgctcat tcttgacgaa ccaacacaga atttagatcc ggtaataaga
     1081 catgaggttc ttaatatgct ccatgatttt gttctgagtg agaataagac catagttatc
     1141 tcttcacacg agatctacga gcttgaggag attgcgtctt cttttgccat aataaacgaa
     1201 ggaaaagttc tttatactga cactatagac gatgccaagg catcacacag aattgtggaa
     1261 aaaggtgaaa ggattccgca cggggatgtt ataggtctgc tgaatgaagg aactcttata
     1321 agaacctccg aggatgcggg gagatatccc aacttcaagg agatagcgct tgcatatctt
     1381 cagggtggca aagcctttaa tccgtttgaa aggctgtgag gtatgatcag tccacaaaac
     1441 aagagtctga aagtgctttt tctgtttctc ctggtcggaa ctgttttttt cggaaatact
     1501 gtgatattta caccgcctaa agagcttctt acacttaaaa ccaagaactt tgagtatatt
     1561 tatgaagaga agctttactt ttatgttcag gatataatgg agtcggccga ttctgtatat
     1621 gaagactatg cttcttttta cggggtaagg cctgagggta taaaggttta catgcttgat
     1681 gatgttgatt atgtaaatgc ttttgccgta ggatttgaca ataagataag aatgtatgtc
     1741 aatacggcgg acttttcaag cggacttttg agttccaact ttctttcaaa ccgcatgatt
     1801 ttttgccatg aactgaatca tcttttttac agtaacatgg tcagcaatcc ttttatttct
     1861 tggataccga atcagaccat aaagaaaata tctaacatgt cttctcagcc cagttatctt
     1921 catgaggggc tgagtattta tatggagagc aagtactttg gcggaaggtt tgaaaatgat
     1981 ctttttaata tgtaccttaa agctgaagtc ttgagtccta agtaccccag atacgagctg
     2041 ggatcaggcg cacaggttaa ggtgtggtcc ccggcaggct tcaactatat gtacggagct
     2101 ttatttacaa aagaaatagc cggcttttat tctgaagaag tactaaggga gatagtcaga
     2161 ttgacttcaa gcagtttttt ttccagtata tccggtgctt ttgaaaaggt aaccggagat
     2221 aagtggagcg agtttcttca gaaaattaag ttaaagtata tagaacagat gagtatcgtt
     2281 ggtgataacg gaaatattca aaaatatgag aaactcggaa aagagtatga gcggacggga
     2341 aacctcagaa ctgacggtaa aaatctatat gcttataagg ttatgccttt ttccagagca
     2401 ggtgtatata aaaacgaaga gcttattatt ccggaagtaa gcaagtttga tgtttcgtca
     2461 aacggagagg taatattttc aaaaactata agtaatgacg gcaacgcttt taacagtctt
     2521 tatattaaat gtgactgtcc catatcagat actctcatag cccaaagagt tcttaatttt
     2581 gggtttgcgg atagccgaac ggtagtatac accgtcaatg acaaaggact gagcggagtt
     2641 tttgtatatg atctgattga gaagaagtct tctcagatcc ttggctggga caagtacgtt
     2701 atgaatagcg tgtgcggtac acaagaggat atctacttat cactgacata cgacggacag
     2761 tcggacatat accggtatga cagaaggaac gcatctatgc aaagacttac agacgataaa
     2821 gagtatgaag ctgatctgtc atatgccaag ggaaagcttt tttacactaa gaactataac
     2881 gggattttta atatattcag tcttgatctg caggacatgg ctgtcaggca gatgaccttt
     2941 tcggagatgg gagctttctc tccggcagtt ttgggagatc agctttactt tctttcatat
     3001 gactgggagg gttattatat ttccaaaaca gatcctgctc tatccaccga gtcagaaaca
     3061 ctttctgcca aagctttcat gtttgaaagc accggatttg tgggaaaaaa cgatctttct
     3121 gccgagaagt actttgaaaa tccgcagcat ataattgttt atccgtatgc agaactctct
     3181 gatgatatct tagagatgga tctttctaag ctgactctta attacggaat gggcgtggca
     3241 gtaatttccg atgcttacaa ctatatgatg aactatagtc ttgtctttaa aaaagatgca
     3301 ttaaaacata acctggagct gtattttaac tctttggcgg cagtggatct aaagcttatc
     3361 tacgaaaaaa acattcattc agctcttgaa gtcagtaaag acttttcttt ttaccttaaa
     3421 gacatgtact ttctgaagtt ggcagctacc ggatcagctg atggaataac tcctgaaaaa
     3481 gtaagcgtgc aggctcagct gtttcaaaat ccatactcca taaacaagat aagtctatac
     3541 gatgttgctt tgagcggatc ctatttatac gacaatacca aaaattctca gtatttcagt
     3601 gtaggaatat ttaaaccgtt ttatctgtat aacttcagac tgacaccctt ctttgagtat
     3661 gacagtcttg ggaatacttt ggcgggagca acactttacg gagatctata ctatccccac
     3721 tggaatattt ttgacggcaa aagaggttta aacggttttt cagtcgaaag caccttaaat
     3781 tataacttta aagatcggaa gatgtcatgt tcggtctatt tcatactaga ccttaaaaca
     3841 tcatactggg ctaattattc ttataaagta ccggttttta agatgccttt aagtaattag
     3901 ctgtaatatg ctaaaagata atttaaaaga gaggagaaac ttaaatgaaa aaaacatttt
     3961 tcatcattat gattttggga tttgcagtaa tttcattcac caatccgata ggtgaactgt
     4021 tcaaaagtgc tttgaacagc aataagggta ttaagatgac tatgcttaat tttgagagtg
     4081 cacagatgga atatgaaaaa gctcttatag agtcatcaaa taaaaaggct cttctttcgg
     4141 cggagctttc tatgctgaat gaaagatcct catatagaaa aggtcaggct gatttttatt
     4201 cagaggttgt cgatgctttc tttgccgtaa aatcggctca gatagagctt gaaatatcgc
     4261 agctccagct tggaattgtt caaaatgatc ataagaacgc taagctttcg tttgaaaaag
     4321 gtatgatatc gctcaactca ctcaaagatt ctgaggtggc agttatagat gcccaaagtc
     4381 aggaagaaaa agcacgaaag gattacaatg atgcgcttac agcttttaaa agagctaccg
     4441 gtacggagta tgccgattac gaagtaaagc cgtgcgacta taaagagtgc ggtttttcat
     4501 atgaagcgtt caaagaaaat gatctgagcc taaaagccca gcagataaac cttcagcttg
     4561 cagagtacga cctgcagaac ctttcatcta actcatccta ctatgaaaaa agaggacttg
     4621 agataaaact tgagatatcg cagatgaatc tcaaaaataa agaggaaagt gtatctgaaa
     4681 gttatgaaac tgctttggca atggtcagtt ctctttattc ctctttgcag agcagtggtg
     4741 tgaagcttca gattaagcag agtgtttacg aagacagcca gaagcgtttt gaaaaaggct
     4801 ttgtttctga aaaagacaac attaatcaaa aaatatcgta tatgagtgct caaaaggcat
     4861 ataacgaaaa cataaaatca tatatcaaag cgcttgtctc tttaattttt aactgtggtc
     4921 tttctgtcgg ggaggcagtt ctttgaaaaa aattttcata gctatgttga tttttattgt
     4981 aactgttgtt tatggcggga taacagaaat ttataatgaa agagtggaaa agagtactgc
     5041 ctttatgttg tctgaaaact cttttaacca ggcagtacgg actcttgatg aaaagaaatc
     5101 cttctggata cctggattaa ccgcagggtt cgatcagata atttttggaa aggacggtct
     5161 tgaaaacaag aatattgtta acgggcataa aagtataaga ctaaacttaa aagccaactt
     5221 tgtcaagatt ttgggtgcga gcataggtgt aagtcttccg tatgtatacg atcttaccga
     5281 ttattctggg aagtttgaaa acttcaatct taccgtttca agacctttgt tcacggagtt
     5341 ttatcttgag gttccgcagg ctgagctgag tgttatgcag agcaaaaatg caatggaaaa
     5401 aaccagatgg gatattttca gccagctttt acaggatatt ttcaattatc ataatgcgct
     5461 ttcaacaaga gaacttatca acggaagaac ttatgtgact gagatactat atgaaaatga
     5521 aaatgataat gaaaagaaaa aaacttacag acaacagctt accgagctga ataagctgag
     5581 tataaacaac gagcttgttc ttaacgggct gaatcttgaa tactgtgcag agctttacag
     5641 ccaggcagtt cagatttgtg aaaagatggt tctgatttct gaggactgca cacaggtcaa
     5701 tttcaatgga acggaacttc cttcagtatt acttaaggag gatatagccc gccgtcagaa
     5761 ggaaatgtgg tttttaccgt atcttcctaa tccggtgata tcttttggca tagatctttt
     5821 agacagttta aaatggcatg taggattgag ctttgaatat tcgttttttg aaaaaggtac
     5881 ggtatcaagc aagtctgttg aaagctccaa caggatttca cagtacgaga tagctgttct
     5941 acagactgct aatgcaaaag agagtacaca gcacagtatt gagaagttaa aaaaggatct
     6001 tgaaatttat aaaatggagg tatttgcaag taaggctaat atggatgatt cacttgcaga
     6061 actcgctaaa aacaagctcc ttatggaaaa aggatatctt actaaggaaa agtatgatct
     6121 gggatcttat gaacatatgt caaaaaaact tgattatgaa aaaactttgc agagcatgta
     6181 tctgacctgc gttaaacttt tgcaggagta tggattgcct gcaggaggag gtacgctttg
     6241 aaaaaacgca gtaaggtcat tatttggatt gtagttttaa ttttaatagt atttgtggca
     6301 ataaaattct tcagaggcgg cagtactgct gctgatgccg gtcaaaacca gttaactcag
     6361 aatggaagtg tcggtggcta ttttttaacc tatagggttg aaaaaaaaga aatacctgat
     6421 gcggttgaac tgtttggaag tgttgatgcc gatgtttata aaataacttc caagatgaat
     6481 gaggagatag agcaggtcta tgtaaaacag ggagataccg ttaaaattgg tgatcctctg
     6541 gtgaggctta gtgaaaccgg gctgcgctca aagtatcttg ctgcctacag ggattatgaa
     6601 agctcactta ccggtgctcc tattgatgtt cagcagaaaa aaatagattt ggatctggct
     6661 aaaaaaaatc tggataatgt gataataaaa tcccccgcca acggaacagt aaaaagtgtt
     6721 tccgctaaag ttggggagta ttcttcccaa agcagtgagc ttgtaagcat agtcgataca
     6781 tcgagtctta tggctaaggc ttccatagac gaactggaca tacctgtcat aaagaaaggt
     6841 agcagggtga taatagagtt ttcttcgttg aatgttaaga taagcggtta tataagttat
     6901 gtaagccctt cggctgcaaa ttcgggaggc atggtttctg tcccggtaga gatctctttt
     6961 gatgaggatg cgtcaaagta tggggtaata tccggcatga gctgcaaaat aaaagttctg
     7021 ctgatgcata aacagaatat acttgttgtt cccgtaagag ctgtctactc agatgagtct
     7081 ggcgagtatc ttctcgtgat ggatgaaaag ggtgaaaggc agaaagtata tattaaaacc
     7141 ggcgagcgca cagttaatgt ggttgaaata atagaaggaa taaaagaagg gacagagata
     7201 ataatagaag aaagtccgga tgtcccggtt ccggagaatc ttggaaaagg ttattatgca
     7261 tctgtttgta agcagggtgg atacgatgcc tgtaatagaa tttaaagaaa tctcaaaagt
     7321 ctataagatg ggcgatgttc aggtgaatgc cctaaaaagc atatcttttt caatagaaca
     7381 cggggactat gttattataa tggggccttc cggaagcggt aaatctactt tgcttcagat
     7441 actgggctgt cttgacagac cttcaagcgg cgaaatttcc atagacggta cacagacttc
     7501 aaagatgaac gaagatcagc ttgctaaagt gagaaatcgt aagataggct tcgtttttca
     7561 gggcttcaat ctgcttccca aacttactgc gcttgaaaat acaatgcttc cgatgatata
     7621 cggaaatgtt tacaagggta aggaaagaac agagcgggca aaagagcttt tacaggatgt
     7681 cggtctgaat gagcgtataa accaccgccc taatcagctt tctggaggac agcagcagcg
     7741 tgtggcaata gcgagggctc tttcaaataa tccttcaata attcttgccg atgagcctac
     7801 cggcaacctt gacactcaga gtggcaagga gatactcgaa atatttggca gactgaactc
     7861 atctggtaag accgtgatca tagtcactca tgatccggat atggcaaaaa aagggaacaa
     7921 gctgataaag ctgagggatg gtcttattga aaaaatagag gtgaccggta ttggaaatac
     7981 ttaaagagac tatgaggtca cttctgacca ataaaatgag gacctttctt tcgatgctcg
     8041 gaataataat cggagtcatg gcggtaataa ccgttactgc ccttgggcag ggaacaacca
     8101 gcagagtgag cgagatgatg aactcactgg gatcaaatgt aatacttgta gaacaggggt
     8161 atatgggagg ttatggcgga atgtcacttt catctgtcaa aaatgccctt tctatacagg
     8221 atgtagataa cataagaagc atggcaccaa gtgtaaagta tgtggttccg gttttaaacc
     8281 ccggtttgga gcttcagtat gaaaaaagaa gcgtttattc catgactata gcaagccgtc
     8341 cggagttttt tgagatgatg ggacttgaaa ctcaggccgg aaggatactt gataatgaag
     8401 atgaaaaaaa tgtcgctaaa gtatgtgtga taggttccga tcttgcgctt gaacttttta
     8461 agcaatctga tcccataggg aaaacagtat tcttaaatca gaactacggc gagtccgggc
     8521 gcaaaacatc tgttcaggtt ataggagtat tgaagaagac aggaagtaag ttttttttca
     8581 atgcagacag aacggttttt ttaccgtact caaccggaga tatgaggatt aagcagtcca
     8641 atggaactgt tgacagcata atcgtttctg ccaaagatgg actttctgcc aaagcgtgtc
     8701 ttgagataaa ctatgtgctg tacttgagac tgaagaatat acaaaaatac gtgatcactg
     8761 cacaggaatc tctttcccag atggttggac aggttaccgg agttataaat cttgttctca
     8821 tggcgattgc tgggatatct ttgcttgtag gcggcattgg tataatgaat attatgcttg
     8881 tttcagttac cgaaagaaca agggagatag gaattaaaaa ggccataggt gcatcacgta
     8941 aaaggattct tcttgagttt cttgttgaaa gcattatgat cacttttgta gcgggtatca
     9001 tagggatagt tttcggtatt ggtctttcga agcttatcga aagttttgct gttcagtatg
     9061 agctgaaagc tgtaatgtca ctgccttcaa ttcttattgc tttcggggtt tcgtctggta
     9121 taggcctgtt ttttggtatt tatcctgcca acaaggcgtc taaacttagc ccgatagagg
     9181 ctttaagata tgaatagagg tatgttttct tttttttcta aacatagctt tattttaacc
     9241 taaatgtaac taatgtgagt tatactgtat ataattataa gattagtatc tgataaaaaa
     9301 cttataaccc ataatagata gcttcatctc ttatatttaa atattagccc cacctccaag
     9361 ctgatccttg ggggtctttt tttatagatg aggtttaggg tataattaaa aataaaaagg
     9421 caggtttttc tatggaggaa agacagtgaa agctatactt attgtggatg atatttcaat
     9481 caaccgcaaa cttataaagg cagtaatatc aaagaatatg gcagaactga atttctatga
     9541 agcctctgat ggtgttcagg cccttgaaat catttatcat aaccaagtag atcttatttt
     9601 tctagatctt ttgatgccca agcttgatgg ttttgggttt ttgaaggaga taaaaagcag
     9661 tgatcgatac agtaatattc cagtaatagt tagttcagcc atagatgaga ttgaatatgt
     9721 aaaaaaagct cttgagctgg gggcagatga ctattttaca aagcccttta catctgaaca
     9781 gatagagttt atacttccgt taaaagtcaa aaatgctctt aaggaagttg agcatatcag
     9841 aaccattatg gagctaaaca gtatgatgca ggaggagatg gaacttgcca acatatttca
     9901 gcatatgata ataaataata ccaaagagct taaggtagct cagatgtacg gtgattatct
     9961 tccggcatcc aaggttggcg gcgatttttt tgactgtata gaaaaccagg atggactttg
    10021 gttcataata gccgatgttt ccggacatgg agtggctgct tcgatgatat cttccatggt
    10081 aaaggtcatg tttgaaaatt atatcaagtt agagaaaact cctgccgggg ttataaaaag
    10141 gatgaacgat tttttttgtg atagtataaa agacaatctg tactttagct ttacggcatt
    10201 tatagggaat ataaagaata actgtcttac cttctgcaac gcaggacatc catatcctct
    10261 ggtttacaaa gctgtatcca aggatttttt tgttatagag gaaaatgggt tcattgtagg
    10321 tattatgaag gaagcagaat attttcagat gcagacagag gtagctgcag gcgactttat
    10381 aattctgtac agcgatggat ttcaaaacaa tgctgatgat gaccagctta ccgtaagagt
    10441 ctgggtaaaa caactcatcg gttttatgaa caagcttgat atgaacttaa tgactgctga
    10501 agatacagtt tggaaaataa agcaaaattt tcttaagtgt cagggaaaaa gatgctccga
    10561 tgacaccagt atcatggttt taaaaattct ctagacttta tgatggagga gattttatga
    10621 cagaaaacga actttgcgaa aaattggttt tgagatacgg gcttacaaaa gaagaagctt
    10681 tggatataat aaaggatttt cgtcagttat ttattcaaaa gctagaggaa ctaaaaaatt
    10741 cattgtatgt gcttgattac acacagatca aaaactgtgc acactcactt aagggtgcaa
    10801 gtgccaatat aatgtacgat gaggttgcgg ttttgttttc tgatattgaa aaggcggcta
    10861 tttcacagaa tgaggacagc attgcggagc ttgtgcgcag ggcggaagca tttcaaaaat
    10921 cgggagggat attatgaaca gcatatcaga aaaggctgtt ataggagata acgttaaaat
    10981 cggaaggaat gttgtgattg atgatggtgt agtaataggt gaaaattcag ttataggcga
    11041 tttggtaatt atccatgaag gtactaaaat aggaaggtca agcgtgatag gcgatagtac
    11101 tgttttgggc aaaagacctt tcaaggcaag caactcggct gttaccaagg acgttttact
    11161 tgatcctctt gctataggag agtgcgttac ggtaggtgcg ggctgtatac tatatgctgg
    11221 agctttaata caaagccatg ttttcatagg tgatcttgca agcgtgagag aagaggtttc
    11281 catcggggag tttacgatta taggacgtgg tgtgagcgtt gaaaataaaa ctgttatagg
    11341 aaggtacgta aaagtcgaga ctaatgcata tatcacggca atatccgtta tagaggacta
    11401 ctgtttcatt gctccggagg ttacctttac caacgataat tttttgggga gaacagaaga
    11461 aagaaaaaaa tatttcaaag ggccgacatt gaaaaaaggt gcaagaatag gggctaactc
    11521 tactatactg cctggtgtgg tgattgctga ggataccctt gtagcagctg caagcgttgt
    11581 aaccagaagt accttggcag gtaaagttta tataggctca cccgccaaag tactcagaga
    11641 tgttccgcag gaacagcttt tgaagaatca gagctactat aaagaataag accggcaggt
    11701 cttattcttt tttttgtaag atatatttac cttcttaaca aaaaaacgta ttataaagta
    11761 gatatcatgt aaaatgtgtt tttaaaaata aaagtcagag gtaagcgaat ggaaaaaaac
    11821 ggtatgtcta tagcttcaaa gttttcattt tatcttggtg caatagttgc tttatctgtt
    11881 tttgttgttg gaatgatagg tgttttgtgg agctacgacc taagaaaaaa ggatttcata
    11941 agtgtgtatg aatcaagcac agcaaatctt tcagaactta tgtctttaga acttcaagag
    12001 aaggattttc aggggatgga aaaagctgcg gatacttttt ttcttgacga cagtgtgaaa
    12061 agcataactg tgtacgatgt ccacgatagt attgtgttca ttaaggaaaa aaaagtaccg
    12121 tctaatcatt tatccgtttc taaaaccata ttctatggga ataaggcact gggaaaacta
    12181 aaggtttctt ttggatatga tgcgctgata agcgattttg tcgacagtat agccatgatt
    12241 ttgttgcttg tggtggttct tatagcagtt atccagtttt catcttcatt tttgttttct
    12301 aggtttatta aaaaaccttt ggacaagctt atggtttata ttcagaatat ttctaacggc
    12361 agatatgatg aaaagattga gaagagttgc ggagagttcc taaagataga agaaagcttt
    12421 gcaaagatgg ttttggagat aaaaaaccgt gagacaaagc ttagagagag ctcttacaga
    12481 ctggaaaggc ttgatactgc attggaggtt tccaagtttg cggttgaaaa gtctatgata
    12541 gaagcttact ggataaaaac tgacggaagt atagtctatg caaatagttc agcgaggaag
    12601 aatcttggct acagtgaaca ggagttcgga aaactcactg tatttgatat agatccggat
    12661 ttggatgagc agctgtggag cagtcacatg gaaaaaataa aagatgagca aagcacggta
    12721 tttcagagaa agcagaaaag gaaagacggt tctctttacg atgttgaagt tacttcaaac
    12781 tatcttgtgt acaaagataa gaagtacaat ttttcttttg tacaggatat cacggaaaga
    12841 ataaaaactc agaaaagact tatggagctt aacaataatc ttgaaaagct tatacagcag
    12901 cggactatga atcttgaaaa agcttatgcg cagttggatg aagcgaacga gggtcttgtt
    12961 gcgatgaatc aggagctttt ggctatgaac caagagcttc aggcagctct ggatgaactg
    13021 ggaaaaacac agaaaagtct tattgaaagc gaaaagatgg ctgctctggg taagcttgtc
    13081 ggtgggatag cccacgagat aaacacacct cttggaaacg gtctggtggg aagtacctat
    13141 atatctgacg agaacaggaa aatactgaat atgcacagta aaggagaact tacaaaatcc
    13201 tgttttgagg agttcctgag gaaaaatatt gaaacttcgg atataatagt tttgaatctt
    13261 aaaaaggctg ccggtttggt agtcagtctc aaggagatct ctgtggatca gtccagtcag
    13321 gaaaaaaggc agtttaactt aaaaaagtat cttgaggata tagttcttac catgaactat
    13381 cctctgaaga aaaatggtca ccgtacagaa atagactgtg atgaaaatct tgatatagaa
    13441 agcttcccag gagctttgtc gcaaataatt accaaccttg tttccaactc ggttattcat
    13501 ggatttgaag ggctcgagaa acgtacgata aaaataaaag ctgttcttat aaccgatcag
    13561 tctgataaga ataaacagat gattgaactg gtttacagcg ataacggtca cgggataatt
    13621 cagcaggata tagaaaaaat atacgatccg ttttttacaa ctaacaggac taaaggcaat
    13681 accggtctgg gactaagcat agtttataac cttgtcaccc agacgctcaa gggcagtatt
    13741 aaatgtcaaa gtatcaaaaa cgaaggaaca gtctttaccg tctcatttcc tgtatcttct
    13801 gtcagtcgtt gagcttattc attatggact taactgtatc tatactgttt ataaaaatgt
    13861 ccagaaggaa cggatcaaac tgtctgtcac gttcacgttt catatattcc agtgtattgg
    13921 caagagtcca tgcctttctg tataccctgt cagtgctaag tgcatcaaag acatcggcta
    13981 tggacacgat acgtgcaaat atatgtattt cgtctttgga aagaccgttt ggataacctt
    14041 ttccgtcata cctttcatgg tgctgcagag caactactga agcactttga agcatcggtt
    14101 gttttgaact tttcagaaga tcatacccat aaacggtatg atttttcatt atatcaaact
    14161 ccacagctgt caactgtctg ggagccagaa gaatactgtc agctattact attttgccta
    14221 tatcgtgcag agctgcggca tttttaagta tttcattgtc ttcatctgaa aaaccgtatt
    14281 tttttcctat tagctgtgca aattctgata ctctttttac atgatttcct gtttccttcg
    14341 aacgggtttc agctatttcc gaaagtgtca ttattatatc tttttgagtc tctttcagtt
    14401 ctttacgttt taaatcaagc tgcaccatat catcataact tctgagagcg tttataattg
    14461 aagtaaaaag cttgcctttg gtcagctctg ttttttcttt gtaatcgttt atatcgtatt
    14521 caagcacaac tttttcttcc ggtgccgaac caggatgtcc ggttctgaga attatacgga
    14581 gcatactgtt tttcagttca ttacgtatgt actctaccag cttaagaccg gagtcgtttt
    14641 cctccatcac tacatctaaa agcactacgg caatatcgtc ttccctgaaa agtacttctc
    14701 ttgcctgcct ttcactgtaa gcgctgagaa attcgatgtt tttatttctg aaagtcatat
    14761 ccttaagcac tattttggtt attttatgta tcatcacatc atcatcaact atgagaattt
    14821 tccatgcttc tttggtataa ccgcaagtgt tttgggtttc ttgtgcaaag ctcagaaagt
    14881 catccattat gtcacctctg tccactaagc ccgtttttta tggctttaag atatatgtcc
    14941 agttcatcaa gatagtcctg gaaattaaga ttgtttttat tctttgcatc ttcatccatt
    15001 ttctgtaaaa tttcagcaat atttgatatt cttagagaaa gggatgagcc tttcaatttg
    15061 tgagaaagct ttgaaatacc ttcaaaatca tttttttcaa ccatctctcc gagaatttca
    15121 aattcagatg aagtgtattt gacatagtca ccaagcaact cctgaacctc actttccaca
    15181 agtcctgttt cttcctgaac ctttccaata agctctttca ggctttcttc tgaactgctg
    15241 gtgcagatat taatgatttt aagtgtttca agaaggtcac ttgtctgaaa aggttttacc
    15301 aaaagcttat caattcgggc agctttgagc atgtctttat cttctgacga aagatcagcg
    15361 gtaagagcaa caataggaac cgttggattt atttttctta tggcgttact ggcatctatg
    15421 ccgttcataa caggcatctg tatatccatt aaaataaaat cgtaagaagg attgcggata
    15481 tactcatcta cagccttctg tccatcggat acaatgaagc aggatatttt ttctttagca
    15541 agaatattgc cgataagttt ttggttaatg aaatcatctt cagcaacaag aacattcaag
    15601 tcagacatac tctttttccc tccggactga ttttcaaagc attcagaaga taaagctgtg
    15661 tcattttgga agtttttgaa agcaaactcc agtgttaatc tgcttaatga tttagtgaaa
    15721 agcacttgca tctctccaga taaaggcttc atagaagaaa cgagagcttt aagatacgga
    15781 agccggaaaa agagatcatc agccaagcta taatctgtcc aagaaaaacg atacgttcct
    15841 gaaaactcgt caatggaaaa aaccgaactg tcaagtgtaa aagatatacc caacttctgg
    15901 ggagttaaat tgaaagttga aacatttatt tttatggctt gattgcctga gattctttca
    15961 ataacggcaa aaatatcgta aagtattcgt gccaaagcat gaaaatcgga gataatgaaa
    16021 tccggtacca tatcgctgct gttttccatg gtgatgttaa gcttgcctat cttgggtctg
    16081 tatgcctggg cggctgctaa tatcgcatcc ttaaggtgga acgtgctttc ttttccgtcc
    16141 gtagaagacc acgaaaaacc attagcttca taaagagcat ctttgttttc agctgtcagg
    16201 ctgcgaagct gaagcttcat atacaatgcc gcaattaagt ttatatcaaa gagattggaa
    16261 agtgaaatct gtactttcaa agagctttca tcattttcag aaaaattctc tattttgcgc
    16321 aatgaacata ctatagcgtt cggagagcca tggatatcat aaagtaattt ggagtttaca
    16381 gcggcataga atatctttcc ttttgaggtt ttaaggcagt actctctgga agttatttca
    16441 aggcatgact ttaaagcagc aagatcgtca gaggcatctt tgatactttg cggttcaata
    16501 acctgggtaa tcttcatttt tgaaaaggat tccaaggtat aatcaaacag ttgaagagcc
    16561 tttttggatg ctgtcctaat ctctcccgac agccccataa ttatacatgg atcgggcagg
    16621 atatcaagaa gctggctgta aaatccttca acgtttgtca gtctgtcctg ggtaatgttc
    16681 atgatattta tcagttcaag ttcttttaag cgtttatagc caacctgggc tatcataaag
    16741 gtcaaacttg tcacgaactg aactatagca ttgatttttt catgagatat taacggaatc
    16801 ttttttacgg caagaatata ttcgggctca ttaaagccgt agttccttgc ctgttccatg
    16861 aagaagttta tatccggttt ttccgtaagg aactgtccga caacgatgct tcccatgaaa
    16921 acattatcga tggttatctt tgaacccagt tcaagaagac cgttactgca tttttttaac
    16981 acctgagaat tttccttgag cattgaatta aaaagctcag aatcactttc cgcacatcta
    17041 gcggaagaaa aaggattttt cctatggaat tttgtacaga tatcaatcca gcctatgctt
    17101 atcagagggc ggccggcaga gtttatcaaa gttaccggaa cttccgagaa ctcataaaaa
    17161 ttagtaagca cttttttaag tgctattata tctaagagat cttcaagaag atacttcatt
    17221 atgcacccac cctgaacaaa aaaataaaag caagcttata taatatttta ccagattatt
    17281 aactaaaaaa taaattttgt tttcaaagca tactaaaaag atagcatcat aaaaataaac
    17341 aacccccttt tatcaggtga aatatctggt aaaaggggtt tttagatgtg tcaggaaatt
    17401 aactcggaaa ttttttcaat aaaagacgaa ggatcttcag gtgcaagtcc ctgagatata
    17461 agcgcctgcg cataaattac atctataaga ttcttaagct tctgagaggt tttatcctgt
    17521 tcaaaagatg cttttatggc ggcgtacatt ttatgatcgg gatttatttc aagaattttt
    17581 ttagctttta gttcagatag atcctgattt tcacccatct gtttgatcaa ctgttcaaca
    17641 gccatactta ttccatcttt gctgcttact atgcatgaag gactattctt taacctggaa
    17701 cttatacgta catctgagac agtattttta agatgttcct ttatgaactg caatatatct
    17761 gactgctctg cgttttctgt cttatcctgt ttatctgaag gcttttcctc ttccttactg
    17821 gctgactgaa tcttttttgt attaaattcc gacatgaagt cgagcactag ctcatccata
    17881 ttttcatata taaaaagtac ttcaaagccc ttatcaatca aaccttctat ttgcggaagc
    17941 ttttgtgccg cacttctgtc tttggcaaaa gcgtagtaaa tatactcctg cccttctttc
    18001 atacgcgaaa catactcttt cagagttgtc agtcctacat ccgatgaact gtcaaacata
    18061 agcagctccg aaagtttttc cttgttttca tagttcatat aaattccggc ttttattgct
    18121 cttgaaaact ccttccaaaa tccttcatat ttttttctgt cattttcaag cattgatttt
    18181 aacgcctgaa taatattctt ttcaagagat tttttaattc cctggagctg ttttccttgt
    18241 tgcagaagct ctctggatat atttagtgaa aagtccggcg aatcaacaag tccttttata
    18301 aagccaagat actcaggaag aagatcttca tacttttcca ttataaatac atgctttgaa
    18361 tagagactca cacctctttg aaaatccttg gtataaaaat cataaggagc tttagatgga
    18421 atatatacaa gggcagtaaa ctgaaccgtg ccttcacctt tgaaatgcac aacctgtatt
    18481 ggatcgttcc aatcatggaa aagatccttg taaagctggt tgtactcatc ctgagatata
    18541 ttttttttat ctttttccca gaggggaagc aacgagttaa gggttttagg aatttttctt
    18601 gtaacggttt tttctttcgt actgtcagcg tcatccttta ttttttcctc tacttcatag
    18661 tccataataa ccgggtaccg tatgtagtcg gaatactttt tgacaaggtc gcttatagtg
    18721 tactggtcaa gaaggttcag accgtttgca tcgtttttaa actcatctct cagatcaata
    18781 actatagtgg ttccgcgatt tagcctgtca cagtagtcaa tagtataggt tccgtctcct
    18841 tcagactccc acaggacggc tctttcttgg gaatctatgg atttggtcat tatggttacc
    18901 ttttttgcta ccatgaaggc agagtagaaa cccactccaa actgtcctat aagttccaat
    18961 ccatccgcag atgcctgagt tagcttcatt ttttcaataa aggccttgga accggatttg
    19021 gctattgttc cgatgttctg gataacttca tcataactca ttcctattcc gttgtctgat
    19081 acttttagac atcctgcttc tttgtcaatt tccagaagta ttttcagttc agtatcatcc
    19141 gccaggatgc ttgtatcggt caaagaacga aatctagcct tatccaaagc atcagaagcg
    19201 tttgatataa gctctctaag gaaaatttcc ttatgagtgt atatagagtt gatcataaga
    19261 tccagaagtt gcctagtttc agcttgaaat tgcatttttt cagccattgt ttctttcctc
    19321 ctgtctattt ttatgaccaa aaaaggacct ctcgagtcct ttttggttta ttctgttata
    19381 atacctgaac ttcacgtacc ttgttatcgt tttttatctt tgaaagctct agtgtaagca
    19441 ctccgttttc atatctggca ttaagagagt tgacatcata atccttaggc aatctgaagg
    19501 ttcgttccag ttttccgtaa acacgttctt ttcggagaaa ctttctggag gcatcaacgc
    19561 ttttctgagc cgatactgat aagatatctt cttgaacctt tatctttata tcatttttag
    19621 aaagtcctgg cagttccgtt tcaaaaatta tcttttcatc tttttcatat atatcggtat
    19681 caggtgagat tccgcgataa gttgaatctt ttgccattaa ttctgaaata tcgtactcct
    19741 gtccgttcaa tactgaagaa caaaaaccgc actcgtcatc tgccggtaag tatcttatgt
    19801 atgctgtcat atatatttcc tcccatctca tatttttatg taatacaata atatcattat
    19861 cactcaatat tgtcaagtgc taatgtaaaa taaagaaaat caagataaat atattctata
    19921 aataagaaga tagcaggtaa atatattcgt aattt
//
LOCUS       DAOSHR010000012        19447 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_2268_length_19447_cov_5.073432, whole genome shotgun sequence.
ACCESSION   DAOSHR010000012 DAOSHR010000000
VERSION     DAOSHR010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 19447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19447
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2268_length_19447_cov_5.073432"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="PLU28_01265"
     CDS             complement(<1..245)
                     /locus_tag="PLU28_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOG33725.1"
                     /translation="MMNVVSLILAGGQGTRLGVITNEIAKPAVPFAGKYRMIDFTVSN
                     CVNSGITKVGVVTQYMPHELMEHLGIGKPWDLDIKGGG"
     gene            complement(258..1376)
                     /gene="glgD"
                     /locus_tag="PLU28_01270"
     CDS             complement(258..1376)
                     /gene="glgD"
                     /locus_tag="PLU28_01270"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HOG33726.1"
                     /translation="MRVLGLILCGSNQDNLKPLTRIRTSAAVPMFGKYRAIDFTLSNM
                     VNSGISKVGVLTQYNPRSLMDHLAGGKEWNLDRKRGGIFILQPFMVGSGESIYYKGTA
                     DAIFQNISFLRRGDEDFVLIGSGDHIYNMSYMDLFKYHIRKGADITVISSKICREDTR
                     ICDYGQLAVDNDGRISEFVEKPTDCIYEDISMGVYFINKALLLELLYSSVPNNGYDMV
                     RDIIIPNISKLRVYAYKHSGYWRNIKKSIKEYYDTNMDILKREVRKELFYSGRKIYTK
                     LKDYAPPKININACVSNSLIADGCIVNGQVKNSIISRGVIIKAGASVENSIIMEDSVI
                     EEGAILKNAILDKSVTLRNAKRIEGIGEILTIEKDSVI"
     gene            complement(1389..2555)
                     /locus_tag="PLU28_01275"
     CDS             complement(1389..2555)
                     /locus_tag="PLU28_01275"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HOG33727.1"
                     /translation="MTVVTLKRGKERKVLNGYPWIFSDETFSVVGDKKNGEICTVFSD
                     SMKFLGKGFFSSSNIAVRMLVLNDDEIDYEFFYSRVLKAFELRKAIMNNLDSCRIFHG
                     EADGVPGLIADKYNDYIVIQLRNVGVENKKAQIIEAFAKVYAPVGIYERSDFETGSAE
                     NIKRNKGVVFGKEPEHTLIVHEHGLKYNVDIINGQKTGFFFDQKDSRFFVRQFVDTSS
                     VCLDAYTFTGGFAMNMAVSGAKKVYAVDKDEQSLEMAKKNVDINNLGNIEFFSGTYEK
                     FMQEYSGEDFDVVILDPPSLIKKKEERRKGLDIFKGIVKLIVPKIKTNGIVCLCSCAY
                     QADTDMLIEASRMAFEGTGRSIQMVGFTFQSKDHPYIIQIPESFYLKCVWLKIK"
     gene            complement(2568..3011)
                     /gene="ndk"
                     /locus_tag="PLU28_01280"
     CDS             complement(2568..3011)
                     /gene="ndk"
                     /locus_tag="PLU28_01280"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HOG33728.1"
                     /translation="MEKEFLIIKPSTVKRGLIGSIISEVEKRGIKIIAMKMLWISRAQ
                     ACELYKEHEGRDYFEPLVSYAISGPVVALVVSAERAVQHIRHICGSTDPLKAEAASIR
                     GKYGLSVRKNVVHSSDSHENALREMKIFFNENELFEYEAVYEKEI"
     gene            complement(3021..3638)
                     /locus_tag="PLU28_01285"
     CDS             complement(3021..3638)
                     /locus_tag="PLU28_01285"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG33729.1"
                     /translation="MNEKIILTAEGIFYSYDKKNPVLKGVDIKVLKNEILTLYGPSGS
                     GKSTLIYILGGLLTQDAGKILYNIEDKKRKIGFVFQNYNMLNELTVFENCKVAQNIRS
                     FDDDRQIFDYAEKIGITDILYRYPAELSVGQLQRSAVLRSISGPVELVFCDEPTGSLD
                     SENKRNVFELFFQLKEYVSFFVVSHDEVSKKYADRVLNMSDGIIL"
     gene            complement(3631..4725)
                     /locus_tag="PLU28_01290"
     CDS             complement(3631..4725)
                     /locus_tag="PLU28_01290"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOG33730.1"
                     /translation="MTNKRFTDKAVFSLVKGFIKKSRKSFKFPTIAIAIGVWGLIVIT
                     SVVNGFDRLLIDSITRFYPHITVAGKSDLYFSGVKEKMFFNLDNSMVAFNGSFDYVQL
                     MDCDDMSFLGSSIISGQLENGAAIGKELSEYLNISIGDELTVFKIKGIIPIAKKYRVV
                     SLFKTGVYIFDSKFLVINDPDSVKNFTAYYFEDPLKADEYKNKYFSYSPAYTWKESNK
                     TLTQTVEIDGLIALLITVFIVLMAGFSVSNSITFSILTRKKEIGILKSLGMKNIDIQK
                     VFISESLIISVIGYLLGLAGGMLTVTMLDFFKIKIPSGIFYVDYLPVSLNLSIAGSSF
                     FIITGIVVFFSFLTTRMISGIDTLEALKDE"
     gene            complement(4715..5869)
                     /locus_tag="PLU28_01295"
     CDS             complement(4715..5869)
                     /locus_tag="PLU28_01295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HOG33731.1"
                     /translation="MRKYLSVFGSLFLVLILTTVLFSVKKAIVFKSDISLPYYFLIIG
                     MDSDTSQSVSRTDTIILAGINKGKIIFLPIPRDLMISSGNTEKRINALYSTLGKESFL
                     KTVSDLSGVQVKDYIIFNYSIFKTLGDEIAPVRVFVESNMSYTDYHQNLFINFKQGYN
                     DMDGEKLLYYMRFRNDEMGDLGRIQRQKKAFYSILDRAKNLGMTKLVDLIDIVKKETI
                     NTFSSSDLFKLYSYAKSSEIEFLEFPYIIKESYVISDNKRLEEVKAYLVKFEKNEEVK
                     KPSVMFIRNNSAYYIAFYNEIYAKWKAKGYLIYVPDVTFDFLEKSKSYILVKNQQKYP
                     NIKKDTQATFSKDFIDIPTAGETYFSIVKKVSSSFIDFTLYDAVVIIGDK"
     gene            complement(5877..6593)
                     /locus_tag="PLU28_01300"
     CDS             complement(5877..6593)
                     /locus_tag="PLU28_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG33732.1"
                     /translation="MARIKVKRGRKKGSFLKTVIFVVVIAVLLVGAFAVYNIYALNSS
                     SELKLAKSTYTFSYGDKIYFLRVLNDRNKILIVRAEDGLTFKDKLFSISKDNLKDSTN
                     SFLKEFELSSNLDFYIKVDENTVKKLNQSVKSQKEDMESFFEALKYRNSNIFDFFFVN
                     SLEGLIRKYDRDSNFTKGAIANLLNAFAKYTIVSFDKLDLKADFSQPIQINYSSNSYL
                     RKYADEQSFQIVKDILKSQE"
     gene            complement(6580..7134)
                     /gene="yqeK"
                     /locus_tag="PLU28_01305"
     CDS             complement(6580..7134)
                     /gene="yqeK"
                     /locus_tag="PLU28_01305"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR00488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HOG33733.1"
                     /translation="MEKLIKSKKRYEHIMEVEKYGLKMAEIWGADKNEVSVACLAHDL
                     FRDLPQNKIFLMCRAYSVSYDKYEEHNPNVLHGRLAAVFLKKKFNINKSIFEAVYYHT
                     SGYYKFDTLGKILFVSDALEAGRDYKGITKLRKASFTDLDLCYFLVLKSSLKYALKDN
                     LMLLKDTVKAWNHIAMEVQESGKD"
     gene            complement(7186..7467)
                     /locus_tag="PLU28_01310"
     CDS             complement(7186..7467)
                     /locus_tag="PLU28_01310"
                     /inference="COORDINATES: protein motif:HMM:NF016351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HOG33734.1"
                     /translation="MHCLKCEIILRCTGVNSLKEKRSILKKIIFRIKENYNVAIIESD
                     CNDSREFITIGVSALSDSKDFLYDLSKKIEEYIEDTFQILVVSEDVEIF"
     gene            complement(7468..7989)
                     /locus_tag="PLU28_01315"
     CDS             complement(7468..7989)
                     /locus_tag="PLU28_01315"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOG33735.1"
                     /translation="MSISTKTGDSGQTSLWSGERVFKDDIRVESYGTVDELNSFLGEA
                     MYYANSSEVKDILLSIQRDLFRVAGQLASKDVLYVKPVCDEDVQRLTDIVHHYEEIIK
                     LKGFVIPGSILASAKLDICRTVCRRAERRIVALNRVETVPVQVGQYINRLSDLLFIVA
                     RYEEYLQDKIIYK"
     gene            8151..8762
                     /locus_tag="PLU28_01320"
     CDS             8151..8762
                     /locus_tag="PLU28_01320"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOG33736.1"
                     /translation="MNNLVLAFPLYNYLKFCNESHNEKTILDCGAGGSNPPLSLFYEH
                     GYKTYGIEICKKQLEKANVFCKEQNMNLNIIWGDMLKIPFPNSFFGFLFSYNTSVHIK
                     KNDFMIALSEFHRVLKDDGLCYVNFLSENCDSCGEGKEISDGEYVTIQNNEEVLYVHY
                     KDTEIESLLSKFEIIYKEERTVKRKQNAKYISSGFYEYILKKK"
     gene            complement(8854..10926)
                     /locus_tag="PLU28_01325"
     CDS             complement(8854..10926)
                     /locus_tag="PLU28_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOG33737.1"
                     /translation="MGKYLPAQKRIVGVFGHHNSGKTTLLDAVLKNYYSGADRIGQRY
                     LDSEPIEKEKAASFSNHITTVDFEDTRLYFIDTPGSTEFLGDLQTAINASDNILLIVN
                     GTAGVEVSTERIWQMAREQNKPVIVFVAQMDKDGADYDKVISDLKEAFQDGVKLTPVQ
                     VPIGQGNNFKGITNVLLKKSFTYDVNTGKASEMSSLAPESQAWYDKYHDQAIEDIVEN
                     DEEILNKYFEGGEEAITTEELIKCLKKAVGEGTVVPVLIGSALHNAGIDRLMFSLKNY
                     GISPEHSKYSNMNDETIENSDKFTGLIIKNAVDPFVGKLTYLRILSGTLKASDSFYVV
                     QEDSNEKFSHLYIPRFDKNEEIEEASLGDIVVIPKLKNSKIGQTVVSAGQNVIVKVPD
                     FPEPMISKSISPKSKNEIDKITGALSKLSESDPTFKWEFDPETSETVISGIGTIHLEV
                     MTERLRKNFGVDFEIGKPKIAYRETIKRKTNAEYKHKKQTGGHGQYGHVKIEVEPLER
                     GSGYVFEDKIVGGVIPKNYIPSVDKGILEAIKKGILAAFPVVDIKVILYDGSYHDVDS
                     SDIAFQIAARSAFKIALETANPVLLEPIMKVDIFVPSANTGDAMGEVTAKRGRPLGME
                     NVGRGLDKIMAEIPLAEMLDFSPRLSSITSGKGYFSMKFEKYMEVTPEIQKKIVAERQ
                     AEMAAEQK"
     gene            complement(10970..12634)
                     /locus_tag="PLU28_01330"
     CDS             complement(10970..12634)
                     /locus_tag="PLU28_01330"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HOG33738.1"
                     /translation="MHKKIIFLVLIYIFAITAFSGDAVFISTDKEPSTAPLKVLGSEY
                     FIRVDDLVLEGLTVHKTENAIFMVFQKDILEINKQDQYAKLNFLTKYKSSIVMENGST
                     YLKADLLAKFLKRSYISADSNMFFYSQLPKIVNMTYSLNKLTVETDSIADEKCISFTS
                     SGEQKIIKLIPFQNSGIVPKEIDLTSSGGMITLKIKSTLNYKAVIQGKKIIIETVSSS
                     SSDKSVDKPNDPVVVLSGYQQKIVTIGEQKVKCYYAKYDPKKYKIQPVVNNLGISSKF
                     INVLSAQKPILAVNASYFDVSTLEPIGKIILNGRVLHIDSHLRPSFYIDKNGNPNMDY
                     IKLEYTAYLDDIPLLVKGVNSLWKANIKVYTSEYKNSIKEGEDEYLFYLIENGKVIST
                     AKKSPSGNELLLLVKRDYEKYLTEIVIDSVFRYETNNDKDINVYHMVDGGPFLINTEF
                     SKERLAEEKRGFSSIINGRTSRTLVAIDNDNMVTFILVEGNQKDYPGLDYDGCIELLN
                     QIGTFKKAMMFDGGSSCLFYADNKIINYREADWRESIPVMLGVFMK"
     gene            complement(12649..13959)
                     /locus_tag="PLU28_01335"
     CDS             complement(12649..13959)
                     /locus_tag="PLU28_01335"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HOG33739.1"
                     /translation="MRPYDIIQKKRDGFENTTQEIEYMIEGFVKSTVPDYQMAAWLMS
                     IYFNHMTAKERYDLTMAMLESGDKIDLSAIEGVKIDKHSTGGVGDKTTICLAPMVASM
                     GLKVSKLSGRGLGHTGGTVDKLEAIPGFRSSLSEKEFVDIANKYAVVVAGQTANIAPA
                     DKKIYALRDTTATVDEVSLISASIMSKKLAVMSDGIVLDVKTGSGAFMKSVSESVELA
                     KAMVDIAKLNQKKVVAVVTNMNQPLGETVGNSIEVLEAIQTMKGDGPKDLTELCFELA
                     ARMIQISGIDTYQNSKIILEKNIRSGKVAEIAKKWIQAQGGESKVIDDPKSFLNISSD
                     ILEFKAKKDGYIFKIDTQAVGVASMVLGAGRAKKEDIIDPSVGIKIVKKLGDYVKKGE
                     VIAYVYKSAASNHEESMKLLEHAYIIDKEKPDSDEYKLIYEVIE"
     gene            complement(13946..15223)
                     /locus_tag="PLU28_01340"
     CDS             complement(13946..15223)
                     /locus_tag="PLU28_01340"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG33740.1"
                     /translation="MLYMTLAILLTFFAGIMLAVVYFRQMKAIDSYKKLKEELCRILD
                     ININNPSDDFLLHQTNLKYKSLAERYESERVRRKNLYSIMNNLEEGVIVVSIYQSDII
                     KVDFANDFSKRIFTIDDYVGRSIVEIVDNHSLIDLILKSYKLQSDEEEELLFYSPEKR
                     FYKCKVKMMSIDDNGRIVLLSDITKEKNLENLRKEFLTTMSHELRTPLSVMNGYLETI
                     LMDNHLDKGLEKMLKIIEDETARLTRLVNDLLDLGRLEKSLTDNTNIKEINISNITKK
                     VFEFFKLLSEGLDVSLTAEVANNLKIKGDEERIMQAVYNIVDNALKFTSMKEKGEKNV
                     YVRLYKESSNVVLEIEDTGIGIPSKELEKIFDMFYRVDKSRTRQVSGFGLGLYIVKSI
                     LDKHNAGVYLESEENNGTLFRITFKGGIKDETL"
     gene            complement(15296..16024)
                     /locus_tag="PLU28_01345"
     CDS             complement(15296..16024)
                     /locus_tag="PLU28_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOG33741.1"
                     /translation="MAKKTIMIVEDDPAISEMLSINLSKEGYDVLTAGSADEALKVIE
                     EKDVDFFIVDIMLPGSMDGFDLIRILKSSETHKLTPMLILSAKDDSADKVAGLELGSD
                     DYVTKPFNVRELLARIKSIFRRQTSASQMKEEGPKKIIAKDLIIDTERLEVWVRGELV
                     GLTPLEFDLLVFLAKNEGKVFNRDVLLDKLWGYDYYGDTRTVDVHIRRLRTKIEEDPS
                     NPKYIITVRGKGYKFRDPGKEKND"
     gene            complement(16052..16486)
                     /locus_tag="PLU28_01350"
     CDS             complement(16052..16486)
                     /locus_tag="PLU28_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG33742.1"
                     /translation="MREVIEELKKLEGKEQVFNFKVLDDTFLWSEDEELLSLGTLSTS
                     ALLENVHRCGYSILKSVIPSNYVSVVGSTSIKHLSVTPVHSIVSIKILVKAVNENRVT
                     FEGEAFDEFEKVASFSFERVVVSLGSVSRKIKEKSDKIKSIN"
     gene            complement(16470..18356)
                     /gene="ftsH"
                     /locus_tag="PLU28_01355"
     CDS             complement(16470..18356)
                     /gene="ftsH"
                     /locus_tag="PLU28_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOG33743.1"
                     /translation="MADNRKPQKTIGVLFVYIVLALLIYFGISGLTNTQSVKDISYSE
                     MISFIESKQVMNIDIEDTGMMSLKLIDGTKYNVYNPGMMIDQDFIYALSRDGIKIRFV
                     KGISSSWWINIITYLIPIIFLVFMWFLMFRPMSRGPQGMNFSKSPARKYDPEKDKITF
                     NEVAGVDEAKEELSDVVQFLKDPKSFVKIGARMPKGIILVGNPGTGKTLLARAVAGEA
                     GVPFFFISGSDFVELFVGVGAARVRDLFTQAKASAPAIIFIDEIDAVGRQRGAGLGGG
                     HDEREQTLNQLLVEMDGFDSKTGIVLMAATNRPDILDKALLRPGRFDKKVVIDSPDLN
                     GREAIVKIHMRGKPIAQDVDPNVLARRTPGFVGADLENLVNEAALLSARKKKNVINME
                     EFEEAIDRVIAGPSRKSRVVSEKEKKIIAYHELGHAIIGSLLPNADPVHKVTIVPRGH
                     QALGYTLQLPLEDKYLLSRSEMLDRIVAILGGRAAEEVIFGEVTTGAGNDLKKATEYA
                     RLMVCQLGMSDKIGPIAWGEEEEEVFLGREITKMKNYSDETAALIDSEMNKIITDGYE
                     KAKELVRQNIQKFDFIARYLLSKETILGDELKKMLEFDIGELEKYIGSMELKEKVVKS
                     EGSN"
     gene            complement(18360..19319)
                     /gene="tilS"
                     /locus_tag="PLU28_01360"
     CDS             complement(18360..19319)
                     /gene="tilS"
                     /locus_tag="PLU28_01360"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOG33744.1"
                     /translation="MTIKLSEIERKIFQFIKDYEIFKKCDKILIGVSGGKDSMTLLNI
                     MYKLSKLSGYQLGVAHFNHKLRKESDKEQEFVRSYCEGLSVPFFTAEGDVIGYCSKNK
                     KTIETGARELRYEFLYEILRKYGYNKIATAHHASDLAETVIYRLSRGTGIIGMGGLSP
                     VNGKVTHPMLTVTLKEILDYVTINNVKHVNDSSNEDKKYTRNAIRLDILPVLENINPK
                     YDENILRFCQIVWSYKEQVEKIYDERVSETGEVYEFKLRYDFFDSEVLRMIFLKNGKY
                     PPNYEESCKIISMKNGGRRKFGNIVIKRKKDSLTLQVMKQREA"
BASE COUNT         5723 a         4121 c         2875 g         6728 t
ORIGIN      
        1 ccgcctcctt ttatatcaag atcccatggc ttgcctatac caagatgctc cataagttca
       61 tgcggcatgt attgagtaac aactccaact ttggttattc cggagtttac acagttgctt
      121 actgtgaaat ctatcatcct gtactttcct gcaaaaggaa cagccggttt ggcaatttca
      181 ttggttatta ccccgagtct ggttccctga ccgcctgcca gaattaagga gacaacattc
      241 atcatcttcc ccccctttta tataaccgaa tctttttcaa tggttagtat ctcacctata
      301 ccttcaatac gtttggcatt acgcaacgtt acacttttgt caagaatagc gttttttagt
      361 atggcacctt cttctattac tgaatcttcc attattatag aattttctac acttgcacct
      421 gcttttatta ttactcctct ggaaattatg gaatttttga cctgtccgtt gactatgcag
      481 ccatcggcta taagtgagtt gcttacacaa gcatttatat ttatcttagg aggagcataa
      541 tctttaagtt tggtatatat cttccttcct gaatagaaaa gttctttcct gacctctctc
      601 ttgagtatat ccatattggt atcgtagtac tcttttatag actttttgat gtttctccag
      661 tatccagaat gtttgtatgc ataaacccta agcttggata tgtttggaat aataatgtcc
      721 ctgaccatat catagccatt attcggaacc gatgagtaca gaagttccag aagaagggct
      781 ttatttataa aataaacccc catagatata tcttcatata tgcaatcggt tggtttttct
      841 acaaattcgc taattcttcc gtcattatca acagctaact gaccgtaatc gcatatcctt
      901 gtatcctccc tacagatctt gcttgaaata actgtgatat cagcaccttt gcggatatgg
      961 tacttaaaaa gatccatata actcatatta tagatgtggt ctcctgagcc tatgaggaca
     1021 aagtcctcgt cgcctctcct caaaaaggat atattctgaa agatagcatc cgcagttcct
     1081 ttatagtata tgctctcacc gctacctacc ataaatggct gaaggataaa tatcccgcct
     1141 ctctttctgt caaggttcca ttcctttccg ccggcaagat gatccataag gcttctggga
     1201 ttgtactgcg taagtactcc aaccttcgaa ataccggagt ttaccatatt acttaaagta
     1261 aaatctattg ccctgtactt tccaaacatg ggaacagctg cactggttct tattctggtc
     1321 agtggtttca aattatcctg atttgaaccg cataaaatca aacccagtac tctcatagca
     1381 tttccccctt attttatttt aagccacaca cattttaagt aaaaactctc tggaatctgt
     1441 attatatagg gatgatcctt tgactgaaaa gtgaatccaa ccatctggat actcctgccc
     1501 gtaccttcaa atgccattct tgaagcttct atgagcatat cagtgtcagc ttggtatgca
     1561 caactgcaaa gacagactat tccgttggtc tttattttag gcacaatcaa tttaacaatg
     1621 cctttaaata tgtcaaggcc tttccttctt tcttccttct tttttattaa agacggaggg
     1681 tcaagaatta ctacatcaaa atcttctcct gaatactcct gcataaactt ttcataagtt
     1741 ccggagaaaa actctatatt tccaagatta ttaatatcca catttttttt agccatttca
     1801 agcgactgtt cgtctttatc aaccgcatat actttttttg cacccgaaac agccatattc
     1861 attgcaaatc cgccggtgaa agtatacgca tcaagacata ccgaactagt gtctacaaac
     1921 tgtctcacaa aaaaccttga atccttctga tcgaagaaaa atcctgtttt ctgaccgttt
     1981 attatatcca cattatactt cagtccatgt tcgtgaacaa taagtgtatg ctcaggttct
     2041 ttaccgaaaa caactccttt gtttcttttt atattttctg cagaaccggt ttcaaaatca
     2101 cttctttcgt atattccgac aggcgcatat acctttgcaa aagcctcaat tatctgggcc
     2161 tttttatttt ccacgccaac atttcttaac tgaatcacta tatagtcatt atatttatcg
     2221 gctataagac caggtactcc atcagcttcc ccatgaaata tcctacaaga atctaaatta
     2281 ttcatgattg cttttcttaa ttcaaaagcc tttaaaactc tactgtagaa aaattcataa
     2341 tcaatttcat catcattaag aaccagcatt ctgaccgcaa tattggaaga agaaaaaaaa
     2401 cctttcccca agaacttcat actatctgaa aaaacagtgc aaatctctcc gttcttttta
     2461 tctccgacaa cagaaaaagt ctcatcggaa aaaatccacg gatatccatt gagaacttta
     2521 cgttctttcc ctctttttaa agtaactaca gtcatctggt tcctccatta aatttctttt
     2581 tcataaactg cttcatattc aaatagctca ttttcattga aaaagatctt catctccctt
     2641 aaagcatttt cgtgcgaatc agatgagtga acaacatttt tccgcacaga aagaccatac
     2701 ttacctctta tgcttgccgc ttcagcctta agaggatctg ttgaaccaca gatatgcctt
     2761 atatgctgaa cagctctttc tgcagacacc accaatgcaa ccactggacc tgagatagca
     2821 taacttacaa gcggttcaaa gtaatccctt ccctcatgtt ctttatataa ttcacaagcc
     2881 tgtgccctgg aaatccaaag catcttcata gcaataattt taatgcctct tttttccact
     2941 tcacttataa tactgcctat aagccctctt tttactgtag acggtttgat gatcaaaaat
     3001 tctttctcca tttttccacc tcacagtatt atgccgtcag acatgtttag gactctgtct
     3061 gcatattttt ttgatacttc atcatgtgaa acaacaaaaa aagatacata ctctttgagc
     3121 tggaaaaaaa gttcaaagac atttcttttg ttttcagaat caagcgaacc agttggttca
     3181 tcacaaaaaa ccagttcaac aggtccggag attgatctga gtacggccga cctctgaagc
     3241 tgtcctactg aaagttcagc tggatagcgg tagaggatat cggttatacc aattttttct
     3301 gcatagtcaa aaatttgccg gtcgtcgtca aaagacctta tattctgagc aactttacag
     3361 ttctcaaaaa cagttaattc attgagcata ttataatttt gaaaaacaaa cccaatcttt
     3421 cttttcttgt cctctatatt atataatatt tttccagcgt cttgggtcag aagtccgcct
     3481 aatatatata taagtgtaga ctttccgctt ccggatggtc catatagagt gagtatttca
     3541 ttttttaata cttttatgtc tactcctttt aaaacaggat tttttttatc atacgagtaa
     3601 aaaatacctt cggcagttaa aataattttc tcattcatcc tttaaagcct ccaaagtatc
     3661 tatacctgag atcatacggg tagttaagaa gctgaaaaaa actacaatac cagtgattat
     3721 aaaaaatgaa ctccctgcta ttgaaagatt tagagataca ggaagataat ctacataaaa
     3781 tatccctgaa ggaatcttta ttttaaaaaa atccagcata gtaaccgtta acatacctcc
     3841 cgcaagtccc aaaagatatc caataaccga aataataagg ctttcagaaa taaaaacttt
     3901 ttgaatatcg atgtttttca ttcccaatga cttgaggatg ccgatttcct ttttccgtgt
     3961 aagtatgctg aaggtaatag agtttgaaac tgaaaaacct gccataagaa ctataaaaac
     4021 ggttataaga agggcaataa gcccatctat ttccactgtc tgtgtaagcg ttttatttga
     4081 ttccttccat gtatatgccg gactatatga aaagtactta tttttatact catcagcttt
     4141 tagaggatct tcaaagtaat aagctgtaaa atttttcacg gaatccggat catttataac
     4201 caaaaatttt gaatcaaaaa tataaactcc tgttttaaac aaagatacaa ccctatactt
     4261 tttagctata ggaatgatgc ctttaatctt aaaaactgtc agttcatctc ctatggaaat
     4321 atttaaatat tccgaaagct ctttgccgat agcagctccg ttttccagtt gtccggaaat
     4381 tatacttgac ccaagaaatg acatgtcatc gcagtccatg agctgcacat aatcaaatga
     4441 tccattaaaa gcgaccatag agttatcaag gttaaaaaac atcttttctt ttactccgga
     4501 aaaataaaga tcactttttc cagcaacagt aatgtgtggg taaaatctgg ttatcgaatc
     4561 gataagaagt ctgtcaaatc cattgaccac agaggtaata acgataagac cccacactcc
     4621 tatggcaatg gctattgtcg gaaatttaaa gctcttacga gattttttta tgaatccctt
     4681 tactagtgaa aatactgctt tatctgtaaa tctcttattt gtcacctata atcaccactg
     4741 catcgtataa tgtaaaatca ataaagcttg aggatacctt tttaactata gaaaaatatg
     4801 tctctcctgc cgttggtata tcgataaaat ccttgctgaa ggtagcctgc gtgtcttttt
     4861 ttatattggg atacttctgt tggtttttca cgagtatata gctttttgat ttttccaaaa
     4921 aatcaaaggt tacatcaggc acatatataa gatatccctt agctttccat tttgcatata
     4981 tttcattata aaaagcaata taatacgctg aattatttcg tataaacata actgaaggtt
     5041 tttttacttc ctcgtttttt tcaaacttaa caagatatgc ttttacttct tccagtcttt
     5101 tgttatctga tataacataa ctttccttta ttatataagg aaattcaaga aactctattt
     5161 cggaactttt ggcataagag taaagtttga aaagatcaga tgatgaaaat gtatttatag
     5221 tttctttttt tacaatatct atcagatcaa ccagtttggt cattcctaga tttttggctc
     5281 tatcaaggat agagtaaaat gcctttttct gtctttgtat ccttcctaaa tcgcccatct
     5341 catcgtttct gaatctcatg taatagagaa gtttttcgcc gtccatatcg ttatatccct
     5401 gcttaaagtt tataaaaaga ttctgatgat agtctgtata ggacatattg ctctctacaa
     5461 aaacacgaac gggggcaatt tcatccccca gtgtcttaaa tattgaataa ttgaatatta
     5521 tatagtcttt cacctgaact ccagaaagat ccgaaactgt tttaaggaag ctttctttgc
     5581 ccagagtcga gtacagggca tttatcctct tttctgtgtt tcccgaactt atcataagat
     5641 ctctcggaat agggagaaaa attatctttc ctttatttat accagcaagt attatagtat
     5701 cggttctgct tacagactgg gacgtatcac tatccattcc gattatgaga aaataataag
     5761 gaagtgaaat atcgcttttg aatacaatag ctttcttcac agaaaaaaga accgttgtaa
     5821 gtataagcac aagaaaaagg cttccgaaaa cactcaaata tttcctcaaa agtttttcac
     5881 tcctgacttt ttagtatatc tttcacaatc tgaaagcttt gctcatcagc atactttcta
     5941 agataagagt ttgaactgta gttaatctgt attggctgtg aaaaatcagc tttaagatcc
     6001 agcttatcaa aactaactat agtatatttt gcaaaagcat tcagaagatt tgcaattgct
     6061 cctttggtaa agttactatc cctatcatac tttcttataa gaccttccag actgtttaca
     6121 aaaaagaaat cgaaaatatt actgttcctg tacttcaacg cttcaaaaaa agattccata
     6181 tcttctttct gactctttac agattgatta agttttttta cggtattctc atcgactttt
     6241 atataaaaat ctagatttga gctgagttca aattctttca gaaagctgtt tgtcgaatcc
     6301 ttaagattat cctttgaaat gctgaaaagc ttatctttga atgtaagtcc atcttctgcc
     6361 cttactataa gaattttgtt cctgtcatta agaactctca gaaaataaat cttatcccca
     6421 taagaaaatg tatacgttga ttttgccagc ttcaattccg acgaagagtt cagagcatag
     6481 atattatata cagcaaatgc tccaaccaac aaaactgcta taaccactac aaaaattacc
     6541 gttttaagaa aacttccctt tttccttcct ctttttactt taatccttgc cactttcctg
     6601 cacctccata gctatatgat tccaggcttt aactgtatct ttcaataaca taagattatc
     6661 ttttaacgca tacttcagac tgcttttaag aacaagaaaa tagcataagt ctagatctgt
     6721 aaatgaagcc ttacgaagct ttgttatgcc cttgtaatct ctgcctgcct caagagcatc
     6781 tgaaacaaaa agaatctttc caagagtatc aaacttatag tatccactgg tatgatagta
     6841 aacagcctca aaaatacttt tgtttatatt aaattttttt ttaaggaata cggctgccaa
     6901 ccttccatga agaacattag gattatgttc ttcatacttg tcgtaactta cagagtaggc
     6961 tctgcacatc aaaaatattt tattctgggg taaatccctg aaaagatcat gtgccagaca
     7021 agctacactg acttcattct tatctgctcc ccagatttct gccattttca acccgtattt
     7081 ttcaacttcc attatatgct cataccgttt cttacttttg ataagctttt ccaaacaatc
     7141 atttaaagta tcttttatgt ataaaaaatt cagaatattc ctcctttaga aaatctctac
     7201 atcctcagaa actacaagta tctgaaaagt atcttctata tactcttcaa tctttttgga
     7261 aagatcgtat aaaaaatctt tggaatcact aagagctgaa acccctatag taatgaactc
     7321 ccttgagtca ttacagtcac tttctattat tgcaacgtta taattttcct ttatgcgaaa
     7381 aattattttt tttaagatgc ttcttttctc ctttaatgag ttaactcctg tacatcttag
     7441 aattatttca catttaagac aatgcatcta tttatagatt attttatcct gaagatactc
     7501 ttcatatcgt gcaactataa acaaaagatc cgataatctg tttatatact gcccaacctg
     7561 aaccggaaca gtttcgaccc tgtttaatgc tactatgcgc cgttctgccc tgcgacagac
     7621 cgtcctgcag atatccagct tggccgatgc aagaatagaa cccggtataa caaaaccttt
     7681 aagcttgatt atttcttcat aatgatggac aatatccgtc agtctctgaa catcctcgtc
     7741 acaaaccggt ttaacatata gaacatcctt tgaggcaagc tgtccggcaa ctctgaaaag
     7801 atccctctga atgctaagca gtatatcttt aacttcagac gaattggcat aatacattgc
     7861 ctctcccaaa aaagagttca gttcgtcaac cgttccatac gattctactc ttatatcatc
     7921 tttaaaaact ctttctccgg accataagct tgtctgtccg gaatcgccag tcttagtgct
     7981 tatgctcatt ctatcacccc ttactattat accttaagag gtttatattt caaagcagca
     8041 tttctttttt ttaaagtcca tgccataaag ctttcaatgt ttttgctgcg atgatatttt
     8101 tatagtataa ttaattcaca taaaaatatc ttatctctta aaggtggatt atgaataatc
     8161 ttgtattagc ttttccttta tataattact taaaattttg caatgaaagt cataatgaaa
     8221 aaactatcct tgattgtggt gcgggaggtt ctaatcctcc tctttcccta ttctacgagc
     8281 atggctataa aacttacgga attgaaattt gcaaaaaaca gttagaaaag gccaatgttt
     8341 tttgtaaaga acaaaatatg aacctaaata ttatttgggg agatatgtta aagatacctt
     8401 ttcctaatag tttttttggt tttttatttt cttataacac ctctgtccat attaaaaaaa
     8461 acgattttat gattgcttta tccgaatttc atagagttct aaaagatgat ggattatgtt
     8521 atgtgaattt tttgagtgag aattgcgatt cttgtggtga aggtaaagag ataagcgatg
     8581 gtgagtacgt cactatacaa aataatgaag aagtacttta tgttcattac aaagataccg
     8641 agattgagag ccttcttagc aagtttgaaa tcatttataa agaagaacgt acagtcaaaa
     8701 gaaaacaaaa tgctaaatac atttcttccg gtttttatga atatatcctg aaaaaaaagt
     8761 agttgtataa aaattaattt cacgctttat gaaatataat tttttatagt ttttgtaaaa
     8821 aaaaacggag accagaggtc tccgttttgt tatttatttc tgttcggcag ccatttcggc
     8881 ttgtctttcg gcaacaattt ttttctgtat ttcaggtgtt acttccatgt atttttcgaa
     8941 tttcatggaa aaatatcctt taccgctggt tattgaactt agcctaggtg aaaaatccag
     9001 catctcggca agaggtattt cagccattat cttatccaat cctcttccaa cattttccat
     9061 tccaagtggt ctgcctctct tagcagttac ttctcccatt gcatctcctg tattggcact
     9121 aggaacaaat atatctacct tcattatagg ttccagaaga acaggatttg cagtttcaag
     9181 ggctatttta aaggcgcttc tggcagcaat ctgaaatgca atatcagacg agtctacgtc
     9241 atgataagag ccatcgtaga ggattacctt tatatcaaca acaggaaacg ctgcaagtat
     9301 tcctttcttt atagcctcca gtattccttt gtcaacggaa ggtatatagt ttttcggtat
     9361 gactcctccg acaatcttat cctcaaatac ataacctgaa cctctttcta gtggttctac
     9421 ctctatcttg acatgaccgt actgtccatg tccgccagtc tgctttttat gtttatattc
     9481 cgcattggtt tttcttttta ttgtttctct ataagctatc ttgggctttc ctatttcaaa
     9541 atccactcca aagttctttc ttagtctttc tgtcattact tcaagatgta tcgtacctat
     9601 accgcttata actgtctcac tggtttcagg atcaaattcc catttaaaag tggggtcaga
     9661 ctctgaaagc tttgaaagtg cgcctgtaat cttatctatt tcatttttgg atttgggcga
     9721 tattgatttt gatatcatag gttcaggaaa atcaggaact tttacaataa cgttctgtcc
     9781 tgctgatact accgtctgtc caattttact gttcttaagc tttggtatta ccactatatc
     9841 ccccagcgag gcttcttcta tttcttcgtt cttgtcaaat ctaggaatat aaagatgcga
     9901 gaacttttca tttgaatcct cctgaactac atagaacgaa tcggaagctt ttagagtacc
     9961 gctgagtatc ctcaaataag taagcttacc tacaaaagga tcaacagcat tcttgattat
    10021 taatcctgta aacttatctg aattttcaat cgtttcatcg ttcatattag agtactttga
    10081 atgctctggc gatatgccat agtttttaag ggagaacata agtctgtcta ttcctgcatt
    10141 atgcaaggct gagcctataa gcaccggaac aacagttcct tcaccgacag cctttttcag
    10201 acatttaata agttcctctg ttgtgattgc ctcttctcca ccttcaaaat acttatttaa
    10261 aatttcctca tcattttcca caatatcttc gatggcctga tcatgatact tatcatacca
    10321 tgcttggctt tcaggtgcca aagaggacat ctccgaagct tttcccgtgt taacatcata
    10381 tgtgaaggat tttttaagca gtacatttgt tattccttta aagttatttc cctgtcctat
    10441 tggaacctga actggagtta acttcacacc atcctggaaa gcttctttca gatctgaaat
    10501 aactttatcg taatccgctc cgtctttatc catctgagct acgaatacaa taacgggttt
    10561 attctgttct cttgccatct gccatattct ttcagtagat acttcaactc cggcagttcc
    10621 attcactatc aataaaatat tatctgaagc atttattgca gtttgaagat ctcctaaaaa
    10681 ttctgttgaa cccggagtat ctataaaata caatcttgta tcttcaaaat caacagtcgt
    10741 aatatgattg gaaaaacttg cggctttttc tttttcaatg ggctccgaat cgagatatct
    10801 ctgtccgatt ctgtccgctc ccgaataata atttttaagt actgcatcaa gaagtgtagt
    10861 ctttccgcta ttatgatgtc caaatactcc tactatcctt ttttgagctg gtaaatattt
    10921 ccccatataa agtgccacct ccgtagattt tggtagataa ccatgagcgt tatttcataa
    10981 agacacccaa cattacaggt atcgattcac gccagtctgc ttctctataa ttaataatct
    11041 tattatctgc atagaaaaga caagaacttc cgccatcaaa catcatggct tttttgaatg
    11101 tacctatctg attcaacagt tctatacatc catcatagtc aagaccgggg taatcttttt
    11161 ggttaccttc aaccaatata aatgttacca tattatcatt atcaattgca acaagcgttc
    11221 ttgacgttct accgttaata atactgctga agcctctttt ttcttccgct agcctttctt
    11281 ttgaaaactc cgtgttaatt aagaaaggcc ctccgtctac catatgatag acatttatat
    11341 ctttatcgtt attggtttca taacggaaaa cagaatctat tacgatctct gtaagatact
    11401 tttcataatc tctttttaca agtaaaagca gctcgttacc ggaaggactt tttttcgctg
    11461 tggatatcac tttaccgttt tcaataagat agaatagata ctcatcttcg ccttctttta
    11521 tggagttttt gtactctgat gtatatactt ttatattggc tttccaaaga gagtttacac
    11581 cctttaccaa aagcggtata tcatcaagat atgctgtata ctcaagtttt atataatcca
    11641 tgttcggatt gccgttttta tcaatataaa acgaaggtct caaatgtgaa tctatatgaa
    11701 gaacacgtcc gttaagtatt atcttaccta tcggttcaag tgtggaaaca tcaaagtaag
    11761 aagcattaac agccagaata ggtttttgtg cagacagaac atttataaat tttgaagaaa
    11821 tgccaagatt gtttactact ggctggattt tatacttctt aggatcgtac tttgcataat
    11881 agcattttac cttctgctca cctatagtaa ctatcttctg ctggtatcct gaaagaacaa
    11941 ccaccgggtc attcggttta tcaacagact tatcgctact gctagatgaa actgtttcaa
    12001 ttattatttt ttttccttgt attacagctt tgtagttcaa ggtacttttg atctttaaag
    12061 ttatcatacc gcccgagctg gtaagatcta tctccttggg aactattcct gagttctgaa
    12121 aaggaataag cttgataatt ttctgttccc ccgatgacgt gaaagaaata catttttcat
    12181 cagctataga atctgtttct accgttaatt tatttaaaga ataagtcata ttgactatct
    12241 tgggaagttg tgaataaaaa aacatattgc tgtcggcact tatatacgat ctttttagaa
    12301 attttgccaa cagatctgcc ttaagataag tacttccgtt ctccataaca atagaactct
    12361 tgtatttggt aagaaaatta agcttggcat actgatcctg cttatttatt tcaagaatat
    12421 ctttttgaaa aaccataaaa atagcgtttt ccgttttatg cactgttaat ccttccagaa
    12481 caagatcatc aaccctgata aagtactctg atcccagaac ctttaacgga gcagttgatg
    12541 gctctttgtc agtactaata aaaacggcat cacctgaaaa tgccgtaatg gcgaaaatat
    12601 atataagaac aagaaaaata atttttttat gcatcagttc tcacctaatt actctattac
    12661 ttcatatatg agtttatact catctgaatc tggtttttct ttatctatga tataggcatg
    12721 ctcaagaagc ttcatcgact cttcatggtt acttgcggca ctcttatata catatgctat
    12781 aacctcacct ttttttacat aatcacccag cttttttaca atttttattc ctacagaagg
    12841 atctataatg tcttcttttt ttgctctgcc ggctccaaga accatcgatg ccacacctac
    12901 cgcctgggta tctattttaa aaatgtatcc atctttcttc gctttaaact ctaggatgtc
    12961 tgaagaaatg ttcaaaaaac tcttcgggtc atctattacc tttgactcgc ctccttgagc
    13021 ctgtatccat tttttagcaa tttccgcaac ctttccggac cttatatttt tttccagtat
    13081 aattttggaa ttctggtaag tatcaattcc agatatctga atcatccggg cagcaagttc
    13141 aaaacacaac tcggtaagat ccttaggtcc atcacccttc atagtctgta tagcctcgag
    13201 aacctctata gaatttccta ctgtttcacc aagcggctga ttcatgtttg taaccacagc
    13261 aaccactttt ttttgattaa gctttgcaat atcaaccatg gcctttgcaa gctcgactga
    13321 ttcactgaca cttttcataa aagctcccga gccggtcttt acatccaata caattccatc
    13381 ggacatgaca gccagttttt tgctcattat actcgctgat ataagggata cctcgtcaac
    13441 agttgccgta gtatctctaa gtgcatatat ttttttgtct gcaggagcaa tgttggccgt
    13501 ctgccctgct acaacaacag catatttatt agcaatatct acgaactctt tttcgctcaa
    13561 tgaacttctg aaacccggta ttgcttccag cttatctaca gtaccacctg tatgacccaa
    13621 tcctctgccg gaaagcttcg aaaccttcaa tcccatagaa gctaccatag gggcaagaca
    13681 tatggttgtt ttatctccta ctccgccggt ggaatgctta tcaattttta caccttctat
    13741 ggcactaaga tctatcttat caccagattc aagcatggcc attgtcaggt catatctttc
    13801 tttagcggtc atatgattaa agtatattga cataagccaa gctgccatct gatagtcagg
    13861 tactgtagat tttacaaatc cttcaatcat atactcaatt tcctgggtag tattctcaaa
    13921 tccgtctctc ttcttctgaa tgatatcata gggtctcatc tttaatacct cccttgaaag
    13981 taatccggaa tagcgtaccg ttattttcct cactttcaag atacactccg gcgttatgtt
    14041 tgtccagtat cgatttaact atgtataatc ctaatccaaa accacttacc tgcctagttc
    14101 tagacttatc aacacgatag aacatatcga atattttttc aagctccttt gacggtattc
    14161 ctattcctgt gtcttctatt tcaagaacta cattactgct ttctttgtaa agtctcacat
    14221 aaacattttt ttctcccttt tccttcatag atgtaaactt gagggcgttg tccacaatat
    14281 tataaaccgc ctgcattatt ctttcttcgt caccttttat tttaaggtta ttggctactt
    14341 cggcggtaag acttacatcc aagccttcag aaaggagctt aaaaaattca aagacttttt
    14401 tggttatatt ggaaatatta atttctttta tattagtgtt atcagtaagg gatttttcaa
    14461 gtcttcctag atcaagaagg tcatttacta gtctggtaag ccttgcagtc tcatcctcta
    14521 ttatttttaa catcttttct aaccctttgt caagatgatt atccataaga atggtttcaa
    14581 gataaccatt cataacagaa agaggtgttc tgagctcatg tgacatagta gtcagaaatt
    14641 cttttctgag gttttcaaga tttttctctt tggttatatc cgaaagcagt actatacgtc
    14701 cgttatcatc tatgctcatc atttttactt tacatttata aaaccttttt tccggtgaat
    14761 agaacaaaag ctcctcttct tcatcagact gaagtttata gcttttgagt atcaggtcta
    14821 tgagactgtg attgtctact atttctacta ttgatcttcc aacataatca tcaattgtaa
    14881 agattctttt tgaaaaatca tttgcaaagt caacctttat tatgtcggac tggtatatcg
    14941 aaacaactat tactccctct tcaagattat tcattatgga ataaaggttc ttccttctta
    15001 ctctttcact ttcatacctt tctgcaagac ttttatactt aagattagtc tgatgaagga
    15061 gaaagtcatc tgaaggattg tttatattaa tatcaagtat tctgcaaagc tcttctttaa
    15121 gttttttgta ggaatcgata gctttcatct gtcggaagta tacgacagcc agcattattc
    15181 ccgcaaaaaa agttaataat attgccaatg tcatatacag catattatta cctgcctttt
    15241 tttatattca aaaaacgaca gaggacaccc tctgtcgtca ataaccaata gttaatcagt
    15301 cgttcttttc tttgccggga tccctgaact tatatccttt tcctcttaca gttattatgt
    15361 acttaggatt ggaaggatct tcctcaatct tagttctgag tcttcttata tgtacgtcta
    15421 ccgttcttgt atcgccgtaa tagtcatatc cccaaagctt atccaacaga acatcacggt
    15481 tgaaaacctt tccttcattc ttggccagga agaccaaaag atcaaattca agcggagtca
    15541 atcctacaag ctcgcctctt acccatacct caagtctttc ggtatctatt ataagatctt
    15601 tggctattat ctttttcggc ccttcctctt tcatctgtga agcagatgtc tgtcttctga
    15661 aaatactctt tattcttgca agaagttctc tgacattaaa tggcttggtc acataatcat
    15721 cgcttccgag ctcaagacca gcaactttat ctgccgaatc atcttttgca ctgagaataa
    15781 gcataggagt aagcttatga gtttcactgc ttttaagaat tcttataagg tcaaacccat
    15841 ccattgatcc gggtaacata atgtcaacta taaaaaagtc aacatccttc tcctcaatta
    15901 ctttgagcgc ttcgtcagca cttccggctg taagaacatc gtatccttct tttgaaaggt
    15961 taatcgataa catttcggaa attgctggat catcttctac tatcataata gtcttttttg
    16021 ccatctgtaa ttcctccctt caattttatt actaattaat gctttttatt ttatctgatt
    16081 tttccttgat cttacggctt accgaaccca gagagaccac taccctttca aaactaaagg
    16141 aagctacctt ttcaaactcg tcgaatgcct ctccttcaaa agttacccta ttctcattta
    16201 ccgccttaac aagtatcttt atactgacaa tggaatgaac cggagttacc gagagatgct
    16261 ttatggatgt agacccaacc actgaaacgt aatttgaagg tataacactt tttaaaatac
    16321 tgtagccgca acggtgaaca ttctcgagaa gtgcagatgt gcttaacgtt cccagactaa
    16381 gaagttcctc atcctctgac cacaaaaaag tgtcatccag taccttaaag ttaaaaacct
    16441 gttccttccc ctcaagtttt tttagttctt caattacttc cctcactttt taccaccttc
    16501 tctttaagct ccattgaccc tatatacttt tccagctcgc ctatatcaaa ctcaagcatt
    16561 ttcttcaact cgtcgcctaa tatagtttct tttgaaagca gataccgggc tatgaagtca
    16621 aacttctgaa tattctgcct tacaagctct ttggcctttt catatccgtc ggtgattatt
    16681 ttgttcattt ctgaatcaat aagtgcggca gtttcgtctg aataattctt catcttagtt
    16741 atctctctgc ctaggaaaac ttcttcttct tcttcccccc aggcgatagg acctatttta
    16801 tcgctcatac caagctggca taccataagt cttgcatact cagtagcttt ctttaggtca
    16861 ttcccagcac ctgtcgttac ctcgccgaaa ataacctctt cagccgccct tccgcctaaa
    16921 atggcaacta tcctgtctag catttcagat ctggataaga ggtacttatc ttcaagcggg
    16981 agctgcaaag tatatcctaa tgcctgatga cctctgggta caatcgttac tttgtggaca
    17041 gggtcggcat tcggaagcaa acttcctatt atagcatgac cgagctcatg ataggctatt
    17101 attttctttt ccttttccga aacaaccctt gattttcttg aaggaccggc tattaccctg
    17161 tctattgctt cttcaaactc ttccatgttt ataacattct ttttctttct ggctgaaagc
    17221 aacgctgctt cattcaccag attttcaaga tccgcaccaa caaatccggg cgttcttcta
    17281 gcaaggacat ttggatcaac atcctgagct ataggtttgc ctctcatatg tatctttact
    17341 atagcttctc ttccgttcag atcgggagag tctataacaa cttttttatc gaatcttccc
    17401 ggtcggagaa gtgccttatc caggatatca ggcctatttg tagctgccat aagtactatc
    17461 cctgtttttg aatcaaaacc gtccatctcc acaagcaatt ggttaagagt ctgttctctc
    17521 tcgtcgtgac ctccgcctag acctgcacct ctctgacggc ctacggcatc aatttcatct
    17581 ataaatatta ttgccggggc acttgcttta gcctgcgtaa aaagatctct tacccttgca
    17641 gcacctactc ctacaaaaag ctcaacaaaa tcagaaccgc ttataaagaa aaacggaact
    17701 ccagcttctc ctgcaacagc tctagccaaa agagtttttc cggttccggg atttccaacc
    17761 agaattatgc ccttaggcat tcttgcacct atttttacaa aactcttggg atccttaagg
    17821 aactgaacca catcactcaa ctcttcttta gcctcatcga cgcccgcaac ttcgttgaaa
    17881 gtaattttat ccttttcggg atcgtactta cgcgctggac tctttgaaaa gttcattccc
    17941 tgaggtcctc tgctcatagg cctgaacatg agaaaccaca tgaaaacaag gaaaattatg
    18001 ggtataagat aagtgattat gtttatccac caactggaag atatcccttt gacaaacctg
    18061 atctttatac catcacggga caaagcatag atgaaatcct gatcaatcat cattccagga
    18121 ttataaacat tatatttggt accgtctata agctttaagg acatcatacc ggtatcctcg
    18181 atatcaatat tcattacctg tttgctttca atgaacgata tcatctcaga gtagcttata
    18241 tctttaacgc tctgtgtgtt ggttaatccg ctaatgccaa aataaattaa tagagctaaa
    18301 actatataca caaaaagcac acctatagtt ttttgcggtt ttctgttatc agccaagatt
    18361 catgcctccc tttgcttcat gacttgtagt gttagactgt ctttttttct ttttatgaca
    18421 atattaccaa attttcttct gccaccgttt ttcatgctta ttatcttgca gctttcttcg
    18481 tagttgggtg gatatttccc gtttttaaga aaaatcatcc gcagtacctc agaatcaaaa
    18541 aagtcatacc tgagtttgaa ctcataaact tcacctgttt cagaaaccct ttcatcataa
    18601 attttctcaa cctgttcttt ataagaccat acaatctgac agaaccttag aatattttca
    18661 tcgtacttgg ggtttatatt ttctaaaact ggcaatatat caagccttat agcatttctt
    18721 gtatattttt tgtcctcgtt tgaactgtca ttaacatgtt taacattatt aattgtaaca
    18781 taatccaata tctctttcaa agttacagtt aacataggat gtgtaacttt tccgttaaca
    18841 gggctcaaac ctcccattcc aattattcct gtacctcttg aaagcctgta aataacagtt
    18901 tctgcaagat cagaagcatg atgagcagta gctattttat tatagccata ttttcttaaa
    18961 atttcatata aaaattcata tctcagttca cgtgctccgg tttctattgt ttttttattt
    19021 tttgaacaat atccaatcac atcgccctct gctgtgaaaa aaggtacgga aagtccttca
    19081 caataacttc tgacaaactc ttgttccttg tcagattctt ttctgagctt atgattaaaa
    19141 tgggcaaccc caagttggta tccggataac tttgaaagct tgtacataat attcaaaaga
    19201 gtcatggaat ctttgccacc ggatacacct attaatattt tatcacattt tttaaatatt
    19261 tcgtaatctt ttatgaactg aaatatcttt ctctctattt cactgagttt tatcgtcatt
    19321 tgcgccgcca gtataaataa aaatcaatca tattatacat tactctgtca tataaaacaa
    19381 aactttgttt atcattaata gagatagact acaagctttt tactaaataa ttatcttaaa
    19441 aatctct
//
LOCUS       DAOSHR010000013        19246 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_2308_length_19246_cov_4.910218, whole genome shotgun sequence.
ACCESSION   DAOSHR010000013 DAOSHR010000000
VERSION     DAOSHR010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 19246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19246
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2308_length_19246_cov_4.910218"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..667)
                     /locus_tag="PLU28_01365"
     CDS             complement(<1..667)
                     /locus_tag="PLU28_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG33745.1"
                     /translation="MERYNIIDILGSYSYCQDFLVSFKDRFYRLRKLNSPEIFDMAAF
                     SKFETELEPFKIFGLVLPEFLKFDSERPELFYPYHNEKSFTGDISKKNYIRSLYFFFD
                     LIDKVVHGVNFIPKCLDIRDLCCDNNQNFYFIAPFFKNISDHSVYCLSENSEEALISV
                     MNRIIKDTDIRLRGTSRRLDLFFNDIQSRKIDCVSEYYSFYNSFFKDIWQIKPREPYF
                     VDRK"
     gene            complement(704..1717)
                     /gene="queA"
                     /locus_tag="PLU28_01370"
     CDS             complement(704..1717)
                     /gene="queA"
                     /locus_tag="PLU28_01370"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOG33746.1"
                     /translation="MYNLEEYDYKLDPMLIAQSPVEPRDSCRLLVVDRKEKTFEDKIF
                     RDISQYIGKDDIIVLNNTRVIPARLFGKKDSGAEIEVLLLEKLDKPNMWKCLVKPGGK
                     IKSSCRLIFSKNLSANVIEHLHDGSRIIEFEGIDVWKEICLIGQTPLPPYIHEKIDDP
                     EKYQTKYAKVEGAVAAPTAGLHFTSELIEKIKANGTKFAELTLHVGLGTFRPIKENDI
                     RNHEIHEEYYSIDKSTKEKIDETKKNGGRVIAVGTTVVRTLETVGIFPDRLFGKTDIY
                     IYPPFDFKYVDCLITNFHLPKSSLLLLVSAFSGYETVMDAYKKAVADRYRFFSFGDAM
                     FIK"
     gene            complement(1710..2705)
                     /locus_tag="PLU28_01375"
     CDS             complement(1710..2705)
                     /locus_tag="PLU28_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG33747.1"
                     /translation="MSIQNIKLVLEHRDYSNHCQRIRSDITRLNSWLVNPRTLNERKM
                     YLFYRTAGRNIEKKFISRHINDFQFLLMHIINNPNSSRFNDLKNYYKLTDDELQTLKF
                     LLYPKKFAPGGFNIDLKRYNIEFYCADAILKKLGFRDYIDLYALMKFVPYDADSPIIK
                     NFLNEVLCIDPLKTKKNYFMKVKEFYLSFSELERQIIDFRLSSISYYHYRLLSCPTVR
                     GAVIDGSNVIRHNSENKLQRLLDLIDNLFIDEMVFLPAVIVFDKNIEYVLDVSERESF
                     RNFVNDRFVYFNSPADDLILSISGKLDYYIVSNDKFKDYKANQEKILEIRRFLDV"
     gene            complement(2705..3784)
                     /locus_tag="PLU28_01380"
     CDS             complement(2705..3784)
                     /locus_tag="PLU28_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HOG33748.1"
                     /translation="MKIGILGLKSTGKSTVFSLLTDSVFDGSYKTDAVEKTAKVKDER
                     IDFFSDMFLPKKTTYATLNFIDIPGFDSKSDQKEKTRILQMIQAVDSIILVVRAFEST
                     VIPWPQDNDTTLKQLETLRTELIIRDMEVVENRLERLQADKKKKKSTPEMEKEEELLE
                     KIKQELDNGDFSSKLAINDEEKKLISSLALLTLKPIIVLLNIDEEQLKNASYKGREDI
                     LTDCGKQNFAYLELCGRIESDLVELPPEEKEVFMTELGIKKPGIDTLSKIVYDHIGLI
                     SFFTVGQDEVRAWTIEKNTSMKKAAGKIHSDLEKGFVKAEVMKSKDLIEYKSEEVLKS
                     KGLWKLAGKEEIVQDGDILNIRANT"
     gene            complement(3759..4253)
                     /gene="tsaE"
                     /locus_tag="PLU28_01385"
     CDS             complement(3759..4253)
                     /gene="tsaE"
                     /locus_tag="PLU28_01385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOG33749.1"
                     /translation="MITLYNMNEEKMMTLAAITAAQSFAGMKILLYGDLGSGKTFFVR
                     HFIKALSKDSNINVSSPTFPVQRIYDTAPRTSHVDLYRIADPDEIEYLDIFSDETMIY
                     LIEWAEYLDYLMPQEYLKIRIDYTDKLELRNITILSDSEKYSLIIKRIDEWRKNNENR
                     NSGT"
     gene            4407..5858
                     /locus_tag="PLU28_01390"
     CDS             4407..5858
                     /locus_tag="PLU28_01390"
                     /inference="COORDINATES: protein motif:HMM:NF021355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HOG33750.1"
                     /translation="MKKGLAFIIFLFFLVSAVAAQEKYFYVASGYEGFVIFKMDEDKS
                     IVPINVFKSGENILDLFVTKNTVFTAEGVAGMRILDITDKTNIKELAVFPCEDYCEGV
                     YVYSNYVYMSDGYKGLKIVDISNPSKPRLVSKLFVNDYIKNTRVSDGIAYMSTAGGRW
                     FMADISNPKKPLIYNFTNFGDYACDIKLYKNYAFVSDSSKGLKVLDISSAEDSYVKYN
                     FMDLDYVNSTWVFGDTLYVANGSSGVRAYDISDIQDITYLTDIDTENYAVSVYAYGEY
                     LYINDQYSIAIYDINSPAYPSFVSMVGFDSIENMYGYGESIYVYSNSSGQLSLLDVSD
                     PYNPVLKSTLMLESYYGQMQYINGNIIFSSSDNGLDIIDVSNPENIYLKYSVQLENNY
                     GYTSLYLNDNKLYAASDYFIDIFSLEDMSNIYKITSIYMNTALISMQISENKLYAACG
                     EDGALVFDISDISNPVLISDQVWIKFLSIVEQK"
     gene            complement(5864..6637)
                     /locus_tag="PLU28_01395"
     CDS             complement(5864..6637)
                     /locus_tag="PLU28_01395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOG33751.1"
                     /translation="MNIIDTHCHICSENLIPLRKEILDQCSELSMKIIEVGYTLESSE
                     SAIKLAAANNNIYASIGIHPSEYTAEKPQELYSRLNSLVLSPKVLAVGEIGLDFHWES
                     PKPFQYQLLELQLDLAKKRNLPVIFHVRQAYYEFYEFIKSYDFLSENAVVHCFSSDIS
                     WARPLLNLGFYLGFDGPITYPKNDALREVLAFCPQEKILCETDCPYMPPVPYRGKTNR
                     PAYIVEVIKKISSIKNINFSDMSDILYNNAHRLFRIDLS"
     gene            6798..7646
                     /locus_tag="PLU28_01400"
     CDS             6798..7646
                     /locus_tag="PLU28_01400"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOG33752.1"
                     /translation="MDLFVFDIDGTLLDSNVRMSDRTVRSLERISKQAEIVICTGRMC
                     SSTVNILKKYIPSFLGKANLITYNGGRVEDAKGTLIYESLIDKDIAVEISKYFFSKSI
                     YFQIYIDDQLYTYEDCDKIRDYSLHASVEYIIHEDLIELIRSAPSGPNKMLVIDEPER
                     LGSIQEFIGKYFSGVQSVMSFRNYLDIVPVDVSKGNALETMTLKLGADISEAFVFGDS
                     QNDISMLQLTENSFAMGNSTENVKNVCKYIIPTNDEEGVAQILEKILDSSDIDTFCNS
                     RYSSRN"
     gene            7579..8376
                     /locus_tag="PLU28_01405"
     CDS             7579..8376
                     /locus_tag="PLU28_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG33753.1"
                     /translation="MKKFLILLILILSATAATAAGTDFFCCSARVGCIYDSINEQVPT
                     QELSGYILDLSEFDQDLCYLSIGAMRNFEVTQKLARDIGVILKRKGFDFVIFGNLRLL
                     TKDSENKTDFFNSSYSLIAEHTYRMIRGFEAAGIFPVISVSRGDDKNAYKSLIQKSGS
                     MITFSYDDDSADIFFKENVFLSDKQNILSLAWEYPTETLSQSIMEVFKASIVLSGYRK
                     DLPILIYRQPSYNDDKSVIYFSQKVKDLAEQAVQGKISPSGNKFWNW"
     gene            8403..9020
                     /locus_tag="PLU28_01410"
     CDS             8403..9020
                     /locus_tag="PLU28_01410"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOG33754.1"
                     /translation="MNSSGEVEKLEKRVLELEGEVELYKQKLMEMDYLVEQYNELVKN
                     EFNSFDDFVQNLVTRKIIDENTRVYSKDFFDKLFAIYYQKSFELKKECGIIILTIPTL
                     RSSFGEAKKRMEREVGKVLRESVRIPLDFIARYREDRFVILLTDIVDEVFEKITVRIQ
                     DRLEMLCDRCGNIGVLKHYIPLERISLQDIFEELNEDKGADSYER"
     gene            9010..10293
                     /gene="murA"
                     /locus_tag="PLU28_01415"
     CDS             9010..10293
                     /gene="murA"
                     /locus_tag="PLU28_01415"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOG33755.1"
                     /translation="MKDNVPGTVMHVKGPQKATGTIKVSGAKNAVLPIMAATVLTEDT
                     VVLKNVPQIEDVNTMIEILTFIGKKVERTGDMLVILQDGHINPEVPYEPVRKMRASFN
                     IYGPLVVVAKKAKVSLPGGCALGARPVNFHIEGLLKLGIESTVEHGYVIGALKASEKN
                     VSINLPFPSVGATEHIITSAVLLDGVTTTISNCALEPEILDLVNFLKGMGADIRGGGT
                     PVVTVKGVKKLSGLEYSVIYDRIEAGTFAIMGALLGENLCIEGIEYDHLQSLFSLFDD
                     MNFNYRYDKHKKQMIVSAMDFSNLKSVSVETQPYPGFATDLQPQLMVMLSLIPGRSIL
                     TENVFKSRFNHVDELNRMGAKIKVEGNTAIIDGVTNLSGAQIMATDLRAAAALVIAAL
                     NACGESQISEIDHLFRGYEDIKNKFNNIGIKVDLK"
     gene            complement(10327..11274)
                     /locus_tag="PLU28_01420"
     CDS             complement(10327..11274)
                     /locus_tag="PLU28_01420"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOG33756.1"
                     /translation="MRSVFSDFTEDFFQIISFRKETWEDFWYEYKKQKPKVISEYLRL
                     NGISDADAVSRLLKLGRRYIDMAFWFRQERFSEVKYRIVKMLTDNPQRYQLDRGDYSV
                     HVICMFGEKPYEMIDTYAGTIIAVDFLYFYNHQKELTFETIVDQAILDFLSKVPFDKK
                     KADFCILLEEIKKIVNDTPDRAQAMSKIVSAMSKRINYYNWVGFYLTDTDQKDTLVLG
                     PYVGEPTEHVKIPFGSGICGQAAQNKTTFVVGDVSKETNYLSCSSKTLSEIVVPIKKS
                     DSSVVGEIDIDSHFIEPFDNKDTLFLEEVAYLISKKYYK"
     gene            complement(11294..11380)
                     /locus_tag="PLU28_01425"
     tRNA            complement(11294..11380)
                     /locus_tag="PLU28_01425"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11344..11346),aa:Leu,seq:cag)
     gene            complement(11430..12644)
                     /locus_tag="PLU28_01430"
     CDS             complement(11430..12644)
                     /locus_tag="PLU28_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG33757.1"
                     /translation="MMRKTFFILILIFLSVTAISQSISKDSMILVVMKDTLMTSKVAQ
                     KIRELGSYSYLVRPFKKVQEGFSTEWTEKVFDSLSFIGDVYLYIDYNDLSKKMSAWGT
                     FKDSSFYLEKDTLETDDWAYRFSVEILEYIASQRLVKDSTWKLLQLTFYKGVDEYPYF
                     QDNLLYYISDRYIGNRELYIWNMNTNLKEKIPLEQSSEYFPDITPDEKFIAFQSSMFG
                     KWDVVLYETASKSIKRISTSSYKNAYSPYCYSDTLILFSQDETDRSSEIWMYDTVEAT
                     SHRLTYVDNMLKFLPAKYDSNKIIFSGTNLITGDTGIYVINDDKTVSPLIDSQVNETD
                     CWSDSSKYIVFSKLIEGRYKIFLYNDGQSSIKTPSIDDDCYYPTFDKDRNFIFFTVYY
                     KDKEPDVFVVRF"
     gene            complement(12646..13086)
                     /locus_tag="PLU28_01435"
     CDS             complement(12646..13086)
                     /locus_tag="PLU28_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HOG33758.1"
                     /translation="MLKDLINEDLKCYMKQKNVAALNAVKLLKTDIKKTEVDTMTVLD
                     DEGVVKILKKQMKMRKESIEQYTAAGRTDLVQNEEEELKYLVKYLPQEMSDDQLKVII
                     DQVISELNATSSKDFGKVMKEVLARTKGKADNSKISGILKGLLH"
     gene            complement(13171..15165)
                     /gene="ligA"
                     /locus_tag="PLU28_01440"
     CDS             complement(13171..15165)
                     /gene="ligA"
                     /locus_tag="PLU28_01440"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOG33759.1"
                     /translation="MIPKNIVNEYEQLKNEVLAHNYKYFVLANPVISDEEYDKLFKRL
                     VEIESLYPQIKSPDSPSFRVGSAAVSGFAKIPHSIPMLSLDNTYNDEDIKAFNDRVLK
                     GLDGQSPEYLCELKIDGVSISLSYEKGILVQALTRGDGIAGEDVTINIKTLHSIPLKL
                     NADIDLQVRGEVYMPNREFERINNERDALGLAIFANPRNATAGTLKLLDSKEVAKRRL
                     SSFMYYIIDPSALKIETQLQALEYLDKIGFSVNKEYMLAKSIDSVTEYWRKWDTQRKT
                     LPYETDGIVVKVNSFTKQQILGQTIRSPRWAIAYKYTSEKKITRIIKINLQVGSTGII
                     TPVAELEPVQLEGTTVKRASLHNFEYIAERDIMENDYVLVEKAGGIIPQVIRSIPEKR
                     SGQEKKVIFPQSCPVCGYTTGKLSPQEVAVRCLNPLCPEKIIRSIENFVSKDAMNIDG
                     MGPKIIERLAQAGYIKDISDIYYLDELKIASLGSGIGIKTISNLMDQIEKSKNSSLER
                     LLCGIGIPLVGKKTARDLAINFKDLRVIAKSSIHELTTLEGIGEDMARSIVSFFSSQA
                     SFVLLEKLEKAGVNFIYKQDKVSDILKDIIVSVTGELLRFKRKEFEKYVHERGGIFSD
                     NLTKKTKILVIGQNPSSKAEKAKSWMIPSMSEEEFFEKYR"
     gene            complement(15162..15533)
                     /gene="acpS"
                     /locus_tag="PLU28_01445"
     CDS             complement(15162..15533)
                     /gene="acpS"
                     /locus_tag="PLU28_01445"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HOG33760.1"
                     /translation="MITGIGTDIVRIQRLTDPLIKKILSESEFRMYLSFSSEKRKKEF
                     AAGRFAAKEALIKASGSFIPFDQIEVLALPNGKPFFSKSTHDYLFSVLGNIKLHISIS
                     HEDEYATAFVLIERSVPEENL"
     gene            complement(15530..17596)
                     /locus_tag="PLU28_01450"
     CDS             complement(15530..17596)
                     /locus_tag="PLU28_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division FtsA domain-containing protein"
                     /protein_id="HOG33761.1"
                     /translation="MIFALDVGTRTLKAVLADIDEEENIIIHHCIMREHENRAMIDGQ
                     IHDIHKVAKGVNKVVTQLKELSGEKIDTVAVALAGRFLITSTGESSMDISSIKYIQED
                     TIRKIELDAVKTATENLDNFSSGMYCVGYSVLYYKLNDEWIKKLDGQRGEKASVKVIA
                     AFLPKNVVQAMMTVLEINNLTPVHITLEPIAAMNLIVPPDLRNLNIVMVDVGAGTSDI
                     AISSDGTIIGYGMVPLAGDEITEAITKALLVDFVTAENIKRNLSVQQEFVYKDILDFD
                     QKTSAQDIKNIIAPVLDEITSKISEKILELNGKPPVAVMIVGGGGKAAGFKEKLAEKL
                     SLIPNRVALKEASSLSTLVFENEKLNGSEFITPIGIANVALRKEGNVFNTVTVNGKTV
                     NMMMIGNDLTVMQVLLQAGYTINQLIGKPSPAISVEVDKKLVIKKGNMGKEAPIRING
                     IPATLKSFIKPSDSIEIGEPQSGPELILKVRDIINPGEYYINGEKKNFIPQVFRNGQK
                     LTADTIINDTDIISTKNPSLKELIGEFQKNVVFTLNAVPYEVPAGLAVSKNGEILSDS
                     YLINYNDRLEIHPVNLPSIREFTCTELKKRKVLFNDQILEMPCEEIIVKNSGKPIDLQ
                     TTVSEGSNYLVEIVPRDPTLLDILAFLSIETQKIHSFEFFINGNRAESFIQPLSENSV
                     VRFNYS"
     gene            complement(17601..>19246)
                     /locus_tag="PLU28_01455"
     CDS             complement(17601..>19246)
                     /locus_tag="PLU28_01455"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HOG33762.1"
                     /translation="LNERIIILCDEEKAEVYRILSELNFTTNKILNSLEKNIEIVSYL
                     DCLIGKARYCIAYKADFFVPASQASAISFVDLKHPLLEQKNAVPLSFILENRGIIITG
                     PNTGGKTVAIKSIGIAFVLTHSALPVTTVGCSIPFVENILTDIGDEQSIAQNLSTFSG
                     HLKNQKDIVSQADGKSIVIIDELGTGTDPIEGAALGNALIKDLLDKGAMIFITSHLSE
                     IKTFSLKEPRLSTASMSFDIDTLKPTYHFMIGVPGASHAIEIAQKMGFAESIITQAKE
                     NIDKEYIKHEELFSKLSTVYENVAAEKDELKRERIEVDRLKSQYLVQLEYLKKKELEK
                     ADKEIYNLRLKIKSIKSEIESLISMARQDISRNNFSSIKEYQKELSRIGTEIENLEPQ
                     EEPSEMIEDLKVGMTVVLPGKEEGIIEQIKSNKAVIKLLNSPVKLTYDLTQIRPSKKG
                     KTESIKDQSFQANPSAKQFFKELDIRGHTVDEAIPEIEQYISELIMYEKQFGYIIHGK
                     GTGKLSQGVWKYLRACRKIKGFRLGKTGEGGSGVTVVEV"
BASE COUNT         5770 a         3437 c         3554 g         6485 t
ORIGIN      
        1 ttttcctgtc gacaaaatac ggttctctag gttttatctg ccatatgtct ttaaagaagg
       61 agttataaaa actgtaatac tctgaaacac agtcaatttt tcttgattgg atatcgttga
      121 aaaatagatc taatctcctg gaggttcccc taagacgtat atcagtgtct ttaattattc
      181 tattcatgac agatataaga gcctcttcgg agttttcgga aagacaatag accgaatggt
      241 cagatatgtt tttaaagaaa ggagctataa agtaaaagtt ctggttatta tcacaacaaa
      301 gatccctaat atcaagacat ttaggtataa aatttacgcc atgaactact ttatcaatca
      361 gatcgaaaaa aaagtaaagc gagcgtatgt aatttttctt tgagatatcg cctgtaaaag
      421 atttttcatt atggtaaggg tagaatagtt caggtctttc agaatcaaac ttaagaaatt
      481 ccggaagcac aagtccaaag attttgaaag gttccagctc agtttcaaat tttgaaaaag
      541 ctgccatgtc aaaaatttca ggactattga gttttctcaa acggtaaaac cgatctttaa
      601 aagataccag aaagtcctga cagtatgagt aactccccaa aatatcaata atattatacc
      661 tttccatcta tgccccccaa aggttataag ctcttcaaaa agcttatttt ataaacattg
      721 catccccaaa tgaaaaaaat ctataccgat cagctaccgc ttttttgtaa gcatccataa
      781 cagtttcata tcctgaaaaa gcgcttacta atagtagtaa agaagattta ggaagatgaa
      841 agttagtaat cagacagtct acatatttaa aatcaaatgg aggatatata taaatatcag
      901 tttttccaaa cagtctgtcc ggaaatatcc ctaccgtctc aagggttctt actacagtag
      961 ttcccacagc aattacccgt ccgccattct tcttagtctc atctattttt tcttttgtgc
     1021 ttttgtctat actgtaatat tcttcatgta tctcatgatt acgaatgtcg ttttccttaa
     1081 ttggtctgaa agtacctaaa cctacatgaa gtgtaagttc ggcaaatttg gttccgttgg
     1141 ctttaatttt ttctattaat tctgaagtaa aatgtaatcc agcagttggt gctgccactg
     1201 ctccttctac ttttgcatac ttggtctgat acttttcagg atcatctatc ttttcatgta
     1261 tataaggagg aagtggggtt tgaccgataa ggcatatctc tttccacaca tcgatgcctt
     1321 caaattcgat aatcctgctt ccgtcatgga gatgctcaat tacatttgcc gaaaggtttt
     1381 tagaaaaaat aagtctgcaa gatgatttta ttttgcctcc tggttttaca agacacttcc
     1441 acatattcgg tttgtctagt ttttcaagaa gcagaacttc aatttctgct ccgctgtctt
     1501 tttttccaaa tagtcttgcc ggtattactc tggtattatt aagaacgata atatcatctt
     1561 ttcctatgta ttgtgaaata tctctgaaaa ttttatcttc aaacgttttt tcttttctgt
     1621 caacaactaa aagtctgcac gaatcccttg gttcaaccgg actctgcgca ataagcattg
     1681 gatcaagctt gtagtcatat tcctccagat tatacatcca aaaacctccg tatttcaaga
     1741 atcttttcct gatttgcttt ataatcttta aacttatcgt tagatactat ataatagtca
     1801 agctttcccg atatacttaa aataaggtcg tccgcaggtg aattaaaata gacaaacctg
     1861 tcgtttacaa aatttcggaa actttccctt tcggatacat ccaaaacata ttcaatgttt
     1921 ttatcaaaaa caatgactgc agggagaaaa accatttcgt ctataaagag gttgtctata
     1981 agatcaagca gcctctgaag tttattctcg gagttatgcc gtatgacatt acttccgtct
     2041 attaccgccc cacggactgt aggacagctt aaaagtctgt aatgataata agagatactg
     2101 ctgagtcgaa agtctataat ctgacgttcc agttcactga aggaaagata gaattctttt
     2161 accttcataa agtagttctt tttggttttt aacggatcaa tacagagaac ctcatttaaa
     2221 aaattcttta ttataggact gtccgcatca tacggaacaa atttcatcag cgcataaaga
     2281 tcaatataat ccctgaatcc aagttttttc aaaatggcat cagcacaata gaattcaata
     2341 ttgtatcttt ttaagtcgat attgaatcca cccggtgcaa actttttcgg ataaagaaga
     2401 aattttaagg tctgcagttc atcatccgta agtttatagt aatttttgag gtcattgaac
     2461 cgggaagagt ttggattgtt tattatgtgc ataagaagaa attgaaaatc attaatatgc
     2521 ctgctaatga attttttttc aatatttctc ccggcagtac gataaaaaag atacatcttc
     2581 ctttcgttaa gcgtccttgg attcacaagc caactgttga gccttgtgat gtcggatctg
     2641 attcgttggc agtgattgga ataatcacga tgctctagaa ccagctttat attctggatt
     2701 gacatcaggt gtttgccctt atgttaagga tgtctccgtc ctgtacgatc tcctcttttc
     2761 ctgcaagttt ccacaacccc ttggacttca gtacttcttc acttttatac tctataaggt
     2821 cttttgactt cataacttca gcttttacaa aacctttttc aagatcagaa tgtatctttc
     2881 ctgcggcctt tttcatggaa gtatttttct ctattgtcca tgctcttact tcatcttgcc
     2941 ctacggtaaa aaaggatatc agtccaatat ggtcgtatac aatttttgaa agcgtatcaa
     3001 tgccaggttt cttaatgcca agttctgtca tgaaaacttc tttttcttct ggaggaagct
     3061 ctacgaggtc ggattcaatc cgtccgcata gttccaggta agcaaagttc tgttttccac
     3121 agtcggtgag aatatcttcc cggcctttat aactagcatt tttaagctgt tcttcgtcaa
     3181 tattcaaaag gacaattata ggcttaagtg tcaaaagtgc gagggaactt ataagttttt
     3241 tttcttcgtc gtttatcgct agcttgcttg aaaaatctcc gttatcaagt tcctgtttga
     3301 ttttttctag aagttcctct tctttttcca tttcaggtgt gctcttcttt ttctttttat
     3361 ctgcctgaag tctctcaagt ctgttttcca caacctccat gtctcttatt atgagttctg
     3421 tgcgtaaggt ttcaagttgt ttcaatgtag tatcgttgtc ctgtggccat ggaataaccg
     3481 tgctttcaaa agctcgtact accagaataa ttgaatcgac agcctgtatc atctgaagaa
     3541 tacgagtttt ttctttttga tcggattttg aatcaaatcc gggaatgtca atgaaattaa
     3601 gtgttgcata cgttgttttc ttgggaagga acatatcgga aaaaaaatca attctttcat
     3661 cttttacttt ggcagttttt tcaactgcat ctgttttgta agagccatca aaaacagagt
     3721 ctgtaagtaa agaaaaaact gtggatttcc cggttgattt aagtcccaga attcctattt
     3781 tcattatttt tcctccattc atctatgcgt tttattataa gagagtattt ttcagaatct
     3841 gaaagtatag tgatatttct aagttcaagt ttatctgtat aatcaatacg gattttcagg
     3901 tattcttgcg gcataagata atcaaggtac tctgcccatt cgataagata gatcattgtc
     3961 tcatcagaga aaatgtcaag atactctatt tcgtcagggt ctgcgatcct gtaaagatca
     4021 acatgagagg ttcttggagc tgtgtcatat atccgctgca ccggaaaagt aggactagaa
     4081 acatttatat ttgagtcttt tgataatgct ttgataaaat gacgaacaaa aaaagttttc
     4141 ccgcttccca gatcgccgta aagcagtatc ttcatccctg cgaaagattg ggcggctgtg
     4201 attgcggcaa gagtcatcat tttttcttca ttcatattgt aaagagtaat catgtaatcc
     4261 tccgatttct ttttcacaaa taatatttta tcattaaatt ctctgtttta taaggcaatt
     4321 aattttactt agtttgagaa acaaaaaatt tacatagtca agatataata tttacaatag
     4381 actcaaaaac taaaggagga atcattatga agaagggtct ggcctttatt atatttttat
     4441 tttttttggt ttcggctgta gcggcacagg aaaaatactt ttatgttgca agcggatatg
     4501 aaggatttgt tatattcaaa atggatgaag ataaaagtat agttccgatt aatgtcttta
     4561 aaagcggtga aaatatttta gatctgtttg ttacgaaaaa cacagtattt acggctgaag
     4621 gtgttgccgg aatgagaatc ctggatatca ctgacaaaac aaatattaag gagcttgccg
     4681 tttttccctg tgaagattac tgtgaggggg tttatgtata ctccaattat gtttatatgt
     4741 cagacggtta taagggtctt aagatagtag atatatctaa tccgtctaaa ccaaggctgg
     4801 taagcaagct ttttgtcaac gattatataa agaatacaag agtttctgat ggtatagcct
     4861 atatgagtac tgcaggcgga agatggttta tggccgatat aagcaatccc aaaaaacctt
     4921 tgatatataa ttttacaaac tttggggact atgcttgtga tataaagctg tacaaaaact
     4981 atgcctttgt ttcggattca tcaaaaggct tgaaggttct tgatatttca tcagcagaag
     5041 attcttatgt aaaatacaat tttatggatt tggattatgt taacagcaca tgggtatttg
     5101 gagatacgct ttatgttgct aatggaagca gtggagtaag agcgtacgat atttcagata
     5161 ttcaggacat aacctatctt actgatattg atactgagaa ttatgccgta agtgtatatg
     5221 cttatggtga gtatctttac ataaatgatc agtattcgat agccatatat gatataaatt
     5281 cacctgcgta tccgtcattc gtttcaatgg ttggttttga tagtattgaa aacatgtatg
     5341 gatacggtga aagtatctat gtatacagta acagttcagg acaactaagt cttttggacg
     5401 tttcagatcc atacaatcct gtattgaaaa gcacgctgat gcttgagtca tattatggac
     5461 agatgcagta cattaacggc aatattattt tttcatcatc agataatggc ttggatataa
     5521 tagatgtaag caatcctgaa aatatttatc taaagtattc cgtacaactt gaaaataatt
     5581 atgggtatac atcattgtat ctcaatgata acaaactata tgctgcgagt gattatttta
     5641 ttgatatatt ttcgcttgag gatatgagta atatatataa gataacaagt atctacatga
     5701 atacagctct gataagtatg cagatatcgg aaaacaaact gtatgcagcc tgtggagaag
     5761 atggggcatt ggttttcgac ataagcgata tatcaaatcc ggtactgata tcagatcagg
     5821 tatggataaa gtttctttct attgtcgagc aaaagtaaga tttttacgag agatcaatcc
     5881 tgaaaagtct gtgagcatta ttatagagta tatcagacat gtctgaaaaa tttatattct
     5941 tgattgaaga gatcttttta attacttcaa ctatataggc tggtctgttt gtttttcctc
     6001 tgtatggaac aggaggcata tacggacagt ctgtttcaca caggattttt tcttgtgggc
     6061 agaacgccag aacttcccgc agagcatcat ttttaggata agtgatagga ccatcaaagc
     6121 caagataaaa gccaagattc aaaagtggac gagcccatga gatatctgat gaaaaacagt
     6181 gtactacagc attttctgat aagaaatcat aagattttat aaattcataa aactcataat
     6241 aagcctgcct cacatggaaa ataacgggca ggtttctttt ttttgccaga tcaagctgaa
     6301 gttcaaggag ctggtactga aaaggtttag ggctttccca atgaaaatca agaccaatct
     6361 ctccaacagc aagcactttg ggagataaga ccagactatt taggcgggaa tacagctctt
     6421 gaggtttttc agcagtatac tcgctgggat gtataccaat agatgcgtat atattattgt
     6481 ttgccgcagc gagttttatg gctgattctg aactttcaag tgtatagccg acttctataa
     6541 ttttcatact aagctctgaa cactgatcaa gtatctcttt acgcaagggg ataagatttt
     6601 ccgagcagat atggcaatga gtatctatta tattcatagg gcaacatctc cttataaaaa
     6661 aagtttatca taaagatgtt ttttctgtat taaaaaaact tcaagtgttt tgtttttctg
     6721 cttatgcaaa agatgcccag agatagtata attgatttta tattaagatt tatgatagaa
     6781 tctagcagga ggaagttatg gatttatttg tttttgatat tgatggaaca ttacttgact
     6841 caaacgtaag aatgagcgac agaacagtca gatcgctgga aagaataagt aagcaagctg
     6901 agattgtaat ctgtacggga agaatgtgtt cttcaacggt taatatactg aaaaaatata
     6961 ttccctcatt tttgggtaaa gcaaatctga taacgtataa tggcggcaga gtggaagatg
     7021 caaaaggtac gctcatatat gaatcattga tcgataaaga tatagctgtg gaaatttcaa
     7081 agtatttttt tagtaagagt atttactttc agatatatat agacgatcag ctttatacat
     7141 atgaagattg tgacaagata agagattatt cattacatgc atcagttgaa tatataatac
     7201 acgaggactt aattgaactt ataagatctg caccatcggg accaaataaa atgcttgtta
     7261 tagatgagcc tgaaagactc ggtagtatcc aggagttcat tgggaagtac ttctcgggcg
     7321 ttcagagtgt aatgtctttc agaaattatc ttgatattgt tccggttgac gtgtctaagg
     7381 gaaatgcatt ggaaactatg accttaaaac ttggagcaga tatatcggaa gcttttgttt
     7441 ttggagattc acagaacgat atttcaatgc tccagcttac agaaaattct tttgcgatgg
     7501 gaaattctac agaaaatgta aaaaacgtgt gcaagtatat tattcctaca aatgatgaag
     7561 aaggagttgc tcagatactt gaaaaaattc ttgattcttc tgatattgat actttctgca
     7621 acagccgcta cagcagccgg aactgatttt ttttgttgtt ccgcaagggt tggatgtatt
     7681 tatgacagta taaatgaaca ggtgcctact caagagttga gcgggtatat tcttgatcta
     7741 tcagaatttg accaggatct ttgctatctt agcattggag caatgcgcaa ctttgaggta
     7801 actcagaagc ttgcgagaga tataggagta attcttaaac gtaaaggatt tgattttgta
     7861 atttttggaa atttacggct acttacaaaa gattcagaga ataagacaga ttttttcaac
     7921 tcttcgtatt ctctgatagc agaacatacc tacagaatga tccggggatt tgaagcagcc
     7981 gggatttttc cggtaatttc agtatctcgc ggggatgata aaaatgccta caagtctctt
     8041 attcaaaaaa gtgggagtat gattactttt tcgtatgatg atgattcagc agatatattt
     8101 tttaaggaga atgttttttt aagtgacaaa cagaatatcc tttctctcgc ttgggagtat
     8161 ccgacagaaa ctctttccca gagcataatg gaagtgttta aggcttcaat agtattgagc
     8221 ggctacagaa aagatttacc cattcttatt tacagacagc cttcatataa tgatgataag
     8281 tccgttattt atttttcaca gaaagtaaaa gatcttgcag agcaggcagt tcagggaaaa
     8341 ataagtccat ctggaaataa attctggaat tggtaaaata tctacaggag gttaaacgac
     8401 ctatgaatag ttctggggaa gttgaaaaac ttgagaaaag agttttggaa cttgaggggg
     8461 aagtagaact ttataaacag aaacttatgg agatggacta tctggtggag cagtataatg
     8521 aactggtgaa aaacgagttt aattcttttg atgattttgt acagaatttg gttaccagaa
     8581 agataataga cgaaaacaca agagtttatt caaaagattt ttttgataaa ctgtttgcca
     8641 tatactatca gaagtctttt gagctaaaaa aagaatgtgg gataataatt cttactatcc
     8701 ctacgttgag aagcagcttc ggtgaagcta aaaagcgtat ggaaagagaa gtcggaaaag
     8761 ttttacgtga aagtgtaaga ataccgcttg atttcatagc cagatatcgc gaagatagat
     8821 ttgtaatact gcttacagat attgttgatg aggtgtttga aaagataacg gttagaattc
     8881 aggacaggct cgaaatgctt tgtgacagat gtgggaatat tggagttctt aaacactaca
     8941 taccgttaga aagaataagt cttcaggata tatttgaaga gctgaatgag gataaagggg
     9001 cagatagtta tgaaagataa tgttccaggt acggtaatgc atgtaaaagg tccgcagaaa
     9061 gctactggga cgataaaggt gtccggtgcg aaaaatgctg tactccctat aatggctgct
     9121 acggttctta cagaggatac ggtagttctg aaaaatgttc ctcagattga ggatgtaaat
     9181 actatgatag aaatattgac ctttataggc aagaaagtgg aaagaactgg tgacatgctg
     9241 gtcattctgc aggatgggca tattaaccct gaagtacctt acgagccggt aaggaagatg
     9301 agggcatctt ttaatatata cggtcctttg gtagtggtgg caaaaaaagc aaaggtttcg
     9361 cttcctggag gatgtgctct tggcgcaagg cctgtgaact tccatattga aggcctttta
     9421 aagcttggga ttgagagtac tgtagaacac ggctatgtta tcggtgcact aaaggcatca
     9481 gaaaaaaatg taagtataaa tcttccattt ccgagtgttg gagcaactga acatattatt
     9541 acttctgctg ttcttttgga tggagttact accacaataa gcaactgtgc gcttgagccc
     9601 gaaattttgg atttagtgaa ttttcttaag ggaatggggg cagatattcg cggaggtgga
     9661 actcctgtcg taacagtgaa aggagtaaaa aaactttccg gattagagta ctctgttatc
     9721 tacgacagaa tagaagccgg aacatttgct attatgggag cgctgctagg tgaaaattta
     9781 tgcatagaag gtatcgaata tgatcatctt cagagccttt tttctttgtt tgatgatatg
     9841 aactttaact atagatacga caaacataaa aaacaaatga tagtttctgc aatggatttt
     9901 tcaaatctga aaagtgtttc ggttgaaaca caaccatatc ctggttttgc gacagatctt
     9961 cagcctcagc ttatggtaat gctatcgctt attccgggac ggtcaatttt aactgaaaat
    10021 gtatttaaaa gtcgttttaa tcatgttgat gaattgaata gaatgggtgc aaaaattaaa
    10081 gtagaaggaa atacggccat aatagacgga gttacaaatc tttccggggc acagataatg
    10141 gcaacagatc ttagagcggc tgcagcactt gttattgctg cacttaatgc ttgtggagaa
    10201 tcccagatat cagaaataga ccatctattc agagggtacg aagatataaa gaataaattc
    10261 aacaatatcg gaataaaagt ggatttaaaa tgaaaacaac cgctgagaat cgctctcggc
    10321 ggttgtttat ttataatatt tttttgatat tagataagct acctcttcaa gaaaaagcgt
    10381 atccttattg tcaaaaggtt caataaaatg agagtctata tctatctcac ctactacaga
    10441 actgtccgat ttttttatag gaacaactat ttccgaaaga gtcttggagc tacaagaaag
    10501 atagttggtc tccttcgaaa catctcctac aacaaaagtt gtcttatttt gagctgcttg
    10561 tccgcatatt ccgcttccaa aaggtatttt tacgtgttct gtgggttctc cgacatacgg
    10621 tcccagaact agggtatctt tttgatcagt atctgtaaga taaaagccta cccaattgta
    10681 ataatttatt cttttggaca tggccgacac aattttagac atagcctgag ccctatctgg
    10741 tgtatcgttt actatctttt taatctcttc cagtaagata caaaagtcgg ctttcttttt
    10801 atcaaaaggt acttttgaaa gaaaatcaag tatagcctga tcaacaatag tttcaaaagt
    10861 cagttccttt tgatgattgt aaaaataaag gaaatcaacc gctattattg ttccggcata
    10921 agtatctatc atttcatagg gtttttctcc aaacatgcat attacatgaa cagaataatc
    10981 tccacgatca agctggtacc tttgcggatt gtctgtaagc atcttaacta tacgatactt
    11041 aacttctgaa aagcgttctt gtctgaacca gaaagccata tcaatatatc ttctcccaag
    11101 ctttagtaac ctgcttactg catcagcatc agatataccg ttgagacgaa ggtattcgct
    11161 tataacttta ggcttttgtt ttttatactc ataccagaaa tcttcccagg tttcttttct
    11221 gaaagagatt atctgaaaaa aatcttctgt aaaatcgctg aaaacactcc tcatgtaaca
    11281 cctcataaaa aaatggtgcc ggaagcggga cttgaacccg cacaagcctg agctcataag
    11341 atcctgaatc ttacgcgtct gccaattccg ccatcccggc acaactatta tattttacta
    11401 taaaaattga agaatgtaaa gagttttttt tagaatctta caacaaatac gtcaggttct
    11461 ttatctttat agtaaactgt aaagaagata aaatttctgt ctttgtcaaa agtcgggtaa
    11521 taacagtcgt catcaatgga gggcgttttt atagaactct gtccatcatt gtaaaggaaa
    11581 atcttataac gaccttctat aagctttgaa aagacaatat acttgcttga atctgaccag
    11641 cagtctgtct catttacctg tgaatcaatc agcggtgata ccgttttatc atcgtttata
    11701 acataaattc cggtatcgcc tgttattaga ttggttccgc tgaaaataat cttatttgag
    11761 tcgtatttag ccggaagaaa cttaagcatg ttatcaacat atgtcagccg gtgtgaagtt
    11821 gcttctaccg tatcgtacat ccaaatctcc gaagatctgt ctgtttcatc ctgagagaaa
    11881 agaataaggg tatcagaata acagtatggg gagtaggcat ttttatagct acttgttgaa
    11941 atccttttta tggatttgct tgctgtttca tagagtacta catcccattt tccaaacatt
    12001 gaactctgaa aagcaataaa tttttcgtcc ggtgtaatat ccggaaaata ttccgaggac
    12061 tgttctaaag gtattttttc tttcagattt gtattcatat tccagatata aagttccctg
    12121 tttcctatat atctgtcgga tatataataa aggagattat cctgaaaata ggggtattca
    12181 tcaacgcctt tatagaatgt caactgaagc agcttccatg tactgtcttt gacaagccgt
    12241 tgggaagcaa tgtactcaag aatctcaaca ctgaacctgt atgcccaatc atctgtctct
    12301 aaagtatctt tttcaagata aaacgaagaa tctttaaagg ttccccaagc actcattttt
    12361 ttggataaat cattgtaatc aatgtataaa tagacatctc cgatgaagct gagactatcg
    12421 aaaacttttt ctgtccattc ggtcgaaaag ccttcttgaa ctttcttaaa aggtctgacc
    12481 agataactgt aacttccaag ctcacgtatt ttttgtgcaa cctttgaagt cataagagtg
    12541 tctttcatta caacaagaat catggagtcc tttgaaatac tttgagatat agctgttacg
    12601 gataaaaata tcagtataag tataaaaaag gtttttctca tcatcttagt gtaaaagtcc
    12661 ctttagtatc ccgcttattt ttgaattgtc tgcttttcct ttggttcttg ccagaacttc
    12721 tttcatgacc tttccaaaat cctttgaaga ggtggcattc aattctgata taacctggtc
    12781 gatgatcact ttgagctgat catctgacat ttcctgtgga agatacttta ccaaatattt
    12841 aagttcttct tcttcattct gtactagatc agttctgccg gcggcggtat actgttctat
    12901 agattctttt ctcattttca tctgtttttt aagaatctta accacacctt cgtcatccag
    12961 cacggtcata gtatcaactt cggttttctt tatgtcggtt tttaaaagtt ttactgcatt
    13021 aagtgctgca acattttttt gtttcatata acatttcagg tcttcgttta ttagatcttt
    13081 cagcatactt ctcctccttt agcgtaaaaa caaatataag acagtatata tactctatta
    13141 tataccggga ttttacagtt ttcataaagt tcatctgtat ttttcaaaaa attcttcttc
    13201 cgacattgac gggatcatcc aacttttggc cttttctgct ttagaagatg gattttgccc
    13261 tattaccagt atcttggttt tctttgtaag attatctgaa aaaatgcctc cgcgttcatg
    13321 tacgtacttt tcaaactctt ttcttttaaa ccggagtagt tctccggtta ccgaaactat
    13381 gatatcttta agtatatcag aaaccttatc ctgcttatag ataaagttta ctccggcttt
    13441 ttccagcttt tccaaaagca cgaaactggc ttgggaagaa aaaaaagaga ctattgaacg
    13501 tgccatatct tctccgattc cttctaaggt tgtcagttca tgaatgcttg atttagcaat
    13561 tactctgaga tctttaaaat ttattgccag atctctggcg gtttttttcc ctaccaaagg
    13621 tattcctatt ccacacaaaa gtctttcaag actagaattt ttggattttt ctatctgatc
    13681 cataagatta gatatggttt tgattcctat tccacttcca agggaagcta tcttcagttc
    13741 atcaagatag tatatatcag agatatcctt tatgtaccct gcctgagcaa gcctttcaat
    13801 tattttagga cccataccgt ctatattcat ggcatctttg gatacaaaat tttcaatact
    13861 gcggataatt ttttccggac aaagcgggtt aaggcatcgc actgcaactt cctgtggaga
    13921 aagttttcct gtggtataac cacataccgg acaggattgc ggaaaaatga cttttttctc
    13981 ttgtccactt cttttttcag gtattgaacg gattacctgg ggaattattc ctcctgcttt
    14041 ttcaaccaga acgtaatcat tttccattat atcgcgttct gctatatact caaagttgtg
    14101 aagacttgct cgttttactg ttgttccttc aagttgcacc ggttcaagct ccgctaccgg
    14161 agttattatt cctgtgcttc ccacctgcaa attgattttg attatccgag taatcttttt
    14221 ttccgaagta tatttatatg ctattgccca tcttggggat cttatggttt gacctaaaat
    14281 ctgttgctta gtgaatgaat taacttttac aacaatgccg tcagtttcat atggcaaagt
    14341 tttccgttgt gtatcccatt ttctccagta ctctgttaca gaatcaatgc ttttggcgag
    14401 catatactct ttatttacgc taaaacctat tttatcaaga tactcaagag cctgcagctg
    14461 tgtctcaatt tttaaggcag aaggatcaat aatatagtac ataaatgaac taaggcgtct
    14521 cttggcaacc tctttgctgt caaggagttt tagagtgccg gctgttgcgt tccggggatt
    14581 agcaaaaatt gctaatccta aggcatcacg ttcgttattt atccgttcaa actctctgtt
    14641 aggcatatag acttcgccgc ggacttgtag atctatatca gcattcaact taagaggtat
    14701 cgaatgcaag gttttaatat ttattgtgac atcttcgcca gcaattccgt cacctcttgt
    14761 aagtgcctga acaagtattc ctttttcgta actaagagat attgaaactc catcgatttt
    14821 cagttcacat agatactccg gagattgccc atcaagacct ttcaataccc tgtcattaaa
    14881 agcttttata tcctcgtcat tatatgtgtt gtcgagcgaa agcatcggta ttgaatgagg
    14941 aatctttgca aaaccagata ctgcggccga gcctactctg aaagatggag agtcgggaga
    15001 ttttatctgg ggataaagag actcgatctc aaccagcctt ttgaaaagct tatcatactc
    15061 ttcatctgaa ataacaggat tggcaaggac gaagtattta taattgtgtg caagtacttc
    15121 gtttttcaat tgttcatatt cgtttactat atttttcggg atcatagatt ttcttctggc
    15181 acactccttt ctataagtac aaaggctgtc gcatattcat cttcatgcga aatggaaata
    15241 tgaagcttaa tgtttccaag cacagaaaaa agataatcat gagttgattt tgagaaaaaa
    15301 ggcttaccgt tcggaagtgc aagaacttcg atctgatcaa aagggataaa tgaaccgctg
    15361 gctttgatca aagcttcttt agccgcaaag cgtcctgccg caaactcttt tttccttttt
    15421 tccgaggaaa aagaaagata catcctaaac tcgctttcag aaaggatttt ttttattaat
    15481 ggatctgtaa gcctttgaat cctgactatg tccgtaccta tgcctgttat catgagtaat
    15541 taaaccttac aacagaattt tcagaaaggg gctgaataaa actttctgct ctgtttccgt
    15601 tgataaaaaa ttcaaaagaa tgaatttttt gagtttcaat tgatagaaat gccagtatat
    15661 caagaagagt cggatctcta ggaacaattt ctacgagata gttcgagcct tcgcttactg
    15721 ttgtttgcag atcaattggt ttgccggagt tttttacaat gatctcctca catggcatct
    15781 ccaaaatctg atcattgaag agtacttttc tttttttcaa ttctgtacag gtaaactccc
    15841 ttattgaagg aaggttgacc ggatgtattt ccagcctgtc attgtaattt ataagatatg
    15901 agtcagaaag tatttctcca tttttgctta cagccagacc tgcaggcact tcataaggaa
    15961 ctgcgttaag tgtgaatact acatttttct gaaactctcc tatcaactct tttaaagaag
    16021 gatttttggt tgaaatgatg tcagtatcgt ttataatagt atcggctgta agtttttgtc
    16081 cgttgcggaa aacctgagga atgaaatttt ttttctcacc gtttatatag tattcacccg
    16141 gattgattat gtctcgtacc ttgagtataa gctcggggcc agactgaggc tctcctattt
    16201 ctatcgaatc agaaggcttt ataaaagatt ttagagttgc cggaattcca ttgattctta
    16261 ttggggcttc ttttcccata tttccttttt ttatcaccag tttcttatca acttccacag
    16321 atatggcagg agaaggtttc cctatcagtt ggtttatagt gtatcctgct tgaaggagaa
    16381 cttgcataac ggttagatcg tttcctatca tcatcatatt tacagttttt ccatttacgg
    16441 ttacagtgtt aaaaacattt ccttcttttc taagggcaac gttggcgatt cctatgggag
    16501 tgataaactc gcttccgtta agcttttcat tttcaaaaac aagagtactt aaactcgaag
    16561 cttctttcaa agctactcta tttggtataa gtgaaagctt ttcggccagt ttttccttga
    16621 aaccagctgc ctttccgcca cccccgacaa tcataacggc tactggaggt tttccgttaa
    16681 gttccagaat tttttcagat atttttgaag taatttcatc cagtacggga gcaattatat
    16741 tttttatatc ctgagcagaa gttttctgat caaaatcaag aatatcctta tatacaaatt
    16801 cctgttgtac tgaaagattt cttttgatat tttctgcagt tacaaaatca accaaaagtg
    16861 ctttggtaat tgcctcagta atctcatcac cggcaagggg aaccataccg taacctatga
    16921 tggtaccatc tgaagaaatt gcaatatcac ttgttccggc tccgacatca accattacga
    16981 tattaaggtt tcgaagatca ggaggaacta ttaagttcat agcggctata ggttcaagtg
    17041 ttatatgtac cggagtcagg ttatttattt ctagaactgt catcatggcc tgaacaacgt
    17101 ttttgggtag aaatgcggca attactttta cactggcttt ttcgccgcgc tgcccgtcaa
    17161 gtttctttat ccactcatcg ttaagcttgt agtacagaac tgaataaccg acacaataca
    17221 taccggatga gaagttgtca agattttcag tggccgtttt gacagcatca agttctattt
    17281 tgcggatagt atcttcctgg atatatttta tagaagaaat atccatagaa gactctccgg
    17341 tagaggttat caaaaatctt ccggcaagag ctacggctac ggtgtcaatt ttttccccag
    17401 aaagttcttt aagctgggtg actactttat tgactccttt ggcaacctta tgaatatcat
    17461 gaatttgtcc gtcaatcata gcacggtttt catgttctct catgatacag tggtgaatta
    17521 taatgttctc ctcttcatca atatctgcca gaacagcctt taaagttctt gttcctacat
    17581 ccagagcaaa tatcattaag ctacacctcc actactgtta ctccacttcc accctcgcct
    17641 gtttttccaa gtctgaagcc ttttattttt ctacaggctc tgagatactt ccatactcct
    17701 tgtgaaagct ttcctgttcc ttttccgtga ataatgtatc caaactgttt ttcatacatt
    17761 atcagttctg atatatactg ttctatttca ggtatggctt catcaactgt atgcccgcga
    17821 atatcaagct ctttaaaaaa ctgcttagcc gaaggattgg cctgaaaaga ctgatctttt
    17881 atggattcgg tttttccttt tttggaaggc ctgatctgag taaggtcata tgtgagcttg
    17941 acaggcgagt taagaagctt aataaccgct ttgttgcttt tgatctgttc tattattccc
    18001 tcttcttttc ccggaagaac cacggtcatt cctactttta aatcttcaat catttctgaa
    18061 ggttcttcct gaggttcaag gttttctatc tcggttccta tacgagagag ctccttttga
    18121 tactctttta tggaggaaaa attatttctg cttatatcct gccttgccat acttataaga
    18181 gattcaattt cagacttaat actcttaatc ttcagtctaa gattatagat ttctttatca
    18241 gccttttcaa gttctttctt ctttaagtat tcaagttgta caagatactg ggatttaagt
    18301 cggtccactt caatacgttc tcttttgagt tcatcctttt ctgctgcaac attttcatat
    18361 acagtgctga gcttggaaaa aagttcttcg tgttttatgt actctttatc tatattttct
    18421 tttgcctgag ttatgatact ttcagcaaaa cccatttttt gggctatttc tattgcatga
    18481 gatgctcctg gaactcctat cataaaatga taagttggct ttagagtatc gatatcgaat
    18541 gacattgatg cggttgaaag cctgggttcc ttgagagaga atgtttttat ttcagaaaga
    18601 tgagaggtga tgaatatcat agctcctttg tccaataaat cttttataag agcattgcct
    18661 aaagctgctc cttcaatagg atcagtacca gttccaagtt catcaataat gactatgctc
    18721 tttccgtctg cctgggagac tatatctttt tgatttttta ggtgtcctga aaaggtgctc
    18781 aggttttgtg caatactttg ttcatcacct atatcggtaa gaatattttc aacaaaaggg
    18841 atactgcatc cgacagttgt tacaggcagg gcactgtgag tcagaacaaa agctattcca
    18901 attgatttta tagctacagt tttaccgcca gtattaggac ctgtaattat tatgccgcgg
    18961 ttttcaagga taaacgaaag tggaacagca tttttctgct caagcagagg atgcttaaga
    19021 tcaacaaagg aaattgccga ggcttgagaa gcaggtacaa aaaaatctgc tttgtaagct
    19081 atacagtaac gtgcttttcc tattaaacag tctaaataag atacgatctc aatatttttt
    19141 tcaagtgagt ttaaaatctt gtttgtagtg aagttcagtt ctgaaagaat gcggtatact
    19201 tcggcttttt cttcatcaca aagtatgatt atgcgttcgt tcagtt
//
LOCUS       DAOSHR010000014        19031 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_2339_length_19031_cov_5.656145, whole genome shotgun sequence.
ACCESSION   DAOSHR010000014 DAOSHR010000000
VERSION     DAOSHR010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 19031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19031
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2339_length_19031_cov_5.656145"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="PLU28_01460"
     CDS             complement(<1..286)
                     /locus_tag="PLU28_01460"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOG33763.1"
                     /translation="MLTKIDKHSGVPAYLQIMNQIKKEIFLGRFTDGSQLPPVREMEN
                     IFGVNMNTIMRALEYLQFEKIVEAKHGIGYFITSDVIIDPDIPKQIMEFVR"
     gene            complement(299..1231)
                     /locus_tag="PLU28_01465"
     CDS             complement(299..1231)
                     /locus_tag="PLU28_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG33764.1"
                     /translation="MLKEYEKLARFLIVIVISLAAVLVPFVPLKIILASVAIFLYAMN
                     IKRFSTLFIVLAVIFVIIPVLFLAISRDYNLVSRFFNSSSWRYIYFSDWESEYSHEGY
                     TVVEDGALLPALSYRVSGGALNIKFTSDENNPRAIGKVAFKKKGDVNNISALDYVRST
                     SEAATISLPSGERIEILDIQNSAVSISGEFYAQRVSADTQVCTIDATLTAEKTVILIA
                     DDLKAKGMVKAEKMIINGNICELDLNVSEVKTFNIDSLYANGRIKYTDSWEGVRILSI
                     SSNSGRIEVMQPRNNEGTLDIRTQDGVTVLSTLY"
     regulatory      1413..1582
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            1630..2247
                     /locus_tag="PLU28_01470"
     CDS             1630..2247
                     /locus_tag="PLU28_01470"
                     /inference="COORDINATES: protein motif:HMM:NF014612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-8X methylmutase"
                     /protein_id="HOG33765.1"
                     /translation="MLEYIKDPAMIERKSFEIIAEKLDGKLSHLGSIEQNIIKRVVHC
                     SGNLDLWDKLNFSEDFFESFLSFLRYKTVYTDVNMVKSGINKKIIPELQVYCFIDEPE
                     IAELSKEQKISRAYLSMQKALRANPGIFVIGNAPTALFKLLESTEKLFIIGVPVGFVG
                     AAQSKELLVGSVHSYVTLSGTFGGSNIAAAIMNALLKEAYNAAIR"
     gene            2231..3343
                     /gene="cbiD"
                     /locus_tag="PLU28_01475"
     CDS             2231..3343
                     /gene="cbiD"
                     /locus_tag="PLU28_01475"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase
                     CbiD"
                     /protein_id="HOG33766.1"
                     /translation="MQQYVNVNGQLLRRGFTTGTCACAALKACILWTLYDKKDPEVSV
                     RLNEGSLVNLKIESYEIMEGKKYAFIRKDAGDDSDITNHCLIYVSYKATEPGTGFVFT
                     SAEGVGLVTKKGLDVRQGEYAINPGPRKMMKSVLQEYGINDAIITVNVKDGQKIAIKT
                     LNSSLGIVGGVSILGTSGIVEPMSEEAWRESLVPQIRMIGSYFSSITLLSGGKSRKIY
                     ETSTFFKNDFLLCGNFFGFSIEKAIENHIKTIHIMGSFQKIIKLAGLNLNTDSRLSDS
                     KNELLALMYIMFKKTYDEEMVKKILSLNPVYSIIELFDEHPGESKAFFEFCGSYIAQK
                     LEKRFNQPFIITLCEKESILSHTGGLLYALHNGNTD"
     gene            3315..4520
                     /locus_tag="PLU28_01480"
     CDS             3315..4520
                     /locus_tag="PLU28_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG33767.1"
                     /translation="MLYIMGTQINCEENFTTRQLDIISKADFFAGFSFSLQIAKKVNP
                     TAEFYCMNIYDSLKEENIDHLVSILRKYKNPVIMVSGNPLMFSYTKNITSKLNQDEYE
                     VLVSPSSVDYLAQKTKTILNGLCYVSGHNSHNLQKTYNEVLFFLNMGRPVAYFVKNMD
                     DFSNLSTLLGNVKVTIAAGFELGTPQEKIFNFESQNFPDFIHGRWILILQPAQKDFLD
                     SFPKNSDLNCNNIPVSREWTRFLCVNSLNRHDKHDIIWDLGAGSGATSIYISSFYGRN
                     CSVKAIEKEKNRFGHLVENTAMNPEIFCINKDFKNILRNLDRPDIVHLGGGIDRESLS
                     LISQYMPKGSILISSATTQQSLCTVLQSPGYAFEYSMYAKFSSNNISNKDAFKGEYVF
                     YCIRGEKID"
     gene            4513..5223
                     /locus_tag="PLU28_01485"
     CDS             4513..5223
                     /locus_tag="PLU28_01485"
                     /inference="COORDINATES: protein motif:HMM:NF012799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-4/precorrin-4
                     C(11)-methyltransferase"
                     /protein_id="HOG33768.1"
                     /translation="MISFIGCGPGSKDFLTIKGYERIKTADVIIYAGSTVDHLLTDIS
                     GAGKIFDSSSMTLEDIEKVFLDNRSLNIARMHSGDTSLYSAIDEEIALAEKANIAFEV
                     IPGISAYSYFASKLGIELTSPGICQSVVLTRLNGRTPVPENIESFFGEQPSCAIYLSG
                     SKPYEVLSLLKKHYDKNAMLAIGTHLAREQERIEVKPLHSWHDIDFPSNLTLFLIFRK
                     GSIKSCLYDKNFSHGMRK"
     gene            5220..6215
                     /locus_tag="PLU28_01490"
     CDS             5220..6215
                     /locus_tag="PLU28_01490"
                     /inference="COORDINATES: protein motif:HMM:NF014006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HOG33769.1"
                     /translation="MKVCIISVRDVPIPYELQALNLYQIKNGSLADKAAKAFRFFDSI
                     LFIGSCAIAIKTLFRHLSDKMSDPSILVSDEKFLNTVLLCGSHMAGGQRAGEKISSAL
                     KNNFVFSTATDINGLTGIDLFCRDNCMTMEKKEILHINTELLKGKQIELSKDFSDFSL
                     PKQYVLSETPKIRLNSYNSDSVSLYTKDFSIGVGFHKNTCALELIREFQTSVPGWIRQ
                     RTGTIATIEKKYNTLVFHEFARTCNIPFTVCCETQNLDIAVRELGLKENPVVRKNTGV
                     GAVSEPCAFIASGKGKKIMEIKRKNCKFVVYQNKNIKPDKDPYNFEKVPEVFNEL"
     gene            6205..6930
                     /gene="cobJ"
                     /locus_tag="PLU28_01495"
     CDS             6205..6930
                     /gene="cobJ"
                     /locus_tag="PLU28_01495"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: protein motif:HMM:TIGR01466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030789 - precorrin-3B
                     C17-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HOG33770.1"
                     /translation="MNSKLYVVGIGPGKYELLTAQAVKTLNLSDYIVGYERYITYIQP
                     YFDKKYVSTGMTDEILRVEQAIELVKNGFNVSIVSSGDPGVYAMACLAIEKTQNTSIE
                     VTVIPGITSANSAAAVLGSPLTLDYCVISLSDLLVPWSQIKNRLECFAKSQAATVIYN
                     PRSTKRLWQLKEALEIFRTHRKKFIVAVVKNTYLEDQQVIISDSTSEDWIEKTDMMST
                     VFFCSEETIILNGMVVMPRGYLK"
     assembly_gap    7102..7201
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7202..7834
                     /locus_tag="PLU28_01500"
     CDS             <7202..7834
                     /locus_tag="PLU28_01500"
                     /EC_number="1.3.1.106"
                     /inference="COORDINATES: protein motif:HMM:NF014613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016994 - precorrin-6A reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6A/cobalt-precorrin-6A reductase"
                     /protein_id="HOG33771.1"
                     /translation="KITGQISYFFPAGKKVGVCIGKKRYFYDYIIDLSHPFALQIKQK
                     MNMLAAHKILRYVRRQTQYSNFFYGPSTMFFSLYGQGYTKVLSLLGHKGTYDFIKALK
                     NFDNPDRYLIISRSIEPVSFGVNYKFDPDLLFDRNWFDSILEKYPIQAVLIKDSGKEG
                     MTEEKIRYFSQKNKTVFMLKAPINNHAMIFDSLETLKKDILQKVMKHEKP"
     gene            7821..8831
                     /locus_tag="PLU28_01505"
     CDS             7821..8831
                     /locus_tag="PLU28_01505"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOG33772.1"
                     /translation="MKNHKLNYAVLLLILALSIIFFVGQGYIKIPFKTTFDMILGEIT
                     NNSLKEQNLKFWYTIFEVRLPRVLISGVCGAGLAFCGLIFQSVLLNPLADPYILGVSS
                     GASFGSALAIMFNLNFLGLFTTSFFAFLFSIISLVLTMAISFIDKSINKTSLILAGII
                     VNSFFSAGLSFIKYIAGDEVGTIVFWLMGSFSSRSWNDLLWVSLTFFPLLVITYFFTE
                     KMNILSLGDRNAATLGINTTRLRVVLLTLASIISSIIVSRSGIIGFVGLVVPHICRFI
                     WGSDNKKLIFYSPLIGALLMCYADNFIRLVLPAEIPIGILTSLIGAPFFAFIMRRKMR
                     RS"
     gene            8833..9615
                     /locus_tag="PLU28_01510"
     CDS             8833..9615
                     /locus_tag="PLU28_01510"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG33773.1"
                     /translation="MVIEMRNVGYSINGHEILKNISLCLQRGRFYGVLGPNGAGKSTM
                     IDILCGINRHYSGEVFIEGKDVKLTTPRELSRIISMVPQNFYINFPFSVKEVLMMGRH
                     PHKKTFTPYTHKDYEVVENIYNAFGFEKFEKTSVMEMSGGEKQRVFFAKALVQETPII
                     VLDEATSNLDIYYTHKLLSVMKQQVLSAKKTVISVFHDINTALCYCDDFIFIKDGTVT
                     ATGNMEQTMNKENLRGLYNMDFKIITQNHRAVSILPNQEEAE"
     gene            9612..10865
                     /locus_tag="PLU28_01515"
     CDS             9612..10865
                     /locus_tag="PLU28_01515"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOG33774.1"
                     /translation="MKKIALLLVSLCICLIAFANVYPYTFQDDSGNTITVRQPFKRIL
                     SLYGAHTENFYYMGAQDLLIGISDNEAFPPSFRNKPSFSYKDDPEKFIQADPDLVILR
                     PMIVQRYPKLIEKLQKYGITVVSLQPENYSDIFNYWEVLGVLCGKIQESKDMKDSFLN
                     QLNMFKELDKSAYTTVFFESVHKNFKTTTNGSIADFVLKRSGLDNIAQSAQQVREGST
                     IAEFSKEQLIDNGDKIEFYIAQVGVMNKITVQAIKKEPGIAAVKAVREDNIILVDEKI
                     ISRPTPRIIYAIMDFYKIMHNDLFMSEYELYNDAPLTNLSFSRIIIDCLKIPFTTPEY
                     FTEEKEKNGHLFGQFKDIDYVSTAHIYAETAFYNDIVNTSADRFEPDKKVSSQSIADT
                     LKKLTGKTLEKTLSTNRELIDYLRK"
     gene            10870..12144
                     /locus_tag="PLU28_01520"
     CDS             10870..12144
                     /locus_tag="PLU28_01520"
                     /inference="COORDINATES: protein motif:HMM:NF002204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HOG33775.1"
                     /translation="MQKVFFVTSLFTSSGKTLFTLGLLNSLKESVVSFKTGPDFIDPL
                     FHKKITEKKGYNLDSFFLDGPELKKHFLTAAAGYPNIVIEGAMGVLDGIKKGYASCDD
                     VADQLGLPVILLIESYPSIHTMGAVIKGVMSSMKSSVSAVIITKSKSQKLFDLQKEDI
                     LNSTGIEKIFRFPYDEALTVKSRHLGLDTSCNEIVKIAESSARIIKENMDLSCLCTEI
                     PELSQTVLPESTYEKTVAIASDEAFLFFYHENIEFFQKCGYRPVFFSPIKDSIPPDAD
                     MYYLPGGYPELYAKQLSANKPMLRSLYELCTSEKPVLAECGGFMYLGNSIQEYPMVGF
                     FDVSSSMKPSMQGNFGYQNIKIKNSDHTYKGHEFHYAQMSYGEKPNALKITMESSKKT
                     SVEGLLKQKTLGSFTHFYFRSSKKDDALWLYL"
     gene            12129..13547
                     /locus_tag="PLU28_01525"
     CDS             12129..13547
                     /locus_tag="PLU28_01525"
                     /inference="COORDINATES: protein motif:HMM:NF001989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HOG33776.1"
                     /translation="MAIPIMVTGTSSGAGKTLVCACLCNILSKLGYSVAPFKMQNMSL
                     NSIVSPLGGEMSIAQYIQAVACKKTPCVKYNPILLKPQDHMTYVVFNGEYYAKYSPQE
                     YMKSRKSEFFEKGIEILKELMQQNDYVIIEGAGSPAEVNLMKYDITNMAVAKAVKAHT
                     ILVADIERGGSFAGIVGTMEIFNNEERELVKGFIFNKYYGDKSMLDDGIDYLYDRYKI
                     KTVGIIPYKKHSIPEEDSLITWESRTGDLDIRIVKYPHISNFSDFEPLQKKNGVKYIS
                     TAQEVSGDLIILPGSKSTVSDLLWLNETGISQKLKDAAEEGCFILGICGGFQMLTQSI
                     DDTFESNSQTPGLGLIPLKTHFEERKITESVKGKVFFNGRSFDVTGYELRHGRTECAE
                     NWLIDFDSHKDGFFKDNVMGTYLHGLFYNHDFTNFFLNYLRILKGEEEKDFGNYSLYD
                     EISSFSDYVSEHIDMKEILQWR"
     gene            13538..14449
                     /gene="cbiB"
                     /locus_tag="PLU28_01530"
     CDS             13538..14449
                     /gene="cbiB"
                     /locus_tag="PLU28_01530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016880 - acid-ammonia (or amide) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HOG33777.1"
                     /translation="MALIIAVIADCLFGEPYLVFHIVHYIGAVVDFLDKRLKHTIMNG
                     MLTYVLTCSIFLFGTFLLLHTGSLLTAVFHVFLLKSCFAISSLYVHVGRCRQDDTVGL
                     RKAVSMIVSRDTTNLSKGELYSAAVETLAENYVDSVLSPIFFYLIFGIYGVVIYRVTN
                     TMDAMIGYRNEKYEKFGKFAARADDVLNYIPARLSVLFFLVFSPVAVSKSVFKFGGIK
                     INGTKPMASMSGLLKINLKKNGVYDLNPQYRQPEKKDLDRAMLYYILIVTMTCIIGAV
                     CIWMIQNFSWLNMEETFLFRGLIFQYL"
     gene            14371..15324
                     /locus_tag="PLU28_01535"
     CDS             14371..15324
                     /locus_tag="PLU28_01535"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOG33778.1"
                     /translation="MDDSKFFMAEHGGNIPLSWTDFSISVNPYYPDFFRNYENELSAL
                     SQRYIYFEDIEKNLGSIVGHEVFLTAGTTETLYVLNMAFKNNIVVENGSYSEYERTAQ
                     LFGKKVFKVDSVVESNFKDCLVFLNNPKNPTGRFYTYPELKKFTEQTVKNNSIPVIDD
                     AFMDFIPDIPEWEKNWVFNGTIHMRTYTKSYGLAGIRTGYFIDPYKKMHRFRPPWSIG
                     SIGRLFIQKLILDDGKFLKASMQKLNLERQRLNELLGTKFISPFFLIKVKEKTELLEK
                     LAQNHMMVRDAYSFGLKDHIRFSVKTPEENNRLIRILTAYI"
     gene            complement(15350..16513)
                     /locus_tag="PLU28_01540"
     CDS             complement(15350..16513)
                     /locus_tag="PLU28_01540"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018455 - alcohol dehydrogenase [NAD(P)+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOG33779.1"
                     /translation="MENFVFSNPTCLIFGKDTIKRIGKEIKKRGVKNVLVLYGSGSIF
                     KNGVYDAAVKSLKDNEVGFVQLGGIKPNPVLSKVYEGIKTAMKEKTDGILAIGGGSVI
                     DTAKAIAAGIGYDGDVWDLFEGKAPVKTNVPIFTILTISATGSEMNNGAVITNEGENK
                     KWSIGSPDLFPKASIIDPSVQFSLPWNQTANGAVDAITHVLELYFDKTENNMIMDEYS
                     HAIVKTVLKSTDLLMKNPSDYDARANLALSATLALNKTNSVGRQGGDWSSHWIEHSLS
                     AFYDVAHGAGLAIVMPAWMDYVYAECIHKWVVFAESIFGITAGTPDEKAKAGIAELRK
                     FYKKIQQPTKLSEINVPYEDLPKIAANASMKGKIGNIKRLDQNDILNILKLAY"
     gene            16679..17692
                     /gene="cobT"
                     /locus_tag="PLU28_01545"
     CDS             16679..17692
                     /gene="cobT"
                     /locus_tag="PLU28_01545"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: protein motif:HMM:NF000996.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HOG33780.1"
                     /translation="MLLNEKVQKKIDMLTKPKGSLGLLEKIALKYALIQGSSTVSLPK
                     DKRVYVFAGDHGVVEQGVSAYPKEVTAQMVFNFLAGGAAINVFSKHNNTSVYIVDAGV
                     DYDFSSEPRVINKKVGRSTKDFSSGPAMTSEQAELCIKHGREVATQAINDGADLLAIG
                     DMGIGNTTTAAAICAGLGIPVEEVLDVGTLIDNQMLTKKGEVLEKTMKLHSPFKSPMD
                     VLMKAGGFCIGEMAGFMLECADRKVPFVVDGFPVSSAALLAYRINPRIRDYMFIGHQS
                     AVKSHLPIIKELGLEPILKLEMRLGEGTGAVLSFNIIEAALKMLNEMSSFEDANISKS
                     DIE"
     gene            17689..18174
                     /gene="cobU"
                     /locus_tag="PLU28_01550"
     CDS             17689..18174
                     /gene="cobU"
                     /locus_tag="PLU28_01550"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: protein motif:HMM:NF014351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HOG33781.1"
                     /translation="MILITGGIKSGKSSYALELVQKYPRKAFIATAEPFDEEMKQRIK
                     KHKQERDSSYDTYEEPLEVASLLPKLTDYDVILVECITTWLSNLLYYEKDIEKYTESF
                     MNNLTGKEVIITNEVGLGIVPIDPLSRRYTDAIGKLNSKLARKADEVYLMVSSIPVKI
                     K"
     gene            18171..18767
                     /gene="cbiR"
                     /locus_tag="PLU28_01555"
     CDS             18171..18767
                     /gene="cbiR"
                     /locus_tag="PLU28_01555"
                     /inference="COORDINATES: protein motif:HMM:NF041277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobamide remodeling phosphodiesterase CbiR"
                     /protein_id="HOG33782.1"
                     /translation="MIGSTSWVVPGTYYENAVILQKYVDFIELLVFTWDEETKKLFDK
                     ELKHLKKLNIRYSVHLPTDTAENAAKVIEYFSLNDFELLNYVLHPMPGWQKLEKLPGV
                     SVENLIGTLEYSEKMTFDIGHHILGKKFDAAYKTNIVEYHLMGVENSTDHIPLNDYAI
                     DTLKEFYLPGRLCCFEVFELEGLLSSLRRWKEYEKNSQ"
     gene            18748..18969
                     /locus_tag="PLU28_01560"
     CDS             18748..18969
                     /locus_tag="PLU28_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG33783.1"
                     /translation="MKKILNEIRMTVSFLSRIPAGGTGEIKNIPRYFTIVGYLSGMIY
                     MLLTHTLGKGVFQTVLTMSRFFLLRPFPL"
BASE COUNT         6189 a         3802 c         3594 g         5346 t
ORIGIN      
        1 ccctcacaaa ctccattatc tgtttaggaa tatccgggtc tattataaca tcagaagtta
       61 taaagtatcc tattccgtgt ttggcctcca ctatcttttc aaactggagg tactccagtg
      121 cacgcattat ggtattcata ttcacaccga aaatattttc catttctctc accggcggta
      181 gctggcttcc gtctgtaaac cttccgagaa atatctcttt ttttatctga ttcattatct
      241 gaagataggc aggaacaccg ctatgcttat ctatctttgt tagcatacag atctcctcct
      301 aatacaacgt gctcaagaca gtgactccgt cctgagttct tatatcgagt gttccttcat
      361 tgtttctggg ctgcattact tctatccttc cggagtttga acttatacta agaatacgga
      421 caccctccca gctgtcagta tactttattc ttccgttggc gtaaaggctg tctatattaa
      481 atgtttttac ctcagaaaca ttaagatcca gttcgcatat gtttccgttt attatcattt
      541 tttcagcttt taccattccc tttgccttaa gatcatcagc aattaaaata acagtttttt
      601 ctgcagtaag tgtagcgtct atggtacaca cctgagtgtc cgcactcacc ctctgtgcat
      661 aaaactctcc actgatgctt acagcggagt tttgtatatc aagaatctct atccgttcac
      721 cacttggcaa tgaaatagtt gctgcttcag acgtgcttct gacatagtcc agagcactta
      781 tattattgac atcgcctttc tttttaaatg caacctttcc tatggcacgc ggattatttt
      841 catcagaagt gaattttatg ttcaaagccc caccgctcac cctgtaactc aaagcaggca
      901 acaacgcacc gtcctctact acggtgtagc cttcatgaga atactcggac tcccagtccg
      961 aaaagtaaat atacctccat gaagagctgt taaaaaacct gctcaccaga ttataatccc
     1021 tggatatagc aaggaacagc accggaatga ttacaaaaat aaccgcaagt actatgaaaa
     1081 gcgttgagaa ccttttaatg ttcatcgcat acaaaaaaat tgcaacactg gcaagaataa
     1141 tcttcagcgg cacaaacggc accagaaccg ctgcgagcga tataactata acaataagga
     1201 atctcgcaag cttttcgtat tctttcaaca caagtacacc tccaataact ttagtgttct
     1261 ataaaattat aacactaaaa cacatcaatg tcaacctgaa tgcttatacg catttaatcc
     1321 tcaaataata agcactttca gccttattaa tatatccgga tatattctct ttgacattgc
     1381 cttttttttc tgctaaaatt taaaatagaa aattaaatat gcattcttaa ggtgaataac
     1441 agggaatcgg gtgaaaaacc cgagcggtcc agccaccgta atcagggacg aacatcacat
     1501 atgccaccgg gcttttccgg gaaggcgtga taagtaggag gatctgagag ccgggagacc
     1561 taccttaaga tttgttggct ttgctttctc ttggaataga aagcagatat tttgtttttg
     1621 gaggcagaaa tgctggagta tataaaagat cctgcaatga ttgagagaaa aagctttgaa
     1681 ataatagcag aaaaactgga tggaaaactt tcccatttag gcagtataga acaaaacatc
     1741 ataaaaagag ttgtccattg ctctggaaat cttgatttgt gggataagct gaacttctca
     1801 gaagattttt ttgaatcttt tctttcattt ttaagataca aaaccgttta tacagacgta
     1861 aatatggtaa aaagtgggat aaacaaaaaa attattcccg agcttcaagt atactgcttc
     1921 atagatgagc ccgaaatagc tgagctctca aaagaacaaa aaatttccag ggcatatctt
     1981 tcaatgcaaa aagcacttcg tgcaaatccg ggcatcttcg ttatcggtaa tgctccaact
     2041 gctcttttta aactgcttga aagtacagaa aaacttttta taataggcgt tccggtaggt
     2101 ttcgtcggtg ctgcccagag caaagaactg cttgttgggt ctgttcattc atatgtaacc
     2161 ctatccggta cattcggagg ctcaaatatt gcagctgcta taatgaatgc actgcttaaa
     2221 gaggcttaca atgcagcaat acgttaacgt taacggacaa ctgctcagac gtggctttac
     2281 aacagggaca tgtgcgtgtg cggcacttaa agcctgtatc ctatggactc tttacgataa
     2341 aaaagatccc gaagtctctg taagacttaa cgaaggcagt ctcgttaatc tgaaaataga
     2401 aagttatgaa ataatggaag gcaaaaagta tgctttcata agaaaagatg ccggtgatga
     2461 ttcagacatt acaaatcact gtctgatata tgtatcttat aaagctacag agcccggcac
     2521 tggttttgtc ttcacttcag cagagggcgt cggactggta accaaaaaag gtttggacgt
     2581 cagacaagga gagtacgcca tcaatccagg cccacggaag atgatgaaat cagttttaca
     2641 ggaatacggt ataaatgacg ctataataac cgttaatgtc aaagatgggc aaaaaattgc
     2701 cataaaaact ctcaactcct cactgggaat agtaggagga gtatcgatcc ttggaacaag
     2761 cggaatcgtt gaacccatga gtgaagaggc gtggagagaa tcgttggttc cccagataag
     2821 aatgattggc tcatacttta gcagcataac acttctaagc ggtgggaaaa gccggaagat
     2881 ttatgaaact tctacgttct tcaaaaatga ttttcttctt tgtggtaact tttttgggtt
     2941 ttcaatagaa aaggctattg aaaaccatat aaaaactatc cacattatgg ggagcttcca
     3001 aaaaataatc aagctggcag ggctaaacct caataccgac agcaggcttt ccgacagtaa
     3061 aaatgaactt ttagccctaa tgtacataat gtttaaaaaa acctacgatg aagaaatggt
     3121 caaaaaaatc ctttctttga atccggttta cagcataatt gaactatttg atgaacaccc
     3181 cggtgaaagc aaggcttttt ttgaattttg tggttcatac atagctcaaa agctcgagaa
     3241 aagattcaat caacctttta taataacatt gtgcgaaaag gaaagtatac tttcgcatac
     3301 gggaggctta ctctatgctt tacataatgg gaacacagat taactgtgaa gagaatttta
     3361 caaccagaca gcttgatatt atttcaaagg ctgatttttt tgccggcttt tcattctctc
     3421 ttcagatcgc aaaaaaagta aatccgactg cagagtttta ctgcatgaac atttatgact
     3481 cgctaaaaga agaaaatata gatcatctgg tatccattct cagaaaatat aaaaatccgg
     3541 tcattatggt ttctggcaac cccttgatgt tttcctacac taaaaacatt acgtccaagc
     3601 ttaatcaaga tgagtatgaa gttctcgttt ctccatcaag cgtggattat ctcgcccaga
     3661 aaaccaagac aattctgaac ggcttatgct atgtgagcgg ccataactca cacaatctcc
     3721 aaaaaacata taatgaagta cttttttttc ttaatatggg cagacctgtt gcctacttcg
     3781 tcaaaaacat ggacgatttc tcaaaccttt ccacgcttct tggaaatgtt aaagtaacca
     3841 ttgcggcagg ttttgaactt ggaactccac aggaaaaaat ttttaacttt gaaagtcaga
     3901 actttcctga ttttatacac ggaagatgga ttctcatcct ccagcctgca caaaaagatt
     3961 ttcttgacag ttttccgaaa aactcagact taaactgcaa caacatacct gtatcacgtg
     4021 agtggacaag atttttgtgt gtaaactccc ttaatcgcca cgataagcac gatatcatct
     4081 gggatctcgg agccggaagc ggcgccacat caatatatat ctcttctttc tacgggcgta
     4141 actgttcggt aaaagctata gaaaaagaga aaaacagatt cggacatctt gttgaaaata
     4201 ccgccatgaa tcctgaaatc ttctgtataa acaaagattt taagaacata ctcagaaatc
     4261 ttgacagacc tgatatagtc catcttggag gaggcataga cagagaaagc ctctcactta
     4321 tttctcagta catgcccaaa ggcagtattc tgatctccag cgctacaacc caacagagtc
     4381 tttgcacagt tctgcaaagt ccgggatatg cttttgagta ctccatgtat gcaaaattta
     4441 gttcaaacaa catatcaaac aaggatgctt tcaaaggcga gtatgttttt tattgtatac
     4501 ggggtgaaaa aattgattag ttttatcgga tgcggtccgg gaagcaaaga ttttcttacc
     4561 ataaaaggct atgaaaggat caaaaccgcc gatgttatta tttatgctgg ttccacggta
     4621 gaccacctgc ttacggatat atcgggggct ggaaaaatat ttgactcttc atctatgacc
     4681 ttggaagata tagaaaaggt ttttttagat aatagaagtc tcaacattgc aaggatgcac
     4741 tccggagata catccctata ctctgccata gacgaagaga tagccctggc tgagaaagca
     4801 aatatagcct tcgaggttat accgggtata agcgcatact cttattttgc atccaaactt
     4861 ggaattgaac ttacttcacc aggaatatgc caatcggttg tgcttacacg actaaatggc
     4921 agaactccgg taccggaaaa catagaaagt ttttttgggg aacagccctc atgtgcaatc
     4981 tatctttcag gttctaagcc atatgaagta ctttcgcttc tgaagaaaca ctacgacaag
     5041 aacgccatgc tggccatagg cacacacctt gcacgcgagc aagaaagaat tgaggtgaaa
     5101 cctttgcata gctggcatga cattgatttt ccttccaatc tgactctttt tttaattttc
     5161 aggaaaggaa gcataaaatc ctgtctgtac gataaaaatt tcagtcatgg aatgagaaaa
     5221 tgaaagtctg cataatatcg gtaagagatg tacctattcc ttacgaactt caggcactta
     5281 acctatacca gataaaaaac ggttcccttg cagacaaggc cgcaaaagct tttcgttttt
     5341 ttgacagcat actgtttata ggaagctgtg cgatagctat aaaaactctt ttcagacatc
     5401 tttcagataa aatgtctgac ccaagtattc tagtgtcaga tgaaaaattc cttaataccg
     5461 ttcttctctg cggttcacac atggctggcg gacagagggc cggagaaaaa atatcttctg
     5521 cgcttaaaaa caactttgtc ttttccacag ccacagacat aaacggactt acaggaatag
     5581 atcttttctg tcgggataac tgtatgacta tggaaaaaaa agagattctg cacataaaca
     5641 cagaactgct caaaggaaaa cagatcgagc tttcaaaaga tttttcggat ttcagtcttc
     5701 cgaaacagta cgtgttgtcc gagactccaa aaatacgtct taactcatac aactcggatt
     5761 cggtatcgct ctatactaaa gacttttcaa tcggtgtagg ctttcacaaa aacacatgtg
     5821 cactggaact tatacgagag ttccagacct cagttcccgg ttggattaga caaagaacag
     5881 ggacaatagc aaccatagaa aaaaagtata ataccctggt cttccatgaa tttgcaagaa
     5941 cctgcaacat ccccttcaca gtctgctgtg agacacaaaa ccttgatata gcggtaagag
     6001 agcttggact caaagaaaat ccagttgtac gtaaaaacac aggtgttgga gcggtatctg
     6061 aaccatgtgc atttatagct tctggaaaag gaaaaaaaat aatggaaata aaaagaaaaa
     6121 actgcaagtt tgtagtatac caaaataaaa atatcaaacc agataaagac ccttataatt
     6181 ttgaaaaagt tccggaggtg tttaatgaac tctaaacttt atgtggtcgg aatcggaccg
     6241 ggaaagtatg agttgctgac agcccaggcg gtaaaaactc tgaacctgag cgattatata
     6301 gttggatatg aaagatatat aacgtatatt cagccctatt ttgataaaaa gtacgtctca
     6361 accggaatga ccgatgaaat acttcgtgtt gaacaggcta tagaacttgt aaaaaacgga
     6421 tttaacgtaa gtattgtttc aagcggtgat ccgggagttt atgcaatggc ctgccttgcc
     6481 attgaaaaaa cccaaaacac cagcatagag gttaccgtga tccccggcat aacctcagct
     6541 aactccgccg ctgccgtact gggctctccg cttacacttg attattgtgt gataagtcta
     6601 agtgatctgc tggtaccatg gtcacaaata aagaacagac tggaatgctt tgctaaaagt
     6661 caggctgcta cggttattta taatcccaga agcaccaaac gtctctggca gttgaaagag
     6721 gctcttgaaa ttttcagaac acaccgaaaa aaattcatag tggccgtagt gaaaaatact
     6781 tatcttgaag accagcaagt aattatatct gacagcacat ctgaagattg gattgaaaaa
     6841 acggatatga tgagcactgt ctttttttgc agcgaagaga ccattatctt aaacggaatg
     6901 gttgtcatgc ccagaggcta tttaaaatga agttaggagt aatcgcagga acatcttcct
     6961 gtcgaagtat ttccgacttt ttagaaaatc agaacataga gtattcagta catcttaaaa
     7021 aaggatatct cttttcagaa tgctccaaaa taaccggaca gatatcgtat ttttttcctg
     7081 ccggaaagaa ggtaggagta tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7201 naaaataacc ggacagatat cgtatttttt tcctgccgga aagaaggtag gagtatgcat
     7261 cgggaaaaaa agatactttt acgattatat tattgatctg tcccatccat ttgctttgca
     7321 gataaaacaa aagatgaaca tgcttgccgc acataaaata ctaaggtatg taagaaggca
     7381 gactcagtat tcaaactttt tttatggacc atctaccatg tttttctctt tatacggaca
     7441 agggtatacc aaagttctga gtcttctggg acacaaaggt acctatgatt ttataaaagc
     7501 actgaaaaat tttgataatc ccgacagata tctcataata agcagaagca ttgagcctgt
     7561 ttcgtttggt gtaaactaca agtttgatcc ggatcttctt tttgatagaa actggtttga
     7621 cagcattctg gaaaaatatc ctattcaggc ggttcttata aaagattcgg gaaaagaagg
     7681 catgaccgaa gaaaaaataa gatacttttc gcagaaaaat aaaactgtct ttatgcttaa
     7741 agcacctata aacaatcatg ccatgatctt tgactctctt gaaacactta aaaaagatat
     7801 ccttcaaaag gtgatgaaac atgaaaaacc ataaactgaa ctatgctgtt cttcttttga
     7861 ttttggcact ttcaattatt ttttttgtag gtcagggata tataaagatt ccgtttaaaa
     7921 caacctttga catgatactt ggagaaatta ccaacaactc gttgaaagaa cagaacctta
     7981 agttttggta caccattttt gaagtccgcc ttccgagagt tcttatctca ggggtatgcg
     8041 gagccggact tgcattctgc ggactaatat ttcagtcggt gcttcttaac ccacttgctg
     8101 atccttatat actaggtgtt tcatcaggag catctttcgg ttctgccctt gccataatgt
     8161 tcaatcttaa ttttttaggt cttttcacca catcattttt tgcttttcta ttttctatta
     8221 ttagtctggt acttactatg gcaatatcct ttattgacaa aagtataaac aaaacatcgc
     8281 ttatactggc ggggataata gtaaactctt ttttctcggc aggtctgagc tttataaagt
     8341 atattgccgg cgatgaagta ggaaccattg ttttctggct tatgggttct ttttcttctc
     8401 gatcgtggaa cgatctgctc tgggtaagtc tgactttttt tcctttgctt gtaataacat
     8461 atttttttac ggaaaaaatg aatattcttt cacttggaga cagaaacgct gctaccttgg
     8521 gcattaatac caccaggctg agagttgtgc ttcttacact cgcaagcatc ataagctcca
     8581 taattgtttc aagaagcgga attataggat ttgtaggtct tgttgtacca cacatatgca
     8641 ggttcatatg gggctctgac aacaaaaagc ttatctttta ctctcccctt atcggagcac
     8701 ttctgatgtg ctatgcagac aactttatac gtctggttct tcctgctgag attcccatag
     8761 gtattcttac ttctcttata ggagcacctt tttttgcttt cataatgcgc agaaaaatga
     8821 ggaggagctg atatggttat cgaaatgaga aatgtaggat acagcataaa cggtcatgag
     8881 atacttaaga atatatctct ttgtttacaa agaggaaggt tttacggagt actgggccca
     8941 aacggagccg gaaaaagcac catgatagac attttatgcg gaataaacag acactacagc
     9001 ggcgaagtct ttattgaagg aaaagatgta aaactcacta ctccaagaga gctgtcaaga
     9061 ataatatcaa tggtgccaca aaatttttat ataaactttc ctttttcagt aaaagaagtc
     9121 ttaatgatgg gaagacatcc ccataaaaaa acttttactc cctatacaca taaagattac
     9181 gaagtagtag aaaatatcta caatgccttt ggttttgaaa agtttgaaaa aacatcggta
     9241 atggagatga gcggcggtga aaagcaaaga gtattttttg ccaaagctct tgtacaggaa
     9301 actccaataa tagttttaga cgaagccaca tctaaccttg acatctacta cactcacaag
     9361 cttctgtcag ttatgaagca gcaggtgctt tcggcaaaaa aaacagttat atcggtattc
     9421 cacgacatta atacagcttt atgctactgc gatgacttta tcttcataaa ggacggaaca
     9481 gtcactgcca ccggaaacat ggaacaaacc atgaataagg aaaacctgag gggactttat
     9541 aatatggatt ttaaaataat aacccaaaat cacagggcag tatcaatact gccaaatcag
     9601 gaggaagcag aatgaaaaag attgctttgt tgcttgtatc actctgtata tgtctcattg
     9661 catttgccaa tgtttatccg tacaccttcc aagatgactc aggaaataca ataaccgtaa
     9721 ggcaaccgtt taaaaggatt ctgtctttat atggagccca tactgaaaac ttctactaca
     9781 tgggagccca agacctgctt ataggtattt cagacaacga ggcatttccg ccatcattca
     9841 ggaacaaacc atcttttagc tacaaagatg atccggaaaa gtttattcag gcagatcctg
     9901 atcttgtaat actaagaccc atgatagttc agagataccc aaaactcatc gaaaaacttc
     9961 aaaagtatgg aattacagta gtatctttac agcctgaaaa ctactctgac atcttcaact
    10021 attgggaagt tcttggagtc ctatgtggaa agattcagga aagcaaagat atgaaagaca
    10081 gctttctgaa ccagctaaac atgtttaaag agcttgataa atcagcatac acaactgttt
    10141 tttttgaatc tgtccataaa aacttcaaga ctaccaccaa cggatcaata gccgattttg
    10201 tccttaaaag atcaggactt gacaacattg cccagtctgc ccagcaggtc agagaaggct
    10261 ctacaatcgc agaattttca aaagaacagc ttatcgataa cggagataaa atagagtttt
    10321 atattgccca ggttggggtt atgaacaaaa taaccgtcca ggctataaaa aaagaacccg
    10381 gaattgctgc agtaaaagca gtaagagaag ataatataat actcgttgat gaaaagataa
    10441 tatcaagacc tacaccaaga ataatatatg ccataatgga cttttacaag ataatgcaca
    10501 atgatctttt catgtcagag tatgagcttt acaatgatgc tcctttaact aacctctctt
    10561 tttcaagaat tattatagac tgccttaaga taccctttac aacacctgaa tactttaccg
    10621 aagaaaaaga gaagaacgga catcttttcg gacagttcaa agatatagat tatgtaagta
    10681 cagctcatat atatgcagaa acggcttttt acaacgatat cgtcaataca tctgcggaca
    10741 ggtttgaacc tgacaaaaaa gtatcttcac aatccatagc cgacacactc aaaaaactca
    10801 caggaaaaac attggaaaag acattaagta ccaacagaga acttattgat tacctgagaa
    10861 aataatagta tgcagaaagt tttttttgta acctctctct ttacatcaag cggaaaaaca
    10921 ctttttactt taggacttct taacagccta aaagaaagtg tggtctcttt taagacaggt
    10981 cccgatttta tagatccttt atttcataaa aaaatcacag aaaaaaaagg atataacctt
    11041 gactcttttt tccttgatgg acctgaactt aaaaaacatt ttttaacggc agcagccgga
    11101 taccctaaca tagtaataga aggggctatg ggcgtattgg atggaataaa aaaaggatac
    11161 gcttcgtgcg atgatgtggc agaccaactt ggtctgccgg ttattctgct tattgaaagc
    11221 tatccctcta ttcacactat gggtgctgta ataaaaggcg ttatgtccag tatgaaaagt
    11281 tcagtatccg ctgttattat aaccaaaagc aagtcccaaa agctttttga cttacagaaa
    11341 gaagacattt taaacagtac cggaatagaa aagatcttca ggtttccata cgatgaagct
    11401 ctcaccgtaa aaagcagaca tctcggactt gacaccagct gcaatgaaat cgttaagatc
    11461 gcagaatcat ctgcacgtat aataaaagaa aatatggatc tttcctgtct gtgcacagaa
    11521 attcctgaac tttctcaaac agttcttccg gagagtacat atgaaaaaac tgttgccata
    11581 gcttcagatg aagctttttt attcttttac catgaaaata tagagttttt ccaaaaatgt
    11641 ggctatagac ctgtgttttt ttcacctata aaggacagca tccctccgga tgccgatatg
    11701 tactacttac ccggaggata ccctgagctt tatgcaaaac aactttccgc caacaaaccc
    11761 atgctccgct ctttatatga actctgtaca tcagaaaaac ccgtactggc agaatgcgga
    11821 ggctttatgt atctgggaaa cagtatccag gagtatccta tggtaggttt ttttgatgtt
    11881 tccagcagca tgaagccctc tatgcaggga aacttcggct atcaaaacat aaaaatcaaa
    11941 aattctgatc acacctataa aggacatgaa tttcactatg cacagatgtc atacggtgaa
    12001 aaaccaaatg ctttaaaaat cactatggaa tcttcaaaaa agacttcggt ggaaggactg
    12061 ctaaaacaaa agactctggg cagctttaca cacttttact ttagatcttc aaaaaaggat
    12121 gatgcattat ggctatacct ataatggtta cgggaacctc ttctggcgcc ggaaaaactc
    12181 ttgtgtgcgc ctgtctatgc aatatacttt caaagctggg atacagcgtt gcccctttta
    12241 aaatgcagaa tatgtctctt aactcaatcg tctcacctct tggaggagag atgagcatcg
    12301 cccagtatat ccaggcagtt gcatgtaaaa aaactccatg cgtaaagtat aatcctattc
    12361 ttctcaagcc acaggatcac atgacctatg tggtattcaa cggggagtac tacgccaaat
    12421 actctcccca ggagtatatg aagagcagaa agtcggagtt ttttgaaaag ggcattgaaa
    12481 tactgaaaga acttatgcag caaaatgatt atgtaataat agaaggcgcc ggcagtccgg
    12541 cagaagtgaa cctcatgaag tacgatatta ccaacatggc ggtagcaaaa gctgtgaaag
    12601 cacataccat actcgtagct gacattgaaa gaggaggctc ttttgccggc atagtaggaa
    12661 caatggagat tttcaataat gaagaaagag aactggtaaa aggctttatc ttcaacaagt
    12721 actatggtga caaatcaatg ctggacgacg gtatcgacta cctatacgac cggtataaaa
    12781 tcaaaacagt cggtataatt ccctacaaaa aacactccat acccgaagaa gattcgctta
    12841 taacctggga aagcagaacg ggcgatcttg atatacggat agttaagtat ccacatatct
    12901 cgaacttttc tgattttgaa cccctgcaga aaaaaaacgg agtaaagtat atttcaactg
    12961 cccaagaggt ttcaggcgat ttgataatac ttcctggctc taaatcaacc gtcagcgacc
    13021 ttttatggct taacgaaaca ggaatatctc aaaagttaaa agatgcggct gaagaaggct
    13081 gttttatcct tggaatatgc ggaggctttc agatgcttac ccaaagtata gatgatacct
    13141 ttgaaagcaa cagtcaaacc ccgggacttg gacttatacc tttgaaaaca cactttgaag
    13201 aaagaaaaat caccgaaagc gttaaaggga aagttttttt caacggaaga tccttcgatg
    13261 tcaccggata cgaactgcgg cacggaagaa ccgaatgcgc tgaaaactgg cttatagact
    13321 ttgacagtca taaagacggc ttttttaaag ataacgttat gggtacttat ctgcacggac
    13381 ttttctacaa ccatgacttc acaaactttt ttctgaacta tttacgcata ttaaaaggag
    13441 aagaagaaaa agattttgga aattactcat tatacgacga gataagcagc ttttcagatt
    13501 atgtatccga acatattgat atgaaggaga ttttacaatg gcgctgataa ttgctgtaat
    13561 tgccgactgc ctttttggag agccttatct ggtattccat atcgtccatt acatcggagc
    13621 agtggttgat tttctggaca aaagactcaa acacacaata atgaatggaa tgctcacata
    13681 tgtgctgact tgctccatat tccttttcgg tacattcctg ctgctgcaca caggctcttt
    13741 acttacggca gttttccatg tgttccttct gaaatcatgc tttgctatct cctcacttta
    13801 cgttcatgtt ggaagatgcc gtcaagacga taccgttggt ctcagaaaag ccgtttcaat
    13861 gatcgtaagc agagacacca ccaacctttc aaaaggcgaa ctgtactccg ccgcagttga
    13921 aactcttgca gaaaattacg ttgattcggt gctttctcct atctttttct atttaatttt
    13981 cggaatatac ggcgtggtta tttaccgtgt caccaatacc atggacgcca tgataggcta
    14041 ccgaaacgaa aagtatgaaa agttcggaaa gtttgccgca agagccgacg atgttctgaa
    14101 ctatatcccc gcacgcctga gtgttctgtt tttccttgtt ttttcaccag ttgcagtttc
    14161 aaaaagcgtt tttaaattcg gcggaataaa aataaacggg accaagccca tggcaagtat
    14221 gtccggactt cttaagatta atcttaaaaa gaacggagtt tatgacctca acccgcagta
    14281 cagacagcct gaaaaaaagg atctggacag ggcaatgctt tactatatct taattgtcac
    14341 gatgacatgt attataggag ctgtgtgcat atggatgatt caaaattttt catggctgaa
    14401 catggaggaa acattcctct ttcgtggact gatttttcaa tatctgtaaa cccttactac
    14461 cccgactttt tcagaaacta tgaaaatgaa ctgagtgctc tttcacaaag atacatctac
    14521 tttgaagata ttgaaaaaaa ccttggaagc atcgtcggac atgaagtgtt tcttactgca
    14581 ggaactactg aaacacttta cgttctcaat atggcattta aaaacaatat tgtagtcgag
    14641 aatggctcgt actcagagta tgaaagaacg gctcagcttt tcggaaaaaa agtttttaaa
    14701 gttgattctg ttgttgaaag caacttcaaa gactgcttgg tctttttgaa caacccgaaa
    14761 aatccgaccg gaagatttta tacttatcct gaactaaaaa aatttaccga acagaccgtt
    14821 aaaaacaaca gcatccctgt tatagacgat gcttttatgg acttcatacc cgatatacct
    14881 gaatgggaaa aaaactgggt tttcaacgga accatacata tgcgaacata caccaaatca
    14941 tatggacttg caggaataag aacaggttac tttatagacc cgtataaaaa gatgcacagg
    15001 ttcagacctc catggagcat aggctcaatc ggcagattat ttatacaaaa acttatactt
    15061 gatgacggaa agtttcttaa ggcatccatg caaaaactta acctcgaaag acaaagactg
    15121 aatgagcttc ttggaactaa gttcatatca ccgttctttc ttataaaagt taaagaaaaa
    15181 actgaactcc ttgaaaagtt ggcacaaaac catatgatgg taagagatgc ctactcattc
    15241 ggactgaaag accatataag gttttcggta aaaacaccgg aggaaaacaa ccgactgatt
    15301 cggatactta ctgcatatat ataaaaaatt cccggcctgg ccgggaatct cagtaagcta
    15361 acttaagaat attaagaata tcgttttgat caagacgctt tatattgcct attttaccct
    15421 tcatagaagc attagcggct atcttgggca gatcctcgta aggaacattg atttcactga
    15481 gcttggtcgg ctgctgaatc tttttataga actttcttag ctcagctatt cctgcttttg
    15541 ctttttcatc aggagtacct gcggttatac caaaaatact ttcagcaaat acaacccatt
    15601 tgtgaataca ttccgcataa acatagtcca tccaagctgg cattacaata gcaagaccgg
    15661 caccgtgggc aacatcataa aaagcactca aagagtgttc aatccaatgt gatgaccagt
    15721 ctccgccctg tcttcccaca gagttggttt tattcagagc aagagtggca ctaagagcaa
    15781 gattagccct tgcatcataa tcggaaggat ttttcatgag caaatcggta gattttaaaa
    15841 ccgtctttac aatagcatgc gagtattcat ccattatcat attgttttca gtcttatcaa
    15901 aatacaattc aagaacatga gtaatagcgt ccacggcacc gttggccgtc tgattccacg
    15961 gaagagaaaa ctgaactgaa ggatcgatta tcgaagcctt tggaaacaaa tcgggagaac
    16021 ctatagacca ttttttattt tcaccttcat tggttattac cgctccgttg ttcatctccg
    16081 aaccggttgc cgatatagtc agtatggtaa agataggaac attggttttg acaggtgcct
    16141 ttccctcaaa aagatcccat acatcgccgt cataacctat acctgcggcg atagctttgg
    16201 cagtatcaat aacacttccg ccgccaattg caagaatgcc gtctgttttc tctttcatgg
    16261 ctgtttttat accctcatat acctttgaaa gtacaggatt aggctttata cctcctagct
    16321 gaacaaaacc gacctcatta tccttaagag actttaccgc agcatcgtac acaccgtttt
    16381 taaatataga accggaacca tagagtacaa gcacattttt gacaccacgc ttctttattt
    16441 ccttgcctat acgctttatg gtatccttac cgaagataag acatgtcgga ttactgaaca
    16501 caaaattttc cattgaatca ccccactgta tacgctttta aaagcagatt atattattag
    16561 aaagattata ccacttaagt acgctattag aatcatttcc ataaattatt gacatcaatg
    16621 gttataagct atatactaaa agacagacta cacttaaaat tatatgagga ggcaaaaaat
    16681 gctgcttaac gaaaaagtac agaaaaagat tgatatgcta accaaaccga aaggaagtct
    16741 tggactgctg gaaaaaatag cattaaaata tgctcttata caaggcagtt ctactgtttc
    16801 cctccccaag gacaaaagag tttatgtttt tgccggcgac catggagtag tagaacaggg
    16861 tgtatctgca tacccaaaag aggttaccgc acagatggta ttcaacttcc ttgccggagg
    16921 agcagccata aatgttttca gtaaacataa caatacatca gtttatatag ttgatgctgg
    16981 agtagactac gatttctcat cagaacccag agtaataaac aaaaaagtag gaaggtcaac
    17041 caaagacttt tcttcaggac ccgctatgac ctcagaacag gctgagcttt gcataaaaca
    17101 cggccgtgaa gttgctacac aggccataaa tgatggcgct gaccttctag ctataggcga
    17161 tatgggaata ggcaatacca caactgcggc agcaatatgt gcgggactgg gaatacccgt
    17221 tgaagaagtg cttgatgtag gaacattgat agacaaccag atgctaacaa aaaaaggaga
    17281 agttcttgaa aaaaccatga aactgcattc acctttcaaa agccctatgg atgttttaat
    17341 gaaagccggt ggattttgta tcggcgaaat ggcaggattt atgcttgaat gcgctgaccg
    17401 aaaagttcct tttgttgttg acggcttccc cgtttcaagt gcagccctcc tggcttacag
    17461 aataaaccca aggatacgtg actacatgtt cataggccat cagtcagccg taaaaagtca
    17521 tctacccata ataaaagaac ttggactgga acctattctg aagcttgaaa tgagacttgg
    17581 cgaaggaaca ggagcagtac tctccttcaa cataatagag gcagccctga aaatgctcaa
    17641 tgaaatgagt agctttgaag atgccaatat atcaaagagt gatatagaat gattcttata
    17701 accggaggaa ttaaatcggg caagagcagc tacgccctgg aactggtaca aaaatatccc
    17761 agaaaagcct ttatagcaac tgcagaacca tttgacgagg agatgaaaca gcgtataaaa
    17821 aaacacaagc aggagcgtga cagttcttac gatacatatg aagaaccctt ggaagttgcc
    17881 tctctgctcc ctaagctgac agactacgat gtcatccttg ttgaatgtat aaccacatgg
    17941 ctttcaaatc tactttacta tgaaaaggat attgaaaaat acaccgaaag ttttatgaac
    18001 aatcttacag gcaaagaggt tatcataacc aacgaagtcg gactgggaat agttccgatc
    18061 gaccctctgt ccagaagata cactgatgcc attggaaaac ttaactcaaa acttgcccgt
    18121 aaagcagatg aagtttacct tatggtaagc tcaatcccgg tgaaaataaa atgataggct
    18181 caacttcatg ggtagtaccc ggaacttact atgaaaatgc cgtcatactc caaaaatatg
    18241 ttgattttat agaacttctg gttttcacat gggacgaaga aaccaaaaaa ctttttgata
    18301 aagaactcaa acatcttaaa aaacttaaca tcagatactc tgttcatctt ccgacagata
    18361 cagccgaaaa cgctgcaaag gttatagagt acttttcctt gaacgacttt gaattactga
    18421 actacgttct gcatcctatg cccggatggc aaaaactgga aaaactccct ggtgtttcag
    18481 ttgaaaacct tataggaaca cttgaatatt ctgaaaagat gacctttgac ataggacacc
    18541 atattctcgg aaaaaaattt gatgctgcat ataaaaccaa tatagttgaa taccatctga
    18601 tgggagtgga aaacagcaca gaccacatcc cgttaaatga ttacgccata gacacattga
    18661 aagagtttta tttacccgga agactttgct gctttgaagt gtttgaactt gaaggacttt
    18721 taagctcatt gcggaggtgg aaagagtatg aaaaaaattc tcaatgaaat acgcatgact
    18781 gtctcttttc tttcacgtat tccggcaggt ggaaccggag aaattaaaaa cattccaaga
    18841 tacttcacaa tagttggata tctttcgggt atgatataca tgcttcttac tcataccttg
    18901 ggtaaagggg tttttcagac cgttcttact atgtccaggt tttttttgct tcgacctttt
    18961 ccactttgac ggacttttag acactcttga tggtttccct ggtctgctca tcgctcacgc
    19021 ggactgaaaa a
//
LOCUS       DAOSHR010000015        19014 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_2344_length_19014_cov_5.735271, whole genome shotgun sequence.
ACCESSION   DAOSHR010000015 DAOSHR010000000
VERSION     DAOSHR010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 19014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19014
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2344_length_19014_cov_5.735271"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(164..1249)
                     /locus_tag="PLU28_01565"
     CDS             complement(164..1249)
                     /locus_tag="PLU28_01565"
                     /inference="COORDINATES: protein motif:HMM:NF024319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOG33784.1"
                     /translation="MGMGKFLLELTKMFSMYRWNTRPSLIKFTEADNAYHGLVLSLFS
                     ICSQESSTAMTFLKKRIVKEFPKIVLSDISLDTKNRIILKNSSVWDKVLQSTYCDLKL
                     IIDDETINSAIFIEDSLSQEDSKKSALISLLVALGEVQLNRKVFPQYYSQSYEELKEK
                     LQLMDYIGKDELIELSESLFHTTLRLNTIIRWNNNHRNVISSVASHSFFVTASTLFLT
                     IIYGCDENTAYKALAAAILHDLPEAFTGDVITPTKKKVEGLEEIISEIENDFIDEWKG
                     SNSLIKEKMDCLQDFMINPFASDYGRIVKNSDILAAMTECAVEIKTGNQNEIFRKAFF
                     SSKKELKKYSVTDISDILDEIEYLTFF"
     gene            complement(1262..2545)
                     /locus_tag="PLU28_01570"
     CDS             complement(1262..2545)
                     /locus_tag="PLU28_01570"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HOG33785.1"
                     /translation="MKIFDKLKLISPDFVGMLTPEGINGEVTNNFYTFRTKDTEMLIY
                     FNDFYMVSPLDIKSINYLLEKDTDNFSKLQDEIDYTKFVEAIKNLNKEYKLNKLLMSI
                     EKELAALLKCDGTSTLLYNASKNVLNFFVISGKASGKIETIDVPIEGSIAGYIFKNDR
                     SYIFNDIESTPFHFKQTDKKSGFHTYNIIGTTLRYHNIPVGVVEAVNKKSGFSDIDLE
                     ILETFAIIMSNKLLNSKLYEDMSRLSKDVILSFASAIDLRDKYTHMHSQNVTAYSVSI
                     AKEMGYDDNFTDTIEIASLIHDIGKIGIPDEILNKKDKLTEEEFNIIKSHTTLGAEIL
                     SDVDLINNQILNGPLEHHEKLDGSGYPYGKKDDQISDIGKILSVADIYDALTTERPYK
                     KAWNKEDAIAFLRKDQDIKHSKKVIDALELFLSKI"
     gene            complement(2585..4684)
                     /locus_tag="PLU28_01575"
     CDS             complement(2585..4684)
                     /locus_tag="PLU28_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG33786.1"
                     /translation="MIRKLLLITVFILSAIGCFGYIKATADSTQLQIGKETVITIETD
                     GERVFADTKIGGFDNPDVLFDGIAVKNGKVSLIFLAPYKENVAYVKFTNSKKESTEVH
                     ITISDTKLAGEAVALKVTNIKGPSVYKDASSDIWNPLKKDMLISEGYEILNPKGAYVK
                     LNGPQEINIELTENSQIIFETLQKSGDEIDFRYKVKKGLTYNTIDKKLLPGSKFIVSD
                     NQSVVAGVRGTRFAFESGEKSIIRVFEGIVSLSLDDKALFADMNKMVVFDVFNKIADL
                     KELDIKEDELIKELEPKEEEIEPEKQPDEKEEDKTEKSGSTSDNKPAEEAVTSDLTNL
                     NIGNVKKNGTDYFIYSFAPEFKFGFLKLGIGLNAYQKELNGTMYYGIPSATDSTDPLS
                     AVTLNRLGIDLGFLNLDYGRSPYYTYALGMLANRYFVPNALVFDTAFNLYNISVWAHI
                     PYELASFVPFSFNQSSPLFSAGIKLNAFDTIIDGTYIYESSVLTSKPFEHAAILSLYK
                     TLLGIKFGAEADLLFGGSLKEKLAYGGFLGSFFDIPPFAQFSLGLSYSGDYFEPQLLG
                     PLYERKRELQTIKLPEKPYGLGAVGNLSLNIFNFASIDIGYKNYFLGEGLDVLSGTLA
                     VSLKNIVGSMPDFTVGGAYLNKGLFTNGTFNIWDSDTFFEVYVFYPVIGTNGLKYKFY
                     YDPETAKFVNSVIFESK"
     gene            complement(4686..5324)
                     /locus_tag="PLU28_01580"
     CDS             complement(4686..5324)
                     /locus_tag="PLU28_01580"
                     /inference="COORDINATES: protein motif:HMM:NF023178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HOG33787.1"
                     /translation="MTVFFYFFLSHMLGDYVFQTSFIARNKNKNIYVLILHILIIYAC
                     MYIVILPKGLTNNCLLILLSVMHFFIDIIKFKYGKNDFFKGADYYLFDQLIHLLTLLI
                     VSHFFVGQEMYLSYDLVLKSSVGLFNAFTIGILSGYIFNDMTKYKKDYFGYLIRFSIP
                     FLPNLWFLLLSVPVYVYFILRFMDTQKSKQNHEKLFSLSLSYIITIIILEVA"
     gene            complement(5321..6622)
                     /locus_tag="PLU28_01585"
     CDS             complement(5321..6622)
                     /locus_tag="PLU28_01585"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HOG33788.1"
                     /translation="MINRIIDYIKELNKNGYTIIYEIDNINNYQNAFEIHFEKCVASL
                     YYSEKVKLKFFSVNKDKLQYILVLDKMIRDYLKIGDIALEDILFQVMKITTSISVNSD
                     IYETSSSILEHISKIFSFSSSSILFLNDDEKNLETRYINKLSTENNETGIPVKGTLAG
                     ESVLVNLPICVNDPSLHPGYITIEGFDPDSKNIMNFSCIPFTFENKSFGTLVFITTKR
                     YFDLIDYYSFFLISQIVSSVMEVTIKEEKIKSMLMSTLNALLKAQNVRVKNDYVHENK
                     VQELALKIAEKEKVSSEDKWILSMATYIYDIGEIGIPNSILSKKEVLTEEEKKIIRSH
                     VELGYQLVKNIPSLPDKLKTIIHQHHERWNGEGYPTGLKGDEIDFFAQIIGLCDSYIA
                     MTQERPYRSALPKEKALEIIRNYSGIFFNPLLVKTLCEVLE"
     gene            complement(6603..8561)
                     /locus_tag="PLU28_01590"
     CDS             complement(6603..8561)
                     /locus_tag="PLU28_01590"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOG33789.1"
                     /translation="MIKKVKILLSVLLILLFIISPYIKNVWIEIFELKTLDLRYKIRG
                     TDKIESDVVVVAIDEESIINMEAGENNDSWPWERRHFGQLVKKLFENGAKTIGFDISF
                     TSPDEKDERNDTYFASVLRRYPKVILGSYIIVDSSIFTKYPEEYSRYLLQNTRYLDHS
                     LKIMNIENVNNTSLFRVYKIVPAIKEFSELVPVTSYEIGALDVDGIVRNMPLFFNEVW
                     AEENGFGLTLIPHMDVMLYAQFIDAQDIIVDFKDSSLNIFDKKIPFDNYGRFQIYYYG
                     KGREVFKQISFYDIMNDNFDSKDINNKVVIVGYTDTAKGLYDLRVTPFSTEEAGVYIH
                     ANAVQNFIRNEVLERPGFIWRILYLVICMVISLLLLAFTNPYRKLIAFVVPLFVIIFS
                     YITFTSRIWLDMFFPLLSTVIIISTDTVFAFLSEYREKKKIKGFFYKYVPDTVAKEII
                     KSSNLNLGGKVYNAVILFSDIQGFTEKSEKMNAEGVISFLNEYLTAMADVIRNRYNGT
                     IDKFIGDAIMSIFGAPVTYGDEVSRALNCALDMKEELKKLNQKLNLPFELKNGIGIHF
                     GEVVIGNVGAPFRMDYTAIGDTVNTSSRVEHLTRDLNQEILVTETVYEMAGDTFIFEA
                     MGDFSVKGKSKPLKLYSLKGRKNDKQNN"
     gene            complement(8676..9893)
                     /locus_tag="PLU28_01595"
     CDS             complement(8676..9893)
                     /locus_tag="PLU28_01595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease family protein"
                     /protein_id="HOG33790.1"
                     /translation="MSDLSSGYKIGGWKKYVFSLQHFIAMFGATVLVPLLTGIDPLLA
                     LFTAGLGTLLFHTVTGFKVPVFLGSSFAFIAPIVIVRDKFGDLAYSTGAIAIAGLVYL
                     LFALLVKLLGYEKIKKLFPPVVTGSMIIIIGLSLSPVAIQWASSNWLISLCTLAAVIF
                     FSTFFKGFFSMIPVLMGVLIGYAVAVATGNIDFQAVAQADWIGLPKFVFPKFDISAIL
                     MTVPLTIATFMEHVGDITTNGAVVGKDFVKDPGLHRTLMGDGLATSLAGILGGPANTT
                     YSENTGVLALTKVYDPSVIRGAAILAIVLAFFSKFGSVLQTIPQSVIGGVSLVLFGMI
                     ASVGIRTLVNEKVDFANSKNLLIGAVMLTLGIGGASLTLGNVELKGVALSAIAGITLN
                     LILPDKDKKKINV"
     gene            10104..11252
                     /gene="dxr"
                     /locus_tag="PLU28_01600"
     CDS             10104..11252
                     /gene="dxr"
                     /locus_tag="PLU28_01600"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:TIGR00243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HOG33791.1"
                     /translation="MSKKVFIVGATGSIGDQTLDVLRTLNAQGFEYRVVGISLNSRWE
                     RAVPIIEEFSPRFCCVADNNVAVPQKYNSTVIRRGFESINSSIEELCPDITVVGSSGF
                     RSLSNTLCAIRNSERVCLANKESIVCGGNMIFNAAQRYSTQIIPVDSEHSAIFQLLQG
                     EKSAIQELIITASGGSLRDLPLENFTSITPEKVLSHPVWKMGARITVDSASMINKGLE
                     VMEAFYLFKTSSIKVLINRKSMVHSAVKFTDGVYKIHYGRADMRIPIAYSITYPQRSR
                     LDYGGPDLYSEPLLFENVDYQRYPSLKLAFDILGNQLLHIAYNASDEAAVEMFLNKRI
                     SFPDIYKLVCAVISSVNESKTELNDLEEISFYDELFRRKTYEIIGGDR"
     gene            11249..12769
                     /locus_tag="PLU28_01605"
     CDS             11249..12769
                     /locus_tag="PLU28_01605"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOG33792.1"
                     /translation="MMAIVWFLLIITVLVVVHEFGHYLAAVSLKTRVEEFAIGFGPAL
                     FKLKGKYTQFRFNVFPLGGYVRLAGEDNGNENYDPSDNGLFINKKPWQKLVIALAGPL
                     FSILLGYIVIGFSGMVYGFNEVVIEKVIPGSPADEAGLLSGDIIKKVNSGYAFDRSIL
                     SLNIAEGKKVDMQVVSSGKIKELSVFPSYTEQVDTIVLDYFEYEGNLEELSNKELLKI
                     NGSDKIGEVFSRLEAGDPINLEFEGETFLRGKLSSYSAEKERYTIGVYFAVFSNIINI
                     NQGKFQKGDKIIAIDDFEINGGTDFLESITKLSLSSGQFYLQTEANRTTKYFSPFLGN
                     DIFIKILREGNEIIINIEKSEFMQLLSSVNAVAQGYGKWRPKGAEVIGVSIQWANSLL
                     KAMLQSIGQLFTGKADTNDLMGPVGIANIIGQAAQAGIESILSLIALITLNLGIFNLL
                     PLPALDGGRIVFSLYEMISRRKVNPKVEAIVHFIGFALLMVLMVYVTFNDITRFMK"
     gene            12777..13820
                     /gene="ispG"
                     /locus_tag="PLU28_01610"
                     /gene_synonym="gcpE"
     CDS             12777..13820
                     /gene="ispG"
                     /locus_tag="PLU28_01610"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HOG33793.1"
                     /translation="MFSKKTVKVKNIFIGGEHEIVIQSMTNTDTLDISSTLNQINNLY
                     ELGAQLIRVSVRSLDDIESFRIICASSKVPLCADIHFDYRVAVESIKAGASKIRLNPG
                     NIGAEWKVREVVNTAREYGVPIRIGVNSGSIKKEFSYLERYKALAESCLEELRILESM
                     EFGDVVLSLKSSDAMETYNANLYVAGKVDYPMHLGVTEAGVWEDSIILSSAGIGSLLF
                     NKIGDTIRVSISGDPLQEVTAAHKMLTLLGYLKGPRVIACPTCARTQIDVAELAKKVK
                     IMTADIHQQITIAVMGCVVNGPGEAKDADIAVAGSGGAGVIFVKGKIVKKVERSNLEK
                     ELMDEIQKMICGR"
     gene            13824..14297
                     /locus_tag="PLU28_01615"
     CDS             13824..14297
                     /locus_tag="PLU28_01615"
                     /inference="COORDINATES: protein motif:HMM:NF015822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaR family protein"
                     /protein_id="HOG33794.1"
                     /translation="MFINPASDNKNPESEKIKNKKVNKKKSSSTGKSEESSDDFFDIL
                     LTKDYELTEKELKKLIDMIFGFGNSFVRSPTEWNLKRYKSAIKEYLQKIEKKLYKIKE
                     ELGMDVDYPRLHVIAEVIDEKIGQITQAVFKKEKDTLFYASRIGEVNGLLVDLYR"
     gene            14310..15323
                     /locus_tag="PLU28_01620"
     CDS             14310..15323
                     /locus_tag="PLU28_01620"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG33795.1"
                     /translation="MFEEWLEKIIINLNSYKGCSLCMVSFDGTLNKHVAVEISKRTEG
                     LSPYEVMTIKPDKGNIKIDSVREIKEFSLCRPVYSDKKVVIINDIETMTVEAINAFLK
                     ILEEPPDFMIIIAFTTRWNSLLSTVKSRFLKINIPFPMEMRKELAERFKENLNLIAPV
                     INSDYEIIRYLLESDKESIVETAKNYFDTTDIDFLIDSLSVGLSTVPERLGFIFSYFR
                     FLTVISAMNEKNFFNTVASVGRVKETVDPLEFLKYLSKNGISFLHDAFVCKLGTRWKY
                     MDNSGAAIFFGTQNLNFNSNIIYDTIRHFEVLSAATVSKFNFELEVFSHFFRIAECFF
                     LLK"
     gene            15337..16260
                     /gene="ricT"
                     /locus_tag="PLU28_01625"
     CDS             15337..16260
                     /gene="ricT"
                     /locus_tag="PLU28_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HOG33796.1"
                     /translation="MLEMTANVYGIEQMELEKIYYFSYIGDQEEIKKGDLVIINTELG
                     EDIGKVVFGPREMSFDQIGYEPTTIIRRLTSEDMVEYNKALCEACEVTEYTKEKVKEL
                     NLKMRCLKSKYTFDKSRLVIYFGADSRVDFRELVKELAKKYRVRIELRQVGIRDEVKI
                     KGSLGLCGQVSCCCRFLREFDSIKMELAKTQQMMINTAKISGRCGKLLCCLKYENQFY
                     EEVMQGVPYENTTIEYDGKPARVITVNVFLKEVTLQTIEENQPILIKVPFDFFKNNSF
                     ITFETEKESFRGKNKEQAFYEEDSEDITNGK"
     gene            16280..17005
                     /gene="rnc"
                     /locus_tag="PLU28_01630"
     CDS             16280..17005
                     /gene="rnc"
                     /locus_tag="PLU28_01630"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOG33797.1"
                     /translation="MFNEKKVIENAIKEFNLPQGINEQLIFNSLCHTSYVFEKSNKDR
                     HISSNERLEFLGDAVLELVLSEYLYLEYKLSEGDMSKIRATVASEVVLSEVAKEIGVE
                     KYIFLGSGEEKYGGRMKSSILADTTEALIAAIYLSLGYEYAKSFILKYIDFYVKEAIN
                     GVLFLDYKTKLQEITQEKYKVLPEYVLIDSNGPDHTRLYTIEVRINGLSCGTGNGQSK
                     KIAEQLAAKQACQKILTEELKSE"
     gene            16998..17930
                     /locus_tag="PLU28_01635"
     CDS             16998..17930
                     /locus_tag="PLU28_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG33798.1"
                     /translation="MNEPSYINLYKTGELKRRKELLYKKLRSCDLCPHRCMVDRTEKP
                     GYCKVRDMTRISEYVLYPGEEPPLTGKTGAGGIFFSNCNMNCIYCQNFKFSQEGFGKE
                     ITVEGLSEIFLEIQNNKKAVNLDLITATAYLPFIYDALIIAIEKGFRLPIVWNTSSYE
                     SVETLKLLDGVVDIYLADIRYTSNAIGKKYSGVKDYWTVCEQAIFEMYRQIGNDFILD
                     NENILKRGIILRILVLPSNIQQAIQALKFIKYDLSDRIAVSLMDQYVPVNHTKNFPEM
                     DRFLKKSEYEAVVDVMQELELINGWVQEHKLIDD"
     gene            17957..>19014
                     /locus_tag="PLU28_01640"
     CDS             17957..>19014
                     /locus_tag="PLU28_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOG33799.1"
                     /translation="MTKEQLVLEILNQLQEGILAVDEREDIIFFNNSACGILGVEKDA
                     VLYKNVVDTIPNTRLHVILRTGVSELDRLQNLGNKVIITSRFPIRNEENEIVASVAVF
                     RDVTTVQKLAEEVTNLREIEAMLTSVIDSTTDAISVADEEGRIVMVNRAYTKITGLTP
                     REVIGKPATIDIAEGESLHIKCAREKKPILNVKMQVGQNKKDVIASVTPIFVKNDFKG
                     SVGVIHDISEIQNLLNELEATKRLLKKEKARHTFNDIVAVSSAMQNVIKQSQKAAETK
                     VNILIQGEVGVGKEVLAQAIHNSSERSSKPYLKINLGLVMPDKQEEFLFGERSYFIKA
                     DGGTLFIENIQSATPFVQS"
BASE COUNT         6331 a         3048 c         3523 g         6112 t
ORIGIN      
        1 ctgatgatct caccattata catatctagg attggagata aataaagctt tctgcaacta
       61 gcataagtct agactcatct gaatactttc tttttcttga tataataaaa cccctttcgt
      121 cagattctgt ctaactaaag gggttcaatt caaataacct agatcaaaaa aatgtaagat
      181 attcaatttc atcaagtata tcggatatgt cagttactga atattttttc aattcttttt
      241 tagatgaaaa aaatgctttt ctgaagatct cgttctgatt tccggttttt atctctacgg
      301 cacactctgt cattgcagca agaatatcgg aattcttaac tattcttccg tagtcagacg
      361 caaatggatt tatcataaaa tcctgtagac agtccatttt ttcttttatt aatgagttag
      421 accctttcca ttcatctata aaatcatttt caatttcgga aattatttct tccaagcctt
      481 caaccttctt ttttgtgggt gtgataacat cgcctgtgaa tgcttcaggc aggtcgtgga
      541 gtattgctgc ggcaagggct ttgtaagcag tgttttcatc acaaccgtat ataatggtaa
      601 gaaaaagtgt tgaagctgta acgaaaaatg aatgcgaggc aactgaactt atgacatttc
      661 gatgattgtt gttccagcgt attatggtat ttaaacgaag agtggtatga aaaagtgatt
      721 cagaaagttc tataagttca tcttttccta tgtagtccat gagctgaagt ttttctttca
      781 gttcttcgta tgactgtgaa tagtactgag gaaaaacttt tctgtttaat tgaacttcgc
      841 ctaaagccac tagtaatgag ataagggcac tctttttaga atcttcctgt gaaagagaat
      901 cttctatgaa aatggcagag tttatagttt catcgtcaat tataagttta aggtcacaat
      961 atgtactctg taatacttta tcccatactg atgaattctt gagtataatc ctattcttag
     1021 tatctaggga tatatctgaa agcacaatct tgggaaattc ctttacaatt ctttttttca
     1081 gaaatgtcat tgccgttgag ctttcctgag agcagatgga aaaaagcgat agaacgagac
     1141 catgataagc attatcggct tcagtgaact ttattagact tggccttgta ttccagcggt
     1201 acatactgaa catcttagtg agttcaagta aaaactttcc cattcccatc ttatatcacc
     1261 ttcatatttt tgataaaaat aactctagag catcgattac tttttttgaa tgctttatat
     1321 cttgatcctt tcgaaggaaa gcaatggcat cttctttatt ccatgctttt ttatatggtc
     1381 tttcggtagt cagggcatcg tatatatcgg caactgaaag aatttttcca atatccgaaa
     1441 tctggtcatc tttttttcca taaggatagc cacttccatc aagtttttcg tgatgttcaa
     1501 gaggaccgtt aagtatctga ttgtttataa gatcaacatc cgaaagtatc tcggctccaa
     1561 gagtagtatg agattttatt atattaaact cctcttctgt cagcttatct tttttattaa
     1621 gtatttcatc aggtatccca atctttccta tgtcgtgtat gagagaagct atttctattg
     1681 tatcagtgaa gttatcgtca taccccatct cttttgcaat actaactgaa taagctgtaa
     1741 cgttttgtga atgcatgtgc gtgtatttgt cccttaagtc tattgcactt gcaaaagaca
     1801 gtatcacatc tttggaaaga cgggacatat cttcatagag ttttgagttt agaagtttgt
     1861 ttgacattat tatagcaaat gtttcaagta tttcaagatc aatatctgaa aacccacttt
     1921 ttttatttac tgcttcaact actccgaccg gaatattatg atagcgtaag gttgttccaa
     1981 tgatattata agtgtgaaac ccacttttct tgtcagtttg cttaaaatga aagggagtgg
     2041 attctatatc gttgaagatg taggagcggt cgtttttaaa tatatatccg gcaattgatc
     2101 cttcgattgg aacatcaatt gtttcaattt ttccgctggc ttttcctgag ataacaaaaa
     2161 aattaagaac attctttgaa gcgttataaa ggagtgtaga tgtcccatca cactttaaaa
     2221 gagcggcaag ttctttttca atagacatta atagtttatt gagcttatac tctttattca
     2281 ggtttttaat tgcttcgaca aacttggtat aatcaatttc atcttgtaat tttgaaaaat
     2341 tatcagtatc tttttccagg agatagttta ttgattttat atcaagagga gaaaccatat
     2401 aaaaatcatt gaagtagata agcatctctg tatcttttgt tctgaaagta taaaaattat
     2461 ttgttacttc gccgttaatt ccttctgggg taagcatgcc gacaaaatca ggagatatta
     2521 attttagttt atcaaagatt ttcatgattg cacctcaaca aaggcggcac atggccgcct
     2581 gtttttactt ggattcaaaa ataaccgaat tgacaaattt tgcagtttct ggatcatagt
     2641 agaacttgta cttaagaccg tttgttccta taacaggata aaacacatag acttcaaaga
     2701 atgtatctga atcccatata ttgaaagttc cgtttgtaaa taacccttta ttcagatatg
     2761 ctccgccaac agtaaagtca ggcatcgagc caacgatatt tttaaggctt acggcaagag
     2821 ttcctgaaag aacatcaaga ccttctccca aaaagtaatt cttgtagcct atatcaatag
     2881 atgcaaaatt gaaaatattc aaagaaaggt tcccaactgc tccaagtccg taaggttttt
     2941 ccggcaattt tatagtttga agttctcttt ttctttcata taaaggtcct aaaagttggg
     3001 gttcaaaata atctcctgaa tagcttaatc caagactgaa ctgtgcaaaa ggaggaatat
     3061 caaagaatga tccaagaaaa cctccatatg ctaatttttc tttcagtgaa ccaccgaaaa
     3121 gcagatctgc ttctgcacca aatttaattc ccaaaagggt tttatataaa gaaagtattg
     3181 cagcatgctc gaaaggcttt gatgtaagta ccgatgattc atatatgtat gttccgtcaa
     3241 ttatggtgtc aaaagcatta agctttattc ctgctgagaa taatggggat gactgattga
     3301 atgaaaatgg aacaaatgaa gctaactcat aaggaatatg tgcccaaaca cttatgttgt
     3361 agagattaaa agctgtatca aaaacaagag cattaggtac aaaataacgg tttgcaagca
     3421 ttccaagagc gtatgtatag tatggcgaac gtccgtaatc aagattcaga aagccaaggt
     3481 caatgcccag tctgttaagc gtaactgcgc ttaatggatc tgtgctgtct gtggctgaag
     3541 gtattccgta gtacatagta ccgttgagtt ctttctgata tgcatttaat cctataccaa
     3601 gttttaagaa tccaaattta aattcagggg caaaagaata gataaagtag tctgttccgt
     3661 tctttttaac gtttccgatg ttaagattgg ttaagtcact cgttacagct tcttctgctg
     3721 gtttgttatc tgatgttgat cctgattttt cggttttatc ttcttccttt tcatccggct
     3781 gtttttcagg ttctatctct tcttctttag gttcaagttc ttttataagc tcatcttctt
     3841 ttatatccag ttcttttaga tcggcaattt tgttgaaaac atcaaaaaca accatcttat
     3901 tcatatctgc gaacaaggct ttatcgtcaa gacttaacga aacaattcct tcgaatactc
     3961 tgattataga tttttcacca gattcaaaag caaaacgtgt acctcgtact ccggcaacta
     4021 ctgactgatt atcagaaact ataaattttg aaccaggaag taatttttta tctattgtat
     4081 tataagttag tccttttttt accttgtacc gaaaatctat ttcgtctcca gatttttgaa
     4141 gagtttcaaa aattatttgt gagttttctg taagctctat gtttatttcc tgaggaccgt
     4201 taagttttac atacgctcct ttgggattta atatttcgta gccttcagaa ataagcatat
     4261 ctttttttaa cggattccat atatctgatg aggcatcttt ataaacagag ggtcctttaa
     4321 tgttagttac tttaagtgcc actgcctctc cggcaagttt ggtatctgat atggtaatat
     4381 gtacttccgt gctttctttt ttagaattgg taaattttac ataagcaacg ttttctttat
     4441 acggagcaag gaatatgaga gaaaccttac cgttttttac ggctataccg tcaaaaagaa
     4501 cgtctgggtt gtcaaaccct cctatctttg tgtctgcaaa aactctttcg ccatcagttt
     4561 ctatagttat tactgtttcc tttcctattt gaagctgtgt tgaatcagca gttgctttta
     4621 tatatccaaa acaacctatg gcagagagaa taaaaaccgt aattagcagt aacttcctga
     4681 tcattttaag ccacctccag aataataata gtaattatat aacttaatga aagcgagaaa
     4741 agtttctcat ggttctgttt actcttttgc gtatccatga atcttaaaat aaagtataca
     4801 tagaccggaa cagataaaag taagaaccat agattaggta gaaatggaat tgaaaatctt
     4861 attaaatacc caaaataatc ttttttatac tttgtcatat cgttaaaaat atagcctgac
     4921 agtattccta tagtaaaagc attgaaaagt cctacagatg attttaagac caaatcgtag
     4981 ctcagataca tttcctgccc gacaaaaaaa tgagaaacaa ttaataaagt taacaaatga
     5041 atcagttggt caaaaagata gtaatcagca cccttgaaaa aatcgttttt cccatatttg
     5101 aacttgatga tatcaataaa aaaatgcatc acagataata agataagcaa gcaattattt
     5161 gttaaaccct ttggaagtat tacaatgtac atgcaagcat atataatcag aatatgaaga
     5221 ataagcacat agatattttt attcttgttt ctggctataa aggaagtttg aaaaacgtaa
     5281 tcaccaagca tgtgtgaaag gaaaaaatag aagaatactg tcattctagc acctcgcaca
     5341 aagtctttac aagtaaagga ttaaaaaata tacccgaata atttctaatg atctctagag
     5401 ctttttcctt tggtaaagcc gaacggtaag gtctttcctg agtcatagca atatatgagt
     5461 cacacaatcc tattatctga gcaaaaaaat ctatctcatc tcccttaagc cctgttggat
     5521 atccttcgcc gttccatctt tcatgatgct gatgaataat cgtcttaagt ttatctggaa
     5581 gagacggtat attttttaca agctgatatc cgagttcaac gtgtgagcgt attattttct
     5641 tttcctcttc ggtgagtacc tctttttttg aaagaattga gttaggtatc cctatctctc
     5701 caatatcgta tatatatgta gccatactaa gtatccattt atcttcggaa gatacctttt
     5761 ctttttcggc tattttcaat gctagctcct gaactttgtt ctcgtgaaca taatcattct
     5821 tgacccgtac attctgagct tttaaaagag cattcaaagt actcataagc attgatttta
     5881 ttttttcttc tttaatagtc acctccataa cagaggacac aatttgagag attagaaaaa
     5941 aactatagta atcgataaga tcaaaatagc gctttgtggt tataaaaacc aatgtaccaa
     6001 atgatttatt ttcaaaggta aaaggtatac atgaaaaatt cattatgttt ttggaatctg
     6061 ggtcaaaacc ttcaatagta atatatccag gatgtaaaga aggatcatta acgcatatcg
     6121 gaaggtttac caaaacgctt tctcctgcaa gggtaccttt taccgggatt ccggtctcgt
     6181 tattctcggt acttagctta tttatatatc tagtttcaag atttttctca tcatcattca
     6241 gaaaaagtat tgaagaagaa gaaaaactaa aaattttcga tatatgttcc aatattgatg
     6301 aagaagtttc atatatgtca gagttaacag aaatagaggt ggtaattttc attacctgaa
     6361 agagtatatc ctcaagcgca atatctccta ttttcagata gtcgcgtatc attttgtcca
     6421 gaaccaagat atactgaagt ttatctttgt ttaccgaaaa aaatttcaat ttcacttttt
     6481 cagaataata taaagatgca acacactttt caaagtgtat ttcaaaagca ttttgataat
     6541 tatttatatt atctatttca tatattatgg tataaccgtt tttattcagt tctttgatat
     6601 aatcaattat tctgtttatc attttttctc cctttaagtg agtaaagctt caaaggcttg
     6661 cttttgcctt ttacagaaaa atctcccata gcttcaaaaa tgaaggtatc tccggccatt
     6721 tcataaacag tttcagttac aagtatttct tggttaagat cgcgtgtcaa gtgttccact
     6781 ctggaagatg tgttaacagt atcgcctatt gctgtataat ccattctgaa aggagctcct
     6841 acattgccaa ttactacctc tccaaaatgt attcctatac cgttttttaa ttcgaatggg
     6901 aggttgagct tttgatttag tttcttaagc tcttccttca tatcaagcgc acagttaagt
     6961 gcccttgata cttcgtcacc atacgttaca ggagcgccaa aaatactcat aatagcatca
     7021 cctataaatt tatcgatggt tccgttatat ctgttcctta ttacatcagc catagcagta
     7081 agatactcat taagaaaact tattacacct tcagcattca ttttttcaga tttctctgta
     7141 aatccctgga tatcagaaaa aagaatcaca gcgttatata cttttccacc caaattaaga
     7201 ttgctgcttt ttattatttc ttttgcaaca gtatccggaa catatttgta gaaaaaccct
     7261 tttatttttt tcttttcacg atactccgaa aggaaagcaa aaacagtgtc tgtagatatt
     7321 ataattacag ttgacaacaa tggaaaaaac atatccagcc agattcttga ggtaaaggta
     7381 atataggaaa aaattataac aaagagcgga acaacgaatg caataagttt tctataagga
     7441 ttagtaaatg ccagcaataa aagcgagata accatacata taacaagata gagaatacgc
     7501 caaataaacc caggtctctc aagaacctca ttacgtataa aattttgtac agcattagcg
     7561 tgtatatata cacccgcttc ttcggtcgaa aaaggagtga ctctcagatc ataaagacct
     7621 tttgcagtat ccgtataccc aacaataaca actttgttgt ttatatcctt ggaatcgaag
     7681 ttatcgttca ttatatcgta gaaagatatc tgtttaaaga cctcgcggcc tttgccataa
     7741 taataaatct ggaatctgcc gtagttatca aaagggattt ttttgtcaaa aatattcaat
     7801 gaactatctt taaaatcaac aattatatcc tgtgcatcaa taaactgggc atagagcata
     7861 acatccatat gaggtattaa agttaatccg aatccgtttt cttctgccca tacttcatta
     7921 aaaaacaatg gcatattacg tacaatacca tctacatcaa gtgcccctat ttcgtaggaa
     7981 gtaacaggaa caagttccga aaactctttg atagcaggaa caatcttgta tactctgaaa
     8041 agagatgtgt tatttacatt ttcaatattc attattttta acgaatgatc aagataacgc
     8101 gtattttgta aaagatatct actgtactct tcagggtatt ttgtaaatat agagctatca
     8161 actattatat agctgccgag tattactttt ggatagcggc gtaaaacgga agcaaagtat
     8221 gtatcgtttc tctcatcttt ttcatcaggt gatgtaaaag aaatatcaaa tcctattgtt
     8281 tttgcaccat tttcaaaaag tttttttacc agctgtccaa agtgtcttcg ttcccaaggc
     8341 caactatcat tattttctcc tgcttccata tttattatgg attcttcgtc aattgcaaca
     8401 accacaacat cactttctat cttgtctgtg cctcttattt tataacgtaa atccagtgtt
     8461 tttaactcaa aaatctctat ccatacattt tttatgtacg gagagattat aaaaagcagt
     8521 ataagaagga cgctaagcag tattttgact tttttaatca tatctatcac ttctcattca
     8581 gagagtattt aacagcaatt taattatact aaaaaaaaaa ataaattaca aattaaatcg
     8641 gtaacaaaaa atggtgcata gcaccatttt tttaactaaa cattgatttt ctttttatct
     8701 ttatccggaa gtattagatt aagtgtaatt ccagcaattg ctgaaagtgc aacccctttt
     8761 aattctacgt ttcctaatgt aagtgaggct ccacctatgc ccaaagtcaa cattacggca
     8821 cctataagaa gatttttgga gtttgcaaaa tcgacttttt cattcactaa ggttcgtata
     8881 cctaccgagg caatcatccc aaaaagtact aaacttactc ctcctattac tgactgagga
     8941 atagtttgaa gaacagaacc gaacttagag aaaaaagcca gaactattgc aagtattgct
     9001 gcgcctctta taacggaagg atcgtaaact tttgtaagtg caagtacacc agtgttttca
     9061 gaataagttg tattggccgg tcccccaagt atacccgcaa gcgaggttgc aagaccatct
     9121 cccataagtg ttctgtgaag tccgggatct ttaacaaaat cttttccgac aactgctcca
     9181 tttgtagtaa tatcgccaac atgttccata aatgtggcta tagtaagagg cacagtcata
     9241 agtatggctg aaatatcaaa ctttggaaat acaaatttgg gaagtccaat ccaatcagct
     9301 tgtgcaactg cttgaaaatc aatatttcct gttgcaacag ctacagcgta acctataagt
     9361 actcccatga gtacaggaat catactgaaa aatcctttga agaaagtgga aaagaatatt
     9421 acggcagcca aagtgcagag agatatcaac caattagaag aggcccactg aatagcgacc
     9481 ggagaaagcg aaagaccaat aatgattatc attgaaccag ttactacagg tgggaaaagt
     9541 tttttgattt tttcatatcc cagcagctta actaaaagag caaagagaag ataaacaagt
     9601 cctgcgattg ctatggctcc tgttgaatat gctagatctc caaacttatc tcttactatt
     9661 acaataggag ctataaaagc aaaactggag cctaaaaaaa caggaacttt aaaaccagtt
     9721 accgtgtgga aaagcaatgt acccaaacca gcagtaaaaa gagctaaaag cggatcaatt
     9781 cccgtaagta gcggaaccaa aacagttgca ccaaacattg caataaaatg ttgaagtgaa
     9841 aatacgtact ttttccaacc acctatcttg taaccagagc ttaaatctga catatattcc
     9901 tcctttttaa cctcaccggg ctaaattaaa gtgcctaaac cgtacaaagg ttacatcaac
     9961 ggtattaact ttaagtgttt caaatatgta ttttatgtta atctaaagaa tgtataataa
    10021 gttaaatatt ggagcacata aattttcagc tatatattgt ataattaatt aagaaaaaat
    10081 aaagtaaaga ttgaggtgct aagttgtcaa aaaaggtatt tatcgtcgga gccacaggga
    10141 gtataggaga ccagacactc gatgtattaa ggactttaaa tgctcaaggc tttgaataca
    10201 gagtagttgg tatctctctt aattccagat gggaaagagc tgttccaata atagaagagt
    10261 tctctccaag attctgttgt gttgcggata ataatgttgc tgttccacag aagtataata
    10321 gcactgttat aaggagaggt tttgaaagca tcaatagcag catagaagag ctttgtcctg
    10381 atataacagt agtgggatcc tcaggtttta gaagtctttc aaacacttta tgcgcaataa
    10441 gaaattctga aagagtgtgt cttgcaaaca aagaatcgat agtatgcggt gggaacatga
    10501 tttttaatgc agctcaaagg tactctacac agataattcc tgtggatagt gagcacagtg
    10561 caatttttca gcttttgcag ggtgaaaaga gtgctattca agagttaatt ataacagcat
    10621 cgggtggttc tttgagggat cttcccttag aaaatttcac cagtataact cctgaaaaag
    10681 ttctcagcca tcctgtttgg aaaatgggag ccagaatcac tgttgattct gcaagtatga
    10741 taaataaagg attagaagta atggaagcat tttatctttt taaaacttct tctattaagg
    10801 ttcttattaa cagaaaaagc atggtgcatt ctgctgtaaa atttactgac ggagtataca
    10861 agatccacta tggccgagct gatatgagaa taccgattgc atattcaatc acctatccgc
    10921 aacgttcaag attggattat ggaggacctg atctgtattc agaaccgtta ctttttgaaa
    10981 atgttgacta tcagaggtac ccttcgttga agcttgcttt tgatattctt gggaatcagt
    11041 tgcttcatat agcctacaat gcaagtgatg aagctgcagt agagatgttt ttaaataagc
    11101 ggataagctt tccagatatt tataaactgg tttgtgctgt gataagctcg gttaatgagt
    11161 ctaaaacaga gctcaatgat cttgaagaaa taagttttta tgatgaactt ttcagaagaa
    11221 aaacatatga aattattggt ggtgatagat gatggctata gtatggtttt tgcttataat
    11281 aactgtttta gttgttgttc acgaatttgg acattatctg gcggctgttt cactgaaaac
    11341 aagagttgaa gagtttgcca ttggttttgg tcctgcgctt tttaagctta aaggcaagta
    11401 tactcagttc agattcaatg tttttccttt aggtggctat gtgagacttg caggagaaga
    11461 caacggaaat gaaaactatg atccttctga taatggactt tttataaaca aaaaaccatg
    11521 gcaaaagctt gttattgcac ttgctggacc tttattttca attcttctgg gctatattgt
    11581 cataggattt tccgggatgg tgtatggatt caatgaggta gtaattgaaa aagtaatacc
    11641 tggttctcct gccgatgaag caggcttact ttctggagat atcatcaaaa aagttaactc
    11701 tgggtatgct tttgaccgtt ctattttgag tcttaatatt gcagaaggta aaaaagttga
    11761 tatgcaggtt gtgagttccg gcaagataaa ggagctttct gtttttcctt cttatacgga
    11821 acaagtggat actatagtac ttgattactt tgaatacgaa ggaaatcttg aagaactttc
    11881 caataaagaa cttttaaaaa tcaacggatc ggataaaata ggtgaggttt tttcaagact
    11941 tgaagcaggt gaccctatca atcttgagtt tgaaggagaa acttttttaa gaggaaagct
    12001 tagttcatat tctgctgaaa aagaacgtta tactataggt gtatatttcg cagtattctc
    12061 aaatatcata aatattaatc agggaaaatt tcaaaaaggc gataagataa tagcaataga
    12121 tgattttgaa ataaatggag gaaccgactt tcttgaaagc ataactaagt tgtctctttc
    12181 ttcaggacag ttttatcttc agacagaggc aaaccgtaca acaaaatact tttctccttt
    12241 tttggggaac gatatcttta taaaaattct aagagaaggt aatgagatta ttattaatat
    12301 tgaaaaaagt gagtttatgc aactcctttc ttctgttaat gcagtagccc aggggtatgg
    12361 aaaatggaga ccaaagggag ccgaagttat aggagtttcg atacaatggg caaacagtct
    12421 gcttaaagca atgcttcagt ccatagggca gctttttaca ggtaaagccg acacaaatga
    12481 tctcatgggg ccagtgggaa tagccaatat aattggtcag gctgctcagg ctggaataga
    12541 aagcatatta agccttattg cccttataac gttgaatcta ggaatattta atcttcttcc
    12601 tttacctgca ttagatggag gacgaatagt attttctctt tatgagatga taagccgcag
    12661 aaaggtaaat cctaaagtag aggcaatagt tcattttata ggatttgctt tacttatggt
    12721 tctgatggtg tacgttactt ttaacgatat tacaagattt atgaagtagg tggagtatgt
    12781 tttcaaaaaa aactgtaaaa gtcaaaaata tttttatagg cggcgagcat gaaattgtaa
    12841 ttcaatctat gacaaatact gatactttgg atataagctc tactcttaat caaatcaata
    12901 atctgtatga acttggagct cagttaataa gagtatctgt aagatctctt gatgatatag
    12961 agtcttttag aataatatgt gcaagttcaa aggttccgtt gtgtgcagat atacactttg
    13021 attacagagt ggctgttgaa tcaattaaag caggagcttc taaaataaga cttaaccctg
    13081 ggaatatagg cgcagaatgg aaagttcgag aagtggtaaa tactgcaaga gagtacggag
    13141 tcccaataag gataggtgta aattctggct cgataaaaaa agaattttca tatcttgaaa
    13201 ggtataaagc acttgccgaa agctgtcttg aagagttaag aatacttgag tccatggaat
    13261 ttggtgacgt tgttctttcg ttgaaatcat ctgatgctat ggagacttat aatgcaaatc
    13321 tttatgttgc cggaaaggtt gattatccga tgcaccttgg agttactgag gcaggagtat
    13381 gggaggactc tattattctc tcatctgccg gaataggttc tcttttattt aataaaatag
    13441 gagatacaat cagagtatct atttcaggag atcctcttca ggaggtaact gcagcccata
    13501 aaatgctgac tcttttggga tatcttaaag gcccaagggt tattgcgtgt cccacatgtg
    13561 ccagaactca gatagatgtt gctgagctgg caaaaaaggt aaagataatg acagcagata
    13621 ttcatcagca aatcactata gcagttatgg gatgcgtagt caatggtccc ggagaagcaa
    13681 aagatgctga tatagcagtt gcaggaagcg gcggagccgg agtgatattt gtaaaaggaa
    13741 agatagtaaa aaaagttgaa agatccaatt tggagaagga attaatggat gaaatacaaa
    13801 aaatgatttg tggaaggtga tatatgttta ttaatcctgc ttctgataat aaaaatccgg
    13861 aaagtgaaaa gataaagaat aaaaaagtta ataagaaaaa atcttcatca acaggaaaaa
    13921 gtgaagaaag ttcagatgat ttttttgata tactacttac taaggactat gagctcacag
    13981 agaaggaact taagaaattg atagatatga tttttggatt tgggaattct tttgttcgtt
    14041 caccaacaga atggaatttg aaaagatata aaagtgctat aaaagagtat ttacaaaaga
    14101 ttgaaaaaaa actttataaa attaaagaag aactgggaat ggatgtggat tatcccaggc
    14161 ttcatgttat agctgaagta attgatgaga agataggtca gataacccag gcagtgttca
    14221 aaaaagagaa agatactctt ttttatgctt caagaatagg tgaagtaaat ggtcttttgg
    14281 ttgatctgta ccgttgagcg gagataaaaa tgtttgaaga atggttggaa aaaatcatta
    14341 ttaacctgaa ctcatataaa ggatgttcct tgtgtatggt ttcttttgac ggtactctta
    14401 acaagcatgt tgcagtagaa atttctaaaa gaacagaggg actaagtcct tatgaagtaa
    14461 tgacaattaa gcctgataaa ggaaatataa aaattgactc tgtacgtgaa ataaaggagt
    14521 ttagtctttg tcgtccggtt tattctgata aaaaagttgt tataataaat gatatagaga
    14581 ctatgacagt cgaagcaatc aatgcttttc tgaagattct tgaagagcct cccgatttta
    14641 tgattataat agcttttaca accagatgga actctcttct ttccacagta aaaagcagat
    14701 ttttaaagat aaatattcct tttcccatgg aaatgaggaa ggagttagca gaaagattta
    14761 aggaaaatct taatttgatt gctccggtta taaactctga ttatgaaata attagatatc
    14821 ttctggaatc tgataaagaa agtatagttg aaactgcaaa aaattatttt gatactaccg
    14881 atatagactt tcttatagat agtctttctg taggcttaag tacggtgccg gagcgattag
    14941 gattcatttt ttcatacttc cggtttttga cagttatttc tgcaatgaat gaaaagaatt
    15001 tttttaatac tgttgcaagt gttggtcgtg taaaagagac tgtagatccg cttgaatttc
    15061 ttaagtacct atccaaaaac ggtatttctt ttcttcatga tgcatttgtc tgtaagcttg
    15121 gaaccagatg gaagtatatg gataactccg gtgcagctat ttttttcgga acacagaatc
    15181 ttaattttaa cagcaatatt atttacgaca caataaggca ttttgaggtg ttaagcgctg
    15241 caacagtatc taaatttaat tttgaacttg aagtattttc acattttttc aggatagctg
    15301 aatgtttttt tcttttgaaa taaggaggat aatagaatgc ttgaaatgac agcaaatgtt
    15361 tatggtatag aacagatgga gttagagaaa atatactatt tttcttatat tggagatcaa
    15421 gaagaaataa aaaaaggtga tcttgttata ataaatacag aacttggaga agatatcgga
    15481 aaagttgttt ttggtcctag agagatgagc tttgatcaaa ttggatacga acctacaact
    15541 ataatacgca gacttacgtc agaagatatg gtagagtata acaaagcact atgtgaagca
    15601 tgtgaagtga cggaatatac caaagaaaaa gtaaaagagc ttaatctgaa gatgagatgt
    15661 cttaaatcga agtatacttt cgataagtca aggctagtaa tctattttgg agctgactcc
    15721 agagttgatt ttcgtgagct ggtaaaagaa cttgcaaaaa agtacagagt caggattgaa
    15781 ctgagacaag ttggtatccg tgacgaagtc aaaataaaag gctctctagg actgtgtgga
    15841 caggtttcat gctgctgtag atttttaagg gagtttgatt caataaaaat ggaattagca
    15901 aaaactcaac aaatgatgat aaatacagct aaaatttctg gaagatgcgg aaagctcctt
    15961 tgttgtctca aatatgaaaa tcagttttac gaagaagtaa tgcagggtgt tccttatgag
    16021 aacacaacca tagagtatga cggaaaaccg gctagggtaa taactgttaa tgtttttttg
    16081 aaagaagtaa ctttacagac cattgaggag aatcagccca ttcttattaa agtaccattt
    16141 gactttttta aaaataatag ttttattact tttgaaacag agaaagagtc attcagaggg
    16201 aagaacaagg aacaagcttt ctatgaagaa gactcagaag atataacgaa cggaaagtag
    16261 tcagtacaaa ggagaagaca tgtttaatga aaaaaaagtt atagaaaacg caattaaaga
    16321 gtttaatctt cctcagggga ttaatgaaca gcttatattt aactccttat gccatacatc
    16381 ttatgttttt gaaaaaagta ataaagatag gcatatttct tctaatgaaa gactagaatt
    16441 tctgggagat gctgttcttg agctggttct ttcggagtat ctttatcttg agtataaact
    16501 ttcagaagga gatatgtcaa aaatcagggc aacagttgcg agtgaggttg tgctgtctga
    16561 agtagccaaa gaaataggtg tggaaaagta tatcttttta ggttctggtg aggaaaagta
    16621 cggtggcagg atgaaatctt ccatacttgc tgatacaact gaagcattaa ttgctgcaat
    16681 ttatttgtca ttgggttatg agtatgcaaa atcatttatc ttaaagtata tagattttta
    16741 tgtaaaagag gctataaatg gtgtactgtt tttagattat aaaaccaagc ttcaggagat
    16801 tacccaagaa aagtataagg tgcttcccga atatgtgctg attgattcta acggtcccga
    16861 ccatacaaga ctttacacaa tagaagtaag aataaacggt ttaagctgtg gtacaggaaa
    16921 tggccagtca aaaaaaattg cagagcagct tgcagcaaaa caagcatgcc aaaaaattct
    16981 tacagaggag ttaaaaagtg aatgagcctt cttatataaa tctttataaa acaggtgagc
    17041 tcaaaagaag aaaagagctc ctttacaaga aattgaggag ctgtgatctt tgtccacaca
    17101 gatgtatggt tgacagaacc gaaaaaccag gatactgtaa agtaagagat atgactagaa
    17161 tatcagagta tgttctttat cccggggaag aacctcctct taccggtaaa accggcgcag
    17221 gcggtatttt ttttagcaac tgtaatatga actgtattta ctgtcaaaac tttaagttca
    17281 gtcaagaagg atttggcaaa gagattacag tagaagggct ttcagaaatt tttcttgaga
    17341 tacaaaataa taaaaaagca gtaaatctcg atcttataac ggcgaccgct tatcttccgt
    17401 ttatatatga tgctcttata atagcaatag aaaaagggtt tagacttcct attgtatgga
    17461 atacttcatc ttacgaaagt gttgaaacgc tcaaactgct tgatggggtg gtggatatat
    17521 atcttgcaga tataaggtat acaagcaacg ctatcgggaa aaagtattca ggagtaaaag
    17581 attactggac tgtatgtgaa caggctattt ttgaaatgta caggcagata ggcaatgatt
    17641 ttattttaga taatgaaaat attcttaaaa gaggtataat attaagaata ctggtgctgc
    17701 cgtctaacat ccagcaggct atccaggctc ttaagtttat aaagtatgac ctttcagata
    17761 gaattgctgt tagtcttatg gatcaatatg ttcctgtaaa ccatacaaag aattttccag
    17821 aaatggacag atttctcaaa aaaagtgaat atgaagctgt agtagatgtg atgcaggagc
    17881 ttgaactcat taatggttgg gtacaggaac ataagctaat agacgattaa ttattaatat
    17941 attggggtga actattttga ctaaagaaca acttgtttta gagatactta atcagcttca
    18001 ggagggtata ctggcagttg atgagagaga ggatataata tttttcaaca attcagcctg
    18061 cggtattctt ggagtagaga aagatgctgt cttatataaa aatgttgtgg atacaattcc
    18121 taatacccgg ctgcatgtaa ttttaaggac aggcgtatca gagcttgacc gtcttcaaaa
    18181 tcttggaaat aaagtcatta ttacttctag atttcctata agaaatgaag aaaatgagat
    18241 agttgcttcg gttgctgttt tcagagatgt tactacagta cagaagctgg ctgaggaagt
    18301 aactaatctt cgtgagatag aagcgatgct tacatcggtt attgattcta caaccgatgc
    18361 tatatctgtg gctgatgaag aggggcgtat tgtcatggtt aacagagcgt ataccaagat
    18421 aaccggactt actcctcgtg aagtaatagg taaacctgct acaatagata ttgctgaggg
    18481 agagagtctt catataaaat gtgcacgcga aaaaaaaccg attttgaatg taaaaatgca
    18541 ggttggtcaa aataaaaaag atgtgattgc ttctgttaca ccgatatttg tcaaaaatga
    18601 ttttaaggga agtgttggtg taattcacga catatcggaa attcaaaatc tgcttaatga
    18661 gttggaggct actaaaagac ttcttaaaaa agaaaaagct agacatacgt tcaacgatat
    18721 tgtcgcggtt tcttctgcta tgcaaaatgt tatcaagcaa tctcaaaaag ctgcagaaac
    18781 aaaagtaaat attttaatac agggtgaggt cggagtagga aaagaagttc ttgcacaggc
    18841 cattcacaat tcaagcgaac gttcttcaaa accatattta aagataaatc ttggtcttgt
    18901 tatgcctgat aaacaagaag aatttctttt cggagaaaga agttatttta taaaggcaga
    18961 tggaggaact ttgtttattg agaatataca gtcagctact ccgtttgttc agtc
//
LOCUS       DAOSHR010000016        18985 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_2347_length_18985_cov_5.989963, whole genome shotgun sequence.
ACCESSION   DAOSHR010000016 DAOSHR010000000
VERSION     DAOSHR010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 18985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18985
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2347_length_18985_cov_5.989963"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            60..947
                     /locus_tag="PLU28_01645"
     CDS             60..947
                     /locus_tag="PLU28_01645"
                     /inference="COORDINATES: protein motif:HMM:NF003784.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose bisphosphate aldolase"
                     /protein_id="HOG33800.1"
                     /translation="MMQKQFEKMRSGKGFIAALDQSGGSTPKALALYGITKDSYTTDE
                     EMFDLVHQMRTRIITSPSFSSEHILGAILFEQTMDRKIEGVFTADYLWEKKSVIPFLK
                     VDKGLDQENEGVQLMKPIKELDLLLKRAVSRNIFGTKMRSLILKADINGIKKIVSQQF
                     DIAKKIIDAGLIPIIEPEVDIKSIDKKESEEILKSEILKHLQQLGKESHVMLKLSIPT
                     VDDFYSEIIKHPRVLRVVALSGGYTREEANEKLARNHGLIASFSRALTEGLNVKQSDK
                     EFDDYLKASVESIYKASIV"
     gene            1281..2546
                     /locus_tag="PLU28_01650"
     CDS             1281..2546
                     /locus_tag="PLU28_01650"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOG33801.1"
                     /translation="MKNFSKLFLVGFILIFSLVVFGTREVVFWHGLSRDPDKSSIDAI
                     INSFEKENPDIKVKMVLVPAAETDSSKLLTAVYAGTGPDLVYIDRFTVSQRAANNVLE
                     PMEDLLQKNGIDINAMKKEFYDFAIEECIFSNKMWALPFDTDVRVLLYNEKLLNSAGL
                     KEPPKDIYSMIKAAEKLTKEGKRGRYDLVGFIPWFAQGWPYTWIYAFEGNIFDNATEK
                     FIFATDPGIIEAYAWQKEWADRFGYETLNAFSSLSIGDLNPFTAGKLAMIVDGNWTIS
                     GIKQFAPKDFKYKVGGIPTKSGKPVTWAGGWSIAVPKGAKNPSDAAKLAYYFATKGQL
                     QYSVDTLHIPTYRNAVDEFLKADPSQKSFIELLENARTRPPLPVGALLWDKLVEARDY
                     VLTGKKSVEKALLDAQNEVQTEYNKVMKK"
     gene            2558..5236
                     /locus_tag="PLU28_01655"
     CDS             2558..5236
                     /locus_tag="PLU28_01655"
                     /inference="COORDINATES: protein motif:HMM:NF015600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HOG33802.1"
                     /translation="MKRKFLVICIFVLLSIVYARLPESSYWYPNDIIDWEYEADADAI
                     YNRASVPLANRMVAQALVPSADERIKVMALSIMNSSTSGMPSQGLIGSMKTYPFTFWQ
                     YIDYLVAWAGSAGEGVIVPPSADVTDAGHKNGVPVLGTIFFPPNVYGGRRNWVDQLLQ
                     KEDGHFIVADKLIEITKYYGFDGWFINQETTGCNETHAKLMKEFIQYVKSSAPWMTVV
                     WYDSMTSNGDIEWQGEVTDKNAFFMKDLSTNKKVSDEMFVDFRWQSTKRPDTVVNSIK
                     NLQKYGIDSSSIFVGFDLQASGYLTAFNWPKLIADNEKLMLSLGLYCPSWSYYDSKTP
                     QEFWNKEESLWIGEHKVLEYNAKKEFSQIDWGDYKQYRWRGIASFVNEKSPLTGTSFS
                     TSFCAGHGKSFYINGERKSQKEWYNRSLTDVFPTYRWKVSGKSVPEITVDYDDAFYGG
                     TSLKFQGNIGNNESSEVNLYAANLEIKADSCFKAAIKAKDNVVDVFYILKLSNGKEIS
                     IKADHPEGMWKETSTSLSEYMGKTITEISIRVVGKETAQYSLNIGKIALVPQSKKIIS
                     SVKGVQIDSIEFREGLYAQLLLHWQESKDAKFYEIYRVLPDQTREFVWASYNNYTYIS
                     EIKRFGKEDSSALEVVAVDGGMNRSLREFVVFNWPNYPVPKADFEISNTIAMPGEKIR
                     LNSLSSEVTEQLVWILPGATPEISYEVSPEVVYEKEGIFPVTLKAINSEGESVKTVDP
                     LIVITKKAANVKNMALNKNTYASSNVPSEKPSMAVDGTVENNSKWCAVGDLPHWLVID
                     FEKEVIINRVIVKHAQSGNESPDMNTQIYKIQVSNDAQQWTDVVSVDSNKKGVTEDSF
                     APAKARYLRLFIEKPTQGGDKAARIYEVEVYGLEAY"
     gene            5302..6183
                     /locus_tag="PLU28_01660"
     CDS             5302..6183
                     /locus_tag="PLU28_01660"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOG33803.1"
                     /translation="MRKNNSLNFFKGIFFISPWLVGFLIFTLLPFIASFYYTFTRFSI
                     TTKVQWTGTENYVNMFSDPLFFKSLMNTVWMTVGLVPIGLLVALGTAILLDKPLKETS
                     FYRAAIYLPSIVPAFGFSAVVTWFFHPYMGIVNAFIELLGFEGPLWLSSEAWVKPTIV
                     LAAQWGVGSSMLIFSAAIKDIPREFYESARIDGANRFVVFKKITLPLITPTIFYYLVT
                     STIGALQIFDLPMLLSNGGPANASMSLSMYIYRHAFTYTNMGYASAMAWFLFGVTFIL
                     TIFYFVFQKKWVYYLGS"
     gene            6210..7034
                     /locus_tag="PLU28_01665"
     CDS             6210..7034
                     /locus_tag="PLU28_01665"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOG33804.1"
                     /translation="MKKIKLYIPRRIALIIITMIYLLPFFWMIGTSLKPDSELMIFPP
                     RIFPESPDWSNYAKALNKFPFFMYLKNTVIITVFNLIGVVLSSSFVAYGFSKIKWKGR
                     DFFFAVMMSTVMLPGVVTMIPVFMIYKHLGWLDTFLPLIVPAFLGGGAMNVFLVRQFF
                     NTIPNDILEAAKLDGAGELKIFFKIMLPLSKPVLALISLFTFTGSWNNYMGPLIYITS
                     ESKYPLALGMPMFMSRYGTFWNQAMAASALTILPTIIFFFFAQKYLIEGIKLSGLK"
     gene            7049..8023
                     /locus_tag="PLU28_01670"
     CDS             7049..8023
                     /locus_tag="PLU28_01670"
                     /inference="COORDINATES: protein motif:HMM:NF015971.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HOG33805.1"
                     /translation="MIADFSMKPNFPWEDKPAGSDALLWRYSKNPIIKRDQAKAANSI
                     FNSAVVQYKNGFAGVFRVDDTNRRMNIRSGFSDDGINWKISDEPIRFINETDDLIESE
                     YKYDPRVVFIDDRYWITWCNGYHGPTIGIGYTYDFKSFYQIENAFVPFNRNGVLFPRK
                     INGKFCMLNRPSDNGHTPFGDIFFSESPDMVYWGKHRFVMSPGYSPWQSLKIGAGPCP
                     IETSEGWLIIYHGVLLSCNGYVYSFGAALTDLEKPWKVIKRSSSYLLSPQQLYECVGD
                     VQNVVFPVAALNEKESGRIAIYYGCADTVVGLCFGYVEQILEWLKHNN"
     gene            8102..9145
                     /locus_tag="PLU28_01675"
     CDS             8102..9145
                     /locus_tag="PLU28_01675"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOG33806.1"
                     /translation="MIPLYLQITNYVEDLILSGKYLPGDKIPSENELAEKFNTTRTTV
                     RKALDDLEKKGLLTKIFGVGTFVTEINIVSQKKIGIIVHNSQIVYGIIKFCSTIGSKC
                     FIAEHTPGLAQEKKAISELLSMGVDGIIMEPTVISLADDLLKELIKENFPVVFVDRSI
                     DMGFPVPTVITDNSQGGRLLGEHMRKVHRIESAIFVTSEDLNISSVNERYEGIKTGLR
                     FSPTIIKVPVIDGDYSNLITHLKKEHEQAIFFCNDMMAVRGYSFLLNKGFNLPKDVKI
                     IGFDNDYVSKILEPKLTTVKQNLPQLGEYAASIMVRLIKKEHVEDENRIPVSLIVRAS
                     CGCMENKEDIHRE"
     gene            9138..12206
                     /locus_tag="PLU28_01680"
     CDS             9138..12206
                     /locus_tag="PLU28_01680"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HOG33807.1"
                     /translation="MNKNKSNDIQHLKHMLAEVLPYCIVECQDIKEWMFNNNKINFNF
                     NWEKETSPVSFQKQILLEKCEKNYSVYMSGWFGGESLVKIDGKPFGEINVFHKEINLS
                     SFADGKEHVIQVFTVPRGLFGTKEEAVFRYARLVKYDDELKKAVIYVNNMINISQESE
                     NDSLSLKIIECTDDFLSSVYVPRDTDKYLLNILDNPSIISDVTNVWTHPEFTRPSHRD
                     NSEVKMNFFGKFPEYKNALEKLKNLFPKTGKVYVMGHAHIDYAWLWPVDETKRKIERT
                     FSNAVQLSAKYPEFIYSQSSAQMYKDIKELSPDVFEKIKKLVKEGKWEPAGGMWVESD
                     CNVPSAESLIRQFYYGQTFFEKEFGKRANNCWLPDVFGFSWILPQILRQACIKYFVTT
                     KLFWNDTNDFPYDVCRWRGIDGSDVLYYSYKNMEDGYNGKISAKSIINTWNNFRQKGE
                     LSSILLSFGYGDGGGGPTEEMCENFHPLSQAAGIPQVEYSRASDFFDRVEKEVDYSKL
                     PVWDNELYFELHRGTYTSQARTKRFHKLAENELRKAEILNTLFNCNNQNFLDSQWEVL
                     LRNEFHDILPGSSIKEVYEDTETELSQVIQKSAELSEKIISEKASDKGLALFNPASTH
                     NYIVFEHEKNMKVFFEGKELLKQKTFEGRYLYFIKEKISALDLVNLSAFHADEHSESI
                     VFTDSRLMENEFIKATINDDGTVDIYHKKTGKTVSDHGMNRLMLYKNIPYYWENWDID
                     KNYYKSGVQLKAQSIKTVEEGDIRKVIKVEYQIESSKIEQYYILYKTAQYIEVVSKVD
                     WHMRRALLKAIFPTKVLNRYAKYDIDNGYIERPTHNNTVYEKAKFEVLGHRWVDISQE
                     DFGVSVINDCKYGHYVKNSEIELSLIKSGIYPDFFADEGSHEFSYIIFPHESCSIKDV
                     SLLAEQYNKRPEVFNGSVNYEYPKIKIDSEAFKIYSLRKTDEKNIYLRIAEVLGTSGK
                     LTVNFENTPIKAVYLTNILHDIEQKLEVKENTVSFSFDPFKIFTLMIETA"
     gene            complement(12308..12817)
                     /locus_tag="PLU28_01685"
     CDS             complement(12308..12817)
                     /locus_tag="PLU28_01685"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOG33808.1"
                     /translation="MSFYLSLGSPMQSNDFDSISRIAAKGVVGHPERMLMIKTYHHGY
                     KFPGGGVKTGEDLLKALSREIKEETGFSVVNTGDEVGVVYEKRTDIYDPSKIFIMKTV
                     FIKCSINEKTFCELSLDAYEKELGFTPVFISLKEALAENERIITVNPDFDTWLFRDTQ
                     VLKRLLESF"
     gene            complement(12820..13236)
                     /locus_tag="PLU28_01690"
     CDS             complement(12820..13236)
                     /locus_tag="PLU28_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG33809.1"
                     /translation="MNKKGLIFLVCSVIALIFTAFYYFPNYYNFYILDEFKYVFNGNL
                     EASRGFEAKKDTELVFTSISGLSDMHAAIYTESGNEIVSISDLSKDTKLIRISLTPGK
                     YFFKILGQSDGQKKKISYNIAYKRFEMAWQKEYTEE"
     gene            complement(13247..13903)
                     /locus_tag="PLU28_01695"
     CDS             complement(13247..13903)
                     /locus_tag="PLU28_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG33810.1"
                     /translation="MSKDVLKAFKVIISMIQESFYIFLVVGAVIVLANTSMGYFFDSD
                     AVGLNAFLHTLKTNLAINLSGMLLLFSFVSVIVLFAQTINMLLIFSTPRKHIRYAVNI
                     YLLVLPIAIGAIVNLLLLLIDVPFGRVSLAITIISIIYFVVFYTGFLSMLGKRFGWQY
                     VVGTNILAVCFIMAGYNHIFKFIVFGEGKTTFLILAGAAIAILYFITNHLMKRSEYKY
                     "
     gene            complement(13896..14789)
                     /locus_tag="PLU28_01700"
     CDS             complement(13896..14789)
                     /locus_tag="PLU28_01700"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG33811.1"
                     /translation="MNQAIEINLLNKFYDNFHVLKDISLSIQENRIYGLIGRNGAGKT
                     TLMKTIAGQIYYKSGYIKIFGQDTRKDETAVNKICLARETLGAYYEQNTSIKVSKIFE
                     IAGTLFKNWDEQYARHLVKRLGLDTKKYYNKISKGMKTTVGIIIGLASRAEITMFDEP
                     YIGLDPVARDIFFEELNKDYMDNPRTMIISSHLIKEFETLFESIIMIDKGRILIDDTL
                     ESIEQKYFYLSGKESSIEKVLDGLKILSKEKIGGLASYAVTGKLNELDYDKLSKYDIQ
                     VSKMDIQKILVNLTKEDDADE"
     gene            complement(14786..15160)
                     /locus_tag="PLU28_01705"
     CDS             complement(14786..15160)
                     /locus_tag="PLU28_01705"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOG33812.1"
                     /translation="MKFDETSTQPIFLQVAELIEDMILQQTLKEEDQIFSTNQLAQTY
                     NINPATARKGFSILTDEGILYKKRGLGMYVSSGALTKIREKRRREFLNDFIKKLMTEA
                     EKLGIKKSELIEMIESYKEEKK"
     gene            complement(15291..16319)
                     /locus_tag="PLU28_01710"
     CDS             complement(15291..16319)
                     /locus_tag="PLU28_01710"
                     /inference="COORDINATES: protein motif:HMM:NF022427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 domain-containing protein"
                     /protein_id="HOG33813.1"
                     /translation="MEQLFYQEREIFEEVDLCRKDGLLNRESIGWARNSVINCNLSGR
                     WLRKKKWNYWYIMGSDCLFSVTVSNIDYAGMVFAYFYDRKTRRFIEKTVMTPFGMGCK
                     MPCKVNKTVTFENSLLKVKILSEGTDTHIFFEDTDFKKEKIFGEFVIKPYNGQETLNV
                     VIPWNERTFQFTSKQIGLPVEGVLTLGDKKYFFNSKDSFACLDFGRGVWPYKVNWNWA
                     SGFVKFNGRSVAVNLGAKWTDGTGMTENALFIDGKCIKLSENAVFEYDKNNFMKDWNL
                     KTTISDRVNLVFSPEYERVAKSKMLFLESEVHQMIGVFNGYVKDETGQKIAVENMSGC
                     SEEHFGKW"
     gene            complement(16354..16641)
                     /locus_tag="PLU28_01715"
     CDS             complement(16354..16641)
                     /locus_tag="PLU28_01715"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HOG33814.1"
                     /translation="MIKVVAKNYVRQNDLAQVVELYRELIELTRKEKGCIRYELYQDE
                     KDPLSIAMIEEWATKEDLERHLSSDHFLRLVPQIREHMQKQTDMGVYNRLI"
     gene            complement(16666..17178)
                     /locus_tag="PLU28_01720"
     CDS             complement(16666..17178)
                     /locus_tag="PLU28_01720"
                     /inference="COORDINATES: protein motif:HMM:NF040142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG33815.1"
                     /translation="MQIFFLPQWLTILLCFALWPVIQVSIAILCTKIPQAKLSYSSGI
                     FKSHRWEKNGSIYETLLKVKKWKKFLPDGGAVMKNGYRKKRLRNYSVCELEKFLAESC
                     RAELVHLLAILPFWIFGFFAPIEILVYMLIYALSVNLPCIIVQRYNRPRILHLMEKIK
                     LKVSDCDKIL"
     gene            complement(17182..18279)
                     /locus_tag="PLU28_01725"
     CDS             complement(17182..18279)
                     /locus_tag="PLU28_01725"
                     /inference="COORDINATES: protein motif:HMM:NF025017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOG33816.1"
                     /translation="MIFTLSLIVTGLVCGYLLFIHFPFLSETYSKKKLSNLTIIIPAR
                     NEQENLLGILRDLKQQTIEISEIICVNDCSSDNTAEIASSNGAKVINIENKPCEWIGK
                     SWACFEGAQASKSEVILFLDADVRLAPSAIEKLLCEYTKNRCPISVQPFHTVVKPYEQ
                     FSLFFNLIEIGANGVTTAIKGKNAGLFGPVIMIDRLTYFSIGGHKSVRTSIVDDIDMG
                     RVLNEKNIKYKLFLGNRDICFRMYGNGLRSLAQGWTKNYATGAVKTPLSLFFLTFLWL
                     SSLICLSIYPVYTFFTADYYAFAIYFLLYTVAVVHLLKITEKIGSFAKISIVLFPVQL
                     LFFLYIFFVSVLKKILRREVIWKGRKIKLEK"
     gene            complement(18284..>18985)
                     /locus_tag="PLU28_01730"
     CDS             complement(18284..>18985)
                     /locus_tag="PLU28_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG33817.1"
                     /translation="KSEQTYNAIINADTSESVLTLYLAVDQPKELFESVFGAHCFYTP
                     QKTGLSHIASLKNSISNTEDLSGYLSKYLELTTYEISCPVIRDNNLAPQGKTGLIIST
                     LLDYDLAKLAEQKGWYEEFKALCKDRIIDVLDKSILKGLKSKVIFGVISSPLTLEKIT
                     SNTRGAITGWAFKNNPVPSENRMRSIKKSIFTPLKDIYQAGQWTFSPSGLPISILTGK
                     LAADKVHKKLHKFQR"
BASE COUNT         6061 a         3252 c         3690 g         5982 t
ORIGIN      
        1 caaagttctt ggagaggaac tttaataact actacttttt attatacgag gtgaatacaa
       61 tgatgcagaa acaatttgaa aaaatgcgtt caggcaaagg ctttatagcg gcacttgacc
      121 agagcggagg cagtacaccc aaagcacttg cactttatgg cattacaaaa gattcatata
      181 caaccgatga agaaatgttt gatttggtac atcagatgag aactcgcatc ataacaagtc
      241 cttctttttc atcagaacat attctgggag ccatactgtt tgaacagact atggacagaa
      301 agatagaagg agtttttact gcagattatc tatgggaaaa aaagtctgtg ataccgtttc
      361 tgaaagtaga caaaggtctt gatcaggaaa atgaaggtgt tcagttgatg aaacccatca
      421 aagagcttga tctgttgcta aaaagggctg tcagcagaaa catattcgga acaaaaatgc
      481 gttcgttgat tttgaaagca gatatcaatg gcataaaaaa gattgtaagt cagcagtttg
      541 atattgcaaa aaaaataatc gatgcaggac ttattccaat aattgagcct gaagtagata
      601 ttaaaagcat tgataaaaaa gaatcagagg aaattttaaa gtctgaaata cttaagcatc
      661 ttcagcagct tggtaaagag agtcatgtaa tgcttaagct ttcgataccg acagtggatg
      721 atttttattc agagatcata aaacatccga gagtattgag ggtagttgct ctttcaggcg
      781 gatatacacg tgaggaagca aatgaaaagt tagctagaaa tcatggcctt atagccagct
      841 tttcaagagc tttgaccgag ggtttaaacg ttaagcagtc tgacaaggag tttgatgatt
      901 atcttaaagc gtcggtggaa agtatttata aggcatcaat agtataaata actgccggaa
      961 atccggcagt tatttttttc tcatatagtg tatgcaaaca aattatcagc actactcttc
     1021 tgaatgctct tcaaaataaa aaaatgcttt aaaaagtctg cttgtctttt aatcgaggtt
     1081 ataggaaaaa tatacctttg atttttatgg ttttacagtt atagtatgat ttttgaaaag
     1141 aaagaggttt atacctgtct atacaggtat aaacctcttt aaatacttaa ggatatcttt
     1201 tgaatatact atgtactgtt ataaaataaa aacgttgtgg aggaaaaata cttttattta
     1261 ctttaggagg tgttatattc atgaaaaact tttccaagtt atttttagtt ggttttatcc
     1321 tcatattttc gttagttgtt tttggaacaa gggaagtagt tttttggcac gggctttcgc
     1381 gcgatcctga taaaagcagc attgatgcaa taatcaactc gttcgaaaaa gaaaatccgg
     1441 acataaaagt aaaaatggtt ctggttcctg ctgcagaaac ggactcatcg aaattgttga
     1501 cggccgtata tgccggaacc ggtccggatc ttgtgtacat agacaggttt actgtctccc
     1561 aaagagccgc aaacaatgtc cttgagccaa tggaggatct tcttcaaaaa aacggaattg
     1621 atataaatgc tatgaaaaaa gagttctatg attttgctat agaggaatgt atttttagta
     1681 ataaaatgtg ggcactccct tttgatactg atgtaagggt gttgctgtac aatgaaaaat
     1741 tattaaacag tgcaggtctt aaagagcctc ccaaggatat atacagcatg ataaaggctg
     1801 ctgaaaagct tacaaaggaa ggaaaaagag gaagatatga tctggttggc ttcattccgt
     1861 ggtttgctca aggatggcca tacacatgga tatatgcatt tgaaggtaat atatttgata
     1921 atgcgacaga aaaatttatt tttgcaaccg atccgggaat aatagaagca tacgcatggc
     1981 agaaggaatg ggcagataga tttggatatg aaacgctcaa cgcttttagt tcactgtcaa
     2041 taggcgattt aaatcctttt actgcaggta agcttgcaat gatcgtggat ggaaactgga
     2101 caatatcagg tataaagcag tttgcaccta aagattttaa gtataaagtc ggaggaattc
     2161 ctaccaaaag cggaaaaccc gttacatggg caggaggatg gagtatagct gttccaaaag
     2221 gagccaaaaa cccttcggat gctgctaagc tggcatatta ctttgccaca aaagggcagt
     2281 tacagtatag tgttgatacg cttcatattc ctacttaccg caatgctgtc gatgagtttc
     2341 ttaaagcgga tccttcacaa aaaagtttta ttgagctgtt agaaaatgcc agaacaagac
     2401 ctcctcttcc tgttggtgca cttttatggg ataagctggt tgaagcaaga gattatgtgc
     2461 ttaccgggaa gaagagcgta gaaaaagcgc ttttggatgc acaaaacgaa gtacagaccg
     2521 aatataataa ggttatgaaa aaataggggt ggtagacatg aagagaaaat tcttagttat
     2581 ctgtattttt gtattattaa gcatagtata cgcacgtctt cctgaatctt catactggta
     2641 tcccaacgat attatagact gggagtatga agctgatgca gatgcaattt acaacagagc
     2701 atctgtcccg cttgccaata gaatggtggc acaggcttta gttccttctg ccgatgaaag
     2761 aattaaagtt atggcgctat ctataatgaa ctcatctacc agcggaatgc catcacaggg
     2821 tttgataggt tcaatgaaga catatccttt cactttttgg cagtatatag attatcttgt
     2881 tgcatgggca ggttctgcag gagaaggagt gattgtgcca cccagtgccg atgtgacaga
     2941 tgccggacat aaaaacggag ttccagttct tggtacgatt ttttttcctc ccaatgtcta
     3001 cggaggaaga agaaactggg ttgatcagct gctgcaaaaa gaagacggtc attttatagt
     3061 tgctgataag ttaattgaaa ttactaaata ctacggattt gacggatggt ttataaatca
     3121 ggagactaca ggctgtaacg agacacacgc taaacttatg aaagagttta tacagtatgt
     3181 gaagtcatcc gctccatgga tgacggtggt atggtatgat tcaatgacca gcaatggaga
     3241 tattgaatgg cagggagaag ttacagataa gaatgcattt tttatgaaag atctttccac
     3301 aaacaaaaag gtaagcgatg agatgtttgt agattttaga tggcaatcaa ccaaaagacc
     3361 tgatactgtg gttaattcga tcaagaatct tcaaaaatac ggtatagatt ccagcagtat
     3421 ttttgtaggg tttgatcttc aagcaagtgg atatcttacg gcatttaact ggcccaagct
     3481 tattgcagac aatgaaaagc tcatgctttc ccttggactg tactgtccga gctggtcata
     3541 ctatgattcc aaaactcctc aagagttttg gaataaagaa gaaagcttgt ggataggaga
     3601 gcacaaagtt cttgaatata acgctaaaaa agagttctct cagatagatt ggggagacta
     3661 taagcagtac aggtggagag ggatagccag tttcgtaaat gaaaaatcac ctttaacagg
     3721 gacatctttt tcaacctcct tctgtgctgg tcatggtaag tctttctata taaacggcga
     3781 aagaaaatct caaaaagaat ggtacaacag aagtcttacc gatgtgttcc cgacatatcg
     3841 ctggaaagtt tcaggtaaaa gtgttcctga gattactgtt gactatgatg atgctttcta
     3901 tggaggaaca tcgcttaagt tccaggggaa tataggaaat aatgaatctt cagaagttaa
     3961 tctttacgct gctaatcttg aaataaaagc tgattcttgt tttaaagcag ctataaaagc
     4021 aaaagataat gttgtagatg tattttatat tttaaagctt tctaacggaa aagagatttc
     4081 tataaaagcg gatcatcctg aaggaatgtg gaaagaaacc agtacttctc tttcagagta
     4141 catgggaaaa acgataacag aaataagtat aagagtagta ggaaaagaaa cagcacaata
     4201 ctctttaaat attggaaaga ttgctttagt acctcagagt aaaaaaatca tatcttcagt
     4261 taaaggagta cagatcgaca gtattgagtt cagagaaggg ctttatgcgc agcttctcct
     4321 gcattggcag gaaagcaagg atgctaagtt ttatgaaata tacagagttt taccagatca
     4381 gacacgtgag tttgtgtggg ctagttataa taactatacg tatatttccg agataaaaag
     4441 attcggaaag gaagatagtt cggcactgga agtagttgcg gttgatggtg gtatgaacag
     4501 aagtctgagg gagtttgttg tattcaattg gccgaattat ccagttccaa aagctgattt
     4561 tgaaataagc aatacaatag ctatgcctgg tgaaaagata agacttaaca gtctgtcctc
     4621 agaagtaaca gaacagcttg tatggatact tcccggagca actccggaaa tatcttatga
     4681 agtctcacct gaagttgtat acgaaaagga aggtatattt ccagttactc ttaaagccat
     4741 taactccgaa ggagaaagtg taaaaacagt agatccgctt attgttatca ctaaaaaagc
     4801 tgcaaacgtt aagaatatgg cattgaacaa aaatacttat gcaagctcta acgtgccctc
     4861 tgaaaaacct tccatggctg ttgacggaac tgttgaaaat aatagcaaat ggtgtgctgt
     4921 aggtgattta cctcattggc tggttattga ttttgaaaaa gaggtaatca taaacagagt
     4981 tatcgtaaag catgcacagt caggaaatga atcaccggat atgaatactc agatctacaa
     5041 gatacaggta agcaatgatg cccagcaatg gactgatgtt gtttcagtag attcaaataa
     5101 aaaaggagtt acagaagatt cgtttgcgcc tgcaaaagcc agatatttaa ggcttttcat
     5161 agaaaaaccc acgcaaggcg gagataaagc agctagaatt tatgaagtag aggtttatgg
     5221 attagaagct tattgaatga attttacatt cctccgcatg gattttccat gcggaggctt
     5281 atgtaaggag ggtttttacg gatgagaaag aataactcat taaatttttt taaaggaatt
     5341 ttttttattt ctccatggct tgtaggtttt ttgattttta cccttctgcc gtttattgcg
     5401 tctttttact acacatttac acgtttcagc ataaccacaa aggtgcaatg gacaggcaca
     5461 gaaaattatg taaatatgtt ttctgaccct cttttcttta aaagtcttat gaatacagta
     5521 tggatgacag taggtcttgt tcccataggt cttcttgttg ctcttggaac agcaatttta
     5581 ctggataaac cactaaaaga aacttctttt tacagggcag caatttatct gccttctatt
     5641 gttccggcgt ttggtttttc agcggtagtc acatggtttt tccatccgta tatgggtatt
     5701 gtaaatgcat ttattgaact tttaggattc gagggaccac tctggctcag cagtgaagcg
     5761 tgggttaagc ctacaatcgt gcttgctgct caatggggcg taggcagctc tatgcttata
     5821 ttttcagctg caattaaaga catcccaaga gaattttatg agtcagccag aatagacgga
     5881 gctaatagat ttgtcgtctt taaaaaaata acgttacctc ttataactcc gacaattttt
     5941 tattatctgg tgacttctac aataggagct ttgcaaatat ttgaccttcc tatgcttctt
     6001 tccaacggag gaccagcgaa tgcaagtatg tcactgtcga tgtatatata cagacatgcc
     6061 tttacatata caaatatggg ttatgcatct gcaatggcat ggtttttatt cggagtgaca
     6121 tttatactca ctatttttta ttttgttttt caaaaaaagt gggtttatta cctgggttca
     6181 taaattaata aagaatggtg gcgattataa tgaaaaaaat aaaactttat atacccagaa
     6241 gaattgcctt aataattatt actatgatat atcttctgcc ttttttctgg atgatcggaa
     6301 catcattaaa gccagacagt gaacttatga tttttcctcc cagaatattt cctgaatctc
     6361 ctgattggtc aaattatgcc aaagcactta ataagtttcc gttttttatg tatctcaaaa
     6421 atacagttat aattaccgta tttaatctta taggagttgt tttgtcttct tcttttgtgg
     6481 catatggatt ttcaaagatc aaatggaaag gaagggattt tttctttgcg gttatgatga
     6541 gtactgtgat gcttccgggt gttgtgacta tgatacctgt ttttatgatt tataaacatc
     6601 tcggatggct ggataccttc ctgcctttaa ttgtacctgc ttttcttggg ggcggagcta
     6661 tgaatgtttt tctggtgaga cagtttttca acactattcc caacgatata cttgaagctg
     6721 ccaaacttga tggtgcggga gagttaaaaa ttttttttaa aattatgctt ccactttcaa
     6781 aaccggttct cgcgctgatt agtctcttta ccttcacggg gtcatggaac aattatatgg
     6841 ggccgttaat ctatattaca agtgaatcaa agtatccttt ggcgctgggt atgcccatgt
     6901 ttatgagcag atacggtaca ttctggaatc aggcaatggc ggcatcggcg cttacgattc
     6961 ttccaacgat aatatttttc ttttttgcac aaaagtatct tatagaaggt attaagcttt
     7021 cgggcttaaa ataaacggga gtgattttat gatagcagat ttttccatga agcctaactt
     7081 tccctgggaa gataagcctg ccggatctga tgcattgtta tggaggtact ctaaaaatcc
     7141 tattataaaa agagatcagg caaaagcagc aaacagtatt tttaacagtg cggtcgtgca
     7201 gtataaaaac ggatttgcag gtgttttcag agttgatgat accaacagaa ggatgaatat
     7261 aagaagcggt ttcagtgatg acgggattaa ctggaaaatt tccgatgagc cgatccgttt
     7321 tattaacgaa acggatgatt taatagaaag tgagtacaaa tatgacccta gagttgtttt
     7381 tatagatgac cggtactgga taacctggtg caacggttat catggtccta ctatagggat
     7441 tggatatacc tatgacttta aaagttttta tcagattgaa aatgcatttg tgccattcaa
     7501 cagaaacggg gtactttttc caagaaagat taacggaaaa ttttgtatgc taaacagacc
     7561 atctgataat ggtcatactc cctttggaga tatctttttt agcgaaagtc ctgatatggt
     7621 ttattggggg aagcaccgtt ttgtaatgtc tcctggttat tcgccgtggc agtctttaaa
     7681 gataggtgca ggcccttgtc ctatagaaac atcggaagga tggctgatta tttatcatgg
     7741 agtccttctt tcatgcaatg gatatgttta cagtttcgga gcggctctta ctgatctgga
     7801 aaaaccgtgg aaagtaatca aaagatcatc ttcgtatctt ctttcccctc agcagctcta
     7861 tgaatgtgtt ggggatgtcc agaatgttgt ttttcctgtt gctgctctta atgaaaaaga
     7921 aagcggaagg atagctattt attatggatg tgcagataca gttgtagggt tgtgttttgg
     7981 atatgttgag cagatattgg aatggcttaa gcacaacaac taataagaag tgtatttttt
     8041 atagcaaaat gcgataaaat aaagtaattt aagaaatttt atcttttcag gaggtatcct
     8101 aatgattccg ctgtatctgc aaataacaaa ttatgttgag gatcttatcc tttcaggcaa
     8161 gtatcttccc ggggataaaa ttccttctga aaatgagctt gctgagaagt ttaatactac
     8221 aagaaccacc gtaagaaagg cgctagatga tttagaaaag aagggtttgc ttacaaaaat
     8281 tttcggtgta ggaacttttg ttactgaaat caatatagta tcgcagaaga aaataggaat
     8341 aatagtacat aattcacaaa ttgtttatgg aataataaaa ttttgctcca ctatagggag
     8401 caagtgtttt atagcagaac atacaccagg gcttgcccag gagaaaaagg ctataagtga
     8461 gcttttatca atgggggtag acggtataat aatggagccg acagttattt cattggccga
     8521 tgatctgctt aaggagctca ttaaagagaa ctttcctgtt gtttttgttg acaggagcat
     8581 tgatatgggt tttcctgttc cgactgttat tacggacaac agccagggtg ggagactttt
     8641 aggtgaacat atgaggaaag ttcataggat tgagtcggct atttttgtta ccagtgaaga
     8701 tcttaatatt tccagtgtaa atgaaaggta tgaagggata aaaaccgggc tcagattttc
     8761 tcccaccata ataaaagtac cggtgataga cggggattat agcaatctta taactcatct
     8821 gaagaaagaa catgaacagg ctattttctt ttgcaatgac atgatggccg taagaggata
     8881 ctcattcctt ctgaataaag ggtttaatct ccccaaggat gttaaaataa tagggtttga
     8941 taatgattat gtttcgaaaa tccttgaacc taaactcaca acagttaagc agaatcttcc
     9001 gcagcttgga gagtatgctg cttcgataat ggttagactt attaagaaag aacatgtaga
     9061 agatgaaaac agaataccgg tttcgcttat agtaagagca tcatgtggtt gtatggagaa
     9121 taaggaggat atccatcgtg aataaaaaca aaagcaacga tatacagcat ttaaagcata
     9181 tgcttgcaga agtacttcct tactgcatag tggagtgcca ggatataaaa gaatggatgt
     9241 ttaacaacaa taagattaat tttaatttta attgggaaaa ggaaacctct ccagtaagct
     9301 ttcaaaaaca gattctcctg gaaaaatgtg aaaaaaatta ctctgtctat atgagcgggt
     9361 ggtttggagg agaaagcctt gtaaaaattg acggaaaacc ttttggagag ataaatgttt
     9421 ttcacaaaga aattaatctg tcttcttttg ctgacgggaa ggagcatgta atacaggttt
     9481 ttaccgtacc cagaggtctt ttcgggacaa aagaggaagc tgtcttcaga tatgcccgtc
     9541 ttgtgaaata cgacgatgag cttaagaagg ctgtcatata tgtaaacaat atgatcaata
     9601 tttctcagga aagcgaaaat gatagtcttt cattaaaaat aattgaatgt accgatgatt
     9661 ttctttcaag tgtttatgtt cccagagata cggataagta ccttctcaat atcctggata
     9721 atccttcaat aatttctgat gtaacaaatg tatggacaca tcctgagttt acaagacctt
     9781 ctcacagaga taattcagag gtcaaaatga acttttttgg aaaatttccg gagtataaaa
     9841 acgctttgga aaaactaaaa aatctgtttc caaagacagg aaaagtttat gttatgggac
     9901 atgcgcatat agactatgcg tggttgtggc cggttgacga aactaaaagg aagattgaaa
     9961 gaaccttttc aaatgctgtc caactttcag cgaagtatcc ggagtttatc tacagccagt
    10021 cttcagctca gatgtataaa gacataaaag agctttctcc ggatgttttt gagaagataa
    10081 aaaaacttgt gaaagaagga aaatgggagc ctgcaggagg tatgtgggtg gaatcagatt
    10141 gcaatgttcc ttctgcagaa tctttgatca gacagtttta ttatggtcag acattctttg
    10201 aaaaagagtt tgggaaaaga gccaataact gttggcttcc ggatgttttc ggcttttcat
    10261 ggatacttcc ccagatactt aggcaggcct gtataaagta ttttgtaacc actaagcttt
    10321 tctggaacga tacaaatgac tttccatatg atgtgtgcag gtggagggga atcgacggat
    10381 cagatgttct gtattacagt tataagaata tggaagatgg atataacggc aagataagtg
    10441 caaagtcaat aattaataca tggaataatt ttagacagaa aggggaactc tcatcaattc
    10501 tactttcttt cggatatggc gatggaggag gaggcccgac ggaagaaatg tgcgaaaatt
    10561 ttcatccgct ttcccaggcg gctggtatac cccaagtaga gtactcaaga gcatcagatt
    10621 tttttgacag ggtagaaaag gaagtagatt attcaaaact tccggtttgg gataatgagc
    10681 tttactttga gctgcatcgc ggaacctaca cctcacaggc cagaaccaaa agatttcaca
    10741 aactagcgga gaacgaacta agaaaagccg aaattctaaa tactttattt aactgtaata
    10801 atcaaaattt tttggatagt cagtgggaag tgcttctcag aaatgaattt cacgatattc
    10861 ttccgggatc gtcaatcaaa gaagtgtatg aagataccga aacagagctt agtcaagtaa
    10921 tacaaaaatc agcagagcta tctgaaaaaa taatttctga aaaagcttct gataaaggtc
    10981 ttgctttgtt caatccggca agtacccata attatatagt ttttgagcat gaaaagaata
    11041 tgaaagtatt ttttgaaggt aaagagcttt taaaacagaa aacttttgaa ggaagatacc
    11101 tgtactttat aaaagaaaaa atctctgctc tggatttggt taatctgagt gcttttcatg
    11161 cagatgagca tagcgagagt atagttttta cagatagcag actgatggaa aatgagttta
    11221 taaaagccac gataaatgat gacggaacgg ttgatatata ccataaaaaa accgggaaaa
    11281 cagtttctga ccatggaatg aacagactta tgctgtacaa aaacataccg tattactggg
    11341 aaaactggga tattgataaa aattactata agtcgggagt tcaattaaag gcacaaagca
    11401 taaaaacagt tgaagaagga gatataagaa aagtaatcaa ggttgagtac cagatagaaa
    11461 gttctaaaat agaacagtac tacatactat ataaaacagc ccagtatatt gaagttgttt
    11521 ccaaagttga ctggcacatg agaagagcgc ttcttaaggc catttttcca acaaaggtgc
    11581 tcaacagata tgcaaagtac gatattgata acggttatat tgaaaggcct acccacaaca
    11641 ataccgttta tgaaaaagca aagtttgaag tactcggtca cagatgggtt gatatatcgc
    11701 aggaggattt tggagtatca gtcataaacg actgtaaata tgggcattat gttaagaact
    11761 ccgagatcga actgtcttta atcaaatctg gcatatatcc tgattttttt gcagatgagg
    11821 gcagtcatga gttttcatat attatattcc cccatgagag ctgctcgatt aaagacgtat
    11881 cgttgttagc cgaacagtat aacaaaaggc cggaggtttt taacggaagt gtcaattatg
    11941 agtatcctaa aataaaaata gactctgaag cttttaagat ttattcattg aggaaaactg
    12001 atgaaaaaaa tatttatctc agaatcgctg aggtacttgg aacatctgga aaacttacag
    12061 ttaactttga gaatactcct ataaaagcag tatatctaac caatattctt catgacatag
    12121 agcaaaaact tgaggtaaaa gaaaatactg tcagtttcag ttttgaccct ttcaaaattt
    12181 ttactctgat gattgagaca gcgtagcttt ttctctttca catattttta tattaacaaa
    12241 agccaggctt atgattgcct ggcttttgtt aatatgttct cagtattttg tttgtagagc
    12301 agctgcttta aaagctttcc aaaagtcgtt tgagcacctg ggtgtctctg aaaagccacg
    12361 tatcaaaatc gggatttacc gttataatac gttcgttttc tgcgagagct tcttttaagc
    12421 ttataaaaac aggggtaaaa cctagttctt tttcgtacgc atctaaagat aattcacaga
    12481 aggttttttc attgatactg cactttatga atacagtttt cataataaaa atttttgaag
    12541 gatcgtagat atcggtgcgc ttttcataaa caactcctac ttcatcaccc gtgtttacaa
    12601 cagagaagcc cgtttcttct tttatttccc tagataaagc ttttaaaagg tcttctccgg
    12661 tcttaactcc tccgccgggg aatttataac cgtgatggta agtttttatc ataagcattc
    12721 tttccggatg tcctactact ccttttgctg cgattctgga gatactatca aaatcattac
    12781 tttgcattgg agaaccaagg cttagataaa agctcatggt tattcctcgg tatactcttt
    12841 ctgccaagcc atctcaaatc ttttataggc aatattatag gatatttttt tcttttgacc
    12901 gtcactctgt cctaagattt taaaaaaata cttgccagga gtcaaagaga tcctgatgag
    12961 cttagtatct tttgatagat ctgaaatgct gactatttca tttccggatt ctgtgtatat
    13021 ggctgcgtgc atatcagata agccggatat tgaagtaaat accagctcag tatctttttt
    13081 tgcttcaaat cccctagatg cctcaagatt tccgttgaat acatacttaa attcatcaag
    13141 tatataaaaa ttatagtaat ttgggaagta gtaaaatgct gtaaatataa gagctattac
    13201 gctgcataca agaaagatca atcctttttt attcatttta ttcctcctag tatttatact
    13261 ctgaacgctt cataagatga ttggtaataa agtacaaaat tgcaatagct gcacctgcaa
    13321 gtataagaaa agttgttttg ccttcaccaa aaacaataaa tttgaaaata tggttataac
    13381 ctgccattat aaagcaaacc gcaagaatat ttgttcccac cacatactgc cacccaaatc
    13441 tttttccgag catggaaagg aaaccggtgt aaaaaactac aaagtatatt atggaaatta
    13501 tggttatggc cagactaact ctcccaaaag gaacatcaat aagtaaaagc aatagattaa
    13561 ctatggctcc aattgcaata ggaagaacaa gaagatatat atttactgca tatcttatat
    13621 gtttacgcgg ggtagaaaag atcagcagca tgttgattgt ctgtgcaaat agaacaatta
    13681 ctgaaacaaa agaaaaaagc aagagcattc cagaaagatt tattgccaga ttagttttca
    13741 aagtgtgtaa aaaagcgttc agaccgactg catcagagtc gaaaaaatat cccatagatg
    13801 tatttgcaag aactataacc gctccaacca cgagaaatat ataaaaactt tcttgtatca
    13861 tgcttatgat gactttaaaa gcctttaaaa catctttact catctgcgtc gtcctcctta
    13921 gtcaggttca caagtatttt ctgtatatcc atctttgata cctgtatatc gtactttgac
    13981 aatttatcat aatcaagctc attgagtttt ccagttacag cataacttgc aagtcctccg
    14041 attttttcct tcgaaagaat cttcagtccg tcaagaacct tttcaatgct gctttctttt
    14101 ccgctcaggt aaaagtactt ctgctctatg ctttcgagcg tatcatcaat tagtattctg
    14161 cccttgtcaa tcattataat gctttcaaaa agtgtttcaa actcttttat gagatgtgag
    14221 gatattatca tggtacgagg attgtccata taatctttgt tgagctcttc aaagaatata
    14281 tctcttgcca caggatccag accgatgtat ggttcatcaa acatcgttat ctctgcccgg
    14341 cttgcaagac cgattattat gcctaccgtg gttttcatgc ccttggagat tttgttgtaa
    14401 tattttttag tgtcaagacc gagtcttttt acaagatgcc tggcatactg ttcatcccag
    14461 ttcttaaaaa gagtccctgc tatttcaaat atctttgata cctttataga agtattctgt
    14521 tcatagtatg ctcctagggt ttcacgggca aggcagatct tatttactgc tgtttcgtct
    14581 ttacgtgtgt cctgaccgaa aatttttata taaccggatt tataatatat ctgacctgca
    14641 attgttttca taagagttgt ctttcctgcg ccgtttctgc caatcaatcc gtatattctg
    14701 ttttcctgta tagaaaggga aatatctttc agtacatgga agttatcgta aaatttatta
    14761 agaagattaa tttctattgc ctgattcatt ttttctcctc cttgtagctt tctatcattt
    14821 caataagctc tgattttttt attccaagtt tttctgcttc agtcataagt ttttttataa
    14881 agtcgttcaa gaactctctt ctcctttttt ctcttatttt tgtcagtgcc cccgaactta
    14941 cgtacattcc aagacctctt ttcttgtaga gaatcccttc atccgtgagg attgaaaacc
    15001 cttttcttgc tgtggcggga tttatattgt aggtctgcgc aagctggttg gttgaaaaga
    15061 tctggtcctc ttctttaagc gtctgctgaa gaatcatatc ttcaatgagc tccgctacct
    15121 gcaagaatat gggctgagtt gatgtttcat cgaatttcat ctgttcacct ccgtttctta
    15181 gttagctact caactaacta agtacatgag tattgtacta ttttcttttg attttgtcaa
    15241 gcaaaaaaat gtcttttgaa aaaaatcttc aaaagacata attactgaag ctaccacttt
    15301 ccaaaatgtt cttcggaaca gccgctcata ttctctacag cgattttctg ccctgtttcg
    15361 tcttttacat atccgttgaa aacaccgatc atctgatgga cttcagattc aagaaaaagc
    15421 attttcgatt tggctactct ttcatactca ggagaaaaaa caagattaac cctgtccgat
    15481 atggttgtct taaggttcca atctttcata aaattatttt tatcgtactc aaaaacagca
    15541 ttttcactca gttttataca tttaccatca ataaaaagtg cattttctgt cattcctgtg
    15601 ccgtcggtcc attttgctcc aagattaaca gccacagatc tgccattaaa ctttacgaaa
    15661 ccgcttgccc aattccaatt tactttgtat ggccatactc cccttccaaa gtcaaggcac
    15721 gcaaaagaat cctttgagtt gaaaaaatat tttttgtctc ccaaggtaag aactccttct
    15781 acaggtagac ctatttgttt ggatgtaaac tgaaaagttc tttcattcca tggaataact
    15841 acattaagtg tttcttggcc gttataaggt tttatgacaa actcgccaaa tattttttct
    15901 tttttaaaat cagtatcttc gaaaaaaata tgtgtatctg ttccttctga aagtattttt
    15961 actttaagta aagaattttc aaaagtaaca gttttattga ctttacaagg cattttacag
    16021 cccattccaa aaggagtcat aacagttttc tctataaacc gtcgtgtttt tctatcgtaa
    16081 aaataagcaa aaaccattcc ggcgtaatca atattggata ctgtaaccga aaaaagacag
    16141 tctgaaccca tgatatacca gtagttccat ttctttttcc gtagccatct gccggaaaga
    16201 ttgcagttga taactgaatt tcttgcccat cctatacttt ccctgttaag taaaccatct
    16261 tttctacata agtctacttc ttcaaaaatc tctctttcct gataaaaaag ctgttccatc
    16321 ttcttccccc ctgtaatgaa tctaacctaa taatcatata agcctattat aaactcccat
    16381 atctgtctgc ttttgcatat gctctcttat ttgaggtaca agtcttaaga aatggtctga
    16441 ggataagtgt ctttcaagat cttctttcgt tgcccattct tctatcatag ctatgcttaa
    16501 aggatccttt tcatcctgat aaagctcgta cctgatacag cccttttctt tcctggtaag
    16561 ttctataagt tccctataaa gttccacaac ttgtgctaaa tcgttttgac gaacgtaatt
    16621 ttttgcaact acctttatca tagtagcacc tctttttcct ttcttttaca gtattttatc
    16681 acaatcagaa actttaagct ttattttttc catcaaatgc aatattcttg gtctgttata
    16741 tctctgcaca attatgcagg gtaaatttac agaaagggca taaattaaca tatataccag
    16801 tatctctata ggagcgaaaa aaccaaaaat ccagaacgga agaattgcaa gaaggtgaac
    16861 cagttcagcc ctgcaagact cggctaaaaa tttttcaagt tcgcaaacag aataattccg
    16921 aagtcttttt ttccggtacc cgtttttcat tactgctcca ccgtcaggta agaacttttt
    16981 ccattttttt acttttaata atgtctcgta aatggaaccg tttttttccc accggtgaga
    17041 cttaaaaatt ccggatgaat atgagagctt tgcttgtggt atttttgtgc acaggatggc
    17101 tatggaaact tgaattacag gccacaaagc aaagcacaaa agtatagtaa gccattgcgg
    17161 aagaaagaag atctgcataa atcacttctc cagttttatt tttctgcctt tccatatcac
    17221 ttcacggcgc agtatttttt tcagaacaga gacaaaaaat atataaagaa aaaaaagaag
    17281 ttgaacaggg aataaaacaa ttgatatttt agcaaatgaa ccgatctttt ctgtaatttt
    17341 taacagatga acaacagcta cagtataaag caaaaaatag atggcaaaag cataataatc
    17401 tgccgtaaaa aatgtatata cagggtatat actcagacat atcagcgaag aaagccacag
    17461 aaaagtcaag aaaaataagc ttaaaggggt tttaactgca cctgttgcat aattttttgt
    17521 ccacccttgg gctaaagatc taagaccgtt gccgtacatt ctgaaacaga tatcccggtt
    17581 tccaagaaaa agcttgtact ttatattttt ttcattgagt actcttccca tgtcaatatc
    17641 atctacaatg cttgtacgta cgcttttatg tcctccgata ctaaaataag ttagacggtc
    17701 aatcataatc acaggtccaa aaagaccggc atttttgcct ttaatagctg tcgtaacacc
    17761 atttgctcca atctcaataa gattaaaaaa taaagaaaac tgttcataag gttttacgac
    17821 agtatgaaaa ggctgaacag aaatagggca tctgtttttc gtatactcgc atagcagttt
    17881 ttctattgct gatggggcaa gtcttacatc tgcatccaaa aaaagtatga cttcactttt
    17941 tgaagcttgg gctccttcaa aacatgccca ggattttcca atccattcac atggtttatt
    18001 ttcaatatta atgacttttg ctccgttact acttgcaatt tcggcggtat tatcggaaga
    18061 acagtcattt acacagatta tttctgaaat ttcaattgtc tgctgtttaa gatctctcag
    18121 tatgcctaaa agattttcct gttcgtttct tgcgggaatt attattgtaa gattagataa
    18181 tttttttttg ctgtaagttt cactaagaaa aggaaaatga atgaacagca gataaccgca
    18241 tactaatcct gtcactataa gacttaatgt aaaaatcatg acgtcacctc tgaaacttgt
    18301 gtaacttctt atgaacttta tcagcagcaa gttttcctgt aagaatggat ataggaagtc
    18361 cggatgggct gaaagtccat tgtccggcct gataaatatc cttcaagggg gtaaaaatag
    18421 attttttgat acttcgcatc ctgttttctg aaggtacagg attgtttttg aatgcccagc
    18481 ctgttatcgc ccctctggta ttagaagtta tcttttccag tgtgagcgga gaggatataa
    18541 ctccaaaaat tactttggat tttaaccctt ttaaaatact tttatccagc acatcaataa
    18601 ttctgtcttt acataacgct ttaaactctt cataccatcc tttctgctct gcaagtttgg
    18661 ctagatcgta gtcaagaaga gtgcttatta tcagacctgt tttaccttgt ggagcaagat
    18721 tgttatcacg tataacagga catgatatct cgtatgtggt caactcaaga tacttggata
    18781 aataaccgga aagatcttcg gtattgctta tgctgttttt tagagaggca atatgagaaa
    18841 gacctgtttt ttgtggtgtg tagaagcaat gggctccaaa aacactttca aacaattctt
    18901 ttggctgatc aaccgctaga taaagtgtaa gtacagattc cgaagtatca gcatttataa
    18961 tcgcattata ggtttgctcc gattt
//
LOCUS       DAOSHR010000017        18916 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_2367_length_18916_cov_5.680240, whole genome shotgun sequence.
ACCESSION   DAOSHR010000017 DAOSHR010000000
VERSION     DAOSHR010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 18916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18916
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2367_length_18916_cov_5.680240"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2434)
                     /locus_tag="PLU28_01735"
     CDS             complement(<1..2434)
                     /locus_tag="PLU28_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG33818.1"
                     /translation="MKNAYLVTVLFIIVFLFSACTSKSSAFQPINSKNISISDSDHGA
                     VLSTSRDIYGIQLHIKTPEGTNREDLVFSGDTIHIMQQINPAEYKVVLAGKNGSIKSS
                     EPFLTIKKAKSKDIHIINEELPLSEKALTVRSFIDRNDTDYSKYGLYIKKSFTLKGDE
                     LLCDLYAVNRKGKEKLYAFGLEFTFASTALTLNDFTFSPSINTDFISLKSDASSMPFI
                     IASSARSKEKALDFTSPLKIGTFRFSVIGENLAAALDINVDSLKVIDTLDSSIKDLPV
                     IVPAKGTPVVMIQSFVGDFNKDGSIDGTDLSIFQAAYFANNTAVDIYPAVDSDKDGFY
                     DISTGDMKITVDDLIVFAANYSKKMGFPTYPYPENLAVKTPLDTKLKWEYFGTASPSS
                     LRIHYGNSISSMTNEVPSSDIDLVSKTATLPLLSEDKSYYWQVSAAYPAGEVHSEIWK
                     FSTNGLPKISVNRLGSKEGTAIPSGLTDVAISNIPIYWSAPDSDGDTVTCTIKLFNSS
                     DYLESSEISSAFSETTQSSATIKGPLLYNKDYFIQIRAEDSSLGVSVLGNAASPYFFR
                     TKEGISLDSSTFPLKKAYVSARFNGLYEVDYSALISSGTVSSSCAFNTPGLFPIVESE
                     YFQNNKTDPQYMISVIGNYNKDPKFYIGQSHVLGAFDTIPASFTGLKEEWSVCALTME
                     SEGATKLFIGTSLNIYKFPDITDPSNFKKLKVPGIDDMEKKGNYLYLACKSANKLLVV
                     DTSGDMMIKASKAIGSPIDLKVNGDFLYVLTEGKTLFKYSIVSPELLIDKGNITSVGG
                     LSAVYKLAVYSDS"
     gene            complement(2448..2858)
                     /locus_tag="PLU28_01740"
     CDS             complement(2448..2858)
                     /locus_tag="PLU28_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HOG33819.1"
                     /translation="MYTKELSVFVDEEFCYTVFIKNFPNIKSFSILIDFDKNYLAPVF
                     VDERFYYTLSPLADSSLVFTKHSYNSLVVGVTMTEAFSAGAEEMVLLVFKFKAKSPGD
                     TAIHIVQTDTTFFSENLNTPFSGYSVINSSIHIR"
     gene            complement(2968..4140)
                     /gene="flgL"
                     /locus_tag="PLU28_01745"
     CDS             complement(2968..4140)
                     /gene="flgL"
                     /locus_tag="PLU28_01745"
                     /inference="COORDINATES: protein motif:HMM:TIGR02550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HOG33820.1"
                     /translation="MRITEIMINNSALSDLQSTMRKFSKLNEQLTSGKKVNFPSDNAA
                     IASRMSNLDSRLREIERYQSNASIVENFSKMYDQILQDVSSVYTRVKSLAVQGANSGT
                     LDSNLDRKSIADELEKINDHLISLANTSVSGKFIFGGAESDKTPVSDSGDIMTDPKAN
                     IKHKISVGGYELEYGVTVYDAFGTKSGTSIFKIVDRLKDAFIKSDEITKDLKDAIKTK
                     DNLSMQNVTLKNEIKEIEKRKLQAEAAGNTALAASLQTQIDEKNSSIATNETKIKELQ
                     GDPSAATPVKGLIESKSELLKKAAESIQNELGAIDEIQESSSRTISQVGGTQRLLELT
                     NSRMENFKTFSEEFLATETDADPAKVYTDMAMQKSVMDAALKALAQIVPKTILDFM"
     gene            complement(4151..7054)
                     /gene="flgK"
                     /locus_tag="PLU28_01750"
     CDS             complement(4151..7054)
                     /gene="flgK"
                     /locus_tag="PLU28_01750"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HOG33821.1"
                     /translation="MPDVSLYGLLNTGLLGVYTHKIAMSVVGHNIANTNTQGFSRQRP
                     LIVTTSPIPVNSLTQPSIPLQFGTGSKVTDIQRVRDEFLDVQYRQVTNRYNYWDTLNG
                     NMHYIEQLFGEPGETGLRTFFDSFWSSMEEIKTDPTNEAAKGQIVSRATELKNVLYDI
                     DYRLEQLQIDLNDEIKTRVDQLNSAFKQIAYLNDKISSSQVMGSSPNDLLDERDRLLD
                     QISELSNVRVNYDKDFQMSISVGDRTVLNGKTFIPLKVERVPGEQYKYAVFSNNTQIS
                     FNDGKMKAIMELRDSVIKDYRSKLDEFALTLNDTVNLVYQEGWDSTGNTTGARFFKDM
                     SAKLDSDNARLYRIASSCFFNGGPVNHIPGSRSFNGIDFQNYEINMITKGQIVSVGED
                     FTNTDSEKLSLGTKEKISTIADGLKSLEMSLAYNKNKDLTTLNNYGGDSLNNRLIIDF
                     NSNVFNQLGLKTKAIEAIKLNKAESGQLILNKGSSKQEVVNISVGTGEGIEKVAADIN
                     AQSSMIKAFTLNDSLYIVPTKNVEDFNINNVVFDDKDGIMTKMGAAKTRLNVLDTSEP
                     TLQNVFNMSVFRTKTYDVKSFDAASSFEVSMEYADGTVSSIPLTDLGLFTEDSIGKSG
                     EFKVRKNGDKIEIIPTKSDFNFEQIIDLQVKVNGTTLYSKDKLFDLQSAKEFFAAEGM
                     DYNALMNDPEITWKSDFIKYGINLEINGININIDPTRDTFQSMIDKINSTGSGLSAFQ
                     TPNGRFALKANSSVNFDMRNITIKGHEKFFELLGLKNSSEPPYKTNYVFMGPDRTARE
                     LEVDLQNSSVLKLDKNVSLVRQLEIVDTLKSNPSSLSIDLGKGIDSNGDWKADYFIPI
                     GKHSTTVWNYLSKIKELPLLSEGKVGFSGYLASLVAEMGISGENASKMKTNSQYLKEQ
                     IDGERERVKGVSIDEEVSNMIKYQQAFNASARVITAADEMINKVVNGLGLVGR"
     gene            complement(7059..7544)
                     /locus_tag="PLU28_01755"
     CDS             complement(7059..7544)
                     /locus_tag="PLU28_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG33822.1"
                     /translation="MKEENKGLVDILDKEFVLIDRLIIKVDSFRQSLIKKESTEYINS
                     LLAEIDLLSKEFQEADIKRDQFVADISSCHNIPNAIKDIVDFFSVTDKDVALKLAGFV
                     EKMQNLTFTLDCLHQALDFESNYNNLLLNMLNIDKTGKSTYAKSGYSKNNYQNKLNWE
                     G"
     gene            complement(7541..7810)
                     /locus_tag="PLU28_01760"
     CDS             complement(7541..7810)
                     /locus_tag="PLU28_01760"
                     /inference="COORDINATES: protein motif:HMM:NF016223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HOG33823.1"
                     /translation="MEIRGLGSPNSSQLKQIKKDPVKDIKSSSENDFSKEFEKITGDS
                     MKYIELSRKIPNVRNHLVQSYKNSINEGTYSINISEIVKKMLGEI"
     gene            7994..8557
                     /gene="frr"
                     /locus_tag="PLU28_01765"
     CDS             7994..8557
                     /gene="frr"
                     /locus_tag="PLU28_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOG33824.1"
                     /translation="MPAKTAYGKTVQEKLEKTFKHLEEELKGLRTGRPSTTIFENIKV
                     EFYGNPSPINQVGSLNLTEDRTLVITPYDRSMLSKIEKAINASDIGLHAINDGIVIRV
                     SFPTPTGEDRKKWVKKAKEQVEETKIALRNIRRDDLKKIKDDQKNSKITEDESKKAEE
                     DITKVLKEYEDKCDLLFTKKEKEIMES"
     gene            8554..9249
                     /gene="uppS"
                     /locus_tag="PLU28_01770"
     CDS             8554..9249
                     /gene="uppS"
                     /locus_tag="PLU28_01770"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HOG33825.1"
                     /translation="MTGLKLPEHVAIIMDGNGRWAIGKGLSRNLGHEKGAQVAEEVIQ
                     WSADIGIKYLTLYSFSYENWKRPKEEVNYLFDLFVRYLESRMGKIVSKGVKMRFSGRI
                     NELPDSLIKAIRNIETKSAGNTKLQLIMALNYGGRQEIADAVNKALNNGKKDISEEDI
                     AKNLYLADVPDPDLIIRTSAEERISNFLLWQSAYSELYFSKVLWPDFSYEEYIRALES
                     YSKRQRRYGGTGS"
     gene            9255..10103
                     /locus_tag="PLU28_01775"
     CDS             9255..10103
                     /locus_tag="PLU28_01775"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOG33826.1"
                     /translation="MPVNKKDLAFRTLSGVIIGPIVVFSFLTYQSLLGLVTTIIILAS
                     YEFIEMSNKEIKNNFIKLLYAFVIGVASLLYGFSLKTEYAGILPFEAEIIFFIAFVFI
                     ASVSLFTAKDQNEAKNLITSSVFAIMYISFFLSIFYNIHIGYGGTMGILALTSVWAYD
                     ACAYFFGLNFGKHKLSPKFSPKKTWEGFFGGVLGTFVYILIYEFFRELYGFAKLDIFY
                     ALIFAVMVACFDTVGDLTESIFKRFYGVKDSGEVLPGHGGMLDRIDGLLVVTPMWYLF
                     LRILGI"
     gene            10119..10526
                     /locus_tag="PLU28_01780"
     CDS             10119..10526
                     /locus_tag="PLU28_01780"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOG33827.1"
                     /translation="MKKFTKFREGFTVLEVVVVLVIIAIITAAAIPGFSNMVSQAKVT
                     KAINDMRTVQTAVVQYLIYNSDTSDISLEKLYDGGFIGSKPLDISVSSALSDSEIHIV
                     YTKADPKHDKFIKVDPNIEKDSDEKPMLRIMKN"
     gene            10631..13249
                     /gene="alaS"
                     /locus_tag="PLU28_01785"
     CDS             10631..13249
                     /gene="alaS"
                     /locus_tag="PLU28_01785"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOG33828.1"
                     /translation="MSWMSADEIRERFLAFFESKGHKRLASSPLVPNDPQLLFTVAGM
                     VPFKPIFWGKVEPTYTRVTTCQKCIRTNDIENVGRTPRHHTFFEMLGNFSFGDYFKNE
                     SIEWAWEFLTKELKIDPDRLWPSVYLEDDEAYRIWRDKIGVKESKIMRFDKKENWWGP
                     VGPTGPCGPCSEIYIDTGNKSKCEDPQNCSPACECGRFVEIWNIVFTEYYQDENGKLE
                     PLARKNIDTGAGLERISAAMQGVYNNFDSDLFQPTIKVIEKSFGIKYGESEKADVSIK
                     VIADHTRAVTFTVAEGVLPSNEGRGYVLRRVLRRALRHGNLLGKKEPFMSEIIDSLVE
                     RMEKIYPELSEKKEFVKKIVKAEEDRFLQTLEKGIDKLNDITNALSDKTIPGAVAFEL
                     YDTYGFPIDITIEIAQEKGFDVDVQSFETFMKEQRERARNAAGEKEYDKANQAYKIVG
                     EELKNTVFTGYEKNHDVQKLLYIIKGSELSETVSEGEQAELIFASTPFYAEKGGQISD
                     TGEILSESAVALVDEVKVIYNEVVSHKVSVTKGTIKKGDILSLHINEENRAAIKRNHT
                     ATHLLHSALRIVAGSHVKQAGSFVDSKRLRFDFTHFQALSQEQIAQIERIVNTEILKA
                     YPVITEIKSLEETKNENVIALFQEKYGNTVRVVKAGGFSSELCGGTHVSNTGEIGLFK
                     IISEVSVSSGIRRIEAFTGINSYEYVNELSKKVTNTSKILDASSENIEEKAFQTLELT
                     RNLTKEIKRLEEKLAGQSLDGFIKNARMINNIKVICGVFENVEPDVLRNSIDIIAQKE
                     GEALIVLFSRSDKVIFCVKVPSFMTKAHNAGGIAKKIAQFLGGGGGGKPDFAQAGGKD
                     IAKVAQVISEIERFIF"
     gene            13513..14529
                     /gene="ord"
                     /locus_tag="PLU28_01790"
     CDS             13513..14529
                     /gene="ord"
                     /locus_tag="PLU28_01790"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,4-diaminopentanoate dehydrogenase"
                     /protein_id="HOG33829.1"
                     /translation="MYRVAVWGFGAMGSGIARNIMTKYDLSLVGVYDSREDFMGKDIG
                     NILGTKDTGIVIYSDPVKMLEDSNPDLVVIATNSFVKVVKSQIIQAIKRHINVITIAE
                     EMAYPFVTNPEEAEEIDNMARRYGVTVLGTGINPGFVLDTQIITMTAAMLNVKKIKAA
                     RINDLSPFGPTVMETQGVGTSPEQFKEGLESGKIVGHIGFEQSIYMISQALGWEIDRI
                     EQTREPIISNVLRETKYVRVEPGMVAGCKHIAKAYCGDTLLIELEHPQQVRPELENVN
                     TGDYIWIDGDPSISVSTKPEIPGGKGTIALATNMIPFVIENIDPGLKSMIDMPMPRAL
                     LGDN"
     gene            14531..14854
                     /gene="ortA"
                     /locus_tag="PLU28_01795"
     CDS             14531..14854
                     /gene="ortA"
                     /locus_tag="PLU28_01795"
                     /EC_number="2.3.1.263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-oxopentanoate thiolase subunit OrtA"
                     /protein_id="HOG33830.1"
                     /translation="MQNEKQFFPKGSLVQICFTGLRPEERTANLPEDTRKVPLEIRVK
                     GFLKDESACIGQEVSVETVTGREVKGKMIAVNPKYEHNFGEPVPELILAVNKLQEIMK
                     GDFDD"
     gene            14847..16259
                     /gene="ortB"
                     /locus_tag="PLU28_01800"
     CDS             14847..16259
                     /gene="ortB"
                     /locus_tag="PLU28_01800"
                     /EC_number="2.3.1.263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-oxopentanoate thiolase subunit OrtB"
                     /protein_id="HOG33831.1"
                     /translation="MIDNSYEAVMSRKNEIMKKSVGVDYQKYEIHGIAFDYEKMLSDS
                     GYPIKKIRQIQLETGVGNTQLVELKNINRLIKKISPKGKGARIYVKDEATNPSGSFKD
                     RRASVSVYRAKELGFKGVIAATSGNYGAAVASQAAKKGLKSIIVQECYDSRGVGQPEI
                     LEKQRACEAYGAEVLQLSVGPELFYYFLRLLEDTGYFNASLYTPFGIAGVETLGYEIA
                     EQSIRQFGRYPDAVAITHAGGGNLTGTARGLKKAGAKDTKIIGVSVDLKGLHMASDRD
                     FNKKSFTTGHTGFGVPFAVFPDRADVPRNAARSLRYMDRYILVTQGEVFYVTEMLANM
                     EGLLRGPAGNTSLAGAIALATEMNEDQIIVVNETEYTGAGKLPSAQLTFARHNGIEIR
                     TGDPLKEDIPGKNIVIPSSPGLMGFTEYPLQSIRKSYLKQAVNKYGTKDFSDIELEFI
                     SEDINIGVEKVKEYLKEIAL"
     gene            16277..16648
                     /locus_tag="PLU28_01805"
     CDS             16277..16648
                     /locus_tag="PLU28_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine aminomutase subunit alpha"
                     /protein_id="HOG33832.1"
                     /translation="MIPRKDDFEVKAKRFEKMSDEELEKYFWNLTEKIVDPLIDLAYK
                     NTSPSIERSVLLRMGFSSLQAKALVDKIFEKGLLGKGAGNIVYRVSKDQNISMEQAGT
                     ELIQGRLWDVVQRIFEGVNKA"
     gene            16645..18837
                     /gene="oraE"
                     /locus_tag="PLU28_01810"
     CDS             16645..18837
                     /gene="oraE"
                     /locus_tag="PLU28_01810"
                     /EC_number="5.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ornithine 4,5-aminomutase subunit OraE"
                     /protein_id="HOG33833.1"
                     /translation="MKELNINEKINIDEILNDLENYAPRRKGWHWRKPLKEGTEINGF
                     KYFQISEPLKNSLPLPSAHYFGNIDPQPEVNITSEIASGRFEEDIRRMRMAAWYGADH
                     IMVIRTLGQSHMDGLIEGTPEGIGGIPITRKQIRASRKALDLIEDEVGRPINLHSYIS
                     GIAGPEVAVLFAEEGINGAHQDPQYNVLYRGINMVRSFVDAAVAKKIMASADMLQIDG
                     AHNANASAKKAWNVMPELLVQHAVNSLYSVKAGMKKGNIALSTVPPTTSPSPEMRINM
                     IYALTIRELFKGYRFRAQMNTRYIESDLMDATRIHVLNAALSRLTSADLQSTITPDEG
                     RNVPWHINSIRGIETAKHTLISMDGIKKYVKIDEANVREDIRNLKIRAILMLEEIIEM
                     GGFFNAVEEGMFVDNGFYPERMGDGIVRQKDGGIGAGTVVPRDQDYMAPVCEHFGYNN
                     LPAGIIKDCDPVGGCTLCHHNKIKFLNELDEDDNVQKRLLDIREYRKKDLIKPETEWS
                     YDGVIQIDMTIPESSEYAEAAAIEICRKMGLEDPEVISIKVLHPSEGTYVELKGKVPF
                     FINKKDLVLPKKTEALSEDVLFDFCKKRVIKVVAGTVGNDEHNVGLREILDIKHGGIE
                     KYGIKYVYLGTSIPPEKMIDSAIEVGADAVLASMIVTHNDIHIANMKKLNDIAVEKGV
                     RDKFLLIVGGTQISNDMAIENSMDAGFGRGTKGAHVASFIVKNMMKKA"
BASE COUNT         5915 a         3309 c         4059 g         5633 t
ORIGIN      
        1 agctatcaga gtatacggct agtttgtaaa ctgcggaaag tccgccgacc gaagttatat
       61 ttcccttatc tatgagaagc tcaggagaaa ctatactata cttaaaaagg gttttgcctt
      121 ctgtaaggac ataaaggaag tctccgttta ccttaaggtc gatgggacta cctatagcct
      181 tggaagcctt gatcatcata tctcctgaag tatcgactac taggagtttg ttagctgact
      241 tgcatgcaag ataaaggtaa tttcctttct tttccatgtc gtctatacct ggaaccttaa
      301 gcttcttaaa gtttgaaggg tcagttatat cagggaattt ataaatattt aaagaagtgc
      361 ctataaagag cttggttgcg ccttcagact ccattgtcag ggcacagaca gaccattctt
      421 ccttaagtcc cgtgaaggag gcagggatag tgtcaaacgc tccaagaaca tgactctgtc
      481 cgatgtagaa ctttggatct ttattatagt tgcctataac gcttatcatg tattgagggt
      541 ctgttttatt gttttgaaag tactctgatt ccactatagg gaataagcca ggagtgttaa
      601 aagcgcacga ggatgaaaca gtcccggagg atatgagagc agaatagtct acttcgtaaa
      661 gtccgttaaa gcgcgcagac acataagctt tcttaagcgg gaaagtactg ctgtccagag
      721 aaattccttc tttggttctg aaaaaatacg gcgaagcagc atttcccaga accgaaactc
      781 ccagagaact gtcttctgct cttatctgaa tgaagtaatc tttgttgtac agaagaggtc
      841 cttttatggt tgccgatgac tgagttgttt ctgaaaaagc actggatatt tccgaagatt
      901 caagatagtc tgatgaattg aaaagcttga ttgtgcaagt tacagtatct ccgtcagaat
      961 caggtgccga ccagtatata ggaatattgc ttatggccac atcggttaat ccggaaggaa
     1021 ttgccgtacc ttccttactg cccagcctgt ttactgatat tttaggaagt ccgtttgtgc
     1081 tgaacttcca tatctcactg tgaacctctc cggcagggta agctgcagaa acctgccagt
     1141 agtacgattt atcctcggac agtagaggca aggtagcagt ttttgataca agatcaatgt
     1201 ctgatgacgg gacttcattt gtcattgatg aaatgctgtt tccataatga attcttaaag
     1261 atgagggaga agcggtacca aaatactccc atttcaattt ggtatcaaga ggagttttta
     1321 cagccagatt ttcaggatat ggatatgttg gaaagcccat ttttttactg tagttggcgg
     1381 caaaaactat aagatcgtca accgtgattt tcatatcgcc tgtgcttatg tcataaaaac
     1441 catctttgtc gctatcaact gccgggtata tatcaactgc cgtattattg gcaaagtatg
     1501 cagcctgaaa aattgaaaga tctgttccgt ctatggatcc atctttatta aaatctccca
     1561 caaaggattg tatcatcacc acaggcgttc cttttgcagg aacaatcaca ggcaggtctt
     1621 taattgaact gtccagggta tcgattactt ttaaactgtc tacgtttata tcaagtgcag
     1681 ccgcaaggtt ctcaccgatt acactgaatc tgaaggttcc tatttttaaa ggtgaagtaa
     1741 agtccagtgc tttttccttt gaacgtgccg aacttgcaat tataaaaggc atagaagaag
     1801 catctgattt aagagatatg aaatctgtgt ttatcgaagg ggaaaatgtg aaatcattaa
     1861 gtgtgagagc ggtactggca aatgtgaatt ctaggccaaa agcatagagc ttttcttttc
     1921 ctttcctatt tacggcatat agatcgcaca aaagctcatc gcccttgaga gtaaagcttt
     1981 tcttgatata aagtccatat ttagagtagt ccgtatcgtt gcggtctata aagctacgta
     2041 cagtaagagc tttctctgaa agcgggagct cctcgtttat tatgtgtata tctttggatt
     2101 ttgctttttt tatggtcaga aaaggttctg aagattttat ggatccattt ttacctgcca
     2161 gtacaacttt gtactcggca ggatttatct gctgcatgat atggatagta tctcctgaaa
     2221 ataccagatc ttcacggtta gtgccttcag gagtttttat atgtaactgt ataccgtata
     2281 tatcacgcga tgttgaaaga actgctccat gatcgctgtc tgatatggaa atatttttgg
     2341 aatttatcgg ctgaaacgca gaacttttcg aagtgcacgc tgaaaataaa aaaactataa
     2401 taaacagaac agtaaccaaa tatgcatttt tcataaaaca cctctgacta tcttatatgt
     2461 attgatgaat tgataaccga ataacctgaa aaaggagtgt ttaagttttc ggaaaaaaaa
     2521 gtcgtatctg tttgcacaat gtgtatggct gtatcgccag gtgatttggc tttgaacttg
     2581 aaaaccaaca acaccatttc ctctgcgccg gctgaaaaag cttctgtcat agtcactccc
     2641 acaaccaaag agttgtaact gtgttttgtg aaaacaagag aagaatcagc cagaggactc
     2701 agagtataat agaacctttc gtcaacaaaa acaggagcca gatagttctt gtcaaaatca
     2761 ataaggatac tgaagctttt tatattagga aaatttttta tgaaaacagt ataacaaaac
     2821 tcttcgtcca caaagacgga gagttctttt gtatacactt ccggtttttc ctcatatact
     2881 ggtccgcaag ctgtaagtaa aagcatacag aaaaaaataa tcggaaggag ggattttttt
     2941 ttgaaaacga acatttttat atatctatca cataaagtca aggatggttt tggggactat
     3001 ctgtgccaaa gcttttaaag ctgcgtccat tactgatttt tgcatagcca tatcggtata
     3061 aacctttgct gggtcagcat ctgtctcagt agcaagaaac tcctccgaaa aagttttgaa
     3121 gttctccatc ctgctgttgg taagttcaag gagtctttga gttccgccta cctgagaaat
     3181 tgttctgctg gaggactcct gaatttcatc tatggctccg agttcatttt gaatgctttc
     3241 ggcagctttt ttgaggagtt cagatttgct ttcaattaat cctttcacag gagttgcggc
     3301 ggaaggatca ccctggagtt ctttgatttt ggtttcatta gttgctatcg agctgttttt
     3361 ttcatcgatc tgagtctgga gagaagcagc caaagcagta ttgccggcag cttcagcctg
     3421 gagctttctt ttttcaatct cttttatttc attttttaac gttacattct gcatgctcag
     3481 attatccttg gtttttatgg catcctttag gtctttggtt atctcgtcag atttaataaa
     3541 ggcatcttta agtctgtcta ctatcttaaa tatagaggtt ccgcttttgg ttccgaaagc
     3601 atcgtataca gttactccat actcaagctc gtatccgcca actgatattt tatgcttgat
     3661 atttgctttc ggatctgtca tgatgtctcc tgaatcacta acaggggttt tatcagactc
     3721 agcacctccg aaaataaact ttccagatac gcttgtattg gcaagagaaa tgagatgatc
     3781 gttaattttt tccagttcgt ctgcaatgct ttttctgtca aggttgctgt caagggttcc
     3841 agagttggct ccctgaacgg caagtgattt cactctggta tagactgacg atacatcctg
     3901 aagtatctga tcatacatct tggaaaagtt ttctacaata cttgcattgc tctgatagcg
     3961 ctctatttcc ctaagcctgc tatcgaggtt tgacattctt gaagcaatgg ctgcattgtc
     4021 cgaagggaaa ttaacttttt tgcctgaagt gagctgttca tttagcttac tgaactttcg
     4081 catggtgctc tgaagatcgc ttagtgccga attatttatc attatctctg taattctcat
     4141 tttattcctc ctatctgccc acaagcccca aaccgttgac tactttgttt atcatttcat
     4201 ctgcagcggt tatgactctt gcggaagcat tgaatgcttg ctgatacttt atcatattag
     4261 atacttcttc gtctattgag actcccttta ctcgttcgcg ctctccgtct atctgttctt
     4321 taagatactg gctgttggtt ttcatcttgc tggcattttc tccggagatc cccatctctg
     4381 ccacaagact ggcaagataa cctgagaagc ctacctttcc ttcactcagc agaggaagtt
     4441 cctttatctt tgagagatag ttccaaactg tggtgctgtg ttttcctata ggaatgaaat
     4501 aatcagcttt ccagtccccg ttggaatcaa tgcctttgcc aaggtctatt gaaagcgaag
     4561 atggattact tttcaaagta tctacaattt caagctgtct tacaagactt acatttttat
     4621 caagtttaag aacagatgag ttctgcaggt caacctcaag ttctctggcg gttctgtcag
     4681 gtcccataaa gacataattt gttttgtaag gaggttccga tgaattttta agtccaagaa
     4741 gctcaaaaaa cttttcatgt ccttttatgg tgatattacg catatcaaag tttacactgc
     4801 tgttggcttt taatgcgaat ctgccgtttg gtgtctgaaa agcactgaga cctgaaccgg
     4861 tggaatttat tttatctatc atgctttgaa aagtatctcg tgtaggatct atattgatgt
     4921 ttattccgtt tatctcaaga tttatcccgt attttataaa atctgatttc cacgttattt
     4981 caggatcgtt cataagtgcg ttgtaatcca ttccttctgc ggcaaaaaac tccttcgcag
     5041 actgcaagtc aaagagttta tcctttgaat aaagcgttgt gccgtttacc tttacctgaa
     5101 gatctatgat ctgttcaaag ttaaaatctg atttggttgg tattatttca attttatccc
     5161 cgttttttct gactttgaac tcaccggact tgcctatgga gtcttcggta aaaagtccca
     5221 gatccgtaag cggtattgaa gatacggttc cgtcagcgta ctccatgctt acttcaaagg
     5281 aggatgctgc atcaaaggat tttacatcgt aggttttggt tctaaaaacg ctcatgttga
     5341 aaacattctg cagggtaggt tctgatgtat ccaaaacgtt aagcctggtt tttgccgctc
     5401 ccatcttggt cattattccg tccttatcgt caaaaacgac attgtttatg ttgaaatctt
     5461 caacgttttt ggtaggaaca atgtaaagcg agtcatttaa tgtaaaggcc tttatcatcg
     5521 aagattgggc atttatatcc gccgcaactt tttcaatacc ctcgccggtg cctacagata
     5581 tatttacaac ttcctgtttc gaacttcctt tattaagaat aagctgtccg ctctctgcct
     5641 tgtttagttt tatggcttct atagctttgg ttttcagacc aagctgattg aatacgttgc
     5701 tgttaaaatc tattataagt ctgttgttaa gggaatctcc gccgtagttg ttcagagtgg
     5761 taagatcttt atttttatta tatgcaagtg acatttcaag actcttaagt ccatcggcta
     5821 tagttgaaat tttttctttg gttcccagag aaagtttttc tgaatcagta tttgtaaaat
     5881 cttccccaac tgatacaatc tgtccctttg ttatcatgtt tatttcatag ttctgaaagt
     5941 ctattccatt aaagcttctg cttccgggaa tatgattgac tgggccaccg ttaaaaaaac
     6001 atgatgaggc tattctgtaa agtctggcat tatcggaatc aagtttggca gacatatctt
     6061 taaaaaatct tgctccggta gtgtttcctg tagagtccca gccttcctgg tataccagat
     6121 taacagtatc gtttagagta agtgcaaact cgtccagctt acttctgtaa tcttttataa
     6181 cgctgtctct cagttccatt atagccttca tctttccatc gttgaaagat atttgggtgt
     6241 tattggaaaa cacagcatat ttatactgtt ctcccggaac tctttctact ttaagcggga
     6301 tgaatgtctt tccgttaagg acagttctgt caccaacgga aatagacatc tgaaagtctt
     6361 tatcatagtt tacccgtaca tttgaaagct ccgatatctg gtcaagaagt ctgtcacgct
     6421 catcaagaag atcgttgggt gaagaaccca tcacctggct ggaagaaatt ttatcgttca
     6481 gataggctat ctgcttgaaa gcagagttta actggtcaac tctggttttt atttcgtcat
     6541 taaggtctat ctgaagctgc tccagacgat agtcaatatc atagagaaca ttttttagct
     6601 ctgtggctct tgatacaatc tgacctttgg cagcttcgtt ggtaggatcg gtttttattt
     6661 cctccattga agaccagaac gaatcaaaaa atgttcgtaa accggtttca cccggttcac
     6721 cgaataactg ctctatataa tgcatatttc catttaaagt atcccaataa ttatatctgt
     6781 tagtgacctg tctgtactgt acatcaagaa actcatcccg tacacgctga atgtccgtta
     6841 cctttgagcc tgttccgaac tgcaaaggaa ttgaaggctg agtaaggctg tttactggta
     6901 tcggagatgt tgttacaatg agcggacgct gtcttgagaa tccctgagta ttggtgtttg
     6961 ctatattatg accgactaca ctcatagcta ttttatgtgt gtaaactcca aggagaccgg
     7021 tattgagtag tccgtataaa gatacgtcag gcataagttc atccctccca gttcagttta
     7081 ttttgatagt tgtttttgct gtaaccgctt ttagcataag tacttttgcc tgttttatct
     7141 atattaagca tatttagcaa tagattattg tagttgcttt caaaatcaag ggcctgatga
     7201 aggcaatcca atgtgaaagt cagattctgc atcttttcta cgaaaccggc aagttttaag
     7261 gcaacatcct tatctgtaac ggaaaaaaag tcgacaatat cttttatggc attaggaata
     7321 ttgtgacaag atgaaatatc cgctacaaac tggtctcttt ttatatctgc ctcctgaaac
     7381 tcctttgaga gcaagtcaat ttcagccaga agagagttta tgtactcggt agattctttt
     7441 tttattagtg attgcctgaa agaatccacc tttattataa gtcggtcaat caaaacaaac
     7501 tccttgtcaa gaatatcgac aaggccttta ttttcttcct tcatatctcg cccagcattt
     7561 tcttgactat ttctgatata ttaatactgt aagtgccttc gttgatagag ttcttatatg
     7621 actgaacgag atgattccta acgtttggaa tctttcttga aagttctata tacttcattg
     7681 aatctccggt aattttttca aactcttttg aaaaatcatt ttcagaagat gatttgatat
     7741 cttttaccgg atccttcttt atttgcttta actgagaaga gttgggtgat cccagacctc
     7801 ttatttccat agtagatacc tccgtttgga ttatttcaat aatttatcgt ctgttacgac
     7861 aaaaaattga gtatattctg ttataatttt cttcgaaagg ctaaagaggc ctatcagatt
     7921 catatgtttt tttaatccgg caattgtgat ataattgagt tcaaatgaac ataaatgatg
     7981 gaggtgggtt tagatgccag caaaaacagc ttatggaaag accgttcagg aaaaattaga
     8041 aaagacattt aaacacttgg aggaggagtt aaaggggttg cggacaggaa gaccttctac
     8101 taccatcttt gaaaatatca aagtagaatt ttacggtaac ccttcaccta taaatcaggt
     8161 gggatctctt aaccttaccg aggacaggac tctggtcatt accccatatg acaggtcgat
     8221 gctttccaag atagaaaaag ctatcaatgc ttccgatatc ggattgcatg ctataaatga
     8281 cggaatagtc attagagtct cctttcctac gccaacaggt gaagacagga aaaaatgggt
     8341 taaaaaagct aaagaacagg ttgaggaaac aaagatcgct cttagaaata taagaagaga
     8401 tgacctgaaa aaaataaaag atgatcagaa aaactccaaa ataacggaag atgagtccaa
     8461 aaaagccgaa gaagatataa ccaaggtact taaggaatac gaggataaat gtgatctttt
     8521 gtttactaaa aaagaaaagg agataatgga gtcttgacag gattaaaact gcctgaacat
     8581 gttgctataa taatggacgg caacggacgc tgggcaatag gcaaagggct cagcagaaac
     8641 ttaggacacg aaaagggtgc tcaagtagcg gaagaagtta ttcaatggtc ggcagatata
     8701 ggaataaaat atcttacact gtactcgttt tcttatgaga actggaaaag gccgaaagaa
     8761 gaggttaact atctttttga tctttttgta agatatcttg aaagcagaat gggtaagata
     8821 gtatcaaaag gtgtaaaaat gaggttttcc ggaagaataa atgaattacc tgactccctg
     8881 ataaaagcta tccggaatat tgaaaccaag agtgccggta acacaaagct tcagttaata
     8941 atggctctta actacggcgg taggcaagaa atagccgatg ctgttaataa agctttaaat
     9001 aacgggaaaa aagatatttc agaagaggat attgcaaaga atctttacct tgcggatgtt
     9061 cctgatccag atctgataat aagaacatca gcagaagaga gaataagcaa ctttcttctc
     9121 tggcagtctg catattctga actttacttc agcaaggttc tgtggcctga tttttcttac
     9181 gaagagtata tcagagcttt ggaaagttat tctaaaaggc agagaaggta tggcgggacg
     9241 gggagttgaa taatatgccg gttaacaaaa aagatttagc gttcaggact ctgtcaggtg
     9301 tgataatagg tccgatagtg gtcttttcat tcctgactta tcagagtttg ctcggtctgg
     9361 ttacgactat aataatttta gcttcttatg agtttattga gatgtccaat aaagaaataa
     9421 aaaataattt tataaagttg ctttatgctt ttgtaattgg tgtagcaagc cttctgtacg
     9481 gtttttcact gaagaccgaa tatgccggaa tactgccatt tgaagctgag ataatatttt
     9541 ttatagcttt tgtttttata gcttcggtaa gtctttttac tgcaaaggat cagaatgagg
     9601 ctaaaaatct tataacctcg tctgtatttg cgataatgta tatatctttt tttctttcta
     9661 ttttctataa catacatata ggttatggcg gaactatggg aattttagct ttaacctccg
     9721 tatgggccta tgatgcttgc gcgtacttct tcgggctcaa tttcggaaaa cacaaactgt
     9781 ctcctaaatt ttctccaaaa aaaacctggg aaggtttttt cggcggagta cttggaactt
     9841 ttgtatatat ccttatctat gagtttttca gagaacttta cggttttgcc aagcttgata
     9901 tattctacgc cttgatattt gcagtcatgg ttgcctgttt tgatacagtt ggggatctca
     9961 ccgaatcaat atttaaaaga ttttatggtg ttaaggattc aggcgaagtc cttccgggtc
    10021 acggagggat gcttgacaga atagatggtc ttctcgtagt tacacccatg tggtatctgt
    10081 ttttgaggat ccttggcatc tgaaaaacgg ggggtaggat gaaaaagttc acaaaatttc
    10141 gtgaaggttt tactgtgctt gaagttgttg ttgttcttgt tattattgca ataattacag
    10201 cagcggctat tcctgggttc agcaatatgg tctctcaggc taaagttaca aaggcgataa
    10261 acgatatgag gacggttcag acagctgtgg tgcagtatct tatttacaat agcgatactt
    10321 cggacataag tctggaaaag ctctatgatg gcggtttcat tggtagtaaa ccgttagata
    10381 tatcagtaag ttctgcttta agtgactctg aaatacatat tgtttacacc aaagccgatc
    10441 ctaagcatga taagttcatc aaagttgatc ccaacataga aaaagactct gatgaaaaac
    10501 cgatgcttag aataatgaaa aattagcttt tactattaaa acaagaaagg tatgcctggc
    10561 atacctttct tatgatataa ttgctggaaa agaaaggaac gatttttaaa ctgtcttgga
    10621 ggttataggt atgagctgga tgagtgctga tgaaatacgt gaaaggttct tagctttttt
    10681 tgaatccaaa ggacataaaa gacttgcaag ttctcctttg gttcctaatg atccacagct
    10741 tctttttacc gttgcaggta tggttccatt taaacctatt ttttggggaa aggttgagcc
    10801 gacgtataca agggttacca catgccaaaa atgtataaga accaacgata tagaaaatgt
    10861 tggaagaact ccgaggcatc atactttctt tgagatgctc ggaaattttt ctttcggaga
    10921 ttactttaaa aacgagtcta tagaatgggc atgggagttt ctgaccaaag aactgaagat
    10981 agatcctgac aggctgtggc cgtcggttta tctcgaagat gacgaagcat acagaatatg
    11041 gagagataag ataggagtaa aagaatcaaa aataatgagg tttgataaaa aagaaaattg
    11101 gtggggacct gtcggtccaa ccggtccatg cggtccctgt tctgaaattt atattgatac
    11161 aggcaataaa tcaaaatgtg aggatcctca gaattgcagt ccggcttgcg agtgcggaag
    11221 atttgttgaa atatggaata tagtttttac tgagtattac caggatgaaa acggaaaact
    11281 cgagcctctg gcaagaaaaa atatagatac cggtgcgggg cttgaaagaa tttcagcagc
    11341 tatgcaggga gtatacaaca actttgacag tgatcttttc cagccgacta taaaggtgat
    11401 agaaaaaagt ttcggtataa agtatggtga gtctgaaaaa gcagatgtgt ctataaaagt
    11461 aattgccgat cataccaggg ctgttacgtt tacagtagcc gaaggagttc ttccttcaaa
    11521 cgaggggaga ggctatgttc tgcgcagagt tctacgcagg gcattaaggc atggaaatct
    11581 tcttgggaaa aaagagcctt ttatgtccga gataatagac tccttagtag agaggatgga
    11641 aaaaatatat cctgagctct ctgaaaaaaa agaatttgta aaaaaaatag tcaaagcaga
    11701 agaagacaga tttctgcaga ctcttgaaaa aggtatagat aaactcaatg acattacaaa
    11761 tgccttatcc gataaaacca taccgggagc tgttgcattc gagctttatg atacgtatgg
    11821 ctttccgatt gatattacaa tagagattgc ccaggaaaaa ggttttgatg tagatgtgca
    11881 aagttttgag acatttatga aggaacagag agaaagagcc agaaatgcag ccggagaaaa
    11941 agaatacgac aaagctaacc aggcatataa aattgtcggt gaagagctta aaaatacagt
    12001 atttaccgga tatgaaaaaa atcatgatgt tcaaaaactt ttatatataa tcaaaggttc
    12061 tgaactttca gaaacagttt cagagggtga acaggctgaa ctgatatttg caagtacacc
    12121 cttctatgct gaaaaaggcg gacagatttc cgataccggt gagatacttt cagagagtgc
    12181 tgttgcactt gtagatgaag tcaaagtcat ttataatgaa gttgtatccc ataaagtttc
    12241 tgttaccaag ggtactataa aaaaagggga catcctttca cttcatatta atgaagaaaa
    12301 cagagctgct ataaagcgca atcataccgc cacacatctt ctgcacagtg ctttgagaat
    12361 agtagcaggt tcccatgtaa aacaggcagg gtcttttgtt gattctaaaa ggctacggtt
    12421 tgactttact cattttcagg ctctgagcca ggaacagata gctcagatag aaagaattgt
    12481 aaatactgaa attcttaaag cttatcctgt aattactgag ataaaatcac ttgaagaaac
    12541 aaaaaatgag aatgtaattg cccttttcca ggagaagtac ggcaataccg tacgggttgt
    12601 aaaagccgga ggattttcaa gtgagctttg cggcggaact catgtttcca atactggcga
    12661 gataggactt tttaaaataa tatccgaagt ttctgtctct tcgggaatac gaagaataga
    12721 agcatttact ggaataaact catatgagta cgttaacgag ctttcaaaaa aggttacgaa
    12781 cacatcaaaa attttggatg catcgtctga aaacattgaa gaaaaagctt ttcagacttt
    12841 agaacttaca agaaacctta ccaaggagat aaaaaggctg gaagaaaaac ttgccggaca
    12901 gtctctggac gggttcatta aaaatgcacg gatgataaat aacataaaag ttatctgcgg
    12961 tgtttttgaa aatgttgaac cagatgtgct cagaaacagt atcgatataa ttgcacagaa
    13021 agaaggagaa gctctgattg tacttttcag caggagcgat aaggtaatat tctgcgtgaa
    13081 agttccttct tttatgacta aagcacacaa tgccggtggt atagccaaaa aaatagctca
    13141 gtttcttggt ggaggcggag gaggaaaacc tgattttgca caggcaggag gaaaggatat
    13201 agcgaaagta gctcaggtta tttcggaaat agaaagattt atattctgag aagaatataa
    13261 actcatattc tattcatagt ataaagttca tattatgcca taatattaat aaaaaacacg
    13321 tatcaaaaag atcactcttt caagagtgat ttttttgtat gaatgcgttt tataaagttt
    13381 acaatgctta attcagcctt aatagcagat agtcttatat taacagcttt aatctgaatg
    13441 gatttttttt aatgaaaatt gtatcatata ttatactgaa tgaaaaaaat ttcatttaag
    13501 gaggggaata acatgtatag agttgcagta tggggtttcg gagctatggg aagcggtata
    13561 gccaggaaca taatgaccaa gtatgatcta agtttggtag gagtatatga ttccagggaa
    13621 gattttatgg gaaaagatat agggaatatt ctcggaacaa aagataccgg aatagtaata
    13681 tactcagatc cagttaaaat gcttgaagac agtaatcctg acctggttgt gattgccacc
    13741 aactctttcg tgaaggttgt taaatcgcag ataattcagg cgataaagag acatatcaat
    13801 gttataacga ttgcagaaga aatggcgtat ccttttgtaa ctaatcctga ggaagcagaa
    13861 gaaatagata atatggccag aagatatgga gtaacagtat tgggaaccgg tataaatcct
    13921 ggttttgttc ttgataccca gatcataact atgacagcgg ccatgctgaa cgttaagaag
    13981 ataaaagctg caagaataaa tgatctttcg cctttcggac ccacagtaat ggaaactcag
    14041 ggagtaggaa ccagtcccga gcagtttaaa gaaggccttg aaagcggtaa aatagtcgga
    14101 cacataggtt ttgaacagtc aatatatatg atttcccagg cacttggttg ggagatagac
    14161 agaatagaac agacaaggga accgataata tccaacgttt taagagaaac caagtatgta
    14221 agggttgaac ccggaatggt cgcaggatgc aagcatattg ccaaagccta ctgtggagat
    14281 acgctactta tagagcttga acatccccag caggtaaggc cggagcttga aaatgtaaat
    14341 accggagatt acatatggat agacggtgat ccgagcatct cagtttcaac caagccggag
    14401 atacctggtg gaaagggaac tatagctctt gctactaata tgataccttt tgtaatagaa
    14461 aacatcgacc caggacttaa gagcatgata gacatgccaa tgcccagagc tttactgggg
    14521 gataattaac atgcagaatg aaaaacaatt ctttccgaaa gggagtcttg tacagatatg
    14581 ttttacaggg ctcaggcctg aggagcgtac tgctaatctt ccggaggata ccagaaaggt
    14641 tccgcttgaa atacgtgtga aaggtttttt aaaggatgaa tctgcatgta taggccagga
    14701 agtatctgta gaaacagtga caggaagaga ggtaaagggt aagatgatag cagtcaatcc
    14761 caaatatgaa cataattttg gggagcctgt ccctgaacta atacttgccg taaataagct
    14821 tcaggagata atgaaaggtg attttgatga ttgataactc atatgaagct gtaatgtcca
    14881 gaaaaaatga aataatgaaa aaatctgtgg gagtcgatta tcagaaatat gaaatccacg
    14941 ggatagcttt tgattatgaa aaaatgcttt cagacagcgg atatcccatt aaaaagataa
    15001 ggcaaataca gcttgagaca ggagtaggga atactcagct tgtagaactt aaaaatataa
    15061 acagactgat taaaaaaata tctcctaaag gaaaaggggc gcgaatctac gtaaaggatg
    15121 aagcaaccaa tccatcggga tcttttaaag atagaagagc atctgtaagt gtatatagag
    15181 ccaaggaact tggctttaaa ggagtaatag ctgccacaag cggcaactat ggtgcagcgg
    15241 tggcttccca agccgcaaaa aaagggctta agtctattat agtgcaggaa tgctatgatt
    15301 caagaggagt aggacagcct gaaattttag aaaagcaaag ggcatgtgag gcatatggag
    15361 cggaagtact tcagctttcg gttggaccag aactttttta ttattttctt cgtcttctgg
    15421 aagatacagg ttactttaac gcttcacttt acacaccttt tggaattgcc ggtgtggaaa
    15481 ctctcggtta tgagatagct gagcagtcta tacggcagtt tggcagatat cccgatgctg
    15541 tggcgataac tcatgcaggc ggtgggaatc ttaccggaac agccagggga cttaaaaaag
    15601 caggagctaa ggatacaaag ataataggtg taagtgttga tcttaagggt cttcatatgg
    15661 ccagtgacag agattttaac aaaaaatcct ttaccacggg acatacaggg tttggtgtac
    15721 cttttgcagt atttcccgac agggcggacg tgccaaggaa tgcggcaaga tctctaagat
    15781 atatggacag gtatattttg gttactcagg gagaagtttt ttatgtaaca gaaatgcttg
    15841 caaatatgga aggtctttta agaggaccgg ccggcaacac ttcccttgcc ggggcaattg
    15901 ctcttgcgac agagatgaac gaggaccaga taattgttgt aaacgagaca gagtataccg
    15961 gagctggcaa gcttccgtct gcacagctta catttgcaag gcataacggt attgaaataa
    16021 gaacaggtga tccgttaaaa gaagatattc ccggtaaaaa cattgtaatt ccgtcatcgc
    16081 ctggtcttat gggatttaca gagtatcctc tgcaatctat aaggaaatct tatctgaagc
    16141 aggctgtgaa caaatacggg acaaaagact tttctgatat agagcttgaa tttatatccg
    16201 aggatataaa tatcggggtt gaaaaagtta aagaatactt aaaagagata gctctttgag
    16261 atttcggggg gaaaaaatga ttcctaggaa agatgatttt gaagtaaaag ctaagagatt
    16321 tgaaaaaatg agtgatgagg aactggaaaa atacttttgg aatcttaccg aaaagatagt
    16381 tgatcctctc atagatcttg catataagaa cacatcccct tcaatagaaa gatctgttct
    16441 tttacgaatg gggttcagtt ctcttcaggc taaagcactt gttgacaaga tatttgaaaa
    16501 aggtcttttg ggcaaaggcg caggaaatat agtatataga gtctcaaagg atcagaatat
    16561 cagcatggag caggccggaa ccgaacttat acagggaagg ctctgggatg ttgtgcagag
    16621 gatttttgag ggggtaaata aagcatgaaa gaattgaata taaatgaaaa gataaatatc
    16681 gacgagatac tcaatgatct tgaaaattat gctcccagaa ggaaaggctg gcactggcgc
    16741 aagcctctta aagagggaac agaaataaat ggttttaagt atttccagat aagtgaacct
    16801 cttaaaaact cactgccact tccttctgct cattactttg gcaacattga tccacagcca
    16861 gaagttaaca taacttctga aattgcttct ggaaggtttg aagaagatat aagaagaatg
    16921 agaatggcag catggtatgg tgcagaccat ataatggtta tcaggactct cgggcagagt
    16981 catatggacg gactgattga aggtactcct gaaggaatag gcggcattcc tataactcgc
    17041 aaacagatca gagcttcaag aaaagctttg gaccttatag aagatgaagt aggtaggcct
    17101 ataaatcttc acagctatat aagcggaata gcaggaccgg aagttgcagt tctttttgcc
    17161 gaagaaggta taaacggtgc acatcaggat ccgcagtata atgttcttta caggggaata
    17221 aatatggttc gctcctttgt ggatgcagcc gtagcaaaga aaataatggc aagcgcagat
    17281 atgctccaga tagacggggc ccacaatgcc aatgcatcag caaaaaaagc atggaatgtt
    17341 atgcctgagc ttctggtaca gcatgcggta aactccttgt actctgtaaa ggcaggaatg
    17401 aaaaagggta acattgctct ttcaaccgtg cctcctacga catcgccttc accggaaatg
    17461 agaataaata tgatctatgc gcttacaata cgtgaacttt ttaagggcta tagattcagg
    17521 gcacagatga acacacggta tatagaatca gatcttatgg atgcgacaag gatacacgta
    17581 cttaatgcag cgctttcaag gcttacaagt gccgatctgc agtctactat aactccagat
    17641 gaaggcagga atgttccatg gcatatcaat tcgataagag gtatagagac ggccaagcat
    17701 actctgatat ctatggacgg cataaagaag tatgtaaaga tagatgaagc aaacgtccgt
    17761 gaagatatac gcaacctcaa aataagagct atactgatgc ttgaagaaat catcgaaatg
    17821 ggtggatttt tcaatgctgt tgaagaggga atgttcgtag acaatggttt ttatcccgaa
    17881 agaatgggag atggaatagt aaggcagaaa gacgggggaa taggcgccgg aacagttgtt
    17941 cccagagatc aagattatat ggcgccagta tgtgaacatt tcggttacaa taatctccct
    18001 gcagggatca taaaagactg tgatccggta ggtggttgta ccctttgtca ccataacaaa
    18061 attaagtttc tcaatgaact ggatgaagat gacaatgtac agaaacgatt gctggatata
    18121 cgtgagtaca gaaaaaaaga tcttatcaag cctgaaacgg aatggagtta tgacggagtg
    18181 atacagatag acatgaccat accggaaagt tccgaatatg cagaagctgc agcaattgaa
    18241 atatgccgta aaatgggtct tgaagatccc gaagttatta gtataaaggt tctacatcca
    18301 agcgaaggaa catatgttga gcttaaaggt aaagtgcctt ttttcattaa caagaaggat
    18361 cttgtacttc ccaaaaaaac tgaagcactt tctgaagatg tactttttga cttctgcaaa
    18421 aaaagagtaa taaaagttgt agctggtact gtgggaaacg acgagcataa cgtcggactg
    18481 agggaaattc ttgatataaa gcatggcgga atagaaaagt acggtataaa gtatgtttat
    18541 cttggaacca gtattcctcc tgaaaaaatg atagattctg caatagaagt tggtgccgat
    18601 gccgtgcttg cttccatgat agtcactcat aatgatatac atattgcaaa catgaaaaaa
    18661 cttaacgata ttgctgtgga aaaaggggta agagataagt ttcttcttat cgtaggcgga
    18721 acacaaatct ctaatgatat ggctatagag aacagtatgg atgcaggttt tggtagagga
    18781 accaagggag cacatgttgc tagttttatt gtaaaaaata tgatgaaaaa agcttaggaa
    18841 taaaaaaacc gggcctttat gaaaatgaag tgaacccctt tagttagaca aaatctgacg
    18901 aaaggggttt tgttat
//
LOCUS       DAOSHR010000018        18418 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_2464_length_18418_cov_5.822306, whole genome shotgun sequence.
ACCESSION   DAOSHR010000018 DAOSHR010000000
VERSION     DAOSHR010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 18418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18418
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2464_length_18418_cov_5.822306"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /locus_tag="PLU28_01815"
     CDS             complement(<1..624)
                     /locus_tag="PLU28_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOG33834.1"
                     /translation="MPNVIINDIKVEVPENVTVLEAIRLSGGYVPTLCHMKLDDIKIE
                     NNPASCRICMVEVAGRRNLAPACVTPIAEGMVIKTHSKKAVNARRTVLQLLLSNHPQD
                     CLKCAKNGQCELQDLASELFITTNPYMGNKSTHKKDVSAAIIRDPSKCIMCRKCETMC
                     NSFQTVGVLSAVDRAFDAVVKPTFDLPLEQTNCTFCGQCVAVCPTGAL"
     gene            complement(642..2435)
                     /gene="nuoF"
                     /locus_tag="PLU28_01820"
     CDS             complement(642..2435)
                     /gene="nuoF"
                     /locus_tag="PLU28_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HOG33835.1"
                     /translation="MGKYKQFILICGGTGCSSSGSGDIIDSFNKALEEKGLKDEVQIV
                     RTGCFGFCEQGPIVKVLPDDTFYVKVTEKDVENIVNEHIIKGRRVQRLLYVEPSEKEK
                     IEEYIKMPFYKKQIRVVLRNAGAINPENVEEYIANDGYSALAKALTEMSQDDVLKSIS
                     DSGLRGRGGGGFPTGMKWSFAKRAKGDKKYIMCNADEGDPGAFMDRSVMEGDPHSVIE
                     GMSIAAYAIGAQKGYVYIRAEYPLAVQRLRKALNDAREYGFLGENVMGTNFSFDIEVR
                     LGAGAFVCGEETALIHSIEGKRGEPTSKPPFPANSGLWKKPTVINNVETLAQIPVILL
                     KGADWFRKLGTEKSPGTKVFALAGAINNVGLVEVPMGITLREILYEIGGGIRNNKQFK
                     AVQTGGPSGGCIPAKYLDTPIDFDNLIALGSMMGSGGMIVMDEDTCMVDVSKFYLEFT
                     VDESCGKCTPCRIGNYRLHEVLERITSGRAVIEDLDKLEQLGNVIKDTALCGLGQTSP
                     NPILSTINYFRDEYVAHIQDKKCPAGVCKNLIRYDINPAKCVGCTACARVCPVQAITG
                     TVKKAHVIDQDKCIKCGACYATCRFGAISKI"
     gene            complement(2453..2848)
                     /locus_tag="PLU28_01825"
     CDS             complement(2453..2848)
                     /locus_tag="PLU28_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOG33836.1"
                     /translation="MPIVKSVADLMKIKEQAKEKIQVRKTADSESKVVLKVAMGTCGI
                     ASGAKETFSALIEEIDKRKLNNIIVVQTGCMGYCHSEPTIEVIEPGKDSIIYGKMTKD
                     RAGELIQKHIIDGELLQDSIIGKTHQVAK"
     gene            complement(2872..3387)
                     /locus_tag="PLU28_01830"
     CDS             complement(2872..3387)
                     /locus_tag="PLU28_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOG33837.1"
                     /translation="MNETMEMKLRDVADYVDTLKLEGKDQEVKRSYLIHTLHKAQEII
                     GYLPVEVQKLIAKKLGLHASEVYGVVSFYNYFTMKPKGLYPINLCLGTACYVRGSGPL
                     YEELKKLLGVEEGEVTSDGIFSLHAVRCLGACGLAPVLMIGEKVHGRLKKEDLPRILE
                     EYRQLAKTQNV"
     gene            complement(3540..3806)
                     /locus_tag="PLU28_01835"
     CDS             complement(3540..3806)
                     /locus_tag="PLU28_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG33838.1"
                     /translation="MTDKIFEINSKDISKLSFKDLIFYIEEINNFFKNQPKDETVDID
                     LAIDLYKKALELLMQAKSKLRIAREEKEKIDKVYAGLLEKNEND"
     gene            complement(3803..5188)
                     /gene="xseA"
                     /locus_tag="PLU28_01840"
     CDS             complement(3803..5188)
                     /gene="xseA"
                     /locus_tag="PLU28_01840"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOG33839.1"
                     /translation="MDFPSLKDLADYINGIFTSSDLYNQSVKIIGDVSSAKYSSKNDL
                     YIELSQQTGASNYSMTVFFPNSYASYVLERLDIKNPNELVGKKWEFMGNITLWKGNLR
                     FVMTGKALFPVGDSNIELKKMKILSLLKEKGFLRIKERTLNELEPIKKIAVVSSPTAA
                     GYQDFEKNIIAAKNIPVVHLYPALMQGAETVDSVINAMTNIIKSKIPYDVVALIRGGG
                     SKSDLMYFDDVKLGVYIAKFNEIIPVLTGIGHEQDTSIPDYVSFKRYSTPTEAARDIV
                     RQIDEVDEIFVGKIDDLKESFDFYQKTIEDLISLKNTDLMRITLENCIFYQNKSFDDF
                     HKIIDNSIENIFSLYEKNFSYTILSTFSESISSELKYKNALIESQKSEIGMFFNDQIS
                     FFENYLNEQYAEITGQSPFGAFMNNGAVIRKNSYLISRVKDLNLDDEVEMYFPDGKAC
                     AKIIRKEDQAN"
     gene            complement(5190..5888)
                     /gene="rsmI"
                     /locus_tag="PLU28_01845"
     CDS             complement(5190..5888)
                     /gene="rsmI"
                     /locus_tag="PLU28_01845"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOG33840.1"
                     /translation="MAKLIIVATPIGNLEDISPRALQALKECDMIFTEDKRVIIKLLN
                     HYAIGEKKLFTFCEAKAERTLEPAISELKKCITAVFVSDAGMPVVSDPGYQLVERCYE
                     LGIQIDVIPGASAPVTAIAASGFPGSKFYFLGFIPRDKNQRRMWKTLVDFPNIMVFFE
                     SPNRIVQTLENILQILGDREVFFAREMTKIHQEFFRTTVSQAIKEFSQRKEVKGEFTV
                     VLSGKMHIRQTEEE"
     gene            complement(5881..6435)
                     /locus_tag="PLU28_01850"
     CDS             complement(5881..6435)
                     /locus_tag="PLU28_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG33841.1"
                     /translation="MKIEKERKYIFDDQTVALNLIRKAKTRSLIFQWYLDEGERIRLE
                     ISSEKFSWTRNFKTEIDIQSRYEDESSLNPSDVEFNRLSTYPVVVKNRYFLEYDPEII
                     VDEILNPAEMIKYNMPQTGEKLFLMEIEEKNTFVEDLSEFVNKIFTNESLIDVTGLKK
                     YKNSEIAGLCKEHFLKLFYEVIRG"
     gene            complement(6525..6611)
                     /locus_tag="PLU28_01855"
     tRNA            complement(6525..6611)
                     /locus_tag="PLU28_01855"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6575..6577),aa:Leu,seq:gag)
     gene            complement(6687..7241)
                     /gene="nadD"
                     /locus_tag="PLU28_01860"
     CDS             complement(6687..7241)
                     /gene="nadD"
                     /locus_tag="PLU28_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HOG33842.1"
                     /translation="MTVVFGGAFNPPHVGHRIVAEAAYDLLKPEKFLIIPTFISPHKM
                     GSDIAAYDKRMEWCRKTFPCERFYVSDIESTLDGRSYTYNTVTKLLLSYSDVYLLIGE
                     DSFLSFNRWYMAEELYKLCKLVVYPRFFDERGFLGSTRQFIKLQSPIIDISSSIIRKS
                     LSLGLNVYGMVDDAIYNEIKVNYK"
     gene            complement(7252..8577)
                     /gene="obgE"
                     /locus_tag="PLU28_01865"
     CDS             complement(7252..8577)
                     /gene="obgE"
                     /locus_tag="PLU28_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOG33843.1"
                     /translation="MEFDFLDEAIIEITAGKGGDGAISFRREKFAPFGGPDGGNGGTG
                     GHIIIQSVLEKNTLVDFKYKKKFLAENGENGKTKNQYGCDGEDMVIEVPVGTVIYNYP
                     DMDKIADLQYPNEYVIVARGGIGGRGNSKFATSTLQAPRISEKGIEGEKKVLKLELKL
                     LADIGIVGFPNVGKSSLISKISNARPKIANYPFTTLIPNLGVVKVDEGKSFVVADIPG
                     LIEGAHEGIGLGDKFLKHIERCFALVHIIDGSEYEGRDPVNDYYSIRKELEKFNKDIS
                     LKEEVIVLNKTDTLSEDKLVTICSKIEKAIGKPVIPISCYTGYNVDRLKGIMYSIIED
                     RREEEIRKYEKLKRSMPEKIKFDAEPVKVETVMKIKYQVVRIAEHEYEIVGEDIVQLL
                     SKFNIFQMDSRKKILGILEKNGLEKLLRRAGVSEGDTIYCGEFAFEYIR"
     gene            complement(8578..9558)
                     /locus_tag="PLU28_01870"
     CDS             complement(8578..9558)
                     /locus_tag="PLU28_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOG33844.1"
                     /translation="MTDNKSITIKQRESMARKGSLIGIAGNAVLGFLKLSIGFFTGSL
                     AITADGIDTVTDIFTSFLTLIATKLSSKPADESHPYGHEKIEPIITKILSLIVIYAGY
                     EVLSSAISRLGGKQSEIKDVSLILIVSAISVFTKFFLYRYKLAIGKKINSSSFVADAL
                     NMRNDVFTSISVLAGVFLIFITGIWWIDALLAIFVSVFIFKVGISLFMESSNELMDGS
                     KELGDVYNDILKGMESFHQVKNPHRIRVRKSGYIYFVEMHIEIDPQMTVHQAHMIVKN
                     IENEIKKENPYIRDVIIHIEPIDNEEKEDFGFNVRSIKKTFGKKGNNGGK"
     gene            9833..10168
                     /locus_tag="PLU28_01875"
     CDS             9833..10168
                     /locus_tag="PLU28_01875"
                     /inference="COORDINATES: protein motif:HMM:NF015958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF370 domain-containing protein"
                     /protein_id="HOG33845.1"
                     /translation="MFVSITKDSFIAAERVHSIIPQDFIQFRRMKKMFQGDDVLSEGE
                     RRGRADASSQEEKLDEEFIKGSTSLIDITYGKSVETIIFMDSGHLILTCVEAKKIIDK
                     VKKSRRGES"
     gene            10171..10773
                     /gene="clpP"
                     /locus_tag="PLU28_01880"
     CDS             10171..10773
                     /gene="clpP"
                     /locus_tag="PLU28_01880"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HOG33846.1"
                     /translation="MPMPVVIESDGRYERSYDIYSRLLKDRIIFLGTPIDDTVANVII
                     AQMLFLESQDPDKDISLYINSPGGVITAGLAIYDTMHYIKPSVSTICLGQAASMAAVL
                     LAAGAKGKRLSVPNSRIMIHQPSGGMEGTAADIEIQTREILRMRSQLNQILSSHTGQP
                     LKRIEKDIERDYFMSAQEALDYGLIDRVFETKKTDDKNNK"
     gene            10830..13286
                     /gene="mutS"
                     /locus_tag="PLU28_01885"
     CDS             10830..13286
                     /gene="mutS"
                     /locus_tag="PLU28_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOG33847.1"
                     /translation="MKDQEFTPMIRQYMEIKKDYSDSILLFRLGDFYETFFDDAKTMS
                     DTLQLTLTKRAGHPMAGIPYHALDNYLKRLIDNGFKVAICDQMEDPALAKGIVKRSVT
                     RILTPGTVVEDSMLDEGNRFSILIDKKNNFTFSIFDFTTGEVYLDSMDSTDEEMIDFM
                     LSYGIVQILLSSNLKYMLKIIKERRPGIYCEVVEDWILSGNYDSHLKSVYSVSSLDFL
                     EFSSCELVLLDGVFKYLETTQFKTIKHFHFPKKFRSSDYMFLDSNTIYNLGVVPQPEQ
                     RGKSLFDTLNYCKTNMGKRFLAENLKRPLTDSTHIEKRLNAVQMLTSDKKLLTEIQDS
                     LCGIMDLERISSRISIQKSYPKDINALSSSIEKSSELSYIIMQNIGLYEIFGAIPKLM
                     DVYEKIGRYIFEDASNDVGSGMVIKDGISSELDEYRSISRDIDSVLRELEKREKETTG
                     LSSLKIGRNRIYGFYIEISKVQAQSAPLSYTRKQTLTNCERFVTPELNELEKKVLVAQ
                     EKIRKIETSIYDEFIVSLSSYLKRIKELSAVISEIDLACSLSQAAIRNSYIRPFFVEK
                     QEEIEIIESRHPMVEKTVDNFQKNDFAISGGKNFIIITGPNMSGKSTYLRQIGLISIM
                     AQIGSFVPAQKAFITIYDRVFTRIGAKDDVVSGKSTFLVEMLEMSGILSNSSQKSLIL
                     LDEVGRGTGTLDGIAVAWAISEYLFQVIGAVTLFATHYTELTIMSEIYECVANKRVRI
                     LESENGIVFLHKIEEGSSSSSYGIEVAKLAGFPAEITQRALEILEQLSDKADLENRLK
                     RIKNIKRRKYRLDENQLKMF"
     gene            13335..14435
                     /locus_tag="PLU28_01890"
     CDS             13335..14435
                     /locus_tag="PLU28_01890"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HOG33848.1"
                     /translation="MEDTLLKKKLEDIRLYMRSNFLDCFIVINYESSSRSTSRFLTGF
                     QGTFCIIFISQEDFDFYTDSRYLEYVEYGYKSLNVKIYDGNLIQLLKKLLSAFKPGAK
                     IGYEASNISVRFYKRFFEVFKNFSFVGCEDIFLSLRTKKNASEIDNIKKAVIISEKSY
                     LESIERFEYGMSEKDFSALLEYRMRINGADSCAFETIVASGKRSCMPHGYASDKKIEK
                     GDIVIVDFGACLNGLNSDITRVFCVNKIDPRYKKAYDVILSAQNLAIEASRPGMLSSD
                     LDGVARGIIKKYGLSSYFTHTLGHGVGYDVHEEPKISKNSSYILESGNVFTIEPGVYF
                     PGDFGVRVEDTILLTEKGHQNFCSLTKEIIKL"
     gene            14488..14901
                     /locus_tag="PLU28_01895"
     CDS             14488..14901
                     /locus_tag="PLU28_01895"
                     /inference="COORDINATES: protein motif:HMM:NF015721.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HOG33849.1"
                     /translation="MPVNQHNTFGEISISEAVLKDITLKTVETFLKAEKIYTDKVKRD
                     LPKNVRITADENGSVSIGLKITGKYGENLIDFTKKLQILVKEEVEKMSEINVATIDIT
                     IEELEYPESDDTEEHELEEAQEEHNGDNGEEQANK"
     gene            14926..15366
                     /gene="nusB"
                     /locus_tag="PLU28_01900"
     CDS             14926..15366
                     /gene="nusB"
                     /locus_tag="PLU28_01900"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOG33850.1"
                     /translation="METPLRILRECVVESTFQINFSDVSQDMLIFSLEDSMNFKKLPK
                     KYYSVAYEYVVGINQNRQLIDAIIEKHLENWTFSRLGNIEKCILRMSVFEILKKPEIP
                     LKVIFDESVEISKKYCDDESSKFVNGILDRISRDSDIISHKASD"
     gene            15368..17203
                     /gene="dxs"
                     /locus_tag="PLU28_01905"
     CDS             15368..17203
                     /gene="dxs"
                     /locus_tag="PLU28_01905"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HOG33851.1"
                     /translation="MQEQPLYKLLRQMDYDQLDELAQEIRQYIKKVVFSNNGHLASNF
                     GVVELTLSLYRVFDPSQDVVIWDTSHQSYVHKLLTGRWNSFSTLRSLNGISGFTSIKE
                     SFYDRFGTGHAGTSISAALGYSLADSYKNNNRSVIAVIGDGSINCGMALESLNQLKYQ
                     NANIKIILNNNDMSISNNVGALAGIFSRLRAKREYYQFKQFVKDSLSEAPFGQDLEIV
                     LKKIKDSIKYSVYRSPVGYFEDMGIKYFGPVDGHNISELDVALSLLKEYNDSPAILHV
                     ITKKGKGLEEAEKNPTKFHGVSKPSENKNPSYSTIVGKVLAHLKGYDFISFTAAMSDG
                     TGLEELKKVAPGKVLDMGITEPSIVTAAAAFSLNGILPVVAIYSTFMQRAFDSIVHDV
                     ALQNAPVLFLLDRAGLVGEDGPTHHGVFDISFLRLIPDIRILTPLDGQDLADMIYTAV
                     VNGIKEPTFIRYPKEYENNTIQNILDSLKTVNEKWIYIKKSTSKNYILGVGTICKTLK
                     ECVKDYDINVIGVRSIKPLDTAVMQELTQKAENILVFEEGSLKGGFNEEIFKLYGKNV
                     LPFGIKDSFIPAGSRQELLNMCGLDHQKIKEAIDAFIRSKERDMA"
     gene            17200..17766
                     /locus_tag="PLU28_01910"
     CDS             17200..17766
                     /locus_tag="PLU28_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG33852.1"
                     /translation="MKKLIVMSLLSFIIFISAFSFDILVNTSYSFTLDATVQTSSGIP
                     SAYDRSQKLNYTGIRISYLIPTEDSKEILYGPYIGFFYNLFDKQTGSYPQLNADVKDI
                     SYSMGLNVVYTSKLFQSINFDVCAYGGVHTDDNFKTYSTEILMSGGFNWDFLFINAGY
                     DIRYYYVNKNNTIAVNSIPITVGVSFKF"
     gene            17763..>18418
                     /locus_tag="PLU28_01915"
     CDS             17763..>18418
                     /locus_tag="PLU28_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG33853.1"
                     /translation="MSNFRADLEIEKIFELISNFCVTPYGKDYFYKNIVPIKDYNILK
                     RETILSQDVFDIYKSGREFELYGLADIREILGKLKQGSFLEPIEFRKIANFLNHLNAA
                     FPKNEEFYTERLLMILQSIVLHKEYVDKVYKTVSQEGEIMDSASAELSRIRKDLNKTR
                     VLVNSNINALAQKYAKYLAIDKPVIRNSRLCLVVRGEFRGNVPGMLVGKSDSGFSLYI
                     "
BASE COUNT         5774 a         3506 c         3175 g         5963 t
ORIGIN      
        1 aagggctcct gtgggacata cagccacaca ctgcccgcag aatgtacagt tggtctgttc
       61 aagcgggaga tcaaacgttg gctttacaac agcatcaaaa gctctgtcaa ctgcggaaag
      121 cacaccaacc gtctggaagg aattacacat ggtctcgcac tttctgcaca ttatacattt
      181 gctgggatct cttattatgg ctgcagatac atccttttta tgagtagatt tatttcccat
      241 ataaggattg gttgtaataa ataattctga tgcaagatcc tgaagttcgc actggccgtt
      301 ctttgcacac ttcaaacaat cctgaggatg attggatagg agaagttgaa gaacagttct
      361 tcttgcattt actgcctttt ttgaatgagt tttaataacc attccttctg caataggagt
      421 aacacatgca ggagcaaggt ttcttcttcc ggcaacttca accatacata ttctgcatga
      481 tgcaggatta ttctctattt ttatgtcatc cagcttcata tggcaaagag taggaacata
      541 tcctccggaa agccttatag cttcaagtac agttacattt tccggtactt caacttttat
      601 atcatttatt attacatttg gcattatgaa agtccctcct tttatatttt acttatagcg
      661 ccaaatctac atgtcgcata acaggcgccg cattttatac acttatcctg gtcaataaca
      721 tgagcttttt tgacggtacc ggttatagcc tgaaccggac atactctggc acatgctgta
      781 catccaacac attttgccgg atttatatcg tatcttatga ggtttttaca tactccggcc
      841 ggacattttt tatcctgaat gtgggctaca tactcatctc tgaaataatt gattgttgaa
      901 aggataggat ttggagaagt ttgacctagt ccacataatg ccgtatcttt aataacattg
      961 cccaactgtt ccagtttatc aagatcctct atgacagccc tcccggatgt tattctctcc
     1021 agtacctcat gtagtctgta atttcctatt ctgcatggtg tacactttcc gcatgactca
     1081 tcaacggtaa attcaagata gaacttggaa acatcgacca tacaagtatc ttcgtccata
     1141 acaatcatac cgcctgaacc catcatagaa cctaaagcaa taagattatc aaagtctatt
     1201 ggtgtatcaa ggtactttgc aggaatacat ccacccgacg gtccgcctgt ttggacagct
     1261 ttgaactgct tgttgtttct tattcctccg cctatctcat aaagaatttc tcttaaggtt
     1321 attcccatag gtacttcaac aagtccgaca ttgtttatgg ctccggccaa tgcaaagact
     1381 tttgttccag gggacttctc tgtaccaagc tttctgaacc aatctgctcc tttaagcaat
     1441 ataacaggaa tctgcgctaa ggtctcaacg ttattgatta cagttggctt tttccacaag
     1501 ccgctgtttg ctgggaaagg aggcttggat gtaggttctc ctctcttacc ctctattgaa
     1561 tggataaggg ctgtttcctc accacagaca aatgctccgg caccgagtct tacttctatg
     1621 tcaaaagaga agtttgttcc cataacgttt tcaccgagga aaccatactc tcttgcatca
     1681 ttcaatgctt ttcttaatct ctgaactgct aacgggtact cagctcttat ataaacatat
     1741 cctttctgtg cacctattgc ataagcagct atagacatac cttcaattac cgaatgggga
     1801 tctccttcca taacggacct gtccatgaat gctcccggat ctccttcatc ggcattacac
     1861 ataatgtact ttttatcacc cttggctctc ttggcaaaac tccacttcat accggtagga
     1921 aaacctccac cgccccttcc cctcagtcct gaatcagaaa tactcttaag gacatcgtcc
     1981 tgggacattt cggtgagtgc tttcgcaagt gctgaatatc cgtcgttagc aatatactct
     2041 tcaacgtttt caggattaat cgctccggca tttctgagaa ccactctgat ctgtttttta
     2101 tagaaaggca tctttatata ctcttctatc ttctcttttt ctgacggttc aacataaaga
     2161 agtctctgaa ctcttcttcc ttttattatg tgttcgttta caatattttc tacatctttt
     2221 tctgttactt taacgtaaaa cgtatcgtct ggaagaactt tcactatagg tccctgttca
     2281 cagaatccga aacatccggt tctgactatc tgaacttcat ctttaagtcc tttttcttcc
     2341 agtgctttat tgaaagaatc tattatatct ccgcttcctg aagaggaaca tccggttccg
     2401 ccgcagatca ggataaactg cttatatttt cccattattt taacctccaa tcttattttg
     2461 caacttggtg tgttttacca attattgagt cctgtaataa ttcgccatca attatgtgct
     2521 tctgaataag ttcaccggct ctgtctttag tcattttacc gtatattatg gagtcttttc
     2581 caggttcgat aacttcaatt gtaggttcgg aatggcagta tcccatgcag cctgtctgaa
     2641 ctacgataat attgttcagt tttcttttgt caatttcctc tataagggct gaaaaagttt
     2701 ctttagctcc tgatgcaatt ccgcatgtac ccattgcaac tttcaataca actttgcttt
     2761 cgctgtcagc tgttttcctt acctgaattt tttcttttgc ctgttcttta atcttcataa
     2821 ggtcagcaac actttttact atcggcatta agactccctc cttaatattt attatacatt
     2881 ctgggttttt gctaactgtc tgtactcttc caatattctg ggaagatcct ctttcttaag
     2941 tctgccgtga accttttcac ctatcattaa aacaggcgca agtccgcatg ctccaaggca
     3001 tcttactgcg tgcagtgaaa aaataccatc tgaagttact tcgccttctt caactccaag
     3061 aagctttttc agttcttcgt aaagaggacc tgatcctctt acgtagcagg cagttccaag
     3121 acaaagatta attggataga gccctttcgg tttcatggta aagtagttgt aaaaagagac
     3181 tacaccatat acttctgaag catgaagacc taattttttt gcaataagtt tctgcacctc
     3241 tactggtaag taacctatta tttcttgcgc tttatgcaga gtatggatca gataacttct
     3301 cttcacttcc tgatccttgc cttcaagctt aagcgtgtca acataatccg caacatccct
     3361 tagcttcatt tccatagttt cgttcatctt tttccctcct gctgatatat gagtaatttt
     3421 atccacattg atgaaaattt cacaaattca caaaacaatt tttcatcaac ttgtacaatt
     3481 atattatctt tatttgtttt tttgttaaaa aaagagaaaa catatcattg caagatttcc
     3541 taatcatttt catttttttc taagagtcct gcatatactt tatctatctt ctctttttct
     3601 tccctagcga ttctaagctt gcttttggcc tgcatcaaaa gctcaagtgc ctttttataa
     3661 agatctatag ccagatcaat atctacagtt tcatctttag gctgattttt aaaaaaattg
     3721 tttatctctt caatataaaa tatcaggtct ttgaatgaga gcttacttat atccttacta
     3781 tttatttcaa aaattttatc tgtcaattag cctgatcctc ctttctgata atctttgcac
     3841 aggcttttcc gtcaggaaag tacatttcaa cctcatcgtc aagattaaga tctttgaccc
     3901 ggctgatcaa atatgagttt ttccttatta cagccccatt gttcataaaa gcgccaaaag
     3961 gactttggcc ggttatttca gcatactgct cattaagata gttttcaaaa aaagaaattt
     4021 ggtcattaaa aaacatgcct atttcactct tttggctttc gatcaaggca tttttgtact
     4081 taagttcaga agaaatactt tcagagaatg ttgatagaat agtgtaactg aaattttttt
     4141 catacaatga aaaaatattc tcaatagaat tatctattat tttgtgaaaa tcgtcaaatg
     4201 atttattctg ataaaaaatg caattttcca gagttattct cattaaatcg gtatttttaa
     4261 gtgaaattaa atcttctatc gttttctgat aaaaatcaaa gctttctttt aagtcgtcga
     4321 ttttcccaac aaaaatttca tctacttcgt caatctgcct aacaatatct cttgcagctt
     4381 ctgttggagt ggaatacctt ttaaaactca cataatcagg gatactggta tcctgttcat
     4441 gtcctattcc tgtcagaaca ggtattattt cattaaactt ggctatatag acaccaagtt
     4501 ttacatcatc aaaatacatt agatcacttt ttgaacctcc ccctcttatc aatgcaacaa
     4561 catcataagg aatcttcgac tttattatat tagtcattgc attaataacg ctgtccacag
     4621 tttctgcacc ttgcatgaga gcaggatata gatggacaac cggtatattt ttggcagcta
     4681 taatattctt ttcaaaatct tggtaacctg ctgctgtagg tgaactcaca acagcaattt
     4741 tttttatagg ttcaagttca ttcagtgttc tttcttttat cctgagaaat cctttttctt
     4801 ttaaaagaga aagaattttc atttttttta actctatatt ggagtcgcca accggaaaaa
     4861 gagctttacc tgtcatgaca aatcttagat tgcctttcca aagagtaata ttccccataa
     4921 actcccattt tttacctaca agctcattgg gatttttaat atcaagcctt tcaagtacat
     4981 atgaggcata tgagtttggg aaaaataccg tcattgagta gtttgaagct cctgtctgct
     5041 gagaaagttc tatataaaga tcgtttttgc tgctgtactt tgcagaagaa acatccccaa
     5101 taatttttac actctgatta taaagatcgc ttgatgtaaa aattccattt atatagtcgg
     5161 caagatcctt taaggaagga aaatccatat tattcttcct ctgtttgtct gatatgcatc
     5221 ttaccgctca gtaccaccgt aaactctccc tttacttcct tacgttggct aaactctttt
     5281 attgcctgac ttacagtagt acgaaaaaac tcctgatgaa tctttgtcat ctctcgtgca
     5341 aaaaaaactt ctcggtcgcc tagtatctga agaatatttt ccagagtctg aacaatccga
     5401 ttgggtgatt caaaaaaaac cattatattt ggaaaatcaa caagagtctt ccacatccta
     5461 cgctgatttt tatctctggg aataaaaccc agaaaataaa actttgatcc tggaaaaccg
     5521 ctggctgcta tagctgttac aggagcactg gcgccgggta tgacatctat ttgaatacca
     5581 agttcgtaac atctttcaac aagttgatat cctggatcag aaacaacggg cataccagca
     5641 tcagaaacaa aaacggcagt tatacatttt tttagctcag atatggcagg ttctaacgtc
     5701 cgttcagctt tggcctcaca gaatgtaaaa agtttttttt caccgatagc ataatgattc
     5761 agaagtttta taattactct tttatcttcg gtaaagatca tatcgcattc ttttagagct
     5821 tgtaacgctc gtggtgaaat atcttcaagg tttcctattg gagttgcaac aattataagt
     5881 ttagccacgt atcacctcat aaaaaagttt tagaaaatgt tccttgcata gtccggcaat
     5941 ttcagagttt ttatacttct tcagtccggt aacatcaatc aaactttcat tggtaaaaat
     6001 tttgttcaca aattccgaaa gatcctctac aaatgtattt ttttcctcta tttccattaa
     6061 aaataatttt tctccggtct gaggcatatt atattttatc atttctgctg ggtttaaaat
     6121 ctcatctaca attatttcag gatcatactc aagaaaatat ctgtttttta cgaccaccgg
     6181 atacgtggaa agcctattaa actccacatc agatggatta agactacttt catcttcata
     6241 ccttgactga atatcaattt ctgttttaaa atttcttgtc catgaaaact tttcagaaga
     6301 aatttccagt ctgatccgtt ctccttcatc aagataccat tgaaagatta aagatctggt
     6361 ttttgctttc ctgataaggt tcaaagctac tgtctgatca tcaaaaatat acttacgttc
     6421 tttttctatc ttcattttta tcaccatctt tttcttttag catttaaaat tttatcatat
     6481 ttcatatcta taagaaaaga aaaaggacag cttctgctgt cctttggtgc ctggggcggg
     6541 acttgaaccc gcacgagaaa agcctcatat ggccctcaac catacgtgtc tgccaattcc
     6601 accacccagg cattgctcag tttctcagtt taacgttttt tattatacac tataattttt
     6661 tgtttgtcaa tatgaatttt tatttttcat ttgtagttta cttttatttc attatatatt
     6721 gcgtcatcta ccataccata aacatttagt ccaagggaaa gacttttacg gataatggat
     6781 gaagaaatat ctatgatggg actttgaagt tttatgaact gtctagtact tcccaaaaat
     6841 cctctttcat caaaaaatct tggataaaca acaagcttac acagcttata aagctcctct
     6901 gccatatacc atctgttaaa agaaagaaaa gaatcctccc ctataagcaa atacacatca
     6961 ctataactta aaagcagttt agtaacagta ttgtaagtat acgatcttcc atctaaagtg
     7021 ctttcaatat cgctgacata aaacctttcg cacggaaatg ttttccggca ccactccatc
     7081 cgcttatcgt aggctgcaat atcagatccc atcttatgcg gagatataaa agtaggtatt
     7141 attaaaaatt tttctggttt taaaagatca tatgctgctt cagcaactat tcgatgccct
     7201 acatggggcg gattaaatgc tccgccaaag accactgtca tcaaaaatca ctcaccttat
     7261 atattcaaag gcaaattctc cgcaataaat agtatctccc tcagatactc cggctcgtct
     7321 caataacttt tccaatccgt ttttttcaag tatgccaaga atttttttcc ttgagtccat
     7381 ctgaaatata ttgaattttg aaagaagctg aactatatct tcccctacta tttcatactc
     7441 atgttccgca attctgacaa cctgatactt tatcttcatt acagtctcaa ctttaacagg
     7501 ctctgcatca aacttgatct tttcgggcat acttcttttt aatttctcat atttacgtat
     7561 ttcttcttcc cttctgtctt ctattatgct gtacataatg cctttaagtc tgtcaacatt
     7621 atatccggta taacacgaga tgggaataac aggttttcct atagcctttt caattttcga
     7681 acaaatagtg acaagtttat cttctgaaag agtatccgtc ttatttaaaa ctattacttc
     7741 ttcttttaaa gatatatcct tgttaaattt ttcaagttcc tttcttatgc tataataatc
     7801 attaaccgga tctctccctt catactctga gccatcaatt atatgaacca gagcaaaaca
     7861 tctttctata tgtttaagaa acttatctcc aaggcctatc ccttcatggg ctccttcaat
     7921 cagtcctgga atatcggcaa ccacaaagga ttttccttca tctaccttaa ccactccaag
     7981 atttggaata agcgtagtaa atggataatt ggctattttt ggtcttgcat tggaaatttt
     8041 tgatatcaga cttgattttc ctacgttagg aaatccgaca atacctatgt ctgccaataa
     8101 tttaagttca agcttcaaaa cttttttctc tccctcaata cctttttcgg atattcttgg
     8161 agcctggagt gttgaagtgg caaactttga atttccccgt cccccgatgc ctccgcgagc
     8221 gacaataacg tattcgttgg gatattggag atctgctatt ttatccatat ctggataatt
     8281 atatattacc gttccaaccg gtacttctat aaccatatcc tccccatcgc atccgtattg
     8341 attttttgtc tttccgttct ccccgttctc ggccaaaaac ttctttttat atttgaaatc
     8401 caccagagta tttttctcaa gtacactctg tattatgata tgtccaccgg taccaccgtt
     8461 tcctccgtcc ggaccgccaa atggcgcaaa cttttcacga cggaaactta ttgctccatc
     8521 tccgcctttt cctgctgtaa tctctattat agcctcatcc aaaaaatcaa actccatcta
     8581 tttccctccg ttatttcctt ttttcccaaa agtttttttt atacttctta cattaaatcc
     8641 aaaatcttcc ttttcttcat tgtctatcgg ttcaatatgt attataacat ctcttatata
     8701 tggattttct ttttttattt cattttcaat attcttaaca atcatgtgag cttgatgtac
     8761 agtcatctga ggatcaattt ctatatgcat ttcaacaaaa taaatatatc cggacttcct
     8821 aactcttatt ctatgaggat tttttacttg atgaaaactt tccattcctt ttaaaatatc
     8881 gttgtataca tctcctagtt ctttggaacc gtccattagt tcattggaag actccatgaa
     8941 aagagagatt ccgactttaa agataaaaac tgaaacaaat atagccagca aggcatctat
     9001 ccaccatatt ccggttataa atatcaaaaa aactcctgca agaaccgata tcgaagtaaa
     9061 tacatcatta cgcatgttaa gtgcatcggc aacaaatgaa gaactgttta tttttttccc
     9121 tattgcaagt ttatatctgt ataagaaaaa ttttgtaaaa accgatatgg ctgaaacaat
     9181 cagtatcaaa gaaacatcct ttatttcaga ctgttttccg ccaagtctgg aaatagcact
     9241 tgaaagaact tcatatcctg catatattac tataagagaa agaatttttg ttatgatagg
     9301 ttcaattttt tcatgtccgt aaggatgaga ttcatcggca ggcttgcttg aaagttttgt
     9361 agcaataagt gtaagaaatg atgtaaaaat atctgttaca gtatcaattc catcggctgt
     9421 aatcgcaaga cttcctgtaa aaaaaccaat tgaaagcttt aaaaaaccta aaactgcatt
     9481 tccagcaatt cctattaacg aaccttttct tgccatactc tctctctgtt ttatagtaat
     9541 tgatttatta tctgtcatat cttcccctcc cggtttttga ttttttaaga ccaaaaaagt
     9601 tttcagagtg gaatttttat gttgctttct cttagatgaa cgctgtaatg ctgaattttc
     9661 ttttcaatgt tatattttat taaacacaaa aagcaacaca tatgtcacct ccttttaata
     9721 tttactcgac atatataagt gagattatac taaaattaca tagaataagt caagaaaaaa
     9781 ttatttgttt tttagccttg tttattgtat aattaatttg gaggtgagat acatgtttgt
     9841 aagcattaca aaggattcat ttatagccgc cgaaagagta cactcaatta ttcctcagga
     9901 ttttatacag ttcagaagaa tgaaaaagat gtttcagggc gatgatgtcc tttctgaagg
     9961 cgaaaggcga ggaagggccg atgcctcaag ccaggaagaa aaacttgatg aggaatttat
    10021 taaaggcagt acaagcctta tagatattac ttatggcaaa agtgttgaaa ccataatatt
    10081 tatggacagt ggtcatctta tattaacttg tgtggaagcc aaaaaaataa ttgacaaagt
    10141 aaaaaaatca aggaggggcg aatcatgagt attccaatgc cggttgtaat agaatctgac
    10201 ggcagatatg aaagatcata cgacatttat tccagacttt taaaagacag gataatattc
    10261 ttaggcactc ccatagacga cactgtggct aacgttatta tcgcccagat gctctttttg
    10321 gaatcgcagg atcctgacaa ggacatatca ctttatataa actctccagg aggagtaata
    10381 actgctggtt tggctatata cgacaccatg cattatataa aaccgagcgt atccaccata
    10441 tgtcttggac aagctgcatc aatggctgcg gtactgcttg ctgcgggtgc taagggaaaa
    10501 aggctttctg tacctaacag tagaattatg attcatcagc cttctggcgg catggaagga
    10561 acagcagcag atatagaaat acagaccagg gaaattctta ggatgagatc acagcttaat
    10621 cagatactca gtagccatac cgggcaacct ctgaaaagaa ttgaaaaaga tattgagaga
    10681 gattatttta tgtctgcgca ggaagctctt gattatggtc tcatagacag ggtttttgaa
    10741 actaagaaga ccgatgataa aaacaataaa taatcggaat gaaaagacgt ggccgctatg
    10801 cggctatgtt ttttaattag ggggaaagca tgaaagatca agaatttact cctatgatta
    10861 ggcagtacat ggagataaaa aaagattatt ctgactctat actgcttttc aggctcgggg
    10921 atttttatga aacttttttt gatgacgcaa aaacgatgag cgataccctc caacttactc
    10981 tgacaaaaag agccggacat cctatggcag gcattcccta tcatgcgctt gataattact
    11041 taaaaagact gatagataat ggtttcaaag ttgccatctg cgatcagatg gaggatcctg
    11101 ctttggcaaa aggaatagtt aagagaagtg taacccgtat tcttacaccg ggaacagtgg
    11161 ttgaggattc catgcttgat gagggaaacc gtttttcaat tctaattgat aaaaaaaaca
    11221 actttacctt ttcaatattt gactttacta caggtgaggt atacctcgac agtatggata
    11281 gtacagacga agagatgatc gattttatgc tttcctacgg aattgttcag attctgcttt
    11341 cttctaatct aaaatatatg ctgaaaatta taaaagaaag acgtcccgga atatactgcg
    11401 aggttgtcga agattggatt ctctcgggaa attatgatag ccaccttaaa tcagtttatt
    11461 cggtaagttc tttggatttt ctggagtttt cctcttgtga gcttgtcctg ctggatggtg
    11521 ttttcaaata tcttgaaacc acacagttca aaacaataaa acattttcac tttcccaaaa
    11581 aattcagaag cagtgattat atgttccttg acagcaatac aatctataat ctcggtgtag
    11641 taccacagcc agagcaaaga gggaaaagtc tttttgatac tttaaattac tgtaaaacca
    11701 atatgggaaa gagatttctt gctgaaaacc ttaagcgccc gctcacagac agcacacata
    11761 tagaaaaaag actaaacgca gtacagatgt taacctctga taaaaaactt ctaacggaaa
    11821 ttcaagatag tctctgcggg ataatggatc ttgaaagaat ttcaagcaga atctcaattc
    11881 aaaaatcata tccgaaagat ataaatgcac tttcatcatc aattgaaaaa agttctgagc
    11941 tctcatacat cattatgcaa aatataggct tatatgaaat ttttggcgcc atacctaagt
    12001 tgatggatgt ttatgaaaaa ataggaagat atatctttga agatgcctct aatgatgttg
    12061 gaagcggaat ggtcataaaa gatggaattt ccagtgaact tgatgaatat agaagtattt
    12121 caagagatat cgactctgtt ctcagggaac ttgaaaaaag agaaaaagaa acaaccggtc
    12181 tttcaagctt aaaaatagga agaaacagga tctatggttt ttatattgaa atatcaaagg
    12241 ttcaggcaca atctgctcct ttatcctaca cgagaaaaca aactctgaca aactgtgaaa
    12301 gatttgttac cccagaactt aatgaacttg aaaaaaaagt tttggtcgca caggagaaaa
    12361 taaggaagat agaaacttcc atttatgatg aatttatagt ttccctatct tcttacctaa
    12421 aaagaataaa agaactgtca gcagttatct ctgaaattga tcttgcctgt tccttatctc
    12481 aagctgccat cagaaactct tatataagac ctttcttcgt agaaaagcag gaagaaattg
    12541 aaataataga gtcaagacat ccgatggttg aaaaaactgt ggataatttt caaaaaaatg
    12601 attttgcgat aagcggaggg aaaaacttta ttattataac cggtccgaat atgagcggca
    12661 aatcaaccta tctcagacag ataggcttga taagcataat ggctcagata ggctcgtttg
    12721 ttccggcaca aaaggctttt ataaccattt atgacagggt ctttacaaga ataggtgcca
    12781 aagatgatgt cgtatctgga aagtcaacat ttttggtaga gatgcttgaa atgtctggaa
    12841 tcctaagcaa ctcttctcag aaaagtctta ttctactgga tgaagtaggt aggggaacag
    12901 gaactcttga cggaatagcc gttgcctggg caatatcgga gtatcttttc caggttatag
    12961 gagcggttac actttttgcc acacactata ctgaacttac cataatgtca gaaatatatg
    13021 aatgtgttgc aaacaaaaga gtacgaattt tagaatcaga aaacggtatt gtctttcttc
    13081 ataaaattga agaaggaagc agtagcagtt cttatggaat tgaagttgct aaacttgcag
    13141 ggtttcctgc agagataact caaagagctt tagagatact ggaacagctc tctgataaag
    13201 cagatctgga aaaccggctt aaaagaatca aaaatataaa acgccgcaaa tatcggcttg
    13261 acgagaacca attaaaaatg ttctgatttt tatgatataa ttactcttag attgatttta
    13321 caggagataa cttgatggaa gacaccctat tgaaaaagaa gcttgaagat ataaggcttt
    13381 atatgcgaag taactttttg gactgtttta tagttattaa ctatgaatcc tcttccagaa
    13441 gtacaagcag gtttcttacg ggatttcagg gcacattctg cattatcttc atatctcaag
    13501 aagactttga tttttataca gacagtagat atctggagta tgtcgaatac ggatataaaa
    13561 gtctgaacgt gaagatatac gacggaaatc tcattcagct gcttaaaaag ctcctatctg
    13621 ctttcaagcc tggagccaag atcggttatg aagcctcaaa tataagtgta cgtttttaca
    13681 aaagattttt tgaagtattc aaaaattttt cttttgtagg ttgtgaagat atctttcttt
    13741 ctctaaggac taaaaaaaac gcgtctgaaa tagataacat aaaaaaagct gttattattt
    13801 ccgaaaaatc atatctagaa tcaattgaaa ggttcgaata tggaatgtct gaaaaggatt
    13861 tctcagctct tttggagtac aggatgcgta tcaacggtgc agattcctgt gcttttgaga
    13921 caattgtggc ttctggaaaa agaagctgta tgcctcatgg atatgcttca gacaagaaaa
    13981 ttgagaaagg cgatattgta attgtagact ttggtgcatg tttgaatggc ttgaattctg
    14041 atataacccg tgttttttgt gttaataaaa tagatccaag gtataaaaag gcttacgatg
    14101 ttattttatc agctcagaat ttagctattg aagctagcag accgggtatg ctgagcagtg
    14161 atttggatgg tgttgcacgc ggaatcatta aaaagtacgg tctcagcagt tattttactc
    14221 atactcttgg acatggtgtg ggctatgacg ttcacgaaga accaaaaata agcaaaaaca
    14281 gttcttatat ccttgaaagc ggaaatgtat ttaccataga accaggagta tattttccgg
    14341 gcgactttgg tgtacgagta gaagatacta tacttctgac agagaaagga catcagaact
    14401 tctgttcgct cacaaaagag ataataaaac tctagtttga ttgtctcttt aaaaacaaaa
    14461 atctaaaagc actgaggagg gaaaaaaatg ccagtaaatc aacataatac atttggagaa
    14521 ataagtattt ctgaagctgt tttaaaagat attacactaa agacggttga aacttttctg
    14581 aaagcagaaa aaatatatac agataaagta aaaagagatc ttccaaaaaa cgtccgcatt
    14641 acagcagacg agaacgggag tgtttcaata ggactcaaaa taacgggaaa gtacggtgaa
    14701 aacttaattg attttacaaa aaaattgcaa atactcgtta aggaagaagt agaaaaaatg
    14761 tctgagataa atgtagcaac aattgatata actattgaag aacttgagta tcctgaatct
    14821 gacgatacag aggaacatga acttgaagaa gctcaggaag aacacaatgg cgataatgga
    14881 gaggaacagg ctaacaaata ataatttaag aaagcgagtg tcagaatgga aaccccttta
    14941 cggattctta gagaatgtgt tgtagaaagt acttttcaaa ttaatttttc cgacgtgtcg
    15001 caggacatgc ttattttttc tttggaagac agtatgaatt ttaaaaaact tcctaagaaa
    15061 tattattctg tggcatacga gtatgtcgta gggattaacc agaacaggca gcttatagat
    15121 gccattatag aaaagcatct tgaaaattgg actttttcta gacttggtaa tatagaaaaa
    15181 tgtattctgc gcatgtctgt gtttgaaatt cttaaaaaac ctgaaatacc tttaaaagta
    15241 atctttgatg aatcagtaga gatatcaaaa aaatactgtg acgatgaatc ttccaaattt
    15301 gtaaacggaa ttttggacag aatttccaga gattctgata taataagcca taaggccagt
    15361 gattgaaatg caagagcaac cgctatacaa gcttttaaga cagatggatt acgatcagtt
    15421 ggatgaactt gcgcaggaaa tacgccagta tataaaaaaa gtggtatttt ccaacaacgg
    15481 tcatctggca tcaaattttg gagttgtaga actcaccctg tcattgtaca gggtttttga
    15541 tccttcacag gatgtagtta tatgggatac cagtcatcaa tcatatgtcc acaaactgct
    15601 taccggaaga tggaactctt tcagtacttt acgttctctg aacggcataa gcggatttac
    15661 aagtataaaa gaatccttct atgaccgttt tggaactggg catgcaggca catcaatatc
    15721 tgcagcattg ggatactctc ttgcagattc ctacaaaaac aataaccgtt ctgtaattgc
    15781 ggtaatcggt gatggttcga taaactgtgg aatggctctt gagtcgttaa atcaactaaa
    15841 gtatcagaat gcaaatataa agataattct aaataataac gacatgtcta tctccaataa
    15901 cgtaggagca cttgccggta ttttttctcg tttaagagcc aaacgcgagt attatcagtt
    15961 taaacagttt gtaaaggact ctttgagcga agcgcctttc ggccaggatc ttgaaattgt
    16021 tctcaaaaaa attaaggatt ccataaagta tagcgtttac agaagtcctg tgggatactt
    16081 tgaagatatg ggcattaaat attttggacc agttgatggt cataatataa gtgagttgga
    16141 tgtagctctt tctctgctta aggaatacaa cgacagccct gcgatacttc atgtaataac
    16201 taaaaaggga aaaggtcttg aagaagcaga gaaaaatcct actaagttcc acggcgtttc
    16261 aaaaccttca gaaaacaaaa atccttcgta tagtacaata gtaggcaagg ttttggctca
    16321 tttaaaggga tatgatttca tatcttttac tgctgctatg tccgatggga ctggacttga
    16381 agagctaaaa aaagtagctc cgggaaaagt actagatatg ggtataaccg aaccaagtat
    16441 agtcactgct gctgcagcat tttctctcaa tggtatattg ccagttgtag ctatatactc
    16501 gacttttatg caacgggcat ttgattcaat agtacacgat gttgccttac aaaacgcacc
    16561 tgttcttttc ctgcttgaca gagcaggact tgtcggagaa gacggaccaa cccaccacgg
    16621 tgtttttgat atttcttttc tcaggctcat acccgatatc aggatattaa ctcctctgga
    16681 cggtcaggat cttgcggaca tgatatatac agcggttgta aacggaataa aggaacctac
    16741 ctttatacgc tatccaaaag aatatgaaaa caataccatc cagaatatcc ttgacagttt
    16801 gaagactgtc aacgaaaaat ggatatatat taaaaaaagt acttccaaaa attatattct
    16861 tggagtagga accatatgca aaacactcaa agaatgcgta aaagactacg atataaacgt
    16921 cattggagta cgaagcataa aaccccttga tacggcagtt atgcaggagc ttactcaaaa
    16981 agcagaaaac atacttgttt ttgaagaggg ctctttaaag ggcggattca atgaagagat
    17041 atttaaacta tatggaaaaa atgttttgcc tttcggaatc aaagattctt tcattcccgc
    17101 cggttcgcgg caggaacttt tgaatatgtg cggactggat catcagaaaa taaaagaagc
    17161 aattgatgct tttataaggt caaaggagag ggatatggca tgaaaaaact tattgtgatg
    17221 tcgttattgt cctttattat ttttatatcg gcattttctt tcgatatact tgtaaatacc
    17281 agctattcat tcacacttga cgcaactgtt cagacttctt ctggcatacc aagcgcttat
    17341 gataggagtc agaaactcaa ctatacaggt ataaggataa gctatcttat tcctactgag
    17401 gacagtaagg aaattcttta tggtccgtat attggatttt tttacaatct atttgacaaa
    17461 cagacaggat catatcccca gcttaatgca gatgtgaaag atatttccta ctccatgggt
    17521 ctaaatgttg tttatacctc caagcttttt caaagcataa attttgacgt atgtgcatat
    17581 ggaggagttc atactgatga taattttaaa acatactcca cagagatact tatgagtggt
    17641 ggatttaact gggattttct gtttataaat gccggatacg atattcgata ctactatgtg
    17701 aataaaaata ataccattgc tgttaattct attcctataa ccgtgggggt gagttttaaa
    17761 ttttgagtaa tttcagagca gatcttgaaa tagaaaaaat attcgaattg atctccaatt
    17821 tttgtgttac tccatacgga aaagattatt tttataaaaa tatcgtccct ataaaagatt
    17881 ataatatatt aaaacgtgaa actatccttt cacaggacgt ttttgatatc tataaatccg
    17941 gaagggagtt tgaactttat ggcctggctg atataagaga gattctagga aaattgaagc
    18001 agggttcttt tcttgaacct atcgagttca gaaagattgc taattttctt aatcacctta
    18061 atgcagcctt tccaaaaaac gaagaatttt acactgaaag acttctcatg atacttcaaa
    18121 gtattgtttt gcacaaagaa tacgttgata aagtttataa aactgtatcg caggaaggtg
    18181 aaattatgga ctctgcttcg gcagagcttt cgcgtatacg caaggatctt aacaagacac
    18241 gagtccttgt aaattcaaat ataaacgctc ttgcccagaa gtacgccaaa taccttgcta
    18301 tagataagcc cgttatacgt aattcaagac tatgcttagt agtgagaggc gagttcagag
    18361 gaaatgtacc aggaatgctc gtaggaaaat ctgattccgg tttttcgctg tatataga
//
LOCUS       DAOSHR010000019        17759 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_2581_length_17759_cov_5.875904, whole genome shotgun sequence.
ACCESSION   DAOSHR010000019 DAOSHR010000000
VERSION     DAOSHR010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 17759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17759
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2581_length_17759_cov_5.875904"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="PLU28_01920"
     CDS             <1..363
                     /locus_tag="PLU28_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG33854.1"
                     /translation="ASFWTHAITTNAQGERLTASIKFLKFITSKQAMELWLKNVGELP
                     ANPEIANKYSEDKLYGPFLKGLAYAHATFFADEVGQRNVILEAIDKVRLNNTDPSVAF
                     KEAADKEQKILDDFWASK"
     gene            421..1308
                     /locus_tag="PLU28_01925"
     CDS             421..1308
                     /locus_tag="PLU28_01925"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOG33855.1"
                     /translation="MTLKQKKVVTAYTFLAVPIVFFLFVRFFPMVYSMAMSFTDWNLL
                     KKNINFVGLNNYIKIFNDPVFVTSLFNSLKYILFGAPLVIIISLLMALLLNKIRKIQW
                     LYRLLYVMPYITPMVAVSWVWRWMYQPLPLGVFNNALVALGMDVQPFLSSPSQALFCI
                     VVTTVWVEFGYCVVIFLAGIQTIPEEYLEAARIDGASRWNITMKITVPLLRPITLFLV
                     VTESIQFLRLFTQVYNMSFQGTGGPLDSTKPMALYIYHKAFVNFDMGVASSASFVLFL
                     IIMFITFLQLRVFKDKVSY"
     gene            1319..2140
                     /locus_tag="PLU28_01930"
     CDS             1319..2140
                     /locus_tag="PLU28_01930"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOG33856.1"
                     /translation="MTRTDKRLTIFAYVVLSIIAITTIFPFFWMVSSSFKDASQIYDM
                     KLLPSSPTLDNYKNIFEFSLFPKWFLNSFIVAACTTLSVLFFDSLVGYTLAKYEFPGK
                     KVLFTMILSTLMIPTEMLVIPWYMMSKNFGWLDSYWSIMFPGMISAFGIFLMKQFMTS
                     VPDELMDAARIDGLSEFRIYWNIVLPLVKPALATLAIFNFIGNWNAFMWPLIVSSNSE
                     MYTLPVGLAYFSSEASFQWEQIMTGATVSMIPLIIVFLIFQKQIIKGIALSGLKG"
     gene            2145..3041
                     /locus_tag="PLU28_01935"
     CDS             2145..3041
                     /locus_tag="PLU28_01935"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOG33857.1"
                     /translation="MKIIDSHVHFPAIDFMGDGKKTHPVQHHPWLEKERNKWKQAWNF
                     GDEQPVTDEGKAIEDWYFQALKHNLDRVVFVTAGGNMLAQKIVEKHPDKFIAYAHNDP
                     STKGAESELERALKYGGLKGYKILGPAVETPLNDKSLYPVWEVAQSYDVPVLIHFGIM
                     GGAGGTAYHVNINPLIIHDVAKAFPNMKIIIPHFGCGYVFETLNLCWACPNVFIDTSG
                     SNQWMRWMPYDVSLESLFRKYRETIGPERIIFGTDSSWFPRGFTSAYLDEQLRAMIYT
                     GFSADDIDKVLYANIAQLLRIR"
     gene            3048..4484
                     /locus_tag="PLU28_01940"
     CDS             3048..4484
                     /locus_tag="PLU28_01940"
                     /inference="COORDINATES: protein motif:HMM:NF012432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HOG33858.1"
                     /translation="MAFNPIYTEKVLNPSYKNWKDLFFSHSVYLHRAHLIMLLKTGII
                     PHETARKLKYAIDSVAQKKNFPKDIPPGSEDLYFVFEKQMEEVIGQDTAGFLHTARSR
                     NDMDTTVFRMFVRTRLMDFLSCCINLLSVIQDKVYKQGDTPIVLYTHGQPANPSTLGH
                     YLSAFALDLCEDINELFSALRIVDRSSMGACAITTTGFNIDRKMVSDLLGFESPVSNS
                     YQAIVTSHWLTYPADAVRKILNDVTRFSADLSHKASCEVGMLLFPDELVQISSIMPQK
                     RNPVIIEHIRIQAGMAMGIMNMINELFHNAAYQDINEVADAPVKEFENSLELAESVVA
                     LMSQTIKNIDISRQRVSEIALSSGTTTTELADELVRLYGISFRQAHSITAGFVRSGYD
                     KDALRNIFQEKTDRSLKLSDKEIDEILSIERFISVRKVSGGPSEEGMEVVLKELEKYI
                     SAFSFSLDKKEKDILSAQQNLKTEYDII"
     gene            4557..5387
                     /locus_tag="PLU28_01945"
     CDS             4557..5387
                     /locus_tag="PLU28_01945"
                     /inference="COORDINATES: protein motif:HMM:NF017493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HOG33859.1"
                     /translation="MISKELIKKLFIQDLIKTNNLMSEDVKKYLESYDGRFSSILKEN
                     CTIALEKGLPLCQDTGMVEVFAYVGIKARIDEPLQNILNEAVKETYISNPYRYSTVAD
                     PLFDRKNQKNNTPCIVHTELTDGDSITLKYIVKGGGSENLSALFMLRPSAGPQEIIST
                     VLTHIENNGARGCPPLNIGIGLGGTSDKAMVMAKLSLTEPFEKRNPDPRYAELEMTIL
                     SKINDLKIGYQGLGQGISAYSVHIKEYPTHIATLPLAIAADCYLSRKGSLCIGGGQND
                     "
     gene            5380..5880
                     /locus_tag="PLU28_01950"
     CDS             5380..5880
                     /locus_tag="PLU28_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HOG33860.1"
                     /translation="MTRDDIKLLRTGQELLYTGELLIMRDAAHKKLYELWQKKEKFPF
                     DISNHIVFYAGPAKKVPEQPIGAIGPTTSARMDSFLEMLFENGVIATLGKGKRSAVNR
                     QMCQKYSCAYLVTPSGAAAALSQRVQSIRVVAYEELLSEAIQLITVHDFPMIVATDTT
                     GEELFM"
     gene            5949..6599
                     /locus_tag="PLU28_01955"
     CDS             5949..6599
                     /locus_tag="PLU28_01955"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOG33861.1"
                     /translation="MNILCISCSNVAGYSETGTSANVCRIIKKLVEKQAGASCTVNIE
                     KLHDYNPAACNMCMACAGKNECVYDSHFNELFQKICQADLVFYVIPHYSIIPAKLTIV
                     FEKMQEICYVNGCRDRNYIFPLIGKRAALIGHGGSPKEMEPYYRTYILNTVASVLKSV
                     GVKIATYGEQNDNGVVFGVTGYIKKEGKDVPDMQIDWIQVEEMISGYVSETLYNII"
     gene            6687..8381
                     /locus_tag="PLU28_01960"
     CDS             6687..8381
                     /locus_tag="PLU28_01960"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG33862.1"
                     /translation="MNGTNSKIPESFDELEYLKLIFDFNPCLICITDLETGVFLDANK
                     SFMMKLGYQKHELLGSSPSKINLFKRRSDLDILLEQIKEKKEVENYEILVQDKQGKPL
                     YISLSSKIILLNKRPCILSAGIDMSDKKIMEDEFNESVFYSYSLNMLINEISTELIQC
                     RHEDIESTIDITLKKIGSFSNVDRVYIFEFSDGMKCLSNTYEWCEDGIKPQKEQLKNI
                     SSTTIKRWIERFNEKKEVYIYSVSEIPDEYALEKESLASQDIKSLLVIPMIYSGKLIG
                     FLGFDSVKDYRFWDQSIINMFSTIAEILAATLIKRDYEKELISARDIAQMADKRKGEF
                     LASMSHEVRNPVSVIVGLSELLNLRIKDPKEKEYINGIMVNGRNLLKLLNDILDLSKI
                     DAGKTILHIQKIDLGSFLGEFEQIYGMQFKNKNISFILKRHPDLPQNIWTDEVRLRQI
                     LMNIIGNSEKFTYEGYVALEAKFLKVSNEYGDMCFEISDSGIGISQENIEKLFEAFSQ
                     NPNQDRKYGGTGLGLSISKKLVELMGGHISVQSTQGVGTKVSITLHGIKYSDTARN"
     gene            8406..9512
                     /locus_tag="PLU28_01965"
     CDS             8406..9512
                     /locus_tag="PLU28_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG33863.1"
                     /translation="MNKLVFIQTVFFLSMLLISCSAEKDIKPFSITSEKNVIVKPMPL
                     KYLLSDTLEEIYLTQVATNDIYDRKTYESIAKNFPKIKEHFEWMEETYLLFSEDMKQE
                     LELLYGKRRKVNPWYLVDFVTNLYDEASFDEIMAYYRSISFFGKYSFMQGQTKESILK
                     LFPYFYNNFFREYIEKNQSYYIELAQDMNRRIEDDPFDIITYMQKMSGINFSRKYKAV
                     FYYGYRPIGAFGSVYEDFYISTIPPTVKEYSTLYSVPFHEFSHELFKTFTRATPFTDA
                     CKSKLEDKQFYDYWINKGLNNSYGTWSSWCEENLVEGFSKYLYFVRYGYMNQQPTYYY
                     DMEFYEFLKNTDFHIRLSNKETTLERACIEFLTK"
     gene            complement(9548..10699)
                     /locus_tag="PLU28_01970"
     CDS             complement(9548..10699)
                     /locus_tag="PLU28_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG33864.1"
                     /translation="MILRKLIMVGIIALMVFAGGIPLLNQRNFKEVAVKGPGITEVFK
                     LSKYFEGIKGTIADTDVFVFKGQEEGGKVLLMAGTHENEISAMLTAFLFLENLTVQKG
                     TVYVIPHFNYSGSLGTQPGGGFPLYYSFKTPWGEKKYRMGDRGLQQLDQWPDPDVYVH
                     YPDKQLLSYIDARNTNRSWPGRPNGFLAERITYAAMQMMKEEKIDVAIDLHEAEVMFP
                     VTNCIVVPDKSRQIALGVSMSVKAKEKFENHLEPSPSSFRGLSHREIGDYSDTLPFLF
                     ESPGPGLDQITGPKTNELLMTGKDGFILEASKYGILYVPYDETGWSMEKRVGQHASLA
                     LEVIKQFSRRNPDRALEVSGPRYPEIVEKGLGSFFTDPSTVDPSLIIYQ"
     gene            complement(10714..10869)
                     /locus_tag="PLU28_01975"
     CDS             complement(10714..10869)
                     /locus_tag="PLU28_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG33865.1"
                     /translation="MMEIINVVYYIIVAVLAVFLFKNLFKEKNLQKELMYVLTILPFL
                     LRLFRFK"
     gene            complement(10881..12182)
                     /locus_tag="PLU28_01980"
     CDS             complement(10881..12182)
                     /locus_tag="PLU28_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG33866.1"
                     /translation="MSTSSLYIQTILALVVMAGAYVVSKLFKFSTEISLFFAVVAGAI
                     YGGAGFDTFRHLAEGSIAYFDIGLIFLFATLFMNIIKESGGTDYIVRGIIKGFYNKKY
                     LMLILLMVVMLVPGSITGAGSISVMVVGGTVSSALVAMGLGLDTVSAIIFLTAAMSAV
                     APPINVWAMMTCACTAIPYVGFTFPLLIPVIVLGLFSVLFLGRKAKNIDRDAALKNIP
                     EPKGLSGWSVGIPFLVLIFLLTAPRIWPFDFPVMGLPLNFAISALVAFAMNFKRIKIL
                     KVIESTIDQVIPLLATTAIVGMLLQVISFNGVKGLISMTVVTMPVVLLFILLPAIIPV
                     SEAILTYGSAALIGAPLVWMLNSRGYNAVLAIAGLSFMWALGDALPPTALIGRLSVST
                     AGYTGKYSSFLKKSIVPWIAITAVGLLMIIFSNQLSFLMFS"
     gene            complement(12209..12853)
                     /locus_tag="PLU28_01985"
     CDS             complement(12209..12853)
                     /locus_tag="PLU28_01985"
                     /inference="COORDINATES: protein motif:HMM:NF040376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6305 family protein"
                     /protein_id="HOG33867.1"
                     /translation="MKKLALTIVILSLVFASVSFGAQTPFLKYPTVITSAGQSSEAEI
                     LTYVCDEMGIKYDYCDILSEKEFAAGVGLGGAKSGPGKHVTVTSTQAKGTKYESLWLV
                     LGASLKGMGASGLSVDDEVSRLKKLINYAKDKKITLIALAIGGEIRRGLPGSLNEQII
                     DAVAPFCDVIITTKDTNKDGKFTQLAKSKNLKFIEIDSQESLLELVPEIFITAK"
     gene            complement(12929..14578)
                     /locus_tag="PLU28_01990"
     CDS             complement(12929..14578)
                     /locus_tag="PLU28_01990"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF015536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HOG33868.1"
                     /translation="MKKLLLTVLMVAISITFAIACTTIAVTKGASVDGSTSVTHSCDS
                     GDCDFRLIYVPAMDHAPGSRRNVYPYVEPFPRYNGKDFGPNYDLPGYPLTEALGSIEQ
                     VPHTYAYFMGSYGIMNEHQLSIGECTCSAKVYAQPKAGERIFDISSLSMVALERCTTA
                     REAIKLMGELGVRYGYYGWGETLTIADTKEVWVFEMCGDPSGKSALWAARKVPDGEIF
                     VEANEFRIRELPENDPNVMFSPNLKEITKTAGWWDGKGDFDWLRTVSTGEYTHPYYSL
                     RRVWRIMDRLAPSLKLSAWVEDGFTKAYPFSVKPEKKVTIADVIDLYRDWYQDTEFDL
                     SKGIAAGPFGNINRYSGSSKLVKGDWERAISIFRASYTFVTQSRDYLPDPIGAVLWYG
                     ADCAHSTAFVPFYCGMSDLPKSYQIGNQKELDRNAAWWAFNYVSNLADLKFNYMIKDI
                     QAKQREVEGKEFIMQPIIEQTALELYKKDPAQAISFLTQYCSDNADSVYKQWWDLADY
                     LMVKYQDGYVNIPNVATSVGYPDWWLKAVEIAPIQYQKPAK"
     gene            complement(14594..16243)
                     /locus_tag="PLU28_01995"
     CDS             complement(14594..16243)
                     /locus_tag="PLU28_01995"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF015536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HOG33869.1"
                     /translation="MKKLLTVLLVLLSFAAAVIACTTIAVSAGASVDGSVSVTHSADS
                     GDYDFRIISVPAMDHEKGAKRAIYPFGEAYPRYVGSDLGPGYEMAEFPKTEPLGYIDQ
                     VAHTYAYIDGIYGIMNEHQLAIGECTASAKVYAQPKVGERIFDIAALSRIAMERCKTA
                     REAVKLMGELAEKYGYYGWGETLTVADTKEAWVIEMCGDPEGKSALWVAKKVPDGEVF
                     VEANEFRIREIKVNDPDMMWSSNLFEATKKAGWWDGNGELDWLRTVSTGEYSHPYYSL
                     RRVWRIFDRIAPSQKFSAWVEDGFTKAYPFSVKPDNKLSIADIIDLYRDWYQGTEFDL
                     SKGLAAGPFGNINRYSGSSKFVKGDWERAISVFRASYTFVTQSRSYLPDAIGGVLWYG
                     ADAAHSSAFVPFYCGMNNLPKSYQIGTQKKVDRNAAWWAFNWVSNLMDIKFSYMLTDV
                     QAKQKEVEGKEFAMQPMIEKAAQELYKKDPALAKEYLTDYACANADSVYKQWWNLADY
                     LMVKYQDGYVNIPSVAKSVGYPDWWLKAIEFAPIQYQKPVK"
     gene            complement(16613..17116)
                     /locus_tag="PLU28_02000"
     CDS             complement(16613..17116)
                     /locus_tag="PLU28_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG33870.1"
                     /translation="MIFSAALLFAQSERLYEYVLDIWSKNKTEYQKTFSIFSNDYYVF
                     SQIPEIGFSAEYLLEESSGELLVSVKYTDYSKIPAVLEADLCRNGFNEDLSELIVYDG
                     ICNSIDEQDVNESQRFFVMSGSSEPCDLKDSAYKIGSFEYEGKWFVVKSKTDKNNNLH
                     FIVSFKK"
     gene            complement(17152..>17759)
                     /locus_tag="PLU28_02005"
     CDS             complement(17152..>17759)
                     /locus_tag="PLU28_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG33871.1"
                     /translation="QSSWRGTLITTTFYYTRCMTMNNFYNFDNLGNITNIFGDKVGIF
                     DRYGYLYVVDKVLPFACIQPYGVITSINDENIVIGSIGKNEIIMDEDKDPFGACAHDK
                     MSYESFGFEADEIIPITEKYRCITIDILYPDFMDCYNKEAKENKDSVHTDAEIVEETE
                     KQAKEEIYEYDFSDTPDELFYEEKVKAQNIIESINPLEEYR"
BASE COUNT         5454 a         3623 c         3360 g         5322 t
ORIGIN      
        1 gcatccttct ggactcatgc tattaccacc aatgcccagg gagaaagact gacagcttca
       61 ataaaatttc ttaagttcat aacttcaaaa caagctatgg agctttggct caagaatgtt
      121 ggagaactgc cggccaatcc ggaaattgca aataagtact ctgaagataa gctttacgga
      181 ccgtttttga aagggctcgc atacgctcat gcaacttttt ttgctgatga agtcggtcaa
      241 cgtaatgtga ttcttgaagc tatcgataaa gtaagactta ataacacaga tccgtcagtt
      301 gcttttaaag aggccgcaga taaggaacag aagatccttg atgacttctg ggcatcaaag
      361 tagaaaaatc atccgcggag cttcataggc tccgcggatt ttacagggag gatcagttaa
      421 atgactctta aacagaaaaa agtagttaca gcttacactt ttctggcagt gcctatagtg
      481 ttttttcttt ttgtacgatt tttccccatg gtctactcta tggcaatgag cttcaccgac
      541 tggaatctcc tcaaaaagaa cataaatttt gttggtctga ataattacat taagatattc
      601 aacgatcccg tatttgtaac ctctcttttc aattctctca aatatattct tttcggagca
      661 cctttagtca taatcatatc tcttttgatg gctcttttgc ttaacaaaat aagaaaaata
      721 cagtggctat acaggctttt atatgttatg ccttatataa cacctatggt tgctgtcagt
      781 tgggtgtgga ggtggatgta tcagcctctt ccgttaggag ttttcaacaa tgcgcttgtt
      841 gccttgggaa tggatgtcca gccatttctt tcaagccctt cacaggcgct gttctgtata
      901 gttgtaacta cagtatgggt ggagtttggc tactgtgttg ttatctttct tgccggaata
      961 cagaccatcc ccgaggagta tcttgaagca gcaagaatag atggggcaag caggtggaat
     1021 ataaccatga aaataaccgt acctcttctc agacccataa ctctctttct ggtcgtgaca
     1081 gaaagcatac agtttttaag actttttact caggtatata atatgtcttt ccagggaaca
     1141 ggaggtccgc ttgactctac caaaccgatg gctctttaca tatatcataa agcatttgtg
     1201 aattttgata tgggagtcgc atccagtgca agctttgttc tgtttctcat aataatgttt
     1261 ataactttcc ttcagcttcg tgttttcaaa gacaaggtaa gctactgaga ggtgatgcat
     1321 gacgcggaca gataaaagat taaccatctt tgcatatgtt gttttaagta taattgccat
     1381 aactacaata tttccgtttt tctggatggt atcttcttcc tttaaagatg cttcccagat
     1441 atacgatatg aagcttttgc cttcaagtcc tactttagac aattataaga acattttcga
     1501 attttcccta tttccaaaat ggtttctcaa cagttttata gtagcggcat gtactacatt
     1561 atccgtactt ttctttgatt ctttggtagg atatactcta gcaaagtatg aatttccggg
     1621 taaaaaagtt ctttttacca tgatactcag cactcttatg atacccacag aaatgcttgt
     1681 tattccatgg tatatgatgt caaaaaattt tggctggctt gattcatact ggagcattat
     1741 gttccccgga atgataagcg ctttcggaat attcctgatg aaacagttta tgactagcgt
     1801 tccggatgaa cttatggatg cagcgagaat agatggtcta agtgagttca gaatatattg
     1861 gaatattgtt cttcctctag tgaagcctgc tttggcaaca ctggccatat ttaactttat
     1921 aggaaactgg aatgccttta tgtggccgct aatagtatcc tcgaactctg aaatgtatac
     1981 tttaccggta ggacttgctt atttttcaag cgaagcttcc ttccagtggg aacagataat
     2041 gaccggtgca acagtttcaa tgattcctct gataatagtt tttctcattt tccagaaaca
     2101 gataataaaa ggcatagccc tctccggatt aaaggggtga aaaaatgaaa atcatagatt
     2161 cacatgttca ttttccggca atagatttta tgggagacgg taaaaagaca catcctgttc
     2221 agcatcatcc gtggctagaa aaggaaagaa acaagtggaa acaggcatgg aattttggtg
     2281 atgaacaacc tgtcaccgat gaaggaaaag ctattgaaga ctggtacttt caggcactta
     2341 agcataatct ggacagagtt gtttttgtaa cggcaggtgg taatatgctt gcacagaaaa
     2401 tagttgaaaa acatcccgac aagtttattg cctacgcaca caacgaccct tcaactaagg
     2461 gtgcagaatc agaactggaa agggctctta agtacggagg tttgaagggt tacaagattt
     2521 tagggcctgc agttgagact ccgctcaacg acaagagtct ttatccggtg tgggaagtgg
     2581 cccaaagtta tgatgttccc gtccttatcc actttggcat tatgggggga gccggcggaa
     2641 ctgcttatca tgtcaacata aatccactta taattcacga tgtagccaaa gcttttccga
     2701 atatgaaaat aataatccct cactttggct gcggatatgt ttttgaaaca ctcaaccttt
     2761 gttgggcatg cccaaatgtt tttatcgaca caagcggatc caaccagtgg atgagatgga
     2821 tgccttatga tgtatcactt gaaagtcttt tcagaaagta cagggagact ataggaccgg
     2881 aaagaataat tttcggaact gattcaagct ggttcccgcg cggatttaca tctgcctatc
     2941 ttgatgaaca gctccgggca atgatataca caggtttttc cgcagacgat atagataaag
     3001 ttctatacgc taatattgcg cagcttctta ggataaggtg atgcgatatg gcttttaatc
     3061 ccatatatac tgaaaaagta cttaatccaa gttataaaaa ctggaaagat ttgtttttca
     3121 gccattcggt ctacctgcac agggctcact tgataatgct tttaaaaacc ggaatcatac
     3181 cccatgaaac agctcgaaag cttaaatatg ccatagacag tgtagcccaa aagaaaaact
     3241 ttccaaagga tatccccccg ggaagtgaag atctttactt tgtttttgaa aaacagatgg
     3301 aagaagttat cggacaggat actgcaggat ttctgcatac tgcacgcagc agaaacgata
     3361 tggataccac ggtttttaga atgtttgtcc gtacacgtct gatggatttt ttatcctgct
     3421 gtataaacct gctttccgta atacaagata aagtgtacaa gcagggggat actcctatag
     3481 tactttatac tcatggacag ccggccaatc cttccaccct aggtcattat ctttcggcat
     3541 tcgcccttga tctttgcgag gatataaatg agcttttttc agctttacgt atagttgacc
     3601 gctcaagtat gggagcatgt gcaataacta caacaggttt taacattgac agaaagatgg
     3661 taagtgatct tttgggattt gaaagtcctg tttccaactc ctaccaagca attgtaacct
     3721 cacactggct tacatacccg gccgatgcgg tcagaaagat tctaaatgat gtaacgcgtt
     3781 tcagtgcaga tctttctcat aaagcctcct gtgaagtcgg aatgcttttg tttcctgatg
     3841 aacttgtcca gataagcagc ataatgccac aaaaaagaaa tcctgtcata attgaacata
     3901 taagaatcca agccggaatg gctatgggca taatgaatat gatcaacgag ctgttccata
     3961 atgctgcata ccaagatatt aacgaagtgg ctgatgctcc tgtaaaagag tttgaaaata
     4021 gtcttgagct tgcagaaagt gtagttgcct taatgtctca gaccataaag aatatagata
     4081 tttcccggca gcgcgtatca gaaatagctc tcagtagtgg aacaaccaca acagagctgg
     4141 cagacgagct tgtgcggtta tatggaataa gtttcagaca ggctcacagc ataactgctg
     4201 gatttgtcag atcaggatac gataaagatg ctctgagaaa tattttccaa gaaaaaactg
     4261 accgttcact aaaactttcc gacaaggaaa tagatgaaat cctgagcata gaaaggttca
     4321 tatctgtacg caaagtatcc gggggacctt cggaagaagg catggaagtg gtacttaaag
     4381 agctcgaaaa atatatttct gcattttcct tcagccttga taaaaaagaa aaagatattt
     4441 tatccgcaca gcaaaatctg aaaactgaat atgatataat ctagtagtgt gtatcactgt
     4501 attgctgatt tttagaaagg cacccttaag gtgcctattt tatatgagga gcagatatga
     4561 tctcaaaaga gctgataaaa aaacttttta ttcaggacct tataaaaacc aataacctca
     4621 tgtcagaaga tgtaaaaaaa tatcttgaat cttacgatgg cagattttca tccatattga
     4681 aagaaaactg tacaatagcc ttggaaaaag gtcttcctct ttgtcaggat acaggaatgg
     4741 ttgaagtttt tgcttatgta ggcataaaag cacgtatcga tgaaccgctg cagaatattc
     4801 tcaatgaagc ggtaaaagaa acttatatca gcaaccctta cagatactca acggttgctg
     4861 atcctctttt tgaccgtaaa aatcaaaaaa acaatactcc ttgtattgtt cacactgagc
     4921 ttacagatgg agattcgatt accttgaagt acatagtaaa gggtggagga agcgagaact
     4981 taagcgctct tttcatgcta agaccaagcg ccggtcctca ggaaataatc tccacagtac
     5041 tgacccacat agagaataac ggggcaagag gatgtcctcc tttaaatatt ggtataggac
     5101 tgggtgggac ttctgataaa gctatggtta tggccaagct ttccctcaca gaaccatttg
     5161 agaaaagaaa tccagatccg cgttatgcag agttggagat gaccatactt tcaaaaataa
     5221 acgatctcaa aatcggttac cagggattag gacaaggtat aagtgcttac tccgtccaca
     5281 taaaagagta tcccacacat attgcgactc tgcctttggc tattgctgca gactgttatc
     5341 tttcaagaaa aggatcttta tgtattggag gcggacagaa tgactagaga tgatatcaaa
     5401 cttctccgta caggccagga acttctttat acaggggaac ttcttattat gagagatgcc
     5461 gcccacaaaa aactttatga gctgtggcag aaaaaagaaa aatttccttt tgacataagc
     5521 aaccatattg tattttatgc cggccccgcc aaaaaagttc ccgaacagcc cataggtgcc
     5581 ataggtccta ctacaagcgc aaggatggac agctttttag agatgctttt tgaaaacgga
     5641 gtcattgcca ctctcggaaa aggaaaaaga agtgctgtta acagacagat gtgccaaaag
     5701 tactcatgcg cctatctggt tactccaagc ggagcagcag cagctctcag tcaaagagta
     5761 caaagcataa gagtggttgc atatgaagaa ctcctgagtg aagctatcca gcttataact
     5821 gtccatgatt ttccgatgat tgtggcgact gatacgaccg gagaagaatt atttatgtaa
     5881 cggatacggt ttttatttaa tttaaagtga tcataaggta taatatgata tatcaggggg
     5941 aaataaagtt gaatattctt tgcataagct gttcaaatgt tgcaggatac agtgaaacag
     6001 gaacttccgc aaatgtatgc cggataatta aaaagcttgt cgaaaagcaa gccggggcgt
     6061 catgcacggt taatatagaa aagcttcatg actataatcc cgctgcatgt aatatgtgta
     6121 tggcctgcgc agggaaaaat gagtgcgttt atgacagcca tttcaacgaa ttattccaaa
     6181 aaatttgcca ggctgatctt gtattctatg taatacctca ctactccatt attcccgcca
     6241 aactgactat agtctttgaa aagatgcagg agatatgtta cgtcaacggt tgccgcgaca
     6301 ggaattatat ctttccgctt attggcaaga gagctgctct tataggccat ggaggaagtc
     6361 ccaaagaaat ggaaccttac tataggactt atattctaaa taccgtagct tcggttctta
     6421 aaagtgtagg cgttaagata gctacctatg gagagcagaa tgataacggc gtagtattcg
     6481 gagtcaccgg ttatataaaa aaagagggga aagatgtacc ggatatgcag atagactgga
     6541 ttcaagtaga agaaatgatc tccggatatg tatcagaaac actttataat attatatgac
     6601 ataagtttaa atcttactgg aggcttcaaa agtatgtttt ttatctatcc tagctttttt
     6661 tcccgcattt tctggcagtt cagaacatga acggaacaaa ctccaaaata ccagagtctt
     6721 ttgacgagct ggagtattta aagcttatat ttgactttaa tccttgtctg atatgtatta
     6781 cagacttgga aacaggtgtt ttcctggatg cgaataagag ttttatgatg aagcttggtt
     6841 atcaaaaaca tgagctttta ggcagttcac cttcaaaaat aaatcttttt aaaaggagat
     6901 cggatcttga tatcctcctt gaacagataa aagaaaaaaa agaagtggaa aattacgaga
     6961 ttcttgttca ggataaacaa ggaaaacctt tgtatatatc actttcatca aaaataattc
     7021 tgttgaacaa acgtccttgt atactgtccg ccggaataga tatgagcgat aaaaaaatta
     7081 tggaggatga gttcaatgaa tctgttttct actcatactc ccttaacatg cttataaacg
     7141 aaatatccac tgagcttatc cagtgccggc acgaagatat agaaagcacc atagatatta
     7201 ctttaaaaaa gataggttct ttcagtaacg ttgacagggt gtatatcttt gagttcagcg
     7261 acggaatgaa gtgtctttca aatacctatg aatggtgcga agatggtata aaaccacaga
     7321 aagagcagct caaaaatatc tcaagcacta ctataaaaag atggatagaa cggtttaacg
     7381 aaaaaaaaga ggtttatatc tactcagtca gtgaaatacc ggatgagtat gctttagaaa
     7441 aggagagcct tgcttctcag gatataaaat ctcttctagt aattcccatg atctacagcg
     7501 gcaagctcat aggctttctc ggctttgatt ctgtaaagga ttacaggttt tgggatcaga
     7561 gcattataaa catgttttct acgattgctg aaatacttgc ggcaaccctc ataaaaagag
     7621 actacgaaaa agagcttata tcagctaggg atatagctca aatggctgat aaaagaaagg
     7681 gagagttcct tgccagcatg agccatgagg tcagaaatcc tgtaagcgtt atagtaggtc
     7741 tgagtgagct tttaaatctc agaataaaag accccaaaga aaaagagtac ataaacggaa
     7801 ttatggttaa cggaagaaac cttcttaaac tgctcaatga tattttggat ctttctaaga
     7861 tagatgcagg caaaacaatt ctacatatac aaaaaataga tctagggtct tttcttggcg
     7921 agtttgaaca gatctacggt atgcagttca agaataaaaa tatatcgttc atacttaaga
     7981 gacatccgga tcttcctcag aatatatgga cagatgaagt aagactgcgc cagatactga
     8041 tgaatataat aggaaattcc gaaaagttta catatgaagg ctatgtagcc cttgaagcaa
     8101 agtttttgaa agtatcgaac gaatatggcg atatgtgttt tgaaatctct gatagcggaa
     8161 ttggaatatc tcaggaaaat attgaaaaac ttttcgaagc tttttcacaa aatcccaatc
     8221 aggacagaaa atatggaggt acgggattgg gcttgagtat ttcaaaaaaa cttgtcgaac
     8281 ttatgggagg acatatctcc gttcaaagca ctcagggagt tggaacaaaa gttagtataa
     8341 cactgcacgg aattaaatat tccgacactg cacggaatta atattccgat aattaaggag
     8401 agcttatgaa caaactcgtt ttcattcaaa ctgtattctt tttatctatg cttcttatct
     8461 cctgcagcgc tgaaaaagat attaaaccat tttcgataac ttccgaaaaa aacgttatcg
     8521 taaaacccat gcctctgaag tatcttctca gcgataccct tgaagagata taccttaccc
     8581 aggtagccac aaatgatatc tacgatagaa aaacctatga atcaattgct aaaaactttc
     8641 ccaaaataaa agaacatttc gaatggatgg aagaaaccta tcttctcttt tcagaggaca
     8701 tgaaacaaga gcttgaactg ctttacggca aaagaagaaa ggttaatcca tggtacctgg
     8761 ttgattttgt cacaaatctc tatgatgaag caagctttga tgaaattatg gcatactatc
     8821 ggtccataag cttctttgga aagtactcct ttatgcaagg tcagacaaag gaatcgatac
     8881 taaaactttt cccttacttc tacaataatt ttttcaggga gtacatagaa aaaaatcaat
     8941 cctactacat tgaacttgcg caggatatga accgtaggat agaagacgat ccttttgata
     9001 ttataactta tatgcagaag atgagcggaa taaacttttc cagaaagtat aaggctgttt
     9061 tttactatgg ttacagacct ataggtgcct ttggctctgt ctacgaggat ttttatattt
     9121 caactattcc acctaccgta aaagagtaca gcactcttta ttcagtaccg tttcatgagt
     9181 tcagccatga acttttcaaa acctttacca gggcaacacc atttactgac gcctgtaaat
     9241 ccaagcttga agataagcaa ttttatgatt actggataaa taaaggactc aacaacagtt
     9301 acggcacatg gtcttcctgg tgtgaagaaa atcttgtgga aggtttttcc aagtaccttt
     9361 actttgtacg gtacggatat atgaaccaac agcctacgta ctactatgac atggagtttt
     9421 atgaatttct taaaaataca gactttcata tccgtctgag caataaagaa accacacttg
     9481 aaagagcctg catagaattt cttaccaaat aacaaaatcc cctttctttt cggaaagggg
     9541 atacttttta ctgataaata atcaaggaag gatcaactgt ggaaggatct gtgaagaaac
     9601 tgccaagacc tttttccaca atttccggat accttggtcc acttacttca agagccctgt
     9661 ccggatttct ccttgagaac tgctttatta cttcaagagc gagagaagca tgctgtccaa
     9721 ctctcttttc catagaccaa cctgtttcat cgtaaggaac ataaagtatt ccgtattttg
     9781 aagcttccaa tatgaatccg tctttacctg tcataaggag ctcattcgtc ttcggacctg
     9841 ttatttggtc aagtccggga ccgggtgatt caaacaggaa aggtaaagta tcggaataat
     9901 cacctatctc tctgtgtgaa aggcctctaa agctggatgg agaaggttca agatggtttt
     9961 caaacttttc ttttgccttt accgacatag aaactccaag agctatctgc cttgacttat
    10021 caggaacaac tatacagttc gttacaggaa acataacttc cgcttcatgc agatctatcg
    10081 caacgtctat tttctcttcc ttcatcatct gcatggccgc ataggttatc ctttctgcaa
    10141 gaaaaccgtt tggtcttcct ggccatgatc tgtttgtgtt tctggcatca atgtatgaaa
    10201 ggagctgttt gtcaggataa tgaacatata cgtcagggtc gggccactgg tcaagctgtt
    10261 gtaatcctct gtcgcccatt ctgtacttct tttctcccca tggagtttta aaggagtagt
    10321 aaagtggaaa tcctccgccg ggctgtgtac ccagtgaacc gctgtagtta aaatgaggta
    10381 tcacatagac cgtacccttc tggacagtaa ggttttccaa gaacagaaac gctgtgagca
    10441 tggcagaaat ttcattttca tgtgtaccgg ccattaaaag gacctttccg ccttcttcct
    10501 gacctttaaa cacaaaaaca tcagtatctg ctatggtacc ctttattcct tcaaagtact
    10561 tgcttaactt aaaaacttcg gtaatgcctg gccctttaac ggcaacttcc ttgaagttcc
    10621 tctggtttaa aagaggaata cctccggcaa ataccatcag tgcaataata ccaaccatta
    10681 taagttttcg taaaatcatt tttatcaacc ctctcacttg aatctgaata atctcagaag
    10741 gaatggcaga atagtaagaa catacatcag ttccttctgc agattctttt ccttgaaaag
    10801 atttttgaag agaaacaccg caagaacagc tacaattata tagtatacga cattgattat
    10861 ttccatcata tcaccccgtt ttaagagaac ataagaaagc taagctgatt gctgaatatt
    10921 atcatcagta atccgacagc cgtaatcgct atccaaggaa caatcgattt tttaaggaat
    10981 gaagagtact ttcctgtata tccggctgta cttacgctta atcttcctat aagtgccgtt
    11041 ggaggcaggg catcacccaa agcccacata aagcttaatc ccgctattgc aagcacagca
    11101 ttgtatcctc tgctgttaag catccatacg agcggtgcac ctataagagc tgcgcttccg
    11161 tatgtaagga ttgcctctga aaccggaata atagccggga gaagtatgaa aagcaaaact
    11221 accggcattg taacaaccgt catggatata agtcccttta ctccattgaa gcttattacc
    11281 tggagcaaca ttcccacaat agctgtagta gctaaaagag gaataacctg atcaatggtt
    11341 gactcaataa ccttaagaat ctttatcctc ttgaaattca tagcaaaagc cacaagagca
    11401 cttattgcaa agttaagagg aagtcccatt acaggaaaat caaaaggcca tatccttggt
    11461 gccgtaagaa ggaatataag aaccaaaaac ggtattccta cactccatcc gctgagtccc
    11521 ttaggctctg gaatattttt caaggcggca tctctgtcta tattcttggc ttttctccct
    11581 aggaaaagaa ccgagaacag accaagaact attactggta tcagaagggg aaacgtaaaa
    11641 ccgacatacg gaatagccgt acatgcacat gtcatcatgg cccatacgtt aataggcgga
    11701 gcaacggcac tcattgctgc ggtaagaaat attatggcag atacggtatc aagtcccaga
    11761 cccatagcca caagcgctga tgacaccgtt cctccgacaa ccataacaga tatgctgccc
    11821 gcaccggtta tagaaccagg aacaagcatt accaccataa ggagaatgag catgagatac
    11881 tttttattgt aaaatccttt tataattcct cttactatgt aatctgtacc tccagactct
    11941 tttatgatat tcataaaaag agttgcaaaa aggaagatta aacctatatc aaagtacgca
    12001 atagaaccct ctgcaagatg tctgaacgta tcaaaccctg caccgccata tatggctcct
    12061 gcaactacgg caaagaatag acttatctct gtcgaaaact taaaaagttt agagacgaca
    12121 taagctcctg ccattactac aagggcaagt atggtctgta tgtaaagaga agaagttgac
    12181 aaattaatcc ctccagactt atattaactt acttggctgt tatgaatatt tcaggaacaa
    12241 gttcaagcaa tgattcctga gaatcaattt caataaactt aagatttttg ctcttggcta
    12301 actgagtaaa ctttccatcc ttattggtat ccttggtggt tatgataacg tcacagaaag
    12361 gtgcaaccgc atcgataatc tgttcgttta ggcttccagg caatcctctt cttatttctc
    12421 cgccgattgc aagggctatg agggtgattt tcttatcttt tgcatagttt ataagctttt
    12481 taagtcttga tacttcatca tcaactgaaa ggcctgatgc acccatacct ttcaagcttg
    12541 caccaagaac aagccacaaa gattcatact tggttccctt agcctgagtc gaggttactg
    12601 taacatgttt tccggggccg ctcttggctc caccaaggcc tactcctgca gcaaactctt
    12661 tttcggaaag aatatcacaa tagtcatact taatacccat ttcatcacat acataagtaa
    12721 ggatctccgc ttcggaactc tgacccgctg aagtaataac tgtaggatac tttaaaaacg
    12781 gtgtttgagc tccaaaggaa acagatgcaa aaacaaggga aagaatcact atggttagag
    12841 ctagtttttt catttttaac ttacctccat atatttattg aaaatcttaa agtgtctgcc
    12901 tctttcgagg cagacagtta attacaactt actttgcagg tttctgatac tgtatcggag
    12961 ctatctctac tgctttgagc caccagtcag gataaccgac tgaagttgct acattaggaa
    13021 tgttaacata accatcctgg tacttaacca taagataatc ggcaagatcc caccactgtt
    13081 tgtatacgga atcggcatta tctgaacagt actgtgtcag gaagcttatt gcctgagcag
    13141 gatccttttt gtaaagctca agtgctgtct gttcaattat cggctgcata ataaactctt
    13201 ttccttcaac ttctctctgt tttgcctgta tatcttttat catatagttg aacttaaggt
    13261 ctgcaaggtt ggaaacgtag ttaaatgccc accatgctgc atttctgtca agttccttct
    13321 gatttccgat ctgatatgac ttgggaagat ctgacattcc gcagtagaaa ggaacaaatg
    13381 ccgtgctgtg cgcgcagtct gctccgtacc aaagtactgc tccgatagga tcgggcaggt
    13441 aatctctcga ctgtgtaaca aatgtatagg aagctctgaa tatcgatata gctctttccc
    13501 aatcaccttt tacgagtttg cttgaaccgc tgtatctgtt gatgtttcca aaaggacctg
    13561 ctgctattcc ctttgaaaga tcaaattcag tatcctggta ccagtctctg tagaggtcta
    13621 ttacatctgc aattgtaact ttcttttccg gttttaccga gaatggatat gcttttgtaa
    13681 agccatcttc aacccatgcg ctgagcttta atgaaggagc aagcctgtcc attattctcc
    13741 atactcttct gagactgtag taaggatgtg tgtactcacc ggtgcttact gttctgagcc
    13801 agtcaaaatc gccttttcca tcccaccagc cggcagtttt ggttatttct ttaaggttag
    13861 gtgagaacat aacgttagga tcattttctg gaagttctct tatcctgaat tcgttagctt
    13921 ctacgaatat ttctccatca ggaactttcc ttgcagccca aagagcactt tttcctgaag
    13981 gatctccaca catttcaaat acccatactt ctttggtatc agctattgta agtgtttcgc
    14041 cccagccgta gtaaccgtat cttactccga gttctcccat taactttata gcttctctgg
    14101 ccgttgtgca tctctcaaga gcaaccattg aaagtgacga aatatcaaag attctttctc
    14161 cggctttcgg ttgtgcatac acctttgcag aacatgtaca ttcacctata gaaagctgat
    14221 gctcgttcat tataccgtat gagcccataa agtacgcata tgtatgcgga acctgttcta
    14281 tgctccctaa agcctctgta agaggatatc ccggaagatc atagttggga ccgaaatctt
    14341 ttccgttata tcttggaaat ggctccacat aaggatacac atttcttctt gaacccggtg
    14401 catgatccat ggcaggaaca tatataagtc tgaagtcgca gtcaccgctg tcgcatgaat
    14461 gggtaacact cgtcgaaccg tcaacggaag cacctttggt tactgcaatg gttgtacatg
    14521 ctattgcaaa agtaatcgat attgcaacca ttaatactgt aagcaataat tttttcatta
    14581 ttctcgctct cctttatttt acgggttttt gatactgtat aggagcaaac tctattgcct
    14641 tgagccacca gtcaggatat cccactgatt ttgctacaga aggaatattt acgtaaccgt
    14701 cctgatactt aaccataagg tagtctgcaa gattccacca ctgtttgtat actgaatcgg
    14761 catttgcaca tgcataatct gtaagatact cttttgcaag ggcaggatcc tttttataaa
    14821 gttcctgagc agctttttca atcattggct gcatagcgaa ttcttttccc tcgacttctt
    14881 tctgtttggc ttgaacatcg gtaagcatgt agctgaactt tatgtccatt aagtttgaaa
    14941 cccagttgaa tgcccaccac gcagcatttc tgtcaacttt tttctgtgtt ccgatctgat
    15001 aagatttcgg aagattattc ataccacagt aaaaaggaac gaatgcactg ctgtgtgcag
    15061 catcagcgcc ataccataat actccgccga ttgcatctgg cagatatgac ctggactgtg
    15121 taacaaatgt atatgaggct ctgaaaacag aaattgctct ttcccagtca ccttttacaa
    15181 acttgcttga accactgtat ctgttgatgt ttccaaaagg acctgccgct aagcctttag
    15241 aaagatcaaa ctcggttccc tgataccaat ctctgtaaag atctattata tctgctatcg
    15301 aaagcttgtt gtctggtttt acagagaatg gatatgcttt tgtaaaacca tcttcaaccc
    15361 atgcactgaa cttctgtgaa ggagctatcc tgtcaaatat tctccatact ctcctgagac
    15421 tgtagtaagg atggctgtac tcacctgtgc ttactgttct taaccagtcc agttctccgt
    15481 ttccatccca ccatccggct ttcttggttg cttcaaaaag atttgaagac cacatcatat
    15541 caggatcatt tacttttatt tctcttatcc tgaactcgtt ggcttcaaca aacacttcgc
    15601 cgtccggaac ctttttggct acccaaagtg cgcttttgcc ttccggatcg ccgcacatct
    15661 caataaccca tgcttcctta gtgtctgcta ctgtaagtgt ttcgccccag ccgtagtaac
    15721 catacttctc agcaagttct cccatgagct taacggcctc tcttgctgtt ttacatcttt
    15781 ccatagcaat cctggaaaga gctgcaatat caaagattct ttctcctacc ttaggctgtg
    15841 catacacctt tgctgaagca gtacactcgc cgatagccag ctggtgctca ttcattatac
    15901 cgtatatacc gtctatatag gcatatgtat gggcaacctg atcaatataa cctaaaggtt
    15961 cggttttcgg aaattcagcc atttcataac cagggccgag atccgatcca acatatcttg
    16021 gatatgcttc gccgaaagga tatattgctc ttttggcacc cttttcatga tccatcgcag
    16081 gaacagagat tatcctgaaa tcatagtctc cgctgtctgc tgaatgggta acgcttacag
    16141 atccgtctac cgatgcaccg gctgaaacag ctatggttgt acacgctatt acagcagctg
    16201 caaatgaaag aagaaccaac aatactgtaa gaagtttttt catgccatac actcccctgc
    16261 aataaatttt agggacatca ctatccctaa taccccaaaa gctttctttg atataaaaca
    16321 cttcaatttt ataattagta gttaatataa ctaactatat ttttatacgt tctcctaaca
    16381 atagaaatta agtatagtca attaatgttt aaaatctctc ataactctac tcagactttt
    16441 aaatgctata aattaatcgt tgttatgaaa aaacaataga taaatctata ttacttaact
    16501 tcttttaaat ataagtaatt tctatacact gttatttata ttacagttgc tgtttgttca
    16561 tattatatct acataaaaaa aaccgagaaa gagctctcgg cagtttaaac ttttattttt
    16621 taaaagatac aataaaatga agattattat ttttatccgt tttcgattta accacaaacc
    16681 attttccttc atactcaaag cttccgattt tatatgcaga gtcttttaaa tcacagggct
    16741 ctgatgaacc ggacatgaca aaaaaccttt gcgactcatt tacatcttgt tcatcaatgg
    16801 agttgcatat accgtcatac actattagct ctgaaagatc ttcattaaaa ccgtttctgc
    16861 acagatccgc ctccaataca gccggaatct ttgaataatc tgtgtatttc acagaaacaa
    16921 gaagctcacc tgacgactcc tccaaaagat actcggcact gaatccgatc tcaggaattt
    16981 gtgaaaaaac atagtagtcg ttgctgaaaa tactgaaggt tttttgatac tctgtcttat
    17041 ttttgctcca aatatccaga acatactcat acagtctctc cgactgagca aacagcaatg
    17101 ctgctgaaaa aatcatgaaa gctgttaaaa aatttttttc atcttatctc cttatctgta
    17161 ctcctcaagt gggtttatac tttcaattat gttttgagcc tttacctttt cctcataaaa
    17221 taattcatcc ggtgtatctg aaaagtcata ctcataaatt tcttcttttg cttgtttttc
    17281 tgtttcttct acaatttctg catctgtatg gactgaatct ttattttctt ttgcttcttt
    17341 attatagcaa tccataaaat caggatataa aatatctatt gttatgcacc tgtacttttc
    17401 tgttataggg ataatctcgt cggcttcaaa accgaaagac tcataactca tcttatcgtg
    17461 cgcacaggct ccaaatggat ctttatcttc atccataatt atctcattct tgcctatgct
    17521 gcctataaca atgttttcat cattaattga ggttatgaca ccataaggct gtatacatgc
    17581 aaagggaaga actttatcca ctacataaag atagccgtat ctgtcaaata ttcctacctt
    17641 atctccaaag atattggtta tatttcccaa attatcaaag ttataaaagt tgttcatagt
    17701 catacacctc gtataataaa aagtagtagt tattaaagtt cctctccaag aactttgag
//
LOCUS       DAOSHR010000020        17466 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_2653_length_17466_cov_5.581644, whole genome shotgun sequence.
ACCESSION   DAOSHR010000020 DAOSHR010000000
VERSION     DAOSHR010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 17466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17466
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2653_length_17466_cov_5.581644"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /gene="pelG"
                     /locus_tag="PLU28_02010"
     CDS             complement(<1..254)
                     /gene="pelG"
                     /locus_tag="PLU28_02010"
                     /inference="COORDINATES: protein motif:HMM:NF028243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide Pel transporter PelG"
                     /protein_id="HOG33872.1"
                     /translation="MAGIGFKLNQIMKNRTIAGDILAFSYSAIVSAGPWIITSFTLWL
                     LLSVLQSSNIYFNSAMIYSSVFSSLLTSGILMVETRLISDL"
     gene            complement(254..1663)
                     /gene="pelF"
                     /locus_tag="PLU28_02015"
     CDS             complement(254..1663)
                     /gene="pelF"
                     /locus_tag="PLU28_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GT4 family glycosyltransferase PelF"
                     /protein_id="HOG33873.1"
                     /translation="MRVGIIFEGTFPYVTGGVSSWANTMMRSMPDIEFTVIHISAKPE
                     KRIPKYKFSPNIKDYFEFSLFAKYKSKKKPLPYSIAKEIAETIVYPFDEKYMSVKLYE
                     ILKKAYNYDFTVIPESDAYWDFLESIYKKFIPYEDFNYFFWNVRSMLLPFMNALTVDL
                     PVCDLYHSVTTGYAGLTALMACLKYNKPFIITEHGIYHRERQLELLRAKWVKDEYRTG
                     WIRLFNTISALTYYGSSAVTTLFLKNQIIEKELCDDHKKLSIIPNGINYELFSSLGKS
                     KEQKTFNLGLVGRVVEIKDIKTAIKAFKLINEYRKDIYFFIIGPTDEEPEYFRECQNL
                     VQMLDLNDYLSFTGSVDVKQWYPKIDLAVLSSVSEGQPLALLEAMACGIPVVATDVGS
                     CSEIIFGTSGDDDVHGACGEVVNSKDFVGLSKAILKFANDPELYKNASQIAKMRVEKR
                     YELKNMLYSYRKIYESVVL"
     gene            complement(1663..2289)
                     /locus_tag="PLU28_02020"
     CDS             complement(1663..2289)
                     /locus_tag="PLU28_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG33874.1"
                     /translation="MNKIINFLLKPGILTLTLMIMVLVLVFFMNYNETVLVTTEELIN
                     LPYEKRSLFIKELEIQSFLEKNPSDINSMFDLRNIYLSVLKNLNDIEKTKLYNEKLLT
                     IDKKILDTYLVSINNFISSSRVDFEAFSSENNTVMNDIFRFSLNSSIMRELYNQLNWY
                     QKGYFLIICKYYNRLDETILSDFILFGRNYVDIDFFNSVMDMVRRGEY"
     gene            complement(2325..3266)
                     /locus_tag="PLU28_02025"
     CDS             complement(2325..3266)
                     /locus_tag="PLU28_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG33875.1"
                     /translation="MIDKRLGYLIFSCITVIVGFVIIGFLLVQSYINALYSYAIYFSL
                     AVVNILFLKLSFSQAFYELVLNMSIFPFYPLIRALGMFSDRPVFRPFKSEEFQVKLSE
                     IQLVDVSDFAPVLIISKYGDTFQKKAGVRLIVELIKEEKIDINQSIQLLKELQKDQHP
                     DVVMYASDAMTELDNYFLQKIEESKDRIDDPDYLKKYGSYADYYIKSGLVSGIIKDNL
                     LKNVISVFEKRLEKLSFRFDIVDVYLTLIETSGRNPLEPAIRFKEILATQPILEKAIL
                     YSIKYNKLEITKKLIKEFLEKGFSPEHPSLKFLLENQ"
     gene            complement(3259..4542)
                     /locus_tag="PLU28_02030"
     CDS             complement(3259..4542)
                     /locus_tag="PLU28_02030"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOG33876.1"
                     /translation="MKDGGVIITKNKGQAAKLLPVFELFGISTFFFIIDLFQGLFGYI
                     HFFPNPYLLITIFTASRHSIVFGLTNSVLNVLYQYISIYTNIVKNNGDLSLIYSFDFA
                     KYPIIYLVTAFVFGLLKEIDTLRIKNAEQQVKDREKIIDDLKEILRRHKDAIQDLKEK
                     LSLENKGISLLVERLREMQISKPEDIYNEGVELISNFIGAENVSIYTLDASGFLRVKV
                     RKGEGILPNSLRPDDSIVLKYALKDGTANINVQIVTDEQGKLPVEPAIASTIKNGDNL
                     IGIIIIEIIDPFKLNKNTETYLKVIGDWFATMLASSQSITHELEILTTNADGTIRIEY
                     YESYFEDYKKRWDRFRMPFSLLKVRTGEVYKEEILKIKRKMDIVFSEKNEQEVVLTFL
                     LGVCDSQGLQKVLERLKNKSGKEFEIITSWSSDND"
     gene            4583..5563
                     /locus_tag="PLU28_02035"
     CDS             4583..5563
                     /locus_tag="PLU28_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOG33877.1"
                     /translation="MYRRLIKIPQQLQPVEILGAYNNRVKYIKEKFDIEINYSNNSII
                     IYGQTDSPVAKVESIFHELIEVLETGHLPDWSEFQYIVSKYESSDESFEPSNLQNSSM
                     DFVNTTISGIKIMPKTQGQAAYVKILREKDMVFSIGPAGTGKTYLAVAIAVEYLRSGK
                     VQRILLTRPAVEAGEKLGFLPGTLYEKIDPYLKPLYDSLIDFIHPDKLIMYRERGIIE
                     VVPLAYMRGRTLNNSFIILDEAQNTTYQQMKMFLTRVGFNSKVVVTGDITQIDLERKK
                     DSGLLAVRNILGNIEQISFMELDKNDVVRNPLIKEIINAYEIFERDNNME"
     gene            5569..6936
                     /locus_tag="PLU28_02040"
     CDS             5569..6936
                     /locus_tag="PLU28_02040"
                     /inference="COORDINATES: protein motif:HMM:NF019318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOG33878.1"
                     /translation="MKKKVTTESNQKGVIVRVLIFIVLSVFTELLLWKKLTFAFQIKF
                     NLIMMAVWIPIIELIMSYKRYFRLHRQNYWGAFMLPLILNIVINLFIFKYLNIFGLTT
                     ILSTLIISMLIDFESGVLSAFVFSTFFGLLFGFDFRYAIIVFIPGVLIAFISQKITRR
                     IEIFFPFLLGSLLQTFLLYIFGYSRNTLDFIVIVAVNFMGILVSMGILPFIEYITRVY
                     SDIGLLELGNLNHPLLRELSMKAPGTYYHSMLVANMLDSSVEYIGGRTVLARIGAYFH
                     DIGKTWKPLFFTENQKDENPHDKLSPKLSSLVLDYHVRYGQELAKKYRLPILIEDMIV
                     QHQGTRVKQYFYKKYMETTGNKNPELFRYPGPVPQFKEAALLMLCDITEAYARTLKDL
                     TPAKLSESIDNFLQSLYFEGQLDECGLTVKELKKIKGTIIRTILAMNHKRINYPKLDE
                     KDIKR"
     gene            6940..7371
                     /gene="ybeY"
                     /locus_tag="PLU28_02045"
     CDS             6940..7371
                     /gene="ybeY"
                     /locus_tag="PLU28_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOG33879.1"
                     /translation="MKINVINEQNIKRVSVKKIRDISKYILSKEFKEGPYSLNILLCD
                     NMRIKELNENYRRKEGPTDVLSFAYGLNEPVIGDIVISVQRIEEQAKDYGNSFEQEFY
                     YNLIHSLLHILGYDHETSEEDKKHMFELQDSYFSLLVIQAE"
     gene            7406..8650
                     /locus_tag="PLU28_02050"
     CDS             7406..8650
                     /locus_tag="PLU28_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HOG33880.1"
                     /translation="MSTVRPFKALRPVKNYAQEFSCPPYDVLEENEVKQIVQEYPNSF
                     LRVTRAEVEFKDGADPHSDVVYKKAAENIKMFEKTGVLIEDEKRCFYLYRETWKGRTQ
                     TGLFATFSIDEYDKGLIKKHELTRQDKEDDRARHIITLEVQAEPIFLTFKSYPEIKEI
                     LSLMIKKYDKLYEVTDSNGVLHEVWKVDSETDVNLIEKSFKKVDCLYIADGHHRAAAS
                     SRARKQLMEKNPSHNGSEEYNFVMGAIFPDDELRILDYNRVVKELNGMDSNEFLKKIE
                     ENFTVSFACEKPYSPKELHEIGMYLDKKWYSLKAKDHIISKTDAVKALDVSILQEYLL
                     CPVLGIENPRKDPNIYFLGGIRGVGALEDWIDEKKWKVAFSMYPTQLSQLIQVADDGM
                     IMPPKSTWFEPKLRSGLTIHKI"
     gene            8710..9231
                     /locus_tag="PLU28_02055"
     CDS             8710..9231
                     /locus_tag="PLU28_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HOG33881.1"
                     /translation="MKSYFFDILKKSERIKTNKNDLEFDFSEYREFNQYTYGFKRSWK
                     ILYAHNDISAVKRIINPVSNLMLSSFKETEKSTLSYMIYIDFGKYESNRKMLTFYDLE
                     LDIIILKDLSYQILDMDELIEAFENQRITQSELNTVLMVLQKTVNSFNSRGIFKTLED
                     MYGQASIQWLLEA"
     gene            complement(9198..12050)
                     /gene="uvrA"
                     /locus_tag="PLU28_02060"
     CDS             complement(9198..12050)
                     /gene="uvrA"
                     /locus_tag="PLU28_02060"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOG33882.1"
                     /translation="MEWIKVRGACEHNLKNIDVDIPKNKLVVFTGLSGSGKSTLALDT
                     IYAEGQRRYLESVSTYARQFLGDLKKPDVESIVGLSPAIAIEQKSVGHNPRSTVGTIT
                     EIYDYLRVLYARLGKAYCPNCSTPLESSTVDEIIEKIYKNFDEQARLFIFSPIAREKK
                     GEFKKEIEAMKLSGFKMAEIDGKLIELEEVQSLPKTVRHNINLLIDRLKLKKENFQRL
                     YEAIELALREGDGFVTVINTDDNNKYLKTLTFSEVLACPNCGFSFPEITPKLFSFNSP
                     YGACPECHGLGFKMEIDPAYILDVNKTLSEGAVAAMGTDTYMVDSIRQMITFFGADPE
                     KKIKDLPPEIITYILYGTDQPISFKYENSNGSHNFKRSFEGIINWFERRYRETESQDV
                     REWIENNIMVQKTCQSCEGKRLRKEALSVLIGGKSIYELTDLPMGSALEFIEKLELTD
                     FEKQIGSELLGEVRKRIGFLVDVGLNYISLNRAANTLSGGESQRVRLATQIGSGLTGV
                     TYVLDEPTIGLHQRDNDRLIETLRKLRDLGNTVIVVEHDEDVIRAADYVVDLGPNAGI
                     NGGRLVYQGSVDGLIKNPHDSLTGRYLAKIEKIEIFNKKRVEKNRYIKLTGAKHNNLK
                     NVDLEVPLGKFVVVTGVSGSGKSSLIMDTLFPILQNHLYRSKNKPGEYRTIEGLDCVD
                     NVISIDQSPIGRTPRSNPATYIGVFDLIRDLFASSPEAKMRGYDKSRFSFNVKGGRCE
                     ACAGHGVIKIEMQFLQDVYVTCDVCKGTRYNKETLRVMYKGKSIAEVLEMSVDEGADF
                     FKNVVSINRSIQMLKDVGLGYIKLGQPATTLSGGEAQRIKLTAELKKRDTGNTVYILD
                     EPTTGLHFEDVKKLIGVLNLLTEKGNTVVVIEHNLDVIKNADYIIDLGPEGGENGGMI
                     VAQGSVEDIIKSNSYTGQYLKKIFQVQASKSHCIEA"
     gene            complement(12205..12630)
                     /locus_tag="PLU28_02065"
     CDS             complement(12205..12630)
                     /locus_tag="PLU28_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HOG33883.1"
                     /translation="MSLNEIYSDIIIDYSRNPKFKRVLNNSEHSHSKNLSCGDEIDIY
                     IKIEDSIIKDISYEGKGCIISSSSAAMMCETLKGRRYDEALKIIENVLKMSHKEPYDE
                     ELIEDIQVFSDISKYPMRVKCFTLSWHTADDILRSWKKV"
     gene            complement(12627..13838)
                     /locus_tag="PLU28_02070"
     CDS             complement(12627..13838)
                     /locus_tag="PLU28_02070"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufS family cysteine desulfurase"
                     /protein_id="HOG33884.1"
                     /translation="MSIQSFRSYFPALDSQKLIYLDSAATTLKPKIVIDSQTEYYTRY
                     NANVHRSSYALAQQSTQLYEQAREKVAKYVNAKTKEIIFTKGATESINLLAYSLFTSG
                     VIKKEDNIITTGVEHHANFVPWQQISKYNKNEIRYYLPKNGIFKAEELSMIADSGTKI
                     VTFTAQSNVTGQITNTLESINRLKSINPKIMVHLDISQYVSHEQIDVKSMNADFVSFS
                     GHKMLAPMGIGVLWGKEELLENINPFLFGGEMIDYVGIKDSTFNILPYKFEAGTPNVS
                     AAVGLSKAIDFINETGMENISEQINKVTEYAVEKMKNIEFIKLYLPGQEAHSSIISFN
                     IDGVHPHDVAHIMDEMSGIAIRSGHHCAQPLMELIGAQSVCRTSFYAYNTFEEVDTFI
                     DTIYKVKRWLE"
     gene            complement(13819..15015)
                     /locus_tag="PLU28_02075"
     CDS             complement(13819..15015)
                     /locus_tag="PLU28_02075"
                     /inference="COORDINATES: protein motif:HMM:NF013614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HOG33885.1"
                     /translation="MNTLYAKTVRSGHKNFKSVIHEPKRVFESYSEEEVFKKIVKESK
                     NTLLSGYQQKRFDEYKEIGFPLFRRINLNRPDFKAGNFGKPCGSNTISDIDEQGIELI
                     KKYDFEGTDRKYLLMAELFSNCGYYIKTQDDQDAGIIKLWYDDTSIFENSLFSLKPGS
                     KAVIIRQISGNYSDKLRNGVIRAIIERNAELVLINIIKTAKADSISESGYFSLSENSR
                     LKVIELSAGQGKFVSNYVYSMDGKNSVLRSAPMFMGSDESVSDIFHTVKFNAPAAIGV
                     LEAKGVLKDRSSCIFRGTLDINKGAFDADGKESSFTLSLSPHARMQTIPGLLVAENKV
                     NASHAATLGSISEDKLYYMMNRGFSKAQAKKLISMGIFDELTEALDSYDKDIAQETVD
                     YVYSIL"
     gene            complement(15025..16437)
                     /gene="sufB"
                     /locus_tag="PLU28_02080"
     CDS             complement(15025..16437)
                     /gene="sufB"
                     /locus_tag="PLU28_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HOG33886.1"
                     /translation="MSDIQGMIYDLQKGQEKYDFRNKQAAKSMVIPGLNESIIREISE
                     SKREPQWMLEQRINAFKIYETWHEPNFGVDLSDVDLSKIVTYIHPRYKKATSWDDVPL
                     EIKDTFEKLGIPQAERMALSGVGAQYDSEVVYHNIKTELESQGIIFLDTESAVRNYPD
                     LVKKYFMKAFSPSNHRYSALNNAIWSGGTFLYIPPNVDVKLPLQAYFRMNNEGMGQFE
                     HSIIIADEGSRLHFVEGCSAPYYNAINFHAGMVEIFVKKGARVKYSTIQNWSKNTYNL
                     NSKRAIVDQDGVMEWVTGSMGSHKSMTYPTSILRGKGAKNETIAVSFAGPNQHLDTGS
                     KVFMFAPYTSSTVDSRSISLGGGWSFYRGWLKVDERAKGAKATVRCNALMLDNESRSD
                     TIPLIEVFTDDADVGHEAKVGRISDEQIFYLMSRGMSEKEAKSIIVRGFIEPFAKELP
                     LEYAIELNRLLNLEVESTIG"
     gene            complement(16430..17191)
                     /gene="sufC"
                     /locus_tag="PLU28_02085"
     CDS             complement(16430..17191)
                     /gene="sufC"
                     /locus_tag="PLU28_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HOG33887.1"
                     /translation="MSKILEIFDLRASIKEDGREILKGINLSISEGETHAVMGPNGSG
                     KSTLANVIMGNPKYEITNGEILFMGRSIKELSTDERAKLGLFMSFQMPEEIDGVKMRQ
                     FIINSYRNVKTEDEITVLKLSSRISDYAKDLTLSDEFLQRYTNVGFSGGEKKKGEILQ
                     MGILSPRLAILDEIDSGLDIDALKVVAQSITKIKNEKTAVLLITHYQRILNYVIPDFV
                     HVYIDGQIVTTSGASLAKEIEEKGYNFVKAGDAHE"
BASE COUNT         5407 a         3383 c         2831 g         5845 t
ORIGIN      
        1 agatctgaaa taagacgtgt ctcgaccata agaataccgc ttgtaagcaa gctggaaaaa
       61 accgaagaat atatcattgc cgaattaaaa taaatattgc ttgactgaag aacagaaaga
      121 agtagccata aagtaaagga tgtaattatc caaggaccag ccgaaacaat ggcagaataa
      181 gagaatgcga gtatatctcc tgctatggtt ctgttcttca ttatctgatt tagtttaaat
      241 cctattccgg ccattatagc accaccgatt cataaatctt ccggtaagaa tacagcatat
      301 ttttcaattc atacctcttt tcaactctca tcttagcaat ctgtgaggca tttttatata
      361 attcagggtc attggcaaat tttaatatgg cttttgaaag tcctacaaaa tccttagaat
      421 ttaccacttc cccgcaagct ccatggacat cgtcatcacc tgaagttcca aatattattt
      481 ccgagcacga tccaacatcc gttgccacaa ccggtatacc acatgccatt gcttcaagaa
      541 gtgcaagcgg ttggccttca ctcacactgg aaagaaccgc cagatcgatt tttggatacc
      601 attgttttac atcgaccgaa ccagtgaagc ttagataatc attcaaatca agcatttgca
      661 caagattttg acattctctg aagtattccg gttcctcgtc cgtgggacca ataataaaaa
      721 aatatatatc ttttctatat tcatttataa gtttaaacgc ctttattgca gtctttatat
      781 cttttatttc gactactcta ccgacaagcc caagattaaa agttttttgc tctttgcttt
      841 tgccaagact gctgaaaagt tcataattaa taccgttagg aattatacta agcttcttat
      901 gatcatcaca taactctttt tctataatct gatttttaag aaaaagagta gtcactgcag
      961 atgaaccata atatgtaagg gcgcttatgg tattaaaaag tcttatccat ccggttcggt
     1021 actcatcctt tacccatttg gccctgagca gttcaagctg tctttctcta tgatatattc
     1081 cgtgttctgt aattataaaa ggcttgttgt acttgagaca tgccataagt gctgtaagtc
     1141 ctgcatatcc ggttgttact gaatgataaa gatcacagac aggaagatcc actgtaagtg
     1201 cattcataaa cggcagaagc atcgatctga cattccagaa aaaataatta aagtcttcat
     1261 atggaataaa cttcttatag atgctttcca aaaagtccca gtaagcatca ctttccggaa
     1321 taactgtaaa atcataattg tatgctttct tcaggatttc ataaagcttt actgacatat
     1381 acttctcatc aaaaggatat actattgtct cagctatttc cttcgcaatt gaatacggaa
     1441 gaggtttctt tttgctttta tacttagcaa aaagagaaaa ttcaaagtaa tcttttatat
     1501 taggagaaaa cttgtattta ggaattcttt tttcaggctt ggcagatatg tgaataactg
     1561 taaactcaat atcaggcata ctcctcatca tagtatttgc ccatgaagaa acacctccgg
     1621 taacatacgg aaaagttcct tcaaaaatta taccaactct catcaatact caccacgcct
     1681 taccatatcc atgaccgaat taaaaaagtc aatatcaaca taattacggc caaaaagtat
     1741 aaaatccgac agaatagtct catcaagtct gttgtagtac ttacatataa tgagaaaata
     1801 tcctttctga taccagttca gttggttgta aagctccctc attatgctgc tgttaagtga
     1861 aaatctgaaa atatcgttca ttactgtatt attttcggaa gaaaatgctt caaagtccac
     1921 ccttgaagat gatataaaat tatttatact aactagatac gtatcaagaa tttttttatc
     1981 aatagtaaga agtttttcgt tatagagctt ggttttttct atatcattaa gatttttcag
     2041 tacagaaaga tatatatttc tcagatcaaa cattgaattg atatctgaag gatttttttc
     2101 aagaaaactc tgtatttcaa gctcctttat aaaaagactt cttttctcat agggaagatt
     2161 aattaactct tctgttgtaa ctaaaaccgt ttcattgtag ttcataaaaa aaaccaaaac
     2221 aagaaccata atcataagag tgagtgtcaa tatccctggt ttcaataaaa agttaattat
     2281 cttattcata aaaggtcact ccagcactaa ataaattatt ttcattattg attctccaat
     2341 aaaaacttca gagaaggatg ctctggagaa aatcctttct ctaaaaactc ttttatgagt
     2401 tttttggtta tttcaagctt gttatatttt attgagtata atattgcttt ttcaagtatc
     2461 ggctgtgtgg ctagtatctc tttaaatctt atagccggtt caaggggatt gcgtcccgaa
     2521 gtttctataa gagtcagata tacatctact atatcaaacc ggaaagaaag cttttcgagt
     2581 cttttttcaa aaactgaaat gacatttttc aaaagattat cttttattat tcctgatact
     2641 aagcctgatt ttatatagta atcagcgtac gaaccatatt ttttaagata atccggatcg
     2701 tctatcctat ccttggattc ctcgatcttc tgtaaaaaat aattgtccag ttcagtcatt
     2761 gcatctgaag catacataac cacatctgga tgctgatctt tctggagctc cttgagaagc
     2821 tgtatgcttt ggtttatatc aattttttct tcttttataa gttcaactat aagtcttact
     2881 cctgcttttt tttgaaaggt atctccatac tttgatatta ttaacaccgg agcaaaatct
     2941 gaaacatcaa ccagttgtat ctcagaaagc ttaacctgaa attcttcact cttgaaagga
     3001 cgaaataccg gacgatcaga aaacatacct aaggctctta taagaggata gaaaggaaaa
     3061 attgacatat tcagaaccaa ctcataaaaa gcttgagaaa aagaaagctt aagaaaaagt
     3121 atatttacaa cagcaagact gaaataaatg gcataagaat aaagtgcatt gatatagctt
     3181 tgcacaagaa ggaaccctat aataacaaaa cctactataa cagtgataca agaaaaaata
     3241 aggtaaccta atctcttatc aatcattatc gctagaccag cttgttatta tttcaaactc
     3301 tttaccagat ttgtttttta gacgttcgag caccttctgt aatccttgag agtcacatac
     3361 tcccagaaga aatgtaagaa ctacttcctg ttcgtttttt tcagaaaata ctatatccat
     3421 ctttcttttt attttaagaa tttcttcctt gtacacttcg ccggttctca cttttaagag
     3481 cgaaaaaggc atccggaatc tatcccaacg cttcttgtaa tcttcaaagt aactttcata
     3541 atactctatt cttattgtcc cgtcggcatt ggttgtaagt atctcaagtt catgagttat
     3601 gctctgtgat gaagcaagca tggtggcaaa ccaatctcct atgactttaa ggtaagtctc
     3661 ggtattttta ttaagcttaa acggatctat tatctcaatg attattatac ctataagatt
     3721 gtctccgttc ttgatggtcg aagcaatagc cggctccaca ggaagcttcc cctgctcgtc
     3781 agtcacaatc tgaacattaa tgttggccgt tccatccttc aaggcatatt tgaggactat
     3841 agaatcgtca ggccgcaatg aattgggaag gatgccctct ccttttctta ctttaaccct
     3901 aagaaatccg cttgcgtcaa gagtatatat agatacattt tctgccccaa taaagttgga
     3961 tataagctct acaccctcat tatatatatc ttcgggctta gatatctgca tttctctgag
     4021 cctttcaaca agaagagaaa ttcctttgtt ttcaagtgaa agcttttctt tcaaatcctg
     4081 aattgcatct ttatgtctgc gaagtatttc tttaagatca tctattattt tttctctgtc
     4141 ctttacctgt tgttcagcat tttttattct caaagtatct atttccttaa gcaacccgaa
     4201 aacaaaagca gtaacaagat atattatggg atactttgca aagtcgaatg agtatatcaa
     4261 agaaagatca ccgttattct tcacgatgtt tgtataaatg gaaatatatt ggtataatac
     4321 atttagtacg gaatttgtaa gaccaaatac tatgctgtgt ctcgaagctg taaaaatagt
     4381 tataagaaga tagggattcg gaaaaaagtg tatatatccg aacagtccct gaaaaagatc
     4441 tattataaaa aaaaacgttg atataccgaa aagttcaaat acaggtaaaa gttttgctgc
     4501 ttggcctttg ttttttgtga taataacacc tccgtctttc ataattatat tttaacacat
     4561 tttattaagg gagtgatttt atttgtacag acgtcttata aaaatacccc agcagcttca
     4621 gccagttgag atcttaggtg catataacaa tagggtaaag tatataaaag aaaaatttga
     4681 tatagaaata aattactcta acaattctat tattatatat ggacagactg acagtcctgt
     4741 ggcaaaggtt gaaagtatct tccatgagct catagaagtt cttgaaactg gacatcttcc
     4801 agattggagc gagtttcagt acatagtctc aaaatatgaa tcatcagacg aatctttcga
     4861 accatcaaat cttcaaaatt catccatgga ttttgtaaac accacaatat ctggcatcaa
     4921 gataatgcca aaaacccaag gacaagctgc ttatgtcaaa atactccgtg aaaaagatat
     4981 ggtcttttcc ataggtcctg ccggaacagg aaaaacctac ttggctgtag ctatcgccgt
     5041 tgaatatctc agaagcggaa aagtccaacg tatacttctt acacgtcctg ccgtagaggc
     5101 tggtgaaaag cttggttttc ttccgggaac tttgtatgaa aagattgacc cgtaccttaa
     5161 accgctttat gattctctca ttgattttat acatcctgac aagctgataa tgtacagaga
     5221 aagaggtata attgaggttg tacctttggc atatatgcgc ggaagaactc tcaataattc
     5281 cttcataatt cttgatgaag cccagaatac aacatatcag cagatgaaaa tgtttcttac
     5341 ccgcgtaggt ttcaattcaa aagtagtggt aacgggtgat atcacccaga ttgatttgga
     5401 aagaaagaag gattcaggtc ttttggccgt aagaaatatt cttggaaata tagaacaaat
     5461 ttcttttatg gaactggaca aaaatgatgt agtacgtaat ccccttataa aagagatcat
     5521 taatgcgtat gaaatatttg aacgtgacaa taatatggag tgatagcctt gaaaaaaaaa
     5581 gtaacaactg aaagtaacca gaaaggagtt attgtaaggg ttttaatatt tatcgtttta
     5641 tctgtattta ccgaactttt attatggaag aaattgactt ttgcttttca gatcaaattc
     5701 aacctgataa tgatggcggt atggattcct attatagaac ttattatgag ctataaaaga
     5761 tatttcagac tgcaccggca aaattactgg ggagctttta tgttgccgct tatactcaac
     5821 atagtcataa atctttttat tttcaaatac cttaatatat ttggacttac aaccatacta
     5881 agcacactta taatttcaat gcttattgat tttgaatccg gtgttctttc agcctttgtc
     5941 ttttcaactt ttttcggtct tctttttggt tttgatttca gatacgctat aatagtattt
     6001 attccgggag ttcttattgc atttatctcc cagaaaataa ccagaaggat tgagattttt
     6061 ttccccttct tattgggttc acttcttcag acttttctcc tttatatctt cggttattcc
     6121 agaaatactc ttgattttat agttattgtg gcagtaaatt ttatgggtat tcttgtttca
     6181 atgggaatac ttccttttat tgagtatatt acccgtgttt actctgatat aggtcttttg
     6241 gaacttggaa atcttaatca tcctctctta agagaacttt caatgaaagc tcccggaact
     6301 tattatcact ctatgcttgt agccaatatg cttgattcat cagttgagta tataggcggc
     6361 aggactgttc tagcaagaat aggggcatat tttcatgata ttgggaaaac atggaaacct
     6421 ctttttttca cagaaaacca gaaagatgaa aatccacacg acaaactctc accaaagtta
     6481 agttcattgg tactggatta tcatgttcgc tacggtcagg agctggcgaa aaaataccgt
     6541 ctccccatac taatagaaga catgatagtt cagcatcagg gtaccagagt aaaacagtac
     6601 ttctataaaa aatacatgga aacaaccgga aataaaaatc ctgagctttt tcgttatcca
     6661 ggaccggtac cacagttcaa agaggctgct ttattaatgc tttgcgatat aacagaagct
     6721 tacgccagaa cccttaaaga ccttacccca gcaaagcttt ccgaaagcat agataatttt
     6781 ttacagtctt tatactttga agggcagctt gacgagtgtg gccttaccgt taaggaactc
     6841 aaaaaaataa aaggaactat aataagaacc attcttgcta tgaaccacaa aaggattaat
     6901 tatccaaagc ttgacgaaaa agacataaag aggtgaaaaa tgaaaattaa tgtcattaac
     6961 gaacagaaca taaaaagagt ttccgttaag aaaatcagag atatttccaa gtatattctt
     7021 tcaaaggagt ttaaggaagg gccatactcc cttaatattt tattatgcga taacatgagg
     7081 ataaaagaat taaatgaaaa ttatcgccgc aaagaaggcc cgacagatgt tctttctttt
     7141 gcatacggct tgaatgaacc ggtaattggt gatatagtaa tatctgtcca gagaattgaa
     7201 gaacaggcaa aagattacgg aaacagcttt gaacaggaat tttattataa tcttatccac
     7261 agcctgcttc atatacttgg ctatgatcat gaaacgtccg aagaagataa gaaacatatg
     7321 tttgaacttc aagattcata cttttcgtta cttgttatcc aagccgaata aacattattt
     7381 tagcatactt taggagggat ttattgtgtc taccgtaaga cctttcaagg ctttgagacc
     7441 agtgaagaat tacgcacagg agttctcttg tccgccttat gatgtactgg aggaaaatga
     7501 agtaaaacaa atagttcagg aatatcccaa cagctttcta agagtgacaa gggctgaagt
     7561 ggagttcaaa gatggagccg atcctcactc ggatgttgta tataagaaag ccgctgaaaa
     7621 tattaagatg tttgaaaaga ctggtgttct tattgaagat gagaaaagat gtttctatct
     7681 ttacagggaa acatggaaag gaagaaccca gacaggacta tttgcaacat tttccattga
     7741 tgaatacgac aagggtctga ttaaaaaaca tgaacttacc agacaggata aagaagacga
     7801 cagagccaga cacattatta cacttgaagt acaggctgaa cctatctttc ttactttcaa
     7861 gtcttatcct gaaataaaag aaattcttag cctgatgata aaaaaatatg ataaacttta
     7921 tgaagtaacg gattctaacg gagttcttca tgaagtatgg aaagtcgatt ccgagacaga
     7981 tgtaaacctt atagaaaaat cttttaaaaa ggtggattgt ctctatattg cagacggaca
     8041 ccacagagca gccgcttcat ccagagcaag aaaacagctg atggagaaaa atccttccca
     8101 taacggaagt gaggaataca actttgtaat gggagccata tttccggatg atgagcttag
     8161 aatacttgac tacaacaggg ttgtaaaaga attgaacggt atggattcga atgagttcct
     8221 taaaaaaata gaagaaaatt ttaccgtttc tttcgcttgc gaaaaacctt actcccctaa
     8281 agaactgcac gagatcggta tgtatctgga taaaaaatgg tactctttaa aggcaaagga
     8341 tcatataata agcaaaaccg atgcagttaa ggccttggat gtttccatcc tacaggaata
     8401 tcttctctgt ccggttcttg gaatagaaaa ccctagaaaa gatcctaaca tctacttcct
     8461 tggaggtata cgtggagtag gagcgctcga agactggatt gatgagaaaa aatggaaggt
     8521 agctttctca atgtatccaa cacagttatc acaacttatt caggttgctg acgacggaat
     8581 gataatgcct cccaagtcca cttggtttga gcctaagctt agatcaggtc ttacaataca
     8641 taaaatatag tataatatta aggtgatgat tattcatcac cttaatattt attttttggg
     8701 ggatcaacta tgaaaagtta tttttttgac attttaaaga aaagcgaacg tattaaaact
     8761 aataaaaatg atctcgaatt tgatttttct gagtacagag agtttaatca atatacctat
     8821 ggatttaaga gatcctggaa gattctctat gctcacaacg atatttctgc cgtaaaaaga
     8881 attataaatc ctgtaagcaa ccttatgcta tcttcattca aagagactga aaaaagcact
     8941 ctcagctata tgatatatat agattttggc aagtatgaat ctaacagaaa aatgctgaca
     9001 ttttatgatt tagaacttga tataataatt ttaaaggatt tatcttacca gattcttgat
     9061 atggacgaac ttattgaggc atttgaaaac caaagaatca cacaatctga gttaaataca
     9121 gtgcttatgg ttctgcagaa aacagtaaac tctttcaatt caagaggaat ttttaaaacc
     9181 cttgaagata tgtatggtca ggcttcaata caatggcttt tggaagcttg aacctggaaa
     9241 atctttttta aatactgtcc agtatacgaa ttgcttttta ttatatcctc gacagaacct
     9301 tgagcaacaa tcattcctcc gttttctccg ccctcaggtc cgagatcaat tatatagtcg
     9361 gcatttttta taacatcaag attgtgctct ataactacaa ctgtgttccc tttctcggta
     9421 agaaggttaa ggactcctat aagcttcttt acatcctcaa aatgaagacc agttgttggt
     9481 tcgtcaagaa tatacactgt gtttcctgta tcccttttct taagttcagc tgtgagtttt
     9541 attctttgag cttctcctcc tgaaagagtt gtagcaggtt gtcctagctt gatataaccc
     9601 agtcctacat ctttgagcat ttgaatggat ctgttaatag aaacaacgtt cttaaagaaa
     9661 tcggctcctt catcaacact catttcaaga acttcagcaa tgctcttgcc tttatacatt
     9721 accctaagag tctctttatt atacctggtg cctttacaga catcacaggt aacgtacaca
     9781 tcctgaagaa actgcatctc aatttttatc acaccatgtc ctgcacaagc ctcacatctt
     9841 cctcccttaa cattgaagct gaagcggctt ttatcgtatc ccctcatctt tgcttctggc
     9901 gaggatgcga aaagatctct gataagatcg aaaaccccta tataggttgc cggattgctt
     9961 ctgggagtcc tacctatagg ggactggtct attgatataa cgttgtctac acaatccagt
    10021 ccttcaattg ttctgtactc tccgggttta ttcttggaac ggtacagatg attctgaagt
    10081 atcggaaaaa gtgtatccat gataagtgag gactttcctg agccagatac tccagttaca
    10141 actacgaact ttccaagagg aacttcaaga tcgacatttt tcaagttgtt atgtttagct
    10201 ccagtaagtt taatatacct atttttttca actctctttt tattaaaaat ttctatcttt
    10261 tcaatttttg caagatatct tcccgttaac gaatcgtggg gattttttat tagtccatca
    10321 acactcccct gatagacaag ccgtcctccg ttaattccag cattaggccc aagatccact
    10381 acataatcag cagctcttat gacatcctcg tcatgctcta caacaataac tgtattacct
    10441 agatccctta gctttcgtaa ggtttctatg agcctgtcat tatccctctg atgtagtcct
    10501 attgttggtt catcaagaac ataagttact cctgtaagtc ctgaccctat ctgtgttgca
    10561 agtcttactc tctgagattc tcctcctgaa agtgtattgg ctgcacggtt caaagatata
    10621 taatttaatc ctacatcaac tagaaagcct attcttttgc gcacttctcc aagaagctcg
    10681 cttccgatct gcttttcaaa atcagtaagt tcaagttttt ctataaattc aagagcactt
    10741 cccataggaa ggtcagtaag ctcatagata cttttcccac ctatgagaac actcaatgct
    10801 tctttacgta atcgttttcc ttcacaagac tgacatgtct tctgaaccat tatattattt
    10861 tcgatccatt cccgtacatc ttgagattct gtttctctgt atcttctttc aaaccagttt
    10921 ataatacctt caaagcttct tttaaagtta tgcgaaccat tactgttctc atacttaaaa
    10981 cttataggct ggtcggtacc gtacaaaata tacgttatta tctccggcgg aagatccttt
    11041 atcttttttt caggatcagc accaaaaaac gttatcatct gtcttatgga atcaaccata
    11101 tatgtgtctg ttcccatagc tgcaactgca ccttctgaaa gtgttttatt aacatccaga
    11161 atatacgcag gatctatttc catcttgaac cccagcccat gacattcagg acatgcacca
    11221 taaggactgt tgaaggaaaa gagcttggga gtaatctctg gaaagctgaa tccacagttt
    11281 ggacaagcaa gaacttcgct aaatgtaagc gtttttaagt atttattgtt atcgtccgta
    11341 tttataacag ttacaaaacc atctccttca cgcagagcaa gttctatagc ttcataaaga
    11401 cgctgaaaat tctctttttt cagtttaagc ctgtcaataa gaagatttat attatgtctt
    11461 accgttttag gaagtgactg tacttcctcc agttcaatta gtttgccgtc tatttcagcc
    11521 attttaaacc ccgaaagctt catagcctct atttcttttt tgaactcacc tttcttttct
    11581 cgtgcaatag gagaaaatat gaaaagtctt gcctgttcat caaaattttt gtaaattttt
    11641 tcaattatct catcaacagt cgaactctca agcggggttg agcaatttgg acagtacgct
    11701 ttacctaatc ttgcatacag taccctgaga taatcatata tttcggttat ggttccgaca
    11761 gttgatctgg gattatgtcc cacagatttt tgttctatgg caatggctgg agatagtcca
    11821 actattgatt caacatcagg tttttttaga tccccaagaa attgtcttgc atatgttgaa
    11881 actgactcca aatagcgtct ctgaccttct gcataaattg tatcaagtgc cagagtagat
    11941 tttccagagc ctgagagacc tgtaaacaca acaagtttat tcttgggtat atcaacatct
    12001 atatttttga gattatgctc acaagcacct ctcactttga tccattccat aacttcctcc
    12061 tctattatgg tatacataaa tatatagtta ttataataca aaaaaaaact tttttatgaa
    12121 gacctgcaaa aattaacata cattttttat tatcataaaa cttactccgg tcttttttca
    12181 agaccggagt aagtttttga ttgattatac ttttttccac gaacgcagta tatcatcagc
    12241 ggtatgccag cttaatgtaa aacatttcac cctcatagga tatttggaaa tatctgaaaa
    12301 aacctgaata tcttctatta gttcttcatc atatggttct ttatgagaca tcttcaaaac
    12361 attttcaatt atttttaatg cttcgtcgta tcttctgccc ttaagagttt cacacatcat
    12421 tgcggcggaa gaactgctta ttatacagcc ttttccctca taagatatat cttttattat
    12481 tgaatcttca atttttatat atatatctat ctcatctcca catgaaagat ttttggaatg
    12541 actatgttcg gagttattta agactctttt aaatttggga tttctggaat aatctattat
    12601 gatatcactg taaatttcat tcaagctcat tccaaccacc tcttaacctt atagatagta
    12661 tcaataaatg tgtctacttc ttcaaacgta ttgtatgcat agaaactggt tcgacatacc
    12721 gactgtgccc caatgagttc cataagaggc tgtgcacaat gatgaccgct ccttatagca
    12781 ataccagaca tctcatccat tatatgtgcg acatcatgag gatgcacacc gtcaatatta
    12841 aacgaaatta tcgaactgtg agcttcttgt ccaggaagat aaagctttat gaactctatg
    12901 ttcttcattt tttctactgc atactcagtt actttattta tttgctcaga tatattttcc
    12961 atccctgttt cgtttataaa atctatcgcc tttgaaaggc ctactgctgc cgaaacattt
    13021 ggtgtgcctg cttcaaattt gtaaggcaaa atgttgaaag tactgtcttt tataccgaca
    13081 tagtcaatca tttcacctcc aaaaagaaac ggatttatgt tttcaagaag ttcttctttt
    13141 ccccaaagaa ctccgatgcc cataggcgca agcatcttat gacctgaaaa acttacaaaa
    13201 tccgcattca tgctttttac atctatctgt tcatgagata cgtactgtga aatgtcaaga
    13261 tgaaccatta ttttaggatt tatacttttc agtctgttaa tgctttccaa agtatttgta
    13321 atctgtcctg taacgtttga ctgtgctgta aaggttacta tttttgtacc tgagtctgct
    13381 atcatgctca gttcttcggc tttgaatatc ccatttttag gaagataata tctaatctca
    13441 tttttattgt actttgatat ctgctgccaa ggcacaaagt tagcatgatg ttcaactcct
    13501 gttgtaataa tattatcttc tttttttatg actcctgatg taaaaagaga gtatgccaaa
    13561 aggtttatcg attcagtagc tcctttggta aatattatct cttttgtttt tgcatttaca
    13621 tactttgcca ccttttctct tgcctgctca taaagttgcg tggattgttg cgcaagagca
    13681 tatgaagatc tatggacatt ggcattatat ctggtataat actctgtctg tgaatcaata
    13741 acaattttag gcttaagtgt ggtagcagca ctgtccaaat atataagctt ttgtgagtct
    13801 aaggcaggaa aataacttct aaaggattga atagacataa tcaaccgtct cctgtgcaat
    13861 atctttatcg tatgagtcca aagcttctgt cagttcatca aatataccca tggaaataag
    13921 cttttttgcc tgagctttcg agaaacctct gttcatcata taataaagtt tatcctcact
    13981 tatgctcccc agagttgcag catgagaagc atttactttg ttttcagcaa ccaaaagacc
    14041 aggaattgtc tgcattctgg catgtggcga caaagaaagt gtaaaacttg attctttccc
    14101 atctgcatca aaggcacctt tgtttatatc aagtgttcct ctgaaaatac agcttgatct
    14161 atctttaaga acacctttag cctcaagcac tccgatggct gccggcgcat taaacttaac
    14221 agtatggaaa atatcgctta ccgattcatc actacccatg aacataggag cagaacgcaa
    14281 aacagagttc tttccgtcca tagagtacac ataatttgat acaaactttc cctgtcctgc
    14341 agacaattct attactttta acctggaatt ttcgctcaaa gaaaaatacc cgctttctga
    14401 tatgctatcc gcttttgcag tttttattat atttataagc acaagttctg cattacgttc
    14461 tattattgcc cgaataacac catttctgag tttatcagag tagtttccgc tgatctgcct
    14521 tattattact gctttagatc caggtttaag actgaacagg gagttttcaa atatactagt
    14581 gtcatcgtac caaagcttta ttattccagc atcctgatca tcttgtgttt ttatatagta
    14641 tccgcagtta ctgaaaagtt ctgccatgag aagatacttt ctgtcggttc cttcaaaatc
    14701 atatttcttg atgagttcaa ttccctgctc atcaatatct gaaattgtat tagagccaca
    14761 cggttttcca aagtttccag ccttgaaatc tggtctgttc agatttattc ttctgaacag
    14821 cggaaaccca atttctttgt actcatcaaa tcttttttgc tgatatcccg aaagcagagt
    14881 gtttttactt tcttttacaa tcttcttaaa cacttcttct tccgaataac tttcaaaaac
    14941 tcttttaggt tcatgaatga ctgatttaaa atttttatga cctgaccgta ctgttttagc
    15001 gtataaagta ttcatattat tcacctagcc tattgtcgat tctacttcga gattaagcag
    15061 tctgttcagt tctattgcat attctaacgg caattctttg gcaaaaggtt ctataaatcc
    15121 tctaactata attgatttag cttctttttc cgacatacct ctgctcataa gataaaaaat
    15181 ctgttcgtcg cttattctcc ctacctttgc ttcatgtcct acgtctgcat catctgtgaa
    15241 aacctctata agtggaattg tatcgcttct tgattcgtta tcaagcatta acgcattaca
    15301 tcgcacagtt gcttttgctc ctttggctct ttcgtcaact ttaagccatc ccctgtagaa
    15361 tgaccatcca cctcccaaac ttatacttct agagtctacc gttgaacttg tataaggggc
    15421 aaacataaat actttggagc ctgtgtccaa atgctggttt ggaccggcaa agcttactgc
    15481 aatcgtttcg ttttttgcgc cctttcctcg aagtatactt gtaggataag tcatactttt
    15541 atgacttccc attgaacctg taacccattc cataacgccg tcctggtcaa ctatagctct
    15601 tttcgaatta agattatacg tattcttact ccagttctgt attgtagaat actttaccct
    15661 tgcccccttc tttacaaaga tctcaaccat gcctgcatga aaatttatag cattatagta
    15721 tggagctgaa catccttcaa caaaatgtaa acggctacct tcgtcagcta ttattatact
    15781 gtgttcaaac tggcccatac cttcattgtt cattctaaaa taagcttgca aaggcaattt
    15841 tacatcgaca ttggggggta tataaagaaa agtcccaccg ctccatattg cattattaag
    15901 agctgaatat ctgtgatttg aaggagaaaa agctttcata aagtactttt tgacaagatc
    15961 cggatagttt cttacggcac tttctgtatc aagaaaaatg attccctgac tttcaagctc
    16021 ggtttttata ttatgatata ccacttcaga atcatactga gcacctactc cacttaatgc
    16081 cattctttca gcttgaggaa tcccgagctt ttcaaaagta tctttaattt cgagtggaac
    16141 gtcatcccat gatgttgcct tcttatatct tggatgaata tatgtgacta tcttgctcag
    16201 atccacatcg gaaaggtcaa ctccgaagtt aggttcatgc catgtttcat atattttaaa
    16261 ggcattgatc ctttgttcaa gcatccattg cggttccctc ttggattcag aaatctccct
    16321 tattatactt tcattaagcc ctggtataac catagatttt gcggcttgtt tatttctgaa
    16381 atcatacttt tcctgacctt tttgcagatc gtatatcata ccttgaatat cactcatgtg
    16441 catcacctgc ttttacgaag ttgtaacctt tttcttcaat ttcttttgca agagaggctc
    16501 ctgatgttgt aacaatttgc ccatctatat acacatgaac aaaatctgga ataacatagt
    16561 taagaattct ctgataatga gttataagca gcacggctgt tttttcattt tttatttttg
    16621 ttatactttg cgcaactact ttcaacgcat caatatccaa accggagtct atttcatcaa
    16681 gaatcgcaag tctaggagaa agaatgccca tctgaagtat ttctcctttc ttcttctctc
    16741 caccggaaaa accaacatta gtatatctct gaagaaattc atcacttaat gtaagatctt
    16801 tagcatagtc agagattctt gagcttaatt ttaaaaccgt tatctcgtct tccgttttca
    16861 catttctgta tgaatttatt atgaactgtc tcatctttac accgtcaatt tcttcgggca
    16921 tctgaaatga cataaaaaga cctaacttgg ctctttcatc cgtagagagt tcttttattg
    16981 atctgcccat aaaaagtatc tcaccgtttg taatctcata ttttgggttt cccattatca
    17041 cattagcaag agtagatttt cctgatccgt taggacccat cacagcatga gtttcccctt
    17101 cacttatcga aagatttatg cctttgagta tttccctacc atcttctttt atactagccc
    17161 tgagatcaaa aatttcaaga attttagaca tttttaattc ctcctatttt tatcctattt
    17221 tattaattct accatatttg tcttatttat gtcaataact tttttgataa agagacgaga
    17281 tactggttct taatttgctt agaaagtttt ttcattgata gatttggtat tttcatgcca
    17341 aaatgatata attcctactg aacagctttt aaggaggtgt ctgatgggat tttttgatga
    17401 gttgaagaaa ggactcagta aggctaaaga agcaattttt gataatataa aatcaatttt
    17461 taaaac
//
LOCUS       DAOSHR010000021        17380 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_2666_length_17380_cov_5.433766, whole genome shotgun sequence.
ACCESSION   DAOSHR010000021 DAOSHR010000000
VERSION     DAOSHR010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 17380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17380
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2666_length_17380_cov_5.433766"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="PLU28_02090"
     CDS             <1..703
                     /locus_tag="PLU28_02090"
                     /inference="COORDINATES: protein motif:HMM:NF017946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HOG33888.1"
                     /translation="LFQFTASVYIWKYILENSPQIKNYSVISLTSYLLFSTIIKAITQ
                     NTTSMDIEKDIRTGNIYNFLILPIDYIFLYLFRDIGSSLGRILLNSWLIGIYGLLFFN
                     VNIYYGIVNYLLFFISIVFSILIYSEISILIGLISFWTVSQNGLRYANSVISAFFSGS
                     LIPLDFYPDLLKKISLFLPYRSIIYVPITILQTNTIYSNLFQLLIQFLWVSVLFFINN
                     KLKIKAFNSLEIVGG"
     gene            705..1511
                     /locus_tag="PLU28_02095"
     CDS             705..1511
                     /locus_tag="PLU28_02095"
                     /inference="COORDINATES: protein motif:HMM:NF017946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HOG33889.1"
                     /translation="MIKKALKYISLYFKFNLLYIKTQLMYPKDFIIWFIFGSFSTIIN
                     IFFMKVIYYNTPLLGNWDYYQTLMILGYSSTSIGLFNIFFINIFSLGSSYIIEGKLDK
                     FLIRPINVLFQLLVERIDLDNISELIIGVFLIVYSALKIGYEINVFLILFSIVFIIFS
                     VFTLLGLFLIFASTNFWLQEKINFIPILYSLFNFSRFPITIFNKKIKLILTFIMPYFY
                     LGYYPVNFLLKIDTKYVYKIFYQPILCLIFLTIGGIVWKIGLSKYESTSI"
     gene            complement(1676..4021)
                     /locus_tag="PLU28_02100"
     CDS             complement(1676..4021)
                     /locus_tag="PLU28_02100"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3656 domain-containing protein"
                     /protein_id="HOG33890.1"
                     /translation="MSIELLAPAGNMNCLYAAVQNGADAVYIGGKDFNARQYADNFDR
                     EQLVQAVQYCHVRGVRVYVTINTLLSDTELKEILDYLIFLYNADVDALIVQDLGLVWL
                     IRSLIPDFELHASTQMTVHNLYGARFLEKLGFKRVILSREMSLQEIEHITRNSGIETE
                     IFGHGALCFSYSGQCLMSSFIGSRSGNRGKCAQPCRLEYSLLENGKEVSFPFKHLLST
                     KDLNTSEIMPEILKSGIKSLKLEGRMKKAEYVATVVRVYKKILNSAPPDDSDKKDLIK
                     IFNRQFTTAYLKNPLNSVVAPERPDNRGIYIGKILCVSEKIQIKLEDTLSVGDGIRNA
                     GKENNGWTVREILSNNCKINSAEKGDTVWINFDGNPKTEDHLYKTSDSLLLKNASSSF
                     QQDLRKIELKGFFKAKINDKIFFIVEDDFGNNIFCDSQETVQKAVKTPTSKERILQQL
                     NKCGNTPYVFKNIYLDCDNEISVPISSINEIRRTALEKITELRKNFNRREELKTLNSD
                     TLNMDFIYDDSLPLLTVSAYNEEQMKAAIDEGITRIYSYDLNIYKKYLSQALKKGIQL
                     IPALGRITQDEEIQKIKETIDSKELLIGNIGHIAAFDDKKISCDFSMNVFNTAGISFL
                     RENGASMITLSPEMDIKQINKTAKHFNGLEVIVYGRIPLMISKHCVINRVKNMSTRKC
                     GLCKSADYSLKDRINTVFPIITDSACRMTVLNSVALCAIQFISELKPKYLRIFLTKEN
                     YSQSTEIIKAFKNAVSGKDFSDFSEKMQKEGFTKGHFFREV"
     gene            4365..5624
                     /locus_tag="PLU28_02105"
     CDS             4365..5624
                     /locus_tag="PLU28_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     II"
                     /protein_id="HOG33891.1"
                     /translation="MNRVAVTGVGSINPLGKTKSEFIENLKKMKTGTGYITQFDSTGH
                     KVKVAAEIKDFDPCEYMDKKSARRYDRVLQLAVAASKKALEDSGLEPEGVWRDNAAVI
                     VASGIGGFKTLYREFEVMEKKGPKAVSPYLIPMMIADMPSGVVSMELGLKGPNFSTVS
                     ACASSLHAIIISSMMIMHGYIDAAFAGGAEATIDPLPVAAFANMTALSQSEDPLTACR
                     PFDRQRDGFVMGESSGVLVLENYEHALKRGAKIYGFINGFGMTGDAYHLSATDPQGIQ
                     AARAMKLAMEMSGIGPEDIDLVSCHATSTPVGDLSEIRAVKKALGESSKKVVIQASKA
                     LLGHGLGAAAATELIGAILQAQEGFVHGMPNLMEPDDEFAGLNMPRQTVNAPIKVILK
                     NSFGFGGHNAALVYTKNQDYGIKGEEN"
     gene            5621..6718
                     /locus_tag="PLU28_02110"
     CDS             5621..6718
                     /locus_tag="PLU28_02110"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HOG33892.1"
                     /translation="MKGLNINGLESKIPLIQGGMAVGISLDRLSSAVANEGGIGVIGT
                     AGIGMFFEEYGKDYKAACIEGLKKVITGARQKTSGIIGVNIMAALSNYSDMVRTAVEE
                     KIDLIISGAGLALDLPSYLMAGTKTRIIPIVSGLRAATLIFKRWFSRYGYIPDAFIVE
                     GPKAGGHLGYKKENIYSEEHQLENTVPQVRELCEKIKREFGKEVPLIAAGGVFDHNDV
                     QAMLKLGADGVQAGTVFIATHECDADDRFKNAVVNADKEDIIIIDSPVGMPGRAVRNK
                     FLEDVGKGIKKPVNCAYRCIKTCNMKDAPYCIARALSNAARGDMENGFAFIGQEGFRV
                     NQISTVKEVIERLFPGYEKESYLGGKNGTEN"
     gene            6702..7145
                     /gene="fabZ"
                     /locus_tag="PLU28_02115"
     CDS             6702..7145
                     /gene="fabZ"
                     /locus_tag="PLU28_02115"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HOG33893.1"
                     /translation="MELKIEQIMKILPHRYPFLLVDRVIDYSDEKITAIKNVSVNELF
                     FCGHYPQYPIMPGVLLIEGLAQTAGIMILKSMDAKNISPLFLGIDSARFKKEVRPGDT
                     IMYEVYITQRRSSVIKAKGNVTCEGVICAAAELMLGMKYDGRKEE"
     gene            7142..8080
                     /locus_tag="PLU28_02120"
     CDS             7142..8080
                     /locus_tag="PLU28_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HOG33894.1"
                     /translation="MNFRNTRITELLKIKYPIIEGGMAWVGTSELAAAVSNAGGLGTI
                     GSGSMDRQLLTQEIRKIKNLTDKPFAVNIMLLNPHAPELVETVIEEKVPCVVFGAGNP
                     ATFLPALKKNGTVSMAVVSSENLAVRLERSGIDAIIGEGMESGGHIGEVTTMVLIPKL
                     MDCLSVPIVAAGGIADGRAMAACFALGAQGVQMGTRFIASKECLVHEEFKNKIIKAGI
                     RDTVITGQKLGHSARVIKTNFAKKVCQLELTCPEQAEEVLIGSLKRAVLDGDVQAGAF
                     MAGQCSGLIENIEGVGDIMEKMIFQAEKIFCSGQEV"
     gene            8084..8962
                     /gene="fabD"
                     /locus_tag="PLU28_02125"
     CDS             8084..8962
                     /gene="fabD"
                     /locus_tag="PLU28_02125"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HOG33895.1"
                     /translation="MTSIVFPGQGSQYLGMGKEVLETNPEYYEYLSKVKELSGIDIQK
                     IIFASSEEELTQTQNAQLAILCISYIKFLYATRKQGIKPDVVAGHSVGEWTALVACGA
                     VSFEQAVSMVYLRGKYMSQSESNNQGSMAAVLGLDSKKVTAELKKYPDVVVANYNSPV
                     QIVISGEKTQLLNALKDLSSAGAKKVVQLKVSGPFHSPFMEQAAQRLAQDIKNIEFYD
                     PEVPIVQNVSAKFETDKRIIKENIIQQITSPVRWTESIQTKLSAGVKKFVEAGPGKVL
                     TGLIRAISKEPELINI"
     gene            8974..9711
                     /gene="fabG"
                     /locus_tag="PLU28_02130"
     CDS             8974..9711
                     /gene="fabG"
                     /locus_tag="PLU28_02130"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HOG33896.1"
                     /translation="MKLQGMTAVVTGAGRGIGEKITELFIDNGATVAGIDLDEDGLQA
                     QQQKYGEKFIPFICDITKSEDVDKTVRSIAEKTLKIDILVNNAGVTRDNLFVIMKEEE
                     FDFVINVNLKGTFLMSKAVAKIMRKQKTGSIINISSVVGVEGNVGQANYAASKAGVIG
                     LTRTLARELTLKGEEIRVNAVAPGFIMTSMTESLKDEYRSAIIGRSCIKRLGNPLDVA
                     KTVLFLASQDSSFITGQVIRVDGGISI"
     gene            9738..9977
                     /gene="acpP"
                     /locus_tag="PLU28_02135"
     CDS             9738..9977
                     /gene="acpP"
                     /locus_tag="PLU28_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOG33897.1"
                     /translation="MNKAELFDKVRDIISEKLDVEKDKVTLQAVLTDDLGADSLELVD
                     LAMEIESSLGTAIADSELEKIKTVGDVVETLLKKM"
     gene            10140..11021
                     /locus_tag="PLU28_02140"
     CDS             10140..11021
                     /locus_tag="PLU28_02140"
                     /inference="COORDINATES: protein motif:HMM:NF015132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOG33898.1"
                     /translation="MGKTTLKVALCGTVTSVVFGLSFLFTKNALDFVNVFDFLAIRFT
                     VSFVSMMAIMFFLKVRVTRKPYYKLLPLAIFQPVLYFIFETLGLVHTSSSQAGIIVST
                     LPIFTLIASRIFLRERIKPAQVIFMVLSLLGALMIIGINLSDSGILGDMYLLFAVFSA
                     VGYSLLSKKLSAQFNPFEITFFMMFCAMIFFNAAGLIDNGIDYGIMLNPQVLAAALYL
                     GVLSSAVNFILLNYMLSKVSPIVSSIFGNLTTVVSVAAGALIRGEALFWYHICGMVFI
                     IAGVMGINWISSRKAKD"
     gene            complement(11011..12351)
                     /locus_tag="PLU28_02145"
     CDS             complement(11011..12351)
                     /locus_tag="PLU28_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG33899.1"
                     /translation="MSSLKKLLLIFFILSSSFIFFSCMLFKKPQTVDGAYILVINQST
                     DTAVKQDTGVVYSSETEGMYPSAQIFRNSEDKSIYPIDRIIDTDMLLTNRIPILTSKS
                     EPPKLNDLKDFITENYETGKKDTVRARLLSMTDHCYVWVEVSYPAKITSGQTDKLAAE
                     FESRIYPLIYDHFVEKTDLESIGAVYLLCYDIKDGFSGSGGFVAGYFSPEDLSNDSTS
                     NKCPLLYIDTYPLMTDRFGVYKIENAFSTVTHELQHLSNFYVNSIYVNYIKEEPAMQS
                     LWMDEGLSMAAEDLYNGTQTSRITYYNGSDNIRNGKVPLIQNRPDLFWLDNYSLSYLF
                     FQYLYSQTADSTLYKKIIYSPYNDFKALEEACGKSISELMTDFRIALFLNKPSGKYGF
                     NSKEGFDGIAALTSYNYERYLEASAGFVIKAPADRSFELPEKYGPDIRFYGINP"
     gene            12468..12914
                     /locus_tag="PLU28_02150"
     CDS             12468..12914
                     /locus_tag="PLU28_02150"
                     /inference="COORDINATES: protein motif:HMM:NF001095.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HOG33900.1"
                     /translation="MKILVDGDACPVRELTESNAKKHGIAVMYFINDSHIVHSEYSKV
                     ITVSSGRDVADFEIIKHACAKDIIITNDYGLAAMALAKRTYVLSFSGLLYDESNIDSL
                     LMQRHIGMKERRNGNYTKGPKKRTKQDDLNFDAALNRLIEKQISQE"
     gene            complement(12915..15053)
                     /locus_tag="PLU28_02155"
     CDS             complement(12915..15053)
                     /locus_tag="PLU28_02155"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOG33901.1"
                     /translation="MKKLKKEVTLQGLDCANCARKIEERVNKLEGVDKATLNFMTKTL
                     SIEVSGQENIAAIMLKTNSIIRKLEPDVKVVETTSNSAEKSGDEPGKLSKKEVYTFAA
                     GVAVFLFAILMGNSPYISVFLYAGAYIITGWDIIIKAYKGVSAKNIFNENFLMTVASL
                     GAFAVKEYPEAVAVMIFYKIGEFFQDMAVNNTKNSIKALLDLKPEYVNILKDGKTVQV
                     SPQTIRTGDEFVVKPGEKIPLDGVIVHGSSYADTSAITGEYMPVGIVRGDEVLSGYVN
                     QSSLITVKASRPFTESTVSKIIEMVQNSAEKKSKTEQFITKFARYYTPAVLLAALLTA
                     VIPPVFLGQPFSEWLYRALIFLVVSCPCALVLSVPLAYFAGAGVASKKGILLKGTNYL
                     EGLNAIDSVLFDKTGTLTSGELEITSIIPAEGYTPQSLLFYAAVCEVHSSHPVAVSVM
                     KKYTKDYGTIDENKIEKYEEVMGKGIKATYEGIELVCGRYEYLKESIAVSDAEFTGKN
                     DDRTCIYVGISGNYAGKISFSDSVKPQTVQTIEFFADQKIETYILTGDKTESALEVAS
                     QLGIKKENVFSRLLPGQKLEVLDKILLRKKKNHSVVFVGDGINDSPVMAKADISISMG
                     KAASQAAVQASDIVIMNDNPASIITAVKIARITQKTAITNIFFSIAVKVLVIVLSTFG
                     LANMWHAIFADVGVALIAVLNSSRILFKKI"
     gene            complement(15061..15423)
                     /locus_tag="PLU28_02160"
     CDS             complement(15061..15423)
                     /locus_tag="PLU28_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005903515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOG33902.1"
                     /translation="MEYEEKIPSCDCVSEHKDIVEKVRKEMSDDEIIYDMAELFKVFG
                     DSTRVKILDALFVSEMCVCDLASVLEMKQSAVSHQLRILKQARLIKFRKEGKAVYYSM
                     ADEHVRKIFDQAINHLEE"
     gene            complement(15573..16061)
                     /locus_tag="PLU28_02165"
     CDS             complement(15573..16061)
                     /locus_tag="PLU28_02165"
                     /inference="COORDINATES: protein motif:HMM:NF021028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HOG33903.1"
                     /translation="MPNDKDLRTVTFNSNGGSTQAVPQTLKVHSGQPIINLPQPPVRG
                     RLSFESWDTKADGSGYTVDAMTKIEEDLTVYAQWWLSSYSYGQNKNKGIKKISWFPYN
                     NYEYIVIHVETDPGYAIDKIDIKMDGHDYTTTQEWSKILFDPFLDSMFKLTVSAVATP
                     TT"
     gene            complement(16224..16508)
                     /locus_tag="PLU28_02170"
     CDS             complement(16224..16508)
                     /locus_tag="PLU28_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG33904.1"
                     /translation="MIDPLEYYDDFYLQENAFYALHRTNEDDAFSDSIAMESLNYAQL
                     KRDEKTNEYYFDLFHERIDLETAINLSREGTLIFDFIGTDKEYDGFLSRI"
     gene            complement(16603..>17380)
                     /locus_tag="PLU28_02175"
     CDS             complement(16603..>17380)
                     /locus_tag="PLU28_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG33905.1"
                     /translation="LRIKKLPKNTVKQKADKIIELTGLKGMEKRRPDQISGGQQQRVA
                     LARCIVMEPKVLLFDEPLSNLDAKLRENMRLEIRRIQKELNITSIYVTHDQTEAMSIS
                     DKVVIMDKGKIMQVGSPFEVYAYPENKFVADFIGTVNIIKAKVIAQEGSQIKVFSKEL
                     NQTFYGITKKAFKEGDDANIILRPQAINLEKEGRENMLCGKIDKYVFLGSHFEYDVRL
                     CGGQNIHVLSYNSMENQLPVLNQPINLYFGQKSAWIIDNV"
BASE COUNT         5317 a         3059 c         3517 g         5487 t
ORIGIN      
        1 attgtttcaa tttacagctt cggtatatat ttggaaatat attttggaaa atagcccgca
       61 aataaaaaat tattctgtta tttcactaac tagttatttg ctattttcta caataatcaa
      121 agctataact caaaatacta caagtatgga tattgaaaaa gatataagaa ctgggaatat
      181 atataacttt ttaattttac caatagatta tatttttctt tatttattta gagatattgg
      241 ctcatctctt ggaagaatac ttttaaattc atggttaata ggtatttatg ggttgttgtt
      301 ttttaacgtt aatatatatt atggcatagt aaattattta ttatttttta taagtatcgt
      361 tttttccata ttaatatatt cggaaatttc catattaata ggtttgatta gtttttggac
      421 agtatctcag aacgggctaa gatacgcaaa tagcgttatt agtgcatttt tttctggcag
      481 tttaataccc ctagattttt atcctgactt attaaaaaaa atatctttgt ttttaccgta
      541 tagatcaatt atatacgtac caataacaat attacagaca aatactatat attcaaactt
      601 gttccaattg ctaatacagt ttttatgggt ttctgttctg ttttttataa acaacaaatt
      661 aaaaataaaa gcttttaact ctttggagat tgtaggggga taatatgata aaaaaagcat
      721 taaaatatat aagtttatat tttaagttca atcttttgta tataaaaact caattgatgt
      781 atccaaaaga ttttataatc tggtttattt ttggttcttt ctcaactata ataaatattt
      841 tttttatgaa ggttatatac tataatacac ctctattagg aaattgggat tattatcaga
      901 ccctcatgat tttaggatat tcaagcactt caataggatt attcaatata ttttttataa
      961 atatttttag tttagggagt tcttatataa tagaaggaaa attagataaa tttttaatca
     1021 gacctattaa tgtattattc caattattag ttgaaaggat tgatctagat aatatatctg
     1081 aattaataat aggtgttttc ttaatagtat atagtgcatt aaaaatagga tacgagataa
     1141 acgtattttt aatactattt agtattgttt ttataatttt ttctgttttt acattactag
     1201 gtctattttt aatttttgct tcgacaaact tctggcttca ggaaaaaatt aattttatac
     1261 ctatactata ttctttattt aatttttcaa gatttccaat tactattttt aataaaaaaa
     1321 tcaaattaat tttgactttt ataatgcctt atttttatct agggtattat ccggttaatt
     1381 ttttgcttaa gatagataca aaatatgttt ataaaatttt ctatcagcca attctttgct
     1441 taatattttt aactattgga ggcatagtat ggaaaatagg actatcaaaa tacgaaagca
     1501 caagcattta ataaaattag taaagccagt tataataaca gacagtactg atttaagaat
     1561 aatttggaaa tgttttggaa ttattttccc aaaataaaaa gtaatataaa aattctatct
     1621 atggattata aagtcacact ttcgcaggtt agttcagata acatgattat tattattata
     1681 cttcccgaaa aaaatgacct ttggtaaatc cttccttctg cattttttct gaaaaatccg
     1741 aaaaatcctt tccggagact gcatttttga aggcctttat aatttcagtg ctctgcgaat
     1801 aattttcttt ggtcagaaag atccttaaat atttaggttt caactcactg ataaactgta
     1861 ttgcacaaag tgctactgag ttaagcacag tcattctgca ggcggaatcg gtgattatag
     1921 gaaatacagt atttattctg tcttttaaag aataatcagc gcttttacac agtccacatt
     1981 tacgtgtgga catatttttt actctgttta taacacaatg tttggatatc atgagcggaa
     2041 tcctgccgta aacgattact tcaagaccgt tgaagtgctt tgccgtttta tttatctgct
     2101 ttatatccat ttctggggag agagtaatca tacttgcgcc gttttccctg agaaaggaga
     2161 ttcctgcggt gttaaaaaca ttcattgaaa agtcacagct tattttttta tcatcaaaag
     2221 ctgcaatatg tcctatatta ccaattagca gttcctttga atcaatagtt tcttttatct
     2281 tttggatttc ttcatcctgt gtgattcttc ccagtgcggg tatcaactgt atcccttttt
     2341 tcaaagcctg ggaaagatat tttttataaa tattaaggtc atatgaataa attctagtta
     2401 tgccttcatc aattgctgcc ttcatctgtt cttcattgta tgctgaaaca gtaagcaaag
     2461 gcagactgtc atcgtatata aaatccatat tcagagtatc ggagtttaag gtcttcagtt
     2521 cctctctgcg gttgaagttt ttccgaagct cggttatctt ttcaagtgca gttcttctga
     2581 tttcatttat ggaagatata ggcaccgata tctcgttatc acagtccagg taaatatttt
     2641 tgaaaacata aggagtatta ccacatttat taagttgctg aaggatacgt tcttttgaag
     2701 ttggtgtttt gacagccttt tgaacagttt cctgcgagtc acagaaaatg ttgtttccaa
     2761 aatcatcttc aacaataaaa aatattttat catttatctt ggctttgaaa aatcctttaa
     2821 gctctatttt cctcaaatcc tgctgaaatg aggaggaagc attttttaac agcagactgt
     2881 ccgatgtctt gtaaagatgg tcttctgttt ttgggtttcc gtcaaagttt atccagaccg
     2941 tgtctccttt ttcagctgag ttgattttac agttgtttga aagtatttct ctaacagtcc
     3001 agccattatt ttcttttccg gcatttctta tgccatcgcc cacagataat gtatcctcaa
     3061 gcttaatttg gattttttca gagacacaaa gaatctttcc tatataaatc cctctattgt
     3121 caggtctttc gggagcaaca acagagttaa gaggattttt aagatatgcg gttgtaaact
     3181 gacggttaaa aatttttatc aggtctttct tgtcggaatc atctggagga gcagagttta
     3241 gaattttttt gtatactctt accacggttg ccacatactc cgcttttttc attcttcctt
     3301 caagcttaag ggactttatt ccgcttttaa gtatttccgg cataatctct gaggtgttga
     3361 ggtctttagt acttagaaga tgtttaaaag gaaaagagac ctctttgccg ttttcaagga
     3421 gagaatattc tagcctgcat ggctgagcgc attttcctct gttgccgctt ctgctgccta
     3481 tgaaactgct catgaggcac tgacccgaat aactaaagca tagtgctccg tgtccaaaaa
     3541 tttcagtttc tatacctgag tttctagtta tatgttcaat ttcctgaagt gacatttctc
     3601 tggaaagtat tacccgctta aagccgagtt tttcaaggaa acgggctcca tacaggttat
     3661 gtacggtcat ctgggtgctt gcgtggagct caaagtcggg aattaaacta cgtataagcc
     3721 atacaagccc aagatcctgc actataaggg catcaacatc tgcgttgtaa agaaaaatca
     3781 gataatcaag gatttctttc agttcagtat cgcttaaaag agtatttata gtgacatata
     3841 cccttacgcc tcttacatgg cagtactgaa cagcttgcac cagctgttct ctgtcaaaat
     3901 tatcggcata ctgtctcgca ttaaaatcct taccgccgat atagacagca tcggcgccgt
     3961 tctgaaccgc tgcatacaga cagttcatgt tgccggcagg agcaagaagc tctatactca
     4021 tacaatcact ccatatttat catattcgga tatatttaac catttaattt tttcagataa
     4081 ataaccaaat taataactgt ttacacaata atattcataa gaataaaaaa gaatagtata
     4141 aagctacaga gcattctatt ttggacaggc ttgacaagaa aaaaacgata tgttataatg
     4201 actgatagtc attatttctt tgtaaagaat gctttgtcat ttatgtggat gtgtctgtta
     4261 aatgctcata atcactactg gagaagatat ttccggtttt tattttaaaa tattatgact
     4321 atcagtcata atataaaatt aacaaatttg aaggaggaaa agaagtgaac agagttgccg
     4381 ttacaggagt tggaagtatt aatcctctcg gaaagacaaa aagtgagttt attgaaaacc
     4441 taaaaaaaat gaaaacagga acaggatata taacacagtt tgacagtaca ggtcataaag
     4501 taaaggtggc tgcagaaata aaagattttg acccatgtga atatatggat aaaaaaagtg
     4561 ccagaagata tgacagagtt ctgcagctcg ctgttgcagc atccaaaaaa gcgttggaag
     4621 attccggtct tgaaccggag ggagtatggc gcgataacgc tgctgtaatt gttgcatcag
     4681 gtataggagg attcaaaact ctttacaggg agtttgaagt catggaaaag aagggaccca
     4741 aagcggtaag tccgtacctt attcctatga tgatagcaga tatgccttca ggagtagtat
     4801 ccatggagct ggggctcaaa ggacctaatt tttctactgt aagtgcatgt gcatcatcgc
     4861 ttcatgccat aataatctcc tcaatgatga taatgcacgg ttatatagat gctgcttttg
     4921 ccggaggtgc agaggcgacc atagatcctt taccggtagc cgcatttgca aatatgacgg
     4981 cactaagcca gtctgaagac cctcttactg catgtcgtcc ttttgacaga caaagagacg
     5041 ggtttgttat gggtgagtcg tcaggcgtgc ttgtgcttga aaattatgag catgcgttga
     5101 aaagaggggc aaaaatatat ggattcataa acggtttcgg tatgacaggg gatgcttatc
     5161 atctaagcgc tacagatccg cagggaatac aggcggcacg ggcgatgaaa ctggcgatgg
     5221 agatgtcggg tattggtcct gaagatattg atctggtaag ctgtcatgct acttcaactc
     5281 cggttggtga tctttcggag ataagagcgg taaaaaaagc tttgggagaa agttctaaaa
     5341 aagttgttat acaagcttcc aaggctcttt tagggcatgg gctgggagcg gcagcagcaa
     5401 cagagctcat aggggcaatt cttcaggcac aggaaggttt tgtccatgga atgcctaatc
     5461 ttatggaacc tgatgatgag ttcgcagggc ttaatatgcc cagacaaaca gtaaatgcac
     5521 caataaaggt aatactgaaa aattcatttg gctttggcgg tcataatgcc gcactggtat
     5581 atacaaaaaa tcaggattat ggcattaaag gagaagaaaa ttgaaaggtt taaatataaa
     5641 cggtcttgaa tcaaagatcc ctttgattca aggtggtatg gccgtaggaa tatcactgga
     5701 tagactatcc agtgcagttg ctaatgaagg gggaataggt gtaataggaa cggccggcat
     5761 aggaatgttt tttgaagagt atggaaagga ttataaagcg gcctgtatag aaggactgaa
     5821 aaaagttata acaggtgcaa ggcaaaaaac ttcaggcatt atcggcgtaa atataatggc
     5881 agctctttca aactattcgg atatggtaag aacggcagtt gaagagaaga tagatctaat
     5941 aatatccggt gcggggcttg cacttgacct tccttcctat cttatggcag gaacaaaaac
     6001 cagaattatt cctatagttt cagggctgag ggcagccact ctcatattta aaagatggtt
     6061 ttcacgttac ggttatattc ctgatgcctt tatagttgaa gggccaaagg cgggagggca
     6121 tctcggatat aagaaagaaa acatttattc ggaagaacat cagcttgaaa acactgttcc
     6181 tcaggtacgg gagctatgcg aaaaaattaa aagagagttt ggcaaagagg ttcctttgat
     6241 agcagccgga ggtgtttttg accataatga tgtacaagct atgcttaagc ttggcgcaga
     6301 tggagtccag gctggaacag tttttattgc gacgcatgag tgcgatgccg atgacagatt
     6361 taagaatgcg gtagtcaatg cagataaaga ggatattatc attatagaca gtccggttgg
     6421 aatgccagga cgtgcagtca gaaataagtt tcttgaggat gtcggcaaag gcataaaaaa
     6481 gcccgtaaac tgtgcttaca gatgtataaa aacatgtaat atgaaagatg cgccatactg
     6541 tatagccaga gcactttcaa acgccgcaag gggtgatatg gaaaatggct ttgcctttat
     6601 agggcaagag gggtttagag tgaatcaaat atccacggtg aaagaagtga tagaaagact
     6661 ttttccgggt tatgaaaaag aaagttatct aggaggaaaa aatggaactg aaaattgaac
     6721 agattatgaa aatacttccc cacagatatc cgtttcttct tgtggatagg gtaattgatt
     6781 attctgatga aaaaataacc gctatcaaaa atgtttctgt gaatgagctt tttttctgcg
     6841 gtcactatcc gcagtatcct ataatgcccg gagtacttct tatagaaggg ctcgctcaga
     6901 ctgcgggaat aatgatacta aaaagtatgg atgcaaaaaa tatttctccg ctttttttgg
     6961 gtatagacag tgccagattt aagaaagagg tacgacccgg tgatactata atgtacgaag
     7021 tgtatattac tcagaggaga agctctgtaa taaaggcaaa aggtaacgta acctgtgaag
     7081 gagttatctg tgcggctgca gagctgatgc ttgggatgaa atatgatggg aggaaagagg
     7141 aatgaatttc agaaacacaa gaataacaga acttttgaaa ataaagtatc ctataatcga
     7201 aggcggaatg gcatgggttg gaacctcgga gcttgcggct gctgtttcca atgccggagg
     7261 gttaggaaca ataggttcag gctcaatgga caggcagctg ctgacacaag agataagaaa
     7321 gattaaaaac cttaccgata agccttttgc agttaatata atgcttctta atccgcatgc
     7381 tccggagctt gttgagactg ttattgaaga aaaagttccg tgtgttgtct ttggtgcagg
     7441 aaatcccgcc acatttcttc ctgcattgaa aaaaaacggt acagtaagta tggcagtggt
     7501 atcttccgaa aatcttgcgg tcagacttga aagatctgga atagatgcaa taataggtga
     7561 aggaatggag tcaggcgggc atataggtga ggttacaaca atggttctca ttcccaagct
     7621 gatggactgt ttatcggtac cgattgttgc ggcagggggt attgccgatg gcagagcaat
     7681 ggctgcgtgt tttgcgcttg gtgctcaggg agtgcagatg ggtacaagat ttatagcttc
     7741 aaaagaatgc cttgtacatg aagagtttaa aaataaaata ataaaagcag gaataagaga
     7801 cacggtgatt accggtcaaa agttaggaca tagtgcgagg gtcataaaaa caaattttgc
     7861 aaaaaaagta tgccagcttg agcttacttg tccggaacaa gcggaagaag tattgatagg
     7921 aagtctgaaa agggcggtac tagatggaga tgttcaggcg ggtgcgttta tggcaggcca
     7981 gtgcagcggt ctgatcgaga atattgaagg tgtaggggat attatggaaa aaatgatatt
     8041 tcaggcagaa aaaatattct gttccgggca ggaggtataa aaaatgactt ctatagtctt
     8101 tccagggcag ggatctcaat acttgggcat gggtaaagaa gtgcttgaga caaatccgga
     8161 gtactatgag tatcttagta aggtaaaaga acttagcgga attgatatac agaagataat
     8221 tttcgcaagc agcgaagaag agcttaccca aactcagaat gcccagttgg ctatactttg
     8281 cataagttat ataaaatttt tgtatgcaac ccgtaaacaa gggattaaac ccgatgtggt
     8341 tgccgggcat tcggttggag aatggacagc gcttgttgcc tgcggggctg tcagctttga
     8401 acaggcggtg agtatggtat atctcagagg aaagtacatg agtcaatcag agagtaacaa
     8461 tcaggggagt atggcagcgg ttcttggtct tgattcgaaa aaagtaactg cagagcttaa
     8521 aaaatatccc gatgtagtgg ttgcaaacta taactccccg gtgcagatag ttatcagcgg
     8581 tgaaaaaacc cagcttctta atgcattgaa agatctcagc tcagcaggag ctaagaaggt
     8641 tgtgcagctt aaagtaagcg gaccgtttca ctctcctttc atggagcagg cagcgcaacg
     8701 tcttgcacag gatataaaaa atatcgaatt ttatgatcct gaagttccaa ttgtgcaaaa
     8761 tgtaagtgct aagtttgaaa cagataaaag gatcataaag gaaaacatca tacagcagat
     8821 aacttctccc gtaaggtgga ctgagtcaat tcagacaaaa ctttcggcag gagtgaaaaa
     8881 atttgttgaa gccggccctg gaaaagtcct tacgggactt ataagggcta taagcaaaga
     8941 acctgagtta ataaatatat aaggaggtgc gggttgaaac ttcaaggaat gacagccgtc
     9001 gttacaggcg caggaagagg tataggtgaa aagataaccg aactttttat agataatgga
     9061 gcaacagttg caggaataga tcttgatgaa gacggattgc aggcacagca gcagaagtac
     9121 ggtgaaaagt ttattccttt tatatgcgat ataactaagt ccgaagatgt ggataaaaca
     9181 gtaaggagta ttgctgaaaa aacattaaaa atagatatcc ttgtaaacaa tgcaggagta
     9241 acaagggaca atctttttgt cataatgaag gaagaagagt ttgattttgt tataaatgtg
     9301 aatcttaaag gaacttttct tatgagcaaa gcggttgcaa aaataatgag gaaacagaaa
     9361 actggaagta tcataaatat ctcaagcgtg gttggagtag agggaaatgt tggacaggca
     9421 aattatgcag catccaaagc tggggtaata ggactgacca gaacgcttgc cagagagcta
     9481 acccttaaag gcgaagagat aagggtaaat gctgttgccc ctggttttat aatgacaagt
     9541 atgacagaga gccttaagga tgagtataga tcagctataa tagggcgctc atgtataaag
     9601 agactcggca atccccttga tgttgctaaa acagttcttt ttctagcctc acaggactct
     9661 tcatttataa caggacaggt aataagggta gacggaggaa tctccatata aaatattaaa
     9721 atgcacagga ggaaaaaatg aacaaagcag aactttttga taaggtgaga gatattattt
     9781 ctgaaaaact ggatgttgaa aaggacaaag ttaccttgca ggcagtgctt acagatgatc
     9841 tcggggcgga ctcgctggag ttggtagacc ttgccatgga gatagaaagc agtctgggaa
     9901 cagccatagc cgacagtgag cttgaaaaaa taaaaactgt tggagatgtg gtggaaaccc
     9961 ttctgaaaaa gatgtaaaaa ataaaaccat gcttctgtta aaaggagcat ggtttttata
    10021 aagtatgata caaagtatta ttaacataaa aagcagtttc ttttaaaacg gtatatgata
    10081 aaatgctttt attaattagc ctgcttaacc attttttgtt aagcaaaaac gggggattta
    10141 tgggtaaaac aactttgaaa gtcgcattat gcggcactgt aacctcggtg gtattcggtc
    10201 tttcctttct ttttacaaag aatgcactgg attttgtcaa cgtttttgac tttttggcaa
    10261 taaggtttac ggtttctttt gttagtatga tggccataat gttttttcta aaagtcagag
    10321 taacaagaaa accttactat aaacttttac ctcttgcaat atttcagcct gttctttact
    10381 ttatatttga aacattggga ctggttcata catcaagttc ccaggccgga ataatagttt
    10441 ccacacttcc aatctttacc cttatagcct caaggatctt cctgcgtgaa aggatcaaac
    10501 ccgctcaagt tatattcatg gttcttagct tattaggcgc acttatgata ataggaataa
    10561 atctatcaga ctcaggtatt ctgggcgata tgtacctttt atttgccgta ttttcagcgg
    10621 tcggatactc acttctttca aaaaagcttt ctgcacagtt caatccgttt gagattactt
    10681 tttttatgat gttttgtgcc atgatctttt tcaatgcagc agggcttatt gataatggta
    10741 tagattacgg tattatgctt aacccacagg tactggctgc cgcactttat cttggagtcc
    10801 tttcatcagc agtcaacttc atactgctta actatatgct gagtaaagta tcccccatag
    10861 tatcctctat ctttgggaat cttacaaccg tggtttcagt ggcagcagga gcactgataa
    10921 gaggagaagc acttttttgg tatcatatat gcggaatggt ttttataata gccggggtaa
    10981 tgggaataaa ctggatatct tcacgtaagg ctaaggattg attccgtaaa atctgatatc
    11041 cgggccatac ttttccggaa gctcaaaaga cctgtcagca ggagccttaa taacaaaacc
    11101 ggcgcttgcc tcaagataac gttcatagtt atatgaggtc aaagcagcta tgccatcaaa
    11161 accctcctta ctgttaaaac catatttccc ggatggttta ttcagaaaca gggcgattct
    11221 gaaatcggtc ataagctcag agatactttt tccgcaagct tcctcaagag ctttaaaatc
    11281 attgtaagga ctgtatataa tttttttgta gagtgtactg tcagctgttt gtgaataaag
    11341 atactggaaa aacaggtaag ataggctgta attatccagc cagaacaagt cgggtctgtt
    11401 ctgtataaga ggaacctttc cgttgcgtat gttgtccgaa ccgttatagt aagttattct
    11461 tgaagtctgt gttccgttat aaagatcctc ggctgccatg gaaagccctt catccatcca
    11521 caaactttgc atcgcaggct cttcttttat atagtttaca tatatagagt ttacataaaa
    11581 gtttgacagg tgctgaagtt cgtgcgttac ggtagaaaaa gcattttcaa ttttatatac
    11641 tccaaatctg tccgtcataa gaggataggt gtctatatat aataatggac atttgttaga
    11701 ggttgaatcg tttgaaagat cctccggaga aaagtatccc gccacaaaac ctccggaacc
    11761 gctgaagccg tcctttatgt cataacagag cagataaact gctcctatag attcaagatc
    11821 tgttttttcc acaaagtgat catatattaa aggatagata cggctttcaa actccgcggc
    11881 cagtttatcg gtctgaccag atgttatttt tgccggataa gatacttcta cccagacata
    11941 gcagtgatct gtcattgata gaaggcgagc ccgaactgta tcttttttac ccgtttcata
    12001 gttctctgta ataaaatctt ttaggtcatt tagttttgga ggttcactct tggaagttag
    12061 gataggtatt ctattggtca gcagcatatc tgtatcgata attctgtcta tagggtatat
    12121 acttttatct tcactgttcc tgaaaatctg tgcagatgga tacattcctt ctgtttcact
    12181 gctgtacact acgcccgtat cctgtttcac ggccgtatcg gtgctctgat ttataacaag
    12241 gatatatgcc ccgtccacgg tctggggttt tttgaaaagc atacatgaaa aaaagatgaa
    12301 agaagatgaa agtataaaaa agataagcaa gagttttttt aggctgctca taagtgaccc
    12361 cttttctatg gtacttaaat tataactctt tcaaatccat tttatagcag tatgtattta
    12421 aaagtaaaac ccggctctta tggccgggtg ataaaggaga tgtatttttg aaaattcttg
    12481 ttgatggcga tgcctgcccg gtaagggagt taacggaatc aaatgcgaaa aagcatggga
    12541 ttgctgttat gtactttata aacgatagtc atatagttca ttctgagtat tctaaagtaa
    12601 taactgtttc atcaggaaga gatgttgctg attttgaaat aataaaacat gcctgtgcca
    12661 aagatataat cataaccaat gattacggtc tggcggcaat ggctcttgcc aaaaggactt
    12721 atgtgctgag tttttcaggc ttactctatg atgaaagcaa tatagattct cttcttatgc
    12781 aaagacacat aggcatgaaa gaacgaagaa acggaaacta taccaagggt cctaaaaaaa
    12841 gaaccaaaca ggatgatctg aattttgacg ctgctctgaa ccggcttatc gaaaaacaaa
    12901 taagtcagga ataatcatat ttttttaaaa agtattcgtg aagagtttag taccgcaata
    12961 agagctactc ccacatcggc aaatatagca tgccacatgt ttgccagccc gaatgtgctt
    13021 aagacgataa ccagaacttt tacagcaatc gaaaagaata tgttggtaat agccgttttc
    13081 tgggtaatcc gggctatttt tactgcagtt ataatgcttg ccggattatc gttcattata
    13141 actatgtctg atgcctgaac ggctgcctgg cttgcggctt tccccataga tattgatata
    13201 tcggccttcg ccattaccgg ggagtcgttt atgccgtcgc ccacaaatac aacgctgtgg
    13261 tttttctttt ttctcagcag tatcttatcc agtacttcaa gtttttgtcc gggaagaagc
    13321 ctggaaaaaa cattctcttt tttgattcca agttgtgagg caacctcaag tgcactttct
    13381 gtcttatcac cggtgagtat ataggtttca atcttctgat ctgcaaaaaa ctctatggtt
    13441 tgaacggtct gtggtttgac agagtcactg aaggagatct ttccagcata atttcctgaa
    13501 atacctacat atatgcaggt tctgtcatcg ttttttccgg taaactccgc atccgaaact
    13561 gcaatgcttt cctttaggta ctcgtatctt ccgcatacca gctcaatacc ttcatatgta
    13621 gcttttatac cttttcccat tacttcttca tacttttcaa tcttattttc atctattgtt
    13681 ccataatctt ttgtgtattt cttcattacc gaaactgcaa cggggtgtga agaatgaact
    13741 tcacatacag ccgcataaaa aagcaatgat tggggagtgt atccctctgc cggtataatt
    13801 gacgttattt caagctctcc gcttgtaagg gttcctgttt tatcaaaaag aacgctgtca
    13861 attgcattta gtccctcaag gtaattagtt cctttcagca gtataccttt tttggaggcc
    13921 acacccgctc cggcaaagta tgccagcggt accgagagaa caagtgcgca gggacaggaa
    13981 acaacaagaa atataagtgc cctgtaaagc cattctgaaa aaggctgtcc taaaaatacc
    14041 ggaggaataa ctgctgtcaa taatgccgct aggagaaccg caggggtata gtatcttgca
    14101 aacttagtta taaactgttc tgttttggac ttcttttcag cgctattttg aaccatctct
    14161 attatttttg ataccgttga ctcagtaaat ggtcttgaag cttttactgt aataagagag
    14221 ctctgattta catacccgga aagaacctcg tcaccacgga ctatgccaac aggcatatac
    14281 tcaccggtta tagcgctcgt atcagcataa gaacttccgt gtacgataac tccgtcaagc
    14341 ggtatttttt ccccgggctt taccacgaac tcatcacccg ttcgtatagt ttgaggagaa
    14401 acctgtacgg ttttaccgtc cttaagtatg ttaacatact ctggtttaag atcaagaaga
    14461 gcctttatgg agtttttagt attgtttacc gccatatcct gaaaaaattc gccgatctta
    14521 taaaaaatca ttacagctac cgcttctgga tactctttta ccgcaaatgc ccccaaagat
    14581 gccacagtca taagaaaatt ttcattgaaa atgttcttgg ctgaaactcc cttataggcc
    14641 tttatgatta tatcccatcc ggttataatg tatgcgcccg catatagaaa tacgcttata
    14701 tatggagagt tgcccataag aatggcaaag aggaaaaccg ctactcctgc tgcaaaggtg
    14761 tatacttcct tttttgaaag tttaccgggt tcgtctccgg atttttcagc tgaattagac
    14821 gtggtttcga ctacttttac atcaggttcc aattttctta ttattgagtt ggttttgagc
    14881 attatagccg cgatattttc ctgcccagat acttctatac taagggtttt ggtcataaag
    14941 ttcagagttg ctttgtcaac tccttctaat ttatttacac gttcttcaat ctttcttgca
    15001 cagttggcac agtcaagtcc ctgaagtgta acttcttttt ttaatttttt cataggacgc
    15061 ctactcttca agatgattta tggcctggtc aaatatcttt ctaacatgct cgtcggccat
    15121 agagtagtaa actgcttttc cttctttacg gaactttatg agtcttgcct gtttaagtat
    15181 tctcagctga tgtgacacgg cagactgctt catttcaaga acgcttgcaa gatcacatac
    15241 acacatttcg ctcacgaaaa gagcatcaag aatctttact cttgtactgt caccgaaaac
    15301 cttaaaaagc tctgccatat catagataat ttcatcatca gacatttctt tacggacttt
    15361 ttccacaata tctttgtgtt ctgaaacaca gtcacatgaa ggaattttct cttcgtactc
    15421 cataattcac ctcttcttat ctgcaaagta ttcagtagat tatatgaaca tatgaatata
    15481 tattcatatg aatgataact catttcccaa taaatgtcaa gcaaaaaaat tgcagatact
    15541 taagtatctg caataaaatt aaaatgaatt tttcaggtag taggtgttgc aactgcgctt
    15601 acagtcagtt taaacataga atccaaaaat ggatcgaaaa gtattttgga ccattcctgg
    15661 gttgttgtgt aatcatgtcc gtccatttta atgtcaatct tatctattgc atagcccgga
    15721 tctgtttcca cgtggattac tatgtactca tagttgttgt acggaaacca gcttattttt
    15781 ttaattccct tgtttttatt ctggccgtag gagtaactgg aaagccacca ttgggcataa
    15841 acggttaaat cttcttcaat tttggtcatt gcgtctacgg tgtatcccga accatcggct
    15901 ttggtatccc atgattcaaa gctcaagcgt cctcttacag gcggctgggg aaggtttata
    15961 ataggctgcc cgctgtgaac ctttaatgtt tggggaacag cctgggttga accgccgttg
    16021 ctgttgaagg taacagtccg aagatcttta tcattgggca tacaggaaga cagtacaaat
    16081 aataagatca aagagataac ggtaaaaagt aacttttttt caaaggtatt ccctccttaa
    16141 aagagttttc ttatacgatt atacactata caatgtaaaa aaataaaaat tgcagatgcc
    16201 atagcatctg caattcatct atatcagatt ctgcttaaga atccatcata ttctttatct
    16261 gtacctatga aatcgaatat cagggttcct tccctggaaa gatttatggc agtttccaag
    16321 tcaattctct catgaaaaag atcaaaataa tactcattgg tcttttcatc acgttttagc
    16381 tgcgcataat ttaggctttc cattgcaata ctgtccgaaa acgcatcatc ctcgttcgtt
    16441 ctgtgaagag cataaaacgc attttcctga agataaaagt cgtcgtaata ctccagaggg
    16501 tctatcaaga caaaccactt cctttatttt tataagatat atcttacctt atatcatatt
    16561 tatgtaaaat ttgcaagtgg ctgtctcata ccttatgtta aattatacgt tgtcaattat
    16621 ccaagcactc ttttgaccga agtaaagatt tatgggttga ttcagcacag ggagttgatt
    16681 ttccatggag ttgtacgaaa ggacatgaat gttttgtcct ccgcaaagcc ttacatcgta
    16741 ttcaaaatgc gaaccgagaa agacatactt gtctatcttt ccgcaaagca tattttcacg
    16801 gccttctttt tctaggttta ttgcctgggg acggaggatg atgttagcgt cgtctccttc
    16861 tttaaatgct tttttggtta tgccgtaaaa ggtctggttc agttctttac tgaaaacttt
    16921 tatctgtgaa ccttcctgtg cgataacctt ggcttttata atgttgacgg ttcctataaa
    16981 atcggcaaca aacttgtttt cagggtatgc gtacacttca aagggtgaac ctacctgcat
    17041 tatctttccc ttgtccatga tgactacctt gtcagaaatg ctcatggctt ctgtctggtc
    17101 atgagtaacg tatatactgg tgatattaag ctctttttgg attctgcgta tttcaagccg
    17161 catattttca cgcagtttag cgtcaaggtt ggaaagaggt tcgtcaaaaa gcaggacttt
    17221 cggttccata actatacacc tggcaagagc cactctctgc tgctgtcctc ctgatatctg
    17281 atcgggtctg cgcttttcca ttcccttaag tccagtaagt tctattatct tgtcggcttt
    17341 ttgtttgact gtgtttttag ggagtttttt aattctaagt
//
LOCUS       DAOSHR010000022        16686 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_2813_length_16686_cov_6.103361, whole genome shotgun sequence.
ACCESSION   DAOSHR010000022 DAOSHR010000000
VERSION     DAOSHR010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 16686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16686
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2813_length_16686_cov_6.103361"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="PLU28_02180"
     CDS             <1..373
                     /locus_tag="PLU28_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG33906.1"
                     /translation="SGKGIQSENYYSNFRQKMYEYKDVFNSIQFTDKDIAILKSKDLS
                     EFIYYFIAYMGEKKIGKKFEDGINFDKFNTHFNSFFSLILKENNDYCKSGIIEQVLKE
                     KSVLVLQVFEEYRKYFVEDMK"
     gene            370..1701
                     /locus_tag="PLU28_02185"
     CDS             370..1701
                     /locus_tag="PLU28_02185"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOG33907.1"
                     /translation="MIYIKDKEYIKKFMESLNRQFREKGENIQPEDFFTQDYEKASCY
                     IVDESEFEKPKDSRQIEEFKSYFPMIIIVEAKICCEVMEIFLKYNVIGVFRKDRLEYE
                     YEEMGNFEKLLKEIIRNQSEELDTKCEERFVKVLGYKKISNWKLEIEDPIDTYKSKKY
                     ISIFYDKSMKDFSVRLRTIINDIKKCRKENTDQFGEIDKKIKNSKTRIDLSNFSDLVL
                     PSLLLEGETGTGKSMIAEVISKETGAGMYKFSLVNIEKNLVDTELFGSRKGAYTDAED
                     KPGRILKNAGKIIFLDEIAEVSLGVQTKLLLYLDDFKVRPIGSDETGMPAPAFIVAAT
                     NKNLKESIKDGSFRSDLYYRFSYRISIPPLRERKSDIRFLINFILQNPKINPYFEGKY
                     AVEKISIDAIEKLEEYNYPGNFREMEFIIQNAVNNAISERKDILLSEHLEF"
     gene            1810..2061
                     /locus_tag="PLU28_02190"
     CDS             1810..2061
                     /locus_tag="PLU28_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG33908.1"
                     /translation="MRISKNALRKAIRHGLKEKEILFILQNGKSRRVTRGALLTYIPE
                     NKIEDNKYLTKYSGIYVIHDEETVIEIFRNKDFRIKNIA"
     gene            2223..2936
                     /gene="cas6"
                     /locus_tag="PLU28_02195"
     CDS             2223..2936
                     /gene="cas6"
                     /locus_tag="PLU28_02195"
                     /inference="COORDINATES: protein motif:HMM:TIGR01877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HOG33909.1"
                     /translation="MRIKINLKGKNIDLPIHYNYAIQGLLYNIITQKFKDVHDIGEVY
                     ENQKFKPFCFSRIFSSKYEIKDKRILFDGEIYFIFSSPFSDISDHVVNYFLNNNTVRL
                     GDNIVSVSSLKSNDYKLKNSMKVRALCPITVYETQKNEDSKFVRFFSPDMVEFKECIK
                     SNMNKKAAILGIRSGTFDISPFKVTEKDEKVIRYKQSIIKGWTGIYSISGSDEMMRIA
                     FEWGLGVKNSEGFGMVEEV"
     gene            2984..3628
                     /locus_tag="PLU28_02200"
     CDS             2984..3628
                     /locus_tag="PLU28_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG33910.1"
                     /translation="MKKLLGILLMVLLVSLAFSSSQEATPTTVPEPQTQQATSTEVLE
                     TQVLDVQEQAVPSLEELLNRKFEIQQELISLEKYLNDYLKTSVSSVAEMTDFIRTIEE
                     LIKTINEKMISLDEEIDIKVNEIQIKLTDFESNITPVIESYKNEIEILKTEMESLKKE
                     MESLKNENTQLKARLDSIESSIKSLQSSSSRPGIWDFIIIALCIAAAAAGILIK"
     gene            3688..4335
                     /locus_tag="PLU28_02205"
     CDS             3688..4335
                     /locus_tag="PLU28_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HOG33911.1"
                     /translation="MKKDVRKYTLGEEIANSITHGIGGLLSIAGLVILIIFAAIYGTA
                     WHVVSFTIFGVSLVVLYTMSTLYHSIQHEKAKHIFQILDHSSIFVLIAGTYTAFTLTT
                     LRGPLGWTIFGIIWGLAALGITLSALYIKKYKAIAALAYIGMGWIVIMAIKPLWDNLP
                     RISFIFLLIGGLCYTVGAVFYMMKKVKFMHAVWHLFVLGGSIFHFFSAIYSLPKL"
     gene            4475..6229
                     /locus_tag="PLU28_02210"
     CDS             4475..6229
                     /locus_tag="PLU28_02210"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HOG33912.1"
                     /translation="MSIVGPRVWILLVSSLLMFLLNIFSLGKGERGIAHPFSASMACA
                     SLWSLGFFFEIVCISFTDKVFWYNLQYLPMFLLPVFWFWTTLKITGVFPFFKRKHLII
                     FAGLSIIFFVLVATNSFHGMLLTNLGTITEDGYTFILGTPTPLFYWISIYEYSFLVIS
                     FFMVVIKVFSSKGMYVVIYLLLLSALLFPLVFNTLHLLQVIKYSFNYTPFFFLISGVI
                     MAYSIVKLKIYNIVPIAKDLLVSNMADGLIVIDKAGYIIEFNNRACEIFSMHTNDVLG
                     KSAEKVFPSIKEYFNSRGELDDEWNFFWKNRYYNASTRKVSRNGKVISKIFVVKDIDE
                     NIRYEKQILESRERAERINYEKGVFLQAMSERIRNNIDDIFAYLDQIMDTPLSETQID
                     YISKSRTSSEFLYEIINSLTDVSKIESGSIELNNQNFNPVKMLGDIKDLFMPIVRGKN
                     LELKIQFFNHEQGSYSGDVVKIKQILVNLLNNSIKFTTEGGVYLNIIHGRLYGNMRKV
                     VFVIKDTGIGMSSEQMERLFSPFSQADSSISRKYGGSGLGLTIAKAFIERMSGHIYVR
                     SKIDAGSTFIFYLMVNAA"
     gene            complement(6235..6606)
                     /locus_tag="PLU28_02215"
     CDS             complement(6235..6606)
                     /locus_tag="PLU28_02215"
                     /inference="COORDINATES: protein motif:HMM:NF022158.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HOG33913.1"
                     /translation="MVFDVLSYGITAAGILVFILIFALVFFNVILGVFKSRHSKKEPL
                     ITVPAKVVKTLQDTSVQFSQIKPVRSKATVNNYYITFEFESTDRKVFEVSAHEYAQIT
                     EGDKGRLSFRGDVFIAFERLI"
     gene            complement(6617..7567)
                     /locus_tag="PLU28_02220"
     CDS             complement(6617..7567)
                     /locus_tag="PLU28_02220"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG33914.1"
                     /translation="MSYIQVEGLTKKYTIISKKTKKEIYALDNLFLQVKKGEFMGYIG
                     PNGAGKSTTIKVLTGIMVPSSGMVSVDGLCPWKDRKEHVRKIGAVFGQKTQMWWDLPV
                     IDTYNLLRDIFRISKTKYTQQLSYILENLDLQELLEKPVRFLSLGQRMRAEIGACLIH
                     DPDILFLDEPTIGLDIFSKEKVNNFLKELNKQGKTIFLTTHDLEDIQNLCSQIMILNN
                     GQIIFKGSKNELKAEAGLPLQVKVKIRKESIGQDNKKFLYLDNRSFNQEKYELTVMLH
                     GDEKPGDIAGTLFKLFEIEDIIIKEPDIEDVVKYFYKQDA"
     gene            complement(7578..8348)
                     /locus_tag="PLU28_02225"
     CDS             complement(7578..8348)
                     /locus_tag="PLU28_02225"
                     /inference="COORDINATES: protein motif:HMM:NF017946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HOG33915.1"
                     /translation="MIFSTLFKASMKTQFADRTNFFVDIFASGLSILSDFILIVFLLG
                     SMRTIKGWTLAELAFIYGITEGGWGFFRLFGEGLHNFEDLIVTGKFDTFLLRPVSPII
                     QIMLQKIDFKRAGIIIQAVAVGLWGVYNIESDFSLVFWFFLLMFFSVIMTFEINIFLA
                     SIAFWTTRNKDIIVLAFYSTKNASYYPADIYNRLIRGIITFIIPVATTGYYPLSFLTG
                     KSGNVFYLASPAIACTLLILPCAIIWNTGLKKYTGSGT"
     gene            complement(8350..9120)
                     /locus_tag="PLU28_02230"
     CDS             complement(8350..9120)
                     /locus_tag="PLU28_02230"
                     /inference="COORDINATES: protein motif:HMM:NF017946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HOG33916.1"
                     /translation="MSLFGLSKTAFKRNRIYVFNHMINTLGSFIFGYVYSCVWTALLH
                     DKPPSQVRQMVTYVMVNQASLWVTMFLPYGCFLYEKTREGTISFDLLKPYSLLQLSLF
                     EVGGHCFYNLLFRSLPIFLFSIFLLQIELPVPKILLPYFLSLFLAFLVSFFLNYIVGL
                     WALKFFNISAAQNTYYFLMNLFGGFFLPAEYFPPALRLVISIQPFACSGYIPSSIYLG
                     RLNPVFGITVQAVWVFVLWAVCRIITEKVTKDIRITGG"
     gene            complement(9237..9680)
                     /locus_tag="PLU28_02235"
     CDS             complement(9237..9680)
                     /locus_tag="PLU28_02235"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOG33917.1"
                     /translation="MTQQDLLKSALKTRKQRMTAKRELILRVFMDSNGKLLSIDDVFM
                     EMRKKRSHRTSKMTVKRAIDLLVDLGLLKEVLFDDDNIKYEYVKTQTNSGNAIFVCTK
                     CGKAFNVDLDQKMLKELFKNQSFHVKEVSLKISGICEECAEILSK"
     gene            complement(9989..11014)
                     /gene="nusA"
                     /locus_tag="PLU28_02240"
     CDS             complement(9989..11014)
                     /gene="nusA"
                     /locus_tag="PLU28_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOG33918.1"
                     /translation="MNINLLEALDAFEKEKGISKDALINIIQKSITSAYKKNYGTKNV
                     EVLLDKSLTALNVFQIWKITETVSDPNEEISLEDALKMDSKAVLGGEIRKKVNPKKDF
                     NRIATQTAKQVIMQNIKELEKNTLFEKYVPLKDKITNAEVSKVSPDGADIRIGKLETK
                     LPAKDMIPNESMKQGDLVKVYIKDVQYTSKGPRIIVSRTVPEFIKELLKTIVPEIEED
                     IVSIVRIYREPGIRTKIAVSSKDPKVDPVGSCIGENSSRISELINEVKGEKVDIIRYS
                     DDPSEFIKNSLAPAEVLSITLNPDKKEAVVTVPESQFSLAIGKGGQTARAAAKLTGWK
                     IDIHTPA"
     gene            complement(11047..11511)
                     /gene="rimP"
                     /locus_tag="PLU28_02245"
     CDS             complement(11047..11511)
                     /gene="rimP"
                     /locus_tag="PLU28_02245"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HOG33919.1"
                     /translation="MKDLKKDIFQAAQSFAQELGLIIFDIVITQKKKKTTLEIIIDSL
                     ESYIGIDECESFSKKIDPWLEENDPFEMKYDLIVSSPGLDRPLRDINECLRFKGRLAK
                     FILKEPLENRTVLKGYIQEVQNDTVSILESDSKKVFTFDFSKVKKANLEIDL"
     gene            11696..13108
                     /locus_tag="PLU28_02250"
     CDS             11696..13108
                     /locus_tag="PLU28_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG33920.1"
                     /translation="MKKLVFTVILMSLFAVMSFSQDVAFLVRKEDGKVMDQYKVSLEE
                     AMGEYYNNLLDIMQSDPSYDTFLNMPQNLYYTIGSLADFLIIQYYLDEKGIVVNMDSV
                     MSQTKELYDAYMTNDMYRDYLTSVFENENNFYSYVRGIFVKDALIGSLRNQFTDISKD
                     RVLNYVTNNFNNLVLELSTESETYETPESMMESPTIVEKMVFDIATNDLVEWFSIYRT
                     KFDFAVNYKPLELEEKLQKTVDMNEKIQLEEEYRGKIMTAEKIPQEWLISYSMLADQV
                     ITDKKAILQEINVYAQLKTVYPEESKMTPEQIDSLVAGIVEEQSTAASTEVSEKLMQK
                     ENDLRNLAYLVYYYNGLSDSQIQALKADSENKISEIEAFHVTALKNLYRFNPDNGNLV
                     YKIHELDPDDPVIAYQYFESFYSMLVQYIDDPVNRDIVILKLTEIKQQAEVIASSIKD
                     NAELQALYTELIKEIDSLIK"
     gene            13156..16032
                     /locus_tag="PLU28_02255"
     CDS             13156..16032
                     /locus_tag="PLU28_02255"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOG33921.1"
                     /translation="MKKFLKTIDHALLTITDDLFEKAVQSYETVMVSDIKFETANGIL
                     KIKGRVEDGSDAFDTLVSISEEKIIYRVCMCSNKEIELCVHQVLLLLKAKKIILKDEC
                     GENDYIKSVLDSLQTQARQGHADAYGLFEDAAEGKVYIKPLKKSKKEYMPIEMEMYDF
                     FCSAAGDSRENDIKSFTYPDIKTGKLFLRAFRSMREKMTEIFYDNKIISFGSDVNLYC
                     TIDKNSNNNYGLRFELKSDGESMSAEKVLSSLQDCVVMDEKKQLRIVDVNYPFDISKL
                     VLQTVQNIPYDEITNDYIHKLMGTGIKFFSNTPDIKVLQGPQFYELYIFRIDQTFKFN
                     LKISCEGVSYTFRQAGQMIRDTVCEQEVVSSLLRAGVKLDKNGELSMSPSKFVKFLNE
                     GILEIDRRIRVLYTQDIKVISTPRVTVSGTRNTDWFELNICIEDQNIKQYYEEIKKSG
                     YIVLKDGGIVEVPPKVRKLVESIERNGGKVPFYKLFDFKQEADGEVKEYLERFDRFES
                     VSSDAFVSKLPLREYQSKAVDYLRFLYGYGINAILADDMGLGKTVQTIALLGGKKSSK
                     KDLILCPKSVLYNWAAEIDKFSDMSWHIYGQGKNICPDTRIIIATYSALRNSDELQSK
                     EYNFIVLDEAQYIKNSWTQTSETVKSLSAEHKLALTGTPIENSIMDIYSIFDFLMPDF
                     LGEEKKFLQTCVQQEGKNHIASKIKPFVIRRTKEEVLKDLPQKNIQSVYVSMEEKQEE
                     IYADLLAQARYKLKYINNDSEIKFSALELLLRLRQAACHPKLLFQWYQNGSGKFEEFK
                     SITNTVIQSGHKILVFSQFVSMLDIMERYLDEAKIKYFAITGSTENRAEVCEKFNNSN
                     NTRILLCSLRAGGTGLNITGADYVLHYDPWWNRAVENQATDRAYRIGQTKNVFVYRFI
                     TKNSVEEKIEKLQNSKQEISNAFVSENNILQNLTKQEITELFED"
     gene            16052..>16686
                     /locus_tag="PLU28_02260"
     CDS             16052..>16686
                     /locus_tag="PLU28_02260"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOG33922.1"
                     /translation="MSLLILNLNPGYDRWIIFSKEPELKKVQRGDKVVSYCAGKGLNM
                     SRVLKSLGYEDYRCINIIGGHVGSIIRLKSTEEGLNPVFWGISDESRVNTALVYENRK
                     EMIMINEPGPFMSRDEVESFKEFFNTTLKKEHEVVLSGSAPRGFEAEDIFQMLHSAKK
                     SGAKVYVDIAGRWLEKAALTSPDLIKVNNDEFINAFGIDGGDIPKVSAFRK"
BASE COUNT         5484 a         2740 c         3327 g         5135 t
ORIGIN      
        1 atccggaaaa ggcattcagt ctgaaaatta ctattctaac ttcaggcaaa agatgtatga
       61 gtataaggat gttttcaaca gcatacaatt tactgataaa gatatagcaa tcctcaaatc
      121 aaaagatctc tcggaattta tctattattt tattgcatac atgggtgaga agaaaattgg
      181 taaaaagttt gaagatggta ttaattttga taagtttaat acgcatttta actcgttctt
      241 ttctcttata cttaaagaaa acaatgatta ttgtaaaagc ggaataattg agcaggttct
      301 aaaagaaaaa tcggtacttg ttcttcaggt atttgaagag tataggaaat atttcgtgga
      361 ggatatgaaa tgatatatat aaaagacaaa gagtacataa aaaagtttat ggagtctttg
      421 aacagacagt ttagagaaaa aggagaaaat attcagccag aagatttttt tacgcaggat
      481 tacgaaaaag caagctgtta tattgttgat gaaagcgaat ttgaaaagcc taaggatagt
      541 agacagatag aagagtttaa atcgtatttt cccatgatta ttattgtaga ggctaagata
      601 tgctgcgagg ttatggaaat ttttttaaag tacaatgtaa tcggtgtttt cagaaaagat
      661 agacttgagt atgagtatga ggaaatggga aactttgaaa agcttttaaa agaaataata
      721 agaaaccaga gtgaagagtt ggataccaag tgtgaagaac gatttgtcaa agtcttaggc
      781 tataagaaga taagcaactg gaagctcgag atagaagatc ccattgatac ttacaagtca
      841 aaaaagtata taagcatatt ttacgataag agcatgaaag atttttcggt cagactaagg
      901 actataataa atgacataaa aaagtgccgt aaagagaata cggaccagtt tggagagata
      961 gacaaaaaga ttaagaactc aaaaaccaga atagatcttt ccaattttag tgatcttgtt
     1021 ctaccttcgc ttttgttaga aggagagacg gggacaggaa agtctatgat tgcagaggtt
     1081 atatccaaag aaacaggagc agggatgtat aagttttcac ttgttaatat agaaaaaaat
     1141 cttgtggaca ctgagctttt cggaagcagg aaaggtgctt atacagatgc tgaagataaa
     1201 ccgggtagaa tactcaaaaa tgccggaaag attatatttt tggatgaaat agccgaagtt
     1261 agcctggggg tacaaacaaa actgctttta tacctggatg atttcaaggt tcgtcctatc
     1321 ggttctgatg agaccggaat gcctgctccg gcttttatag ttgcagctac taacaagaac
     1381 ttaaaagaaa gcataaaaga cggaagtttc agatcagatc tgtattacag gtttagttac
     1441 aggataagca ttccgccttt aagggagaga aaaagcgata tcagatttct tataaacttt
     1501 atacttcaaa atcctaaaat aaatccatac tttgagggaa agtatgcagt ggaaaaaata
     1561 tcaatagacg caatagaaaa acttgaggag tacaactatc ccggaaattt cagggagatg
     1621 gagtttataa tacaaaacgc tgtaaataat gcaatttctg agagaaaaga tattttgctt
     1681 tcggagcatt tggagtttta agatgaccgt tgatggtaat atgtaaggtt ttgaaaaagc
     1741 tctccggaga tatttaactg taaatgtcga ataataattt gaagacaatg aaggagggga
     1801 gggcatagta tgcgtatatc aaaaaacgct ctaagaaagg ctatcagaca cggactgaaa
     1861 gaaaaagaga tccttttcat tcttcaaaac ggcaaatcca ggagagtaac cagaggagca
     1921 ttactaacgt atatccctga aaataaaata gaagacaaca agtatcttac aaagtattcg
     1981 ggtatatacg ttattcacga tgaagaaacg gtaatagaaa tattcagaaa caaggacttc
     2041 agaatcaaaa atattgcata atacaaaaga agactgcggt atgagcagtc ttcttttttt
     2101 ttgattaaaa gataaaagga tatattaatt caaaaaaatt ttctaaaaga agtatgatat
     2161 aatataacag aaacataaaa acacgtagac tatgaaaaat agtcaggctt ggaggattag
     2221 cattgagaat aaagataaat cttaagggaa aaaacataga tcttcctatt cattacaatt
     2281 atgctataca aggtcttttg tacaacatta tcacacagaa gttcaaggat gttcatgata
     2341 taggtgaagt atatgaaaac caaaagttta aaccattttg tttttcaaga atattttctt
     2401 caaaatacga gatcaaagat aaaagaatac tttttgacgg agagatatac tttatttttt
     2461 catctccgtt ttctgatata tcggatcatg tagtaaatta ctttcttaat aataatacag
     2521 taagacttgg agataatatc gtcagtgtga gttctttgaa aagcaatgat tataagttaa
     2581 aaaacagtat gaaggtgaga gcgctttgtc ctataactgt ttatgaaact caaaaaaatg
     2641 aagactcgaa gtttgttaga tttttcagcc cggatatggt ggagttcaag gaatgtatca
     2701 aatcaaatat gaacaaaaaa gcggcaattt tgggtataag atcaggtact tttgatattt
     2761 ctccttttaa agtaaccgaa aaagatgaaa aagttataag gtataaacag tcaataataa
     2821 aaggctggac gggaatctac agtatatcag gcagcgatga gatgatgagg atagcttttg
     2881 aatggggttt gggggtaaaa aactccgaag ggttcggaat ggtggaagaa gtttaaaaat
     2941 attatataat aagaaggggt taaaatttat aagaggtgat agtatgaaaa aacttttggg
     3001 tattttgctt atggttcttt tagtctcgct tgcattttcg tcttcccagg aagctactcc
     3061 tactacagtt ccggagcctc agactcagca agctacttct acggaagttt tggagacaca
     3121 ggttttagat gttcaggaac aagctgttcc gtctcttgaa gagcttctta accgaaagtt
     3181 tgagattcag caggagctta tctctcttga gaagtatctg aatgattatt taaaaacttc
     3241 ggtttcaagt gttgccgaga tgactgactt tattaggact atagaggaat tgataaaaac
     3301 gattaatgaa aagatgatca gcctggacga ggagatagat attaaggtca atgagattca
     3361 aataaagcta actgactttg aatcaaacat tacccctgtt attgaaagct ataaaaacga
     3421 aattgaaata ttgaaaacag aaatggagag tctgaaaaaa gaaatggaaa gtctaaaaaa
     3481 cgagaatacg cagttaaaag caagacttga ctctattgaa agcagtatta agtctcttca
     3541 gagcagttcg tccagacccg gaatatggga ttttataatt atcgcactct gtatcgcagc
     3601 ggcagcagca ggaatattaa taaaataaag gtataatagg atatttggaa gtaaagttgt
     3661 gatgcctttc aagagggagg tgaaaacatg aaaaaagacg taagaaagta tactttgggt
     3721 gaagagatcg caaacagtat tacgcatggt atagggggac tgctaagtat tgccggatta
     3781 gtcatcctta taatatttgc cgctatttac ggaacagcat ggcatgttgt cagttttacg
     3841 attttcgggg tatcccttgt agtgctttat actatgtcta cgttatatca tagcattcag
     3901 catgaaaagg ccaagcatat ttttcagatt cttgatcact catctatatt cgttcttata
     3961 gccggaacct atactgcctt tactcttact actctgagag gccccctggg atggacgata
     4021 ttcggtataa tatggggtct agctgcatta ggcattactc tttctgcttt atatataaaa
     4081 aagtataaag cgatcgctgc acttgcttac ataggtatgg ggtggatagt cataatggct
     4141 attaaaccat tatgggacaa ccttcccagg ataagcttta tctttttact tatcgggggc
     4201 ctttgttata ctgtcggagc tgtattttat atgatgaaga aggtgaagtt tatgcatgcc
     4261 gtatggcatc tcttcgttct gggaggaagc atatttcact ttttttcggc aatatacagt
     4321 ttacctaaac tatagtaaag atcagaatcg cgagcgtgag cccgcgattt ttttatgatc
     4381 ttaaaattca attctttttt tgtgcataca tatgttaaat gtttagtgta taatattaaa
     4441 tgttattaca aaaaaagttg agcggtgagt tattatgagc attgtaggtc ctagagtctg
     4501 gattcttctg gtaagttccc tattgatgtt tttattgaat atatttagcc ttggaaaagg
     4561 tgagagaggc attgcccatc cgttttccgc ctcaatggcc tgtgccagtc tgtggtcgct
     4621 gggctttttt ttcgagatag tctgtatcag ttttacggac aaagtttttt ggtataatct
     4681 tcagtatctt cccatgtttc ttcttcctgt tttttggttt tggacaactt tgaagattac
     4741 cggtgttttt ccctttttta agaggaagca tctgattatt tttgctggtt tatctataat
     4801 attttttgta ttggttgcaa ccaactcttt tcatgggatg ctccttacaa atctcggcac
     4861 aataaccgaa gatggatata ccttcatact gggaacacct acgcctcttt tttactggat
     4921 aagcatatat gagtactcct ttcttgtgat atcttttttc atggtagtta taaaagtatt
     4981 cagcagtaaa ggcatgtatg tggtgattta tcttttgctt ttgagtgcct tgctttttcc
     5041 tttagttttc aatactcttc atctgcttca ggtaataaag tacagtttta actacacacc
     5101 cttcttcttt ctcatttctg gggttataat ggcctattct atagtcaagc tgaaaatata
     5161 taatatcgtc cccatagcga aagatctcct tgtatccaat atggcagacg gcttgatagt
     5221 gatagataaa gcagggtata tcatcgagtt caacaatagg gcctgcgaaa tatttagcat
     5281 gcatacaaac gatgttttgg gaaaaagtgc agaaaaggtt tttccaagta taaaagagta
     5341 ctttaactct cgtggggagc ttgatgatga atggaatttt ttttggaaaa acaggtatta
     5401 caatgcttcc accagaaaag tatccagaaa cggaaaggtt atatccaaaa tttttgttgt
     5461 caaggatata gatgagaata taaggtatga aaagcagatt cttgagtcaa gggagagggc
     5521 tgaaagaata aattatgaaa aaggtgtctt tcttcaggct atgagtgaaa gaataagaaa
     5581 taatatagat gatatatttg catatcttga tcaaataatg gatacaccac tttctgaaac
     5641 gcagattgat tatatttcaa aatcaaggac ttcatcggag tttctttatg agataattaa
     5701 tagcctaact gatgtatcga agatagaaag cggaagtata gagcttaaca atcagaactt
     5761 caaccctgtg aagatgcttg gggatataaa ggatcttttc atgcctatag tcagaggaaa
     5821 gaatctggag ttaaaaatac agttttttaa tcatgaacag ggttcatact ctggtgatgt
     5881 ggtaaagata aaacagatac ttgtaaatct tttgaacaac tctataaagt ttaccacaga
     5941 aggcggtgtg tacttaaaca ttattcacgg cagattatat ggaaatatga ggaaagtagt
     6001 ttttgtgata aaagataccg gaattggaat gtcatccgag cagatggaaa gattgttttc
     6061 ccctttttct caggcggata gctccataag cagaaagtat ggaggctcag gtcttgggct
     6121 cacgattgcc aaagcattta tagaaaggat gtcgggtcat atttatgtac gcagtaagat
     6181 agatgccgga agtactttta tattctatct gatggttaat gcggcgtagg tttcctatat
     6241 cagcctttca aaggctataa atacatctcc cctgaatgaa agcctgccct tgtcaccttc
     6301 agttatttgg gcatactcat gtgcggaaac ttcaaatact tttctgtcag tactttcaaa
     6361 ttcaaaagtt atataataat tattgactgt agcttttgat cttacgggct ttatctgaga
     6421 aaattgaaca gatgtgtcct gaagggtttt aaccactttg gcgggaacgg ttatgagagg
     6481 ttcttttttg ctgtgccttg atttaaaaac gcctaaaatt acgttaaaaa agacaagtgc
     6541 gaatatcagg atgaatacta atatccccgc agcagttata ccgtaagaaa gaacgtcaaa
     6601 caccataaag ccctccttat gcatcttgtt tatagaaata ttttaccaca tcttcaatat
     6661 ctggttcttt tataattata tcttcaattt caaataattt aaaaagagta ccggcaatat
     6721 ctccgggttt ttcatcgcca tgaagcatta cagtcagttc atatttttcc tggttaaacg
     6781 aacggttatc cagataaagg aactttttgt tatcctgacc gatagattct ttgcgtatct
     6841 tcacttttac ctgaagcggc aatcctgctt cagccttcag ctcattctta cttcccttga
     6901 aaattatctg tccgttattt aaaatcatta tctgagaaca caaattttgt atatcctcaa
     6961 gatcatgcgt tgtaagaaag atggtcttac cttgtttatt gagctcttta agaaagttat
     7021 taactttttc ttttgaaaat atatccagcc ctatagttgg ttcatccaga aaaagaatat
     7081 ccggatcatg tatcagacat gcccctattt cagctctcat acgctgccca aggctcaaaa
     7141 accttaccgg tttttctaaa agctcctgaa gatcaaggtt ttcaagtata taagaaagct
     7201 gctgggtata cttggttttg gatattctga aaatatcgcg caggagatta taagtatcta
     7261 ttaccggcag atcccaccac atctgggttt tctgtccaaa aaccgctcca attttcctaa
     7321 cgtgttcctt gcggtctttc catggacaaa ggccgtcaac actgaccata ccggatgaag
     7381 gaaccattat tccggtgaga acttttattg tagtagattt tcctgctccg ttagggccta
     7441 tatagcccat gaattcgcct ttcttaacct gtaaaaacag attatccagg gcataaattt
     7501 ctttttttgt cttttttgag ataatagtat attttttagt gagcccttca acttggatat
     7561 aactcatgct ttcctcctta tgtcccgctc ccggtatatt ttttaagacc tgtattccat
     7621 attatggcac acggcagtat caaaagagtg caagctattg ccggagatgc cagatagaaa
     7681 acgtttccac tttttcctgt cagaaacgaa agaggatagt atcccgtagt tgcaaccgga
     7741 ataataaaag tgatgatacc tctgatcagg cggttataga tgtccgccgg atagtacgaa
     7801 gcgttcttgg tcgaataaaa tgccagaact ataatatctt tatttcgggt tgtccaaaag
     7861 gctatacttg ctaggaaaat atttatttca aaagtcatga tcacagagaa aaacatcagt
     7921 aagaaaaacc agaaaaccag gctgaaatca ctttcaatgt tgtatactcc ccaaagacct
     7981 acagccacag cctgaataat tatgcctgct cttttgaaat ctattttctg aagcataatc
     8041 tgtattatag gactgacagg tcttaggagg aaggtatcaa actttccggt tactattaga
     8101 tcttcaaaat tgtgcagtcc ttccccgaaa agcctgaaaa atccccatcc tccttctgtt
     8161 attccgtaaa tgaaggcaag ttccgctaaa gtccatcctt ttatagttcg catacttccc
     8221 agtagaaata ctataagaat aaaatcgctt agtatgctta atccgcttgc aaatatatct
     8281 acaaaaaagt tagttctgtc tgcaaactga gttttcatac tggctttaaa aagagtagaa
     8341 aatatcatat caccctcctg taattctaat atctttggtg actttttcgg ttataatcct
     8401 gcagactgcc cagagcacaa aaacccagac ggcttgaacc gtaataccaa aaaccggatt
     8461 gagcctgcca aggtagatgc ttgaaggtat atatccgctg caggcgaagg gttggattga
     8521 tataaccaag cgtaaggcag gcggaaagta ttcagccgga aggaaaaaac cgccgaaaag
     8581 attcatcaaa aaatagtaag tattctgtgc ggcagagata ttaaaaaact tcaatgccca
     8641 aagaccgaca atataattca gaaaaaacga gaccaaaaaa gccagaaaaa gagataagaa
     8701 atacggaaga agaatttttg gaacaggcag ttctatttgg agaagaaaga tactgaaaag
     8761 aaatatcgga agacttcgga acaaaagatt gtaaaaacaa tggccgccta cttcaaaaag
     8821 acttaactgt aaaaggctgt aaggcttgag aagatcaaag gaaatggttc cttcccgtgt
     8881 tttctcatag aggaaacatc cgtaaggcag gaacatggtt acccaaaggg atgcctggtt
     8941 taccatgaca taagtaacca tctgtctgac ctgtgacgga ggcttatcgt gaagcagtgc
     9001 cgtccataca catgaatata cgtatccgaa aataaaactg ccaagagtgt ttatcatgtg
     9061 gttgaatacg tatattctgt ttcttttgaa agctgtttta gaaagtccga acaacgacaa
     9121 aattttgaac acctccttat gtgggtgttc attgaaaagc ggataaaggt attctaccat
     9181 atttcttcat tttttcaaaa taaaaaatgc ctgattataa atcaggcatt caatagttat
     9241 ttagaaagta tttcagcgca ttcctcacat attccggaga ttttcaatga aacttcttta
     9301 acatgaaaag actggttttt aaaaagttct ttaagcattt tctgatcaag atctacgtta
     9361 aatgcttttc cgcatttggt acatacaaat atggcgtttc cactgttggt ttgggtttta
     9421 acgtattcat atttgatatt atcatcatcg aaaagcactt ctttaagaag tccgagatca
     9481 accagaaggt ctatagcacg cttaacagtc atcttgcttg ttctgtgact ccttttcttt
     9541 ctcatttcca taaaaacatc gtcgatgctt agcagtttcc cgttagaatc cataaagact
     9601 ctcagaataa gttccctttt agcagtcata cgttgctttc tagttttcag ggcgcttttg
     9661 agtaaatcct gttgtgtcaa tcacatcgcc tcctaatagt attttagttt atattttaaa
     9721 cgatgatatc atatgattat taacataatg tttatattac acttaaaata ctactattag
     9781 gttaaaacat ggcacatcta aatttttatg ccggaacttt aatttttttt tgtcgaaagc
     9841 tttagaattt ttaagccgtt agcttttgaa ttgttaagcc gctagcttta gatcttttaa
     9901 gccgctagct tttgaatttt taagccgcta gctttagatc ttttaagccg taagcttttg
     9961 aattgttaag ccgctagctt tagatctttt aagccggtgt atggatatca atcttccagc
    10021 cggtgagctt tgcggcagct cttgccgtct gtccgccctt tcctattgca agggagaact
    10081 gggattcagg aactgtaaca acagcttctt ttttatcagg attaagggta attgaaagca
    10141 cctcagctgg tgcaagagaa tttttaataa attctgatgg atcgtctgaa taccttatta
    10201 tatcaacttt ttcaccctta acttcattta tgagctcaga tattcttgaa ctgttttcac
    10261 cgatgcagga gcctaccgga tcaaccttgg gatctttgga tgagactgct atcttggttc
    10321 ttattcccgg ttctcggtat atcctcacta tactgactat atcttcctct atttcaggaa
    10381 ctatagtttt aagcagttcc tttataaatt caggaacagt acgtgaaact attattcttg
    10441 ggccttttga tgtatactga acatctttga tgtatacctt aacaagatca ccctgcttca
    10501 tagactcgtt aggtatcata tccttggccg ggagcttggt ttcaagcttt cctatcctta
    10561 tatctgcccc gtcaggagat actttagata cttcagcatt tgttatctta tcctttaacg
    10621 gaacatattt ttcaaaaaga gtatttttct caagctcttt tatattctgc attattacct
    10681 gttttgctgt ctgagtggca attctgttaa agtccttttt aggattgact tttttacgta
    10741 tttcgcctcc caaaacagct tttgaatcca tttttaaagc atcttccaaa gatatttctt
    10801 catttggatc gcttacagtc tctgttattt tccatatctg aaaaacgtta agggcagtga
    10861 gacttttatc taaaagcact tccacgtttt tggttccgta gttctttttg taggcacttg
    10921 ttatggattt ttggattata ttgataagcg catccttact tattcctttt tctttttcaa
    10981 aagcatccag agcctcaagc aggttgatat tcacagttcc acctccatca cttttatttt
    11041 aaataattat aagtctattt cgagattagc tttttttacc ttggaaaaat caaatgtgaa
    11101 tacttttttg ctgtcggatt caaggatact tacggtatcg ttctgaacct cttgaatata
    11161 acccttgagt acggttctat tttctaaagg ttctttaagg ataaatttgg ccagcctgcc
    11221 tttaaacctt aaacattcgt ttatatctct taatggtctg tcaagcccgg gagaactcac
    11281 aattaggtcg tacttcattt caaaaggatc attttcctca agccaaggat caattttttt
    11341 ggagaagctc tcgcactcgt ctattcctat atagctttca aggctgtcta ttattatttc
    11401 aagtgtggtt ttcttttttt tctgagttat tacaatatca aaaattatca gtcccaactc
    11461 ctgtgcaaag gactgagcag cctggaaaat atctttctta agatctttca tttcatattc
    11521 ctccggttta ctctaaaata aacttcgagg gcggaaaaat aggccctcga caaagagtaa
    11581 gtttatatat tattagttgc attatagcat aaatagattt aaattgcaag catattctaa
    11641 gttttttatg gtaaaattaa taacgtaatt gaaattttta taatgggagg aacatatgaa
    11701 aaaacttgta tttacagtta ttcttatgtc gttgtttgca gtaatgtcct tttcccagga
    11761 tgttgctttt ttggtaagga aggaagacgg taaggtaatg gatcagtata aagttagtct
    11821 ggaagaggct atgggggagt actataataa tcttttggat ataatgcaga gcgatcccag
    11881 ttatgacaca tttttaaata tgcctcagaa tctttattat accataggca gtcttgcaga
    11941 ttttctcata atacagtact atcttgatga aaagggtatt gtagtgaata tggactcggt
    12001 tatgagtcag accaaagaac tctacgatgc atatatgaca aacgatatgt atagggatta
    12061 cctgaccagc gtttttgaaa atgagaataa tttttattct tatgtcaggg gtatatttgt
    12121 gaaggatgct cttataggtt ctttaagaaa tcagtttact gatatttcaa aagacagggt
    12181 ccttaattac gttactaata acttcaacaa ccttgttctt gagttatcga cagagtcaga
    12241 aacctatgag acacctgaat caatgatgga aagtcctaca atagtagaaa aaatggtttt
    12301 tgacattgct acaaatgacc ttgttgaatg gttcagcatc tatagaacca agttcgattt
    12361 tgcagtgaat tacaaaccgc ttgaacttga agaaaagctt cagaaaacag ttgatatgaa
    12421 cgaaaagata cagcttgaag aggagtacag aggaaaaata atgactgcgg aaaaaattcc
    12481 tcaggagtgg cttatttcat actctatgct tgccgatcag gtaataacag ataaaaaagc
    12541 tattctgcaa gagataaacg tatacgctca gctcaagaca gtctatcctg aagaatcaaa
    12601 gatgactcct gagcagattg attccttagt ggccggtatc gtagaggaac agtccacagc
    12661 tgcctcaacc gaagtaagtg aaaagcttat gcagaaagag aacgatctta gaaatcttgc
    12721 atatctggta tactactaca acggactttc ggattcgcag atacaggcac tgaaagcaga
    12781 cagtgaaaat aaaataagtg aaatagaagc ttttcatgta actgctctga aaaatcttta
    12841 caggtttaac cctgacaacg gaaaccttgt atataagata catgagctgg atccggatga
    12901 tcctgtcata gcttatcagt actttgaaag tttttattcc atgcttgtac agtatataga
    12961 tgatcctgtc aacagagata tagtaattct aaagcttaca gagataaagc agcaggctga
    13021 ggttatagct tcatctatta aagataatgc agaactgcaa gccctatata cggagcttat
    13081 aaaggaaatt gacagtctaa taaaataaaa atatctatcc ccgcaagggg ataggttttt
    13141 gttggtgaaa cttatatgaa aaaattttta aagacaattg atcatgcact tcttaccatt
    13201 acagacgatc tttttgaaaa ggctgttcag tcgtatgaaa cagttatggt atctgatata
    13261 aagtttgaaa cagcaaacgg tatacttaag ataaaaggta gagttgaaga cggttcagat
    13321 gcttttgata ctttggtttc aataagtgaa gaaaagatca tatacagggt atgtatgtgc
    13381 agtaataagg aaatagaact ttgtgttcat caggtacttc tgcttttaaa agccaagaag
    13441 attattctta aggatgagtg tggggaaaac gattatataa aaagtgttct ggacagtctg
    13501 cagacgcagg ccagacaagg acatgccgac gcttatggtc tttttgagga tgcggcagaa
    13561 ggaaaagtat atataaaacc attgaaaaaa agtaaaaaag agtacatgcc tatagagatg
    13621 gagatgtatg attttttttg ttctgctgcc ggcgatagca gagaaaatga cataaaatct
    13681 tttacatatc cggacataaa aaccgggaag ctctttctta gagctttcag gagcatgcgt
    13741 gaaaagatga cagaaatttt ttatgataac aaaataataa gcttcggatc tgatgtaaat
    13801 ctttactgta ccattgacaa aaatagtaat aacaactatg gtcttcgttt tgaacttaaa
    13861 tctgatggcg aaagtatgtc ggcagaaaaa gtgcttagtt cacttcagga ttgtgtggtg
    13921 atggatgaaa aaaaacagct ccgaatagtt gatgtcaact atccgtttga tatttcaaag
    13981 cttgtcttac agacagtcca aaacatacca tacgatgaaa ttacaaatga ttatattcat
    14041 aagctgatgg gaacaggcat aaagtttttt tcaaatactc cagatataaa agtgcttcag
    14101 ggaccacagt tttatgagct ttacatattc agaatcgacc agactttcaa attcaattta
    14161 aagataagct gtgaaggagt ttcatataca ttcagacagg cagggcagat gataagggat
    14221 actgtttgcg agcaggaagt ggtttcaagc cttttaagag ccggtgtaaa gcttgataaa
    14281 aacggagaac tgtctatgag cccgtctaag tttgtaaagt ttcttaatga aggcattctt
    14341 gaaattgaca gaaggatcag ggttctttat acacaggata taaaggttat atcaactccc
    14401 agagtcacag tatcaggtac aagaaataca gattggtttg aacttaatat ctgtattgaa
    14461 gatcagaata taaaacagta ctatgaggaa atcaaaaaat ccgggtatat tgtactcaag
    14521 gacgggggaa ttgtcgaggt tcctccaaaa gtaagaaaac tggtcgaatc aatagaacgt
    14581 aacggaggaa aggttccctt ttacaagctt tttgacttta aacaggaagc tgatggtgag
    14641 gtaaaggagt accttgaaag gtttgacaga tttgaatcag tttcttccga tgctttcgta
    14701 tcaaaacttc ctctgaggga gtatcagagt aaagccgtcg attatcttag gttcttatat
    14761 ggctatggaa ttaacgcaat acttgcagat gatatgggat tgggcaaaac agtccagact
    14821 atagcgctgc ttggggggaa aaagagttca aaaaaagatc tgatactctg tcccaaatca
    14881 gttttatata actgggctgc cgaaatagat aagttttcag atatgagctg gcatatatac
    14941 ggtcaaggga aaaatatctg tcccgatacc aggatcataa ttgcaaccta cagtgcttta
    15001 agaaacagtg acgagcttca aagcaaagag tataatttta ttgtgcttga tgaagcacag
    15061 tatataaaaa acagttggac tcagacctca gaaactgtaa aaagtctatc tgccgagcat
    15121 aagttagctt taaccggaac tcccatagaa aacagcataa tggacatata cagtatcttt
    15181 gattttctta tgccggattt tttaggtgaa gaaaaaaaat ttctacagac atgtgtgcag
    15241 caggaaggca agaaccatat tgcttcaaag ataaaaccgt ttgtgataag aagaaccaaa
    15301 gaagaggttc ttaaggatct gccccaaaaa aacattcaga gtgtctacgt cagcatggag
    15361 gagaaacagg aagaaattta tgccgatctt cttgctcagg ccagatataa actcaagtat
    15421 ataaataatg attctgaaat aaaattttct gctcttgagc ttcttctaag actccggcaa
    15481 gcggcgtgcc atcctaagct tctttttcag tggtatcaga acggttccgg aaagtttgaa
    15541 gagtttaaaa gtattaccaa tacggttata caaagcggtc ataaaatttt ggtgttctcg
    15601 caatttgtaa gcatgctgga tattatggag agataccttg atgaggcaaa gataaaatat
    15661 tttgccataa ccgggagtac cgaaaatcgg gcggaggtat gtgagaagtt caataacagc
    15721 aataacaccc gcatactgct gtgcagtctc agggcaggcg ggacaggtct taacataaca
    15781 ggtgccgatt atgttttaca ttatgatccc tggtggaacc gtgccgtgga aaatcaggcc
    15841 actgacaggg cttatcgcat agggcagaca aaaaatgttt ttgtatatag atttataacc
    15901 aaaaacagcg ttgaagaaaa aattgaaaag cttcaaaaca gcaagcaaga gatttcaaat
    15961 gctttcgtgt cggaaaacaa tattcttcaa aatctaacga agcaagagat aacagaactt
    16021 tttgaagatt aataaaaaag gagtaaatag tatgagtctg ctgattctga atcttaaccc
    16081 tgggtatgac aggtggataa tattcagcaa agagccggag ctaaaaaaag tccagcgcgg
    16141 agataaggtg gtatcgtact gtgcgggcaa gggactgaat atgtcaaggg tattgaaatc
    16201 tcttggatac gaggattatc gttgcataaa cataataggc ggccatgtgg gaagtataat
    16261 aaggctaaaa agtacagagg aaggacttaa tcctgtcttt tggggcattt cagatgaaag
    16321 cagggtaaat accgctctgg tttatgaaaa ccgcaaggaa atgattatga taaatgaacc
    16381 tggtccattt atgagcagag atgaggtgga aagcttcaaa gagtttttca acaccacact
    16441 taagaaggaa cacgaggttg ttctttcagg cagtgcgccc agaggatttg aggccgaaga
    16501 tatctttcag atgctgcaca gcgctaaaaa atccggagct aaggtctatg tggatattgc
    16561 cggcagatgg cttgaaaaag ctgcgctgac atctccggat ttgatcaagg tcaacaatga
    16621 tgagtttata aatgcttttg gaattgatgg gggcgatatc cccaaagtaa gtgcttttcg
    16681 taaaga
//
LOCUS       DAOSHR010000023        16539 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_2851_length_16539_cov_5.508796, whole genome shotgun sequence.
ACCESSION   DAOSHR010000023 DAOSHR010000000
VERSION     DAOSHR010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 16539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16539
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2851_length_16539_cov_5.508796"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..1061)
                     /locus_tag="PLU28_02265"
     CDS             complement(36..1061)
                     /locus_tag="PLU28_02265"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOG33923.1"
                     /translation="MNDIIRLDRNESPFDLPADIKEEFFNELKKINFNRYPDPFSEGI
                     RKTIGNFINVPFENIAVGNGSDELIGNLLRVLEGEEVIITPPTFEMYYFYCSLNKVKI
                     IESELDSNWMISPEIFKLINKKTKAVFICSPNNPTGNIQDIKTITAILDTGVNVILDE
                     AYFEFSTQNCLYLTKKYRNLIILRTFSKAFGVAGVRTGYAIANSTVIEKIYKVRPPYS
                     LNSLSQSMIKVLLDNRELIQTRIQYIVRQRETMIEKLKEYAIPSQTNFIFLDFDRLNR
                     KDTFALSLYEFLYSEKFAIRTFKDKYVNKLRITLADKLINDELTEKILFFLKNRNSKI
                     TIVSHRE"
     gene            complement(1058..1690)
                     /gene="hisIE"
                     /locus_tag="PLU28_02270"
     CDS             complement(1058..1690)
                     /gene="hisIE"
                     /locus_tag="PLU28_02270"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HOG33924.1"
                     /translation="MSDYRISEEALDWKKFNGIIPVIIQNEEKEVLTLGWVDPKALTL
                     SLETGLMHYYSNREKRIRLKGEVSGNFQYIKNIAADCDNDSLLITVRQKGPACHTGTK
                     SCFKSLYTLPEVNSVPDYSLKVLKELEEIILERKNTYSQDSYTCSLFKKGKEEIYKKF
                     GEEAVEVLVSSTKERLEYEIADMIYHLLVLMRYEGIELKSIMAELKRRRK"
     gene            complement(1677..2429)
                     /locus_tag="PLU28_02275"
     CDS             complement(1677..2429)
                     /locus_tag="PLU28_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase cyclase
                     subunit"
                     /protein_id="HOG33925.1"
                     /translation="MLCKRIVVALDVKNGQVVKGVNFDNLSENGNPLELAVKYQQNGA
                     DEIIFLDISACLEKRKTLLQLAEKVSEKLSIPFTVGGGVSSVSDATELFKNGADKVFI
                     NSAALNNPELIKKLAMKCGSANIIVAVDSRLQNSEYKVYSSAGKKQTPYELAEWCLKA
                     QELGAGELMITSIDRDGTRKGFDLDMLGRLTDKISIPIIISGGAGSMNDFKKAFENGA
                     DAALGAGVFHSNTFTPNTLKLFLKEEKINVRL"
     gene            complement(2419..3114)
                     /locus_tag="PLU28_02280"
     CDS             complement(2419..3114)
                     /locus_tag="PLU28_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HOG33926.1"
                     /translation="MIFIPAIDLLDNQVVRLEKGSIDHVSCYGEPLEIAAKLSKRFKL
                     LHIIDLNGSFEGTPKNMELVKKIIQHTGVQIQLGGGLRSYRLIEEAFGIGVTSVILGT
                     KAFDTKFMEDVVKDFKNITVSLDIKDGKILLNGWTERKDISLAQAYSSLTPMIKRFIV
                     TITQNDGTLKGVQEIRKFWKEDEQVMYAGGIKGIRDLQKLQEIGFYGAVCGKAFYENL
                     ITPEILPEEFYAL"
     gene            complement(3111..3704)
                     /gene="hisH"
                     /locus_tag="PLU28_02285"
     CDS             complement(3111..3704)
                     /gene="hisH"
                     /locus_tag="PLU28_02285"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HOG33927.1"
                     /translation="MHKILIIDTGTGNLKNAAKAFGAEVASCEREIRRADKLVFPGVG
                     TFDTVMRNIHHLKDAILEHIQRQKPFFGICIGMQILFERSEEGCKKGLGIFKGTVVRI
                     KNAKTPHMGWNNLIIARDSRILKNLSEKDFFYFVHSFYAEPKNIETSAFAQHFSEYGC
                     INICSVIEENNIFLTQFHPEKSYLSGEKIIDNFRRIL"
     gene            complement(3704..4234)
                     /locus_tag="PLU28_02290"
     CDS             complement(3704..4234)
                     /locus_tag="PLU28_02290"
                     /inference="COORDINATES: protein motif:HMM:NF012687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase"
                     /protein_id="HOG33928.1"
                     /translation="MIRITSETKITVEDCTDMTIDCKDKIFEHMINTLFFYMNKKVSF
                     QACYDLRHHLWEDGGITTGMYLKELIKDKNYARYGTSIIPMDDALVLCCVDISRAYCE
                     IDIKIKDTEEGFETFLLTEFVGGLCRSLSVTVHLKQLSGYSGHHIIEACFKSLGLSLQ
                     QAIKDSSTVFSTKGVL"
     gene            complement(4231..5349)
                     /gene="hisD"
                     /locus_tag="PLU28_02295"
     CDS             complement(4231..5349)
                     /gene="hisD"
                     /locus_tag="PLU28_02295"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HOG33929.1"
                     /translation="MYDYVQSILEDIKENGMKALLKYSERFDGFSGPFAVSPQEMLQA
                     SINPQDEAVIDEIIQRVKSYHSRQEIKSIFYVHEDSEYGLLANPIERIGIYIPSGRSV
                     LVSTLIMTAIPAILAGVSEIAVTCGSAGGAVNPFILYTAKKLGIHELYKISGVQAIAD
                     MAYGINMRKTEKIFGPGNAYVTAAKKYLYGTVGIDCLAGPSEVCVIADDSADKNYVLN
                     DLQAQLEHGEDSRAYLITTSQDIFHSTTNPRILKVLCENIEECIKKVNEIAPEHLELM
                     VQNPEKYLAYIKNAGAVYCGDYSPTAASDYFSGSNHVLPTDKTARFSSVLTVYDFIKF
                     SSYVKMSQKEFLEYRRLGIRLAEIEKMPAHRKSLEVRQ"
     gene            complement(5321..5959)
                     /gene="hisG"
                     /locus_tag="PLU28_02300"
     CDS             complement(5321..5959)
                     /gene="hisG"
                     /locus_tag="PLU28_02300"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HOG33930.1"
                     /translation="MKLTFAVPSGRLSEKIRFFLEQADILVPVPVSRELSIQQDNITF
                     IYPRSSDIPVFVENGADMGICGSDTVAEQMPEIYTPVFLPVGACRMSIISHNFFDFSV
                     NDMKGFTVATKYPEITQRFFREREIPVKIIKLGGSVELGCISGISDCIVDIVDSGKTI
                     RENNLKEVLKIMDIQSVLCVNKLSMKVKNREITRIINKIKEASYVRLCPKHT"
     gene            complement(5956..6768)
                     /locus_tag="PLU28_02305"
     CDS             complement(5956..6768)
                     /locus_tag="PLU28_02305"
                     /inference="COORDINATES: protein motif:HMM:NF024785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HOG33931.1"
                     /translation="MNGTVLKRKDFLYVSEKYAFITSVIRDVLHSNGFHEIFPSSVHK
                     CTSSVCDGLKFSDGKNFYVINPDITSCLINSITESTAKIFYIAEKLDSYMNSSWQIGF
                     EIISPQESSNITDTLKTAITIMEKLGKTRFFIDLGSLKIWEDLCMGVKEFQKEIFEAV
                     KSQNFSSILEMSIPEKTKKSLLKTIELRTQNPPLPSIQEIMTEINDERLWFDASFINQ
                     KEYYTDIFFNLFSTDPTLMLGVGGTYKTKISWGCGMSFSLENLFRLIGGDIL"
     gene            7144..8493
                     /locus_tag="PLU28_02310"
     CDS             7144..8493
                     /locus_tag="PLU28_02310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOG33932.1"
                     /translation="MEGFYKRAFKLALPMALQQFISISVNFVDTLMIGQLGEIPIAAV
                     GLSNKVFFLYSLLLFGLCSGGSIFIAQFWGKKDFKNISKSMSIMAVSSVIFSSVFFVM
                     GAFFPHFTMRIFSPDIQVINQGAVYLFIIAFSIPFTGLTNVYEATLKSTEHPVLPMAA
                     SLIALSGNIFLNWVFIFGNLGSPKLGVQGAAIATLISRIVQFLILMLASEIRKYPARF
                     RMGDIKAISLNYLKAFFSYTIPTIGNEFLWSLGTVMYSIVYAHMSTQTIAARNVMETV
                     ESFAFALLFALAGATAIMIGKELGQSNYENARMTAKKMIVFTVMISTFTLSLVLIFAK
                     PMLGLFQVDDTVRKMIFNTLIISMVFVPIKTINLVGVVGILRSGGDTKFAFCTDVGAM
                     WIIGVPMAFLAGILLKLPFEIVYLFTMGEEVFKSFVLFFRLRSGKWIKNVVDAMQEI"
     gene            8496..9356
                     /locus_tag="PLU28_02315"
     CDS             8496..9356
                     /locus_tag="PLU28_02315"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOG33933.1"
                     /translation="MTKNKFLYAFLSAFLFGLSTPLSKIMLSEVPPVLLGGMLYFGSG
                     AGLAFVSLFGLRKKKRFDAADLKWLILSIICGGIIAPVVLLFGLNHTYSGTSSLLLNF
                     EAVATTVIAVLFFKEKANKKIFLSLFLITVACIILTVNFSEKIAFNSWSLLIVLACLM
                     WGMDNNFSKKMSENDPVTIVMIKGISAGAVNIILSLIFEKPTIQTSYIAFSAIIGFFC
                     YGVSIVLFILSLRSIGTSRTSMIFSSAPFFGALVSFVVFRTWPDTIFVLAFSILAFGI
                     LILQKADFQK"
     gene            complement(9383..10402)
                     /locus_tag="PLU28_02320"
     CDS             complement(9383..10402)
                     /locus_tag="PLU28_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG33934.1"
                     /translation="MKKLLLFFFILELCVAFSSVRQDFLYFSQDGTFNGKLSLSLYEY
                     KSYYMLFKEHESSFNFNINFGQKTTLQASACQWNCFPPQTADEYIYGGIQTVFTQSIF
                     GDHYNESFFMDLQNFLEVNSYLKKSTAFSPEQLYFDGAFHTGSVFSYNDKKTIFFNSS
                     FGLSGGIGTKTSLVPWYYLIFSLDSNGQMGLLQRYLILSGKLKYSMINDFSTDVPLYI
                     RPSYKVGENQCAVYGKNAFSAELELKTFFDFEYFFPMNVGWGNGLKLGSVWHDSVFEK
                     DMAYDLSLRSYLFYSFKEPWMDVVMNLKAGIITDIAHKPFESIMPFLNVNLEGNFLFL
                     LFALI"
     gene            complement(10399..12129)
                     /locus_tag="PLU28_02325"
     CDS             complement(10399..12129)
                     /locus_tag="PLU28_02325"
                     /inference="COORDINATES: protein motif:HMM:NF024151.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG33935.1"
                     /translation="MNKLKFIALLIVVSILLISCSAVSIDAAKNYMGRLPDPVKETVL
                     KKPYEKGSLSVGKFFYAGGVNPRRKEYSKETITVSTSVLDISKLVEKIDSGYWGYDCS
                     SVPLNGIVWYPKEKGRYPLVLCIHGNHPAQEFSEAGYDYLGEHLASLGIIFVSVDENF
                     LNGSIGNENDARAYVLLHHAKTILSWNNDPSNPLYGILDTQNIGIIGHSRGGEAVVHA
                     ASFNKLSRYPDNGTLEFDFNLPIRAVVSIAPIEGQYKPSMKTISTNNINYLVLHGSQD
                     GDVFFFAGTRLYNSVQLSEEFIKASFWIYGANHAQFNSIWAEKQDPFPSLRSCLLSVE
                     QQQKTAKTLITSFLLSSFKIEEGYREFFKTPRAYHEWMADTFYINSFAGGNERILADY
                     NEDIELASVGLKNWEASAKYFENWSESKIWLISGDQNNWGTYLKWDTLSKVSPIPSYV
                     LSSKDSSIKTDELAFDIFVYNVQDTNSATSSKSENAVLEMLNFTIRLTGSDEKQQVYS
                     LREFLDIGKAPAVTVLNVREDIRTVLQSVYIKLKESMDIQKIEFIFDKSKKGDILIDN
                     IRYTEGGKSK"
     gene            12304..12879
                     /locus_tag="PLU28_02330"
     CDS             12304..12879
                     /locus_tag="PLU28_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HOG33936.1"
                     /translation="MQFVRDFLGIFSSIAQDLHMYLLKDSSVHSKLQIFLTSPSFHGL
                     MAYRFYSFFNRYRFYILSWPMYFWARIIFSMDIHPSAEIQPGVVIDHGIGVVIGETAS
                     VGTGSLIYHGVTLGAKNNCLGKRHPQVGNNVVIGAGAKILGYVFVGNDVKIGANSVVL
                     RDIPSMVTAAGIPAEIKKNKAVYYAQKEVEK"
     gene            12876..13766
                     /gene="cysK"
                     /locus_tag="PLU28_02335"
     CDS             12876..13766
                     /gene="cysK"
                     /locus_tag="PLU28_02335"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HOG33937.1"
                     /translation="MIPDSIGGTGLVTLSKIIDPFRIFVKLEKNNRTGSIKDRPALFM
                     INDAEKRGFLGKNKKTIVEPTSGNTGIALSAIGISRGYRVILTMPQNMSRERIELLKL
                     YGADVVLTPDEKGMRGAIEKALEIVESKNAYMPDQFSNPANPFSHEITTGPEILKQAD
                     YRVDAFVAAVGTGGTITGVGRALKKQLGKTVKIVAVEPLQSAVLSGNKAAAHKIQGIG
                     AGFIPKNYELSVVDRVITVDQEEVFEILKKIITYEGLSIGLSSAANILAALKLSGELG
                     GKSRITTVACDGVEKYMSLI"
     regulatory      14540..14711
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            14842..15816
                     /locus_tag="PLU28_02340"
     CDS             14842..15816
                     /locus_tag="PLU28_02340"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOG33938.1"
                     /translation="MTIGIVGASGEVGRTMIKVLEKMEVSIGELILLASSKSAGKELI
                     YKDKRIKIQELTEDKMKEKYDYLLFSAGGDVSRKYAPLAQEYGNTVIDNSSAFRMQKD
                     IPLVVPEINASLLKGYTGIVANPNCSTIQMALALNRIHENAGLKEVVVSTYQAVSGAG
                     NKGISELQAQQSGDMEIKHFPDQILNNVIPKIGEYEPNGFTQEEMKMVNETRKIFDDK
                     SIDVFATTVRVPVFYGHSESVFFRTKKELQLSEVKKLIETSENVVYSDEIITPVHIAQ
                     SDLTYVCRLRKAGPKEYMMWVVADNVRVGAATNAVRILLSHALLNAGK"
     gene            15820..>16539
                     /locus_tag="PLU28_02345"
     CDS             15820..>16539
                     /locus_tag="PLU28_02345"
                     /inference="COORDINATES: protein motif:HMM:NF014803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HOG33939.1"
                     /translation="MPKKTVPFTIEDIKALTEKYPTPFYIYDEKAIRDNIRVFLKEFS
                     WIDFKEYFAVKATPNPSIMKILSDEGCGMDCSSMPELVLAESIGCSGRQIMFTSNDTP
                     KQEYEKAFSLGAVINLDDYSHIKSIEHMISPDSSMCLRYNPGNRRGNEIIGQLKDSKF
                     GMSREQIFKSVYELKKYGIKSIGLHTMICSNELDPKYFQITAKNMFELAFDVSSEFDI
                     KVGFVNLGGGIGVAYREDQPQM"
BASE COUNT         4942 a         3001 c         3237 g         5359 t
ORIGIN      
        1 gaacttttta catacgaagc ggtttattaa aagttctatt ctctgtggct tacaatagta
       61 atttttgagt tgcggttttt aagaaaaaaa agtatttttt cagttaactc atcgttgata
      121 agtttatcag ctaatgtaat tcgcagtttg ttaacatatt tgtccttaaa agttcgtata
      181 gcgaattttt ctgaatacaa aaattcataa agacttaatg caaaagtatc ttttcggttc
      241 aacctgtcaa agtccagaaa gatgaagttg gtttgacttg ggatggcata ttctttaagt
      301 ttttcaatca tggtctctct ctgtcgtact atatactgta tacgggtttg tatgagttca
      361 cggttatcta aaagtacttt tatcatactc tggctaaggc tatttaagct gtaagggggt
      421 ctgaccttat agattttttc tatgacggta ctgttggcaa tggcataccc tgttcttact
      481 cctgcaactc cgaatgcttt tgaaaaggtt cgaagaataa taaggtttct atactttttt
      541 gtaagatata agcagttttg ggttgaaaat tcaaagtatg cttcatcaag tattacattt
      601 acgcctgtat ccagaatagc ggttatggtc tttatatcct gaatatttcc ggttggattg
      661 tttggtgagc atataaagac agctttggtt tttttgttta tcagtttaaa tatttccggt
      721 gatatcatcc agttactgtc aagctctgac tctatgatct ttactttgtt aaggctgcag
      781 taaaagtaat acatttcaaa tgtaggggga gtgattataa cttcttcccc ttcaagaacc
      841 cttagcagat taccaataag ctcatcactg ccatttccta cagcgatatt ctcaaaggga
      901 acgtttataa aatttccgat tgtttttctt ataccttcag aaaatggatc cgggtatctg
      961 ttgaagttaa tttttttcag ttcgttgaag aactcctctt tgatatcggc cggaagatca
     1021 aatgggcttt cattccggtc aagtcttata atatcgttca ttttctcctc cttttcaatt
     1081 ctgccatgat gctttttaat tcgatccctt cgtacctcat aagcaccaga agatgataaa
     1141 tcatatcggc tatctcgtat tcaagacgtt cctttgttga acttacaagg acttctacgg
     1201 cctcttcccc aaacttttta tagatctcct cttttccttt tttaaataag gagcatgtat
     1261 aagagtcctg actgtaagta ttttttcttt ccaaaataat ttcttcaagc tctttaagga
     1321 ctttaagaga gtaatcaggt acagagttga cttcaggtag agtgtatagg cttttgaaac
     1381 agctttttgt gcctgtatgg caggctgggc ctttttgtct gactgttata agcagggagt
     1441 cattgtcaca gtctgcagcg atatttttta tatattgaaa gtttccgctg acttcccctt
     1501 tcagacgtat tcttttttct ctgttgctgt aataatgcat taatcctgtt tctaaagaaa
     1561 gagtaagagc ctttggatcg acccatccca aggttaatac ttctttttct tcattttgga
     1621 taattacagg gattatcccg ttaaattttt tccagtccag agcttcttca gatattctat
     1681 aatctgacat ttatcttctc ctccttaaga aataatttga gagtatttgg tgtaaatgta
     1741 ttgctgtgaa atactcctgc tcccagggca gcatccgcac cgttttcaaa agctttttta
     1801 aagtcgttca tgctacctgc tcctcccgat attattatag gaatagagat cttatcagta
     1861 agtctgccga gcatgtcaag gtcaaaacct tttctagtac cgtctctgtc aatcgaggtt
     1921 atcataagct ctcctgctcc gagctcctgt gctttgagac accattctgc cagttcatat
     1981 ggggtctgtt ttttacctgc gcttgagtaa actttgtact cagaattttg taacctactg
     2041 tcaactgcga ctataatgtt cgcgctgcca catttcatgg ccagtttttt tataagctca
     2101 ggattattaa gagcagcgct gtttataaat actttgtccg caccattttt aaaaagctcc
     2161 gtcgcatcac ttacagaaga tactcctccc ccaactgtaa aaggaataga aagcttttcc
     2221 gatacttttt ccgcaagctg caaaagagtt ttcctttttt cgagacaggc ggaaatatca
     2281 agaaatataa tttcatcagc tccgttttgc tgatatttga cggcaagctc taaagggtta
     2341 ccgttttcag atagattgtc aaagtttacc ccctttacga cctgtccgtt ctttacgtca
     2401 agtgcaacta ctattctttt acagagcata aaactcctcc ggcagtattt caggagtgat
     2461 aaggttttca tagaatgctt ttccgcagac agcaccgtaa aatccgattt cctgaagttt
     2521 ttgtaagtct cgtattcctt ttatacctcc tgcatacatg acctgttcgt cttctttcca
     2581 gaactttctg atttcttgta ctccttttag agtgccgtca ttctgtgtaa tggttacgat
     2641 aaaccttttt atcataggag ttaaagagct gtacgcttgt gcaagcgaga tatcctttct
     2701 ctctgtccag ccattaagca gtatttttcc atctttaata tcaagactta ctgttatgtt
     2761 tttgaaatct tttactacat cttccatgaa tttagtatca aaagctttcg tgccaagtat
     2821 tacagaggtt actcctattc cgaaagcttc ctcgataagt ctgtagctgc gtagacctcc
     2881 tcccaactgt atttggacgc cggtatgctg tataattttt ttaaccagtt ccatattctt
     2941 tggagttcct tcaaaagacc cattaaggtc aattatgtga agcagtttga atctttttga
     3001 gagctttgcg gcaatttcca gtggttcacc ataacaagat acatggtcta tacttccttt
     3061 ttccagacgc acgacctgat tgtcgagaag atctattgca ggaataaata tcataatatc
     3121 ctcctgaaat tgtcaataat tttttctccg cttaagtaag atttttcagg gtgaaactgc
     3181 gtgagaaaga tattgttttc ctctatgacg ctgcaaatgt ttatacagcc atattctgaa
     3241 aagtgttgtg cgaatgctga tgtttcaata ttttttggtt cggcataaaa agaatgcaca
     3301 aaataaaaga aatctttctc agatagattt ttgagtattc ttgaatctct ggctattatc
     3361 aagttgttcc atcccatatg gggagtcttg gcgtttttta tccgtacaac agtacccttg
     3421 aaaattccca gacctttttt gcagccttcc tcgctccttt caaagagaat ctgcattcct
     3481 atacatattc cgaaaaaagg tttttgccgt tgtatatgtt ccagtatggc gtcttttaaa
     3541 tgatggatgt tcctcattac cgtgtcaaag gtgccaaccc cggggaaaac aagtttatct
     3601 gcccgtcgta tttccctttc gcatgaggct acttctgcac caaaagcttt ggcggcattt
     3661 ttaaggtttc cggttcctgt atcaataata agtattttat gcattacaat acccccttgg
     3721 ttgaaaaaac agtgctgcta tcttttatag cctgctgcaa agaaagaccc agtgatttaa
     3781 aacaggcttc aattatgtga tgaccactat acccagagag ctgttttaaa tggacggtta
     3841 ctgaaagcga ccgacagaga cctcctacaa attcagtaag gagaaatgtt tcaaatcctt
     3901 cctctgtgtc ttttatcttt atgtctattt cacaataagc tctagatata tcaacacagc
     3961 aaagcacaag tgcatcatcc ataggaatta tggaagttcc ataacgtgca taatttttgt
     4021 cttttataag ttctttcaga tacattccag ttgttatgcc tccatcttcc catagatggt
     4081 ggcgcaggtc ataacaggct tgaaaagata ctttcttatt catgtaaaaa aataaggtgt
     4141 ttatcatatg ctcaaaaatt ttatctttgc agtctatagt catatccgtg caatcttcca
     4201 cagttatttt ggtttccgaa gtgattcgta tcattgtctt acctccagtg attttctgtg
     4261 tgcgggcatc ttttctattt cagccaatct tattcctagg cgtctgtact caagaaactc
     4321 tttttggctc atcttaacat aagaagaaaa ttttataaag tcataaactg taagaacaga
     4381 tgagaacctg gcggttttat cggtcggaag aacatgattc gaaccgctga aataatcaga
     4441 agcagcggta ggagagtagt ctccgcagta gaccgcaccg gcgtttttta tatatgcaag
     4501 atatttttca gggttttgca ccataagttc aagatgttcc ggtgcaattt cgttgacttt
     4561 tttaatacat tcttcaatgt tttcacacag caccttcaat attctgggat ttgtagttga
     4621 gtggaagata tcctgtgaag ttgttataag ataggctctc gaatcttctc cgtgctcaag
     4681 ctgtgcttga agatcattca gcacataatt tttatctgca gaatcatcag ctataacaca
     4741 tacctctgaa ggacctgcaa gacagtctat tccgactgtt ccatagagat actttttagc
     4801 agccgtaacg taagcatttc ccggtccgaa aattttttcg gtttttctca tgtttatgcc
     4861 gtatgccatg tcagcaatag cctgtactcc gctaatttta taaagttcat gtattcctag
     4921 tttttttgct gtatatagta tgaaagggtt tactgctccg ccagctgaac cgcaggttac
     4981 agcaatttcg gatactccgg ccagtatggc aggaatggct gtcatgatga gcgttgaaac
     5041 cagaacagac cttccgctgg gaatatatat tcctatacgt tcgatgggat tagccaaaag
     5101 tccgtactca gagtcttcat ggacatagaa aatacttttg atttcctgtc tgctgtgata
     5161 actctttact ctttgaatga tctcatctat tacagcctca tcctgcggat ttatagacgc
     5221 ttgaagcatt tcctgcggac ttacagcaaa ggggcctgaa aagccgtcaa accgttcact
     5281 gtattttaaa agtgctttca taccgttttc cttaatatct tcaagtatgc tttggacata
     5341 atcgtacata agaggcctcc tttattttgt tgattattct ggttatttct ctgtttttaa
     5401 ctttcattga aagcttattt acacaaagaa ccgactgtat atccataatc ttgagtactt
     5461 ctttgagatt attttccctt atggtttttc cgctgtcgac aatatcgact atacagtcgc
     5521 ttataccgct tatacagcca agttcaaccg aaccgcccag ctttattatc ttaacgggaa
     5581 tttctctctc cctgaaaaac ctttgggtta tctccggata cttagtcgca actgtaaacc
     5641 ctttcatgtc attaacagag aaatcaaaaa aattatggga tattatgctc attctgcaag
     5701 ctccaacagg caggaaaaca ggagtatata tttcaggcat ctgttcggca acagtatcgc
     5761 ttccgcatat tcccatatca gctccgtttt ctacaaatac gggaatgtca gagcttcttg
     5821 ggtatatgaa agttatatta tcctgttgaa tggatagttc ccgtgaaaca ggtacaggca
     5881 ccagaatatc ggcttgttcc aaaaaaaatc tgattttttc ggaaagtctt ccgctcggaa
     5941 cggcaaatgt aagtttcaaa ggatatctcc tccgatcaac ctaaaaaggt tttctagact
     6001 gaacgacatg ccgcatcccc acgaaatttt ggttttgtaa gttcctccaa ctccaagcat
     6061 aagtgtggga tctgttgaaa aaagattgaa aaagatatct gtgtaatact ccttctgatt
     6121 aataaaggat gcatcaaacc aaagtctttc atcattaatc tctgtcataa tttcttggat
     6181 tgacggaagg ggagggttct gcgttcttaa ctctatggtt tttaagaggc tttttttggt
     6241 tttttctgga atactcattt ccagaattga tgagaagttt tgacttttca ctgcctcaaa
     6301 tatctccttt tgaaactctt ttactcccat acaaagatct tcccagattt ttaacgagcc
     6361 tagatcaatg aaaaacctgg ttttccctag cttttccata atagttatgg ctgtttttaa
     6421 agtgtctgtt atgtttgagc tctcctgtgg acttattatt tcaaagccta tctgccagga
     6481 tgagttcata tagctgtcaa gcttttctgc aatataaaat atcttggctg tactttctgt
     6541 aatactgttt atcagacagg atgtaatatc tggatttata acataaaaat ttttaccatc
     6601 tgaaaacttc agtccatcgc acacagagga tgtgcattta tgaaccgaag atgggaatat
     6661 ttcatgaaag ccgttgctat ggagtacatc tcttatcaca gatgttatga aagcatattt
     6721 ttccgatacg tataagaaat cctttcgctt taaaaccgtt ccgttcatgg tatcatctcc
     6781 cgtagaaaaa taaaaaaata ccggtcactg ttacgtgacc ggtattttgt attggatcag
     6841 tctttaagaa atacacaaaa agacatgatc tgataaaaac cgatcacgcc tcgtatggtg
     6901 catattcata ttaaaagttt tttgacagga tattgaagtc tttgacataa agctctcctt
     6961 tgaaaataag cttaacgtat tttaaggatt ataacataag aagatatcgc ctgtcaatgc
     7021 cagaagaata ctgttgataa aaagtcgtcc gaagttaata ggtgctttac gtaaataaag
     7081 accatgattt gttataatat tatctgtaaa gatatataaa gcttaaaaca aaggggtttt
     7141 agaatggaag gtttttacaa aagagcattt aaactggcgc ttcccatggc tcttcagcag
     7201 tttatatcca taagcgtcaa ttttgttgat acactaatga taggtcaact gggagagata
     7261 ccgatagctg cggtcggact ttccaataaa gtttttttcc tttactcgct tcttttattc
     7321 ggcttatgta gcggcggttc aatatttata gctcagttct ggggaaaaaa agattttaaa
     7381 aatatttcca agagcatgtc tataatggct gtaagctcgg tgattttttc ttctgtattc
     7441 tttgtaatgg gagcattttt cccacatttt acaatgagga tattttctcc ggatatccag
     7501 gttattaacc agggagctgt ttatcttttc ataatagctt tcagtattcc ttttacaggg
     7561 cttacgaacg tttatgaggc aacgcttaaa agtactgaac accctgtgct tcctatggcg
     7621 gcaagtctta ttgctctgag cggaaatatc tttcttaact gggtttttat attcggaaac
     7681 cttggttctc ccaagcttgg agttcagggt gcagcaatcg caactcttat atcaagaatt
     7741 gtacagtttc tgatacttat gcttgcaagc gaaatcagaa aatatcctgc cagatttcgc
     7801 atgggtgata taaaagcaat aagcttgaat tatcttaagg cttttttttc gtatactatt
     7861 ccgactatag gcaatgagtt tctctggtcg ctgggaacgg tcatgtactc tatagtctat
     7921 gctcatatga gtacccagac tattgcagca agaaacgtta tggagacagt cgagtccttt
     7981 gcctttgcac ttttgttcgc acttgccggg gcgacagcca taatgatagg aaaagagctg
     8041 ggacagtcta actatgaaaa tgccagaatg acggccaaaa agatgatagt gtttactgtt
     8101 atgatttcaa catttaccct ttccttggtt cttatatttg ccaaaccgat gcttgggctt
     8161 ttccaagttg atgatacagt aagaaagatg atcttcaata cgctcataat atctatggtt
     8221 tttgttccta taaaaactat aaatctggtt ggggttgtgg gtatcctcag atctggagga
     8281 gatactaagt tcgctttctg tacagacgta ggagcaatgt ggataatagg tgttccgatg
     8341 gcatttctgg cagggatact tcttaagctt ccgtttgaga tagtctatct ttttaccatg
     8401 ggagaagagg tttttaaatc ctttgttctt tttttcaggc tcagatcggg taaatggata
     8461 aaaaatgtgg ttgatgcaat gcaggagatc taaaaatgac aaaaaacaag tttttatatg
     8521 catttctttc ggcattttta tttggattaa gcactcctct ttctaaaata atgctttctg
     8581 aggttccacc ggtacttctg ggtggaatgc tttattttgg aagcggagcc ggtctggctt
     8641 ttgtatcttt gtttgggtta agaaaaaaga agaggtttga tgctgcagat cttaagtggc
     8701 ttattcttag tattatctgt gggggaatca ttgcacctgt agttcttctt ttcgggctaa
     8761 atcacacata ttctggcaca tcgtcacttc tgctgaactt tgaggctgtt gcgacgacag
     8821 ttattgctgt tttatttttt aaagagaaag ccaataaaaa aatttttctt tcactttttc
     8881 ttattactgt cgcatgcata atccttacgg taaatttttc agagaaaatc gcttttaatt
     8941 catggagctt actcatagta cttgcatgtc tgatgtgggg aatggataac aacttttcaa
     9001 aaaaaatgtc tgaaaacgat cctgtgacaa tagttatgat aaagggtatt tcagcgggag
     9061 ctgtcaatat tattctttct ctaatatttg aaaaacctac tatacaaaca agttatatag
     9121 ctttttcagc aataatcggt tttttctgct atggagtttc aatagtacta tttatacttt
     9181 cattaagatc tataggcacg agcaggacaa gcatgatttt ctcatccgca ccttttttcg
     9241 gtgctctggt ttcatttgtg gtctttagaa catggccgga tactatattt gttttagctt
     9301 tttcaatact tgcctttgga attcttatac ttcagaaagc tgattttcaa aaataaatat
     9361 cagggcttta aagccctgat atttatataa gtgcaaaaag cagaaaaagg aagtttcctt
     9421 caaggttgac atttaaaaaa ggcataatac tttcaaatgg cttatgggct atatcggtta
     9481 taattccagc ctttaggttc atgactacgt ccatccatgg ttctttgaaa gaataaaaaa
     9541 gataacttct taaagataga tcgtaagcca tatctttttc aaaaactgag tcatgccaga
     9601 ccgaaccaag ttttaaaccg ttgccccatc ctacgttcat aggaaagaag tattcaaagt
     9661 caaaaaaagt ttttagctcc agctccgccg aaaatgcatt tttgccataa acggcacatt
     9721 gattttctcc cactttatag cttggtctta tgtaaagagg gacatctgtt gaaaagtcgt
     9781 ttatcatact gtacttaagc tttcccgaaa gaataagata tctttgcaaa agacccatct
     9841 gaccgttgct gtcaagcgaa aaaataagat agtaccatgg tacaaggctg gttttggttc
     9901 caatcccccc tgaaagacca aaactgctgt taaaaaaaat tgtttttttg tcattataag
     9961 aaaacacaga acctgtatgg aaagctccat caaaataaag ctgctcagga gaaaaggcgg
    10021 tagatttttt caaataagag tttacctcaa gaaagttttg cagatccata aaaaaacttt
    10081 cattataatg atctccgaag atactctgag taaaaacagt ctgtatccct ccatagatat
    10141 attcatcggc tgtttgtgga ggaaagcagt tccattggca ggctgaagcc tgtaaggttg
    10201 ttttctgacc aaaatttata ttgaaattga atgagctctc gtgttcttta aaaagcatat
    10261 agtagctctt atattcatac aagctgagag aaagctttcc gttaaaagta ccgtcctgtg
    10321 aaaaataaag aaaatcctga cggactgacg agaaagcaac acataactca agtataaaaa
    10381 agaataggag taattttttc attttgactt ccctccttct gtgtaacgta tgttatctat
    10441 tagaatatct ccttttttgg acttatcaaa tataaactca attttctgga tgtccatgct
    10501 ctctttgagc tttatatata cactctgcag aacagttctt atatcttctc ggacattgag
    10561 aaccgtaacg gccggagctt ttccgatatc caaaaactct cttaaagaat aaacctgttg
    10621 tttttcgtca ctgccggtaa gccttatggt gaaattaagc atctcaagaa cagcattttc
    10681 tgatttggat gatgtagcgc tgtttgtatc ttgaacattg tagacaaata tgtcaaaggc
    10741 tagttcgtct gtttttatgg agctgtcctt tgaagaaaga acataactag gaatcggaga
    10801 gactttagat aatgtatccc atttcagata agttccccag ttgttctgat ctccagatat
    10861 aagccatatc ttggattctg accagttttc aaaatacttt gcggatgctt cccagttttt
    10921 aagtccgaca gaagcaagct ctatatcctc attataatct gctaaaattc tttcatttcc
    10981 tccggcaaaa gagtttatat aaaacgtatc agccatccac tcatggtagg cacggggtgt
    11041 tttgaagaac tcacggtatc cttcttcgat tttgaaactg gatagaagaa aagaagtaat
    11101 aagagttttg gctgttttct gctgctgttc cacagataaa agacagctcc taagggaagg
    11161 aaaaggatcc tgcttttctg cccatatgct gttgaactgt gcatgattgg caccgtaaat
    11221 ccagaatgaa gctttaataa actcttcgga tagttgcaca ctgttatata gccttgtccc
    11281 ggcaaagaaa aacacatctc cgtcctgcga accatgaagc acaaggtagt taatgttgtt
    11341 tgtagaaatt gtcttcatag aaggcttgta ctgtccttct attggagcta ttgatactac
    11401 agctctgatc gggaggttga aatcaaactc cagagtgccg ttatccggat atctggaaag
    11461 cttattgaag ctggctgcat ggactactgc ctcaccgcct cgtgaatgcc ctattatacc
    11521 tatattttgt gtatccaata ttccgtagag agggttggag ggatcattgt tccagctcaa
    11581 tatagtttta gcgtgatgga gaaggacata tgcccgggca tcgttttcat ttccgataga
    11641 accgttaaga aagttttcgt caacggaaac aaaaataata cctaatgaag ccagatgttc
    11701 tcccagataa tcgtatcctg cttctgaaaa ttcctgtgca ggatggtttc cgtgtatgca
    11761 aagcaccagg ggataccttc ccttttcttt gggataccat actattccgt tcagaggaac
    11821 acttgaacaa tcatatcccc aatatccgga atctatcttt tcaaccagtt tggaaatatc
    11881 caaaactgag gttgagaccg ttatagtttc cttggaatac tcttttcttc ttggattgac
    11941 accgcctgcg taaaaaaatt ttcctacact caagctacct ttttcatatg gttttttcag
    12001 tactgtttct tttaccggat cgggcagcct gcccatataa tttttggcag catctataga
    12061 aacagcactg caagaaataa gcagtataga tacaacgatc agcaatgcaa tgaatttaag
    12121 tttattcata tatcgtctct ccttcaaaaa gtttttacat gtgaattata gcatgtctgt
    12181 tgtgatatgc aaaaagctat agtattgaaa attatttgta aaaaagattt ttgtattttt
    12241 aatgattcaa gtatacaata ctttaaatat tgataaaaaa ggtggtgttt ttatggaact
    12301 gggatgcagt ttgtcagaga ttttttaggt attttttctt ccatagcgca agatcttcat
    12361 atgtatcttc tgaaagattc ctcagtgcat tcaaagctgc agattttttt gacctctcct
    12421 tcgtttcacg gactcatggc ttatcgcttt tacagttttt tcaacaggta cagattttat
    12481 atactatcct ggccgatgta cttctgggca aggatcattt tttccatgga tatccatcct
    12541 tcggcggaaa tacagcccgg agtggttatt gatcatggaa ttggtgtagt tataggagaa
    12601 acggcatcgg ttggcacagg aagtcttata tatcatggtg tgactttagg tgcaaaaaat
    12661 aactgtctgg gaaaaagaca tcctcaggtt ggaaataatg tggtaatagg agcgggagca
    12721 aagattttag ggtatgtttt cgtaggaaat gatgtaaaga taggtgccaa ctcagttgta
    12781 ctgagagata ttccatctat ggttacagca gccggcattc cggcagagat caaaaaaaat
    12841 aaagcggtat attatgccca aaaagaggtg gaaaaatgat tcccgacagt atcggcggta
    12901 caggcttggt tacactgtca aaaataatag atccgttcag aatatttgta aagcttgaaa
    12961 aaaataaccg tacaggaagc ataaaagacc gaccggccct ttttatgata aatgatgcag
    13021 aaaaaagggg tttcttagga aaaaataaaa agacgatagt agagccaaca agcgggaata
    13081 ccggtattgc attgagtgca atagggataa gcagaggtta tcgtgtaatt cttactatgc
    13141 cgcagaacat gagccgtgaa agaatagaac ttttaaaact ctatggggcc gatgtggtgc
    13201 ttactcctga tgaaaaaggc atgagaggag caatagaaaa agctttggaa atagtggaaa
    13261 gcaaaaatgc atacatgcct gaccaatttt ctaatcctgc caatcctttt tcgcatgaga
    13321 ttacaacagg tcctgaaatt ttaaagcagg cagattacag agtggatgct tttgttgccg
    13381 cagttggaac tggcggaacc ataacaggtg taggaagagc attaaaaaag cagcttggaa
    13441 aaaccgtgaa gatagtagca gtcgaacctt tgcaatcagc cgtattaagt ggaaacaagg
    13501 cggctgctca taaaatacag ggaataggag ccggatttat tcctaagaac tatgagcttt
    13561 ctgtggtgga tagagtcata acagtcgatc aggaagaagt atttgaaata ctaaaaaaaa
    13621 taattacata cgaaggactt tccatagggc tttcatctgc ggctaatata ctggctgcac
    13681 tcaaactctc cggtgaactt ggtggaaaat caagaataac aactgttgca tgtgatgggg
    13741 ttgaaaaata catgagcctc atatagcgaa aatcttaaaa tgtgactttt atcataaatg
    13801 ttttcatgac gtaagttact ataaaaaaag ttatttatat attaaaatga ataatggcat
    13861 tgtaatggtc ttatacttct cagggaaaag ccggacaggt tagaaaaact gttcggtttt
    13921 ttttatttag agtataattt ttttagagaa ataaaaaaat ttaataaaaa aataaaaaat
    13981 atcaatttta tacttgacaa actaaaaaat atctgttata attttgttga aaatataaaa
    14041 atatttatca gggggcgata atatgacaac agaaaaaaaa gtaagcagaa ctgtagaatc
    14101 gtattatgca ggttatgaaa tgatttatga gcttggaagc atatttatgg agcatataag
    14161 aagggtctga aaatgaagaa aatgattaag aacagctgtg attattactt ctcaaagatg
    14221 gaaaggctta cggaacatgt tttccaggag tacagaagga acggacgaat cggataggag
    14281 ggataacatg aaagagtttg aaagaatacg gaagataact tcttatgcaa gagtttttga
    14341 agaaatggaa aaagtcagca gtatctttat agaaaatatg aggagaggac tctaataatc
    14401 cggcgtaaag ccggtttttt tatttatcgt ggctttttga accaacttca ataaagtaag
    14461 ctttcctaaa tagtttcttg acggttacaa aaaagaaata ataactgtgg tagactttta
    14521 aataattgaa tagaaaaaaa ttctgaacgg aggcgcggca caaaaaagta ggaaaccatt
    14581 tatgcaagag gattgcggtt tctgaaaggt aatgccgccg aagggtacgg gacttcctca
    14641 gtccgtattg cctggttata cagataatat ctgtataact gtcacgtttt ttgtggagag
    14701 ccgtttcgga taagatgtag tgatggctct gccggatgct atttatttta ttcgtaataa
    14761 ctctccttaa gcaaggagag ttttttattt tgggtagtgc agggaagtag aaaggattat
    14821 tcccaaacat ggaggtgttc tgtgactata ggtattgtag gagcaagcgg cgaagtcgga
    14881 cgtacaatga taaaagtact tgaaaaaatg gaggtatcaa taggcgagtt aattctgctc
    14941 gcctcatcta aatcggcagg aaaagagtta atttataaag ataagcgaat aaaaattcaa
    15001 gaacttactg aagataaaat gaaagaaaaa tatgattacc ttctattttc tgcaggcggg
    15061 gatgtatcaa ggaagtatgc ccccttggct caggagtatg gaaatacagt aatagataat
    15121 tcatcggcat tcaggatgca aaaggatatt cctctggttg tacctgagat aaatgccagt
    15181 ctgcttaaag gatacacagg tatagtagcc aatccgaact gttcaacaat tcaaatggct
    15241 ttggcactta accggataca tgagaatgca gggttaaagg aagtagtagt ttctacctat
    15301 caggcggttt ctggtgcagg aaacaaggga ataagcgaac ttcaggctca acagtccggt
    15361 gacatggaga ttaagcattt tccggatcag atactcaaca atgtgatccc taagataggt
    15421 gagtatgagc ctaatggttt tacccaggaa gagatgaaga tggtgaatga aaccagaaaa
    15481 atcttcgatg ataagagcat cgatgttttt gcaacgacgg tgagagttcc tgttttttac
    15541 gggcacagtg aatcagtttt tttccgtaca aaaaaagaac tccagctttc ggaggttaag
    15601 aagctcatag aaacgtctga gaacgttgtt tacagtgatg agataattac cccggtgcat
    15661 attgcgcaaa gtgatcttac atatgtatgc agacttagaa aagcaggacc caaggaatac
    15721 atgatgtggg tagttgccga taacgtaaga gttggggcag cgactaatgc cgtaagaata
    15781 cttttgagcc atgcactgct aaatgcaggg aaatgaaaaa tgccaaaaaa aactgtacct
    15841 tttactattg aagatatcaa agctcttaca gaaaaatatc cgactccgtt ttatatttac
    15901 gatgaaaaag ccataaggga taatatacga gtatttctaa aagaattttc atggatagat
    15961 tttaaagagt attttgcggt gaaagctaca cccaatcctt cgataatgaa gattttaagt
    16021 gatgaaggct gcggtatgga ctgcagctca atgcctgagc tggtactggc tgaaagtata
    16081 ggatgttcag gcagacagat aatgtttact tccaacgata cacccaagca ggagtatgag
    16141 aaagcctttt ctttaggtgc ggttattaat ttggatgatt attcccatat aaagagtatt
    16201 gaacacatga taagtccgga cagcagcatg tgtctgagat ataatcctgg aaaccgcagg
    16261 ggaaacgaga taataggaca gctcaaagac tcaaagttcg gaatgagcag ggaacagatt
    16321 tttaaatcgg tttatgagct gaaaaagtat ggaattaaaa gtatagggct acatacgatg
    16381 atatgttcaa atgaacttga ccccaagtat tttcagatta ctgccaaaaa catgtttgag
    16441 cttgcatttg atgtaagcag tgagtttgat ataaaagttg gttttgtcaa tctgggaggc
    16501 ggaataggcg tggcatatcg tgaggaccag ccacagatg
//
LOCUS       DAOSHR010000024        16156 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_2954_length_16156_cov_5.463201, whole genome shotgun sequence.
ACCESSION   DAOSHR010000024 DAOSHR010000000
VERSION     DAOSHR010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 16156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16156
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2954_length_16156_cov_5.463201"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="PLU28_02350"
     CDS             complement(<1..353)
                     /locus_tag="PLU28_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG33940.1"
                     /translation="MMNESVWVVESAQLFNLLKFEAPFQLFPFPLMEKVFQKCFFKLR
                     DSVESDESIKQIIPYVVFINKENDVFLVKRTKKQSEKRLHEKLSVGIGGHINPSDDAI
                     QPTMTFFNGLNREIN"
     gene            complement(350..880)
                     /gene="hslV"
                     /locus_tag="PLU28_02355"
     CDS             complement(350..880)
                     /gene="hslV"
                     /locus_tag="PLU28_02355"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HOG33941.1"
                     /translation="MVLKGTTVIGVKRNGKTVICADGQVTLGNTVLKATARKVRRLGE
                     GKVVAGFAGSVADALSLFERFEIKYKNSNSNLLKAAVELTKEWRTDKALKTLEAMLIV
                     ADKESMLLISGNGEVIEPQDDAIAIGSGGPYALAAARALLENTELSAEEIAQKAILIA
                     SDICIYTNSNIVVESV"
     gene            complement(896..1777)
                     /locus_tag="PLU28_02360"
     CDS             complement(896..1777)
                     /locus_tag="PLU28_02360"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HOG33942.1"
                     /translation="MKIGILVGGPSNESEISYLSGNNILNSLNRLGYSSFLMDPRDND
                     FIEKIDSCDLVFNLIHGAIGEDGKVQGLLDLKGKKYTCSGVSVCSSTYDKYVFYTLFK
                     DVIKCPETYLTKEITAEPFDYPFVIKPRSSGSSKGVHIIHSHEQYLQAMDQELKIYDQ
                     MLIQQYIKAREITVSVLQNRSEYEILPILELRPKNEFYDYESKYTDGMTEFIVPANLN
                     NTEMESIEKACRAITSVLDLKDMFRIDGLIWNNSFYVLEVNTVPGMTNLSDLPQSAYA
                     KGMTFDQIVDTIIKNHI"
     gene            complement(1774..4029)
                     /gene="topA"
                     /locus_tag="PLU28_02365"
     CDS             complement(1774..4029)
                     /gene="topA"
                     /locus_tag="PLU28_02365"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HOG33943.1"
                     /translation="MAKKEQKYLVIVESPAKAKTIERYLGKNYEVVASKGHVRDLPKK
                     KFGVSIEKGFSPEFEIMEGKQNVIDEIKKLSKGKNIFLASDMDREGEAIAWHLSQILD
                     LPNDEPNRIIFSEITETAIKNAINSPQKIDTVKVDAQLARRVLDRVVGYKLSPLIWKI
                     IRSNNSSAGRVQSAALKIISEKEREIFQFIPKDFFKIFLLYNDYKIIITKENNKKLKM
                     ESINEAKRDEIFTYLNNKQFFVKEVKDKQLKRNPPEPFITSTLQQTAISMLGWTSKKT
                     MQVAQQLYEGVDTENGHIAFITYMRTDSTRISDIASKSAKEYILSNYGQEYCGNYKMK
                     NPKNKTQDAHEAIRPTDTQMNPSQAKALIKGDLLKLYNLVWSRFMASQSTSSSYTEKV
                     YCITDETEKYTFEISSKKLNFEGYEIFYKSGETEKEVIIPQSGNLSPVSLKSEAEQTK
                     PPSRYTEASMVKEMETLGIGRPSTYATVLSTLYDRKYIVKGEKNVLIPTISGFLVNDF
                     LNKNFPNIVDASFTANMENELDRIETGELGYCDLMENFYSSFKINLSETEHNIVKEQT
                     ELSYQSNIKCKNCEENMVLAFGRYGIYLKCLKCKETKKVSFDCLAVTLGNKVFFNADF
                     SDASAVQEEKSFGKCPKCDSDLVLKKGRYGDFIACSNYPTCKFTKNIPVRGHCPKCGS
                     EVIKLRSKKGKIFYACTNEQCKELFWYEPSDHICPECGSKLFYSYKNKSEKLFCLKDK
                     TYYDEKEMPQK"
     gene            complement(4029..5096)
                     /locus_tag="PLU28_02370"
     CDS             complement(4029..5096)
                     /locus_tag="PLU28_02370"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOG33944.1"
                     /translation="MNEGIDLASIIKDQGIKLGSLLQEKDILVKDLGADMTVKIDLKL
                     VSKRLQSTKDGKKFILLTLADKSGVLRAIDWSFPQEYDKNLRIGQIVRVSGHTVMFEN
                     RLQLNIFKEQGSLEVLDETQIYQEKFIEKSSKDPQEMILYIKNAIDTITTLGIKKLLE
                     SFFVQDNGFIKKFTELPAGMTVHHAYKNGLLEHTCTVLKISINLCSIYKGIIDEDIMI
                     AGAILHDIGKIYEYSIYPYGIEKTDNGELIGHIVMGCSMIYEKARNIEELDNKTLEHI
                     VHIVASHHGEIEYGSPVIPKTIEAQLLHFCDNMDSKVSQFKSCIDNSLKSSADSNWTE
                     YDKSLGRRLMFNKEFFAGGTI"
     gene            complement(5089..5988)
                     /gene="alr"
                     /locus_tag="PLU28_02375"
     CDS             complement(5089..5988)
                     /gene="alr"
                     /locus_tag="PLU28_02375"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOG33945.1"
                     /translation="MQILIFGYCEPYELKDYIEFSDYIVPTIFSAHFIEQAYALLGND
                     IRKFVFDIKYNTGMSRVGINDYEMDSVIDLIKKYNLRIRGFYSHYATADDLDDFVSIQ
                     YEKYLKMLEYILSKGISISLRHMSNSAAAMFFPQNSLDIVRPGIATYGLQPSSKKYIN
                     GLKPVMEIKSKIIKLNTVEKGETVGYSRTYTAKEKLQTAIIPVGYADGYFRSLSNRGR
                     VLINGEYCDILGRISMDQMTVDVTGKNVAYDDEVVLIGQSNNSFISADELADICGTIN
                     YEITSKITSRIRRKYIKGGVIFE"
     gene            complement(5946..6212)
                     /locus_tag="PLU28_02380"
     CDS             complement(5946..6212)
                     /locus_tag="PLU28_02380"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOG33946.1"
                     /translation="MNGRGTVAWVNIDNYLWNLELIRNTCKKDIMPVVKADAYGHGKI
                     ELSKAAQNEGYSKFAVAFISEAADLIKNGLTCRSLYSDTVNLTN"
     gene            6770..8269
                     /locus_tag="PLU28_02385"
     CDS             6770..8269
                     /locus_tag="PLU28_02385"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HOG33947.1"
                     /translation="MNKAEEFKKYVKKIQYYYQALSLIRWDIETGAPKKCIESRAQAI
                     GEISTYAFEMSTSEKMENFLNELSLPESMEKLSDNDRALVRVMKKDFEKQKKIPPELV
                     GQLSVASSKAQAAWEKAKINDDFSVFQPFLQEVVLLTEKMADCIGYETNRYDALLDEY
                     EPGFKSNDVKKVIGYLKKELVPFVEKITEKEVDDDYSFLKMKVDRQVQRKLSEKVLRL
                     MNYDHDAGRLDVSMHPFTTSIGANDVRITTFYRDNDFSDSFFSTVHECGHALYEQGVS
                     SELADTPLSSGASMAMHESQSRLWENIVARSHEFWEFFYPETIKIIPELSSVDMERFY
                     RGINTVKRSLIRTQADEVTYNLHIMMRFEIEEALINDRIKVSELEQIWNSKMEEYLGI
                     VPLKASEGILQDVHWSHGSFGYFPSYMLGNVYSVQIYNQMKKDIPSLKDSISNGDLKV
                     ILDWLREKIHVHGKKYEPKELLAMITGQEPDPSYFMEYIKEKYSHIYKI"
     gene            complement(8298..11297)
                     /locus_tag="PLU28_02390"
     CDS             complement(8298..11297)
                     /locus_tag="PLU28_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG33948.1"
                     /translation="MKKRLLLILFPFFFILGMSADTKHPLELIYIDNQDYRWEYLFSS
                     EELSFSDIVLKSNWQKYSSPTLPLVKPLDHNKILWVRFLLPFSEYEYSLFSNLIISDF
                     QVFIDQKEVFRSDNIAAEIKNKDTYWRNQIFFIPSAAQGQYIYYRIVSNTANIGFSGY
                     TVIGKKLDILRKIFVFDLDSLIIALILMLCGISFILFSFIRKQYRAKLLFSGTFFFLL
                     AFRILFQTNSLSYILQNSLIKNVLNDFILYLLPVSAIFLIKTFFKKKFFLFNVRFILP
                     FSIISVILSVINLIDLMNFPILIKVRSYFNFFWYIFIIFVISSIISYIFFICDKNFSR
                     TCFLYGIFFISIFVVYEILGGFGFVPWQHTYLQWGALFFSLTIIVLLLYEMTQENIRL
                     KQKLDQTVIENKNLIETDYLETQKNNGLKNIIKKYSREILDKLDTLKFENSQKYTLTL
                     LRNFTEYVSQISEEEATYDLYDFSHLKELSTIIVNIKKHNKKAFQERLVFFFLNIDFP
                     IIIKKESWALINFLNFLLLKITDFIYNSTVNITYEESPINHALILSLPLKNITRQSEL
                     MICLNQKIDFFSTKIINNLSIRASSKDTLEICLAFNPDILMPINFYDYRKSYYLRNNN
                     DFNISFLHEDSRDCDIANILEVEGYNVTIYETVEKLFYNLSFPGKDIFIFICHSIDTI
                     KKIISSNIINLQDNFTFLITSRTILSGLTISDYLFFDEIFIQPLSVEELIIKIISYGC
                     ISQHGIKKEEKLSLLDISQNNHFSAILSTMPSIYRINRQDIAQEGAFRISKEVEHVFA
                     VENNTQFKKILNLYSLFFSNQTEGDAAIRSCFKFLQEIKKLPFLKNTPFIISGSDFSS
                     NITIFNAFAELFTIHEKILGNAFNYNSNLLVSSDIFLSLNDPQKYSFRIADIFYEPST
                     KSFSILFDFYDLDEPDIKNLKNASKYFFEKAWNSYINYDFISCRKFISDAYSIYPDDA
                     LLKLYSKRCEYFISSPPKSFSQLFEIF"
     gene            complement(11375..12334)
                     /locus_tag="PLU28_02395"
     CDS             complement(11375..12334)
                     /locus_tag="PLU28_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HOG33949.1"
                     /translation="MRIDPNYSKEFEYIARAYEQAGGKVDSLLTKDMVSIIISGDKVL
                     GRNSIEGVTIDYKTEGTSLKLWMKIADNVKILKPVHMCVGFLEKYGEQNLEYFFDIGC
                     NSEVSFLSHCSFPNAQKIKHSMVSHILARSGSKVGYEDVHFHNEEGWVDLNTDYLADV
                     GTGSSYSTRFRLTKTRVGSMKVLMNVDLAQDAKAFLETKVWAKKDDKVDINEIVNLNG
                     KGSSGIAKTYVIAQDESQAKVVNEAYGNAPYSKGHIECTEIVKGNGVEVSTIPILKVK
                     NELAELTHEASVGRINALQLETLMAKGLTEEEATELIIRGFLT"
     gene            complement(12347..13066)
                     /locus_tag="PLU28_02400"
     CDS             complement(12347..13066)
                     /locus_tag="PLU28_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG33950.1"
                     /translation="MLEIKDISYCAGNRKILNKVSFTFKKDKTYAILGNNGVGKSTLA
                     RIIMGTEGYRNIHTGKIFFEGKDISELKVDQRARSGITMIWQEPARFEGISVRDYLTL
                     GGKENLSDESILKTLSSVGLNHFYLFRKVDKTLSGGERKRIELASVMIIKPKVVILDE
                     PDSGIDMMSNSMILNVIDFLRSNGSTVISITHREEIAMLSDEALLLCAGEIYAYGSPQ
                     SVSKSYKDMCDSCSHINIPLS"
     gene            complement(13082..13549)
                     /locus_tag="PLU28_02405"
     CDS             complement(13082..13549)
                     /locus_tag="PLU28_02405"
                     /inference="COORDINATES: protein motif:HMM:NF014629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HOG33951.1"
                     /translation="MIRIFLIGPLKSSFIIQGTEQYKKWLSPYTKNELIELELKKNFS
                     KLTKEEVAAYDFLRIEPFFEKDSANIVLDETGIMLSSVELSRKIVFWNNSSKKNINFF
                     VGGPWGHDSRVYAKADMVVSLSKMTFTHEMVVLFLYEQIYRAYKIINNEKYHY"
     gene            complement(13568..14449)
                     /locus_tag="PLU28_02410"
     CDS             complement(13568..14449)
                     /locus_tag="PLU28_02410"
                     /inference="COORDINATES: protein motif:HMM:NF019454.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG33952.1"
                     /translation="MQLVESVPNISEGRDEKLINSIVREVASYKNLWLVSKLSDPSYN
                     RSFFTIVGSPDSLEECLYNISRIAVEKIDMNLHRGTHPRIGAIDVIPIVPIMNIEEKQ
                     AQEVVTSLSSRISKDFALPVFLYEKSARKECFKNINNIRKGEFEGLTKKMKEQAFEPD
                     FGPKTPHKTAGATIIGLREPLLSINFSFSISDRWLIDQIRREVLIELPCSVFVSRDNY
                     SKRFSLSLNISAKEYSMIEVYDKVREIAEHFNCSITDVELASPITSSMALGALAKLTG
                     GLKSNCHTIEQFIISSL"
     gene            complement(14455..15564)
                     /locus_tag="PLU28_02415"
     CDS             complement(14455..15564)
                     /locus_tag="PLU28_02415"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOG33953.1"
                     /translation="MRNTIFRSIRTEYKERYKRFEFYIFLCYIALLTISFFAVKSANM
                     NTALESSSSKHLVLILFGIFLFFLTIILPERFLRRFIPVLFFLTVAALAFVLAAPKIS
                     GSRRWISLGFFNFQPSEYFKLAEILFLSEVLAAKNSKVYYLACLSIFFSAILIYKEPD
                     LGTTLIVLSMWLVMTIACAKHERIWKGAVWGAILASPVAFFMIQDYQRDRILSIFSPQ
                     QNATGAAYNTIQSMRAIGSGGMLGKGYMNGNMNLGNFVPEDHTDFIISVIGEEFGFIG
                     ICLILFLYAVILYRLYRGFSLSYDFFWKFYFLGSSFLIFFHVFENIGMNLGIIPVTGV
                     PLPLISWGGNQIITYSILLGLATKGCIVNKNKRQG"
     gene            15825..>16156
                     /locus_tag="PLU28_02420"
     CDS             15825..>16156
                     /locus_tag="PLU28_02420"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOG33954.1"
                     /translation="MIGDRTIFFDTLDSTNKYLLENWQVLPSETVVWAKQQQNAYGRK
                     KSFWSSNSGGLWFSVLFKPKKRPVNPWHYVRLYSLTVYDILKEYDIQAQIKWPNDVLV
                     GDKKICGIL"
BASE COUNT         5160 a         2852 c         2693 g         5451 t
ORIGIN      
        1 tcatttattt ctctgttaag cccattaaag aaagtcattg ttggttgaat ggcatcatcg
       61 gacggattta tatgtcctcc tatccccaca gaaagttttt catggagccg tttttcagac
      121 tgcttcttag ttcttttaac aagaaaaaca tcattttctt tatttataaa aaccacataa
      181 ggaataatct gtttgataga ttcgtcagat tcaacagaat cacgtagctt gaagaaacat
      241 ttttggaaca ccttttccat tagcggaaat ggaaaaagct ggaaaggtgc ttcaaactta
      301 aggagattaa ataactgagc agattcaact acccataccg attcattcat catacacttt
      361 ccaccactat gttggagtta gtgtatatac agatatcact agcaattagt atggcttttt
      421 gagctatttc ttcagccgaa agctcagtat tttcaagcag ggcccttgcg gctgcaaggg
      481 cataaggacc gcctgaccct atagctatgg catcatcctg aggttctata acctcaccgt
      541 ttcctgaaat aagaagcatg ctttccttat cagcaacaat aagcatggct tccaaagttt
      601 tcagtgcttt atcggttcgc cactcctttg ttagttcaac agctgctttt aaaagatttg
      661 aattagaatt cttatacttt atttcgaatc gttcaaaaag tgaaagagcg tcggcaaccg
      721 aaccggcaaa tcctgctacg acctttcctt ctcccagcct tctgactttt cgtgcagtgg
      781 cttttaaaac agtattgcca agagttactt gtccatctgc acaaataact gtttttccgt
      841 ttcgttttac acctataaca gttgtgcctt taagtaccat aagactacct ccggtttata
      901 tatgattttt gattattgta tcaactatct gatcaaaggt cataccttta gcatatgctg
      961 actgtggaag atcgctcagg tttgtcattc caggaacagt gttaacctca agaacataaa
     1021 aagagttatt ccatataaga ccgtctattc tgaacatatc tttgagatcc agaacagaag
     1081 taatggctcg acatgctttt tctatcgact ccatttccgt gttgttcaga ttagcaggaa
     1141 ctataaactc tgtcatgcca tctgtatact tggactcata atcataaaat tcgttcttag
     1201 gtctcagttc aagtatgggt aaaatctcgt attcagatct gttctgaagt acagacacag
     1261 ttatttcacg tgcttttatg tattgctgaa taagcatctg atcgtatatt ttgagctcct
     1321 gatccatagc ctgaaggtac tgttcatgag aatgtataat atgaacacct ttgctcgagc
     1381 cgcttgatcg tggcttaata acaaagggat aatcaaaagg ttctgctgtt atttctttag
     1441 tcagatatgt ttcgggacat tttataacat ctttaaagag agtataaaaa acatacttat
     1501 cataagtact tgaacatact gatactccag aacaggtata ttttttcccc ttaagatcta
     1561 aaagaccctg aacttttcca tcttcgccta ttgctccatg tataaggtta aaaacaagat
     1621 cacatgagtc aattttttct ataaaatcat tatctctggg atccataaga aaagaggaat
     1681 atcccagcct gttaagggag tttaaaatat tattcccact gagataggaa atttcgcttt
     1741 cattggaagg tcccccgacc aaaataccta ttttcatttt tgtggcatct ccttttcatc
     1801 atagtaggtt ttgtctttaa gacaaaaaag cttttcggat ttatttttat aactgtaaaa
     1861 aagcttgctt ccacattcag gacagatatg atcggaaggt tcgtaccaga aaagttcttt
     1921 gcactgttca ttagtacagg cataaaagat ctttcccttt tttgaccgaa gttttataac
     1981 ttctgagcca cattttggac agtgtccgcg tacaggaata ttcttagtaa acttacaggt
     2041 tggatagttg ctgcaggcaa tgaaatctcc gtatcttcct tttttcaata caaggtcaga
     2101 atcgcatttg ggacatttgc caaagctttt ttcttcctga acagccgatg catctgaaaa
     2161 atcagcgttg aaaaagactt tatttcccag cgttaccgca agacaatcaa agcttacttt
     2221 tttggtctcc ttgcacttaa gacattttaa ataaattcca taccttccaa aagcaagcac
     2281 catattttct tcgcagttct tacattttat attgctctga tagcttagtt cggtttgttc
     2341 tttgacaata ttatgttctg tttcagataa atttatctta aatgaggagt agaaattctc
     2401 cataagatcg cagtatccga gttcaccagt ttctatcctg tcaagttcat tttccatatt
     2461 tgcggtaaaa cttgcgtcta caatatttgg aaagtttttg ttaagaaaat cattcactaa
     2521 aaaaccggat atcgttggaa taagtacatt tttttcaccc ttcacaatat atttcctgtc
     2581 ataaagggtc gaaagaacag ttgcataagt tgaaggtctt ccgattccaa gagtctccat
     2641 ttcttttacc atagatgctt cagtatatct tgaaggtggt ttggtttgtt cggcttcgct
     2701 tttaagagaa actggagaaa ggttaccgct ctggggtata ataacttctt tttctgtctc
     2761 accgctcttg tagaaaattt cgtaaccttc aaaattaagt tttttagatg aaatttcaaa
     2821 ggtatacttc tcagtttcat cagttatgca gtatactttt tctgtataag agctactggt
     2881 agactggctg gccatgaaac gtgaccatac aagattataa agttttaaga gatccccttt
     2941 tataagagct tttgcctgag aaggattcat ctgtgtatcg gttggtctga tcgcctcgtg
     3001 ggcatcctga gttttatttt tcggattttt cattttataa tttccgcagt attcctgtcc
     3061 atagttactt aatatatatt cttttgcgga ttttgacgct atgtctgaaa ttcttgtcga
     3121 atccgttctc atgtaagtta taaaagcaat atgtccattt tcagtatcta caccttcata
     3181 tagctgttgg gctacttgca tggttttttt tgacgtccag ccaagcattg aaatcgcagt
     3241 ttgctgcaaa gtacttgtaa taaatggttc tggtggattt cgctttaact gtttatcttt
     3301 gacttctttt acaaagaact gcttgttatt aagatatgta aaaatttcat cccttttggc
     3361 ttcatttata ctttccattt tcaatttttt attattttct tttgtaataa ttattttgta
     3421 gtcattatac agtaaaaata ttttgaaaaa atcctttgga ataaactgaa agatctctct
     3481 ttctttttca gaaataattt ttaaagcagc agattgcaca cgtccagcac ttgagttgtt
     3541 tgaacgtata attttccata ttaacggact cagtttatat ccgacaaccc tgtctaaaac
     3601 tcttcttgcg agctgagcat caacttttac agtatcaatt ttctgtggtg agttaatagc
     3661 gttctttatt gcggtttctg tgatctcact gaaaattatt ctgttgggtt catcgttggg
     3721 taggtcaaga atctgactta aatgccatgc tatggcctca ccttccctat ccatatccga
     3781 tgcaaggaaa atatttttac cttttgaaag cttttttatt tcatcaataa cgttttgttt
     3841 tccttccatt atttcaaatt caggagaaaa tcctttctca atgctaacgc cgaatttttt
     3901 ctttggaaga tctcttacat gtcccttgga ggctactact tcatagtttt ttccaaggta
     3961 acgttctata gtttttgctt tggccgggga ttccacaatg accaagtatt tttgttcttt
     4021 ttttgccatc agatagttcc tccagcgaaa aattctttgt tgaacatcaa tcttctgccc
     4081 agagatttgt catattcagt ccagttacta tctgccgaag attttagtga gttgtctatg
     4141 caggatttaa actgagacac ttttgagtcc atgttatcgc aaaagtgtaa aagctgtgct
     4201 tctatagtct tgggaataac gggactgcca tactctatct ctccgtggtg agaagctaca
     4261 atatgaacaa tatgctcaag agtcttgttg tcaagctctt caatattacg tgctttttca
     4321 tagatcattg aacagcccat aactatatga ccaataagtt caccattatc tgttttttca
     4381 atcccatagg gatatataga gtattcataa atttttccta tatcatgaag aatagcaccg
     4441 gctatcatta tatcctcatc gattataccc ttgtatattg aacacagatt gatacttatt
     4501 tttagcacag tacaggtatg ttcaagaaga ccgtttttat aagcatgatg aacagtcatt
     4561 ccagccggta gttcagtaaa ctttttaata aagccgttgt cttgcacaaa gaatgattct
     4621 aacaactttt tgatgcctag agttgttatt gtatctatag catttttaat atatagaatc
     4681 atttcttggg gatcctttga acttttttct ataaactttt cctgatatat ctgagtttcg
     4741 tccagtacct ccaaagaacc ttgttcttta aaaatattaa gctgaagcct attttcaaac
     4801 atcactgtat gtccgcttac acggacaatc tgacctatac ggagattctt gtcatactct
     4861 tgcggaaaag accagtcgat tgctcttaat actccgcttt tatcagctaa agtgagcaaa
     4921 ataaattttt taccgtcttt cgttgactga agtcttttgc ttacgagctt aagatcaatt
     4981 tttacagtca tatccgcgcc cagatcctta acaagaatgt ctttttcctg aagaagactg
     5041 ccaagtttta ttccctggtc tttgattatg ctcgcaagat caataccttc attcaaatat
     5101 aactcctccc ttaatgtatt tgcgtctgat tctagaggta atttttgaag ttatctcata
     5161 gtttatggtg ccgcatatat ccgcaagctc atcggcagag ataaaactat tgtttgactg
     5221 tcctataaga actacctcat catcataagc aacgtttttg ccagttacat caacggtcat
     5281 ctgatccatt gatattctgc caagtatatc gcagtattcg ccgtttatca gaacccttcc
     5341 cctgttggaa agagatcgga aataaccatc agcatagcct accggtatta tagcggtttg
     5401 aagcttttcc ttagcagtgt atgttctgga gtatcctact gtttcgcctt tttctacagt
     5461 attcaatttt ataattttac ttttaatttc cataacaggt ttaagaccgt ttatatactt
     5521 ttttgatgac ggttgcagtc cgtatgttgc aattccaggc cttacgatat ctagtgagtt
     5581 ttgtgggaaa aacattgcag ccgcactatt ggacatatga cgcagtgaaa tgcttatccc
     5641 ttttgaaagt atgtattcaa gcatctttaa gtatttctca tactgtatgg aaacaaagtc
     5701 atccagatca tcagccgttg cataatgaga ataaaaacct ctaattctta gattatactt
     5761 cttgattaaa tctatgacag aatccatttc ataatcatta attcctactc tggacattcc
     5821 agtgttgtat ttgatatcaa atacaaattt tcttatatcg tttcccaata atgcgtatgc
     5881 ctgctcaata aagtgtgctg aaaaaatggt tggaacaata taatcggaaa attctatgta
     5941 atctttcagt tcgtaaggtt cacagtatcc gaatataagg atctgcaagt cagaccattt
     6001 tttataaggt cagcggcttc tgaaataaag gcaacagcaa acttactata gccctcattc
     6061 tgtgcagctt ttgaaagttc aatctttcca tgtccgtagg catctgcttt tactacaggc
     6121 ataatatctt ttttacaggt gtttcttata agttcaagat tccaaagata attatcaata
     6181 ttaacccaag ctaccgttcc cctgccgttc atttttttcc cccctgatta aatctagcag
     6241 tatatatata ttctcaatat tttttgagac ctttaatatc tttatctttg attttattat
     6301 aaaatgcctc ttctgctcga ctattccttc tatttcggat atgggtatag tatcgtattt
     6361 ggcagcataa gtcatatagc ctgcaaataa aatgtctgct gatgtgttaa cagaaacttt
     6421 atagcgcaga accgttaaag gtagatagaa aaaaatactt gcgaacataa ctgcacagat
     6481 ccaaccagtg acaacacctg gtaacagaga gtaaaaccat gaaagagtaa aaaatccaat
     6541 gaaaataaaa accgcacaaa aaactcgtac ttttttatta tacctaaaat tatatatcat
     6601 aatataatac tcctttgcac caaaagtttc aattataatt atacattcgt agacagtcaa
     6661 tatactttaa atttatgtgt tgatactttt aaaattaata ttagggtgat tgattatttg
     6721 ctgaaaattt gctagtattt aataaacaga agctaaggag gtgtaacaag tgaataaagc
     6781 agaagagttt aaaaaatatg tcaagaaaat tcagtactat tatcaggcat tatctcttat
     6841 tcgctgggat atagaaacag gtgcacctaa aaaatgcatt gaaagcagag cacaggccat
     6901 aggtgaaata tcaacgtatg cgttcgaaat gagtacatct gaaaagatgg agaacttttt
     6961 aaatgagcta tctttacctg aatcaatgga aaagttaagc gataatgaca gagctctggt
     7021 aagggtaatg aagaaagatt ttgaaaagca aaagaaaatt cctccggaac ttgtcggaca
     7081 gctttcagtg gcctcttcta aagctcaggc agcatgggaa aaagcgaaaa tcaatgatga
     7141 tttcagcgtt tttcagcctt tcctgcaaga ggttgttttg ctgactgaga agatggcaga
     7201 ctgtattgga tatgaaacaa atagatacga tgctctactg gatgaatatg aacctggttt
     7261 taagagcaat gatgtaaaaa aagttatcgg ctatctcaaa aaggagcttg taccgtttgt
     7321 tgaaaaaata acagaaaaag aggttgatga tgattattct ttcttgaaga tgaaagttga
     7381 taggcaggtt cagagaaagt taagcgaaaa ggttttaaga ttgatgaact atgaccatga
     7441 tgccggacgt cttgatgtat ccatgcatcc ttttacaact tctattggag ctaacgatgt
     7501 tagaataaca actttttaca gggataatga tttttcggat tcattttttt ctacagttca
     7561 tgaatgtgga catgctcttt atgagcaggg ggtatcatca gagcttgcag atactcctct
     7621 ttcaagtgga gcttcaatgg ccatgcatga atctcagtcg cgtttatggg aaaatatagt
     7681 ggcacgcagt catgaatttt gggagttctt ttaccctgag acgattaaga tcattccaga
     7741 gctttcaagt gttgacatgg aaagatttta tagaggcata aatactgtta agcgatccct
     7801 aataaggaca caggctgatg aagtcacata taatcttcat ataatgatga gatttgagat
     7861 agaagaagct cttataaacg acagaataaa agtttcagag cttgaacaga tttggaactc
     7921 caaaatggag gagtatcttg gaatcgttcc tttaaaggct tccgaaggaa ttttgcaaga
     7981 tgtacattgg tctcatggca gtttcgggta ttttccttcc tatatgcttg gaaatgtcta
     8041 ctccgtccag atatacaatc agatgaaaaa ggatattcca tctctaaaag acagtatttc
     8101 caatggagat ttgaaagtca tattagactg gcttagagaa aaaattcatg ttcatggaaa
     8161 aaaatatgag cctaaagagc ttcttgctat gataacaggt caggaaccag atccatctta
     8221 ttttatggaa tatataaaag agaaatactc ccatatttac aaaatataat acagggctaa
     8281 gccctgtatt atattagtta aaagatttca aaaagctgtg aaaaactctt tggtggagaa
     8341 gaaataaaat actcacatcg ctttgaatat agttttagta aagcatcatc gggatatata
     8401 ctgtaagcat cacttatgaa tttcctgcaa gaaataaagt cataatttat ataggagttc
     8461 caggcttttt caaaaaagta ctttgaggca tttttaaggt tttttatatc gggttcgtca
     8521 agatcataga agtcaaaaag aatagaaaaa cttttagtag aaggttcgta aaaaatgtcg
     8581 gctattctaa aactatactt ctgtggatca tttaaagata aaaagatatc tgatgaaacc
     8641 aaaagattag aattgtaatt aaaggcatta ccgagaatct tttcgtgtat tgtaaataat
     8701 tcagcaaaag cgttaaaaat agtaatattg ctgctaaaat cacttccgga aattataaaa
     8761 ggagtattct ttaagaaagg cagtttcttt atttcctgaa gaaatttgaa gcaacttctt
     8821 attgcagcat ctccttcggt ctgatttgaa aaaaataaag aataaagatt cagtatcttt
     8881 ttgaattgag tgttattttc tactgcgaat acatgttcta cttcttttga aatacgaaat
     8941 gctccttctt gtgcaatatc ttgtctgttt attctgtata tacttggcat agtgcttagt
     9001 atggcactga aatgattatt ctggctaata tccaataaag ataacttttc ttcttttttt
     9061 atgccatgtt gagatatgca accgtaagat attattttaa taattagttc ttcaacagat
     9121 agaggctgga taaatatctc atcaaaaaaa agatagtcgc ttattgttaa tcctgaaaga
     9181 atggtccgtg aggtgatcaa aaaagtaaag ttatcttgta agttaataat gtttgatgaa
     9241 ataatttttt taattgtatc tatcgaatga catataaaaa taaaaatatc ttttccggga
     9301 aaacttaggt tataaaatag tttttcaaca gtttcgtata ttgtgacatt atatccctca
     9361 acttcaagga tatttgctat atcacaatca cgactatcct cgtgtagaaa agatatatta
     9421 aaatcattgt tattcctaag gtaatatgat tttctataat cataaaaatt aatgggcatt
     9481 agaatatcag gattgaaagc caaacaaatt tccaaagtat cttttgaaga agctcttata
     9541 gaaaggttat ttattatttt tgttgaaaaa aaatcgattt tttgatttaa acatatcatt
     9601 aattcgcttt gcctagtaat attttttaaa ggcagtgaaa gtataagtgc atgattaatc
     9661 gggctttcct cataggttat attaacagta gagttatata taaagtcagt tattttaagc
     9721 agaaggaagt ttaaaaaatt tatcaaagcc caactttctt ttttaataat tatcggaaaa
     9781 tcaatattta aaaaaaagaa aactaatctt tcctgaaaag cctttttatt atgctttttt
     9841 atatttacta ttatcgtaga tagctctttc agatggctaa aatcatataa atcataagtc
     9901 gcctcttctt ctgatatctg agatacatac tcagtaaaat ttcttaatag agttaaagta
     9961 tacttttggg aattttcaaa cttaagcgta tccagtttat ctaatatttc tcttgaatac
    10021 ttttttatta tgttttttaa gccattgttt ttttgagtct caaggtaatc tgtttctata
    10081 agattcttgt tttcaataac agtttggtca agtttttgtt tcaatctaat attttcttgg
    10141 gtcatttcat ataagagtag aactattatt gtcagagaaa agaataatgc accccactga
    10201 aggtaggtgt gctgccaagg gacaaatccg aatcccccca gtatttcata cacaacaaat
    10261 attgaaataa agaaaatccc gtaaagaaag caagttcgtg aaaaattctt gtcacatata
    10321 aaaaaaatgt atgaaattat agacgatatg acaaatataa taaaaatata ccagaaaaaa
    10381 ttaaagtatg atctaacctt tattagtata ggaaaattca tcagatcaat aagatttata
    10441 acagacaaaa ttacggaaat aatagaaaaa ggtaatatga accgtacatt gaagagaaaa
    10501 aacttttttt tgaaaaaagt ctttatcaga aagatagccg atacagggag tagataaaga
    10561 ataaaatcat taagaacatt ttttattagg ctattttgta gtatatatga aagagagttg
    10621 gtctgaaaaa gaattctgaa agctaaaaga aaaaagaaag ttcctgaaaa aaggagtttg
    10681 gcacgatact gtttccttat gaaagaaaac agaataaaag agataccgca gagcattagt
    10741 ataagagcta ttataaggct atccagatca aaaacaaaga tctttctaag tatatcaagt
    10801 ttctttccga taacggtata tccagaaaaa ccaatattag cagtattaga tactattcta
    10861 taataaatgt actgtccctg tgcagctgag ggtataaaga aaatttggtt tctccaataa
    10921 gtgtctttgt ttttaatttc tgcggcaata ttatctgacc tgaaaacttc tttttggtcg
    10981 ataaatacct gaaagtcaga tattataaga ttggaaaata agctgtactc atattcggaa
    11041 aaaggaagga gaaagcgaac ccaaagaatt ttattatgat ctagaggttt taccaaaggt
    11101 aatgtgggag aagaatactt ttgccagttg cttttaagga caatatccga aaatgaaagt
    11161 tcttctgatg agaacaagta ctcccaccgg taatcttgat tgtctatata aataagttcc
    11221 aaaggatgtt ttgtatccgc agacatcccc aggataaaaa agaaaggaaa aagtataaga
    11281 agtaatcttt ttttcattat aaaccccaat atttttccat atataaagaa attatactat
    11341 ataagaataa aaaaatcttc ctgttaggaa gattttatgt tagaaatcca cgtattatca
    11401 gttcagtagc ttcttcttct gtaagtcctt ttgccataag tgtttcaagt tgcaaagcgt
    11461 ttattcttcc tacagaagct tcgtgagtaa gttctgccaa ttcattttta actttaagta
    11521 taggtattgt agaaacttct acgccatttc cttttactat ttcagtacat tcaatgtgtc
    11581 ctttggagta aggagcgttt ccataggcct catttacaac ctttgcctga ctttcatcct
    11641 gtgcaatcac ataagtttta gctatcccgc tagagccttt accgttaaga ttgacaattt
    11701 catttatatc tactttgtcg tctttctttg cccacacctt ggtttcaaga aaagccttag
    11761 catcttgtgc tagatcaaca ttcataagaa ctttcataga gcctacacga gttttagtga
    11821 gtctgaacct tgtagagtat gaagaacccg tacctacgtc agccaaataa tcggtgttaa
    11881 gatctaccca accttcttcg ttatgaaagt gaacatcctc atatcctact ttcgaaccgc
    11941 tgcgtgccaa aatgtgcgaa accatagaat gttttatctt ctgggcgttt ggaaatgaac
    12001 aatgggataa aaaagagact tcagaattac agccgatatc aaaaaaatat tcaagatttt
    12061 gttccccata cttttcaaga aaacctacac acatatgaac gggttttaat attttaacat
    12121 tatctgcaat tttcatccat agttttaaag aagtcccttc ggttttataa tctattgtaa
    12181 cgccttcaat actgtttctt cctagtacct tatctccgct tattattatt gaaaccatgt
    12241 ccttggtaag aagggaatca acttttccgc ctgcctgttc ataagctctg gcaatatatt
    12301 caaactcttt tgagtagttc ggatcaattc tcatataggc ctcctattac gataaaggaa
    12361 tattgatatg tgaacagctg tcacacatat ctttatacga tttgcttacc gactgaggag
    12421 aaccgtaggc ataaatctca cctgcacaaa ggagtaaagc ctcatcagaa agcatggcaa
    12481 tctcttctcg gtgagttatg gatattaccg ttgaaccatt actcctgaga aagtcgataa
    12541 cgttaagaat catggagtta gacatcatat ctatacctga gtctggttca tccaaaataa
    12601 ccactttagg ttttattatc atcaccgatg caagttcaat tctttttctc tcaccgccgc
    12661 taagtgtctt atcaactttt ctaaataaat agaaatgatt cagacctact gaagaaaggg
    12721 tttttaaaat agactcatcg gataagtttt ctttccctcc aagtgtaaga taatcacgaa
    12781 cagaaattcc ttcaaatctg gctggttcct gccatatcat ggttataccg gatcttgctc
    12841 tttgatcaac tttcagttct gaaatatctt ttccttcaaa aaaaatcttt ccggtatgaa
    12901 tgtttctgta tccttccgtt cccattatta tccgggccag agtagatttt cctacaccgt
    12961 tgtttcctaa aatagcataa gttttatctt ttttaaaggt gaaacttact ttattcagga
    13021 tttttctatt tcctgcacaa tatgagatat cttttatctc tagcataaac acctcttact
    13081 tttaataatg atatttttca ttattgatga ttttataggc tctataaatc tgttcgtaaa
    13141 gaaaaagtac aaccatctcg tgggtaaagg tcatttttga taaggataca accatatccg
    13201 cttttgcata aactctactg tcatggcccc agggacctcc aacaaaaaag ttaatatttt
    13261 ttttagaaga gttgttccaa aaaactatct tccggctcaa ttcaacagaa gaaagcatta
    13321 ttccggtctc atcaagcact atattagctg aatccttttc aaagaaaggt tcgatcctta
    13381 aaaagtcata agcagccact tcttcctttg taagtttgct gaaatttttt tttagctcca
    13441 gctcgatcaa ttcatttttt gtataaggac taagccattt tttgtactgc tctgtaccct
    13501 gaattataaa actactcttt aatggtccta taagaaaaat tcgtatcatg tgaagatgat
    13561 ttaaaactta aaggctgcta ataataaact gttctattgt atggcagttt gatttaagac
    13621 cgcctgtcaa tttagctaaa gctcctaaag ccatcgaact ggtgatagga gaagcgagtt
    13681 ctacatctgt aatggaacag ttgaaatgtt cggcaatctc tcttacttta tcgtaaacct
    13741 ctatcatact gtactctttg gcactgatgt taagagaaag actaaagcgc tttgagtagt
    13801 tatctctgct tacaaaaaca ctacaaggca gttcaataag aacttctctt cttatctggt
    13861 caatgagcca tctgtctgat atgctgaaag aaaaattgat gctgagcagt ggttctctta
    13921 agcctattat agtagctccg gctgtcttat gaggggtctt tggaccaaaa tcaggttcaa
    13981 atgcctgttc cttcattttt ttagtcaatc cttcaaactc accttttcgt atattgttta
    14041 tatttttaaa acattccttt cgtgcagatt tttcataaag aaaaaccgga agtgcgaaat
    14101 cctttgatat tcttgaagaa agggaggtaa caacttcttg agcctgcttt tcttcaatat
    14161 tcataatagg tactataggg ataacatcta ttgctcctat tcttgggtgt gtacctcggt
    14221 gcaagttcat atctattttt tccacagcta tacgtgaaat attgtacaga cattcttcaa
    14281 ggctgtctgg actacctact attgtgaaaa aacttctgtt gtacgatgga tccgaaagct
    14341 tggaaactag ccagaggttt ttataagaag ctacttctct gacaattgag tttattaatt
    14401 tttcatctct tccttcactt atattaggta ctgattcaac taactgcatt tttttcaccc
    14461 ctgtctttta ttcttattta ctatgcatcc tttagtagcc aacccaagca atattgagta
    14521 cgttattatc tgatttcctc cccaagatat caatggaaga ggtacacctg tcacaggtat
    14581 tattccaaga ttcatcccta tattttcaaa gacatgaaaa aatataagaa aactgcttcc
    14641 aagaaaataa aacttccaga aaaaatcata gctaaggcta aatccgcggt atagtctgta
    14701 caaaataact gcatacagaa aaagtatcaa gcatatgcct ataaagccaa actcttctcc
    14761 tatgacagag attataaaat cggtatgatc ctctggaaca aaattaccca gattcatatt
    14821 tccgttcatg taacctttgc ccagcatccc accggatcca atagctctca tggactggat
    14881 tgtattatag gctgcacctg tagcgttttg ctgtggagaa aatatgctga gtattctatc
    14941 tctctgataa tcctgtatca taaaaaaagc taccggagaa gcaaggattg ccccccagac
    15001 agcacctttc caaattcttt catgcttggc acaagcaata gtcattacaa gccacattga
    15061 aagaacaatc agagttgttc caagatcagg ttccttatat ataaggatcg ccgaaaaaaa
    15121 tattgacaag caagccaaat aatatacttt agaatttttc gctgcaagca cttccgaaag
    15181 aaaaagaatc tctgccagct taaagtattc tgaaggctga aaattaaaaa aaccgagact
    15241 tatccaacgc ctggaaccgg aaatcttggg agctgccaaa acaaatgcca gtgcggcaac
    15301 cgtcagaaaa aaaagtactg gtataaatct ccttaaaaac ctttcaggga gaataattgt
    15361 gaggaaaaaa agaaaaattc caaacagtat aagaacaagg tgtttagagg atgaagactc
    15421 aagagctgta ttcatatttg cacttttgac tgcaaagaaa cttatggtga gtaaagctat
    15481 atagcaaaga aatatataaa attcaaatct tttatatcgt tccttatact ctgttcgtat
    15541 agatctaaaa atagtattac gcatctcttc ctcacttatc gttcttttta aagtattata
    15601 tcatgatcaa gtatatttac ggttaataaa ttaaaaataa attggtatga agcattatca
    15661 actatcggta tatctttaaa aagcttaaaa agagaagttt gaccggccgg caaaaattta
    15721 tatataatta acagatcagg gaatatttta tttgcaaaat cttattaaga catgtataat
    15781 aaaagtatct aaaagtaatt taatgatatt taggattgat aaatgtgatt ggtgacagaa
    15841 caattttttt tgatactctg gattcaacaa ataaatatct gttggaaaac tggcaagttc
    15901 ttccttcaga aacagttgtc tgggcaaaac agcagcagaa tgcttacggg cggaaaaaaa
    15961 gtttttggag ttctaattct ggaggattat ggttttctgt gctttttaaa cctaaaaagc
    16021 gtcctgtgaa tccgtggcat tatgtaagac tatatagtct taccgtttat gatattctaa
    16081 aagaatacga tattcaagct cagattaagt ggccaaatga tgtactggtt ggagacaaaa
    16141 aaatatgtgg tatact
//
LOCUS       DAOSHR010000025        16096 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_2971_length_16096_cov_5.597656, whole genome shotgun sequence.
ACCESSION   DAOSHR010000025 DAOSHR010000000
VERSION     DAOSHR010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 16096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16096
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2971_length_16096_cov_5.597656"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1001)
                     /locus_tag="PLU28_02425"
     CDS             complement(<1..1001)
                     /locus_tag="PLU28_02425"
                     /inference="COORDINATES: protein motif:HMM:NF016488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG33955.1"
                     /translation="MDKVNDLFYGSDDGYYIQEYGRRLELYEKFTKKVQDLIYELLKQ
                     TEIEIENISSRVKTLESFEGKLQRKNYKDPFREMTDLSGVRIILYYKEDIDKLGEIIK
                     REFDVDWDNSVDKSKLIDSDKIGYLSTHYIVSVAKDRLKLTEWRLFEGLKCEIQIRTI
                     LQHAWAAIDHKIRYKPSNKVPKKLERKLFLLAGLLELADDEFSFIKTLTTEMEGYYSM
                     MIKANNLKNVDINLTSLEVFLELTEKKYNFMDIIEKAGFVNYENSSELSSAEKEKIKS
                     ASKTLLLEVLNALEFDSIEDLQKILDDSISWFEEKFTALKALFKNFDVYITVYDVVMA
                     "
     gene            1153..>1361
                     /locus_tag="PLU28_02430"
     CDS             1153..>1361
                     /locus_tag="PLU28_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG33956.1"
                     /translation="MDNNTFYKQDKILHRILYTANMSKRSSTPADISSDLLKILFEEM
                     KATRVIFYQILQKKQSIKKLVSFET"
     assembly_gap    1362..1461
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1462..2355
                     /locus_tag="PLU28_02435"
     CDS             <1462..2355
                     /locus_tag="PLU28_02435"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOG33957.1"
                     /translation="LTSMEKKVKESERKYQKLFSNMTQGVIILKLIHDSEKNLKDFSI
                     EDINSVAKKTFKNTALLKNKECDFFTPEEKKLFMEILKKAEKSKKTFKTEITLQSQRV
                     IDLVAYMISKNQIAFVTQDITAKKKLEEELKTYASIDELTGIFNRRVGISLLEKEIMQ
                     SQRRNSPLSIIYIDINGLKNTNDRFGHSMGDELITNATKIIKKCARKSDIIFRMGGDE
                     FLIILPDCKAEKAEVVWKRIRNECDQFNKNSDTPYKISMSHGTATYSGEQTIDEFVNQ
                     ADSQMYKEKILYKKETEKSSV"
     gene            complement(2382..3446)
                     /gene="gap"
                     /locus_tag="PLU28_02440"
     CDS             complement(2382..3446)
                     /gene="gap"
                     /locus_tag="PLU28_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOG33958.1"
                     /translation="MAVKVAINGFGRIGRLVFNALYDQGLLGSKFDVVAVVDVSTDAS
                     YFAYQLKYDSIHGKFKGALSTQKSDSSLEADDILVVDGNKIKCLAAPKEGPAKLPWAE
                     MGVEYVIESTGLFTESEKAKAHLSAGAKKVIITAPGKGDVKTIVMGVNENEYNPSLHH
                     IVSNASCTTNCLAPLVHVIMKEGIGIETGLMTTIHSYTATQKTVDGPSKKDWRGGRAA
                     AINIIPSTTGAAKAVGEVIPAVKGKLTGMSFRVPTPDVSVVDLTFRAARDSSIEEIDS
                     LLKKASKTYLENYLGVADEEVVSTDFIHDNRSSIYDSLATLQNNLKGEKRFFKVVSWY
                     DNEWGYSNRVVDLLKYMAQK"
     gene            3653..3985
                     /locus_tag="PLU28_02445"
     CDS             3653..3985
                     /locus_tag="PLU28_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG33959.1"
                     /translation="MSQIFGENTGKGFIKEEPNDIFVKALESAIFKAPVRMEEKIFLS
                     DLWIITSLPEDLMVNIIENHAEKLNIPQDINFILDDKKNKKIWERPVSERVDKHQSKP
                     ASDEEAKK"
     gene            4175..4447
                     /locus_tag="PLU28_02450"
     CDS             4175..4447
                     /locus_tag="PLU28_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOG33960.1"
                     /translation="MMQVKPLGKRVLIKPITEEKRTEGGIVLPDSAKEKPQKALVIAV
                     GNLDAEYDLKAGDKVIFSKFAGNEIKIEDVDHILIDIEDILGKVVD"
     gene            4473..6092
                     /gene="groL"
                     /locus_tag="PLU28_02455"
     CDS             4473..6092
                     /gene="groL"
                     /locus_tag="PLU28_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585059.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOG33961.1"
                     /translation="MAKMLKFDEEARRSLEAGVDAVANAVKITLGPKGRNVVLEKSWG
                     SPTITNDGVSIAKEIELKDKFENLGAQLVKEVASKTNDVAGDGTTTATVLAQGMIKEG
                     LKNVAAGANPMLMKIGIQKAADRVVEEIKKMSRKLTGSEDIANVAAISANNEEIGNLI
                     AEAMDKVGQDGVITVEDSKTMETYVEFTEGMQFDRGYISPYFVTNADKMECEVKEPFI
                     LITDKKISSVKPIIPILEKVAQAGKPLVIIAEDIEGEALSTLVLNKLRGTLDSVGIKA
                     PGFGDRRKAMLEDIAILTGGTVITEDLGLTLENAIIDDLGKADVIRIKKDDTIIVTGK
                     GDPKKIQERVKQIKAQIDVTTSEYEKETLQERLAKLAGGVAVIKVGAATETELKEKKH
                     RIEDALSATRAAVEEGMVAGGGVTLLRAAKGVESLIAQLEGDIKVGARVVLKSLDAPV
                     RQIAINAGVEGAIIIDRVLAKDDPSYGYDALKDKYVNMFDAGIIDPTKVTRSAVQNAA
                     SIAAMLLTTEVLVVEEPKPDAPAPAPQMPEY"
     gene            6401..7435
                     /locus_tag="PLU28_02460"
     CDS             6401..7435
                     /locus_tag="PLU28_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG33962.1"
                     /translation="MLSGITQGGSEYQAQLDTDGIIYVQILDTFSERGKNDSKEWIDF
                     HAIVNYKFVYVKTGEVLNSRKIDAYSRAYIEKDISVQQAFRTSKLSAVDFISQEISKD
                     MSHAFILSAKVSGEKWLDLFYIDSGTNYGISENMTFNLVENLSNTAGTYEVIEGKAVV
                     VEAFPDSSIIKVLEKPLRFPISASTTHLIENPSIKLQRKSFRLSACLDGLKIPGIFIR
                     SGYDGYTGLFYSSFMQTGLQDYSVSMNLGVDGGYLIDINGLLKSGVSIGAACGYDFQN
                     NSGKNFNPFTPESSDFYLFIPFCASLYLKINDSLSFVSDVTYRFNIYGDKKIFSHEPY
                     VLRAGLEIFY"
     gene            complement(7429..7989)
                     /locus_tag="PLU28_02465"
     CDS             complement(7429..7989)
                     /locus_tag="PLU28_02465"
                     /inference="COORDINATES: protein motif:HMM:NF024248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOG33963.1"
                     /translation="MEDVKILDEIATIKAIAHPLRIRIIEEFSGKAYTAKQIADKMKE
                     SPAKIYYHIIEMESVGILRLVRTVENSGILEKYYEPVARYFKISKELLSNDKEFKSET
                     LKIFDNLFKNMDRDYYSYLSKIKETHEKFPKDSGISYITMHLDETKFKEFTFKLTELF
                     HEYSKFAESDDTKEYKAGFIVFPKNQ"
     gene            complement(8067..8189)
                     /locus_tag="PLU28_02470"
     CDS             complement(8067..8189)
                     /locus_tag="PLU28_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG33964.1"
                     /translation="MKRELIGRKDENYEILRKEVKSSFEFILNDPQSIKLFMGF"
     gene            complement(8202..8324)
                     /locus_tag="PLU28_02475"
     CDS             complement(8202..8324)
                     /locus_tag="PLU28_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG33965.1"
                     /translation="MKKEDFCFRNDDIGDLKAFHNDQYSLMNMSSSLMKIFLGV"
     gene            complement(8594..9265)
                     /locus_tag="PLU28_02480"
     CDS             complement(8594..9265)
                     /locus_tag="PLU28_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG33966.1"
                     /translation="MQKVGMLNESTLHREIIRYCYTPGDILEFPVKGYFADIYRKGVL
                     IEVQLGQFSSMKDKLKDLLCEYRIKVVYPIVLCKYIEKRSTDGKVISKRKSPKNGCRY
                     DLFDQTIRIAQFIGNPNLSFELIYINLLEVRIDDGKGSYRRKGVSISDRFLLSVQGSE
                     KIEKKEDFLDFLPEGASKFTNKELTQALGIKISTARKMTYSLNACGIISQTAKIGNEK
                     VWSIL"
     gene            9385..10197
                     /locus_tag="PLU28_02485"
     CDS             9385..10197
                     /locus_tag="PLU28_02485"
                     /inference="COORDINATES: protein motif:HMM:NF017880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF952 domain-containing protein"
                     /protein_id="HOG33967.1"
                     /translation="MIIFHIIDKTELENIEGSRYYKPASLENEGFIHCSRINQLVRVA
                     QSHYKQRNGIFIMAIESTEAGSPVVFEDLYNLNEKYPHIYGPVEMTAVKNIYPMEETA
                     DGFLLPEKAVIECSKKIFTSEGFQSLITRAVPGDTLHISNFISDLLIERPLEKDLHFS
                     EEDILNINNSAESILLLSRKDGRLDGLLYVKRKNKKDVYIRALYRTDFSGESMIAYLK
                     DFETQNGCYDDKDLTFYTSNSFIKSLCVKHGLKTAKPDTPFSADIPHKEDEK"
     gene            10197..11180
                     /locus_tag="PLU28_02490"
     CDS             10197..11180
                     /locus_tag="PLU28_02490"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HOG33968.1"
                     /translation="MFRVDAHFDLLCFVYEKRAKGETRVIEKYFYEDWLKNNFNIIVS
                     SIFIDSIFIPEMALRNALDQISCLYEEIAETPEKIMLCKNYIDIEKAEKEKKIGILMS
                     LEGSEPIGNDLFLLRTFYELGVRFIGLAWNRRTYCTDGIPVCKDVRKQYGLTDLGIDV
                     LAKAKELGMIVDVSHLTDQGFEDVMTFYDMPVIASHSNCRSLTGHLRNLTDEQMKKIA
                     QRGGVVGINSFSLFLNMEPLKASISDMAEHMNHMRSIMGTDHIGLGFDFCSHIRSSLY
                     ESEEKDKFDAMNGYNEFFILDKELSKKGFSQDEREKVYSKNFLRLYNQILQ"
     gene            11475..12614
                     /locus_tag="PLU28_02495"
     CDS             11475..12614
                     /locus_tag="PLU28_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HOG33969.1"
                     /translation="MDFNLTKEQLMVKKMIRAFTENEVKPLAAEVDETERFPQETVDK
                     MKRYGLLGMCMPKNYGGAGADELSYAICVEELSRYCATTSVIFSAHNSLSCWPIMKFG
                     TEEQKQKYLVPLAKGEKLGAFALTEPNAGTDASSQATSAVKDGDYYIINGSKIFITNG
                     GKADIFVIFAMTDKSLGTKGITSFIVERNTPGFSIGKIEKKMGIRGSSTAELVFQNMR
                     VHKDNMLGKEGKGFSIAMQTLDGGRIGIASQALGIAQGALDETIKYIMQREQFGKSLS
                     KFQAIQFDIADMVTRVDAARFLVYRAACAKQEGKPYSVLAAQAKLFAAETAMYVTTKA
                     VQLHGGYGYTKDYPVERMMRDAKITEIYEGTSEVQRMVIAGNTLK"
     gene            12636..13427
                     /locus_tag="PLU28_02500"
     CDS             12636..13427
                     /locus_tag="PLU28_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOG33970.1"
                     /translation="MKIIVCIKQVPDTTEVRIDPVKGTLIRDGVPSILNPDDNSALEE
                     ALRIKETLGQDTTVTVITMGPPQAEEILRQAMAMGADSAYLLSDRAFAGSDTWATSNA
                     IAAAIKSIDNFDIVFCGRQAIDGDTAQVGPQIAQRLGMAQVTYVQSLRVEENSIIVDR
                     ALEDGYETIQVTTPVLLTAIKELNTPRYMSINGIFDAFREKEIKVLGIKDIGLDPALT
                     GLTGSPTQVRKSFTPEPKGKGEMLTGSVKEMSDQLVIKLKQKHLI"
     gene            13441..14475
                     /locus_tag="PLU28_02505"
     CDS             13441..14475
                     /locus_tag="PLU28_02505"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOG33971.1"
                     /translation="MERTPEYLAQYKNVWVFAEQRNGKLMPVVIELMGEGKKIASQIG
                     TELCAVLLGSDVKKLADELISYGADKVYLCESELFKNYTTDGYYKTISELINEYKPEV
                     FLIGATHIGRDLAPRISATVDTGLTADCTKLEVDPEDKKLLQTRPAFGGNIMATIVCP
                     KHRPQMSTVRPGVMKKAEKDSSRTGKIITVSPKLSSQDIRTKVLNIVKMVKTNVDIQQ
                     AEIIVAGGRGLGKKEGFELIEKLAEKVGGVVGASRAVVDSQWITQDHQVGQTGKTVRP
                     KVYIACGISGAIQHLAGMQDSDCIIAINKDPQANIFKIADYGIVGDLYEVIPQIIKAL
                     DNIDELSKSV"
     gene            14486..14896
                     /locus_tag="PLU28_02510"
     CDS             14486..14896
                     /locus_tag="PLU28_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HOG33972.1"
                     /translation="MKYAEIPSGYVFEAKKIITAHMVDTFAQITGDKNPIHLDEEFAK
                     TTVFGRRIAHGILTIGLVSEVLGMEFPGKGTVYMKQDAKFLKPVYLDEEITVRVAVKE
                     KIDEKFRLVLTTQVIKSDGQLAVDGEALVQFKVL"
     gene            15066..15713
                     /locus_tag="PLU28_02515"
     CDS             15066..15713
                     /locus_tag="PLU28_02515"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HOG33973.1"
                     /translation="MEKYAAFFDLDKTLLVTNSGNLFFRYCYRLDQIKFWELIKLIPY
                     PLLYKLDMLNVEALARKLVLRFKGASKEEIYEFTGMWVKNMVKDYFRKSAVQELNLHK
                     EKGAHTVMISASTHNLCYPIQKYLGIEELICTDIKFENNIFTGDLGVHCYAQEKLTRA
                     ENFCKNHGYSLEDAYFYSDSYSDLHLLEEVGHPVCVDPDMILKKTAKEKKWPILY"
     gene            complement(15740..>16096)
                     /locus_tag="PLU28_02520"
     CDS             complement(15740..>16096)
                     /locus_tag="PLU28_02520"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOG33974.1"
                     /translation="VNDTYGHQKGDEVIELIGKISRSYDKEGVVAGRYGGDEFLYILS
                     GKDEKDVVQFCKKIKQTINSSRIFRYEDKKFISVSIGYYCNVASDDWRECIKKADEAL
                     YAAKKSGKNSIKKYEK"
BASE COUNT         5286 a         2985 c         2990 g         4735 t
ORIGIN      
        1 gccatcacta catcatacac cgttatatac acatcaaagt ttttaaaaag tgccttgagt
       61 gcggtaaact tttcctcaaa ccagcttatg gaatcgtcaa gaatcttttg aaggtcttca
      121 atactgtcaa attctaatgc attaagaact tcaagaagca gagttttgct tgcacttttg
      181 attttctctt tctctgcaga gcttagttca ctgctatttt catagttcac aaaacccgct
      241 ttttcgatta tatccataaa attatacttt ttttctgtaa gctcaagaaa aacttcaaga
      301 gaagtaaggt ttatatccac atttttaagg ttgttggcct ttatcatcat ggagtagtat
      361 ccttccatct ctgttgttag cgtcttgata aatgagaact catcgtccgc aagctcaagc
      421 agtcccgcaa gaagaaaaag ctttctttca agctttttag gaaccttatt tgaaggcttg
      481 tatcttatct tatgatctat tgcagcccag gcgtgctgaa gaattgtcct tatctgtatt
      541 tcacatttca gaccttcaaa cagcctccat tcggtaagct taagcctgtc ctttgctaca
      601 cttactatat aatgggttga aagataccct atcttatccg aatcgatcag ctttgattta
      661 tcaacagagt tatcccaatc aacatcaaac tctcttttta ttatttcccc gagcttgtcg
      721 atatcctcct tataataaag aatgatacgt actccgctaa ggtctgtcat ttctctgaaa
      781 ggatctttat aatttttcct ttgcagctta ccttcaaagc tttctaaagt ttttacacgt
      841 gaggatatgt tttcaatttc aatttctgtc tgttttaaaa gttcatagat aaggtcctgc
      901 acctttttgg taaatttttc atacaactcc aaacgcctac cgtactcctg aatatagtag
      961 ccgtcgtcag agccgtaaaa aagatcgttc actttatcca tttggtttac ctccatatct
     1021 taacactact cctattatat atcataacaa cttaaaattt cacaataaaa taactttttc
     1081 catttaataa tattacgtaa attatgttat aattatatta agtcttagta gttctaaaat
     1141 ccggaggcta aaatggataa taatacattt tataaacagg ataagattct tcacagaatt
     1201 ctctatacag ccaatatgtc caaaagaagt tctacacctg ccgatatttc ctctgatctt
     1261 ttaaaaatac tattcgagga aatgaaggcc accagggtca tcttctatca gatactccag
     1321 aaaaaacaga gtataaaaaa gcttgtttcc tttgaaacag annnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nctgacttca atggagaaaa aggttaagga aagcgaaaga
     1501 aagtaccaga agcttttctc aaatatgacc cagggagtga taatacttaa actcatacat
     1561 gattccgaaa aaaacctgaa agacttttcc atagaagaca taaactctgt agctaaaaaa
     1621 acttttaaaa atactgctct gttaaaaaac aaagaatgtg atttttttac gcctgaggag
     1681 aaaaaacttt ttatggaaat actgaagaag gctgaaaaat caaaaaagac ctttaaaaca
     1741 gagattactc ttcaatccca aagagttatt gatcttgtag catatatgat ttcaaaaaac
     1801 cagattgctt tcgttacaca ggatataact gcaaagaaaa agcttgaaga agaactaaag
     1861 acatatgcca gcatcgatga gcttactggt atctttaaca ggagagtggg tatatccctt
     1921 cttgaaaaag agatcatgca atcgcaaaga cgtaactctc ctctttcaat aatatatata
     1981 gacataaatg gtcttaaaaa caccaacgat agatttggcc actctatggg ggatgaactt
     2041 attaccaatg ccacaaaaat aataaaaaaa tgcgccagaa aatccgatat aatattcaga
     2101 atgggcggag atgaatttct tataatactg cctgactgta aagcagaaaa agcagaggtt
     2161 gtctggaaac gtatacgaaa tgaatgtgat cagttcaata aaaattcaga tacaccctat
     2221 aaaataagta tgagtcacgg aacagccacc tacagcggag aacaaacgat agatgaattt
     2281 gtcaaccaag ctgattctca gatgtacaaa gaaaaaatac tatacaagaa agaaacagaa
     2341 aaaagcagcg tttaaacgct gcttttgatt ttatgaactt attacttctg cgccatatac
     2401 ttaagaagat ccacaactct gttggagtat ccccattcat tatcgtacca tgaaaccact
     2461 ttaaagaatc ttttttcacc cttaagatta ttctgtaaag tagcgagaga gtcgtatata
     2521 gacgatctgt tgtcgtgaat gaagtcggtc gataccactt cttcgtcggc aactcccaga
     2581 taattctcaa gataagtctt ggaagctttt ttcagtagac tgtctatctc ttctattgaa
     2641 ctgtctcttg ctgctctaaa tgtaagatca acaacagaaa catctggtgt cggaactctg
     2701 aaggacatac cggtaagttt tcccttgact gccggaatta cttctcccac agcctttgca
     2761 gcacctgttg ttgaaggaat tatattgata gctgccgctc ttccgcctct ccagtctttt
     2821 ttggaaggtc cgtccaccgt cttctgagta gctgtatacg aatgaatcgt tgtcataaga
     2881 cctgtttcta tgccgattcc ttctttcatg ataacatgaa ccaaaggagc cagacagttg
     2941 gtagtacatg atgcattgga aactatatga tgtaacgaag gattatactc attttcgttt
     3001 actcccatta caatggtctt tacatcaccc ttacccggag ctgttattat aactttttta
     3061 gcgccagctg aaagatgagc ctttgccttt tcagattcgg taaaaagtcc tgtagactct
     3121 atgacatact ctacacccat ttcagcccaa ggcagcttcg caggaccttc ttttggggca
     3181 gccagacatt ttatcttatt gccatctaca acgagtatat catctgcttc aagtgaagaa
     3241 tcgcttttct gtgttgaaag cgcaccctta aactttccat gaatggagtc atatttaagc
     3301 tgataagcaa aataagatgc atcagtgctt acatcaacca cagcaaccac atcaaatttt
     3361 gaaccgagaa gcccctggtc gtataaagcg ttgaaaacca gtcttccaat tcttccaaat
     3421 ccgtttatag caacttttac cgccatgcta atcacctctt cacgcttaat tggtaaattt
     3481 aatcatcctc aagtataata tacatcaaac aggtgtactt tgtcaatttt ctgaatatta
     3541 ctgtgtttaa tttctttaaa tcacgtataa ttggattata cggcgaaatt tcgaaaaatg
     3601 cagttagtat gataaaattt atttggaaat aaactttata aggagggaaa aaatgtctca
     3661 gatttttgga gaaaatactg gaaaaggttt tataaaagag gaacctaacg atatttttgt
     3721 taaagctctt gaaagcgcca tatttaaagc cccggtcaga atggaggaaa aaatttttct
     3781 ttccgaccta tggataatta cttcacttcc ggaagatctt atggtaaata taatagaaaa
     3841 ccatgccgaa aaattaaata taccacagga catcaacttt atactggacg acaagaaaaa
     3901 caaaaaaata tgggaaagac ctgtttcaga aagggttgat aaacaccagt ctaagcctgc
     3961 ttcagacgaa gaagcaaaga aataaagaga ttttttgttc taattacttt tatttgttta
     4021 tatatcccat tatttagtta aaaacaactt ttttcgctct tgcatctgtc aagagataat
     4081 gttatgatat tcaatgtact tagcactctt gaaatcaaag tgctaacaaa tttaaaaaaa
     4141 tgtactatgt aatcaaatat ataaggaggg attcatgatg caagtgaaac cattaggaaa
     4201 gagagttctt ataaagccta taaccgaaga aaaaagaacc gaaggcggca tagttcttcc
     4261 agattcggca aaggaaaagc ctcagaaagc tttagttatt gccgtaggca accttgacgc
     4321 agagtatgat cttaaagccg gagataaggt tattttctca aaattcgccg gaaacgaaat
     4381 aaagattgaa gatgtagatc atatactgat tgatattgaa gatattttag gcaaagtagt
     4441 tgactaatta aatttacgga ggtgctttac atatggcaaa gatgttgaag tttgatgagg
     4501 aagctagaag atcactcgaa gccggagtag atgctgttgc taatgcagtt aagataactc
     4561 ttggcccgaa aggaagaaat gtagttcttg agaaaagctg gggttcacca actataacaa
     4621 acgatggagt ttccatagca aaagaaattg aactgaaaga taaatttgaa aatctcggag
     4681 ctcagcttgt aaaagaagta gcctccaaga ccaacgatgt agccggagac ggaacaacta
     4741 cagctacagt tcttgcacag ggaatgataa aggaaggtct taagaacgtt gccgcaggtg
     4801 caaatccgat gcttatgaag attggaatac agaaggctgc agacagagtt gtagaagaga
     4861 ttaaaaagat gagcagaaag cttaccggtt ctgaagacat agcaaacgtt gcagctatat
     4921 ctgcaaacaa tgaagaaatc ggaaatctta tagcagaagc catggataaa gtcggacagg
     4981 atggagttat caccgtcgaa gattcaaaga ccatggaaac ctacgttgaa tttacagaag
     5041 gtatgcagtt tgacagagga tacatctccc cttactttgt aaccaacgcc gacaagatgg
     5101 aatgtgaggt aaaagaaccg ttcatactta ttaccgacaa gaaaatttca agcgtaaagc
     5161 ctataatccc gatactcgaa aaagttgcac aggcaggaaa accgcttgtt ataatagctg
     5221 aggatattga aggtgaagca ttgtcaactc tggttctcaa caaactcaga ggaaccttgg
     5281 actcagtagg aattaaagca cctggtttcg gcgacagaag gaaagcaatg cttgaagata
     5341 ttgctatact taccggagga accgtaataa ccgaagacct cggtcttact cttgaaaacg
     5401 ctattattga cgatctcgga aaagctgacg ttataagaat aaagaaagac gacacaataa
     5461 ttgtaacagg caaaggcgat ccgaagaaga ttcaggaaag agtaaaacag ataaaggcac
     5521 agattgatgt aaccacatca gaatacgaaa aggaaacact ccaggaaaga cttgcaaaac
     5581 ttgcaggtgg cgtggcagta ataaaagtcg gtgcagctac cgaaaccgaa ctcaaagaaa
     5641 agaaacacag aatagaagat gcactttcag caacaagagc agcagtagag gaaggaatgg
     5701 ttgccggagg cggagttact cttctcagag ctgccaaggg tgtagaatcc ttgatagccc
     5761 agcttgaagg agatataaaa gtcggagcaa gagttgttct taagtcactc gatgcacctg
     5821 taagacagat agcgatcaac gcaggagtcg aaggcgcaat cataattgac agagtacttg
     5881 ccaaagatga tccttcatac ggatacgatg ctcttaaaga taaatatgta aatatgtttg
     5941 acgcaggaat aattgatcct accaaagtta caagaagtgc tgttcagaac gcagcttcaa
     6001 ttgccgctat gcttctgaca acagaagttc tcgtggtcga ggaacctaag ccagatgcac
     6061 cagcacctgc tccacagatg cctgaatatt gattaaataa gtaaagcgtg ccttttaaag
     6121 gcacgctttt ttatgatata attgccatat cggctcgtaa ggagggatat ttataaaaaa
     6181 aattattatt ctcatattta ttgttatttt ttgtattaat gcaggttttg catccgacac
     6241 aagaaagaaa attctctttt gccctgattt cagcggtatc tcatctgatg aacaggaagt
     6301 acttaaactt atagaagctc aggtaactga ttatatctat aaactcagaa agtacaggat
     6361 agttataaga gatcagacca tatatgacga agtaaaactg atgctttctg gtattaccca
     6421 gggaggatcg gagtatcagg ctcaactcga tacagacggt ataatatatg tccagatcct
     6481 tgatactttt tcagaacgtg ggaaaaacga ttccaaagaa tggatagact tccatgcaat
     6541 agtaaattat aaatttgtat atgtcaaaac tggagaagtc ctaaacagca gaaaaataga
     6601 tgcatactca agagcttata tagaaaagga tatttccgtt cagcaggcct tccgcacttc
     6661 aaaattatcg gcagttgact ttatatccca agagatatca aaagacatga gtcatgcttt
     6721 tattctttcg gcaaaggttt cgggtgagaa gtggctggat cttttttata tagatagtgg
     6781 aactaactat ggtataagcg aaaacatgac atttaacctt gttgaaaatc tcagcaatac
     6841 agcaggcaca tatgaagtca tcgaaggcaa agctgtagtg gtagaagctt ttccggattc
     6901 aagcatcata aaagtccttg aaaaacctct gcgctttcct atttcagcat caacaaccca
     6961 tttaatagaa aatccttcta taaaacttca aagaaaaagc tttagactct cggcttgctt
     7021 ggatggccta aaaattcccg gaatttttat aagaagcgga tatgacggct ataccggatt
     7081 gttttactca agttttatgc agacaggtct tcaagattat tcagtatcca tgaatctcgg
     7141 agtggacggc ggatatctca ttgatattaa cggccttctg aaaagcggag tttctatagg
     7201 ggcagcctgc ggatatgact ttcagaacaa ctccggaaaa aatttcaatc cttttactcc
     7261 ggaaagctca gatttctatc ttttcattcc attttgcgca tcattatatc ttaaaataaa
     7321 cgacagcctg tcctttgtat ccgatgtaac gtatcgtttc aatatatacg gtgacaaaaa
     7381 aattttcagc catgaaccat atgtcctaag agctggttta gagatatttt actgattttt
     7441 tggaaataca atgaacccgg ctttatactc tttggtatca tcactttcag caaactttga
     7501 atattcatgg aaaagctctg taagtttgaa cgtaaactct ttaaatttgg tttcatccag
     7561 atgcatggtt atataggaaa tgccagaatc cttgggaaat ttttcatgtg tttcctttat
     7621 ttttgataag tatgagtagt agtccctgtc catatttttg aataagttat caaaaatctt
     7681 aagggtttct gatttgaact ctttatcgtt agataaaagc tccttggaaa ttttaaaata
     7741 tctggcaaca ggttcataat acttctcaag tatccctgaa ttttcaaccg ttctgacaag
     7801 cctgagtatt ccgacactct ccatctcaat tatatgatag tatatctttg ccggagattc
     7861 cttcatttta tctgctatct gcttggctgt atatgcttta ccgctgaatt cctctattat
     7921 tcttattctt agaggatgag ctatggcctt tatagtagca atctcatcta aaatttttac
     7981 atcttccatt ttttcctccc aatataaaaa tgccgggctt gcccagcatt tatattatct
     8041 attatactct ctgacagttg ccttttttaa aatcccatga aaagctttat actttgagga
     8101 tcgtttaaaa taaattcaaa cgatgatttt acttcttttc tcagaatttc ataattctca
     8161 tctttccttc ctatcagttc tcttttcata cttcttctcc tttatactcc caagaaaatc
     8221 ttcataaggc tgctggacat gttcataagg ctgtattgat cattatgaaa ggcttttaga
     8281 tctcctatat catcatttct gaaacagaaa tcttcttttt tcataagcca cctccgtaat
     8341 gccggttaat aatgcatgga tgctttaaga gacatttaga cttatttttt tgattgtttt
     8401 ttatcgatta aaaaaattta atcatcaaat tcatttttat tatacaactt tcttttctca
     8461 agagtcaatt aattttttgc atcttatttt aatttatgat gttttatgtt aattttttga
     8521 aactccttgc tttttttaga atcactcttt ttacttcgaa agctttatac agctcttcat
     8581 ctatgctttg gattcaaagt atgctccata ctttctcatt acctattttg gctgtctgac
     8641 tgataattcc gcacgcatta agcgaatacg tcatctttct tgcagtactt atttttatac
     8701 ccagagcctg cgtaagctct ttatttgtaa atttactcgc tccttcaggc aaaaaatcta
     8761 aaaaatcttc ctttttctct attttttcag aaccttgcac actaagaagg aatctatcag
     8821 aaatacttac accttttctt ctgtagcttc ctttaccgtc atcaattctt acttcaagaa
     8881 gattgatata aatgagctca aacgaaaggt tgggatttcc tataaactgt gcaattctga
     8941 tagtctggtc aaaaagatca tatctgcagc cattttttgg cgacttcctt ttagaaataa
     9001 ctttcccgtc agtacttctt ttttctatgt acttacaaag aactatcgga taaacaactt
     9061 ttatcctata ctcacaaaga agatctttaa gtttatcttt cattgatgaa aactgcccaa
     9121 gctgcacttc tatgagaact cctttacggt atatatccgc aaagtatcct tttaccggaa
     9181 attcaagtat atcccccgga gtatagcagt atcttatgat ttccctgtga agagtgctct
     9241 cattaagcat tccaactttc tgcaccttaa taaccgcctt tctacctttc ttaagaaaat
     9301 acatttcatc ataaactttt ttacatatat tgattatata ctataattat atttagtttt
     9361 tcttttcact caggaggata taagatgata atattccata ttatcgataa aacagagctt
     9421 gagaacattg aaggttcaag atactacaag ccggcttcac ttgaaaatga aggatttatt
     9481 cactgttcca gaataaatca gcttgtaaga gttgcacaaa gtcattataa gcaaagaaac
     9541 ggaattttca taatggcgat tgaaagtaca gaagctggca gtcctgttgt ctttgaagat
     9601 ctttacaatc tgaatgaaaa gtatcctcat atatacggac cggttgaaat gactgcagta
     9661 aaaaacatat atcccatgga agaaaccgct gatggctttc ttctcccaga aaaagcagtt
     9721 attgaatgtt ctaaaaaaat ctttacttca gaaggtttcc aaagtcttat aacaagggca
     9781 gtgcccggag atactctcca tatcagtaac ttcatatctg acctattaat tgaaagacct
     9841 cttgaaaaag atctgcactt tagcgaagaa gatattctta atattaataa cagtgcggaa
     9901 agcattttat tgctttcacg caaggacggc cggcttgatg gtctgcttta tgtcaagcgt
     9961 aaaaataaaa aagatgtata catccgggcc ttgtaccgta ctgacttttc aggtgagtct
    10021 atgatcgctt atcttaaaga ttttgaaact cagaacggat gctatgatga taaagatctt
    10081 acattttata cttcaaacag cttcataaaa tctttgtgcg taaaacacgg tctcaagacc
    10141 gctaagccag acacaccttt ttccgctgat attccacata aggaggatga aaaataatgt
    10201 tccgtgttga tgcacatttt gatcttttat gctttgttta tgaaaaaaga gccaagggag
    10261 aaaccagagt aatcgagaag tatttttatg aagactggtt aaaaaacaac ttcaatatta
    10321 tagtttcctc aatcttcata gactctattt ttataccgga gatggcatta agaaatgcat
    10381 tggatcagat ttcatgttta tatgaggaaa tagctgaaac tcccgaaaaa atcatgctgt
    10441 gtaaaaatta catcgatatc gaaaaggcag aaaaggaaaa gaagatagga atccttatgt
    10501 ctctggaagg aagtgagcct attggaaacg atctttttct tctcagaact ttttatgagc
    10561 ttggagtacg gttcatcgga ctggcatgga acagaagaac ctactgtaca gacggtattc
    10621 ctgtatgcaa agatgtaaga aaacagtacg gcctaactga tcttggcata gatgttcttg
    10681 ctaaagccaa agaactcgga atgatagtag acgtaagcca ccttaccgac caaggttttg
    10741 aggacgtaat gacattttac gatatgcccg ttatcgcttc acactcaaac tgccggtctc
    10801 tgactggtca tttgcgtaat cttacggacg aacagatgaa aaaaattgca cagcgcggcg
    10861 gggttgtcgg gattaactct ttcagccttt tccttaatat ggaaccgctt aaagcatcta
    10921 tatcagatat ggccgagcat atgaaccata tgcgcagcat tatgggaacg gaccatatag
    10981 gattagggtt tgatttctgt tctcatataa ggagtagcct gtacgaaagt gaagaaaagg
    11041 acaaatttga tgccatgaac ggctacaatg aattttttat tttagataaa gaactttcaa
    11101 aaaaaggatt ctcacaagat gagcgggaaa aggtatattc aaaaaatttt cttcgcctgt
    11161 ataatcaaat tctgcaatga aaacagtaat ttaacatact gtatcttgaa aatttaataa
    11221 aatcagcatt accaacaaaa acagagattt ttcgatttat tttgctttgt acggttttat
    11281 tgtttaccag agtctttttc ttctgttaat atttttgatg tataattttc gtgagagatg
    11341 attcatattt ttctttttgt ttttttattc tcgttcagac caacattatt ggatatacag
    11401 aagtgttatt ttatataaat cgtgaagaaa ataactattt taatgtgaaa ttattcatat
    11461 aaggagggac cattgtggac tttaatctta ccaaagaaca gctgatggtt aaaaagatga
    11521 tacgagcttt cacagaaaac gaagtaaaac ctttggctgc cgaagttgat gaaacagaga
    11581 gatttccgca ggaaacagtg gataaaatga aaagatacgg acttttaggt atgtgcatgc
    11641 caaaaaatta cggaggagca ggcgccgatg aattaagcta tgcgatatgt gttgaagagc
    11701 tttcacgtta ctgcgctact accagtgtaa tattttcagc acataactcc ttatcctgct
    11761 ggccgattat gaaattcgga accgaagaac agaaacagaa gtatcttgtt ccgcttgcaa
    11821 agggagaaaa actcggagcc tttgccttaa cagaaccaaa cgccggaacg gatgcatctt
    11881 cacaggctac atcagcagtt aaagatggcg attactatat cataaacggg tcaaagatat
    11941 ttatcacaaa tggtggaaaa gctgatatat ttgtaatatt tgccatgact gacaaatcgc
    12001 ttggcacaaa aggaataact tcatttatcg tagaacgcaa cacacccggt ttttctatag
    12061 gaaaaattga aaaaaagatg ggtataagag gttcctcaac cgctgaactt gttttccaga
    12121 atatgagagt ccacaaggat aatatgcttg gaaaagaagg aaagggcttt tcgattgcca
    12181 tgcagaccct ggacggcgga agaataggca ttgcctctca ggctctagga attgcgcagg
    12241 gtgctttgga tgaaactata aaatacataa tgcagagaga acagtttggt aaatctctaa
    12301 gtaagttcca ggccattcag tttgatatag cagacatggt aacccgtgta gatgctgcaa
    12361 gatttcttgt atacagggca gcatgcgcca aacaggaagg gaaaccttat tctgtactgg
    12421 ccgcgcaggc aaagctattt gctgcagaaa cagctatgta cgtcaccaca aaagctgttc
    12481 agctccatgg aggttatggc tacaccaagg actaccctgt tgaacgtatg atgagagatg
    12541 ccaagattac cgaaatttac gaaggaactt ccgaagtaca gagaatggta atagccggaa
    12601 atacccttaa ataatttgat acgggaggtt tataaatgaa aataatagtc tgtataaaac
    12661 aggttccaga taccacggaa gtacgtatag atccggttaa aggtacattg ataagagacg
    12721 gagtgcccag tattcttaat ccggacgata acagcgcctt ggaagaagct ttgagaataa
    12781 aagaaactct cgggcaagat acaacggtaa cagtaataac catgggaccg ccacaggctg
    12841 aagaaatttt gagacaagcc atggctatgg gagcagacag cgcatatctg ttaagtgaca
    12901 gagcatttgc aggttctgac acatgggcta cttcaaacgc tattgcggca gctataaaat
    12961 ctattgataa ctttgatata gtattctgtg gaaggcaggc aattgacggc gatacagcac
    13021 aggtaggacc tcagatagcc cagagacttg gtatggcaca ggtaacatat gtacagtctt
    13081 taagggttga ggaaaactca atcatcgttg acagagcgct tgaagatggt tatgagacca
    13141 tacaagtcac tactccggtt cttctgactg ctatcaaaga gctcaacact ccaagatata
    13201 tgagcataaa cggaatattt gacgctttta gagaaaagga aattaaagtc ctgggtataa
    13261 aagatatagg acttgacccg gcattaaccg gattaacagg ttcacctact caagtaagaa
    13321 agtccttcac ccccgaaccc aagggtaagg gagaaatgct aaccggttct gtgaaagaaa
    13381 tgtccgacca gctcgttata aaacttaaac agaaacattt aatataagga ggcccgaaaa
    13441 atggaaagaa cacctgaata tctggctcaa tataaaaatg tctgggtatt tgctgaacaa
    13501 agaaacggaa agcttatgcc cgttgtcata gaactcatgg gtgaaggaaa aaagattgct
    13561 tcacagatag gtaccgaact ttgtgctgta ctcctcggat cagacgtaaa aaaattagcg
    13621 gatgaactta taagctatgg cgcagataag gtatatctat gtgaaagtga actttttaag
    13681 aactatacca ccgacggata ctacaaaacc atttcagaac ttataaatga atacaagccc
    13741 gaagtatttc tcataggtgc tacccatata ggaagagatc ttgctcccag aatatctgca
    13801 acagttgata ccggacttac ggcagactgt acaaagcttg aggtagatcc cgaggataag
    13861 aaacttcttc agaccagacc ggcttttggc ggaaatatca tggccactat agtgtgtcca
    13921 aagcacagac cccagatgtc aacagtaaga cccggcgtta tgaaaaaagc tgaaaaagat
    13981 tcttcaagaa ccggaaaaat aataactgta agtccgaaat taagctcaca ggatataaga
    14041 accaaggttt taaatatcgt taagatggtc aaaacaaatg ttgacataca acaggcagaa
    14101 attatagttg ccggaggtag aggtcttggc aaaaaagaag gctttgaact tattgaaaag
    14161 cttgccgaaa aagtaggcgg agtggttggg gcatccagag cagtagtaga cagccagtgg
    14221 ataactcagg atcatcaggt cggacagaca ggcaaaaccg taagacccaa ggtctatata
    14281 gcctgtggaa tatccggcgc catccagcat cttgccggaa tgcaggattc ggactgtatt
    14341 atagccataa acaaagaccc acaggcaaat atctttaaga ttgctgatta tggtattgtc
    14401 ggagaccttt atgaagtaat accacagatc atcaaagctc ttgataatat agatgaacta
    14461 agcaaatcag tttaggaggg atagtatgaa gtatgcagaa attccaagcg gatacgtctt
    14521 tgaagccaaa aaaataataa ccgcacacat ggtagatact tttgcacaga taaccggaga
    14581 taaaaatcct attcaccttg acgaagagtt tgcaaaaaca accgtcttcg gaagaagaat
    14641 agctcatgga atccttacca taggtcttgt ttctgaggtt cttggcatgg agttccccgg
    14701 aaaaggaact gtttatatga aacaggacgc caagtttctc aagccagtct atcttgatga
    14761 agagataaca gtaagagttg cagtaaagga aaagatagat gaaaagttca ggctggttct
    14821 taccacacag gttataaaaa gtgacggcca gcttgctgtt gatggcgagg cattggtaca
    14881 gttcaaagtt ctttaatttg aaccacaatc aaaaaaggca gaattttctg cctttttttg
    14941 cttacttaat taaaagtatc tgaattttca gatactttct ttttatgtcg tttttttttt
    15001 gatataatca tattatattt gattactctt attcttataa attctctttc ttaacgaggt
    15061 gtttgatgga aaagtatgca gccttttttg atctggacaa gactctttta gttaccaaca
    15121 gcgggaatct ctttttcaga tactgttacc ggctcgatca gataaagttc tgggaactta
    15181 taaagcttat cccctacccg cttttataca aactggatat gttgaacgtg gaagcattgg
    15241 ccagaaaact ggtgctgcgt ttcaaggggg cttccaaaga agaaatatat gaatttacag
    15301 gcatgtgggt aaaaaacatg gtaaaagact atttcagaaa atccgctgtt caggaactca
    15361 atcttcataa ggaaaaaggt gcacatacag taatgatatc agcctcaacc cacaatctgt
    15421 gctatcctat tcagaagtat ctaggcatag aagaactgat atgtacagat ataaagtttg
    15481 aaaataatat tttcacagga gatctgggcg tacactgtta tgcacaggaa aaactcacac
    15541 gggcagagaa tttctgtaaa aaccacggct acagtcttga ggatgcttac ttctacagtg
    15601 actcctactc tgacttgcat cttcttgaag aagtcggtca tcctgtatgc gtagatcctg
    15661 atatgattct aaaaaaaaca gccaaagaga aaaaatggcc tattctttac taaataaaaa
    15721 aactccccag ggagtttttt tatttttcat atttcttaat tgagtttttt ccgctttttt
    15781 ttgcagcgta aagtgcttca tcagcttttt taatacattc tctccaatca tcggaagcaa
    15841 cgttgcagta gtatcctatt gaaacactta taaacttttt gtcttcatat ctgaaaattc
    15901 tgctgctatt aatggtttgt ttgatttttt tacagaactg cacaacatcc ttttcgtctt
    15961 tcccgctcag aatatacaaa aactcgtccc ctccatatct tccggcaacc acgccctcct
    16021 tgtcataaga ccgtgagatc tttcctataa gctctataac ttcatcgccc ttttggtgac
    16081 catatgtatc gtttac
//
LOCUS       DAOSHR010000026        15852 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_3033_length_15852_cov_5.545230, whole genome shotgun sequence.
ACCESSION   DAOSHR010000026 DAOSHR010000000
VERSION     DAOSHR010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 15852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15852
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3033_length_15852_cov_5.545230"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="PLU28_02525"
     CDS             <1..298
                     /locus_tag="PLU28_02525"
                     /inference="COORDINATES: protein motif:HMM:NF022654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YjdF family protein"
                     /protein_id="HOG33975.1"
                     /translation="EYQVYELILTKLNSLYFSRGIFTEFKPEKEVNPKRMQRQISNSI
                     KSRGPGEKAFMAMKYEHELLKNERRKIKKVLEETENTEKFVKKQLRKKEKHRGH"
     gene            complement(301..762)
                     /locus_tag="PLU28_02530"
     CDS             complement(301..762)
                     /locus_tag="PLU28_02530"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOG33976.1"
                     /translation="MIKISEITSADKQIITADILKKLTQWFGIDSAINDYVSGVKETV
                     FFAAYEDEKPVGFISIKSNNEYTAEIYVMGIEKPYHRKGIGTILIEKAQLFLKNNGYR
                     FLMVKTLGESCDYECYARTRMFYKKCGFFPLQEIKEIWGEENPCLIMIKVL"
     gene            905..1219
                     /gene="trxA"
                     /locus_tag="PLU28_02535"
     CDS             905..1219
                     /gene="trxA"
                     /locus_tag="PLU28_02535"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOG33977.1"
                     /translation="MREITDATFETEVLKSELPVLVDFWATWCGPCRMIAPVVQSLSE
                     KYEGKVKFVKLDVDANPQNPQQYRVNSIPTLMIFKNGQPVDTSVGFKPESVIEKIIQK
                     NL"
     gene            1223..2095
                     /locus_tag="PLU28_02540"
     CDS             1223..2095
                     /locus_tag="PLU28_02540"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOG33978.1"
                     /translation="MEELFDIAIIGSGPAGLSAALNAKIRNKKILFLGKKELSEKLYK
                     AHTINNYLGFPSISGQDLKNSFKKHIDSLEIEIMDMFADTVYSMGDYFSISVKEKVFD
                     AKTVIVAVGMDFPKTIKGEREYLGKGVSYCATCDAMLFRNKKVAVIAYDKEDEAEALY
                     LSEIGCEVYYIPMYKETPKLSDRIKIINEKPLEISGSNFLSALITDQRSIEVSGVFVL
                     KQMKPVQDLIPGVETADGHIVVDRSMQTSIPGLFAAGDCTGKPYQYMKAAGEGLVASL
                     SAVAYLDKLNKNKV"
     gene            2254..2715
                     /locus_tag="PLU28_02545"
     CDS             2254..2715
                     /locus_tag="PLU28_02545"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOG33979.1"
                     /translation="MVMDGIDPHARKLMEVFSAIERSKWNDSPVKGLAANEAHVLFCI
                     KHKTIFPQEGMKVSEIGRLMKVTTPTITQTINNLYKKGLVLRIPDIHDRRSVRIKLTD
                     KGEEIIKRVSEHICITFNGLSQHLGSEESDKLAELLLKAHNYIINVKKIDI"
     gene            2732..4468
                     /locus_tag="PLU28_02550"
     CDS             2732..4468
                     /locus_tag="PLU28_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG33980.1"
                     /translation="MLKLFKNLKPYSLSVILVFTLIFVQVMAELYLPNLMSDIVDKGI
                     AKGDTSYIFKTGAFMLIVAAIGVGCMIAASFLSSRIATGFGRDVRKKLFTHIENFSLN
                     EFDRLSTASLITRTTNDITQVQMVLIVMMRMMAMAPMMCIGGIIMAVSKDPKLSLIIV
                     FAIPIVVGAILLVARKGIPLFKTMQKKIDKLNLILREELIGVRVIRAFNKQFDEQERF
                     NSSNKELTQTAIRVNQIMAFLMPFMMVIMNFITLAVIWFGAKRIDAMSMQVGDLMAFI
                     QYIMHIMFSLIMVSMMFILIPRASVSAARINEVLETSPEIKDPHGNKLETELQNQRGT
                     IEFRNVSFSYQGAAEPAIKDISFVANHGKTTAIIGGTGSGKSTIVNLIPRFYDVSGGN
                     IFVNGIDVKDYTQKELRDKIGLVPQNTMLFSGSISDNIRYGNPSATDEEVAYAAKIAQ
                     ADEFISSMPQKYDSQISQGGTNISGGQKQRLSIARALVRKPEIYIFDDSFSALDFKTD
                     AKLRKELALETVSSTVIIVSQRVSTVMNAEQIIVLEDGKIAGKGTHRALMENCPQYRE
                     IVASQLSEEELK"
     gene            4465..6345
                     /locus_tag="PLU28_02555"
     CDS             4465..6345
                     /locus_tag="PLU28_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG33981.1"
                     /translation="MSRSQTQDLQMKKPGGFGPKGSLGMPAEKPKNFRKTLRRLIKYL
                     SPYKINLIIIFSMAVLSTVFSILSPKIMGKATTKLFEGFILKVQGIAGAGIDFSYILK
                     ILAFLSVLYILSSFFSYVQQYVMAGVAQKTVYKMRKDISEKLARLPLKFYDSHAHGEI
                     LSRITNDVDNISNTLQQSITQLISSLVTLIGVIVMMLTISPIMTLITFVTLPLSFIIT
                     VAVAKKSQKYFAAQQKSLGQLNAHVEEMFSGHKIIKAYSREEKSLKEFEQINEELYSS
                     GWKAQFISGIIMPLLSFVSNIGYVLICVVGGVLASAKKLEIGDIQAFIQYSRQFNQPI
                     TQTANIANILQSTVASAERVFEILDEPEEESDESATDLINNVQGQVEFSNVTFGYSKD
                     SVLIKDMNIRVEKGQTVAIVGPTGAGKTTLVNLLMRFYDIGGGKITIDAQDIMSMKRN
                     SVRSIFGMVLQDTWLFSGTIKENIAYSNKDATFEQIVKAAQTAYADHFIRTLPQGYDT
                     LINEQANNISQGQKQLLTIARAVLADPKILILDEATSSVDTRTERLIQKAMIELMKGR
                     TNFVIAHRLSTIQGADLILVMNNGSIIEKGTHTELLEKKGFYAQMYYSQFTNSNSKDQ
                     AV"
     regulatory      6376..6442
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            6476..7663
                     /locus_tag="PLU28_02560"
     CDS             6476..7663
                     /locus_tag="PLU28_02560"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOG33982.1"
                     /translation="MLLTVGIIMVLGLATNFFFEKIGLPGLLGMLVLGIVIGPYGIDL
                     INHELLNFSADFRMFALIVILLRAGLGISRKELKKIGSPALRLSFIPGLLEGFTIALA
                     SVLLFGFSFVQGGMLGFILAAVSPAVVVPLMLKFSEQKRGTDKSIPTMIMAGASIDDV
                     FAITIFSAFLASYTNAGINVFKVLLNIPISIFLGIVTGAVAGLILVKVFSKYHMRDTR
                     KVIYILGLSILINGIESFLKSKVEIASLLGVMTIGYVILNRLPAAAVRLSQKFNKIWV
                     FAEILLFVLVGAQVNISLAFNSGLKGLVIILAGLLARSLGVYFSVMGTNLNFKEKLFC
                     ILSYTPKATVQAAIGAIPLSAGVENGDLILAVAVLAIMITAPLGAIAIKTGGEKLLSY
                     KQL"
     gene            7759..8991
                     /locus_tag="PLU28_02565"
     CDS             7759..8991
                     /locus_tag="PLU28_02565"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOG33983.1"
                     /translation="MLKDLFKKTIFPDDTKSAFTAQLLKENFHRSIILGYAVMLFESI
                     LITINLISYFGHVDTSYDFITYLLMYISMFTVNMLFVVMTKKVFLKIKWTESSMKMMS
                     GFQLGYVFFMIGWGIAITMLDQRLYGQITTFVVNVAVCAITFYMSGIKSFFIYIGALI
                     GLVTGLPFFQSNTDILIGHYVNISVFCFLAWIVCRMLYVNFHKNFTVNRELEKTNIVL
                     QTQYELNAMINKKLYELNKKLADLSIKDELTGLDNRRGLNEFLDNLLKTNTGKSIDLS
                     AIMIDIDFFKLYNDNYGHLKGDEVLKKIAGVIKTIVENNSDFTVRFGGEEFLIISYNT
                     DRQNFIKTADRIKQAVYDLGIVHQYSPIGKYVTVSLGVAFSRITVPQDINHLIEKADN
                     ALYVSKNDGRNKVTVVGG"
     gene            9018..10724
                     /locus_tag="PLU28_02570"
     CDS             9018..10724
                     /locus_tag="PLU28_02570"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HOG33984.1"
                     /translation="MKPKKIKEPLKKNINDPQLKNKLINSKSFLELFNNVQEAMIIHN
                     LHGKILAVNKQFLSMFKTDEERAFTLSIKDDLSSSTNSLDLLDGIWADVIIGNPKTFE
                     WTAKRPEDGFIFPVEVFLTKLSFSKKDVILASIKDITHYKSIQKEVEEKAIEVENFFN
                     LSPDLFCITDKNGVFVKNNIQWKVVLGYEQKDLKNMHLWDFAYEKNIPVIKEKINEAL
                     ASDKVIIFETNYRCKDQSLKYLEFRSTRYNSTIFTVALDITERKESERIIMDSKQRLE
                     MVLYGSEMAIWDWDIPSGKIIVNERWHKIVGIEEHKQAKSTITEWEKSIHFDDLKSFN
                     ERLNSHVQGQSDFFECEYRVLNSSGKWTWIQSKGKIVERDLLEKPMRMCGTHINITKR
                     KELEEKMFKMAHYDKLTGLPNRELFFERFSQSIVQHRRDKKIFALFFIDLDNFKYYND
                     SYGHEAGDRILVITAQRLLSSVRKSDIVARMGGDEFTIILRNISSIESASNVAAKIID
                     IFSKPFYIGDIESTIGISLGISIFPKDGENSEELIRKADTAMYSVKKTGKRGYRFYEE
                     NK"
     gene            10708..11142
                     /locus_tag="PLU28_02575"
     CDS             10708..11142
                     /locus_tag="PLU28_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG33985.1"
                     /translation="MKKTNNPPKKKWEDFTEVEFFTTIYPKMIESLNGCIEKKVAINN
                     IILLGFFSIVVATVVIFFINLSFLVLLVPVFWIWTKISKTIVIRKKNTEDMNRSLEEI
                     RLKRTKYIKKLYEYEDMPYIEFIESIKKELESFPQEMRSLTF"
     gene            11178..11535
                     /gene="ssrA"
                     /locus_tag="PLU28_02580"
     tmRNA           11178..11535
                     /gene="ssrA"
                     /locus_tag="PLU28_02580"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            11632..12627
                     /locus_tag="PLU28_02585"
     CDS             11632..12627
                     /locus_tag="PLU28_02585"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOG33986.1"
                     /translation="MNQYDIKMRLYRSILFVGALLSVICIIGNYLSSFPFWPNLKWIA
                     LFLTTSVSYWFSKNENYIKAFMFGFFFFLIWIFFPFAFLDSGGSNNNAIGYAFLLLIT
                     VTYLFSGWKRFFLVFSLIGIFMILHAVEFYFPQLITVYPAHTQFTDRMIQIPLLLFAS
                     FMIILKFAWEYEAVNIKLDDYAKFDELTGLYNRRMFNVEIEEALRNRKEEIFLVLLDL
                     DNFKKVNDRCGHIIGDEVLKKLGDYLRKFLGQDRNIVSRWGGDEFAIIYFGNRITLME
                     KLTHLKKEFKDYVSFYEKTTGISASIIELALYENVSQILIAADQELYKEKSRNSK"
     gene            12714..13079
                     /locus_tag="PLU28_02590"
     CDS             12714..13079
                     /locus_tag="PLU28_02590"
                     /inference="COORDINATES: protein motif:HMM:NF025161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4180 domain-containing protein"
                     /protein_id="HOG33987.1"
                     /translation="MKISIINSDGINIAMVENEDVLIKDAQSALELAVSIMYESDCTR
                     IIIRKELVAPDFFILSSRLAGDILQKYSNYKIKIAIVGDYSKYTSKPLKDFIYESNKG
                     KDFFFVSTVEEAIDKLRQK"
     gene            13252..13572
                     /locus_tag="PLU28_02595"
     CDS             13252..13572
                     /locus_tag="PLU28_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG33988.1"
                     /translation="MNADQVLRTLLESEKKAKEDLRAASQKFEEQYNDLWEKLQSDKD
                     EKLALASMQAKKMEKAAQTKAAEEMASAVNKINILKNKLEKMWNEREEFINTILNKFL
                     DDGK"
     gene            13569..14606
                     /locus_tag="PLU28_02600"
     CDS             13569..14606
                     /locus_tag="PLU28_02600"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HOG33989.1"
                     /translation="MKYPFLMAKSRALISNLLSRKDYENMMQLSSVDDVFSYLKNKTH
                     YGNTLSKTNFNSVHRKDLELLLKKTICLDLENLYFYIPEKAKEFLRCVIDRYELENIK
                     IIFGWLHSKRFPLEKEVILFPVFHKNYELSALVKNESFDAVMNFLKKTPYYIVIKAVY
                     ENYLKTKKLFILTNALDIWYYTRLFKLIKNLGKYADFLKKVFFMQCEFLDISSIYRCR
                     FLFGLSVNETKNFFLPIKEVSDIPDIHILLNMTNEKEFFDFLTEHDSSRYSIKTRNDL
                     FAQNIDRADERILLAYAKKTLKNLNNGFDVFSAYIYLREYEYRDLVTVIESVRYGIEP
                     QKAVRYLINGE"
     gene            14609..>15852
                     /locus_tag="PLU28_02605"
     CDS             14609..>15852
                     /locus_tag="PLU28_02605"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HOG33990.1"
                     /translation="MHEKIYPVTLISDKNNIESILKQLLDSGEFQSASAEFFLNEKVI
                     RELYQMDENPYEEIFQTFTYIFNEAEKQPEPKVEKIVFPTVLDTQEISEFTQELSMSI
                     TTIKKEILYRQKLKEDIKKNLDLMHSLDKLSIDLTELDNFRYLSYTIGKISRIYYQRF
                     VDSLEKENVLVLDLLEEKEFIWLFIIYECAQNTEKIISISNFLQTDLEISINGTASQI
                     SWEYWDKLRAIEYEIKILQLEIKKIFYEKRRFIYSYFDYIFVLKNIHDMLHEKCLLNN
                     NFFVISGWTTQKTYEKLLEKQKEEDLFIVLRSSCTEECPTKLKNPWFFKHFETLVTMF
                     GIPKSNEIDPTISISVLFLVFYGMMFGDIGHGLVLAFSGFILFLMKKNSIFFVIGSAG
                     LSSVLFGFLYGSFFGFEDIFPA"
BASE COUNT         5416 a         2553 c         2956 g         4927 t
ORIGIN      
        1 agagtatcag gtttatgaac tcattttaac caaacttaac tctctttact tcagtagagg
       61 tatctttaca gagtttaaac cagaaaaaga agtcaatccc aaaaggatgc aacggcagat
      121 aagcaacagc ataaaatcac gaggcccagg tgaaaaagcc tttatggcaa tgaaatatga
      181 gcatgaactg cttaaaaacg aacgtaggaa aattaaaaaa gttttagaag aaaccgagaa
      241 tacagaaaag tttgttaaaa agcagctcag gaaaaaagaa aagcatcgtg gacactgatt
      301 ttatagaacc tttatcatta taagacaagg attttcttct ccccatatct cttttatctc
      361 ctgcaatgga aaaaaaccac attttttata aaacatcctt gttctggcat agcattcata
      421 atcacaggac tcacctagag tctttaccat aaggaatcta tatccattat ttttgaggaa
      481 aagttgagct ttttcaatca aaatggttcc tatacctttt ctgtgatatg gtttttctat
      541 acccataaca tatatttctg cagtatattc gttgttgctt tttatggaga taaatccaac
      601 aggcttttca tcctcgtatg ctgcaaaaaa gactgtttct tttactcctg atacataatc
      661 gtttatagcg gagtcaattc caaaccattg ggttagtttt tttaggatat ctgctgtgat
      721 aatttgctta tccgctgaag tgatttcaga aatttttatc atatattttg tccctcctta
      781 tttataaatt ataacattat ccttcaatta cctttttccg gacttgacat gacctttgga
      841 tatatgttat aataaaaata cctactaggg gtataatagt taaataaaat attggaggaa
      901 aaatatgaga gaaataaccg atgctacatt tgaaactgaa gttttaaaaa gtgagttgcc
      961 tgttctggtt gacttctggg caacctggtg tggaccgtgc agaatgattg cacctgtagt
     1021 acagtcttta tcagaaaagt atgaaggcaa agtcaagttt gtaaagcttg atgtagatgc
     1081 taatcctcaa aatccccagc agtacagggt gaacagcata cccacactga tgatcttcaa
     1141 aaatggacag cctgtagata catccgtggg attcaaacca gaatctgtga tcgaaaagat
     1201 aattcagaaa aatttatagg ctatggaaga actctttgat attgcaatca taggcagcgg
     1261 tcccgcaggg ctttcagcgg cattaaatgc aaaaataaga aataaaaaaa tactattttt
     1321 aggaaaaaaa gagttgagcg aaaaacttta caaagctcac accattaata attatctcgg
     1381 ttttccaagt atatcaggcc aagaccttaa aaatagtttc aaaaaacata ttgactctct
     1441 tgaaatagag ataatggata tgtttgccga cacagtttac tctatgggag attatttttc
     1501 catatctgta aaagaaaaag tttttgatgc aaaaacggtt attgttgctg ttggaatgga
     1561 ctttcctaaa actatcaaag gtgaaagaga gtatctcggc aaaggcgtca gttactgcgc
     1621 aacatgtgat gcaatgcttt ttagaaataa aaaagtggca gtaatagctt acgataagga
     1681 agatgaagct gaagcccttt atctttccga aataggatgt gaagtttatt atattcctat
     1741 gtacaaggag accccaaaat tgtcagatcg gataaagatt attaatgaaa aacctctaga
     1801 aatatctgga agtaattttt tatccgctct tataaccgat caaagaagta ttgaagtttc
     1861 gggagttttt gttcttaaac agatgaagcc ggttcaggat ctaataccag gagttgaaac
     1921 tgcagatgga catattgttg ttgacagaag tatgcaaaca tctattcctg gcctttttgc
     1981 agcaggagac tgtacaggaa aaccttatca gtacatgaaa gcagccggtg agggtcttgt
     2041 agcttcgctc agtgctgtag cttatctcga taaacttaat aaaaataaag tataaaaacc
     2101 agcatcgtat atggtgctgg tttttattta cgatatagtc ttattagtga aaagaaaata
     2161 tgttgaaatg tcatataaac agaaatataa ttaggtatca taataatatt ttatttttat
     2221 tattatgata cctaattact tttagaggtg ataatggtga tggacggtat agatcctcat
     2281 gcaagaaagc ttatggaagt attttctgca attgagagat caaaatggaa tgacagtccc
     2341 gttaaggggc ttgctgcaaa tgaggcacat gttcttttct gtattaagca taaaacaatt
     2401 ttccctcagg aaggaatgaa agtatctgaa ataggcagat tgatgaaagt aaccacgcca
     2461 acgataaccc agactatcaa caatctttac aaaaaaggac tggtgctaag gattccagat
     2521 atacacgaca gaagatcggt cagaataaaa ttgaccgaca agggagagga gataataaaa
     2581 agagtatctg aacacatatg cattactttt aacggtcttt cacagcatct tggttctgaa
     2641 gagagcgata aactcgccga acttcttctc aaagcccaca actatataat aaacgtcaaa
     2701 aaaatagata tctaaacggg agaggattat attgcttaaa ctttttaaaa atctcaaacc
     2761 atattctcta tcagtaattt tagttttcac actgatattt gtacaggtta tggccgaact
     2821 ttatcttccc aaccttatgt ctgatattgt ggataaaggc atagcaaaag gcgatacatc
     2881 ttatatcttt aaaaccggag cttttatgct catagttgca gctatcggag ttgggtgcat
     2941 gattgcggca agctttcttt cctcacgtat tgctaccggt tttggacgcg atgtgcgaaa
     3001 aaaactcttt acacatatag aaaatttttc cttgaacgag tttgacagat taagtacagc
     3061 atctctaatt acaagaacca ctaacgatat aacacaagtc caaatggttc ttatcgtaat
     3121 gatgagaatg atggccatgg cgcctatgat gtgcataggc ggaataataa tggctgtgtc
     3181 caaagatcca aagctttcac ttataattgt atttgccata ccgattgtag taggcgccat
     3241 tcttctggtt gcacgtaagg gaataccttt atttaagact atgcagaaaa agattgataa
     3301 gcttaatctt attttaaggg aagagcttat aggtgtaaga gtaatacgtg catttaacaa
     3361 acaatttgat gaacaggaac ggtttaattc ttcaaacaag gagctcactc agacagcaat
     3421 aagagtaaac cagataatgg cttttctgat gcctttcatg atggttatca tgaacttcat
     3481 tacattggct gtaatatggt ttggtgcaaa aaggatagat gccatgagca tgcaggtggg
     3541 agacttaatg gcatttatac agtacataat gcacataatg ttctcactta tcatggtttc
     3601 aatgatgttt attcttattc ctcgggccag tgtttcagca gcaagaatta atgaagtgct
     3661 tgaaacatct cctgaaataa aagatcctca tggaaataag ctggaaactg aactgcaaaa
     3721 tcaaagagga actattgaat ttcgaaacgt ctccttttca tatcagggtg ccgccgaacc
     3781 tgcaataaag gatatttctt ttgttgctaa ccatggaaaa actacagcta taataggagg
     3841 aacaggaagt ggaaagtcta caatagtaaa tcttataccg agattttacg atgtctctgg
     3901 cggaaacatt tttgttaatg gcattgatgt gaaagattat actcagaaag aactgagaga
     3961 taagatcggt cttgttcctc agaacactat gcttttttct ggcagtataa gtgacaacat
     4021 aagatatggc aatccgtcag caacagacga agaagtagcc tacgctgcaa aaatagctca
     4081 ggctgatgaa tttataagca gtatgccaca aaaatatgat tcacagatat ctcagggagg
     4141 aacaaacata tcaggcggac agaagcagag actatcaatt gccagagccc ttgttagaaa
     4201 gcctgaaatt tatatttttg atgacagttt ttcagctctg gattttaaaa cagatgctaa
     4261 actcagaaaa gaactggcct tggaaaccgt gagttcaacg gttatcatcg tatctcaaag
     4321 agttagtact gtcatgaatg cagaacagat aatcgttttg gaagatggaa agattgcagg
     4381 aaaaggaact catagagctt taatggaaaa ctgtcctcaa tacagagaaa ttgtagcttc
     4441 tcagctttcc gaggaggaat taaaatgagc cgcagccaga cacaggatct acagatgaaa
     4501 aaaccaggag gatttggacc gaaaggatct ttaggaatgc ccgcagaaaa acccaaaaat
     4561 ttcagaaaga cccttcgcag actaataaag tatcttagcc catacaaaat taatcttata
     4621 attatttttt ctatggcagt actaagcacg gtattttcaa ttctttcgcc taaaattatg
     4681 gggaaagcca ccacaaaact ttttgaaggc ttcatactga aagtacaggg tattgcaggt
     4741 gcgggaatag atttttctta tattcttaaa atccttgcat ttctttcggt tctctatatt
     4801 ttaagttcct ttttcagtta tgttcagcaa tacgttatgg caggcgttgc acagaaaacg
     4861 gtttacaaga tgagaaaaga catatcggaa aagttagcac gacttcctct taagttttat
     4921 gattcccatg ctcacggcga aattttgagc agaataacca atgatgtaga caatataagc
     4981 aatactcttc aacagagtat aacccaactc atatcgtcct tggtgactct cataggagtc
     5041 atagtaatga tgctgactat aagtcctata atgactctta ttacttttgt tactcttcct
     5101 ttgagcttta taataactgt agctgtagca aaaaaatctc aaaaatactt tgctgcacag
     5161 caaaaatctt taggacagct taacgcacat gttgaagaga tgttttcagg tcataaaatt
     5221 ataaaagcat actccagaga agaaaaatca cttaaggagt ttgaacaaat taatgaagaa
     5281 ctttacagca gcggctggaa agcacagttt atatctggaa taataatgcc tcttctttct
     5341 tttgtaagca acataggata tgttttaata tgtgttgtcg gaggagttct agcatctgca
     5401 aaaaaacttg aaattggaga tattcaggct ttcatacagt actcaagaca gtttaaccag
     5461 cccataactc agacagccaa tattgcaaat attttacaat cgacagttgc ttctgccgaa
     5521 agagtttttg aaatacttga cgaacctgaa gaagagtcag atgaaagcgc tactgatctt
     5581 atcaataacg tacaaggaca ggtagagttc agcaacgtta cttttggata ttcaaaggat
     5641 tctgttctta tcaaggatat gaatatacgg gtggaaaaag gtcagactgt agctatagta
     5701 ggacctacag gagccggaaa aaccacgcta gtaaatcttc tcatgaggtt ttacgatatc
     5761 ggcggtggaa aaataactat agacgcacag gatataatgt ctatgaaacg caactccgtc
     5821 agaagcatat tcggaatggt gcttcaggat acatggcttt tcagcggcac tataaaagaa
     5881 aatattgcat attcgaataa ggatgcaacc tttgagcaaa tagtaaaagc ggctcaaacc
     5941 gcttatgctg atcattttat aagaactctc ccacaaggct atgatacttt aattaacgaa
     6001 caggctaata acatttcaca gggtcaaaaa cagctgctta ctatagcacg ggctgttctt
     6061 gccgatccga agattcttat attggatgaa gccaccagca gtgtagatac aagaacagaa
     6121 agacttattc aaaaagctat gattgaactt atgaaaggaa gaactaattt tgttatcgct
     6181 cataggcttt caactattca gggagcagat ctcatacttg tcatgaacaa tggttccata
     6241 attgaaaaag gaactcacac agaactcctt gagaaaaaag gtttttatgc ccagatgtac
     6301 tacagtcagt ttactaattc aaattcaaaa gatcaggcag tatgacttga cactatttac
     6361 ggcatatgat aaaattgccg cagtggtgat ggagttcgcc cttaaacgca tggaataaaa
     6421 tgctgatgac tcctgtcgga actccggcag gagtcattat tttttggagg aaacaatgct
     6481 tttaactgtt ggaataataa tggttttagg tcttgcaaca aacttttttt tcgaaaaaat
     6541 tgggcttcca ggacttttag gcatgcttgt gcttggaata gtcatcggtc cttacggcat
     6601 agacttgata aatcatgaac tgcttaattt ttcagctgat ttcagaatgt ttgctcttat
     6661 tgtcatactt ctaagggctg gactgggcat cagcagaaaa gaacttaaaa aaatcggctc
     6721 ccccgcattg cgcttaagct ttatcccggg tcttttagaa ggttttacca tagccttagc
     6781 ttctgtttta ttgtttggct tttcttttgt tcaaggcgga atgcttggtt ttattcttgc
     6841 agctgtttct ccggcagttg ttgtaccgtt gatgcttaaa ttctcagaac agaaaagagg
     6901 cactgacaag tccataccca ccatgataat ggccggagct tctattgatg atgtttttgc
     6961 aataactata ttttcggctt ttttagcatc ttatactaat gctgggatca atgtttttaa
     7021 agtacttctc aacattccta tttcaatatt tttaggaata gtaaccggcg ctgtggcagg
     7081 attaattctt gtaaaggtgt tttcaaagta tcacatgcgt gataccagaa aagtcatata
     7141 tatattaggt ctttctattc tgattaatgg catagagagt ttcttaaaga gtaaagttga
     7201 gatcgccagt ctccttggtg taatgactat tggttatgtt attcttaata gacttcctgc
     7261 tgctgcagtc agactttccc agaaattcaa taaaatatgg gttttcgctg aaatactact
     7321 gtttgttctt gtcggagcac aggttaacat cagtcttgct ttcaactctg ggttaaaagg
     7381 cctggtcata atccttgcag gtcttctagc aagaagtcta ggtgtttact tctctgttat
     7441 gggaactaac cttaatttta aagaaaagtt attctgtatt ctgtcttata ctccaaaagc
     7501 gacggttcag gcagcaatag gtgccattcc tctttccgca ggagtagaaa acggcgatct
     7561 gatactggct gttgccgtgc tggctatcat gattactgct ccgcttggtg caatagcaat
     7621 taaaacagga ggagaaaaac tgctttcata caagcaactt tgaaactgct aaaagttaca
     7681 ttctctaatg tataatatga atgagtaagc ttacacataa atgagtaacc ttacatttat
     7741 ttattttgga gttgctttat gctaaaagat ctatttaaga aaacgatatt ccccgatgac
     7801 acaaaaagtg cgtttactgc tcagctgctt aaagaaaatt ttcatagaag tataattcta
     7861 ggatatgctg taatgctttt tgaaagtatt ctgataacta taaatcttat atcctatttt
     7921 ggacatgttg acacatcata tgattttatt acctatctgt taatgtatat aagtatgttc
     7981 acggttaata tgctttttgt agttatgact aaaaaagttt ttttaaaaat caaatggacc
     8041 gaatcatcta tgaaaatgat gagtggcttt cagcttggtt atgtcttttt catgataggc
     8101 tggggaatag caataactat gcttgaccag agactttacg gacaaataac tacatttgtg
     8161 gtaaatgtgg cagtatgcgc tataactttt tatatgtccg gcataaaatc tttttttatt
     8221 tatattggtg cacttattgg ccttgtaaca ggtcttccgt tctttcagtc caataccgat
     8281 atcctcatag gtcattatgt taacataagc gttttttgtt ttcttgcatg gattgtatgc
     8341 agaatgttat atgtaaattt tcacaaaaac tttaccgtta acagagaact tgaaaaaacc
     8401 aacatcgttc ttcagactca gtatgagctt aatgctatga taaacaagaa actttatgag
     8461 cttaataaaa aacttgcaga tctttctata aaggatgaac ttacaggtct tgacaacaga
     8521 cgtggcttaa atgaattttt ggataattta ttaaaaacca acactggtaa aagcattgat
     8581 ctttcagcca taatgattga tatagacttt tttaaactat acaacgacaa ctatggacat
     8641 cttaaaggtg acgaggtatt aaaaaagatt gccggagtta tcaaaactat agtcgaaaat
     8701 aattcggatt ttactgtccg attcggtgga gaagagttcc tgatcatttc ttacaatact
     8761 gacagacaga attttattaa aaccgcagat agaatcaaac aggcagtata tgatcttggg
     8821 attgttcatc aatactctcc gataggcaaa tatgttaccg tctctctcgg agtagctttt
     8881 tcaaggatta cagttccgca agacataaac caccttattg aaaaggccga taatgcttta
     8941 tatgtatcaa aaaatgatgg aaggaataaa gttaccgtag ttggtgggta atccttccag
     9001 ctaaaaggga gaaacttatg aagccaaaaa aaatcaagga gcctctcaaa aaaaatatta
     9061 acgatcccca actaaaaaac aaattaatta attcgaaaag ttttcttgaa ctttttaaca
     9121 acgttcagga agctatgatt atacacaatc ttcatggaaa aatacttgca gtcaataagc
     9181 agttcctttc aatgtttaaa acagatgaag aaagggcatt tacgctttcg attaaagacg
     9241 atctttcttc ttctaccaac agcttggatc tgctggatgg aatttgggct gatgtaatca
     9301 tcggtaatcc taaaactttt gaatggactg ccaaaagacc agaagacggt tttatattcc
     9361 cggtcgaagt atttctgacc aaattaagtt tttcaaaaaa agacgttatt cttgcttcaa
     9421 taaaagatat tacccattat aaaagtattc agaaggaagt agaagaaaaa gccatagagg
     9481 tagaaaattt ctttaatcta tccccagatc ttttttgtat aacagataag aacggcgttt
     9541 tcgtaaaaaa caatatccaa tggaaagttg ttttgggtta tgaacaaaaa gatcttaaaa
     9601 atatgcattt atgggatttt gcttatgaga aaaatatacc tgtcattaaa gaaaaaatta
     9661 atgaagcatt agcttctgat aaagtaatca tctttgagac caactaccgt tgcaaagatc
     9721 aaagcttaaa gtatctggag tttagatcta caaggtataa cagcactata ttcactgttg
     9781 cccttgacat aacagagcgg aaagaatcag aacgtataat aatggacagc aaacaaaggc
     9841 ttgaaatggt tctttacggt tcagaaatgg caatatggga ctgggacatt ccttccggaa
     9901 agataatagt aaatgaaaga tggcataaga tagtaggaat agaagaacat aaacaagcta
     9961 aaagtactat aaccgaatgg gaaaagtcaa tccattttga tgatcttaaa agttttaatg
    10021 aacgtttgaa ttctcacgta cagggacaat ctgacttttt tgaatgtgag tacagagtcc
    10081 ttaacagctc aggaaaatgg acttggattc agagcaaagg taagatagta gaaagggatc
    10141 ttttagaaaa acctatgaga atgtgcggaa ctcatattaa tataaccaaa agaaaagagc
    10201 ttgaggaaaa aatgtttaaa atggctcatt acgataaact gacagggctt ccaaacaggg
    10261 aacttttctt cgaaaggttt tctcagagta ttgtacaaca caggcgtgat aaaaagatct
    10321 ttgcactctt ctttattgat cttgataact ttaaatacta taatgattct tacggtcatg
    10381 aagccggaga tagaattctt gtcattaccg cacagcgtct tctcagtagt gtacgcaaat
    10441 cggatatagt tgccagaatg ggaggagatg aattcaccat tattctcaga aatatatcaa
    10501 gcatagaaag tgcttccaat gttgcggcta aaattattga tattttctca aagcctttct
    10561 atataggcga tattgaaagt actataggta tttctttagg aatatctatc tttcccaaag
    10621 atggcgaaaa cagcgaagaa cttatccgaa aagccgatac tgccatgtac tctgttaaaa
    10681 aaacgggaaa aagaggttat agattttatg aagaaaacaa ataatccccc taaaaaaaag
    10741 tgggaagact ttactgaagt agagtttttt actactattt atcctaagat gatagaaagt
    10801 ctaaatggat gtattgaaaa aaaagtggct atcaataata tcatactttt aggttttttt
    10861 tcaattgttg tagctactgt ggtgattttt tttattaatc taagttttct tgtactgttg
    10921 gtgccggttt tttggatctg gacaaaaatc agtaaaacta ttgttatccg taagaagaac
    10981 acggaagata tgaacagatc cctcgaagag atcagactca aaaggacaaa atatattaag
    11041 aaactttatg agtatgagga tatgccttac atagaattta tagaaagtat aaaaaaagag
    11101 ttagagagtt ttcctcagga aatgcgtagc ttgacttttt gaatcattat atgataaaat
    11161 atgatgccct tgaaaagggg ggcgtaccgg attcgacggg gtttggctat tacaagtagc
    11221 gagccgggga agtcattagc ccgttaacaa aatgacaaag aagaaacgca gaagaaaatt
    11281 acgcatttgc tgcttagttt aatctaacag ccgtcctgca gattaacctt gggcttgatt
    11341 aactgctagg gcgtgtctaa aaaggccctt acctgtcagt tttttctttc ggactgattg
    11401 ggaaatcaaa gaaagatagg atgagagatc ctgcctgcag gagttcgtaa tccgaaatca
    11461 aaatacaggc tgtgctcgga gaagcttgtc ttaatcactc ttcggacgcg ggttcaactc
    11521 ccgccgcctc caccattagg acgcagttgc atattcattg cctgcgtctt tattatttac
    11581 ggtctcgttt acagcaggac catatccata aattattctt ttggggtgag tatgaaccaa
    11641 tatgatatta aaatgcgtct ttatcggtca attttatttg ttggtgcact tttatctgta
    11701 atatgtatta taggcaacta tctttcatcc tttccttttt ggcccaatct taaatggata
    11761 gcattatttc tgactaccag tgtttcttat tggttttcaa aaaatgaaaa ctacattaaa
    11821 gcattcatgt ttggcttttt tttctttctc atatggatct ttttcccatt tgctttcttg
    11881 gattctggcg gaagtaataa taatgctatt ggatatgctt ttcttcttct cataacagta
    11941 acttaccttt tcagcggatg gaaacgtttt ttccttgtat tttctcttat tggcatcttt
    12001 atgatattac acgccgttga attttatttc cctcagttga ttacggttta ccccgcccat
    12061 acacagttca cagacagaat gattcagata cctttattgc tgtttgcttc atttatgata
    12121 attttgaagt ttgcatggga gtatgaagct gtaaacataa aactggatga ctacgccaaa
    12181 ttcgacgaac ttacgggact ttataacaga cgaatgttta atgtagagat agaagaagcc
    12241 ttgagaaaca ggaaggaaga aatatttctt gttctgctgg atcttgataa tttcaaaaaa
    12301 gtaaacgaca gatgtggaca tattatcggt gatgaggtac ttaaaaagct tggagattat
    12361 ttacgtaaat ttctagggca agacagaaac attgtgagca ggtggggcgg agacgaattt
    12421 gctataatat acttcggaaa cagaataact ttgatggaaa aactcacgca tctcaaaaaa
    12481 gagtttaaag attatgtatc tttttatgag aaaacaaccg gaataagtgc gagcattata
    12541 gaattggctt tatatgaaaa tgtctctcaa atacttatcg ctgcagacca ggagctttat
    12601 aaagaaaagt ctagaaacag caagtaatcc aaaaagtttc ttttcaatat gaaaaacatc
    12661 aatattatta taaaatgatc ttatatttaa tctataaacc atgggagcaa ttaatgaaaa
    12721 tcagtattat aaattcagat ggaatcaata tagcaatggt tgaaaacgaa gatgtcttaa
    12781 taaaagatgc tcagtccgct ctggagcttg cggtaagtat tatgtatgaa tcagactgta
    12841 ccaggataat tattagaaaa gagttggttg cacctgattt ttttattttg agttcgcgcc
    12901 ttgcaggaga tattcttcaa aaatacagca attataagat aaaaatcgca atagtgggag
    12961 attattctaa atataccagt aagccactca aagacttcat ttacgaaagc aacaaaggaa
    13021 aggatttctt ttttgtatca actgttgagg aagcaattga taaacttaga caaaaataag
    13081 tatacactat cttcaagaaa gaacagcatt ttcatataat cctgaatttt ttcaggattt
    13141 tttttattgt tgataatatt taatgatcaa tatttattat aaataaaaaa ttacatttgc
    13201 cttttgttgt agttacgtta tataatttat agtaaaaggg gggacataat tatgaatgca
    13261 gaccaagtac tgagaacgct ccttgaaagc gaaaaaaaag cgaaggaaga tcttagggct
    13321 gcctctcaaa agttcgaaga acagtataat gacctttggg aaaaacttca atctgataaa
    13381 gatgagaagt tggcattggc cagcatgcag gcaaagaaaa tggaaaaagc tgcacaaacc
    13441 aaagctgcag aagaaatggc ttcggctgtt aataaaataa atattcttaa aaataagctt
    13501 gaaaagatgt ggaatgaaag agaagagttt ataaatacaa tattaaataa atttttagat
    13561 gatggaaaat gaagtatcct tttttgatgg ctaaatcaag agctttaatt tcaaaccttc
    13621 tttcaagaaa agattatgaa aatatgatgc agctttcatc agtagatgac gttttcagct
    13681 atctgaagaa taaaactcat tatgggaata ctcttagcaa aacaaatttt aactctgtac
    13741 atagaaaaga tctggaactg ttgctaaaaa aaactatttg tttagatctt gagaaccttt
    13801 atttttatat tcctgagaaa gcaaaagaat ttctaagatg cgtaatagac agatatgaac
    13861 ttgaaaacat taaaattatt tttggatggc ttcactcaaa aaggtttccg ttagaaaaag
    13921 aagtaattct attccctgtt tttcataaaa attatgagct ttctgcttta gtaaaaaatg
    13981 agagttttga cgcagttatg aattttttga aaaaaactcc atactatatt gttatcaaag
    14041 ctgtttatga gaattatttg aagacaaaaa aactttttat actgactaat gctcttgata
    14101 tctggtatta tacaagactt ttcaaactta taaaaaacct tggtaagtat gcagattttt
    14161 taaaaaaagt tttttttatg caatgcgaat ttcttgatat atccagtata tatagatgta
    14221 gatttctttt cggtctttct gtgaatgaaa ccaaaaactt ttttcttcct ataaaagaag
    14281 tatctgatat acccgatatt catattctac ttaacatgac taatgaaaag gagttctttg
    14341 attttctaac cgagcatgac agttcacggt attctataaa gaccagaaat gatctatttg
    14401 ctcaaaacat agacagagct gatgaaagaa ttcttcttgc atatgcaaaa aaaacactta
    14461 aaaatctgaa caacggtttt gatgtattca gtgcttatat ctatcttaga gagtatgagt
    14521 acagagatct tgttacagta atcgaaagtg tacggtatgg tatagaacct caaaaggctg
    14581 tcagatattt aattaatgga gagtgaaaat gcacgaaaaa atttatcccg ttacactgat
    14641 ctcagacaag aacaatattg agagtatctt gaaacagttg cttgacagtg gagaatttca
    14701 aagtgcatct gcggagtttt ttctgaatga aaaagttatc agagaacttt atcaaatgga
    14761 tgaaaatcca tacgaggaga tatttcagac atttacatat atattcaacg aagcagaaaa
    14821 acagccggaa cccaaggtag aaaaaattgt ctttccgaca gttcttgata cgcaggagat
    14881 atcagagttt acacaggaac tatccatgag tataacaact attaaaaaag aaatactgta
    14941 caggcaaaaa cttaaggaag atatcaaaaa gaatttagac cttatgcata gtctggataa
    15001 actaagtatt gatctgactg aactggataa ctttcgatat ctttcttaca ctataggcaa
    15061 aatatcaaga atttattatc aaagatttgt tgatagtctt gaaaaagaaa atgtattagt
    15121 tttagatctg cttgaagaaa aagaattcat atggcttttc ataatatatg aatgtgcaca
    15181 aaatacagaa aaaattattt caataagcaa ttttttgcag acagatcttg agatatctat
    15241 taacggtact gcttcacaga tatcctggga atattgggat aaattacggg caatcgagta
    15301 cgaaataaaa attttgcagc ttgaaattaa aaaaattttt tatgaaaaaa gacggtttat
    15361 atacagttat tttgattata tctttgtact aaaaaacatt catgatatgc tacatgaaaa
    15421 atgcttgctt aacaacaatt tttttgttat ctcaggctgg actactcaga aaacatatga
    15481 aaagctttta gaaaaacaaa aagaagaaga tttgtttata gttcttagat cttcatgtac
    15541 tgaagagtgt ccgacaaaac ttaagaatcc ctggtttttc aaacattttg aaactcttgt
    15601 tacaatgttt ggaattccca agagcaatga gatcgatcct acaatttcta tctccgttct
    15661 tttccttgtt ttttatggaa tgatgttcgg agatatagga cacggacttg ttttggcatt
    15721 cagtggattc atactttttt taatgaaaaa aaatagtatt ttttttgtta taggaagtgc
    15781 cggcttatct tctgtgctat ttgggttttt gtatggaagt ttttttggat ttgaagatat
    15841 cttccctgcc tt
//
LOCUS       DAOSHR010000027        15018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_3252_length_15018_cov_5.687964, whole genome shotgun sequence.
ACCESSION   DAOSHR010000027 DAOSHR010000000
VERSION     DAOSHR010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 15018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15018
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3252_length_15018_cov_5.687964"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..142)
                     /locus_tag="PLU28_02610"
     CDS             complement(<1..142)
                     /locus_tag="PLU28_02610"
                     /inference="COORDINATES: protein motif:HMM:NF012361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOG33991.1"
                     /translation="MVKKFYVTTPIYYVNSEPHIGSSYTTIVADIIARYKRMMGFDVF
                     FLT"
     gene            255..1988
                     /locus_tag="PLU28_02615"
     CDS             255..1988
                     /locus_tag="PLU28_02615"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOG33992.1"
                     /translation="MRFSKLYAPTLKEAPADADIKSLELLIRGGFIRKVAAGVYTYLP
                     LGFRVIKKIEEIVRQEMESIQCQETMMPIIQPAELWKTTGRWDDYGPEMMKLNDRHNR
                     DFTLGPTHEEMITYIVKNELRSYKQLPISLYQIANKYRDEIRPRFGVLRAREFIMKDA
                     YSFHTSQESLDESYKDFYSAYERIVKRLGAAYSIVEADSGAIGGSGSHEFQVLAKTGE
                     STIFYCDECGYSATDEKATAITEFSSQNEEKKSIELKDTPNVKTIEQVAHFLNVPLEK
                     IVKSLLLKGRNGWVLALIRGDYEINLSKVRAVFQDQTIDMGDPMDIKNTFGVEIGFIG
                     PVGLKGPQIIADDSVKPLMNFVVGGMEKDKHFINANIGRDFTVGKYSDLRVVKAGENC
                     PHCKKPLKQARGIEVGQVFKLGTKYSSSMQAYYTDETGNPHPYIMGCYGWGISRTMGA
                     VVEQLHDENGILWPVSIAPYKIAVVPVSMSDTAMVQTAEKIYSALLEKNIDVILDDRT
                     ESPGFKFKDIDLIGIPLKIIAGKGVKEGKVEIKLRNEKQGFMLSIDDIDKIVDFAIEK
                     LNGYNPTSILD"
     gene            2052..2140
                     /locus_tag="PLU28_02620"
     tRNA            2052..2140
                     /locus_tag="PLU28_02620"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2086..2088,aa:Ser,seq:gct)
     gene            2155..2231
                     /locus_tag="PLU28_02625"
     tRNA            2155..2231
                     /locus_tag="PLU28_02625"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2189..2191,aa:Arg,seq:gcg)
     gene            2338..3600
                     /locus_tag="PLU28_02630"
     CDS             2338..3600
                     /locus_tag="PLU28_02630"
                     /inference="COORDINATES: protein motif:HMM:NF019244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HOG33993.1"
                     /translation="MAGETYIDNEKRFFILKTYAFSFIIFGLLFSNYETFFDDIFRII
                     TEPDFLIVDYIKLSGIGAAFLNCGIVTMAAIMILKKAKVEITGVSIASLWTLSGFSLF
                     GKNIFNVWFVIIGVFIYSKIRKESFRKYVYIAFFGTSLSPIITQIIFSDALPSFLNLI
                     LAFITSTFLGFLLPALSSYFLSFHRGYNLYNVGFTAGIIATFLVSILRAYGYSFEPRI
                     LWSSGNNVLLGIYLYVLFLVMIYMGYRYNKNSFSGVKKISTYSGRLVTDFVLLEGMEK
                     TLINMGSVGLAATSYVLLTGAALNGPTIGGIFTIVGFGAFGKHLRNIIPIFLGVFLGS
                     ITNMYDIHDPNMVLAALFGTTLAPIAGEFGIFWGIIAGFLHSSLVTNLGYLHGGMNLY
                     NNGFSGGLVAAFLLPIIQAFERRGKDEH"
     gene            3590..3931
                     /locus_tag="PLU28_02635"
     CDS             3590..3931
                     /locus_tag="PLU28_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG33994.1"
                     /translation="MNTERIKVLRIANELLNYMFLRCSHALTLELKENILGFEITIRS
                     ACSHINSENIAELKKLLTPGRHEEISDYYWNLAGTNSEENELNLIGLMIDSAEVKAEN
                     NELVIKLVRKK"
     gene            4028..5299
                     /locus_tag="PLU28_02640"
     CDS             4028..5299
                     /locus_tag="PLU28_02640"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HOG33995.1"
                     /translation="MSATVHFPEGFLWGAATASHQIEGANYNSDWYEWEKKGKIKDNS
                     SSDPGCQSEKYYERDITFLSDLNLNAYRFSVEWAKIQPDENSFCENHLQRYCKIVDIL
                     LSKRIEPVVTLHHFVNPLWFYNKGAFEKPENISFFLKFVEKMVSAFKEKVQYYTVINE
                     PIVYAFTSYIDGSWPPGKKDWNAGMAVVRNLLVAYDEAYKIIKKINPNAKISVAKHTA
                     VYRPYLSFSPIDIIASRTIQRYFDHAFIDSILDGKFAKPFGKNEKYSFSSDFDFIGIN
                     YYTKRFIHINKKNIETKTKEGMKTDIGWHYYPQGLYEVVDRFSKRYHKPLMITENGIA
                     DSEDRYRTAYIVSALYSINQAMKEGANVMGYMYWSLMDNFEWVEGNSMRFGLLQTDYN
                     NNVLYMRNSAKIYSKIASDNKIEESTLKFIK"
     gene            complement(5338..>5930)
                     /locus_tag="PLU28_02645"
     CDS             complement(5338..>5930)
                     /locus_tag="PLU28_02645"
                     /inference="COORDINATES: protein motif:HMM:NF020260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG33996.1"
                     /translation="SKTPEYEPEYSPRIIGFLSERYIISEENGKMILTDFHAAHERIL
                     YEEFKKDFFEKGSISCQYLILPEQIELDKTSLEYLAENAAKIKNFGIDLDIHEDGKLL
                     VKALPHSLEKRDIQGLLGEVVAILRLHGIEPIEKIFEDILATMACRAAVKTNDDFVGA
                     QTLLEQVHKRGLKTCPHGRPISMELEVKKIDSYFGR"
     assembly_gap    5931..6030
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6031..6950)
                     /gene="mutL"
                     /locus_tag="PLU28_02650"
     CDS             complement(<6031..6950)
                     /gene="mutL"
                     /locus_tag="PLU28_02650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOG33997.1"
                     /translation="MKSIIHELAQEVVVKIAAGEVITGTFAVMKELLENSADAHADDI
                     KAEIKNGGKSLIKLSDNGCGMSLEDIYNSVKPHATSKISSAEDLYKIQTFGFRGEALS
                     SICQVSRMSISSRPLQEEVGHKVIFIAGKVVSESVVNMNPGTVIEIEDIFFNIPARRK
                     FLKSDSAEGRMTTEIIEKFILSNMVSLSYVRDGKSIYEVRKDDDLQTRLRKVFPELKA
                     DDIIFFEEDGQWIKAHGYITKPIIARLNRTGQIFFVNNRYVRSAELFSVFEGAYSGML
                     QTGKHPYCAVFIDVLPQEVDVNVHPQKLEV"
     gene            complement(6947..7669)
                     /gene="rlmB"
                     /locus_tag="PLU28_02655"
     CDS             complement(6947..7669)
                     /gene="rlmB"
                     /locus_tag="PLU28_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HOG33998.1"
                     /translation="MYIYGRNVIKEIISVRYSVKQVYVSNSKNTDKELASIVQKAKSN
                     GYSVSYVSDDNLKNLVNYDKHQGVVIDIGKDYEYYDTDYLEKKKENAFIVMLDQIQDP
                     QNFGAIIRSAVACGADAIVIPKDNSCEVTAAVIKVSAGQIFKIPIIRVVNLSRTVEYL
                     KEKNYWIFAADMNGKNYYDNDFKGNICLILGNEGSGIREKLREKADFIVSIPMYNSVE
                     SLNVSVSAGIIMFEAAKQRHGI"
     gene            complement(7645..8073)
                     /locus_tag="PLU28_02660"
     CDS             complement(7645..8073)
                     /locus_tag="PLU28_02660"
                     /inference="COORDINATES: protein motif:HMM:NF012843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III domain-containing protein"
                     /protein_id="HOG33999.1"
                     /translation="MIHTLPSLFGHFPISGFSEIPVDSLAYIGDAVIALRYKLYILKD
                     GRIKTNTLNNASKKYLTAQAHSFFIDRLNEKKIFDEIEEGIFKRGYNSKGAKKRGNDE
                     EYRKSTGLEAVIGYLYLSDNYKRLEEIFEVIETCTYMGEM"
     gene            complement(8070..8735)
                     /locus_tag="PLU28_02665"
     CDS             complement(8070..8735)
                     /locus_tag="PLU28_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34000.1"
                     /translation="MKKSLISILVFLFLQMSLFAEGLDGIQYRDDTDNKISIYTLKIQ
                     NADQNLQIHYFSDYEYYTVTNAQSETVSMNFRNSIYDSEYNAVRKSDIISISWKINGK
                     IADKEIKIDSGSWYQAAEYSLMNFVLSGEKSCKYWLVSPADLKATKFVAYNEGIETIT
                     LNKSKVQAYRVRVTLDNWMSVFWSVNYWFRASDGLYIKFEGARGAPGTPKTLVEIIGE
                     QEI"
     gene            complement(8735..9895)
                     /locus_tag="PLU28_02670"
     CDS             complement(8735..9895)
                     /locus_tag="PLU28_02670"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOG34001.1"
                     /translation="MNIAMFSDTYVPQKNGVATALKLYKESMQKMGHRVYMFVPKLGI
                     DSKRHEDDVFEFPAFKFTAEKDHRIALPISTDIFKANKLDLDVIHSHSPFSMGIAADL
                     VAKALDLPYVGTHHTMYEYYRHYVPFIFRPSLKQAQKLIRNWCIKLDKVIAPTKDIKD
                     VLVQYGVPAEHIAVIPTGIDTYSFDGPVLWDIRKEYSIGEEEKILLFVGRLAEEKNIV
                     FLIKMFSKLIKQTDQKYKFVIVGDGPEREKLEELSIELDLHDSIIFTGGQPREKVIDA
                     YKQSDLFVFASYTETQGLVVLEAMAACTPVVALGKMGVRDLLSQKDAGGIMIDELNEN
                     EFIDSIIKLTQNKELYEKYSEKACNFVRNNYSINVTGQKIVEVYSQAIRGIN"
     gene            complement(9909..11075)
                     /locus_tag="PLU28_02675"
     CDS             complement(9909..11075)
                     /locus_tag="PLU28_02675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOG34002.1"
                     /translation="MNIGMFSDTYYPQRNGVATSLKLYKTELERMGHNVYLFIPKLSK
                     NVVKEEKTYQFPAIKFAFEKDQRLAIPIKLDVLRENKLSLDIIHTHTPFSVGIKGMFT
                     AHDFNIPCIGTHHTMYEHYRHYLPVFIRPSLKQTQKGIKYWCEKLNKVIAPTENIRKV
                     LESYGVPNEHIVTVPTGIDTYSFRDAVKWDIHKELSIPSENKVLLFVGRLAKEKNIDF
                     LIKVFRKVHSEIYKSTFVIIGGGNETETLRQLAFDLGLEKDVVFTGSMSRDKVIDAYK
                     GSDVFVFASYTETQGLVVLESMAAGTPVVALGKMGVYDILSNSESGGIMLEDLNEDDF
                     TDSILKILKDEKIKDSLEQRCIKFVDDYYSIASTAKQISMVYSELIEKKEKIIG"
     gene            complement(11077..12045)
                     /locus_tag="PLU28_02680"
     CDS             complement(11077..12045)
                     /locus_tag="PLU28_02680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOG34003.1"
                     /translation="MGLLVILLIGKITDTNVLEEIFKISPLMMFFGFLIFCTGYVVDS
                     IRTIIVLKVLGYKINFFDAFYNNVIGCFFSNITPFAAGGQPLQVYHLSSLGVPSEKGT
                     NFVMSRFLESMVTNLVVSFIFYKPVISVLQGKGVTSKLITVGLVLSVSIAVFFFVLFL
                     NSNIIAKFVNFLEKSKILKKRDLSGKIFKWSEELNGSITFLWKEKIYVMVLDFLLGLA
                     TLVIQAGVLFFFIVMLTKVMYTFNTFMNIFGGMTLLNMVVYYIPTPGASGGTELFYHW
                     FVGDIVKDSAMSLQAVFAWRWSTYYLQIFFGMGTIVFYKLLRKRKS"
     gene            complement(12102..13151)
                     /locus_tag="PLU28_02685"
     CDS             complement(12102..13151)
                     /locus_tag="PLU28_02685"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOG34004.1"
                     /translation="MKVNILSSAGKFPGQGVYSAYMDCVYVLKSTGKVEITENVWNSD
                     AVVQHSHTPDPKVFLQTLFNRRKSLNVVTAHVLPDSIRGSIRNSWILAPLFGIWLKWL
                     YSLNDVVLAVSEDVAVELQKKLNIKKEKIYVFPNFVREDLYLIPDEKIKSEKVRLRKE
                     LGFKDEDFIIIGAGQVQPRKGIKDFVEVAEKLPQFKFVWVGGNPFKDYTEGYHEMKKI
                     TENHPENVFFTGTVSREKVRDYYMLSDIMFLPSYQETFGLVIVEAAGSGLPLLLRDIQ
                     VYTTIFDDCYLKASDNEKFVDYINRLSKDKDLYCEMSCKARKLFEKYSSSRAGINLLN
                     IYSKYYYKKFGKKLF"
     gene            13332..14564
                     /locus_tag="PLU28_02690"
     CDS             13332..14564
                     /locus_tag="PLU28_02690"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HOG34005.1"
                     /translation="MKKIAVIGAQWGDEGKGKIVNYFSKNYDWVARFSGGSNAGHTIF
                     FKDKKYVNHLLPSISLETQSKGYLGSGMVIDLVQMGKELSTLEKDFQGISARFYLDLQ
                     ASLVLPWNKQEDEFFESQRSEPVGTTKRGIGPAYTDKTSREGLRVYDLLDINRLKIKL
                     SELYSMKKKLYGQAITSSDMQVYDELMEGFNFIKSLKVNLMTSIELYRMLDTQSILFE
                     GAQGVMLDIDCGTYPFVTSSTCTAHGVNSFGLSVTELDEVYAVVKAYTTRVGEGPFPT
                     EDSSDKGTILREKGLEFGATTGRPRRTGWLDLPSLRYAILKSKITKLVLTKADVLNGF
                     DEVKICTHYEIDNKNQDIPYSTKDFSIAKPVYVNLKGWHDLKDKTFEEYLLFIEKNLG
                     IKVEFVSYGPKTDEIFKR"
     gene            14589..14969
                     /locus_tag="PLU28_02695"
     CDS             14589..14969
                     /locus_tag="PLU28_02695"
                     /inference="COORDINATES: protein motif:HMM:NF025015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HOG34006.1"
                     /translation="MLIISLIFTVLISLTMIGFGYMLSKKTPKGINSLIGYRSPMSTK
                     NLDTWKFANEYSGRFWIKSGFITAAVSIILAFALQPISIYQKLMFAMIYVQIAVLLLV
                     IPFTEIALRKKFYKTGEKKSDNDY"
BASE COUNT         4774 a         2844 c         2501 g         4799 t
ORIGIN      
        1 ccgtcagaaa aaaaacgtca aaccccatca ttcttttgta cctggcaatt atatccgcca
       61 ctattgtagt ataggaagat ccaatatgcg gttcagaatt tacataataa atcggagtgg
      121 taacatagaa tttcttaacc actgtgattc acctccataa actttttaag ctataattct
      181 ttaaaacaat tataccataa agtgtatacc cttatggtat aattgtaaaa ataaatgtta
      241 agggagtgag caatttgaga ttttccaaac tatatgcacc tacacttaaa gaggctcctg
      301 cagatgcgga tataaaaagc ctcgaacttc ttatacgggg aggttttata cgtaaggtag
      361 ccgccggggt gtatacttat ttacccttgg gttttagagt aattaagaag attgaggaga
      421 tcgtcagaca ggaaatggaa agtatacagt gccaggaaac tatgatgcct ataatacagc
      481 ctgctgaact ttggaaaact acgggcagat gggacgatta cggacctgaa atgatgaaac
      541 ttaatgacag gcataacaga gactttacct taggtccaac ccacgaagag atgataacct
      601 atatagtaaa aaacgaactg cgttcataca aacagcttcc aatcagtctt tatcaaattg
      661 ccaataaata cagggacgaa ataagaccga gattcggagt gctcagagca agagagttta
      721 tcatgaaaga tgcttactcc tttcatactt ctcaggagtc tcttgatgag tcatataaag
      781 atttttattc tgcttacgaa agaatagtaa aaagacttgg agctgcttac tccatagttg
      841 aagccgattc tggtgcaatt ggaggcagcg gctcacatga atttcaggtt cttgccaaga
      901 ctggtgaaag cactattttt tattgcgacg agtgtggata ctccgcaacc gatgaaaaag
      961 ctactgcaat tacagaattt tcttcacaaa acgaagaaaa gaaaagcatc gagcttaagg
     1021 ataccccaaa tgttaagacc attgagcagg ttgctcattt tcttaacgtc ccgcttgaaa
     1081 aaattgtaaa atcgcttttg cttaaaggcc gaaatggctg ggttcttgct cttataagag
     1141 gggattatga aatcaatctc tcgaaagtac gagccgtttt tcaggatcag actatagata
     1201 tgggtgatcc tatggatatc aaaaatactt ttggcgtgga aataggtttt atcggacctg
     1261 ttggcttaaa aggcccacag ataatagcag atgattcggt taaacctctc atgaattttg
     1321 tagtcggagg aatggaaaaa gataagcact ttatcaacgc taatatcgga agagacttca
     1381 ctgtcggcaa gtatagcgat ctacgtgtag ttaaagccgg agaaaattgt cctcactgta
     1441 aaaaacctct caaacaggca cgcggtattg aagtgggtca ggttttcaaa ctgggaacaa
     1501 agtattcctc aagcatgcaa gcctattaca ctgatgaaac aggaaatccc catccctaca
     1561 taatgggatg ttatggttgg ggaatttcaa gaactatggg tgctgttgta gaacagctgc
     1621 atgatgaaaa cggtatttta tggcctgtaa gtatagcacc gtataagata gctgttgttc
     1681 ctgtatcaat gagcgataca gccatggtac agacagcaga aaaaatttac agtgcactgc
     1741 ttgaaaaaaa catagatgtc atactagacg atagaacaga atctccaggt ttcaaattta
     1801 aagatattga ccttatagga attcctctta agattatagc agggaaagga gtaaaagaag
     1861 gaaaagtaga aattaaacta agaaatgaaa agcaaggttt tatgcttagc atagacgata
     1921 ttgataaaat tgtagacttt gccattgaga aacttaatgg ctataatcct acgagtattt
     1981 tagactgaaa gatttcctga gtcttgacaa taactacctt tgatgttata atattcaagt
     2041 gttgaacaga cggagagatg gccgagaggt cgaaggcgct tgcctgctaa gcaagtgtag
     2101 gagtttccta ccgagggtcc gaatccctct ctctccgcca ttttttatat gtccgtgcct
     2161 gtggctcaat tggatagagc attggactgc ggatccaaag attgccggtt caattccggc
     2221 caggcacgcc agtttctgtt taaatatagt tttctgtcta ctgttatgtg aaaattaatg
     2281 tattttttaa gttttggtta caattgtgaa aaaagtaact ttaatttgga ggtgtttatg
     2341 gcaggcgaaa cttacatcga caatgaaaaa agatttttca tcctcaaaac atacgccttt
     2401 tcatttatta tcttcggtct cttattttcc aactatgaga cattttttga tgatatcttc
     2461 agaataatta cagaacctga tttccttata gtagattaca taaaactgtc aggtattggt
     2521 gctgcttttt tgaactgtgg aatagtcacc atggctgcaa taatgattct taaaaaagct
     2581 aaagtcgaga taacaggagt ttctattgcc agtttatgga ccttaagcgg cttttcatta
     2641 tttggaaaaa atattttcaa tgtatggttt gtaataatag gagtttttat atattcaaaa
     2701 attagaaaag aatcattcag aaaatatgtt tatattgcat tttttggtac aagtctttct
     2761 ccgataataa cccagataat ttttagtgat gcacttccat cctttttaaa tcttattctt
     2821 gctttcatca catctacctt tctaggtttt ttgttgcctg cgctttcttc gtactttctt
     2881 tccttccatc gtggatataa tctatacaac gtcggattca ccgctggcat aatagcaacc
     2941 tttttagtat ctattctacg tgcgtacgga tattctttcg aaccaagaat actctggtcc
     3001 agcggaaata atgttctatt aggaatctat ctttatgtgt tgtttttagt aatgatatac
     3061 atgggttaca gatacaataa aaattctttc tccggagtaa aaaaaatatc aacttattcc
     3121 ggcagacttg taactgactt tgttctactt gaaggaatgg aaaaaacact tataaatatg
     3181 ggatctgttg gattagctgc tacttcatac gttctcctta cgggtgccgc cctaaatggt
     3241 ccgactatag gaggaatatt tacaatagta ggttttggtg cttttggaaa acatctgcgc
     3301 aatataattc ccatattttt aggcgtcttt cttggaagta tcaccaatat gtatgatatt
     3361 catgatccca atatggtact cgcagctttg tttggaacta ctctggcccc tatcgcaggc
     3421 gaattcggca tcttttgggg aattatagca ggttttcttc attcatcact ggttacaaat
     3481 ctcggatacc tacatggagg aatgaactta tataataacg gtttttccgg cggattagtt
     3541 gctgcctttt tgcttcctat aatacaagct tttgaaagga ggggaaaaga tgaacactga
     3601 gcgtattaaa gtactgcgaa tagccaacga actcctcaat tacatgtttt taagatgctc
     3661 ccatgctctt acccttgaat taaaagagaa tattttagga ttcgaaatta ctataagatc
     3721 tgcatgctca catattaact ccgaaaatat tgcagaatta aaaaaacttc ttacaccagg
     3781 aaggcacgaa gaaataagcg actactactg gaatcttgca ggaaccaaca gtgaagaaaa
     3841 tgaacttaac cttataggtt tgatgataga ctctgctgag gtaaaagcag aaaacaatga
     3901 acttgtcata aaacttgtac ggaaaaaata aaaaattgtt cttatacttt tgattattaa
     3961 atttttgata aatgtagtat aataaaatta tattggggag tttcatagag aattaggggg
     4021 gattggaatg tccgctacag tacacttccc agaagggttt ttatggggag ctgcaacagc
     4081 atctcatcaa atagaagggg ctaattacaa cagtgattgg tatgaatggg aaaaaaaagg
     4141 aaagataaaa gataactctt cttctgatcc gggctgtcag tcggagaagt attatgaaag
     4201 agatataact tttctttccg atctgaacct caacgcctac agattttcag ttgaatgggc
     4261 aaaaatacag cctgacgaga attctttttg cgagaatcat ttacagagat actgtaaaat
     4321 agttgatata cttctttcaa aaagaataga acctgtggta actttgcacc attttgtgaa
     4381 ccccttgtgg ttttataaca aaggtgcatt tgaaaaacct gaaaacataa gttttttcct
     4441 aaaatttgtt gaaaaaatgg tttctgcttt taaagaaaag gtacagtatt atactgttat
     4501 aaacgaacct atagtatatg ctttcacctc ttatattgat ggttcttggc ccccagggaa
     4561 aaaagactgg aatgccggga tggcagttgt cagaaatctt cttgtggctt atgatgaagc
     4621 ttacaaaata attaaaaaaa taaatcctaa tgccaaaata tctgttgcca agcatactgc
     4681 tgtttaccgc ccatatcttt ctttttcccc tattgatatt atagctagta ggactatcca
     4741 gagatacttt gaccatgctt tcattgattc tatacttgat ggcaagtttg caaaaccttt
     4801 tggtaaaaac gaaaagtact ccttttcttc tgattttgat tttataggca taaattacta
     4861 tactaaaaga tttatacata ttaataagaa aaatattgaa actaaaacta aagaaggaat
     4921 gaaaacagat atcggctggc actactatcc ccagggtctt tatgaagtag ttgacagatt
     4981 cagtaagcgt tatcataagc cgctcatgat aactgaaaac ggaatagctg attcagagga
     5041 caggtacagg acagcatata ttgtcagtgc cctttattcg ataaaccagg ccatgaaaga
     5101 aggggcaaac gtgatggggt atatgtattg gtcacttatg gataattttg aatgggttga
     5161 gggaaacagc atgcggtttg gtctgctgca aacagattat aacaacaatg tgctctatat
     5221 gagaaactct gccaagatat attcaaaaat agcttctgat aataaaattg aagaaagcac
     5281 tcttaagttt ataaaataac gcgtacctca taagaggtgc gctttatttt aagaaagtta
     5341 tcttccaaaa taggaatcga tcttttttac ttccagttcc atgctaattg gtcttccatg
     5401 aggacatgtc ttaagccctc ttttatgtac ctgctccaaa agagtctgtg ccccaacgaa
     5461 atcatcgttc gtctttaccg cagccctaca agccattgtc gccagaatat cttcaaatat
     5521 cttctctata ggttcaattc catgaaggcg caaaatagcc accacttctc ccaaaagtcc
     5581 ctgaatatct cttttttcca acgaatgcgg aagagctttt actaaaagct ttccatcttc
     5641 atgtatatcc aagtcaatac caaaattctt tatttttgct gcattttctg caagatactc
     5701 cagggaagtt ttgtcaagtt ctatctgttc gggaagtata agatactgac aggaaatgct
     5761 tcccttctca aaaaaatctt ttttaaattc ttcgtataaa attctttcat gtgcggcatg
     5821 aaaatctgta agaatcattt ttccattttc ttcgcttatt atgtatcttt ctgaaaggaa
     5881 tccaattatt ctgggcgaat attctggttc atactctgga gtttttgaag nnnnnnnnnn
     5941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tttacttcta gtttctgcgg atgaacattt
     6061 acatctactt cctgaggaag tacgtcaata aaaacagcac aatacggatg ttttcctgtt
     6121 tgaagcatac ccgaataagc tccttcaaaa acagagaata gttcggcaga tctcacatat
     6181 ctgttattta caaaaaaaat ctgtcctgtt ctattaagcc ttgctattat tggtttggta
     6241 atgtagccat gagccttgat ccactgtccg tcttcttcaa aaaaaataat atcatcagct
     6301 ttcagctctg gaaaaacctt tctaagtctt gtttgaaggt catcatcttt tcttacctcg
     6361 taaatactct ttccatcacg cacatacgaa agagaaacca tatttgaaag aataaatttt
     6421 tcaattattt ctgtagtcat acgcccttct gcagaatcac tctttaaaaa tttccttctg
     6481 gcaggaatat taaaaaatat atcctcaatc tcaataacag ttcctggatt catatttaca
     6541 acagattcag aaacaacttt tccagcaata aagattactt tatggcctac ttcttcttgc
     6601 aaaggtcttg aacttatact cattcttgaa acctgacata ttgaagaaag tgcctctcct
     6661 ctgaaaccga atgtctgtat tttatagaga tcctccgctg aagaaatctt gcttgtagcg
     6721 tgaggcttta cagagttata aatatcctca agactcatcc cacagccatt atcagaaagc
     6781 tttataaggg atttgccgcc gttctttatc tcagctttta tgtcatcggc atgagcatct
     6841 gcagaatttt caagcagttc tttcataact gcaaaagtac cagttatcac ttcgccggca
     6901 gcaatcttaa caactacttc ctgtgcaagc tcgtgtatta tacttttcat atgccatgac
     6961 gctgctttgc agcctcaaac attattatcc cggcacttac cgatacattt aacgattcta
     7021 cactgttata cataggaata gatacaataa aatcagcttt ttcccttagt ttttctctta
     7081 taccagaacc ttcatttcct aagatgagac atatatttcc tttaaaatcg ttatcatagt
     7141 agttttttcc gttcatatca gcagcaaata tccaataatt tttttctttc aggtactcta
     7201 cagtacggga aagattaacc actcttatga ttggaatttt aaatatctgt cctgcagaaa
     7261 cttttataac ggcagcagtt acttcacatg agttatcttt gggaataaca atagcatcgg
     7321 caccacatgc cacagctgat ctgattattg ccccaaagtt ttgcggatcc tgtatctgat
     7381 caagcatcac gataaaagca ttttcctttt ttttctcaag ataatctgtg tcataatatt
     7441 cataatcttt tccaatatct attacaactc cctgatgctt atcataattt acaagatttt
     7501 taagattatc atctgataca tacgaaacag aataaccatt cgactttgct ttctggacaa
     7561 tactggcaag ttctttatct gtatttttgg agttgcttac atatacctgt ttaacagaat
     7621 atctcacaga aattatttct tttattacat ttctcccata tatgtacatg tctctatcac
     7681 ctcaaaaatt tcttcaagtc tcttgtaatt atcgcttagg tacagatatc cgataacggc
     7741 ctcaagacct gtagattttc tgtactcttc atcatttcct cttttcttgg cacctttgga
     7801 attatagccc cgcttaaaaa tgccttcttc tatttcatca aaaatttttt tttcattaag
     7861 tctgtctata aaaaatgaat gcgcctgtgc agtcagatat tttttggaag cattattcag
     7921 tgtattagtt ttgatccttc cgtcctttag tatataaagc ttgtatctca aagctataac
     7981 agcatctcct atatacgcaa gactgtctac aggtatctct gagaaacctg atatcggaaa
     8041 atgaccgaat aatgacggaa gagtatgaat catatttcct gttctccaat tatttcaact
     8101 aatgttttag gagttccagg ggcacctcta gcaccttcaa attttatata aaggccatcg
     8161 gaagctctaa accaataatt tacactccaa aaaaccgaca tccaattgtc cagagtaact
     8221 cttactctat atgcctgaac ttttgattta tttagtgtaa tagtttcaat cccttcatta
     8281 tatgctacaa acttggtagc tttaagatcg gccggagaaa caagccagta cttacaactc
     8341 ttttctccag ataacacaaa attcataagc gaatactcgg cagcctgata ccatgagcca
     8401 ctgtctattt ttatctcttt atctgctatt ttaccgttta tcttccaaga aatacttatt
     8461 atatctgatt tccttacggc attatactca gaatcatata tactgtttct gaaattcata
     8521 cttactgttt cagattgtgc gttagtaacc gtataatact cgtaatctga aaaataatga
     8581 atctgaagat tctgatcagc attctggatc tttaaggtat atatagaaat cttattgtca
     8641 gtatcatccc gatactgtat accgtccaga ccttctgcaa ataaagacat ttgcaaaaaa
     8701 agaaaaacaa gtatgcttat taaacttttc ttcatcagtt gatccctcta atagcctggc
     8761 tgtaaacctc tactatcttc tgaccagtaa cgttaattga gtaattgttt ctgacaaaat
     8821 tgcaggcctt ttcagaatac ttctcatata attctttgtt ttgcgtaagc ttaataatac
     8881 tatcaatgaa ctcattctca ttgagttcat caatcattat cccgcctgca tctttctgcg
     8941 aaagaagatc tcttactccc attttcccaa gcgctacaac cggagtacaa gcagccatag
     9001 cttccagaac aaccaatccc tgtgtttcag tataagatgc aaaaacaaag agatcagatt
     9061 gcttataagc atctataacc ttttccctag gttgtccacc ggtaaatatt atactatcat
     9121 gaagatcgag ctctatggaa agttcttcga gtttctccct ttccggtcca tcgccaacga
     9181 tgacgaactt atacttctga tcagtttgtt ttataagctt gctgaacatt tttataagaa
     9241 agacaatatt tttttcttcg gcaagtcttc ctacaaagag gagaattttt tcttcctccc
     9301 caattgaata ctcttttcgt atatcccata aaaccggtcc gtcaaaagaa taggtatcaa
     9361 taccggtcgg aataaccgct atatgttctg cgggaacgcc gtactgcacc agaacatctt
     9421 ttatatcctt tgttggtgca ataaccttat caagtttgat acaccaattt cttataagct
     9481 tttgagcctg cttaagagaa ggtctgaata taaaaggaac ataatgccta tagtattcat
     9541 acatggtgtg atgagttcca acataaggca aatcaagagc tttggccaca agatcggctg
     9601 ctattcccat tgaaaaaggg ctgtgtgaat gaataacatc caaatctagc ttgttggctt
     9661 taaatatgtc agtagaaatg ggaagagcta tgcgatgatc cttttcagcg gtaaatttaa
     9721 atgctggaaa ctcaaaaaca tcatcttcat gtctctttga atcaatacca agttttggaa
     9781 caaacatata tactctgtgt cccatcttct gcatagattc cttataaagt ttaagagcag
     9841 ttgcgacacc gtttttctga ggaacatatg tatctgaaaa catcgctata ttcatgattt
     9901 tctccttatc agccgattat tttttctttt ttctcaatta attcggaata aaccattgat
     9961 atctgttttg ctgttgaagc aattgagtaa taatcatcca caaatttaat acatctttgc
    10021 tctaatgaat cctttatttt ttcatctttt agtatcttaa gaatactgtc tgtaaaatca
    10081 tcttcattta gatcttccaa catgataccg ccgctttcac tgtttgaaag tatatcgtaa
    10141 acccccatct ttcctagcgc aactactggc gtaccggcag ccatggattc aagaaccaca
    10201 agaccctgtg tctcggtata agatgcaaaa acaaagacat cagagccttt gtatgcgtca
    10261 ataaccttgt ctcttgacat agaaccagta aatactacat ccttttccaa tcctaaatca
    10321 aaagccagct gtctgagagt ttcagtttca ttccctccgc cgattatgac aaaggttgac
    10381 ttataaatct cagaatgaac ttttcgaaat actttaatta aaaaatctat gtttttttct
    10441 tttgcaagtc ttcctacaaa gagcaacact ttattctctg aaggtatgct tagctctttg
    10501 tgtatatccc attttacagc atcacgaaaa gaataagtat ctattccggt tggaacagta
    10561 actatgtgct catttggaac gccatagctt tcaagcactt ttcttatatt ttccgtaggg
    10621 gcaatgacct tattcaactt ttcacaccag tattttatac ccttttgagt ctgttttaga
    10681 cttggacgta taaatactgg aagatagtgc ctgtaatgtt catacatagt atgatgtgta
    10741 cctatacagg gaatattgaa atcatgagct gtaaacatac cctttatccc tacagaaaaa
    10801 ggagtatggg tatgaattat gtccaggctt aacttatttt ccctgagaac atcaagcttt
    10861 ataggaattg ctaatctctg atctttttca aacgcaaatt ttattgccgg aaactgataa
    10921 gttttttctt cctttactac atttttggat agtttaggaa taaaaagata gacattatgg
    10981 cccatacgtt caagctctgt tttataaagt tttaatgaag tcgcaacccc atttctttgc
    11041 ggataatatg tatctgaaaa catacctata ttcattttat gatttccttt ttctgagtag
    11101 tttgtaaaac actattgttc ccataccgaa aaatatttgc agatagtatg tactccacct
    11161 ccatgcaaat actgcctgaa gactcatggc tgaatctttc acgatatccc caacaaacca
    11221 gtgatagaaa agctcggtgc ctccgcttgc gcctggagtg ggaatataat atacaaccat
    11281 atttaaaaga gtcatcccgc caaatatatt cataaatgta ttaaaagtat acattacctt
    11341 agtaagcatc actatgaaaa aaaacaatac tcctgcctgg attacaagtg tagcaagccc
    11401 aagcagaaaa tccagaacca tgacataaat tttttccttc cataagaacg tgatactccc
    11461 gttaagctct tcgctccatt tgaatatttt tccactgaga tcccttttct taagaatttt
    11521 tgatttttca agaaagttta caaactttgc aatgatattc gaattaagga aaagcacaaa
    11581 gaaaaacaca gcaatactaa ccgaaagcac caatcctact gttataagct ttgaagtaac
    11641 acctttaccc tgtaaaacag aaataacagg cttataaaaa ataaaggata ctacaagatt
    11701 agtcaccata ctttccaaaa atcttgacat aacaaaattt gtacctttct cagaaggaac
    11761 ccccagggaa gaaagatgat aaacctgaag cggttgtcct ccggcggcga aaggagttat
    11821 atttgagaaa aaacacccta tgacattatt ataaaaagca tcaaaaaaat ttattttgta
    11881 tcctaaaacc ttcaatacaa ttatagttct tatactgtct accacatacc ctgtacagaa
    11941 tatcaggaat ccgaaaaaca tcataagcgg acttatttta aaaatttctt caagaacatt
    12001 tgtatcagta atcttgccta taagcaatat caccagaaga cccattataa cagcaaaaat
    12061 gaaattaaaa atattttttt tgtttttaat ttttactcac ctcaaaacag tttctttcca
    12121 aattttttat agtagtactt agagtatata ttcaaaagat ttatacctgc ccttgaactg
    12181 ctatactttt caaataattt cctggctttg caagacattt cgcaatacaa atctttatcc
    12241 ttgctcagcc tgttgatata atctacaaac ttctcattgt cagatgcctt aagataacaa
    12301 tcatcaaata ttgtagtata cacttgaata tctctaagta acaaaggcag tccgctccct
    12361 gcagcttcaa caattaccag accaaaagtc tcctgataag aaggcaaaaa cattatatct
    12421 gacaacatat agtaatctcg taccttttcc ctggaaactg tacccgtaaa aaaaacattt
    12481 tcaggatgat tttcggttat ctttttcatc tcatgataac cctctgtata atctttaaaa
    12541 ggatttcctc caacccatac aaatttaaac tgtggaagtt tttcagctac ctctacaaaa
    12601 tcttttattc cttttcgcgg ctgaacctgt cctgcaccga ttattataaa atcttcatct
    12661 ttaaagccca actcctttct cagtcttact ttttcacttt taattttttc gtcgggaatt
    12721 agatacaaat cttcccggac aaaatttgga aaaacataaa ttttctcttt ttttatatta
    12781 agttttttct gaagctcaac tgcaacatct tcactgacag caagtacaac atcattaaga
    12841 ctataaagcc atttaagcca tattccaaat agaggtgcca gaatccatga atttcttata
    12901 cttcccctta tagaatcagg aagcacatgc gctgttacaa catttaaaga tttcctcctg
    12961 ttgaaaagtg tctgtagaaa aaccttaggg tcaggagtat gcgaatgctg aaccaccgca
    13021 tcactgttcc aaacattctc agtaatttca accttacctg tagatttgag tacataaaca
    13081 cagtccatgt acgccgaata aacgccctgc ccgggaaact tccccgcaga ggaaagaata
    13141 tttactttca tatttttcct acctttcaca cttttaaaat tttcatatat attatatcat
    13201 ttatatctaa aaaaattatt gttcttgtgt tatactatat tataacacta taagttgtct
    13261 ttgtcaattt cactttatag aggttttgat gtataatatt attgttcatg aaattcatca
    13321 gaggtgagaa aatgaagaaa attgcagtca taggagccca gtggggcgat gagggaaaag
    13381 gaaagatagt taattacttt tcgaagaatt atgattgggt tgcacggttt tcaggaggtt
    13441 ctaatgccgg gcatacaatc ttttttaaag ataaaaaata cgttaatcat cttcttccat
    13501 ctatttcttt ggagacacag tccaaaggat accttgggag tggtatggtc attgacttag
    13561 tacagatggg aaaagaactg tcaactctgg aaaaggattt tcagggtatt tctgcacgat
    13621 tttaccttga tctgcaagca tctttagttt taccttggaa caaacaggaa gatgaattct
    13681 ttgaaagtca acgctcagaa cctgttggta caacaaaacg tggaattggt cctgcttata
    13741 ctgataaaac atccagagaa ggattaagag tctacgatct tctagatata aacagactta
    13801 aaataaagct ttcggaacta tattcgatga aaaaaaagct ttacggtcaa gcaatcactt
    13861 ccagcgatat gcaggtttat gacgaactca tggaaggatt taattttata aagtctttaa
    13921 aagtaaatct gatgaccagt attgaactct accgtatgct tgatacgcag tcaatacttt
    13981 ttgaaggtgc acagggggtc atgcttgata ttgactgcgg cacttatccg tttgtcactt
    14041 caagcacatg tactgctcac ggagtaaact cttttggact gagcgtaaca gaacttgatg
    14101 aggtctatgc cgttgtaaag gcttatacaa ccagagtagg cgaaggacct tttcctacag
    14161 aggatagttc tgacaaagga accatcttac gtgaaaaagg actagagttc ggcgctacca
    14221 caggacggcc cagaagaaca ggatggcttg atcttccatc tcttcgttat gctatactaa
    14281 aatctaaaat aaccaagctt gtactaacaa aagcagatgt tctaaatggc tttgacgagg
    14341 ttaagatctg cacccactat gaaatcgata ataaaaacca agatattccc tactctacaa
    14401 aagatttttc tattgccaaa cctgtatatg ttaatttaaa aggctggcac gacctgaaag
    14461 ataaaacttt cgaagagtat ctcttattca tagaaaaaaa tctgggaatc aaagtagagt
    14521 ttgtctcata tggtccaaaa accgatgaaa tattcaaaag ataatattat ttatgtgtgg
    14581 aggataaaat gcttattata tccctcatat ttactgtact gatttcgctg acaatgattg
    14641 gattcggata tatgctatca aaaaagactc cgaaaggaat caattctctc atcggttaca
    14701 ggtctccaat gtcgactaaa aacctggata cttggaagtt tgctaatgag tactcaggaa
    14761 ggttttggat aaaaagtgga tttataacag ctgctgtatc aataattctt gcttttgcgc
    14821 ttcagcccat aagtatttac cagaagctta tgtttgcaat gatatatgtc caaatagccg
    14881 tactgctttt ggttatacct tttacagaaa ttgctcttag aaagaaattt tataaaactg
    14941 gcgaaaaaaa atctgacaat gattactgaa gtaaatattt ttcaacctgg aataataaaa
    15001 aatccaggtt gatttttt
//
LOCUS       DAOSHR010000028        14776 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_3333_length_14776_cov_6.021670, whole genome shotgun sequence.
ACCESSION   DAOSHR010000028 DAOSHR010000000
VERSION     DAOSHR010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 14776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14776
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3333_length_14776_cov_6.021670"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="PLU28_02700"
     CDS             complement(<1..288)
                     /locus_tag="PLU28_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34007.1"
                     /translation="MSQICHLPYGMDDPYLNNLSLQRIPKDPLADEEVLINFQTFPMQ
                     AGQRAWIISSVNGMPHVKTRARFDYAQNGWALWTCCLGRFRKGDKVRYRIFS"
     gene            complement(303..1526)
                     /locus_tag="PLU28_02705"
     CDS             complement(303..1526)
                     /locus_tag="PLU28_02705"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HOG34008.1"
                     /translation="MKKLLLAVLSVCLFVVAFGAVEITYMNYSALPSHEETLKLIVKE
                     FEKQNPGIKIKLVNVPWDDYFLKLSTSFAGNDAPDTFEINYENFVEYASDGLLLPLDS
                     LNKQFNFNANEIFAEKAYKAFSHNAVQYGFPEKFSTVVLFYNKKLFDEKKLAYPTNNW
                     TWKEEMEAAKKLTDSKKGIWGVYQPVQFWEFYKVLTQAGGSFITANKASLNSKAGIDA
                     VNWLVDKQKLGVMPTDAQAGGLKSEDLFVAGKVAMVHTGIWMFKMFRDEASFDWDIVV
                     EPGMESKGTHYFVDGVVASKNTKHKEEAWKWIKFLTTSEYNAKLRYEKGWELPAISNE
                     KYLEPFLTEKSKPANKKAIFESLSYSSLPPVIKGWSALSDIVNKELSDVALGNKTAQD
                     ALKAIDIEINKKLGK"
     gene            complement(1557..2378)
                     /locus_tag="PLU28_02710"
     CDS             complement(1557..2378)
                     /locus_tag="PLU28_02710"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOG34009.1"
                     /translation="MNINIRKVVVYLLLVMLALTTIVPMLWTFSTSLKSKQATLAFPP
                     DIVPKEPTLNNYITLFNSYPFGRYLFNSFLVTSATVFLQVLIAGLAAFAFARFDFRFK
                     NVLFGLYLATLMIPFQVTAIPLYLIVRNFNWINTYQGLIVPRLFSAFGVFLLRQAFMS
                     TPREFDESAFIDGAGYFTVFFKIVLPMNKTALATFGIFSFMDAWNSYLWPLIVSTEQN
                     MMTLPVGLANLHGRWITQWNLVSAGAMISIIPILLVFLFAQKWFVQGVASSGLKG"
     gene            complement(2375..3244)
                     /locus_tag="PLU28_02715"
     CDS             complement(2375..3244)
                     /locus_tag="PLU28_02715"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOG34010.1"
                     /translation="MKKKDRKYIFWFLAPSLAVFLIFQFIPMIWSFIISFTDWNVRTL
                     PKWVGLGNYIEMFSDKNFYEAVIHTFQFIIMYIPLVLIGGMMMAMLVNLKIKGIGFFR
                     SFYFIPVVSSWVAVSLIWKGILNPAYGYLNYFLSLFGIEGPQWLYDKNWAMVGVVMAS
                     VWKDIGFTMVLLLGGLKNISEEILEAAQIDGAGRLKKFFKITLPLMSPQIFFVVMISM
                     INSFQVFDQVMVMTNGGPAGATTVLVEQIYRNAFSYSRMGYACAMSWILLLIILVFSF
                     IQNRAEKKLVFYS"
     gene            complement(3254..4441)
                     /locus_tag="PLU28_02720"
     CDS             complement(3254..4441)
                     /locus_tag="PLU28_02720"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOG34011.1"
                     /translation="MQTIGSKELIREINEKLILKEIYLNKKIDRTSLIKNTGLSGGTI
                     TKIISCLIEKQLVKEIGEADSTGGRKPILLVLNKDYARIIGIKIGVGYIDFILTDLTG
                     DVIFSQTIKYEGSISPQKVVSMITEFHAKFHDKHLLGISVAVSGIVNPNTGTVVNSFL
                     LGWKDVELAQMIKKEVDTYIFVVNDVDSFSLSQLYNGNARFYKNSVVMTLGVGIGGSL
                     IIDKKIHTADGGVGEIGHMSVVPNGNKCTCGSRGCLEAESSIAAIAARINKATTVAEL
                     KKIFSSVSNTEISELEFIRKAIDLDNKACKKVFKEFSVEIGIAMKNLINILAPDYFLL
                     GGETLEFRDLFMKDAIEFAKKNAFGTLGTKVCIETDTLGRNAWTLGGIYKIIEVNLFE
                     NRI"
     gene            4704..5594
                     /locus_tag="PLU28_02725"
     CDS             4704..5594
                     /locus_tag="PLU28_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOG34012.1"
                     /translation="MRTELKSMHLSNGETLGYREREGGKEVLLLIHGNMTSSKHWDLL
                     MDAMDPKYKIFAVDMRGFGISSYNKRFDSLMELCEDIKLLVDGLKLKHFSIMGWSTGG
                     GVAMHFAANYPDYIKKVILLESVGTRGYPIFRKDSSGKPIIGEYIKTKQELENDPVQV
                     KPILMAYQTKNKEILKAIWNGVIYNHNQPTSSKYEEYLEDMLTQRNLVDIDYSLQYFN
                     ISNDFNGVINGTGEAAKIKCPVLILWGKNDLVVPEYMALDIKKDIGENAELVYLEGCG
                     HSPLIDNLGLLVRTVQDFLA"
     gene            complement(5632..6378)
                     /locus_tag="PLU28_02730"
     CDS             complement(5632..6378)
                     /locus_tag="PLU28_02730"
                     /inference="COORDINATES: protein motif:HMM:NF018816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOG34013.1"
                     /translation="MKTLVYSGLMVALVFVTTFSIKVPVPFTQGYIHMGDSMIFVASI
                     LFGWKVGALAGGVGSMLADIVGGYAGWAVPTLIIKSVMGIIVALVAAEYKKKKTAKGI
                     ISLLSMGAWVIFGITLNAVLNSVLADSTSAQKLAGQLELSTVQELQTTAGGVSTGILI
                     GISLIPVCVLLLWLFLRRKDSTMVTFSHLLGMTVAGMWMVAGYYFAERIMFGNWLIPI
                     FALPMNILQFVGGMIIAFIIVIGLRKRKAL"
     gene            complement(6515..7474)
                     /locus_tag="PLU28_02735"
     CDS             complement(6515..7474)
                     /locus_tag="PLU28_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34014.1"
                     /translation="MRKKVLVSLVLILSILTLSALRSDLLLYRNELKTLLIDKYKISG
                     YDLSETIYRERYFRYRLNQSLYIGNWSWIDKSLSLDENFNERYLLETYSLDIKIDPKT
                     IESAIAELQYFAPKSTLVQINNSLYTIDGLDKNRLKTLLISCSFDVSNENSFFSKLDI
                     FNLFSYNTTKKILKSDFESLIFRLVSENTNNYIIVPLKNFYGKIDKELWFKNTMFSFR
                     INRAENYMNPEIRYKLDSYREYFSENRQYKFVDYYKLKDLAESGYPDLALLIMVFNID
                     NVYLRTADKEDLTQYFSDMSSYLKTVKEETAAEKLILMLPVND"
     gene            complement(7488..8411)
                     /locus_tag="PLU28_02740"
     CDS             complement(7488..8411)
                     /locus_tag="PLU28_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34015.1"
                     /translation="MQLSQKHFCDARDFIYKNARELDKAVFRYIFEKGSKLDVFSELE
                     KFQNPDGGFGKGIEPDFWLTDSSPAATAKALEYLYIADADISQGLVVDAIYYLIDSFN
                     YEEGKWRATGSRVNDFPHAPWWEYNFSKKNSEAGTLSGWGIPNPSISGYMNLYKELVP
                     YDFLKMINERAIGFINSIHELNRYDLIEYSKFYDLSPKKIKDRISDKYFYFVGKEIII
                     SPEKWDKYATQPLMFVTDKESELVYILKDSIDKNLDYIISRQKPDGCWEPEWSWGKEN
                     ENWQKAKEIWKGFQTFNNLRLLNMFGKIEEA"
     gene            8572..9018
                     /locus_tag="PLU28_02745"
     CDS             8572..9018
                     /locus_tag="PLU28_02745"
                     /inference="COORDINATES: protein motif:HMM:NF024804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HOG34016.1"
                     /translation="MDYRKIMSRFSAALNQVNAAYAVIAKKYGLSYNALMVMYIIAED
                     ETVTQKNICDILFLSKSTVHGILLELIKNKYVVLISGKNKKEKRIIFTNNGEEFFSAI
                     LKDTEIFEKKVLDFIGEKDTLFFLEQSERAANYMLSKVNETINRDD"
     gene            9020..9631
                     /locus_tag="PLU28_02750"
     CDS             9020..9631
                     /locus_tag="PLU28_02750"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOG34017.1"
                     /translation="MNIEIKELQKKDFKKVIEFAVKGMHFDRYTENKWELDIYGRYFL
                     YLELQRASQVLAAYIEDRLVGVLMADMKGEKKKYLSVWRKVYLFLIEKALSVGYKSGT
                     DKYDEANQEMYSRYIKHNIPDGEICFLAADSTVQGKGIGSCLLKRLEELERGKRIFLY
                     TDSNCTYQFYERKGFKRVEEKEIKLEIHGKEVPLVCYLYDKKL"
     gene            complement(9919..9994)
                     /locus_tag="PLU28_02755"
     tRNA            complement(9919..9994)
                     /locus_tag="PLU28_02755"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9959..9961),aa:Cys,seq:gca)
     gene            complement(10047..11342)
                     /locus_tag="PLU28_02760"
     CDS             complement(10047..11342)
                     /locus_tag="PLU28_02760"
                     /inference="COORDINATES: protein motif:HMM:NF017509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOG34018.1"
                     /translation="MEKQLLAQDMNIKRYLIRFPQEEVLKAENEIVAEVNQHYSFEGF
                     RKGKAPKALIKARMSESFDTWIKDYLVDLAMTDIEKEEKNLFDPMIEASSQNEGVIEI
                     EVKIHAYPKILSTKFSDMQVEVVKKVQVVEKYVEDRLNQLLDQNAILDPKDGPAELED
                     FVRTTYSVINAEGKVITDKKESEYVLYKEDERPQVKEIVGKKAGETVTFERKFEEKSY
                     TYTINILEVFKRTIPEISDDFVQGIGADVKSLVELKEKLTDEGSQAYENWKNDYIKNY
                     IVGELANYVEVELSDETIDFYAQRYIDSLKKENKYEEELGKHENDEAKMLEEVKKSSV
                     KWIKELAVVDSIVKQKDIKAEKEDIDSAITSLAQNVWYMQPERAREMVYSNQKLMNEI
                     VWEVLRGKVAYEISSEVKLIEIDKDHDHEHDHEHEHNHE"
     gene            complement(11440..11925)
                     /locus_tag="PLU28_02765"
     CDS             complement(11440..11925)
                     /locus_tag="PLU28_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOG34019.1"
                     /translation="MWMIISDTHDNMKVVEEVPYVVEKYKVDSIFHCGDFVAPFVLKS
                     FMIPGVDFFGVFGNNDGEKVLLSKKSDGRITYGPTAIEHQGKKIFMMHEPYSLMPAES
                     SGLYDYVFFGHTHEVVFRQHGKTLIINPGEACGWVRGIASCVLLEPDSNKYVLHKFIQ
                     N"
     gene            complement(11937..12584)
                     /locus_tag="PLU28_02770"
     CDS             complement(11937..12584)
                     /locus_tag="PLU28_02770"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HOG34020.1"
                     /translation="MINIVYIVSGGSLLSSDEFYINMIKSASVTIACDSGIKLFRKFN
                     IAPSYLIGDMDSAEENDVEWAKSIGVKILKFPPEKNELDTELALIMARGLQENKAVLS
                     GVLGSRIDQTMAGIYLLAAYNDLDSEINEDYINIGTVKNRRLFTALKGETWTVLPIGG
                     EVKGLNLRGFKYELTNKYLNAWDTLGVSNEAAKEKVEISLNEGIICYIRWIGKKV"
     gene            12712..13578
                     /locus_tag="PLU28_02775"
     CDS             12712..13578
                     /locus_tag="PLU28_02775"
                     /inference="COORDINATES: protein motif:HMM:NF012400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b5 domain-containing protein"
                     /protein_id="HOG34021.1"
                     /translation="MKKHLMILFVVFMTTVGLSEALSDLDLKNYNGDTVLISMTTLKS
                     STDLLAYNGMVYNISAYSDLKNISKEKFFSVGTESLRILKKDAVIEKKLASVKTIGYL
                     AVSVKELETFNGKSSSVPAFFASDGKIYDVTENIKCRGGVHTVAYQPGKDITIDLKKS
                     KHSNNVIKGLLLIGILTFNFEDLYAMNGKNGKPSLVGVNGKIYDFSELKRWAGGEHLN
                     VHSAGQELTYQLKSKSPHGVKKISSASVIGLLATDMKGFSTVISTNKNLKYSTKEQNI
                     YEEKEIIGFLTK"
     gene            complement(13720..>14776)
                     /locus_tag="PLU28_02780"
     CDS             complement(13720..>14776)
                     /locus_tag="PLU28_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34022.1"
                     /translation="FNSLKHFVRANFVLLKIFDAKCDKDSFFRVYKSLKEVISNLSKE
                     LEYDCRMNLAVAEMSLFNDSRRIDRLLPFRDKNRNAVLYYHKAIASIELKNDYEQGLK
                     YIELSKNESQSSDSPFLKLMIMNAYISYNLKYKKEQHIVRLVQKMAFLIGYWFDIQEY
                     MSLPSVFYTVAEISGSTLEKDFFKKIIEELTKKEITYFQNGHRYFISENFVSESKLPL
                     LQSISHIRDLSLQSRIRKNQKIICETQYEKGWLKRAEEYLKLFDLAKREKFIDFYFSS
                     SEKPTINRFITSYPIYKSYNRYIEKIICKKKFHYTFFENTCFDPIVATITCSIWNLKE
                     KKSAVDIIENMINYVFS"
BASE COUNT         4736 a         2769 c         2413 g         4858 t
ORIGIN      
        1 tgaaaatata cgatatctca ctttgtcacc ttttctgaat ctgcctaagc agcatgtcca
       61 aagtgcccag ccgttttggg cataatcaaa tctagccctt gtctttacgt gcggcatacc
      121 gtttaccgaa gatataatcc atgctctctg acctgcctgc atcggaaagg tttgaaaatt
      181 tattaacact tcctcatctg caaggggatc ttttggaatt ctttgaagac ttaaattatt
      241 aaggtacgga tcatccattc cataaggaag gtgacagatc tggctcattt cttcacatcc
      301 ttttattttc cgagtttttt atttatttct atatcaatag ccttaagagc atcctgtgca
      361 gttttatttc cgagtgcaac atctgaaagt tctttattaa ctatatctga taatgcgctc
      421 cagcctttta ttacaggggg aagcgaagaa taactgagag attcaaagat tgctttctta
      481 tttgccggct tagatttttc ggtaaggaaa ggttcaagat atttttcgtt agagattgcc
      541 ggtaattccc atcctttttc atatctcagc ttagcgttat actcgcttgt agtaagaaat
      601 tttatccatt tccatgcttc ttccttatgc tttgtgtttt tacttgctac gacaccatct
      661 acaaaatagt gtgttccctt tgactccata cctggttcta ctactatatc ccagtcaaag
      721 cttgcttcat ccctgaacat tttaaacatc cagattcctg tgtgaaccat agcgactttt
      781 cctgctacaa aaagatcctc tgacttaaga ccgccggcct gggcgtctgt tggcataaca
      841 ccaagtttct gtttgtcaac aagccaattt acggcatcaa ttcctgcttt tgagtttaaa
      901 gatgctttgt ttgctgtaat aaagcttcct cccgcctgtg taagtacctt ataaaattcc
      961 cagaactgaa caggttgata aacgccccat atcccttttt ttgaatcagt aagctttttt
     1021 gcagcttcca tctcttcttt ccatgtccag ttgttggtag gataagctaa ttttttctca
     1081 tcgaaaagtt ttttattata gaaaaggact actgttgaaa acttttcagg aaatccatac
     1141 tgaacagcat tatgtgaaaa agctttatat gccttttcag caaagatctc gttggcattg
     1201 aagttaaact gcttattcaa tgaatccagc ggaaggagaa gtccatccga agcatactca
     1261 acaaagtttt cataatttat ttcaaaggta tcgggagcat cgttaccggc aaaactggta
     1321 gaaagtttta aaaaataatc gtcccaagga acatttacaa gctttatctt aataccggga
     1381 ttctgttttt caaactcttt tactataagc ttcaatgttt cttcatgact tggaagagcc
     1441 gaatagttca tataagttat ttccactgct ccaaatgcaa ctacaaaaag acataccgat
     1501 aataccgcca acaagagttt cttcataata cacctcccca agtgtacttt ttattttcag
     1561 ccttttaagc cgcttgatgc tacgccctga acaaaccact tctgtgcaaa aaggaaaaca
     1621 agcagtatcg ggatgatgct tatcatagct cctgcactta caagattcca ctgagttatc
     1681 catcttccgt gaagattggc cagacctacc ggaagagtca tcatattctg ctcggtagaa
     1741 actataagtg gccataggta actattccat gcatccataa aagaaaaaat cccaaaagtt
     1801 gccaaagcag ttttattcat aggcagtact attttaaaaa atacagtaaa gtaacctgct
     1861 ccgtctataa aagcagactc atcgaactcc cgcggagtag acataaaagc ctgtctcaga
     1921 agaaatactc caaaggcact gaaaagtctt ggtacaataa gtccctgata tgtatttatc
     1981 cagttaaaat ttcttacgat gagatacaaa ggaattgctg ttacctgaaa cggaatcata
     2041 agagttgcaa gataaagacc gaataaaaca tttttaaatc taaaatcgaa ccgtgcaaat
     2101 gcaaatgctg caagaccagc tattagaact tgaaggaaaa ctgttgctga agttaccaaa
     2161 aaactattga acaaatatct cccaaaagga taagaattaa acagcgttat atagttgttt
     2221 aaagtaggct cttttgggac tatatccgga ggaaaagcta aagttgcctg tttggatttc
     2281 aaagaggtag aaaatgtcca aagcataggc acgatagttg tcaaagcaag cataacaagc
     2341 aaaagatata caacaacttt tcgtatattt atattcatga gtaaaacacc agctttttct
     2401 cggctctgtt ctggataaaa ctaaaaacaa gaattataag aagaagaatc cacgacatag
     2461 cacaagcgta tcccatacgt gaataactga aagcatttct gtatatctgt tctacaagaa
     2521 ccgttgttgc tcctgccggt cctccgttag tcataaccat gacctggtca aagacctgaa
     2581 aagaatttat catggaaatc ataactacaa aaaatatctg tggactcatg agtggaagag
     2641 tgattttaaa gaattttttc agcctacctg caccatctat ctgagcggct tccaatattt
     2701 cctcacttat atttttaagc ccaccaagta acagaaccat tgtaaaacct atatctttcc
     2761 atacgcttgc cataacgacc ccgaccatag cccagttttt atcgtataac cactgcgggc
     2821 cttctatgcc aaataaggag agaaaatagt taagataacc gtatgccgga ttaagtattc
     2881 ctttccatat caacgacaca gccacccatg atgatactac gggaataaaa tagaaagatc
     2941 tgaaaaatcc tatacccttg atctttaggt tgaccagcat ggccatcatc attccaccga
     3001 ttaaaaccag agggatatac attatgataa attgaaaggt atgaataacc gcttcataaa
     3061 aatttttatc agaaaacatt tcaatgtaat tacccaaacc aacccatttt ggtaatgttc
     3121 ttacattcca gtcagtaaaa cttattataa aggaccatat catcggaata aactgaaata
     3181 tcaaaaaaac agccaagctc ggagcaagaa accaaaaaat atattttctg tctttttttt
     3241 tcatactctc cccctatata cggttttcaa agaggtttac ctcaattatt ttatatatcc
     3301 ctcccaaagt ccatgcattt ctgcctaaag tatcggtttc tatgcaaact ttagtaccca
     3361 gcgtaccaaa ggcgtttttt ttggcgaact caattgcatc cttcataaat aaatctctga
     3421 actcaagtgt ttctccgcct aacagaaagt agtcaggtgc aagtatattg ataaggtttt
     3481 tcattgctat accaatttct accgaaaact ctttaaatac ctttttgcat gctttattat
     3541 caagatcaat tgcttttctt ataaactcaa gttcggatat ttctgtatta cttacagatg
     3601 aaaaaatctt tttaagctca gcgacagtgg ttgctttgtt tattcttgcc gctatagctg
     3661 ctatcgaaga ctctgcttca agacatcctc tgcttccgca agtacattta tttccgttag
     3721 gaacaacaga catatgtccg atctccccga cacctccatc tgcggtgtga atttttttat
     3781 caataataag agagcctcct attcctactc ctaaagtcat tacaacagag tttttataaa
     3841 atcttgcatt tccattgtaa agctgggaaa gggaaaatga gtctacatca tttacaacaa
     3901 aaatgtaagt atcaacttcc ttttttatca tctgtgcaag ttcgacatct ttccatccta
     3961 ataggaaaga gtttacaacg gtacctgtat taggatttac tattccggat actgcaacag
     4021 agattcctaa aaggtgctta tcatgaaact tagcatgaaa ttctgttatc atagaaacta
     4081 ccttttgcgg acttattgaa ccttcgtatt ttattgtctg agagaaaata acatctcctg
     4141 ttaaatctgt aagaataaaa tcaatataac ctacgcctat ttttataccg attattcttg
     4201 cataatcttt attcaagaca agaagtatgg gttttcttcc tccggttgaa tcagcttcac
     4261 caatttcttt tacaagctgt ttttctatca gacatgaaat aatttttgtt attgttccgc
     4321 cactaagccc tgtgttttta attagagatg tccggtcaat ttttttatta agatagattt
     4381 ctttaagtat aagtttttca tttatttcac gtataagttc cttgcttcct attgtttgca
     4441 tgctttcacc tctacttact ttcttcagtg aagaaagtat cttagtctaa gtatatcatt
     4501 aatgcttttt gaatttcaac agtattatcg gtaattaaga ataaagaata acgataaatg
     4561 agaaatattc cctaaaaaag taaaaattca ttctctaagt acatttttta caattattaa
     4621 tttctaaagt atatttttac ttttttactt aaaatgtgat acaatatcat atgaatcact
     4681 tcttactcaa aggaggaaag catatgagga cagagttaaa aagtatgcat ttatctaacg
     4741 gagaaacttt aggatacagg gaaagagaag gcgggaagga agtgctttta ctcatacacg
     4801 gcaatatgac atcctctaag cattgggatt tgttgatgga tgccatggat ccaaaataca
     4861 aaatttttgc tgttgatatg cgtggattcg gtatttcctc atataataaa agatttgact
     4921 ctctcatgga gctttgtgag gatattaagc ttctggttga cggtcttaag cttaagcact
     4981 tttccataat gggctggtct acgggaggtg gtgtagcgat gcactttgct gcaaactatc
     5041 ctgactatat aaaaaaagtg attctgcttg aatctgttgg aacaagaggt tatccaattt
     5101 ttagaaaaga ttcttcaggt aagccaataa taggagagta tattaaaact aagcaagagc
     5161 ttgaaaatga tcctgtacag gtaaagccta ttctaatggc ttaccagacc aaaaacaaag
     5221 aaatacttaa agctatttgg aacggggtta tttacaatca caatcagcca acttcttcaa
     5281 aatacgaaga gtatcttgaa gatatgctta cacagagaaa ccttgtggat attgattact
     5341 cactccagta cttcaatata agcaatgatt tcaacggtgt aataaacggt acgggagaag
     5401 cagcaaagat caaatgtcct gttcttattc tctggggaaa aaacgatctt gttgttcctg
     5461 agtatatggc gctagatatt aagaaagata ttggagaaaa tgcagagctg gtttatcttg
     5521 aaggatgtgg acattcgccg cttatagaca atctcggttt gcttgtcaga accgttcagg
     5581 acttcctcgc atagaaaaac aggcatgaat atcatgcctg ttttttgatt tttaaagagc
     5641 tttacgtttt cgtaagccta ttactataat aaaagcgatt atcattcctc cgacaaactg
     5701 cagtatgttc ataggaagag caaatattgg aataagccaa ttgccgaaca taattctttc
     5761 cgcaaagtaa taacctgcaa ccatccacat gcctgccaca gtcattccca gaagatgtga
     5821 aaatgtaacc atagtactgt cttttctacg caggaaaagc cagagcaaaa gcacacatac
     5881 gggtattaaa ctaattccga tgagtatacc agtacttact ccaccggcgg ttgtctgtaa
     5941 ctcttggacg gtagacagtt caagctggcc tgcaagcttc tgagcacttg tgctgtcagc
     6001 cagaactgag tttagtacag cattgagagt aattccaaaa ataacccacg ctcccatgct
     6061 taacaaagat attattcctt tggctgtttt tttcttcttg tactctgcag caacaagagc
     6121 aacaataatt cccatcaccg attttataat aagtgtcgga actgcccatc cggcgtagcc
     6181 tccaacaata tcggcgagca ttgaacccac tccccctgca agagctccta ctttccagcc
     6241 gaaaagtata gaagcaacaa atatcataga gtctcccatg tgaatatagc cctgcgtaaa
     6301 aggaacaggc actttaatgg aaaaagtggt cacaaaaacc agagctacca taagtccact
     6361 gtaaactagt gtttttatcc ttgagatagc aatcccccct taatggttta ttcgggatta
     6421 attataatca tcaaacagaa aaatcagtaa gtcacaccgg taaataaaaa gtaaaacata
     6481 aaaaagcttt ttcccagtcc aattttataa agatttaatc atttacaggc agcattaata
     6541 tgagtttttc agctgcagtc tcttctttaa cggttttaag gtaggaggac atatcggaaa
     6601 agtactgtgt tagatcctct ttatcggctg ttcgcagata cacattatca atgttgaaga
     6661 ccataatcaa aagcgcaaga tctggatatc cgctttctgc aagatcttta agcttatagt
     6721 aatcgacgaa cttgtactgt ctgttttcgg aaaagtactc tctgtaagag tctaacttat
     6781 atcgtatctc tggattcata taattttctg ctctgtttat cctgaatgaa aacatagtgt
     6841 ttttgaacca gagttccttg tctatctttc cataaaaatt tttcaaaggt actattatat
     6901 agttattggt attttcagaa actagcctga agataagact ttcaaagtca gattttaaaa
     6961 tctttttagt tgtattataa ctgaaaagat taaagatatc aagtttactg aaaaaagagt
     7021 tttcatttga aacatcaaaa gaacaggaaa tcaacagtgt ctttaacctg tttttatcaa
     7081 gaccgtctat cgtatacaaa gagttgttta tctgaacaag tgttgacttg ggagcaaaat
     7141 actgcagctc agctattgct gattcaatag ttttaggatc tatcttaata tcaagactat
     7201 aggtctccag aagatatctt tcgttaaagt tctcatccag actgagagat ttatctatcc
     7261 aagaccagtt tccaatgtaa agcgactgat taagcctgta tctaaagtat ctttccctat
     7321 agatagtctc tgaaagatca tatcctgaga ttttatattt gtctatcaat aaggttttaa
     7381 gttcattcct atatagaagc agatcactcc tcaaggctga aagcgtcagt attgatagta
     7441 ttaaaaccaa tgataccaaa actttttttc tcattcaact ctctccttta cgcttcttct
     7501 atttttccaa acatattaag aagtcttaag ttgttgaaag tctgaaaacc tttccatatt
     7561 tcttttgcct tttgccagtt ttcattttct tttccccatg accattctgg ctcccagcat
     7621 ccatcaggtt tctgtctcga aatgatataa tcgagatttt tatctattga atccttaaga
     7681 atgtaaacaa gttcagattc tttatcagta acaaacatca atggctgtgt tgcgtactta
     7741 tcccattttt caggacttat gataatctcc tttcccacaa agtaaaagta cttatctgaa
     7801 atacgatcct ttattttttt tggagataga tcataaaact ttgaatattc aataagatca
     7861 tatctgttga gttcgtggat actgtttata aaaccgattg ctctttcgtt tatcatctta
     7921 agaaaatcgt atggcacaag ctctttgtac agattcatat atcccgaaat agaaggattt
     7981 gggatacccc agccacttaa agtacctgct tcagagttct tttttgaaaa attatactcc
     8041 caccatggag catgtggaaa atcattgacc ctgctcccgg ttgccctcca cttcccttct
     8101 tcataattaa aactatcaat aaggtagtat atggcatcga caactaatcc ctgcgatata
     8161 tcggcatctg caatgtaaag atattcaaga gcttttgcag tggcagcagg agaagagtct
     8221 gtaagccaaa aatcaggttc gattcctttt ccgaatccgc catctggatt ttggaatttt
     8281 tccaattcag aaaaaacatc gagttttgag cctttttcaa aaatatatct aaaaactgcc
     8341 ttgtcaagct cacgagcgtt tttatatata aaatcccttg catcgcaaaa atgcttctgc
     8401 gaaagctgca cacatactcc cccttttaat gttcatgttt ataaaattat acatcataaa
     8461 gaatgtaaat aaagaagaca catatgaaaa taaaaatata cagctcactg cttgacaata
     8521 gtgcgttttc gtactataat tatggtacta aaacgtacta tggagggaaa aatggattat
     8581 agaaaaataa tgtctaggtt cagcgctgcc ttaaatcagg taaatgcggc atatgcagta
     8641 atcgcaaaaa aatatggtct gtcctacaat gcacttatgg taatgtatat tatcgctgaa
     8701 gatgaaactg ttactcagaa aaacatttgt gatattctgt ttctctcaaa atcaacggta
     8761 cacggtatct tactcgaatt aataaaaaac aagtatgttg tgcttataag cggtaagaac
     8821 aagaaagaaa aacgtattat ctttacaaat aatggagaag agtttttctc ggcaatactt
     8881 aaagatacgg agatttttga aaaaaaagta ctggatttta ttggtgaaaa ggatactctt
     8941 ttttttctag agcaatcaga gcgtgctgca aactatatgc ttagtaaagt aaatgaaaca
     9001 atcaacagag atgattgata tgaatataga aatcaaagaa cttcaaaaaa aagattttaa
     9061 aaaagtaatt gaatttgcgg ttaaaggcat gcatttcgat agatatacag aaaacaaatg
     9121 ggaacttgat atttatggaa ggtatttttt atatttagag ctgcagcgtg cctcacaggt
     9181 tcttgctgct tatatagaag acagactagt aggtgttttg atggccgata tgaaaggaga
     9241 gaagaaaaaa tatctgtctg tatggagaaa ggtatatctt tttttaatag aaaaagcttt
     9301 aagtgtagga tataaaagcg ggactgataa gtatgatgaa gcgaaccaag aaatgtatag
     9361 caggtatata aagcataaca tccctgatgg agaaatttgt tttcttgctg cggatagtac
     9421 ggtccaagga aagggaatcg gaagttgttt attaaaaagg cttgaagaat tagaacgggg
     9481 aaaaagaata tttctatata ctgatagtaa ctgtacttat caattttatg agcgcaaagg
     9541 gtttaaaaga gtagaagaaa aagagataaa attagaaatt catggtaaag aagttccttt
     9601 agtatgctat ttatacgata agaagctata aatttgttat gaaataaggt ttacagagga
     9661 acatatcatg agcatcctaa agatgcatga aaacggaacg aacatttttc gtattctttt
     9721 aaggtgaaat atgattgatt tttcaataga ggatatgcaa gcaaaaaagc aattatccag
     9781 aaagatcatt gtgtaggtat caaacttcag gaaacatata attacataaa agcctttaat
     9841 catatttttg aacgaaaata aaaaaatgtc ggtacgttga acgcaccgac attttacctt
     9901 gttagagatt cataactctg gaggcaacga agggatttga acccttgagt agcgattttg
     9961 cagaccgccg ccttaaccac ttggctacgt tgcctcgaat acctcaatta ttatatcata
    10021 aatactttag aaaatcaaca ccaagtttat tcgtgattat gttcatgttc atgatcgtgt
    10081 tcgtggtcat gatctttatc aatttcgatt aattttactt ctgaagatat ctcatacgct
    10141 actttacctc tgagtacttc ccaaactatc tcattcataa gcttttggtt tgagtaaacc
    10201 atttctctgg ctctttccgg ctgcatatac catacattct gggcaagaga agtgattgca
    10261 ctgtcgatat cctctttttc tgctttgatg tccttctgtt ttactatgct gtcaacaaca
    10321 gcaagttctt ttatccattt cactgaggat tttttaactt cttcaagcat tttagcttca
    10381 tcattttcat gttttccaag ctcttcttca tacttgttct ctttcttaag actgtcaata
    10441 tatcgctggg cataaaaatc tatcgtttca tcagaaagtt ctacttcaac atagttagca
    10501 agttcgccga ctatataatt ttttatgtag tcgttcttcc agttttcata cgcctgtgac
    10561 ccttcatcgg taagcttttc ttttagttcg acaagagatt ttacatctgc acctattccc
    10621 tggacaaaat cgtctgagat ctcaggaata gttctcttga atacttcaag tatatttata
    10681 gtataggtgt aactcttttc ttcaaatttt ctttcaaagg taactgtttc acctgctttt
    10741 ttgccaacta tctcttttac ctgaggtctt tcatcttctt tataaagaac gtattcactt
    10801 tcttttttat cggttatgac ttttccttcg gcatttatta cagagtaagt tgttctgaca
    10861 aaatcttcaa gctcagcagg accgtctttg ggatcaagaa ttgcattctg gtctaaaagc
    10921 tgattaagcc tgtcctcaac atatttttcc acaacctgta cttttttgac aacttctacc
    10981 tgcatatctg aaaatttagt cgaaagtatc tttggataag catgtatctt gacctctatc
    11041 tctattacac cttcgttttg tgaagaggct tctatcatag gatcaaaaag atttttttct
    11101 tctttttcaa tatcagtcat agccagatca acaagatagt cttttatcca agtgtcaaat
    11161 gattcactca ttcttgcttt tataagtgcc ttaggtgcct ttccctttct gaatccctca
    11221 aaagagtagt gctggttaac ctcggcgacg atttcatttt ctgcttttaa gacttcctcc
    11281 tgcgggaatc ttattaagta tctctttatg ttcatatcct gcgctaaaag ctgcttttcc
    11341 atctttttac ctccattttc tatacttctt aatatttatg cttataaatt atacattgtt
    11401 tatacattaa ttatgttaaa aaaaagtcaa aaatacgttc taattctgaa taaacttatg
    11461 taaaacatat ttattcgaat caggttccaa aagcacacaa ctggcaatac ctctgaccca
    11521 cccgcaggct tctcccggat ttattataag ggttttccca tgctgtctaa aaacaacctc
    11581 atgtgtatga ccaaaaaaaa cataatcgta tagccctgaa ctttcagcag gcataagaga
    11641 gtatggctca tgcatcataa agattttctt tccctggtgt tctatagcgg ttggtccata
    11701 ggttatcctt ccatcagatt tttttgaaag tagtaccttt tccccgtcat tattgccaaa
    11761 aacgccaaaa aagtctacgc ctggaatcat gaagctttta agaacaaagg gagctacaaa
    11821 atcaccgcaa tgaaagatac tatcaacttt gtatttttct acaacataag gtacttcctc
    11881 aacaactttc atattatcgt gagtatcgga tattatcatc cacatattct ttctcctcag
    11941 accttttttc caatccatct tatataacat ataatacctt catttaaaga aatttcaacc
    12001 ttttcctttg ctgcttcatt acttacacca agagtatccc aagcgttaag atacttattg
    12061 gtaagctcat acttaaagcc ccgtagattt aatcccttaa cttctccgcc tataggcaga
    12121 actgtccatg tttcaccctt taaggcagta aaaagccttc tgttttttac ggttcctata
    12181 ttaatataat cttcgtttat ttcactgtca agatcattat atgccgcaag aagatatatt
    12241 cccgccattg tctggtctat gcgtgaacct aaaaccccag aaagtacagc tttattctct
    12301 tgcaaccccc ttgccattat aagagcaagc tcggtatcca gttcgttttt ttcaggagga
    12361 aactttagta tctttactcc gatacttttt gcccattcaa catcgttttc ttctgctgag
    12421 tccatatccc ctataagata cgaaggggct atattgaact tgcggaaaag ttttattccg
    12481 ctatcacagg ctattgtaac cgaagcactt ttgatcatgt ttatataaaa ctcatcactg
    12541 ctcagtaggc ttccgccaga tacaatatat acgatattaa tcaccggcct ttcaatgata
    12601 ttatacagca tcatcttaaa actggcaaga tatatatttt tgtaaaagct tttgtttttt
    12661 tgttataact ttgttataat tttattaact tatctatcgg gaggtgttag aatgaagaaa
    12721 catctgatga tactgttcgt ggtctttatg actactgttg gtttatcgga agcgctttca
    12781 gatttggatc taaaaaacta taacggtgat accgttctga tctccatgac gacattgaaa
    12841 agttcaacag atcttctggc gtacaatggt atggtatata atatttctgc atattctgat
    12901 cttaaaaata tttcaaaaga aaaatttttc agtgtaggaa cagagagtct gcggatactg
    12961 aaaaaagatg cagttatcga aaaaaaactc gcatccgtca aaactatagg ctatctggcg
    13021 gtcagtgtaa aagaacttga aacatttaat ggaaagtcat catcagttcc ggcgtttttt
    13081 gcatcagacg gaaaaattta tgatgtaact gaaaacataa aatgtagggg aggagtacat
    13141 acagttgctt atcaacccgg aaaagatatc actattgacc tcaagaaaag caaacacagt
    13201 aataatgtaa ttaaaggact gcttcttata ggtatactca cttttaactt tgaagatctt
    13261 tacgccatga atggaaaaaa tggaaagcct tctcttgtcg gtgtgaatgg aaaaatctat
    13321 gatttttcag agcttaaacg gtgggcaggc ggcgagcatt taaacgttca tagtgcgggg
    13381 caggaactga cctatcagct taaatcaaaa tctcctcacg gagttaaaaa gatttcttct
    13441 gcttctgtaa tcggactttt ggcaacagat atgaaaggat ttagtactgt tatatctacg
    13501 aataaaaatc ttaagtattc tactaaagaa caaaatatct atgaagagaa agagattata
    13561 ggttttctta caaaatagcc aattatggta atagtctaaa agtaataaat agaaacggac
    13621 tgaagctata ataaacgagt agatacttag tacagcttac aggattatta aaactgacct
    13681 ccttttgggg tcagtttatt atatagatcc gtttttattt tagctaaaca cgtagttaat
    13741 catattttca attatatcaa cagcactctt tttttctttg agattccata tgctacatgt
    13801 aatagttgca acaataggat caaaacaggt attttcaaaa aaagtatagt gaaatttttt
    13861 tttacatata attttttcta tatatctgtt gtaacttttg tatataggat agcttgtgat
    13921 aaaacgattg atagtaggct tttctgatga tgaaaaataa aagtcaataa atttttctct
    13981 ttttgctaga tcaaaaagct tcagatattc ttcagctcgt ttaagccagc ctttttcgta
    14041 ttgagtttca catataatct tttggttttt ccgtattctg gattgtaaag acagatctct
    14101 tatgtgagaa atactttgta ataaaggaag tttgctttca gaaacaaaat tttcagatat
    14161 aaaatatcta tgcccatttt gaaaataagt aatctctttt ttagtaagtt cttcaataat
    14221 ttttttaaaa aaatcttttt ccagagtact tccggatatc tcagcaactg tataaaaaac
    14281 cgacggtaaa ctcatatatt cttgtatatc aaaccaataa cctataagga aagccatttt
    14341 ttgtactagc cttactatgt gctgctcttt tttatattta aggttatatg aaatataggc
    14401 attcattatc ataagtttta aaaaaggaga atctgagctt tgagattcgt ttttagaaag
    14461 ctctatatat tttaatccct gctcgtaatc attttttagt tcaatactgg ctatagcttt
    14521 atgataatat aaaacagcat tacggttttt atctctaaac gggagtaacc gatcaatcct
    14581 tcgtgaatca ttgaataaag acatctctgc aacagccaga ttcattctac agtcatattc
    14641 gagctctttt gaaaggttag aaataacttc ttttaaactt ttatatactc taaaaaaact
    14701 atccttatca cactttgcat caaaaatttt taaaagtacg aaatttgctc taacaaaatg
    14761 ctttagagag ttaaaa
//
LOCUS       DAOSHR010000029        14272 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_3517_length_14272_cov_5.740100, whole genome shotgun sequence.
ACCESSION   DAOSHR010000029 DAOSHR010000000
VERSION     DAOSHR010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 14272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14272
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3517_length_14272_cov_5.740100"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="PLU28_02785"
     CDS             complement(<1..333)
                     /locus_tag="PLU28_02785"
                     /inference="COORDINATES: protein motif:HMM:NF039826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5685 family protein"
                     /protein_id="HOG34023.1"
                     /translation="MYGYISLYFEPTQQEKQLYQYYYCGLCHSLKKYFSEPYRLTIIK
                     EVVLFEIMQQQVQTRKYFKCPYVLFKEKNKSENIQDFKHYAYINSLIVYGKLKDNVNE
                     GKRIPRILI"
     gene            complement(339..1553)
                     /locus_tag="PLU28_02790"
     CDS             complement(339..1553)
                     /locus_tag="PLU28_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34024.1"
                     /translation="MKYFSKSPEEWNDYDQKKKNEKERNNLSHRRIKKYTNLLLIAIA
                     VTAILFMIFGNNIGQRLPNLVSFSGINMNLLAEEVYDFPKALSDVKITVTNISKASKK
                     ISIDNFSFIIINTATAETVYNFVFPQKIETEFSGISSKVLFNLEKEAEVSSIPNGEYE
                     IRANMTINGKNIVLKKFFRYNHELIMNVYTQEEFYVQGEKPVVKFVLSNMTDSKVTKE
                     FFGDISVKSSNKIILKKQITFGSLNLNPMEILELTYQIQEDIPFGQYMIEFNSPSFEK
                     VYTAGLMVAKEKEKDGSQLQLGVTNMSYYSKGELLFLQVSLENKIKNQRYLQVDQVFI
                     QLKNDEKVIFEYNNDTPQRVFISPMVVKKIFDINDFKEIRLERIGNYEIKISVKIQNT
                     FLNKSIRLQVVE"
     gene            complement(1562..2668)
                     /locus_tag="PLU28_02795"
     CDS             complement(1562..2668)
                     /locus_tag="PLU28_02795"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOG34025.1"
                     /translation="MKRKSKFNLYSYHLGKNEVQEASEDVIGEILKKFEDPRITDIHF
                     EPGENVKIRIRYCGKLIDSYELSLEDYKVIVNRLLVLCGLDVVMNHYNADGSFEYEKV
                     TYRVSFIESLRGVSCVIRRLRDISQVFVDLPEDLLDKISGLIGSRTKVIIFSGPTGSG
                     KSTTMSYITGKLKDKYKVHTIENPVEFKIPGTIQIQINKDEDPMEKFKYILRQDPEII
                     VVGELRSSAFAKLLFDSSVSGNSVFTSLHCSNVFEIVSRLRSLGVSDSNISSCVDIML
                     NQRLIPQVCPLCKGIGCPKCYDTGYSGYLNLFEVLYANEDFKNDITLGMNDMELKNKY
                     KNTSDYLNPDVKLQYYFDHKLIDQKTYDELKCSF"
     gene            complement(2652..3758)
                     /gene="ftsZ"
                     /locus_tag="PLU28_02800"
     CDS             complement(2652..3758)
                     /gene="ftsZ"
                     /locus_tag="PLU28_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOG34026.1"
                     /translation="MPFELEMNETEKIFTKKSIYRIKVIGVGGAGNNAVQRMTKKGVH
                     DVELIAANTDVQVLERNDAPIKIQLGKTLTRGLGAGGDPKIGRESAIESVEEVKDLIK
                     GSDLLFIVAGFGGGTGTGAAPIIAKISKELGILTVAIVTTPFHFEGAGRQKIALEGLV
                     NLKENVDSLIKIANDKLLEDENVTVDEGFEKADDVLYQAITGISDLITKPGLVNVDFA
                     DVAAVLRVKGSAMLGIGIAKGKNRAEEAVKMALNSRFLEDQIKNATATLVNISGKNPT
                     LLDIKVVNEFLSQKSIDSEKSKMGVSCIDVPDDMLKVTVVASGYDNIPEEDSNDVYEI
                     PAVYRMFSKSIVTEEITKLNSYLNEHREENEEKE"
     gene            complement(3748..5037)
                     /locus_tag="PLU28_02805"
     CDS             complement(3748..5037)
                     /locus_tag="PLU28_02805"
                     /inference="COORDINATES: protein motif:HMM:NF025806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division FtsA domain-containing protein"
                     /protein_id="HOG34027.1"
                     /translation="MAKNYLIGIDIGSYYTKSILFEVSPDGSYIPIASDKKITDGIVN
                     GEIQDISALNKTIKSVIANFSDKLGKRKESEIVVGYSTNNLNISQEVQAVEFTSRTEI
                     KEEDLHKIKKNIVNKYNEEGKMVLDISFMKFIVDGKVVKNPVSFYVTNSLNVTLNIVW
                     VNENAFALLKNVFKDIVDDSQFPIYDTTLGLCYTATSSNDRNVGVAVLDLGYYQSRII
                     TFKNGMPKLFYSFPYGIKYVLKDISNVLKVPENEANRLLCEQALCMKDTKTVKKVDIR
                     LISGGAVSYTSQNLLNKIVYARTREILNRLNGEIAKFSYEKAQEVGALSGGIVYTGGG
                     AKIKNIDAVLRELVGDNFRSGKLSSNMFVNLAEKYMDDPDLLGVFGIVDRYRFDKAEI
                     GNREQTPVKSGKPQKQAKGGFFNKLIDKIAGGDDDAI"
     gene            complement(5528..7453)
                     /locus_tag="PLU28_02810"
     CDS             complement(5528..7453)
                     /locus_tag="PLU28_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HOG34028.1"
                     /translation="MLDKEYNGNEIKVLKGLEPVRLRPGMYVGSTSKAGLNHLVYEIV
                     DNSIDEYINGYCDSIKVSILKDDVIEVIDNGRGIPVDIHPTEGKSTLEVVMTVLHAGG
                     KFDKRAYKVSGGLHGVGASVVNALSEFMEVTVYTGSDVYYQKYQRGIPVEPVKIIDQS
                     MLTGTKVRFKPDYQIFEDGDIEVESYTIENRLKEIAYLNPPLTIEFSDEKIGKKEVYH
                     FSGGLEEFLEYELKKKKRNKVSSMIYINGFYTYKNDEPDMYADIEFVYTDSDENDLMS
                     YVNNIRTIDGGEHEVGFKTAITRVMNDYARKLGMLKEKDENFTGEDVREGLNAIIHVK
                     LANPTFEGQTKGKLGSKYAREGINQIISEKLQLYFDSHQKECKVMIERVQEATKKRLA
                     AKRARENVKRKSVFESGSLPGKLADCISKDRAISEIFIVEGDSAGGNAKQARDRNYQA
                     ILPLRGKILNVEKTDVEKLLKSEMILNIIAAIGTGISDHFSDEDLRYGKIIIMTDADV
                     DGAHIRTLLLTLFYRYMKKLVENGNIYVAQPPLYRFETGKQYFYLFTEKELENLKEQY
                     KDKKYSIQRYKGLGEMSAQQLWETTMDKENRKLIQIKSEDFESANAVIDILMGEDPQA
                     RRIFIETNAHKIKELDI"
     gene            complement(7453..7758)
                     /locus_tag="PLU28_02815"
     CDS             complement(7453..7758)
                     /locus_tag="PLU28_02815"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HOG34029.1"
                     /translation="MFESFEDILKKLSKKSPVLNKIYILSQLREKWSDVIKDTMLQDT
                     WVQDYEPKSRTLVIGCSNNYEMQEVTLNKLNISEESNRILNASLICEVRAVKRRGKD"
     gene            complement(7759..8583)
                     /locus_tag="PLU28_02820"
     CDS             complement(7759..8583)
                     /locus_tag="PLU28_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HOG34030.1"
                     /translation="MFYNGLEWLEYPLIVGDDPGFMRGYSVYEVVRTYAKAPFGLKKH
                     YERLKKSADFLGIEVPEYSDILRVMNEAMTIHDFSEYRFRLYITPGTSKKKTFYCYVE
                     EIKENHDLTDSGVVINIARERKPVSPVIPYYVKSPLNGYGEYLRRKYDYYYDTLLINE
                     FGYVTECTMSNIFLVSQGVLITPSLKSGILPGITRENILMLASKLSIEVEERNVEIWE
                     LLSAEEVFISHTSRGIVPVRRIIPDFTFSVPGVMTETFISNWADLVLKDPSNWEGF"
     gene            complement(8573..9880)
                     /gene="mtaB"
                     /locus_tag="PLU28_02825"
     CDS             complement(8573..9880)
                     /gene="mtaB"
                     /locus_tag="PLU28_02825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HOG34031.1"
                     /translation="MSFKKISIVTFGCKMNQAESQAIGEKFLELGFDVSYDKDLVELE
                     EGTVIINSCTVTSEAERKIRQYIRKVKRNHPNIKVIVAGCGAYTMPQAFTEAGADLIL
                     GNHEKKHIQDYINEYGIKTDIDYWKKEDDPILVPKESYEGRTRVFFPVQEGCLNSCTY
                     CRIVFARGRKINSIKKNEVIKIISGYIEKGFKEIVLTGINLSYYGFDNDENLEELISL
                     IEKEFCSKSVRIRLTSLYPDSVTNGIIDVLTTSSIFEHHIHYSLQHISDDVLKKMGRR
                     YSASEFFRQAELLRSRDSHFSLTSDIIVGFPGETETDIYKLSEGIKSLDMLKVHVFRF
                     SPRPQTLAASFSAQVLGEDKAERIRFIEKAAFQSREKYLQKFMGMVSDVLVEKDTVSG
                     TTGFGYDQYYVPHHISVSGKHENEFLRVRSISFDDKGVKSIVL"
     gene            complement(9877..10326)
                     /locus_tag="PLU28_02830"
     CDS             complement(9877..10326)
                     /locus_tag="PLU28_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOG34032.1"
                     /translation="MKACELMDKDLTAIFCQDNIERFLMVCQRSGYSALPVVDENYKL
                     IGYLSESTVIKISLPGYLSMMENSAFIPDSTQFIKGLKKVLDHPVSEFISGKPFKVYV
                     DDTAMHVADLMIRNKLKMIPVVDKTNTLVGKIRRITLLWRSAKGDID"
     gene            complement(10342..11307)
                     /locus_tag="PLU28_02835"
     CDS             complement(10342..11307)
                     /locus_tag="PLU28_02835"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOG34033.1"
                     /translation="MSYKMLCVTLAPTLDRKVVIKNFEPGRFFRIVDHADSFVEPGGK
                     GINVAVLLSDFKISSVNMGFLGGSIGSIIQSKLSLYGNITTQFVYIEEETRENIIIID
                     PDNRTLSEINLNGPEISQSDMDYFTSRYRSTLNLVDNVVVSGLIPRGITLNSYANLFR
                     MAKAMNKKTYIEAIGEHFDNVVTNSCPMIVRPDLRKSNLLFGQPLKEDIQEYMKAGSF
                     IISRGAELVIMSYKTVSDIIITKNGGWIFTAKGEIDPAKVKNSGDAFMSGMIYHNIVN
                     GFEPFEAARYGMAFALNSTRYISKKVGSIEEINKSLDDFTIERIG"
     gene            complement(11433..12878)
                     /locus_tag="PLU28_02840"
     CDS             complement(11433..12878)
                     /locus_tag="PLU28_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG34034.1"
                     /translation="MNLMDSMKAMMMKQAAKLVANVVRTGDVDQMAGLFSTLSKLAKE
                     PVKSGLKKMADGAKEHDPMYVSWSKLFQKANPKVVEKVINNLIINEFAVGEKVRQEKM
                     HEYKTVLPKLAVISPTYACNLRCVGCYAALYGHKYQLSKEEMFSIIRQFNDLGIYFFI
                     ITGGEPYFYPYIYDVFKEFNDSFFMTYTNGTLIDDEHAKLLSELGNVTLAISIEGFEE
                     MTDWRRGKGVFNKVKEAWNNLNKYGVIFGASVTATRVNHELLMSDEFWDYLAQNGVAY
                     AWVFQFMPVGADASMDLVPTPEQRYERFRKTDELRLSGKFAFVADFWNHGFLTHGCLA
                     AGSKYLHINAKGYAEPCVFQQFAVDNIREKSIIEILKSPFFEAYKRTIPYSQNLFRPC
                     PIIDNPKVFRSMVNKFNAIAQHPGSERVITDLAPELDKLSEDWKVYADKLWYEEGYSE
                     KYPSKRGVYNYETRMRRYASNEEKLALDKKG"
     gene            complement(12894..13424)
                     /locus_tag="PLU28_02845"
     CDS             complement(12894..13424)
                     /locus_tag="PLU28_02845"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOG34035.1"
                     /translation="MSEKALEMIVEKGLSNFTMDDVAKSCGVAKGSLYTYFKNKDALI
                     ISAFGNLVERLKKVITYEDIKSTDTAEEKAKYFAKTYSQILSDFPSDQFMRLFEILIN
                     STHDQKMMQDLTEIFTDYYKSLLQVWESLFKSKSKALLFQAMFDGLAMYKAVGVSLPP
                     EEFESTFKEIILKIAK"
     gene            13801..>14272
                     /locus_tag="PLU28_02850"
     CDS             13801..>14272
                     /locus_tag="PLU28_02850"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOG34036.1"
                     /translation="MAFLKTLRNFFVSVWFFIGFFLYVWVYGSFRLVQAKFIERKHGK
                     DAAVIFLKDVLSSFGKNVFKLLFCKVNVIGKENIPNGKGGLVIISNHQSLLDIPLIVG
                     YVHTAGFVSKKELLKAPVIGSFIKAVGCIPIDRKNPAQAAVALRNLTKSLLNGNK"
BASE COUNT         4151 a         3050 c         2173 g         4898 t
ORIGIN      
        1 aatcagaatt cgaggtatcc ttttaccttc attaacgtta tcctttagct taccgtaaac
       61 tatcagggag tttatatagg cataatgctt aaaatcctga atattttcgg atttgttttt
      121 ttctttgaat agtacataag gacatttaaa atactttctt gtctgaacct gctgctgcat
      181 tatttcaaaa agaacaactt cctttattat ggtcagtctg taaggctctg aaaaatactt
      241 cttcaaactg tgacaaagac cacagtaata atattggtac aattgttttt cctgttgtgt
      301 aggttcaaaa taaagcgata tatatccgta catttatatt attcaaccac ctgcaatctt
      361 atactcttat tcagaaaagt attttgaatt ttgactgaga tctttatttc ataatttcca
      421 attctttcaa gccgtatttc tttaaaatca ttaatatcaa atatcttttt tacaaccata
      481 ggcgatatga atactctttg aggagtatcg ttgttatact caaaaattac tttttcatca
      541 ttcttaagct gtataaaaac ctgatcaacc tgtaagtatc tttgattctt aatcttattt
      601 tccaaactca cctgcagaaa aagcagctct cctttactgt aatagctcat attagttact
      661 ccaagctgaa gctgtgaacc atctttttct ttttcttttg caaccattaa tccggctgta
      721 tatacttttt caaaagaagg agagttaaac tctatcatat actgtccaaa aggaatatct
      781 tcctgtatct gatatgtcag ctcgaggatc tccatcggat taagattcag acttccaaaa
      841 gtaatctgtt tttttaagat gatcttattt gaactcttta ccgaaatatc tccaaagaac
      901 tctttagtca ccttgctgtc tgtcatgttt gaaagaacaa actttaccac gggtttttct
      961 ccctgtacat aaaactcttc ctgtgtatat acattcataa tcagctcatg attgtagcgg
     1021 aaaaattttt ttaaaactat attttttccg ttaatcgtca tattagctct tatttcatac
     1081 tctccgtttg gaatacttga aacttcggct tctttctcaa gattaaagag cactttagac
     1141 gatatacctg aaaattcggt ttcaatcttc tgtggaaaaa caaaattata aacagtttca
     1201 gcagttgcag tattaattat aataaatgag aaattatcaa tgcttatttt ttttgaagcc
     1261 ttggatatat tagtcactgt tattttgacg tcactcagtg ctttaggaaa atcataaacc
     1321 tcctcggcca acagattcat gtttattcct gaaaaagaaa ccaagtttgg tagcctttga
     1381 ccgatattat tgccaaatat catgaaaaga attgccgtta ccgcaatcgc tataagcaaa
     1441 agatttgtat acttcttaat tcttctgtgg gagaggttat ttctttcttt ctcatttttt
     1501 ttcttttgat cgtaatcatt ccattcttct ggacttttgg aaaagtactt catatatcac
     1561 ctcaaaaaga acactttagc tcatcatatg ttttttggtc aatcaattta tgatcaaagt
     1621 aatactgcag cttcacatca ggattcaggt aatccgatgt attcttatat ttgttcttca
     1681 actccatatc gttcatacct agtgttatat catttttaaa gtcttcgttg gcatacagca
     1741 cttcgaaaag gttaagatat ccgctgtacc cggtatcgta acactttgga catcctatgc
     1801 ccttgcagag cggacatact tgaggtatta ggcgctggtt aagcattata tctacacacg
     1861 aggaaatatt actgtcagat actccaagac ttcgcagcct gcttactatt tcaaaaacgt
     1921 tactacaatg aagggaagta aacacgctat ttccgcttac tgaagaatca aaaagcagtt
     1981 tggcaaatgc ggatgaacgg agttctccta ccactattat ttctggatcc tgccttagga
     2041 tatacttgaa cttttccata ggatcctcat ctttgtttat ttgtatttgt atagtaccgg
     2101 ggattttaaa ttcaactgga ttctcaattg tatgaacctt gtacttgtct ttaagctttc
     2161 ctgttatata ggacattgtt gtacttttcc cggagcctgt aggacctgaa aaaataataa
     2221 cctttgttct gcttcctata agtcctgaaa ttttatccag cagatcttcc ggtagatcga
     2281 caaaaacctg tgatatatcc cgaagccttc ttattacaca gcttactcct cttaaggact
     2341 ctataaaaga tactctgtat gttacctttt catattcgaa tgacccatca gcattgtaat
     2401 gattcataac cacatctaat ccgcataaaa caagcagtct gttgactatg accttataat
     2461 cctcaaggga aagctcatat gaatcgatga gttttccgca gtaccttatc cttattttga
     2521 cattttcgcc aggttcgaaa tgtatgtcgg ttattcttgg atcctcaaat ttttttagta
     2581 tctctcctat aacatcctct gaagcttcct ggacttcatt ctttccaaga tggtaagaat
     2641 ataagttaaa cttactcttt ctcttcattt tcctctctgt gctcattaag atatgagtta
     2701 agcttggtta tctcttcggt aactatgctc ttactgaaca tcctgtatac tgcaggaatt
     2761 tcataaacat cattcgaatc ttcttcaggg atattatcgt atccggaagc aaccaccgtt
     2821 acctttaaca tatcgtccgg aacatctata cagcttaccc ccatttttga tttttcactg
     2881 tcaatggatt tttgactcaa aaattcatta accaccttta tatcaagcaa tgtcggattt
     2941 ttaccactta tattaacaag tgtggctgtg gcatttttta tctgatcttc aagaaacctg
     3001 ctgttaagag ccatttttac ggcttcttca gccctattct tacctttggc gattcctata
     3061 ccaagcatag cagaaccttt taccctaaga acggctgcca cgtctgcaaa gtcaacattc
     3121 acaagaccag gcttggtgat gagatctgaa ataccggtaa tcgcctgata caatacatca
     3181 tcagctttct caaagccttc gtcaacagtc acattttcgt cttcaagcaa cttatcgttt
     3241 gcaattttta taaggctgtc tacattttcc ttaaggttta ccagaccttc caaagctatc
     3301 ttctgccttc cagcaccttc aaagtgaaac ggtgtagtca caatcgcaac ggtaagaatt
     3361 cccagttctt ttgatatttt agctattatc ggcgcagctc cggttcctgt tcctccgcca
     3421 aagccggcaa ctatgaaaag aagatcggat ccttttataa gatcctttac ttcttctaca
     3481 ctttctatag ctgattcacg tccaattttc ggatctccgc cagctcccag acctctcgtc
     3541 agggttttac ccaattgaat ctttatcgga gcatcatttc tttcaagaac ttggacgtct
     3601 gtattagccg ctataagctc aacatcatga acaccttttt tggtcattct ctgaactgca
     3661 ttattgccgg ctcctccgac tccgataacc tttatgcggt atattgactt tttggtaaat
     3721 atcttttctg tttcattcat ctcaagctca aatggcatcg tcatcccctc ctgctatctt
     3781 atcgataagt ttattgaaga aaccgccttt agcttgtttc tgaggcttac ctgacttaac
     3841 aggcgtttgt tcacgatttc cgatttccgc cttatcgaat ctgtatctat ctacaattcc
     3901 aaaaactcct agcaggtccg gatcatccat gtatttttct gcaaggttaa caaacatatt
     3961 tgacgaaagt tttcctgaac ggaaattatc tcctacaagc tctctcagaa cggcatcaat
     4021 attttttatc tttgctccgc cgcccgtata gactattccg ccggaaagag ctccgacttc
     4081 ctgggctttt tcgtatgaaa actttgctat ttctccgttg agacgattaa gtatttctct
     4141 tgttctggca taaacaattt tgtttagcaa attctgcgat gtgtacgata ctgccccacc
     4201 ggatatcaat cttatatcaa cttttttaac ggttttagta tctttcatgc agagagcttg
     4261 ctcacataaa agtctgtttg cttcattttc cggaacctta agcacattcg aaatatcctt
     4321 aagaacatac tttatgccat atggaaatga gtaaaaaagc tttggcatac cgtttttaaa
     4381 ggttattatc cttgactggt aatatcccag atcaagaact gctaccccta catttctgtc
     4441 atttgatgat gttgcggtat agcataagcc caatgtagtg tcgtatatcg gaaactgaga
     4501 atcatcaact atatccttaa aaacattttt gagcagggca aaggcattct cgttaaccca
     4561 gactatgtta agggtcacat tcaatgagtt tgtaacataa aaggaaacag ggtttttaac
     4621 aacttttcca tccacgatga acttcatgaa gcttatgtca agaaccattt taccttcttc
     4681 gttgtacttg ttgacaatat tcttttttat cttatggaga tcttcttctt ttatttctgt
     4741 acggcttgta aattcaacag cctgaacctc ctgagaaata tttaggttat tagttgaata
     4801 gcctacaaca atctcagact ctttacgttt tccaagctta tcactaaaat tcgctataac
     4861 agactttata gtcttgttca gcgctgaaat atcctgaatc tcaccgttca ctataccgtc
     4921 cgttatcttt ttatccgaag caataggtat ataactgcca tcgggactaa cttcaaaaag
     4981 aatggatttg gtatagtagc tccctatgtc aatacctatt agataattct ttgccataga
     5041 caccctcctc agccctattt cccagaaaat atctgattat ttattatagt gtactcacca
     5101 ggatttacta tattaaggta attgtaattc ttagctagat aaaaaaaatt atcgtagtca
     5161 taaaatttta tactaacttt attataacag attaccaaca ttttttgaaa atctatgcat
     5221 aaaaaattgt taagggcttt aaggcttttc aaaatcgtta cttcctcatt cctgtaacta
     5281 gaaaaaatta cttccggagc acccgggata tgcatatctg aaataaagga cgcagtcccc
     5341 tcataatcat agtaaaaata tcttttattt cgacttatac agtaatggag atcctctgaa
     5401 gttattaatg gattatctgt gggtctataa taaattttcc ccttagaacc tccggggaaa
     5461 attattaaaa ctatagttat acagtagaga aaaaaaagaa cagccctgaa caccatgaat
     5521 ggcttcatca aatatccagt tcctttatct tatgagcgtt ggtttctata aaaatacgcc
     5581 tggcctgcgg atcttctccc ataagtatat ctatcacagc attggcactt tcaaaatctt
     5641 cggactttat ctggatgagc ttcctgttct ctttatccat agtggtttcc caaagctgct
     5701 gggcgctcat ttcaccaaga cctttatatc tctgtataga atacttctta tctttatact
     5761 gctctttaag attttccagc tccttttcgg tgaaaagata aaaatactgt ttgcctgttt
     5821 caaaacggta aagcggcggc tgcgccacat atatgtttcc attctcgaca agttttttca
     5881 tatatctgta aaacaatgta agcaagaggg ttcttatatg tgcaccgtca acatcggcat
     5941 cagtcattat gattattttc ccataacgca aatcttcatc gctaaaatga tcggatatgc
     6001 ctgtacctat agctgctatg atattcaaaa tcatttcact ttttagaagt ttttccacat
     6061 ctgtcttttc tacattcaga atctttcccc tgagaggaag tatagcctga tagtttctgt
     6121 ctcttgcctg cttagcgttt cctcctgcag aatccccttc gactataaat atttcagata
     6181 ttgcacgatc cttggatata cagtcggcaa gtttgcctgg aagagaacca ctttcaaaaa
     6241 ctgattttct tttaacattc tctctggcac gtttggcagc aagtcttttt ttcgttgctt
     6301 cctgaactct ttcaatcata accttgcatt ctttttgatg actgtcaaag tagagctgga
     6361 gtttttcaga aattatctga ttgatgcctt ctcttgcata ctttgaaccg agctttccct
     6421 tagtctgtcc ttcaaatgtc ggattggcaa gctttacatg aatgatcgca ttaagccctt
     6481 cacgtacatc ttcacctgta aagttctcgt ctttctcctt aagcataccc aactttctgg
     6541 cataatcatt catcaccctg gtaatggcag ttttgaagcc tacctcatgc tctcctccgt
     6601 caatggttct tatattgttt acataagaca tcaggtcatt ttcgtcagaa tcagtataga
     6661 caaactctat atcggcatac atatccggtt catcgttttt atatgtatag aaaccattta
     6721 tataaatcat gctacttact ttatttcttt ttttcttttt aagctcatat tccaaaaact
     6781 cttccagtcc gccggaaaaa tgatatactt cttttttccc tatcttttca tctgaaaact
     6841 ctatagtaag cggaggatta agatacgcaa tctccttaag cctgttttct attgtatagc
     6901 tttcaacttc tatatctccg tcttcaaata tttgatagtc aggtttaaat cttacttttg
     6961 tacctgtaag catcgactgg tctattatct tcaccggttc aaccggaatg ccacgctgat
     7021 acttctgata atatacatcg cttccggtat acactgtcac ttccataaat tccgaaagcg
     7081 cattcactac agatgcccca actccgtgaa gtcctccgga tactttatac gctcttttat
     7141 cgaactttcc gccggcatgc aaaacagtca taacgacctc cagcgtgctt ttaccttctg
     7201 tcggatgtat atccacagga atacctctgc cgttgtcaat aacctcaatg acatcatctt
     7261 tgagtatact tacctttata ctgtcacagt agccgttgat atactcatca atggaattat
     7321 caactatttc ataaacaaga tggttaagtc cggcttttga agttgatcca acatacattc
     7381 caggtctaag cctaacaggc tcaagacctt taagaacttt tatttcattt ccattatact
     7441 ctttgtcaag cattaatctt tccctctcct tttcactgct ctaacttcgc agatgagact
     7501 tgcgttcaat attctgttcg attcttctga aatattaagc ttattgaggg ttacctcctg
     7561 catttcatag ttatttgaac atcctattac aagcgttctg cttttaggct cgtagtcttg
     7621 aacccaagta tcttgaagca ttgtatcttt tataacgtca gaccatttct cacgcagctg
     7681 cgaaagaata taaatcttat tcaatacagg actttttttt gaaagttttt taagaatatc
     7741 ttcaaaactt tcaaacactt aaaagccttc ccagtttgaa ggatctttca gaacaagatc
     7801 tgcccagtta gaaatgaacg tctctgtcat tacccccgga acagaaaatg taaaatctgg
     7861 tattatacgt cttaccggta caattcctct cgaagtatgc gaaataaaaa cctcttctgc
     7921 tgaaagcagc tcccatattt caacatttct ttcctcaact tctattgaga gctttgaagc
     7981 aagcataaga atattttctc tcgtaatacc tggaagtata cccgatttta agcttggagt
     8041 gataagaacg ccttgcgata ccagaaatat atttgacatg gtacattcgg tcacataccc
     8101 aaattcattt attaaaagtg tgtcataata ataatcatat tttcttctca gatactctcc
     8161 gtaaccattt aaaggtgatt taacatagta tggaataact ggagataccg gctttctttc
     8221 ccgtgcaata tttataacta ccccagaatc ggtaagatca tgattttcct ttatctcttc
     8281 cacataacag taaaatgtct tctttttgga agtacccgga gtaatgtaga gtctgaatct
     8341 gtactcagaa aaatcatgga ttgtcatagc ttcattcatt actctgagta tatcggagta
     8401 ttcaggaact tcaatgccca aaaaatctgc agattttttg agtctttcat aatgcttttt
     8461 cagaccaaaa ggtgcttttg cgtaagtcct tactacctca taaacagagt atcccctcat
     8521 aaaaccggga tcatctccaa ctatcaaagg atattccaac cattccaacc cattatagaa
     8581 caatggattt caccccctta tcatcaaaag aaatactcct gacccttaaa aactcgtttt
     8641 catgctttcc agaaacactt atatgatgtg gtacatagta ttggtcatac ccaaaaccag
     8701 ttgtgcctga aacagtatcc ttctccacaa gcacatcaga taccattccc ataaacttct
     8761 gaaggtattt ttcacggctc tggaaagctg ctttttcaat aaacctaatc ctttcagcct
     8821 tatcctctcc caaaacctga gccgaaaaag atgccgctaa cgtctgtggc cgcggtgaaa
     8881 atctgaaaac atgaacttta agcatatcaa gactttttat cccttcagaa agcttgtaaa
     8941 tatcagtttc tgtttctccc gggaaaccta ctattatatc agaagttaaa ctaaaatgag
     9001 aatctctgct cctcaaaagt tctgcctgtc taaaaaattc tgatgccgaa tacctacgac
     9061 ccattttttt aagaacatca tcagagatgt gctgaagtga ataatgaata tgatgttcaa
     9121 aaatcgaaga agtcgtcaac acatctataa ttccgttcgt gacagaatcc ggatatagcg
     9181 acgtaagcct tattctgaca ctcttagagc aaaactcctt ctctataagg cttataagct
     9241 cctcaagatt ttcatcgtta tcaaaaccat aatatgaaag atttatacct gtaagtacta
     9301 tctctttaaa ccctttttct atatatccag aaattatctt gattacttca ttttttttga
     9361 tgctatttat cttccttcca cgggcaaaaa ctatgcggca gtatgtacag ctgttaagac
     9421 aaccttcctg tacgggaaaa aaaactcttg tccttccttc atacgattcc ttcggaacaa
     9481 gtatcggatc atcttctttc ttccaataat caatgtctgt ttttattcca tattcattaa
     9541 tgtaatcctg tatatgtttt ttttcatggt ttcctaaaat aagatcagcg cccgcctctg
     9601 taaaggcctg cggcatagta taggcgccgc atcctgcaac aataacctta atattaggat
     9661 gattcctttt tacttttctt atatactgtc ttatcttcct ttcggcttca gaggtaacag
     9721 tgcaactgtt tattataact gttccttctt ctaattcaac aagatcttta tcataagata
     9781 catcaaaacc aagctctaaa aatttttcac ctatagcctg cgattccgcc tggttcattt
     9841 tacagccgaa agttactata cttatctttt tgaaactcaa tcaatatcac ctttggcgct
     9901 tcgccacaag agagttattc tacgtatctt gcctaccaga gtattagttt tgtctactac
     9961 aggaatcatt ttcagcttat tgcgtatcat cagatcagca acatgcattg cagtgtcatc
    10021 aacatatact ttgaacggtt tacctgatat aaactctgac acgggatgat caaggacttt
    10081 tttaagacct tttatgaact gggtactgtc agggataaaa gcactgttct ccatcatact
    10141 aagatatccg ggaagcgaaa tttttattac ggtactttca cttaaatacc ctataagctt
    10201 ataattttca tcgactacag gcagagcaga atatccgctc ctctgacaaa ccattaaaaa
    10261 acgttctatg ttgtcctgac agaatatagc tgtcagatcc ttgtccataa gttcgcaagc
    10321 tttcatcgca cacctcttat cttatcctat cctctctatt gtaaaatcat caagactttt
    10381 atttatctct tcaatgcttc ctactttttt gcttatatac ctggtagagt taagagcaaa
    10441 tgccattcca tacctggctg cttcaaacgg ttcaaatcca tttacaatat tgtgatagat
    10501 catccccgac ataaaagcat ctccggagtt cttaactttt gccggatcaa tctctccctt
    10561 agcagtaaaa atccagcctc cgtttttggt aataataata tcacttactg ttttataaga
    10621 cataattacc agttccgctc cccgtgaaat tataaaactt cctgctttca tatactcctg
    10681 aatatcttcc ttcaagggct gtccgaaaag aaggtttgat tttctcaggt caggtcttac
    10741 aatcatagga caactgttgg ttacaacatt atcaaaatgt tctcctatgg cttcaatata
    10801 tgtttttttg ttcattgctt ttgccattcg gaataagttg gcatatgaat ttaaagtaat
    10861 tcctctgggt ataagacctg aaaccacaac attgtctact agattaagcg tagatctgta
    10921 ccttgaagta aagtagtcca tatcagactg ggatatttcc ggaccgttaa gatttatctc
    10981 tgaaagagtc ctgttatcag gatctattat gattatgttt tcgcgggttt cttcttctat
    11041 atatacaaac tgtgtggtta tattaccgta aagtgagagc ttagactgaa ttatagaacc
    11101 tattgatccg cctaaaaacc ccatgttaac agaacttatc ttaaaatccg aaagtaggac
    11161 tgcaacattt atgcccttgc ctcccggttc cacaaaagaa tctgcatgat ctactatacg
    11221 aaaaaaacgg ccgggctcaa aattcttaat tacaactttt ctgtccagcg taggagccag
    11281 cgtaacacac aacattttat atgacatttt gtcacctcag aattaaggta taattcaaca
    11341 cacctaaaga aaattatacc ataaagtaca atgaaaatct aaaaatttta ctttaacata
    11401 ataaaagaag acggtaaaaa ccgtcttctt tatcaacctt ttttatccag agctagtttt
    11461 tcttcattag aagcatatct tctcattctg gtctcataat tgtaaacacc cctcttggac
    11521 gggtactttt ctgagtatcc ttcttcatac caaagcttat cggcataaac cttccagtct
    11581 tctgaaagct tatccagttc aggtgcaaga tccgtaatta ctctttcgct tcccggatgc
    11641 tgcgctatgg cattgaactt attcaccata gacctgaaaa cctttgggtt atctattatc
    11701 ggacatggtc tgaagagatt ctgtgaatac ggtatagttc tcttgtaagc ttcaaagaaa
    11761 ggagatttca aaatctcaat tatacttttt tcccttatgt tatctacggc aaactgctgg
    11821 aaaacacatg gttctgcata tcccttagca tttatatgga gatacttcga acctgctgca
    11881 agacatccgt gggttaagaa tccgtgattc cagaaatctg caacaaaggc aaacttacct
    11941 gaaagtctta gttcgtcagt ttttctgaac ctttcatatc tttgctcggg cgttggcaca
    12001 agatccatag atgcatctgc tccaacaggc ataaactgaa atacccatgc ataggcaaca
    12061 ccgttttgtg caaggtagtc ccagaactca tcactcataa gaagctcatg gttgaccctt
    12121 gtggccgtta cggaagcacc gaaaattaca ccgtacttat taagattatt ccatgcttct
    12181 ttaaccttat tgaaaacacc ctttcctctt ctccagtctg tcatctcttc aaaaccctct
    12241 atggagatag caagcgtaac gtttccaagc tctgaaagca gcttagcatg ttcgtcatca
    12301 ataagcgttc cgtttgtata cgtcatgaag aaggagtcat taaactcttt gaaaacatcg
    12361 tatatatatg gatagaagta aggctctcca ccggtgataa taaagaagta tattccaaga
    12421 tcattaaact gcctgattat agagaacatc tcttcttttg aaagctgata cttatgtcca
    12481 taaagagcag cgtagcatcc aacacatctg agattacatg cgtaggtcgg cgatattacc
    12541 gccaactttg gcagcacggt tttgtattca tgcatctttt cctgtctgac tttttcaccc
    12601 accgcaaatt cattgataat aagattgtta ataacttttt caactacttt agggttagct
    12661 ttctgaaaaa gcttcgacca gcttacatac attggatcat gttccttggc tccatcggcc
    12721 attttcttga gaccgctctt cacaggttct tttgcaagct tggacagcgt tgaaaagaga
    12781 cctgccatct gatcaacatc gccggttctt acaacattgg caaccagctt tgctgcctgt
    12841 ttcatcatca ttgccttcat agaatccatt aaattcattc tacacctcca taattacttt
    12901 gctattttca atattatttc tttaaaagta ctttcaaatt cttctggcgg caaacttaca
    12961 cctaccgcct tatacatcgc aagaccgtca aacatcgcct gaaaaagcaa cgccttggat
    13021 ttggatttaa aaagagactc ccatacctga agcaggcttt tgtaataatc ggtaaaaatc
    13081 tctgtcagat cctgcatcat cttttgatca tgagtagaat ttattaatat ctcaaaaagt
    13141 ctcatgaact ggtctgaagg aaagtctgaa agaatctggc tgtatgtctt agcaaaatac
    13201 tttgcttttt cctcggctgt atctgtactt tttatatctt catatgttat tacctttttc
    13261 agacgttcta caagatttcc aaaagctgat attataagag catccttgtt cttgaaatat
    13321 gtgtaaagac tcccttttgc aactccgcag gattttgcca catcatccat tgtgaaatta
    13381 cttaatcctt tttcaacaat catctctaga gctttttccg acaggtactt tttttatctt
    13441 ttttcacctg ttcctgggct tgtaaaacca ttctattatg cctccggaat taacgcttaa
    13501 ttttattgac tgaccgttca gtcaataaaa ttatatgtct caacaattaa aaagtcaaat
    13561 aagatatttt ttatccaatt aaaatccgtt cattatgtaa ctcttgtact tattatgaat
    13621 aatgaattta gtgctaaaaa tatacttttt accactttcg tttaattgaa atataaaatt
    13681 tacattagaa attttatata cataacaaaa aataatattt atcacattaa acatataaag
    13741 ctttttatgg tataataatt ttattggagt tttggattgt tgaggaggaa gttatatatt
    13801 atggcttttt taaaaaccct gagaaatttt tttgtatcag tgtggttctt tataggcttt
    13861 tttctttatg tttgggtata tggttctttt agacttgtcc aggcaaaatt tatagaaagg
    13921 aaacatggaa aagatgctgc ggtgatattt cttaaggatg tactcagctc atttggcaaa
    13981 aatgttttca agcttctctt ctgtaaagtc aacgttattg gaaaagaaaa catccctaat
    14041 ggaaaaggcg gtttagttat tatatcaaat catcagagcc ttcttgatat tcctctaatt
    14101 gtaggttatg tacatactgc aggctttgtt tcgaaaaaag aacttttaaa agcacccgtt
    14161 atcggtagct ttataaaagc tgttggctgt attcccatag acaggaaaaa tcccgctcaa
    14221 gctgcagtag cgctcagaaa ccttaccaaa tcacttttga acggcaacaa ga
//
LOCUS       DAOSHR010000030        14264 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_3523_length_14264_cov_5.294039, whole genome shotgun sequence.
ACCESSION   DAOSHR010000030 DAOSHR010000000
VERSION     DAOSHR010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 14264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14264
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3523_length_14264_cov_5.294039"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      143..314
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            386..1264
                     /locus_tag="PLU28_02855"
     CDS             386..1264
                     /locus_tag="PLU28_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOG34037.1"
                     /translation="MKKFLVSFFVVLLSFVTVFSSVTYVDDLAREVTFDSPVNQVIVA
                     APSISDYLVTLKCEEKVIGITDWDTHIKSEKIGNMVPLNLEKIVSLKPDLVFLTGGFQ
                     EPEVKRLENAGIKAYVINPGTFTDIYKSLMTIGTILGQKEAAAKAANEFREKYINISK
                     QAMNRTNKPKVFYASVYNTVTEIWTAGTGSVVNEMIAYAGGLNVAAPYTGNNGWLSVG
                     PEFILSTNPDIIIVPSYYGDTAGKELLMKTPGLKDVKAVKNGRVYIVDENIVSQTSPS
                     IIKVLEELNKIFGEYK"
     gene            1261..2280
                     /locus_tag="PLU28_02860"
     CDS             1261..2280
                     /locus_tag="PLU28_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOG34038.1"
                     /translation="MRFVKVDRKGFIPLAVFLLGLSFLCIVFFSSFGTVKVPFSDVLK
                     TFLAMPPDKFESSLILRLRIPRIIGSFLAGAVLAVAGNSFQLVIQNPLADPFIIGISS
                     GASFGAVLFTALTVNTAIRIPFGIEGSALIFSVLTSVVVFLLAKQGKKIPILSLVLSG
                     VIISFLLNSLTTMMSVLYWKNVIHVNIWLLGSTADFDWPDIAVVLAFLLLQLLTVFLL
                     SRKLDVLSMNEDLALYSGINPDKLKLAILSVNIAAVSVVVSKSGIIGFVGLIIPHLVR
                     SITGPYSRISTFYCIFIGGIFLSLSDFVSRTFFAPTELPIGVMTSLVGAPVFIYIMKV
                     KGKKV"
     gene            2277..3047
                     /locus_tag="PLU28_02865"
     CDS             2277..3047
                     /locus_tag="PLU28_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG34039.1"
                     /translation="MIKIDQLLFSYESGFTLDIKQLEIKDGEFVSIIGPNGSGKSTLV
                     KLLSGVIRSYRGSIVFDKASIRDLTIKKIAQKVSVVPQEFSTIYEYNVDSIVSSSRIP
                     FAGKFSFSESYEDKKAIEDAMKKTDTLRYKNEVFGNLSGGEKQRVMIARAVAQNTDIL
                     ILDEFVSHLDPGHAQELLKLVKTSNKDAGKTIISVFHDINTASLYSDRIIVFNKGGIF
                     SDGTPYQVITEKMLNEIYALDATIIKHPLYNKPQVIYN"
     gene            3084..4016
                     /locus_tag="PLU28_02870"
     CDS             3084..4016
                     /locus_tag="PLU28_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOG34040.1"
                     /translation="MKTSDLCNIVVNNVAKVIKGKEDKIKLLIAAFLSNGHVLIEDVP
                     GTGKTVISRAISKSIGLGFKRVQFTPDLLPGDLTGLYIYNRKNEEFLLKKGPVFTEFL
                     LADEINRATPRTQSALLEALAERQVTIDGITHQLGKDFFVMATQNPVEFEGTFPLPEA
                     QLDRFMIKTTIGYPEFEDEIKMLSGQDRGHPLNDIQTVVEQVEIDQCKKEVNEVNISP
                     DILSYITRIVDRTRKDKNILLGSSPRGSINLMKISKSIAAIDNRNFVIPDDVKYSAKS
                     VLTHRIILKPETKIRKISPDEIMQNILSEVEVIV"
     gene            4016..5257
                     /locus_tag="PLU28_02875"
     CDS             4016..5257
                     /locus_tag="PLU28_02875"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOG34041.1"
                     /translation="MRTDNLKIKKITLYITIFSFLTLTELSLVLWFILAVLWISFFTK
                     KMAFKKLEVSHSFEYERIFVDEDVCFTVSIKNHSKEEFTLLVTPPHLVDTFFPKEDLF
                     VLGKQEVQKRKFRFSFSERGSKKIGRYTVSFDDRFGLFSFWKVYEVCTEVKVFPKLVS
                     GVFRNQALKKLLPSQKSNVRILEDVSMFENISEYDNEPLNRVHWKASARYDRLMVKKF
                     SFTSMGRTRIYLDANFPNQELILNQAWQELHSQYIEYAIRACGSLIKQMIESNQEVIF
                     KVFSKTYETVDSKNWVEYFDVLSCLEGKYEPIQNFQLYLQEDLRFLTFEDTVIIVSMF
                     LSESILPLLIDLRSRCSKVIVLLMPYGFRLEDEEKTEYVERFRDELLTLQQKAEVLEE
                     NHVLVRLISSNQSLSEVYESV"
     gene            5289..6644
                     /locus_tag="PLU28_02880"
     CDS             5289..6644
                     /locus_tag="PLU28_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34042.1"
                     /translation="MIPFLRFDISDFLLIGVCTVLALKRKILVMAFGIMVQFVVLRYF
                     ILYDHLFVRIAATVLAMLLIYMIPKAEKRILDYYILWISCLIAAKSIIQPELAYLSLI
                     AGAILAFMMFLKSRYVILIMLPIIFLFFFRFSISPMTLALFASSQAPVIKAEENSYQN
                     ENKAFENINGSLSMKAKQTDKANESDTQVLDMLNDIKTKSFLDIFFIGIMMIFFMIIF
                     LTSLKVMPVGKKKTYLSVGSAFLMLVVSITGLLNFNGTLNKLSSDSFAQAHHQASIGS
                     AGKIEDYSEKTEVTDSSSIIKYEKEKQDTKSIIFRYMIVIAAGMVLISILSAAAILKK
                     SVFFFKKQKEPKILNSGEDEYFQSTGTHEIRVYSQHIVKAYHQIRNDYFDSSEDLTPY
                     ELLKLNREWKNFSKLTYEYVNITYGMLDVKYTKEEVKDIVDGAITEVDEFFRQKTKNP
                     A"
     gene            complement(6655..7095)
                     /locus_tag="PLU28_02885"
     CDS             complement(6655..7095)
                     /locus_tag="PLU28_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34043.1"
                     /translation="MFKRLFVLVAVLSLLFCVGFSKMGAGLYLGNPFGLYGRYFLNSS
                     SFVDARISYSGSLAVAADYLLYEPSLVKFEKFVFPLHYGAGATVGFFGSSILLGARVP
                     VGLNYAFMDGKLDVALEAALDIRVIPSFNLGATGGVSVVYYFDK"
     gene            complement(7109..8407)
                     /gene="eno"
                     /locus_tag="PLU28_02890"
     CDS             complement(7109..8407)
                     /gene="eno"
                     /locus_tag="PLU28_02890"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOG34044.1"
                     /translation="MEYIYDEIISVHAREVLDSRGNPTVEAEVVLTSGAKGMAIVPSG
                     ASTGKFEALELRDGDKKRYKGKGTLKAVENINEIISKEIVGLNAFDQVLIDKLMIDLD
                     GTENKEKLGANAILAVSMAVARAAAESLGIPLYRYLGGINSKVLPVPMMNVINGGKHA
                     DSGLEIQEFMIVPAGFSSFKEALRAATETFHSLKSLLSKEGHVTAVGDEGGFAPKFKT
                     NEDAIQHIIKAIEDAGYVPGKDMFIALDCAASEFYNDANEKYTIDKKEVSSDELIAYY
                     EELIKKYPIVSIEDPCNEDDWNGYQKLTSKVGGKVQIVGDDLYVTNVKRLEKGINMRA
                     TNSILIKLNQIGSVTETLDAIEMAKNNNMTCVVSHRSGESEDTFIADLVVATNSGLIK
                     TGSLSRTDRIAKYNQLLRIEEQLGDIGQYRGLKSFYSIKK"
     gene            complement(8432..9136)
                     /gene="pyrH"
                     /locus_tag="PLU28_02895"
     CDS             complement(8432..9136)
                     /gene="pyrH"
                     /locus_tag="PLU28_02895"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOG34045.1"
                     /translation="MKRRVLLKLSGEVLSGEGQKGFNDDAIEYLTQEIKKVSKEGFNI
                     GMVVGAGNLFRGKELDKLTNSIADNIGMLGTVINAIYLKDFFEKQGIKTVVVSQIVSL
                     PSVKPIQYDDIDLYFNSGYVVIFAGGTSNPFFTTDTAAAIRSVEMKADLIVKATKVDG
                     IYTKDPKKYNDALKYDTITYEEAISKNLKVLDTEAFAICQRYNMPIVVLDFFMKDNLI
                     KALKGENVGTRVVEKL"
     gene            complement(9188..9781)
                     /gene="tsf"
                     /locus_tag="PLU28_02900"
     CDS             complement(9188..9781)
                     /gene="tsf"
                     /locus_tag="PLU28_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOG34046.1"
                     /translation="MAITPDMVKQLREMTGAGMMDCKNALVESNGDVNLAVELLRKKG
                     IAKAAKKIDRVTTQGIVYSYIHHNEKVGVLLVLGCETDFVARTQDFHDLAKKISLQIA
                     SMSPRWLSKEDVPEDILSKEKEIYTEEVKSSGKPANIVDKIVENKINKFYEENCLLDQ
                     IYVFGEGEKVKDLITQVIAKIGENIKVDKFTRYAIGE"
     gene            complement(9946..10434)
                     /gene="coaD"
                     /locus_tag="PLU28_02905"
     CDS             complement(9946..10434)
                     /gene="coaD"
                     /locus_tag="PLU28_02905"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOG34047.1"
                     /translation="MSERIAIYPGSFDPVTLGHINIVERTSKIFDHIHVVVMHNPSKK
                     YLFSVEERVKLTGLSLEHLSNVSVDYHCGLMVDYARKKGVKCVIRGLRAVTDFEFELQ
                     MANANRSMCPGLEIFFLMSDVTYSFISSSMVKEVAKFGGDVSTWVSPLIEKELKLKFA
                     QT"
     gene            complement(10427..11545)
                     /gene="mnmA"
                     /locus_tag="PLU28_02910"
     CDS             complement(10427..11545)
                     /gene="mnmA"
                     /locus_tag="PLU28_02910"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOG34048.1"
                     /translation="MRLLNKDKILLLMSGGVDSSVAAYLLKEEGYDVVGLHFKTVPDN
                     FFEATPEKNKICCSPSDTYDAQNVAKQLNLSDFFIVEVHEDFRKKVIDYFLNSYKIGI
                     TPNPCVVCNRYFKFGKAVEIAKEIGAKYVSSGHYVINEYSEHYNAFLLRHGVDSYKDQ
                     SYFLSQIHKEYLNFLYFPLGKLFKSQIREIATSLNLTVANKPDSQEICFIPDENSYRF
                     LRENSVDPGEGKILDLNGKVIGKHKGYTFYTIGQRNGLLFDKAINQRLHVYSIDPINN
                     TLVVAPTEKLYFRSLIANNLNFFTEKNSIKNIVCRIRKKSEEKKASIFFRNDDTAEVV
                     FEEPIFAITPGQFAVFYDYDGFLLGSGEIIKYGEVKNE"
     gene            complement(11529..11969)
                     /locus_tag="PLU28_02915"
     CDS             complement(11529..11969)
                     /locus_tag="PLU28_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOG34049.1"
                     /translation="MGLTVKSSYALRALYELCILNNEGKEKVSITELSRNQNIPRDFL
                     EKIFSELREANILDSLRGRYGGYVLKKKPSDIRLSEIVAVLDKPLQAYDCITDSCKRN
                     VNCAVEFVWKRVYNVMMIELDRITLQDIIDYGTNIEKHGGEASE"
     gene            complement(11971..14187)
                     /locus_tag="PLU28_02920"
     CDS             complement(11971..14187)
                     /locus_tag="PLU28_02920"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HOG34050.1"
                     /translation="MKKGMIFFVILIFSVFVLNGCALFQTPPATSLPEPETKIATVVT
                     YQVIDSDKYVTKEEYQLVLKELAKKNDFESINQSILSFNSRLISLESKINDLAVKSLE
                     LSNRVDIMNVNISQNSFQDTINNIKDSYLIMERRINLLETFIFETKDASSDVNTDIAD
                     IYRRIEKNSTDTALLASKVAALVSAIPAETTKYQIQQSPAVYSFEDFKVSTNKSVEDI
                     NRRYEELYVKMNLLEKTLLNLDAAFKNYKPEVPTPIIQYIEVPVEKDTQQQKSTLPPK
                     VVLPDSLLQSITQNGQTDSISSGSIDRNTEIDALIESVNTINEKIIELENEIKKSNSY
                     LLQNGNMAQMIRDEIAKIDFAPYVDETIDIQTEQAVSKLFYKSQSESILKVQNLEKNI
                     SELQTKYNSISNQFESILSKPVTNFDERMNTKVSELEKRVNIALMSLSDAELKEIFES
                     TDQFVYTLKSGDTLSAISQTFGLGQNGVEILTAANKITNPNTLKVGQKIIIPVDSVEK
                     YIKWPLKTTTPSNYERIVVKFGQRISVGTSTGIGIMPLSNEKIYPILPGKVIDSGKSQ
                     NDTFYVKVDHGNSLVTVYSNLVSLSVSVSSMVTTDTSLGTVKKDKMVTIELWKSGEPK
                     DPLKLFFKSLGTFLATFYTEWDDRLIYYPAFRLTKSGVVPTNFQTIAADPKVLPLGTV
                     VYIPKYAEMPNNGFFVVEDTGGKILGNRIDVYINDVRQAFTTEDVELFIVGKKQEG"
BASE COUNT         4398 a         2554 c         2616 g         4696 t
ORIGIN      
        1 tccaaactga tgaacaatac tatttcttca gcaggaatag gagctaaaat aacagtaccg
       61 atgcttggtc agattagatt tgaatatctt tataagatga atgaaaataa gtgggtaggc
      121 tcatttggat tcggaccggt attctgataa taatcaaaaa attttaaata agcataataa
      181 agggaaaacg gttaaattcc gttacggtgc cgccaccgtg tttggggacg aactccacaa
      241 ttgccactgt atagggaagg cgtggaaagt agattgatcc atcagtcggg atacctttat
      301 atgcctgtac ttcaccattc ctacgcgcca taggggtggt aaagtttttg ggtaatggcg
      361 taatactttt tatactggag gatatatgaa aaagtttttg gtatcttttt ttgttgtatt
      421 actaagtttt gttactgttt tttcttctgt tacatatgta gatgatcttg ctagagaggt
      481 aacctttgat tctcctgtta accaggttat agttgctgct ccttcaattt cagactatct
      541 agtaacgcta aaatgtgagg aaaaggtcat tggaataacc gactgggata cacatataaa
      601 atctgaaaag atagggaata tggtacctct gaatcttgaa aaaatagtat ctttaaagcc
      661 agatctggta tttttgacag gtggatttca ggaaccagaa gtaaagagac ttgaaaatgc
      721 aggtataaaa gcttatgtaa taaatccagg aacctttacc gatatatata agtcactcat
      781 gactataggg actatacttg gtcagaaaga agcagcagcc aaggcagcta atgaattcag
      841 ggaaaagtat attaacatat caaagcaggc aatgaacaga acaaataagc ctaaagtatt
      901 ttatgcttcg gtttacaata cagttacaga gatatggact gcaggcacag gatcagttgt
      961 gaatgagatg attgcatatg caggtggtct gaatgtggca gcgccgtata ccggaaacaa
     1021 tggctggctt tcagtaggac ctgaatttat acttagcaca aatccagata taataattgt
     1081 tccatcatac tatggagata ctgcaggcaa ggaacttctt atgaaaactc cgggtttaaa
     1141 agatgttaaa gctgttaaaa acggaagagt atatattgtt gatgaaaata ttgtttctca
     1201 gacttcacca tctataatta aagtccttga agaattaaac aaaatttttg gagagtacaa
     1261 atgagatttg tgaaagttga caggaagggg tttatacccc ttgctgtttt tttattaggt
     1321 ctttcatttt tgtgtattgt ttttttcagt tctttcggta cggtaaaagt tccattttct
     1381 gatgttctga aaactttttt ggcaatgccg ccggataagt ttgaaagctc cttgattctt
     1441 aggcttagga ttcccagaat aataggaagc ttcttggcag gtgcggtgct tgctgttgct
     1501 ggaaattctt ttcagcttgt aatacaaaat cctcttgccg atccatttat aataggaatt
     1561 tcttcgggtg caagttttgg agctgtttta tttacagctc ttacggtaaa tactgcaatc
     1621 agaattcctt tcgggattga aggatcggct cttatatttt cagttttaac ttctgtggta
     1681 gtatttcttt tagccaaaca aggtaaaaag attccgattc tatctttggt tttaagtgga
     1741 gttataataa gctttcttct aaactctctt actacgatga tgtctgtgct ttactggaaa
     1801 aacgttattc atgtgaacat atggctctta ggcagcactg ctgactttga ttggccggat
     1861 atagcagtgg ttctagcatt tttgcttctt cagctattga cagtcttttt actttcaaga
     1921 aagttggacg ttctttcaat gaatgaagat cttgccttat attctggaat taatccggat
     1981 aagcttaagc ttgccatact ttcggttaac atagcagcgg tttctgtggt ggtttcaaaa
     2041 tcaggcataa taggatttgt gggacttata atccctcatt tggtaagaag tattacaggg
     2101 ccttacagca gaatatcgac gttttactgt atttttattg ggggaatatt tcttagcctg
     2161 agcgactttg tttccagaac attttttgcc ccgaccgaac tgccgatagg tgtaatgaca
     2221 tctcttgttg gtgcaccggt tttcatatac ataatgaaag tgaagggaaa aaaagtatga
     2281 taaaaattga tcagctgtta ttttcatatg aaagcggttt taccctagat attaaacagc
     2341 tcgagataaa agatggagag tttgtatcca taatcggacc gaacggttcc ggaaaaagta
     2401 cgcttgtaaa actgttatca ggagtcataa ggagttacag aggaagcatt gtgtttgata
     2461 aagccagcat aagggatctt acaataaaga agattgccca gaaggtatcg gttgttcctc
     2521 aggagttcag tactatatat gagtataatg tggacagtat agtctcatct tcaagaatac
     2581 cttttgcagg aaagttcagt ttttcagaaa gttatgaaga taaaaaagct atagaagacg
     2641 caatgaaaaa aaccgatact cttagatata aaaatgaagt attcggaaat ctttccggcg
     2701 gagaaaagca aagagtcatg attgcacgtg cagttgccca aaatacagat attctgatac
     2761 ttgatgagtt tgtatcgcat cttgaccccg gacatgcaca ggaactgctt aaacttgtaa
     2821 aaacaagtaa taaggatgct ggtaagacta ttatttcggt atttcatgat ataaatacag
     2881 ccagtcttta ctcggacagg ataattgttt tcaacaaagg cggtatcttt tcggatggca
     2941 caccctatca ggtgataaca gaaaaaatgt tgaacgaaat atacgctctt gatgcaacca
     3001 ttataaaaca tccgctttat aataaaccac aggtgatata taattgatag aaagatatta
     3061 attaataaaa acgggggaag aagatgaaaa catcagactt atgtaatata gtggttaata
     3121 atgtagcaaa ggtgatcaaa ggcaaggagg ataaaataaa actgcttata gcggcctttc
     3181 tcagtaatgg tcatgttttg atagaggatg ttcccggaac cggtaagaca gttatatcta
     3241 gagctatttc aaaatccata ggattgggtt tcaaaagagt tcagtttact ccggatctgc
     3301 ttccaggaga tcttacagga ttatatatat acaatagaaa gaatgaagag tttcttctta
     3361 agaaaggtcc agttttcaca gaatttctat tggctgatga aataaacaga gctactccca
     3421 gaacccagtc ggcacttctt gaagctttgg cagagcgtca agtcactatc gacggtataa
     3481 ctcatcagtt gggtaaagac ttttttgtaa tggctactca aaatcctgtg gagtttgaag
     3541 gaacatttcc tcttccagag gctcagttag atagattcat gataaaaacc acgataggct
     3601 atccggagtt tgaagatgaa ataaaaatgc tcagtggcca ggatagaggc catccgctga
     3661 atgatatcca aaccgttgta gaacaggtag agattgatca gtgcaagaaa gaagtaaatg
     3721 aagttaatat atcgccggat atactttctt atataaccag aatagtagat agaacaagaa
     3781 aggataaaaa tattcttctg gggtctagtc ccagaggaag tataaacctt atgaagatct
     3841 caaagtcaat tgctgcgata gataatagaa attttgttat tccagacgat gtaaagtatt
     3901 ctgctaaaag tgttcttaca caccgtataa ttcttaagcc tgaaaccaaa ataagaaaga
     3961 tttcaccaga tgaaattatg cagaacatac tttcagaagt tgaagtgatt gtttagtgag
     4021 aaccgataac ttaaaaataa aaaaaatcac tctttatata acgattttct catttctgac
     4081 attaacagag ctttctttag tactctggtt tattcttgca gtgctgtgga taagtttttt
     4141 tacgaagaaa atggcattta aaaagcttga agtatcccac agttttgaat atgaaaggat
     4201 atttgtcgat gaagatgttt gttttacagt aagtataaag aatcattcca aagaggagtt
     4261 cacactgctg gtgactccac ctcatcttgt agatacattt tttcctaaag aagatttgtt
     4321 tgtactagga aaacaggaag tacaaaaaag aaagtttaga ttttcatttt ctgagcgcgg
     4381 aagtaaaaaa ataggaagat ataccgtttc ttttgatgat cggtttggtc ttttttcttt
     4441 ttggaaggtc tatgaagtct gtacagaagt aaaagtattt cccaagcttg tatctggagt
     4501 atttcgtaat caggctctaa agaagcttct tccatctcag aagtcaaatg taagaatact
     4561 tgaagatgtg tcaatgtttg aaaatataag tgaatacgat aatgaaccgc ttaacagagt
     4621 tcattggaaa gcgagtgcga gatacgatag gcttatggtg aagaagtttt catttacaag
     4681 catgggtaga acaagaatct atcttgatgc caactttcca aaccaggagc ttattcttaa
     4741 tcaggcttgg caagagcttc attctcaata cattgagtat gccatccggg catgtggttc
     4801 attaatcaag cagatgatag aaagtaatca agaggtaatt tttaaagtat tttcaaaaac
     4861 atatgagact gttgattcaa aaaactgggt tgaatacttt gatgtacttt cgtgtcttga
     4921 aggaaagtat gagcctatac agaactttca gctatatctt caggaggatt tgcgtttttt
     4981 gacatttgaa gatacagtaa tcatagtatc tatgtttctt tcagaaagta ttcttccgct
     5041 tttgatagat ttaagatcgc ggtgctctaa ggtaatagtc ctgctcatgc cttatgggtt
     5101 cagattagag gatgaggaaa aaactgagta tgtcgaaaga ttcagagatg aactccttac
     5161 tttacagcaa aaggcagaag tacttgagga aaatcatgtt ttggtgaggc tgatttcttc
     5221 aaatcaaagt ctttcagagg tctacgaaag tgtataaaca gtattctctt atattgccgg
     5281 tacttataat gattccattt ttaagatttg atatttcaga ttttctactt ataggtgtat
     5341 gtactgttct ggcgttaaag cgaaagattc tggtgatggc ttttggtatt atggtacagt
     5401 ttgttgttct gcggtacttt atactgtatg accatctttt tgtacgtatc gcagcgacag
     5461 tattggctat gctgcttata tatatgattc ctaaggcaga aaaaagaatt cttgactatt
     5521 acatactatg gatttcatgt ttgatcgcag ctaaaagtat aattcagcct gagcttgctt
     5581 atctttcatt gatagctggt gcgattcttg catttatgat gtttttgaag agcaggtatg
     5641 tgattcttat catgcttcct attatttttt tatttttctt tagattctca ataagtccca
     5701 tgacacttgc attgtttgca tcctcccaag cccctgttat aaaagctgaa gaaaatagct
     5761 atcagaatga aaataaagct tttgaaaata tcaatggaag tttatcaatg aaagcaaaac
     5821 aaacagacaa agccaatgaa agtgacacac aggtactgga tatgcttaac gatattaaaa
     5881 caaaaagttt tttggatatt ttttttatag gaataatgat gatttttttt atgattatct
     5941 ttcttacaag cttaaaggtt atgcctgttg gaaagaaaaa aacctatctt tcagtaggca
     6001 gcgctttttt aatgcttgtt gtaagtataa caggactact aaattttaac ggcactctaa
     6061 ataaattatc ttcagatagt tttgcgcagg cacatcatca agcttctatc ggctcggcag
     6121 gaaagataga agattatagc gaaaaaacag aggttacaga cagttcatcc attataaaat
     6181 atgaaaagga aaaacaggat actaaaagta ttattttcag atacatgatt gtaattgctg
     6241 caggaatggt gctcatatct attttgagcg ctgcggctat tttaaaaaaa tcagtctttt
     6301 ttttcaaaaa acagaaggag ccaaagattt taaactctgg agaagatgag tactttcaaa
     6361 gtacaggtac tcatgaaatt agagtttact cacagcacat agtaaaggca tatcatcaga
     6421 taaggaacga ttattttgac tcatccgaag atcttacacc ttatgaactg cttaaactga
     6481 acagggaatg gaaaaacttt tcaaagctta cttatgagta tgtaaacata acttacggta
     6541 tgttagacgt taagtatacg aaggaagaag tcaaggatat agttgatggt gccattacag
     6601 aagtagacga gttctttcgg cagaaaacaa aaaatccggc ttaagccgga ttttctattt
     6661 atcaaagtaa tatactacgc tgactcctcc agtagctccc aaattaaaag aaggaataac
     6721 tctaatgtca agagcagctt ctaatgctac atcaagcttt ccgtccatga aggcatagtt
     6781 cagaccgaca ggaacacgtg ctccaagcaa aatggatgat ccaaaaaatc ctactgttgc
     6841 tcctgcacca tagtgaagcg ggaaaacaaa cttttcaaac tttacaagag acggttcata
     6901 cagaagataa tctgcagcaa cggctaaact accactgtaa cttattcttg catccacaaa
     6961 agaagatgag ttcagaaagt atcttccgta aagaccgaaa ggatttccga ggtaaagacc
     7021 tgctcccatc tttgaaaaac ctacacagaa taaaagactg agtacggcaa caagaacaaa
     7081 caaccttttg aacatcctac acctccgctt atttttttat agaatagaag gatttcagac
     7141 ccctgtactg accgatgtca ccgagctgtt cttctattct taaaagttga ttgtatttgg
     7201 caattctgtc tgtccttgaa agagatccag ttttaataag accggaattg gttgctacga
     7261 ccaggtctgc tataaatgta tcctcagact ctcccgatct gtgagaaaca acacatgtca
     7321 tattgttgtt tttagccatt tcgatagcat caagagtttc agtcacagag cctatctgat
     7381 ttagttttat gagtatagag tttgtagctc tcatattgat gcctttttca agtctcttga
     7441 cattggttac atataaatcg tcacctacaa tctgaacttt tccccctact ttggaggtga
     7501 gcttctgata gccattccag tcatcttcgt tacatggatc ttctatggat acaataggat
     7561 acttcttgat aagttcctca taataagcga taagctcatc agaagacact tcttttttat
     7621 ctattgtgta cttttcattt gcgtcattat aaaattcaga ggctgcacag tccagagcaa
     7681 tgaacatgtc ttttccagga acataaccgg catcttcaat ggccttgatt atatgctgaa
     7741 tagcatcttc gtttgttttg aacttgggtg caaatccgcc ttcatctccg actgctgtaa
     7801 catgaccttc ttttgatagc agagatttta gagagtggaa agtttctgtt gcggctctta
     7861 atgcttcctt gaaagagctg aaacctgctg gaactatcat gaactcctgt atttctaaac
     7921 cgctgtcagc atgttttcca ccgtttataa cgttcatcat aggaacggga agaactttag
     7981 agttaatacc gccaagatat ctgtataacg gtatacctaa ggattcagcg gcagctcttg
     8041 ctacagccat agatacagcc aggatcgcat tagctccaag cttttccttg ttctcggttc
     8101 catccagatc tatcatcagt ttatcaatca gtacctggtc aaaagcatta agtcctacga
     8161 tttccttgga tattatctcg tttatgtttt ctacagcttt aagagtgcct tttcccttgt
     8221 atcttttctt gtcaccatct ctgagttcaa gagcctcaaa cttgccggtt gacgctccgg
     8281 atgggactat tgccattcct ttggctcctg aagtaaggac aacttctgct tcaacggttg
     8341 gatttcccct ggaatcaaga acttctctgg catgtaccga aataatttca tcgtaaatat
     8401 actccatcta aaagcacctc ctgacatatg attaaagttt ttcaactact cgcgttccta
     8461 cattttcacc ttttaatgcc ttaataagat tatccttcat gaaaaaatca aggacaacta
     8521 tcggcatatt gtatctctga catattgcaa aagcctctgt atcaagaacc ttaagatttt
     8581 ttgaaatagc ttcttcatat gtaattgtat catatttcaa agcatcatta tatttttttg
     8641 gatcttttgt atatattccg tccaccttgg ttgcttttac aataagatca gctttcatct
     8701 caactgaacg tatagcagca gctgtgtcag ttgtaaagaa agggttagaa gtaccgcccg
     8761 caaaaattac aacatatcct gagttgaagt acagatcaat atcgtcgtat tgaattggct
     8821 taacagatgg aagagataca atctgtgaaa caactacggt ttttattcct tgcttttcaa
     8881 agaaatcctt gaggtagata gcatttataa ccgttccaag catacctata ttatcagcaa
     8941 tgctgttggt gagcttatca agttcctttc ctctgaaaag atttcctgct cccactacca
     9001 taccaatatt aaagccttct ttagatactt ttttaatttc ctgagtgaga tactctatgg
     9061 catcatcgtt gaaacctttt tgtccttcgc cagaaaggac ttcgccgctg agctttaaca
     9121 gaactctccg tttcataaaa gtacctccaa caaaataaaa gggtggcata tcccaccctg
     9181 atcaaaatta ttcacctatt gcatatcttg taaacttgtc aacctttata ttttcaccga
     9241 tttttgctat tacctgtgtt ataagatctt taactttttc gccctcgcca aaaacataaa
     9301 tctgatcaag cagacagttc tcttcataaa atttatttat tttattttca actatcttat
     9361 ctacaatgtt ggctggtttt cctgaagatt tcacttcttc tgtatagatt tctttttcct
     9421 tactcaaaat atcttccgga acatcttcct tagagagcca tctgggactc attgaagcta
     9481 tctgaaggct tatttttttt gcaaggtcat gaaaatcctg tgttctggca acaaaatctg
     9541 tctcgcagcc caataccaaa agtactccga ctttttcatt atgatgtata taagagtaaa
     9601 ctattccctg tgtggttacc ctgtcaattt ttttcgctgc tttagcaatt cctttttttc
     9661 ttaaaagttc tactgctaaa ttgacatcac cgttagattc cacaagggca tttttgcagt
     9721 ccatcatacc tgctcccgtc atttctctca gctgtttcac catatctgga gttattgcca
     9781 tttttatgtt acctcctcaa agaaaatata tagatttata aaaagattaa aaacatagta
     9841 ccgatataaa aaattgttta aatataaaag ttaagctcta tgtaaaaacc acatcatatt
     9901 ctaacacggg aatttgtcta taaggataaa ttattattaa caaatttatg tctgagcaaa
     9961 tttaagctta agctctttct caataagtgg agaaacccag gtacttacgt ctccaccgaa
    10021 cttagcaact tctttcacca ttgaagaaga gataaaagaa taggtaacgt ctgacatcag
    10081 aaaaaaaatc tccagcccag ggcacataga cctgttagca ttagccatct gcagttcaaa
    10141 ttcaaaatct gtgacagcac gcagtccacg tataacacat ttaacccctt tttttctggc
    10201 ataatcgacc atcagaccgc aatgatagtc taccgataca ttgctcaggt gttcaagtga
    10261 aagaccggta agtttaactc tttcctcaac agagaagaga tactttttgg aggggttatg
    10321 cattacaact acatgaatat gatcaaatat cttggatgtt ctttctacga tatttatatg
    10381 acccaaggtt acagggtcaa agcttccagg atatattgca atcctctcac tcatttttca
    10441 cctctccgta ctttataatt tcaccggagc ctaaaagaaa tccgtcgtag tcatagaaaa
    10501 cagcaaactg gcccggagtt atagcaaaga taggttcttc aaatactact tcggcagtat
    10561 cgtcattacg gaaaaagata cttgcttttt tctcttcaga ttttttccga attctgcata
    10621 caatgttttt gatagaattt ttttctgtga aaaaattcaa attgtttgca ataagtgatc
    10681 tgaaataaag tttttcggta ggagcgacca ctagagtatt gttgattgga tctatactgt
    10741 atacatgcaa acgctgattt atggctttat caaaaagcaa tccgtttctt tgaccaattg
    10801 tataaaaagt ataaccttta tgctttccaa taactttccc gttaaggtct aagatctttc
    10861 cctcacctgg atcaacagag ttttcacgga gaaatctata actattttca tccggaataa
    10921 agcatatttc ctgactgtct ggcttatttg ccacagttaa attaagagat gtagcaattt
    10981 ctcgtatttg tgacttaaaa agctttccca atggaaagta aaggaagtta aggtattctt
    11041 tatgaatttg agataaaaag tatgattgat ctttatagct atccacaccg tgtcttaaaa
    11101 gaaaagcgtt gtaatgctct gagtattcat ttattacata atgaccgctt gaaacatact
    11161 ttgcaccaat ttccttggct atttcaacag ccttgccgaa tttaaagtat ctattacata
    11221 ctacacatgg attaggggtt attcctattt tataactgtt caggaaataa tcgataactt
    11281 tttttctgaa atcttcatgt acttctacaa taaaaaaatc acttagatta agctgttttg
    11341 caacgttttg tgcatcgtaa gtatcagacg gactgcaaca gatcttattt ttttcagggg
    11401 tagcctcgaa aaaattatcg ggtacggttt taaagtgaag accaactaca tcgtagcctt
    11461 cttctttaag caggtatgct gcaactgaac tatcaacccc tccactcata agcaggagaa
    11521 ttttatcttt attcagaagc ctcaccaccg tgtttttcta tatttgtacc ataatcaata
    11581 atatcctgaa gggttattct atccaactct atcatcataa cattgtaaac tctcttccat
    11641 acaaactcta ctgcgcagtt aacgtttctt ttacagctat cggttatgca gtcatatgcc
    11701 tgaagtggct tgtcaagtac tgctactatt tcgctcagcc gtatatcaga aggctttttt
    11761 ttcagaacgt atccaccata tcttcccctt aaggaatcaa gaatattagc ttctcttagt
    11821 tcactaaaaa ttttttcaag aaaatcacgc ggaatatttt gatttcttga aagttcagtt
    11881 atagacacct tttcttttcc ttcgttatta aggatacaaa gttcatataa agctctgaga
    11941 gcatagctac tttttacagt cagccccatt tcatccctcc tgttttttac ctacgatgaa
    12001 tagttcgaca tcttctgttg taaatgcttg tcttacatcg tttatatata catcgattct
    12061 atttccgagt atctttcctc cggtatcttc cacaacaaaa aaaccgttgt tgggcatttc
    12121 tgcatatttg ggaatataga cgactgtccc cagcggaaga acctttggat ctgccgcaat
    12181 tgtttggaag tttgtgggaa caaccccgga tttggtcaat ctgaatgccg gatagtatat
    12241 aagccggtca tcccattcgg tataaaaagt agccaggaat gtacctaagg atttgaaaaa
    12301 aagcttaagc ggatctttag gttcaccgga tttccaaagt tctattgtaa ccattttatc
    12361 ttttttaaca gttcctagtg aagtgtctgt agttaccatt gaagataccg aaacgcttaa
    12421 tgaaacaagg ttagagtaaa ctgtaaccag tgagtttccg tggtcaacct taacataaaa
    12481 tgtatcgttc tgacttttac cgctgtcaat aactttacct ggcagaatag gatatatctt
    12541 ttcattgctt aaaggcatta ttcctatgcc tgtagaagta ccgaccgata tgcgttggcc
    12601 gaacttaaca acaattcgtt cgtaattcga cggtgtggtc gtttttaaag gccattttat
    12661 atacttttca acactgtcta caggaataat tatcttctgt ccaactttca gagtgtttgg
    12721 gtttgtaatc ttattagccg cggtcaggat ttcaactccg ttctgaccaa gtccaaacgt
    12781 ctgagatatt gcactcagag tgtctccact ttttagtgta tatacaaact gatccgtgct
    12841 ttcaaaaatt tctttcagtt ccgcatcact gagactcatc aaggctatat ttaccctttt
    12901 ttcaagctcc gatactttag tattcattcg ttcatcgaag tttgtaacgg gttttgaaag
    12961 aatagattca aactgatttg aaatggaatt atactttgtt tgaagctcgg agatattttt
    13021 ttctagattt tgaaccttaa ggatgctttc agactgggat ttgtagaaaa gcttggaaac
    13081 agcctgttct gtctgaatat ctattgtttc atcaacatat ggagcgaaat ctattttggc
    13141 tatctcatcc cggatcatct gggccatatt gccattctga agaagataac tgtttgattt
    13201 tttgatctcg ttttcaagtt caataatctt ttcgtttata gtgtttacag actctataag
    13261 agcatcaatt tcagtatttc tgtcaatgga tccggaagaa atactgtctg tttgaccatt
    13321 ttgagttata ctctgtagca aagagtcggg taaaacaact ttcggaggaa gagtgctttt
    13381 ctgctgctga gtatcttttt ctaccgggac ttctatatac tgtattatgg gagtcggaac
    13441 ttccggttta tagtttttaa aggcagcatc aaggttaagc agagtttttt caagtaagtt
    13501 catttttaca taaagttctt catatcttct atttatatct tctacagact tatttgtgct
    13561 tactttgaaa tcttcgaaag aataaacggc aggagattgc tgtatctgat attttgttgt
    13621 ttcagcaggg atagcagaaa ccaaggcggc tacctttgat gctagcagag cagtatctgt
    13681 gctgtttttt tcaatcctgc ggtatatatc tgcaatatcg gtgttcacat cagaagaagc
    13741 atcttttgtt tcaaaaataa aagtttcaag cagattaatg cgtctttcca ttataaggta
    13801 actgtcttta atgttgttta ttgtatcctg gaaagaattc tgagaaatat ttacattcat
    13861 tatatctact ctatttgaga gttctaaaga ttttactgca agatcattta ttttgctttc
    13921 aagagatatt agccttgaat taaaggataa gatactttga ttgatgcttt caaaatcatt
    13981 ttttttggca agctctttta aaacaagctg gtactcttct ttagtaacgt acttatccga
    14041 atctataacc tgatatgtaa caaccgtggc aatttttgtt tccggttcag gtaaagatgt
    14101 tgctgggggt gtctgaaaca aggcacaacc atttaatacg aatacagaaa atatcaaaat
    14161 aacaaaaaaa atcatgcctt tcttcacttt atttcccccc tgatggtttt agctgctttt
    14221 atatactgat tccgagagtt tttatacttt gaaactaatt tatc
//
LOCUS       DAOSHR010000031        14193 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_3553_length_14193_cov_5.549583, whole genome shotgun sequence.
ACCESSION   DAOSHR010000031 DAOSHR010000000
VERSION     DAOSHR010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 14193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14193
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3553_length_14193_cov_5.549583"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            453..1487
                     /locus_tag="PLU28_02925"
     CDS             453..1487
                     /locus_tag="PLU28_02925"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOG34051.1"
                     /translation="MFIRTKKIEVDDFNPDISKLKEAAETIKDDSTVVFPTETVYGLG
                     ANAISKNAVQKIFVAKGRPSDNPLIVHISSIEGFKWYAHISDQLLYKVSEFVPGPITF
                     VLEKRDMIPDIVTASRKSVGIRFPAHPVAQRFIELSGFPIAAPSANISGRPSPTNADD
                     VIEDMNGKVDYIITSGNLKFGIESTVVDVTGSAPVILRPGPIPPEKIEEIFGKVIIPD
                     FVYGQSTVDVALAPGMKYRHYSPCVPLVLFEYKQKIDLLQISDNLSFYENPIFLFLEE
                     HSDIFESIGYDKQCIGTISNLYEIGAKIFSMFRKYEKSHDAIIIEGVPDKAIGIAIMN
                     RLRKAASKII"
     gene            complement(1530..2519)
                     /locus_tag="PLU28_02930"
     CDS             complement(1530..2519)
                     /locus_tag="PLU28_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG34052.1"
                     /translation="MKILEAISLKKYFPIRSGVFLQVCGNVKALEKADIKIEQGQTIG
                     VVGESGCGKSTLGKLLLKLYDPTDGKIIYTDKENNSYDITSRISKTVASKFRRDVQMI
                     FQNPFDSLDPRMNIYDIIKEPIAANGMYKNKREMNNYIASLLTGVGLYPEYASRYPHE
                     FSGGQRQRIAIARAMAVMPRLIVCDEPTSALDVSVQSQIINLLKHIQQENRMSLIFIS
                     HNLDVVHHMSDRIIVMYLGNIVESCASEELFNNPLHPYTKTLMSCIPAWNPKEKKLGK
                     TITEGEPPSPINPPNGCPFHPRCQLKMIECEKVKPLPHECEDGHLVACHLYKK"
     gene            complement(2506..3501)
                     /locus_tag="PLU28_02935"
     CDS             complement(2506..3501)
                     /locus_tag="PLU28_02935"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG34053.1"
                     /translation="MTYENKVIDVKNLKTYFRGYDGMIKAVDGISFFLKKGEVLSLVG
                     ESGCGKSVTVNTIMGLIKSPPATVSGEIIYNGKNLVGLKQNEFKNIRGNKISMIFQEP
                     MSSFDPLYTIGQQINEIVKTHITSNNEESKKLIIHALKNVNIPEPEKRYNEYPHEMSG
                     GMLQRILIAMALITNPDIIIADEPTTALDVTIQAQVLKLMKELQKQYNSSIIFITHDL
                     GIVSEIADRVHVMYAGKIIEKASVQEIFDHPLHPYTQGLLNSRVKKEYKGRDLPFIEG
                     YVPKPNEFPNGCRFNPRCPFKKDICTSEEPKESLINEEHSVCCWLYKEGENNENS"
     gene            complement(3498..4589)
                     /locus_tag="PLU28_02940"
     CDS             complement(3498..4589)
                     /locus_tag="PLU28_02940"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG34054.1"
                     /translation="MNSTGKRIMKSFFRHKLGMIGLVILILMYLMVLMADFISPYNYA
                     TTSSGYVNAPPTKIHFFDESGKFRGPFIYALKKSRDPVTYMLSFTEDKSRIVPVKFFV
                     KGDEYSFWGIWKTNIHLFGVEEPSSSAMLLLFGADRFGRDLFSRTMAGSKVSMTVGLI
                     GTFISVVIGAIIGALSGFYGGWIDILLQRFIELLRSFPRIPLWLAFATILPPNWPSTW
                     VYMGIVAVLSLIGWMGIARVVRGMVLSLREKDFVLAAKISGVSGYKIVIKHLIPNTLS
                     YLIVVTTLSIPGMILGESSISFLGLGIKEPMTSWGLLLKQAQSLSALQTTPWLVIPGI
                     FIMLAVLSFNFVGDALRDAVDPYRTVDKL"
     gene            complement(4600..5568)
                     /locus_tag="PLU28_02945"
     CDS             complement(4600..5568)
                     /locus_tag="PLU28_02945"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG34055.1"
                     /translation="MWSFIVRRVLIIIPMMFIISILCFAITELQPGDFLTQYLDNPRI
                     SAEQIELMRHRYGLDKPAYERYFMWIGNILTKGDFGYSFAFERPVGDLIWERMGWTVI
                     IALLTMIFQWILAVPMGIYSALHPYSVGDYSLTVLGFVGLSIPDFFLALLFMIFMLNI
                     GSTNIGGLFSPQFIGASWSFAKFWDLLGHLWMPLVVVGLGGLAGLMRVMRGNMLDVVG
                     SPYITALRAHGLDEKTVKRHAIKNALNPMVSIAGAELPNVFSGTIIIAIVLNIPTMGP
                     FFYSALLNHDQYLVMTFLMFIALITQIANLFADIALAMLDPRIRMN"
     gene            complement(5632..7377)
                     /locus_tag="PLU28_02950"
     CDS             complement(5632..7377)
                     /locus_tag="PLU28_02950"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOG34056.1"
                     /translation="MKRFLSVLLLTVAFFTMCLAAPFVEDSVLTAQGSKNGGTLRLAL
                     PSAPQSFNFYGVLDNSAYTVINQFLAGLVDLHPVTNVVRPGLAESWEFSKDYMEITFK
                     LRDVKWSDGVPFTADDVLFTMDYFVMNKVAKGNSVDRFTAAGKQVEWVKINPKTVKAV
                     LPAPMPGIFQSLAQANIYPKHKLEAKIDKSNPDSVNKVWLTDTPTKDFASIGPYMLSD
                     YIVDQKVVLKKNPNYWKVDSKNNKLPYFDSLEYLIVKDAEVRTAMFMSGQLDWMDVEA
                     TAYPTLKQKELSGAPFTIFLAEPTKNVPSPVHIAFNFDASNINLKNLFSNTKFRQAME
                     FALDRYRIIDEVYQGLAVLGGVPVLPSNKAFYNPKIESIRRGFDLSKAATYLDELGIK
                     DTNKDGIREFKDGTKVEFTLLVANSPVDHQDVGLVFKENVEKLGIKINLQVLDATLVG
                     QKFGSGDFEAGIRAFGNQPDPELRKAIWQPGRQLYYFHRTTMNQNTKEPDQTYMYDWE
                     KEVFEMFEKGAVTMNPELRKSYYDKWQELYAEYLPVIFVCKGKNLYGANKTLGNAYQA
                     KDGLVYFSTWTAYRK"
     gene            7705..8739
                     /locus_tag="PLU28_02955"
     CDS             7705..8739
                     /locus_tag="PLU28_02955"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HOG34057.1"
                     /translation="MNYSFFTRRMENALEMFIDKGIKVSKIFSPEHGIYGLPDGQHIE
                     DSFYGTGRIPVKSLYGKNNYPSQNDYDGLDALIFDIESVGLRFYTFIYTMFYCMKAAA
                     DQGKIFMILDRPNPLGRYVFGKVILPELSSFVGDCFLPASYGLTNGELALYIKKKYSL
                     DLDLRIIKCAGWNGEYFCQGNELWNVPSPALPTFNSLLAYAGFCFFEQTNLSEGRGTY
                     KPFEYIGAPWVDSRGLVAYLHNKFPDLKVRERTFVPFDRKYKAELCFGAEFFPSLKDN
                     FFVISHNILQYCFRSGKLLINEGMDKLTADRKFRLSIENSEKFSFDSWEKESKEFMQS
                     NEDILLYGRD"
     gene            8742..10292
                     /gene="nagZ"
                     /locus_tag="PLU28_02960"
     CDS             8742..10292
                     /gene="nagZ"
                     /locus_tag="PLU28_02960"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HOG34058.1"
                     /translation="MMQESKNGKLFMIGIPSAVLDDETKRLLGHIMPGAIILFARNIV
                     SPQQIKKLVDDINTFLGYKPLISIDQEGGIVSRLREGFSVSPGSMALTAVNDPDCIYD
                     VSKILAREMKAIGINWNLAPVVDINCNPKNPGIGVRSYSDSIDKVIENSKQFVNAMHE
                     EGVMTCLKHFPGKGRVSVDAHIDLPILDINEKELMEFELRPFRELACESIMPSHIYIP
                     SIQRVKEPASLSYEVLTVLARQKLGYKGVLVSDDLSMGGVSNYYSPEQAMIMSLKAGM
                     DVLTFCHDPLVQIRVYNYLCSMVQKDETVYERVSESFTRIEKFSEKANDFPNQEYCIS
                     DIGSKKSLELIQKITDRSVTSRISDESILPLKSDDVGKVFSVRLTRQVQVEDGPNEGI
                     PMVARQIGQLFKKEICVIDPEIKLDGIKDLLKDINCSKVNIVFTENAHLKTGQRKLVE
                     ELCKISPKSLIIALRNPYDCFIKGVRNSLLTYGYELVSQSSLLKVLNGQIKPSGKISV
                     KEYNSDEV"
     gene            10296..11213
                     /gene="murQ"
                     /locus_tag="PLU28_02965"
     CDS             10296..11213
                     /gene="murQ"
                     /locus_tag="PLU28_02965"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HOG34059.1"
                     /translation="MFEKLDTEKPNPDTVNLDSMNVYQILEVMNAEDSKVPKAVSECI
                     PQIERTVEFCIKAVKSFARVVYVGAGTSGRLAVLDSAEIIPTFGVDEGIFVPILAGGD
                     KAFVNAVENAEDSSETGENALKQISLKTDDVVIGITASGRTPFVKGALSYAKTVGCST
                     CLICNVSDPRLSDSADIVISAYTGPEVLTGSTRLKAGTAQKLILNMISTATMIKTGRV
                     FGNYMIGVKIMNEKLMDRAIRIVGGIAGISYEKAKQIIIASEKNVPVAILMALTEGER
                     ELCEKTLRTADGNIRMALEKMRQTHKHLI"
     gene            11129..11929
                     /locus_tag="PLU28_02970"
     CDS             11129..11929
                     /locus_tag="PLU28_02970"
                     /inference="COORDINATES: protein motif:HMM:NF019322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOG34060.1"
                     /translation="MRKNAQDSGRKYPDGIGKDETNTQALNLNNIPIYYKIYQILKAR
                     IEKNEYEEKLPTENELCQEFNVSRLTMRNALQELKRERILSSHKGRGTYIIQKKREEE
                     ISSLAGLSEEASQEGRKVSSLVLHNTLISPPSYVFEKFEMPTKGMVLLIERVRYLDDE
                     PYGIEKCYLNPLVDIRILSLVERDFSRLSIYKTLKDEFDIKFEYAQEIIEIARLSQSE
                     LRMLDMKDMNYGLLRHRYTYVAHNKCIEYVESIYRGDKYKLKLVRRSI"
     gene            11926..12783
                     /locus_tag="PLU28_02975"
     CDS             11926..12783
                     /locus_tag="PLU28_02975"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HOG34061.1"
                     /translation="MKVIGMDGGGGSLKVSFISDYEIKSKIYPYGVNLTSMSEDMLIS
                     IFTEVKRDFGQTDVIAGGFSGLGDPIRKRKLLEILDITFPAEKKILMTDVQAVYDIVF
                     GREKGVVIICGTGSIVYGQDGYGNEYRNGGWGHLFADEASAYWFSKEIIRYALLYRDG
                     LCEYDPIFDELLSFFDVKEYFQLVNLQNDKNFKALIASFSAVLLNKDSRLVDMLIENG
                     SEILFKRVKELEKNIKDLKEIIFFGGVFMAPKFSSAISEKLFEYSTKIFKDDVSMQLA
                     KKYYNEYKF"
     gene            12884..>14193
                     /locus_tag="PLU28_02980"
     CDS             12884..>14193
                     /locus_tag="PLU28_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG34062.1"
                     /translation="MRFLLINPWIEDSAAFDYWLKPVGLLYVAAILRRYGHEVFLIDC
                     LDRFDEEVKPFSSSCRKLEYGMGSFVYHEIDKPQIIKDIPRKFKRYGFPEEIFMDKLS
                     TYSKQNIDAVLLTTTMTYWYKGTVDTVEHVRRIFPDTPVFLGGIYTNILPEHARSTFS
                     PLNVKVLPGTGLSPVIELLKYFEQDYDSFDWFEELDPAYDLYKSDLQYAVLLSSIGCP
                     YRCSYCMTPNMWSYKYRSVERIKNNVELVISKKSIKDVVFFDDAFLLHPQLQELLEML
                     STFETRYHLPNGIHAKKVSPLVAELLKKANFKTIRLGYETSDSSLQKKTGGKVSNADL
                     VNAVGNLKNAGFSSTDIGAYIIGNLPQQNKDDIYNAINFCFDLEILPIVNEYTPIPGT
                     KDHYDLLEQEIFSSDTDPFLLNNKYLPFWWRKGIDNVQMQEIKD"
BASE COUNT         4566 a         2447 c         2763 g         4417 t
ORIGIN      
        1 aaaccaaaat ttgatgcatc aaaacctatt ccttctttaa tcaccttttc agaaacataa
       61 ttttttggta agctaacgtg tctgatatta ccgtgtatat caatcatcag aagatccaaa
      121 taatcgatat tcgatatctt tccatcataa atactgagat tcatatgtta cctccttttt
      181 ctgtagtaat aaaattctaa cattgaaaaa ctaaaaaagc tattttacat aaagtaattt
      241 attgactatg ggtattacag aaaaatcaat atcatatttt ttctatgaaa atcaaagaaa
      301 ttcttaattc tgtatgaaaa aactttcatt ttcctgagaa tgttaattga gcacacaatt
      361 taaaaaaata tgaatcaaaa cttcaaataa tgaatatata atatttttgt acgtgcatat
      421 tatgcaggta aaattaatga aggtgattaa ttatgtttat tcgaaccaaa aagatagagg
      481 tagacgattt caacccagat atatccaagc tcaaagaagc agcagaaaca ataaaggatg
      541 attccacggt tgtttttccg actgaaaccg tttatggctt aggagctaat gccataagta
      601 agaatgctgt acaaaaaata tttgtagcaa aaggacgtcc ttctgataat ccattgattg
      661 tacatatttc cagtatagag ggatttaaat ggtatgccca tataagtgat cagctgctat
      721 ataaggtcag cgagtttgta cctggtccga ttacatttgt actggaaaaa cgtgatatga
      781 ttccagatat agttacagcc tctcggaaaa gtgtaggaat aaggttccct gcacatccag
      841 ttgcccaaag gttcatagag ctttctggtt ttcctatagc tgctccaagt gccaatatat
      901 cgggccgtcc tagtccgacc aatgcagatg atgtaataga agatatgaat ggaaaggttg
      961 actatataat tacttcagga aatttgaaat ttggtataga atctaccgta gttgacgtta
     1021 ccggcagtgc tccggttatt ttaagacccg gacccattcc acctgaaaag atagaggaaa
     1081 tttttggtaa agtaattata cctgattttg tttacggcca gagcactgtt gatgtagctc
     1141 ttgcaccggg aatgaagtat agacattaca gtccgtgtgt tccgttagtt ttatttgaat
     1201 ataaacagaa aatagatctt ttacagattt ctgataatct ttcattttat gagaatccaa
     1261 tatttttatt ccttgaggaa cattcagata tatttgaatc aatagggtac gataagcagt
     1321 gcattggaac gattagcaat ttatatgaga taggtgcaaa aatattttcc atgttcagaa
     1381 agtatgaaaa atctcatgat gccataataa ttgaaggtgt tccggataag gcgataggaa
     1441 tagctataat gaatagactg agaaaagctg cttcaaagat aatctaataa agaatgccag
     1501 acaaaagttt ttcttctgtc tggcgttttt tattttttgt agagatgaca agctaccaaa
     1561 tgtccatctt cacattcatg aggtagaggc ttgacttttt cacattcaat catttttaac
     1621 tggcaacgtg gatgaaaagg acagccgttt ggtggattta taggacttgg aggttcacct
     1681 tcggtgatgg tttttccaag ttttttttct ttgggattcc aagcaggaat acatgacatg
     1741 agagtctttg tataaggatg aagtggattg ttaaaaagtt cttcagaggc acagctttca
     1801 acaatatttc caaggtacat tactattatc ctatcgctca tatggtgtac tacatcaagg
     1861 ttatgagaaa taaaaataag cgacatcctg ttttcctgct gaatatgctt taaaagattg
     1921 atgatttggc tttgaactga tacatccaga gcggatgtag gttcatcaca cactattagt
     1981 cttggcatta cggccatggc tcttgcaata gcaattcttt gccgttgacc acctgaaaat
     2041 tcgtgcggat aacgtgatgc gtattccgga taaagtccga ctcctgtaag taatgaagcg
     2101 atatagttgt tcatttctct tttgttttta tacatgccgt tagctgcaat aggttctttt
     2161 attatatcgt atatattcat tcgaggatca agtgaatcaa aaggattctg aaaaatcatc
     2221 tgaacatccc gtcggaattt tgaggcaacc gtcttgctta ttctcgaagt aatatcgtaa
     2281 gagttgttct ctttatctgt gtaaatgatc ttgccatcag taggatcata cagtttaagc
     2341 agtagctttc cgagagtgct tttgccgcat ccagactcgc cgactacccc tatagtctgc
     2401 ccttgctcta ttttgatgtc tgctttttct aatgctttaa cgtttccaca tacctgaaga
     2461 aaaactccgg atcttatagg aaaatatttc tttaacgaga tagcttcaag aattttcatt
     2521 gttttcacct tccttgtaaa gccagcagca aacagaatgc tcttcattaa ttaaagattc
     2581 tttgggttct tctgaagtac atatatcttt tttgaaaggg catcttgggt taaaacggca
     2641 tccattggga aattcatttg gttttgggac atatccctct ataaatggca gatcacgacc
     2701 tttatactct tttttaactc ttgagtttaa aagaccttgg gtataaggat gaaggggatg
     2761 gtcaaatatt tcctgtacag aagctttttc gattattttt cctgcataca taacatgaac
     2821 cctatcagca atttccgaga ctatacccag atcatgagta ataaatatta tagagctgtt
     2881 atactgtttc tgtagctctt tcatcagctt caatacctgt gcctgaatcg tcacatcaag
     2941 tgctgttgtg ggttcatcag caattattat atctggatta gttattaaag ccatagcgat
     3001 aagaattctc tgcagcattc ctccactcat ctcatgagga tactcgttgt atcttttttc
     3061 cggttcaggt atattaacgt ttttgagtgc atgaataatg agttttttag attcttcatt
     3121 gtttgacgtt atatgtgttt ttactatctc atttatctgt tgacctatag tatagagagg
     3181 atcaaaagaa gacattggtt cctgaaagat catagaaatc ttatttccgc gtatgttttt
     3241 aaactcattc tgtttcagtc cgactaaatt ttttccattg tatataattt ctccagatac
     3301 tgttgctgga ggagatttta taagtcccat tatggtattt accgttactg attttccaca
     3361 tccgctttct ccgacaagcg aaagtacttc tccctttttc aagaaaaaag atatcccatc
     3421 cacagctttt atcataccgt catacccgcg gaaatatgtc ttaaggtttt ttacatctat
     3481 tactttgttt tcgtaagtca tagtttatct accgtcctat agggatccac ggcatcacgt
     3541 agagcatcac ctacaaagtt gaatgaaaga acagccagca ttataaatat gcccggaatc
     3601 acaagccatg gagtagtctg taatgcactt agtgactgtg cctgttttaa aagaagtccc
     3661 caacttgtca taggctcttt tatgccaagt ccaagaaaac ttattgagct ctcgccaagt
     3721 atcattcccg gaatagagag cgttgtaact actataagat aacttagagt gtttggaata
     3781 aggtgtttga ttactatctt ataacctgat actccagata tttttgctgc aagaacaaaa
     3841 tctttctccc gaagacttag aaccatgcct ctgaccactc ttgcaattcc catccatcct
     3901 atcaaagaaa gaacagcaac aatgcccata tagacccacg tacttggcca gttaggcgga
     3961 aggatagttg caaaagccaa ccataaaggt attcttggga aagaacgtaa aagttctata
     4021 aatctctgta ataaaatatc tatccagcct ccgtaaaatc ctgacagtgc accaattatt
     4081 gcccctatta caacgcttat aaacgttccg attaacccta cagtcattga tactttactt
     4141 cctgccatag tgcgggaaaa aagatccctt ccaaatctat cagcaccaaa aagaagcaac
     4201 atcgcagacg aggaaggttc ctcaactcca aaaagatgaa tatttgtttt ccatattccc
     4261 caaaaactat attcatctcc cttcacgaaa aattttacag gaactatcct tgatttatct
     4321 tccgtaaaac ttaacatata agtaacagga tctcttgatt tttttaaagc gtatatgaaa
     4381 ggaccacgga acttacccga ctcatcgaaa aagtgtattt tggtcggagg tgcgtttacg
     4441 tatccggagc ttgtggttgc gtagttgtag ggcgagatga agtccgccat aagaaccata
     4501 agatacatca atatgagtat tacaagtcct atcatgccaa gcttatgcct gaaaaaactt
     4561 ttcattatcc tttttccagt actgttcata aaaaacacct caattcattc ttatacgtgg
     4621 atcaagcata gccagagcaa tatcagcaaa tagatttgca atctgggtta taagagctat
     4681 aaacataaga aaagtcatta caagatactg atcgtggtta agcagtgcac tataaaaaaa
     4741 aggtcccatt gttggaatgt taagtactat ggcaattatg atggttccgc tgaaaacatt
     4801 tggaagttca gctcctgcaa tacttaccat aggattcaat gcattcttta tggcatgacg
     4861 tttgaccgtt ttttcatcaa gtccatgagc acgcaaagca gttatatatg gtgaaccaac
     4921 aacatcaagc atatttccgc gcattactct cattaaacct gccaatcctc ctaatcctac
     4981 tactacaaga ggcatccaga ggtggcctag aagatcccag aacttggcaa atgaccaaga
     5041 agctcctatg aactgtgggg agaaaagacc tccaatattg gttgatccta tatttaacat
     5101 gaaaatcata aataaaagcg ccagaaaaaa atcaggtata gataaaccga caaatccaag
     5161 aacagtaaga gagtaatcgc ctacggaata aggatgaagt gcagagtata tacccatagg
     5221 tacagctaga atccactgga aaatcattgt cagaagggca ataattactg tccatcccat
     5281 tctttcccag ataagatctc ctaccggacg ctcaaaagcg aaggaatacc caaaatcacc
     5341 ttttgtcagt atatttccta tccacataaa gtacctttca tatgcaggct tatcaagacc
     5401 gtatctgtga cgcataagct ctatctgctc tgcagatatt cgcggattat caagatactg
     5461 cgtaagaaaa tcaccaggtt ggagctctgt gatagcaaaa cagagtattg agattatgaa
     5521 catcatagga ataattataa gcactcttct tactataaaa gaccacattt ataaagcctc
     5581 ctgtttcttt aaagtatggg gtgtttatac accccatacg tttatatcat attattttct
     5641 gtaagctgtc catgtggaaa aataaaccaa accgtctttt gcctgatatg cattacctaa
     5701 agttttgttg gctccgtaaa gattcttgcc tttacataca aatattactg gtagatactc
     5761 tgcatagagc tcctgccatt tatcgtaata agatttcctc agttcagggt tcattgttac
     5821 cgcacccttt tcaaacattt caaaaacttc tttttcccag tcatacatat atgtctgatc
     5881 tggttccttg gtattttggt tcatagttgt cctgtgaaaa taataaagct gtcttccagg
     5941 ctgccatata gcctttcgga gttcagggtc aggttgattt ccaaaagccc taataccggc
     6001 ttcaaaatca ccagaaccga acttctgtcc aaccaaggtt gcatccagaa cctgtagatt
     6061 gatctttata cctagctttt caacattctc tttaaatact aagcctacat cctgatgatc
     6121 tacaggagag ttggcaacca aaagagtaaa ctcaactttt gtaccgtctt tgaactcgcg
     6181 tatcccgtct ttattagtat ccttgattcc caactcatca aggtaggtgg cagctttgga
     6241 aagatcaaag cctctgcgta tgctttcaat ttttggatta taaaaagcct tgtttgatgg
     6301 aagcacggga acaccaccaa gaactgccag tccctgatat acttcatcga ttattctgta
     6361 tctgtcaaga gcaaattcca ttgcctgtct gaacttagta ttgctgaaaa gatttttcag
     6421 attgatattt gaagcatcaa aattaaatgc tatatggaca ggacttggaa catttttggt
     6481 aggttctgca agaaatatcg taaatggtgc gccagaaagt tctttttgtt tcaacgtagg
     6541 ataagcagtt gcttctacat ccatccaatc aagctgtccg gacataaaca tagctgtacg
     6601 cacttctgca tctttcacta taagatattc aagggaatcg aagtacggaa gcttattatt
     6661 ttttgaatca accttccagt aattcggatt tttcttgaga actacttttt gatcgacaat
     6721 atagtcagaa agcatataag gtcctataga cgcaaagtcc tttgttggag tgtctgtcag
     6781 ccagactttg ttcactgagt caggatttga tttgtcaatc tttgcttcaa gcttatgttt
     6841 ggggtagata ttagcttgtg ccaaggactg gaatataccg ggcataggag ctggtaaaac
     6901 tgctttaact gttttgggat tgatcttaac ccactcaact tgttttccgg cagcagtaaa
     6961 tctgtctacg gaattacctt ttgcaacctt attcataaca aaataatcca tggtaaacag
     7021 tacatcatca gccgtaaaag gtactccatc tgaccatttt acatcgcgca gcttaaaagt
     7081 aatttccatg taatctttag aaaattccca ggattcggcc aagcctggtc ttactacatt
     7141 agtgacagga tgaagatcta caagccctgc caaaaactgg tttataacag tataagccga
     7201 attatccagc acaccgtaaa aattaaatga ctgaggtgca gaaggcagag ccaaacgtaa
     7261 cgttccacca ttttttgaac cctgggctgt aagtacagag tcctctacaa aaggagcagc
     7321 aaggcacatg gtaaaaaaag caactgtcag cagtaaaaca gataaaaaac gtttcataca
     7381 atccctcctt agataaaaat tatcccttgt atacttgcac atcaacaaca cgttttaatg
     7441 ctattatact taacctgaat tatcaaatct gtttagatgt gattatatat gcttagatct
     7501 ggaaaagagt aaatataagt cgatattgaa tgtatttgta tatacattat acaaaaatat
     7561 gtttgacaag catatagata aacatttaga atataagata gtacaaagta agtacatgga
     7621 gggattacta tttggtataa cggtattgac attttagcag aaaatgattt tagacagata
     7681 agaggtaaaa aactgggact tataataaac tattcttttt ttacacgtag aatggaaaat
     7741 gctttggaaa tgtttataga caaaggaata aaggtaagta agatattttc tccggagcat
     7801 ggaatatatg gtctgccaga tggtcagcat attgaggatt ccttttatgg gacaggtaga
     7861 attcctgtaa aaagtcttta cgggaaaaat aattatccat ctcaaaatga ttatgatggt
     7921 ctggatgctt tgatttttga cattgaatca gttggtttaa gattttacac gtttatatat
     7981 acaatgtttt actgtatgaa agcggctgca gatcaaggta agatatttat gattcttgac
     8041 agacccaatc ctttgggaag gtatgtgttt ggcaaagtaa ttcttcctga actttcctca
     8101 tttgtaggag attgcttttt acctgcttca tatggcttaa caaatggaga actagccctg
     8161 tacataaaga aaaagtactc gttagatctt gatttgagaa taataaaatg tgctggatgg
     8221 aatggagagt acttttgtca aggaaatgaa ctttggaatg ttccgtcacc agccttacct
     8281 acctttaact ctttattggc gtatgctggt ttttgctttt ttgagcaaac aaatttgagc
     8341 gaaggaagag gaacatataa accttttgag tatattggtg caccatgggt ggacagcaga
     8401 ggactcgtag cgtatcttca taataagttt ccagatttaa aagttaggga aagaactttt
     8461 gttccattcg atagaaaata taaggcagag ttgtgttttg gagcagaatt tttcccttcg
     8521 ctgaaagata atttttttgt cataagtcat aatatcctac aatactgttt cagatccggt
     8581 aagcttttaa ttaacgaagg tatggacaag cttacagcag atagaaaatt cagactcagt
     8641 atagaaaata gtgaaaagtt cagtttcgat tcgtgggaaa aagaaagcaa ggagtttatg
     8701 cagagtaatg aagatatttt gttgtatggg agggactaat aatgatgcag gagagtaaaa
     8761 acggaaaatt atttatgata ggtattcctt cggcagttct ggatgatgaa accaagcgcc
     8821 tactgggaca tataatgcct ggggctataa ttctttttgc aaggaatata gtaagtcctc
     8881 aacaaatcaa aaagcttgtg gacgatataa atactttttt aggatacaaa cctcttatat
     8941 caattgatca agagggcggg atagtgagcc gtttgcggga aggattttct gtttcccccg
     9001 gttcaatggc tttgactgct gtaaatgatc ctgattgtat ctatgatgtt tctaagatat
     9061 tagccagaga gatgaaagcc ataggcataa attggaatct tgctccggtg gtggatatta
     9121 actgtaatcc taaaaatcca ggaataggag tgagaagtta ttctgattct atagacaagg
     9181 ttattgagaa ctcaaaacaa tttgtgaatg caatgcatga agaaggtgtg atgacatgtc
     9241 taaagcattt tcccggtaaa ggcagggtaa gtgtagatgc tcacattgat cttcctattc
     9301 ttgatattaa cgaaaaagag cttatggaat tcgaactcag acctttcaga gaacttgcat
     9361 gcgaatctat aatgccgtca cacatatata ttccgtctat tcagagagtc aaggaaccag
     9421 cttccctttc atatgaagtc cttaccgtat tggcaaggca gaaactcgga tacaaaggag
     9481 ttttggtatc ggatgatctt tccatgggag gagtgagcaa ttactattca cccgaacaag
     9541 ccatgattat gtctttaaaa gccggaatgg acgttcttac tttttgccat gatcctcttg
     9601 tgcaaattcg cgtatacaac tatctgtgca gtatggttca aaaggatgag actgtttatg
     9661 aaagagtttc agaatctttt acaagaatag aaaagttttc agaaaaagct aatgattttc
     9721 caaatcaaga atactgtatt tcggacatag gatccaaaaa aagtttggag ctgattcaaa
     9781 aaatcaccga taggtcagtg accagcagaa tatctgacga atcaattctt cctctaaaaa
     9841 gtgatgacgt tgggaaggtt ttttcggtaa ggcttaccag acaggttcaa gtagaagatg
     9901 gtcctaatga gggtattcct atggttgcaa gacaaattgg acagctattc aagaaagaaa
     9961 tctgtgtaat agatcctgaa atcaagcttg atgggataaa agatcttttg aaagatatta
    10021 attgttccaa agtaaatata gtttttacag aaaatgcgca tttgaaaacg ggtcagagaa
    10081 agcttgtcga agaactatgt aaaatttctc ccaaatcgct tataatagct ttaagaaatc
    10141 cctacgactg tttcataaaa ggagttagaa actctttgtt gacatatggt tatgaactgg
    10201 tttcacagag ctcgcttctt aaggttttaa atggtcagat caaaccttcc ggaaaaatca
    10261 gtgttaaaga atataacagc gacgaggtgt gagatatgtt tgaaaaactg gatacggaaa
    10321 aacctaatcc tgataccgtg aatctggata gcatgaatgt gtatcaaata cttgaagtga
    10381 tgaatgccga agattctaaa gtgccaaaag cagtttccga atgtatccca caaatagaga
    10441 gaaccgttga attttgtata aaggcagtta aaagttttgc acgagtagta tatgttggag
    10501 caggtaccag cgggaggctt gcagttcttg acagtgctga gattattcca actttcggtg
    10561 tagatgaggg aatttttgtt cccattctag ccggaggcga taaagcattt gtaaatgcgg
    10621 ttgaaaatgc tgaggatagt tctgaaacag gtgaaaatgc tcttaaacag atatctttga
    10681 agacagacga tgtagttata ggaatcactg caagtggtag aacgccattt gtaaaaggtg
    10741 cgctgagtta tgctaaaact gttggttgca gtacttgctt gatatgtaat gtttcagatc
    10801 caaggctttc tgattcggct gatattgtta taagtgcata tactggtcct gaagtgctta
    10861 caggaagtac aagactcaaa gccgggacag cccaaaaact tatactgaat atgataagca
    10921 cagcaactat gataaaaacc ggtcgggttt ttggtaatta tatgattggt gtcaaaataa
    10981 tgaatgagaa gcttatggac agagctataa gaattgttgg cggcattgcc ggtattagtt
    11041 atgaaaaagc aaaacagata ataatcgcat ctgaaaaaaa tgtaccggtt gcaatactta
    11101 tggcacttac ggagggtgaa agagaattat gcgaaaaaac gctcaggaca gcggacggaa
    11161 atatccggat ggcattggaa aagatgagac aaacacacaa gcacttaatc tgaataatat
    11221 accgatatac tataaaatat atcagatatt aaaggccaga atagaaaaaa atgaatatga
    11281 agagaagctt ccaacggaaa acgagctttg tcaagagttt aatgtaagcc gtttgactat
    11341 gcgtaacgca cttcaagaac ttaaaaggga aagaatactt tcttcacata aaggaagagg
    11401 aacctatatt attcaaaaaa agagagaaga agagataagc tctcttgcag gactttcaga
    11461 ggaagcttcc caggaaggaa ggaaagtcag ctcactagtt ctgcataata cactgatttc
    11521 tcctccaagc tatgtttttg aaaaatttga gatgccaacc aagggaatgg ttctgcttat
    11581 agagagagta agatatctcg atgacgagcc ttatggaatt gaaaaatgtt atcttaaccc
    11641 tcttgtggat atacgtatat taagtcttgt tgaaagagat ttttcaagac tttcaatata
    11701 caagactctt aaggatgaat ttgatataaa gtttgagtat gctcaggaga ttatagaaat
    11761 agcaagactc tcacagtctg aattgagaat gttggatatg aaggatatga actacggtct
    11821 tttacgtcat cgatatactt atgtggctca caataagtgt atagagtatg ttgaatctat
    11881 atatagaggg gataagtata agctgaagct tgtaaggaga agcatatgaa ggttatcggc
    11941 atggatggag ggggaggctc gcttaaagta agttttatat ctgattatga gataaagagc
    12001 aagatctatc cttatggggt taatcttacc tcaatgtccg aggatatgct tataagtatt
    12061 tttactgaag taaagaggga ctttgggcag accgatgtta tagccggagg tttttctggt
    12121 cttggtgatc ctatcagaaa acgtaagctg ctggagatac ttgatattac ttttccagca
    12181 gagaaaaaaa ttcttatgac agacgttcag gcggtttatg atattgtttt cggcagggaa
    12241 aagggtgttg tgataatttg cggaacagga tcgatagtct atggacagga tggatatggt
    12301 aatgagtaca gaaatggcgg ttggggacat ctgtttgctg atgaagcaag tgcttattgg
    12361 ttttcaaaag aaataataag atatgctctt ctttaccgtg atggtctttg cgagtatgat
    12421 ccgatttttg atgagcttct aagttttttt gatgtaaagg agtattttca gctggttaat
    12481 cttcaaaatg acaaaaattt taaagcactt atagcctctt tttctgcagt tcttctaaac
    12541 aaagattcca gacttgtaga tatgcttata gaaaatggaa gtgaaattct gtttaaaagg
    12601 gttaaagagc ttgaaaaaaa cattaaggat cttaaagaaa taatattctt tggaggagtt
    12661 tttatggcac caaagttcag cagcgccatt tcagaaaaac ttttcgagta tagtacaaag
    12721 atctttaaag atgatgtttc aatgcaactt gccaagaagt actataatga atacaaattt
    12781 taaacatcag gaaagaaata acatggtaaa attaaataaa tatttttatc gctccttgaa
    12841 cggagcgcac tttattatta ttgtataaaa agggaggaac tagttgagat ttttgcttat
    12901 taatccttgg attgaagatt ccgctgcttt tgattactgg ctcaagccgg tgggattatt
    12961 gtatgtggcg gctatcctaa gaagatatgg acatgaagtt ttccttattg actgtcttga
    13021 caggtttgat gaagaagtaa agcctttttc ttcttcatgc aggaaactgg agtacggtat
    13081 gggaagtttt gtctatcatg agatagacaa accccagatc ataaaagata taccacgaaa
    13141 attcaaaaga tatgggtttc cagaagaaat ttttatggat aagctcagta cttattctaa
    13201 acaaaatatt gatgcagttc ttcttacgac tacaatgacc tattggtata aaggcacagt
    13261 agatacagtt gaacatgtca gaaggatttt tcctgataca cccgtttttc ttggaggcat
    13321 atataccaat attcttcctg agcatgcgcg ttctactttt tcgccgttaa atgtcaaagt
    13381 actccctggt acaggtcttt ctccggttat agagcttctt aaatattttg aacaggatta
    13441 tgactctttt gattggtttg aagagcttga tcctgcgtat gatctttaca aatcagatct
    13501 tcagtatgcg gttctattaa gttcaatagg ctgtccatac agatgttctt actgtatgac
    13561 tcctaatatg tggtcgtata agtacagaag tgttgaacgt ataaaaaata atgttgaatt
    13621 ggtaatttca aagaaaagca taaaagatgt ggttttcttt gatgatgctt ttttactgca
    13681 tccgcagctg caagaacttt tagaaatgtt atcaaccttt gagacacgtt atcatctgcc
    13741 taacggtatt catgccaaaa aagtttctcc tcttgttgcg gagcttctta aaaaagcaaa
    13801 ctttaaaaca attcgtctgg gatatgaaac atcagactct tctttgcaga aaaaaacagg
    13861 cggaaaagtt tcaaatgctg atcttgtaaa cgctgttggt aatcttaaga atgcgggttt
    13921 ttcatccacc gatattggtg catatataat aggtaatctt cctcagcaga ataaggacga
    13981 tatatacaat gcaataaatt tttgttttga tcttgaaata ctcccaatag ttaacgaata
    14041 tactccgata cctggtacca aggatcatta tgatcttttg gagcaagaga tattttcatc
    14101 tgataccgat ccttttttgc ttaacaacaa gtatcttccg ttctggtggc gcaagggtat
    14161 tgataatgta caaatgcagg aaataaagga tta
//
LOCUS       DAOSHR010000032        14136 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_3574_length_14136_cov_5.275762, whole genome shotgun sequence.
ACCESSION   DAOSHR010000032 DAOSHR010000000
VERSION     DAOSHR010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 14136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14136
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3574_length_14136_cov_5.275762"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="PLU28_02985"
     CDS             complement(<1..281)
                     /locus_tag="PLU28_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34063.1"
                     /translation="MRNDLQERFFRLLETESKDDLISFISELLQQEKMKVFDIYDELL
                     IPSLNEWFCQEKEQRLCVWREHVRSSIIRAIIEISYIYLLKERKELLEKG"
     gene            complement(271..1089)
                     /gene="proC"
                     /locus_tag="PLU28_02990"
     CDS             complement(271..1089)
                     /gene="proC"
                     /locus_tag="PLU28_02990"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HOG34064.1"
                     /translation="MKIGIIGIGTIGGIFLEKCLATSFIEKINIYDKENDKIQHYKNN
                     RIKVCKFPCEVVEESENIILAVRPQDSMELFSEISKSDLSGKVIISTMSGVSIQVLRN
                     SLGCSNILRIMPNVACSVGNGVIAADFSESFDENSKPELIQLISKLGKIFIMDERYFP
                     ALTSISGSGPAFIFVIIESFIDAGIKMGLSYDLAYSIVMEVFDGSVEMMRQSKKHPAE
                     LKNKATSPAGTTIEGIYTLENEGLRGTIMKTLIECYNKAKQFDSSLKGGIKLEE"
     gene            complement(1111..2025)
                     /locus_tag="PLU28_02995"
     CDS             complement(1111..2025)
                     /locus_tag="PLU28_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HOG34065.1"
                     /translation="MYEKYLDYSEDVAAALKNHLPIVALESTIISHGMPFPHNIDTAK
                     QVEKTIIDNGAVPATIAVIDGRVKIGLSDDDMKFLATSKDIIKASRLDLPVAAATRMN
                     ASTTVAATMICAHMAGINVFVTGGIGGVHRGAQQTFDISADLQELAHTNVAVVCAGAK
                     AILDLRLTMEYLETFGVPVLGWKTDELAAFYSSKSGIKVNYRVDDEVTAAKIIKAKWE
                     MGIDGGVIIANPIPQEYSMDPREINDAIDRALEEAERNKIFGKKLTPFLLEKIKELTS
                     GESLESNIALIKNNAYVGALIARELNKN"
     gene            complement(2018..2968)
                     /locus_tag="PLU28_03000"
     CDS             complement(2018..2968)
                     /locus_tag="PLU28_03000"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HOG34066.1"
                     /translation="MLESICVIGGINIDMKIRTFEPIIKGTSNPGRIFFSCGGVGRNI
                     AQNLALFGANVILLGAIGKDWYGQQVISETASSGVDISRIKISEKYSTGIYISVDNNT
                     GEMESGFSDMRISDTVDINYITENLDKIDLCKIIIIDTNIDIDVINNILKYMYKKDIQ
                     CIVEPVSCKKSEKLLYLSYKVDFIKPNLQELVHMSSLCGCKATDPLSCCRTLKEKFKN
                     FLVTMGESGVFYYRSEDDYYKTFKSYEIEVKDVNGAGDAFVAGFAYGMSAKKSIDQSI
                     KYATAASCITLMSEKTVSEDISCASIEKFLSERGDNIENV"
     gene            3253..3987
                     /locus_tag="PLU28_03005"
     CDS             3253..3987
                     /locus_tag="PLU28_03005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOG34067.1"
                     /translation="MFSVAQAQEYLNTILNPQDYDDFCFNGVQIEGLSDVSKIAYGVS
                     LNDDFIEKSIEQGAELLLVHHGIFGKDFINLHGVKKKRIEKIIKSNLTLMSYHLPLDM
                     NIPYGNNISVLKKLKLKYLKTIGFGMIGVYEKPIFFNDFEKNLYETFPRSELQIYRNT
                     EMISTVGIVTGDGSGEIEKYEGLVDTFITGSEKEYTRNWVKESGMNFINAGHYNTEIF
                     GISNIANLLKIKYGADIIFIDVPNNL"
     gene            4033..4773
                     /locus_tag="PLU28_03010"
     CDS             4033..4773
                     /locus_tag="PLU28_03010"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HOG34068.1"
                     /translation="MNGHELLYDVKYYEADYKKRVLITSVMNYFQDLVVKDSEKKQIG
                     LDFLKERDMAWVIAKWDIEVKKYPSFKDTLLVKTIPVALKSFYAHRRFEIWNENSELV
                     ISANTLWILINIKTRKPVKIDALFYDAYGLDYENTDKLDFEKLQIPEQFTAQNEFTIR
                     YTDIDTNEHVNNVKFVEWAMQTIPFEVITSKTLHRIQIEYKKEALYGDSVLSCSKMFK
                     KEDLFEFQHLICDSSMEKEFAVLKSTWK"
     gene            complement(4792..5730)
                     /locus_tag="PLU28_03015"
     CDS             complement(4792..5730)
                     /locus_tag="PLU28_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34069.1"
                     /translation="MNEKLNEYLNQVKAYLNTSDNKEDIIKELEAVVLEKAEKKYGQI
                     TDESISKILSEFGSPAEVAKEYSDEQELIDPSYKNYIFMLTNTFFALIIGINLITNVF
                     RISFTSFKPENLFLSILEIIGTCFSTYFMLFGIICFLFFMLTKYKIKNIAAFWEYKDL
                     STFLAVKPPKVGEMISKIIFFSVFTLFVFFGIDAITVLLSKSGLQVINISDKSIFFAF
                     LFVGLFDILVYAVKFYRNTELLDIVKGFFDLILLWFATIKMENFRITDIAEEISKGIS
                     IGLKVAFIVITVLVIIELIVSILRYSINKTKNIMKV"
     gene            complement(5723..6070)
                     /locus_tag="PLU28_03020"
     CDS             complement(5723..6070)
                     /locus_tag="PLU28_03020"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOG34070.1"
                     /translation="MDIQSELMNITSEMNRGFLQLIILTFLDVPSYGYDIIDSLEKIG
                     YKVEENTLYPLLRRLEKKEVLISEWKVVDNKPRKYYSISKYGKELREEIMKIWSIQNL
                     ILQKLSDGRKENE"
     gene            6250..7116
                     /locus_tag="PLU28_03025"
     CDS             6250..7116
                     /locus_tag="PLU28_03025"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOG34071.1"
                     /translation="MDEKLLDSYIQEFLANLKFIKRRSDSTVKEYKKILTSYKKYLIK
                     IGINKVAFLTYLQDISNLSQRTVRLRIIVIRSFLNYLYESNYIQGTNYWKQARTSFPP
                     ESPKGLTEKQVEILFSVIDDSYDEKFFTLLLKTGLRISEALSLKTENILFYSDYAEMI
                     VIGKGNKERILKISLSYAKNLVKEAKKMIFEVDTSGGETYVPTQRTMQRKFKRYTEVA
                     NQLIDFLRSKGENINFISATPHSLRHTCAKNLLTAGMNIEEVRYILGHSNISTTGIYL
                     RTSQQTKIIAEI"
     gene            complement(7138..8172)
                     /locus_tag="PLU28_03030"
     CDS             complement(7138..8172)
                     /locus_tag="PLU28_03030"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG34072.1"
                     /translation="MKESFKVIITNPRYIFFGLKALSYQLRAASLRNKKEKEGVHVPP
                     FLFLSITDRCNLSCIGCFAKAHKRKEKQELSTEKISDIVTQAKRIGISLIFIIGGEPL
                     LRKDIFKITRNNENIIFPVFTNGTLLDEVIFENFKTQKNLIPVLSIEGDQWNTDQRRG
                     KGVFSSLKELSEKFNKNHIIWGASITVTKSNYDQVTSDEYIDALLKKGCKLILFIEYV
                     PTSGSDSLVIDQDQRDHLAKISKEMKKKFNGIFVSFPGDEKEYGGCLSSGKAFLHISA
                     FGDVEPCPFSPYSDCNINEVSLDEALKSDFLHEIRINHDKLEETSSGCALWNNKDWVA
                     SLASQKNHQE"
     gene            complement(8256..8963)
                     /gene="nuoB"
                     /locus_tag="PLU28_03035"
     CDS             complement(8256..8963)
                     /gene="nuoB"
                     /locus_tag="PLU28_03035"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HOG34073.1"
                     /translation="MRIAWDRLKNKKETVKNPISDNKYAFGPVKIDYSNCSYCNSCAE
                     NCFTGAISIENGKIEYDVNKCVFCRRCISTCPNQAITATHDYKLSQLIDNPDEMRKKI
                     FSIYKRSFVLRSVDCGSCNACMSELSALNNTYYDISRYGVTFAASPRHADGIIVTGPV
                     AINMKHALLKTYYAMAEPRIVIACGTCTYENGVYKDGYSVKESLSEILPVDLFIPGCP
                     PSPQAIIYGLFLLLEKL"
     gene            complement(8968..10536)
                     /locus_tag="PLU28_03040"
     CDS             complement(8968..10536)
                     /locus_tag="PLU28_03040"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HOG34074.1"
                     /translation="MHIEDFIREFKKVVFNTDIEIKNSNEAYIYTIQPEVRYIVARLT
                     DDFGLSFMSEFCFEENVFVINVVFSSRKDGFLVVLRYNADGYIDSLTQDIPRAGIYER
                     EISDLYGLKIIGTSDTRCLVKHENWDLNEFPLRKDFDSSRKINEKNNVPNYIFESVKG
                     EDGFQIPVGPVHAGIIEPGHFRFTVCGEPIDNLEIRLMYKHRGIEKICESKNFNLLPV
                     IMERVSGESSAAYALAYALAIEKIMGKTVSPEIKAFRTVIVELERIYNFLEDIAGICT
                     DIAFSYPSKKFSYLSELVHQICEELTNSRFLRSAIIPNGSGIDFAIVDKDTVINKLDK
                     IIKRFKYISDMTVQSVSFLDRVERTGCISMEEGVSLGLTGVVARACGIRYDVRKIFPY
                     NNYNKLFVHEIPSMELGGVYERFQLKVKEIFQSYEFILKSFEEINVDIVKTVPQIKIP
                     DHTEVFSMVETVKGELLLYIRSAKNNYPCRVYFKTPSFSNWSGLEYAVRKEIVPDFPL
                     CNKSFNMSYSENDR"
     gene            complement(10549..10860)
                     /locus_tag="PLU28_03045"
     CDS             complement(10549..10860)
                     /locus_tag="PLU28_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34075.1"
                     /translation="MPPFASFFSELKILNAGFSSNYAIVAIITVFLTVVVFGAFLSHF
                     TRMIFFKDKDTLPDEESLREKETNVFPLMLSLIMIVLASLYFLPFLNKILEKATLIVL
                     G"
     gene            complement(10826..12013)
                     /locus_tag="PLU28_03050"
     CDS             complement(10826..12013)
                     /locus_tag="PLU28_03050"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOG34076.1"
                     /translation="MQVLYFIFIAVISVLFFVLIKNVRYKSYISVLSSIGNLLVVLKI
                     AADIQIYGSISYFTGTYYIDSLSLIQLFIITFVALIASIYSVKYITAEVEEGVISSKK
                     ATLYFILFSIFSISMIFVAISNNMISMWIWLEATTLSTAFLISFDGSKHSLEAAWKYL
                     ILCSLGIAVGLIGILVFIYSAGKVESANVLKWDYLVSISDSLDINLVKIAFTLIFIGL
                     GTKAGLAPMHTWLPDGHSQAPAPISAMMSGALLNLAMYVIIRFYTIISNISALKYMRF
                     LFTVFGLLSLVVAAFSMIKAKNIKRLLAYSSIENIGIISIGIGFGFFDGIYGSLLHSI
                     IHAFAKTLLFLIAGNIIVSYKTKNLSEINGMIYKIPINSVFIIIGMLVNRDATVCFFF
                     QRA"
     gene            complement(12023..12634)
                     /locus_tag="PLU28_03055"
     CDS             complement(12023..12634)
                     /locus_tag="PLU28_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34077.1"
                     /translation="MTDFFLAVILISAILMSGVRRVKLLISGYTVQAFCIFAISFLTA
                     LSTNDKHYYVISIMTLLAKVILVPLILTHSVRKIKTKVELSPVLNSFWSYTLTIISIA
                     LASYISAGSGILYIKEAISLSIVGALLLIGRRKALSQMIGFLVIENGLMLFELSLIKL
                     SFLIEFAIVVEAIILFIIMAIVIGNINAAFDTTNTDYLSDLKE"
     gene            complement(12647..13576)
                     /locus_tag="PLU28_03060"
     CDS             complement(12647..13576)
                     /locus_tag="PLU28_03060"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HOG34078.1"
                     /translation="MMDKNIMMFVNGFLIIILAPLFSGVLKKSKAFLRGYKGPSVFQV
                     YYDIGKLFRKSFIKSKSSSAVTSFAPSAILAFTVVTTFFIPVVYAYPKVQGFSVFLVF
                     FSFTAVKFMTALIGLDCASTFGGMGSSREMFISMMSEPLFLVLIMFLFMETGTFSVEN
                     MSYINSIVINDFIGHIIAASAFIIFLIVENTRVPVDNPETHLELTMVHEAMVLDISSC
                     DLGFLELASSIKLAVFSTFFVNIFFPVGIAISLNPINIFAALFFYFTKMILFLFSLAV
                     IEISTAKFRLFKVQDIIMAGFSLAVISLVISYL"
     gene            complement(13587..>14136)
                     /locus_tag="PLU28_03065"
     CDS             complement(13587..>14136)
                     /locus_tag="PLU28_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34079.1"
                     /translation="ISFAVVLSSLLSLAAGFFSSYIIDVILKIGSSVFGDTLPENFSK
                     NFDSIMIFGFFILMFFIFFVFIRYLLGTFRKKEVSDTWACGHREFEPKFQYTSSGYAQ
                     PASKLFKNAVSYSKKVQQKKGISISISANDLLQDYVYPFFIWTFGVVSRFFHKIQHGY
                     IQLYVLYFAVTLALTIFLVLKG"
BASE COUNT         4882 a         2580 c         2101 g         4573 t
ORIGIN      
        1 cctttttcca aaagttcttt tctttctttt aaaagataga tatatgatat ctctataatt
       61 gctcttataa tactgcttct cacatgttca cgccaaacgc atagacgttg ctccttttcc
      121 tggcaaaacc actcattgag tgaaggaatc aaaagctcat cgtagatatc aaatactttc
      181 atcttttcct gctgtaagag ctctgaaata aaagaaataa gatcgtcttt gctctcggtt
      241 tcgagtaatc tgaaaaacct ttcttgcaga tcattcctca agttttatcc ctccttttaa
      301 tgaactgtca aactgtttgg ctttattata acactctata agagttttca taatagtccc
      361 ccgcagccct tcattttcaa gagtatatat accttctatg gttgtacctg caggtgaagt
      421 ggctttgttt tttaattcgg caggatgctt tttactctga cgcatcattt ctaccgatcc
      481 atcaaatacc tccatgacaa tagagtatgc aaggtcataa gaaagcccca tctttatacc
      541 ggcatctata aacgactcta ttatgacaaa aataaaagct ggaccactac cgcttataga
      601 ggtaagtgca ggaaagtacc tttcatccat gatgaagatc tttcccaatt ttgatataag
      661 ctgaatcagt tccggcttac tgttttcatc aaaagattca gaaaagtccg ctgctatgac
      721 tccatttccg acagaacatg caacgttggg cataatcctc agaatattgg aacatcccag
      781 actatttctg agtacctgaa ttgatactcc tgacatagtg ctgattataa cctttccaga
      841 aagatcactt ttggatattt cagaaaaaag ttccattgaa tcctgaggcc ttacagcaag
      901 tataatattt tctgattctt caacaacctc gcaaggaaac ttgcagacct tgattctatt
      961 atttttataa tgctgaatct tgtcattttc tttatcgtat atatttattt tttctataaa
     1021 agatgtagct aggcacttct ctaaaaatat ccctccaata gttcctatac ctataattcc
     1081 tattttcatc ctatgtcccc cctgttttat ttaattttta ttcagttctc tggcaattaa
     1141 tgcgcctaca taggcgttat tttttattaa tgcgatatta gactcaagac tttcaccaga
     1201 tgtaagttct ttaatttttt ccaataaaaa aggagtaagc tttttaccaa aaatcttatt
     1261 tcgttcagct tcctcaagag ccctgtcaat tgcatcgttt atctcccttg gatccattga
     1321 atactcttgt ggaatcggat tagcaatgat aactccgcca tctattccca tttcccattt
     1381 agcttttatt atttttgccg cagtaacttc atcatcaacc ctataattca cctttattcc
     1441 gcttttactc gaataaaaag cagcaagctc atcggttttc caacctaaga caggtacacc
     1501 aaaagtttcc agatactcca ttgtcaatct aagatcaaga atagcttttg ctcctgcaca
     1561 aacaacagca acatttgtat gagcaagctc ctgcagatca gccgaaatgt caaaagtctg
     1621 ttgagcacct ctgtgtacgc caccgattcc acctgttaca aaaacattta tgccagccat
     1681 atgcgcacat atcattgttg ccgcaacagt tgttgaagcg ttcatccttg ttgctgctgc
     1741 aactggaaga tctaatctgc tggcttttat aatatctttt gaggtagcaa gaaatttcat
     1801 atcatcatca gaaagaccaa tttttactct accgtcaatt actgcaatag tagcaggtac
     1861 agctccgtta tcaattattg ttttttccac ctgttttgct gtatcaatat tatgtggaaa
     1921 cggcatacca tgtgatatta tagtagattc aagtgcaact atcggaagat gattttttaa
     1981 tgcagctgcg acgtcttcag aataatcaag atacttttca tacattttca atattatcac
     2041 ctctttcact aagaaatttt tcaatacttg cacacgaaat atcttctgaa acagtttttt
     2101 ccgacattaa tgttatacat gaggctgctg ttgcgtattt tatcgactgg tcgatgcttt
     2161 tttttgcaga cattccatag gcaaatcctg ccacaaaagc atcaccggca ccatttacat
     2221 ctttaacttc aatttcatac gacttaaaag ttttataata gtcatcttca gacctataat
     2281 aaaatacacc ggactctccc atagtcacaa gaaaattttt aaatttttct tttaaagttc
     2341 tacagcacga taaaggatct gttgctttac agccgcaaag agatgacata tgcacaagct
     2401 cctgaagatt aggctttata aaatctactt tatatgaaag ataaagtagc ttctcagatt
     2461 ttttacacga cacaggctct actatacatt gtatatcttt tttatacatg tattttaaaa
     2521 tattattaat cacatcgata tctatgtttg tatctataat aatgattttg cataaatcga
     2581 ttttatctaa attttcagtt atataattaa tatccactgt atcagatatc ctcatatccg
     2641 aaaaaccgga ttccatttca cccgtattat tatcaacaga aatatatatt ccggtagaat
     2701 atttctcact gatctttata cgggaaatat caactcctga agatgctgtt tccgaaataa
     2761 cctgttgacc ataccaatcc ttacctatgg ctcctaaaag tattacattt gctccgaaaa
     2821 gagcaagatt ctgagcaata ttccttccta ctccaccaca tgaaaaaaat attcttccgg
     2881 gattagaagt tcccttaata attggttcaa aggttctgat cttcatatct atatttattc
     2941 cgcctatgac acatatgctt tccaacatat tcacacctct ataacaatta tacagtatat
     3001 ataaaaaaag agtgccataa taacataatt atagcactca atactttccc ccgagcttaa
     3061 tatttttagg aaaaagagag agaggaaatt cacccattca gattatagca cttttaactt
     3121 ttattaagaa tatttaaaaa aagcgattat ctttctataa caaaaaataa acgtatataa
     3181 ttgtaattat aagagaaaaa catattctat tatgcataca atgaatttta aatttgttta
     3241 aggaggtaat aaatgtttag tgtagcccaa gctcaggagt atctaaacac aattttgaat
     3301 cctcaagact atgatgattt ctgttttaat ggagttcaga ttgaaggctt gtctgatgtt
     3361 tcaaaaattg cttatggtgt ttctcttaat gatgatttta ttgagaaaag tattgaacaa
     3421 ggtgccgaat tactgcttgt acatcatgga atatttggaa aagatttcat aaacttacat
     3481 ggtgtaaaaa agaaacgaat tgaaaaaatt atcaagagca atctcactct tatgagctat
     3541 catctgccgc tggatatgaa tattccttat ggaaacaaca tatcagtcct gaaaaagcta
     3601 aagttgaagt atctaaagac tattggcttt ggaatgatcg gcgtatatga gaagcctatt
     3661 ttctttaatg attttgagaa aaatttatat gagacttttc ctcggtcaga actacagatt
     3721 tacagaaata cagaaatgat ttcaactgtc ggaattgtaa ccggtgacgg aagtggagaa
     3781 attgagaagt atgaaggatt ggttgacact tttataactg gatcagaaaa agaatataca
     3841 cggaactggg taaaggaaag cggtatgaac tttataaatg ctggtcatta taatactgaa
     3901 attttcggta tttcaaatat tgctaatcta ttaaaaatta aatatggagc tgatataata
     3961 tttattgatg ttccaaataa tttgtaagta tgataaaaac aaatcgttca agtattgtat
     4021 atggaggtag aaatgaacgg acatgaactt ctttacgatg taaagtacta tgaagcagat
     4081 tataagaaaa gagtacttat aaccagtgta atgaattact ttcaggatct tgtagtgaaa
     4141 gacagcgaaa aaaagcaaat aggccttgat tttttaaaag agcgtgatat ggcatgggtt
     4201 attgccaaat gggatataga ggttaaaaaa tatccttcat ttaaagatac tttgttggta
     4261 aaaacaattc cggttgctct caagagcttt tatgcacacc gtcgttttga aatatggaat
     4321 gaaaattcag agcttgtgat atcagcaaat actttatgga ttcttataaa tataaaaact
     4381 aggaaacctg taaaaattga tgcactgttt tatgatgcat acggacttga ttacgagaat
     4441 actgataaac ttgattttga gaagctccaa attcctgaac aatttactgc tcaaaatgag
     4501 tttactatac gatacaccga tattgatacc aatgagcacg taaataatgt aaagtttgtg
     4561 gaatgggcta tgcagaccat acctttcgag gttataactt cgaaaacact tcacagaata
     4621 cagattgaat ataaaaaaga agccttgtac ggtgactctg tcttatcatg cagcaagatg
     4681 tttaaaaaag aagatctttt tgaatttcag catcttatat gcgattcttc tatggaaaaa
     4741 gagtttgcag ttctaaaaag cacatggaaa tgaaagtcta gtacattagt actagacttt
     4801 cattatattc tttgttttgt ttatagagta acggagtatg cttactataa gttcaattat
     4861 cactagaacg gtaataacta tgaaggcaac cttaagaccg atacttattc ctttgcttat
     4921 ctcttctgca atatccgtta ttctgaaatt ttccatttta atagtggcaa accatagcaa
     4981 tataaggtca aagaaaccct ttactatatc caaaagttca gtatttctgt agaacttcac
     5041 agcataaaca agtatgtcaa ataacccaac gaataagaaa gcaaaaaata ttgatttatc
     5101 agagatattg attacttgta atccgctttt actaagaaga actgttattg catcaattcc
     5161 gaaaaaaaca aacagtgtaa aaacagaaaa aaagattatt ttacttatca tttcaccaac
     5221 tttgggaggt tttactgcca aaaaggtaga aagatcttta tattcccaaa aagcggctat
     5281 attttttatt ttatacttgg tcagcataaa gaaaagaaaa catattattc caaaaagcat
     5341 gaaatatgtg ctaaagcatg ttcctataat ttccagtata gataagaaaa gattttctgg
     5401 cttaaaactg gtgaaactaa tcctaaaaac attagtaatc aggttaattc ctataataag
     5461 agcaaaaaat gtgtttgtga gcataaatat ataattttta tatgaagggt ctataagttc
     5521 ttgttcatca gagtattctt tagcaacctc tgcaggtgaa ccaaactcac ttaaaatttt
     5581 tgatatactt tcatctgtaa tctgtccgta ttttttttct gctttctcaa gcacgactgc
     5641 ttccagttcc tttataatat cttctttgtt atcagaagtg tttaggtaag ccttcacctg
     5701 attaagatac tcattaagtt tttcattcat tttctttcct cccatcgctt aatttttgta
     5761 atattagatt ctggatgctc caaattttca taatttcttc tctaagttct tttccatact
     5821 tagagatgct atagtacttt cttggtttat tatctaccac tttccactca cttataagaa
     5881 cttctttctt ctcgagtctc cttaaaagag gatataatgt attttcttca actttatatc
     5941 caattttttc aagactatca atgatatcgt atccataact tggcacatct agaaaagtta
     6001 gtatgatcaa ctgcagaaag cctctgttca tttcggacgt gatattcatg agttcacttt
     6061 gaatatccat tgtgtcacct cctatactgt cactaacatt atactatatg tcgcacagta
     6121 tagtgttaag taaaaaatac gtttgtgtta ttattatgtg aaattctttt ttgatttttc
     6181 tgaaaatcat ggcagcacat atggtcggtt tataaaaaac ttagtgtata atactttagg
     6241 gtggagaata tggatgaaaa acttcttgat tcatatatac aggaatttct tgcaaattta
     6301 aaatttataa aaagacgttc tgattcaact gtaaaagagt ataaaaaaat tcttacgtca
     6361 tataaaaagt accttatcaa gataggtata aataaagttg cttttttaac ttatcttcaa
     6421 gatatttcca atctttcgca aagaacagta agattaagga taatcgttat aagatcattt
     6481 cttaattatc tttatgaaag taattacata cagggtacaa attattggaa acaggcaaga
     6541 acttcattcc caccagaatc tcctaaagga cttaccgaaa aacaggttga gatattgttt
     6601 tcagtaatag atgattcata cgatgaaaaa ttttttactc tccttctaaa aactggtctc
     6661 agaatctccg aggctctttc acttaagaca gaaaatatac ttttttattc tgattacgca
     6721 gagatgattg ttataggaaa gggtaacaag gaaagaattc tgaaaatatc tctcagttat
     6781 gcaaagaatc ttgtaaaaga agctaaaaag atgatttttg aagtagacac ttccggtggc
     6841 gaaacttatg ttccaactca aagaaccatg caaagaaagt ttaagcgtta tacagaagta
     6901 gcaaatcagc tgattgattt tttacgttca aaaggagaaa atataaattt tatatctgct
     6961 actccacatt ccttgagaca tacctgcgca aaaaatcttc taaccgcagg aatgaatatt
     7021 gaagaagtac gttacatact tgggcattca aatatatcga ccaccggaat atatcttaga
     7081 acatcacagc aaaccaagat aatagcagag atttgattat aaaaaatcat caggatttta
     7141 ctcctgatga tttttttgag aagctaaaga agcaacccaa tctttattat tccataacgc
     7201 acatcctgaa gaagtttctt caagcttgtc atggtttatt cttatttcat gcaaaaaatc
     7261 agattttaat gcttcatcca aagatacttc atttatattg cagtcggaat aaggagaaaa
     7321 cggacatggt tctacatctc caaatgcact tatatgcaaa aaggctttgc cagaagaaag
     7381 acagccacca tactccttct catctccggg aaatgaaacg aaaattccat taaacttctt
     7441 tttcatttct tttgatatct ttgcaagatg atctcgttga tcctgatcaa ttacaagcga
     7501 atctgaacca ctggtaggaa catactcgat aaaaagaatc agtttacagc cttttttaag
     7561 caaagcatct atgtattcat ccgatgtaac ctgatcataa ttactttttg tcacagttat
     7621 tgacgcaccc caaataatat ggttcttatt aaacttttca gatagctctt ttaaagaaga
     7681 aaaaactcct ttgccacgtc tctgatcggt attccactgg tcaccttcaa tactcagcac
     7741 cggaataaga tttttctgag ttttgaaatt ttcaaatatc acttcatcca gtagagttcc
     7801 gtttgtaaat actgggaaaa ttatattttc attatttcgt gttatcttaa aaatatcttt
     7861 tcttaaaagc ggttctccac caattataaa tataagtgaa atacctattc tctttgcctg
     7921 agttacaata tctgatattt tttctgttga taattcttgt ttttccttcc ttttgtgcgc
     7981 ttttgcaaaa catcctatac aactaagatt acatctgtca gtaatgctta aaaaaagaaa
     8041 aggtggaaca tgtacccctt ctttttcttt tttattgcgg agagatgctg ctcttaattg
     8101 ataactgaga gctttcagtc cgaaaaaaat ataccttgga ttagtaatta ttactttaaa
     8161 tgattctttc atgaatttca ttacatcatt gttgaaagat actgcatcta atgattcttt
     8221 atccgtaaat atcacctcaa aggtttatac aaactttaaa gtttttccaa caaaaggaaa
     8281 agtccatata ttattgcttg cggagaagga ggacagccgg gaataaacaa gtctaccgga
     8341 agaatttcac taaggctttc cttaacggag tatccatctt tataaactcc attttcatat
     8401 gtgcacgttc cacatgcaat aacaattctg ggttcggcca ttgcataata agtctttaaa
     8461 agagcatgtt tcatatttat ggcaaccgga ccagtaacta ttataccgtc tgcatgacgt
     8521 ggagaggccg caaaagtaac tccataccgg gagatatcgt agtatgtatt gttaagagca
     8581 ctaagttccg acatacaggc attgcatgaa ccacaatcca cagatctcaa tacaaaagat
     8641 cttttataaa tactgaaaat tttctttctc atctcatctg gattgtcaat aagctgagaa
     8701 agtttgtaat catgagtagc tgttatggct tgatttgggc atgtagaaat acatcttcga
     8761 cagaaaacac atttgtttac atcatactcg atctttccat tttctatgct tattgccccc
     8821 gtaaaacaat tttctgcaca agaattacag taactgcaat tgctataatc aattttaaca
     8881 ggaccaaaag catatttatt gtctgaaata gggtttttaa cagtttcttt tttatttttc
     8941 aatctgtccc atgctattct catattatca cctgtcgttt tctgaataag acatattaaa
     9001 actcttatta cacaaaggaa aatccgggac tatctctttt cttactgcgt actccaatcc
     9061 tgaccagtta gagaatgaag gtgtcttaaa gtacactctg catggataat tattttttgc
     9121 acttcgaatg taaaggagaa gttctccttt taccgtctca accatactaa aaacttccgt
     9181 atgatcaggt atcttaatct gtggaacggt ttttacaata tctacattta tttcttcaaa
     9241 agattttaat ataaactcat atgactggaa aatttctttt acctttagtt gaaatctttc
     9301 ataaactcca cctaactcca tcgacggaat ctcatgaaca aacaacttat tgtaattatt
     9361 atacggaaaa atctttctaa cgtcatacct tattccgcag gctcttgcaa caactccggt
     9421 caatcctaaa gaaactcctt cctccatact tatacacccg gtacgctcta ctctgtcaag
     9481 aaaagagaca ctttgaactg tcatatccga tatgtattta aaacgcttaa ttatcttatc
     9541 aagcttattg ataactgtat ctttgtccac gatagcaaaa tcaattcccg aaccatttgg
     9601 aattattgca ctccggagaa atcgactgtt ggtcaactcc tcacatatct gatgaacaag
     9661 ttcagaaaga taagagaact tttttgaagg atatgaaaaa gctatatctg tacatattcc
     9721 tgctatatct tccagaaaat tgtaaattcg ttcaagttct actataactg tcctgaatgc
     9781 ttttatctct ggagatacag tttttcccat tatcttttct atggccagtg cataagccaa
     9841 tgcataagca gcagaagatt ctccactcac cctttccata ataacaggca gtaaattaaa
     9901 atttttggat tcgcatatct tttctatgcc acggtgttta tacattaacc ttatttcaag
     9961 attatctata ggttctccgc aaactgtaaa tctgaaatgt cctggttcaa ttatcccagc
    10021 atgcactggg cctacaggaa tctgaaagcc atcttcgcct ttcacagact caaaaatata
    10081 atttggaaca ttattcttct catttatctt ccttgatgaa tcaaaatcct ttctaagggg
    10141 aaactcgttt aaatcccaat tttcatgttt taccagacat cttgtatcag aagtacctat
    10201 aatttttaat ccataaagat ccgatatctc gcgttcatat atacctgccc ttggaatatc
    10261 ttgtgtaagt gaatcaatat atccatcggc attatagcgc aaaacaacca aaaatccatc
    10321 tttacgtgag gaaaatacca catttataac aaatacattt tcttcaaaac aaaactcaga
    10381 catgaaagac aatccaaaat catcggttag cctagctact atataccgta cctcaggctg
    10441 aattgtataa atataagctt cgttgctgtt ttttatctca atatcagtat taaaaacaac
    10501 ttttttgaac tctcttataa aatcttctat atgcatgtta tcatcctctt atcccaatac
    10561 tatcaaagtt gccttttcta atatcttatt caaaaaaggc agaaaataga gactggctaa
    10621 aactatcata attaaagaaa gcattaacgg aaatacattt gtttcttttt ctcttaaact
    10681 ttcttcatca ggcagagtat ctttatcttt gaaaaaaatc atacgagtaa aatgtgacaa
    10741 aaatgcacca aaaactacaa cagtaagaaa aacggttatg atagcaacaa ttgcatagtt
    10801 gctcgaaaaa cctgcattaa gtattttaag ctcgctgaaa aaagaagcaa acggtggcat
    10861 ccctgttaac aagcatacca ataattataa atacagagtt tataggtatt ttataaatca
    10921 taccattgat ttcagataga ttctttgtct tataactgac aataatatta ccggcaatta
    10981 aaaacaataa ggtttttgca aacgcatgaa taattgaatg aagaagagaa ccataaatac
    11041 cgtcaaaaaa cccaaatcct attcctatag aaatgatacc aatattttct atagaagagt
    11101 atgccaaaag acgtttaatg ttcttggctt taatcatact aaaagccgct accacaagag
    11161 ataacaaacc aaaaaccgta aaaaggaatc tcatatattt tagggcagat atattagaga
    11221 taattgtata aaaacgaatt ataacataca ttgccagatt tagcaaagcc ccagacatca
    11281 tagcgcttat cggtgccgga gcctgactgt gaccatccgg aagccatgtg tgcataggcg
    11341 ctaatccagc ttttgttcct aatcctataa atattagtgt aaatgcaatt ttaacaagat
    11401 ttatatcaag actgtcgctg atcgaaacca gataatccca tttaagtacg ttggcacttt
    11461 caacttttcc ggctgaatat ataaaaacaa ggataccaat caatcctaca gcgattccca
    11521 gtgaacaaag gattaaatac ttccaagcag cctcaagtga atgctttgaa ccgtcaaaac
    11581 taataagaaa agcagtcgaa agagtagttg cttcaagcca gatccacatg gaaatcatat
    11641 tattgcttat tgcaacaaaa atcattgaaa tagaaaatat actaaataaa ataaaatata
    11701 gagtggcctt ttttgaagaa attactcctt cttcaacctc agctgttatg tactttactg
    11761 agtatattga ggctatcaaa gctacaaaag taattataaa aagctggatg agtgaaagac
    11821 tgtctatata gtatgtacct gtgaaataac ttatgctgcc ataaatttgg atatcagctg
    11881 cgatttttaa cacaaccagc aaatttccta tactacttaa aacgcttata taagatttat
    11941 atcttacatt ttttataagt acaaaaaaaa gtactgaaat aaccgctata aagataaaat
    12001 atagtacttg catagtatcc ccctactctt taagatcgga aagataatcc gtatttgttg
    12061 tatcaaaagc agcattaatg ttccctataa ctatagccat tattataaaa agaattatag
    12121 cttctaccac tattgcaaac tcaataagaa aagaaagttt tataagggaa agttcaaaaa
    12181 gcataagtcc attttcaata accagaaatc caatcatttg ggaaagtgct tttcttcttc
    12241 ctataaggag cagagcgccg actattgaaa gagagatcgc ttcctttata tacaatatac
    12301 cgcttcccgc acttatatat gaggcaagag caattgaaat aatagtaaga gtgtatgacc
    12361 agaatgagtt taaaacaggt gaaagttcaa ctttagtttt gatctttctg accgaatgcg
    12421 ttagtattaa cggaacaagg attacttttg ccaaaagtgt catgatagaa ataacataat
    12481 aatgcttatc gtttgtactt agtgcggtca aaaaagaaat ggcaaatatg caaaacgctt
    12541 gcacagtata gcctgaaata agaagcttta ctcttctgac accactcata agaatagcag
    12601 aaatcagtat aaccgcaaga aaaaaatctg tcatttttat caccgcttat aagtatgata
    12661 ttacaagtga aattactgca agtgaaaatc cggccattat aatatcctga accttaaaaa
    12721 gcctgaactt ggccgtagaa atctctataa cagccaagga aaacaaaaat aagatcatct
    12781 tggtgaaata aaaaaacaaa gctgcaaaaa tattaatagg attcaaactt atagctattc
    12841 caactgggaa aaatatatta acaaaaaatg tagaaaacac cgctagtttt atagatgagg
    12901 caagttctag aaaaccaaga tcacaagagg aaatatccag taccattgcc tcatggacca
    12961 tcgtaagctc caaatgtgtc tcagggttat ccaccggtac tctcgtattt tcaactatca
    13021 gaaatattat aaaagcagat gcggcgatta tatgtcctat aaagtcattt ataacaatac
    13081 tgtttatata tgacatattc tccacactga atgttcctgt ttccataaat aaaaacataa
    13141 taagaacaag aaaaagaggt tcgctcatca ttgaaatgaa catctctctg ctgcttccca
    13201 tgcccccgaa ggtgctggcg cagtccaaac ctataagagc ggtcataaac tttacggcag
    13261 taaatgaaaa aaagactaga aaaactgaaa atccttgaac tttcggatac gcataaacta
    13321 caggaataaa aaaagttgta acaactgtaa atgcaagtat tgcactagga gcaaacgaag
    13381 taacagccga actgcttttt gatttaataa acgattttct gaaaagcttg cctatatcat
    13441 aatatacttg aaaaactgaa gggcctttat atcctctcag aaatgcctta cttttcttaa
    13501 gaactccgct gaataacgga gcaagaataa ttataagaaa tccatttaca aacatcatta
    13561 tatttttatc catcattttt atacctctat cccttaagaa ccaaaaagat cgtcaaagca
    13621 agcgttacgg caaaataaag aacataaagc tgaatatatc catgctgaat cttgtgaaaa
    13681 aatcttgata ctactccaaa agtccaaata aaaaaaggat aaacataatc ctgaagtaaa
    13741 tcattagcag aaatgcttat agaaatacct tttttttgct gaactttttt actataagaa
    13801 acagcatttt taaacaattt ggaagcaggc tgtgcataac cactggaggt atactgaaat
    13861 ttaggttcaa actctctgtg cccacatgcc catgtatctg agacctcttt ctttctgaaa
    13921 gtgccaagaa ggtatctgat aaaaacaaaa aaaataaaaa acataagtat aaagaaacca
    13981 aaaatcatta ttgaatcaaa gttttttgaa aaattttcag gaagtgtatc tccaaaaact
    14041 gatgagccga ttttcaatat tacatcaatt atataagaac tgaaaaaacc tgctgccaat
    14101 gagagtaatg atgacagaac cactgcaaaa cttata
//
LOCUS       DAOSHR010000033        14052 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_3602_length_14052_cov_6.741087, whole genome shotgun sequence.
ACCESSION   DAOSHR010000033 DAOSHR010000000
VERSION     DAOSHR010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 14052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14052
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3602_length_14052_cov_6.741087"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..954)
                     /locus_tag="PLU28_03070"
     CDS             complement(<1..954)
                     /locus_tag="PLU28_03070"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG34080.1"
                     /translation="MRCIRMSAKVKSIILYSLISGTSASIYSVVFNLFLRNNGLTNST
                     IGNFTSLELYGSAIIGTLLGVFGSSLGKKKIIVATACISGLIMLLRAFFPYPDMLIYT
                     AFLNGGMGVARNILLNVIILELTDNRTRAKAFGYNAGVSMGSGLIGNIIGGYFGDIFG
                     LKFTLILAGISFVLSTLVIINLSYADEPVKVKTQDLLVLKGLNCGQKRIVTGYVLTTA
                     FVGFGAGLFINFGNLIFFDLFKMSPAMIGAALSVAQLGTAIGSFYSHKLSEKFGAVQY
                     SAWMQFLVIPLVMMLGYIRDPYLFTACYALRYSLMNMTSPVT"
     gene            1081..1476
                     /locus_tag="PLU28_03075"
     CDS             1081..1476
                     /locus_tag="PLU28_03075"
                     /inference="COORDINATES: protein motif:HMM:NF022179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="late competence development ComFB family
                     protein"
                     /protein_id="HOG34081.1"
                     /translation="MIPKKLGIFNMMEFIVAEITEDLFLMNSFQICQCDKCKTDVIIM
                     ALNKLPARYVITEKGRIFTEIETCKSQYKVDVLKAVIESIETVRARPSHPPQKMKSSV
                     PDGIEDPNQVEAFLDELEDIINKKSTKNN"
     gene            1694..3022
                     /gene="ffh"
                     /locus_tag="PLU28_03080"
     CDS             1694..3022
                     /gene="ffh"
                     /locus_tag="PLU28_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOG34082.1"
                     /translation="MFENLQKKMASVFKTLSGKGRISESNIKEAVNSVKLSLLEADVN
                     YKVVKELVEKLKVESIGAKVMQSLSPDQEFIRIVRDELVEIMGGKEPVKLDLKRNPAY
                     IMLAGLQGSGKTTSAAKLAKMYKEKGRKPLLVAADTYRPAAIEQLVQLGRQIDVPVFS
                     GDRKNALEIVKQAKDFAIRNIHDIVIIDTAGRLHIDSDMMAEVRKIHDMVNPDETLMV
                     VDSMMGQDAVRAATEFNNQLELSGFIITKLDGDSRGGVIISIRYVTSKPIKLVGLGEK
                     LDNLEPFYAERYAGRVLGMGDVLTLIEKVEKEVQGEEAEKAAQKFLENKFDLEDFLQQ
                     LKQIKKLGPLSQILEMLPGNIPKENVDIAKGEKQMKKMEAIIQSMTPQERKNPKVLNF
                     SRKQRVAKGSGTSLQEINKVLQSYEEMKKMMKQFKSMGKKKFFGKMPFNM"
     gene            3054..3359
                     /gene="rpsP"
                     /locus_tag="PLU28_03085"
     CDS             3054..3359
                     /gene="rpsP"
                     /locus_tag="PLU28_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOG34083.1"
                     /translation="MVKIRLNRMGRRHQPFYRLVVVDSREKRSGQYIQSLGFYDPIND
                     KLPFKVDSEKAIEWLMKGAQPTDTARNILSRTGVMKKFDEMKHAQKVARKENKGAAN"
     gene            3359..3589
                     /locus_tag="PLU28_03090"
     CDS             3359..3589
                     /locus_tag="PLU28_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HOG34084.1"
                     /translation="MKEFLEEILKRIVKHPDDIAIVEFDDTSVKTFEIIVNNEDVGQI
                     IGKDGRTIKSINTILNAAKSSEGKKFVLKVIR"
     gene            3594..4160
                     /gene="rimM"
                     /locus_tag="PLU28_03095"
     CDS             3594..4160
                     /gene="rimM"
                     /locus_tag="PLU28_03095"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HOG34085.1"
                     /translation="MQRLDDLLKDRISVGKFSNTHTLSGELKLLPYTNATEVFSDLDE
                     VLLFNPEKKSFFFSKVLKVRRSNRLFILKLAGVEDVVSAEKLIGFEVFVDRSKMPHTG
                     ADEYYFYELIGCSVFDEEDFLLGTVEEVFQTGSNDVILVQKSISRFEKEELLIPLIKQ
                     NVISIDKLGKVIKITKPQYYEDDQKDED"
     gene            4132..4893
                     /gene="trmD"
                     /locus_tag="PLU28_03100"
     CDS             4132..4893
                     /gene="trmD"
                     /locus_tag="PLU28_03100"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOG34086.1"
                     /translation="MKMIRKMKISAVSIFPQIFSVLSDYGVTGKALEKKLIDFTAFDL
                     REFTSDKHKVTDRPGFGGGAGMVMLIEPFFNFYDYYMKTYSAKPYVVLMSPAGKTFTN
                     DIARELSQKDDLVFFCGRYEGIDERINSIVDMELSAGDFVMTGGELPSMLMIDAISRF
                     IPGVVGDENSVINDSFYDGLLDYPHYTKPAVFRNMTVPEVLRSGNHKEIDLYRKKQSF
                     INTILKRPDLFIKRELSAEDKKVLVEIITELIKDA"
     gene            4886..5467
                     /locus_tag="PLU28_03105"
     CDS             4886..5467
                     /locus_tag="PLU28_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOG34087.1"
                     /translation="MLDKIYVALIHYPILGRENQIISTAITNFDIHDIARSCRTYNVK
                     NFYIVCNLPAQKEIAKNVLKYWIEGFGKQYNPNRNDALSIARLKSYYEDVLQDIRSIE
                     GKDPKVVFTSAKPRENIVSYPQMSHIIRTEESPLLILFGTGWGMPEEIRETCSYELEP
                     IRGFSDFNHLSVRAAAAITLDRLIGEEVIKTIK"
     gene            5499..5846
                     /gene="rplS"
                     /locus_tag="PLU28_03110"
     CDS             5499..5846
                     /gene="rplS"
                     /locus_tag="PLU28_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOG34088.1"
                     /translation="MDALIRAIEKDFIRTDIPAFRPGDTVKVSVKVIEGGRERLQAYE
                     GIVIKIRGSGNGKTFTVRRMGADNIGIERVFPFNSPSIGKLEVTRKGKARRAKLYYIR
                     DIKGKVKIKERKD"
     gene            5852..6790
                     /gene="lepB"
                     /locus_tag="PLU28_03115"
     CDS             5852..6790
                     /gene="lepB"
                     /locus_tag="PLU28_03115"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOG34089.1"
                     /translation="MKSTTKDKIKKEALDWTYAAIYAIIFGTIIRLFVFETMMVPTES
                     MVPTVEVGDRMFIEKITYQYKEPQTGDVVVFWTPYVDQNALKMLGPWDKFMDFFAAKE
                     FTGHVKYVKRLVGKPGDTVKLVPVSQQRWNEISSGSVKSLPTWMKNLINQFGKAEYIT
                     KGVKDLVCMVQINGETPKEFEDLYYRKDAIFSSELFYEFMAFPAKYKQQILEAFQKTN
                     LRIFDLNTLRIYNQTLMYEDYYNSYLKNLDLSQYIYRDTDGLVAIKVPEGFYFFMGDN
                     SPNSFDSRFFGFVPVKNVIGTPFLRIWPLKRAGTVY"
     gene            complement(6823..6912)
                     /locus_tag="PLU28_03120"
     tRNA            complement(6823..6912)
                     /locus_tag="PLU28_03120"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6876..6878),aa:Ser,seq:tga)
     gene            6953..7596
                     /gene="rsmG"
                     /locus_tag="PLU28_03125"
                     /pseudo
     CDS             6953..7596
                     /gene="rsmG"
                     /locus_tag="PLU28_03125"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
     gene            complement(7712..8896)
                     /locus_tag="PLU28_03130"
     CDS             complement(7712..8896)
                     /locus_tag="PLU28_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HOG34090.1"
                     /translation="MELTHLIRKAARILKTPFLVLDLSYVEENYFRLKNSIENNEIFY
                     AVKANSHEKILEMLRDLGCSFDSASRGEINKLLGLGIDPKKISFGNTIKKEEDIRYAW
                     ENGIEYFAVDSEMEVEKIARNAPGAKVYGRIAMSSNDADWPLSGKFGTDVDHVIEILK
                     YANRKGLVPYGVSFHVGSQSYNKHKWKEAILSVSKVFEKLAEKGIYLKLVNLGGGIPV
                     KHVKPIPEVEEIGHVVNEAIHEYLGWVDGLRIFSEPGRSMVGNAGVMASKVVLRSRKG
                     TQDWVYLDVGVFHGLMETIENFRYEVVVDGKENHETVSMTLAGPSCDSVDTIYDEVEL
                     PVDIDYQDIVYFINTGAYTIEYASPHFNGITPPELYTVEELEVLVEKAEEELKEKENK
                     VN"
     gene            9233..10531
                     /locus_tag="PLU28_03135"
     CDS             9233..10531
                     /locus_tag="PLU28_03135"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOG34091.1"
                     /translation="MFKELGVKSFILKAIEEKGFKEPTPVQTLCIPALMEGKDVVVQA
                     RTGTGKTAAFAIPAIQKSFKREGIQVLILEPTRELTRQVDDEIMSLKKFIGLRIASVF
                     GGSSIINQIRNLERGVEIIVGTPGRVKDLIDRGILKLEKVKLFVLDEADEMLDMGFIE
                     DVEYIMSKLPVSVQKGFFSATFPQEVMNLVERNMNEPEHINTIPPEEANTFTQQFYAI
                     SVESKYGKLKELLNEDGKFMIFCRTRMEADDLFYKLKMDGFRVLELHGGHTQKMRNMS
                     IDTFRRWNNGILVATDVAARGLDIKGVTHTINYNIPEDIKKYVHRIGRTARAGHSGVA
                     ITIFEKRETRVIKELELITNDKMLPFESQDESILKQIRTDYYKAKQQNIRNERYQNSR
                     NSNNRMSDNKFTGRSTTNKSSGNYNKNHKPYKPNRVASVS"
     gene            10617..11417
                     /locus_tag="PLU28_03140"
     CDS             10617..11417
                     /locus_tag="PLU28_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34092.1"
                     /translation="MLKPIRKTAFYKYLKNEGHEISVFLRNDTLIRETLFSSNSRAYR
                     SLRIFNLVMSLIIFLPVVASLLDMNLPHFLGYLISALGIYLVWIFELITKSTLFEEIK
                     TFTIMQVSLHSVFGMYLQFYDKYTYFDDFLHITGGMWLSLMLFPIILAIELTFTSQKL
                     PTAIWKVNLSTFSLSVTMGTLWEVFEFISDLIFRDYPGYRLAQQDSLFDTMTDLIYDC
                     AGSILGIMLFWYLLRKSSKNRDIYNLLERIGKSLRMFMDRKKTRSENL"
     gene            complement(11414..12352)
                     /locus_tag="PLU28_03145"
     CDS             complement(11414..12352)
                     /locus_tag="PLU28_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase A"
                     /protein_id="HOG34093.1"
                     /translation="MDHRDIIQQVKQYTDTSIYRDSMKLQDIILTEFRSFLHQKGFLE
                     ILPVTVSNVTDPLNHEVFQAGFSYYQEQFTLTKSMIIHKQIAVLVHERIYCISPNVRL
                     ETSEKARSGKHLYDFVQLDMEMLKERREDVMDIMEEMISHTIKMLIETQQELLGKYHP
                     DLKVPSLPFKKISVKDALDIYGKDYEYELSIRTKEPFWIIDMPIKDREFYDRQNDYNP
                     DILLDFDLIYPKGFGEGISGGEREYEYEQILKRMKMKGTPEKNYEDYLKLAKENMLKP
                     SAGCGIGIERFTRYIMNLDHVEKTRLFSKVPGISSI"
     gene            complement(12608..13180)
                     /locus_tag="PLU28_03150"
     CDS             complement(12608..13180)
                     /locus_tag="PLU28_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HOG34094.1"
                     /translation="MTETTSILFAGVGGQGIVLTSKILSSALIQAGYDVKMSEVHGMA
                     QRGGSVTTQVRFGKKVFSPIIGKGEADILVAFEKIEAMRYVDYLKLKGKLIANDFKIP
                     SAPIFSAKVKYPRGIFADLEGKVYTKRVHAAEAAKELGNMKVQNIIILGVLAKVLGLN
                     NLDWKSSIKKEVKPEFFDLNLKAFEAGYYM"
     gene            complement(13183..>14052)
                     /locus_tag="PLU28_03155"
     CDS             complement(13183..>14052)
                     /locus_tag="PLU28_03155"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOG34095.1"
                     /translation="IPLTGELSPQVIRKAFLNEEPQYNKPTPERVVARPPILCAGCPH
                     RGIFFELSKRKDTVITGDIGCYTLGSADPLNAMDTVICMGAGISAAHGFAKAFAKTDR
                     KTKIFGVVGDSTFFHSGITSLIDIVYNKSNVTVIILDNRITGMTGHQENPGTGYTLMG
                     DKTDIIDIEQIVKAIGVKHVSVINPNQLKQVREVLDEYSALEEPSVIIAKYPCALKKL
                     RLEEKQSFNIKPSKYVVDPSKCKKCRMCLKIGCPAISFNEETGSSIDENQCIGCSVCS
                     QVCSFGAITKVGE"
BASE COUNT         4432 a         2541 c         2913 g         4166 t
ORIGIN      
        1 tgttactgga gaggtcatat tcatcaatga gtatcttagt gcataacatg ccgtaaacaa
       61 atagggatct cgtatatatc ctaacatcat aactagagga attactaaaa actgcatcca
      121 tgcagaatat tgtacagctc caaacttttc cgagagcttg tgtgagtaaa aagaaccgat
      181 agcggtaccg agctgtgcaa ccgaaagtgc cgctccaatc atagcaggac tcatcttgaa
      241 cagatcaaaa aatatcaagt tgccaaaatt aatgaataga cctgctccaa agccgacaaa
      301 agcagtcgtt agaacatatc cggtgactat tcgtttctgc ccgcagttca gtcctttcaa
      361 taccagaaga tcctgagttt ttactttgac cggttcatcc gcatatgaaa gatttattat
      421 gacaagggta gaaagtacaa aagatatgcc tgctaagatc agggtgaatt ttaaaccaaa
      481 tatatctccg aaatatccgc caattatatt gcctatcaga ccgcttccca tacttactcc
      541 ggcattgtac ccaaaagcct tggctcttgt cctgttgtct gtaagttcaa ggattatgac
      601 atttaaaagg atattccgcg ctactcccat tcctccgttt aagaaagctg tatagataag
      661 catgtcagga taagggaaaa aagccctcaa aagcattatc agacctgaaa tgcatgctgt
      721 agcaacaatt atcttctttt tgcccaagct gcttccgaag accccaagga gagttcctat
      781 tattgccgaa ccgtaaagtt caagagatgt gaagttcccg attgtagagt ttgtaagtcc
      841 gttattgcgt agaaaaagat tgaaaaccac gctatagatg gatgcgctgg ttcccgaaat
      901 aagtgagtac agtattatgc tttttacctt tgcgctcatt ctgatgcacc tcaaacgttt
      961 gtcttgctaa caaaatcatt atacatataa aaggttacgg aaatgcttaa aaaaaattat
     1021 cttacaaagt atatcggttt aaagttttat ggtataattc gaatgcggag gtgaataaag
     1081 ttgatcccta agaaactcgg aattttcaat atgatggagt ttattgtagc tgaaataaca
     1141 gaagacctct ttcttatgaa cagttttcag atatgtcaat gcgataagtg taagactgac
     1201 gttataataa tggctcttaa caagcttccc gccagatatg tgattacgga aaagggtcgc
     1261 atatttacag aaatagaaac atgtaagtcg cagtataagg ttgatgtgct taaggcagtc
     1321 attgaaagca tagagactgt aagggccagg ccgagtcatc cgcctcagaa gatgaaaagt
     1381 tctgttcctg acggtataga ggatccaaat caggttgaag cttttcttga tgagcttgag
     1441 gatataatta acaaaaaatc aacaaaaaac aattgagttc tttgttaatt gatttataaa
     1501 aaaagctttg aaatataaga ttaatggata ttagagaaaa taatgctttg aatcaattca
     1561 aagtatctta tttttttgtt aacgaaaaag ttaccaacct gtttgttctt tgtatactgt
     1621 gttatcttct tttcacccaa aaaaattaaa tttatagtat acttaacagg gattatttat
     1681 ggagggtaca acaatgtttg agaacctgca gaaaaaaatg gcgagtgtct ttaaaactct
     1741 ctccggcaaa ggaagaatat cagaatcaaa cataaaagag gccgttaatt cggtcaagct
     1801 ttcgttgctt gaagccgatg tcaattacaa ggtagtcaag gaactggttg aaaagcttaa
     1861 ggttgaatcc ataggtgcta aggtaatgca gagcctatca cctgaccagg agttcataag
     1921 aatagtcaga gatgaactgg ttgaaataat gggtggaaaa gaacctgtaa agttagatct
     1981 taaaagaaat cctgcttata taatgttggc agggcttcag ggaagcggta aaaccacaag
     2041 tgccgccaaa cttgccaaaa tgtataaaga aaaagggcgt aagcctcttc ttgtagctgc
     2101 cgacacgtac agaccagctg ctatagaaca gcttgttcag ttaggaaggc agatagatgt
     2161 tccagttttt tcgggggata gaaagaatgc cctggagata gtaaaacagg caaaagactt
     2221 tgcaataaga aatatacatg atattgttat tattgatacc gccggaagac tccatattga
     2281 cagcgatatg atggctgaag tgagaaagat tcatgatatg gttaatccag atgaaaccct
     2341 catggtagtt gattctatga tgggacagga tgccgtaaga gctgcaacgg agttcaataa
     2401 tcagttagag ctttcaggat ttataataac caagctcgac ggtgattcaa gaggcggagt
     2461 aataatctct ataaggtatg taacttccaa gcctataaag ctggtgggtc tgggtgaaaa
     2521 gctcgataat cttgagcctt tttatgctga aaggtatgcc ggcagggttc ttggaatggg
     2581 agacgttctt acgctcattg aaaaagtcga aaaagaggtt caaggagaag aagcggaaaa
     2641 agcggcgcag aagttccttg aaaataagtt tgaccttgaa gattttctcc agcagcttaa
     2701 gcaaataaaa aaacttggtc ctttaagtca gattcttgaa atgcttccgg gaaatattcc
     2761 aaaggagaat gttgatattg ccaagggcga aaaacagatg aagaagatgg aagctattat
     2821 ccagtctatg actccccagg agaggaaaaa cccgaaggta cttaattttt caaggaaaca
     2881 gagagttgcc aaaggcagtg gtacatcact tcaggaaatt aacaaggttc ttcagtctta
     2941 cgaggaaatg aaaaagatga tgaagcagtt caaaagtatg gggaaaaaga agttctttgg
     3001 gaaaatgcca tttaatatgt aatatttaaa atattcagga ggtgtttttt agcatggtaa
     3061 agataagatt gaacagaatg ggaagaaggc atcagccgtt ttacagatta gtagtggtcg
     3121 attcaagaga aaaaagaagc ggacagtata ttcagtcgct tggattctat gatccaataa
     3181 acgataagct tccgtttaaa gtagattcgg aaaaagctat tgaatggctc atgaagggtg
     3241 ctcagcctac tgatactgca agaaatatac tttcaagaac cggagttatg aagaaatttg
     3301 atgagatgaa gcatgcccag aaagttgcca gaaaagaaaa taagggagca gcaaattaat
     3361 gaaagagttt ttagaagaaa tactgaagag aattgtcaag catcctgatg atatagcgat
     3421 agttgagttt gacgatactt cagtgaagac ttttgaaatt attgtcaaca atgaagatgt
     3481 aggtcagatt ataggaaaag acggaagaac tataaaatcg atcaacacca tcctgaatgc
     3541 tgccaagtcc agcgaaggta agaagtttgt ccttaaggtc ataaggtgag cggatgcaaa
     3601 gacttgacga cctgctgaag gataggatat cggttggaaa gttttcaaat acccatactt
     3661 tgagcggaga acttaaactt ctgccatata caaatgcaac cgaagttttt tcggatcttg
     3721 atgaagttct tcttttcaac ccggaaaaga aaagtttttt cttttccaag gttctgaaag
     3781 taagaagatc caacagacta tttatactta agctcgcagg cgtcgaagat gttgtatcgg
     3841 cagaaaagct tatcggattt gaggtttttg tagacagaag caaaatgccg catacaggtg
     3901 ctgatgagta ctatttttat gaacttatcg gctgttcggt ttttgatgaa gaggacttcc
     3961 ttttaggaac ggttgaggaa gtttttcaga caggctccaa tgatgttata ctcgtccaaa
     4021 aaagcataag cagatttgaa aaagaagagc ttcttattcc cttgataaag cagaatgtga
     4081 taagtataga caagctaggc aaggtgataa aaataacgaa gccgcagtac tatgaagatg
     4141 atcagaaaga tgaagattag cgctgtcagc atttttccgc agatattttc tgttctttcg
     4201 gattatggag taacgggaaa agctctggaa aaaaagctta ttgattttac tgcgtttgat
     4261 ctgagagagt ttaccagtga taagcacaaa gttacggatc ggccgggttt tggcggtggg
     4321 gccggaatgg tgatgcttat agagccattc ttcaattttt atgattatta catgaaaacc
     4381 tattccgcaa aaccttatgt agtgcttatg tctccggctg gaaagacttt tacaaacgat
     4441 attgccagag agctttcgca aaaggatgat ctggtgtttt tctgcggcag gtatgaaggc
     4501 atagatgaac ggataaacag catagtcgac atggaacttt ccgcaggcga ttttgtgatg
     4561 acaggcggag aacttccttc gatgcttatg attgatgcca tatctagatt tattcctggg
     4621 gttgtcggag atgaaaacag cgttataaac gactcttttt acgatgggct gcttgattat
     4681 ccgcattata ccaagccagc cgttttcagg aacatgactg taccggaggt tctcaggagc
     4741 ggtaaccaca aagaaataga tttatacagg aaaaaacaga gctttatcaa cacgatactt
     4801 aaaagacctg atcttttcat aaaaagagaa ctttccgccg aggacaagaa agtgctggtc
     4861 gaaataatta cggagctgat taaagatgct tgacaaaatt tatgttgctc tgatccacta
     4921 tcctatactg ggcagggaaa accagataat atcgactgca ataactaatt ttgatataca
     4981 cgatattgca cgttcgtgca gaacatataa tgtaaagaat ttttatatag tatgtaatct
     5041 gccggctcag aaagaaatag cgaagaatgt tcttaagtac tggatagaag gttttggcaa
     5101 gcagtataac ccaaaccgca acgatgcgct ttccattgcc agattaaaaa gctattacga
     5161 ggatgttctt caggatatac gaagtattga aggaaaagat cctaaagttg tatttacttc
     5221 ggctaagcca agagaaaata tcgtttcata tccccaaatg tcacatatta tcagaacgga
     5281 agaatcaccc ttactgatac tttttggaac aggatgggga atgcccgaag aaatacgtga
     5341 aacatgcagc tatgaacttg aacctataag aggtttttct gattttaacc atctttcggt
     5401 gagagctgcc gcagccatta ctcttgacag gctgataggc gaagaagtaa ttaaaacaat
     5461 aaaataaatc aataaacttt gtacaggagg gaattaacat ggacgctttg ataagagcta
     5521 ttgagaaaga tttcataaga acggacattc ctgcattcag accaggcgat accgttaagg
     5581 tttcggtgaa ggttatagaa ggcggaaggg aaaggcttca ggcatacgag ggaatagtaa
     5641 taaagataag aggcagcggg aacggtaaaa ctttcacagt aagaagaatg ggtgctgaca
     5701 atatcggaat agaaagggtt tttccattca actccccttc tatcggaaag cttgaagtaa
     5761 caagaaaagg aaaggccaga agagccaagc tttattatat aagagacatt aagggaaagg
     5821 taaagataaa agaaagaaag gactgagttc cttgaaatct acgactaaag acaagataaa
     5881 gaaagaagca ttggactgga cttatgcggc tatctatgct ataatcttcg gaaccataat
     5941 aagacttttt gtctttgaaa caatgatggt tccgacggag tctatggttc ctacggtcga
     6001 ggtaggcgac agaatgttta tagaaaaaat aacatatcag tataaggagc cacagacagg
     6061 cgatgtagtg gttttctgga ctccttatgt ggatcagaat gctcttaaaa tgcttggacc
     6121 atgggataag tttatggatt tttttgcagc aaaggagttc acggggcatg taaagtatgt
     6181 gaaaaggctt gtaggcaaac ccggagatac agtgaagctt gttcccgttt cgcagcaaag
     6241 atggaacgag ataagcagcg gcagcgtgaa gagccttccc acatggatga aaaatcttat
     6301 aaaccagttc ggaaaagccg agtatataac caaaggcgtc aaggatcttg tgtgtatggt
     6361 tcagataaac ggagaaactc caaaggagtt tgaggatctc tactatcgga aggatgcgat
     6421 tttttcttct gaactgtttt atgagttcat ggcatttcct gccaagtata agcagcagat
     6481 tcttgaagct tttcagaaaa ctaacctgag aatatttgat cttaacacac tgagaatata
     6541 caaccagact ttaatgtatg aagattacta taactcttat cttaagaatc tggatctttc
     6601 tcagtacata tacagggata cagatggtct tgtagctatt aaggttccag aaggttttta
     6661 tttctttatg ggtgacaact ctccgaatag ctttgattcg aggttttttg ggtttgtgcc
     6721 tgtgaagaac gtaataggaa caccgtttct cagaatatgg ccgttaaaaa gggctggaac
     6781 ggtatactaa aaaaataaac cgccttaagg gcggtttttt tctggcggag aaggtgggac
     6841 tcgaacccac atggggctat taaccccatc ggttttcaag accgacgcct tagccaatta
     6901 gactacttct ccaaaaccac aattatttta ccataaagga tggtggttgt caatgtatga
     6961 taatgtggat tatgtaaata ttctatcaag gtaccggctg gattttgaag cagaagttat
     7021 tgacaaattg atagagtata accgccttat tgtagactat cctgtcaacc ttacggcata
     7081 taaggattat gcccgcgggt ttgaagcact tattatagat tctattatag ctgtcataaa
     7141 caccatgata atgaccgacg aggtcagcaa gatagtagat ataggaacag ggggaggtat
     7201 tccgggaata cccttgccat aatatttccg gagaaaaaat tttatctcgt ggacagtaca
     7261 gctaagaaga ctaatgctgt agcgcttttc tgtaaaacac tcaatcttga aaatgtcgag
     7321 gtttttaatg agagaataga agattttgcc cataaacata ggaatttttt tgattttgga
     7381 acatgcaggg ctttgggaag aacagacatt actcttgaat atcttgcctg catgataaaa
     7441 aatcaaggac ttatcggcct ttataagggt ccggcttact atgcccagga aaaaaagtat
     7501 gcagataagg caagcaatat gctggggctt aaggaagata taatctatag ttacagattt
     7561 tacgaaaagg atgaagaaaa aacaagattt atgtaacctt cagaaaaata gggaatactg
     7621 catcaaagta ccccagaaaa aacggagttc ctgcaaatga accgttggca ggagaatgag
     7681 agaaaataaa aaacagctct tgcgagctgt tttaatttac tttgttttct ttttctttaa
     7741 gttcttcttc ggccttttca acaagtacct caagttcctc aactgtgtag agttctggag
     7801 gagttatccc gttaaaatga ggtgatgcgt actctatggt atatgctccg gtatttatga
     7861 aataaacaat gtcctgataa tctatatcga caggaagctc cacttcatcg tatatagtat
     7921 caacgctgtc acaggatggt ccggcgagcg tcattgatac tgtttcgtga ttctcttttc
     7981 catctacgac aacctcgtat ctgaagtttt caatagtttc catgagaccg tggaaaactc
     8041 ctacgtcaag atatacccag tcctgtgttc cctttctgct tctcagaact acttttgaag
     8101 ccattactcc tgcatttcca accatggatc tgcctggttc ggagaatatc ctcaatccat
     8161 ctacccagcc aagatactca tggatagctt catttactac atggccgatc tcttcaacct
     8221 ccggaatggg ctttacatgc tttacaggta ttccgcctcc caggttaaca agcttaagat
     8281 aaataccttt ttctgcaagt ttttcaaaaa ctttgcttac actgaggata gcttctttcc
     8341 acttatgctt gttgtatgac tgcgatccta cgtggaaaga taccccatat ggcacaagtc
     8401 cctttctgtt ggcatactta agtatctcta tgacatgatc cacatctgtt ccgaacttac
     8461 cagaaagcgg ccagtcggca tcattggagc tcatagctat tcttccgtaa actttcgcac
     8521 cgggagcatt tctggcgatc ttttcgactt ccatttccga atcaactgca aaatactcta
     8581 ttccattttc ccaagcgtac cttatgtctt cttctttttt tattgtattt ccgaaactta
     8641 tcttcttcgg atctattcca agccctaaaa gcttatttat ttctcctctt gaagctgaat
     8701 caaatgaaca tcccaaatct cttaacattt caagtatctt ttcatgagaa ttggctttga
     8761 cagcgtaaaa aatctcgtta ttctcaatcg aattcttaag tctgaagtaa ttctcctcaa
     8821 cataagaaag atcaagtact aaaaaagggg ttttcaaaat tcttgctgct tttctgataa
     8881 ggtgtgtaag ttccacctca tatcatcctc ctttatggat atatattatt tttatctttt
     8941 atcggatact ggaatatatt aaataatttt aatgtaacaa caaggtacat gctatgtaaa
     9001 aaataatcga ttacaatgta aagatatcta cgtagaaccc ctgtttttat tacattttga
     9061 gtgcgatata taaatttggc tttcataggc tgatttccga taagccaagg gagttcatga
     9121 ttgaaaaaga agcggattct ttagatatta ttactaaaaa gaagtaaatt cttaataatc
     9181 ttcctctata atcaaatagt agtagatttt caagtttttc ggaggaaaaa gaatgtttaa
     9241 ggaacttgga gtaaaaagtt ttattttaaa agccattgag gagaaaggtt tcaaggaacc
     9301 gacaccggtt cagacccttt gcataccagc tttgatggaa ggaaaagacg tagttgttca
     9361 ggcaagaaca ggaacaggaa aaacagcggc atttgcaatc ccagctatac agaagtcttt
     9421 taaaagagaa gggatacagg tgcttatact ggaacctaca agagagttga ccagacaggt
     9481 agacgacgaa atcatgagct tgaaaaaatt cataggtctt cgtattgctt ctgtatttgg
     9541 agggtccagc attattaacc agataagaaa cttggaacgt ggtgtggaga ttattgttgg
     9601 tactcccgga agagttaaag accttatcga tagaggtatt ttaaaacttg aaaaggttaa
     9661 gctttttgtg cttgacgagg ctgatgagat gcttgacatg ggttttattg aagatgtcga
     9721 atatatcatg tcaaagcttc cggtcagtgt gcagaaaggc tttttttcgg ctactttccc
     9781 ccaggaggtt atgaacctgg ttgaaaggaa tatgaatgaa cccgagcata tcaatactat
     9841 cccgccggaa gaagcaaata cttttacaca gcagttttac gccatttccg ttgaatcaaa
     9901 gtacggaaaa ctcaaagagc tgctgaatga ggacggaaag ttcatgatat tctgcagaac
     9961 cagaatggag gcggacgatc ttttttacaa gcttaagatg gacggcttca gggttcttga
    10021 acttcacgga ggtcatactc agaagatgcg gaatatgtct attgacacat tcaggagatg
    10081 gaacaacgga attctcgtag caacagatgt ggcagccaga ggacttgaca tcaaaggcgt
    10141 tacacatact attaactaca acattcccga agatatcaag aagtatgtgc acagaatagg
    10201 aagaacggca agagctggtc acagcggagt tgccataacc atattcgaaa agagggaaac
    10261 tagggttata aaggaacttg agcttataac caacgacaag atgcttcctt ttgaaagtca
    10321 ggatgaaagt atcctcaaac agatacgtac cgattattac aaggcaaagc aacagaatat
    10381 taggaatgaa aggtatcaga actcaagaaa ctcaaataat cgcatgagcg ataataaatt
    10441 tactggaaga tctactacaa ataaatcgtc gggtaattat aacaaaaatc ataaacctta
    10501 caaacctaac agagttgcct ctgtatcata aaaaatgcag tccttttgga ctgcgttttt
    10561 tatatggtat aattcaaata ctgatgactt taattaaact ttttcaaggg ggaaacatgc
    10621 tcaaacctat cagaaaaaca gcattttata agtatctcaa aaacgaaggt catgagatat
    10681 ctgtttttct cagaaacgac actcttatcc gtgaaacgct tttttcttca aattcaagag
    10741 cttacagatc actgagaata tttaaccttg ttatgtccct gataatattt cttcctgtgg
    10801 tagcaagctt acttgatatg aaccttcctc attttttggg atacttgata tctgcacttg
    10861 gcatatatct ggtatggatt ttcgaactta taaccaaatc aacacttttt gaggaaataa
    10921 aaacctttac aataatgcag gtatctcttc atagtgtgtt tggcatgtat ctgcaattct
    10981 atgacaagta tacttatttt gatgattttt tgcatattac cggcggaatg tggctttctc
    11041 ttatgctttt tccgataata cttgccattg agcttacgtt tacttcacag aagctgccta
    11101 cagccatatg gaaggtaaat ctttcgactt tttcactttc tgtgacaatg gggactcttt
    11161 gggaagtatt tgagtttata tcagatctga ttttcaggga ttatccaggg tataggctgg
    11221 ctcagcagga cagcctgttt gataccatga ccgaccttat atatgactgt gccggtagta
    11281 tccttggcat aatgcttttt tggtatcttc tcagaaaatc aagcaaaaac agggatattt
    11341 acaatctgct tgaacgtatc ggtaaaagtc tacgtatgtt tatggatagg aaaaaaacta
    11401 ggtctgaaaa tctttaaata cttgatatgc ctggtacttt agaaaaaagc cttgtttttt
    11461 cgacgtgatc aagattcatt atatatctgg taaatctttc tattcctatg ccgcatcctg
    11521 cggaaggttt gagcatgttt tcctttgcca gcttaagata gtcctcatag tttttctccg
    11581 gtgtgccctt catcttcatt cgctttaata tctgctcgta ttcatactct ctttctcctc
    11641 cggatatgcc ttctccaaaa ccttttggat atatgaggtc aaagtctaaa agtatatcgg
    11701 gattataatc attttgcctg tcatagaact ccctgtcttt tatgggcatg tctatgatcc
    11761 agaaaggttc ctttgtcctt atggaaagct catattcata gtccttaccg tatatatcca
    11821 aggcatcttt tacggagatt tttttgaagg gcagagacgg aactttcaag tcaggatgat
    11881 atttgccaag cagttcctgt tgggtttcaa taagcatttt tatggtatgg gagatcattt
    11941 cctccataat atccattaca tcttctcttc tttcctttag catctccata tcaagctgca
    12001 caaaatcgta aagatgtttt ccgcttcttg ccttttcaga ggtttcaagt cgcacattag
    12061 gagaaataca gtatattctt tcatgtacaa gtacagctat ctgcttatgg attatcatag
    12121 atttggtaag ggtgaactgt tcctgataat aagagaaacc tgcctgaaat acttcatgat
    12181 tgagaggatc ggtaacgttt gaaactgtaa ccggaagtat ctcgaggaat cctttttgat
    12241 gcagaaagct tctgaactct gtcaggataa tgtcctgaag cttcattgaa tccctgtaaa
    12301 tactggtatc tgtatactgt tttacctgtt ggataatgtc tctgtgatcc atattttttc
    12361 ctccttttag aataaaaaaa accgacgttc gtatgaacgt cggctattgg atgtgcaaat
    12421 atacacagcg cttataaccg acgccgcgta ttattattat ttacatttat actgtataga
    12481 acaggtagtt cattcgattt tacgtattct acgaaagaga gacagagttt ttgcacatca
    12541 atcaccatta tcttcccatg gaagtattta ataaggatta tatcatattt ttagaatttt
    12601 gcaactatta catatagtat cctgcttcaa aagctttcag attgagatca aagaactcag
    12661 gctttacttc ttttttgatg cttgatttcc agtccagatt attaagtccc agtactttgg
    12721 caagaacgcc gagaattata atattctgta ctttcatatt tccgagctct ttggcggctt
    12781 cagcagcatg gacacgcttg gtgtagactt taccttcaag atcggcaaat attccacggg
    12841 gatacttaac ctttgccgaa aagattggag ctgaaggtat tttaaagtca ttggcaataa
    12901 gttttccttt taatttgaga taatcaacat atctcattgc ttctatcttt tcaaacgcaa
    12961 caagtatatc ggcttctccc ttgcctatta taggagaaaa aacctttttg ccgaatctta
    13021 cctgagtagt tacagatcct cctctttgag ccattccgtg tacttctgac atttttacat
    13081 cgtatccggc ctgtatcaat gctgaggaaa gtattttaga agtaagaact ataccttgtc
    13141 ctccgactcc cgcaaaaagt atgcttgtag tttctgtcat aatcactcac caacctttgt
    13201 tatagcgccg aagctgcaga cctgagaaca tacagaacag cctatgcact ggttttcatc
    13261 tatggatgag ccggtctcct cattgaaaga gatggcggga cagccgattt tcaaacacat
    13321 cctgcatttc ttgcatttgg aaggatcaac aacgtatttg gaaggcttta tattgaagct
    13381 ctgcttttct tccagacgga gcttcttcaa tgcacaagga tactttgcta ttattacaga
    13441 aggctcttcc aatgcggagt actcgtcaag aacttctctg acctgcttca actgattagg
    13501 attgattact gaaacatgtt ttacaccaat tgcttttaca atctgttcta tatcaattat
    13561 atccgtttta tcgcccataa gggtataacc tgttccgggg ttttcctgat gccctgtcat
    13621 gcctgttatt ctgttgtcca gaataatcac ggtaacatta ctcttattat agacaatatc
    13681 tataaggctt gtaattccgg aatggaaaaa tgtagaatct ccgacaactc cgaatatttt
    13741 tgtttttcta tcggttttgg cgaaagcttt ggcaaaaccg tgggctgcgc ttatgcctgc
    13801 gcccatgcat ataactgtat ccatagcgtt gagaggatct gccgagccca gagtgtagca
    13861 gcctatatct ccggttatga cggtatcctt tctcttgctg agttcaaaga atattcccct
    13921 gtgagggcag ccggcacata agatcggagg tcttgccaca actctttcag gagtgggttt
    13981 gttgtactgc ggctcctcgt tgaggaatgc ttttcttatt acctgtggag aaagctcgcc
    14041 ggtaagcggt at
//
LOCUS       DAOSHR010000034        13975 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_3624_length_13975_cov_5.809483, whole genome shotgun sequence.
ACCESSION   DAOSHR010000034 DAOSHR010000000
VERSION     DAOSHR010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 13975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13975
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3624_length_13975_cov_5.809483"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3474)
                     /gene="uvrA"
                     /locus_tag="PLU28_03160"
     CDS             complement(<1..3474)
                     /gene="uvrA"
                     /locus_tag="PLU28_03160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOG34096.1"
                     /translation="MMIKNDELQLKNIGIIFNSNGLTAVFLKKEYIPALLHLELFSHC
                     IIFSDSQQQDKYNLFKSSISSEVCRIVKIDGKRGIIYLDRIAAADQSPVWDVKPYFPC
                     EDRVQSPTDPFSSFGTFRAENNCEPQEKDISEINNIERTVRKKGTIINFQGEYFIQFD
                     NKCFFEYEKINQFSHIKILWWFDRFDKPEYRKVIQCNPPYENAPKTGIFASRSPVRPN
                     PIAMTTAKVLRTDPEKNRVYINYIDCFEKSTVVEVLPYIAVYDRIEAFTLPQWLEHWP
                     QWLDEGATSSIPDKSEMEPSLKDRIKKYIKADSVCYSQEKRYDSFSDGVFRDEGYITI
                     KGAKQNNLKNITVKIPKNKITLITGVSGSGKSSLAFDTLYAESQRRFMDSISTSGKMA
                     FEQFEKPDVEQITNLPPAIAIEQKTLGRNPRSTVGTITDIYDYLKLLYARVGTRHCPQ
                     CGKPFTQMTENQIINTLLSIQPHNHITISPFGHNDDQKHFIFSEQKDRLFEDDLAKCV
                     SLMLKKGKGALRVLINEEDFLFQTTSICYHCEKIFFELTPADFSFNNPQSMCPVCKGL
                     GVILQVDPELIVSDPSKSLLDGASNWWGNLRKHRSKPNANWMKGEVLALAEQMNVDLE
                     LPWKELPQEFKTQALYGSKGKEVRFVYENSNGRKGEIIRPVEGACNIINRLFRENNGE
                     TAQRLVSAFMNEKECTTCHGERLSPQGRMVVLAGKRFPQATSMKISELLKWLLELQIK
                     LDPSELEIAQTLLNEMTKKLKSLSNVGVSYLSLDRAVPTLSPGEAQRIRLASQLGNGL
                     SNLLYILDEPSMGLHAKDHKYLIQSIKELRDNGNTVVMVEHNTQMILNADYIIDMGPG
                     AGEHGGYVIAQGNLSQLEENCTSLTAKALKEIENEPRSCSNRRIPYGWLKIIGATHNN
                     LKNIDVEIPLGLITCVTGVSGSGKSSLISKTLFPMLNNHFNASQDTAGSCQNILGIEN
                     IDNVLNISQQPIGRTPRSNPATYTGLLDEIRELFAAIPAAKKLGYRSNKFSFNSKEGQ
                     CPECSGEGRKCIEMNFMPDVWIQCPQCKGKRFNQQVLEIFYNGKNIADILEMTVEEAL
                     TFFSTQKKISRILTTMYNVGLGYIKLGQSALTLSGGEAQRIKLSKELSRETSSRCLYI
                     LDEPTTGLHYED"
     gene            complement(3499..4332)
                     /locus_tag="PLU28_03165"
     CDS             complement(3499..4332)
                     /locus_tag="PLU28_03165"
                     /inference="COORDINATES: protein motif:HMM:NF018531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HOG34097.1"
                     /translation="MLEIPEAMTLSNQIREKLLGKKILSAKANSSVHKFTWYYNNDPE
                     KYSLVLKGKIITDALSFGGMLEVRLNGAKLLFTDGVSLKYLGNDEKHPLKHQLLIEFE
                     DKSALTATVQMYGGVWCFNDEDVFDNPYYATAKEKPSVFSDSFNQEYFMNIINMPLAE
                     KLSLKALLATEQRIPGLGNGVLQDILWTARINPRKKVCMLESDEKKKIFSSIKSVLKS
                     MYDLGGRDTEKDLYGNNGGYKTILSKNTASSPCPVCGQTIIKENFLGGSIYYCPKCQP
                     K"
     gene            4611..5600
                     /locus_tag="PLU28_03170"
     CDS             4611..5600
                     /locus_tag="PLU28_03170"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOG34098.1"
                     /translation="MMTTIKEVAKLAGVSVGTVSKVINNQDGIKENNRKKVLDAVKKL
                     NYEPNIHARTLSSGKTNLVSIVVPTVGFEFQKIFLKSLDDVLRKSNYDSVVFPLLSRD
                     RLARFSSESNFLFHTDALILASLSPKNLFRDGRVPTRKPYLIVDSHGESSNSVIIDNY
                     LGGHLAGKHISLEKNGKLFLIGGYEPDDAFSSGVFSERKRGFEDAIRERGIESKEIEF
                     KNIVLDWNQAFDFGARISRTEKKFSVFSVSDLVAWGFIEGCRTNGLYAGKDFHIIGYD
                     DLSFSQKIGLTTISQPISELGEIAAEKVVEKIKGAANIENIVLSPRYVERTSN"
     gene            5616..6833
                     /locus_tag="PLU28_03175"
     CDS             5616..6833
                     /locus_tag="PLU28_03175"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter substrate-binding
                     protein"
                     /protein_id="HOG34099.1"
                     /translation="MKKYLVVFVVIFSLLGFSKELNVWTCYGESTVEYKNFEAMGKMF
                     EKETGIKIKWIPITDLTDLDSKLRVSAPAGVGPDLLVTVPHDSIGKWVKQNILKPVDT
                     ILSEDLKNFTESSLMAVRYDGKAYGLPYTVESVGLVYNKKYVSQPPKDWNEIIQISEK
                     LKSQGINGIVFPAGEAYHMYCILRGFGGYIFKWENNQYNIQDIGMNNKGAYQAIAFLK
                     ELFDRGILSKVLMDSSSMHSFSTGAFEEGKAAFQINGPWVIPGASKMGIDCGVVPIPE
                     LPNGEMPKPFMGVQMIAFNNYSKNQADALKFARFITSHDNMLKFALDTNRVVARKSVI
                     DSQEIKKNPLISGWSQQAVYGEPMPNIPEMSVVWTAWKDSLPLMYSGKQDIQKTLDEL
                     VEVLHNKLKMMQE"
     gene            6891..7847
                     /locus_tag="PLU28_03180"
     CDS             6891..7847
                     /locus_tag="PLU28_03180"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOG34100.1"
                     /translation="MTYRAFSKKISLQKKEIYPKKQLKAASLFLFPTVFAITVSTVIP
                     SLYNIFISFTNYGLNHYFEYDFIGVENYKKIFNLTQNSPFFPVLLWTIAWMVLSTLLN
                     YFIGMFIAIFLNNPNLPERNIYRGLLIIPWALPGVIAIQMWHGMLGTEGVLNQFLGMF
                     GIEAVSWFNSVFWSRFWVIIVNAWISFPYFMTITYAALQSIPTDLYEAAEIDGAGKVY
                     KFRKITLPLLNKTLSPLIITQFAFQFNNFNLIFLLTRGGPRNSLGDYFGSTDILVSYT
                     FNIMRELQQYGLTAAYGVITFFITVLIIVFTTLCLKGLKEEF"
     gene            7847..8677
                     /locus_tag="PLU28_03185"
     CDS             7847..8677
                     /locus_tag="PLU28_03185"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOG34101.1"
                     /translation="MNRLKKKNIWFVRMLAILLVVVILFPFYYVVLTSFKPGSSFYSG
                     SVFPKQFTLDNYSSLINNSKFLLWVKNSTILGGFSALLTVVVTMFGGYAFSRLRFPGR
                     KYGILFLMVLQMFPTSVAMVAYFKMLQIAGLLNTLTGIILIMGLGNAAFGTWIIRNYL
                     NSIPTEIDEAAYIDGANYWQTFWKVLFPLMMPILATQFIMTFIGVYNEYMFSVILLFD
                     PSKYPVGVGIKGFLASNYSVNWTVFCAAAVLGSLPIIILFLLLQKNITNGLTKGAVKS
                     "
     gene            8674..10017
                     /locus_tag="PLU28_03190"
     CDS             8674..10017
                     /locus_tag="PLU28_03190"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOG34102.1"
                     /translation="MKGKVGYEIFVDRFYNFNNKGYTFIPWNIPVKAYDKQEYDFYGG
                     NLKGISKKIEYLKKLSVDFIYITPVFKANTNHRYDTLDFFGIDEKAGTENDLLELKSL
                     LKENSIELYMDIALNHTSRFNSMKENIDFYTGKNWANVENLPELNLESAELKNYLFRE
                     DSSVMKSYLRKGLTNWRFDCAYDLGYEILKEIKHNMDKVCENEVIGEVWSYPLEWCKV
                     IDGVMNYYFQQLIKMLLYGKIDGTTFTAAVAKTSEECGDKIFKSWNILSTHDTPRIKN
                     IFGDLWKIAVMLQFTVPGSPLVYYGEEIGMQGEGDPYCRQPMLWQEDLQGNEAYCFYL
                     KLIDEYKKAKGINSGRIKQLFSSDSEVVCFERTSDEIRDTRFVIANPTNKMISACIIT
                     ENSSLMNYSKIKDVFSETEFEIFSSCIDAVIPPKFAGVFKIKEERSSYNPYKRIT"
     gene            complement(10370..11080)
                     /locus_tag="PLU28_03195"
     CDS             complement(10370..11080)
                     /locus_tag="PLU28_03195"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOG34103.1"
                     /translation="MDNIFIKEDEVIENAENAIRKNIMGSTEGEALYKDLLYEYKKLL
                     KQTKSLVKMSDMMQLQLHDLSRELERMSNTDGLTGIYNRRFFNEIFHKEWYSAFRGKI
                     SIGILMIDIDNFKKYNDTYGHLQGDRCLKLISEIIQKSVKRPRDMLARYGGEEFVVLL
                     AETDIKGCRAVAEEIIKSIEAKDIKHAGSPYFPRVTVSVGIAVTVPDDQDSCEKLLNM
                     ADEALYRAKAAGRNCCME"
     gene            complement(11073..11471)
                     /locus_tag="PLU28_03200"
     CDS             complement(11073..11471)
                     /locus_tag="PLU28_03200"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HOG34104.1"
                     /translation="MNDLIIEKTQSTPYINFSHEHKKLFISGESFPENAAKFYAPILE
                     WIKQIINDPNTDSFTADFEIIYFNSSTSKVFMTIFDTFENAVKKGMQITVNWRCDSEN
                     ETAIECGEEFKEDLSTLPFNIVLVKGVENG"
     gene            complement(11468..12028)
                     /locus_tag="PLU28_03205"
     CDS             complement(11468..12028)
                     /locus_tag="PLU28_03205"
                     /inference="COORDINATES: protein motif:HMM:NF038262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SiaB family protein kinase"
                     /protein_id="HOG34105.1"
                     /translation="MLNLYELYKNAKTKNIIFYYSGPIAHASIEGVAATLRKNLQYEG
                     TDEITSNAVFSVFIEQVQNILNYSAEKFSDINSDDEDLRAGVVAIGYEEGFYCIYCGN
                     KVYNQDIPKIKENIDSVRNLDKEHLKALYKERRRMEPPPSSKGAGLGLIEMARKAVKP
                     IDYTFIPMDEEYSFFTIKVVTGREKI"
     gene            complement(12044..>13975)
                     /locus_tag="PLU28_03210"
     CDS             complement(12044..>13975)
                     /locus_tag="PLU28_03210"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOG34106.1"
                     /translation="KACDVFMRKISSIKRKYILYISLICVISLFILSCISYIFSYRIV
                     LSTLKEKLSAYSLRYSQEIDSWLTVQSQKLEWISHDMSINEEYYSNRSNVYKLLSEKY
                     RSSLGIVNDYYMSYTDKTLISGSDWVPQSDYDCTQRNWYTLASQSRKTVFTEPYVDAD
                     ERSGLRMVITISTPVYSEDRLSGVLAADITIDSLVKLVNSINPAKGGYAFLVDGNNAF
                     ITHPEKDFLPTREKHSYIDKVFNGGYTKLSEQIASRSYSITQIKDFDGIDKYFILSEI
                     KSSGWFLCISIPQTEISNNLNILLIGFTVFTTLSALISMVIIYFLISGMIRPVISLTQ
                     AVKEFGDKKMHVRSTVYSQDEIGELCNSFNKMADLIQDYSTTLENKVLERTKELNEKN
                     LKIQESIEYANMIQIAILPSDSEMKAFLGDYFVIWQPRDTVGGDFYWTKNFDDGFLVM
                     VGDCTGHGIPGALMTMAVNAIIDHITQDICHNDPARILTEMDRLVNQLLHRDRSNDTV
                     SDGLDIGIVFISEKKDILFCGARISLFTADNAQIREIKASNHTIGHEVTQREKKFANI
                     SVKHSSSMALYLMTDGIKDQIGGDKKLPFGKTNVIKILESVWDKPMSEQKNLIYMKYK
                     EYRNTEISRDDITMFGLRL"
BASE COUNT         4106 a         2542 c         2623 g         4704 t
ORIGIN      
        1 atcttcatag tgaaggcctg tcgtaggttc atctaagata tacagacaac ggctggatgt
       61 ttcacggcta agctcttttg atagttttat tctctgggcc tcgccaccgg aaagtgttaa
      121 agcactttgt ccaagtttta tatatcccaa tcctacatta tacatggttg taagaattct
      181 ggagattttt ttctgggtac taaagaaagt taatgcttct tctacagtca tttccagaat
      241 atcagcaatg tttttgccat tgtaaaagat ttcaagcacc tgttgattaa atctcttacc
      301 tttgcattga ggacattgaa tccatacgtc aggcataaag ttcatctcaa tacatttacg
      361 gccttcgcct gagcattccg ggcactgtcc ctctttactg ttgaagctga atttatttga
      421 tctatagcct aatttttttg cagcaggtat agcagcgaaa agttcacgta tctcatcaag
      481 cagaccggta tatgttgcag gattggaacg cggagttctg cctattggct gttgcgaaat
      541 gtttaggaca ttgtcaatgt tttcaattcc gagtatattt tgacaagatc ccgccgtgtc
      601 ctgtgaagca ttgaaatggt tgttcagcat tgggaaaagt gtttttgata taagactgct
      661 ttttcctgat ccgcttacac ccgttacaca tgtaataagt cccaaaggaa tttcaacatc
      721 aatgttttta aggttgttat gggttgcacc tattatttta agccatccat aaggtattct
      781 acgatttgaa catgatcggg gttcattttc tatttcctta agtgcttttg ctgtaagaga
      841 agtgcagttt tcctctaact gcgaaagatt tccctgagct atcacgtatc ctccgtgttc
      901 accggccccc ggacccatat ctattatata atcagcatta aggatcattt gggtgttatg
      961 ttctaccatg acaacagtat tgccattatc gcgcaactct tttattgact gtatgaggta
     1021 cttatggtct ttagcatgaa gtcccattga aggctcatcc agtatataaa gaagattgga
     1081 aagaccgttg ccaagctgag atgcaagacg gattctctgt gcttcgccag gcgatagggt
     1141 tggcacggct ctatcaagtg aaaggtacga aacgcccaca ttgctaaggc tcttaagttt
     1201 tttagtcatt tcattcaaaa gtgtctgtgc tatttctagt tccgatggat caagctttat
     1261 ttgaagttcg aggagccact ttagcagctc gctaattttc attgatgttg cctgtggaaa
     1321 tctttttccg gcaagaacca ccatccgtcc ttgtggagaa agcctctctc catgacatgt
     1381 ggtacattcc ttttcattca taaaggcgga aacaagtctt tgtgcggttt cgccgttgtt
     1441 ttccctgaat aatctattta taatgttgca ggctccttca acaggtctta tgatttcgcc
     1501 tttcctaccg tttgaatttt cataaacaaa ccgtacctct tttcccttag acccataaag
     1561 tgcttgagtt ttaaattcct gtggaagctc tttccatgga agttccaaat caacattcat
     1621 ctgttcggca agagcgagaa cctcgccttt catccagttg gcatttggtt tgcttctgtg
     1681 tttgcggaga ttgccccacc aatttgaagc accatccaac aatgattttg agggatcact
     1741 tactatcagt tccgggtcga cctgcagaat tactcccaat cctttgcata caggacacat
     1801 actttgcgga ttgttaaaac tgaagtctgc cggagtaagc tcaaagaata ttttttcaca
     1861 gtgataacat attgaggtgg tctgaaagag aaaatcttct tcatttatta atacccttaa
     1921 tgctcccttt ccttttttca gcatcagaga tacgcattta gccagatcgt cttcaaaaag
     1981 ccggtctttt tgctcggaaa agataaaatg tttttgatca tcattatgac caaatggact
     2041 tatagtaata tgattatgag gctggataga aagtagcgta tttattatct ggttctcagt
     2101 catttgggta aagggttttc cacactgagg gcagtgtctt gttccaactc gtgcataaag
     2161 aagtttaaga taatcgtata tgtcagttat ggttccgaca gttgatcttg gatttcttcc
     2221 aagagttttc tgttctatgg ctatagccgg agggagatta gttatttgct caacatccgg
     2281 tttttcaaac tgttcgaacg ccattttacc ggaggttgaa atactatcca taaatctacg
     2341 ctgactttct gcataaagag tgtcaaatgc gagacttgat tttccgcttc cgctcactcc
     2401 ggttataagt gtaattttat tttttggtat ctttaccgtt atgtttttaa ggttattttg
     2461 tttcgcacct ttaatagtta tgtagccttc atcgcggaaa accccgtcgg aaaagctatc
     2521 gtatcttttt tcctgtgagt aacagacaga atcagccttt atgtattttt tgatccggtc
     2581 ttttaatgaa ggttccattt cgcttttatc agggatgctg ctggtagctc cttcatccaa
     2641 ccactggggc cagtgttcca gccactgcgg tagagtgaaa gcttcaattc tgtcgtatac
     2701 tgcaatgtaa ggaagaactt caacaactgt acttttctca aaacaatcga tatagtttat
     2761 atatactcta tttttttccg gatctgttct taacactttt gctgtggtca tagcgatagg
     2821 gtttggtcta acgggcgaac gacttgcaaa aataccggtt tttggtgcat tctcatatgg
     2881 agggttgcac tgtataactt ttctgtactc cggcttgtca aatctatcaa accaccacaa
     2941 tatttttata tgcgaaaact gatttatttt ctcatactca aaaaagcatt tattatcaaa
     3001 ttgaataaaa tactctcctt gaaaatttat aattgttccc ttttttctga cagttctttc
     3061 aatattgtta atttcactga tgtccttttc ttgaggctcg cagttgtttt ccgctctgaa
     3121 ggttccaaag cttgaaaaag gatctgttgg agattggacg cgatcttcac agggaaagta
     3181 aggttttaca tcccataccg gtgactgatc tgctgctgct atccggtcaa gatagataat
     3241 ccctcttttt ccatcaatct ttactattct gcatacctct gagctgatac tgcttttaaa
     3301 taaattgtat ttatcttgtt gttggctgtc tgaaaaaatt atacagtgac tgaaaagctc
     3361 aagatgtaaa agagccggga tatactcttt ttttaaaaaa actgctgtta acccatttga
     3421 attaaatata atacctatat ttttaagttg tagttcatca tttttaatca tcataccacc
     3481 tcctggtgct attctcttct attttggctg acattttggg cagtagtaaa tgcttccgcc
     3541 aaggaaattt tcttttatta tggtctgtcc acataccgga catggtgatg atgctgtatt
     3601 tttactgaga atggttttat atccgccgtt attgccatat aggtcttttt cagtatctct
     3661 tccacctaga tcgtacatac tctttaaaac ggattttatt gatgaaaaaa tcttcttttt
     3721 ctcatcagat tcaagcatgc ataccttttt acgcggattg attcgtgctg tccacagtat
     3781 gtcctgtaaa acaccgtttc caagtcctgg tatacgctgt tctgttgcca aaagtgcttt
     3841 taggctaagt ttttcagcca gaggcatatt aataatattc atgaaatact cttggttaaa
     3901 gctatctgag aaaactgaag gtttttcctt cgctgtagcg tagtaagggt tatcaaaaac
     3961 atcttcgtcg ttaaagcacc atactccccc atacatctgt actgttgcgg ttagagcact
     4021 tttatcttca aactcaatca gaagctgatg ttttagagga tgtttctcgt catttccaag
     4081 atatttcagt gaaactccgt ctgtaaataa aagttttgcc ccatttaatc gcacttccag
     4141 cattccgcca aaggataacg catctgttat tattttacct ttaagaacca aagagtactt
     4201 ttcaggatca ttgttgtagt accatgtaaa tttatgcaca ctgctatttg cttttgccga
     4261 gagtattttt ttccccagga gtttttctct gatctgattg cttaaggtca tggcctctgg
     4321 tatttcaagc atagtcatcc cccttttgtt atataaggtt atactatatt ttagcatata
     4381 gttcagataa agattttgct ttaattgctc tgttttgtca accttaaagt acaagaaggc
     4441 attgtataat tatgtttttc tttttaattt caagaataat cataaaaaga aatgatttta
     4501 atagttaata gagtttattt tccttttttt gtctttgatt tattttttac agcaaggtat
     4561 tatataaaat gaaccgattc attatgaacc gattcattat ggaggtgaag atgatgacaa
     4621 ccataaaaga ggttgccaaa cttgcaggag taagtgtggg tactgtttct aaagttatta
     4681 acaatcagga cggtataaaa gaaaataaca ggaaaaaggt attggatgct gttaaaaaac
     4741 ttaattatga gcctaatata catgcgcgga cactttcaag cggcaaaacc aacttggtaa
     4801 gtatagttgt gccgactgta ggatttgaat ttcagaaaat ttttcttaaa tcgctggatg
     4861 atgtacttag aaaaagcaat tatgatagtg ttgtttttcc attactttcg cgcgacagac
     4921 tggcaagatt ttcaagtgag tcaaactttc ttttccacac cgatgcgctt attttagctt
     4981 ctctttcacc aaaaaatctt tttagggatg gaagggttcc tacacgtaaa ccttatctta
     5041 tagtagattc tcatggggaa agctcaaact ctgtcatcat agacaattac cttgggggac
     5101 atcttgccgg aaagcatatc agtcttgaaa aaaatgggaa attatttcta ataggaggat
     5161 atgagcctga tgatgctttt tcgagcggag ttttcagcga aaggaaaaga gggtttgaag
     5221 atgctataag agaaagaggt atagaaagta aggagataga gtttaaaaac attgttcttg
     5281 actggaatca agcctttgat ttcggagcaa ggatttcaag aactgaaaaa aagtttagtg
     5341 tattttccgt ttcagatctt gtggcatggg gattcattga aggatgcaga acaaatggat
     5401 tatatgctgg aaaagacttt cacataatag gatatgatga cctttctttt tctcagaaaa
     5461 ttggattaac aacaataagt cagcctatat cggagttggg ggaaattgct gcagaaaaag
     5521 tagtagaaaa gataaaaggt gcggcaaata tcgaaaatat tgtactttct ccgagatatg
     5581 ttgaacgaac ttcaaattaa aggggatgag aaaacatgaa aaagtacctt gtagtctttg
     5641 tcgtgatttt ttctttattg gggttttcaa aagaactgaa cgtatggacg tgttatgggg
     5701 aaagcacagt ggaatataaa aactttgaag ctatggggaa gatgtttgaa aaagaaacag
     5761 gtatcaaaat aaaatggatt cctataactg atttaacaga cctcgattct aagttacgag
     5821 tatcagctcc ggctggggtc ggaccagatc tcctggtgac agttccgcat gacagcatag
     5881 gaaaatgggt taaacagaac attctcaagc ctgtggacac aattttgtca gaagatctca
     5941 aaaattttac tgagtcatct ttaatggctg tcagatatga cggaaaagct tatggtttgc
     6001 cttatactgt agagagtgtc ggactggttt acaataaaaa gtatgtcagt cagcctccca
     6061 aggactggaa tgaaatcata caaatatcgg aaaaactcaa atcacaggga ataaatggaa
     6121 tagtatttcc tgccggagaa gcataccata tgtactgtat tttaagaggc tttggcggat
     6181 acatatttaa atgggagaat aatcaataca atatacagga tataggaatg aataataagg
     6241 gtgcatacca ggctattgcc tttttaaaag agcttttcga taggggaata ctttcaaagg
     6301 ttcttatgga ttcgtcttca atgcattcgt tttctacagg tgcttttgaa gaaggtaaag
     6361 ctgcatttca gatcaacgga ccgtgggtaa ttcccggagc ttcgaaaatg ggtatagact
     6421 gcggtgtagt tcctatcccg gaacttccca atggtgaaat gccaaagcct tttatgggag
     6481 tgcagatgat agcttttaat aattacagca agaatcaagc tgatgcttta aagttcgcaa
     6541 gatttattac ctcacatgat aacatgctca aatttgcatt agacaccaat agagttgtag
     6601 caagaaaaag cgttattgat tctcaggaaa taaagaaaaa tccacttatt tcgggatggt
     6661 cacagcaggc agtatacgga gaaccgatgc caaatattcc agagatgagc gttgtatgga
     6721 cagcatggaa agattctctt cctttgatgt actcaggtaa gcaggatata cagaagacgc
     6781 ttgacgagct tgtggaggtt ctccataata aacttaaaat gatgcaggaa taaaaggtca
     6841 ggcaatttgc ctgacccccc tttccataag tcagtaagga ggtcgcccta atgacttaca
     6901 gagccttttc aaagaaaatt agcctacaaa aaaaagaaat atatccgaaa aaacagctta
     6961 aagcagcttc tctatttctt tttccaactg tatttgcaat tacagtatca acagttattc
     7021 cttcgctata caatattttt atttctttta caaactacgg tcttaatcat tactttgaat
     7081 atgatttcat tggtgttgaa aattacaaga aaattttcaa tcttactcaa aatagtccgt
     7141 tttttcctgt actgctgtgg acaatagcct ggatggtact cagtacttta ttaaattact
     7201 ttataggtat gtttattgct atatttctca ataatccaaa tcttccggaa agaaatattt
     7261 atcgtggact acttattatt ccatgggcgc ttccgggggt aatagcaatt cagatgtggc
     7321 atggcatgct tggaacggaa ggggtactta atcaatttct tggtatgttc ggaatcgaag
     7381 cagtttcatg gtttaattcg gtcttttggt caagattttg ggtaattatt gttaatgcat
     7441 ggatttcatt tccttatttt atgactatta cgtatgcggc gcttcagagt attccgactg
     7501 atttgtatga agcagctgaa attgatgggg caggaaaagt atataaattt cgaaaaatca
     7561 cccttccttt gcttaacaag accctttcac ccttaattat aacgcagttt gcattccaat
     7621 tcaataattt caaccttata tttttgctta cacgtggcgg acctagaaat tccctcggag
     7681 attattttgg atctactgat attttggttt catatacttt taatataatg cgagagcttc
     7741 aacaatatgg tcttacagca gcatacggag ttatcacttt ttttataacc gtattgataa
     7801 tagtctttac caccctctgc cttaaggggc ttaaggagga gttctaatga acagattaaa
     7861 aaagaaaaat atttggtttg taagaatgtt ggctattctg ctggtagttg ttattctttt
     7921 tcctttttac tatgttgtgc ttacttcctt caagccgggc tcttcatttt attcaggctc
     7981 cgtatttcca aaacagttca ctctggataa ttatagttct ctaataaaca actctaagtt
     8041 tttgctatgg gttaagaact cgactattct tggaggattc tcggcattat tgacggtagt
     8101 tgtaaccatg tttggcggat atgctttcag caggctcaga tttcccggaa ggaagtatgg
     8161 aatacttttc ctcatggtac ttcagatgtt tccaacatct gttgctatgg ttgcgtattt
     8221 caaaatgctt cagatagcag gcctgcttaa tacgcttacc ggtataatcc ttattatggg
     8281 attgggaaac gcggcttttg gcacatggat tataaggaac tacttgaact ctatacccac
     8341 cgagattgat gaagcggcat atatagacgg tgcaaattac tggcagactt tctggaaggt
     8401 actttttcct cttatgatgc caattcttgc cacacagttt ataatgactt ttataggcgt
     8461 ttacaatgaa tatatgtttt cggttattct gctttttgat ccttcaaagt atcctgtagg
     8521 agttggtata aaaggtttcc ttgcgtcgaa ttattctgta aactggacag ttttctgtgc
     8581 agcagcagta cttggatcgt tgcctataat aattttattc cttctacttc agaaaaatat
     8641 tacaaacgga cttacaaaag gagctgtgaa atcttgaaag gtaaagtagg ctatgaaatt
     8701 tttgtcgata gattctataa ttttaataac aaggggtata cgtttatacc atggaacata
     8761 cctgttaaag catacgataa gcaagaatat gatttttacg gaggaaatct caaaggaatc
     8821 agtaagaaga ttgagtatct gaaaaaattg tcagtagatt ttatctatat tactcccgtg
     8881 ttcaaagcaa atacaaatca ccggtacgat acacttgatt tttttggtat tgatgaaaaa
     8941 gcaggaaccg aaaatgatct tctggagcta aaaagtcttc tcaaagaaaa cagtatagag
     9001 ctatacatgg atatagcact gaaccatacc agccgtttta acagcatgaa agaaaacatt
     9061 gatttttata ccggtaaaaa ttgggcgaat gttgaaaatc ttcctgagtt gaatttagaa
     9121 agtgcagagc ttaaaaacta tctttttaga gaggattctt ctgttatgaa gagttatctt
     9181 aggaaaggat taacaaactg gagatttgac tgtgcgtatg atttgggcta tgaaatttta
     9241 aaagagataa agcataatat ggacaaagtt tgtgaaaacg aagtaatagg tgaagtctgg
     9301 tcttaccctc ttgaatggtg caaagttatc gacggcgtaa tgaactatta ctttcaacag
     9361 ctcataaaaa tgctgcttta tggaaagata gatggaacaa catttaccgc tgctgttgca
     9421 aaaacatcgg aagaatgcgg agataaaatt tttaaaagct ggaatattct ttcgacgcac
     9481 gatactccta gaataaaaaa tatctttggt gatctgtgga aaattgctgt aatgcttcaa
     9541 tttacagttc caggcagtcc acttgtatac tatggagaag aaataggaat gcagggggaa
     9601 ggagatccat actgcaggca gccaatgttg tggcaggagg atcttcaagg gaatgaagct
     9661 tattgcttct acttaaaatt gattgatgag tataaaaagg caaagggtat caactctgga
     9721 aggattaaac agcttttttc ctctgatagt gaagtagtgt gttttgaaag gacaagcgat
     9781 gagatacggg atacgcgctt tgttatagct aatcctacaa ataaaatgat ttctgcttgt
     9841 attataacgg agaactcatc cttgatgaac tattcaaaaa ttaaggatgt attttcagag
     9901 acagagtttg aaatttttag ttcatgtata gatgcagtta ttccaccgaa gtttgcggga
     9961 gtattcaaga taaaggaaga aagatcaagc tataacccgt ataaaaggat aacataacaa
    10021 taaaagccgt tatcaaacgg tttttattgt tttttggcag cttcaatagt atgagcatac
    10081 atatgcttaa ctgggaagat agaaaactcg ccgatccttt gtaaaaggaa gaatgttttt
    10141 tctttgcgta gccactatct gcaatcattt aagtgtacgc tccttgagac ttcttggtag
    10201 attaaacgag acataaagtg catctcaaaa agtatttttc ttaagagata ttcccaagga
    10261 atacctttgt gaaatgatac ttatgaatat gctatactgt gtgaaaaacc gtttgaatcg
    10321 ataaaataat aattagggat actttttctg tactgttatt tttttaaaac tattccatac
    10381 agcagtttct tccagcagcc tttgcacggt ataatgcttc atcggccata ttaagaagtt
    10441 tttcacacga gtcctgatca tcagggacag tcacagctat tcctacagat accgtgacac
    10501 gcggaaaata cggagaaccc gcatgcttaa tatccttggc ttcaatactt tttataattt
    10561 cttctgcaac agcacgacat ccttttatat cagtttctgc aagcagtact acaaactctt
    10621 cgcctccata tcttgccagc atatctctcg gacgttttac agatttttgt ataatttcag
    10681 aaataagctt caagcatctg tcgccttgca gatgtccata ggtatcgttg tactttttaa
    10741 agttatcaat atctatcata agaattccga tagatatttt tcctctaaag gcgctgtacc
    10801 attctttatg aaatatttcg ttaaaaaaac gtctattata tattccagta agtccatctg
    10861 tgtttgacat cctttcaagc tcacgtgaaa ggtcatgaag ctgaagctgc atcatatctg
    10921 acatttttac aagacttttg gtctgtttca acaatttttt atattcatat agcagatctt
    10981 tataaagagc ctctccttca gtgcttccca taatattttt ccgaatagcg ttttctgcat
    11041 tctcgataac ttcatcttct ttgataaaga tattatccat tttccactcc tttcaccaaa
    11101 actatgttaa agggtagagt agaaagatct tctttaaact cctctccaca ctctattgct
    11161 gtttcatttt ctgaatcaca gcgccagttt acagtaattt gcattccttt tttcacagca
    11221 ttttcaaaag tatcgaagat agtcataaat acttttgaag tactgctgtt gaaatatata
    11281 atctcaaagt cggcagtgaa actgtcagta tttgggtcat ttattatctg ctttatccat
    11341 tcgagtatgg gagcataaaa tttggctgcg ttttcaggga aagattcacc gctgataaaa
    11401 agttttttgt gttcatgaga aaaatttata tatggtgtgg actgagtttt ttcaattata
    11461 aggtcgttca aatcttttcc ctccctgtta cgacttttat ggtgaaaaat gaatactctt
    11521 catccatagg aataaaagta taatcgatgg gttttacagc tttacgtgcc atctctataa
    11581 gtccaagacc tgcccctttg cttgatggtg gaggttccat tcttcttctc tctttataaa
    11641 gtgctttaag atgttctttg tcgagattgc gaacactgtc aatattttct tttatctttg
    11701 gaatgtcttg attatatacc ttgtttccac agtagataca gtaaaatcct tcttcatatc
    11761 ctattgccac gacacctgca cgcagatctt cgtcatcact gttgatatca gagaattttt
    11821 cggccgaata attcagaata ttctgcactt gctcaataaa aactgaaaaa actgcatttg
    11881 aagttatttc atcagtccct tcatactgta ggttcttgcg cagggtagcg gctactcctt
    11941 ctatactggc atgtgcaata ggtccactgt aataaaaaat tatgttcttg gtcttggcat
    12001 ttttataaag ctcatatagg ttgagcaaaa gttttccctc cttttataga cgtaatccga
    12061 acattgtaat atcgtccctg cttatttcag tattacggta ctctttgtat ttcatatata
    12121 taagattttt ctgttcactc ataggcttat cccatacaga ttcaagaatt tttattacgt
    12181 ttgttttacc aaaaggaagt tttttatctc cgcctatctg atcctttata ccatcggtca
    12241 tcagatacag agccatagaa ctgctgtgct ttacagagat attggcaaac tttttttctc
    12301 tttgcgtaac ctcatgtcct atagtatgat ttgaggcttt aatttccctt atttgagcat
    12361 tatcagcagt aaaaagagat atccgcgctc cacaaaacag tatgtccttt ttttccgaaa
    12421 taaaaacaat tcctatgtca agaccgtcac tcacagtatc gttgcttctg tccctgtgta
    12481 aaagctgatt gacaagcctg tccatttcag ttaaaatccg tgccggatca ttatggcata
    12541 tatcctgtgt aatatgatcg attatagcgt ttacagccat tgtcataagg gcacccggta
    12601 ttccgtgtcc ggtacagtcg cctaccatga caagaaaccc gtcatcaaaa ttttttgtcc
    12661 aataaaaatc tccgcctaca gtgtctcttg gctgccatat cacaaaataa tctcccagaa
    12721 aagctttcat ctctgaatca gaaggaagta tggctatctg tatcatattg gcatattcta
    12781 tgctttcctg aattttaaga tttttttcat taagttcctt tgttctctct aaaactttgt
    12841 tttcaagagt tgtactataa tcctgtataa gatctgccat tttattgaag ctgttgcaaa
    12901 gctctccaat ctcgtcctga gagtacacgg tgcttcttac gtgcattttt ttgtctccaa
    12961 actcttttac tgcttgggta agacttatca caggccttat cataccgctt ataagaaagt
    13021 atattatcac catgcttata agtgccgaca aggtagtaaa aacagtaaat ccgatcaaaa
    13081 gtatgttaag attatttgaa atttcagttt ggggaattga gatacacaga aaccaccccg
    13141 aacttttaat ttctgaaagg atgaaatatt tgtcaatacc gtcaaaatcc tttatttggg
    13201 taatactata acttctagat gcaatttgtt ctgaaagttt tgtatagccg ccgttaaaaa
    13261 ccttatcaat ataagagtgt ttttcgcggg ttggaagaaa atctttttct ggatgagtta
    13321 tgaaagcgtt gtttccgtca acaaggaagg cataacctcc cttggccgga tttatgctgt
    13381 ttacaagctt tactaggcta tctatagtaa tatctgcagc caacactccg gataatctgt
    13441 cctctgagta aacaggtgta gatatagtaa ttaccattct taaaccggat ctttcatccg
    13501 catcaacgta cggttcggtg aaaacagttt ttcggctctg agacgcaaga gtataccaat
    13561 ttctttgtgt gcagtcgtaa tctgactgag gaacccagtc agaaccggaa attaatgttt
    13621 tatccgtata gctcatataa taatcattta ctatgcctaa cgacgacctg tacttttcag
    13681 acagtagttt ataaacattg gatctgtttg aatagtactc ttcattgatg ctcatgtcat
    13741 gacttatcca ttcaagtttc tgtgattgaa cagtcagcca tgaatctatc tcttgtgagt
    13801 accttaaaga gtaagcggat aatttttcct taagtgttga aagaactatt ctgtaagaaa
    13861 aaatatagct aatgcaggaa agtataaaaa gacttatcac acatatcaaa gatatgtaga
    13921 gaatgtactt cctttttatg cttgaaattt ttctcataaa aacatcacat gcctt
//
LOCUS       DAOSHR010000035        13925 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_3636_length_13925_cov_6.092141, whole genome shotgun sequence.
ACCESSION   DAOSHR010000035 DAOSHR010000000
VERSION     DAOSHR010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 13925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13925
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3636_length_13925_cov_6.092141"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            58..1197
                     /locus_tag="PLU28_03215"
     CDS             58..1197
                     /locus_tag="PLU28_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOG34107.1"
                     /translation="MDILIKKGFICPVVSKAFTGDILISGGKIKKISESITAKDVQVI
                     DAKGKFVLPGFIDAHSHIGLFEEGVGQYYQDGNEATDPITPQVRAIDAFNPQDSAIKK
                     ALSGGVTTTMILPGSANAVGGQGAIIKFNSQIVDQMIIKEPAGLKMALGENPKRFYGE
                     SMKRTPGTRLGTAAMIREYFVKTQSYSEKKQKGDYEKDLRYEIGEMVLKRKIPARIHA
                     HRMDDILTAVRIANEFEFDFVIEHATEAYKIIEYLKARNVKMVLGPLFSFATKLELKD
                     KTFEAVSIAVKNNVPVALMCDHPVVPMEYTIMQLGTALKYGVSEEELLKLVTINPARI
                     LGIDDSTGSLQKDKDADIAIWSGNPFEMKSCVEQVLIQGECVFSR"
     gene            1211..2710
                     /locus_tag="PLU28_03220"
     CDS             1211..2710
                     /locus_tag="PLU28_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOG34108.1"
                     /translation="MKYAKIKTACINGFDVREIIVELDINSRSTIQTFKIVGMAATTV
                     LESEKRAMSCIRNCGFTVPNGSITANLSPSSLRKEGTHFDLPVAVALLQASSQIKSAD
                     GDFYFFGELGLNGEIRPVNGIALFLISLKQSNPQAKFIIPKENENESYFIDPNDVYVI
                     SSLNDICRIFSEERESFLPHVGSCAGKTFDMDFSEIKGQLFIRRAVEIACSGFHNILL
                     KGSPGSGKTMIARRIPTILPPMTTEEIIESTKIYSVCGYINKIINERPFRSPHHTASS
                     ASVIGGGNIPRPGEISLAHNGVLFLDEFPEFRNDVIQSLRQPMEDGMVTVTRARSVAE
                     FPARFMLVAAQNPCPCGFFGDKEKECTCSINQISSYNKKISGPITDRIDIKIDVPRVE
                     ISDLLGKDDGESSEKIKERVMKTSSIQIKRQGKFNGQLTGREVKEYAILSDKAEEFLK
                     TAAVKLKLTARSVTKIIRVARTVADTYESEQIQTVHIGEALNYRNVEIN"
     gene            2853..4844
                     /locus_tag="PLU28_03225"
     CDS             2853..4844
                     /locus_tag="PLU28_03225"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOG34109.1"
                     /translation="MKSIRSRLMFWLITLFTAILLIAGVILYAQTQSTVVPTVEKMAE
                     QIVIARADEIGEWLNGILIEVSAQSDRNVVKTMSWTNMQESLLRYINDKNKIFESLWV
                     CDLKGDFTSTSGRTGNIYSAEYFEAIAKKNLNFFITNVMYSQETKEPIFVVTKSILND
                     KKELIGVMGANVLINTVSEIVGSINIDNSGYGYIVDGTGMVLAHPKKEVVLNMNLLKG
                     TQLGYKGLEDIGQQMIEGKAGMGIASLTDGTKNVTFFSPVPYAPNWALAVAVPQTYLY
                     RTSNALAMTLILLILVLIVISIGLSVFIGGTIANPIKKLMKKIDTFGSGDLTVDFELK
                     QKDEVGKIASSLKSMAASLKQAMFSIQEASGQMQSSSSDLAAIAEETSATSEELSSQV
                     ESISSNYSQTNQSITEVKEAVVQVAKNSQEVTQSSLNLLQSTRIVKGSVETGEQSIGS
                     IAALVQESSQKADETKKLLDRLKDNTDNVGKIVDTINSITEQTNLLALNAAIEAARAG
                     DAGRGFAVVADEIRKLAEESKKATANIANILSETREYAQDSKESFDEIAGNIHNISNE
                     TLKVKKEFNGILGQISQISSMTEQVSKNSQSQSSASQEIASSVENSAKMADDINFQLN
                     STVTAISQQALAAQQIGANSEELSALAETLSQQVKKFKT"
     gene            4841..7315
                     /locus_tag="PLU28_03230"
     CDS             4841..7315
                     /locus_tag="PLU28_03230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG34110.1"
                     /translation="MKDGSEQPGNYFIKILINIFLVSIFLTINLFIIFIESSHIKELT
                     FSLGESVSRDISLRMNNSFSSLIALGYTFETEGELTQNRFEALSRYFIREFPNVSFLQ
                     HKNAQTVTDMVYPLWGNEATIGQSLMDNEQALKSLEKAKQTRTLTVNDPYMLKQGYLG
                     MIARYPVFVDDEFAGLYVCGINLDEFLSFLSKKEIFGSYDYSFFDSTGKVFFESSTEN
                     KKNFSQEFKIPIADSYWILEIYPKNDYLMNFILYSIILWGLYLLVSFFVGSLEHRLNR
                     KDQLIKELKDLKIKLTEEAYEKETIAADLRKGQELYRNMFYSNTSIMFLIDPQTLYIY
                     DANPAAVKFYGYPLEEFKKLKISQINVLGDEVVHKSVNSVIDSNFSFFSFKHKLSDGT
                     IRDVEVHASKVQINDNAYIYSIIHDVTSRIKMQEKLNLIARLQSIVVDIATKVINLSS
                     FYIEDTVNSALEELGKLLEAERIVVFFNEDKSSYKNWEWKSPESSSKDVCSTYSDYED
                     ILKQLDKNDKDFLFIRSSSCSSVFIPMNTDKKISGFIAMSGNSKEYNFDPQVLKAVKL
                     IAQTIASGIKRKEYENIILSKKEEAEKASYAKTAFLSRVSHELRTPMNGIIMGCQIAQ
                     DCDSLQEVKEYTELIKKSADSLMSLIEDLLDLSKIEAGDVDLQILDFNVENVLRYLEE
                     EYRSRAKDKGLDFFFSVDEKMPLKIYSDPGKIMRILKKLLDNSIKFTEKGFISLSVEN
                     VHREREYVRFKVSDSGVGIEEEKYGFIFQQLVQLEEHSARKYFGTGSGLFIAKKLVKL
                     LGGEIFFESDFGKGSTFYVEIPYKPI"
     gene            7329..7868
                     /locus_tag="PLU28_03235"
     CDS             7329..7868
                     /locus_tag="PLU28_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34111.1"
                     /translation="MMRVEGTNGAADWISRYKSLSKGKDGTEAKSSEKVKNRITQNSS
                     EQENDSDIDIRTQKKLGIKECETCANRKYQDGSTDGGVSFKAPAHISPQAAPAAVRSH
                     EQEHVANEQAKAQSSGKEVISQYVRIFTAVCPECGRVYVSGGETVTTTMDKKNSGFEY
                     DSSLKYSQKAQGNFFDRSA"
     gene            8029..10749
                     /locus_tag="PLU28_03240"
     CDS             8029..10749
                     /locus_tag="PLU28_03240"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOG34112.1"
                     /translation="MRSKFLRIAVVGIFSAVTIFFALRLLNIKISDDIMTYMSEENKE
                     ISFYREVSEKFDIDNIMMISVEMNSVYDELNEVESIRKELYSLKDVKKVLSITNSLIV
                     SSDEYEIKSGSLSTILDLSSYNVAEVEKILETDSSLKDTFVSKDGKNVLYILNLDDSK
                     KVDIGLLLEEIEKTLSTRTYYLTGLPLANYEIAQTAFSDVVRILPFAILGIIAILFTV
                     FRSFAGVMLPIVAVIISNAVAMGLVTIFSESFSTVTIMMPIILVGIGVDNTVYLISRY
                     NEEYRHDEDKNAAVTRARKKILTPVLMCTLTTVAGFMSLITARLIPVTQLGFYTSAGI
                     IVSFLVAVVFMPALILIVKPRAKQSSDKENRKSLWAAITLKVIKHKVLVSTVMVIIFL
                     CFILIIPGIKTESNLETYLGSSALTIKGSNFINEKFGGDKFINVYMKSGIEEGYKDFY
                     YNRVQRDIQKYVLTIDSHFISRGLSTFTSSVVKSFSGTEHIPGNSSTMNQVFLIIENT
                     PELSKFLLKPQSESVIDVRISSNNDTQVQAILEKLNGFIEKYVFKSYDKALLDINNPK
                     ALKEYEKSLQSFFVSRNISHAAEMFSMLAENKKKSLSQVVTEYPENELYGKYQSFCEN
                     FSLQADEKSIFLKNIKNEEYTETFTNFLSFNEQTLRSRVFYEELKKFFPAVQDGDLAE
                     FSEYVNDREYVTDTGSIPLYLKVTGNPIIRTFINSELLYGQMTSLFFSYIFILLMFII
                     SSRSVRTGLIASLPLLFTTVANFGFIRLFNFSLNAGTITIASIVIGLAIDYSVHYVSR
                     FREEFLKTMDICKSLEITSKTVLRAISTGAFTTLLGFFPLGFGNLGIMKQFGIISGIS
                     ILISLLSTVVILPIVFSSIKKETLLKILQKRKADKNEKKSFFLSVFNRYR"
     gene            10703..11476
                     /locus_tag="PLU28_03245"
     CDS             10703..11476
                     /locus_tag="PLU28_03245"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HOG34113.1"
                     /translation="MKKSLFFFLFLTVTASIFGLTAQDVLDKVKDSQNNFETQKAVYS
                     IILSDSNKTQKTRKIDIYILISSDKGEKLPISLLRVTEPKDLQNTTVLNLSKTEQYIY
                     LPGFKQVKKVSGSSKNESFLDTDINYSDMTLLFSSTIEQEHTSSISSEDEKSYTIDIK
                     LAAQDSDYSKMTVGVDKKDFVIKYAEFFDKKEQIVKKMEIMDYESSGEYITYKKVLIT
                     SLENKHSTLLELVSSEYGIPITKSFFNKLSMTNPVLVYR"
     gene            11488..13089
                     /locus_tag="PLU28_03250"
     CDS             11488..13089
                     /locus_tag="PLU28_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34114.1"
                     /translation="MKKVMVFICTIWAAGVIMGFSGKSIYTLGYNYQVDFVDIYQLLN
                     TDIEFESQNESSYFNLCINITALNGLSPESTNVYIDSYLLGYKDIKAFFPGLDEILVN
                     SIFYGEIKELYYDQYFSGNTLRVGRFYPNKASANLYSPSNVMQASNFLGYLNENTLTG
                     IDGLVLQLFFEQMSAECYFTPVTKYDFPNLTAYEKTQSIYDLLLEKSGSESDYLLLAY
                     KYKDRNVLRLDDMNTGLKIDFSIFDFSVRPGYFRNNYHFDIPELVEVDFIKNDIMLNL
                     HINDITIDLSKYEFPPEIKDNPIFAELLKQKIIFDTDYSTSMTINTSAATSTIINPWA
                     DVITADIQYSSKLIEGLTWYFEAGLYLPQQITTKGEYSKIKRQDTIDITFEGTTNVFY
                     SYTKQQNKEAYVPNVFSESLLGNRYVKYIAGFEYLTGDFSFGLEFFNGMNQEHFSMTP
                     GIDGFFNYSTKIFDINLFGLVTFPEIHGKIDIGYTTVAKAAYTGMDQTRIGLKSTYSY
                     SENEKNIIYTSKMLNSVEFFIEKYF"
     gene            13103..13552
                     /locus_tag="PLU28_03255"
     CDS             13103..13552
                     /locus_tag="PLU28_03255"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOG34115.1"
                     /translation="MQEKKYSPVDFYEFNKSMFDLIFDIKRFLLSEDIPPNLSLMEYL
                     VVFTVYFYGPMKMRDISQKVTLTQSAITVIVDKLEKEGYVRRERDSDDRRVINIISDE
                     KGIEFCDQGLQNLKKMYDKGIEVLTDKEQESFLRMLTKISDSLKQQK"
     gene            complement(13586..>13925)
                     /locus_tag="PLU28_03260"
     CDS             complement(13586..>13925)
                     /locus_tag="PLU28_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34116.1"
                     /translation="SFEEINSGITTIDKETNAVLQEFKGILGQIQLMASMTENVSASA
                     QEQSAAAEEMASGMDNAAKVIVSISEQIKAMAQAVEQQAIGAQQTSTESQDLSQLSEN
                     LSAIIKTYKY"
BASE COUNT         4557 a         2413 c         2900 g         4055 t
ORIGIN      
        1 gttgaaaagg cacgtggaag ttcaaaaaaa tataatcaac tataaagagg tgattttatg
       61 gatattttaa ttaaaaaggg ttttatatgt ccggttgtca gcaaagcttt tacaggtgat
      121 attctgataa gcggtggaaa gatcaaaaaa atttccgagt ctattactgc caaagatgta
      181 caggttattg atgcaaaagg aaagtttgta cttcccggat ttatcgatgc acactcacat
      241 ataggacttt tcgaagaggg agtaggacag tactatcagg acggaaacga agctaccgat
      301 cctattacgc cacaagttag ggcaattgat gcatttaatc ctcaggacag tgcgataaaa
      361 aaagctctga gcggaggagt aacgacaacc atgatacttc ccggaagtgc aaatgcagta
      421 gggggtcagg gagcgataat aaagtttaac tctcagattg ttgatcagat gataatcaaa
      481 gaacccgcag gactgaagat ggcactgggg gaaaatccca agcgcttcta cggagaaagt
      541 atgaaacgta ctcccgggac caggttggga acagcagcca tgataaggga gtacttcgta
      601 aaaactcaga gctatagtga aaaaaagcaa aagggtgatt atgaaaagga tctgaggtat
      661 gagataggtg agatggtgct taaaagaaag attcctgcca gaatacatgc ccacaggatg
      721 gacgatattc tgacagctgt ccgcatagca aatgagtttg aatttgattt tgtaattgaa
      781 catgccacgg aagcatataa gataattgag tatcttaaag ccagaaatgt aaagatggta
      841 ctcggtccgc tcttttcatt tgccactaag cttgaactga aggataagac ttttgaagca
      901 gtttccatag cggttaaaaa caatgttcct gtggcactca tgtgcgatca tccggtggtt
      961 ccgatggaat ataccataat gcagctcggt acggctctga aatacggagt atctgaagag
     1021 gagcttttga agcttgtcac cataaatccc gcacggattc ttggaataga cgacagcaca
     1081 ggttcactgc aaaaagacaa ggatgcggat atagcaatct ggtcaggaaa tccttttgaa
     1141 atgaagagct gtgtggagca ggtcttaata cagggagagt gtgtattctc cagataacgg
     1201 cggtggaaag ttgaagtatg ccaagataaa aacagcttgt ataaatggat ttgatgttcg
     1261 tgaaattatt gttgagctag atataaactc acgttcaacc atacagacct tcaaaattgt
     1321 aggcatggct gccacgacag ttcttgaaag cgaaaagcgt gcgatgagct gcattaggaa
     1381 ctgtggtttt accgttccga acggaagcat aacggctaat ctttctccaa gttctctcag
     1441 aaaagaggga acccattttg atctgccggt agctgttgct cttttacagg catcttcaca
     1501 gattaaatcg gcagatggtg atttctattt tttcggagag cttggactta acggagaaat
     1561 ccgtccggta aacggtatcg ccctttttct tatttctttg aagcagtcca atcctcaggc
     1621 caagtttatt attcccaaag aaaacgaaaa tgaatcttac ttcattgatc caaatgatgt
     1681 gtatgtaata tcctcactta acgatatatg cagaattttt tcagaggaaa gagaaagttt
     1741 tcttcctcat gttggttcat gtgcaggaaa aacctttgat atggattttt ctgaaataaa
     1801 aggtcaactt ttcataagaa gagcagttga gatagcatgc agcggttttc ataatatact
     1861 tctcaagggc agtcccggtt ctggaaagac tatgattgca aggagaattc cgactatact
     1921 gcctcctatg acgaccgagg agattattga gtcgaccaaa atatactctg tatgcggata
     1981 tataaataag ataataaatg aaagaccttt ccgttcacct catcatacag cttcctcggc
     2041 atcggtaatc gggggtggaa atatccccag acccggtgag ataagtcttg cacataacgg
     2101 tgtacttttt ctagatgagt ttcccgagtt tcgcaatgat gtcatccagt cgcttcgtca
     2161 gcccatggaa gacggtatgg ttactgtcac aagggcacga tcggttgctg aattcccagc
     2221 aaggttcatg cttgtagccg ctcaaaaccc atgtccgtgt ggttttttcg gagataaaga
     2281 aaaagaatgt acctgttcca ttaatcagat aagcagttat aacaagaaaa tatcaggacc
     2341 tataaccgac aggatagata taaagataga tgttcctagg gtagagattt cagatcttct
     2401 gggtaaggat gacggagagt cttctgaaaa aataaaagaa agagttatga aaacgtcttc
     2461 aatccagata aagcggcagg gtaagttcaa cggtcagctt accggtcggg aggtaaagga
     2521 gtatgctatc ctttctgaca aggcagaaga gtttctaaaa actgccgccg ttaagctcaa
     2581 gcttacagcc agatctgtaa ccaaaataat aagagttgcc agaacagttg ccgatactta
     2641 cgaatctgaa cagatacaaa ccgttcatat tggagaagct cttaattaca ggaatgttga
     2701 aattaattaa ttttttatct gcataagcca atggagtaac gtaaaacata cgcaatatta
     2761 accttaaaac aatctttgtg cgataaaata aatactaata ttcctataaa cggtttcttc
     2821 attcttataa tggaataata ggaggcgtag gtatgaaaag tatacgttct aggcttatgt
     2881 tttggctgat aactcttttt acagctatac tgcttatcgc cggagtaata ctttacgcac
     2941 agactcagtc aacagtagta cctacagtgg aaaagatggc cgagcagata gttattgcca
     3001 gagccgatga aataggggaa tggcttaacg gtattctgat agaggtatct gcccagtcgg
     3061 acagaaatgt tgtaaaaaca atgagttgga ctaacatgca ggagtctctt cttagataca
     3121 ttaacgataa aaataaaatt tttgaatctt tatgggtgtg tgatcttaag ggtgacttca
     3181 catcaacctc aggcagaaca ggaaatatct attctgcaga gtactttgaa gcaatagcga
     3241 aaaagaatct caattttttt ataaccaatg tgatgtactc gcaggagacc aaggagccta
     3301 tatttgttgt aactaagagc attcttaatg ataaaaagga gctaatagga gtaatgggag
     3361 ccaatgtgct tattaacacg gtatctgaaa ttgtaggcag tataaatata gacaacagcg
     3421 gatacggata tatagtagac ggaaccggta tggttcttgc ccatcccaaa aaagaagtgg
     3481 ttctaaatat gaatcttctt aaaggaaccc aacttggtta caaaggcctt gaagatatag
     3541 gccagcagat gattgaagga aaggccggga tgggaatagc ctctctcaca gacggaacca
     3601 aaaacgttac atttttttca ccggttccct atgctccaaa ctgggcacta gcagttgctg
     3661 taccgcaaac atatctgtac aggacgtcaa atgcacttgc aatgactctt atacttctca
     3721 tactggttct tatagtcata agcataggtc tgtcggtttt tataggagga acaatagcca
     3781 atcctattaa aaaacttatg aaaaagatag atacctttgg atcaggagat cttacagtcg
     3841 actttgagct taagcagaag gatgaggtag gaaagatagc ctcgtcgcta aagagtatgg
     3901 cagcctcact taaacaggca atgttcagta ttcaggaagc ttcgggacag atgcagtcct
     3961 cgtcatctga tctggctgct attgccgaag aaaccagcgc aacgagtgag gagctttcct
     4021 cgcaggtgga aagcatatct tcaaattatt ctcagacaaa ccaatcaatc acagaggtaa
     4081 aggaagctgt agtacaagtg gccaaaaatt cgcaggaggt tactcagtct tctttgaatc
     4141 tgcttcagag taccagaatc gtcaaaggct ccgtagaaac aggagagcag tctataggaa
     4201 gtatagcagc acttgtacag gagtcttcgc aaaaagcgga tgaaaccaaa aagcttcttg
     4261 acaggctcaa ggataataca gataatgtag gaaaaatagt ggatacaata aactccataa
     4321 ctgagcagac caatcttttg gcacttaatg cagctataga agcggcacgc gccggagatg
     4381 cgggaagggg ctttgcagtt gttgcagatg aaataagaaa gcttgccgag gagagtaaaa
     4441 aggctacagc caatattgcc aacattcttt cggaaaccag agagtacgct caggatagca
     4501 aagagtcctt tgatgagata gccggtaata tacacaatat ctccaacgaa acattgaagg
     4561 taaaaaaaga gtttaacgga atactcggac agataagcca gatatcttcg atgaccgagc
     4621 aagtgagcaa gaactcgcaa agtcagagct cggcatctca ggagatagcc tcttccgttg
     4681 aaaattctgc taagatggct gatgatatta acttccagct caacagtaca gtcacagcca
     4741 taagtcagca ggctttagca gcccagcaaa tcggtgccaa ctccgaagaa cttagtgcac
     4801 tggctgaaac cttatcccag caggtgaaaa aatttaaaac atgaaagacg gttctgaaca
     4861 gccaggtaat tactttataa aaattcttat aaatattttt ttagtatcaa tatttctaac
     4921 aattaattta ttcataattt ttatagaaag ctctcacata aaggaactta ctttttcctt
     4981 aggtgagagt gtttctcgtg atataagttt gagaatgaac aacagttttt catcacttat
     5041 tgctttgggg tatacctttg aaacagaagg agagcttacc cagaataggt ttgaagctct
     5101 tagccgctac tttataagag agtttcccaa tgtatctttt ttacagcata aaaatgctca
     5161 gacagttaca gatatggtct atcccctatg gggaaatgaa gctactattg gacagtcgct
     5221 catggataat gaacaggcgc tcaaatcact tgaaaaagca aaacagacca gaactctgac
     5281 agttaacgat ccatatatgc ttaagcaggg ttatcttggg atgatagcca gatatcctgt
     5341 ttttgtagat gatgagtttg caggattata tgtgtgtggt attaatcttg atgaattttt
     5401 aagcttcctt tcaaaaaaag agatttttgg cagttatgat tattcttttt ttgattcgac
     5461 aggaaaagta ttttttgaaa gcagtacaga aaacaagaag aacttttcac aggagttcaa
     5521 aatacctata gccgactctt actggatact tgagatatac ccaaaaaatg attatttgat
     5581 gaacttcatt ctttattcga ttattttatg gggattgtat cttttggtct ccttctttgt
     5641 tggttcgttg gaacataggc ttaacaggaa agatcagctt ataaaagagc tgaaagacct
     5701 taaaataaag ctgaccgaag aggcatatga aaaggaaact attgcagccg acctgcgtaa
     5761 gggtcaggag ctttacagaa acatgtttta cagtaatact tccatcatgt ttcttattga
     5821 tccccagact ctttatattt acgatgccaa tcctgcggca gtaaagtttt atgggtatcc
     5881 tttggaggag ttcaaaaagc ttaaaattag ccagataaac gttcttggtg atgaagttgt
     5941 tcataaatcg gttaattcgg tgattgactc taatttttcg tttttctcct tcaagcataa
     6001 gctttctgac ggcaccataa gggatgtaga agttcatgca agcaaggttc agataaacga
     6061 taacgcttat atctattcaa taattcatga cgttacttcg agaataaaaa tgcaggaaaa
     6121 gttgaatctt attgccaggc tgcagtcgat agttgtggat atagcaacta aagtcataaa
     6181 tctttcttca ttctatatag aagacactgt aaacagtgcc ttggaagaac taggaaagct
     6241 tttggaagct gaaagaatag ttgttttttt taatgaagat aaaagcagtt ataaaaattg
     6301 ggaatggaag tctcctgaaa gttcttcaaa agatgtgtgc agtacataca gtgattatga
     6361 agatatttta aaacagctgg ataaaaacga taaggacttt ctttttatca ggtcatcctc
     6421 ctgctcgtca gtgttcatac caatgaatac cgacaaaaaa atttcgggat ttatagctat
     6481 gtccggaaat tctaaggagt ataactttga tcctcaggtt ttgaaagctg taaagcttat
     6541 tgctcagacg atagcctctg gaataaagcg taaagagtac gaaaatataa ttctttcgaa
     6601 aaaagaagaa gctgaaaagg caagctatgc caaaacagca tttctatcga gagtcagtca
     6661 tgaattgagg actcctatga acggaataat tatgggctgc cagatcgctc aggattgtga
     6721 cagtctgcag gaggtaaaag aatatacaga gcttataaaa aaatcagcag acagtcttat
     6781 gtcacttatt gaagacctat tggatctatc caagatagaa gcaggagatg tcgatcttca
     6841 gatacttgac ttcaatgtgg aaaatgtgtt aaggtatcta gaggaggagt atcgttccag
     6901 agcaaaggat aaaggacttg acttcttctt ttcagttgat gaaaagatgc ctttaaaaat
     6961 atacagtgat ccgggaaaga ttatgcggat actaaagaaa cttctggata attcgataaa
     7021 atttactgag aaaggtttta taagtctttc agttgagaat gttcatcgtg aaagagagta
     7081 tgtcagattt aaagtaagcg attcaggtgt gggaatagaa gaggaaaagt atgggtttat
     7141 ttttcagcag cttgtgcagc ttgaggaaca cagtgcacgg aagtattttg gaaccggaag
     7201 cggtcttttt atagctaaga aactggttaa gcttctgggc ggagaaatat tctttgaaag
     7261 tgatttcgga aagggaagca ctttctatgt tgagataccc tacaaaccta tatgatggga
     7321 gcgattccat gatgagagta gaaggcacaa acggagcagc tgattggata agcagatata
     7381 aaagcctatc aaaaggaaaa gacggtacag aggctaaatc ttcagaaaag gttaaaaaca
     7441 ggataactca aaattcctca gagcaggaaa atgattctga catcgacata agaactcaga
     7501 agaaactggg tattaaagaa tgtgaaacat gtgctaaccg taagtatcag gatggttcaa
     7561 ctgatggggg agtatctttc aaagcacctg cacatatatc gcctcaggca gctccagcag
     7621 ctgtacgctc tcatgaacag gaacatgtgg caaatgaaca ggccaaggct caatcttccg
     7681 gaaaggaagt aatttcacag tatgtacgta tatttaccgc agtttgtcct gaatgcggga
     7741 gggtctatgt aagcggcgga gaaactgtta ccacaactat ggataagaaa aattcaggat
     7801 ttgaatatga cagctcttta aagtactctc aaaaagcaca aggaaatttt tttgaccgaa
     7861 gtgcataact tcctaaaatc aaagcttttt ttatacaaat aagaatatat aaatgacgat
     7921 gtggttattt gccatcaccg tcattttttt atttattcaa aataatctat aattttaatg
     7981 attgaaaaat ttaactattt aaaaaactga taatgtggag gtttaagtat gcgttcgaaa
     8041 tttctacgaa tagctgtagt cgggatattt tctgcagtaa ccatattttt tgcccttaga
     8101 ctgcttaaca taaagatcag tgacgatata atgacctata tgtctgaaga aaataaagaa
     8161 atttcttttt acagggaagt ttctgaaaaa tttgatattg ataacattat gatgataagt
     8221 gttgagatga acagtgtcta tgatgagctt aatgaggtgg aaagcataag gaaagagtta
     8281 tactccttaa aagatgtcaa gaaggttctt agcataacca actctttaat cgtcagctct
     8341 gacgagtatg agataaagtc tggatcactc agtacaattc ttgatcttag ttcttacaat
     8401 gttgcagaag tagaaaaaat tcttgaaacg gacagttcac ttaaagatac atttgtttcc
     8461 aaagacggaa aaaatgttct ttatatttta aatcttgatg attctaaaaa agttgatatc
     8521 ggcttgcttt tggaggaaat agaaaaaact ctttcgacaa ggacgtacta tctcacagga
     8581 ctcccacttg ccaactatga gatagcccaa acggcttttt cagatgttgt cagaatcctt
     8641 ccgtttgcaa ttttgggaat aattgcaata ttatttacgg tgttcaggtc ctttgccgga
     8701 gttatgcttc ctattgtggc ggttattatt tccaatgcag ttgccatggg attggttaca
     8761 atcttcagcg agtcattttc cacagtaacc ataatgatgc ccataattct tgtgggtata
     8821 ggtgtagaca atactgtgta tctcatttca aggtataatg aagagtatcg acatgatgag
     8881 gacaaaaatg ctgcggttac aagggcaagg aaaaaaattc ttacccctgt acttatgtgt
     8941 acactaacaa ctgtggccgg ctttatgtct cttatcactg caaggctgat tcctgtaact
     9001 cagcttggtt tttatacatc ggccgggatt attgtttctt tcctggtagc agttgttttt
     9061 atgcctgcac ttattttgat tgtaaagcct agggcaaaac agagcagcga taaggaaaac
     9121 agaaagagct tgtgggcggc aatcaccctt aaggtcataa aacacaaagt gcttgtttca
     9181 actgttatgg tcattatatt tttatgtttt attcttatca tcccgggcat aaaaacagaa
     9241 tctaaccttg aaacttacct tggaagcagt gctctgacga taaaaggctc taattttata
     9301 aatgaaaagt tcggtggtga caagttcatt aatgtataca tgaaatcagg aattgaagaa
     9361 ggatacaaag atttttacta caatagggtt caaagagata tccaaaagta tgttcttaca
     9421 attgacagcc atttcataag tcgtggtctt tctacattca cttcttctgt tgtaaaaagt
     9481 ttctccggaa ctgagcatat tcctggcaac agcagtacga tgaaccaggt tttcctgatt
     9541 atagaaaaca ctccagagct aagcaagttt cttctcaaac cccaaagcga atcggtgata
     9601 gatgtcagga taagcagtaa caacgataca caggttcagg ctattctgga aaaactcaat
     9661 ggatttatag aaaagtatgt gtttaaaagc tacgataagg ctttacttga tatcaacaat
     9721 cctaaagctc tcaaggagta tgaaaagtca ctgcagagtt tttttgtttc caggaatatt
     9781 tcacatgcag ctgaaatgtt ttcaatgctt gcagagaata agaaaaaaag cttgtctcag
     9841 gttgtaaccg aataccctga aaatgaactt tatgggaagt atcaatcatt ctgtgaaaac
     9901 ttttctctcc aagccgatga gaaaagtata tttttaaaaa atattaaaaa tgaagagtat
     9961 accgaaactt ttacaaactt tctatctttt aatgaacaaa ctctcagaag cagagttttt
    10021 tatgaagagc tcaagaaatt ctttcctgcg gtgcaagatg gagatcttgc agagttttcg
    10081 gagtatgtga atgaccgtga gtatgtcacg gatacaggat caatcccatt atatctgaaa
    10141 gtgacaggaa atcctataat aagaaccttt ataaacagtg agcttcttta cggacagatg
    10201 accagcctgt ttttttccta tatttttata ctattgatgt tcattatatc gtccagatca
    10261 gttcgtacag gtcttatagc ttccctccca ctattgttta caacagtggc taattttggg
    10321 tttatacggc tattcaactt cagtcttaat gccggaacta taaccattgc ctctatagtt
    10381 ataggtcttg ccattgatta ttctgttcat tacgtatcaa gattcagaga ggagttttta
    10441 aaaaccatgg atatctgtaa atctctagaa ataacttcaa aaactgttct tagggcaata
    10501 agcaccggtg ctttcactac tcttctgggt ttttttccgt tgggatttgg aaatctcgga
    10561 ataatgaaac agttcggaat aatatcaggt ataagcatcc ttatttcgct tttgtccacc
    10621 gtagttattc tacctattgt attttcctca ataaaaaaag aaacactttt aaaaatactt
    10681 cagaaaagga aggcggataa aaatgaaaaa aagtcttttt ttctttctgt ttttaaccgt
    10741 taccgctagt atattcggct taactgctca ggatgtgctt gataaggtta aggactcaca
    10801 gaataacttt gaaacgcaga aggcagtata ctcaattatt ctgtctgaca gcaacaagac
    10861 tcagaaaaca agaaagatag atatctatat ccttatatct tctgataagg gagagaagct
    10921 tcctatatct cttctgaggg ttaccgagcc taaagatctc caaaatacaa ctgtgctgaa
    10981 tctttcaaaa accgaacagt atatctatct ccctggattt aagcaggtaa aaaaggtttc
    11041 cggttcttca aaaaatgaaa gctttcttga taccgatata aattattcgg atatgacact
    11101 tcttttttca tcaaccattg aacaggagca cacaagctct atatcctcgg aagatgaaaa
    11161 aagctatacc atagatataa aacttgccgc acaggacagt gattattcca agatgacggt
    11221 tggggttgat aagaaagatt ttgtgataaa gtatgcagag ttctttgaca agaaggaaca
    11281 aatagttaaa aagatggaga ttatggatta tgaaagctcc ggggagtata taacttacaa
    11341 aaaagtactt ataacttccc tggagaacaa acacagcaca cttttggagc ttgtcagttc
    11401 ggagtatgga attcctataa caaagagctt ttttaataag ctttcaatga ccaatccggt
    11461 attggtatac aggtaggtga ggctgatatg aaaaaagtga tggtttttat atgtactatc
    11521 tgggctgcag gcgttattat gggtttttcc ggaaagtcca tttacacgct tggatacaac
    11581 tatcaggttg attttgttga tatataccag ctgttgaata cagacataga gtttgagtcg
    11641 cagaatgaaa gcagctactt taacttatgc ataaacatta cagcacttaa cggtctctct
    11701 cccgaaagca ccaatgtata tattgactcg taccttcttg gatataagga tataaaagcg
    11761 tttttcccgg gattggatga gatactggta aacagtatct tctatgggga aataaaagaa
    11821 ctttactatg atcagtactt ctcgggaaac actctgagag tggggagatt ttatcccaat
    11881 aaagccagtg caaatctgta tagtccgtca aatgtaatgc aagcaagcaa ctttctggga
    11941 tacctcaatg aaaatactct tacgggcatt gatgggctgg tgcttcagct ttttttcgaa
    12001 cagatgagtg ctgagtgcta ctttacacct gttaccaagt atgattttcc taatcttaca
    12061 gcttatgaaa aaacacagtc gatatacgac ctgcttttag aaaaatcagg aagtgaaagt
    12121 gattatctgc ttttggctta taagtacaag gatagaaatg tactgcgtct tgatgatatg
    12181 aatacaggat tgaagattga tttttcgatt tttgattttt ctgtcagacc gggatacttc
    12241 agaaataact atcattttga tattccggag cttgtcgaag tggattttat taaaaacgac
    12301 ataatgctta acctccatat aaacgatata accatagacc tttcgaagta tgaatttccg
    12361 ccggaaatta aagataaccc tatttttgcg gagcttctga aacaaaaaat tatctttgat
    12421 acggactact ctacatcaat gacaataaac acaagcgcag caacgtcaac aattataaat
    12481 ccttgggcag acgtaataac agcggatata cagtattcat caaaacttat tgaagggctt
    12541 acatggtatt ttgaagccgg tctgtatctc ccgcagcaga ttacaaccaa aggtgagtac
    12601 tcaaagataa agcgtcagga taccatagat ataacctttg aaggtaccac aaatgttttt
    12661 tacagctata ccaagcaaca gaacaaagag gcatatgttc cgaatgtctt ttccgagagc
    12721 cttcttggaa acagatatgt aaagtacatt gcaggatttg agtatctgac aggtgatttt
    12781 tcattcggat tagaattttt taacggaatg aaccaggaac acttttcaat gactcccgga
    12841 atagatggat tttttaacta cagtaccaaa atatttgata ttaacctttt tggtcttgtc
    12901 acttttcctg aaatacatgg taaaatagat attggttata ccacggtagc aaaagccgcc
    12961 tataccggga tggatcagac gcgaattggc ttaaagagca catactcgta cagcgaaaat
    13021 gaaaagaata ttatatatac ttcaaagatg cttaattcag tggagttttt cattgaaaag
    13081 tacttttaag gagctggttt ggttgcaaga aaaaaagtac agtccggttg atttctatga
    13141 gttcaataaa agcatgttcg atctgatatt tgatataaaa agatttcttc tcagtgagga
    13201 tattccgcct aacctttcac ttatggagta tctggttgtt ttcacggtgt acttttacgg
    13261 tcctatgaaa atgcgagata tatcccaaaa ggttactctt acccaaagtg ccataacggt
    13321 tattgtcgac aagcttgaaa aagaaggata tgttaggagg gaacgtgatt ctgatgacag
    13381 aagggttatc aatataattt ctgacgagaa aggaattgag ttctgtgatc aaggtctgca
    13441 aaatcttaag aagatgtacg ataaaggtat agaggtactt acggataagg aacaggaaag
    13501 ttttctcaga atgcttacaa aaatttcgga ttcacttaaa caacaaaaat aaaataagag
    13561 ggcttaagcc ctcttatttt atttatcaat acttataagt tttaattatg gctgagagat
    13621 tttctgaaag ctgactcaga tcctgacttt cagtactggt ctgttgagct cctatggcct
    13681 gctgttccac agcctgtgcc atggctttta tctgttcgct tatagatacg attactttgg
    13741 ctgcgttgtc cattccgctg gccatttctt cagcggccgc actctgttcc tgggcacttg
    13801 cagatacgtt ttcagtcatg cttgccatga gctgtatctg tcccaaaata cctttaaact
    13861 cctgtaggac tgcgttggtt tctttatcta tagtggttat gcctgagttt atctcttcaa
    13921 aggac
//
LOCUS       DAOSHR010000036        13673 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_3727_length_13673_cov_5.701057, whole genome shotgun sequence.
ACCESSION   DAOSHR010000036 DAOSHR010000000
VERSION     DAOSHR010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 13673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13673
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3727_length_13673_cov_5.701057"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /gene="prfB"
                     /locus_tag="PLU28_03265"
     CDS             <1..219
                     /gene="prfB"
                     /locus_tag="PLU28_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296881.1"
                     /note="recognizes the termination signals UGA and UAA
                     during protein translation a specificity which is
                     dependent on amino acid residues residing in loops of the
                     L-shaped tRNA-like molecule of RF2; in some organisms
                     control of PrfB protein levels is maintained through a +1
                     ribosomal frameshifting mechanism; this protein is similar
                     to release factor 1; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOG34117.1"
                     /translation="MRKKMEEKMKLQGDVKEISWGNQIRSYVLYPYQMVKDHRTEFES
                     SNSQAVLDGDINGFLEAELLFFADVSKE"
     gene            288..1175
                     /gene="rsmH"
                     /locus_tag="PLU28_03270"
     CDS             288..1175
                     /gene="rsmH"
                     /locus_tag="PLU28_03270"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOG34118.1"
                     /translation="MREYYDYHKSIMVPEVLEYLNIKDDGIYVDCTAGEGGHCKAIYE
                     KAGGKARVVGVDVDYEVLEIAEQRLKDIGCKIDFFKASYKDIDIVLRGMGIKKVDGFL
                     MDLGVSTFQLKGENRGFTFMNDEPLDMRMDPDSVFSAIQVVNKYEEEELARIIFEYGQ
                     EYQFSRKIARSIVSSRPVNTTGELVKAIQHGLPSYEIHKRKRHFATKTFQAIRIEVNK
                     EFENIKAALGKFENLLNHGGRVVVISFHSLEDKIIKDYFRNNPSYSLVTKKPVLPQES
                     EISSNPRARSAKLRVAEFK"
     gene            1199..1477
                     /locus_tag="PLU28_03275"
     CDS             1199..1477
                     /locus_tag="PLU28_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34119.1"
                     /translation="MSERASTLRNVRERAGSSVNFLDLAIYVSLFMAAMVISIWLVMF
                     LNLGKDIESYKKIIDSKTASFENITEVVKSTDLKIEQYIQLLSVLNGK"
     gene            1485..3188
                     /locus_tag="PLU28_03280"
     CDS             1485..3188
                     /locus_tag="PLU28_03280"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOG34120.1"
                     /translation="MKIRYSIARLFIAGLYLFVVAAGLFFNTELLSINAANKNSSDDS
                     QKKIATLVDSKGHFIVYEKPKYEAWLDIEFMKRQKKYAALITKLQTRFLYSEIENKKY
                     ICWGTYETLELAKKDLGELYKFANIHRSAERVYNNLFSLNLMIGKYDQTTYGIEKYLY
                     NKGMLSGKDRVTVTLDMVLQKIAYEEIKRVKDALSAEGAIAIVMETASGKIRASAAVY
                     PWNLGFMGYIEPGSTFKPLIYALAIDEKISNPYESILCERTYQPVQDVSYKMKEAENE
                     DFGLIDIKEALSKSSNIAVAKTMKKIMNVFDENWLYQKLLNMGLGEKTGVEFEGEISG
                     ALAHPDKWYKIMPFQIAIGQGIGVTPIQLVSAFNILVNSGNYVKPTFVEETEIIPKRV
                     FTQETSKLLVDWMKYTTLTGTARLAYKEGIAIGGKTGTAQKALAEVGYASEKYYALFA
                     GFYPVTDPKYTIVVIIDDPKGEKYYGGEVAAPIATNIFYRYFKETDAGHESAGSLFRE
                     VIPDLTGQDVQEAVYILNALGIPSDKIIISGSGKKVISQEPSQALFISDIQFIQLFLG
                     E"
     gene            3193..4170
                     /locus_tag="PLU28_03285"
     CDS             3193..4170
                     /locus_tag="PLU28_03285"
                     /inference="COORDINATES: protein motif:HMM:NF017911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34121.1"
                     /translation="MSISIVSGSSYIKKENSIKKLWESFQGQKTLVSRENYTKSSLEE
                     VFSTKGMFSQEKLVIIKELDSFKAKEVKDVIRILSLADGNDKILITVTAETEDIKKIK
                     ADEKIDAALPKPWEDNEWKDFLDSIAAQFGKKIEKHAAEYLLQNLGREDTYMYEEISK
                     ISIYAQTDTITLEDAVQIGYTFSDPQLEDMCYLMACKKTDKVMEMLDKIIDDSEFNAP
                     ATVGFMYRYFLDLYKVILNAPGLKKYTWANIQKIAKDCGIKNSSRVRSFLGVTFQNDI
                     IKKINLENMYDPSKLRRIIIMFEQLDRQAKSTDNFKVAFSVFFREAFVE"
     gene            4163..5125
                     /gene="earP"
                     /locus_tag="PLU28_03290"
     CDS             4163..5125
                     /gene="earP"
                     /locus_tag="PLU28_03290"
                     /inference="COORDINATES: protein motif:HMM:NF021598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HOG34122.1"
                     /translation="MNKNIDIFCSVIDNFGDAGYTLRVAASFKNQYRNSLINIYTDCT
                     ELFFKLMPKEGSPQINVFSMDSSSDYHASEVVLGMFQFFPDERYIDFINRESKLFIGV
                     DYFTPELWAKDIKALPLMHQGLRIKTLFFVPNVHAESSGILRFSWKSQGLPTDVLYPR
                     YISNAYLYDYGPVEKLLPQESVFNWKINTQNRFFTQEEFDEILYSSKYNWIRGEDSFS
                     LALWSGIPFFWQAYKQKETRHFKKVWAFNEFIKPFFEDAQMYKRYVNVVNTLNGIYNN
                     DVTDDFLYIDKKYGQLKDVFEKMKEYFLKQKTLQQNLMENIEYL"
     gene            5183..5740
                     /gene="efp"
                     /locus_tag="PLU28_03295"
     CDS             5183..5740
                     /gene="efp"
                     /locus_tag="PLU28_03295"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOG34123.1"
                     /translation="MIFAGELKVGYVIKYNGGLYTIQKAKYNWSGRNEATMTVKMKNI
                     ENASTIDIVMKGSEKLEDVQLDHRDMKFIYASGDSYAFMDEESYEQIEVFADDLGDVL
                     NFLKEDGKVIVSFYESRPVAYALPKTVTLEVTYTEPGEKGDSSGNPLKPAKVETGYEL
                     GVPLFVNIGDKIVIDTKTGEYLSRA"
     gene            complement(5785..7026)
                     /locus_tag="PLU28_03300"
     CDS             complement(5785..7026)
                     /locus_tag="PLU28_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOG34124.1"
                     /translation="MSSFYEEEKLNAAISDMAELSSLITGTENEEEFFKKLLDVALKS
                     IPQAECATIWKIEGTLYRPVAGFPYERDILSKMVISYEDSYAYNHNIDECIVQTNNIL
                     DYYSGKNEKFYDLLSSIKTGRNRMCTIIAPLKVNAKIAGHIFIDNFSLDSFEDVAKRA
                     LKIFSNFASTFLTLKTLKDSETEANELNRVYLSFITHELKTPLTSIIGFSETILDESN
                     MKLNAMRGYIRKIYASSRHLNSLIDDLSTFNKLNRETSLNIRRINFKQTLFEAVSIVE
                     PNISPDVELEISYSADIPAFIETDPTKFTQVLVNIIGNAIKYTDEGSIFVHSSFDSST
                     KEFVISVRDTGIGISQEKLEDIFRPFYRVSKNRPGSGLGLAIVKKTIQVLKGRISVSS
                     EEGKGSEFLIRLPLVIKTDEQ"
     gene            complement(7125..8846)
                     /locus_tag="PLU28_03305"
     CDS             complement(7125..8846)
                     /locus_tag="PLU28_03305"
                     /inference="COORDINATES: protein motif:HMM:TIGR03904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HOG34125.1"
                     /translation="MLASTAEEMEKLKIEQFDVILVTGDAYVDHPSFGTAIIGNVLSQ
                     IAGLNVGVISQPDWKNAGDISRLGRPKYFFGITAGNVDSMVANYTASRKKRKTDEYTP
                     DAQFGKRPDRACVVYSNLIKGVFHGIPIILGGIEASLRRLAHYDWWQNRVKKSVLLDS
                     KADLIVYGMGEKTILQIAEIFKNGGNIRDCTSLRGTVYWSSALPQNTSDFTVLPSFET
                     VSENKNEYMKMFKEFSANNDPYDSKGLIQKHDNRYVVQNRPQLPLSQHELDRVYGLDY
                     TRKVHPFCLSQGYVKGIDTVRFSITSHRGCYGGCSFCAITLHQGRIIQSRSISSILRE
                     AEQISKDEKFNGYISDIGGPTANMYGHDCLKKVELGSCKEKQCIGDEVCHTLNPSHKA
                     YIDLLSQVEKLPFIKKVFISSGIRLDLIYADKKYGMAFLRRLVELNTSGQIKIAPEHA
                     SPNVLRLMNKHSFEIVRKFISDYESLNKELNKNQFFSAYLIAAHPGAELQDEVFLRKE
                     IEKYFGFSPQQVQIFTPTPGTMSTAVYYTELDPFSSAHVFVEKDDKKRKMHKDILLGE
                     SNTKKHY"
     gene            complement(8900..8976)
                     /locus_tag="PLU28_03310"
     tRNA            complement(8900..8976)
                     /locus_tag="PLU28_03310"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8940..8942),aa:Asp,seq:gtc)
     gene            complement(9080..9967)
                     /locus_tag="PLU28_03315"
     CDS             complement(9080..9967)
                     /locus_tag="PLU28_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34126.1"
                     /translation="MKRLFVFIFMLSLSISLLAYSFFNPIGPALLPAAGLYSENTTDL
                     TKDYWRTLDEAQILALKQQADFLVLPVAYGAELISKGFEYKLAGVSIWKSFYLVTSQE
                     VNSIEDLRGKRIVTIHSPGQTGDLILKMITKQKNINFETVYVSSGADIIQLLATGKET
                     MAVLPEPFVSLAEVKTKNVFTAKMDLQQVYAQLTGYPAMIPITGLFVSNKVPREYAQK
                     IIDRYEQSSNKYFKDEFDKSIKFMFDLMDGKMPDPVLRKASQRSFIEFGKDKEIVLGF
                     LKILKENGAISDYKQDMFF"
     gene            10181..11350
                     /locus_tag="PLU28_03320"
     CDS             10181..11350
                     /locus_tag="PLU28_03320"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOG34127.1"
                     /translation="MKLYVSDMHIGSGNQKDDFVYDAKFIELLDKYEDQITEFFIVGD
                     FLELMHVKDKKILCETVQECMDLFDHEILNKIYNAHTALFDRLKKLSSKVRIRYIAGN
                     HDYHVLFSEKIRQKVFSMTGCEEVLPFYYDEDWEIFVIHGNQFDAVNRLSYDGKNNII
                     PTFGEYMAKFMSSNFDIKMSKILPDELISDYDNIRPLLDVFVWLDYINERYCLNYNLK
                     EEWISEFINLINAKQTRNWLKINFPKYYRFTDFFVNKFGGMKLGESLVRTVMYFREFK
                     KSDMYLKAAKRLLENNFLIPRKNLVGYSDEDISLSNDKVKGLIMGHCHRPSYNQVNCG
                     KIKKFYANTGAWKHVVMRNKGVNDNEFIKRSTVSYLVIDEYNKNLHAKLSVEEFY"
     gene            11483..>13673
                     /gene="ppdK"
                     /locus_tag="PLU28_03325"
     CDS             11483..>13673
                     /gene="ppdK"
                     /locus_tag="PLU28_03325"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HOG34128.1"
                     /translation="MAKKYVYFWGKGKSEGNAKMKELLGGKGANLCEMDRLGLPVPAG
                     FVISTEICDHYWKNNKNFPLTLKEEVTNCMKRLEAESGKKFGSKENPLLVSVRSGASI
                     SMPGMMDTILNLGLNDETVQALAKNTGNERFAFDAYRRFMQMLGDVAIGIPHEKFEHA
                     LKSVKAEKGIKLDTELNAQDLKKVVELYKEIYKQEGKEFPQDPEEQLWIAIEAVFGSW
                     MNERAIKYRAINGIKEGTLLGTAVSVVMMVFGNMGEDSGTGVAFTRDPNTGANYIYGE
                     YLPNAQGEDVVAGIRTPFKLDKLHEISPKVHDSLITLMKNLERHFKDMQDIEFTVEKE
                     NLYLLQARNGKRTAAAAVKIAVDMVKEGLISKQTAVTRVKPEDVDKLLHPFFDTVSLK
                     EAKYIGKGLPASPGAACGKVVFSADVAEEMISKGEGPVILVRPETSPEDVGGMNAAEG
                     IITSIGGMTSHAAVVARGMGKTAVVGATSIVINDKEKYLEANDIRVNNLEWISIDGSE
                     GKVYAGKVKTVKPQGLEGEIAELLAFADEIAEVGVRANADIPRDAKVARSFGAKGIGL
                     CRTEHMFFEGDRIMKFRRMIVSETVEQREAALEQLLPVQKDDFKGLFESMDGYAVTIR
                     FLDPPLHEFLPNDEEQMKELAPQLGITVDHLKEVVESLHEFNPMMGHRGCRLSITYPE
                     IAVMQTKAIIMAAIEVAKAGKKVRPEIMVPIVGFVEEFEFVKKIVVET"
BASE COUNT         4488 a         2296 c         2860 g         4029 t
ORIGIN      
        1 atgcgaaaga agatggaaga gaagatgaag ctccagggtg atgtaaaaga gatttcatgg
       61 ggaaaccaga taaggtcata cgttctttac ccttatcaga tggtaaagga tcacaggacg
      121 gagtttgaat cttctaactc acaagctgtg cttgacgggg atataaacgg atttttagaa
      181 gccgaactgc ttttttttgc tgatgtttca aaagaataag gggtttgacg taccggcaga
      241 ggcagatagt ttacaattga caaaactgtt aaggcggtgt ataatttttg cgagagtatt
      301 atgattatca taaaagtata atggttccag aagttttaga gtatctgaac atcaaggatg
      361 acggaatata tgttgactgc acggccggag aaggcgggca ctgcaaggct atatacgaga
      421 aagccggcgg gaaagccaga gtggtaggcg ttgacgttga ctatgaagtt ttggaaatag
      481 ctgagcagag gctcaaagat attggctgta agatagattt ctttaaggca tcatataaag
      541 atatagatat agtgttaaga ggaatgggga taaaaaaagt tgatggtttt ttgatggatc
      601 tgggagtttc cacttttcag ctcaagggag agaacagagg atttacgttt atgaatgatg
      661 agcccttgga tatgaggatg gatccagatt cagttttcag cgctatacag gttgtgaaca
      721 aatatgagga agaagaactg gcaaggataa tctttgagta cggccaagag tatcagtttt
      781 cacgtaaaat agcaaggagt attgtttcct caagacctgt caatacaacc ggagagcttg
      841 taaaagccat tcagcatggt ttaccgtcgt atgagattca taaaaggaag aggcattttg
      901 ctaccaaaac ttttcaagct ataagaatag aagtcaacaa agagtttgaa aatattaagg
      961 ctgcacttgg aaaatttgag aatcttttga atcatggcgg aagggtagtc gtgatatcct
     1021 ttcattctct tgaggataag ataataaaag attatttcag aaacaaccct tcatattctc
     1081 ttgtaactaa aaaacccgta ttaccgcagg aaagcgaaat aagcagcaat ccaagggcaa
     1141 ggagtgccaa attaagagtt gcagagttca aatagctctg ttttaagggg gaaggtacat
     1201 gtcggaaagg gcatctactt taagaaatgt aagggagaga gcaggtagtt cggtcaattt
     1261 tcttgatctg gccatatatg tatctctttt tatggctgca atggttataa gcatatggct
     1321 tgtgatgttt cttaacctgg gcaaggatat agaaagttat aaaaagataa ttgattcgaa
     1381 aaccgcttct tttgaaaata ttactgaagt tgtaaagtct actgatctga agattgagca
     1441 gtatattcaa ttgctgtcgg tgctgaacgg aaagtaggtg ttttttgaag ataaggtatt
     1501 ccatagcccg tctgtttata gcagggctgt atttgtttgt ggtagccgca ggtctttttt
     1561 tcaatactga gcttctcagc ataaacgctg ccaataaaaa cagttctgat gactctcaga
     1621 aaaaaatagc gacccttgtt gacagcaagg gtcattttat tgtttatgaa aagcctaagt
     1681 atgaagcatg gcttgatata gagtttatga aaagacaaaa aaaatatgcc gctcttataa
     1741 ccaagcttca gacaagattt ttatactcag aaatagaaaa taaaaagtat atctgctggg
     1801 gtacatatga aaccttggaa ctggcaaaaa aagatcttgg tgagctttac aagtttgcta
     1861 atatccatag gtctgcggaa agggtctaca ataatctttt ttcacttaat cttatgatag
     1921 gcaagtatga ccagacaaca tacggtattg aaaagtacct ttataataaa ggaatgctca
     1981 gcggtaaaga tagagtcaca gtcactctgg atatggtgtt acagaagatt gcatatgaag
     2041 agataaaaag agttaaggat gccctttcgg cggaaggggc aatcgctatt gtgatggaaa
     2101 cagccagcgg aaagataaga gcttcagctg ctgtgtaccc ttggaatctc gggtttatgg
     2161 gatatatcga acctggatca acttttaaac cattgatata cgctttggcc atagatgaaa
     2221 agatatccaa cccttatgag agcatacttt gtgagcgaac ataccagcct gttcaggatg
     2281 tcagttacaa gatgaaagaa gctgaaaatg aggattttgg attaatagat ataaaagaag
     2341 ccctttcaaa atcatcaaat attgctgttg cgaaaaccat gaaaaaaata atgaatgttt
     2401 ttgatgagaa ctggctttat caaaaacttt taaatatggg tctgggtgaa aaaacaggag
     2461 tagagtttga aggtgagata tccggagcac ttgcccatcc tgacaaatgg tacaagataa
     2521 tgccgtttca gatagctatc ggacagggta taggagtaac ccccatacaa cttgtttcgg
     2581 cttttaatat ccttgtgaac tcaggaaatt atgttaagcc gacatttgta gaagaaacag
     2641 agattatacc caaaagagtt tttacgcagg aaacatccaa gctccttgta gactggatga
     2701 aatataccac attaacgggt actgcaagac ttgcatataa agaaggaata gcaatcggcg
     2761 gtaaaacagg aacagcgcag aaagcattgg cggaggtagg gtatgcatcc gaaaagtact
     2821 atgctctgtt tgcgggattt tatccggtaa ccgatcctaa atatacaatt gtggtaataa
     2881 tagatgatcc gaaaggtgaa aagtactatg ggggagaggt cgcagctcct attgcaacca
     2941 atatatttta tagatacttt aaagaaacag atgcaggaca tgagtcagct ggaagccttt
     3001 tcagagaagt aattcctgat ctcaccggcc aggatgtaca ggaggctgtt tacatactta
     3061 acgcattggg tataccctct gataagataa tcataagcgg aagtggtaaa aaagttattt
     3121 cccaggaacc atctcaggcc ctttttataa gtgatattca gttcatacag ctttttttgg
     3181 gagagtaaag atatgagcat atctattgta tcaggaagtt catacataaa aaaagaaaat
     3241 tcaatcaaaa aactttggga gtcctttcag ggacaaaaaa ctcttgtaag cagggaaaat
     3301 tataccaaaa gctctcttga agaagttttt tcaaccaaag ggatgttcag tcaggaaaag
     3361 cttgtgatta taaaagagct tgactctttt aaagccaaag aagtaaaaga tgttatcaga
     3421 atactttcgc ttgcagacgg taacgacaag atacttataa cggttacagc ggaaactgaa
     3481 gatataaaaa agattaaagc cgatgaaaaa atagatgctg ctcttccaaa gccatgggaa
     3541 gataatgaat ggaaagattt tttagatagt attgccgcac agttcggcaa aaaaatagaa
     3601 aaacatgcgg cagagtatct tctgcaaaat cttggaaggg aagatactta tatgtacgag
     3661 gaaatctcaa aaatcagcat atatgcacag actgatacaa taactttgga agacgctgtt
     3721 cagataggct atacattttc agatcctcag cttgaagaca tgtgttatct tatggcctgc
     3781 aaaaaaacag ataaagttat ggaaatgctt gataagataa ttgatgacag tgaatttaac
     3841 gcacctgcaa cggtagggtt tatgtacagg tactttctag atctgtacaa ggttattttg
     3901 aatgcaccgg gattgaaaaa gtatacttgg gcaaacattc agaagatcgc caaagactgt
     3961 ggcataaaaa acagttcaag agtaaggtcg tttttagggg ttacatttca gaacgatata
     4021 ataaaaaaaa taaatctcga aaatatgtat gatccttcta aattacgaag aatcataata
     4081 atgtttgaac agctggacag acaggcaaaa agtaccgata attttaaagt ggctttcagt
     4141 gtgtttttca gggaggcgtt tgttgaataa aaatatagat attttttgca gtgtgatcga
     4201 caattttgga gatgccgggt ataccctgag agttgcggca agtttcaaaa atcaatacag
     4261 aaactcttta attaacatct acacagactg tacggagctg ttcttcaaac taatgcctaa
     4321 ggaaggatct cctcaaatca atgtcttttc tatggatagc agtagtgatt accatgcttc
     4381 ggaagttgtt ctgggaatgt ttcagttttt cccggatgaa agatatatcg attttataaa
     4441 cagagaatcc aagcttttta taggagttga ttacttcacc ccggagcttt gggcaaaaga
     4501 tataaaagct ttgcctttga tgcatcaggg tcttagaatt aaaacattat tttttgttcc
     4561 gaatgttcat gcggaaagtt cgggtatact gagattttcg tggaaatctc aaggtttgcc
     4621 tacggatgtt ttgtatccgc ggtatatttc caatgcttat ttgtatgatt acgggccagt
     4681 agaaaagctc ttaccgcagg aatcggtttt taactggaag attaataccc aaaacagatt
     4741 tttcacgcag gaagagtttg atgaaattct ctactccagc aaatataact ggataagagg
     4801 agaagattct ttttcgcttg cgctctggtc aggaattcct tttttctggc aggcatacaa
     4861 acagaaagag acaaggcatt tcaaaaaagt atgggccttc aacgaattta taaagccttt
     4921 ttttgaagat gcacagatgt ataagcggta tgttaacgta gtaaacacac ttaacggaat
     4981 atataacaac gacgtaacag acgatttttt gtatatagac aaaaaatacg ggcagttgaa
     5041 ggatgttttt gaaaaaatga aggagtactt cttaaagcag aaaactcttc aacaaaattt
     5101 aatggaaaat attgaatatt tatagtataa tgttataaaa tctttttgaa attgcatatt
     5161 tgagaattgg aggtattaag aaatgatttt tgctggtgaa ttaaaggtag gatacgtaat
     5221 aaagtataac ggtgggctct ataccattca gaaagccaag tacaactggt cgggaaggaa
     5281 tgaagcaact atgacggtta agatgaagaa cattgaaaac gcatctacaa tagatatagt
     5341 catgaaaggc tctgaaaagc ttgaagatgt tcagctagac cacagggaca tgaagttcat
     5401 atatgcttcc ggagactcat atgcgtttat ggacgaagaa tcttacgagc agattgaagt
     5461 atttgcagac gatttgggtg atgttctcaa cttccttaag gaagacggaa aggttattgt
     5521 ttccttctat gaaagcagac ctgttgctta tgcacttcct aagactgtta cccttgaagt
     5581 gacatacact gaacctggtg aaaaaggtga ttcaagcggc aatcctctta aaccagccaa
     5641 agtggaaaca gggtatgagc ttggtgttcc tttattcgtc aatataggcg ataaaatagt
     5701 tattgatacc aaaaccggag agtatctctc aagagcatag ttgaaaatca ttgaacaata
     5761 aaaagatgcc ttaaaggcat ctttttattg ttcgtcggtt tttattacaa gcggaaggcg
     5821 tataagaaat tcgctgcctt ttccttcttc tgagcttacg cttatccttc cttttagaac
     5881 ctgaattgtc ttttttacta tagctaaacc caatccgctt cctggcctgt ttttagaaac
     5941 tctgtagaaa ggcctgaata tatcctcaag tttttcctgt gatattccta ttcccgtatc
     6001 ccgtacagat attacaaact cctttgtgct tgaatcaaag ctgctgtgaa caaaaatact
     6061 tccctcatcg gtatacttta tggcattgcc tattatattg acaagaacct gggtgaactt
     6121 ggtgggatct gtttctataa atgccggaat atccgcacta taagatattt ccaactcaac
     6181 gtctggagaa atattcggtt caactattga tacagcttca aataaggtct gcttgaaatt
     6241 tatcctgcgt atattcaggg aggtttccct gttaagctta ttaaaggtag aaagatcatc
     6301 aatcaaagag ttaagatgtc ttgaagaagc atatatctta cgtatatacc cgcgcattgc
     6361 gttcaatttc atattggatt catccaggat ggtttctgaa aaccctatga tgcttgtaag
     6421 aggtgtttta agctcatgag taataaaact gagataaacc ctgttaagtt catttgcttc
     6481 agtttcagaa tccttaagtg tttttaaggt aagaaaagtt gatgcaaagt ttgaaaaaat
     6541 cttcaatgca cgtttggcga catcttcaaa ggaatcgagg ctgaagttgt caataaaaat
     6601 gtgcccggca atttttgcgt tgactttaag cggtgcaatt atagtacaca tgcggtttct
     6661 tcctgttttt attgaagata aaagatcata gaacttttca ttttttccgc tgtaataatc
     6721 aagtatatta tttgtctgca ctatgcattc atcaatattg tgattgtaag catatgaatc
     6781 ttcatatgaa ataaccattt ttgaaagaat atcccgttca tagggaaaac cagctacagg
     6841 acggtataaa gttccctcta tcttccatat tgtcgcacac tctgcctgag gaatgctttt
     6901 taaagcaaca tcaagaagtt ttttaaaaaa ctcttcttca ttttcagtgc cggtgataag
     6961 agaagaaagt tcagccatat cagatatggc agcattaagt ttttcttctt catagaaaga
     7021 actcatttca cactcctcct aagatattgt gaatacaaaa atggaagtac ttcatagata
     7081 ttttatcata aaaaatacaa aaatgtaatt atttttagac ttttttaata atgttttttg
     7141 gtatttgatt cacccaataa aatatctttg tgcatcttcc tcttcttatc gtctttctca
     7201 acaaatacgt gagccgatga aaacggatca agttctgtgt aataaacagc agtagacata
     7261 gtacctggtg taggcgtgaa tatctgtacc tgctggggtg aaaaaccgaa atacttttct
     7321 atttctttac gcaaaaatac ctcatcctgt agctctgctc ccggatgcgc tgctatcaga
     7381 taagctgaaa aaaactggtt tttgttaagt tccttgttaa gactttcgta gtctgaaatg
     7441 aactttctga cgatttcaaa agaatgtttg ttcattaatc tcaaaacatt tggacttgca
     7501 tgttctggcg ctatttttat ctgtccggag gtattaagct ccacaagacg tcttaaaaaa
     7561 gccattccgt attttttatc agcatagata agatccagcc gtattcctga agagatgaac
     7621 accttcttta taaaaggaag tttttcaacc tgtgaaagaa gatctatata ggctttatga
     7681 gaaggattca aagtatgaca gacttcatcc cctatacact gcttttcttt gcagctaccc
     7741 agctccactt tttttaaaca gtcgtgtccg tacatattgg cggtgggacc accgatatca
     7801 gatatataac cgttaaactt ttcatctttg gaaatttgtt cagcttccct gaggatagat
     7861 gaaatgcttc tgctttgaat tattcttccc tgatgaaggg taatggcaca aaagctacag
     7921 cctccgtaac agcctctgtg cgaagtgatt gaaaatctta cggtatcaat acccttgaca
     7981 tatccctggc taagacagaa gggatgtact tttcttgtat aatccaggcc gtatacacgg
     8041 tcaagttcat gctgagacaa aggcagctgt ggcctgtttt gaaccacata tctgttatcg
     8101 tgtttctgga taagtccttt tgaatcataa ggatcattat tggcggaaaa ctctttaaac
     8161 attttcatat actcattttt attttcagat acagtttcaa agcttggaag aactgtaaag
     8221 tctgaagtat tctgaggcaa tgctgaggac cagtaaactg ttcccctaag agaagtacag
     8281 tcgcggatgt ttcctccgtt tttaaaaatc tcggcaatct ggagaatagt cttttctccc
     8341 atgccgtata ctataagatc ggcttttgag tcaaggagaa cagacttttt tacacggttc
     8401 tgccaccagt cgtaatgagc aagtcttctc agacttgcct cgatgcctcc gagtattatc
     8461 ggaataccgt gaaagactcc ttttatgagg tttgaataga cgacacaagc acggtctgga
     8521 cgtttgccga actgtgcgtc tggggtgtac tcgtcggtct ttcttttttt tctggaagca
     8581 gtatagtttg caaccattga atcgacattg cctgcggtta ttccgaaaaa atattttgga
     8641 cggccaagtc ttgagatatc accagcgttt ttccaatcag gctgggaaat aactcctaca
     8701 ttcaatccgg ctatctgtga aaggacattt ccgattattg cggttccaaa ggaaggatga
     8761 tctacgtatg catcgcccgt caccaaaata acgtcaaact gttcaatttt aagtttttcc
     8821 atctcttctg ctgtagatgc aagcatactt atcctcctaa caaccaaaag acctcctttt
     8881 caaaggaggc cttcattatt ggcggggacg acgagactcg aactcgcggc caccggtgtg
     8941 acagaccggc atgctaacca actgcaccac atccccaaca ttccttaact tcgcacgttt
     9001 attattctat cagaaaaaat gtaacgtatc tagccctttt gtgtgtctta tgagtaaaca
     9061 agtgttaagg aaataaaaac taaaaaaaca tatcttgctt ataatcagag attgcgccgt
     9121 tttctttaag aatctttaaa aaacctagta caatctcctt atctttccca aactctataa
     9181 aagaacgttg agaagccttt ctgagaacag gatcaggcat tttgccgtcc atgaggtcaa
     9241 acataaattt tatagactta tcaaactcat ctttaaaata cttattagat gactgctcat
     9301 acctatcaat aattttttgc gcatactctc tgggaacctt gttggaaaca aataatcctg
     9361 ttataggtat catcgccgga tatcctgtca actgtgcgta tacttgctga agatccattt
     9421 ttgcagtaaa aacatttttg gtttttactt ctgctaaaga tacaaaaggt tccggaagta
     9481 ctgccattgt ttcctttcct gttgccagaa gctgtattat atctgcaccg ctgcttacat
     9541 atacggtttc aaagttaatg tttttctgtt tggtaatcat tttcaatata agatcaccag
     9601 tctgtcccgg tgaatgaata gtaacgattc tctttcccct taaatcctct atagaattaa
     9661 cttcctgtga ggtaacaaga taaaaagact tccatattga tactcctgcc agcttgtatt
     9721 caaagccttt acttataagc tctgcaccat aggccaccgg tagcacaaga aaatctgctt
     9781 gctgttttaa agcaagtatc tgtgcttcat ccagcgttct ccagtaatcc ttggtgagat
     9841 cggtagtgtt ttcggagtag aggcctgctg caggaagcaa tgctggtcct atgggattaa
     9901 agaaactgta agctaataga gatatactta gtgataacat aaaaataaaa acgaataatc
     9961 ttttcataat aaacctcctg tagatgtttt cctgtaaaac cttttgtggt ataatcaaat
    10021 acttcttaat tataacatga attgtcggat ttaaaactcc ttcttacaaa gttaatgttt
    10081 ccgaaatgtt tatctaaaca acaaaggata taaatttgtt tgcacggtca gcattcattt
    10141 caattataga acataaaatt taccgtttga ggatgataat atgaaacttt atgtaagcga
    10201 tatgcatata ggatctggaa accagaagga tgattttgta tatgatgcaa aatttattga
    10261 actcttagac aagtatgaag accagataac agaatttttc attgtagggg attttcttga
    10321 acttatgcac gttaaggaca agaagattct ctgtgaaact gttcaggagt gtatggattt
    10381 gtttgaccat gaaattctga ataaaatata caatgcccat acggcccttt ttgataggct
    10441 gaaaaagctt tcttcaaagg taagaataag atatatagcc ggtaatcatg attatcatgt
    10501 tcttttttct gaaaagataa gacaaaaggt attctctatg acaggatgcg aagaagtact
    10561 gccgttttac tatgatgagg actgggagat atttgtgata cacggtaatc agtttgatgc
    10621 cgtaaacagg ttgtcgtatg atgggaaaaa taatataatc ccaacctttg gagagtatat
    10681 ggctaagttc atgtcatcta attttgatat caagatgagc aagatactgc ctgatgaact
    10741 catctctgac tatgacaaca taagaccttt actcgatgtt tttgtatggc tggactatat
    10801 aaatgaaaga tactgtctta actacaatct taaggaagaa tggataagtg agtttataaa
    10861 tcttataaat gcaaagcaga caaggaactg gctaaaaata aactttccta aatactacag
    10921 gtttactgat ttttttgtaa ataaatttgg cggaatgaag ttgggagaat ctcttgtaag
    10981 aacagttatg tacttcaggg agttcaaaaa aagtgatatg taccttaaag ccgcaaaaag
    11041 acttttagag aataattttc tgataccacg taaaaatctc gtgggataca gtgatgaaga
    11101 tataagctta agcaatgata aggtaaaagg tctgataatg ggacactgcc acaggccgtc
    11161 ctataatcag gtcaactgtg gcaagataaa gaaattttat gctaatacgg gtgcatggaa
    11221 acatgttgta atgagaaaca agggagttaa cgacaacgaa tttataaaga gaagtactgt
    11281 atcttatctt gtaatagacg agtacaataa aaatcttcat gctaaattaa gtgtggaaga
    11341 gttttattaa aaaataggaa tataacagtt aacatttttc atgtataata ttttcacttt
    11401 gaaaaaaaca attttaccga tcaattctgg tataataaat gagaaataaa gactcaggaa
    11461 tctattttta ggagggaaag atatggctaa gaaatacgta tatttttggg gcaaaggcaa
    11521 atccgaagga aatgccaaga tgaaggaact gcttggcgga aagggagcca acctttgtga
    11581 gatggacagg ctaggactgc cggttcccgc cggtttcgtt atatcaactg aaatatgtga
    11641 tcattactgg aaaaacaata aaaactttcc tcttacttta aaagaggaag ttaccaactg
    11701 catgaaaaga cttgaagcag agtccgggaa aaagttcgga agcaaagaaa atccgcttct
    11761 tgtttctgta agatctggag cttcaatatc aatgccagga atgatggata ccatactaaa
    11821 tcttggtctc aatgatgaaa ccgttcaagc acttgccaag aataccggaa atgaaaggtt
    11881 tgcctttgat gcctacaggc gttttatgca gatgcttggc gatgttgcaa ttggaattcc
    11941 tcatgaaaaa tttgaacatg ctttaaagtc tgttaaagct gaaaaaggta ttaaacttga
    12001 tactgagctt aatgcacagg atctcaaaaa agttgtcgag ctttataagg aaatatacaa
    12061 gcaggaagga aaagagttcc cacaggatcc tgaggaacag ctttggatag ctattgaagc
    12121 tgttttcggt agctggatga acgaaagggc aataaagtac agagcaatta acggtataaa
    12181 agaaggaacc cttcttggaa cagcggttag cgtagttatg atggtttttg gaaatatggg
    12241 cgaagacagc ggaaccggag ttgcatttac aagagatcct aatacaggag caaattatat
    12301 ctacggtgaa tacctaccta acgcacaggg agaagatgtg gtcgcaggta taagaacacc
    12361 ttttaaatta gataaactcc atgagatatc tcctaaagtt catgacagtc ttataactct
    12421 gatgaagaat ctcgagagac actttaaaga tatgcaggat atagagttta cagttgaaaa
    12481 agaaaatctg tatcttcttc aggcaagaaa cggaaaaagg acagctgctg ctgccgttaa
    12541 aatagcagtt gatatggtta aggaaggtct tatatccaaa cagactgctg ttaccagagt
    12601 aaaacctgaa gatgtagaca agctgttgca tcctttcttt gacactgtgt cgcttaaaga
    12661 agcaaagtat atcggcaaag gtcttcctgc ctctcctggt gcagcatgcg gaaaagttgt
    12721 attcagtgct gatgtggcag aagaaatgat ttcaaaagga gaaggtccgg taatacttgt
    12781 acgacctgaa acaagccctg aagatgtagg cgggatgaat gccgcagaag gtataattac
    12841 ttctataggc ggaatgacct cacatgccgc agttgttgca agaggaatgg ggaaaactgc
    12901 tgtggtcggt gcaacatcaa tagtaataaa cgataaggaa aagtatcttg aagccaacga
    12961 tataagagtt aataacctcg aatggatttc catagacgga agtgaaggaa aagtatatgc
    13021 aggaaaagtc aagactgtaa aaccacaggg gcttgagggc gaaatagcag aacttcttgc
    13081 tttcgctgat gagatagccg aagttggagt aagagccaat gctgatatcc caagagatgc
    13141 caaggtagca agatcctttg gtgcaaaggg aataggtctt tgcagaacag agcatatgtt
    13201 ctttgagggc gataggatta tgaagttcag aagaatgatt gtttctgaga ctgttgaaca
    13261 gagagaggct gctcttgaac agctgcttcc agttcagaaa gatgatttca aaggtctctt
    13321 tgaatcaatg gatggatatg ctgtaaccat aagattcctt gatccgccgt tacatgaatt
    13381 ccttccaaac gatgaagaac agatgaaaga actggctccc cagcttggaa taacagttga
    13441 tcaccttaaa gaagttgtcg aatcacttca tgagtttaac cctatgatgg gtcacagggg
    13501 atgcagactc tcaataactt atcctgaaat agctgtaatg cagaccaaag caatcataat
    13561 ggctgctata gaagtagcta aggcaggtaa aaaagtaaga cctgaaataa tggttccgat
    13621 agttggtttt gttgaagagt ttgaatttgt taagaaaatt gttgttgaaa ctg
//
LOCUS       DAOSHR010000037        13545 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_3788_length_13545_cov_5.552483, whole genome shotgun sequence.
ACCESSION   DAOSHR010000037 DAOSHR010000000
VERSION     DAOSHR010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 13545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13545
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3788_length_13545_cov_5.552483"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="PLU28_03330"
     CDS             <1..612
                     /locus_tag="PLU28_03330"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF015882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0004473 - malate dehydrogenase
                     (decarboxylating) (NADP+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HOG34129.1"
                     /translation="KNNFMVDLNGVLYKGKANINRIQQYLSESTNHQMIKGDLYTVVK
                     GADVLIGVSKPGAFDEKVIRSMNRDPIVFALSNPDPEISYHQAISFGARIAATGRSDT
                     PNQVNNVLVFPGIFRGAIDVRARKITDEMKINAAYAISGLVTEAELDFDYIIPSAYNP
                     NVAPNVAAAVSRTAVDQGLARKTIDIEKRRNELIQKFNISTKK"
     gene            739..1767
                     /locus_tag="PLU28_03335"
     CDS             739..1767
                     /locus_tag="PLU28_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34130.1"
                     /translation="MQSSYVLLNSDIFSQSASVGIILRMNPVRQDIFDSFACRLIVHI
                     HSSLKGIAYILSQRRSENYLYICISTFEKNIEHIFDALKRFEFHGTAYINKNSIYSLE
                     DRMLRNLFFMRNFFKEENTSEVLKGTFVFLNFSDTLAINSYMQELLEKKNIIKESADP
                     YEKKRIKILELPIQEVYLTLGYTQECQSIEKLVFAEIMSILFREKSPVIVNSQKASFM
                     NVYFDKRLFCGFVINTNQQSVQTDFAQLKSSFIRKVTSFTETDFIWGKNFLNLYLMHI
                     EENYFEKLRVKGIYEEYSIKDISYTLNNSNFCDFRDLLLQYTENKFYIGLYGNIEKEK
                     TDDIFFMT"
     gene            2037..2894
                     /locus_tag="PLU28_03340"
     CDS             2037..2894
                     /locus_tag="PLU28_03340"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOG34131.1"
                     /translation="MIANLLINGAVNASIYALIALGFSLTFGVAGIVNLYHGAYYMVG
                     AYLAYFFISIGIPAFFSVILSLLSVMLIGVFLMKYPILYLQRKGESIYILILTLGSAY
                     FTQYLFKLVFGTSHYSIKPFYDQSMLLLGEIIPGSRIFAFFVSVVSIVIVMFFINKTK
                     FGKLILATSQDKYAAQLLGVNTSLIFIVTSALSAGLSGLAGIVISPFLSINLGMWLPT
                     LIKAFSIVILGGLGSIWGSILAAIFMAYLEQFVALVWTSSMTEVVFLVIVFLFLVFKP
                     TGFMGKKVA"
     gene            2906..3862
                     /locus_tag="PLU28_03345"
     CDS             2906..3862
                     /locus_tag="PLU28_03345"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOG34132.1"
                     /translation="MKKFKDLRVILILIGIVFMFIFPVVFNDQYLIWVFSIALIYSVY
                     ASSWDLLSGYTGKENFGHAAFIAIGGYMVGFISKAFPIPVYLSLPISAIVSAIFGVVI
                     GIPTLRLKGPYFALATLAVASIFEKLTISMSQYTGGEEGMIGITYLTDDPNKSYYFLL
                     IFAVLAVYTMFKISRSNFGLILKALKSDEDACRAIGINTTFYKVSAFALSASFAGMAG
                     TLVAHNFGYIGPDIVYPLSLNSMIMAVVGGIGGIVPAALGGFFLTFASELLRDFGDYN
                     QIIYTLLLIFSALFLPNGVFNGLSNFLKPKGLKTSTEVRGGK"
     gene            3859..4608
                     /locus_tag="PLU28_03350"
     CDS             3859..4608
                     /locus_tag="PLU28_03350"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG34133.1"
                     /translation="MSILEIKNLNKNFGGLRASNDISFSLEKGERLGILGPNGAGKTT
                     IFNLISGFILPDSGEIIFNSKDIVGYQPEKIACMGLVRTFQIVKPFKDLEVYENIKVA
                     TLSPRMKQFIKNEKERKEWIDHVIEVCELSEVRKVKTQLLPQGYLKKLEVAKALAVKP
                     EVVLLDEPFAGLTHSEIEPIANVIIKINEKGTSIILIEHRLKEFMKIVERVVAVDYGQ
                     VIAQGTPEEIIKNEQVIESYLGKGGAALVNP"
     gene            4595..5302
                     /locus_tag="PLU28_03355"
     CDS             4595..5302
                     /locus_tag="PLU28_03355"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG34134.1"
                     /translation="MLILSLKNITAGYAKAVVLEDMNLDINEGECHAVLGPNGSGKST
                     LLKVITGSVFPAKGSIFFLGNEITGRKTFEIARMGVSLSPENRRLFPDLSVKENLEIA
                     MVYESTKSKKNSMEFVFEIFPRLKERITQKAGTMSGGEQQMLAIGRAIMLNPKVLLLD
                     EPSMGLAHVIKEQIFEGINKIRSTGKTILIVEQDATMVMPIADKISIFEHGKIKWEGN
                     PKDLTKDNSIRNAYLGI"
     gene            5320..6540
                     /locus_tag="PLU28_03360"
     CDS             5320..6540
                     /locus_tag="PLU28_03360"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOG34135.1"
                     /translation="MKKLFLLTLFSLLTVLTVLSASTWKIGVIATLQQENGVWVKNAV
                     DMAAKEINAAGGILGKKIELVYQDDENSAEKMKSGIMKLVTRDRIDFLIGGVGTGPVL
                     ASMDEMAMRKIIWLGTGAASPDIINFIRKDYNKYKYYFRVGTLDSIAQGRSIGEFIAN
                     SLVPKYGIKKAAIISVDLVYAKQIAETAAEIAKKAGVEIVYTDYFPAGTNDFSATFKK
                     ATDAGAQIIIDAIVTQDGIQFAKQWYDLKVNAALVGAIAAALKPEFYEQTSGKCLYET
                     SAYPNGGPAPLTTNTMRFFEEHKKIYSMAPGFISFPAYNALYVLKDALEMAKDPNNKD
                     DVIKSLEKVYFPMGGQKGVAPVVFDEFHDLRYGGEDGATGIIFQWQKNGAWKAVYPEK
                     YKTGDWEKPEWIKW"
     gene            complement(6571..8487)
                     /locus_tag="PLU28_03365"
     CDS             complement(6571..8487)
                     /locus_tag="PLU28_03365"
                     /inference="COORDINATES: protein motif:HMM:TIGR01561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HOG34136.1"
                     /translation="MSILKNYSEMEYLIANGLGGSSSACASGLNTRRYHSILSAALNP
                     PVNRRIFVSRASEIAVVEDSTYILSTLKTVNGFLESGYENILSFKYEGFPVWEFKTGS
                     NIIEKSIFMIKNRNMSVLFYKNIFGKELTLTITPYFNDRDVHSNTLPGSIRINILKFS
                     QNFVLFGTNFLNSISLKTDGEIQEYSHTLNNVFYDAENERGLFAYEEIISKVRITKKL
                     HPQESFYIIFGANDDVSDVDPFTALTSEKERIISISSSSSDNTIKRLLIAADTFIMKR
                     KSTGKHTIIAGFPWFTDWGRDTMIALEGLLLYTRNYSIAKEVIENFVNNLQNGLIPNV
                     FDDYSGQPKGYNTADATLWLFYALYKYYTFTHNDIFLKKMYPYLYEIIKKHISGTDYN
                     IHMDDDYLIYAGNKNTQLTWMDVKVEDWSVTPRYGKTVEVNALWFNALNIMTYFSKIL
                     NAEFEYEYLIEKVKSSFNEKFWNSKDNCLYDVINEEGKDFSFRPNQIFAVSLPFDILS
                     REKERLIIDKVLEELYIPYGLRTLSPKDPRYIGIYVGDRWTRDGAYHQGNAWPYLLGH
                     FLYAFLKINDFSKEAKIIVKKLLSPIEDMINTDWCGTVPEIIEGNWPHEKKGCFSQAW
                     SVGEILRIILEVNK"
     gene            8541..9176
                     /locus_tag="PLU28_03370"
     CDS             8541..9176
                     /locus_tag="PLU28_03370"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HOG34137.1"
                     /translation="MFDEYKDSVIEGCKRIVAENLVKGTWGNVSVSDGERIFITPSNF
                     PYEKLTRKDIAVTDMKGQIVLSEHKPSSELKMHIALYNARKDIKAVIHTHPVFSSAVS
                     LVCEKIPSLIEDSVMICGEEILVAQFASAGSEELAYNVVTAMGKNNATIMKNHGLVTI
                     GDSINEAITASLVCEKTAQIYIVALSCGNVSVISPEQATQLRQKYLKNYKQ"
     gene            complement(9178..10446)
                     /locus_tag="PLU28_03375"
     CDS             complement(9178..10446)
                     /locus_tag="PLU28_03375"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOG34138.1"
                     /translation="MPKYNYHDILVMIILTFEVKKMESITVKKGQYIYEAGSISENIY
                     RLVSGKIEISHNGNKNIIETGTFGEWIIAGAKCYDSAYTLTDCQIQILTPEDLNSLDS
                     KENIYFEMFESLTKRLTFLDECISSQIDQESAINTFKRFHPHHKLRTESKFLDFFSKM
                     KFLYNQENYQQALNEVYAFKSDYIDYPLRDELDIWKAIITFHLDPEKAKHLYNTFDHA
                     KYSKKLSFTYLKNLLENKNDTLLSLYEKYGIHVPPNTILIAEGENPNNEGYLLLDGGM
                     KVGRYSGKKEVLLSIIQKGDFFGEAGMFNSNCRMATVYTDVYCDVVKMSGEKLKSVIS
                     QFPALGMKMVKCQISRITFLDELFKTVCITNKRERITSIIDTYLEKFITVGITLNEFT
                     SLTNCTQREVLEIFSNRKIKIDSTGHIKTS"
     gene            10460..10993
                     /locus_tag="PLU28_03380"
     CDS             10460..10993
                     /locus_tag="PLU28_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOG34139.1"
                     /translation="MLNKEEATELLYQNISTETLIKHSRAVGAIMKHLAVFLGFDPVK
                     WEVTGLIHDLDYELTKHNVDQHALKTVEMLKGAIDEEMENAVLAHNDKKVLNAPIEIA
                     LYAADPLSGFITAAVLVIPSQDIRDLKVSSLKKKFKDKSFAKGASRENILKIEQLGIT
                     LESFFEIALKAFKEESL"
     gene            11048..11305
                     /locus_tag="PLU28_03385"
     CDS             11048..11305
                     /locus_tag="PLU28_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HOG34140.1"
                     /translation="MNLKDLEFYSLAEAKAKFSKVMDETKKKNIVITKNGLPQAIIVD
                     YDKFVSLMEFVDQIKDLYLVEAGDLSRVKEIEKFFLDFNEE"
     gene            11320..12423
                     /gene="ugpC"
                     /locus_tag="PLU28_03390"
     CDS             11320..12423
                     /gene="ugpC"
                     /locus_tag="PLU28_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HOG34141.1"
                     /translation="MADVILEHIDKVYPNGFKAVKDANIEIKDREFVVLLGPSGCGKT
                     TTLRMIAGLEDISAGTIKIGDKIVNDIEPKDRDIAMVFQNYALYPHMSIYDNMAFGLK
                     LRKMPKDEIEKRVKEAARILDIEHLLDRKPKQLSGGQRQRVAVGRAIVRNPKVFLFDE
                     PLSNLDAKLRVQMRSELKKLHSRLNATIVYVTHDQVEAMTMADKIVIMKDGIIHQIGD
                     PFEVYHKPSNMFVAGFIGTPAMNFLNAKLSTQNGSMWLTAENMNIKVPKKFEDILKPY
                     IDKEVVFGIRPEDVYDKLYAVAATDNNTVTSTVDVVEPLGSETLLHLTIGKQVITAKV
                     DPKSMAKSDQKLDIVFDMEKMHIFDKDSQQAIF"
     gene            12640..13476
                     /gene="cas6"
                     /locus_tag="PLU28_03395"
     CDS             12640..13476
                     /gene="cas6"
                     /locus_tag="PLU28_03395"
                     /inference="COORDINATES: protein motif:HMM:TIGR01877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HOG34142.1"
                     /translation="MILKMVFEPATDELVDLPVHYNRPLQGMFYNLLSQYFGSSINSD
                     EREKKIFTFSRIYPYESFRVDNKRLKFKGSFMVYFASPIEELMDCVSKAFSEKELFRI
                     ENNLFRLKSHVILKTEVNSPIMVKTVSPITVYSTITLPNKNKYTHYFSPYSDNFRNLI
                     SENIKRKASVLGIDFSEDNTFDISPYGVTEKNEKLLFYKGIIIKGWTGYFVLSGNKNL
                     IRLAFDSGLGAKNSQGFGMIIPADHNQAYEAIDKSDDSILTDDYKPHDVYEKEVFSNT
                     VI"
BASE COUNT         4481 a         2085 c         2532 g         4447 t
ORIGIN      
        1 aagaataatt ttatggtaga cttaaacggt gttctgtata aaggtaaggc aaatattaac
       61 agaattcaac agtatctttc ggagagtact aaccatcaaa tgattaaagg agatctctat
      121 acagtggtca aaggggcaga tgtgcttata ggtgtttcca agccaggagc ttttgacgaa
      181 aaagtaatta gaagtatgaa ccgtgatccg attgtttttg ctctttctaa tccagatcca
      241 gaaatatctt atcatcaggc tatttcattt ggtgccagaa ttgctgcgac aggacgttct
      301 gatacaccca atcaggtcaa taacgtactt gtttttcctg gtattttcag aggagctata
      361 gatgtcagag caaggaagat aacagatgaa atgaagataa atgctgctta tgccatatct
      421 ggtctcgtaa ctgaagcaga acttgatttt gattatatta ttcccagtgc atataacccc
      481 aatgttgcac cgaatgttgc tgctgcagtt tcaagaactg cggttgatca aggtcttgcc
      541 agaaagacaa ttgatataga aaaacgaaga aacgagctta tacaaaaatt caacattagt
      601 acaaaaaaat aatcccgttt ttacgggatt ttttgttaat agtattctat gttaaattca
      661 agtttttttt gtaaattaca tgatataatt atgtaaagag aaaaaattgt tatttaaaat
      721 atggaggatg ataatcttat gcagagcagt tatgtgttgc ttaattcaga tattttttcg
      781 caatctgcct ctgttggtat aattttaaga atgaatcctg ttagacaaga tatatttgat
      841 tcttttgcat gccgacttat agttcatatt cattcttctc ttaagggtat tgcttatatt
      901 ctctcccaac gtcggagtga aaattatctt tatatttgta tttcaacatt tgaaaaaaat
      961 atagaacata tatttgatgc tttgaaaaga tttgagttcc atggaacagc atatattaat
     1021 aaaaattcta tttattcact tgaagatcgt atgcttagaa atctattctt catgaggaat
     1081 tttttcaaag aggaaaacac ttcagaggtt ttaaagggta cttttgtttt ccttaatttt
     1141 tctgatactc ttgctataaa ctcctatatg caggaacttc tagaaaaaaa gaatataatt
     1201 aaagagtcag ctgatccata tgaaaaaaaa aggattaaaa tacttgaact tcccattcaa
     1261 gaagtttatc ttaccttagg ctatacacag gagtgtcaaa gtatagagaa acttgttttc
     1321 gcagaaataa tgagtattct tttcagagaa aaaagtccag ttattgttaa ctctcagaaa
     1381 gccagcttta tgaacgtcta ttttgacaaa agacttttct gcggttttgt cataaataca
     1441 aatcagcaaa gtgttcaaac cgattttgct caattgaaaa gttcatttat acgtaaagtc
     1501 acatctttca cagaaaccga tttcatatgg ggtaaaaact ttttgaatct ctacctcatg
     1561 catatagaag aaaattactt tgaaaaactt agagtaaaag gtatatatga agagtattca
     1621 attaaagata tttcttatac gctcaacaat tctaattttt gtgatttcag ggatttgctt
     1681 cttcagtata cagaaaataa attttatata ggtctatatg ggaacatcga aaaagaaaaa
     1741 actgacgata ttttttttat gacttgacgg gtaatttgaa ttattttcag attgcatctt
     1801 ataattttga aaagaagaaa atatttaatg taatgaaaac aaagtaattt ttatgtaata
     1861 tatgtttagt tatggttatt cggacatatt acctataatt tctaaataaa taccaaatat
     1921 cgagtttggt atttattttt tgttaagtat aatatataca tgagatatga ttcacatatt
     1981 acatgagaaa tgaatcatat cgtattaaaa atattatttt gagaggggtg acactggtga
     2041 ttgctaacct gcttatcaat ggtgcagtaa atgcttccat atatgcactg atagcattag
     2101 gtttttcgct tacttttgga gtagcgggaa tagtaaatct ttatcatgga gcatattaca
     2161 tggtaggagc ttaccttgcc tattttttta taagtattgg cattcctgcc tttttttcgg
     2221 taatactgtc tcttttatcg gtaatgctga tcggtgtttt tttgatgaaa tacccgatac
     2281 tgtatctcca acgaaagggt gagagtatat atattctcat acttacatta ggttcggcat
     2341 actttacaca gtatcttttc aaacttgtct tcggaacaag ccattatagt ataaaaccgt
     2401 tttatgacca aagtatgctc cttctgggtg aaataattcc aggttcaagg atctttgctt
     2461 tttttgtatc ggttgtttct atagttattg tgatgttttt cattaacaaa acaaaatttg
     2521 gaaagttgat acttgcaact tcgcaggata agtatgcggc gcagctgctt ggagtaaata
     2581 cttcactgat atttatagtc acctcggctc tttcagctgg attatctgga cttgctggga
     2641 tcgtaatatc tccctttctt tcaataaatc ttggaatgtg gctgccaaca ttaataaaag
     2701 ctttttcaat agttattttg ggaggacttg ggagtatatg gggaagtata cttgcagcaa
     2761 ttttcatggc gtatcttgaa cagtttgttg cattagtatg gacatcgagt atgaccgaag
     2821 tggtttttct tgttatagta ttcttatttc tggtgtttaa acctacaggc ttcatgggaa
     2881 aaaaagtagc gtaaggcggt gaattgtgaa aaaatttaag gatttaagag ttattttgat
     2941 tcttatagga atagtattta tgttcatttt tccagttgta tttaatgacc agtatcttat
     3001 atgggtattt tctatagctc tcatatactc agtttatgct tcaagttggg atctactttc
     3061 aggttatacc ggtaaagaaa attttggaca tgctgctttt attgcaattg gtgggtatat
     3121 ggtaggtttt ataagcaaag cttttcctat tcctgtatat ctatctcttc caatatcagc
     3181 aattgtatca gctatttttg gcgtagttat aggtatccca actctcagac ttaaaggtcc
     3241 ttactttgct cttgcaactc ttgctgttgc atcgatattt gaaaaactta caatatctat
     3301 gtctcagtat acaggtggag aagaagggat gatagggata acctacctta ctgatgatcc
     3361 aaacaaaagc tattattttc tgctcatatt tgcagtactt gcagtttata cgatgtttaa
     3421 aataagcaga tcgaattttg ggcttatatt aaaagcttta aaatcagatg aggatgcatg
     3481 cagggcaata ggaatcaata ccacttttta taaggtttcg gcttttgcac tttcggcatc
     3541 ttttgcagga atggccggta cacttgtagc acataatttt ggctatattg gtcctgatat
     3601 agtatatccg ctttctctta actctatgat tatggcagtt gtaggcggaa taggcggaat
     3661 tgttccagca gcgcttggag gattttttct tacatttgct tctgaacttc tgagagattt
     3721 tggtgattat aaccagataa tatatacact tttacttatt ttttcggcat tgtttctacc
     3781 aaacggtgtt tttaacggac tttcaaattt tttaaagcct aaaggactaa aaacaagtac
     3841 ggaagtaaga ggtggaaaat gagcatactg gagataaaaa atcttaataa aaattttggt
     3901 ggattaagag caagcaacga tataagcttt tctctggaaa aaggtgaaag acttggtata
     3961 ctcggtccta atggtgcagg taaaactact atatttaatc ttatatcagg attcatactt
     4021 cctgatagcg gagaaataat ctttaatagt aaagatattg tgggatatca accagaaaaa
     4081 attgcctgta tggggcttgt aagaactttt cagatagtta aaccatttaa agatctggaa
     4141 gtatatgaaa atataaaagt tgctactctt tcacccagaa tgaagcagtt tataaaaaat
     4201 gaaaaagaga gaaaagaatg gatagatcac gtaattgaag tatgtgagct ttctgaagtg
     4261 agaaaagtaa aaactcaact gcttcctcag gggtatttaa aaaagctaga agtagcaaag
     4321 gctttggctg ttaaacccga agttgtttta cttgatgaac cgtttgcggg gcttacacac
     4381 tctgaaatag agcctattgc taatgtaata atcaaaatta atgaaaaagg tacttctatt
     4441 attcttattg agcataggct gaaagagttt atgaaaatag tggaaagagt agttgcagtt
     4501 gattatggtc aggtaattgc ccaaggtact cccgaagaaa taattaaaaa tgagcaggtt
     4561 attgaatctt atctgggaaa aggaggggca gctcttgtta atccttagtt taaagaatat
     4621 aaccgcaggt tatgctaaag ccgtagtact tgaagatatg aatcttgata taaacgaagg
     4681 tgagtgccac gctgtgcttg gaccaaatgg ttctggaaaa tcgactttgc ttaaggtaat
     4741 caccggttca gtttttccag ccaaaggaag tatttttttc ttaggtaatg agatcacagg
     4801 cagaaagaca tttgaaattg cacgaatggg agtttcactt tcgcccgaga acaggagact
     4861 ttttccagat ctgagtgtga aggaaaatct cgaaattgct atggtttatg aaagcacaaa
     4921 aagtaaaaag aacagtatgg aatttgtttt cgaaattttt ccaagactta aagaacgaat
     4981 aactcagaaa gccggtacta tgagtggtgg tgaacagcag atgcttgcga taggaagagc
     5041 tataatgctc aatccaaagg tgcttttgct ggatgaacct tcgatgggtc ttgcacacgt
     5101 tattaaggaa cagatttttg aaggaataaa caaaataaga agtacaggaa aaaccatact
     5161 tattgttgag caggatgcaa cgatggtaat gccaattgct gataagatat cgatatttga
     5221 gcacggtaag attaagtggg aaggtaatcc caaagatctt acaaaagata actctattcg
     5281 taatgcctat ttaggcatat aatataagga ggatggaaga tgaaaaagct ttttttatta
     5341 actctttttt cacttttaac tgtattaaca gtactttctg cttcaacatg gaagattggg
     5401 gtaatagcta ctttacagca ggaaaatggt gtatgggtaa aaaatgctgt tgatatggct
     5461 gcaaaagaaa taaatgctgc aggtggaata ttaggtaaga agatcgaact tgtatatcag
     5521 gatgatgaaa atagtgctga aaaaatgaaa agcggaataa tgaagttggt tacaagagat
     5581 agaatagatt ttcttattgg aggagtaggt acaggacctg ttttagcttc aatggatgaa
     5641 atggctatga gaaaaataat atggctagga acaggtgcgg caagccctga tataataaat
     5701 tttatcagaa aagactataa taaatacaaa tactatttta gagttggaac tttggattct
     5761 atagcccagg gaagatcaat cggtgagttt attgctaact cactcgttcc gaaatacggt
     5821 atcaaaaaag cggctattat atctgttgat ttagtatatg ctaagcaaat agcggaaaca
     5881 gcagctgaaa tagctaaaaa agcaggcgtt gagatcgtct acactgatta ttttcctgca
     5941 ggaacaaatg atttttctgc aacttttaag aaggcaactg atgcaggagc tcagatcata
     6001 atcgatgcaa tagtgactca agacggtatt cagtttgcaa aacagtggta tgaccttaag
     6061 gtaaatgcgg cgcttgtagg agcaatagca gctgctctta aaccggagtt ttatgaacag
     6121 actagtggaa agtgtctgta tgagacatcg gcatatccta acggtggccc tgcaccactt
     6181 actacaaata ctatgagatt ttttgaagaa cataaaaaaa tatattcaat ggctcctggt
     6241 tttatatcct tcccagccta caatgcttta tacgttctta aagacgcact tgaaatggct
     6301 aaggatccca acaataaaga tgatgttata aaatcacttg aaaaagtgta tttcccaatg
     6361 ggtggtcaaa aaggtgttgc tcctgttgta tttgatgagt tccatgatct cagatatggt
     6421 ggggaagacg gagctacagg tattatcttt caatggcaaa agaacggtgc atggaaagca
     6481 gtttatcctg agaaatataa aacaggtgac tgggaaaagc cggaatggat taagtggtaa
     6541 actaacaaat agacggagaa tattctccgt ctatttgttt acttcgagga ttattcttaa
     6601 tatttctcct acagaccaag cctgtgaaaa gcatcctttt ttttcgtgag gccagtttcc
     6661 ctctatgatt tctggaacgg ttccacacca gtcggtattt atcatatctt caatcggaga
     6721 gagcagcttt tttactataa tttttgcttc ttttgaaaag tcatttatct tcaagaaagc
     6781 atacaggaaa tgtcccagaa gataaggcca agcatttccc tgatgataag caccatcacg
     6841 tgtccatctg tcgccaacat atatgccaat atatcttgga tctttgggtg aaagggttcg
     6901 cagaccatat gggatataaa gttcttctaa aaccttatca ataatcagtc tttctttttc
     6961 tctcgaaaga atatcaaatg ggagactgac agcaaatatc tgatttggtc tgaagctaaa
     7021 gtcctttcct tcttcgttta taacgtcgta aagacagtta tcttttgaat tccagaattt
     7081 ttcattaaag ctagacttta ccttttcaat aaggtactca tactcaaact cagcattcaa
     7141 tatttttgaa aaataggtca ttatatttaa agcattaaac catagagcgt taacttcaac
     7201 agtttttcca tatctcggag ttacagacca atcttcaact ttcacatcca tccatgtgag
     7261 ctgagtattt ttatttcctg catatataag atagtcatca tccatatgta tgttataatc
     7321 tgttcctgaa atatgttttt ttataatttc atataagtat ggatacattt tcttcaaaaa
     7381 tatatcatta tgtgtaaacg tatagtattt atataatgca taaaaaagcc agagggtggc
     7441 atcagcagta ttgtatcctt tgggttgtcc agaataatca tcaaatacat ttggaataag
     7501 tccattttga agattattta caaaattctc tattacttct ttagcaatag agtaattcct
     7561 agtatagagc aatagacctt caagagcaat catggtatct ctgccccaat cagtgaacca
     7621 tggaaagcct gcgattatag tatgttttcc agtacttttc cttttcatta taaaggtatc
     7681 ggctgcaata agcaatcgtt ttatggtatt atcagaagaa gaacttgaaa tactaataat
     7741 tctttccttt tcagaagtga gggcagtaaa gggatcaaca tcacttacat catcgttggc
     7801 accaaagatt atataaaagc tttcctgtgg atgcagtttt tttgttatac gtaccttgga
     7861 aataatttcc tcatatgcaa aaagtccccg ttcattttct gcatcataga aaacgttgtt
     7921 taaagtatgt gaatactctt ggatctctcc atcagttttc aaagatatgg agttaagaaa
     7981 gtttgttcca aataaaacaa aattctgaga aaactttaag atatttattc taatgcttcc
     8041 aggaagagta ttagagtgaa catcgcggtc attaaaatat ggagttatag taagagttag
     8101 ctcttttcca aagatatttt tatagaacag aacactcata tttctatttt ttatcataaa
     8161 gatacttttt tctattatat ttgaacctgt cttgaattcc catacaggaa atccttcata
     8221 cttgaacgat aatatatttt cataaccgct ttctaaaaaa ccgttaacag tctttaaagt
     8281 acttaatata tatgttgaat cttctacaac agctatttct gaggctcgtg atacaaatat
     8341 ccttctgtta actggaggat tgagtgctgc ggaaagtata gaatggtatc ttctggtgtt
     8401 cagtccgcta gcgcatgccg aagaacttcc accaagtcca tttgcgataa ggtactccat
     8461 ttcagagtaa tttttcagaa ttgacactat atcacctctt tgatataatc tgattataag
     8521 cagacttaag gaggaaatag atgtttgacg aatataaaga tagtgtgatt gaaggatgta
     8581 aaagaatagt agctgaaaat ttagtaaaag gaacatgggg aaacgtttct gtcagtgatg
     8641 gcgaaagaat ctttattact ccctcgaact ttccatatga aaagcttaca cgtaaagaca
     8701 tagcagttac ggacatgaag gggcagatag tactttcaga gcataaacct tcttctgaac
     8761 tcaaaatgca tattgcactt tacaatgcca gaaaggatat aaaagcagta attcatactc
     8821 atccagtttt ttcaagtgct gtttcactgg tgtgtgaaaa aatcccttct cttatagaag
     8881 attcagttat gatctgtggt gaagaaattc tggttgcaca gtttgcgtca gccggttcag
     8941 aagaacttgc gtataatgtt gtaacggcta tgggaaagaa caatgcaaca attatgaaga
     9001 atcacggttt agttaccata ggtgattcta tcaacgaagc aattacagca tcgcttgtct
     9061 gtgaaaaaac tgctcagatc tatatagttg ctctttcatg tggaaatgtt tcggttattt
     9121 ctcccgagca agccacacag ttaaggcaga aatatcttaa aaattacaaa cagtgagtca
     9181 tgatgtcttt atatggcctg tggaatcgat tttaatcttt cggttgctga atatttcaag
     9241 tacttccctt tgagtacagt ttgttagtga tgtaaactca ttcagagtta ttcctacggt
     9301 tataaatttt tccaggtatg tatcaattat gcttgtaata cgttctctct tattggtaat
     9361 acaaactgtt ttaaaaagct catccaaaaa agttattctg cttatttgac attttaccat
     9421 cttcataccc aatgcgggaa actgcgaaat aactgatttt aacttttcac ctgacatttt
     9481 caccacatca cagtaaacgt cggtatatac tgttgccata cggcagttgg aattaaacat
     9541 ccctgcttcg ccaaagaaat cgcctttttg gattatagaa agaagaactt ctttttttcc
     9601 agaatagcgg ccgactttca ttccaccgtc caataaaaga tatccttcat tatttggatt
     9661 ttcaccttct gctatcagaa tagtattagg cggaacatgt atcccatatt tttcgtataa
     9721 actcaaaaga gtgtcgtttt tgttttcaag aagattttta agatatgtaa aagataattt
     9781 tttagagtat tttgcatgat caaatgtgtt gtaaaggtgt ttggctttct cgggatctaa
     9841 atgaaaggtg attatggcct tccaaatatc aagttcatca cggagcggat agtctatata
     9901 gtcggatttg aaagcatata cttcgtttaa agcctgctga tagttttcct gattgtaaag
     9961 gaacttcatt ttgctgaaaa agtctagaaa ttttgattct gttcttaact tgtgatgagg
    10021 atgaaatctt ttaaaagtat taattgcact ctcctgatca atctgactgc ttatgcattc
    10081 atcaaggaag gtaagccttt ttgtaagaga ttcaaacatt tcaaaataaa tattttcttt
    10141 agagtcaagc gaattaagat cctcgggggt tagtatctga atctggcaat cagttaaagt
    10201 atatgcagaa tcatagcatt ttgcaccggc tattatccac tctccaaagg ttccagtttc
    10261 aataatattt ttatttccgt tgtgagaaat ctctatttta ccagatacaa gtctataaat
    10321 attttccgag atacttccgg cctcgtaaat gtactgtcct ttctttactg taatgctttc
    10381 catctttttc acctcaaacg taagtattat cattacaagt atatcatgat aattatactt
    10441 aggcataagg aggcttgcta tgcttaataa agaagaagca acagagcttt tatatcagaa
    10501 cataagtaca gaaacactta taaaacattc aagagccgtt ggagcaataa tgaaacatct
    10561 ggcagttttt ttaggcttcg atcctgtcaa atgggaagtt acaggtctta ttcatgatct
    10621 ggactatgaa ctcactaagc ataatgttga tcaacatgct ctaaagacag tggaaatgct
    10681 taagggagct attgatgagg aaatggagaa cgcagtactt gcccataatg ataaaaaagt
    10741 tcttaatgct cctatagaaa ttgcattata cgcagctgac cctctttcag gatttataac
    10801 agctgcggta cttgttattc cttctcaaga catccgtgat cttaaggtat cttctttaaa
    10861 gaagaagttt aaagataaaa gttttgcaaa aggtgcaagt cgtgaaaata ttttaaagat
    10921 tgaacagttg ggcataacct tggaaagttt ttttgagata gctttaaagg cctttaaaga
    10981 ggaaagttta taaaggtact tgacaatcat aaaccaaatg actataatta tagtcaaggg
    11041 gcgaagaatg aatcttaaag atcttgaatt ttacagtctt gcagaagcaa aagctaagtt
    11101 ttcaaaagtt atggacgaaa caaagaaaaa aaatattgtt attaccaaaa acggacttcc
    11161 tcaggccata attgttgatt atgataagtt tgtaagcctt atggagtttg ttgaccagat
    11221 aaaagattta tatcttgtgg aagctgggga tctttcaaga gtaaaagaaa tagaaaaatt
    11281 ttttttagat tttaacgaag aatagtatgg aggtgtagta tggcagacgt tattcttgaa
    11341 catatcgata aggtgtatcc taatggtttt aaagctgtaa aagacgctaa catagaaatt
    11401 aaggacagag agtttgtagt tcttctcgga ccttctggat gcggcaaaac caccacactg
    11461 cgaatgatag caggtcttga ggatatttca gcaggaacta taaagatagg cgataagatt
    11521 gttaacgata ttgaaccaaa agacagagat atagcaatgg ttttccagaa ttatgctctt
    11581 tatcctcata tgtctatata cgataacatg gcttttggac taaaattaag aaaaatgcct
    11641 aaagatgaga tagaaaaaag agttaaagaa gccgcacgaa tacttgatat agagcatctt
    11701 ttagacagaa agcctaaaca gctttctggt ggacagagac agagagtcgc agtaggaaga
    11761 gctattgtaa gaaaccccaa agtcttcctt tttgacgaac ctctttcaaa ccttgatgcc
    11821 aaattaagag tccaaatgag aagcgaactg aaaaaactcc actcaagatt gaatgccacg
    11881 attgtttatg ttacccacga tcaggttgaa gctatgacta tggcagataa aattgttatt
    11941 atgaaagatg gaattatcca tcagatagga gatccgttcg aagtatacca taaaccgagc
    12001 aatatgtttg ttgctggatt tataggtaca cctgcaatga actttcttaa tgcgaaattg
    12061 agcactcaga atggttccat gtggctcact gctgagaata tgaacataaa agtcccgaaa
    12121 aagtttgaag atatccttaa gccttatatt gataaagaag ttgtatttgg tataagacct
    12181 gaagatgtat acgataagtt atatgcagtt gctgctaccg ataataacac tgttacttca
    12241 actgttgatg tggttgagcc tttgggaagt gaaacacttt tacatcttac tattggtaag
    12301 caggtaataa cagccaaggt tgatccaaaa tccatggcaa aatctgatca gaagcttgat
    12361 atcgtttttg atatggagaa aatgcatatc tttgataaag attctcagca ggcaattttt
    12421 taataggtag atatatattc tgtattaaaa aatcatggtt tctagatatt cagaaaccat
    12481 gattttttct tagctacgat caaaagattt ctttttagtt aacttaaaac ataattagaa
    12541 ttaaataaat ttcgttttac atacaaatat tatagaaatt atgatataat atttaagtgt
    12601 attcattata ttttaaaggt atatcaaggg ggatatatta tgatattaaa aatggtattt
    12661 gaacctgcta ctgatgaatt ggttgatctt ccagttcatt ataacaggcc tttgcaagga
    12721 atgttctaca atctgctttc gcaatatttc ggttcttcta taaattcaga tgaaagagaa
    12781 aagaaaatat ttactttttc tagaatctat ccttatgaaa gcttccgagt tgacaataaa
    12841 agacttaagt tcaaaggttc ttttatggta tactttgctt ctcccataga agaattaatg
    12901 gattgtgtat caaaggcttt ttctgaaaaa gagcttttta ggatagaaaa taatctattt
    12961 aggctgaaaa gtcatgttat tcttaaaacc gaagtgaatt cgcccattat ggtgaaaact
    13021 gtttcaccta taaccgttta tagtactata acgcttccaa acaaaaataa gtatacacat
    13081 tacttttcgc cttattcaga caacttcaga aatcttataa gcgaaaatat aaaacgaaaa
    13141 gcttctgttt taggtataga cttttcagaa gataatacgt ttgatatatc tccatacgga
    13201 gtaactgaaa aaaacgagaa gcttctgttt tataagggaa taataataaa aggctggaca
    13261 ggatattttg tgctttcagg aaataagaat cttatccgtc ttgcttttga ttccggatta
    13321 ggagcaaaga actctcaggg atttggtatg attattccgg ctgatcataa tcaggcatat
    13381 gaagccattg ataaaagcga tgattcgatt ttaacagatg attataaacc tcatgatgtg
    13441 tatgaaaaag aggttttttc aaatacagtt atataaatac tattacatac aaaatgtttg
    13501 cctaatatca taaaggagta gataagtatg atcaaaaatg cagtc
//
LOCUS       DAOSHR010000038        13451 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_3827_length_13451_cov_4.941699, whole genome shotgun sequence.
ACCESSION   DAOSHR010000038 DAOSHR010000000
VERSION     DAOSHR010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 13451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13451
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3827_length_13451_cov_4.941699"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /locus_tag="PLU28_03400"
     CDS             complement(<1..508)
                     /locus_tag="PLU28_03400"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOG34143.1"
                     /translation="MILINIDRNLDTPVYQQIVEKMRELIDNGDLRPGEKLPSTRELA
                     EKLGVNRSTVYLAYEELWVTGYIQSNPGSYTFVRRKRTYPLKNEPSVKAAIDWNTCID
                     KRINELYTWDTTSYEKNLTFPREFLDMKSISPDERLYPVKTFKVAFNAAVKENMSKMF
                     SYSDIKGYL"
     gene            650..1300
                     /locus_tag="PLU28_03405"
     CDS             650..1300
                     /locus_tag="PLU28_03405"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOG34144.1"
                     /translation="MKKFLIIILRGGIDIRLLIIRHGQSQADIEKRHEGRADFPLTKL
                     GIKQAGLLSKWIVKNYLPDYILSSTLKRAAQTAELIGNETGVKVESMSELMEFNNGLL
                     AGLLFEEAAAKYPRPSIKNLHDKIYEQESMIEFRSRAEVVLSKIMHEYPHDKTVAVVT
                     HGGMINMLFRSFINLPLVTEVSVNTSDTGVHLWTWEEGKRHIIFMNRTDHLMESGI"
     gene            complement(1350..3056)
                     /locus_tag="PLU28_03410"
     CDS             complement(1350..3056)
                     /locus_tag="PLU28_03410"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HOG34145.1"
                     /translation="MFTDEKLGKRIRKFYPENLEVTSWETVEREFKKLLEEPIDSKEA
                     LMVFMEKFSEFYSIIAQISTEKYIKMTCYSDKPENHEDYNKFFSEIQAPSSKYEFLIN
                     KKFYDSPYRKELGKEYDHMNKIISRKIEAFCEKNIPLDAKENKLSSKYGEIISKLSTE
                     FLGKERTITQLSVYLKDPDRTVRQQAWEKINGLIAGKRDELEELFDSLMEVRVEKAKN
                     LGFSNYRDYKHFTKERFDYTPEDLYEFHSSVEKAVLPILKELFETKRQKLGIDTLRPW
                     DTAADEDGITLKPYKTNDEFVDNAIKILHKVKPEFGKNLELMKNTGFLDLENRKGKAP
                     GGYNTQLPEYGGSFIFMNAVGIHANVITLLHESGHAMHGFSCKHIPYNVYKRYPSEVA
                     ELASMSMELITMDYLDEYYKDRVDIKKAKKEGLMSTLNILPWAMTVDAFQQWIYTTNH
                     TAGQRDAYFAGLMDRFNYGENYSGYEKEKALRWLRQLHIFEVPFYYIEYAMAQLGAIA
                     VFKNYKENGEKAIEQYHNALKLGYSKPIPEIYRTAGIKFDFSYEYIKELVGFIKNEID
                     KL"
     gene            3369..4289
                     /gene="yhfZ"
                     /locus_tag="PLU28_03415"
     CDS             3369..4289
                     /gene="yhfZ"
                     /locus_tag="PLU28_03415"
                     /inference="COORDINATES: protein motif:HMM:NF041241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HOG34146.1"
                     /translation="MALNSELMQKNGLMATLLAREFITKKIDDRINTVSYYAQKYSYG
                     RGTVQNAMGYLKQAGAVELVSRGHMGTFIKSLDYEKLWVLTSWETLMGVMPLPYSRLY
                     EGLATGLYKSAQSRKIPFTLSYMRGAKNRIDALQRDRYDFAVISKFAYIKSIDTAEIE
                     MVMDFGKYSYVNEHAVVFSQDKNEIQDGMIIGIDKSSPDLYYLTLEVCKGKKVIFKEL
                     PYNQILTKLLNKELSAAIWNMDEINDRKIEINTMKIELEGFNMEDTNAVIVVKKGNDG
                     VKNLLLQVLEIKKVAEYQKKVLAGKMIPNY"
     gene            4305..4664
                     /locus_tag="PLU28_03420"
     CDS             4305..4664
                     /locus_tag="PLU28_03420"
                     /inference="COORDINATES: protein motif:HMM:NF013070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HOG34147.1"
                     /translation="MDLFLRLNILKDSGQIDQHIFDSISEFISFFKQAHGIALTEENG
                     SMLITHLCIALDRIKKGKTVNSLETDIYEEITGDCNFEKAVKVTDGLERIFGGSIPDD
                     ERGYIYMHLCVLLKQND"
     gene            4680..5039
                     /locus_tag="PLU28_03425"
     CDS             4680..5039
                     /locus_tag="PLU28_03425"
                     /inference="COORDINATES: protein motif:HMM:NF022394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HOG34148.1"
                     /translation="MLNIVVGGQIDKDMVARKIREIGKEKVSVVIMSDIQAAMAIKAG
                     NADYYFGACNTGGGGALAMAIALLGMNLCATVSMPGKIKSDAEIQNEVKNGKKAFGFT
                     PQHADQVLPVIMKEILK"
     gene            5058..5594
                     /locus_tag="PLU28_03430"
     CDS             5058..5594
                     /locus_tag="PLU28_03430"
                     /inference="COORDINATES: protein motif:HMM:NF022254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HOG34149.1"
                     /translation="MGYVLIALIGCMAAILANKGVAVFNDGLRPIMPEYLEGRMDKKA
                     LAATSFALSFGLVIGFGIPVSIAATIILIHSVLLGTDIIGTWSPSGKKGMIIAGVVGA
                     LYGLGIMVGLQVIVDVFAKLPVNFLSNLGKVGDPIIAAFAVFPAIVVGYQYGVKHGLF
                     HTGSLFHCETDSSVLWSV"
     gene            5527..6339
                     /locus_tag="PLU28_03435"
     CDS             5527..6339
                     /locus_tag="PLU28_03435"
                     /inference="COORDINATES: protein motif:HMM:NF022254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HOG34150.1"
                     /translation="MVFFTLVVSFIVRQIVQYYGVFKVGTSSIALTPEGMALLAGMII
                     MIIYAMREKAEPDSKGIDYKGLFGDRVSRIKKNIVWLSIMGGLVAAATSYGILAGDPI
                     SLNLLKDGKNAEAALTAFARGIGFIPLVATTALATGVYGPVGMTFVFVVGILTKNALL
                     AFILGAAVIVLEVFLLGALAKLLDKFPGMRKAGDNIRTAMSKVLEVALLVGGMIAANA
                     MAPKLGFFVVVGLYVLNKVSKKPIVDMAVGPVGAIIVGILINILSVIGLYKV"
     gene            6386..7471
                     /locus_tag="PLU28_03440"
     CDS             6386..7471
                     /locus_tag="PLU28_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34151.1"
                     /translation="MDKREELIYSTYKKQLRAFEFLGTTIEKINYDVPDILTEKMESW
                     ERIYLMGVKNSSGIDERAVKKLSQANFIFNTLDKMSDGGRAIDVNLRNPVSGGIMTGS
                     SSAGAVNVLYGINDIALATDGGGSVLGPAFSLNLFSCLLKGLGYQSQSKKKSTDGIEF
                     CAGTGLISYDYGIMKEAVKVLTGEVDSYTGKITVAVPCNLISPDGKSHMELLSGVLDG
                     IENLKIIEYEFPDFMNRENSIKAMKEIFADADVVACAEGPIDTLGIGDSVYGSFSDTK
                     ASQARSGKNLLRIANMLNCSAIGIPCDVFSKGILLCGPSGTAVFSKLITLADHVAYKF
                     MQSNLVHEYFHRSFLRRHNEYTFKDGD"
     assembly_gap    7548..7647
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7648..8513
                     /locus_tag="PLU28_03445"
     CDS             <7648..8513
                     /locus_tag="PLU28_03445"
                     /inference="COORDINATES: protein motif:HMM:NF014212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34152.1"
                     /translation="YMHEHMKLDLSGIKKDEDCRLDCYEQTKSEVSELKEKGVSNIVE
                     VTNIGMGRDISYVIKLMEETGMNFIFSTGFYKDPFLPKYVMDKSEKELSKIMEGEILR
                     GIDGLGAHAGIIGEVGTSLDTITPSELKVFKAACIAHNNTGVPITTHTTLGSMAIEQI
                     EIFKEYGTNLDKIIIGHTDLKGDAQYIKKIINLGVYVEIDTIGKENYLSDEKRLEMVH
                     YLCSEGYSERMVLSMDITRKSHMKAFGGKGYSYLLDFFVPQLRKAGTREEDIQNMLKN
                     NPLKIFGSDVL"
     gene            8510..9622
                     /locus_tag="PLU28_03450"
     CDS             8510..9622
                     /locus_tag="PLU28_03450"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOG34153.1"
                     /translation="MIFNTYPLESLTVEQAKELQFRMIECITRNFTGKEILNRGDLGV
                     SLGINMPHTTQQAEKAISDFFAVESAILVRGAGTGAIRCAMISCLNPSDTVLIHDAPV
                     YPTTGVTIKSMGLNTVCVDFNKIEDIKEKLHCNPQISAVIIQHTRQKIDDRYSLEQVI
                     RCIKDEKPELTVIVDDNYAVMKTPQIGVQCGADLSAFSAFKLLGPEGIGCIIGKKEFI
                     DKIRAMNYSGGGQVQGHEALDALSGMIYAPVSLAIQAEVIQEVFEYINSGKIKGVKKA
                     YIANAQSKVLLVEFYEDIAAKILVLAQELGAIPNPVGSESKYEFCPLFYRISGTFRQA
                     DPLLEKRMIRINPMRSGAKTIIRILEQSVSSVMSVR"
     gene            9627..10784
                     /locus_tag="PLU28_03455"
     CDS             9627..10784
                     /locus_tag="PLU28_03455"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HOG34154.1"
                     /translation="MFLEKTIKRNPKLIEAAFELHRSGQISPDTYLIDINTLTANAQK
                     IKKEADKYGVKLYFMTKQLGRNPLIASKISEIGYEGAVTVDFREAQNLIENNIKIGNV
                     GHLVQIPSNSIKKILMADPLIVTVYSYDKIKEIDMQACALGKIQEIMLRIYDDKDEIY
                     PSQNAGFRLQELTELISSIKKLKNVKLAGLTSFPCFIYDEKQRDIMPTHNVLTLKKAE
                     EICSKNGIPITQINMPSATCVRTIQKIADLGGTHGEPGHGLTGSTPMHRDFDLEELPA
                     VVYVSEVSHNFEASAYIFGGGHYRRSHMENAIVGRSMTDSIQDKVITPDPQSIDYHIQ
                     LQHSHEVGKCVVMSFRSQIFVTRSHVAVVDLSGGQINLLGVYDSQGKIIGR"
     gene            10789..12012
                     /locus_tag="PLU28_03460"
     CDS             10789..12012
                     /locus_tag="PLU28_03460"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF009049.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HOG34155.1"
                     /translation="MGKFIVIVLDSFGVGFMEDVPRIRPQDNGANTALHIFEKVSGLK
                     LSGLEKLGLMNALNHETDVMKKNPMAVWGRSALMHFNADTFYGHQEIMGTKPRRPVME
                     PFSKSIDAVSEKLIRNGHSVDFIGQNNLRVLLVDGCVTIGDNLEADGGQAYNVTATFD
                     KIAFEKEVEIARIVREEVHVSRVIVFGGKNVPVEKIIGAYEIKDWIYAGINAPGSGVY
                     DEGYFVQHLGYGIDSRVQVPCLLGKKGIKTVLIGKVADIVENPYGESIPGVDTAYVME
                     KTIEKAMEMETGFICTNVQETDLAGHKEDSFLYADRLSVADVYISRLMPMLNPQDIMI
                     IMADHGNDPTIGHSHHTRENVPILIYKEGVQNIFIGERKTLSDIGATVAEYFNAEKPE
                     NGESFLKLISKKEEC"
     gene            12015..12275
                     /locus_tag="PLU28_03465"
     CDS             12015..12275
                     /locus_tag="PLU28_03465"
                     /inference="COORDINATES: protein motif:HMM:TIGR01003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HOG34156.1"
                     /translation="MKTMDILLQNETGLHARPASEFVKLAKSFEAKITLVKDGKEYNG
                     KSILNVLGMGAGKGTKIELRAEGEDEETALEKLSELVKSFSE"
     gene            12284..>13451
                     /gene="ptsP"
                     /locus_tag="PLU28_03470"
     CDS             12284..>13451
                     /gene="ptsP"
                     /locus_tag="PLU28_03470"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HOG34157.1"
                     /translation="MKGTAVSPGIAIGKVFLLKKIEVESLQKNSDDPQKELAILETAL
                     KKSALELEKLIQASGEKLGETDAGIFQAHLMILQDDEFVQTVRDHIQAEKLCAAYAVV
                     KTAEDFYIQFMQIEDEYIRERAADVKDVGMRVIKSILGTGTGLEAMPDGSVIVAQELS
                     PSDTASMDSQKVCGIITESGGRTSHAAIIARNLGIPAIVGAGGITEQVETGTFIVFNG
                     ESGEIFIQPDGELVKKYIEMKAVKEKHKKLLMQFKDKKTFTHDNHQVELYANIASQND
                     TSAALENGAQGVGLFRTEFLYMNRDNEPSEEEQFVIYKKVLSDMKSKPVIIRTLDIGG
                     DKNVPYLGLEKEDNPFLGFRAIRICLEKKEMFKKQLRAILRASVFGRARIMFPMI"
BASE COUNT         4275 a         2335 c         2958 g         3783 t
ORIGIN      
        1 gcaggtatcc ttttatatcc gaataggaaa acattttgga catattttct tttactgcag
       61 cattgaaagc tactttaaaa gtcttaacag gataaagccg ctcatcgggc gatatggatt
      121 tcatatccag aaactcccta ggaaaggtaa gattcttttc gtagctggtg gtatcccatg
      181 tataaagctc attaattctc ttatcaatac aggtgttcca gtctattgcg gcttttactg
      241 aaggttcgtt cttcaatgga tatgttcttt ttcttcggac aaaggtatat gagccgggat
      301 tgctttgtat atagcctgta acccaaagct cttcatatgc aagataaacc gtggagcggt
      361 ttactccaag tttttcagcc agttcgcgtg tggagggaag cttttccccc ggtctgagat
      421 caccgttatc tataagttca cgcatttttt caacaatctg ttggtataca ggagtgtcaa
      481 ggtttcggtc tatatttatt agtatcatca gatccatcct cttttgattt tatgatacta
      541 ttatagaaca aaactttctt tatgaaaaca gccggttgat atattttttt accaaccggt
      601 tttaacatgt tagttacgta aaaaccttga cattatggca tataatttat tgaaaaagtt
      661 tttaattatt atcctacggg gagggattga tatcaggctg cttattatac gtcatggaca
      721 gtctcaggcg gatatagaaa aacgccacga aggaagagct gattttccgc ttaccaagct
      781 tggaattaaa caggcaggac tgctttcaaa gtggatagta aaaaattatt taccagacta
      841 tattttaagc agtacgctga aaagagcggc acagacagcc gagcttatag gaaacgaaac
      901 aggtgtaaag gtagaaagca tgagcgagtt gatggagttt aacaacggtc tgctggcagg
      961 actgcttttt gaagaggctg ccgcaaagta tccgcgtcct tcaataaaaa acctgcacga
     1021 taaaatatat gagcaggagt ctatgataga gttcaggagc cgtgccgaag tagtcctttc
     1081 aaaaatcatg catgaatatc cgcacgataa aacagtagct gttgttactc acgggggaat
     1141 gataaacatg cttttcagga gtttcattaa tctgccgttg gtaacggaag tatctgttaa
     1201 tacgtcggat acgggggttc atctttggac atgggaagaa ggaaaaaggc atataatctt
     1261 tatgaaccgt acggatcatc ttatggaatc aggtatatag aaataataaa ccggtcagct
     1321 taatgctgac cggttttggt aagagataat taaagcttat ctatttcatt tttaataaaa
     1381 cctaccagtt cttttatata ctcatatgag aaatcaaact taattcctgc ggttcggtat
     1441 atctctggaa ttggcttgga atatcccagc ttcaaggcgt tatgatactg ttcgatggct
     1501 ttttctccgt tttccttata atttttgaaa acagctattg ctccaagctg agccattgca
     1561 tactctatat agtagaaggg aacttcaaag atatgaagct gtctgagcca ccttaaagct
     1621 ttttcttttt cgtagccgct gtaattttct ccgtaattga atctgtccat aagaccggcg
     1681 aagtatgcat ctctttgacc ggcagtgtga tttgtggtgt atatccactg ctggaatgca
     1741 tcaaccgtca tggcccatgg cagaatattc agagtactca tcagtccttc tttctttgcc
     1801 ttttttatat ctacgcggtc tttatagtat tcgtcaagat agtccatggt tatcagttcc
     1861 attgacatgg atgcaagctc agccacttca gagggatatc ttttataaac attgtatgga
     1921 atatgcttac atgaaaaacc gtgcatagcg tgacccgatt catggagcag agttatgacg
     1981 tttgcatgta ttcctactgc attcatgaat ataaaagatc caccgtactc cggaagctgg
     2041 gtgttataac ctcccggagc ttttcctttt cggttttcaa gatccagaaa acctgtattt
     2101 ttcatcaatt caagattttt tccaaactcc ggctttacct tgtgcagtat ttttatagcg
     2161 ttatcaacaa actcatcgtt ggttttataa ggcttaagag ttataccgtc ttcatcggct
     2221 gctgtgtccc acggacgtaa agtatcgatt ccaagctttt ggcgcttggt ttcaaaaagc
     2281 tcttttaaaa tcggaaggac agccttttca accgaggaat gaaattcata aagatcctca
     2341 ggagtgtaat caaatctttc cttggtaaag tgcttataat cacggtaatt tgaaaaacca
     2401 agattttttg ctttttcaac acggacctcc atcagactgt caaacagttc ttcaagctca
     2461 tctcttttac cagcgataag accgtttatt ttttcccatg cctgctgacg cactgttctg
     2521 tccgggtcct taagatagac agacaactgg gttatggttc tttcttttcc caaaaactcc
     2581 gtgcttaatt tggagataat ctccccatac tttgaagaga gtttattttc ctttgcatca
     2641 agcgggatgt ttttttcaca aaaagcctct atctttcttg agatgatctt gttcatatga
     2701 tcgtactctt ttccaagttc ttttctgtaa gggctgtcgt aaaatttctt gtttatcaga
     2761 aactcatact ttgaagaagg agcctgtatc tccgagaaaa acttattgta atcctcgtga
     2821 ttttcaggtt tatcggaata acaagtcatt tttatgtact tttctgttga aatctgagct
     2881 attatcgaat aaaactcact gaatttttcc ataaaaacca taagtgcctc tttagaatct
     2941 ataggctctt caaggagctt tttaaactcc ctttcaactg tttcccacga agttacttca
     3001 aggttttcag gataaaactt cctgatccgt tttcctaatt tttcatctgt aaacaaagta
     3061 atccctcccc ttttaaagta ggtaagtgaa tctttctgtt tcaattataa catactggat
     3121 ttcataaaat atccatatat atatattggt tggtctaccc aggttgatag ttgctaaaaa
     3181 atacacaatt tggaagtttt atgagttaaa tgctgataat ctcatcttac aatcaaaaaa
     3241 aagccgaaaa aaggcttttt tttgattgta actttggtta taatacaagt aatatacaaa
     3301 agattgtaca ttgatcttta ttttttaaac agacaaaata caaaattctg tacgatgagg
     3361 attataatat ggctttgaac agcgaattga tgcaaaaaaa tggtttgatg gcaacacttt
     3421 tggcaagaga gtttataacc aaaaaaattg atgacagaat aaacaccgtg tcttattatg
     3481 cccagaagta ttcctatggc agaggtacgg ttcagaatgc catgggttat cttaagcaag
     3541 ccggagctgt tgaacttgtt tcacgcggac atatgggaac ttttataaaa tcactggatt
     3601 atgaaaagct atgggtgctt acctcttggg aaacgcttat gggagttatg ccgcttcctt
     3661 attcgagact ttatgaagga cttgcaaccg gtctttataa aagtgcccaa tcgaggaaga
     3721 taccgttcac actttcttat atgcggggag caaaaaacag aatagatgca cttcaaagag
     3781 acaggtacga ctttgctgtc atatccaagt tcgcatatat caagagcatt gatacggcag
     3841 agatagaaat ggtaatggat tttggcaaat actcatatgt aaacgaacac gcagttgttt
     3901 tttcgcagga taaaaatgag atacaggacg gtatgataat cggtattgat aaatcttcgc
     3961 ctgaccttta ctatcttacg cttgaggtct gcaagggaaa gaaagtaatt tttaaagaac
     4021 tgccgtataa tcagatactt acaaaacttc tgaataaaga actttcagcc gcaatatgga
     4081 atatggatga aataaacgat aggaagatag aaataaatac tatgaaaata gagcttgaag
     4141 gctttaatat ggaagatacc aatgcggtta ttgttgtaaa aaagggaaac gacggggtta
     4201 aaaatctcct gcttcaggtt cttgagataa aaaaggttgc agagtaccag aaaaaagttc
     4261 ttgcaggtaa gatgatacct aactactgat tttcggaggc aatgatggat ttatttctaa
     4321 gactcaatat tcttaaggat tcaggacaga tagaccagca tatttttgat agtatatccg
     4381 agtttatatc ttttttcaaa caggctcacg gcattgctct tacagaagaa aatggttcaa
     4441 tgcttataac tcatctttgc attgcgctgg acagaataaa aaaaggaaag acggtaaaca
     4501 gccttgaaac agatatctat gaggaaatta ccggtgactg taattttgag aaagcagtaa
     4561 aggtaactga cggattggaa aggatttttg gcggttcaat accggatgat gaaaggggtt
     4621 atatatacat gcacctttgt gtgctattaa aacaaaatga ctagaaagga gctgtcaagt
     4681 tgcttaatat cgttgtaggc ggccagattg acaaggatat ggttgccaga aagataaggg
     4741 aaatcggaaa ggaaaaagtt tcggtagtca tcatgtcgga tattcaggcg gcaatggcta
     4801 taaaagccgg aaatgccgat tattatttcg gtgcatgtaa taccggtggt ggcggagcgc
     4861 ttgccatggc tattgcattg ctcggtatga acctgtgtgc gacggtttct atgcccggaa
     4921 agattaaaag tgatgcagaa attcaaaacg aggttaaaaa cgggaaaaaa gcgttcggtt
     4981 ttactccgca gcatgccgat caggtacttc ccgttataat gaaagagatt ctaaaataac
     5041 tttagggggg taagtctatg ggatacgtgt taatagctct gatcggatgt atggctgcaa
     5101 ttcttgccaa taagggcgta gcagttttca acgatggatt aaggcctata atgccggagt
     5161 atcttgaagg cagaatggac aaaaaagcac ttgcagcaac cagttttgca ttgagttttg
     5221 gattggttat tggttttgga attccggttt caatagcggc aaccataatt cttattcact
     5281 ctgtgcttct gggaacagat ataataggta catggtctcc ttcaggcaaa aaaggtatga
     5341 taatagccgg tgttgtcgga gcattgtatg gtcttggaat catggtaggt ctgcaggtta
     5401 tagtagatgt ttttgccaag cttcctgtga acttcctttc aaatctcgga aaggttggag
     5461 atcctataat agctgcattt gcagtttttc cggcaatagt tgtaggttat cagtacggtg
     5521 ttaagcatgg tctttttcac actggtagtc tctttcattg tgagacagat agttcagtac
     5581 tatggagtgt ttaaagtagg cacatccagt atagctctca cccccgaagg tatggcactg
     5641 cttgcaggta tgatcatcat gataatctat gccatgagag aaaaagccga gccggattca
     5701 aagggtattg actataaagg actattcgga gacagggtct caagaatcaa gaaaaatatc
     5761 gtatggcttt ctataatggg aggacttgtg gccgccgcga caagttatgg aatacttgca
     5821 ggcgatccta tttcgctcaa tcttcttaaa gatggaaaaa atgctgaagc agcccttact
     5881 gcttttgcca gaggtatcgg ttttattcct ttggttgcaa caaccgctct tgcaacaggg
     5941 gtttacggtc cagtcggaat gacattcgta tttgtggtgg gtatacttac caaaaatgca
     6001 ctgcttgcat ttattctggg tgctgcggtc atcgttcttg aagtatttct tttgggtgcc
     6061 cttgccaagc ttttggataa gttccccggt atgagaaaag caggcgacaa cataagaact
     6121 gctatgagta aagttcttga agtagctctt cttgtcggtg gtatgatagc tgcaaacgca
     6181 atggctccta aactgggatt ctttgtagta gtcggcctgt atgttcttaa taaagtttct
     6241 aagaagccaa ttgttgacat ggcggttgga cctgtgggag caataatagt tggaatactc
     6301 ataaacattc tttcagtaat aggtctgtat aaagtataaa ggaaatggag gctttacgcc
     6361 tccattttta aaataaggtg tgaagatgga taaaagagaa gaacttattt acagcacata
     6421 taagaaacag ctcagggctt ttgagttttt aggaaccacc atagaaaaaa tcaactatga
     6481 tgttccggat atactgacag aaaaaatgga aagctgggaa aggatttacc ttatgggagt
     6541 aaaaaacagt tcaggtattg atgaaagagc cgttaaaaaa ctttcccaag ccaactttat
     6601 atttaatacg ctggacaaga tgtcagacgg tggacgtgcc attgatgtga atctgcgcaa
     6661 tccggtttca ggaggcataa tgaccgggtc ttcatcggca ggtgcggtaa atgttcttta
     6721 cggaataaac gatattgctc tggctacaga cggcggagga tcggttttgg gtcctgcatt
     6781 ttccctgaac cttttttctt gtcttcttaa aggtcttggc tatcagtcac agtctaaaaa
     6841 aaaatctact gacggaatag aattttgtgc aggaacagga ttgatttctt atgattacgg
     6901 cataatgaaa gaagctgtaa aagttctgac gggcgaggtt gattcttata ctggaaaaat
     6961 taccgttgcc gttccatgta atcttatatc gcctgatgga aaaagccata tggagctttt
     7021 gtcaggtgta ctggacggta tagaaaattt aaaaataata gagtatgagt ttcccgattt
     7081 tatgaacagg gaaaactcta taaaagctat gaaagagata tttgccgatg cagatgttgt
     7141 agcatgtgct gaaggcccga ttgatacttt gggcataggt gacagtgttt acggaagttt
     7201 ttcggatact aaggccagtc aggctagatc cggtaaaaat cttcttagga tcgccaacat
     7261 gcttaactgc agtgctatcg gtattccatg tgatgttttt tcaaaaggga ttctactgtg
     7321 tggtccaagc ggaacagcgg ttttttcaaa acttattacc cttgcggatc atgtggcata
     7381 taaatttatg caaagcaacc ttgtgcatga gtactttcac agatcttttc tgagaagaca
     7441 taacgaatat actttcaagg acggtgactg aaaattataa aaaacggata tacatatatg
     7501 cacgaacata tgaagcttga tctttcaggt ataaaaaagg atgaagannn nnnnnnnnnn
     7561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7621 nnnnnnnnnn nnnnnnnnnn nnnnnnncat atatgcacga acatatgaag cttgatcttt
     7681 caggtataaa aaaggatgaa gactgcaggc ttgactgcta cgagcaaacc aaatccgaag
     7741 tatccgagct taaagaaaaa ggtgtttcaa atatagttga agtgaccaat ataggcatgg
     7801 ggagagatat cagctatgta ataaagctga tggaagaaac aggcatgaac tttattttct
     7861 caaccgggtt ttataaagat ccttttcttc ccaagtacgt tatggacaaa agtgaaaaag
     7921 agctttctaa gataatggag ggtgagattc ttagaggaat agacggattg ggagcacatg
     7981 ccgggataat aggagaggtc ggaacatctc ttgatacaat caccccctcc gaactcaaag
     8041 tttttaaagc agcctgtata gcccataata atacaggagt gcccataact acacacacta
     8101 ctctgggcag catggccata gaacagatag aaatattcaa agagtacgga accaatctgg
     8161 ataagataat aataggacat acagatttaa agggagacgc acagtatata aaaaagataa
     8221 tcaatctggg agtttacgta gagatagaca caataggaaa agaaaactat ctcagtgatg
     8281 aaaagcgttt ggaaatggtt cactaccttt gcagcgaggg atattccgaa aggatggtgc
     8341 tttcaatgga cataacaaga aagtcacata tgaaggcttt cgggggcaag ggatacagtt
     8401 atcttcttga tttttttgtt cctcagctca gaaaggccgg aaccagagag gaagatattc
     8461 agaatatgct taaaaacaat ccattgaaaa tttttgggag tgatgtgttg tgatatttaa
     8521 tacttatcct ttggaatcct tgacagttga acaggcaaaa gaactccagt tcaggatgat
     8581 agagtgcata acccgcaatt ttacgggaaa agaaatatta aacaggggcg atcttggagt
     8641 ttctttaggt attaatatgc ctcataccac ccaacaagca gaaaaagcca taagtgattt
     8701 ttttgctgta gagtcggcaa ttcttgtaag aggtgcagga acaggagcca taagatgtgc
     8761 catgatttcc tgtcttaacc catcagatac agtgcttatt cacgatgccc cggtttatcc
     8821 aaccacaggc gtaacaataa aaagtatggg acttaatacc gtctgtgttg attttaacaa
     8881 aattgaggac ataaaagaaa aattacactg taatccccag ataagtgctg tgataatcca
     8941 gcatacacgg cagaagattg atgacagata cagtctggag caggttatcc gctgtataaa
     9001 agatgaaaaa cctgagctta cagttattgt agatgacaac tatgccgtca tgaagactcc
     9061 tcaaataggt gtacagtgcg gagcagatct ttcagctttt tcagcattca aacttcttgg
     9121 acctgaagga ataggatgta taatcgggaa aaaagagttt atagacaaaa taagagcaat
     9181 gaactattcg ggaggcggtc aggttcaggg tcatgaagca ttggacgctc tcagcggcat
     9241 gatctatgcc ccggtaagtc ttgccataca ggcagaggta attcaggagg tttttgagta
     9301 tataaactcc ggaaaaatca agggagtaaa aaaagcttac attgccaatg cacagtctaa
     9361 agtgcttttg gtcgaatttt acgaagatat cgcagcaaag attcttgtac ttgcccagga
     9421 gctgggtgcg attccaaatc ctgtcggttc agagtcaaag tatgagtttt gtcctctttt
     9481 ttacaggatt tccggtacat tcagacaggc agatcctctg cttgaaaaaa gaatgataag
     9541 aataaaccct atgagaagcg gtgcgaaaac aatcataaga atactcgaac agtcggtttc
     9601 ttccgtaatg agtgtgaggt gaagccatgt ttttggaaaa gaccataaaa agaaacccaa
     9661 aacttataga ggctgctttt gagcttcacc ggagcggtca gatatcgcct gacacctatt
     9721 taatagatat caacacttta accgccaatg cacaaaagat aaaaaaagaa gctgataaat
     9781 acggagtaaa gctttacttt atgaccaagc agcttggaag aaatccttta attgcatcaa
     9841 aaatttcaga gataggttac gagggagcag ttacagttga tttcagggaa gctcagaatc
     9901 ttattgaaaa taacataaag ataggaaatg tcggacatct tgtacagatt cccagcaaca
     9961 gcataaaaaa gattcttatg gcagacccct tgatagttac agtttattca tacgataaga
    10021 taaaagaaat tgacatgcag gcttgtgcac tgggaaaaat acaggaaata atgctgcgca
    10081 tatatgacga taaggacgag atatatcctt cacaaaacgc cggcttcagg ctccaagagc
    10141 ttacagagct tataagtagt ataaaaaaac ttaaaaatgt aaagcttgcc ggtctgacct
    10201 cttttccatg tttcatatat gatgaaaagc agagagatat tatgcctacg cacaatgttc
    10261 ttacattgaa aaaagcagaa gaaatttgca gtaaaaacgg tattccaatc acccagataa
    10321 atatgccgtc cgctacctgt gtcaggacaa tccaaaagat agcagatctg ggtggaacac
    10381 atggagaacc cgggcacgga cttaccggaa gtacgcctat gcacagggat tttgaccttg
    10441 aagaacttcc tgctgtggtt tatgtttccg aagtatcgca taactttgaa gcaagcgcat
    10501 atatttttgg tggaggacat taccggcgtt cacacatgga gaatgcaatt gttggcagaa
    10561 gtatgactga cagcattcag gataaagtca taacgcctga tccgcagagc atagactatc
    10621 atatacagct gcagcacagt catgaagtag gcaagtgtgt ggttatgtct ttcagatcac
    10681 agatctttgt taccagaagc catgttgcag tggttgactt atcgggcgga cagataaatt
    10741 tgctgggagt ctatgacagc cagggaaaaa taatcgggag atgagttaat gggaaagttt
    10801 atagttatag tacttgacag cttcggagta ggcttcatgg aagatgtacc aagaataaga
    10861 cctcaagata acggtgcaaa tacagcttta catatattcg aaaaagtaag cgggcttaaa
    10921 ctcagcgggc tagaaaaact gggactgatg aatgctctga atcatgaaac ggatgtcatg
    10981 aaaaaaaatc ccatggcagt atggggaagg tcggcactta tgcacttcaa tgcagatacc
    11041 ttttatggac atcaggagat aatgggtaca aaacccaggc gtcctgtcat ggaacctttt
    11101 tcaaaatcaa ttgatgcagt aagtgaaaag cttatccgaa acggtcacag tgtagatttt
    11161 ataggtcaaa acaatcttag ggtgctttta gtcgacggct gcgtaacgat aggagataat
    11221 cttgaggcag acggcggaca ggcatacaac gttactgcca cttttgataa gattgcgttt
    11281 gaaaaagaag ttgagattgc cagaatagta agagaagagg ttcatgtatc aagggtaatt
    11341 gtttttggag gaaagaatgt tccggttgaa aaaattatcg gagcatatga gataaaagac
    11401 tggatatatg ccggaataaa tgcacccgga tccggagtat acgatgaagg atacttcgtc
    11461 cagcatcttg gctacggaat tgattcaagg gtacaggttc cctgtctgct gggaaaaaaa
    11521 ggaataaaaa cagttttgat aggcaaagta gccgatattg ttgaaaaccc atacggagaa
    11581 agtattcccg gtgtcgatac agcgtatgta atggaaaaga ccatagagaa agccatggaa
    11641 atggaaaccg gttttatatg taccaatgtt caggaaacag atcttgccgg acataaggaa
    11701 gacagctttc tgtatgccga cagactcagt gttgcagacg tatatataag caggcttatg
    11761 cctatgctca atccacagga catcatgata atcatggcag atcacggaaa tgatccgaca
    11821 ataggtcact cacaccatac aagagaaaat gttccgatac tgatatacaa agagggtgtt
    11881 cagaatattt ttataggtga acgcaaaacc ctttcagata taggagcaac cgttgctgag
    11941 tattttaatg ctgaaaaacc tgaaaacggg gagtcatttc tgaagcttat atcaaaaaaa
    12001 gaggagtgct gactatgaaa acaatggata tacttttaca gaatgaaaca ggtcttcatg
    12061 caaggcctgc atccgagttt gtaaagcttg ccaaaagttt tgaagctaaa ataactttgg
    12121 taaaagacgg aaaagagtac aacggcaaaa gcattctgaa cgttttggga atgggagccg
    12181 gcaaaggtac caaaatagaa ctcagggcag aaggggaaga tgaagaaact gctttggaaa
    12241 aattaagtga actcgtaaag tctttcagcg agtaggtgat gatatgaagg gcacagcagt
    12301 ttcaccgggt atagccatag gaaaggtatt ccttttaaaa aaaattgaag ttgaaagtct
    12361 tcaaaaaaac tctgacgatc cgcagaaaga gcttgcaata ctagaaacag ctcttaaaaa
    12421 aagtgctttg gagctggaaa aactcataca agcttccgga gagaagctcg gagaaaccga
    12481 tgcgggtatt tttcaggctc atctgatgat tttgcaggac gatgagtttg ttcagacggt
    12541 cagagaccat attcaagcag agaaactctg cgctgcctat gctgtcgtga aaacagcaga
    12601 agacttttat atacagttta tgcagataga agacgagtat atacgtgaaa gggctgccga
    12661 tgtgaaagat gtcggaatga gagtaataaa aagtattctt ggaacaggga caggacttga
    12721 agctatgccg gacggttctg taatagttgc ccaggagctt tcaccttcag atacagcatc
    12781 tatggacagt caaaaagtct gcggcataat aacggaaagt ggtggcagaa cctcacatgc
    12841 agccataata gcgagaaatc ttggaattcc tgctatcgta ggtgctgggg ggataacaga
    12901 acaggttgaa acaggaactt ttatagtatt taatggtgaa agcggagaga tctttatcca
    12961 accggatgga gagcttgtaa aaaaatatat tgaaatgaaa gctgtcaagg agaaacacaa
    13021 aaaactgctc atgcagttca aagataaaaa aacttttact catgataatc atcaggttga
    13081 actttatgcg aacattgcct cacagaacga tacctctgcc gcacttgaaa atggtgccca
    13141 gggagtcgga cttttcagga cagagtttct atatatgaac agagacaatg aaccttcgga
    13201 agaagagcag tttgtgatct ataaaaaagt actttcggat atgaaatcaa agccggttat
    13261 aataagaacc cttgatatag gtggagataa aaacgttcca tatctcggac tcgaaaaaga
    13321 ggacaatcct ttccttggat ttcgtgccat aagaatatgt cttgaaaaga aagagatgtt
    13381 taaaaaacag ctaagagcga tactcagggc gagtgttttc ggaagggcaa gaataatgtt
    13441 tcccatgata a
//
LOCUS       DAOSHR010000039        13143 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_3961_length_13143_cov_4.961950, whole genome shotgun sequence.
ACCESSION   DAOSHR010000039 DAOSHR010000000
VERSION     DAOSHR010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 13143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13143
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3961_length_13143_cov_4.961950"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="PLU28_03475"
     CDS             <1..652
                     /locus_tag="PLU28_03475"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOG34158.1"
                     /translation="VVRFFPEYMKAKEILETPDMGSGAVARFYRGGNFPSHGTDNWFD
                     DIKKSGGVFVDLSIHDFDYLRTVFGEPESIIARSVKLSETKECKNNDHGLAVIKFKNG
                     VLAHVEGSWAEPDNMPVGFHTKFEVNTKNGMIHFDATESMTFNLQLKRQQNNYVAARP
                     SEYNPYEQELRVFIDAVQNGSEVKVSGEEALKSLRVALAANISAKEKRPVLIEEVK"
     gene            654..1607
                     /locus_tag="PLU28_03480"
     CDS             654..1607
                     /locus_tag="PLU28_03480"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOG34159.1"
                     /translation="MIKIGILGIAHTHGYSYVRQINKMQDAQVTGLYEENTEFAKRAA
                     AELKLKLYSSPQELLDSCDAVIIASENSKHMKYTEMAALKHKHVLCEKPIAVNISDAE
                     HMIKVCKENNVVLQIAFPVRYAPSVRKAKETIDSGVLGDILAVSSTNHGTMPGSWFVE
                     KELSGGGAVIDHTVHVVDIVRWLLNTEITNVSGFYDKLIYDELKVEDCGLLMLELSNG
                     AFMTLDTSWSRPKAHPYWGDVTIRIVGTQGTLFIDAFDEKIEYYCNDKKNGWMNFDIK
                     FDYNLIREFIDCIKTGRKPLTSGEDGLAALEVVLKAYAAKA"
     gene            1655..2827
                     /locus_tag="PLU28_03485"
     CDS             1655..2827
                     /locus_tag="PLU28_03485"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOG34160.1"
                     /translation="MEYRILDDYRLFSKMAGFSFSVTAKNQKIYEERHQEIFHEKREF
                     LGAFDNKDLAAGLILYPYMMNFRNNLIKTFGVGDVCSRADKRGKGEVRYLLNESVRLM
                     HSRSAGLSVLYPFNYDFYRGSGWEVFTNSAMYLLSPSQLKTLPETDNIKAVDMDFPDK
                     QGIEYYNKKVCGKYNHVIRSEIDWKDQLRMFSDNEIKRGVVQFYKNSQVCGMMTYVYS
                     YIPKEDSTVFTVRTFFYDDFETKNSMFLFIKKLSAQISSVNLLAPNDFILFAHLSNSP
                     VALKKGNHCMIRIIDINALDGIFFDSCDADISVELTDSVIKENNGCFRFIIDNKKLRI
                     SRTENAAISTDIGTFSSVLSGYVGIKDMIDCSKIKIIGKYDGTDFKKECTFITELF"
     gene            2837..4093
                     /locus_tag="PLU28_03490"
     CDS             2837..4093
                     /locus_tag="PLU28_03490"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOG34161.1"
                     /translation="MAQITAVIVGAGNRGSVYARYASEYPQKLKINAMVEKNVGKLEY
                     ASQNYDIDKNYCFSEYTQVLEMGKVADAAIITTLDNAHKEIAIEFIKRGYDILLEKPL
                     AVSLEDSLEILKNAKKYNVRLMTCYVLRYSTFFGKLKELINKGIVGPVLAVDQRENIG
                     YFHMAHSYVRGNWHSTKDSSPIILAKSCHDLDILHWMLEKKCTRVFSMGELKYFRKEN
                     MPCGAAPRCLDCLVEEPCPYSAKKIYLQTDNTGWPVSTITNDFSYSARMEVLRNTDYG
                     KCVYQCENDVLDYQTVDMEFEEGISVSFTLTAFTNDKTRIIHIYGGMGEITGDFEKGE
                     ITVHRFGEQAQMIKVVPPYQTAHAGSDFQMMDRFVQVMTDRTGNFIELDLNSTMESHY
                     MAFAAEKSRNEKLPVDMIKFKKEHNF"
     gene            4284..4964
                     /locus_tag="PLU28_03495"
     CDS             4284..4964
                     /locus_tag="PLU28_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34162.1"
                     /translation="MKKILAVITIVLMGISLLAYPWITPSCNILEEGYVAVDAFNPAW
                     GIRYGLFGFMEAGAGQPEGGTYLKFGFNRILDIPLSFSVGYSNDFISNAYFFTGTELN
                     LDIFRIGFGGMLGQNRHYNTVTSTDEYKLFPSVYGRTSIVFEKKTNIENSFSIEGAMR
                     SDMNLTFQNCMVAFYGKEKIKNLNFLWFKDVDILGGIVFTTSDFAKVDLLKDFQIVLG
                     MSTGWKFF"
     gene            5075..6781
                     /locus_tag="PLU28_03500"
     CDS             5075..6781
                     /locus_tag="PLU28_03500"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOG34163.1"
                     /translation="MKRISILIMVFFTISTAFSNIYDGYLLIKAPVFSKIFIGGLYKG
                     TVPESSEIAFALSPGTYELTLKLEGYLDASKKAVVEPVKSTVVSFFPQKINEFKSTSE
                     TKEASGMSYGTVDIISVPYKNLRIDINGYEIGSSDFYSLALVKGSYEIKISMGEQYLK
                     TSINIEKDKHTVLQADFFKKQIFNLYKIDFELPQGSKLFIDDKELSDTEKSVMILDSR
                     HKLKVENELYEPFEQEVVFDGSGVYKPALIPKASYVAVNCNFNDAQVFIDGNFIGNAP
                     VIFKSSISLENSTVMVVKKGYSEYQKTGIKLGDRDIAYIQAQLVPQGLTDVAGGAFRM
                     GDMDYPDIRPVLEVFLGYDFYINPYETTFQEYDEFCNQTSRVRPSDNGWGRDKRPVIN
                     VSWWDAVAYCNWLSEKEGLPKAYDLWGNLLDEKGKITSDIKKVFGYRLPTQAEWEYAA
                     KGGQMSMAFIYSGSDNPESCSWFRKNSLGMTHETGKKSPNELGLYDMSGNVREWCTDR
                     YNSYSLLEKDDPYENEGLGRVVKGGSFEMNELFLRISNRWLKDQEYKDFSTGFRVVKT
                     IQ"
     gene            6938..7762
                     /locus_tag="PLU28_03505"
     CDS             6938..7762
                     /locus_tag="PLU28_03505"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HOG34164.1"
                     /translation="MMNSVIKGFLIAAGAIITVSLLLNLTSSSKLQSTSREIRESFET
                     ISVETISSDIDLSGSDSSRLLYSHDKNIFENHIGLEGKRLFIKETKKDIFRGITSQKG
                     SYAVFLAVYPQSAVLQSMSGNVSISDIKTTEVSITNTSGDIHAVNVNFENFLSSSVSG
                     GVKVQKASVKKLKIKTVSGEVGIEDVASDSIEVEVVSGDISMQGLRQACDIIVKTTSG
                     DVRIKGIDLQGYNVEFSSISGEVFIKDEVSSKGKYRYGVGDKKIKVESVSGDLIIE"
     gene            7841..8740
                     /locus_tag="PLU28_03510"
     CDS             7841..8740
                     /locus_tag="PLU28_03510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOG34165.1"
                     /translation="MLSDKKNFFTPDILERMLQDKKAGIYALDTKRVITFWNEGAQKI
                     TGYESSEILGLSCMDNILNHIDSKGVRLCEKNCPASRAMQDGLPKRAEIFLHHKNGYR
                     IPVVATVYPVKNYDGVVSGVIEVFNDLSEKDRQSQSLVYDELTGILCKQEVEQKIKQA
                     EEDFKKVSVPFGLICFGIDNLSQIAEKYKERVAEKILIMAAKNLSANLRITDETGRWG
                     NDSFMLIVKFVDREQLHDIAQNMLITMNNCFLKEENEIIMASVSAGGSVFKDNDSIYT
                     ITERAVHNLEKARLTDKGSLFID"
     gene            8779..9549
                     /locus_tag="PLU28_03515"
     CDS             8779..9549
                     /locus_tag="PLU28_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HOG34166.1"
                     /translation="MELTGSQIWLYGVVASTVAGLATSIGAIPVLFFKKLLTEKQLDM
                     LLGFAAGVMLAATMFSLIMPAIDSGGIWVAVIGIIAGALVIELFDSFSPHEHFIKGPE
                     GRNVSKEMVKKVWLFVTAIALHNFPEGMAVGVSFGSGMLKNGLIVAIAIGIQNIPEGT
                     ATAVSFLKAGYTKKQAFFWSFLTGVIEPIGGLLGASFVVLMKPALPFFLSFAGGAMLY
                     VISDEIIPETHSHGNERVATFSLIAGFIIMMILDTALG"
     gene            9553..9954
                     /locus_tag="PLU28_03520"
     CDS             9553..9954
                     /locus_tag="PLU28_03520"
                     /inference="COORDINATES: protein motif:HMM:NF012304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase-like protein"
                     /protein_id="HOG34167.1"
                     /translation="MLKIYTDGSYRDGKVGYAFIVVNEGKVIHTFSDAFKSFDPSRQV
                     AGEIKAVLDALEWLKQMGEKRVQIFYDYSGLEEWACMRWKAKTPNTKKYQQTVVNCGI
                     EIFWNKVQSHSGDEFNDMADRMARQAMEKDK"
     gene            10349..11488
                     /locus_tag="PLU28_03525"
     CDS             10349..11488
                     /locus_tag="PLU28_03525"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S cluster-binding domain-containing
                     protein"
                     /protein_id="HOG34168.1"
                     /translation="MNKVYWISLTGSCNFHCRYCFQDLNGSFRSSGDMNAYTAQRVAE
                     MINCTAGKVYRIHFFGGEPFLNFEVMKLITESVKDKVESFSVTTNLSILSNEITEFIK
                     NNDFSLLISLDGIKASHDASRVFADGTGTFSTVAGNLKSLSQKLKKDSISIAKTLTVK
                     NYMNLFDDYMFFKALGFKVNVNPDINLNTNELNHEVLKNELKKIMKDMISDNESIEFW
                     NLSESIRTVNNFPYSKKIRSSLSSCYDASNYVTTISPRGDLYPCHYLYEMEDDYSATH
                     NIFGFNDVRQIEETFIRSVTESHVFSPLKESEVDALTQYGIDVCGCNGCKYLDFCAEQ
                     KYYAAQGSCFYKRYRFRKSITEKDKSRKMCIDRIIYDAALEIKGE"
     gene            11493..12281
                     /locus_tag="PLU28_03530"
     CDS             11493..12281
                     /locus_tag="PLU28_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34169.1"
                     /translation="MFITLCSENPALDMSHMIYLKKNKIDFIYRNNCFFMEKSIFEKI
                     AEKEIVYKVPTEIQAIFPTGVPQEMKKVYASHEKKYLRNFLNYDSETYYYSMTQRIKK
                     ERFELAYLFEGYKQDYFKIMPEFILPDKSFSTDGTLECIYFLGSKIKSPFGLLAAIAG
                     GYSFHCTYFSILFSYQIFEKYFVYAFVFHYSYDGYADKHEEIKKFIDYVGQGSGFKLA
                     DKHPQFLEKEFENIYCSKFPGENYISDIKYLGKVRYSQDDWEGV"
     gene            12278..>13143
                     /locus_tag="PLU28_03535"
     CDS             12278..>13143
                     /locus_tag="PLU28_03535"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG34170.1"
                     /translation="MKKGIKIFFENNRWQALFLILVIAVNVAVSFLISQYTKSFIDTS
                     TNIKDGRAIAEIFAVIAIIYGILFIIDTLISFLAHRLLYSGIKNLRLKMFKKIMETRF
                     TYFIDNSPGKIWGDIINNTNKVSVFYQALILLPISLIQAIVYFIIIYRINTSSALVIL
                     MTLPFIILVTYLFGVKIRKHEAKTMNMYREMYGESVEALTGIKNIKLFNSHDFFYDRF
                     TGKHSLLNKEIVLTAVYTQSWQSFLKVVTSLLPLIIIVLGINFVKEFSLGSGDMIILY
                     TFIPLFVSATRDL"
BASE COUNT         4434 a         2044 c         2858 g         3807 t
ORIGIN      
        1 tgtagtaaga ttcttccccg agtatatgaa ggccaaagaa attttggaaa ctcctgatat
       61 gggtagcgga gcagttgcaa ggttttatag aggcgggaac tttccctctc acggaacaga
      121 caattggttt gatgatataa aaaagagcgg cggagtcttt gttgatcttt caatacatga
      181 ttttgactat cttagaacgg tgtttggcga accggaaagc ataatagcca gaagtgtgaa
      241 gctttcggaa accaaagaat gcaaaaataa tgaccacggg cttgcagtaa taaagtttaa
      301 aaacggtgta cttgctcatg tagaaggaag ctgggcggag cctgacaaca tgccggtggg
      361 atttcataca aaatttgaag taaatacaaa aaacggaatg atacactttg atgcgacaga
      421 atcaatgacc ttcaatctgc agcttaaaag gcagcaaaac aactatgtgg cggcaagacc
      481 ttcagagtac aatccttatg agcaggaact gagagtattt atagatgcag tacagaacgg
      541 cagtgaagta aaggtaagcg gtgaggaagc cctcaaatca cttagagtag ctcttgcagc
      601 caatatttcc gctaaagaaa aaagaccggt attgatcgaa gaggtgaaat agtatgataa
      661 aaataggaat ccttggaata gctcatactc acggatatag ttacgtaaga cagataaaca
      721 agatgcagga tgcacaggta acggggcttt atgaagagaa cactgaattt gcaaaaagag
      781 cagcagcaga gcttaaactt aaactgtaca gctcaccgca ggagcttctt gatagctgtg
      841 atgcagtaat tattgccagt gaaaactcca aacatatgaa gtatacagaa atggctgcac
      901 ttaagcataa acatgtgctc tgtgaaaagc ctatagcagt aaacataagt gatgcagagc
      961 atatgataaa agtatgtaaa gaaaacaatg ttgttcttca gatagctttt ccggtaagat
     1021 acgcacctag tgtccgcaaa gcgaaggaaa ctatcgacag cggtgttctg ggagatattc
     1081 ttgctgtttc ctctaccaac cacggaacga tgcccggaag ctggtttgtg gaaaaagaac
     1141 tcagtggtgg cggagcggta attgatcata cagttcatgt ggttgatata gtcagatggc
     1201 ttttgaatac tgaaatcaca aatgtttcag gtttttatga taaacttata tatgatgaac
     1261 tgaaagttga agactgcgga ctgctcatgc tggaactctc aaacggcgct ttcatgacac
     1321 ttgacacaag ctggtcacgt ccaaaggctc atccttactg gggagatgta accataagaa
     1381 tagtcggaac tcagggaacc cttttcatag atgcctttga tgaaaagata gagtactact
     1441 gcaacgataa aaagaacgga tggatgaact ttgacatcaa gtttgattat aatcttataa
     1501 gagagtttat agattgtata aaaacaggaa gaaaacctct tacatccgga gaggacggac
     1561 ttgcggctct ggaagttgta ctaaaggctt acgcagccaa ggcataaaat ttcccctcac
     1621 agtaatgtga ggggttttta aaaggagaac agatatggag tacagaatac tcgatgatta
     1681 taggctattc agcaagatgg ccggtttttc tttttctgtt acggcaaaga atcaaaagat
     1741 ctatgaagaa agacatcagg aaatatttca tgagaaaaga gagtttctgg gagcttttga
     1801 caataaggat cttgctgccg gacttattct ctatccgtat atgatgaact ttcgaaataa
     1861 tcttataaaa actttcggtg ttggggacgt ctgctcaaga gcggataaga gaggtaaagg
     1921 tgaagtccgt tatcttttaa acgaaagtgt caggcttatg cacagccgtt cagcgggact
     1981 ttcagttctg tatcctttta actatgattt ttacagaggt tctggatggg aagttttcac
     2041 caacagcgct atgtatcttt tgtcaccctc ccaacttaaa actctgcccg aaacagataa
     2101 tataaaggca gttgatatgg attttcctga taagcagggt attgaatact ataacaaaaa
     2161 agtctgtgga aaatataatc acgttattag gtctgaaatc gactggaagg accagttgag
     2221 aatgttttca gacaatgaaa tcaaaagagg ggtagtacag ttttataaga actcgcaggt
     2281 atgcggtatg atgacatacg tgtacagcta tattcccaag gaggacagca cggttttcac
     2341 cgtaagaacc tttttttatg atgattttga aacgaaaaac agcatgttcc ttttcataaa
     2401 aaagttatcg gcacagataa gttctgtaaa tcttttggca ccgaatgatt ttattctgtt
     2461 tgcacatctt tctaactcgc ctgtagcact aaagaaagga aatcattgta tgataagaat
     2521 aattgatatt aacgctctgg atggcatatt ttttgattca tgtgatgcag atatatcggt
     2581 tgaacttaca gacagtgtga taaaagaaaa taacgggtgt ttcagattta ttatagataa
     2641 taaaaaacta aggatttcta ggacagaaaa tgctgctata agcacagata tagggacatt
     2701 ttcgtctgta ctgagcggat atgtaggaat aaaagatatg atagactgct caaaaataaa
     2761 gattatagga aagtacgacg gcacagactt taaaaaggag tgcacattta ttaccgaatt
     2821 attttaagga ggaagtatgg cccaaataac agctgtgata gtaggggcag ggaacagagg
     2881 aagcgtttat gcccgttatg cgtcggagta tcctcagaag cttaaaatca atgctatggt
     2941 tgagaagaat gttggaaaac ttgagtatgc ttcgcaaaat tatgatatag ataaaaacta
     3001 ctgtttcagt gagtacacgc aggtacttga aatggggaaa gttgctgatg ctgcaataat
     3061 aaccacatta gacaatgcgc ataaagaaat tgccattgag tttataaaga gaggctacga
     3121 tattctgctt gaaaaacctt tggcggtaag tcttgaagat tctttggaaa tactcaaaaa
     3181 tgctaaaaaa tataatgtca ggcttatgac atgctatgtt ttaaggtaca gcaccttctt
     3241 cggaaagctg aaggagctga ttaacaaagg cattgtaggt ccagtacttg ccgttgatca
     3301 gagagagaat atcggttact ttcatatggc acacagctat gtaagaggta actggcacag
     3361 tacgaaggat agctcgccca taatacttgc aaaaagctgt cacgatctgg atattcttca
     3421 ctggatgctt gaaaaaaagt gtaccagagt attttcgatg ggcgaactga agtatttcag
     3481 aaaagaaaat atgccctgcg gtgctgcccc aagatgtctg gactgtcttg tggaagaacc
     3541 atgtccttat tctgcaaaga aaatatatct tcagacagat aataccggct ggcccgtttc
     3601 aaccataacc aatgattttt catactcggc aagaatggaa gttttgagaa acaccgatta
     3661 cggcaaatgt gtctatcagt gtgaaaatga tgttttggat tatcagacag tggatatgga
     3721 gtttgaagaa ggcatatcag ttagctttac cctcacagca tttactaatg acaaaaccag
     3781 aataatccat atatatggag gaatgggaga gataacggga gattttgaaa aaggagagat
     3841 aactgttcat cgttttggag aacaggcaca aatgataaag gttgttccgc catatcagac
     3901 cgcccatgca ggctctgact ttcagatgat ggacagattc gtgcaggtga tgactgatag
     3961 aacaggcaac tttatagagc ttgatcttaa cagtaccatg gagagccatt atatggcatt
     4021 cgcagcagaa aaatccagaa atgaaaaact ccctgtcgac atgataaagt ttaaaaaaga
     4081 acataatttt taacgatata aatgctctat actttatatt ttttctctag gaagcatata
     4141 attgagatat ttctgttcga agcttaatta taaaaattat tgtatttgtg ttataattct
     4201 cttgctttat acctatacat aggtttttaa aattcacagt taaacctggc aatttgatgc
     4261 gaaagtatat aaggagggat ttcatgaaaa agattttggc tgtcataact atagtactta
     4321 tgggaatatc tttgctggca tatccttgga taacaccttc atgcaatatt cttgaggaag
     4381 gctatgtggc tgttgatgct tttaatcctg cctggggtat acgttatggg cttttcgggt
     4441 ttatggaggc aggcgctggc caacctgaag gcggaacata tctaaagttt ggatttaaca
     4501 ggatactgga tatcccgttg agtttcagcg tagggtattc aaatgacttt ataagcaatg
     4561 catatttttt tacgggtaca gagcttaacc tggatatctt cagaataggt ttcgggggaa
     4621 tgctcggtca gaacaggcac tataatacgg tgacatcaac agatgagtat aaactttttc
     4681 caagtgttta cggaagaaca agcattgtgt tcgagaaaaa aacaaacata gaaaactctt
     4741 tctctatcga aggagctatg cgttcagata tgaatcttac ttttcaaaac tgcatggtgg
     4801 ccttttacgg caaagaaaaa atcaagaacc tgaactttct gtggtttaaa gatgttgata
     4861 tactcggagg aattgttttt acaacatctg attttgcaaa ggtagatctc ctaaaagatt
     4921 ttcagatagt actaggaatg tcaaccggtt ggaagttttt ttaaatcaaa ataaactatg
     4981 tgagtacttt gaactgaccc cgaaaagttg tacttaaaga agccaatttg gaggaggtca
     5041 gttttatatt agatttttat ataaagaggc tgaaatgaaa agaataagca tacttataat
     5101 ggtgtttttt acgatttcga cagctttttc aaatatatat gacgggtatc ttctgataaa
     5161 ggcacccgta ttttcgaaaa tttttatagg cgggctatac aaaggaactg ttcctgaaag
     5221 ctcggagatt gcttttgcac tttcacccgg aacatatgaa ctgactttaa aacttgaagg
     5281 atacctggat gcgagtaaaa aagccgtggt agaacctgta aaaagtactg ttgtttcatt
     5341 ttttccgcag aagattaatg aatttaaaag cacatctgaa actaaagaag cttcagggat
     5401 gagttacgga actgtggata ttataagtgt accctataaa aatcttcgta tagatataaa
     5461 cggttatgag ataggcagct cagattttta cagtctggcg cttgtgaagg gaagttatga
     5521 aataaagatt tctatgggag aacagtatct gaaaacttcg ataaatattg aaaaagataa
     5581 acatacggtt cttcaggcgg atttcttcaa aaaacagatt ttcaatcttt ataagataga
     5641 tttcgaactt ccccaaggtt caaaactttt tattgacgat aaagaacttt ctgatacaga
     5701 aaagtctgtg atgatacttg acagcagaca taaactgaaa gtggaaaatg agctttatga
     5761 accttttgag caagaagttg tttttgatgg gtcaggagta tataaaccgg ctctgatacc
     5821 taaggcatct tatgtggctg tgaactgtaa ttttaatgat gcacaggttt ttatcgacgg
     5881 aaactttata ggaaacgccc ctgttatctt taaaagttct atatccttgg aaaattcaac
     5941 ggttatggtt gtcaaaaaag ggtattctga gtaccaaaag acaggcataa agcttggaga
     6001 cagagatata gcctacattc aggcgcagct tgtaccgcag ggactgactg atgtggcagg
     6061 aggagcattt agaatggggg atatggacta cccggatatt cgaccggttc tggaagtttt
     6121 tttaggatat gacttttata taaatccata cgaaacgact tttcaagagt acgacgagtt
     6181 ttgcaaccag acttcaagag taaggccttc tgacaacggt tggggaagag ataaaaggcc
     6241 ggtcataaac gtgagctggt gggatgcggt tgcttactgt aactggttaa gtgaaaaaga
     6301 aggactgccc aaagcatatg atctgtgggg aaaccttctg gatgaaaaag gaaagattac
     6361 ttcagacatt aaaaaggttt ttggctacag actgcctaca caggcagagt gggagtatgc
     6421 cgcaaagggc gggcagatga gtatggcttt tatttacagt ggctcagaca atcctgaaag
     6481 ctgttcatgg ttcagaaaaa attcacttgg gatgactcat gaaacaggta agaaaagtcc
     6541 aaatgagctt ggactgtacg atatgtcggg aaatgtaaga gagtggtgta ccgatagata
     6601 taactcatac tctttgcttg aaaaagatga tccgtatgaa aacgaaggtc tgggaagagt
     6661 tgtaaaaggc ggaagcttcg aaatgaatga actgttcttg cgtataagca acaggtggct
     6721 caaagaccag gaatataaag atttttcgac agggttcaga gtagtaaaaa caattcagta
     6781 aaaacggctt tctcaaaagc cgtttttatt ttttaaggac tcctttttat cttttaaaat
     6841 catatcatcg ttttaaaaat gcactttatc agcaaatagt ttttttgacc tgtaaaaaaa
     6901 aatataatag atatgaaaat aggaaaaagg aggtaatatg atgaattctg taattaaggg
     6961 ctttttgatt gcagccggag ccataataac cgtttcgctg cttttgaatc tcacgtcatc
     7021 ttcaaagcta cagagcacaa gcagggaaat acgagagagt tttgaaacga taagcgtaga
     7081 aacaatttcg tcggatatag atctatcagg atcagacagc agcagacttc tttactcgca
     7141 cgataaaaat atttttgaaa accatattgg tttggaagga aaacggttat tcatcaaaga
     7201 aacaaaaaaa gatatattca ggggtataac ctcacagaaa ggcagttatg cggttttttt
     7261 ggcagtatat ccacaaagtg ctgttttaca aagcatgagc ggaaatgtat ccatctcaga
     7321 tataaaaacc acagaagtaa gcatcacaaa tacctcagga gatattcatg cagtcaatgt
     7381 aaactttgaa aactttttat cttcatcagt atccggtgga gtaaaagttc agaaagcatc
     7441 agttaaaaag ctgaaaataa aaacggtatc gggagaagta ggaatcgagg atgtagcttc
     7501 agacagtatt gaagttgaag ttgtttcagg cgatataagc atgcaaggtt taagacaagc
     7561 atgcgatata atagtcaaaa caacgagcgg cgatgttaga attaaaggaa ttgatttaca
     7621 gggctataac gtagagttca gctcaataag cggagaagta tttataaaag atgaagtttc
     7681 gtcaaaaggg aagtaccgct acggcgtcgg ggataaaaag ataaaagttg aaagtgtttc
     7741 cggagatctc ataattgaat gaaagcatgc catttaacgg catgtttttt tatttttagt
     7801 caaaaatata gtatcattgt atctggaagg ggggatttca atgctttcgg ataaaaagaa
     7861 tttttttact ccggatatac ttgaaagaat gcttcaggat aaaaaagctg gtatttatgc
     7921 tttggatacc aaaagagtga ttactttctg gaatgaaggg gcacaaaaaa taaccgggta
     7981 tgaatcctct gaaatacttg gtttgtcatg catggacaac atcctgaatc atattgattc
     8041 aaagggtgtc agattatgtg aaaaaaactg tccggcaagc agagccatgc aggatggtct
     8101 gcccaaaaga gctgaaattt ttctgcacca taaaaacggt tacagaatac ctgtagtagc
     8161 gacagtctat ccggtaaaaa attatgacgg tgtagtttcg ggagttatag aggtctttaa
     8221 cgatctttca gaaaaagaca gacaatcgca gagtcttgtg tatgacgagc ttacaggaat
     8281 cttatgtaaa caagaagttg agcagaaaat aaaacaggct gaagaagact ttaaaaaagt
     8341 ttcagtacct tttggtctta tatgttttgg tattgataat ctttctcaga tagctgagaa
     8401 gtataaagaa cgagtagcgg aaaagatttt gattatggct gcaaaaaacc tgtctgctaa
     8461 tctcagaata acagacgaaa caggcagatg gggtaatgac agttttatgc ttattgtaaa
     8521 gtttgttgac agggagcagc tacacgatat agctcagaac atgctcataa ctatgaacaa
     8581 ctgttttctt aaagaagaga atgaaattat tatggcaagt gtttcagctg gcggaagtgt
     8641 ttttaaagat aatgacagca tttataccat aacagaaaga gctgtacaca accttgaaaa
     8701 agccagattg actgataaag gaagtctttt tatagattaa attttttata gatgattgaa
     8761 tacacaagga gggaaagtat ggagttaaca ggttcccaga tatggctgta tggtgtggtt
     8821 gcaagtactg ttgcaggact tgctacatct ataggagcta tacctgttct tttttttaaa
     8881 aaactgctta cagaaaaaca gcttgatatg cttttggggt ttgcggccgg ggttatgctt
     8941 gcagctacga tgttttctct tataatgccg gcaatagaca gcggcggtat atgggtggct
     9001 gttattggaa taattgcagg tgcactggta atagagcttt ttgacagctt ttctcctcat
     9061 gaacatttta ttaaaggtcc tgagggaaga aacgtatcaa aagaaatggt aaaaaaagtt
     9121 tggctttttg ttaccgcaat tgcgctgcac aactttcccg aaggaatggc tgtaggagta
     9181 agcttcggaa gcggtatgct gaaaaatggt cttatagttg ctatagctat tggaattcaa
     9241 aatattccgg aaggtacagc gacagcagtt tcctttttaa aagccggata cacaaaaaaa
     9301 caggcctttt tctggtcttt tctgacggga gtaatagaac ctattggagg acttttgggt
     9361 gcttcctttg tagttcttat gaaacctgcc ctgccgtttt ttctttcttt tgcaggggga
     9421 gcaatgctct atgtaataag cgatgaaata attcccgaga cccattctca cggaaacgag
     9481 cgggtggcaa cattctcact tatagcaggt ttcattatta tgatgattct tgatacggca
     9541 ttagggtgat ggatgttaaa aatttataca gacggatctt acagagacgg aaaggttggt
     9601 tatgctttta tagttgtaaa tgaaggcaaa gttattcata ccttttcgga tgcttttaaa
     9661 agctttgacc cttcacgcca ggttgcggga gagataaagg ctgttcttga tgcattagaa
     9721 tggctgaaac aaatgggaga gaagagagtt cagatctttt atgattacag cggtctggaa
     9781 gaatgggcat gcatgcggtg gaaagcgaaa accccaaata ccaaaaaata tcagcagact
     9841 gttgtcaact gcggcataga gatattctgg aataaggtac aaagtcatag cggcgatgag
     9901 tttaacgata tggcagaccg catggcaaga caagcaatgg aaaaagataa gtaaaaataa
     9961 aatttaagag attcaatccc ggattaagta agatctgatc cgggattttt ttatgttatc
    10021 tattaagcat ataaaaaata atataaataa ataataaaca taaatacaat taaaatgata
    10081 caaaaaaata ttttaagata ctaattgata tagatcaata ttataggtat ctatagtaat
    10141 gtatgaaaaa tctatttata aaaaaaaaca gatcaaatta ttaaaaaaag tttaaaagac
    10201 ttgatacata agtattttaa acagttgatg aaaaaattta atatataaaa aatatgatat
    10261 aatattttta atcaagacaa gtataaaaag tttaaaagta acataaatat attattctaa
    10321 aaacaaaaac aggcattaag gggagagcat gaataaagtt tactggattt ctctgactgg
    10381 aagctgtaac tttcactgta ggtactgttt ccaggattta aatggttctt tcagaagcag
    10441 tggcgatatg aacgcttata cggctcaaag ggtagcagag atgattaact gtactgccgg
    10501 caaagtatac aggattcatt tctttggagg agagcctttt ttaaactttg aagtgatgaa
    10561 gcttataact gaaagtgtga aggacaaagt tgaatctttt tctgtaacca ctaatttaag
    10621 catactcagt aatgaaatta ctgagtttat aaaaaataac gacttcagtc ttctgatcag
    10681 tcttgacggt ataaaagcct ctcacgatgc ttcaagagta tttgcagacg gtacggggac
    10741 attttcaacg gttgccggta atcttaaaag cctttctcaa aagcttaaaa aagacagtat
    10801 ttccatagct aaaaccctta cagtaaaaaa ttatatgaat cttttcgatg attatatgtt
    10861 ttttaaagct cttggtttta aggtaaatgt aaatcctgat attaatctta atacaaatga
    10921 gctgaaccat gaagttttaa aaaacgagct gaagaagatc atgaaggata tgatttctga
    10981 caatgaaagc atagaatttt ggaatctgag tgaaagtata cggactgtaa ataactttcc
    11041 atacagtaaa aagatccgta gctcactctc aagctgttac gatgcttcca attatgttac
    11101 gactatttct ccccgcgggg atctttatcc gtgtcactat ctttatgaga tggaagatga
    11161 ttatagcgct acgcataata tctttgggtt taacgatgtg agacagatag aagagacttt
    11221 catccggagt gtgacagaga gtcatgtttt ttcaccgctt aaggagagtg aagtagatgc
    11281 actaactcaa tatgggatag atgtctgcgg ctgtaatggc tgcaaatacc ttgacttttg
    11341 cgccgaacag aagtactatg cggcgcaggg aagctgtttt tacaaaagat ataggttcag
    11401 aaagagtatc actgaaaaag acaagagcag aaagatgtgt atagatagaa taatctatga
    11461 tgctgctttg gaaattaaag gggagtgact gtatgttcat cacactttgt tctgaaaatc
    11521 ccgcactgga tatgagtcac atgatatatc tgaaaaaaaa taaaattgat tttatatata
    11581 gaaacaactg ttttttcatg gaaaagagta tctttgaaaa gatagctgag aaagaaattg
    11641 tttataaagt gccgacggag atacaggcaa tctttcccac gggagtgccg caggagatga
    11701 aaaaggttta tgcaagtcat gaaaaaaagt atctgagaaa ttttttgaac tatgattctg
    11761 aaacctacta ctactctatg acccaaagaa taaaaaagga aagattcgaa ctggcatatt
    11821 tatttgaagg atataagcag gactatttta agatcatgcc tgaatttata cttccggaca
    11881 agagcttttc cacagacggt actcttgaat gtatatactt tttgggatcc aagataaaat
    11941 ctccctttgg acttttagca gcaattgccg ggggatatag ctttcactgc acttattttt
    12001 caatactttt cagctatcaa atttttgaaa agtattttgt atatgctttt gtttttcatt
    12061 acagctatga cggatatgcg gataaacatg aggagataaa gaagtttata gattatgtcg
    12121 ggcagggcag tgggtttaag cttgcagaca aacatccgca gtttttggaa aaagagtttg
    12181 aaaacatcta ctgttcaaaa tttccggggg aaaattatat ttcagatatt aaatatttag
    12241 gaaaagtcag atattcgcag gatgactggg agggagtatg aaaaagggga taaaaatttt
    12301 ttttgaaaac aacagatggc aggccttgtt ccttattttg gtgatcgccg ttaatgtggc
    12361 ggtctctttt ctgatttctc agtacactaa aagttttatt gatacaagta ccaatatcaa
    12421 agacggtaga gcgatcgcgg aaatatttgc tgttatagcc attatatatg gaatcctttt
    12481 tataatagac actttgatct cttttttggc tcacaggctt ttatatagcg gtataaaaaa
    12541 tctaaggctt aagatgttca aaaagatcat ggagacaagg ttcacctact ttatagataa
    12601 ctctcccgga aagatatggg gggatattat aaataacacc aacaaggtat cggtattcta
    12661 tcaggcgctt attcttcttc ctataagtct gattcaggcc attgtatact ttatcataat
    12721 atacaggata aacacgtctt cggccttggt aatcctcatg acactgcctt ttataatact
    12781 ggttacgtat cttttcggcg ttaaaatacg taaacatgag gctaaaacca tgaatatgta
    12841 cagggagatg tatggtgaaa gtgtggaagc acttacaggg ataaaaaata taaagctttt
    12901 taactctcat gattttttct atgaccggtt taccggaaaa cactccctgc ttaacaagga
    12961 aatagttctt actgcggttt atactcagag ctggcagagc tttttaaagg tagttaccag
    13021 tctgcttccg cttatcataa tagttttagg gataaacttt gtaaaagagt tcagtctagg
    13081 cagcggtgac atgataatac tgtatacatt cataccgctt tttgtaagtg ctaccagaga
    13141 tct
//
LOCUS       DAOSHR010000040        12594 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_4193_length_12594_cov_5.902385, whole genome shotgun sequence.
ACCESSION   DAOSHR010000040 DAOSHR010000000
VERSION     DAOSHR010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 12594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12594
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4193_length_12594_cov_5.902385"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="PLU28_03540"
     CDS             <1..246
                     /locus_tag="PLU28_03540"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOG34171.1"
                     /translation="LNKIKNGLSDQNRENKIDKELFTEREIEIIRLISQGLSNKEISE
                     HLFISEGTVKNYITTILSKTGLQHRTQIAIYYLKGNF"
     gene            331..1272
                     /locus_tag="PLU28_03545"
     CDS             331..1272
                     /locus_tag="PLU28_03545"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG34172.1"
                     /translation="MRILQLKNITKRFEDFVTIDNLSLDIMKGEIYGLVGPYGAGKTT
                     LLGIMAGLIKPDRGEVFFREQNAALVNKEMSYGIGFVPQEIAVYDEFSVYENIKFFAS
                     LYGLKGKELNNAVNQTISLLELEDYSKKTPAMLTVGFKRLLNIACSICHKPTLLILDE
                     PTVGIDTQTRNFILQIIRALNDAGTTIVYTSHYIDEIESLCSKIGIIDHGKIIAQGSS
                     EELKSIITDTTTIIIKSSSIDGLDERRLKKINGVIDVQVDMPGCSIKIISSKDVNNLD
                     KIILFFTNNNISIKNIESTVPDLEMVFLTLTGRRLTG"
     gene            1277..2383
                     /locus_tag="PLU28_03550"
     CDS             1277..2383
                     /locus_tag="PLU28_03550"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG34173.1"
                     /translation="MIKLILSELKKISRDVRPMVMMLVIPILLTITLGTSLKNLFETS
                     IETKENLILYHIQDQGAYHSQFQNLVSSLKDEGFIFISTNTFEEGLNAVKNGKYNCFV
                     YISSNYSAVEIYENNKEPLKVSFVEEMLRKFLYPLYIGSSWISKGEKSDAPSDLVELV
                     KMPLPKKPSSIDYYSVTMLTMIILYSSATMIFSYSKEKNYKTLYRIFFSPVSRKKLFF
                     AKYFSALIITMMQIVLIISFDMYVMKAFWSEHLSAVFLVLGTYTLMSLSMGGLIFMIF
                     RKESSMWITINIICQTSALLSGSYKQINYDYIPFLGLLSSWLPQSAVNNALFEIIYKD
                     SYTMLPRAMAINLILAGVMYWLSGKLFSSKRVIL"
     gene            2380..3528
                     /locus_tag="PLU28_03555"
     CDS             2380..3528
                     /locus_tag="PLU28_03555"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG34174.1"
                     /translation="MKNFFILIKKELIMIFKKKYNILILYVIPFFAILLSYLISANSS
                     NYVTNIGVYDEDKTIISSGFIDFISETRNVRVIPVSSYDMVDLLNKEKIRCVVYFPKG
                     FQSQLLTNKDTEIQIITQNSGNTENLLKSYANFYFKKMLQMINESQKTGYVQNKDKTI
                     DIYNEYQKENVNLRIIEIQDKKRTKNSLNVSMGILIVFIALGSMSVSYNLISEKQNSI
                     FQRILTSPVSLRNYFLANISSNFLVLISQTLVVMFAVTVVLRVNLINDIFGFWIMLVA
                     YGIFSIAFSFFVVSVSPSKAYANSIFNIIYIPTCMIGGCFWEVSIMPGSLQGAAFFMP
                     QQIFLKGLYFLVDYPDSSGFYQYLSILLLYSLVFFLVAFYSFQNKSRV"
     gene            3583..4506
                     /locus_tag="PLU28_03560"
     CDS             3583..4506
                     /locus_tag="PLU28_03560"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOG34175.1"
                     /translation="MKEYDLFPEFRIDIQEEKLLESKDLRGSYAIILLSNENRNTFLI
                     NLQNISKDMKEYTCGDNPTVKLAGLWGTCSGEIGGMTVPEFIKSNAEIKIVITDRLLR
                     IRYIFRQYADKQEVTGKLSEIIRNDRLPSNEIMSRKALRGLSSRPIEHMDIETIIMTA
                     HTAPSCMNKQPWRYDAVVSEDMLEKVRETLSEGNYWMKKAPVVIAVHAGLDNDCRLND
                     SRDYYLFDTGLSVGLLLAQASKMGIIAHPVAGFDPVKARKILEIPDGETLIVLIALAY
                     PGDYSDLSAKHIKAEFSVRERQPLEKILRWV"
     gene            complement(4587..5816)
                     /locus_tag="PLU28_03565"
     CDS             complement(4587..5816)
                     /locus_tag="PLU28_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HOG34176.1"
                     /translation="MELKKLIAQKIKELQPDMIEASKKFVSINSVNPRTGGPGEKEVS
                     EYLQAVLQSWKFDEIKRYDAPDSAVLYGYRPNIIAIYKGKNPSKTLWFMTHMDKVPAG
                     DISLWNTDPYTAVEKDGKIYGRGSEDNGSSMISSIFAVKALMDMGIRPENNIGIALVS
                     DEETGSEYGIQYLIDQKVFGKNDWFVIPDSGEPDGAFIELAEKSMMRFKITTQGKQAH
                     ASMPNLAKNAVRAGMNFAVAVDKFLNETYNVTDPLFDPPYSTFEPTKKDNNVENINTI
                     PGTDVQHFDCRVLPMYDVNDIMAKVKDMAREYSRKYDVTITIDDSDKRNAPAPTPEDH
                     DLVKLLKASIKEMTGIIPRTGGIGGGTCAAHLRAYGIPCVVWATLDEMAHQPNEYIKI
                     EYLLKDASVYAYMMSKF"
     gene            complement(5962..6318)
                     /locus_tag="PLU28_03570"
     CDS             complement(5962..6318)
                     /locus_tag="PLU28_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34177.1"
                     /translation="MLTYLNSDPKIIRNKSEAQILKEKIRAEVEKLKKEEKKKRVEKI
                     LNSEEINYLNKKEMVVALLYKKFNKDFTSHITQYEEIVDHKLMNKLFDHIFDRITDIS
                     QEQVEHIFDCDLLMKK"
     gene            6498..7778
                     /locus_tag="PLU28_03575"
     CDS             6498..7778
                     /locus_tag="PLU28_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HOG34178.1"
                     /translation="MSMKLIMLAIFLITYYFIIFGKVSKSIIAFSMGVLVLMLKISSE
                     LRIESIGEYVDFDTLALLMGMMIIVGVLKTTGVFEAVSAYIVKISKGNFIQIFVMILI
                     ATALLSGILDNVTTVLLFSPIVFLICQEAEIDPKVFAFPMIFASNIGGTLTLIGDPPN
                     ILIGNASRTGFMDFLKIMAVPTILSLTISIFIFLAQYKEIKNISPEKLAKLSQLDPKK
                     AITDYKLLIKGSIIFALVILGFVLKDKLQYESSVIALAGAALMLIFSKTTFEHIAKDI
                     EWDTIFFFIGLFIIVKGLEEVNIIADVAAALSTFASHPFMMIMTVLALSAVLSSFIGA
                     VPVVTIFIPVLEIIIPSVPKGYLLWWALALGANFASNTTIVASACNMVVVGLLESNFK
                     EKVSFFEFMKKGIPITMMGLAISALYLTGLFYLF"
     gene            complement(7766..8197)
                     /locus_tag="PLU28_03580"
     CDS             complement(7766..8197)
                     /locus_tag="PLU28_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34179.1"
                     /translation="MSNNFRANEYRADLIWEKGLTEKKLTLEFTFIDNGAHEKKIAFT
                     FSDKESSISLIKESMVMITGMENVDIFMWKVNAKYKITKDTVDCIYRICDYIGSKLKT
                     PSFISRDEIRRSIKKAEKHPSKRIEVLKTLTVMNQTVPQNR"
     gene            complement(8207..9928)
                     /gene="ade"
                     /locus_tag="PLU28_03585"
     CDS             complement(8207..9928)
                     /gene="ade"
                     /locus_tag="PLU28_03585"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HOG34180.1"
                     /translation="MKLRDMIPVALGEKNADCILKNAKILNVFTGETEVSDIALFDKR
                     IAGVGSYDNSDNITDLDGLYVIPGFIDAHVHIESTMLSPLNFAKLVILNGTTTVIADP
                     HEIVNVMGVDGLEYINNSTEGIALNVYIAIPSCVPATSLETSGAKLGPDDMIGFIDSH
                     LKRTVGLGEVMNYPGVLSLEPELITKIEILRHRYKKIDGHAPGLSGKKLNGYINAFIR
                     SDHECTTKEEAIEKLSKGMHIFIREGSAAKNFDALIPAVNLYNHQYFSFCTDDRDPLE
                     ITEQGHINNLVKRAVAKGLDPVVAIRMASINTAKFYNLRSCGAVAPGYKADLLVVDNL
                     HDLNIKMVFHNSKLMVKDGVLTGDMNGIYSKTELTGYSFNPPEICEQDIKIKDTGRKI
                     KVIGIEKASLYTTPLITEPTVKNGYILADPARDISKVVILERHHASGYSVGFIHGLGI
                     KEGAVATSVGHDSHNLCVCGMNDADIVLAAQTVKKMNGGIAMVKNGSVIFEFPLPIAG
                     LMSDRTIKEVTAKLLERKNRLELLGCSNDIFMTLSFIQLAVIPQIKITDRGIIDVAAQ
                     KIVSLYE"
     gene            complement(9879..10415)
                     /locus_tag="PLU28_03590"
     CDS             complement(9879..10415)
                     /locus_tag="PLU28_03590"
                     /inference="COORDINATES: protein motif:HMM:NF015239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017111 - ribonucleoside triphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphatase"
                     /protein_id="HOG34181.1"
                     /translation="MVFIVTGEINSGKSTFMENLYNSNKEGGGIITRKKFCYGTFLGY
                     DLYRLDNQTKVEFALYPKFIDDKWEEVCRFGPFSFSKKALIFFDSCIHDLINRKVQPV
                     FIDEAGPAELEGKLFFMGIKKVLHSGLTLYITVRSSCLEKMISVLSIKDFQILKIEKG
                     GFYEIKGYDPRSIRRKER"
     gene            complement(10403..11218)
                     /locus_tag="PLU28_03595"
     CDS             complement(10403..11218)
                     /locus_tag="PLU28_03595"
                     /inference="COORDINATES: protein motif:HMM:NF024869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC/CoxI family protein"
                     /protein_id="HOG34182.1"
                     /translation="MTDIYEKIISSIENGKSGVLITVTEKDGFGPGKIGSKMFVASSM
                     QRTGSIGGGPLEMLVLKDCKEVLENKQCAAKTYTLDSEKERLDSQTVDMLCGGTVKLF
                     FEYIGPKAEIFLFGIGNVGREILVSLSRLDYSVCVFEERSDVQVPEVKLSCKIIKDYI
                     AAIKEYKFPKKGIYIICTYSHEKDYEVLRELLMKKTDAFYIGMLASEKKAGMIVSKIK
                     KENPDVDLSVLYSPIGLDIGGQTPSEIAVSVTAQIQAAMYGKQAPGLSRKWSS"
     gene            complement(11181..11825)
                     /locus_tag="PLU28_03600"
     CDS             complement(11181..11825)
                     /locus_tag="PLU28_03600"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOG34183.1"
                     /translation="MASSLEGLVLAAGLSTRSGSYKMELMFGKSTLLQQCIKSIYAEC
                     SKVIVVCGYNGGKVQELVKDFKKVELVRNDSYEKGMFSSLKTGLKHVSSDKLLITPGD
                     YPLFRQTTVHKLAVTKGSIVIPVYKSKKGHPIIISKALYPEILMSPDSRNLRDILKNH
                     TFVPVQTDDVGVVSDVDTIDDYYICLDIFRKRSDEEKAKEEQHDRHIRKDNQFH"
     gene            complement(11829..12470)
                     /locus_tag="PLU28_03605"
     CDS             complement(11829..12470)
                     /locus_tag="PLU28_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34184.1"
                     /translation="MKKNLSAGIFYGSVWGMLEATLGYLLHFVSDFIPGISSFIMFPI
                     GFSIMFRAYRKSSELQTVLICGITAAAIKLVNFFLPVSNILKIVNPSISIVMESIAVL
                     LVIFFAVQKGRKLNPLDIAAMTFGWRTVYLIYLSILRTFPLKLELLSKGTSAVYKFWI
                     TDSIANIIIISTALMIFYVLRKENSSKKAEPNFLYASTAFFIAISTQLIFSVI"
BASE COUNT         3736 a         2467 c         2308 g         4083 t
ORIGIN      
        1 cttaataaga taaaaaatgg tctttcggat cagaacagag aaaataaaat tgacaaagag
       61 ctttttactg aaagagaaat tgaaattata cggcttatct ctcagggact ttcaaataaa
      121 gagataagcg agcatctttt tatatccgaa ggtactgtaa agaattacat tacaactata
      181 ctgagcaaaa ccggacttca gcatagaacg cagatagcca tatactatct gaagggaaat
      241 ttttgatgac ttaagtgata gaaattggtg acctttatca ctactaaagg ttgccgtttt
      301 tttataccat gaaatcaggc tggtgataaa gtgcgcattc ttcaacttaa aaatattacc
      361 aaaagatttg aagattttgt taccatagat aatttaagtc tggatataat gaaaggtgag
      421 atatacggac ttgtaggccc atatggtgcc ggaaaaacga cacttctggg tataatggcc
      481 ggactaataa agcccgatag gggagaggtg tttttcaggg aacagaacgc ggctcttgtt
      541 aataaagaga tgagctacgg catcgggttt gttcctcagg agatagcggt atatgatgag
      601 ttcagcgtat atgaaaatat aaagttcttc gccagccttt acggattaaa agggaaagaa
      661 cttaacaatg cagtaaatca gactatatct cttttggagc ttgaagatta ctcgaaaaaa
      721 actcccgcga tgcttaccgt tggcttcaaa agacttctga atattgcctg ttcaatatgt
      781 cataagccta ctcttcttat acttgatgag cctaccgtag gtattgacac acagaccagg
      841 aacttcattc ttcagatcat acgtgcactt aatgatgcag gtacaacaat agtatatacc
      901 agtcattata tcgacgagat agaatcgtta tgcagcaaga ttggaattat tgaccacggt
      961 aaaattattg ctcagggttc ttccgaagaa ctgaagtcaa taataacaga caccacaacc
     1021 ataattataa aaagctcctc tattgatgga cttgacgaaa gaagattaaa gaaaataaac
     1081 ggggtaatag atgttcaggt cgatatgcca ggctgttcga taaagataat atcgtccaaa
     1141 gatgtgaata atctagataa aataatactt ttttttacaa acaacaatat ttccataaaa
     1201 aatatcgaaa gcaccgttcc ggatcttgaa atggtattcc ttacacttac cggacggagg
     1261 cttacgggat gaaaatatga taaaactaat attgagtgag cttaagaaga tttccaggga
     1321 tgtcagaccg atggtcatga tgcttgttat ccctattctt cttacaatta ctcttggaac
     1381 ctcactgaaa aatctttttg agacttctat agaaactaag gaaaacctta ttctatatca
     1441 tattcaagat caaggggctt accacagcca gtttcagaat ctcgtatctt cactgaagga
     1501 cgaaggcttc atctttataa gcaccaatac ctttgaagaa ggtctgaatg ccgtaaaaaa
     1561 cggcaagtat aattgttttg tttatataag cagcaactat tcagcagtgg agatatacga
     1621 aaacaacaag gaacccttaa aagtatcttt tgtagaggag atgcttagaa aatttcttta
     1681 tccactttac ataggctcat catggatttc aaaaggtgag aaaagcgatg cgccttcaga
     1741 tcttgtggaa ctggtaaaga tgcctttgcc gaaaaagcca tcttcaatag actattattc
     1801 agtaacaatg cttacgatga ttattctgta ttcttcagct actatgattt tttcctactc
     1861 aaaagaaaaa aactacaaaa ctctttacag aatttttttc tctccggtaa gcaggaaaaa
     1921 actttttttt gccaaatatt tttcagctct tatcataaca atgatgcaga tagtacttat
     1981 catatctttt gatatgtatg ttatgaaggc tttctggtcg gagcatctca gtgcggtttt
     2041 tttagttctt gggacttata cgcttatgtc actttccatg ggtggtctca tatttatgat
     2101 atttagaaaa gaaagctcca tgtggataac cataaatata atatgccaga catcagcttt
     2161 gctgagcggt tcgtacaagc agataaacta tgattacata ccgtttttag gtctgctatc
     2221 ttcatggctt cctcaaagtg ctgtaaataa tgctttattt gaaataatat acaaagactc
     2281 ctatacaatg ctgccacggg caatggctat caatctcatc ctggcaggag taatgtattg
     2341 gctttctgga aagcttttct catccaagag ggtgatactg tgaaaaattt ttttatttta
     2401 ataaaaaaag aacttataat gatcttcaag aaaaaatata atatacttat cctttacgtt
     2461 attccgtttt ttgccatact gttgtcatat cttatttcag caaactcttc aaattatgta
     2521 accaatatag gagtttatga tgaagataag acgattattt ccagtgggtt catagatttt
     2581 atttcagaaa cacgtaatgt aagggttatc cctgtatcgt catatgacat ggtggatctt
     2641 ttgaacaaag aaaaaataag atgtgtagta tactttccca agggattcca aagtcagctg
     2701 cttacaaaca aggatacaga aatacagata attacccaga acagcggcaa cactgaaaat
     2761 ctcttaaaaa gttatgccaa cttttatttt aagaagatgc tccagatgat aaatgagagt
     2821 cagaaaacag gctatgtaca gaataaagat aaaaccatag acatatacaa tgagtaccag
     2881 aaagaaaatg tgaatctcag gataatagag atacaggaca aaaaaagaac taagaactca
     2941 ctcaatgtat caatgggaat acttattgta tttattgcac ttggcagtat gagcgtctct
     3001 tataatctca tttctgaaaa acaaaatagc atatttcaaa gaattcttac atctccagta
     3061 agtcttcgaa attatttttt agcaaatata agttcaaact ttcttgttct catttctcag
     3121 actttagttg ttatgtttgc tgtaaccgtt gttctgcgtg taaaccttat aaacgatata
     3181 tttggttttt ggattatgct tgtggcatac gggatttttt ccatagcctt tagctttttt
     3241 gtggtctctg tttcacccag caaggcatat gcgaattcga tattcaatat catatatata
     3301 ccgacatgta tgataggagg ctgtttctgg gaagtttcga taatgccagg ctcattgcag
     3361 ggagcagctt tttttatgcc gcaacagatt tttctgaagg gactttattt tcttgtggac
     3421 tatcctgaca gcagcggttt ttatcaatac ttatccatac ttttactgta ctctttggtc
     3481 ttttttcttg ttgcatttta ttcatttcag aataagagca gagtataaac tgaaaatcat
     3541 tccctggctt atggtagaat ctagttaagg gaggtggaaa agatgaaaga atatgatttg
     3601 tttccagagt tccgcataga catacaggaa gaaaaactcc tggaaagcaa agatctgaga
     3661 ggcagttacg ccataatcct gctgagcaat gaaaacagga atacttttct catcaatctc
     3721 caaaacatct ctaaagatat gaaagaatac acttgcggtg ataacccgac tgtaaaactt
     3781 gccggtttat ggggtacatg cagcggagag atcggcggaa tgacagttcc tgaatttata
     3841 aaaagtaacg ctgaaataaa gatagttatt acagaccggt tgctccgcat acggtatatt
     3901 tttagacagt atgcagataa acaggaagtg accggaaaac tttcagaaat aataaggaac
     3961 gaccggttgc ccagtaatga aataatgagc cgtaaagcct taagaggact tagttcaagg
     4021 cctattgagc atatggatat agaaactatc ataatgacag cccatactgc tccttcctgt
     4081 atgaacaaac agccgtggcg atacgatgcg gtggtttcgg aagatatgct tgaaaaagtc
     4141 cgtgaaacac tttcggaagg caattactgg atgaaaaaag ccccggttgt catagcagtt
     4201 catgcaggtc ttgacaacga ctgtagactg aatgattcaa gagattacta tctttttgat
     4261 accggacttt ctgtcggact gcttcttgcc caggccagca aaatgggcat aatagcacat
     4321 ccggttgcag ggtttgaccc tgtgaaagcc agaaaaatac ttgagatacc agacggtgaa
     4381 actcttatcg tgctcatagc tcttgcatat cccggcgatt actctgatct ttcagccaaa
     4441 catatcaaag cggagttctc tgttcgtgaa agacagcctt tggaaaaaat attgcgttgg
     4501 gtataaaata attcattaaa cttttaatcc taagaaaaat ttcatcgatt aaccaaaaaa
     4561 tgcagcctta cggctgcatt tgtctatcag aacttactca tcatataagc gtatacagaa
     4621 gcatccttca gcaggtactc tatttttatg tactcgttgg gctgatgtgc catttcatcc
     4681 agagttgccc atactacaca cgggataccg taagccctta agtgtgctgc gcaggttccc
     4741 ccgcctattc ctccggttct gggtattatt cccgtcatct cttttatgga agctttaagc
     4801 agtttgacca gatcgtggtc ttccggcgtg ggagcaggtg catttcgctt gtcgctgtca
     4861 tctatggtta tagtaacatc gtactttctt gagtactcac gtgccatgtc cttgactttt
     4921 gccattatgt catttacatc atacatagga agaaccctac agtcaaaatg ctgcacatct
     4981 gttcctggta tggtatttat attttcaacg ttgttgtctt ttttagtagg ctcgaaagta
     5041 gagtatggag gatcaaataa aggatctgtg acattatagg tttcgtttaa aaacttatcc
     5101 acggccacag caaagttcat acctgccctg acagcattct tggccagatt tggcatactt
     5161 gcatgagcct gcttgccctg ggtggttatt ttaaagcgca tcatagactt ttcggccagc
     5221 tcaataaaag caccgtccgg ttctccactg tcgggaataa caaaccagtc attttttcca
     5281 aatactttct gatctataag atactgtatt ccgtattcag aacctgtttc ctcatctgat
     5341 accagggcta ttccgatatt gttttccggt cttatcccca tatccataag agcctttacg
     5401 gcaaagatgc ttgaaatcat cgaactgccg ttatcttctg agcctcttcc gtatattttc
     5461 ccgtcttttt ctacagcagt atatgggtct gtattccaaa gacttatatc tcctgcggga
     5521 accttatcca tatgagtcat gaaccacaat gtctttgaag gatttttacc tttataaata
     5581 gctattatgt tgggtctgta tccgtatagc acggcgctgt ccggtgcgtc ataccttttt
     5641 atctcgtcaa atttccagct ttgaagaaca gcttgaagat actctgatac ttctttttca
     5701 ccggggcctc cggtgcgcgg atttacagaa tttattgaaa caaatttttt tgaagcttct
     5761 atcatatccg gctgaagctc tttaatcttt tgagcgatga gtttctttaa ctccattgag
     5821 acacctcccg ataggctttt ggttttatat ttggcaccat aaaactatta tacaatacaa
     5881 atactaattt tatatgtaaa aggtatttat tttgaaatca tacgaaagaa aaaaacctgc
     5941 ctaagcaggt ttttgtatag cctatttttt cataaggaga tcacagtcaa aaatgtgttc
     6001 aacctgttct tgggagatat ctgtaattct gtcaaatata tgatcaaaaa gcttgttcat
     6061 aagcttgtga tccacaatct cttcatactg ggttatatgt gatgtgaagt ctttgttgaa
     6121 ttttttataa agcaaggcaa ctaccatctc ttttttatta agatagttta tctcttcaga
     6181 atttagaatt ttttcgactc ttttcttttt ttcttccttt ttaagttttt ccacctcagc
     6241 ccttatcttt tccttaagaa tctgggcctc tgatttattt ctgattattt taggatcaga
     6301 gtttaaatat gtaagcataa cccacctccc ggtcacatcc atgtgacctg tctattcaat
     6361 aataatatcg tctgaatgga ctaaaagctg aatattaaat aatataggtt tcctcttttt
     6421 aataaaatca ctatacaacg tttataattg ggcggtataa tatccctgtt ttaaaataaa
     6481 gatacggagt gattcatatg agtatgaaac ttataatgct ggcgatattc cttattacct
     6541 actactttat tatttttgga aaagtaagca aatcgataat tgccttcagc atgggagtgc
     6601 ttgtgcttat gctcaagatt tcctctgagc tgaggataga aagtatagga gagtatgttg
     6661 attttgatac gctagctctt cttatgggaa tgatgattat agttggtgtc cttaagacaa
     6721 cgggagtgtt tgaggctgta tcggcttata tagtgaagat ttccaagggg aacttcatac
     6781 agatttttgt tatgattctc atagccactg ctcttttgtc gggtattctt gacaatgtga
     6841 ccaccgttct gctgttttct ccgatagttt tccttatatg ccaggaagcc gaaattgatc
     6901 caaaggtatt tgcatttccc atgatatttg cgtccaacat aggaggtaca ttaacgctta
     6961 tcggtgatcc gccgaacata ctgataggca atgcatcacg tacaggattt atggattttt
     7021 taaaaataat ggctgttcct actattcttt cactgacaat ctccattttc atatttttag
     7081 cccaatacaa agaaataaag aatatatctc cggaaaaact ggcaaagctt tcccagctgg
     7141 atcccaaaaa agcaataacc gattataagc tccttattaa aggaagtatt atttttgctc
     7201 ttgtaatttt agggtttgtt ctcaaggata agctccagta cgagtccagt gtcatagctc
     7261 ttgcaggagc agcacttatg ctcatttttt ccaaaacaac atttgagcat atagccaagg
     7321 atatagaatg ggataccatc ttctttttca taggtctgtt tataatcgtc aagggtttgg
     7381 aggaagtcaa tataattgca gatgttgcag ctgcgctttc aacctttgca tcacatcctt
     7441 ttatgatgat aatgacggtt cttgcgttaa gcgcagtact ctcaagcttt atcggagccg
     7501 ttcccgtagt taccatattt atacccgttc ttgaaattat tattccctct gttcccaaag
     7561 gctatctgct ctggtgggct cttgcccttg gagcaaactt tgcaagcaac accacaattg
     7621 ttgcctctgc atgtaacatg gtggtggtag gacttttgga atccaacttt aaagaaaagg
     7681 taagcttttt tgagtttatg aaaaaaggaa tccctattac tatgatggga ctggctatat
     7741 cagcccttta tcttacagga ctgttttacc tgttctgagg aacagtttgg ttcataacag
     7801 tcaaggtttt aagtacttca atgcgtttgg atggatgttt ttcggctttt ttaatactcc
     7861 ttcttatttc gtctctgctg atgaaagaag gagtttttaa ttttgaaccg atatagtcgc
     7921 atattctgta aatacagtct acggtatctt tggttatctt atacttggca ttgaccttcc
     7981 acatgaatat atcaacattt tccatgccgg ttatcatgac catgctttcc tttatcaggg
     8041 agatgctcga ttccttatct gaaaaggtga aagctatttt cttctcgtgt gctccgttgt
     8101 caatgaaagt aaactcaaga gtcagttttt tttctgtcaa ccctttttcc catataagat
     8161 ccgccctgta ttcattggct ctgaagttat tactcataaa agcctcctat tcatataagc
     8221 ttactatttt ttgtgctgct acatctatta tacccctgtc tgttattttt atctgaggta
     8281 taacagcgag ttgtatgaaa gaaagtgtca tgaaaatatc gtttgagcac cccaaaagtt
     8341 caagacggtt cttcctttcc aagagttttg cagtaacctc ttttatagtc ctgtcgctca
     8401 tcaggcctgc tatagggaga ggaaattcaa atatgacgct tccgtttttc accatagcta
     8461 tcccaccgtt catttttttg acggtttgag ctgccaaaac aatatcagca tcgttcatgc
     8521 cacagacaca aagattgtga gaatcatgac ccacgcttgt ggccactgca ccctccttta
     8581 ttcctagtcc atgaatgaac cccaccgagt atccgctggc atgatggcgt tcgagaataa
     8641 ctacctttga tatatctctt gcagggtcag caaggatata tccgtttttt acagttggct
     8701 ctgttatcaa aggtgtggtg tacaagcttg ctttttcaat tcctataact tttatttttc
     8761 tgccggtatc ttttatcttt atgtcttgct cacagatctc aggaggatta aacgagtatc
     8821 ctgtcaattc ggtttttgag taaataccgt tcatgtctcc ggttagaact ccgtctttga
     8881 ccataagttt tgaattatgg aaaaccattt ttatattcag atcgtgcaga ttatcaacaa
     8941 ccagtaggtc ggctttatat ccgggagcaa cagctccgca gcttctgagg ttatagaact
     9001 tggccgtatt tatactagcc atccttatag ctactacagg atccaatccc tttgccacag
     9061 ctctttttac aagattattg atatgtcctt gttccgttat ctccagtgga tctctgtcat
     9121 cagtgcaaaa ggaaaagtac tggtgattgt aaaggtttac ggccggaata agagcgtcaa
     9181 agttcttggc cgcactgccc tcacgtataa aaatatgcat acccttgctt aacttctcaa
     9241 tagcttcttc ttttgttgta cattcatgat cagaccggat gaaagcattt atataaccgt
     9301 taagtttctt gccgctcagt cccggagcat ggccatctat ctttttatat ctatgtctta
     9361 aaatttctat ctttgttatg agttctggtt ctaaagacag cacaccgggg tagttcatta
     9421 cctctcccag tcccaccgtt cttttcagat gtgaatcaat aaaaccgatc atatcatccg
     9481 gacccagttt agcgcctgaa gtttccagag aagtggccgg aacacatgac ggaatagcaa
     9541 tataaacgtt cagagctatt ccttcggtcg aattgtttat atattcaagt ccgtctactc
     9601 ccatcacatt gactatttca tgcggatccg ctattactgt ggttgttccg ttaaggataa
     9661 caagctttgc aaagttcagc ggagagagca tagttgattc tatatgtaca tgggcatcta
     9721 taaagcccgg tatgacatac aggccatcca gatcagttat attatccgaa ttgtcgtatg
     9781 aacctactcc ggcaattctt ttgtcaaaaa gagctatatc ggaaacttct gtttctccgg
     9841 taaaaacatt cagaatttta gcatttttaa gaatacagtc agcgttcttt tcgcctaatg
     9901 ctacggggat catatccctt aatttcataa aagcctcctt tttctatctt aagtatctga
     9961 aaatccttta tggaaagtac agatatcatc ttttccaggc aactgctcct aacagtaatg
    10021 tataaggtta atccgctgtg aagaactttt ttaatcccca taaaaaaaag ctttccctca
    10081 agctcggcag gtccggcctc atctatgaag actggctgaa ctttcctgtt tataagatca
    10141 tggatacagc tgtcaaaaaa aatcagtgct tttttcgaga aagaaaacgg tccgaaacgg
    10201 catacttctt cccatttgtc atctataaac ttggggtaaa gtgcaaactc aactttagtt
    10261 tgattgtcta gccgataaag atcgtaacct aaaaatgttc cataacagaa cttttttctg
    10321 gttatgattc cgccgccttc tttattagaa ttgtataaat tttccataaa cgtagatttt
    10381 ccgctgttta tttcacccgt tactatgaag accatttgcg gcttaatccc ggcgcctgtt
    10441 taccatacat tgcagcctga atctgagcag ttaccgatac ggctatctcg gacggggttt
    10501 gcccgcctat atccagacct atgggagagt acagaacgga aagatcaaca tcagggtttt
    10561 ctttttttat tttacttact atcataccag cttttttttc actggcaagc attccgatgt
    10621 aaaaagcatc ggtttttttc attaaaagct cacgaaggac ttcgtaatct ttttcatgtg
    10681 agtaggtgca tataatataa atcccctttt tagggaattt atactctttt atagctgcta
    10741 tataatcttt tattatttta caagaaagct taacctccgg tacttgcaca tctgatcttt
    10801 cttcaaaaac acatacagaa taatcaagac gtgaaagaga cacaagaatt tcccttccga
    10861 catttccgat tccgaaaaga aaaatctcgg ccttaggacc aatatattca aaaaaaagtt
    10921 ttactgtccc gccgcacaac atatcaacag tctgactgtc caaccgctct ttttcgctgt
    10981 ccagagtgta tgtttttgca gcacattgtt tgttttcaag tacttcttta cagtctttca
    11041 gaacaagcat ttcaagtgga ccacctccta tggagcctgt tctttgcata gaagaagcaa
    11101 caaacatttt cgatccgatc tttcccgggc caaagccatc tttttcagtt acagttataa
    11161 gtactccgct ttttccgttt tcaatggaac tgattatctt ttcgtatatg tctgtcatgc
    11221 tgttcctcct tggccttttc ttcatcagat ctctttctaa aaatatcaag gcaaatataa
    11281 taatcatcaa ttgtgtcaac atccgaaacc acacctacgt catcggtttg caccggtaca
    11341 aaggtgtgat ttttcaatat atctctcaga ttgcgactgt ccggactcat aagaatctct
    11401 ggataaagag ctttacttat tattatagga tgtccttttt ttgatttgta taccggtatg
    11461 acgatacttc ctttggttac agcaagttta tgcacagttg tctgcctgaa gaggggatag
    11521 tctcctgggg ttatcaagag cttatcagaa ctgacatgct taagcccggt ttttagagat
    11581 gaaaacatcc ctttttcata actgtcattt ctgacaagct caaccttttt aaaatctttc
    11641 acaagctcct gcacctttcc gccgttataa ccgcatacca ctattacttt tgagcactcg
    11701 gcatatatgc tttttataca ctgctgtaaa agagtagact tgccgaacat aagctccatc
    11761 ttatacgatc cgcttctggt ggaaaggcct gctgccagga caagaccttc cagggaagat
    11821 gccataatct atattaccga aaatatcaac tgtgtggaga tagctatgaa aaaagcggtc
    11881 gaggcatata aaaagttcgg ttctgctttt ttagagctgt tttctttcct taatacataa
    11941 aaaatcatta aagcagtgct gattataatt atgttagcta ttgaatctgt tatccagaac
    12001 ttgtatacag ctgaggtgcc tttagaaagc agctccagtt tgagtgggaa tgttcttaga
    12061 atagaaagat atataagata tacagttctc catccaaagg tcatagcagc gatatccaag
    12121 ggattgagct ttctgccttt ctgaacagca aagaaaatga caagtaagac tgctatactt
    12181 tccataacaa tgcttatgct aggattaaca atcttaagaa tattgctgac aggaagaaaa
    12241 aagttgacaa gcttgatagc tgcggctgta atgccgcaaa tcagaacggt ttgcaactcc
    12301 gaactttttc tgtatgccct gaacatgatt gaaaaaccaa tcggaaacat tataaaagaa
    12361 gagattcccg gtataaaatc tgatacaaaa tgcaacagat accctaaagt agcttcaagc
    12421 atcccccaga cggaaccata aaaaatacct gcagataagt ttttcttcat cctagatcac
    12481 cctctctata tagtatttta gaagattaag cgaaactttt tttctgtaca ttgcattaga
    12541 acgctgatca tctataggtt ttaaatattt ttcataaagc tttgatatag aata
//
LOCUS       DAOSHR010000041        12564 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_4205_length_12564_cov_5.877049, whole genome shotgun sequence.
ACCESSION   DAOSHR010000041 DAOSHR010000000
VERSION     DAOSHR010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 12564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12564
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4205_length_12564_cov_5.877049"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1487
                     /locus_tag="PLU28_03610"
     CDS             <1..1487
                     /locus_tag="PLU28_03610"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOG34185.1"
                     /translation="EKLFQVYNLSEEIDKQTEELIREILKKSDLDLDSIIDKLNFQRK
                     INLSISAALAKKLSNIKTLYVQEKSIRKYAHVSLYHILGYVDIEGNPVMGLEKIHDKI
                     LRGQPGYKIVNLTPMGKIKSTVEETFSIPGDDIYLTIDLDIQIKAFEELENSGYPGSI
                     IVSNPQNGEIYALASFPTPDPNMFSRGLTNLEYRRLSNNTKKPFINRAISALYPPGSV
                     LKPFIALSSLEAGLDPNKTIFSDGRYELKNSKGQVIGSFTDWNLVGHGTTDLIKSLKE
                     SVNSYYYWLGEKYGIDYLKTKSDFYNITGKTFIDIPGESSGVFPDQKWKKDKFNEIWY
                     PGETLNAYIGQGYVLTTPIEILKFYNILAAKGKYYNFHTFLRSSNTLEKGSSIIYEVN
                     LTENYEIDHEYLALILKGLKEVTTPGGTAYDAFKNFPVITAAKTGTAEVSGGKKPHGW
                     FAGYMPADNPKYSIVVMVENVGYGSHVSAPIAKNVLDLIVKKGI"
     gene            complement(1458..2336)
                     /gene="sdaAA"
                     /locus_tag="PLU28_03615"
     CDS             complement(1458..2336)
                     /gene="sdaAA"
                     /locus_tag="PLU28_03615"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HOG34186.1"
                     /translation="MFSSFRELVDLSLKTGQPLAEVVKEWEMLENGTDPEYLSEQSLK
                     FVIQMIKTYKDKLNSDSVSLTGWTGFNTGLYNEYRNSNKSIFSNLISKSILASLATSE
                     SNACMGKIVACPTAGSSGVVPGVVVALHEEKGVDLKSLSESLIVAGAVGEFIRRKASL
                     SGAIAGCQAEIGSACAMASALVVYALGGSPQIVENAASLSLKFLMGLVCDPVGGFVEV
                     PCVKRNPAASVLAFVSAEMSMSGIKSLIPFDEITEAMGKVGRSLSEDLRETGKGGIAA
                     TKTARSLLNTFFNNKV"
     gene            2372..3211
                     /locus_tag="PLU28_03620"
     CDS             2372..3211
                     /locus_tag="PLU28_03620"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOG34187.1"
                     /translation="MYIIQYGSKEKIETITELVNEVFKDYVIPINWTKESFLLDMAEN
                     SIFLEHSQILYFGKEKLPIGFCITSAREKRARIDSFGIKNEYRKTGASYFLINNVFYD
                     LSKKGFSSLQLEVESSQTRAVDFYIKNGFKISRNLDSFILPEKTSCYKFNYSLNCERS
                     SIRQDILLKAKRSPNWQREITSVYSSKKDYFMNQIRSENKEIGKMLWGRTEDTTYIVD
                     IYQTDFTVKYSNIVQDAISYLSSFGKPLITVSLPQNDPLNSVLLALGSKIFLQQYEMV
                     LSL"
     gene            complement(3201..4844)
                     /locus_tag="PLU28_03625"
     CDS             complement(3201..4844)
                     /locus_tag="PLU28_03625"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG34188.1"
                     /translation="MRAVIIDGYVDEPAVLGVPPYISPYIRYSAGALIYRSIETDYIT
                     IDEIRKNDFFSSFDSYDYIIIIAGTTVPGHYFMATPISLYEIKKIFDYNSRPLKVLGG
                     PIARGYTLKGGSSALLLKDYLKESVDFVIPGDIEKFLFKYPVSDDYNLEERSDYELTD
                     MLSTLGAQIVTKHHMFPNVMLEIDVSRGCDRKDGFCSFCTEPLTHGAYRERKVNSVIS
                     EIKTLNSLGVRNFRFGRSANFLAYGSAVNKNLPNPALFEELYSEISGLSDVLHTDNAN
                     ADFIAKYEKECRKIIGTIAKYNTSGDILSFGVETFDPEVSQKNRLYNSNDSILKAVQI
                     VNEEGGWRDSDMIPKILPGINILYGLLGETKDTYEKNIEYLKMIYDKKFMLRRINVRQ
                     VMVFPGTFLYDNREKVSFDKKSFVRFKEFMTEYDSLMLQRVFPIGTVIRNILPQENRG
                     EISFGRQIGTYPILCGTYSDVKMDVFFDGKVVDYGSRSVTVIDNSKRMKDLSVRELSS
                     IKGIGRKTAEAVFLSQDSSVDKMILERKQKEILQSVFKG"
     gene            5004..5393
                     /locus_tag="PLU28_03630"
     CDS             5004..5393
                     /locus_tag="PLU28_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HOG34189.1"
                     /translation="MAKKIKSLGGRSLKPGISKKADMGQLLKEAQKAQVAMEAEMAEL
                     EEKLASQEVKASSGGGVVTVTAGGDLKIKNIDISPELQGESIDIIKDMIIAATNEALE
                     KASELKETESEKISQKYLSGFENMPNI"
     gene            5408..7375
                     /gene="tpiA"
                     /locus_tag="PLU28_03635"
     CDS             5408..7375
                     /gene="tpiA"
                     /locus_tag="PLU28_03635"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOG34190.1"
                     /translation="MNMLTVRDIDLNSKKVIMRVDFNVPVKNGKITDDTRITAALPTI
                     KYAIDHNAKVILLSHLGRPKNGPDQEFSLKPVAERLNELMPSLVSFVEDTRGESVNKA
                     VENLQQGHILLIENTRFEKGEEKNDTDLAKYWAGLADIHINDAFGTAHRAHSSNVGIA
                     SYIPSVAGFLMEKEIKFLSKVTYEPEHPYVVVLGGAKVSDKINVINHLLEKADKILIG
                     GAMMFTFLKAQGKNIGSSRYEADKVDFARELLEKAASKNVEIVLPVDSTVAKKLEAGT
                     ENRLVSIDDGIEEGWMGLDIGPETIKLFSEKLSGARTIIWNGPMGVFEIPDFSYGTKE
                     IAKRISSMTTKETITVVGGGDSAAAAEEFGMAQMFSHVSTGGGASLEYLEGITLPGIA
                     SISQKKNLGIRKFILAGNWKMNKTPSQAAEFACTIVPKLQEFKKITSVLCVPFTALER
                     VSEYVDRTHVFTGAQNMYHESSGAFTGEVSACMLKDISVDYVILGHSERRHIFHEGDE
                     LINQKVLKALDEGLIPILCVGEQLQDREDNLTFNIIELQVKKGLKNVSANDLQKVVIA
                     YEPVWAIGTGKVASPEQAQQVHEFIRELIAKLYSRQAADGITILYGGSVSESNYLGLF
                     LKKDIDGGLVGGASLKQSFINLASIMDSLIE"
     gene            7480..9183
                     /locus_tag="PLU28_03640"
     CDS             7480..9183
                     /locus_tag="PLU28_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34191.1"
                     /translation="MPNDTENNSSKNEFQDLLSEKKKENRFPMKKIYLLSTIGVLLLI
                     SALILFFVFSGKKHELTLSYSIPNTDITEDIQKIKVSISGKTSFVKEYGLNDPILIPK
                     LKEDNYSIEVSAINSKNYIVFSHKESIFIKDKTSKDFFLSREQVLYDLKPVKIGNDLS
                     VNLPAGFDKYLVYSKTNDKFEFSYETSINTVSLKGFSTLGTELKFSVLKDSRIYEFSD
                     ALSLSALIPPVPPKIISPENNELIISTNYFFVWQPASVDSLDLAYDLYLKQDDGTEFK
                     IAENIKDLMYEYKKLSPGKTYTLKVIAKNSFGQSTESNAVFRTASAEKDQEYLFAPSG
                     RMGVTIYEVKDPKKPTELSSIKLDGNVTDVIKKENYLYILRDQSGFTIADIKDLKNPV
                     IKKNVDVKDINGIKIVDNYLFTRVDGDKVYVYSLKDSYVSPVYQGETSIKYYGSSKPV
                     IQYVEKVVEKTATPVAIKVSIISSEYPINIDYKNKVYITEFSMIVQSDKAKNLISADS
                     ARVVETLRMILWKKTREDFSNSTRFGVILEQIRSGVNETFKLSESDGVKTVTMKISKF
                     E"
     gene            complement(9220..9645)
                     /locus_tag="PLU28_03645"
     CDS             complement(9220..9645)
                     /locus_tag="PLU28_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34192.1"
                     /translation="MYTYGLIVFSEDEEKAYAKALECSGRYSQGNISMAINFADDEEY
                     FNDTVTSLSSMYLDLKESSENLLSIYEKKLDKEDFLISQLHENLKRYESHLYSYPKEW
                     KIVIYNDGEVIHIDSSEILISFLNGKDKTHIYLFIIDAQ"
     gene            complement(9702..11192)
                     /locus_tag="PLU28_03650"
     CDS             complement(9702..11192)
                     /locus_tag="PLU28_03650"
                     /inference="COORDINATES: protein motif:HMM:NF006820.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HOG34193.1"
                     /translation="MQKKQITVKNPSRSYLIEKIIEKKEGNLLSNGTVNVDTRPFTGR
                     SPKDKYIVEDSFTKDKIWWGPINQPTSKETFISLRQELKAHLDSSNEVYIMNGYAGFD
                     DKYRVNVQLITCSPVQALAYKNLVIPADEEKFKPELTIYASPYFSADKAKYNTNSDVF
                     IIMDMSEKEVIIGKTVYPGEIKKSIFTYMNFALPLKGVFSMHCSANTDSENKHTALFF
                     GLSGTGKTTLSVVEDRLLIGDDEHAWHDEGIFNYEGGSYAKIINVSEDENPMIYHALK
                     YGVMTENVVFNDQRIADYSDQTITENTRACIPLDMLENVKSDGKASHPETIFFLSADA
                     FGILPPIAKLEEAQIYDYFLLGYTAKLAGTERGVKEPSLTFSECFGKPFIPLNPKVYA
                     QMLLEKVKRYGAEVYLVNTGWIGGAYGTGSRIKLRYTKRLVNAAINRELKDFEKFEIL
                     DLKIPKVCEGVPSEILNPSNVWTSKSEYEKKAKELYSKFKEHFEKF"
     gene            complement(11472..>12564)
                     /locus_tag="PLU28_03655"
     CDS             complement(11472..>12564)
                     /locus_tag="PLU28_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016695 - oxidoreductase activity, acting
                     on hydrogen as donor [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="[FeFe] hydrogenase, group A"
                     /protein_id="HOG34194.1"
                     /translation="EVWQEIDNPKKHVVVQTAPAVRVAIAEEFGYEPGTISTGKLVSA
                     LRALGFDKVFDTDFAADLTIMEEATEFLDRIKNGGYMPMLTSCCPGWVKFLEHQFPSL
                     VEMPSSAKSPQQMFGAIAKSFYAEKNNIDPKDMTVVSIMPCIAKKYEAGRPEINSSDE
                     YKDVDYVLTTRELARMIKEAGLNFDTLPEGQYDSPLGESTGAAVIFGRTGGVAEAALR
                     TAYEWITGKNLEAVEFEAIRGYEGVRVAEVKVGEMTVRVAVSHGLGNTRKLLEDIQAG
                     KLQLHLIEVMACPGGCVGGGGQPFHHGNFDIIKKRTEALNRADRNMPIRKSHENPAIK
                     ALYEEYLGKPASEKAHHLLHTHYLEREWL"
BASE COUNT         4077 a         2414 c         2197 g         3876 t
ORIGIN      
        1 atgaaaaact ctttcaagta tataatttga gtgaagaaat cgacaaacaa acagaggaac
       61 ttatacgtga aatacttaaa aaaagtgatc ttgacctgga ttcaataata gacaagctta
      121 acttccagcg aaagataaat ttaagcatta gtgctgccct tgcaaaaaaa ctctctaata
      181 ttaaaacctt gtatgtacaa gaaaaaagta tacggaagta tgcacatgta tctttatatc
      241 atatattagg ctatgttgat attgaaggaa atccagttat gggacttgaa aaaattcacg
      301 acaaaatact tcgtggacag cctggatata agatcgtaaa tcttactcct atgggaaaaa
      361 taaaaagtac tgttgaagaa actttttcaa ttccagggga tgatatttac cttacgattg
      421 atttggatat acagataaaa gcttttgaag aacttgaaaa ttcgggctat cctggatcca
      481 taattgtttc caatccgcaa aatggagaaa tctatgctct tgcaagcttt cctacacctg
      541 atccaaatat gttttccaga ggattaacaa atcttgaata tagaaggctc tcaaacaata
      601 caaaaaaacc atttataaac cgtgctatat cagctcttta tccgcctggc tcagtcttaa
      661 aaccttttat tgctctttcc tctttagaag cgggccttga tccaaacaag actatcttct
      721 ctgatggccg ttatgagctt aaaaactcta aaggacaggt aatcggatct tttacagact
      781 ggaatcttgt cggacacgga acaaccgatc taataaaatc tctcaaagaa tctgtaaact
      841 cttattatta ttggcttggc gaaaaatatg gaattgatta tctaaaaacg aaatctgact
      901 tttacaatat aacaggaaaa acttttattg atatcccggg tgagtcttcc ggagtttttc
      961 cggatcaaaa atggaaaaaa gataagttta acgaaatatg gtacccgggg gaaactctca
     1021 acgcctatat tggacaaggg tatgttctaa ctactccaat agagatcctt aaattttaca
     1081 atatccttgc tgcaaaggga aagtactaca actttcacac ttttctaaga tcatcaaaca
     1141 cattggaaaa aggttcatcg ataatttatg aagtgaatct tacagaaaat tacgaaatag
     1201 accatgaata cttagcgctc atactaaagg ggctgaaaga ggttacaact cctggtggaa
     1261 ctgcatacga tgcctttaaa aacttcccgg tgataaccgc agcaaaaacc ggaaccgctg
     1321 aagtttcggg aggaaagaaa ccacatggat ggtttgccgg atatatgcct gcggataacc
     1381 ctaaatattc gatagttgtt atggttgaaa atgtaggtta tggttcgcat gtatcagcac
     1441 ctattgcaaa aaatgtttta gaccttattg ttaaaaaagg tatttaaaag tgatctggct
     1501 gttttggttg cagcaatacc tccctttccg gtttccctga gatcttctga taatgatctg
     1561 cctacctttc ccatggcttc agttatctca tcgaacggta taaggctctt tatcccgctc
     1621 atagacattt ccgcagaaac aaatgcaagt actgaagctg ccggatttct tttcacacac
     1681 ggcacctcta caaatccgcc tactggatcg cataccaatc ccattaggaa tttaagactt
     1741 agggatgctg cattctcaac tatctgagga gaacctccta aagcataaac aacaagagct
     1801 gaagccatcg cacatgcaga accgatttca gcctggcatc cggctattgc accggaaagg
     1861 cttgcttttc ttcttatgaa ttcacctacg gccccagcca ctataagact ttcagaaagg
     1921 gatttaagat caacaccctt ttcttcatga agagcaacca ccacaccagg aaccacccca
     1981 ctggaaccag ctgtaggaca agcaactatc ttgcccatac aggcattgct ttcagaagtg
     2041 gcgagagatg caagaatgct tttggaaatt aagttactga aaatcgattt gttgctgttt
     2101 ctatactcat tgtataaccc ggtgttaaat ccagtccatc cggttaggct tacagaatca
     2161 gagttcagtt tgtctttata tgttttaatc atctgtatca cgaatttcaa cgactgctca
     2221 gagagatatt caggatcagt accattttca agcatctccc actcttttac tacctcagcc
     2281 agtggctgac cggtttttaa cgatagatcc acaagttccc gaaatgagct gaacatgctt
     2341 tttctccttt atattttttc ccggaggatt tatgtatata atacaatatg gttcaaaaga
     2401 aaaaattgaa actattacag aacttgttaa tgaggttttc aaagattatg ttatccctat
     2461 aaactggact aaagaatctt tcctgcttga tatggcagaa aattcaattt ttctggagca
     2521 ctctcagata ctatactttg gaaaagaaaa gttgcctatc gggttctgta ttacatctgc
     2581 tagagaaaaa agagcacgaa tagactcctt tggcataaaa aatgaataca gaaaaactgg
     2641 tgcttcttac tttcttataa ataatgtttt ttacgatctt tcaaaaaaag gattttcatc
     2701 actgcagctt gaagtcgaaa gcagtcaaac tcgtgcagtg gatttttata ttaaaaacgg
     2761 ttttaaaatc tctcgtaacc tagattcttt tatattacca gaaaaaacct catgctataa
     2821 attcaattac tccttgaact gtgaaagaag ttcaatacga caggacatcc tgctaaaagc
     2881 taaaagatct cctaactggc agagggaaat aacttccgtt tatagcagca aaaaagatta
     2941 cttcatgaac cagatacgat cggaaaataa agaaatagga aaaatgctgt ggggaagaac
     3001 agaagatacc acctatattg ttgatatata ccagactgac ttcactgtca agtatagtaa
     3061 tattgttcaa gatgctatat cctatctgag ctccttcgga aagcctttaa tcaccgtatc
     3121 tcttcctcag aatgatcctc ttaattctgt attgctggca ttaggctcta agatttttct
     3181 tcagcaatat gagatggttc tcagccttta aaaacacttt gaagaatctc tttctgtttc
     3241 ctttccaaaa tcattttatc aactgatgaa tcttggctga gaaatactgc ctcagcagtc
     3301 tttcttccta tgccttttat tgaacttaat tctcttacag aaagatcctt catgcgttta
     3361 gaattatcta tgacagtaac tgaacgcgac ccataatcaa caacttttcc gtcaaaaaaa
     3421 acatccattt ttacatctga atatgtaccg caaagtatag gataagttcc tatctgcctt
     3481 ccaaatgaaa tttcccctct gttttcctga ggaagtatat ttcttatcac cgttccaata
     3541 ggaaatactc tttgcagcat tagagaatca tattcagtca taaattcctt aaacctcaca
     3601 aaactttttt tatcaaaaga tactttttct ctgttatcat aaagaaaagt tccaggaaag
     3661 accataacct gccttacatt tatgcgtctg agcataaact ttttatcata aatcatcttt
     3721 aaatactcta tatttttctc atatgtatct ttagtctcac caagcagtcc gtacaagatg
     3781 ttgatacccg gaagaatctt gggaatcata tcgctatccc tccagcctcc ttcttcgttt
     3841 actatctgta ctgcctttaa tatgctgtca ttactgttgt aaagacggtt tttctgagat
     3901 acttcaggat caaaggtttc aactccgaat gagagaatat cacctgaggt attatactta
     3961 gctattgttc ctataatctt cctgcattcc ttttcatatt ttgctataaa atctgcattg
     4021 gcattatcag tatgaagaac atcggacaac cctgatattt ctgaataaag ctcttcaaaa
     4081 agagccggat tgggaagatt tttatttaca gcgcttccat aagccagaaa atttgcgctt
     4141 cttccgaatc tgaagtttct aactcccagc gaattaagtg tttttatttc cgaaataact
     4201 gaattaacct ttcgttcacg ataagcccca tgtgtcagag gctcagtaca gaatgaacaa
     4261 aaaccatcct ttctgtcgca gccgcgggat acgtctattt ccaacattac attaggaaac
     4321 atatgatgct tagttactat ctgtgctccc aaagtactta acatatcggt aagctcataa
     4381 tcagaccttt cttcaagatt ataatcatca gaaaccggat atttgaacaa gaacttttca
     4441 atatcgcccg ggattacaaa gtctaccgat tctttcagat aatctttaag taaaagtgca
     4501 ctactgcctc ctttaagagt ataacccctt gctatagggc ctccaagcac cttcaatggc
     4561 ctggaattat aatcaaatat cttttttatc tcataaagag agattggagt agccataaaa
     4621 taatgaccag gaacagttgt tccagctatg attattatgt aatcatatga atcaaaagaa
     4681 gaaaaaaaat catttttcct tatttcatca attgttatgt agtctgtttc tattgaacgg
     4741 taaatcaatg caccggcact gtatcttata tacggcgata tatatggcgg tacgcctaaa
     4801 acagcaggtt catcaacata accatcaatt ataactgcac gcaagtaccc acctcctgtt
     4861 tttcatctaa aaaattctat cactacttta ttacacccgc ttataaactt ttatactcta
     4921 attttactat tttttttgct ttttctatat aaaatggtat aattaaattt gtgtaatatt
     4981 taatttgaaa ggagagagaa tcgatggcca aaaaaattaa aagtcttggg ggaagatcct
     5041 taaaaccggg aatttctaaa aaagccgata tggggcagct gctaaaagaa gctcaaaaag
     5101 ctcaggtagc tatggaagca gaaatggcgg aactggaaga aaagcttgca tcacaggaag
     5161 ttaaggcaag cagcggaggc ggagtagtaa cagtaactgc cgggggcgat ctaaaaatta
     5221 aaaacattga tatatctcca gagcttcagg gcgagagtat tgatataatt aaagatatga
     5281 taatagccgc taccaatgaa gctttagaaa aagcttccga actgaaagaa accgaatctg
     5341 agaaaatttc tcaaaagtat ctgagcggtt ttgaaaatat gcctaatata taacggaggt
     5401 gagtttcatg aacatgctta cagtaaggga tattgatctg aactcaaaaa aagttattat
     5461 gcgggtggat tttaatgttc ccgtaaaaaa cggaaaaata acagatgata caagaataac
     5521 tgcggctctt ccgactatca aatacgctat agaccataat gcaaaggtta tacttctttc
     5581 acatcttgga agacccaaaa atggtcctga tcaggaattt tctctgaaac cggttgctga
     5641 aagactcaac gagcttatgc catctctggt ttctttcgtt gaagataccc gcggtgaaag
     5701 cgttaataaa gctgtggaga accttcagca aggacacata ctcttaattg aaaatactag
     5761 atttgaaaag ggtgaagaaa aaaatgatac ggatttagcc aagtattggg ccggtttggc
     5821 agatatccac ataaatgatg cttttggcac agcacacaga gctcattctt cgaatgttgg
     5881 aattgcaagt tatattccca gtgtcgccgg gtttctgatg gaaaaggaga taaaattcct
     5941 aagcaaggtc acttacgaac cggaacatcc atacgtagtt gttcttggag gagcaaaagt
     6001 atctgataaa attaatgtca taaaccatct tcttgaaaaa gcagataaaa tattgatagg
     6061 tggagccatg atgtttacct tcctcaaggc acagggcaaa aatataggtt cttccagata
     6121 cgaggctgat aaagttgatt ttgcaagaga actccttgag aaagcagctt ctaaaaatgt
     6181 ggaaatagta cttccagtcg attctactgt tgcaaaaaaa cttgaagcag gaacagaaaa
     6241 ccggcttgtt tcaatagatg atggaattga agaaggatgg atgggattag atatcggacc
     6301 agaaacaatt aaacttttct ctgaaaaact cagtggtgcc agaacaatta tatggaatgg
     6361 acctatggga gtttttgaaa tacctgattt ttcttatggt acaaaagaga ttgcaaaaag
     6421 aatatcctca atgacaacca aagaaactat aaccgttgtt gggggaggag attccgctgc
     6481 cgctgccgaa gagtttggaa tggcacagat gttctcccat gtatccacag gtggaggtgc
     6541 ttcgctggag tatcttgagg gaataaccct tcccggaatc gccagcatct ctcaaaaaaa
     6601 aaatctaggc attaggaaat tcattctcgc tggcaactgg aagatgaata aaacgccttc
     6661 gcaagctgct gagtttgctt gtactattgt tccaaaactt caggagttta aaaaaataac
     6721 ttccgtgcta tgcgtaccct ttaccgctct tgaaagagta tctgagtatg tggacagaac
     6781 tcatgttttc actggtgcgc aaaatatgta tcatgaatca agcggtgctt ttaccggtga
     6841 agtatcagct tgtatgctta aggatatatc ggttgattat gtgatactgg gacattctga
     6901 aagaagacat atcttccatg aaggtgacga actgataaat caaaaagttc ttaaagcact
     6961 tgatgaagga ctgattccca tattatgcgt cggcgaacag cttcaggatc gcgaagataa
     7021 tctcacattc aatataatag agcttcaggt aaaaaaaggc cttaaaaatg tttctgccaa
     7081 tgatttgcag aaggttgtta tcgcgtatga gccagtctgg gcaataggaa ctggaaaagt
     7141 agcctctcca gagcaagcac agcaagtcca tgagtttata cgtgagctta tcgcaaagtt
     7201 atattcaaga caagcagcag atggtattac catactttat ggcggaagtg taagtgagtc
     7261 aaattacctt ggcttgttct tgaaaaaaga tattgatgga ggtcttgttg gtggagcatc
     7321 actgaaacaa tcttttataa accttgcttc aataatggat tctttaatag aatgatatat
     7381 catgggtgga tttcaaatcc acccatttaa ttatcattaa tatcctgata atttagtttt
     7441 gtttaaaaat atgatataat ttcccatggg ggtgataaga tgcctaacga taccgaaaat
     7501 aattcaagca aaaatgaatt tcaggatctt ttgagcgaaa agaaaaaaga aaaccgtttt
     7561 ccaatgaaaa aaatctactt gctgagtaca ataggtgttt tgttgcttat ttcggcactt
     7621 atactttttt ttgtattttc cggcaagaag cacgaactga ctttaagtta ttccattcct
     7681 aacacagata taacagaaga cattcaaaag ataaaagtca gtatttctgg gaagacaagt
     7741 ttcgtaaaag agtacggact aaacgatcct attttaatac ccaaattaaa agaagataac
     7801 tactcgatcg aagtttctgc cataaactca aaaaactata tagttttttc gcacaaagaa
     7861 tcaattttta taaaagacaa aacaagcaaa gattttttcc tttcaagaga gcaggttctt
     7921 tatgacctta agcccgttaa gattggaaat gatctatcag taaatcttcc cgcaggattt
     7981 gacaaatatc ttgtatattc aaaaaccaac gataaatttg aattttcgta cgaaacatct
     8041 atcaataccg tatctttgaa aggattttca acccttggca cagagttaaa gttttcagtt
     8101 ttaaaagaca gccgaatata cgaattttcc gacgcattat ctctttcggc gcttataccc
     8161 cccgtacctc ccaaaataat atcgcctgaa aacaatgagc ttataataag tacgaattat
     8221 ttctttgtct ggcagcctgc aagcgttgat tcattagatc tagcttatga tttatacctt
     8281 aaacaagatg atggaactga gtttaaaatt gcagaaaata taaaggatct tatgtatgag
     8341 tataaaaagc tatctcctgg taaaacatat actttgaaag tgattgcaaa aaattctttc
     8401 ggacagtcca ctgaaagcaa tgctgtattt agaacggctt cggcagaaaa agaccaagag
     8461 tacctgtttg ctccaagcgg aaggatgggg gttacaatct atgaagtaaa agaccctaaa
     8521 aaacccacag aactttctag cataaagctt gacggtaatg taactgacgt tataaaaaaa
     8581 gaaaactatt tatatatatt aagagaccag tccggattca ccatagctga tataaaagac
     8641 cttaaaaatc cagtgatcaa aaaaaatgtt gatgtaaaag atataaacgg tataaagata
     8701 gtcgacaact atctttttac aagagtagac ggagataaag tttacgtata ctcacttaag
     8761 gactcatatg tatcgccggt ataccaggga gagacttcca taaagtatta cggctcttca
     8821 aaaccagtca tacagtatgt agagaaggtt gttgagaaaa cggctactcc agttgctatt
     8881 aaagtaagta ttatttcttc tgagtatccc ataaatatag actataaaaa taaagtctat
     8941 ataacagaat tttctatgat tgttcagagc gataaagcga aaaacctcat ttccgctgat
     9001 tcagcgagag ttgttgaaac gctgaggatg atcttatgga aaaagacacg tgaagacttt
     9061 tccaactcca caagattcgg ggtgattctt gaacagataa gaagcggtgt caatgaaaca
     9121 tttaaacttt cggaaagcga cggagtgaaa actgtaacca tgaaaatttc taaatttgaa
     9181 tagtcataaa aaaaacgaag gggtttcccc ttcgtttttt tattgtgcat caataataaa
     9241 aagatatata tgagttttgt cttttccatt tagaaaactt ataagtattt cagaactatc
     9301 gatatgaatt acttcaccat cattgtaaat aacaatcttc cattcttttg gatatgaata
     9361 tagatgactt tcataacgtt tcagattctc gtgaagctgt gaaataagaa aatcttcttt
     9421 atcaagcttt ttttcatata tagaaagaag gttttcagaa ctctctttta aatccagata
     9481 catagatgaa agactcgtta cggtgtcatt gaagtattcc tcatcatcag caaaatttat
     9541 tgccatagaa atatttccct gagagtatct gcctgaacac tcaagagctt ttgcatatgc
     9601 tttttcctca tcttccgaaa aaactattaa tccgtaagta tacatattct gtcctcccga
     9661 aaaaagctat ggagcaaagc tccatagcag gatataaatg attaaaattt ttcaaagtgt
     9721 tctttaaact tactatacag ttctttagcc tttttttcat actcagattt gctagtccag
     9781 acatttgaag gattaagaat ctcagaagga acaccttcac aaactttcgg tatcttaaga
     9841 tcaagtattt caaatttttc gaaatctttt aattctctgt taatagctgc attaaccagt
     9901 ctcttggtat atctgagttt tattcttgaa cccgtaccgt acgccccgcc tatccaacct
     9961 gtgttaacaa ggtatacctc agccccatag cgttttactt tctcaagaag catctgtgca
    10021 tataccttag gattcagtgg aataaaaggt tttccaaagc actctgaaaa cgtaagagaa
    10081 ggttctttta ctcctctttc tgttcctgca agtttagcgg tatatccaag taaaaaataa
    10141 tcataaatct gtgcttcctc aagcttggca ataggtggaa gtattccgaa tgcatcagca
    10201 gaaagaaaga atatagtctc cggatgcgag gcctttccgt ctgattttac attttcaagc
    10261 atatctagtg gaatacatgc acgtgtattc tcggtgatag tctggtcaga gtaatccgca
    10321 atcctttgat cattgaatac aacattttcg gtcattacac catatttcag tgcatgatag
    10381 atcatagggt tttcatcctc tgaaacgttt attatttttg catagctacc gccctcatag
    10441 ttgaatatac cttcatcatg ccatgcatgt tcgtcatcac ctataagaag cctatcttca
    10501 actactgaaa gagttgtttt tcctgttccc gaaagaccaa agaaaagagc tgtgtgctta
    10561 ttttcagaat cggtatttgc agaacaatgc atagaaaaaa ctcctttaag cggaagagca
    10621 aaattcatat atgtaaatat cgacttttta atttctccgg gataaacggt tttcccgatt
    10681 atgacttctt tttcactcat atccattatg atgaaaacat cggagttagt attatatttt
    10741 gctttatcag cagaaaaata aggtgaagca tatatggtaa gttcgggttt aaatttttct
    10801 tcatctgctg gtatgacaag gtttttatat gctagtgcct gaacaggcga acatgttata
    10861 agctgcacat tcaccctata cttatcatca aatccggcat atccattcat aatgtaaact
    10921 tcatttgaac tgtccagatg agccttgagc tcctgcctca aagatatgaa agtttcttta
    10981 ctagtaggct ggtttatagg cccccaccat attttatcct tggtaaaaga atcttctact
    11041 atatacttat ccttgggtga tctacccgta aacggtctgg tatcaacatt taccgttccg
    11101 tttgaaagaa ggttgccttc ttttttttca ataatctttt caataagata agatcttgac
    11161 gggtttttta ctgtaatctg ctttttctgc attttttcct ccttctaatc actataatat
    11221 taaagacagc cctcaagttt ttacttaaag gactatcagg aaagctaatt tttttcactt
    11281 atcacaatag atattaaaac atttttagac tcaaaaaaaa atcttagtaa taaggaactg
    11341 tctgtaaaaa aaagagttta ttattatata atgacaattt tgaacgttaa atgctttaat
    11401 gaaggtatct ttttaaagta agtttcaaaa tagaaaaagt gtcaggaaaa ctcctgacac
    11461 ttatcttttt attacagcca ctctctttca agataatgag tatgcagcaa atgatgagcc
    11521 ttttcacttg cgggtttacc aagatattcc tcataaagag cctttatcgc gggattttca
    11581 tgcgactttc ttataggcat gtttctgtct gctctgttca atgcctcggt tctctttttt
    11641 attatatcaa agttaccatg atggaaaggc tgtccgccgc cgcctacaca tccacctgga
    11701 caagccatta cttcgataag atgcagctgc agctttccgg cctgtatatc ttcaagaagt
    11761 tttcttgtat ttccgagtcc gtgagaaacc gctactctta cggtcatttc tcctactttt
    11821 acctctgcta ctcttacacc ttcgtatcct cttatagctt caaactccac tgcttcgaga
    11881 tttttgcctg ttatccattc gtaagcagtt cttaatgcag cttctgcgac tcctcctgtt
    11941 cttccgaata ttacagcagc acccgttgat tctcccaatg gcgaatcata ctgtccttca
    12001 ggtagagtat caaagtttaa tccagcctct ttaatcattc tggcaagctc tcttgtagta
    12061 agaacgtaat caacatcttt atactcatca ctggagttta tttccggtct gcctgcttca
    12121 tatttttttg caatacaagg cattatcgat acaactgtca tatctttagg atctatatta
    12181 tttttttctg cataaaaact tttggctatc gctccaaaca tctgttgggg agacttagcc
    12241 gatgatggca tttcgacaag tgacgggaat tgatgttcaa ggaacttgac ccatcccgga
    12301 caacaggatg ttaacattgg catatacccg ccatttttta ttctgtcaag aaactctgtt
    12361 gcttcttcca ttattgtaag gtcagctgca aaatcggtat caaatacttt atcaaatcca
    12421 agagccctta aagcgcttac aagcttgcct gttgatattg tacccggctc ataaccgaac
    12481 tcttcggcaa ttgccactct aactgccggt gccgtctgaa ctacaacatg tttcttagga
    12541 ttatctattt cctgccatac ctca
//
LOCUS       DAOSHR010000042        12490 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_4236_length_12490_cov_5.223000, whole genome shotgun sequence.
ACCESSION   DAOSHR010000042 DAOSHR010000000
VERSION     DAOSHR010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 12490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12490
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4236_length_12490_cov_5.223000"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="PLU28_03660"
     CDS             <1..609
                     /locus_tag="PLU28_03660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOG34195.1"
                     /translation="INGTFITSPDYKIDACDVVIFGEKVVSAWEDVYIVLYKPRSYIC
                     DRDSVRYPSVFSLIKEKIRKDLSVAGRLDADVHGLVILSTDGEMVHKIISPKKNVYKK
                     YEAQFSGSLTQQSLEMLRNGVEFDDFTSKPAFVTVLSDNLMQIEISEGKYHQVKRMMA
                     AAGLSLLDLKRTAIGAITLGKLLPGEYEYITKQEIYDKLLYK"
     gene            624..2459
                     /locus_tag="PLU28_03665"
     CDS             624..2459
                     /locus_tag="PLU28_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOG34196.1"
                     /translation="MLVRLDNVSHNFSSQDLFFDVDLTVYEGDRIALIGQNGSGKTTL
                     FNIISGEVEPIEGNVIRNNSLNIRFLKQFRADNASENLVQFLRASIVEEIDDIVINKS
                     IRSILIGLGFEETQWERQVSTLSGGELTRLALGRTLVGLNNNMDNFMLEKKIPLLLLD
                     EPTNHLDLYSIKWLISYLRSLRVTMITISHDRNFIKELSNKFWEINNYKVWDFSGDYE
                     TYMNQRENLLLSIQARKKNLEREMERLERMIDRYRKWATEKMVRQAIIRERSLEKLKK
                     EYQEIESMQELKSIKIKIPEPEKTGYVVLRVEKLSFAYEENSILESISFEVGEGQKIS
                     VIGKNGCGKTTLLNILLGKLEQSSGTYQWGHNIKIGYLDQVISSFNQKLDLISEIWKL
                     VPDWKDFEVRKYLGRFGFEGENVFKTIGDLSGGELTRIALSKLILGKPNVLILDEPTN
                     HLDILTVQTLEETLKEFSGAIILVSHDERLLRNISDKFVFIKDGRSETSDNLDSFLQD
                     IKEDTFKLDKNKNKDTQSWEDSRKLKNRRKSLENKVQLLGEKLEDLLYKLDKVEKDMV
                     TNGNNYQKVIELMKIKSELEQELVSTEEQEKITAEELKKVVSAEV"
     assembly_gap    2499..2598
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2616..4550
                     /locus_tag="PLU28_03670"
     CDS             2616..4550
                     /locus_tag="PLU28_03670"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HOG34197.1"
                     /translation="MLRLFSDQSERYLSPVQPKSNEKVSIRLRVPKSLGPTEGNVYFL
                     PQKNPGYYKHAQMELIMETKHFKFFEAVFVMPERIIRYHFEVRVLNSEKSIFYDAVGV
                     VKNRAVEDFILIPDVRYPEWAKGAIYYQIFVDRFYNGDKSNDVTDGEYIYDNFPVSSK
                     KWSDMPQKGNGHREFYGGDILGVMKKLDYIKALGAEVIYLNPVFVSPSPHKYDTQDYN
                     SIDPHFGVITENHGSYREITKSRKNLDASNRLFSDFMKKCHENNIKVVLDGVFNHCGS
                     ENEWLDNPQYRDFFYWNKENEYEGWWGYQTLPKLNYGTLKVWQAISGIGRRWVEEPYC
                     IDGWRLDVASDLGKSKYQNISFWRYFSKLVKKSNPDAVVFAEMYSSPKEWIESKAWDS
                     VMNYNGFMEPVSFYLTGMEKHNEFFSPELYLNAGKFIVSVIKAYAQLPMQSRFIALNQ
                     LSNHDHSRWMTRTTKVFGALGEKSHLQACEGVDIEVFKTGLIMLYTFPGAKGLYYGDE
                     IGMSGWTDPDNRRPFPWDSMTDENISLLEFCKKINAFYLQNTFLRTATFEFTYYTEHS
                     LVYICYSKDDFVVVAVNPSQKTEDLQIDLSVIDVENIYLVSEISNNGKKYSLKYEGEP
                     VTFSLDERTSVIFKKSKSML"
     gene            4594..4893
                     /locus_tag="PLU28_03675"
     CDS             4594..4893
                     /locus_tag="PLU28_03675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOG34198.1"
                     /translation="MKKEIMGNTGKVYLEGRVDITNSADLKNVLSDLKASGVKRVIID
                     MSKLEYIDSSGLGRIFYFFADFKKDGGEMEIHNINSPNVKKVIEIVKLDKIIATK"
     gene            complement(4961..5590)
                     /locus_tag="PLU28_03680"
     CDS             complement(4961..5590)
                     /locus_tag="PLU28_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34199.1"
                     /translation="MAEKEKKSLTVKITGVLPSINVIETSDYKDFDSLVKEYINKTGG
                     VLTKIIINEKEVPMKFYDEIKTSFFEGGEVIELEFSDRNYVLMNLFESGKDYINKIKQ
                     NLDSVSRQALLDSQQSHEMLQAVAEGFQAMINIIMIAENQMFFGKAIHTPEQLDKIKK
                     VVSQIVSSQEKKDSVDLSDIIDFDLPQTLETFKEIFENAEKEMKKEKSN"
     gene            complement(5606..6916)
                     /locus_tag="PLU28_03685"
     CDS             complement(5606..6916)
                     /locus_tag="PLU28_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34200.1"
                     /translation="MAFLKGTSGDYISVRPVGIKKIVINDNNEDILERYSYEVEVKSG
                     NYIWTNNEFSAIKSNSIDKLSIDFEYNPLVLTLLRINMFLKSSKKNMSSVLCADIDNQ
                     TAQIISKEALLFTFSESSEVVIVNIKKAGRFYEHNIIFSNNDYQNYFNISLKVDKKGL
                     VSFYKDLLIETNNFRLSTLPLVIDSLTDMKDLIDKRIKIIPYDEIKEIKDEYFDIYGL
                     DLSEFVNEIDASEDLKEFVDYLYFMKENGYDEKSSDDPQDPDEQDEDEVEVSFIIDDI
                     EDLESEIEQILENAVPDTVQHDIKAYDADMDFNDILAGIEEDFMEIMDNFFFSAQEEE
                     KEEIIEDLIEVKDKIYTYSKKNDTKEEFEKKLIVDLINAYGIEEVNKNIKYVKEIFSV
                     IEIDGKLSNYYDISEEKKINEYKNWYRKIFSKKGTAVSGLISKN"
     gene            complement(6931..7845)
                     /locus_tag="PLU28_03690"
     CDS             complement(6931..7845)
                     /locus_tag="PLU28_03690"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HOG34201.1"
                     /translation="MILSLTGTLLLSILLTFAMIKLAVRLNIVDRPDSFLKPHARTTP
                     YMGGIGVFFSVIFFYITDIHFVIPSIILLILGLYDDIKSLNPKIRILVEFLTTSLFVV
                     LYSSETNPFWLILLVLTGVALINSVNMIDGMDGVCTGTVLIALASFYFLTGDWRILFF
                     IAALSGFLIFNFNPAKIFLGDAGSYFLGFSLFFYLVQSFRQTHFEGYIKGIILCGLFF
                     TDLFFAVLRRMANGKSPFSGDRDHLYDKLSRLKGVSVKLSSLIMYVFALIFGVIGNVS
                     WNNLFFGVIISLVVFLYIGIKYRLYTYK"
     gene            complement(7842..8840)
                     /locus_tag="PLU28_03695"
     CDS             complement(7842..8840)
                     /locus_tag="PLU28_03695"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOG34202.1"
                     /translation="MNNPTEAKISVVLICRNEKKHLLGVIMSLLNQSLKPYEIIVADG
                     MSEDGSYEYLLELSVAHPELKPCKNHLTYAPYGLNIGIKQAKGDYIMIAGAHTLYNKE
                     YLKQTVCFLSENPAAQAAGGVATAFSVQGNTLQKSFCAVYSSVFGVGGSLHRYAKEIT
                     SADTVAYAVYRREVFQKYGYFNESLIRNQDIEFNYRITKTGGKIYVLPIDNLYKVPEG
                     FIKFVLKNFSNGYWNYITLKISKKGIGFRHFVPFLFVLYILLCIITGVFVPLKTSLII
                     CAPLFLYTGMDILFSFIQARIAKEPLQFFFNLFIFPILHISYGTGTLVSIIRGGEK"
     gene            complement(8824..10674)
                     /locus_tag="PLU28_03700"
     CDS             complement(8824..10674)
                     /locus_tag="PLU28_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HOG34203.1"
                     /translation="MKYMAKRKLTLVLIDYALFVCAYVISMFIRFQFDFAEMSKYISP
                     IFLYALIMVITLYLSRIYESIWRFAALPQLLPVFYSAGLGFLVNLFVFEFLRRYVTSF
                     FILPFTVNAITSLTGVILIAWSRIYWFSKTNKSSVKETDNNILIIGAGEIGVQILDEF
                     ERRPGEGTVAGFLDYDYDKIGRSIRGYKVFDVPENTMKYIDLLKIKEVIVAISNITSA
                     DLRKLLGFIDTKKVRVRILPGMFEVLNSKASLGILREVDVTDLLGRKEVKVDLKEISE
                     YIRGKNILITGAGGSIGSEICRQAAVCAPKELYIMGRGENSIFNIKNELNEKFPQVKI
                     TEIICDITDEKRLRKIFERFSFDVVFHAAAHKHVPLMEKNPTEAFKVNVLGTYYLASL
                     AVKYKIKKFIFISTDKAIKPTSIMGCSKRFAEEVIRSFSNKTDTVFGMVRFGNVVGSR
                     GSVIPIFKNQIAKGGPVKVTDPRMKRYFMTIPEAVTLVLQCMIFSSGGEVFILDMGEP
                     ILIDSLARELIRLSGLVPDQDIKIEYTGIRPGEKLYEELLLNDEGYLKTNNERIFVVN
                     ANHSLSEQEVEVMVGKIRQFVADSDFEGINGLIRTYVPDAGAVIKVEQSY"
     gene            complement(10658..11917)
                     /locus_tag="PLU28_03705"
     CDS             complement(10658..11917)
                     /locus_tag="PLU28_03705"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOG34204.1"
                     /translation="MLIPLSNPDITEKEKSTVMQVLDSPDLALGPYLKKFEDSFSSYT
                     GLKYALGVNSGTSALHVILRSMGFAKEHGMLTTPFTFVASANVAIYEHGDPVFIDIDP
                     YNYNISIDKLNRFFEGTYRPSKNHIKTDFLMAVDVFSLPLDWDKIRIPSEIKVIEDSC
                     EALGAEYKNKKAGTFGLAGAFAFYPNKQITTGEGGIIVTDDEGIYKAVKSMSNQGRGE
                     SGAWLEHVRLGYNYRMDEMSAALGYCQMTRLDEILDKRKSAADRYRKLFEGNSFFVCP
                     TVPDYVTRESWFVYVVRINIAWISKLLGLPNWISTYQMPDIIEHKNREEWYTYLKKAR
                     TILDSLLEKLSKKGVQARNYFSPVHLQKFYKDLYGYEEGDFPYTELTSCLTVAIPFFS
                     NITLEQQEYVVKSITESALEIQNEVHG"
     gene            complement(11927..>12490)
                     /locus_tag="PLU28_03710"
     CDS             complement(11927..>12490)
                     /locus_tag="PLU28_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34205.1"
                     /translation="PQVLELSTQELESFVLPQWQVYTFIGCGFMVMVSYVLMINRIVK
                     DQWMLQESEIRYGIVKDRIINYRDGTFAIILRIVTFNFYEWYLLYVLLRETAYHYFED
                     GTASDKYTQAIKSNQTIDPQIIDPPEDSYDLLLKELSTIGDEERFSIIFRKVTAMEKE
                     KALLILNRLLQENKITQKEYSRIEEFI"
BASE COUNT         3927 a         2249 c         2311 g         3903 t
ORIGIN      
        1 atcaacggaa catttataac gtccccagac tataagattg acgcatgtga tgtggttata
       61 ttcggggaaa aagttgtttc tgcctgggag gatgtttata tagtccttta caaacccaga
      121 agttatatct gtgaccgtga ttctgtaagg tatccttcgg ttttttcact tataaaggaa
      181 aaaatacgga aagatctttc tgtcgcaggg agacttgatg cagatgttca tggacttgta
      241 attctttcca ctgacggaga gatggttcat aaaataatat ctcccaagaa gaatgtgtat
      301 aaaaagtatg aagctcagtt ctctggaagt ttaactcagc agtccctcga aatgctcaga
      361 aatggagtgg aatttgacga cttcacatct aaacccgctt ttgttacagt actttcagat
      421 aatcttatgc agatagagat aagcgaggga aagtaccatc aggtaaagag aatgatggcc
      481 gctgccggtc tatcactgct agatctgaaa agaacagcta ttggagcaat tactttagga
      541 aagcttcttc cgggagagta tgaatatatt acaaaacagg agatctacga taaattgcta
      601 tataaataag gagttgattt aaaatgctgg tcagactcga taatgtttcc cacaacttca
      661 gcagtcagga tctttttttt gatgttgatc tgactgtata tgaaggtgac cgcatagccc
      721 tgatcgggca gaatggttcg ggaaaaacca ccctttttaa cataatttca ggagaggttg
      781 aacctataga aggtaatgtt atacgtaaca acagtttaaa tattcgtttt ttgaagcagt
      841 tcagggcaga taatgcttcc gaaaacctgg ttcagtttct tagagcaagt attgttgaag
      901 aaatagacga tatagttata aataagtcta tacgctcaat ccttattgga ttgggttttg
      961 aagaaactca gtgggaaaga caggtttcaa ccttgagcgg aggggaactc acaaggcttg
     1021 cattaggaag aactcttgta ggcttgaaca ataatatgga taattttatg cttgaaaaaa
     1081 aaattccctt gctcctttta gatgaaccaa ccaatcatct tgacctctac tctataaaat
     1141 ggcttatttc ttatctaaga tctttgagag taactatgat taccatatcg catgacagaa
     1201 actttattaa agagctttct aataagtttt gggaaataaa caactataag gtttgggatt
     1261 tttctggtga ctacgaaaca tatatgaatc aacgcgagaa tcttcttctt tctatacaag
     1321 ccagaaaaaa gaatcttgaa cgagagatgg aacggttgga gaggatgata gaccgttaca
     1381 ggaaatgggc aaccgagaag atggtaaggc aggcaataat ccgtgaaaga tctcttgaaa
     1441 aactcaaaaa agagtatcag gaaatagagt caatgcagga gctaaagtca ataaagataa
     1501 agatacccga acccgaaaaa acaggatatg ttgttctgag agttgaaaag cttagttttg
     1561 catatgaaga aaattccatc cttgaaagta tatcttttga ggtcggcgaa ggtcagaaga
     1621 tttctgttat aggtaagaac ggctgcggta aaactacgct tcttaacata cttcttggca
     1681 aactggagca aagctctggc acatatcaat ggggacataa tattaaaatt ggatatctag
     1741 atcaggtaat atcttccttc aatcaaaaac ttgatcttat atcagagata tggaagttgg
     1801 ttcctgattg gaaggacttt gaagtcagaa agtatctcgg aaggtttggt tttgaaggag
     1861 agaatgtatt caaaacaata ggggatcttt cgggaggaga gcttacaagg atcgctcttt
     1921 caaagcttat tcttgggaag ccaaacgttc ttatacttga tgaaccaacc aaccatcttg
     1981 acattcttac agtacagact ttggaagaaa cactcaagga gttttcagga gcaataattc
     2041 tggtctcgca cgatgaaaga ctgctcagga acatctcaga taagtttgtt tttataaaag
     2101 acggaagatc agagacttct gataatcttg actcctttct tcaggatata aaagaggata
     2161 cctttaagct tgataaaaat aaaaataagg atacccagtc atgggaggat tcaagaaagc
     2221 ttaagaatag aagaaagagc ctggaaaaca aggttcagct gttgggtgag aaacttgaag
     2281 atctgttgta taaactggat aaagtagaaa aggatatggt tacgaacgga aataactatc
     2341 agaaagtcat tgaacttatg aaaataaagt ctgaactgga acaggaactt gtatctactg
     2401 aggaacagga aaagattaca gccgaagaat tgaaaaaagt agtttctgcg gaggtataag
     2461 atgctcagat tattttctga tcagtctgaa aggtatctnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnntt ctgcggaggt ataagatgct cagattattt tctgatcagt
     2641 ctgaaaggta tctttcaccg gtacagccaa aaagtaatga gaaagtaagt ataaggctca
     2701 gagttccgaa aagccttggt cccacagaag gaaatgttta ctttttacca cagaagaacc
     2761 ctggctatta taaacatgct cagatggagc ttataatgga aaccaaacat tttaagttct
     2821 ttgaagctgt ttttgtcatg cctgaaagga taataaggta tcattttgaa gtaagggtac
     2881 tcaatagtga aaaatctata ttctatgatg ctgttggagt tgtaaaaaac agagctgtcg
     2941 aggacttcat acttattccg gatgtacggt atcctgagtg ggccaaagga gccatttact
     3001 atcagatctt tgtggatagg ttttataatg gtgataagag caacgatgtt accgatggag
     3061 agtatatata tgacaacttt ccggtatcaa gtaaaaaatg gagtgatatg cctcaaaaag
     3121 gcaacggaca cagggagttc tacggaggag acattcttgg tgtaatgaaa aaacttgatt
     3181 acatcaaagc tcttggagcc gaggttatct atcttaatcc ggtatttgtt tctcccagcc
     3241 ctcataaata tgatacccaa gattataaca gcattgatcc ccattttggg gttattaccg
     3301 aaaatcatgg ctcataccgc gagataacta aaagcaggaa gaatcttgat gcaagcaaca
     3361 gattgttttc tgattttatg aaaaaatgcc atgaaaacaa tattaaagtt gttcttgacg
     3421 gtgtttttaa ccattgtggc tctgaaaacg aatggcttga taatcctcaa tacagggatt
     3481 ttttttattg gaataaagaa aatgaatatg aagggtggtg gggttaccaa acccttccta
     3541 agcttaatta tggtacactt aaagtgtggc aggcgatatc gggtataggc cgcagatggg
     3601 tggaagagcc ttactgtata gatggctggc ggcttgatgt agcatctgat ttaggtaaga
     3661 gcaagtacca gaatatttca ttctggaggt actttagtaa gctggtaaag aaaagtaatc
     3721 cggatgctgt tgtttttgca gaaatgtact cttcaccgaa agaatggatt gaatctaagg
     3781 catgggattc ggtgatgaac tataacggct tcatggaacc ggtcagcttt tatcttactg
     3841 ggatggaaaa gcataatgag tttttcagtc cggagctcta tcttaatgcc ggaaaattta
     3901 tagtctctgt aataaaagca tacgcacagc ttccgatgca aagcagattt attgcattga
     3961 atcagctaag caaccatgat cattccaggt ggatgacacg tacaaccaag gtctttggag
     4021 ctcttggaga gaagagtcat ttacaggctt gtgaaggtgt agatattgaa gtattcaaaa
     4081 ctggattgat catgctttat acatttcctg gagccaaagg gctttactac ggagacgaaa
     4141 taggtatgtc cggctggaca gatcctgaca acagaagacc tttcccgtgg gatagtatga
     4201 cggatgaaaa tatatctttg cttgagttct gtaaaaaaat aaacgctttt tacctccaaa
     4261 atactttttt gcgtacggca acgtttgaat ttacttacta tacagaacat tctctggtgt
     4321 atatatgcta tagcaaggat gattttgtgg ttgtggcggt taatccttca caaaaaactg
     4381 aagatcttca gatagatctt tctgtgattg atgttgagaa tatttatctg gtatctgaaa
     4441 tctccaacaa cggaaagaag tattctttaa aatatgaagg agaacctgtt actttttctt
     4501 tagatgaaag aacatctgtt atttttaaaa aatcaaaatc tatgttataa tttaaagtaa
     4561 tttaaaagta ttttctaaaa ggaggtaact ctgatgaaaa aagagataat gggtaatact
     4621 ggaaaggttt accttgaagg cagagtagac ataactaatt cagccgatct gaagaatgtt
     4681 ctttctgatc ttaaagcatc gggtgtaaag agagtaataa tagatatgag caagctggag
     4741 tatattgaca gcagcggact gggaagaata ttttatttct ttgcggactt caagaaggat
     4801 ggcggtgaaa tggagattca taacatcaat agtccgaatg taaaaaaagt tatagagata
     4861 gtaaaactgg acaagataat agccacaaaa taaaaataaa gttttagtta tataaacaaa
     4921 ccacggttat atcttaaccg tggtttgttt atacttaagc ttaattgctc ttttcttttt
     4981 tcatttcttt ttcggcattt tcaaatattt ccttgaaggt ttcaagagtc tgtggaagat
     5041 caaagtctat tatgtccgaa agatctacag aatctttttt ttcctgggag ctaactatct
     5101 gggatacaac tttttttatc ttatctagct gttctggagt atggatagcc ttgccgaaaa
     5161 acatctgatt ctctgcaatc attattatat ttatcattgc ctgaaaacct tcagctacag
     5221 cctgaagcat ctcatgcgac tgctggctgt ccagaagagc ctgccgggaa acactgtcaa
     5281 ggttctgttt aattttattt atatagtctt ttccgctttc aaaaaggttc ataagcacat
     5341 aatttctgtc tgaaaattca agttcgatta cttctccacc ctcgaaaaag gatgttttta
     5401 tttcatcata aaacttcata ggaacttctt tttcatttat tatgatcttg gttagcacgc
     5461 ctccggtttt atttatatat tctttaacca gactgtcgaa atccttataa tctgaagttt
     5521 ctattacatt gatacttggc aggactcctg ttattttgac ggtaagtgat tttttttctt
     5581 tttcagccat atctgacctc caaaattagt ttttgcttat aagtccgctc acagcggttc
     5641 cttttttact gaaaattttt ctgtaccaat tcttatattc atttattttc ttttcttccg
     5701 agatatcgta ataattcgat agttttccgt ctatttctat gactgaaaaa atttctttaa
     5761 catacttaat gttcttattt acttcttcta tgccataagc atttataaga tcaacgatga
     5821 gctttttttc aaactcttct ttggtatcgt tcttcttact gtaggtatat attttatctt
     5881 ttacttcaat taaatcctct ataatttctt ctttttcctc ttcttgagct gagaaaaaga
     5941 aattatccat tatttccatg aaatcttctt ctattccggc aagaatatcg ttaaaatcca
     6001 tatctgcatc ataagccttg atatcatgct gaacagtatc tggtacagca ttttcgagta
     6061 tctgttcaat ctctgattcc aaatcttcga tgtcgtctat tatgaagctc acctctacct
     6121 cgtcttcatc ctgttcatcg gggtcttgcg ggtcatcact gcttttttcg tcatatccgt
     6181 tttctttcat aaagtaaaga taatcaacga actctttcag atcttccgag gcatcaattt
     6241 catttacaaa ctcagacaga tcaagaccgt atatatcgaa atactcatct tttatctcct
     6301 ttatttcatc gtacggaata atctttattc ttttatctat aagatccttc atatcagtaa
     6361 gactgtcaat gacaagcgga agagtgctca atctgaaatt gttggtttct atcaaaagat
     6421 ctttgtaaaa acttacaagt ccttttttat ctactttcaa ggatatatta aaatagttct
     6481 gatagtcgtt gtttgaaaaa attatattgt gttcgtaaaa tcttccggct tttttaatat
     6541 tgactatcac cacctcagag ctttcagaaa aagtaaacag aagagcttcc ttggaaatta
     6601 tctgggcggt ctgattgtct atgtctgcgc acaagacaga agacatattc tttttggatg
     6661 atttcaaaaa catatttatt ctcagcagag tgagaacaag ggggttgtat tcaaaatcta
     6721 tggaaagttt gtctatgctg ttactcttaa tagccgagaa ctcattgtta gtccatatat
     6781 agtttccgga ttttacctct acttcgtatg aatacctctc aaggatatct tcgttgttat
     6841 cgtttataac aatttttttt atcccgacag gacggacaga gatataatct ccgctggttc
     6901 cttttaaaaa ggccaaaagc cacacctcac ttacttatat gtataaagtc tatattttat
     6961 accgatatat aaaaatacta ccaaagaaat tataacacca aaaaaaagat tattccaact
     7021 aacattccct attactccaa aaattaaagc aaatacatac attattaacg aacttaattt
     7081 taccgaaaca ccttttaatc ttgaaagttt atcgtaaagg tgatcgcggt caccgctgaa
     7141 cggcgatttt ccgttagcca tacgccggag tacagcaaaa aaaagatcgg tgaagaaaag
     7201 cccgcagagt atgattcctt tgatgtagcc ttcaaaatga gtttgcctga acgactggac
     7261 cagatagaaa aatagtgaaa aacctaaaaa gtatgatcct gcatccccga gaaatatttt
     7321 tgcaggattg aagttgaaaa taagaaatcc agatagtgcg gctataaaaa acagaattct
     7381 ccagtcaccc gttaaaaagt agaaggaagc aagggctatc aggactgttc ctgtacatac
     7441 tccatccata ccgtcgatca tattgacaga atttataaga gccacgccgg ttaaaacaag
     7501 aagtataagc cagaatggat ttgtttctga tgagtataaa acaacgaaaa gagaagtagt
     7561 caaaaactca accaagattc ttattttagg attcaggctc tttatgtcat catacagtcc
     7621 cagtatcaaa agaatgatcg acggaatgac aaaatgaata tctgttatat aaaaaaatat
     7681 aacagaaaaa aacacgccga ttcctcccat ataaggggtt gtccttgcat gcggttttag
     7741 aaagctgtcc ggacggtcca ctatatttaa cctgactgca agttttatca tagcaaaagt
     7801 aagaagtata gaaaggagta atgttccggt taaggacaaa atcatttttc tcctcctctg
     7861 ataatactca cgagggttcc tgttccataa gaaatatgaa gaataggaaa gataaataaa
     7921 ttaaagaaaa actgtaaagg ttcttttgca attcgagcct gaatgaaaga aaaaagtata
     7981 tccatgcccg tatagagaaa taatggtgca cagataataa gagaagtttt taatgggaca
     8041 aaaactcctg ttattataca taaaagtata tacagtacaa ataaaaatgg aacaaaatgt
     8101 ctaaatccga ttcctttctt tgaaattttt aaggtaatat aattccagta tccattggaa
     8161 aaatttttta aaacgaactt gataaaccct tccggaacct tgtataaatt atctataggc
     8221 aggacgtata tctttcctcc ggttttggtt atgcggtaat taaactctat atcctgatta
     8281 cgaataagcg attcgttgaa gtaaccgtat ttctggaaaa cttccctgcg gtatacagca
     8341 taagcgacag tgtcggctga agttatctct tttgcatatc tgtgcagtga ccctcccact
     8401 ccgaatacac ttgagtatac agcacaaaag gatttttgca gggtattgcc ttgaacagaa
     8461 aaagccgttg ccacacctcc ggcggcttgt gctgctggat tttcagaaag aaaacaaacc
     8521 gtttgcttta ggtactcttt attataaaga gtatgtgctc cggcaatcat aatataatca
     8581 cccttagctt gtttgatgcc gatattgaga ccgtatggag catacgttaa atgattcttg
     8641 catggtttta actcagggtg agctacactt aactccagaa gatattcata gcttccatct
     8701 tctgacattc cgtcggcaac aattatttca taaggtttaa gactttgatt gagtaaggac
     8761 attataaccc caagaagatg ctttttctcg tttcggcata taagtacaac ggatattttg
     8821 gcttcagtag gattgttcaa cttttatcac cgccccggca tccggaacat aagttcgtat
     8881 aagaccgttt ataccttcga aatcactatc ggcaacaaac tgtcttatct tacctaccat
     8941 aacttcaact tcctgttcgc ttaggctatg atttgcgttt acaacaaata ttctttcatt
     9001 gttggtcttg aggtaaccct catcattaag gagaagctcc tcataaagct tttcgcctgg
     9061 tcttatgccg gtatactcaa tctttatgtc ctggtcagga accagaccgc tcagacgtat
     9121 gagctctctg gcaaggctat ctatcaatat aggttctccc atgtcgagta taaaaacttc
     9181 tccgcccgaa gaaaatatca tacactgtaa tactagtgta actgcctctg gaatggtcat
     9241 aaagtacctt ttcattcgtg gatcggtaac ttttaccggt cctcccttgg caatctgatt
     9301 tttgaatata ggtatcacgc ttcctctgct tccaacaaca tttccaaaac gtaccatacc
     9361 aaaaacagta tcagttttgt tggaaaacga tctgattact tcttccgcaa aacgtttaga
     9421 gcagcccatt atagacgtag gctttatagc tttgtctgtg gagatgaaga taaatttttt
     9481 tattttgtat tttactgcaa ggctggcaag atagtaggtg ccaagaacat ttaccttgaa
     9541 agcttccgtg ggattttttt ccattagcgg aacgtgtttg tgggccgcag catgaaaaac
     9601 tacatcaaag ctgaaacgtt caaatatctt tctgagcctt ttttcatccg ttatatcaca
     9661 gattatttca gtaattttca cctgcgggaa cttttcgttc agttcgtttt ttatgttaaa
     9721 tatactgttc tcgcctcttc ccattatata gagctcctta ggagcacata cggcagcctg
     9781 tctgcatatt tctgagccta tacttcctcc tgcgccggtt atcagaatat ttttcccacg
     9841 tatatactct gaaatctctt taagatcaac ttttacctct ttccttccaa gaaggtctgt
     9901 tacatcaact tctctcagta taccaagtga agccttactg ttaagtactt caaacattcc
     9961 gggcagaatc cttacccgta ctttttttgt atcaatgaag ccaagaagct ttctaaggtc
    10021 ggcagaagtt atatttgaaa ttgcaacaat tacctctttt attttgagca gatctatgta
    10081 tttcattgta ttttcaggta catcaaaaac tttatatccc cgtattgatc ttcctatttt
    10141 atcatagtca taatcaagaa aaccggcaac agttccttcg ccgggtctgc gttcaaactc
    10201 atcaaggatc tgaacaccta tctctcctgc tcctataatt agtatattgt tatctgtttc
    10261 tttaacagaa gatttgttag tctttgaaaa ccaataaatt ctgctccatg cgataagtat
    10321 gactccggta agcgatgtaa ttgcattgac ggtaaaagga agtataaaaa aagaagtaac
    10381 gtatctcctc agaaattcaa aaacaaaaag atttacaaga aagccaagtc cggctgaata
    10441 aaaaacaggt aaaagctgtg gtaaagctgc aaatctccaa atggactcat atatcctaga
    10501 tagataaaga gtaataacca ttatcaatgc atatagaaaa ataggcgaaa tatattttga
    10561 catctccgca aaatcaaact gaaaacgtat gaacatagat ataacatacg cacagacgaa
    10621 caatgcgtaa tcaattaaaa caagtgtaag cttcctttta gccatgtact tcattttgta
    10681 tctccaaagc agattcggta atacttttaa ccacatactc ctgctgttcc aaggttatat
    10741 ttgaaaaaaa tggaatagct accgtcaggc aggaagtaag ttcagtgtac ggaaaatcac
    10801 cctcttcata tccataaaga tctttgtaaa acttctgaag atgtactgga gaaaaataat
    10861 ttcttgcctg aacacctttt tttgaaagtt tttcgagcaa agaatcaaga attgtccgtg
    10921 cttttttaag ataagtatac cattcttcac ggtttttatg ttctataatg tcaggcatct
    10981 gataggtgct tatccagtta ggcaaaccca aaagttttga aatccatgct atatttattc
    11041 ttacaacgta aacaaaccag ctttccctag ttacatagtc aggaaccgta ggacaaacaa
    11101 aaaaactgtt tccttcaaaa agttttctat atctgtcagc tgctgatttg cgtttgtcaa
    11161 ggatctcatc cagtcttgtc atctgacagt atcctaaagc ggctgacatt tcatccatcc
    11221 tgtagttgta acccagccgg acatgctcga gccaggctcc gctttcaccg cgtccctggt
    11281 ttgacataga ctttacagcc ttgtaaatac cttcatcatc tgttactatt attccgcctt
    11341 ctcccgtagt aatctgtttg ttagggtaaa aggcaaaagc tcctgccaat ccgaaggtgc
    11401 cggctttttt gtttttatat tctgcgccca aagcttcaca ggaatcttca atcaccttaa
    11461 tctcactggg aattcttatt ttatcccagt caagcggcag cgaaaaaaca tctacagcca
    11521 ttaaaaaatc tgtttttata tgatttttag atggtctgta ggttccttca aaaaaacggt
    11581 tgagtttatc gattgatata ttgtaattat agggatctat atctataaaa acaggatcac
    11641 catgttcata aatagctacg tttgctgaag ctacaaatgt aaatggagta gtcagcattc
    11701 catgttcctt cgcaaaaccc attgatctga ggataacatg aagcgccgaa gtgccgctgt
    11761 tgactcccaa agcgtacttt aacccagtgt agctactaaa agaatcctca aactttttta
    11821 ggtaagggcc tagtgctaaa tcaggtgaat caagtacttg cataaccgta cttttttctt
    11881 tttcagttat atcaggatta gaaagcggta taagcatttt taccccctag atgaattctt
    11941 caattctgga atactctttt tgagttatct tattttcctg aagaagtctg tttagtataa
    12001 gcaaagcttt ttctttttcc atagctgtta ctttcctgaa gattatgcta aatctttctt
    12061 cgtcgcctat ggtgctaagt tctttgagca acaggtcata actgtcttct ggcggatcta
    12121 taatttgggg atcaatagtt tgattgcttt ttatggcctg ggtatacttg tcgctggcag
    12181 taccgtcttc aaagtaatga tatgcagttt ctcttaaaag aacataaaga agataccatt
    12241 catagaaatt aaaggttact attctgagta tgatggcaaa ggtgccgtca cggtagttta
    12301 tgatcctgtc ctttactatt ccgtatcgta tctctgattc ctgaagcatc cactgatcct
    12361 ttactatacg gtttatcatg agaacataag ataccataac cataaaacca cagccgatga
    12421 atgtataaac ctgccattga ggaagaacaa aactttctag ttcctgggta gaaagttcga
    12481 gaacctgtgg
//
LOCUS       DAOSHR010000043        12202 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_4389_length_12202_cov_5.503087, whole genome shotgun sequence.
ACCESSION   DAOSHR010000043 DAOSHR010000000
VERSION     DAOSHR010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 12202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12202
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4389_length_12202_cov_5.503087"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2315
                     /locus_tag="PLU28_03715"
     CDS             <1..2315
                     /locus_tag="PLU28_03715"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HOG34206.1"
                     /translation="GLEALIKELRIQKEEITSKIIAYKIAPKINAAGRMADAFTAFKL
                     LISQNHEEISKNVSSLIKLNTKRQAKEKEIYLYALSLIDVNPELKESKVIVLSGENWH
                     LGVLGIVASKLSALYNKPVLLVSKDEHSGKGSARSPAGINLMELFKEASKYNVFKEFG
                     GHELAAGFTIDSQDIDSLRISVNKAYEELYKEHVPYYEVFIDMEIENVWSSFFEDIER
                     LEPFGYRNPEPTFLIMNSHVDNFKFFGNGVENFAAKMRSQKDLIMDVIGYGLSQKMLD
                     LRYNSQLNLNMDMVGNFRVEKVHNSKISYLKYYLKDFEIKNFDVNSCEYKNVETNIIL
                     RDEIIRIGKLNVITDNLSSEKVSMFLPPRIKYATMLKKISDSLSASRKVVIIGSSHIV
                     LSHTYSIVSSYITPSNIYYNKKSRINSKVLSHESVFFVTLPAFLNNLSFFNGDNFDLI
                     VDEPYYMISHPAVQNMNDYNEFKKFISVKKSNIMVIGSLFTENIKDFFKHAGYKVLLA
                     NFNTFNYEVTKEEKPLLNILMDYNGSYGKKLIVINEKRKQEIISDLITEKFSIVEESI
                     KKFSGDMDFSKKLLLRESIKKDSVNYLITSYSNNGIAMDMKDSFPTFILLEVPKCRLE
                     LIDLVSSWNVGKKGSINLVLAYDDKFKTKMMYELQRMYPPASILKHTYDYIKNNPGIK
                     ESELITNLFKGDIEFCRTVFDELSDTGMVLNVSNKLEVFEEFDIKNIHNGLRVRESIL
                     DTYLIKNSIKTFESYDSRKIMDMFRVNLSE"
     gene            2321..2575
                     /locus_tag="PLU28_03720"
     CDS             2321..2575
                     /locus_tag="PLU28_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34207.1"
                     /translation="MLTTKDLNCIIRPEGTEPFEVFQILKRAGVFVKIFPAPKSIFMI
                     CAPVILISESSLEQVNMLLRCHGINYDNYILKKDIIQELL"
     gene            2577..3260
                     /gene="ruvX"
                     /locus_tag="PLU28_03725"
     CDS             2577..3260
                     /gene="ruvX"
                     /locus_tag="PLU28_03725"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOG34208.1"
                     /translation="MKVYGIDYGTVSTGIARGESILKIATGIGQVKTPEIFNFFLKEG
                     LSENNVVALGLPVSMSSRFSEQTFKTVDFALKLKEKFGAKIYLIDERLTTSMVYSTMK
                     NARSTKGYKDSKDQNSSVLILSSFFSNEKGYSELIPGKVFDIGIVEKDNLMVYNTAVS
                     VSSLKGKAVDIFTTDPWIFWYYFKNGFKSTTIEADLKVSYESIYTFMVEEKYLKEKFL
                     CECVCSLDG"
     gene            3238..3313
                     /locus_tag="PLU28_03730"
     tRNA            3238..3313
                     /locus_tag="PLU28_03730"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3272..3274,aa:Arg,seq:ccg)
     gene            3345..4349
                     /locus_tag="PLU28_03735"
     CDS             3345..4349
                     /locus_tag="PLU28_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOG34209.1"
                     /translation="MAKHDLGIDLGTATFVVYKRGEGIIIQEPSMVAINKRKGQILAI
                     GAAAKEMIGKTPEEISIVKPIQDGVISDPDVIEEVLRYFIKKAKETFALSKPNLVIGI
                     PALTTDVERRAVVDAAERVGAARADLVIEPLAAAIGSGIDIVKPDGNMIIDMGGGTTD
                     IVVISLGGIVVSDSIKLAGQAMDAEIIKYVKRVYRFLIGESTAEQIKIKIGKAYEKEE
                     TIELEVKGQDLKTGLPSSIKLNSEDVFKAIKEILDELISHIRMVLEKTPPELASDILK
                     NGIILTGGTALTRGIDKFIEEKTCVKTRITDEPHLTVAKGTGILLDDIELLSRVSVDY
                     "
     gene            4389..5108
                     /locus_tag="PLU28_03740"
     CDS             4389..5108
                     /locus_tag="PLU28_03740"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body protein"
                     /protein_id="HOG34210.1"
                     /translation="MDRGIYYATMGMLNNLYQLDNIANNLANVNTNGYKKDSVSFKAV
                     MEKEFYSYDGKTQKIKKIGNMETATVVDDVRPILFSGGFEETDNQYDFAIEGSGFFKI
                     QRGEEFYYTRNGEFKLDSQGFLVTNDGDYVLNRNNGKILINNDFQVLPDGKVSGTDYE
                     IAVFDLNSTKKYGYNLFTGEEVVTSDFKVMQKTLEHSNVDALNEMIKMISASRQFGIL
                     EKAVQTSDSLNARIIETVSKI"
     gene            5152..5937
                     /gene="flgG"
                     /locus_tag="PLU28_03745"
     CDS             5152..5937
                     /gene="flgG"
                     /locus_tag="PLU28_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HOG34211.1"
                     /translation="MLISLYSAATGMNAEQSRLDIIANNLSNVNTIGYKTVRAEFQDL
                     LYQSMKEAGSPTAQNSVLPTGLYVGHGTRFSSTNRIFTVGNIEETNHSLDVAIMGDGF
                     FQIQLQDGRIGYTRDGAFKMDATGRIVTSNGLVLVPNIVVPENAMAISISPDGIVSAQ
                     LPDDTVENLGNITTVRFINPSGLKSIGSNLFLQSASSGAPIEGIPNQDGFGALQQSSI
                     EKSNVDVVKEMVNMIVAQRSYDINSKTINSADEMLRTVAGLKR"
     gene            5939..6868
                     /gene="flgA"
                     /locus_tag="PLU28_03750"
     CDS             5939..6868
                     /gene="flgA"
                     /locus_tag="PLU28_03750"
                     /inference="COORDINATES: protein motif:HMM:TIGR03170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HOG34212.1"
                     /translation="MKSKTILMLFCFFGFTVFSAVEITVPGTIVSDDQYFTVKDIWPE
                     FPYDRTIAFIGKSGLTYTSEELAKTLYGLMKPYDIKYELEFVSKDIKILYSQYMFKEE
                     QSGIDIKKDILEKMQKNYPEYVFSEHVITRPAVEKVISYEINSYGLLKDLLNVTFTAK
                     MPDGKKTIGTLSVTVEKNVKVLVVLQDIKKNQQIKADLTCESTVNVLKYEFNMITKED
                     LENFAYTTLRDLKAGEPINNAFVKKIPDLKAGQTVEIVVDYGTVMVRATGRLMKDASY
                     GEVVQIRNTDTGVLLEGLLAPGAKVLINMNGGD"
     gene            6872..7483
                     /locus_tag="PLU28_03755"
     CDS             6872..7483
                     /locus_tag="PLU28_03755"
                     /inference="COORDINATES: protein motif:HMM:NF014195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HOG34213.1"
                     /translation="MKNKILLFLAVILSFYAFGQSLWNGSSGKSAFAPNNKTYKAGDI
                     ITIEVLESPSLSVSDNMADYNSAARNTVGSVFKTIGGVDLKSFVPLTTTDPSAVKLGN
                     KQTQSSSQASIKLYIAAQIIEENNGLLKIKGEKEIKVGKERKKVIVEGLLAASSISSS
                     GTVESSQLAEARIWYDGDVVFQQDPNEKSWLSWVLSGISNMFF"
     gene            7534..8520
                     /locus_tag="PLU28_03760"
     CDS             7534..8520
                     /locus_tag="PLU28_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HOG34214.1"
                     /translation="MKRQIFLVLVFAFLITASAGVRIKEIANFRGARDNQLFGLGLVT
                     GLNGTGDSGLVTSELMTNIFKNMGYSVQSQFKTKNTAVVMVFADIPPFYKEGMKLDIT
                     VASIGDAKSLENGFLVQTPLLGADNKVYAVAQGAVFTGGADAKGSSNTQKKYRITGSV
                     PQGAIVEAEIPVNIFSDDMVSINLDSPDITTAARVAMAINVSFSEKIAKAQDPSTIRV
                     DIPEIFKDDVISFLSLIEEVEVEKDQKAKIVLDEKTGTIILGGKVKVGDFSLSYGSFV
                     LSVVNGQVGEKEATVDNIISALKAAGAVPQDILVIIKNASKAGYVMAELVVI"
     gene            8521..8796
                     /locus_tag="PLU28_03765"
     CDS             8521..8796
                     /locus_tag="PLU28_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34215.1"
                     /translation="MAGLGPVYLTAVSSKNLKNEDVAIEFVSGIFSDILNKMQESDLF
                     KNELFPESSAQSWYKEMINSEYSRTIVKQSFQPLIKELTEKFNKPSR"
     gene            9019..10350
                     /gene="gcvT"
                     /locus_tag="PLU28_03770"
     CDS             9019..10350
                     /gene="gcvT"
                     /locus_tag="PLU28_03770"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HOG34216.1"
                     /translation="MFVQVFKFLRFPEYDIYKQILEQNEITVMLKSSKNMLPSSIIGE
                     PAMYDIYVQQDDKEKALIIISDFLKEDIQMSQLKRTPLYEQHEKLGAKLVEFGGWEMP
                     VWYTSIIEEHKTVRETVGLFDVSHMGEIEVKGKDAQKFVEYIITNNVTKLPYGGIVYS
                     PMCNEKGGVVDDLLAYKINEEYYLLVVNASNIDKDYSWIMSHTKGFSVQVKNLSQEYG
                     QIAVQGPKAEEVLQKITTGDLKNIAFYNFIQTEVMGFKAIVSRTGYTGEDGFEVYLDA
                     DNTVALWEKLLELVKPYNGIPCGLGARDTLRFEAVYMLYGNELNDVGTPLEAGLKWAV
                     DLEKDFIGSDILKKQEETGLTKKLRGIEILDKVPARHGYIVMADGKEIGVITSASKSI
                     STGKNLALGYIDAQYCKFGTDVQIQIRDKSVPAKIIKTPFYRGSVKSSKTK"
     gene            10371..11714
                     /gene="gcvPA"
                     /locus_tag="PLU28_03775"
     CDS             10371..11714
                     /gene="gcvPA"
                     /locus_tag="PLU28_03775"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HOG34217.1"
                     /translation="MDYPFLPHTEEEIGQMLESIGLKQIDDLYSDVPVLLKEELSIAP
                     SKSEMQVREIMKELSCRNISLDDYGIFRGAGVYNHYIPSAVYQLASKRGFLTAYTPYQ
                     AEVSQGTLQMLFEYQTMICEITGMEVANSSMYDGASAMAESVLMAQRVTKKDKVLICD
                     TVHPEYIQVTRTYTEPVNTVLELLKHDSKTGQADLQDLKAKIDDSTAAVVVQYTNFYG
                     VIEDIKTLRENIPSHILLIVVANPISLGMLQAPGKLGADIVVGEGQCLGNNCNFGGPG
                     FGFFASKHSYIRQMPGRIIGETLDREGKRGFVMVLQTREQHIRREKATSNICSNQAHN
                     TLIASIYLSFLGKSGLKEVAYQCYHKAHYLAEKLMESEKFEPVFSGPFFNEFVLKSSV
                     PVGIMNQKLLEQKYIGPLNLEKVTGRDKDCALFCATEMTKKEEIDYITSFLEAMI"
     gene            11711..>12202
                     /locus_tag="PLU28_03780"
     CDS             11711..>12202
                     /locus_tag="PLU28_03780"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295668.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 1 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HOG34218.1"
                     /translation="MKFIFEHSVEGKKAFRLEELDVPLSQIDFPQYLLRDVPPELPQV
                     SELDIVRHYTQLEKLNHSVDSGFYPLGSCTMKYNPFINDETASYAGFSKIHPYEEEDS
                     VQGALELMYNLQQSLCEITGMDAITLQPAAGAHGELTGMLIIRKYLLDKGFTNKTKVI
                     VPDS"
BASE COUNT         4144 a         1869 c         2562 g         3627 t
ORIGIN      
        1 ctggattaga agctttaatt aaagaacttc gaattcaaaa agaggaaatc acttccaaga
       61 taattgccta taaaattgcc cccaaaataa acgctgcagg cagaatggct gatgccttca
      121 ctgcttttaa gcttctcata tcccaaaatc atgaggaaat tagtaaaaat gtctcatctc
      181 taataaagct taacactaaa agacaggcta aagaaaagga gatatatcta tacgctctga
      241 gtctgataga tgtaaacccc gaattgaaag agagcaaagt gatagtgttg tcgggtgaaa
      301 actggcatct tggagtgtta ggcatagtcg cctcaaagct ttcggccctt tacaataagc
      361 cggtgctttt ggtttcaaaa gatgagcatt ccggaaaagg atcggcaaga agcccagctg
      421 gaataaatct tatggagctg tttaaagaag cctctaagta caatgttttc aaagaattcg
      481 gaggacatga gctggccgca ggctttacaa ttgattctca ggacatagac tcacttagga
      541 tatctgtcaa taaggcgtat gaggagttat acaaggaaca tgtaccgtat tacgaagtat
      601 ttatagatat ggaaatagaa aatgtatgga gcagtttttt tgaagatatc gaaagacttg
      661 agccttttgg ctacagaaat cccgagccga cgtttcttat aatgaactcc cacgtagata
      721 attttaagtt ttttggtaac ggagtagaga attttgcagc gaaaatgcgc agtcagaaag
      781 atctcatcat ggatgttata ggttacggtc tttctcagaa gatgctcgat ctgagatata
      841 acagccagct taatcttaat atggatatgg ttggcaattt cagggtagag aaggtacata
      901 attcaaaaat aagctatctt aaatattatc tgaaagactt tgaaattaag aattttgatg
      961 ttaattcctg tgagtataag aatgttgaaa ccaacatcat actgcgagat gagattatcc
     1021 gtataggcaa attgaatgtt attaccgaca atctctcttc agaaaaggta agtatgtttc
     1081 tgccaccaag gatcaaatat gcaacaatgc tgaagaagat ttcagattct ctttctgcaa
     1141 gcagaaaagt tgttataata ggatcgtcgc atatagttct gtcgcataca tatagtatag
     1201 tttcatcata tattactcca tcaaatatat attacaataa aaaatcacga ataaacagca
     1261 aagttctttc acatgaaagt gtttttttcg ttacgcttcc tgctttttta aacaatctgt
     1321 cattttttaa cggagataac tttgacctta ttgtggacga accatattac atgatatcgc
     1381 atcctgcagt tcagaacatg aatgattata atgagtttaa aaagtttatt tcagtaaaaa
     1441 aaagtaatat aatggtcata ggctctcttt ttactgaaaa tatcaaggat ttttttaaac
     1501 acgcaggata caaagtcctt ctggcaaact ttaatacctt taactatgaa gttaccaaag
     1561 aggaaaaacc tcttttaaat attcttatgg actataatgg aagttacggt aaaaaactta
     1621 tagtcataaa tgaaaaaagg aaacaggaaa ttatttcaga tttaataact gaaaagttta
     1681 gtattgtaga agaatcaata aaaaagttca gcggtgatat ggatttttca aaaaaattac
     1741 ttctaaggga aagtataaaa aaagattctg tgaactatct gattacttcg tattccaata
     1801 acggtattgc gatggatatg aaagattcgt ttcctacctt catcctgctt gaggtaccaa
     1861 aatgccggct ggagcttata gatttggtgt cttcatggaa tgttgggaaa aaaggaagca
     1921 taaatcttgt actggcatat gatgacaagt ttaaaaccaa gatgatgtat gagcttcagc
     1981 gcatgtatcc tcctgccagt attttaaaac atacatatga ctatataaaa aataatccag
     2041 gaataaaaga gagcgaactt ataaccaact tgttcaaagg agatatagaa ttttgccgga
     2101 cggtgtttga tgaactttct gatacaggca tggttcttaa tgtatcaaac aaacttgaag
     2161 tttttgaaga atttgatatt aagaatattc ataatggtct gagagtcaga gaaagtattt
     2221 tagatacata ccttataaaa aactcaataa agacttttga aagttatgat tccagaaaaa
     2281 taatggatat gttcagagtt aatctttcgg agtgatataa atgctgacta caaaagattt
     2341 aaactgtatc atcagacctg aaggaacaga accttttgag gttttccaga tactaaaaag
     2401 agcaggtgta tttgtgaaaa tatttcctgc tcccaaaagc atttttatga tctgtgctcc
     2461 ggttattctg atttccgaaa gttctcttga gcaagttaat atgcttttaa gatgtcatgg
     2521 tattaattac gataattata ttctaaaaaa agatataatt caggagctgc tttgatatga
     2581 aggtctatgg aatagattat ggaactgtaa gtacaggaat tgctcgaggt gaaagtatcc
     2641 ttaagattgc cacaggaata ggccaggtaa agacgccgga aatcttcaac ttttttttga
     2701 aggagggtct ttctgaaaat aatgttgttg ctttagggtt gccggtttcc atgtcgtcaa
     2761 gattttccga gcagacgttt aaaacagttg attttgcatt aaaactaaaa gaaaagtttg
     2821 gtgccaagat ttatcttatc gatgaaaggc ttaccacaag tatggtatat tcaaccatga
     2881 aaaatgcccg ttcaaccaaa ggatataaag attctaaaga tcaaaatagc agtgtcctta
     2941 tactaagctc ttttttttct aatgagaaag gttattctga gcttataccc ggaaaagttt
     3001 tcgatatagg tatcgttgaa aaagataact taatggttta taatactgcg gtaagtgttt
     3061 cttcactcaa aggaaaggca gtagatattt ttactacaga tccatggatt ttttggtatt
     3121 attttaaaaa cgggtttaaa agcaccacga tagaagcgga tctgaaagtt tcatatgaaa
     3181 gtatatacac gtttatggta gaagaaaagt atcttaaaga aaaattttta tgtgaatgtg
     3241 tctgtagctt agatggatag agcaccggac tccggatccg ggaatgcggg ttcaagtccc
     3301 gccagacacg ccatagtata atgcaaaatg caggaggtat agatatggca aagcatgatc
     3361 tgggaattga tcttggaacg gcaacttttg ttgtatataa gcgtggcgaa ggaataatta
     3421 tacaagaacc gtcgatggtt gcaataaata aacgcaaggg tcagattctt gccattggtg
     3481 ctgcagcaaa agagatgata gggaaaaccc cagaagaaat ctctattgta aagcctattc
     3541 aggacggagt tataagcgat ccggacgtaa tagaagaggt gcttaggtac ttcataaaaa
     3601 aggcaaaaga gacttttgct ctttcaaaac ctaatttagt aattggcata ccggccctaa
     3661 caaccgatgt tgagagaagg gctgttgttg atgcagcaga aagggtggga gctgcaagag
     3721 cagatctggt aatagaaccg ttggcagctg ccattggttc aggtatagat attgtaaagc
     3781 ccgacggaaa tatgattatt gatatgggtg gtggaacgac ggatattgtt gtcataagtt
     3841 taggtggaat tgttgtaagt gattccataa agctggcagg ccaggcaatg gatgcagaaa
     3901 taataaagta tgtaaaaaga gtttacaggt ttctgatagg tgaatcaacg gctgaacaga
     3961 taaagatcaa aatcggaaaa gcgtatgaaa aagaagaaac tatagagttg gaagtaaagg
     4021 gccaggattt gaaaacaggt ctcccctctt caataaaatt aaattctgaa gatgttttta
     4081 aagctataaa agaaattttg gacgagctta tatcgcatat aagaatggtt ctagaaaaaa
     4141 cacctccaga gcttgcctcg gatattctta aaaacggcat catattaacc ggaggtactg
     4201 cgcttaccag aggaatagat aagtttattg aagagaaaac atgtgtaaaa acaaggatta
     4261 cagatgaacc tcatcttact gtcgcaaaag gtacaggaat tttgttggat gatatagaac
     4321 tattaagtag agtcagcgtt gattattgaa acacgttgat aatttacttg atagcagagg
     4381 tggaattaat ggaccgtggg atttattatg caactatggg gatgctgaac aatctttatc
     4441 agttggacaa catagccaat aatctcgcga acgtcaatac caacggatat aagaaggatt
     4501 ctgtttcttt caaagccgtt atggaaaaag agttttactc atatgatggt aaaactcaaa
     4561 aaataaaaaa gattggtaat atggaaactg ccacagttgt tgatgatgta agacctattt
     4621 tatttagcgg tggtttcgaa gaaacggaca accaatatga ttttgcgata gaaggttcag
     4681 ggttcttcaa aattcagcgt ggagaagaat tttattatac acgtaacggg gagtttaaac
     4741 ttgattctca aggattttta gtcacaaatg acggtgatta tgtgttaaat cgtaataacg
     4801 gaaaaattct tataaataat gactttcagg ttttaccaga tggtaaggta tcaggaaccg
     4861 attatgagat agctgttttt gatcttaatt ccactaagaa gtatggatac aatcttttta
     4921 ccggagagga agttgtgact tctgatttta aagtgatgca aaaaacattg gagcattcaa
     4981 atgttgatgc actgaatgag atgataaaga tgatttctgc ttccagacag tttggcatcc
     5041 ttgaaaaggc agttcagaca agcgattctc taaatgcaag gataattgaa actgtaagca
     5101 aaatataaac ttatttaaaa taatcagata aaaagaacgg gaggtagttt tatgcttatt
     5161 tctttataca gtgcagctac aggtatgaat gcagaacagt caagacttga tataatagca
     5221 aataaccttt caaacgtcaa cacaatcggt tacaaaacgg ttcgtgcaga gtttcaagat
     5281 cttttatatc aatccatgaa ggaagctggc tctccgactg cccaaaactc agttctccca
     5341 acggggcttt atgttggaca cggaacaaga ttttcttcta caaacaggat attcacagtt
     5401 ggaaatattg aagaaaccaa tcattctctt gatgtggcaa taatgggtga tggttttttt
     5461 cagatacagc ttcaggacgg aagaatagga tatacaagag atggtgcttt caagatggat
     5521 gcgaccggga ggatagtaac aagcaacggt cttgttttgg ttcccaatat agttgttcct
     5581 gaaaatgcta tggctataag tatttctcct gatgggattg ttagcgctca gcttcctgac
     5641 gatacagttg aaaatcttgg caatataact acagtacgct ttataaatcc ttcaggttta
     5701 aagagcatcg gttccaattt gttccttcag tcggcttcat ccggagcacc tattgaagga
     5761 atacccaatc aggatggttt tggagctttg cagcagtcct caatagaaaa atctaatgta
     5821 gatgtggtaa aagaaatggt aaatatgata gttgctcaac gttcgtatga tataaactct
     5881 aagactataa actctgcgga tgaaatgctt aggacagttg ccggtttgaa gagataatat
     5941 gaaaagtaaa acgatactta tgcttttttg tttctttgga tttacggttt tttctgctgt
     6001 ggagataacc gtacctggaa caatagtatc ggatgatcag tactttacag taaaagatat
     6061 ttggcctgag tttccatatg acagaactat tgcatttata ggaaaatcag gccttacata
     6121 tacttccgaa gagcttgcaa agacgttgta cggacttatg aaaccgtatg atataaagta
     6181 tgagttggaa tttgtttcga aagatataaa aattttatac agtcagtata tgtttaagga
     6241 agaacaatct ggtattgata taaaaaaaga cattcttgaa aaaatgcaga aaaactatcc
     6301 ggagtatgtt ttttcggaac acgttattac aagaccggca gtagaaaaag ttatatcata
     6361 tgagatcaat agttatggat tgttaaaaga tcttctgaat gttactttta cagcaaaaat
     6421 gcctgatgga aagaaaacaa taggaacttt atccgtaacg gttgaaaaaa atgtaaaggt
     6481 tcttgtagtt ttacaggata ttaaaaaaaa tcaacagata aaagccgatc ttacttgtga
     6541 atcaaccgta aatgtcttga agtatgagtt taatatgata accaaagagg atttggagaa
     6601 ctttgcttat acaacgctca gagacctgaa agccggggaa cccataaaca atgcttttgt
     6661 aaagaaaata cctgatctta aggcaggcca aaccgtggaa atagttgttg attacggaac
     6721 ggtaatggtc agagcaacag gtcgtctgat gaaggatgct tcatacggtg aggttgttca
     6781 gatacgtaac actgatacgg gagttctttt agagggattg cttgctccag gggcaaaagt
     6841 tttaattaat atgaacggag gcgattgaaa gttgaaaaat aaaatcctgc tttttttagc
     6901 ggtaatttta tctttttatg cttttggaca gtctttatgg aacggttctt caggtaaaag
     6961 tgcttttgcg cctaacaata aaacatataa ggcaggagac ataataacaa tagaagttct
     7021 tgaaagtccg tcactttcag taagtgataa tatggcagat tataattctg ctgcaagaaa
     7081 tactgtagga tcggtattta aaaccattgg aggagttgat ctaaaaagtt tcgttcctct
     7141 tacaactact gatccctcgg cagtaaagct tggcaataaa caaactcaat cttcttctca
     7201 ggcttctata aaactttata ttgcagctca gattatcgaa gaaaacaacg gattgcttaa
     7261 aataaaaggt gaaaaagaaa taaaagttgg aaaagagagg aaaaaggtta ttgttgaagg
     7321 actactagca gcttcaagta taagctccag tggtacggtt gaatcatccc aactggctga
     7381 agcaaggata tggtatgacg gtgatgtagt atttcaacaa gatccaaatg aaaaatcatg
     7441 gctaagttgg gtactttcag gtatttcaaa tatgtttttc tgaggtataa ctataaaatt
     7501 atctttattt aagtaatttc tgagaggtgt tttatgaaaa gacagatttt tttagttttg
     7561 gtttttgctt ttttgataac ggcttctgcc ggagtcagaa taaaagagat agctaatttt
     7621 agaggtgcaa gagataacca gctttttggt ttgggacttg ttacagggct taacggtact
     7681 ggagactcag gacttgtgac ctccgagctt atgaccaata ttttcaagaa catgggctat
     7741 tcagtacagt cacagttcaa aactaaaaac actgctgtgg taatggtttt tgcggatatt
     7801 cctccttttt acaaagaagg catgaagctt gatataacgg ttgcttcaat tggagatgcc
     7861 aaatctttgg aaaatgggtt tcttgttcaa acgcctcttc ttggtgcaga caataaagtt
     7921 tacgcagttg ctcaaggagc tgtttttaca ggtggagctg atgcaaaggg aagttcgaat
     7981 acacaaaaaa aatacagaat aacaggctct gtgcctcaag gagctatagt agaagcagaa
     8041 atacctgtga atattttttc ggatgatatg gtttcgataa atcttgactc tcccgatata
     8101 acaactgcag cgcgtgtggc aatggctata aatgtatctt tttcagagaa aatagcaaag
     8161 gcgcaggatc catccacaat aagagttgat attcccgaaa tttttaagga tgatgttatt
     8221 tcatttttat ccctcataga ggaagttgaa gttgaaaagg atcagaaagc taagattgtt
     8281 cttgatgaaa aaacaggtac cattattctt ggaggaaaag tcaaggttgg agacttttct
     8341 ctaagttatg ggagttttgt actttctgta gtaaatggtc aggttggtga aaaggaagcc
     8401 actgtggata atattatctc ggcgcttaaa gcggccggag ccgttccgca ggacatactt
     8461 gtgattataa aaaatgcttc aaaagccggc tatgtaatgg cagagctggt tgtgatataa
     8521 atggctggtc tgggtcctgt ttatctcact gctgtaagtt ctaagaacct taaaaacgaa
     8581 gatgttgcaa tagagtttgt atcaggtatt ttttcagata tccttaataa gatgcaggaa
     8641 tctgatcttt ttaaaaatga gttgtttcca gagagttcag cccagagctg gtataaggaa
     8701 atgattaatt cagagtattc gagaacaatt gtaaaacaaa gttttcagcc tttaataaaa
     8761 gaactgaccg aaaaatttaa caagccttcg cgataatgcc aaacattagt ttggcattat
     8821 tattatacag gcagtattaa aaaaataagt ataataaagc tattttattt tattaaattc
     8881 caaaggctat tatataacta tatccgagta tttggcgtga aaaaaatttc attattaatg
     8941 atataattaa atgggtagat attattacgc atttctttta aattgattct agtatagaag
     9001 ctataaaggg gtgatactgt gtttgttcag gtgttcaaat ttttaagatt tcctgaatac
     9061 gatatctata aacagatact cgagcaaaat gagatcactg ttatgctgaa aagttccaag
     9121 aacatgttgc cctcttctat aatcggtgag ccggcaatgt acgatatata tgttcagcag
     9181 gacgataaag aaaaggcttt aattatcatt tctgattttt taaaggagga tattcaaatg
     9241 tcacaactca aaagaacgcc gctttatgag cagcatgaaa agcttggagc aaagttagtg
     9301 gagtttggcg gatgggagat gcccgtatgg tatacttcaa taatcgaaga acacaaaaca
     9361 gtaagagaaa ctgtcggtct tttcgatgtt tcccatatgg gagaaattga agtcaaaggt
     9421 aaggatgccc agaaatttgt agagtatatt atcacaaata acgtaactaa actgccctac
     9481 ggaggaatag tttacagtcc aatgtgtaac gaaaaaggcg gagtggttga tgatctttta
     9541 gcctacaaaa taaacgagga atactatctt ttagtggtta atgcttctaa tatcgataaa
     9601 gattattcat ggattatgag tcatacgaag gggttttctg tccaagtaaa aaatctttca
     9661 caggaatatg gacagatagc agttcaaggt ccaaaagcgg aagaagttct tcagaaaatt
     9721 acaacaggcg atctcaagaa tatagctttt tataatttta tacagacaga agtaatgggt
     9781 tttaaagcaa tagtatcacg aacgggctat accggagaag atggttttga agtttatctt
     9841 gatgctgaca ataccgttgc cttgtgggaa aaacttcttg aattagttaa gccatataat
     9901 ggaataccct gtggtttagg agcaagagat acactacgtt ttgaagctgt atatatgctt
     9961 tacggaaacg aactaaatga tgttggaact cctcttgaag ctggtttaaa atgggctgtc
    10021 gatcttgaaa aagactttat aggaagcgat atacttaaga agcaggaaga gacaggactt
    10081 actaaaaaac tcagaggaat tgagatactt gataaagttc cggcaagaca tggctacata
    10141 gtaatggctg atggcaagga gataggagta ataacaagtg cttcaaaatc aatttctaca
    10201 gggaaaaatc ttgcacttgg ctatatagac gcacagtact gtaaatttgg taccgatgtt
    10261 cagatccaga ttagagacaa gtcagttcca gcaaagataa taaagactcc tttttataga
    10321 ggcagtgtaa aaagttcaaa gacaaaataa ttttatagag gtgagttcaa atggattatc
    10381 cttttctgcc gcatacagag gaagaaatcg gacagatgct tgaaagcata ggtttaaaac
    10441 agatagatga tctttattca gatgttcccg tcctattaaa agaagaatta agtatagctc
    10501 cttcaaaatc agagatgcag gtacgtgaaa taatgaagga gctgtcatgc agaaatataa
    10561 gcttagatga ttacggtata tttaggggag caggggtata taaccattat attccttctg
    10621 cagtatatca gcttgcatct aaaagaggat ttttgactgc ctacacacca tatcaggcag
    10681 aagtatctca gggaaccctt cagatgcttt ttgaatatca gactatgata tgtgagatta
    10741 cgggtatgga agttgctaac tcctctatgt atgacggagc aagtgcaatg gctgaaagcg
    10801 ttcttatggc tcagagagta accaagaagg acaaagttct tatatgtgac acagttcatc
    10861 ctgaatatat tcaggtaaca aggacatata cagagcctgt caatactgtt ctggagcttt
    10921 taaaacacga ttctaaaacc ggtcaggctg atcttcagga tctaaaggcc aaaattgatg
    10981 atagcacagc tgctgtagtg gttcagtata ctaattttta cggagtaatc gaagatataa
    11041 agactttacg tgagaatatt ccgtcgcata ttcttttgat agttgttgcc aatcctatat
    11101 ctttaggaat gcttcaggca ccaggcaagc ttggagcaga cattgttgtt ggtgaaggac
    11161 agtgccttgg aaataattgt aacttcggag gtccgggatt tggttttttt gcaagtaagc
    11221 attcttatat aagacagatg ccaggtagaa taataggaga gactcttgac cgtgaaggta
    11281 aaaggggatt tgttatggtt cttcagacaa gagagcagca tataagaaga gaaaaggcta
    11341 cttccaatat ctgttctaac caggcacata atacccttat tgcttcaata tacctttcat
    11401 tcttgggaaa aagcggattg aaagaagttg cttatcagtg ctatcataaa gcacattatc
    11461 ttgcagaaaa gcttatggaa agcgaaaaat ttgaacctgt tttctctggt cctttcttca
    11521 acgagtttgt ccttaaatcc tctgttccgg ttggaataat gaatcaaaag cttcttgaac
    11581 agaaatatat aggtcctctc aaccttgaaa aggttaccgg aagagacaaa gattgtgccc
    11641 tcttctgtgc aacagagatg acaaagaaag aagaaattga ctacataaca tcttttctgg
    11701 aggcgatgat atgaaattta tatttgaaca ttctgtagag ggtaaaaaag ctttcagact
    11761 tgaagaactt gatgttcctt tatcccagat tgactttcca cagtaccttc tgagagacgt
    11821 accacctgaa cttcctcagg tgagtgagct tgatatagta cgtcattata ctcagcttga
    11881 aaagcttaat cattctgttg acagcggctt ttatccttta ggttcttgta caatgaagta
    11941 caatcctttc ataaatgatg aaaccgcttc ttatgcagga ttttctaaaa tccatcctta
    12001 tgaggaagaa gatagtgttc aaggtgctct tgaacttatg tataatcttc agcagagctt
    12061 gtgcgaaata acaggaatgg atgccattac tcttcagccc gcagcaggtg cccacgggga
    12121 actcacagga atgcttataa ttcgaaaata tttgctggat aagggtttca caaacaagac
    12181 caaggtaatt gtaccggatt cg
//
LOCUS       DAOSHR010000044        12007 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_4491_length_12007_cov_4.641566, whole genome shotgun sequence.
ACCESSION   DAOSHR010000044 DAOSHR010000000
VERSION     DAOSHR010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 12007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12007
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4491_length_12007_cov_4.641566"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1390
                     /gene="purH"
                     /locus_tag="PLU28_03785"
     CDS             <1..1390
                     /gene="purH"
                     /locus_tag="PLU28_03785"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HOG34219.1"
                     /translation="YLKDNGIQVKSISSFTGFEEILDGRVKTLHPKVYGGILAIRDKQ
                     EHMQELQSMDIKTIDMVVVNLYPFFEKAVQDISFEDKIEFIDIGGPSMLRSAAKNFKD
                     VIVLSDTTDYSVVIEQLKNNGDVEQKIRRELAAKAFNLTSAYDAAISSFLTGKEFPEY
                     LSLSYTLKQKLRYGENPHQKSVLYARTDKKGLINNCEQISGKELSYNNIRDMDVAYKL
                     VTEFDEPACCAVKHNSPCGAAVAQNIYDAYTQVYECDPVSIFGGIVAFNRPVDLKTAE
                     KLHEIFLEIIIAPDFEPEALLLLKSKKNLRIIKCDCTISDKLEMITVDGGVLVQSIDR
                     DFKEEYKVVTKTSPTQEQLRQMKFGMIVAKYTKSNAIVTVKQMHTASIAGGQVNRIWP
                     AVQAMQRGKGAQLLCSDAFFPFDDIVQAAYEYGIKAIMQPGGSVNDKASIELCDKYGI
                     SMVFTGIRHFKH"
     gene            1406..2650
                     /gene="purD"
                     /locus_tag="PLU28_03790"
     CDS             1406..2650
                     /gene="purD"
                     /locus_tag="PLU28_03790"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOG34220.1"
                     /translation="MKVMIIGSGGREHALAHKLSQSPDIDCIYCVPGNGGTALEDKCF
                     NVELNNIEEISAFALGKNIDYTFVGPEAYLVQGITDIFSQRKLKIIGPDKKASMLESS
                     KSYAKEFMKKYNVRTAAYDTFSEYEKAIDYILKADFPLVIKADGLAGGKGVVIAQNYE
                     QAVETLNFFMKEDIFKGSGKTVVIEEFLKGFETSVLAAVDGKTILPFISAKDHKQAYD
                     GNKGPNTGGMGAIAPNPLFTEEHFEDFKNNIMLPTLRGIREEKLNYKGIIFFGLMICT
                     KGVYLLEYNVRMGDPETQAVLPLMETDFFQVIKHIYDGSLDGYEIKWKKGSCCCVVAA
                     SHGYPGKYETGFEITGYDQVASKVYSAGTVFKNGKLISSGGRVLNISSLGDNLFQARQ
                     KAYMDLAKIKFENIYYRNDIGG"
     gene            2652..3941
                     /gene="purB"
                     /locus_tag="PLU28_03795"
     CDS             2652..3941
                     /gene="purB"
                     /locus_tag="PLU28_03795"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOG34221.1"
                     /translation="MIERYAMQPLKDLWNEENQYKRWLEVEVSVVKAFEKYGQAPVGT
                     SQQILSKAVIDVQKIHEIEAVVDHDVIAFINCVTQKMGDEARYFHKGMTSSDVVDTAL
                     SLALKRSGLVIKQELETFEEVLKEQAFKYKDTITIGRTHGIHAEPTSFGLKLLSYLAE
                     VQRNKVRLDAAIENISYAKISGAVGNYANIDPEIESLALQMIGLKPCKVSTQIVPRDI
                     HAEFLNTLALIGANIERLATEIRHLQKTEILEVQEPFKKGQRGSSAMPHKKNPILCER
                     LCGMSRMLRSYTIAGYEDTVLWHERDISHSSVERMVFPDATMITYYMINKSVWLVKNL
                     VVYENKMKENFKASHNLVFSQRLMLAMIDKGFSREESYKLIQELSMRSWNESIDLMQV
                     VLNDERTSSKFNENEIKEIFDPAYYLRNIEEVFKRFI"
     gene            4253..4516
                     /locus_tag="PLU28_03800"
     CDS             4253..4516
                     /locus_tag="PLU28_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34222.1"
                     /translation="MAIAVTCADNPMNEIRSIGFIIYFNKDIYCWLSLHTWNRGASNM
                     AYTEYILLGNMFLKIFFFFFKKLFPFRIIIRNYYWEKLSEFLC"
     gene            4816..4953
                     /locus_tag="PLU28_03805"
     CDS             4816..4953
                     /locus_tag="PLU28_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34223.1"
                     /translation="MKKKTINFKEKDKNYGNSQVKESNRITPKKTTRNLGAGLRQSKK
                     G"
     gene            5155..5832
                     /locus_tag="PLU28_03810"
     CDS             5155..5832
                     /locus_tag="PLU28_03810"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOG34224.1"
                     /translation="MEDMNITRMPLIGDKAPEFKAKTTQGDINFPQDYKGKWVILFSH
                     PADFTPVCTTEFMTFATMADEFRALNTELVGLSVDSLYAHIAWLRKIQEIEWNGMKHV
                     EVKFPLIEDIKMDVAHKYGMIQPGQSSTQAVRAVFIIDPNAVIRTILYYPLSTGRNFD
                     EIKRIILALQKADNDKVATPANWKPGDDVIVPTAGSCGVAKERMESKSEDTYCLDWFM
                     CFKKEKK"
     gene            complement(5898..6218)
                     /locus_tag="PLU28_03815"
     CDS             complement(5898..6218)
                     /locus_tag="PLU28_03815"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOG34225.1"
                     /translation="MKIGLCAKHETKSSQVDDRFARASFFVVYNSSTSEYTFFKNPFD
                     VEHGAGPKVIEFFAEKGVTVLIAPTLGPNAQKAAQMAEMSINEQTQSTVEENIKDFLE
                     KHAF"
     gene            complement(6215..7084)
                     /locus_tag="PLU28_03820"
     CDS             complement(6215..7084)
                     /locus_tag="PLU28_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG34226.1"
                     /translation="MIEQLAIVSGKGGTGKTTLCTALGGLFKNIIIADCDVDAANTAL
                     IFKTDLIKTYDYIGSRKAFIDHGKCISCFACVEKCRFGAITQKNEKPFINSISCEGCG
                     LCAKICPADAISFSVEKAGEYYISKEGNITLVHAQLYPGEDTSGKLIAQVRKTALELA
                     QTENYSMILIDGSPGIGCAAISSITAVNHVIIVTESTLSGLHDLKRIVSVTEKLRRPF
                     WVVINKSDLNPAITEQISKYCESLNIKVLGCIPYDKTVVKAIQEEKSILEYDCPARNS
                     VIEIYEKLRGEIL"
     gene            complement(7081..7920)
                     /locus_tag="PLU28_03825"
     CDS             complement(7081..7920)
                     /locus_tag="PLU28_03825"
                     /inference="COORDINATES: protein motif:HMM:NF025001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG34227.1"
                     /translation="MKIAVLSGKGGTGKTFISTNLAACIAKRHKVTLLDCDTEEPNCS
                     IFIKSKIDEKTEISLKIPHIDSSACSRCKKCSDNCYYGAISIFKQSHMIFDFLCHGCG
                     LCSYICPCNAIKESEKPIGIINSYIEKENITLHEGLLNIGQASGVPIIREIKKKAADS
                     EIIILDSPPGSSCSVVETIKGIDMALLVTEPTPYGLHDVDAVVNLCTDMRIPFVVIIN
                     KDCGSYKKMDLYFAEKDIRVVSRIPFSEEISEQSSTGRLIIDGKSSFHIFESICKSIG
                     VIS"
     gene            8120..8503
                     /locus_tag="PLU28_03830"
     CDS             8120..8503
                     /locus_tag="PLU28_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOG34228.1"
                     /translation="MIKVAIPLLENKGKDSPISEHFGHAPFFGFVYLEGSEYKVDIHD
                     NPFNEHTPGQIPQYMKQNGVNVMVARGIGSRAIEFFADLGIEVYKGADGTVQQIMDQF
                     IQSTLKDKDYKCNHSEDEHHDCHNK"
     gene            complement(8644..9024)
                     /locus_tag="PLU28_03835"
     CDS             complement(8644..9024)
                     /locus_tag="PLU28_03835"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOG34229.1"
                     /translation="MPRKKCLSNSGKGWWLSSVILLLIAEKSCHGYELVEKLKEFEID
                     LQGVGNIARVYTFLNMFEDSQYVKADWDTSFSPPRKVYAITSKGIEQLSVIKSEMFAQ
                     KNLVELYIERFSDLEKNNSHDKKL"
     gene            complement(9027..9314)
                     /locus_tag="PLU28_03840"
     CDS             complement(9027..9314)
                     /locus_tag="PLU28_03840"
                     /inference="COORDINATES: protein motif:HMM:NF021386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HOG34230.1"
                     /translation="MNAEKIDELRMQLKNFMDKDFKITSYPAKYSLKEKVIDYIFYLM
                     PCEGQYTEKEINALINELHTFGDPALLRRELVEHGYLKRTPDGSKYFIKER"
     gene            complement(9311..10531)
                     /gene="dinB"
                     /locus_tag="PLU28_03845"
     CDS             complement(9311..10531)
                     /gene="dinB"
                     /locus_tag="PLU28_03845"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HOG34231.1"
                     /translation="MKKTKKVKNESDLMPERKIIHVDMDAFFASVEIRDNPSLKGKPV
                     IIGAKPQERGVVSTCSYEARKFGVKSAMSIKTAYKLCPQGVYMHPDIRKYEKVSEELR
                     KIFYRYSFKIEPASLDEAYLDVSQTADLFSGEHNTALKIKNDIKTELCLSASVGIGYN
                     MLTAKLASEEKKPDGLFWIKNQTDFNNIFMNRPIGCLYGIGKATVKLLESYGIYLISD
                     LQSSTIEFLTSVLGSRGEEIYLACRGIDEREVTPSYQSKSEGREITFQYDTRDFKLIN
                     DTIINIIKELAFNLNEQKLWCRTLTLKIKYKDMHTITRAKTLDNPTNSSKEIYESIIR
                     LYNSAPKNQPIRLIGVSLSHFCDSPSIQLSFSDINHSQKDEKFNRLVHELKKKYGQEV
                     IKDPLDFKRYGGTV"
     gene            complement(10497..>12007)
                     /locus_tag="PLU28_03850"
     CDS             complement(10497..>12007)
                     /locus_tag="PLU28_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - exoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="VacB/RNase II family 3'-5' exoribonuclease"
                     /protein_id="HOG34232.1"
                     /translation="RKEIIFTIDGDDAKDFDDAVQIDKLPNGNFILGVHIADVSHYVP
                     EGTELDKEAFERGTSVYLIDNVIPMLPHELSDWMCSLVEKQDRLTMSLIMEIDKSGNV
                     IDYSVYNGIINSVKRLTYSKVNKILNKSAEPELVDEIGWLEPKFILMKELMELLRNNR
                     KERGAITAIEGAEVYFEFDEQGRVKDIIPRERGISEVIIEEFMIKANETVASIFDNQG
                     LPFIYRVHETPDPEMIVQFRNYVDAAGVKIKMPKKLHPKVLEEVLEKTKDSPLYESIQ
                     RLLVRSLKRAVYSDVNIGHFGLASESYTHFTSPIRRYPDLIVHRLLKRFIENKYSFSK
                     KENEKYAKILPNIANHSSKRERIANEAEWDLDDMKKAEYISNYLNVPFEVVVTSVTKF
                     GIFVEIPKKMINGLIHISQLKEYYRYDEKNNRLVSENSSSIISIGDKMAAAAVKTDKL
                     TGQIDFIPYEENKKNNSSTQKVHKSKKNPEKKDAPHKKIRMGEKNKKSKKRK"
BASE COUNT         3796 a         2024 c         2228 g         3959 t
ORIGIN      
        1 gtatctaaaa gacaacggta ttcaggtcaa gagtataagt tccttcacag gctttgaaga
       61 gatactggat ggaagggtta aaacacttca tcctaaagtt tatggaggga ttctggcaat
      121 aagagataaa caagagcata tgcaggaact acaatcaatg gacatcaaga caattgatat
      181 ggttgtagta aatctgtatc ctttttttga aaaggcagtg caggatattt cttttgaaga
      241 taaaattgag tttatagata ttggcggacc atctatgctt agatcggccg ctaaaaattt
      301 caaggacgtt attgtacttt cagatacaac agattattcg gtagttattg agcagcttaa
      361 aaataatggt gatgtcgaac aaaaaatccg tcgggagctt gcagctaaag cttttaatct
      421 taccagtgca tacgatgctg caatatcctc gtttcttacg ggaaaagagt ttcctgagta
      481 tctttcactt tcttatactt taaaacagaa gcttcggtat ggcgaaaatc ctcatcaaaa
      541 gtctgtttta tatgccagaa ctgataaaaa aggtcttata aacaattgtg aacagatttc
      601 cggaaaagag ttatcataca ataatataag ggatatggat gttgcataca aacttgtaac
      661 tgaatttgac gaaccggcat gttgcgcggt caaacacaat tcgccatgtg gagctgcagt
      721 tgcacaaaac atatacgatg catatactca agtctatgag tgtgatccag tatccatatt
      781 cggaggaata gttgctttta atcgtccggt agatttaaaa actgctgaaa agcttcatga
      841 gatctttctt gaaataatta ttgcccctga ttttgagcca gaggctttgt tgctgcttaa
      901 aagcaaaaaa aatcttcgaa taataaagtg tgattgtact atttcagata agcttgaaat
      961 gataactgta gacggcggag tcttggttca gagtatagac agagatttta aagaagagta
     1021 taaagttgtt actaaaactt cacctaccca ggaacagtta agacagatga aatttggaat
     1081 gattgtggct aagtatacga aatccaacgc catagttacg gtaaaacaga tgcatacagc
     1141 ctcaattgcg ggaggacagg tgaacagaat atggcctgcc gttcaggcga tgcagagagg
     1201 aaagggagcc caattactat gttctgatgc tttttttcca tttgatgata ttgttcaggc
     1261 ggcttacgag tatggtataa aagctataat gcagcctgga ggttcggtaa acgacaaagc
     1321 ctcgatagaa ctttgtgaca agtatggtat aagtatggtt tttaccggaa tcagacattt
     1381 taaacattaa aatggagagt gaagaatgaa ggtcatgata ataggttcag gaggaagaga
     1441 gcatgcttta gcccataagc tttctcagag tcctgatatt gattgcatat actgtgttcc
     1501 cggcaatggc ggaacagctc ttgaagacaa atgctttaat gtagagctaa ataatattga
     1561 agaaatatct gcttttgcat tgggaaaaaa tattgattat acttttgttg gaccggaagc
     1621 atatcttgtt cagggcataa ctgatatatt ctctcaaaga aaacttaaga taatagggcc
     1681 tgataaaaaa gcatccatgc ttgaatccag caagtcttac gcaaaagagt tcatgaaaaa
     1741 atacaatgta cgcactgccg cttatgatac tttcagcgag tatgagaaag ctatagatta
     1801 catattgaaa gcagattttc ctttagttat aaaagcagac ggattagctg gagggaaggg
     1861 agtagttatt gctcagaatt atgaacaagc tgtggaaacc ttaaattttt ttatgaaaga
     1921 ggatattttt aagggatctg gcaaaacagt agttatagag gaatttttaa agggatttga
     1981 aacttctgtt ctggcagcgg ttgatggaaa aacgatactt ccgttcataa gtgcaaagga
     2041 tcataaacag gcttacgatg gaaacaaagg accaaatacc ggcggaatgg gagccatagc
     2101 tcctaaccct ctttttacag aggagcattt tgaagatttc aaaaacaata ttatgttgcc
     2161 taccctaaga ggaatacggg aagaaaagct taactataaa ggaatcatat tttttggact
     2221 catgatatgt actaaaggtg tttaccttct cgaatacaat gtaagaatgg gagatcctga
     2281 aactcaggca gttcttccgc ttatggaaac agattttttc caggtaataa aacatatata
     2341 tgatggcagt cttgacggat atgaaataaa atggaaaaaa ggttcgtgtt gctgtgttgt
     2401 tgcggcaagt catggctatc caggaaaata cgaaactggg tttgaaataa ccggctatga
     2461 tcaagtagct tcaaaagtat actcagccgg aactgttttt aaaaatggaa aactaatatc
     2521 atctggcgga agggtactga atatatcgtc tttgggagat aacctttttc aggctcggca
     2581 aaaggcatat atggacttgg caaaaataaa gtttgaaaac atatactata gaaacgatat
     2641 cggaggctaa aatgatagaa agatatgcta tgcaaccgct taaagatcta tggaacgaag
     2701 aaaaccagta taaaaggtgg ctcgaggttg aagtatctgt tgtaaaagct tttgaaaaat
     2761 acgggcaggc tcctgtcggc acatcgcaac agatactttc aaaagcagtt atagatgtgc
     2821 agaagattca tgagattgaa gctgtagttg atcatgacgt gattgctttt ataaactgtg
     2881 ttactcagaa gatgggagat gaagcaagat actttcacaa aggaatgact tcttcagatg
     2941 tagtagatac agcactttca cttgctttaa aaagatcagg tcttgtaata aagcaggagt
     3001 tggaaacatt tgaagaggtt ctgaaagaac aagcatttaa gtataaagat actattacta
     3061 ttggcagaac acacggaatt catgccgagc caacatcatt tggccttaag cttctttctt
     3121 atcttgctga agttcaaaga aataaagtga ggctggatgc tgctatagaa aatatttcat
     3181 acgccaagat aagcggtgcg gttggaaatt acgcaaatat tgatcctgag atagaaagtc
     3241 tggcattaca gatgatagga cttaagccat gcaaagtatc tactcaaatt gttcccaggg
     3301 atatacatgc ggaatttctt aatactctgg cacttatagg ggcaaatata gaaagacttg
     3361 cgacagagat aagacacttg cagaaaactg agattctcga agtacaggaa ccatttaaaa
     3421 agggtcagcg tggatcatcg gccatgccac ataaaaagaa tcctattctt tgtgagagac
     3481 tttgcggaat gtcacgaatg cttagaagct atactatagc aggatatgag gacacagttt
     3541 tgtggcatga aagggatatt tcccattcaa gtgttgagag aatggttttt ccggatgcaa
     3601 ccatgattac ttattatatg ataaataagt ctgtatggct ggtaaaaaat ctggttgtat
     3661 acgaaaataa gatgaaagag aacttcaaag catctcacaa tcttgttttc tctcaaagac
     3721 ttatgcttgc catgatagac aagggattct caagagaaga aagctataag cttattcagg
     3781 aactttccat gagatcatgg aacgaatcta tagatcttat gcaggtagtc cttaatgatg
     3841 aaagaacatc atctaaattc aatgaaaatg agattaaaga gatatttgat cctgcgtact
     3901 atttgagaaa tattgaagaa gtctttaaaa ggtttatata ataaaaaatg atccggtgaa
     3961 atatttccgg atcatttttt tatacttctg gcaattgagg taatcctctt tacaaaatca
     4021 gttttacctt ttgtgtaagc ttcacggtca tttttataga ttttttcaag agtaagtttt
     4081 aagttttcat actctctgca ggtctgagga tttttaatga gataatttct aaaatagaac
     4141 tcatcccaat cattcatata tcttttacgt gaacatgaaa agcttgtccc ttaaagcctt
     4201 gaggtgtata tcctttcatg aacatcatat gcgggggagg attatccggc tgatggctat
     4261 agctgtaacc tgcgccgata atcctatgaa tgagatcaga agtatcggtt ttattatcta
     4321 tttcaataag gatatctact gttggcttag ccttcatacc tggaacagag gtgcttccaa
     4381 tatggcttat acggagtata tccttttggg gaatatgttt cttaagattt tctttttctt
     4441 tttcaaaaag ctttttccat tcaggattat aatcagaaat tattattggg aaaagctttc
     4501 cgagttcctc tgttgaaagt tcgtttattt tttcatcgta tttgcgtctc cttgtctttt
     4561 tcttgattat atagaaaaaa cttttcttta tagatatttg acattacaaa aacttatgat
     4621 attattaaaa tgaataaaca tgttttaaaa agatgtcatt aagatatttt tctctgagaa
     4681 aagataatat agttatctgc attggctcta aataatactg ttttttcaag gaggtgcata
     4741 atgaatatat agattaaacc gtttcttact gcttcttttg atttaaaaaa tctattaata
     4801 aaaaggaggc aaaagatgaa gaagaaaact attaacttta aagaaaaaga caaaaattat
     4861 ggcaattcgc aggtaaaaga aagtaacagg attactccta aaaagactac acgaaactta
     4921 ggtgctggtc ttcgtcagag taaaaaaggc taagaaaggt tgataattaa tttcatggtt
     4981 ctaagaatct tatgattctt agaaccattt tttcgtaaaa agccatataa cttaattata
     5041 aatcatttta aaattacaca atgtatttgt ttgttaaaat taatttatag gtaaaaaaca
     5101 attttataat tatatgatat aatctaatta caataacata aaggaggaaa aaacatggaa
     5161 gatatgaata taacacgaat gcctcttata ggtgacaaag caccagagtt taaagccaaa
     5221 acaacacagg gagatataaa ctttcctcag gattacaagg gtaaatgggt aattttattt
     5281 tctcatcctg cggatttcac tccagtatgt acaacagaat ttatgacttt tgcaacaatg
     5341 gcagatgagt ttagggctct taataccgag cttgtagggc tttcagtcga ttctttatat
     5401 gcacatattg catggctgag aaaaatacag gaaatagaat ggaatggaat gaaacatgta
     5461 gaagtaaaat ttcctcttat tgaagatata aagatggatg ttgcacacaa gtacggtatg
     5521 atacagcctg gacagtcaag tacccaagcc gttagagcag tctttataat agatccaaat
     5581 gcagtaataa gaaccattct ttactatcct ctttccacgg gaagaaattt cgatgaaata
     5641 aagagaataa ttcttgctct tcaaaaagct gataacgata aagttgcgac tccggctaac
     5701 tggaaacctg gagatgatgt catagttccc acagcgggat cttgtggagt ggctaaagag
     5761 agaatggaaa gcaaaagtga agatacatac tgtcttgatt ggtttatgtg ttttaaaaaa
     5821 gagaaaaaat aattaatata ataaaagaga tgaggaaatc ctcatctctt ttattatgtg
     5881 tacagcagcg ttaaaagtta aaaggcatgt ttttctaaaa aatcttttat gttttcctca
     5941 actgttgatt gggtctgttc atttattgac atttcggcca tttgagcagc tttttgggca
     6001 ttcggcccta atgttggagc tatcagaact gttacacctt tttcagcaaa gaattctatt
     6061 acctttggtc cggcaccatg ttcaacatca aaaggatttt taaaaaaagt atattctgat
     6121 gttgaactgt tatacacaac aaaaaatgac gctcttgcaa atctgtcatc aacctgggaa
     6181 cttttggttt cgtgttttgc gcataatcct attttcatag tatctctcct ctaagttttt
     6241 catagatttc aattacagaa tttctagctg gacaatcgta ttctaaaata ctcttttctt
     6301 cctgaattgc ttttacaaca gttttatcat atggtatgca ccctagaact tttatgttca
     6361 gactttcaca gtatttgctt atttgttcag ttattgcagg attaagatcg gatttgttta
     6421 tgacaaccca aaaaggtctt cggagctttt ctgttacaga aacaatcctt ttcaaatcat
     6481 gcaatccaga taaggtggat tctgtaacta taatcacatg atttacagca gtaatacttg
     6541 aaattgcggc acatcctatt cctggtgaac catcaattaa tatcatactg taattttccg
     6601 tctgggctag ttcaagtgct gtttttctga cctgcgcaat aagctttccg gaagtatctt
     6661 ctcccgggta gagctgagca tgaaccaaag taatatttcc ttccttgctg atataatatt
     6721 ctccggcttt ttctactgaa aaggatatag cgtcagcagg acatattttt gcgcataaac
     6781 cgcagccttc acatgaaata ctgttaataa aaggcttttc gtttttttgg gttattgccc
     6841 cgaatctaca tttctcaaca catgcaaagc atgaaataca ttttccatgg tcgataaaag
     6901 cttttcttga gcctatataa tcataggttt ttataaggtc agtcttaaat atgagagcgg
     6961 tattggcggc atcaacatca cagtctgcaa ttattatatt tttgaatagt cctcctaatg
     7021 cagtacataa agtagttttt cctgttccac cttttccgct tactattgca agctgttcta
     7081 tcatgatatc actccaatgg acttacagat ggattcaaaa atatggaatg aactttttcc
     7141 atcaattatc aatcttccgg ttgaactttg ttctgatatt tcttctgaaa aaggtattct
     7201 tgatactact cttatgtctt tctcagcaaa atagagatcc atctttttat atgatccaca
     7261 gtctttattt attattacca caaaaggtat tctcatatct gtgcataagt ttactacagc
     7321 atcaacatcg tgtaggccat aaggtgtagg ttctgttacc aataatgcca tgtctatgcc
     7381 tttgatagtt tctaccaccg aacatgaaga acccggagga gaatcaagta taattatttc
     7441 agaatcagct gctttttttt ttatttcgcg gatgataggt acaccgcttg cctgacctat
     7501 atttagtaga ccttcatgaa gagtaatatt ttctttctca atataactat ttataattcc
     7561 tatgggtttt tcagattctt ttatggcatt gcaagggcag atataagaac ataaaccgca
     7621 tccgtggcat aaaaaatcaa aaatcatatg actttgctta aagatactta ttgctccgta
     7681 ataacagttg tcagaacatt tcttgcatct gctgcaggca gaactgtcaa tatgcggtat
     7741 cttcaaggat atttctgttt tttcatcaat ttttgatttt ataaatattg aacagttagg
     7801 ctcttcggta tcacaatcca agagggtaac tttatgtctt tttgcgatgc aggcagctag
     7861 gttggttgat ataaaagtct ttcctgtccc gccttttcct gataacactg caattttcat
     7921 atttttctcc ttaatgtagt atttttcaga ttcaattata tataaaaaaa atatatatat
     7981 caataatata aaagaatgat tttggattat aacaattatt aaaagtataa aatcagctat
     8041 aatcactatt aatcaaaaat ggtaaatata taaaactatg atatagttat agataagaaa
     8101 aagaacggag gtaatcagta tgataaaagt tgctatccca ttgcttgaaa acaagggaaa
     8161 ggattcacca ataagtgaac atttcggaca tgcacctttt tttgggtttg tgtatctgga
     8221 gggatccgaa tacaaagtag atattcatga taaccctttt aatgagcata caccaggtca
     8281 gattccgcag tatatgaaac agaatggcgt taacgttatg gttgcgaggg gaataggttc
     8341 aagggcaata gaattttttg ctgatctggg aattgaagtt tataaaggtg cagatggaac
     8401 tgtacagcag ataatggatc agttcattca aagtacgctt aaggacaagg attataaatg
     8461 caatcattcg gaagatgaac accacgactg tcataacaaa taaaaggctg cggcctttta
     8521 tttttttagc aagacggtat atctcaatgt tgaacagctt actttgacaa acgggagtat
     8581 gtaatcaagt tacagtatcc tgaagaaaaa taaagcaggc ggttttctct taaaccgcct
     8641 gtttcaaagt tttttatcgt gactgttatt tttttcaaga tcagaaaaac gttcaatata
     8701 tagttctaca agattttttt gtgcaaacat ctcacttttt ataactgaga gctgttcaat
     8761 gcccttggaa gttatagcgt atacttttct tggcggacta aaagaagtat cccaatctgc
     8821 ttttacatat tgtgaatctt caaacatatt taaaaaagta taaactcttg caatatttcc
     8881 tactccctga agatcaattt caaattcttt aagtttttcc accagttcgt agccatggca
     8941 gctcttttct gcaatcaaaa gaagaatcac cgaagaaagc caccagcctt ttcctgagtt
     9001 acttaagcat tttttcctag gcattttcaa cgctccttta taaaatattt agaaccatcc
     9061 ggagttcttt taagatatcc gtgctcaacc agttctcttc gtaaaagggc aggatcaccg
     9121 aaggtatgta gttcatttat aagagcattt atctcctttt cagtgtactg accttcacag
     9181 ggcataagat aaaaaatata atcgattact ttttctttta ggctgtactt tgcaggatag
     9241 gaagttattt tgaaatcctt gtccatgaag ttcttaagct gcatgcggag ctcatctatt
     9301 ttttctgcat tcatactgtg cctccgtatc ttttaaaatc aagaggatct ttgataacct
     9361 cctgtccgta cttcttttta agttcatgca cgagcctatt gaatttttca tctttctggg
     9421 aatggtttat atcagaaaaa gaaagctgta ttgatggaga atcacagaaa tgactcaaac
     9481 tcacaccaat aagacgtata ggctgatttt taggagcaga attataaagc ctgattatac
     9541 tttcgtatat ttcttttgaa ctattcgtcg gattatcaag ggttttggct ctcgtgatgg
     9601 tatgcatgtc cttatatttt atttttagag ttaacgtcct gcaccagagt ttttgttcat
     9661 ttaggttaaa tgcaagttct tttattatat ttattatagt atcgtttatg agtttaaaat
     9721 cacgtgtgtc gtactgaaaa gttatttctc gtccttcaga tttgctctga taggaaggag
     9781 ttacttcacg ttcatctatt cccctgcatg caaggtaaat ttcttcccct cttgatccca
     9841 aaacagatgt aagaaattct atagtagagc tttgaagatc agatattaga taaattccgt
     9901 aactttccaa aagctttaca gtggctttac ctattccgta aagacagcct ataggcctgt
     9961 tcataaaaat attgttgaag tctgtctgat ttttaatcca gaaaagacca tctggctttt
    10021 tttcttcact tgcaagttta gctgttagca tattatatcc tataccgaca gatgcactga
    10081 gacatagctc agttttgata tcattcttta tttttaaagc tgtattatgt tcgcctgaaa
    10141 aaagatcagc agtctgagat acatcaagat atgcttcatc aagtgatgcc ggttcaattt
    10201 taaaagaata cctgtaaaat attttccgca gttcctcgga taccttttcg tattttcgta
    10261 tgtccggatg catatatact ccctgtgggc atagcttata agctgttttt atgctcattg
    10321 cagactttac tccgaatttg cgagcttcat acgaacaagt agaaactact ccgcgttctt
    10381 gcggtttagc acctattata actggtttcc cctttaatga aggattatct ctaatctcaa
    10441 cgcttgcaaa aaacgcgtcc atatctacat gaataatctt tctttctggc ataagatcac
    10501 tttcgttttt tacttttttt gtttttttca cccattctta tttttttatg aggggcatct
    10561 tttttttctg gattcttttt acttttatgt actttttgag tagacgagtt gtttttttta
    10621 ttttcttcat aaggtataaa gtcaatctgt ccggtaagtt tatcggtttt tactgcggct
    10681 gcagccatct tatcgcctat actgataatt gaggatgagt tttctgaaac tagcctattg
    10741 tttttttcat cataccggta gtactcctta agctgcgata tatggatgag accatttatc
    10801 atttttttag gtatttcaac aaagattcca aacttggtta cagaagttac aactacttca
    10861 aagggtacat tcaagtagtt ggatatgtac tccgcttttt tcatgtcatc aagatcccat
    10921 tcagcttcat tggcaattct ttctcttttg gatgaatgat ttgcaatatt aggaaggatc
    10981 ttggcatact tttcgttctc tttttttgaa aagctgtatt tattttctat aaatcttttt
    11041 agaagtctgt gcactataag gtcgggatac ctacgtatcg gagatgtaaa atgggtataa
    11101 gactcggatg caagaccaaa gtgaccaata tttacatctg aatatactgc acgttttaag
    11161 gatcttacaa gaagtctctg tatactttca taaagtggag aatcttttgt cttttccaag
    11221 acttcctcca gaacttttgg gtgaagtttt ttaggcatct ttatttttac acctgcagca
    11281 tcaacatagt ttctaaactg tacgatcatt tctggatcag gcgtttcatg caccctgtat
    11341 atgaaaggaa gaccttgatt atcgaatatt gaggctacgg tctcgttggc ctttatcata
    11401 aactcttcaa taattacttc ggagattccg cgttccctag ggatgatgtc ttttactctt
    11461 ccttgctcat caaattcaaa gtaaacttcg gctccttcga tagcggttat ggcaccacgt
    11521 tcttttctgt tgtttcgtag aagttccatc agctctttca taagtatgaa ttttggttcc
    11581 agccaaccga tttcatcaac cagctcaggt tcggcacttt tgttaagaat tttatttacc
    11641 tttgagtatg taagccgctt gacactgttg attataccgt tgtaaacact gtaatctata
    11701 acatttccgg atttatctat ctccattata agtgacattg taagcctgtc ctgtttttct
    11761 accagagaac acatccagtc tgagagttca tgcggaagca taggaattac attatctata
    11821 agatagacac tggtgcctct ttcaaaagcc tctttatcca attctgtacc ttctggaaca
    11881 taatgcgaga catctgcgat atggactccc aagataaaat tgccgtttgg aagtttgtct
    11941 atctggaccg catcatcaaa gtctttagca tcatcaccat cgattgtaaa tattatttcc
    12001 tttctga
//
LOCUS       DAOSHR010000045        11991 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_4499_length_11991_cov_5.924263, whole genome shotgun sequence.
ACCESSION   DAOSHR010000045 DAOSHR010000000
VERSION     DAOSHR010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 11991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11991
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4499_length_11991_cov_5.924263"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..537)
                     /locus_tag="PLU28_03855"
     CDS             complement(1..537)
                     /locus_tag="PLU28_03855"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HOG34233.1"
                     /translation="MRAFKGQKTERTIFVHYTDSPLGKLFLGATEDSIVFLKFHNELP
                     EQHNRIERTILYKTGSPLLEKMELQLNEYFRGKRREFDLPIYLEGTDFQNKVWKSLMD
                     IHYGETITYSAQAQKIDSLSAIRAVANANGKNPIPIIVPCHRVIGTNGKLTGYSGGLW
                     RKKFLIELEKEISAQKLF"
     gene            660..2465
                     /locus_tag="PLU28_03860"
     CDS             660..2465
                     /locus_tag="PLU28_03860"
                     /inference="COORDINATES: protein motif:HMM:NF021615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HOG34234.1"
                     /translation="MTEKIKLKTKRILRSSLKSLKNNFLEYFIFESIYKTALHFLIIP
                     LMIFIFTFSVKLSNDGTIKNTEFFKLFLDIKGFSGIFIVSLFLSVIFFIELFVLVIIN
                     YSARNNSNPSVIHAFITSLKKLRLLFGKGFFIMLVFALLIIPFLETGIESGIIANIRI
                     PDFVDEYIFSTYKLFIPYIILMLMIVSFSVGLIFMINVAVEEDCTLQQAFKKSRLLAK
                     GNKIKIIWIIFIWNLIILAIPAFLIGVPFILLVLLLIRATGFDYNEFTLTMPYIYAWA
                     VQLYYIVISMYVPANICLISEIYHALDQEPKEFKLSVPQKIRKFQMKVKRVLSSYSLV
                     FLSLILTSLFFYTLYSADRFKNSMFNFTGVDIISHRGDSKNFTENTLQAIMSAKDMGA
                     DSCEIDVQLTKDSRVVVFHDKSLKRLTGISEYVNNLDYSELIALAGQYVKIPLLEDVL
                     DYAKDNIKLVIELKPDSRRTELVEKVVGYVKNRNMVSKIMIQSLDYKSLEMIEIIEPR
                     IITGFIAYMLYGDYKKLVVDFLNVEKSYLSEKMIKDIHNADKKVWVWTVNSRDEMENF
                     VDMKVDGILTDDIMTLKEYLKEKSEEVLKYANIIK"
     gene            2709..3305
                     /locus_tag="PLU28_03865"
     CDS             2709..3305
                     /locus_tag="PLU28_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34235.1"
                     /translation="MKRILITILIVLSVMTIFGNPKDLIKNANYDKLKTLIDIRIEKE
                     RYKVTPIDKERLEAILPYIVYLSNSYSIDPVIFITIIEQESGYRWITGDSGKAAGFVQ
                     LHLSTAWYCMDKFKNELIDMGLTNTEIKSIKDLEMSVIRTSVLGFYWFVLKLKETGSY
                     RKAIGLYNGVDNESYINKFVEKYMQLHEIYFNLKGYSA"
     gene            complement(3398..4081)
                     /locus_tag="PLU28_03870"
     CDS             complement(3398..4081)
                     /locus_tag="PLU28_03870"
                     /inference="COORDINATES: protein motif:HMM:NF021485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2202 domain-containing protein"
                     /protein_id="HOG34236.1"
                     /translation="MKKVLLMAGVLSAVIFSFAQGPQMGKNPGNLPVKRQADKGYTSQ
                     RDLIQDQTLKNYVGLFAFQTVDETEKTALLKMVEEEKLARDVYIALAEKWDINPFINI
                     AKAEQVHMQQVQALLEKYKIDYVAQEEGKFSSQDVQELYNQLVQQGSTSQIEAFKVGM
                     LIEEMDIADINSEIVKADNEDIKQIFVNLRQGSENHLMAFNRQLLINNEKYQPKYLSQ
                     SEIEKILGR"
     gene            complement(4210..6858)
                     /locus_tag="PLU28_03875"
     CDS             complement(4210..6858)
                     /locus_tag="PLU28_03875"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HOG34237.1"
                     /translation="MADWYRKTVEETLKELNTDITTGLSYKQAEERLNSYGKNELVDG
                     ALKKPWKIIMEQASGIMVIMLVIAGIISFFLGETTDAIVILAIVVINILLGFSQEYKA
                     EKAMAALRKMSLPTVKVKREGKLIEISSKELVPGDIIQIDAGNSIPADCRIIQSVNLK
                     VQESALTGESEPIEKMSDVISDVNLSLGDRKNTGYMGTVSTSGRGTAVVTQTGMNTEL
                     GHIAKMMQSVDRQPTPLQKKLDQLGKLLAVIALAIVAIVFVLGIFRGEDIQVLFMTAI
                     SMAVAAVPEGLPALVTISLALGTQRLLKRNALIRKLLAVETLGSVSVICSDKTGTLTQ
                     NKMTVTSIYTSTLGAADINGLKVSSPDIKMLLTAGLLCNDSEIQENGKMIGDPTEGAL
                     VEIAQRNGYDKRKLESLLPRINELAFDSDRKMMTTLHLVQNIKEVSEDLYGILKEFKY
                     ISFTKGAADSLLKFADRILDGRLIRPITQNDRDRLMAVNDSLARKGVRVLAAAFRPAE
                     ASEKVYEDSLILIGLFGMIDPPRPEVKAAVEKCRSAGIKPVMITGDHPMTALYIAKEL
                     SITQSDTVVTGSELEKMTVEELEKQVESTSVYARVSPEHKLKIVQAWQNKGYTAAMTG
                     DGVNDAPALKKSNIGVAMGITGTDVSKQAADMVLLDDNFSTIVSSVEEGRAIYDNIIK
                     FIKYILSSNFAEILLMLAAPFIGMPLPLLPLQILWINLVTDGLPGLALGVEPAEKDIM
                     KRPPRNNKGGVISTGLLINIIIVGIIIGAASIVTGHIYYSNNSPVWQTMIFMIVTISQ
                     MFYAYSVRSSSVSVFKTGLWSNKYLLGAVLLTVALQLIVVYIPALSSIFGICALSFKD
                     LLIAFGVSSIVLWYDETRKFFIRRSR"
     gene            complement(7147..9132)
                     /locus_tag="PLU28_03880"
     CDS             complement(7147..9132)
                     /locus_tag="PLU28_03880"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOG34238.1"
                     /translation="MKKSLKTLMIFLILCVSVLPLVILTVVTSISSYQSDYRGAENYL
                     KVANSLKAKTIEDSIAPISKAVDFMSQNNSLRLYEKNINDSTLFIDDFRKLKSTFPEI
                     QLFFVGYEDKSIFFYPHNEVDSSYNPKERSWYIQAVANAGTTVTTDPYVDTVTKKMVV
                     SVVKTFTGNTGALKGVAGADIAIDSLLTEISANNSFRSSYSFVADAAGKIVLHSDNSM
                     MGKSISEESYFSKVKENQGVLGEKIGDQDKIICYNKISSLGWTVFTLIDRAEVLEQAK
                     RTLILLIVFSVAITAIALIITIFFIRFNVSRPMERILSEIKSFGKGDLTTKFTPSGSL
                     EIVVMAQNLNTMAKELDESMKSILQASKQLNASSSDLAAVAQESSSTSEELSAQSDVI
                     KNNVQSASANIEELNASVEEVASSAQSVSKASVELASNADNVSKSVSSGMKNVQGIIS
                     TIQETVGQTESTSKLVADLAESSRNVETILQSINSITEQTNLLALNAAIEAARAGEAG
                     KGFAVVADEIRKLAEESKKATQDISDILTDIRKKTELANQATSLTKSKVENVSEGGKN
                     ISINFKEIETLVGNINSMIENLSASAQEQSAASEEISSAMQNASKIMLKINEEINNMV
                     TGVNQQSEGAQQISANSEELNALSENLETQTQKFKTT"
     gene            complement(9358..10512)
                     /locus_tag="PLU28_03885"
     CDS             complement(9358..10512)
                     /locus_tag="PLU28_03885"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOG34239.1"
                     /translation="MTDNIFYTAPHFEQDIVKAKDEYMYDSSGKEYIDFESGIWCCAL
                     GHSNDRVNQAIKDQLDKVTHLHTKLVNSVSKKAADDLLRITGIKKGKAVFLTSGSEAV
                     EMGLKLSFAATDRKKAIMFSNSYYSALGTTGNYKNEECIIKKDFTAYDENEPESFFSG
                     IDFSQVACCIFELGSTSGRVLFPHEKLVRELSQRVKSSGGYVISNEVTTGYGRTGKYF
                     GFQHYDIMPDIVCMGKGIGNGYPVSAIVMTDIVASAMAGKSIGHVQSHQNDPLGGAVV
                     SAVIKEFEERDLIRASANKGKLLSKELMTLKEKFQCIKDVRGRGMMHAVEFDQKILVA
                     DISAMMFEKGFLIGANGLYNVLRFDPCLIINEESILKMTKELEKILNQLM"
     gene            complement(10532..>11991)
                     /locus_tag="PLU28_03890"
     CDS             complement(10532..>11991)
                     /locus_tag="PLU28_03890"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOG34240.1"
                     /translation="RTLGTPIYQQIVEKMRELIDNGDLRPGEKLPSTRELAEKLGVNR
                     STVYLAYEELWVTGYIQSNPGSYTFVRRKRAYPLKNEPSIKTAIDWNTCIDKRINELY
                     TRDANSYEKTLKFPREYLDMKSISPDERLYPVKTFKVAFNAAVKENMSKMFSYSDIKG
                     YLPLRTFLSAHLKKHGIQADINEIIITNGSQNSLDLITRLLLSDGSTVAAESPTYASL
                     YNFLRMRRCNVQELSLNETGGYDFSFLEKCEKPAFFYTTPNFQNPTGYTMSQQDRENL
                     LKKCQELRVPIIEDGFEEEMKYFGKSPLSIKAMDTSKTVIYTGSFSKVLFPGMRIGWI
                     VADKPLIDRITFLKNCSDISSGQIMQAAAFEFCQRGYFDEHIRKLKRVYKKRMSVFLS
                     QMDKVIQNPDVSWTKPLGGYTSMITFNNVNSNIQQVHQIFLDHGISPYNGNDFFFKPQ
                     EKIHFRLSIAKLNEEEIEEAVDSLSKVIHDVYLRH"
BASE COUNT         3477 a         2265 c         2172 g         4077 t
ORIGIN      
        1 ttaaaaaagt ttttgggcag agatctcttt ttcaagttct atcaggaatt ttttgcgcca
       61 tagtccgccg ctgtagcctg tcagcttccc gttagttcct attaccctgt gacacggaac
      121 aattatcggt atggggtttt ttccatttgc atttgcaact gcccttatag ctgacaggct
      181 gtctattttt tgtgcctgag ccgaatatgt tatggtttca ccgtaatgta tgtccataag
      241 gcttttccat actttatttt gaaaatctgt gccttcaagg taaatcggca gatcaaattc
      301 acgtctttta cctctgaagt attcattcag ctgcaattcc atcttttcaa gcaatggtga
      361 gccggttttg taaaggatag ttctttctat cctgttatgt tgttccggca gctcgttgtg
      421 aaatttcaga aatactatgg aatcttctgt tgcacctaaa aaaagctttc ccaacggtga
      481 atctgtgtaa tgaacaaaaa ttgttctctc cgtcttctgt cctttaaaag ctctcatttc
      541 tttctcccac cttaaaaaag taattgattt ttgctataat attgaagcaa aaaataattt
      601 tgatatttaa tagcattata tcagatttta tcggcagtaa ataaattttt ggaggcaaga
      661 tgacagaaaa aatcaaattg aaaacaaaac gtattttgcg gtcttcactg aaaagtctta
      721 aaaataattt tttggaatat tttatatttg aaagcatata taaaaccgca ctgcattttc
      781 tcataatacc gcttatgata ttcattttta ctttttcggt aaagctttcc aatgacggaa
      841 ctataaaaaa tacagagttc tttaaactat ttttagacat caaaggattt tcaggaattt
      901 ttatagtttc actttttttg tctgttatct tttttattga actttttgtt cttgttataa
      961 ttaactattc tgccagaaat aacagtaatc cttcggtaat ccatgctttt attacttcat
     1021 tgaaaaaact gcgacttctt tttgggaaag gattttttat aatgcttgtc tttgctctgc
     1081 tgataattcc atttcttgaa acaggtattg agagcggaat tattgcaaat atacgtattc
     1141 ctgattttgt ggatgagtat atcttctcaa cgtataagct ttttattcct tacataatac
     1201 ttatgctcat gattgtatcc ttttcagtag gactcatctt tatgattaat gttgctgttg
     1261 aagaagactg cacattacaa caggctttta aaaaaagccg tctgcttgca aaagggaaca
     1321 agataaagat tatctggata atttttatct ggaacttaat tatcctagct attccagcgt
     1381 ttttaatagg tgtgcctttt attcttttgg ttcttttact tataagagct acagggtttg
     1441 attacaatga gtttacattg accatgccct acatatatgc atgggcagta caactgtact
     1501 atatagttat ttcaatgtac gtgcctgcaa atatctgcct gatttcagag atataccacg
     1561 ctttagacca agagcccaaa gagtttaagc tttctgttcc gcagaaaatt agaaagtttc
     1621 agatgaaggt aaaaagagtt ttaagttctt attcattggt atttttatcg ttaattctta
     1681 cctctctctt tttctacacc ctttactctg cagacaggtt taaaaactca atgtttaact
     1741 ttacaggagt agatataata tctcataggg gagacagcaa aaactttacg gaaaataccc
     1801 ttcaggctat aatgtctgcg aaagatatgg gtgcggacag ttgtgagata gatgttcagc
     1861 ttacaaagga ttcaagggtt gttgtctttc acgataaaag tctgaaaaga ctcaccggaa
     1921 taagtgaata tgttaataat cttgactatt cagagcttat agcacttgcc ggacagtatg
     1981 taaagatacc tcttcttgaa gatgttcttg actatgcaaa ggataatatc aaacttgtaa
     2041 tagagctcaa accagacagc agaagaacag aactggttga aaaagtagtc gggtatgtga
     2101 aaaacagaaa tatggtatca aaaataatga tccaaagcct tgattataaa agtcttgaaa
     2161 tgattgaaat catagaacca agaataatta ctgggttcat agcttatatg ctttacgggg
     2221 actataaaaa acttgtggta gattttctga atgttgagaa atcatacctt tcagaaaaaa
     2281 tgataaaaga tattcacaat gcagataaaa aagtatgggt atggacagta aacagtagag
     2341 atgagatgga aaactttgtt gacatgaaag ttgatggtat tcttacagac gatatcatga
     2401 ctttaaagga gtatcttaag gaaaaaagcg aagaagtttt gaaatatgcg aatatcataa
     2461 aataaaaaag gttaattgcg acttctgtaa taaattaacc tatgtatttt atagttgttc
     2521 acagtattct gttaacaaag ataacttttt gtaaaaaaac aaaaatgtaa ctgtgagtaa
     2581 ataaaaagga tagagttttg gttgaaaagt taaatattat atgataatat gcctccagcc
     2641 ctgaaaaggg tttttgtatt catataatgt tattgtttca tatacctaaa ttaaggacgg
     2701 tgtctgtgtt gaaaagaatt ttgataacca tactcattgt tttatctgta atgacgattt
     2761 ttggaaatcc caaggatctc ataaaaaacg ccaattacga taaactcaaa actttgattg
     2821 atattaggat tgaaaaagaa agatacaagg ttacgcccat agataaagaa agacttgagg
     2881 ctattcttcc gtatattgtc tatctcagca atagttattc catagaccct gtgatattta
     2941 ttaccataat agaacaggaa tctggataca gatggataac aggcgacagc ggtaaggcag
     3001 ccggatttgt gcagcttcat ctttccactg catggtactg tatggacaag tttaagaatg
     3061 aactcattga tatgggactt actaatacag aaataaaaag cataaaagat ctggaaatgt
     3121 ctgtaataag aacgtctgtg cttggttttt actggttcgt tttaaagctt aaggagaccg
     3181 gttcttacag aaaggctata ggactttaca acggagtgga taatgaaagc tatataaaca
     3241 agtttgttga aaagtatatg cagctgcatg aaatatactt taatcttaaa gggtattctg
     3301 cataaaacgt aaaaattttt gttaacagac aactctgcaa ataagaagat ataaaacaag
     3361 acgctgaata aaatacttat tcagcgtctt gttttattta tcttcctagg attttttcta
     3421 tttcagattg tgatagatac ttgggctgat acttttcgtt gttaatgagc agttgtctgt
     3481 tgaatgccat tagatggttt tcggaaccct gtctaagatt tacaaagatc tgctttatat
     3541 cctcgttgtc agctttaact atctcactgt ttatatcagc tatatccatt tcttcaataa
     3601 gcattccgac tttgaaagct tcgatttgtg aagtgcttcc ctgttgtaca agctgattgt
     3661 ataactcctg tacatcctgg ctgctgaact taccctcctc ctgagccaca taatctattt
     3721 tatatttttc cagaagagct tgtacctgct gcatatgaac ctgttcagct tttgcaatgt
     3781 ttataaacgg atttatatcc catttttctg ctaaagctat gtaaacatct cttgcaagtt
     3841 tttcttcctc taccattttt aacaaggcag ttttttcagt ttcatccaca gtttgaaaag
     3901 caaagagacc tacgtaattt ttaagtgtct gatcctgtat caaatctctt tgagaagtat
     3961 atcccttatc tgcttgtctt tttactggta agtttccggg atttttaccc atttgtggac
     4021 cctgagcgaa agaaaatata actgcactta aaactcctgc cattaacaat acttttttca
     4081 tatatctcca cctccggttt agtcaattgt gattatatta taggaggtat ctcttaatat
     4141 tggcttagag agctggtttg agatttaaaa caagcttaaa aaactctggc aaaactgtgg
     4201 cagtaaaaat tatctggatc tgcgtataaa aaactttctt gtttcatcgt accacaggac
     4261 aatggaactt acaccaaagg ctattaaaag atccttaaaa gataaggcac agataccaaa
     4321 aattgatgaa agtgccggta tataaacaac tataagctga agtgccacgg taagcaaaac
     4381 tgcacccaaa agatacttat ttgaccaaag ccctgttttg aaaaccgata cgcttgatga
     4441 tcttaccgaa taagcataga acatctggga tatagtaact atcataaata tcatggtctg
     4501 ccataccggg ctgttgtttg aataatatat gtgtccggtc actattgaag ctgcaccgat
     4561 tattattccg acaatgatta tgtttataag cagtccggtc gaaataactc ctcctttatt
     4621 atttctggga ggtctcttca ttatgtcttt ttctgcaggt tctactccca atgctagtcc
     4681 aggcaaaccg tctgtgacaa ggtttatcca cagaatctga agcggaagca ggggaagcgg
     4741 cattccaata aatggagctg caagcatcag caggatctct gcaaagttgc ttgaaagtat
     4801 atattttata aactttatta tattgtcata tatagctctt ccttcttcaa cggatgagac
     4861 gatggtcgaa aagttgtcat caagcagaac catatcagct gcctgttttg aaacatctgt
     4921 tccggttatt cccatagcta cgccgatatt agattttttt agtgccggtg catcatttac
     4981 accatctccg gtcatggccg cagtatatcc tttgttttgc caagcctgta cgatttttaa
     5041 cttatgttcc ggagaaactc ttgcatagac acttgtagat tccacttgtt tttcaagctc
     5101 ttcaacagtc attttttcaa gctcgcttcc agtaacaacc gtatcgcttt gagttattga
     5161 aagttctttt gcaatatata gggcggtcat cgggtggtca ccggttatca ttacaggttt
     5221 tattccggct gatctgcact tttctacagc agcttttact tcaggtcttg gcgggtcaat
     5281 cattccgaat aaaccaatga gtataaggct atcttcataa actttttccg aagcttcagc
     5341 aggcctgaaa gctgcagcca gaactcttac tcctttacgg gcaagagaat cgttgacagc
     5401 cattaaccta tctctgtcgt tttgagtaat cggtcttatg agtctgccgt ccagtatacg
     5461 gtctgcaaac ttcaaaagac tatcagcagc gcctttggta aaagaaatgt atttaaactc
     5521 tttcaaaata ccataaaggt cttctgagac ttcctttata ttttgaacta aatggagagt
     5581 agtcatcatt tttctgtcag aatcaaaagc cagctcattt atcctgggca ataatgattc
     5641 taatttcctt ttatcgtagc cgtttctctg agctatctca accagtgcac cctcggtcgg
     5701 atcaccgatc atttttccgt tttcttgtat ttcagagtcg ttgcagagta atccggcggt
     5761 caaaagcatt tttatatcag gggaggaaac cttaagtccg tttatatcag ctgctcccaa
     5821 agtacttgta tagattgaag ttacagtcat cttattctgt gtaagagtac cggttttatc
     5881 tgagcagatg actgaaacac ttccaagagt ctcaactgca agtagcttac gtataagggc
     5941 atttctctta agaagccttt gagttccaag ggctagtgaa attgttacca aagccggtaa
     6001 tccttccgga actgctgcaa cagccatgct tatagcggtc ataaaaagaa cttggatatc
     6061 ttctcccctg aaaattccca gcacaaaaac aattgcaaca atagcaagag ctattacagc
     6121 gagcagttta ccaagctgat caagcttttt ctgaagaggc gtgggctgtc tgtcaacgct
     6181 ctgcatcatt tttgcaatat gtccaagctc tgtgttcata cctgtctgag tcactaccgc
     6241 cgttcctctt ccagaagttg aaacagttcc catgtaacct gtattttttc tgtctcctag
     6301 agaaagattt acatcagata tgacatccga cattttttcg ataggttcgg actcccctgt
     6361 aagtgcggac tcctgaactt taagatttac agactgtata attctgcagt cggcaggtat
     6421 tgaatttcct gcatctatct gaataatatc tcccggaaca agctctttgg aagagatctc
     6481 tattaatttt ccttcacgtt tgacctttac cgttggaaga gacatttttc ttagagctgc
     6541 catggctttt tcagctttat actcctgaga aaatccaagc agtatgttta ttaccacaat
     6601 cgccagaatt actatggcat ctgttgtctc accaaggaaa aatgagatta tgcctgcaat
     6661 aacaagcatg attaccataa taccagatgc ctgctccata ataattttcc agggtttttt
     6721 caaagcaccg tcgacgagtt cattttttcc atagctgttc aacctttctt cggcttgttt
     6781 ataagaaagg ccggtggtga tatctgtgtt cagctctttt aaagtttctt caacagtctt
     6841 cctgtaccag tctgccatag tagttttctc cttttttata ataaaaaaga cctctgtcag
     6901 gattatcctg acaaaagtct tgcatatgat tttttgtgca tattacaaaa ccagactttt
     6961 taagtcgtga atgttgattt tgtaatcttc gaataccgaa gatgctactc ccttttgttt
     7021 gtatagaata ttatatacgt aaaaaaagta tttgtcaaat ataaaaagct ccggagagtt
     7081 taatctctcc ggacaagctt ggaatacctg atctttttgg gggtatagat catatattac
     7141 cgcaaattat gttgttttga atttttgggt ttgtgtttca agattttcag aaagggcatt
     7201 aagctcttcg ctatttgcac ttatctgctg tgctccttca ctctgttggt tgacacctgt
     7261 taccatatta tttatctctt cattgatttt aagcattatt ttgcttgcat tctgcatggc
     7321 ggaggaaatt tcctccgagg cagcactttg ttcctgtgcg ctggctgaaa gattttctat
     7381 catggagttt atgtttccta caagagtttc aatctctttg aaattaatgc ttatattttt
     7441 tccaccctca cttacatttt caaccttaga ttttgttaga gaggtggcct gattggcgag
     7501 ctctgttttt ttacgtatat cagtaagaat gtcagaaata tcctgagtgg cttttttgga
     7561 ttcctctgcc agttttctta tttcatcggc tacaacagca aagccttttc ctgcttctcc
     7621 tgctctggct gcttcaattg cagcatttag tgcaagaaga ttggtctgct cggttataga
     7681 gtttatggac tgtaaaatag tttctacgtt acgtgaagac tccgcaagat ctgctacaag
     7741 ctttgatgtg ctttctgtct gacctacggt ttcctgtatg gttgagataa ttccttgtac
     7801 gttcttcata ccgcttgaaa cactttttga aacattgtca gcgtttgacg ccagttcgac
     7861 agaagccttt gaaacgctct gggcactgga tgcaacctcc tctacagaag cattcagttc
     7921 ttctatattg gctgaagcac tctgaacgtt gttttttatc acatcggact gtgctgaaag
     7981 ctcttcactt gtggagcttg attcttgtgc cacagccgca agatcggaag aagaagcgtt
     8041 gagctgtttt gaagcctgaa gaattgattt catggattca tcaagttctt ttgccatagt
     8101 attaaggttt tgtgccatga caactatttc aagacttcct gatggtgtaa acttggttgt
     8161 gagatcacct tttccgaaac tttttatttc gcttagaatc ctttccattg gccgggagac
     8221 attaaagcgt ataaaaaata tggttatgat aagcgcaatt gcagttatgg caacggaaaa
     8281 tacaataagc agtataaggg ttcttttagc ctgttctaaa acctctgccc tatctataag
     8341 agtaaaaaca gtccatccaa gagaggatat cttgttataa catattattt tatcctgatc
     8401 acctatcttc tctcccagaa caccctggtt ttcttttacc tttgaaaaat aagattcttc
     8461 ggatatgctt tttcccatca tactattatc agagtgcagg actattttac ctgcagcatc
     8521 tgcaacaaat gagtaagagc ttctaaaact gttattagcg gatatttctg tgagaaggct
     8581 atctatagcg atatcggctc cggcaactcc tttcaaagcg cctgtgtttc cggtaaaggt
     8641 ctttaccaca gaaaccacca tttttttggt aacagtatca acgtaaggat ctgttgtaac
     8701 cgtagttcca gcatttgcaa cagcctgaat ataccatgaa cgctctttgg gattgtagct
     8761 gctgtctact tcgttatgag gataaaagaa gatactcttg tcttcatatc ctacaaaaaa
     8821 aagctgaatt tccggaaagg tgcttttaag ttttctgaaa tcatcaataa agagtgtaga
     8881 atcatttatg tttttttcat acaaacgcag actgttgttc tgagacatga aatcaacagc
     8941 ttttgagatg ggagctatac tatcttcgat ggtctttgct tttagagaat tggcaacctt
     9001 aagataattt tcagctcctc tgtaatcgct ttgataagaa gatatagaag tcacaacggt
     9061 gagaatgact aagggaagaa ctgaaacgca taagataaga aaaatcataa gcgtttttag
     9121 actctttttc atcttggaat cctccgtaat aatatttccc cccactaaag ttaataaaaa
     9181 gtaaaatata acaataattt attattatat atattataca caagattttt gaccataaaa
     9241 taataaatat aaatattaat atattaaaga ttatacactc ttttacaaaa aaataccatt
     9301 gcatatccta tataaaaaaa cactccaaac attaatgctt gaagtgtttt tgaatattta
     9361 catcaattga ttcagaattt tttcaagttc tttagtcatc ttcagtatgc tttcttcgtt
     9421 aattattaag cagggatcaa accgtaaaac attgtataac ccattagcgc caataagaaa
     9481 tcctttttca aacatcatgg cagaaatatc tgccacaaga attttttggt caaactcaac
     9541 agcatgcatc attcctctgc ctcggacatc ttttatgcac tgaaactttt ctttgagagt
     9601 cataagctct ttagacagaa gctttccttt gttagcggat gccctgataa gatctctttc
     9661 ttcaaactct tttattacag ccgatacaac tgctccgccc aaaggatcgt tctggtggga
     9721 ctgtacatgg cctatgctct ttccggccat ggcagaagct acaatatctg tcatgactat
     9781 ggcgcttacc ggataaccat ttcctatacc ttttcccatg cagactatat ccggcattat
     9841 atcgtaatgc tgaaatccga agtattttcc tgtacgtccg taaccggttg ttacctcgtt
     9901 gcttatgaca tatccccctg agctttttac cctttggctg agttccctta cgagtttctc
     9961 gtgaggaaag agtactctcc cgcttgtcga gcctaattca aatatacaac atgcaacttg
    10021 tgaaaaatca attcctgaaa aaaaactttc aggctcgttt tcatcgtaag cggtaaagtc
    10081 tttttttatt atgcactctt cgtttttata gttgccggtt gttcccagag ccgaataata
    10141 agagttgctg aacataatcg cctttttcct gtctgttgct gcaaatgaga gcttaagacc
    10201 catttctaca gcctcactgc cggaagtaag gaagactgct tttccttttt ttatgcctgt
    10261 tatccttaaa agatcgtcag cagctttttt acttacagag tttaccaatt tggtatgcag
    10321 gtgagttact ttatccagct gatcttttat tgcctggttt actctatcgt tggaatgtcc
    10381 aagcgcacag caccatatgc ctgattcaaa gtcaatgtac tcttttcctg atgagtcata
    10441 catatactca tcctttgctt ttactatgtc ctgttcaaaa tgaggagcag tgtaaaaaat
    10501 attatcagtc atacttttct ccttttcaat ttcaatgtcg taagtatacg tcatggatta
    10561 ctttactcaa tgaatccaca gcttcttcga tctcttcctc attcagcttt gctattgaga
    10621 gtctgaaatg aattttttcc tgtggtttga aaaagaaatc atttccgttg taaggactta
    10681 tgccgtggtc aagaaatatc tggtgaacct gttggatatt tgagtttacg ttattaaaag
    10741 ttatcatcga tgtataaccg cctaaaggct tggtccatga aacatccgga ttttgaatta
    10801 ctttgtccat ctgtgaaaga aaaacagaca ttcttttttt gtaaactctc ttgagttttc
    10861 ttatatgctc atcaaagtat cctctttggc aaaactcaaa ggcggccgcc tgcattatct
    10921 gtccggagga gatatctgag cagtttttaa gaaacgttat cctgtcgata agtggtttat
    10981 ccgcaacaat ccagcctatt ctcataccag ggaaaagaac ttttgaaaag cttccggtat
    11041 agatgactgt tttagaggta tccatggctt ttatcgaaag aggcgatttt ccaaagtact
    11101 tcatctcttc ctcaaagccg tcttctatta taggtactct gagttcctgg cattttttaa
    11161 ggagattttc cctgtcctgc tggctcatgg tgtaacctgt tgggttttgg aagttaggag
    11221 ttgtgtagaa aaaagccggt ttttcacatt tttcaaggaa tgaaaaatcg tatcctcctg
    11281 tttcattaag tgacaactcc tgcacattgc atcggcgcat acgcaaaaaa ttgtaaaggc
    11341 tggcataagt gggagattct gcggcaacgg tgcttccatc ggaaagcagc agcctggtta
    11401 taagatccag tgagttctgt gagccgttgg tgattattat ttcatttata tcagcctgta
    11461 taccgtgttt tttaaggtgt gcagataaaa aggttctaag cggcaggtat ccttttatat
    11521 ccgaatagga aaacattttg gacatatttt cttttaccgc agcattgaaa gctactttaa
    11581 aagtcttgac aggataaagt ctttcatcag gcgatatgga tttcatatca agatactctc
    11641 ttggaaattt aagagttttt tcatagctgt ttgcatctct ggtataaagt tcattaatcc
    11701 tcttatcaat acaggtgttc cagtctattg cagtttttat tgaaggttcg tttttcaatg
    11761 gatatgctct ttttcttcgg acaaaggtat aggagccggg attgctttgt atatagcctg
    11821 taacccagag ttcttcatat gcaagataaa ccgtagagcg gtttactcca agtttttcag
    11881 ccagttcgcg tgtagaggga agtttttccc ccggtctgag atcgccgtta tctataagct
    11941 cacgcatttt ttcaacaatc tgttggtata tcggagttcc aagggttcgg t
//
LOCUS       DAOSHR010000046        11911 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_4528_length_11911_cov_5.369265, whole genome shotgun sequence.
ACCESSION   DAOSHR010000046 DAOSHR010000000
VERSION     DAOSHR010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 11911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11911
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4528_length_11911_cov_5.369265"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="PLU28_03895"
     CDS             complement(<1..432)
                     /locus_tag="PLU28_03895"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HOG34241.1"
                     /translation="MTKKIKIFQTYFIIAFGLFVNSIGWTAFLIPSKVIGGGLTGVGT
                     LIYYITGFPVGISYLVMNFFLIVIAIKILGKKFGVKTIFAICLTSLYTTIFQQIFKTP
                     LVNDSFMATIIGAGLGGAGIGIVFTQGGSTGGTDIIAMIINK"
     gene            complement(425..1372)
                     /gene="fmt"
                     /locus_tag="PLU28_03900"
     CDS             complement(425..1372)
                     /gene="fmt"
                     /locus_tag="PLU28_03900"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOG34242.1"
                     /translation="MKIVFMGTPDFASEVLKELLNKGFNISGVFSQKDKPQGRGKTVQ
                     PTPVKMLCQEKNIKIFQYTSINKGEGFEALKALKPDIIITAAFGQILKLNIVELPPMG
                     CWNIHASILPKYRGAAPIQRAILNGEKETGISIFKIVEKLDAGPVALVKKVEISDKDN
                     FSIVFEKLLKASKEAIVEFLENKDSYCPVEQEGECSYAEKITEKDLLLDFSKRAFEVH
                     NTIRAFDPYPGVRCQLGGQIVKVFSSSLTGIKTSSFPGTVCLTEKESFFVACSDEMIK
                     IGAIQYPGKKIITAKDAINGRKVSIGEIFKKVREDGEND"
     gene            complement(1374..1895)
                     /gene="def"
                     /locus_tag="PLU28_03905"
     CDS             complement(1374..1895)
                     /gene="def"
                     /locus_tag="PLU28_03905"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOG34243.1"
                     /translation="MKVQIRYLGDPILRKKAVEVKVFDNRLQELIDLMYEYMYKYDGV
                     GLAAPQVGLNERFFVIDDGQTKRTVINPHILEYLGEDVEYEEGCLSIPKLYATVMRPE
                     GIKVKYQDVLGNAVEEELHEYTARIFQHEFDHLEGVLFIDKLTVVQRAKIKSELSQII
                     KETKKSLKQLSEE"
     gene            complement(1879..2655)
                     /gene="surE"
                     /locus_tag="PLU28_03910"
     CDS             complement(1879..2655)
                     /gene="surE"
                     /locus_tag="PLU28_03910"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HOG34244.1"
                     /translation="MNILISNDDGIMSPGIAVLKNFLEKDHYVYVSAPDVERSATGHA
                     ITIRNPLWAKEIYIDNKFFGQAINGTPADCVKLGLQAIYRDIEFDVVISGINKGANLG
                     TDILYSGTVSAALEGALSGYPSIAVSCVDFNNPNFETGARCLVDILNKINIDDWPEFT
                     TLNINIPNLRYEEIKGIKVTRQSKRRYNDYFEARKDPFGGTYYWMLGKVIEDDNGDDS
                     DYVAVNEGYVSVTPITVFLTKSDNVGYLKGILEVKDESAD"
     gene            complement(2918..4630)
                     /locus_tag="PLU28_03915"
     CDS             complement(2918..4630)
                     /locus_tag="PLU28_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HOG34245.1"
                     /translation="MTYCVKINDREYKFDEEISILQAAAQAHIKIPTLCYNEKLAPYG
                     SCRLCSVEVEGRKTLMPACVTKIENGMIVKTHSEKVRRVRRTIMELIIASHDISCNLN
                     CLNCSRSSSCEIRQVAQEIGIHSIRIPPIEKKQSVDSSSHAIVREPSKCIVCNRCVRK
                     CAEVQNVNVLTVANRGPKTIITTFMDRGMGNVDCTNCGQCILECPSGALHEAYHLDYV
                     WKAIENSDKMVIVQTAPAVRVAIAEEFGFKPGTIATGQLVAGLRLLGFDKVFDTNFTA
                     DLTIMEEGTEFIKRFKAQKKLPLFTSCSPGWIKFIEHNYPEMLENLSTCKSPQQMFGA
                     VIKSFYAQKIGMDKSKIVVVSLMPCTAKKFEAQRNEMKGDVDYVLTTRELAKMFLQAG
                     IKLMDLSAEDYDSPFGESSGAAELFGSSGGVMEAALRTAYEIITGHELENLDFHEVRG
                     LKGIKEACVKIGTQEVKVAVAHGLGNARILMERIKKGEKFDFVEFMACPGGCIGGGGQ
                     PIPTNEEVIVERMTAVYDIDTLAKHRKSHENESVKRLYKEFFGEPLSERSHELLHTSY
                     TVRH"
     gene            complement(4627..5841)
                     /locus_tag="PLU28_03920"
     CDS             complement(4627..5841)
                     /locus_tag="PLU28_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOG34246.1"
                     /translation="MEKIFLKTDFGKTIDNYEFLGLNKALRMKPSEVLEKVKENCIRG
                     RGGAGFPAGIKWDFVAAEKSEIKFVVCNGDEGEPGTFKDRFLLENLPFKVIEGIIIAG
                     YAVDARYGYIYIRGEYEKAIKSIRESIHKMTERKLLGKSILGSSFGFELKLIRGAGAY
                     VCGDETSLLNSIEGKRGLSRIKPPLPAKEGLYEKPTLVNNVETLSVIAEILKYDQNIY
                     LSLGTPKSRGTKLLSISGDVKNPGVYEIEFGKMSIMQIINDLAGGVKGSGLGFVMPGG
                     IATNIISDEQLDTPYTYEDLEQTGSSLGSGGMIVVSKDRDYLSIVKNISDFFMNETCG
                     TCFPCREGNRNINKMIDKMISDGKVGSQDIEIIREIKQAIGLAARCGFGQASVNSILS
                     ILEKYFEKKVYV"
     gene            complement(5854..6273)
                     /gene="nuoE"
                     /locus_tag="PLU28_03925"
     CDS             complement(5854..6273)
                     /gene="nuoE"
                     /locus_tag="PLU28_03925"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOG34247.1"
                     /translation="MEKFYSKELIEELRDIQDTYGYIPEEQIVKIATKKNIPKAHLYG
                     VISFYSMLYLEPTGKYIIRLCNSVSCRLNNSTEILRSIKEFLKIENGGTTKDKKFTLE
                     VAECLGHCGEGPVMMINGQVYSFLTPEEAIKILKSLQ"
     gene            6513..8663
                     /locus_tag="PLU28_03930"
     CDS             6513..8663
                     /locus_tag="PLU28_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HOG34248.1"
                     /translation="MDILKLISEEMTLKFHQVQNTVTMLEEGDTVPFISRYRKERTGN
                     LNEEEIRKIEELFKYYTNLESYKQTVINSISEQGKLTEELRKEIENALKLSIVEDIYL
                     PYKKRKKTKADMAIQAGLEPLAIMILQGMEVSKSTFDSYLSDDYSTLEKAEEGIMYII
                     GQKFSHDQSIRDSLRNFYAKNGFILSQKKKEFREQKTKYDMYDDFKQEISKIQDYRIL
                     ALDRGEKEDVLSISLSLEEVFIEQLFSNYMTKKMPNDTYIKTGLEYGFKNMLDPSISR
                     EIRQSMSEKAGDGAIELFAKNLRELLMTPPLKNKRVLAIDPGFRTGCKVVALDESGKF
                     LEYNTIFPVPPQNDFEGAEKTVLKMIKSYKLNLIVIGNGTASRETQAFVVNTIKSNSL
                     SLKYIFANEAGASVYSASEIAKEEFPDQDVTVRGAVSIGRRVQDPLAELVKIDPKSIG
                     VGQYQHDVNQKKLKEKLENTVESVVNTVGVNLNTSSYAILSFISGINSSTAKKIVEYR
                     NKNGKFLEREALMNVKGFGQKAFEQSAGFLRIFDGSQPLEVTGIHPESYGAAQLLLKE
                     LGYSASDILDPVKSILLKKALSDCISSDKKLSEYEKKLGVGKFTLSDIIRELQKPGID
                     PRDELTQPILYDDILSFNDLKEGMKLQGKITNITDFGAFVDLGIKESGLIHKSNMGKK
                     FVRHPSEVLSINDIVNIEIITMDKELKRINLRLI"
     gene            complement(8686..9420)
                     /locus_tag="PLU28_03935"
     CDS             complement(8686..9420)
                     /locus_tag="PLU28_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34249.1"
                     /translation="MAQLSRKERFKAIKSLSIILGLMIVIYFVSYFLWGVYEVSLRDM
                     TYYPYIKLKSYDFKQVKNYPYTFKNTSVTIDKTDIPNEYAVSTHNGNTTIRTFDNRYY
                     VYEKDGTIALAYPSYDFRGVEYKLFMQNGKVISFEVVPSENVFKNIEKIMQSFSLDVQ
                     KQKTQDTVEEFISVCCPKGFITDAGESIDIKAKNVRFRVPKDEIISYFMIEEIVYIVC
                     TDDNFKNRMYVYSSDTNLVQEILRIN"
     gene            complement(9439..11163)
                     /locus_tag="PLU28_03940"
     CDS             complement(9439..11163)
                     /locus_tag="PLU28_03940"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HOG34250.1"
                     /translation="MRVRTAVAQLNQHVGDYKKNTDKILGAIEYAVGKKADIILFPEL
                     SLNGYPLEDLILKTQYLKDSQKYLDMISEFSKGKNIIIIIGAVEWDVESYNSAKIIYD
                     GKVWASYKKMFLPNYSVFDEKRYFTPGNMPFMIETGGIKIGVTICEDLWVPNGPSLAL
                     SQNGANIILNLSASPFHKTKHVARRELVKTRASELSSWIVYANMIGGQDEIVFDGASI
                     IADPYGDVVMQAPVFEEGVFVFDIDPYEATRANLREGKRKHYNLSRYTEIRSINIPHS
                     FDNKEEYKNFYSNIPQEYEQLYLAIKTGIRDYIFKNGFKKAVLGLSGGIDSALVAAIA
                     CDAIGSENVLGIMMPSRYSSESSVKDSIILADNLGMKTVNIPIEEPFKAFNELLEPYF
                     EGRKTDSTEENIQARIRGIINMAFSNKFGFIVLSTGNKSEVATGYATLYGDMAGGLSP
                     IKDLYKTEVYKVCYAYNKIHSKDIIPSNIFDKAPSAELRPNQTDQDTLPEYNLLDQIL
                     FRYIEREMSLDEIVEDGFNEEIVKFVIKLVDINEYKRRQGAPGIKLTERSFGKERRMP
                     ITNGYKIF"
     gene            complement(11160..>11911)
                     /locus_tag="PLU28_03945"
     CDS             complement(11160..>11911)
                     /locus_tag="PLU28_03945"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatidylserine/phosphatidylglycerophosphate/
                     cardiolipin synthase family protein"
                     /protein_id="HOG34251.1"
                     /translation="DFFISMLGDKKTDFFIEASLFESEVPKNVRIFRDNNKEGYLHEK
                     FIIFDGKAVLFGTGNFTENSLKNDFNVFVYTQNPIVTALFLEEINNFQNNRYGHDKSI
                     IDRQVKADGCTFRMITGPSVNIYKSLIKEIQNSSQSIDIFSFSFTDPFMVYELEKASA
                     SGVKIRMVSDDWNLMYSSPIQYLKGIEVVYDPGVHAKIMVLDNKKLVIGSYNLTYRAR
                     EKNDEFLVIVDDSKLVSAVREKFELYFKEEK"
BASE COUNT         3599 a         2433 c         1866 g         4013 t
ORIGIN      
        1 cttgtttatt atcatggcaa taatatctgt tccgcccgta cttcctccct gagtaaaaac
       61 tatacctatc cccgcaccac ctagtcctgc tcctattata gtagccataa aagaatcatt
      121 aaccagcgga gttttgaata tctgctgaaa aatcgtcgtg tataaagaag taagacatat
      181 ggcaaatata gttttaacac caaatttttt tcccagtatc ttaatggcaa ttacaataag
      241 aaagaaattc atgacaaggt aacttattcc aacaggaaaa cctgtaatat aatatattaa
      301 agttccgaca ccagtaagtc ctcctccgat taccttggac ggaataagaa aggctgtcca
      361 gcctatagaa tttacaaata atccaaaggc tataataaaa taagtttgaa aaatctttat
      421 ctttttagtc attttctcca tcttctctca ctttcttaaa aatttctcct attgacactt
      481 ttcgtccgtt gatagcatct tttgctgtaa taatcttttt accgggatac tgtattgcac
      541 ctatctttat catctcatcg gaacaagcta caaaaaagct ttctttctct gttaaacaaa
      601 ctgttccggg aaaagaggaa gttttaattc ccgtcagcga cgaagaaaag actttgacta
      661 tctgtccgcc tagctggcat ctaacacccg gataaggatc aaaggctctt atcgtattgt
      721 gaacttcaaa agctctttta ctaaaatcaa gtagcagatc tttttcagta attttttccg
      781 cataagaaca ctctccctcc tgctcgactg gacagtaaga atccttattt tcaagaaact
      841 ctacaatagc ctccttcgac gccttaagaa gtttttcaaa aacaatggaa aaattatctt
      901 tatctgatat ctcaactttc tttaccagtg ctacaggtcc tgcatctaat ttttcaacaa
      961 ttttgaatat gctgattccg gtttcttttt caccatttag tatagcacgc tgtattggtg
     1021 ctgcccctct gtacttcgga agaatagacg catgaatatt ccagcagccc ataggtggaa
     1081 gttctacaat gtttagtttg agtatctgtc caaatgcagc tgtaataata atatcaggtt
     1141 ttaaagcctt gagagcttca aagccttcgc ctttatttat actcgtgtac tggaagattt
     1201 ttatgttttt ctcctgacaa agcatcttca caggagtagg ttgaactgtt ttccctcttc
     1261 cctgaggttt atccttctgc gaaaaaactc cggaaatatt aaaaccttta tttaaaagtt
     1321 cctttagcac ttctgatgca aaatcaggag tacccatgaa aacgatcttc atgtcattcc
     1381 tcagacagct gtttaagact ctttttggtt tctttgataa tctgtgaaag ctcacttttg
     1441 atttttgctc tctgtactac agtcaattta tcaataaaca gaactccttc aagatgatca
     1501 aactcatgct gaaagattct tgcggtatac tcatgaagtt cttcctcaac tgcatttcct
     1561 aaaacatcct gatacttaac ttttattcct tcaggcctca taacagtagc ataaagcttt
     1621 ggaatactta agcaaccttc ttcatactca acatcttctc ccagatactc taatatatgt
     1681 gggttaatta ccgttcgttt ggtttgtcca tcatcaataa caaaaaacct ttcattcagt
     1741 cctacctgag gagctgcaag accaactccg tcatatttat acatatactc atacattaaa
     1801 tctatcagtt cctgtagcct gttatcaaaa acctttactt caactgcttt ttttcttaga
     1861 atgggatcac ctaaatacct aatctgcact ttcatctttc acctcaagaa tacccttaag
     1921 atatcctaca ttatctgatt ttgtgagaaa aacagttatc ggggtgacag atacgtaacc
     1981 ttcatttacc gcaacatagt ctgagtcatc tccgttatca tcttcaatta cctttccaag
     2041 catccagtaa taggtacctc caaaaggatc ttttctagct tcaaaataat cattgtatct
     2101 tcttttgctc tgtcttgtaa ccttaattcc cttgatctct tcgtaccgaa gattcggaat
     2161 atttatgtta agcgtggtaa attctggcca atcgtcaatg ttgattttat ttagtatgtc
     2221 aacaagacat ctcgcaccgg tttcaaaatt cggattgtta aaatcaacac aagatactgc
     2281 aattgaagga tatccgctca aagctccttc aagagccgca gataccgtac cggaataaag
     2341 aatatccgtg cccagattgg ctcctttatt tataccgctt atgaccacat caaattcaat
     2401 atctctgtat atagcctgaa gaccaagttt gacacaatca gccggagtac cgtttatagc
     2461 ctgtccaaaa aatttattgt ctatgtatat ttcttttgcc cataatggat ttcttatggt
     2521 tatcgcatga cctgtagcac ttctctcgac atctggtgca gaaacataaa cataatgatc
     2581 cttttcaaga aaatttttaa gaactgctat tccgggagac attataccgt cgtcattgga
     2641 aataagaata ttcaattgac acctccacaa ttttattatt tttatgattt tatcattatt
     2701 tttttagttg tactttaaga aaagattaaa gtattaacag acacttcatt tttctccgaa
     2761 cagatcaaag ctttactagc cctacaataa acaactataa agcaaaatag ctaaatttat
     2821 ataaaagtac cattacaata cggtattaag ctataagaaa ccggtgctac aataaaacag
     2881 caccggtcta aaaatcacta taaaaacatt tttcttttta atgtcttacg gtataagaag
     2941 tatgaagcag ttcatgtgat ctttcactga gaggttctcc aaaaaattct ttatataatc
     3001 tttttacgga ttcgttctca tgtgattttc tgtgcttagc aagcgtgtct atatcgtata
     3061 ctgcagtcat cctttctact ataacttctt cgtttgtggg aattggctga cctccaccgc
     3121 ctatgcatcc tcccggacag gccataaatt caacaaaatc aaatttttct ccttttttaa
     3181 ttctttccat aagaattctg gcatttccca gaccatgtgc tacagctact ttcacttcct
     3241 gggttccaat tttcacacag gcctctttta ttcccttaag tcctcgtact tcatgaaaat
     3301 caagattttc aagctcatga cctgttatta tttcatatgc tgttctgaga gcggcttcca
     3361 ttactccgcc acttgaccca aacagttctg ctgcaccaga agactctcca aaaggactgt
     3421 cataatcttc agcagaaaga tccataagct ttataccggc ttgtaaaaac atcttggcaa
     3481 gttctcttgt agtcagaacg taatctacat cacctttcat ctcatttctc tgagcctcaa
     3541 atttttttgc agtacaaggc ataagtgaaa ccacaacaat tttactttta tccataccga
     3601 tcttctgtgc atagaatgat tttatcaccg caccgaacat ctgttgtggt gatttgcatg
     3661 tggaaaggtt ttcaagcatt tcaggataat tatgttctat aaattttatc cagccaggac
     3721 tgcaggaagt aaacaatgga agctttttct gggctttaaa tctttttatg aattctgttc
     3781 cttcttccat tatagtgaga tctgctgtaa aattggtatc aaaaacctta tcaaatccca
     3841 aaagacgtaa tccagcaact aactgtccgg tagctattgt tccgggtttg aacccaaact
     3901 cttctgctat agctaccctg actgccggag ctgtctgcac aataaccatc ttatcactat
     3961 tttcaatggc tttccaaaca taatcaagat gatacgcttc atgaagggct ccgctgggac
     4021 attcaagtat acactgtccg cagttggtac agtcgacatt tcccattccc ctatccataa
     4081 aagttgtgat aattgtcttg ggacccctat ttgctaccgt aagaacgttt acattctgca
     4141 cctctgcaca ctttctaaca catctgttac acacaataca ttttgaaggt tcacgtacta
     4201 ttgcgtgact gctactgtca acagattgtt tcttttctat aggaggaatc cttatagaat
     4261 gtatgcctat ctcctgagca acttgtctga tttcacagga ggatgacctg gaacagttca
     4321 agcagttgag attacaactt atatcgtgag acgctattat aagctccatt atagttctac
     4381 gtactctgcg aactttttca gagtgggttt taactatcat tccgttttct attttggtaa
     4441 cgcatgccgg catcagtgtt tttcttccct caacctccac agagcaaagt ctgcagcttc
     4501 cataaggtgc cagtttttca ttgtaacata aagtaggaat ttttatgtga gcttgagcag
     4561 ccgcctgaag tattgaaatt tcttcgtcaa acttatactc tctgtcattt attttcacac
     4621 agtatgtcat acatacacct tcttttcaaa atatttttcc agaattgaaa gtattgagtt
     4681 cactgatgcc tgtccgaaac cgcaacgtgc agcaagacct atagcttgtt ttatttccct
     4741 tattatctca atgtcctgag aaccaacttt accgtctgaa atcattttat ctatcatctt
     4801 gtttatattt ctgtttccct cacggcatgg aaagcaggta ccgcatgttt cattcataaa
     4861 gaaatcagaa atatttttta ctatagaaag ataatctcta tcttttgaaa caacaatcat
     4921 tcctcccgaa ccaagtgaac tgcctgtctg ttcaagatct tcgtatgtat acggtgtatc
     4981 cagctgttca tcgcttatta tgttggtggc aattccgcct ggcataacaa atcccagacc
     5041 gcttcccttt actccacctg ctaaatcatt tataatctgc attatactca tctttccaaa
     5101 ctctatttca tagacacctg gattcttaac atctccgctt atcgacaaaa gtttggtacc
     5161 tctgctctta ggagtaccca agctaagata tatattttga tcatacttaa gaatttcagc
     5221 tatcactgaa agagtttcaa cattattaac aagagtcggt ttttcataaa gtccttcttt
     5281 tgccggtaga ggcggcttta ttctcgacag gccacgctta ccttcaattg agttaagaag
     5341 ggacgtttca tcaccacata cataggctcc tgctccccta ataagtttca gctcaaaacc
     5401 aaaactgctt cccaaaatgc ttttcccaag aagttttctt tctgtcatct tatgaatgct
     5461 ctctcttatt gattttatag ctttttcata ctctcccctt atatatatat acccatacct
     5521 agcatcaacc gcatagccgg caattataat gccctcaata accttgaacg gcagattttc
     5581 caatagaaat ctgtctttaa atgttcccgg ttccccttcg tctccgttac aaactacaaa
     5641 ttttatttcc gatttttcag cggcaacaaa atcccatttt attcccgcag gaaatccagc
     5701 tcctcctctt cctctaatac aattttcctt tactttttca agaacttctg acggtttcat
     5761 tctgagcgct ttatttaacc caagaaattc gtagttatct atggtttttc caaaatcggt
     5821 ctttaaaaat attttttcca tttttaacat cccctattgt aaagacttca atatttttat
     5881 ggcttcttca ggagtcaaga aactgtaaac ctgtccatta atcatcataa ccggtccttc
     5941 gccgcaatgt ccgaggcatt cggccacttc aagagtaaat tttttatctt tggtggtacc
     6001 gccgttttca atcttcaaaa attctttaat acttcttagt atttcggtac tgttgtttaa
     6061 cctgcatgaa acgctgttgc atagccttat tatatactta cctgttggct caagataaag
     6121 cattgaatag aagcttatta ctccatagag atgagcttta ggaatatttt tctttgtagc
     6181 aattttgact atctgctctt cagggatata tccataagta tcctgaatat cccttaattc
     6241 ttctataagt tctttggaat aaaacttctc catatcttca ccccgcaata ttctccaatg
     6301 agattttttt cacaaaagct taattataaa tttcactttc aagtaaatat taactctgtt
     6361 atgtttctta aaaactactt taaagttaca tctttttgat gaagaaacct tttattaatg
     6421 ttatgactga ttttttaaaa tatatctcca cgcttctttt aaactagtaa agtggtataa
     6481 tagaaaaaga tataatgcag gaggtaataa aaatggatat tctaaaactt atttcagagg
     6541 aaatgaccct aaaatttcat caggttcaga acacagtcac aatgcttgag gaaggcgata
     6601 ccgttccttt cataagcaga tacagaaaag aacgcacagg aaatcttaat gaagaggaaa
     6661 taagaaaaat tgaagagctc tttaaatact atacgaatct tgaaagttat aagcagactg
     6721 ttataaacag tataagtgag cagggaaagc tcaccgaaga attacgcaaa gaaatagaaa
     6781 acgctcttaa gctgagtata gtagaagata tttatcttcc ctacaaaaag agaaagaaaa
     6841 caaaggctga catggccatt caggctggtc ttgaacctct tgctattatg attcttcagg
     6901 gaatggaggt atctaaaagt acttttgatt cttatctttc tgacgattat tctactctgg
     6961 aaaaggcaga ggaaggtata atgtacatta tcggacagaa attttcacat gatcaatcta
     7021 tcagagattc tctacgtaat ttttacgcaa agaacggctt tatactttca cagaagaaaa
     7081 aggagtttag agagcaaaaa acaaagtatg atatgtatga tgatttcaaa caggaaatat
     7141 ccaagataca ggattaccgt atacttgctc ttgacagggg agaaaaggaa gatgtccttt
     7201 ctatttcttt atctcttgag gaggttttta ttgaacagct cttttcaaac tatatgacaa
     7261 aaaaaatgcc aaatgacact tatattaaga ccgggcttga atatggtttt aaaaacatgt
     7321 tggatccttc gatcagtcgt gaaatcagac agagcatgag tgaaaaagcc ggtgatggtg
     7381 ctatagaatt atttgcaaaa aatctcagag aactgcttat gacacctcct ctcaaaaaca
     7441 aacgtgtttt ggctattgac ccgggtttta gaactgggtg caaggttgtt gcgctagatg
     7501 agtcaggaaa gtttttggag tataatacta tattccccgt tcctccacag aacgattttg
     7561 aaggagcaga aaaaactgtt ttaaaaatga ttaaatccta taaacttaat cttatagtca
     7621 ttgggaacgg aacagcaagc agagaaactc aggcttttgt tgtcaacact attaagtcca
     7681 actcccttag tttaaagtat atttttgcta atgaagccgg agcttctgtt tattcagcat
     7741 ctgaaatagc caaggaggag tttccggatc aggatgtcac tgtaagaggc gccgtcagca
     7801 tcggaagaag agttcaagat cctctggctg aacttgtaaa gatagaccca aaatccatag
     7861 gcgttgggca gtatcagcat gatgtcaatc agaaaaaact taaagaaaaa cttgaaaata
     7921 ctgtcgagag tgtagtaaat actgtggggg taaatttaaa tacttcttca tacgcaatat
     7981 tgagtttcat atcaggtata aattcctcta cggcaaaaaa gattgttgag tatagaaata
     8041 aaaacggtaa gtttttggaa agagaagctt tgatgaatgt taaaggcttt ggtcagaaag
     8101 ctttcgagca atctgcaggt tttctgagaa tttttgatgg ttcacagcct cttgaagtta
     8161 cgggaattca tccagaaagc tatggagcag cccaattgct tttgaaggag cttggctatt
     8221 cagcctctga tattcttgat cccgttaagt ccatactcct taaaaaagct ctttcagact
     8281 gtatttcaag cgacaaaaag ctttccgaat atgaaaaaaa attgggagta gggaaattca
     8341 ccctctcgga tataatcaga gaacttcaaa agccaggtat tgatccaaga gatgagttga
     8401 ctcagcctat actatatgat gatattctat ccttcaatga tcttaaagag ggaatgaagc
     8461 ttcaggggaa aattactaac atcacagact ttggagcttt tgtagatctc ggaataaaag
     8521 aaagtgggct aatccataaa tctaatatgg gtaaaaaatt tgtccgtcat ccttccgaag
     8581 tccttagcat aaacgatatt gtaaatattg agataataac tatggacaaa gaactcaaac
     8641 gtataaattt aagacttata taataaacta ggacggtttt ccgtcctagt ttattctgag
     8701 tatctcctga accagattgg tatcagatga gtaaacatac atcctgtttt taaaattatc
     8761 atcagtacag actatgtaaa caatctcttc tatcataaaa taagagatta tttcatcctt
     8821 aggaactctg aacctaacat tcttagcctt tatatctatg ctttcgcctg catcagtaat
     8881 aaaacccttt gggcagcata cagatataaa ctcttctacg gtatcctgtg ttttttgttt
     8941 ttgaacatcc agagaaaagc tttgcataat cttttctata tttttaaaca cattctcaga
     9001 tggcacaacc tcaaatgaaa tcaccttacc gttctgcatg aaaagcttgt attcaactcc
     9061 tctgaaatca tatgaaggat atgcaagcgc tatggttccg tctttctcgt atacatagta
     9121 cctgttatca aatgttctta ttgttgtgtt gccgttatgt gtagaaactg catactcatt
     9181 tgggatatca gttttatcga ttgtgactga agtattttta aaggtatacg gataattttt
     9241 cacctgtttg aagtcatatg atttaagctt tatgtacgga taataagtca tatctctaag
     9301 actcacttca taaactcccc acagaaagta ggacacgaag tatatcacaa tcatgagacc
     9361 caatattatc gaaaggcttt ttattgcttt gaatctctct ttcctagaaa gctgggccat
     9421 cttgaaacac ctctattgtt agaatatttt atatccgttg gttataggca ttcttctttc
     9481 ttttccgaaa cttctttctg taagttttat tccgggtgct ccctgtcttc tcttgtattc
     9541 atttatgtca accagtttta ttacaaattt tactatttcc tcattaaatc cgtcttcaac
     9601 tatttcatca aggctcattt ccctttctat atacctgaaa agtatctgat caagcaaatt
     9661 atattctggt aacgtatctt gatcggtctg attcggtctt aactctgcag aaggagcttt
     9721 atcgaaaatg tttgacggta ttatatcttt gctgtgaatt ttattatagg cataacatac
     9781 tttgtatacc tcagttttat aaagatcttt tatcggcgaa agacctcctg ccatatcgcc
     9841 ataaagagta gcatagcctg ttgcaacttc acttttatta ccggtagaaa gtacaataaa
     9901 gccaaattta ttcgaaaaag ccatattaat tatgcctctt atccttgcct gaatattttc
     9961 ttctgttgaa tctgttttcc ttccttcaaa ataaggctcc aacagttcat taaacgcttt
    10021 aaaaggttcc tctataggaa tatttacagt tttcattcca agattatccg caagaatgat
    10081 ggaatcttta acacttgatt ctgatgaata tctggatggc atcattatac caagtacatt
    10141 ttcactgcca attgcatcac aggcaatggc ggccactaaa gctgaatcta ttcctccact
    10201 caatcctaaa actgcttttt taaatccgtt tttgaaaata tagtctctta ttccggtttt
    10261 tatagccaga taaagctgct catactcctg gggtatattg gaatagaaat tcttatactc
    10321 ctctttgtta tcaaaactgt gaggaatatt gatgctcctg atttcggtat agcggctcaa
    10381 attatagtgt tttctctttc cttccctaag attggcacgg gtggcttcat aagggtcaat
    10441 gtcaaaaaca aaaactcctt cttcaaagac aggcgcttgc attactacat ctccatatgg
    10501 gtcggcaatt atgcttgcac catcaaatac tatctcatct tgtcctccaa tcatattggc
    10561 atagactatc cagcttgaaa gttcactcgc tctggtttta accaattctc ttcttgctac
    10621 atgtttcgtc ttatgaaacg gagaagccga aagatttagt attatattcg caccattttg
    10681 tgaaagagca agcgatggtc cgtttggaac ccaaagatcc tcacatattg ttacacctat
    10741 ctttattcct ccggtttcta tcataaaagg catattacca ggagtaaagt atcttttttc
    10801 atcaaaaaca gagtagttag gaagaaacat cttcttgtaa gaggcccata ctttgccgtc
    10861 atatattatc ttggcactgt tgtaagattc tacatcccat tccactgctc cgattatgat
    10921 tatgatattt tttcccttag aaaattcgct gatcatatcc agatattttt gtgaatcttt
    10981 taagtactgt gttttcaata taagatcttc aagcggatat ccgttaagag ataactccgg
    11041 gaaaagtatt atatcagctt tttttcccac tgcatactct atggcgccta gtatcttgtc
    11101 agtgtttttc ttgtaatccc ccacatgctg gttaagctga gcaacagctg ttcttactct
    11161 catttttcct ccttaaaata taattcaaac ttttctctga cagcacttac aagttttgaa
    11221 tcatccacta taactaaaaa ctcatcattt ttttctcttg ctctatatgt cagattataa
    11281 cttcctatta ccagcttttt attgtctaga accattattt tggcgtgtac accaggatca
    11341 tatacaactt ctatgccttt aagatattgg atgggtgagc tgtacatcaa attccagtca
    11401 tcagatacca tccttatttt tactcctgac gcagatgctt tttccagctc atataccata
    11461 aaaggatctg tgaatgaaaa tgaaaatata tcaatgcttt gagaagaatt ttgtatttct
    11521 tttattaagg atttatatat gttaaccgaa ggtcctgtta tcattctgaa agtacagccg
    11581 tctgctttga cttgtctgtc aattatactt ttatcatgac catatctgtt gttttgaaaa
    11641 ttatttattt cttcaaggaa aagcgctgta actataggat tttgcgtata aacaaaaaca
    11701 ttgaaatcat ttttaaggct attttcagta aagtttcccg ttccaaaaag aacagccttc
    11761 ccgtcaaaga taataaactt ttcatgaaga tatccttctt tattattatc cctgaatatt
    11821 cttacgttct taggcacttc gctttcaaaa agggaagctt ctataaaaaa atctgttttt
    11881 ttatctccta gcatagaaat aaaaaaatca t
//
LOCUS       DAOSHR010000047        11793 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_4592_length_11793_cov_6.639802, whole genome shotgun sequence.
ACCESSION   DAOSHR010000047 DAOSHR010000000
VERSION     DAOSHR010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 11793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11793
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4592_length_11793_cov_6.639802"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="PLU28_03950"
     CDS             complement(<1..190)
                     /locus_tag="PLU28_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009462054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjdF family protein"
                     /protein_id="HOG34252.1"
                     /translation="MEKGIRLTVFFEDPFWVGVFEKVEDHQLYVCKYVFGAEPKEYQV
                     YELILTKLNSLYFSRGIFT"
     gene            complement(384..1361)
                     /locus_tag="PLU28_03955"
     CDS             complement(384..1361)
                     /locus_tag="PLU28_03955"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HOG34253.1"
                     /translation="MLKLKYLFDNPDLAQMISKNWDCLSTPVFFRISSNVIYTVKKDS
                     KTFFLRFSPQAEKSITSVIAEVEFLMYLSERGFNVCNPVPSKNLKLVEDVYTPWGSYT
                     AVLFPCSRGVQLSKVKLDNYSAELMGKTLGKLHKLSCQYVPKKFKRKNHDAILDEVSE
                     LAQKYGLEDVLTSAVIIRDELDTLPKDSKNYGLIHYDFETDNLFIDSENQNISVIDFD
                     DSHYNWYVLDIERALNSILEQSGDKEFNDLKSSFIAGYSSERSIDERILEKLPLLTAY
                     ANLYGYTRIYDSVFEKWNNEPQWIGELRTHINSLLDERKKVFLEYTAKK"
     gene            complement(1438..2793)
                     /locus_tag="PLU28_03960"
     CDS             complement(1438..2793)
                     /locus_tag="PLU28_03960"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOG34254.1"
                     /translation="MSEIDELIQKSRKAQKEFERFSQEQVDLVVKEIAKTVYDNAELL
                     AKMAVEETRMGVYEHKVSKNKGKSKTIWNDLKNKKSVDIIKIDEEKNIVEIAKPMGVV
                     GSVTPTTNPTVTCMCNAMFALKGRNSIIIAPHPRAKMTSLKTVELINEALKKYNSPEN
                     LIQVIKDPSIQKTEELMKVVDVVVATGGMGVVKAAYSSGKPAYGVGAGNVQVILDNDI
                     DYETAAEKVISGRAFDNGIICSGEQSIIVDEHDYEKVVKAFESKGAFFVRDQKTKEHV
                     RSMLFINGQINKDVVGQSVKKIAEIAGLNIPENTKVIIVEADGIGKEDVLCKEKMCPV
                     MVSFKYKTFDQALKIAKTNLELEGSGHTAAIHSNNNSNIEKAGICLPISRLVVNAPSS
                     TTAGGSLMNGFAPTTTLGCGSWGNNSISENFTYKHLINISRIGFVNTKIRIPTDEEIW
                     A"
     gene            complement(2809..3924)
                     /locus_tag="PLU28_03965"
     CDS             complement(2809..3924)
                     /locus_tag="PLU28_03965"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyrate dehydrogenase"
                     /protein_id="HOG34255.1"
                     /translation="MELFKLGSKIFKYDTFNDFVHEFKIESTDLIITNQFIYDPIIKE
                     SDAKCNVIFQERYGNGEPSDEMMNGILNEAKKYDFKRIIAIGGGTVIDIAKILVLKDI
                     VDVKDAFERKVPLLKGKTLIAVPTTCGTGTEVTNISIAEIKSKKTKMGLAVPELFPDA
                     AVLIPEFLKNLPYKFYMYSSIDALIHATESYLSPKANTYTKLYAVKAIETILDIYINM
                     KKFGEEYRFERLEDILVASNCAGIAFSNTGVGAVHALSYPIGGNYHVPHGLSNYLFFT
                     EIFKLYVKKNPSGTIKELNRILSGILDCDTFDVYDKLEMLLGKLLQKERLSSYGMKER
                     EVEEFAKSVIANQQRLLANNYVPLSDKEIENIYRILL"
     gene            complement(3939..5231)
                     /locus_tag="PLU28_03970"
     CDS             complement(3939..5231)
                     /locus_tag="PLU28_03970"
                     /inference="COORDINATES: protein motif:HMM:NF014594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HOG34256.1"
                     /translation="MDLKQLYKEKTVSAEEAAFKIPSGSRVVLAHAAGVPTAVINSMT
                     ANKSRYKDVEIVHLLCLNDAPYTSSDCEGHFRHNALFVGGNTRNAVSKNKADYTPCFF
                     YEIPKLFSSGKMPVDVAIINVSRPDKYGYCSYGVSCDYTKPAAENARIVIAEVNDKMP
                     TVMGDNFIHISDIDYIVETSKEPAVLEPAEIGQTEKAIGENCAALIEDGSTLQLGIGA
                     IPDAVLLFLKNKRDLGIHSEMVSDGVLELVEAGVITNKNKTIHKGKMIATFLMGSKRL
                     YDFVDKNPMIELYPVDYVNNPLVIMQNRKMVSINSCLQVDLMGQIASESIGLKQFSGV
                     GGQVDYVRGASMSEDGKSIIAMPSTACDGKVSRIVPFLSQGSAVTTSRNDVHYVVTEY
                     GVADLWGKTLRKRAEALIEIAHPYFRQSLTEEYEKRFS"
     gene            complement(5259..5546)
                     /locus_tag="PLU28_03975"
     CDS             complement(5259..5546)
                     /locus_tag="PLU28_03975"
                     /inference="COORDINATES: protein motif:HMM:NF013286.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HOG34257.1"
                     /translation="MSSPTFDLVKEIIDIKIKPLVQEHGGGIELKSVQGGTVYIKLLG
                     ACRNCPSAQLTVENHIKRILIQHFPNIEKVAVTDNISSDLMDMARKILSHA"
     gene            complement(5557..7023)
                     /locus_tag="PLU28_03980"
     CDS             complement(5557..7023)
                     /locus_tag="PLU28_03980"
                     /inference="COORDINATES: protein motif:HMM:NF023222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase family
                     protein"
                     /protein_id="HOG34258.1"
                     /translation="MALMTPEQYVESLRKLNLKVYLFGELIENPVDHPIIRPSLNSVA
                     KTYELAQMDEYKELMTAKSNLTGETVNRFCHLHQSTEDLKNKVKMQRLLGQKTAACFQ
                     RCVGMDAFNALYSTTYETDEKYGTTYHKNFIEYMKYVQTNDLTVDGAMTDPKGDRGLA
                     PSEQQDPDLYLRIVERRKDGIIVRGAKAHQTGAVNSHEHLIMPTCSMREEDKDYAVAF
                     SVPSDAPGVFMIYGRQSCDTRKLENSSIDVGNSTYGGHEALVVFDDVFVPNDRIFLCG
                     EYDFAGMLVERFAGYHRQSYGGCKVGVGDVLIGAAALAADYNGVPKASHIKDKLIEMI
                     HLNETLYSCGIACSTEGKKTKAGNYQIDLLLANICKQNVTRLPYEIARLAEDIAGGLM
                     VTMPSEKDLNSPEIGKYVEKYLKGSSKVSTKNRMRVLRLIENMTLGTAAVGYRTESMH
                     GAGSPQAQRIMISRQADLEQKKNLAKVIAKVDESENKK"
     gene            complement(7524..8606)
                     /locus_tag="PLU28_03985"
     CDS             complement(7524..8606)
                     /locus_tag="PLU28_03985"
                     /inference="COORDINATES: protein motif:HMM:NF024744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HOG34259.1"
                     /translation="MKKNVLKSITVFIFITLFSTVIFAAGTQGFSGNVAEIDKYGNVR
                     LDVTEEAVKLSGVEVGDILEVKFNDKTLILPFATAYGDVDRSSALLRLNKGFVQMAIN
                     YGNFATFFSVALNNTVTVSVLEKGTYKAQIELRKLVKSEERKDYPSDQAFANFREISL
                     GWIKPGILFRTSHPSVADSRSSYVAAFVEEYQIQTIINLADTQAELQENLKNSKYYED
                     LYNKGSVIALGMGVDLLSKDFAAKLKTAFEFMIEHRGPYAIHCLEGKDRAGIVSAILE
                     GLMGATVDEIVNDYMITYENYYSMVKGTERYDLIAKIMTDQLKEMNSNKPVTDLNLRS
                     VMFLYLKNTVGLSDEQIALLRTRISE"
     gene            8781..9677
                     /locus_tag="PLU28_03990"
     CDS             8781..9677
                     /locus_tag="PLU28_03990"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HOG34260.1"
                     /translation="MKKFWILFMVLTTVLSVFSINISVSIYPYYLAVREIAGQNDNVN
                     LIIPQGKSPHTYALTVENMKNLYESDILIVNGLDVEVFIEKVLVSLQQKKIPVLYVAQ
                     SIKQDELSVEEEEDHDEESEDHKENGHVHSGVNPHVWTSPYLMYNYMIPAITAELSKV
                     NPKSKTLYEANAQKLIQKVKAQDQQFLSSSKELNISFFTFHNSFPYFAQRYGFKIAGV
                     VQLSPGIDPTPSQMKDIIDKAKVENANVIFIEPQLSDRAAKAIAASAGLKIGMLDPLG
                     NSSMKTITDLYQYNFDQIMKLK"
     gene            9686..10069
                     /locus_tag="PLU28_03995"
     CDS             9686..10069
                     /locus_tag="PLU28_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOG34261.1"
                     /translation="MKLTRARREILSIFKNSDEPLSAEKLNGLLGFNYDLSTIYRNLN
                     FFTSENILKTMVFSDKVTYYYYGNGHFHYIYCVKCKSFSRFDMCFEEKLSEYIMQQLE
                     FEILDHTLYFEGICKDCKNRSKKNG"
     gene            10062..10808
                     /locus_tag="PLU28_04000"
     CDS             10062..10808
                     /locus_tag="PLU28_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HOG34262.1"
                     /translation="MDKKTAICVNNLNYKIENHVILQNIDFEIHTGDFVGIIGPNGAG
                     KSTLIKVILNEIKDYTGEIKINGKIGYVPQYDEIDRSFPITVKEVALMGMYKEVGFLR
                     RYTAEHVKKVMEVLKILGIDHLKDRKAGRLSGGEYQRLLLARALVSKPDILILDEPEA
                     GVDKNGQILFYGLLKKLNQQEKVTVILVSHDLSMVFKETTKVMCLNRTLHCHKNTAEM
                     SSQDLKDIYAESLEMLVHIDNPLKVVSRND"
     gene            10801..11610
                     /locus_tag="PLU28_04005"
     CDS             10801..11610
                     /locus_tag="PLU28_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HOG34263.1"
                     /translation="MIEILSYQFMQYALAASLLSGVSCAILSNFIVLKKMEFIGDGAG
                     HTAFGGIALALLMGWNLNLVSIITAIIFASAIFIISKKKSINENSIIGVMLSLSMALG
                     IIFISLKTGYTPEISGYLFGDILMVTFNDIVLLLVVFAIILMSTVFFNKELKYYAFNQ
                     RMAKIYGVPVDFIDYIFLLTVSLVVVTSVKIIGIILVTALLLTPGIIAKMWATTLNQM
                     IVLSCFIGFLGALFGVFISYFMNIPPGPAIVVMLFGFFIVSMMLARLKKTT"
BASE COUNT         3455 a         2274 c         2038 g         4026 t
ORIGIN      
        1 ctgtaaagat acctctgctg aagtaaagag agttaagttt ggttaaaatg agttcataaa
       61 cctgatactc ttttggctct gcaccaaaaa catatttgca cacatatagc tggtgatctt
      121 caactttttc aaatactccg acccaaaaag ggtcttcaaa aaaaactgtt agtcttatgc
      181 ctttttccat acatcctcct aaacaaaaaa ttggagaatg gacatccgtt atacggaagg
      241 ctactaacag tttttactgt gtccggacta ccaaccggac tgtgtttttt tctctttaaa
      301 taagttaagt attaaatttt tagtttataa atcaatcaca atgataacat tttattgtta
      361 aaaaaaattt ccatgatctt aaattatttt ttagcggtat actccaagaa aacttttttc
      421 ctttcgtcta atagactgtt tatgtgagtt ctaagctctc caatccactg tggttcatta
      481 ttccattttt caaatactga atcgtatatt cttgtgtaac cgtaaagatt tgcataagca
      541 gttaaaagcg gtaatttttc aagtatcctt tcatcaatag atctttctga agaataaccg
      601 gcaataaaag aagacttgag atcattgaac tctttatcgc cgctttgttc aagaatactg
      661 ttcagagctc tttctatatc aagtacatac cagttataat gactgtcatc aaaatcaata
      721 accgatatat tttggttttc tgaatcgatg aaaagattat ctgtttcaaa atcataatgt
      781 attagaccat aatttttact gtcttttggc aaagtatcaa gttcgtctcg aataatcacg
      841 gcggaggtta aaacgtcttc taaaccatat ttctgtgcga gttctgaaac ctcatctaaa
      901 attgcatcat gattttttct tttgaatttc ttaggaacat actgacaact cagtttgtga
      961 agcttgccca gagtttttcc cataagttca gccgaataat tatccagctt tactttgcta
     1021 agctgtaccc cgcggctaca tgggaaaagt acagctgtgt agctacccca aggggtataa
     1081 acatcctcta ccagtttgag attttttgaa ggtacagggt tacagacatt aaatcctctt
     1141 tcgcttaagt acataagaaa ctcaacctca gcaataactg aggttatact tttttctgct
     1201 tgtggcgaaa atctaaggaa aaaagtttta ctatcttttt tgacagtata tattacattg
     1261 gacgaaattc tgaaaaaaac aggagttgac aggcagtccc agtttttgga aatcatctgg
     1321 gcaagatccg gattatcaaa aagatatttg agttttaaca tttctcagcc tccattcaat
     1381 tgttaaaact attatacacg ataaaaaaag aaaagaccat aaaggtcttt ccttttttca
     1441 ggcccagatt tcttcgtcag tgggaatacg tattttggta tttacaaagc ctatacgtga
     1501 aatgtttatt aaatgcttat aggtaaagtt ctcagatatg gagttgtttc cccatgaacc
     1561 gcatccaaga gttgtggttg gtgcaaaacc gttcattaaa ctacctcctg ctgtagttga
     1621 agaaggtgca tttacaacaa gccgtgaaat tggaagacat atccctgctt tttcgatatt
     1681 tgaattgttg ttggaatgaa tggccgcagt atgtcccgaa ccttcaagtt caagattggt
     1741 ctttgcaatt tttagtgcct gatcaaaggt tttatacttg aagctgacca taaccgggca
     1801 catcttttct ttacacagga cgtcttcttt tcctatacca tcagcttcta ctatgattac
     1861 cttggtattt tccggaatat tcagccctgc tatttcagca atctttttta cagactgtcc
     1921 cacaacatct ttatttatct gtccattaat aaaaagcata cttctgacat gttccttggt
     1981 tttttgatct cttacaaaaa aggcaccttt gctttcaaaa gctttcacta ctttttcata
     2041 gtcatgctca tcaactatta tactctgctc gccggaacag attatgccgt tatcaaaagc
     2101 tcttcctgat atgacttttt ctgctgctgt ctcatagtca atgtcgttgt ccagtataac
     2161 ctggacattt cctgctccga ccccatatgc tggtttaccg ctggaatatg cagccttgac
     2221 aacgcccatt cctcctgtag cgacgacaac atcaacaact ttcataagct cttcagtttt
     2281 ttgaatactc ggatccttta ttacctgaat aagattttcc ggagaattat attttttgag
     2341 tgcttcatta ataagttcaa ctgttttcaa agaagtcatt tttgcccttg gatgaggtgc
     2401 gattattatg gagtttctcc ccttaagcgc aaacattgcg ttacacatgc acgtaacagt
     2461 agggttcgtg gtaggagtca cggaacctac aactcccatg ggtttagcta tttcaacaat
     2521 atttttttct tcatcaattt ttattatatc aacagatttt ttatttttga gatcattcca
     2581 tatggttttt gactttcctt tgtttttaga aaccttgtgc tcataaactc ccattctggt
     2641 ttcttcaaca gccatttttg caagtaactc ggcattatcg taaacagtct ttgcaatttc
     2701 cttcaccact aggtcaacct gttcctgact gaacctttca aactcttttt gtgcttttct
     2761 agacttttga atcagttcat ctatttcaga catattcatt ttcctccgtt ataataatat
     2821 tctgtatatg ttttctatct ctttatcgga aagaggaaca tagttgttag caagaagcct
     2881 ttgctgattg gctataacac tctttgcaaa ctcttcaacc tcgcgttcct tcattccgta
     2941 agaagaaagc ctttctttct gcaaaagttt tcccaaaagc atttcaagtt tatcgtatac
     3001 gtcaaaagta tcacaatcta gaataccaga aagtattctg ttcagttcct ttatcgttcc
     3061 tgaaggattc ttttttacat aaagcttaaa tatctctgta aagaaaagat agttagaaag
     3121 tccgtgaggg acatgataat ttccccctat aggataagaa agtgcatgca cggctcctac
     3181 tccagtattg gaaaaagcta ttcctgcgca gttacttgca accagaatat cttcaagcct
     3241 ttcaaatctg tactcttcgc caaacttttt catattaata tagatatcaa gtatggtttc
     3301 tattgctttc acagcataaa gcttggtata tgtatttgct tttggtgaaa gatatgattc
     3361 ggtcgcatga ataagagcat caatagagct atacatataa aatttataag gaagattttt
     3421 caaaaactca gggataagaa ctgccgcatc tggaaacaat tcaggaactg caagccccat
     3481 cttagttttt tttgatttta tttcggcgat agaaatattt gtaacctcgg ttccggtacc
     3541 gcaggtagta ggaactgcta taagagtttt tccctttagg agaggaactt ttctttcaaa
     3601 agcatccttt acgtcaacaa tatctttgag aacaaggatc ttagcgatat ctataacagt
     3661 acctccgccg atagcaatta ttcttttaaa atcatatttt tttgcttcgt tcagaatacc
     3721 gttcatcatt tcatctgaag gttcaccatt tccgtatctt tcttgaaata ttacattaca
     3781 ctttgcgtct gactccttta taatcggatc atatatgaac tgattggtta ttatgagatc
     3841 agtactttct attttaaatt catgcacaaa gtcattgaat gtgtcgtatt taaagatctt
     3901 gctgcctagc ttgaaaagct ccatgctatc atcccttttt atgaaaatct tttttcgtat
     3961 tcttctgtca gactttgtct gaaatatggg tgagctatct ctattaaggc ttcagcacgt
     4021 tttctgagag tctttcccca tagatcagca actccgtatt cagttacaac ataatgcaca
     4081 tcgtttcttg aagttgtaac ggcacttccc tgtgaaagaa aaggtacaat tcttgatacc
     4141 tttccgtcac atgcagtaga cggcatggca attattgact ttccatcttc tgacatgctt
     4201 gctcctctta cataatccac ctgtccgcct actccgctga actgtttgag tcctatgctc
     4261 tcagatgcga tctgtcccat aaggtctacc tgaagacacg agtttattga gaccatcttt
     4321 ctgttttgca ttatcaccaa tggattattt acataatcaa caggataaag ttcaatcatc
     4381 ggatttttat cgacaaaatc gtaaagtctc ttgctaccca tgagaaaagt tgcaatcatc
     4441 ttacctttat gaatagtttt atttttgttt gttataacgc ctgcctcaac cagttctaat
     4501 actccgtcgg aaaccatttc gctgtgtatg cctagatccc ttttgttttt cagaaaaagc
     4561 agtactgcat caggaatggc tcctattcct aactgtaatg ttgaaccatc ttctataaga
     4621 gcagcgcagt tctcaccgat tgctttttct gtctgtccta tctctgccgg ttccagaaca
     4681 gcaggctctt ttgaggtttc tactatataa tctatatcgc ttatatggat aaagttatct
     4741 cccattactg taggcatttt atcgtttact tcggcaatta ctattcttgc gttttcagca
     4801 gctggtttag tataatcgca agacacccca taactgcaat aaccatactt gtccggtctg
     4861 cttacgttta ttatggcaac atcaacaggc attttgccgc ttgaaaaaag ttttggaatc
     4921 tcataaaaaa agcatggcgt ataatctgct ttgttttttg aaacagcatt tctggtattt
     4981 ccaccaacga aaagtgcatt gtgtctgaaa tgtccttcac agtcgctgct cgtataggga
     5041 gcatcattaa gacataaaag atggactatt tcgacatcct tgtatctgct tttgttggct
     5101 gtcatactgt tgattactgc agttggaact cctgctgcat gcgcaagaac aactcttgaa
     5161 ccggaaggta ttttaaaagc agcttcctca gccgatactg ttttttcctt gtataactgt
     5221 ttaagatcca tactttagcc gctccttatc aaaaagtatt atgcatgaga tagaattttc
     5281 ctagccatat ccataagatc gctgcttatg ttatccgtta cagcaacttt ttctatattg
     5341 ggaaagtgct gtatcagtat tcttttgatg tggttttcaa cggtaagctg ggcggaagga
     5401 cagttcctac aagctcccag tagcttgata taaacggtac cgccctgaac acttttgagt
     5461 tcaattcctc cgccatgctc ttgtactaga ggttttatct taatgtcgat tatttctttt
     5521 actaaatcga aagttgggct gctcataaaa gtcacttcat tttttatttt cggattcgtc
     5581 tacttttgct attactttcg caagattctt cttctgctct agatctgcct gtcttgatat
     5641 catgatcctc tgtgcctgag gtgaacctgc accgtgcatt gattctgtcc tgtaacctac
     5701 tgctgctgtt ccaagtgtca tattttcgat aaggcgaagt actcgcattc tgttttttgt
     5761 agatactttc gagctgcctt taaggtactt ttccacatat tttcctattt caggactgtt
     5821 aagatctttt tcagaaggca ttgtaaccat aagaccgcct gctatgtcct ctgcaagtct
     5881 ggcaatctca tacggcagtc tggtaacgtt ctgcttacag atattggcca gcagaagatc
     5941 tatctggtaa tttccggctt tagttttctt cccttctgtt gaacaggcga taccacaact
     6001 gtaaagtgtt tcattaagat gaatcatctc gatcagttta tcttttatat gacttgcctt
     6061 tgggacaccg ttataatctg ctgcgagtgc cgctgctcct atcagaacgt ctccaactcc
     6121 aaccttacat cctccgtagg actgtctatg gtaaccagcg aatctttcca caagcattcc
     6181 ggcaaaatca tactcaccgc ataagaaaat cctgtcatta ggtacaaaaa catcgtcaaa
     6241 tactaccaat gcctcatgac cgccataagt gctgtttcca acgtcaatag aagaattttc
     6301 aagctttctg gtgtcgcaag attgtcttcc ataaatcata aatacacctg gtgcatcgct
     6361 tggaacagaa aaagccacag cgtagtcttt atcctcttct ctcatagaac atgtaggcat
     6421 tataaggtgt tcatgagaat taaccgcacc tgtctgatga gcttttgctc ctctgacaat
     6481 aataccgtcc ttccttcttt caacaattcg caggtaaaga tccggatcct gctgttctga
     6541 aggtgcaagt cccctgtcgc ctttagggtc tgtcattgct ccatcgacgg tcagatcgtt
     6601 tgtctgtaca tacttcatat actcaataaa atttttatga taagtagtac cgtatttttc
     6661 atcagtttca tatgttgttg agtaaagtgc attaaaagca tccattccaa cgcatctctg
     6721 aaaacaggca gctgttttct gacccaaaag tctttgcatt tttactttat tcttgagatc
     6781 ttcagtgctc tggtgaagat gacagaatct gttgacagtt tcaccggtaa gattggactt
     6841 tgccgtcata agctctttgt actcatccat ctgggccagc tcgtatgttt ttgccacaga
     6901 gtttagcgaa ggtcttatta tcggatgatc tacaggattt tctatcaact ctccgaaaag
     6961 gtaaaccttc agattaagct ttctcaaact ctcaacatac tgctcaggtg tcatcaatgc
     7021 cattagaatc atctcccgtt tttaagttct ttttatcact tggattatac tttttttcaa
     7081 tttttccttg ccacttatac tgtaattttt tttcaggtac ttatagaagc tagaaaatat
     7141 aagttttata aaaatgaagt tattattttc acttcatagt aaaagtataa aagtgtttta
     7201 tttcactttt ttattttcaa cacaattcta ccacttattt ctgaaaaaaa tacactgaaa
     7261 aattatttta tggttaagtt ttacgctggg aattcatagt tttgtttctt aaccctttaa
     7321 ttatttactg atttgttata aaacgcacaa tttaaaaaag acttttttga ttttaaatgc
     7381 atttatttac gaaaatatct taaaattagc ttttcctgac gaaaaaaata attatcacat
     7441 atatattgaa ggatttaaca taataatgtg atttttaata aaaaaactgc ctacgtaatt
     7501 acgcaggcag ctaataagat aagttactct gaaattcttg ttctgagaag tgctatctgt
     7561 tcatctgaaa gtcctacagt gttctttagg tacagaaaca ttacggatct caaattaaga
     7621 tcagttaccg gtttgttgct gttcatctct ttcagctggt cagtcatgat ctttgctatg
     7681 aggtcgtatc tttcggttcc ttttaccatg gagtagtagt tttcataggt tatcatataa
     7741 tcgttaacga tttcatcaac ggttgccccc atgagtcctt caagtattgc tgatactatt
     7801 ccggctctgt ctttgccttc aagacaatgt atggcgtaag gacctctgtg ttctatcata
     7861 aactcaaagg cagtttttaa ttttgccgca aagtcttttg aaagaaggtc tactcccatt
     7921 ccaagagcta ttacagaccc tttattatag agatcttcat agtactttga atttttaagg
     7981 ttttcctgca gttcagcctg tgtgtcagca agattgatta tggtttgtat ctgatattcc
     8041 tctacaaaag cagcaacgta ggacgatctt gaatctgcta ccgatggatg tgatgtcctg
     8101 aaaagaatac cgggttttat ccatcctaaa gaaatttctc tgaaattcgc aaaagcctga
     8161 tctgatggat agtccttgcg ttcctccgat tttacaagtt ttcttaattc aatttgagcc
     8221 ttataagttc ccttttcaag aacagaaacg gttacggtat tgtttagtgc aactgagaaa
     8281 aatgttgcaa agttaccgta gttaatagcc atctgaacaa atcctttatt taatcgtaaa
     8341 agagcggatg atctatcgac atctccgtat gccgtagcaa aaggaaggat aagagtttta
     8401 tcgttaaact tgacttcaag aatgtctcct acctccactc cagaaagctt tacagcttct
     8461 tccgtcacgt caagtcgtac attcccgtac ttgtctatct cggctacatt tccggaaaaa
     8521 ccttgagttc cagcagcgaa aattactgtt gaaaaaagag taataaaaat aaatacagtt
     8581 atggatttca aaacgttttt tttcaaaata atccctcccg aatttatgat atgtaaggat
     8641 attatactac aataagaaaa atttgtcttt gaacataagc tgatttgaaa cttttgcttc
     8701 attttaaagt tactttagtc tgataatata ctttttgcaa atcaattgca tttgcaaatt
     8761 gttttcataa ggaggagaac atgaaaaaat tctggattct ttttatggta ttaacaacag
     8821 tactttcagt tttttccata aacatatccg tatctattta cccatattac cttgctgtca
     8881 gagagattgc aggtcaaaac gataatgtaa atcttatcat accgcagggc aaaagccctc
     8941 acacctatgc tctgaccgtt gagaacatga agaaccttta tgaatcagat attttgattg
     9001 taaacgggct tgatgtggag gttttcattg aaaaagtcct tgtttctttg caacagaaga
     9061 agataccggt tttatatgtg gctcaaagta tcaaacagga tgagctgtct gttgaagaag
     9121 aggaagatca tgatgaagaa agcgaagacc acaaggaaaa tggtcatgtt cattctggag
     9181 taaatcctca tgtttggacc agtccttatt tgatgtataa ttatatgata cccgcaatta
     9241 ctgctgagct ttcaaaggta aatcctaagt ccaaaactct ttatgaagcg aatgcgcaga
     9301 aacttattca gaaagttaaa gcacaggatc agcagtttct ttctagttca aaagaattaa
     9361 acatatcttt ttttacattc cataactctt ttccatactt tgctcaaaga tatggtttta
     9421 aaattgcagg tgtagttcag ctttctcctg gaatagatcc tactccttca cagatgaagg
     9481 atataatcga taaggctaag gtagaaaatg caaatgtgat tttcatagag cctcaactaa
     9541 gcgatagggc tgccaaagca atagcagctt ctgccggact taagatagga atgcttgatc
     9601 ccttgggtaa ctcgtccatg aaaaccataa cagacctgta tcagtataat tttgatcaaa
     9661 ttatgaaatt gaagtaggtg ataaaataaa attaacccga gcaagacgtg aaatactaag
     9721 tatttttaaa aactcagatg agccgttgag tgctgaaaag cttaacggcc tgctgggctt
     9781 caattacgat ctttcaacta tatacaggaa tcttaatttt tttacatccg aaaatattct
     9841 aaaaacaatg gttttttcgg acaaggttac ttattattac tatgggaacg ggcattttca
     9901 ttatatatac tgtgtgaaat gtaaatcttt cagcaggttt gatatgtgtt ttgaagagaa
     9961 gctatcagag tatatcatgc aacagcttga gtttgagatc ctggaccata cgctttattt
    10021 tgagggcata tgtaaagact gcaaaaacag gagcaaaaaa aatggataaa aaaacagcaa
    10081 tctgtgtaaa caatctcaac tataaaattg aaaatcatgt tatcctacaa aatatagact
    10141 ttgaaattca taccggtgat tttgtgggaa taataggacc taatggtgca ggaaagtcta
    10201 cacttataaa agtaatcctt aatgaaataa aagattatac cggagaaata aaaataaacg
    10261 ggaaaatagg atatgttcca caatacgatg aaatagacag aagctttccc ataaccgtta
    10321 aagaagtagc actgatgggt atgtataaag aagtaggttt tcttaggcgc tataccgcag
    10381 agcatgtgaa aaaagttatg gaagtattga aaattttggg aatagatcat ctgaaagaca
    10441 gaaaagcagg aagactttcc ggtggagagt atcagagact cctgcttgca cgtgctcttg
    10501 tatcaaaacc ggatattctt attctggatg aaccggaagc cggggttgat aagaacggac
    10561 agatattatt ttacggtctg ttgaaaaaac ttaaccagca ggaaaaagtg actgtgatac
    10621 ttgtgagtca tgatctttct atggtgttta aagaaaccac caaagttatg tgtcttaaca
    10681 ggacgcttca ctgccataaa aatactgcag agatgtcttc acaggatctt aaagatatat
    10741 atgccgagag ccttgaaatg cttgtgcata tagacaatcc tttgaaagtg gtgagcagga
    10801 atgattgaaa tactaagtta tcagtttatg cagtatgctc ttgcagcatc cttgctttcg
    10861 ggagtaagct gtgcgatact ttccaacttt attgttttga aaaaaatgga gtttataggc
    10921 gatggtgcag gacatacagc tttcggtgga atagccttgg ccttgcttat gggctggaat
    10981 ctgaaccttg tttcgataat aactgctatc atatttgcca gtgccatatt tattataagt
    11041 aaaaaaaaga gtattaatga aaacagtatt ataggagtta tgctttctct ttcgatggct
    11101 ctgggaataa tattcatttc gcttaagacc ggctatacac cagaaatatc cggatacctt
    11161 ttcggggata ttcttatggt tacttttaac gatattgtac ttcttcttgt tgtttttgca
    11221 ataatactta tgagtactgt ttttttcaat aaagagctta aatactatgc attcaatcaa
    11281 agaatggcaa aaatatatgg ggttcctgtg gactttatag actatatatt tttgcttaca
    11341 gtatctcttg ttgtggttac ttcggtaaaa attataggga ttatactcgt aacggcgctt
    11401 ttacttactc ctggcataat tgccaagatg tgggccacga cgcttaatca gatgatagta
    11461 ttatcttgtt tcataggatt tttgggagca ttattcggtg tttttatttc atactttatg
    11521 aatattcctc ccggaccggc aatagtagta atgctttttg gattttttat agtatcaatg
    11581 atgcttgcca gactaaaaaa aacgacataa aacagtaatt ttaaagtacc ctgtggctgg
    11641 aacaattcaa tccacagggt actttttgat atctatctgt tttttataat ggttttgagt
    11701 acttgtccag taaaactttt ttcacattcc aaaatctttg aaactgttcc ctgaaaaatg
    11761 aggaatcctc cgttgtctcc gccttccggt cct
//
LOCUS       DAOSHR010000048        11773 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_4597_length_11773_cov_5.593617, whole genome shotgun sequence.
ACCESSION   DAOSHR010000048 DAOSHR010000000
VERSION     DAOSHR010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 11773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11773
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4597_length_11773_cov_5.593617"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="PLU28_04010"
     CDS             complement(<1..284)
                     /locus_tag="PLU28_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34264.1"
                     /translation="MLVILFCTVLTGQDYFEIVERFDIGPRAFHIDTHKNYATVCDYD
                     GKVTVFDIYTLESFSYSSCVRPMAGFFNGEYLYVVDNYRKEVLKLRGNTV"
     gene            492..1118
                     /gene="udk"
                     /locus_tag="PLU28_04015"
     CDS             492..1118
                     /gene="udk"
                     /locus_tag="PLU28_04015"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF004018.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HOG34265.1"
                     /translation="MVLVAIVGGSGAGKTSVAKKIAEHFADRANVLSMDDYYRDLPPG
                     MTLKEFNFDDPKAFDFDYFIKNTKKLKDGSPINIPVYNMVTCRREVGISTSFFPNDLV
                     IIEGILVLHEPRLRELFDFSVYIDAPADERLIRRIERDTIERGRSIESILTQYRTFVA
                     PSFRAFIEPQKYTCDIILPDGVSNITGMNIIINAIEKMLEINRQEEGK"
     gene            1115..2806
                     /locus_tag="PLU28_04020"
     CDS             1115..2806
                     /locus_tag="PLU28_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34266.1"
                     /translation="MKKSIIFMILFILLVFTVCFAMDDARVKSEYISYIQKYEMFLAD
                     SSQESELKSFFDEIKDIGLYRFYRNIMVGTADYTDVPQGVQRYLSDTSSSQVFESIEQ
                     KLAFAGLLCYVQTDLSGQTITSEIIRSQRVFNDATSEYLRSVDDERFTYFGNVIAYSL
                     GLIESSPYTDIKKFSFSGKLESPEYFTFTGEPIFDYAKIIKEEKQRIEESIRKISESG
                     LTGSDLEFEIDRASDAISSHAVEKAKSDIDSVITILIESAVRGRDYFLLRYIVYVAAI
                     IIFLIFFKKIFKYLLAVIFTSEFLVVLFTFNFSEDVISSFIYGSFIIVAVVIYFVFML
                     FRSFGKGVSVFSRMVNIIIFVCLVLLFFTPYVYVRDLGMVRNIDFCDSKYSTYLLNDT
                     VLSKNSLLFKAIQKGDYDSAKRQISRIVSYGSPQYKEFIKPHIQKIISDYNVGDKFAN
                     FERDFELMKSSENIAVFAHNTQYGTKVLVLFFLSLFLFMLSEKGILKKLSIAAIAASV
                     VLSFVSPSKMIILSEVRYPMLTSTEYSLNFVFNLIMVGMSILLFLNSKSRGGKKV"
     gene            2803..3873
                     /locus_tag="PLU28_04025"
     CDS             2803..3873
                     /locus_tag="PLU28_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOG34267.1"
                     /translation="MKKLLLFVMIVTMLVSSAFSFKAIMVTDTGGLGDKSFNDGTWSG
                     VLRAKNELKIEAEVIVSKEQTDYIPNLTDAAKRSDVVLAVGFLMADALAKIAPQFPDK
                     KFIGIDIVIDPIPSNVALYNFKEQESAFLVGYIAGSVNKTGKVGFLGGIKVPAVARFE
                     IGYRAGVEAYNQLKGKNTEVLIAYANHFGDAPKGKSLSLAQMEQGADIVFAAAGATGN
                     GAFEAAKEKALALYGLKDGASLDAIKNAYKKIGKGYFAIGVDVDQDYLAPGIILASAM
                     KGIDVAAFEGIKSAMGRKFASGNNVVGLKENGVRLSALTYTKDMIDPTALKEIEKLIE
                     DIKSGKLIIPEDEVGLKNFKVK"
     gene            3984..5561
                     /locus_tag="PLU28_04030"
     CDS             3984..5561
                     /locus_tag="PLU28_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG34268.1"
                     /translation="MSSTAEINYENYAVVMKDIVKQFPKVLANDRVNLSAKKGEIHAI
                     IGENGAGKSTLMNQLYGLYRQTSGDIYINGQKKDFSGPGDAIKAGIGMVHQHFMLVNN
                     LTVAENIVLGCEPLKDIRFDIKTARKQVKELSEKYGLIVDVDAVIEDLPVGTQQRVEI
                     IKTLYRGAEILILDEPTAVLTPQETQELFAIMKKLKNDGKTILFISHKLNEVITISDN
                     ITVMRQGKVTGRVSKDMTDERQLANMMVGREVVLNIEKPVKTPGETVVKVSDLWVKDN
                     RKLDAVEGLSFELRKGEVLGIAGVAGNGQTELVEALTGLRRIEKGQYIYKGQDFSRKD
                     VAQLRQAKITHIPEDRHKYAMIKDYPNYYNLILGRHLELPFSRRGFLRKKDIIENANS
                     MIERFDVRPKDSNNLSGNLSGGNQQKLVLAREIGANPDFIVIDQPTRGLDVGAIEYVH
                     KEIINLRQKDVAILLVSMELEEVLTLSDRIIVMYEGRAMGEFKPSELSIEEIGLMMAG
                     KTLEQITQPQNPGGDKK"
     gene            5558..6619
                     /locus_tag="PLU28_04035"
     CDS             5558..6619
                     /locus_tag="PLU28_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG34269.1"
                     /translation="MTVQSKNRAIKILVPVLSVIIALLIGAVVILAIGKNPVIAYKEM
                     FIGAFGSKMSWASNITKMTSLLLTGLAVAFGFRAGVFNIGAEGQMAVGGMAAAIVGIN
                     MGQTPMIIAVPVTILCSIMAGAVWASIAGILKAKTGAHEVITTIMLNWIAYHIGNYLV
                     VGPLAVGDGVPKSPEIAKSVQLPPLLTVQASTLPSGIIIAVAAAIIIYIILEKTTAGY
                     ELKAVGFNPYAAEYGGISISKNIILTMAISGGLAGLAGAMEVMCVHLRIFGSFTSGRG
                     FDGITIALIGQNSPIGSIFSAFLISSLRTGSTNMQFAGIPDDLVGLIQGIIIFLVAAE
                     RIIKTLIIKLFRVKGGENA"
     gene            6616..7566
                     /locus_tag="PLU28_04040"
     CDS             6616..7566
                     /locus_tag="PLU28_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG34270.1"
                     /translation="MNWFEGILAVFMSPLFYKLTITSALPLIFAALGGVYSEITGVTN
                     IALEGIMKMGAFVAIVFTLITGNPWIGLIFALISGVLMAALHAYVSIEWSANQVVSAT
                     ALVLIAEGLVGVLMPPIFGHEGSTNFFPKLPLIRIPGLKEVPFIGDIFGELSVFFYFA
                     VIAIFVSWFVIYKTPLGLRMRAVGENPKAADTLGVNVKKIRYFGVLMSGFLGALGGAY
                     LSIGEMGQFDDKMPSGKGFIAIAAMILGNWNPVGAMFATLLFGAAEAMNVQLQSMLSL
                     PSEVKALLNLLPFVITIIVVGGFIGKTRSPAASGVPYEKE"
     gene            complement(7732..7920)
                     /locus_tag="PLU28_04045"
     CDS             complement(7732..7920)
                     /locus_tag="PLU28_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34271.1"
                     /translation="MIRFTCNKHEDIFLSDVTIKFFETSIGGYWVYVSDETCVSNEKD
                     LKIRFNCAVVKDEMLTLA"
     gene            8033..8864
                     /locus_tag="PLU28_04050"
                     /pseudo
     CDS             8033..8864
                     /locus_tag="PLU28_04050"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            9027..9770
                     /locus_tag="PLU28_04055"
     CDS             9027..9770
                     /locus_tag="PLU28_04055"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG34272.1"
                     /translation="MVVPSVVSKLEAEDISFSFDQKSIIKNCSFSTRSGEITAIVGES
                     GSGKSTIMNMLLGLYKAQEGNIFIYDGIKSYNITEYGISGAGYLEQESMFFKEGTIKD
                     NILLGREVKADKFNEITLKTGVDDFVKKFEKGYDTLISEVSLSGGQKQLLAFARIMVD
                     DPQVLLLDEITSNIDSHTEEKIQELLKQEKGKNKLIIIIAHRLSTIKSADRILMLSDG
                     KICCEGKHEELLNTNEHYRNLVSIQTDKH"
     gene            complement(9840..11000)
                     /gene="trpB"
                     /locus_tag="PLU28_04060"
     CDS             complement(9840..11000)
                     /gene="trpB"
                     /locus_tag="PLU28_04060"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HOG34273.1"
                     /translation="MTETTMKKEGYYGSYGGMFVPPVLQEKLELLAGEFERAQADESF
                     IHQYRYYLNDYVGRPSPLYYARNLSEKVGAKIYLKREDLNHTGAHKINNAVGQALLAK
                     RMNARQIIAETGAGQHGVATATVAALFNIPCKIFMGKEDILRQKPNVQRMEMLGAEVI
                     ESTRGTMTLKDAVDDAFDYYVKNPDVFYLLGSAVGPHPYPAMVKFFQSVIGNETRYQI
                     LDKEGKLPENIFACIGGGSNAIGMFSAFLEDKQVKLFGAEGGGTELTNGNTAATLTLG
                     KPIVYQGTYSYCLVDENNIPVDAYSISAGLDYPGVGPEHSYLKDTQRVQYFPISDSEA
                     VDAFKLLSRLEGIIPAIESSHAVALALKILKSEPGLSVINLSGRGDKDLGRF"
     gene            11313..>11773
                     /locus_tag="PLU28_04065"
     CDS             11313..>11773
                     /locus_tag="PLU28_04065"
                     /inference="COORDINATES: protein motif:HMM:NF013514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOG34274.1"
                     /translation="MNGFDVQYFEKYKYVVGMDEAGRGPIAGPVFSAAVFVSNPDELK
                     FVCEIGADSKSLNEKERERRYRLITDNLIFVSSYVSAEDIDLYNIFKATQMSMLSNYE
                     KLFSAGREGYVIVDGKNFKFPFRNECIIKGDQKSSIIGAASIIAKYERDAY"
BASE COUNT         3649 a         1927 c         2503 g         3694 t
ORIGIN      
        1 attactgtat ttcctcgtag tttgagaact tctttcctgt aattgtctac aacataaaga
       61 tattcgccgt tgaaaaaccc tgccattggt ctgacacatg aggaataact gaaggattca
      121 agagtataaa tgtcaaatac agttactttc ccatcgtaat cacataccgt agcatagttt
      181 ttatgcgtat caatatgaaa tgcacgaggc ccgatatcga atctctctac tatctcaaag
      241 taatcctgcc ctgtcagtac tgtgcaaaat aaaatgacaa gcataaaaat cattataatt
      301 cttttttgaa ttttgatccc ctcctgataa aatataccta agcaatattt tatcatatat
      361 tcttggtttt atgaatgaac cttggaagta ttagtttttc tgattaacta aattatacta
      421 aaaacagaaa aatatagtat aattattgtg cattattaaa aattgtgtat ttatataaag
      481 gggtgaagct attggttcta gttgccatag tcgggggaag cggtgcagga aaaacaagtg
      541 ttgccaaaaa aattgccgag cattttgccg atagggcaaa tgtacttagt atggatgatt
      601 actatagaga ccttcccccg ggaatgacct taaaggaatt taattttgat gatcctaaag
      661 cttttgactt tgattatttt ataaaaaaca caaaaaagtt aaaagatgga agtccgatta
      721 atatacccgt gtataatatg gtgacttgca gaagggaagt cggtataagc acgagctttt
      781 ttcctaatga tctggttatt atagagggta ttttggttct tcatgagcct agattgagag
      841 agctttttga tttttctgtt tatatagatg ctcctgctga tgaaagactc ataagaagaa
      901 tagaaagaga taccatcgaa aggggaagat ccatagaaag tattcttacc cagtacagaa
      961 cttttgtagc cccttctttc agggctttta ttgaacccca gaaatataca tgcgatataa
     1021 tacttccgga tggcgtcagc aatattacgg gcatgaatat aataattaat gccatcgaaa
     1081 agatgcttga aattaaccga caggaggaag gaaaatgaaa aaaagtataa ttttcatgat
     1141 tttatttatc ttacttgtct ttacggtttg tttcgcaatg gacgatgcaa gagtgaagtc
     1201 ggagtatatt agttatattc agaagtatga aatgtttctt gctgacagca gccaggaaag
     1261 cgagcttaaa agcttttttg acgaaataaa agatatcggg ctttaccgtt tttacagaaa
     1321 cattatggta ggaaccgctg actatacaga tgttccgcag ggtgtacaga gatatctttc
     1381 agatacatca tcgagtcagg tttttgaaag cattgagcaa aaactagctt ttgcaggatt
     1441 actttgttat gtgcagacag atctttccgg tcagacgata acgagtgaaa taataagatc
     1501 acaaagagtc tttaacgatg caacttcgga gtatctaaga tcagtcgatg atgaaagatt
     1561 tacttatttt ggcaacgtta ttgcctattc actgggactt atagagtctt ctccttatac
     1621 agacataaaa aaatttagct tctcaggcaa gttagaatcc ccagaatact ttacttttac
     1681 aggagagcct atttttgact acgcaaagat tattaaagaa gaaaagcaaa ggatagaaga
     1741 aagcataaga aagatatccg aatcaggact gaccggttct gatcttgagt ttgagatcga
     1801 cagggcatcg gatgctattt cctctcatgc ggttgaaaaa gcaaaaagtg atatagactc
     1861 cgtaataaca attctaatag aaagtgctgt aagaggcaga gattactttc ttcttaggta
     1921 tatagtttat gtggctgcaa taatcatttt tcttatattt ttcaaaaaaa tatttaaata
     1981 tcttttagca gttattttta catcggaatt tttggttgtt ctttttacgt ttaacttttc
     2041 cgaagatgtt ataagctctt ttatatatgg aagttttatc attgtggcag tggtaattta
     2101 ctttgttttt atgcttttca ggtcattcgg aaaaggagtt tctgtttttt caaggatggt
     2161 taatattatt atttttgttt gtcttgtcct tttatttttt acaccatatg tttatgttcg
     2221 agatcttggg atggtcagaa acattgattt ttgcgactca aagtacagta cgtaccttct
     2281 taacgacact gttcttagca agaactctct tttattcaag gcaattcaga aaggtgacta
     2341 tgattcagcc aaaagacaga tttcgcgtat tgtaagttac ggcagtcctc agtataaaga
     2401 atttataaaa ccgcatatcc aaaaaataat atctgattat aatgtagggg ataagtttgc
     2461 gaactttgaa agagattttg aattgatgaa aagctctgag aacatagcag tttttgccca
     2521 caatactcag tatggaacga aggttttggt tttattcttc ctctcgctgt ttttgtttat
     2581 gttatcggag aaaggaatac tgaaaaaact atcgatcgca gcaattgctg catctgtggt
     2641 tctttccttt gtctctcctt caaaaatgat cattttaagc gaggtaaggt atccgatgct
     2701 aacaagtacc gagtattctt taaattttgt ttttaactta attatggtgg gaatgtccat
     2761 cctgctattt ttaaattcaa aatcaagagg agggaaaaaa gtatgaagaa acttttgttg
     2821 tttgtcatga ttgttactat gttagtatca agcgcttttt cgtttaaggc aatcatggtg
     2881 actgatacgg gtggattggg agataaatca tttaacgacg gaacatggtc aggtgttctc
     2941 agagctaaga acgaacttaa gattgaagct gaagtaattg tatcaaagga acagacggat
     3001 tatattccaa accttaccga tgcagcaaag agatccgatg ttgttcttgc tgttggtttc
     3061 cttatggcag atgctcttgc aaaaattgca cctcagtttc cggataagaa atttatcggt
     3121 atcgatatag ttattgatcc tattccttct aacgttgctc tttacaactt taaggaacag
     3181 gaatcagcat ttttggttgg ttatattgca ggttcagtaa ataaaacagg aaaagtgggt
     3241 tttctcggag gaataaaagt tcctgcagtt gccagatttg aaataggtta cagggctggt
     3301 gtggaagctt ataaccagct taagggtaaa aatacagaag ttctcatagc ttatgcaaac
     3361 catttcggtg atgcgccaaa gggtaaaagt ctttcactcg cacagatgga acagggagca
     3421 gatattgtgt ttgcagcagc cggtgcaaca ggaaacggtg cttttgaagc tgccaaagaa
     3481 aaggcacttg ctctttacgg acttaaagac ggagcatccc ttgatgctat aaaaaatgct
     3541 tataaaaaga ttggaaaagg ttattttgca ataggagtag acgttgacca agactactta
     3601 gcccctggaa taatactcgc atcagcaatg aaaggtattg atgttgctgc ttttgaaggc
     3661 ataaaatcag caatgggaag aaagtttgct tcaggtaata atgttgtggg tcttaaggaa
     3721 aacggagtca gactttctgc tcttacgtat accaaagata tgattgatcc tactgcactt
     3781 aaagagatcg aaaagttaat agaagatatt aaatctggaa agcttattat tcctgaagat
     3841 gaagtcggtc tgaaaaattt taaggtaaaa taaaaattgt ttgaatgggg tggggtatcc
     3901 ccatcccttt tatttacttt gccgatataa attttgtttg taccggcaaa gtaaataaaa
     3961 ctgaaatagc aggaggtgct gtaatgtctt ctactgctga aataaattat gaaaattacg
     4021 cagtggtgat gaaagatata gttaagcaat ttccaaaagt actggctaat gacagggtga
     4081 atctcagtgc aaaaaaggga gagatccacg ctattatagg cgagaacgga gctggaaaat
     4141 ctactctgat gaatcagctg tacggtcttt acaggcagac ttcgggagat atctatatta
     4201 atggacaaaa aaaagatttt tctggacccg gagatgctat aaaggctgga ataggtatgg
     4261 ttcaccaaca ttttatgctt gtgaataatc ttacagttgc tgagaatata gttcttggat
     4321 gtgagccgct taaagatata agatttgata taaagacggc aagaaaacaa gtcaaagagc
     4381 tctccgaaaa atatgggctc atagttgatg tagatgcggt tatcgaagat cttcccgtag
     4441 gaacccagca gagagttgaa ataataaaaa ctctttatag aggagctgaa atacttattc
     4501 tggatgaacc tacagctgtt cttactcctc aggaaactca ggaactgttc gcgatcatga
     4561 aaaaacttaa gaatgatggg aaaaccatat tattcataag tcataagctt aatgaggtaa
     4621 ttactatttc tgataatata actgttatgc ggcagggaaa agtaacgggc agggtctcaa
     4681 aagatatgac agatgaaaga cagcttgcta atatgatggt tggaagggaa gttgttctga
     4741 acatagaaaa gcctgtaaaa actcccggag aaaccgtggt taaagtatcc gatctttggg
     4801 tgaaagataa tcgtaaactt gatgctgttg aaggattaag ttttgaatta agaaaaggtg
     4861 aggtgctggg tattgccggt gttgccggga acggtcagac agagcttgta gaagcactta
     4921 ccgggttaag aagaatagaa aaaggccagt atatctataa aggacaggat ttttcaagga
     4981 aagatgtagc ccagttgcgt caagcaaaga taacacatat tccggaagat aggcacaagt
     5041 atgcaatgat aaaagattat ccaaattact ataaccttat tttaggacgt catcttgagc
     5101 tgcctttttc acgcagggga tttctgagaa agaaggatat tatagaaaat gccaacagca
     5161 tgatagaaag atttgatgta agacccaaag attctaacaa tctgtctggc aatctttccg
     5221 gcggcaacca gcagaagctt gttcttgcca gagagatagg agccaatccg gattttatag
     5281 ttatagacca gccgaccaga ggtttggatg ttggagctat agagtatgtt cacaaagaaa
     5341 taatcaatct cagacaaaaa gatgtagcaa ttcttcttgt ttctatggaa cttgaagagg
     5401 tactgaccct ttcagacaga ataattgtaa tgtatgaggg cagagcaatg ggtgagttta
     5461 agccgtcaga gctttcaata gaagagatag gactcatgat ggccggaaag actttagaac
     5521 agataactca gccgcaaaac ccgggaggcg ataagaaatg actgttcaat ccaaaaatcg
     5581 agccataaaa attttagttc ctgtactttc agtaataata gctcttttaa taggggcagt
     5641 tgtcatctta gccataggaa aaaatcctgt tatagcatat aaagaaatgt ttataggggc
     5701 ttttggttca aagatgtcct gggcctccaa tataaccaag atgacaagct tacttcttac
     5761 cggtcttgca gtagcttttg gtttcagagc tggagttttc aatattgggg cagagggtca
     5821 gatggccgtt ggaggtatgg cggcagcaat agttggaata aacatgggtc agacacctat
     5881 gataattgcc gttccggtaa caatactttg cagtataatg gccggagcag tatgggcaag
     5941 catagccgga attttaaaag ctaaaacagg ggcacatgag gttattacaa ccataatgct
     6001 gaactggata gcatatcata ttggaaatta tcttgttgtg ggtccgcttg ctgtcggtga
     6061 tggtgttccc aaatcgccag aaatagctaa aagtgtgcag cttccaccac tactcacggt
     6121 ccaggcaagc actttgcctt caggtataat aattgcagtt gcagctgcta ttattatata
     6181 catcattctt gaaaaaacaa cggctggata tgagcttaag gccgtagggt tcaaccctta
     6241 tgctgccgaa tacggcggaa tatcgataag caaaaatata attcttacaa tggctatatc
     6301 cggtggcctt gccggacttg caggggctat ggaagtcatg tgcgtacatt taagaatttt
     6361 tggatctttt acaagtggaa ggggatttga cggtatcact atcgcactta taggtcaaaa
     6421 cagtcctata ggaagtattt tttcggcttt tcttatttct tcattacgta cggggtcgac
     6481 caatatgcag tttgcaggaa tacctgatga tcttgtaggc ctgatacagg gaataatcat
     6541 ctttcttgtg gcagcggaaa gaataataaa aactttaatt ataaagcttt tcagggtgaa
     6601 aggcggtgaa aacgcatgaa ttggtttgaa ggtattttgg ctgtattcat gtccccactt
     6661 ttttataagt taactataac ttctgcgctt cctcttatct ttgcggcttt gggtggagtg
     6721 tacagtgaaa taacaggagt aactaatatt gcgcttgaag gaataatgaa gatgggagct
     6781 tttgtggcaa tagtatttac tcttattaca ggtaatccgt ggattggatt gatatttgcc
     6841 cttatatccg gagttctcat ggcagcactt catgcgtatg tatccataga gtggtcggcc
     6901 aaccaggttg tttcagcaac agcgctcgtg cttattgccg aaggacttgt cggcgttctt
     6961 atgcctccta tcttcggaca tgaaggctct acaaactttt ttccgaagct tcctcttata
     7021 agaattcctg gactcaagga agtgcctttt ataggagaca ttttcggaga actttctgta
     7081 ttcttttact ttgcggtaat agccatattt gtatcatggt tcgtaattta taaaactcct
     7141 ctggggctca gaatgcgtgc cgtcggagaa aatcccaagg ctgctgatac ccttggagta
     7201 aacgtcaaaa agatcaggta ctttggagtc cttatgagcg gttttctcgg agctttgggt
     7261 ggagcatacc tttcaatagg agaaatggga cagtttgatg acaaaatgcc atcaggtaaa
     7321 ggatttatag caatcgccgc catgatactg ggaaactgga atcctgtagg cgctatgttt
     7381 gcaacccttc tttttggtgc tgcagaagca atgaatgttc agcttcagtc tatgctgagt
     7441 cttccttcag aggtaaaggc attgcttaat ctcctgcctt ttgttattac aatcattgtg
     7501 gtcggtgggt ttatcggtaa aacaagatct cctgctgcaa gcggtgttcc atacgaaaaa
     7561 gaatagataa tataaaaact gcgattttta aagaatcgca gtttttttta tttaaattta
     7621 aaataggcat tttatagttt gtttagtgaa ttaaataaaa ataagatatt tttatgatgt
     7681 aataaaaata attatttata ttttgttgaa ataatggttt tcaatgtaca gttaggcaag
     7741 agtaagcatt tcgtctttga ctactgcaca attaaaccgt attttcagat ctttttcatt
     7801 tgatacgcag gtttcatcac tgacatagac ccaatatccg ccaatgcttg tttcaaagaa
     7861 ttttatggtt acatcagaaa gaaagatatc ttcatgctta ttacatgtga aacggatcat
     7921 ttttaaagac agatctaaaa gctttataac ctacctcgtt aattttaaag ttatcctatg
     7981 atactagaat aaagcaaaga tagtgtttaa aatactttgg ggggtgtgaa agttgactga
     8041 tgaaaacaag aaagaaaaaa gggtacttaa ccagtttgtc agaggactta taggccagta
     8101 caagtacagt cagatactta ctttttttct tatatcttta caggcagccg gaaatattgt
     8161 tctgcctctt ctgtttcagc agatagtgga caagtctctt ccttcaggcg atatgtcctc
     8221 tcttatgcat tatattatga ttcttgttgt agtcatggtt gtggctcttt ctactggtta
     8281 tctcggggaa attctttttg agtttacagg acttaaagta aagaaagaac tcaacaggcg
     8341 tgtaataaaa aaatttcttg atgttcctta cagctttttt tatcaggaac agttccggag
     8401 aactggccac tcatcttata tcggatatag ataatgtgac catgatggtt tcacagatac
     8461 taccgattac tttgctttct ttaatgcaga ttgcaggtat tcttttcata atatttttta
     8521 tgaactggaa gcttgcgctt ataccagtta ttctgttctt cgttttttat atgcttgttg
     8581 ctgtaataaa caaacaggct cagaagaaga gtttaaccga gagaactctt tttggtaaga
     8641 tgggggctgt tataataaat attcttgaaa atattaagtt cattaaggtc ggtaagcctt
     8701 atggctggct taacagttat tttgagtcgc atcaggaaaa ctatataaag agtaaaaaga
     8761 agcttgtctt ggtgcttaag cttgtaggga cgcttggaaa cggtatgttt ggaataatcg
     8821 cttttgtcgt tttttcaatt ggaagctata tggtcataaa agatatgctt actgtagggc
     8881 agcttcttgc cttttgggct tatatacagg ctatagccgg gcctttacag cttatgatac
     8941 aggtcaatat gtttaagatg agctcctatg gctctgtaaa gagagttatg ggtattcttg
     9001 atgaaaaaga ggaaaagcgc tcagatatgg tagttccgtc tgttgtatcg aaacttgaag
     9061 ctgaagatat aagcttttct tttgatcaga aatctataat aaaaaactgt tctttcagta
     9121 caagaagtgg tgaaataaca gctatagtgg gcgaaagtgg ttcgggaaaa tctactataa
     9181 tgaatatgct tttgggttta tataaggctc aggaaggaaa catttttatt tatgacggca
     9241 tcaaaagcta taatataact gagtatggaa tcagtggagc tggttatctt gaacaggagt
     9301 ccatgttctt taaggaagga accataaaag acaatattct tctgggaaga gaagtaaaag
     9361 cggataagtt taatgagatt actttaaaaa ctggagtaga tgattttgta aaaaaatttg
     9421 aaaagggata cgatacttta ataagcgaag taagcctctc cggaggtcag aaacagcttc
     9481 tggcttttgc aaggatcatg gtggatgatc cgcaagtgct gctgttggat gaaattactt
     9541 ctaacattga ttcgcatact gaagaaaaga ttcaggagct tttaaaacag gagaaaggaa
     9601 aaaacaagtt gattataatt atcgcacaca ggctttccac aataaaaagt gctgacagga
     9661 tacttatgct cagtgatgga aagatatgct gcgaaggcaa gcatgaagag cttttaaaca
     9721 ctaatgaaca ttacaggaat ctggtttcta tacaaacgga taagcattaa taaaaaccgg
     9781 aaggacagat taattaagtt taactgtctt tccggttttt ttggtagtac ttggtgcttt
     9841 cagaatcttc ccagatcttt gtctcctctg cctgataggt tgatcaccga tagtccgggt
     9901 tctgacttaa gaattttcag tgcaagagcg acagcgtgtg aggattctat tgctggtatt
     9961 atgccttcca gtcgtgacaa cagtttgaaa gcatccacag cttcgctgtc acttattgga
    10021 aagtactgta ctctctgagt atctttaaga tatgaatgct ccggtcctac tcctggataa
    10081 tcaagacctg cggagataga gtacgcatct acaggaatat tattttcatc gacaagacag
    10141 taagagtagg ttccctggta tacgatgggt ttgcctaaag taagtgtggc tgcagtattt
    10201 ccgtttgtaa gctctgtacc tccgccttcg gctccgaaaa gtttaacttg tttatcttct
    10261 aaaaatgctg aaaacattcc tatagcattt gatcctccgc ctatgcaggc aaaaatattt
    10321 tcagggagct ttccttcttt atctaaaatc tggtagcgtg tttcattacc tataaccgac
    10381 tgaaaaaact taaccatagc cgggtaagga tgggggccaa cggctgagcc aagcagataa
    10441 aatacatcag gatttttgac ataatagtca aaagcatcat cgacagcatc ttttaaagtc
    10501 atggttcccc ttgtactttc aataacctcc gctccgagca tttccatcct ctggacatta
    10561 ggtttttgcc ttagtatgtc ctcttttccc ataaatattt tgcatggtat attgaacagg
    10621 gctgcaaccg ttgcagttgc aacaccgtgc tggccggctc ctgtctccgc aattatctgt
    10681 ctggcattca ttctttttgc taaaagggcc tgtcctacgg cgttgtttat tttgtgagca
    10741 ccggtatggt taagatcttc ccgtttgaga tagatctttg ctccaacttt ttcagagagg
    10801 tttctggcgt agtataaagg tgaaggtctg cccacgtaat cattgagata gtagcgatac
    10861 tgatgtataa agctttcatc agcttgggct ctttcaaact caccggcaag cagttcgagt
    10921 ttttcctgta gaacaggtgg tacaaacatt cctccgtaac ttccgtaata gccttctttt
    10981 ttcatagttg tttctgtcat agtatctcct cctgtaaaat aaaatttggg cttctccttt
    11041 tttatgtgga gaaacccaat aaaaaaggca ccttttagtt aaaaaggtgc ccgttatttt
    11101 tatattttca gatataaata cggaatgagc accttataaa agatgccacc accatctgtt
    11161 ggatatgctt attgaattga aggatgtatt atttttatac atagttagca ctcctaataa
    11221 tatttgtatc cattctatca tacaatagat aaactgtcaa gcatctttga aaaagcttta
    11281 tgctatgata aaattatctg ggaggcgata tattgaatgg gtttgatgtg caatactttg
    11341 aaaaatataa gtatgtcgtc ggtatggatg aagccggacg cggacctata gcggggcctg
    11401 tcttttccgc agctgttttt gtttcaaatc cagatgagtt aaaatttgta tgtgagatag
    11461 gtgcagattc taagtcattg aatgagaagg aaagagaaag aagatacaga ctcataacgg
    11521 acaatcttat ctttgtaagc tcttatgtaa gtgccgagga tattgattta tacaatatat
    11581 tcaaagccac gcagatgagt atgctgagta attacgaaaa acttttttct gccggcaggg
    11641 aaggatatgt tattgttgat ggtaagaatt ttaagtttcc gttcaggaat gagtgtataa
    11701 ttaaaggaga tcagaaatca tcaataattg gtgcagcttc aattattgcc aagtatgaac
    11761 gggatgcata cat
//
LOCUS       DAOSHR010000049        11632 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_4654_length_11632_cov_6.120670, whole genome shotgun sequence.
ACCESSION   DAOSHR010000049 DAOSHR010000000
VERSION     DAOSHR010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 11632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11632
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4654_length_11632_cov_6.120670"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /locus_tag="PLU28_04070"
     CDS             complement(<1..499)
                     /locus_tag="PLU28_04070"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOG34275.1"
                     /translation="MVNSISEIAFQTNLLALNAAIEAARAGEHGKGFAVVAQEVKKLA
                     EQSKESASSISEILGQIQNQIHDAVSSIDKSTEEVSSGQKVVLVAGESFFSIEKQIAE
                     LSEAVDGITESINSLYDYNSNVLDAVGKIRDISQETASNSKTISMATEEQSAGVEEIA
                     SSAESL"
     gene            complement(581..1981)
                     /locus_tag="PLU28_04075"
     CDS             complement(581..1981)
                     /locus_tag="PLU28_04075"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOG34276.1"
                     /translation="MGNRLKEKKSLSIRIKLSLLIVSLIVLSVSVIELYSYFSRAADI
                     ETAVRKEQLNTAILTASRLETEIARTVSVLQTAACNEAFISEDKNTIIKALKNIKDQN
                     EIFSTVFLVDSMLNKLNEKSETGSLATREYMQEVKKIQKTVISHEILISQSTKKPSIM
                     IATPIKINGAPESYLGISVNTDYLQRIVNEVKSSESEYTFAFDGKNGLVFAHPDAEYI
                     GKLKVLNPDEPDKKLIAPQLREAVELATNGNSGSTVYTFNGEKIIAAYANIPGTNLGV
                     VTRMTHQNAMAPIRRERNISLLITFIASLLGFALAYFLSKFISDPIKKVSNMSKIISK
                     GDFSKTSDIVKSKNNDEISCLQESFGVMADMLKTTMHQIHDATIQIASASGELQISSE
                     KSAQASEQVAVAVSEVSQGAVNQAEAADKTLQAVKKMSERVKDIAIHACSAENVSKSS
                     SLAVESGEKAINHAVQ"
     gene            complement(2402..2932)
                     /locus_tag="PLU28_04080"
     CDS             complement(2402..2932)
                     /locus_tag="PLU28_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009064821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOG34277.1"
                     /translation="MDFFELAKSRCTTRGFNERTIPDEDLMRILSIGRVAPSACNKQP
                     QRILVVREPQNILKVKKAYNTFGSKCVLIVCRDTRDSLVRPYDQKCSGDLDIGIVCDH
                     MMLAARELGIGSVMVGLFDPYIIRREFEIADYIIPTALLILGYPRDGFLSPQRHDTQR
                     KPLSDTVMFEKFSLNC"
     gene            complement(3110..5623)
                     /locus_tag="PLU28_04085"
     CDS             complement(3110..5623)
                     /locus_tag="PLU28_04085"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOG34278.1"
                     /translation="MAIKYSNPEKRPNIFFIFLIVIVFIGGIFYIYASWYHYSSIRRS
                     EVIRLAVSASAFIEPMHLKELKNDSSDLQNEYYNTLKTNMISFAKKNKKIKFAYLLAL
                     KDNRLFFIVDSETPSSKDYSPPGQEYTEITDYDWLPFSTGEPVLKNNITDRWGTWVSV
                     FVPIKDHYLKNVVAVFGMDYPVNSWTKDTLIYMIQPSLIVAFILILILMLYRALSIQK
                     KFNKTFRELKDSELLFRSVFTQAPVGIALLRDYKKHEVSNDELDIIDSNEEFLRIVSR
                     KKNDDPCFDWKSITHPDDLSKDTEMFEQYKSGKFTGYSLEKRYIFPNGSVKWVNMMIR
                     SLDISEGSKENKYHLCIIKDITDRKKAEETLKESERSKSVLLENLPGMAYRCKNDRQW
                     TMEFVSKGCFDLTGYYAENLINNRDLSYNDLIADDYKEIIWDEWKRVLELHEPFRYEY
                     EIVTSSKKHKWVLETGQGIFRKDGSIEALEGIIIDITESKMRQYQIEYANDHDFMTGL
                     FNRRYYETQKERLDKSGNTPVSILVADINGVRMINDTFGHIQGDILIMETAKLLQKCC
                     RESDILARIGGDEFAIILPYTSREKAFEILKNIRKTFDNFNSLTSETKNRISLSMGYG
                     TKDDLNASLQNAEKEADEYLRRRKIFEQKSLHNSVLSSIMATMYARSQETEEHSQRIS
                     ENCIKVAERLGLAQSAQDELHLFAMLHDIGKVGIDDRILNKPGKLNDEEWTIMKTHPE
                     IGFRIVMSVPELSGVANYILTHHERWDGKGYPLGISGEDIPILSRILAVADAYDAMIE
                     SRVYRKALSKEEALNEIKNNSGTQFDPNISNIFIEILRI"
     gene            complement(5880..6506)
                     /locus_tag="PLU28_04090"
     CDS             complement(5880..6506)
                     /locus_tag="PLU28_04090"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HOG34279.1"
                     /translation="MSIPDQKIYPRTSDKSTVYLKPLIKNPSILIGDYTFYNDFKNDP
                     TDFEKNNILYHYPINHDRLIIGKFCSIACGAKFIFTSANHTLNSFSTYPFPIFWEEYE
                     TLPVNIVRAWDNKGDIVVGNDVWIGYEAVIMSGVHIGNGAIIGTRAVVTKDVEPYAIV
                     GGVPAKIIRKRFKDDVIKKLQMTSWWDWDIEKIKKKLKFLTTMDIENL"
     gene            complement(6604..7098)
                     /locus_tag="PLU28_04095"
     CDS             complement(6604..7098)
                     /locus_tag="PLU28_04095"
                     /inference="COORDINATES: protein motif:HMM:NF024304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOG34280.1"
                     /translation="MFRKMRRINQLLSDQETQDVLKRCTHGVLACLGDEDYPYAIPLS
                     FVYFNNKVYFHSAKQGHKIDAVSKNPKVSFSVIDEDTVISKEYTTYFRSVIIFGKARI
                     VQGDERTEAFMALIEKYSGDQPEESKQKEANSCMQSLILAVDPEYITGKQSIEFVRNQ
                     KRQS"
     gene            complement(7117..8043)
                     /locus_tag="PLU28_04100"
     CDS             complement(7117..8043)
                     /locus_tag="PLU28_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG34281.1"
                     /translation="MDEFKYVYGPVPSRRLGISLGVSPIPKKTCNYSCIYCQLGRTTR
                     MINHRQLFYPVDNIIAELKTFFQKNINYDVITIVGEGEPTLYSGLGQLIEKLHKLTNK
                     PIAVITNGSLLYDPDVQSDLMQSDILLPTLDAYDEKSFKKINRPYSSLNYYKVLNGLE
                     DFSRKYKGQLWLEIMFMKGINDDPNTIIKYSEILKKIKYEKLYLNTPVRPPAEIDVFA
                     LDHEKMEHIAEILNGFSIDLLSSHGFYSEIKNDYEAFVSIIKRHPMNQFEIEEFLKSR
                     GNSFPEKFMENLETDASLAIIEYKGYRTYRLK"
     gene            complement(8125..8637)
                     /locus_tag="PLU28_04105"
     CDS             complement(8125..8637)
                     /locus_tag="PLU28_04105"
                     /inference="COORDINATES: protein motif:HMM:NF017690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOG34282.1"
                     /translation="MNEIKLNSLINAVDSFFKKNPDADFDKLDLFLKENFDQTDIDAY
                     YEMLGQTLGSMDGVMENPMNAFLEGPKEKVKSYPGVTVVEFPMEERLRSLAVKEWEIW
                     EKDISEFDHIYDSTETFFVLEGRALLEFKTHKVEVEAGDLVQIEKGAKCHWSIKEPVR
                     KYYTFDIKEI"
     gene            complement(8634..8933)
                     /gene="fliE"
                     /locus_tag="PLU28_04110"
     CDS             complement(8634..8933)
                     /gene="fliE"
                     /locus_tag="PLU28_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HOG34283.1"
                     /translation="MIERINGLKGLHNLEIQKNKKTTGQNDLNFSQMLKDAINEVNDL
                     QVKSDKMTADYAAGRITSIEQVTVAAEKASLSLKLTTEVTNKVIEAYKEIMRLQL"
     gene            complement(8947..9375)
                     /gene="flgC"
                     /locus_tag="PLU28_04115"
     CDS             complement(8947..9375)
                     /gene="flgC"
                     /locus_tag="PLU28_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HOG34284.1"
                     /translation="MESFFRSMNVAASGMTAERMRFNVIAQNIANAETTRTEDGGPYV
                     RKYVTFREILDSRFNDDDANSFSGVQVDSIEEDPAPFRLVYDPTHQDADADGYVRYPN
                     VVALKEMVDMMTAQRAYDANTAVISTARSMFNSALNIGRQ"
     gene            complement(9379..9777)
                     /gene="flgB"
                     /locus_tag="PLU28_04120"
     CDS             complement(9379..9777)
                     /gene="flgB"
                     /locus_tag="PLU28_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HOG34285.1"
                     /translation="MFDLTFSILPQAMNAASVRNSVIAQNIANQSTPDYKRKYVTFEE
                     EFQKALGSSDEIALKTNKPSHINNTPSLTQVQPQILKDTSRSNREDGNNVNVDVESVN
                     MYINGMYYNGLTRLMNYSIQRYNTVIKGVR"
     gene            9844..10881
                     /locus_tag="PLU28_04125"
     CDS             9844..10881
                     /locus_tag="PLU28_04125"
                     /inference="COORDINATES: protein motif:HMM:NF016145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HOG34286.1"
                     /translation="MGKIFLVGYYGYNNFGDELLLNSMLKILNDVKFQGEVIIPSDNL
                     LKIETPISYKVTDIKKFDLYNIVLSIKSSDLVIFGGGNLFQTETSLKSFLYYYYIAKK
                     AIKYKKPLFLISQGFGPIKNKFANKLLKSLLKTENAQIYGFYRDEASFRYARRYCKSS
                     FNIVDIAPYAISDIGLRAQTTDITSICIKKDYDDFMPLYEFLEIDNRSINTLAINSNQ
                     DSEISFKFVDYLRSVSDWSVPFPTGDNKKIQSTLINSKLVISDRLHSAVVPMYFGVPT
                     VVSASAKNRRVVKNICPDYDYFYKKLSDIPGAYAYMKARNYDPQKTAAVYREKLSEAV
                     QTIEQNLRTFL"
     gene            complement(10865..>11632)
                     /locus_tag="PLU28_04130"
     CDS             complement(10865..>11632)
                     /locus_tag="PLU28_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34287.1"
                     /translation="VDNYRKEVLKLRGNTVIKRVSLGSNPVNMKTIDNKIFISAFDPD
                     KLFVFDKNLSLIQSIDLSVKSPFLQIAGKDLYIPMRENTKDNTITTRFLFYNATVGSY
                     IVNYQNIRSPIDILEYNNRLFIASYYNGKIYENLVAGQKEVAAFGGYLINIELFNSYI
                     VGNSLFGGIYFYDIEKKGIVRMLEDIPIVDIAVSPSKNYIFALSSIENKLFIIKDFQV
                     YQEIKLDDYPIDVEVPDESVIIVLSTQAGKVQVIRKF"
BASE COUNT         3505 a         2131 c         1941 g         4055 t
ORIGIN      
        1 ccaatgactc tgctgatgaa gctatttctt ctactcctgc cgactgttct tcagttgcca
       61 tagagatagt cttactatta gaggcagttt cttgagagat atctcttatt tttcccactg
      121 catccagaac atttgagttg tagtcataaa gtgaatttat actttctgtg attccgtcta
      181 ccgcttctga aagttcagct atctgttttt ctattgagaa aaaggattca ccggcaacta
      241 atactacttt ttgtcctgag gatacttctt cagtgctttt atctatactg cttacagcat
      301 catgaatctg attttgaatc tgaccaagaa tttcagatat actagatgcc gattcctttg
      361 actgctctgc taacttttta acttcctgag ccacaacggc aaaacctttt ccgtgttctc
      421 cggctctagc agcttctatg gctgcattta aagctaaaag attcgtctgg aaagctattt
      481 ctgatatgga attaacaatt tcacttattt tatctgaaaa agcagcaagt cttcgtatag
      541 tttcagcggt gctttgtact atggaactaa tagcagttat ttattgtaca gcgtgattta
      601 tagctttttc acctgattca acagctaatg aagaagattt ggatacattt tctgctgagc
      661 aagcatgtat agctatatcc ttaactcgtt cactcatttt tttaactgcc tgtaaggttt
      721 tgtctgctgc ttctgcctga tttactgctc cttgtgatac ttctgatact gcaacggcaa
      781 cctgctcaga tgcttgtgca gatttttcag aacttatctg aagctctccg ctggctgaag
      841 ctatctgaat agtagcatca tgtatctggt gcatggtagt tttgagcata tctgccatta
      901 ccccgaagct ctcttgtagg caacttattt catcattatt tttacttttt actatgtcag
      961 aagttttcga aaaatctccc ttagagatta ttttggacat gttagaaact ttttttattg
     1021 ggtctgaaat aaattttgat aaaaaatatg ccaatgcaaa acctaataaa gaggctataa
     1081 aggttatgag caatgaaata ttacgttccc ttctaatagg agccattgca ttttgatgtg
     1141 tcatccttgt tacaacgcca agatttgttc cgggtatatt tgcatatgcg gctattattt
     1201 tttcgccgtt gaaagtataa actgttgatc ctgaatttcc gtttgttgct agttcaacag
     1261 cctctctcag ctgcggagcg ataagcttct tatcaggttc atcaggattg aggaccttga
     1321 gtttcccgat gtattctgca tctgggtgtg caaaaaccaa accattcttt ccgtcaaaag
     1381 cgaaagtata ctcactttca cttgatttta cttcgtttac aattctttgc aggtagtctg
     1441 tattaactga tattccaaga taactctctg gggcgccgtt aatctttatt ggcgttgcta
     1501 tcataattga aggttttttt gtagattggg atataagaat ctcatgcgag attactgttt
     1561 tttggatttt tttgacttcc tgcatatact ctctggttgc aagcgaacct gtttctgatt
     1621 tttcatttag tttgttaagc atggaatcca ccagaaaaac agttgaaaat atttcatttt
     1681 gatcttttat attctttaaa gcttttatga tagtattttt atcttcagaa ataaaagcct
     1741 cattgcacgc tgctgtttgc agaacagaga ctgtacgtgc tatttcggtt tccagacgtg
     1801 aagctgtcag aatagcggtg ttaagctgtt cttttcttac agctgtttca atatctgctg
     1861 ctcttgaaaa ataactataa agctcaatta cagacacaga tagtacgata agacttacta
     1921 taagcagtga gagtttgatt ctgatgctta gtgatttctt ctcttttaaa cggtttccca
     1981 ttttacagtg cctcccatga agttttagca tatataagct tgtatgtgtc aaataaagaa
     2041 agttaaacta taaggttctt gtaattacta ggttcagaca cagtcttata atgaatgaaa
     2101 actaagtaaa taatcataat aaaaggattt caaaagttat tatacctctc attctttttt
     2161 tttcatgatt tttatgtttc ctgtttttaa cgtaattcaa aacagataag tataaaaaag
     2221 tagagtacag taatctttca gggtaagtac tttataatcg ttgctgttaa gctagaagtt
     2281 attaagtaag aacctttttg tttagagaat aaaaatccaa aacccggaag ccttatatat
     2341 gaggttttcc gggttttaaa aattcagatt atcaggcggc tttgaatttt ttcaaagttt
     2401 cttaacaatt taaagaaaac ttttcaaaca ttacagtatc tgaaagtggt ttacgttgtg
     2461 tatcatgacg ctgaggactc aggaaaccat cacgaggata tccaagaatc agtaatgcag
     2521 ttggaattat gtaatcagca atctcaaact cacgtcttat tatatacgga tcaaaaagac
     2581 ctaccataac gcttcctatt ccaagttctc gggcagcaag catcatatga tcacaaacga
     2641 ttccgatgtc caggtcacct gagcattttt gatcgtaagg gcgtacaaga gaatcgcggg
     2701 tatcacggca aacaataagc acacattttg aaccaaaagt attgtatgct tttttcactt
     2761 taagaatatt ttgaggttct cttactacca aaatcctttg gggttgcttg ttacatgcgc
     2821 tgggagcaac acgtcctata gagaggattc tcattagatc ttcatcaggt attgtccttt
     2881 cattaaatcc tcttgttgtg catctgcttt tagctaattc aaaaaaatcc atacttattt
     2941 ttcatctcct catgcagtgt aagacaaaag ttaattaaac ttttgtatac caataaattg
     3001 gtatatctac tatacttttt ttatgttaag caaaaggatt actttatgat aataatcgta
     3061 aaacttggta aaatataatg cgctatcaag caaaaaccat catgagaggc taaatacgta
     3121 gtatttcaat aaaaatattt gaaatattag gatcaaactg cgttccggaa ttgtttttta
     3181 tttcatttaa tgcttcttct tttgacaagg ctttcctgta aacccttgat tctatcatag
     3241 cgtcataagc atctgctaca gcgagtatac gagaaagaat aggaatatct tctcctgaaa
     3301 taccaagagg ataacctttt ccatcccagc gttcatgatg agtaagaatg tagttggcaa
     3361 cgccggagag ttcggggaca gacataacta tacggaaacc gatttcagga tgagttttca
     3421 taatagtcca ttcttcatca tttagcttgc ctggtttgtt cagaatacgg tcatcaatac
     3481 ctactttgcc tatatcgtga agcatggcaa aaaggtggag ctcgtcttgt gcgctttgtg
     3541 caaggccaag tctttcagcg acttttatgc agttctcaga aatacgttga gagtgttctt
     3601 ctgtttcctg acttcttgca tacattgtgg ccatgatgga ggacagcaca gaattatgga
     3661 ggcttttctg ttcaaaaatt tttcttcttc gtaaatattc atccgcttct ttctcggcgt
     3721 tttggagact tgcgtttaaa tcatccttag ttccatatcc cattgaaaga cttatacggt
     3781 ttttggtctc tgatgtcaat gaattgaagt tatcaaatgt tttccttata tttttaagaa
     3841 tttcaaatgc cttttctctt gaagtgtaag gaaggataat tgcgaattca tcaccaccta
     3901 ttcgtgcaag aatatcactt tctctgcagc atttctgcag aagtttagca gtttccataa
     3961 tgagaatatc tccctgaata tgaccaaaag tatcgttgat cattcttact ccgttaatat
     4021 cggcaactag tatggatacg ggggtatttc cacttttatc caaacgttct ttctgtgttt
     4081 cgtagtatct tctattaaac aggccagtca taaaatcatg gtcatttgca tattctattt
     4141 gatattgacg catctttgat tcggttatat ctattataat accctccaga gcttcaatac
     4201 tgccatcctt gcgaaatatt ccctgtcctg tttcaagaac ccatttgtgc tttttagaag
     4261 aagtgactat ctcatattcg taacggaatg gttcatgaag ttcaagaact ctcttccatt
     4321 cgtcccatat aatttctttg taatcatctg caataaggtc gttgtatgaa agatccctgt
     4381 tattaataag attttcagca tagtatcccg taagatcaaa acagcctttt gatacgaact
     4441 ccattgtcca ttggcggtca ttcttacatc tataagccat accagggaga ttttccaata
     4501 gaacactctt gcttctttcg ctttctttga gagtttcctc tgcctttttt ctgtcagtta
     4561 tatctttgat tatacataaa tggtacttat tttctttaga cccttcagaa atatcaagac
     4621 ttcttatcat catatttacc catttcacag aaccattagg aaaaatataa cgcttttcca
     4681 gagagtaacc ggtaaatttt cctgatttat actgttcgaa catctctgta tctttagaaa
     4741 gatcgtcagg atgagttatg cttttccagt caaaacaagg gtcatcgttt tttttccttg
     4801 aaactattct taaaaattct tcgttcgaat caataatatc taattcatca ttcgatactt
     4861 catgtttttt ataatccctt aagagagcta taccaaccgg agcttgtgta aacactgatc
     4921 tgaagagcaa ttcactatct ttaagctcgc gaaatgtttt gttaaacttt ttctgtattg
     4981 acagtgccct ataaagcatt aagataagta tgagaatgaa agcaactata agtgaaggtt
     5041 ggatcatata aatcaaagta tccttggtcc atgaattaac aggataatcc attccaaaaa
     5101 cggcaacgac atttttaaga taatgatcct ttataggaac aaaaacactt acccatgttc
     5161 cccagcgatc ggttatattg ttttttaaaa caggttctcc ggtagaaaaa ggaagccagt
     5221 cataatcagt tatttctgta tactcctgtc ctggcgggga gtagtctttt gaggaaggag
     5281 tttcagaatc aacgatgaaa aaaagcctgt tatctttaag agcaagaaga tatgcaaatt
     5341 ttatcttttt gttttttttc gcaaagctta tcatgtttgt ttttagagta ttatagtatt
     5401 cattctgaag gtctgagcta tcgtttttta actctttcaa atgcattggt tctataaatg
     5461 ccgaagccga gaccgccaaa cgtataactt cgctacgtct tatcgatgaa taatgatacc
     5521 atgaagcata tatatagaat atacctccaa taaaaactat aacaattaaa aaaatgaaga
     5581 aaatgtttgg tcttttttct ggatttgaat attttatcgc cataagagac ctccattaaa
     5641 taaaaaatga gtctattatt tcatatgctc cgtataaaaa attatactat atttatgaac
     5701 tgtaatcaac ttaaagttgc taaatgcata atattaggta atgaaaagtt aacgtttatt
     5761 ttttaaacaa agaaagaatt ttttgatgat aaacaaatat aaagaaaaat atttcaggca
     5821 ttaaaaaaga taaccgggat tatcccggtt atctcataag ctcaagaaat taatggatat
     5881 tataaattct caatatccat agtagtaaga aatttaagct tctttttaat tttttctata
     5941 tcccaatccc accagcttgt catctgaagt tttttgatga catcatcttt aaatcttttt
     6001 ctgataattt ttgctggaac tccgcccaca atagcatatg gttccacgtc ttttgtaaca
     6061 acagccctag tccctatgat agctccatta ccaatatgta cacctgacat tataactgcc
     6121 tcatatccta tccagacatc atttccgact actatatctc ctttgttatc ccatgctcgt
     6181 actatattta ctggaagagt ttcgtattcc tcccagaaaa tcggaaacgg ataggttgaa
     6241 aaagagttta gagtgtgatt agcactagtg aaaataaatt ttgcaccaca cgcaatggaa
     6301 cagaattttc caataataag cctgtcatga tttataggat agtgatatag aatattgttt
     6361 ttttcaaaat ctgtaggatc atttttaaaa tcgttataaa aagtgtagtc gcctatcaat
     6421 atactgggat ttttaattaa tggttttaaa taaacagtac tcttatcact ggtccgggga
     6481 taaattttct gatccggaat agacattata caacctcctg atattaaaaa gcattttgag
     6541 atctatcccg gaatttacac tgcatttctt catactgcac catccattaa attaatatgg
     6601 tttttatgac tgtctttttt gatttctaac aaattcaata gattgtttgc cggttatgta
     6661 ttctggatct acagctaata taagcgattg catacaactg tttgcttctt tttgttttga
     6721 ttcttctggc tggtcgcctg aatacttctc aatgagtgcc atgaaggctt cggttctttc
     6781 gtctccctga actatcctgg ctttaccgaa gataatgaca cttcggaagt aggttgtata
     6841 ctctttactt atcacagtat cttcgtctat tactgaaaat gaaaccttag gattcttaga
     6901 aacagcatct attttatgtc cctgtttggc agaatgaaag taaactttat tattaaaata
     6961 gacaaaactt aatggaatgg catatggata atcttcatcg cctaagcagg ctagaactcc
     7021 atgtgtgcaa cgtttgagaa cgtcctgagt ttcttggtca gatagtaact gattgattct
     7081 ccgcattttt ctgaacacaa aaatccctcc cgtaggttat tttaatctgt aagtgcgata
     7141 acctttatat tcaattatag caagagaggc atcggtttca agattttcca taaatttttc
     7201 aggaaaagaa tttccgcggg atttaagaaa ttcttcaatt tcaaattgat tcataggatg
     7261 acgttttatt atacttacaa atgcttcgta atcgtttttt atctcactat aaaatccgtg
     7321 tgagcttaag agatcaattg aaaacccatt caatatttca gcaatatgct ccattttttc
     7381 atgatccaat gcaaatacat caatttctgc aggaggacga acaggcgtat ttaagtaaag
     7441 cttttcatat ttaatttttt taagaatctc tgaatatttt ataattgtat tagggtcatc
     7501 attaatacct ttcataaaca ttatttcaag ccataactgt cccttatatt ttctggaaaa
     7561 atcttcaaga ccgttaagaa ctttataata attaaggctt gaatagggtc tgtttatttt
     7621 tttaaaagac ttttcatcat aagcatcaag tgtgggaaga agtatatcgc tctgcattaa
     7681 gtcagattga acatcagggt cgtatagaag agagccgttg gttattactg ctattggttt
     7741 attagttaat ttatgtaatt tttcaatgag ttgccctaga cctgagtata atgttggttc
     7801 accttctccg actattgtta taacatcata gtttatattt ttctggaaaa atgttttcaa
     7861 ctctgcaata atattatcaa ctggataaaa aagctgtcta tgattaatca tccgggttgt
     7921 tcttcccagt tggcaatata tgcaggagta gttacatgtt ttcttaggta taggactaac
     7981 ccctagagat attccaagtc ttcttgaagg aactggtccg taaacatact taaactcatc
     8041 cataattaaa cactccctta aaggatatat gtcataatat gcctatattg gtgatataaa
     8101 agaaatatag aggatttaaa tcctctatat ttcttttata tcaaacgtat agtattttct
     8161 tacaggttcc ttgatactcc aatgacattt cgcgcctttt tctatttgaa caagatcgcc
     8221 tgcttctact tctaccttat gagtcttaaa ttcaagtaaa gctcttcctt ctaaaacaaa
     8281 aaatgtttca gttgaatcgt atatatgatc aaactctgaa atatcttttt cccatatttc
     8341 ccattccttt actgcaagcg aacgaagacg ttcttccata ggaaactcaa ccactgtaac
     8401 tcccggatat gatttcactt tttctttagg accttcaagg aatgcattca ttggattttc
     8461 cataacgcca tccattgaac ccagagtttg tcccaacatt tcgtagtaag catcaatatc
     8521 tgtctgatca aaattttctt taagaaaaag gtcaagcttg tcaaaatcag catcgggatt
     8581 ttttttgaaa aagctatcca ctgcatttat taaagagttc aattttattt cattcataac
     8641 tgcaacctca taatctcctt atatgcctct ataactttgt tggttacttc tgttgtaagc
     8701 tttaatgaaa gcgaagcttt ttcagctgca acagtaacct gctcaatact agttatacgc
     8761 cctgccgcat agtctgcagt catcttatcg gatttaactt gaagatcatt aacttcattg
     8821 atggcatctt taagcatttg agagaaatta agatcgttct gaccagtagt ttttttgttt
     8881 ttttgtattt caagattgtg aagtccttta agaccgttta ttctttcgat catcactttt
     8941 caccccctat tgtcttccta tattcaaagc agaattaaac attgaacgtg cagttgaaat
     9001 tacagcagtg ttagcatcat atgccctttg agcagtcatc atatcaacca tttctttaag
     9061 cgctacaaca tttgggtatc gaacataacc atcagcatcg gcatcttgat gtgtaggatc
     9121 atacacaagc ctgaaaggag cgggatcttc ctcaatagaa tcgacctgaa cacctgaaaa
     9181 actgtttgcg tcatcgtcgt tgaacctact atcgagtatt tcacggaagg taacgtactt
     9241 tcgcacgtac ggaccgccat cttcggttct tgtggtttcg gcattggcta tattttgagc
     9301 aataacatta aaccgcattc tttcggccgt catgccgctt gctgctacat tcatagatct
     9361 aaagaaggat tccatttatc atcgcactcc ttttatgaca gtattatacc gctgaatcga
     9421 atagttcata agacgggtaa gtccgttata gtacataccg tttatataca tatttactga
     9481 ttcgacatct acgttaacgt tatttccatc ttctctgttt gatctggaag tatctttcaa
     9541 aatctgtggt tgaacttgcg tcaaagatgg tgtattattt atatgtgagg gtttgttggt
     9601 cttgagagct atctcgtcag aacttccaag ggctttctga aactcttcct caaaggtaac
     9661 gtactttctt ttatagtctg gagtgctctg attagctata ttctgcgcta taacagaatt
     9721 tctgactgat gccgcattca tggcctgcgg aagtatcgaa aaagttaagt caaacatgtc
     9781 tatccctcct aactgatatt tccatattca aaatagattt tcctaaaaaa gtcggaggtt
     9841 taaatgggaa aaatatttct tgtaggatac tatgggtaca ataactttgg tgatgagctt
     9901 ttgctcaaca gtatgctcaa aattttaaat gatgtgaaat ttcagggaga ggtaatcatt
     9961 ccttctgata atctcttgaa aatagaaact ccaataagct acaaagtaac tgatataaaa
    10021 aaatttgatc tttataatat tgttctttca ataaagagca gcgacttggt cattttcgga
    10081 ggaggcaacc ttttccaaac agaaacatct cttaagagtt ttctttatta ctattatata
    10141 gcaaaaaaag ctattaagta caagaaacct ctatttctta tttcccaagg atttggacct
    10201 ataaaaaata aatttgccaa taagcttctg aagtctcttt taaaaaccga gaatgcacaa
    10261 atatatgggt tttacaggga tgaggcatct tttagatatg ctcggagata ctgtaaaagt
    10321 tcttttaaca tagtcgatat cgctccctat gcaataagcg atataggact gcgggctcag
    10381 accacagata taacgagtat ctgtataaaa aaagattacg atgactttat gccgctatat
    10441 gaatttttgg aaatagataa tagaagtata aatactctgg caatcaattc caaccaggac
    10501 tctgagatat ccttcaaatt tgtggattat ctcagaagtg tatctgactg gagtgtgcct
    10561 ttcccaaccg gagacaataa aaagatccaa tctactttga taaactccaa gcttgtaatt
    10621 tctgacaggc ttcatagtgc tgttgtacct atgtattttg gtgtacctac agtagtcagt
    10681 gccagtgcaa aaaacagaag agttgtaaaa aatatctgtc ctgattacga ctacttttat
    10741 aaaaagcttt cggatattcc aggagcctat gcatatatga aagcccgaaa ttacgatcct
    10801 caaaaaaccg cagcagttta cagagaaaag ctttctgaag cagttcagac gatagagcaa
    10861 aacctcagaa ctttcttata acctgtacct tccctgcttg agtagaaagg acgatgatta
    10921 cactttcatc aggaacctcg acatcaatgg gataatcatc aagttttatt tcctgatata
    10981 cttggaagtc ctttattata aagagcttat tttctatact tgaaagtgca aaaatatagt
    11041 ttttggaggg agaaacagct atgtcaacaa taggtatgtc ctcaagcatt cgtacaattc
    11101 cttttttttc tatatcgtag aagtatatcc ctccaaaaag agagttgcct actatataac
    11161 tgttgaaaag ctcaatgttt ataagatatc caccaaaagc cgccacttct ttctgtccgg
    11221 caacaaggtt ttcatagatc ttaccgttgt agtatgaagc tatgaaaagt ctattattgt
    11281 attccagaat atctataggc gaacgtatat tctgatagtt tactatataa gagcctaccg
    11341 ttgcattgta aaaaagaaat cttgttgtta ttgtattgtc tttggtgttt tctctcatcg
    11401 gtatatacag atcttttccg gcaatttgaa gaaaagggct cttaactgaa aggtcgatgc
    11461 tctggataag tgaaaggttc ttatcaaata caaataattt gtcagggtca aaagcagata
    11521 taaaaatttt attatcaata gttttcatgt taacggggtt agatccaaga gagaccctct
    11581 ttattacggt gtttcctcgt agtttgagaa cttctttcct gtaattgtct ac
//
LOCUS       DAOSHR010000050        11592 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_4672_length_11592_cov_6.346451, whole genome shotgun sequence.
ACCESSION   DAOSHR010000050 DAOSHR010000000
VERSION     DAOSHR010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 11592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11592
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4672_length_11592_cov_6.346451"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(158..1576)
                     /locus_tag="PLU28_04135"
     CDS             complement(158..1576)
                     /locus_tag="PLU28_04135"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOG34288.1"
                     /translation="MKFFKTDKKNKQLSIFLEKNGMSDTMGFIGELEESIPQVKDSVK
                     HAISAAGRMSGFDLKMKQYSRVLKLQAQNIKDISLKTDEFIKEIEQAMNQISQASVNN
                     TSHISVLSEEISTMKNYISDSYSNLGKIKQENEKVSEKGTELSKQMNEFSELVEKMRE
                     LIKGIEDIAGQTNLLSLNASIEAARAGEAGKGFAVVADEIRKLSDDVKNNVGDMRDFM
                     DRMEDTSGRNLEVISQTVKSIEQITHNTNEINKVFEQSTKLIRSISEEAGYVVSNSEE
                     TTASTEEISSEIGVLKELINDASKASVELENNSEELSEISTEAAKIEEDISSLARKSG
                     KIASYKLFHLDIKDYAQSIDNAITAHKNWLKILRKIKDEMKVLPLQLDDKKCSFGHFY
                     HSVQVNDPQVIEIWKSIDLVHQNLHTKGSQVVKAVELHDKAQADLLYNETEKVSRQVL
                     EKLEKVLNAIKQTDKTNLTDNR"
     gene            complement(1642..2571)
                     /locus_tag="PLU28_04140"
     CDS             complement(1642..2571)
                     /locus_tag="PLU28_04140"
                     /inference="COORDINATES: protein motif:HMM:NF020476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HOG34289.1"
                     /translation="MIISVSRRTDIPAYFPQWFIKRVEEGFVYVRNPFSYHQVSKVLL
                     GSGDVDCFVFWTKDPLNLIDHLKEIKNYSYYFQFTVTPYGKDIEPGIRDKSNIIKTFK
                     ELSLLIGKEKVVWRYDPVIFTEKMDIEYHIHEFEWLMDEIGAYCAKLVISFFDSYKGI
                     EKRTSGNKIINISEKDIKVLARSFSKKAGKIGLKLETCAEKIDLSEFGIEHTSCVDAK
                     IISRITGKNFNTLKDNNQREFCGCIPSIDIGSYDSCPAGCIYCYANHSEKKLRYNISR
                     HSYSSPLLIGEVLAEDKIYEKTQLKNRQTRLKF"
     gene            complement(2568..3221)
                     /locus_tag="PLU28_04145"
     CDS             complement(2568..3221)
                     /locus_tag="PLU28_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34290.1"
                     /translation="MEIKTVREAADELLKKIQQYKELWKKLEDNVSLSDKKMDAITRA
                     EKTALTYFEEAVDKAKDEVQALRSEIEKENSFFFKKNIEMVEVVSNIENRIEEIKKVC
                     DSIKIKEFKDNLSKEVQMLSDEISDRYAEMQISISEAFKASHESKILIQNSTEGLFRS
                     TESFKAETIQVLVNTQEETEKKFNKIEHKLKNYRIVTIALALVNILFSIWLYFKSGL"
     gene            complement(3190..6018)
                     /locus_tag="PLU28_04150"
     CDS             complement(3190..6018)
                     /locus_tag="PLU28_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34291.1"
                     /translation="MWKNEKINTLLVHKRAWKSIKSRKVSEEKLKQDIDDAVNELFSP
                     NSSKNIKMISSGFFRKRLSYSKRMIFDYDYEKKFFLVYDIGNHDVYDKICEYRDLPKN
                     LGNSFLEIAGDQFLEIKNNEKSLELIDTPDIYFFLDKVQQKIFDQRYKYSSVNFFSKT
                     QVFKGYPGSGKTSLLSRMAFDFLKDGKEIVYITYNYELKDFLKKTLLAFNDEINREGL
                     EHSKMEKKINNSRIYTYEEFILKEICLYIGINTSKYILYRESQEILEKLKSNKMDRTF
                     QKIFEDVNMPMNELTRFVHAFCYTQANESNIQEYISKKLDRSGIVSYDRERLAMIKIL
                     QEYQRALDVKKKIRWYLLFEEIKEKRPMLKDKSFPAILLDEMQDLDQTEFNMLKEIFK
                     ICKKDDFLFKVAYDPNQNIEVHGSGSKNIENRLKEIDKSQKYELRYNYRNSQNIKIFS
                     SYFINAGTELYDEFMHGNESFVQVKLTVCSDKQALEQLQKIVEIKNISLPILAYGDLC
                     QELKKNVEGKEERMIFFNENQIKGMEFSNLVILNFLSPYIKRNKKIESIDKQKWFVGV
                     SRAKENLLITFSTYEEYEVFLKEVTGKDMQKAGNCYCFFEDYLKAFESFETDYYEEES
                     FEKIKMVQKNFEEELEEIINGTKSVSEKFLNSATVLINNELIFSVYNLLCRLEFTNDP
                     RIAKLILLIVRKINGTSGKKEFNEYKNWIKILNSNDKSGLKEVYSYFLKQSMYEECQA
                     IAEYTDSDELFLDLCEDICLKYLEEKIDINWFDNKIDSLVQKIELNLSQSVDRKKAVF
                     LLMCEVLLKKGKKEEAVEYYYEYKEYRKIFDVFESSYSGDFKNEQSRAMILASFLNLN
                     FKAEQIVDCFCKLYGVKDKDIGKILERLQGLLDGIDQKSLRKADIGKLEELICSIDIS
                     MFLETITISNNILKEITADGNQNCKRGS"
     gene            6279..7016
                     /locus_tag="PLU28_04155"
     CDS             6279..7016
                     /locus_tag="PLU28_04155"
                     /inference="COORDINATES: protein motif:HMM:NF019322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOG34292.1"
                     /translation="MADELHSINDGKSVVSRTYEALLKYVNNPKRSGEKLPSEIELSK
                     QLKVSRTALRDALKKLELEGYISRKRKTGTFINCSNYKLHGGLEKLRSITELIKTSGM
                     RPGTIFSKYSTDNADNIISQKLGLADSSRVSIIERVRTADDTPFCYEISFVPISYFNQ
                     EDYENFSGSLLSYLSEKKGLVVDHAVTYLNPYSSDDLISEKLSIKNNQLIMYIEQVHY
                     SVDDEPIWYSRAFYRNDIIRFSLMRRL"
     gene            7027..7962
                     /locus_tag="PLU28_04160"
     CDS             7027..7962
                     /locus_tag="PLU28_04160"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HOG34293.1"
                     /translation="MKILGLGSIAVDRIFTVDRLPVKDGFSNILDEKVYDGGSCANVI
                     NQIAMYGGKSAFCAKIGSDDTSEVIMTGLKKSGIDTAYMIKKENGISTSTVIYVDQNG
                     DKSILTRVGDCLLNLSDEELNSEIFETFDILYTDFIPSRACLYAAREFKKRGKKVIFN
                     LQVAPVVMKGFGMTDEIFAELFKYLDIFNVSFSTLKDISGLSELEENIAFLRKTYNYK
                     GTIVLTMGSKGACICSADGQTIKQPAFKISPIDTTGAGDSFIGTFIFLHYFKNESMER
                     SLSFSNAAAAITCTRKGARSGPDWNELVSFMKEGF"
     gene            7963..9447
                     /locus_tag="PLU28_04165"
     CDS             7963..9447
                     /locus_tag="PLU28_04165"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOG34294.1"
                     /translation="MEQSILSVKNICKNFPGVRALDNVCFDLRQGEVHALLGENGAGK
                     STLMKIIFGLLKKDSGEIFLRNSELKSISPSKSYENGIALIPQERTLLTHKSIAENIF
                     LGMEPAFVSKKEMCLQSLEMMKRFSMQISPDTPAAQLSAGMQQTVEIMRAINRKAQIL
                     IMDEPTGGLSAQEVKRLFEVIRTLKGQKASVIYISHRMEEIFEISDRITVLRDGKNAG
                     TFGTADVTKEKIIELMAGKKISAQKQNKSSIGEEVLQVKELCSNKIHNINLTLRKGEI
                     LGIAGLLGSGRTEIFECIYGINRDYKGRIFINSKDCTHYSISDAISCGVGLVTEDRKR
                     TGLSLKSSVAKNITLPMEKMIFRRGIINTKKVQTISSEYSEKVGLNTSDMSFTSGNLS
                     GGNQQKVVIARWLANGSKILLLDEPTVGVDVNAKQEIHQLIRQYCNGENSVIVASSDF
                     PELLEICDRIVVIAQGKVVKELENKDIDEKILLGYATSAGGEKI"
     gene            9444..10391
                     /locus_tag="PLU28_04170"
     CDS             9444..10391
                     /locus_tag="PLU28_04170"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG34295.1"
                     /translation="MRKISLLKAPEFGVSIALVGLFILMSFLSPLFLSGYNLLNILLQ
                     SSTYIILSIGMTFVIVTGGIDLSVGSIAVVSGILMAGVMKGGTPVWIAIIAGLAISTL
                     CGFVNGFLVSKINLPSFIVTLATMSIFQGAALIFSGGLPIYGFPKSFSFLGTGSIFSI
                     PVPVIIAFICIAVSVWVFKKTRIGHYALAIGDNRQAVRMCGVNVFKYELIVYMINGLA
                     TGVAAIITTARLNSAEPLASMSMGLDAVAAVVMSGTSLSGGSGSVTGAIIGSLLMGVL
                     RNGMTLLNVQSYYQNLIIGVVILIAVFIDKIRNSYSYKK"
     gene            10410..11423
                     /locus_tag="PLU28_04175"
     CDS             10410..11423
                     /locus_tag="PLU28_04175"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOG34296.1"
                     /translation="MKKYLAFVLTFVMIFSIALSAQSAFERAKADVLKELGGAEKTSK
                     VYKIGALEITLANPFWVTMKEGFEAAAKDFGFKIDVVAAPSEGDTAAQLNYLETMLIK
                     GYDALCISPITPFNLIPAVAKATQRKIPVIAVGTYVDPQAAKDAGAKINGILAVDFEY
                     QGKLPGQYIIEKFKNTKNVKAAIIEGIPGSPQSEGRKNGCLNELKKASNIKVVSVQPG
                     NWDRTTALNVTSNLIQANPDLNVIFCANDTMALAAVEALKAVGKKKQVMVIGCDFIEE
                     AKESIIKGELDATLAMSPYLYGYIGGVMAYKTIIDGQYSKDIQVPIKIVDKSNLSEYN
                     DWR"
BASE COUNT         3608 a         2256 c         1978 g         3750 t
ORIGIN      
        1 tttgcgtgca gtatcggcta cactttcccg gccagatgtt aaaatcggat tttcgaccga
       61 aaaggcaccg gatcctccgc caaaaacata taaactgctt aaaattaaaa tcaacaaaaa
      121 taatgttttt tttatcatct ttaaaccctc ccaatgatta tctgttatct gtcaaatttg
      181 ttttatcagt ctgctttatg gcatttaaaa ctttttcaag tttctcaaga acctgcctgg
      241 atactttttc tgtttcatta tagagcaaat cagcctgtgc cttatcgtgc aactccactg
      301 cctttacaac ctgtgaacct tttgtgtgca gattttgatg gacaagatct attgacttcc
      361 atatctcaat gacttgcgga tcattaacct gtaccgaatg ataaaaatgt ccaaagctgc
      421 actttttatc gtccagttga agcggcagca ccttcatctc atcttttatt ttccgcagga
      481 ttttaagcca atttttatga gcggttatgg cgttatctat gctctgggca taatccttta
      541 tatcaaggtg gaaaagtttg taagatgcta tctttccact ttttctggct aatgatgata
      601 tatcttcttc gattttggca gcttctgtgg aaatttcaga taactcctca ctgttgtttt
      661 ccaattccac actagcttta gaagcatcgt ttataagctc tttcagcaca ccaatttcag
      721 agcttatttc ctctgtagaa gcagttgttt cttcagaatt tgaaaccaca taaccggctt
      781 cctccgatat actacgaata agctttgtgc tctgctcaaa tactttatta atctcatttg
      841 tattatgcgt tatctgctct atacttttta ctgtctgtga aatcacctca agattcctgc
      901 cagaggtatc ttccattctg tccataaaat ccctcatatc gcccacgtta ttcttgacat
      961 catccgaaag cttgcggatt tcatcagcca ccactgcaaa acctttcccg gcttctcctg
     1021 cacgtgccgc ttctattgat gcgttcagag acaagaggtt cgtctgacca gcaatgtctt
     1081 caattccttt aatgagttcg cgcatctttt caacaagctc agaaaattca ttcatctgct
     1141 ttgagagctc tgttcctttt tcagagactt tttcattttc ttgttttatt ttgcccagat
     1201 tactataaga atcagaaata taatttttca tagtgctgat ctcttccgac aggacgctta
     1261 tgtggctggt attatttaca gaagcctgag agatctgatt catagcctgt tctatttctt
     1321 ttatgaactc atcagtcttt aaggatatat ctttgatgtt ttgagcctgt aactttaata
     1381 cccgactgta ctgcttcatc ttaagatcga atccgctcat ccttccggct gctgatatgg
     1441 catgctttac cgaatccttt acttgaggaa tactttcttc aagttctccg ataaatccca
     1501 tagtatcaga cataccgttt ttttcaagaa aaatagaaag ctgcttattt tttttatcag
     1561 ttttaaaaaa tttcatagcc tcctcctttt atacgccttt acttaatgat accattatga
     1621 aacagatcat acaaacactt tttaaaattt taatctggtt tgcctatttt ttaactgagt
     1681 tttttcataa attttatctt ctgcaagtac ttccccaata agcaaaggcg agctataact
     1741 gtgccggctg atgttgtagc gtagtttttt ctccgaatga ttcgcataac aataaataca
     1801 acccgcagga caagaatcat aacttcctat atctatactt ggaatacaac cacaaaactc
     1861 cctttgatta ttatctttca aagtattgaa gttcttgccg gtgatccttg aaataatttt
     1921 tgcatcaaca cagcttgtgt gttctattcc aaattctgac agatcaattt tttcagcaca
     1981 tgtttctaac ttcagaccta tctttcctgc tttttttgaa aaactccgag ccaatacttt
     2041 tatgtctttt tcagatatat tgataatttt gtttccagaa gttcttttct ctatcccttt
     2101 atatgaatca aaaaaactta tgaccagctt tgcacaatat gctccaatct cgtccataag
     2161 ccactcaaac tcatgaatat gatactcgat atccattttt tccgtaaaaa ttacagggtc
     2221 gtacctccat accacctttt cttttccgat aagcaatgaa agctctttaa aagtttttat
     2281 tatattgctt ttatccctta tgcccggttc aatatcctta ccgtacggag taacggtaaa
     2341 ctgaaaataa taagagtaat tttttatttc tttaagatga tctataaggt tcaacggatc
     2401 cttggtccag aaaacaaagc aatcaacatc tccgctgcca agcaatactt tgcttacctg
     2461 atggtaagaa aatgggtttc tgacatacac aaatccttct tcaactcttt ttataaacca
     2521 ttgaggaaag tatgcgggga tatctgtccg tctgctcacg cttataatca taaacctgat
     2581 ttaaagtata accaaatgga aaatagtatg tttaccaaag ccagagcaat agtaactatt
     2641 cgataatttt tcaacttgtg ttctatttta ttgaacttct tttccgtctc ttcttgagta
     2701 tttaccagta cttgtatggt ttcagcctta aagctttctg tagatctgaa aagtccttct
     2761 gtgctgttct gaataaggat cttagattca tgtgatgcct taaaagcttc agaaattgat
     2821 atttgcatct ctgcatatct atccgaaatt tcatcactga gcatttgaac ttctttactc
     2881 agattgtctt taaactcttt aattttaatg ctatcgcata cttttttgat ctcctcgatt
     2941 ctgttctcaa tatttgaaac tacttcaacc atctctatat tttttttaaa gaaaaaactg
     3001 ttttcttttt caatctcaga cctcaacgct tgaacttcat ccttagcttt atccacagct
     3061 tcttcaaagt aagtaagagc agttttttca gctctagtta tcgcatccat ttttttgtca
     3121 gataaactaa cattatcttc taattttttc cataattctt tatattgctg aatcttcttt
     3181 aaaagttcgt cagctgcctc tcttacagtt ttgatttcca tctgcggtta tctcctttaa
     3241 aatattattt gatatagtta ttgtttccaa aaacatactt atatctatag aacaaattag
     3301 ctcttctagc tttccaatat cggcttttct caaacttttt tggtcgatcc cgtctaaaag
     3361 tccctgtaaa cgctctagga ttttaccaat atctttatcc tttactccat acaacttaca
     3421 aaagcaatct acaatctgtt ccgctttaaa attaaggttt aaaaaggatg caagtatcat
     3481 tgccctagac tgttcatttt taaaatctcc ggaatacgaa ctctcaaaaa catcgaaaat
     3541 ttttctatac tctttatact cataatagta ctcaactgcc tcttcctttt ttcctttttt
     3601 caataaaacc tcacacataa gcaaaaatac ggcctttttt ctatccaccg attgagaaag
     3661 gtttaactca attttttgaa ccaaactatc aattttatta tcaaaccagt ttatgtctat
     3721 tttttcttct aagtacttca agcatatgtc ttcgcacaga tcaaggaaaa gctcatcgct
     3781 atcagtatac tcggctatgg cttgacattc ttcatacatg ctttgcttaa gaaagtatga
     3841 ataaacttct ttaagtccgg acttgtcatt gctattgaga attttaatcc agtttttata
     3901 ctcattgaac tcttttttac ccgaagtacc gtttatcttg cgcactatta aaagaataag
     3961 ctttgctatt cttggatcat ttgtaaactc caatcggcaa agcagattat atacagaaaa
     4021 aattaactca ttattgataa gtactgtggc tgagttaaga aacttctcag atacagactt
     4081 ggtaccgtta ataatctctt ccaactcttc ttcgaagttt ttctgaacca tctttatttt
     4141 ttcaaaactc tcttcttcat aataatccgt ttcaaaactc tcaaaagctt ttaaataatc
     4201 ttcaaaaaaa caataacagt ttccagcttt ttgcatatcc tttcctgtta cttcttttaa
     4261 aaaaacctca tactcttcat atgtactgaa ggtgatcaaa agattttctt tcgctcggga
     4321 tactccgaca aaccactttt gcttatctat actctctatc tttttgtttc tttttatata
     4381 aggagataaa aaattaagaa ttacaaggtt tgagaactcc attcctttta tctgattttc
     4441 attaaaaaaa atcattcttt cttcttttcc ttcaacattt tttttaagtt cctggcataa
     4501 atcaccatat gccagtatag gaagacttat attttttatc tcaacaattt tttgaagctg
     4561 ctctaaagct tgtttatctg agcaaaccgt aagttttact tggacaaatg attcgttacc
     4621 atgcataaac tcatcataaa gttcggttcc ggcatttatg aaataagaac taaatatctt
     4681 gatattctgg gagtttcgat agttatacct tagctcgtac ttttgagatt tatctatttc
     4741 ttttaaacgg ttttcaatat ttttagatcc cgatccatgg acttctatat tttgattggg
     4801 atcatatgca actttaaaaa gaaaatcatc ttttttacag attttaaaaa tttccttcag
     4861 catattaaac tcagtttgat ctaaatcctg catctcatcc aacaaaatcg ccggaaaact
     4921 cttatcttta agcataggac gcttttcttt tatttcttcg aacaaaaggt accacctgat
     4981 tttcttttta acatccaagg ctctttgata ctcctgaaga atttttatca tagcaagcct
     5041 ctcacgatca taacttacaa ttccgcttct atcaagcttt ttagaaatat attcctgtat
     5101 gttgctttca ttggcttgag tatagcaaaa agcgtgaaca aaccttgtaa gctcgttcat
     5161 aggcatattc acatcctcaa aaattttctg aaacgtacga tccattttgt ttgattttaa
     5221 cttttccagt atctcttgac tttctctgta aagtatgtac tttgaagtgt ttatccctat
     5281 atatagacag atttctttta atataaactc ttcatacgta tagatccttg aattattgat
     5341 tttcttctcc atttttgaat gttcgagacc ttcacggtta atctcatcat taaaggctaa
     5401 aagagtcttt tttaaaaagt ctttcaattc gtagttatag gtaatatata ctatctcttt
     5461 cccgtctttc aaaaaatcaa atgccattct gcttaaaaga gaggttttac cacttccggg
     5521 atagccttta aaaacttggg tttttgaaaa aaagtttaca ctcgagtatt tatatctctg
     5581 atcaaagatc ttttgttgga ctttatcaag aaaaaaatat atatcaggtg tatcaattag
     5641 ctctaaagat ttttcattat ttttgatctc caaaaactga tctccagcta tttctaaaaa
     5701 agagtttcct aaatttttag gtagatcacg gtactcacaa attttgtcat atacatcatg
     5761 attgccaata tcgtatacca agaaaaattt tttttcataa tcgtaatcaa atatcatcct
     5821 ttttgagtag ctaagtctct ttctaaaaaa tccggagctt atcatcttta tattctttga
     5881 actgttaggg ctaaatagtt cattcactgc atcatcaata tcttgtttga gtttctcttc
     5941 cgatactttt ctgcttttta tacttttcca tgctctttta tgaaccaata aagtgtttat
     6001 tttttcattt ttccacattt atcctcccaa aaaaataatc cattcaaatt taaattatta
     6061 tctatttcta ttataccaaa aatacttttt taattgaggt ataaaaataa tttctattag
     6121 taatacctat tactaataat tggatttttt tatataaagt caaaaaaacg gtttttactg
     6181 tttataagtc cagataactg aattacattt ttagaaaaaa ataaattata atgctaatgg
     6241 taataccata tactaaaacc tataaggagg catgaagtat ggcggacgaa ctgcatagca
     6301 ttaatgacgg gaaatctgta gtttcaagaa catacgaggc cttgctaaag tacgtaaaca
     6361 atccgaaacg ttcgggtgaa aagctccctt cggagattga gctttcaaaa cagcttaagg
     6421 tgagcagaac tgcgttacgt gacgcactga aaaaacttga gcttgagggg tatatttcaa
     6481 gaaaacggaa aacaggaacc tttattaact gcagcaacta caagctccat ggcggactgg
     6541 aaaaacttcg ctccataaca gagcttataa aaacttctgg aatgcgtccg ggaacaatat
     6601 tttccaagta ttctacagat aatgcagata atattatttc acaaaagctt ggactcgccg
     6661 acagttccag agtatctata atagaaaggg tgagaactgc tgacgatacg cctttctgtt
     6721 atgagataag ctttgttcct ataagctact ttaaccagga agattacgaa aacttttccg
     6781 gttctctcct gtcttatcta agtgagaaaa aagggcttgt agtcgaccat gcggtaactt
     6841 atctcaatcc atactcttcg gatgatctta tcagtgaaaa actttcaata aaaaacaatc
     6901 agctcataat gtatatagag caggtacatt actcggtaga tgatgaacca atatggtatt
     6961 caagagcttt ctacagaaac gatataatac gtttttctct gatgagaaga ttataggagg
     7021 taagacatga aaatcttagg attaggaagc attgcagttg acagaatttt cacggtggac
     7081 aggcttcctg tgaaagatgg tttttcaaat atactggatg aaaaagtcta tgacggagga
     7141 agctgcgcca acgttataaa ccagattgcc atgtatggcg gcaaaagtgc tttttgtgcg
     7201 aagatcggaa gtgacgacac atcagaggta attatgaccg gacttaaaaa aagcggaatc
     7261 gatactgcat atatgataaa aaaagaaaac gggatctcaa cctccaccgt tatttatgta
     7321 gatcaaaacg gtgataaatc gattcttaca agggtcggtg actgtctgtt aaatctttcg
     7381 gatgaagaac tgaactctga aatatttgaa acgtttgaca tactttatac agacttcatt
     7441 cccagcagag catgtcttta cgccgccagg gagttcaaaa aaagaggaaa aaaagttatt
     7501 ttcaaccttc aagtcgctcc tgttgtcatg aaaggctttg gcatgactga tgaaatattt
     7561 gccgagcttt tcaagtatct ggatattttc aacgtatctt tttcaactct gaaggatata
     7621 agcggtcttt ccgagctgga agaaaacatt gcttttctga ggaaaacata caattataaa
     7681 ggcaccatag tgcttacaat gggaagcaag ggagcatgta tatgctctgc tgatgggcaa
     7741 acaatcaaac agccggcttt caaaatatca ccaatagata ctaccggagc aggcgactct
     7801 tttataggaa ccttcatttt cttacactac tttaaaaatg aaagcatgga aagatcgcta
     7861 agcttttcaa acgcagctgc cgccattacc tgtacaagaa aaggagcaag gagcggaccg
     7921 gattggaatg aactggtatc ttttatgaaa gaaggttttt agatggagca aagcattcta
     7981 tctgtaaaaa atatctgtaa aaattttcct ggagtcagag ccttggataa tgtatgcttt
     8041 gatcttcgtc agggagaggt tcatgctctt ctgggcgaaa acggtgcggg aaaatccaca
     8101 cttatgaaga ttatttttgg tcttctgaag aaagacagcg gagaaatttt tctcagaaac
     8161 agtgaactta agtccatttc tccttcaaag tcttacgaaa acggaatagc tctaatacct
     8221 caggaacgga ctctccttac ccacaagagc attgccgaaa atatattcct tggaatggag
     8281 ccggcttttg tttcaaaaaa agagatgtgc cttcaaagct tggaaatgat gaaaagattt
     8341 tcaatgcaga taagtcccga tacccccgca gcacaactaa gcgcaggtat gcagcaaact
     8401 gtcgaaataa tgagggctat taacagaaag gcacagattc tgataatgga tgagcctaca
     8461 ggaggtctga gcgcccagga agtaaaaaga ctttttgaag tcatcagaac tctaaaaggt
     8521 caaaaggcat cggtaattta tatttcacac agaatggagg agatttttga gatctctgac
     8581 agaataactg tgctgcgcga tggaaaaaat gcaggaacat tcggcacagc agacgtgaca
     8641 aaggaaaaaa taatagaact gatggcagga aagaaaattt cggctcaaaa gcagaataaa
     8701 agcagtatag gcgaagaggt tctccaagta aaagagcttt gcagcaataa aatccataac
     8761 ataaacctta ctctgcgaaa gggagaaata cttgggatcg ccggacttct gggaagcggc
     8821 agaacagaga tttttgaatg tatctatggt ataaacagag attacaaggg cagaatattt
     8881 atcaactcaa aagactgcac ccattattct atatccgatg ctatctcatg tggcgtagga
     8941 cttgtaacag aggacagaaa aagaacggga ctttctctta aatcctcggt tgcaaaaaat
     9001 ataacccttc ccatggaaaa gatgattttc cgccgtggaa tcataaatac aaaaaaagtc
     9061 cagaccatat cttcagagta ctctgaaaag gttggactca acacaagcga tatgtccttc
     9121 acaagcggta atctgagcgg cggaaaccag caaaaagtag ttattgcaag atggctggct
     9181 aatggatcaa aaattctcct gcttgatgaa ccaactgttg gagtggatgt aaatgccaaa
     9241 caagaaatac atcagctgat caggcagtac tgtaacggag aaaattcggt aatagtagca
     9301 tcttcagatt ttccggagct tttagaaata tgtgaccgta tagttgtaat agctcaagga
     9361 aaagtagtta aagaacttga aaataaagac attgacgaaa aaattttact tggatatgca
     9421 acttctgcag gaggggaaaa gatatgagaa agatcagttt gttaaaagca ccggagtttg
     9481 gcgtaagcat agcccttgtc ggacttttta tacttatgtc atttctgtcg ccattatttt
     9541 tatcgggata caaccttctc aacatcctgc tgcagtcatc aacatacata atactttcaa
     9601 taggaatgac ctttgttatt gttacaggag gaatagatct ttcggtaggc tcaatcgcgg
     9661 tggtaagcgg aatattgatg gcaggagtta tgaaaggcgg aacaccggtc tggattgcca
     9721 tcatagcggg acttgcaata agcactctat gcggctttgt aaacggcttt cttgtttcaa
     9781 agatcaatct tccttccttt atagtgacct tggcaaccat gagtattttc cagggagcag
     9841 ccctgatatt ttcaggcgga ctgcctatat acggttttcc taaaagtttt tcatttttgg
     9901 gtacaggatc aattttttca attcctgttc ctgtaatcat agcgttcata tgtatcgccg
     9961 tatctgtttg ggttttcaaa aaaacacgca taggccatta cgcactggca ataggggata
    10021 accgccaggc tgtaagaatg tgcggagtaa atgttttcaa atatgaactg atagtgtaca
    10081 tgataaacgg actggcaaca ggcgtggcag ccattataac gacagccagg ctgaacagtg
    10141 ccgaacctct tgcttcaatg agcatgggac ttgacgccgt agctgccgtg gtcatgagcg
    10201 gaacaagttt gtccggagga agtggatcgg taacgggagc cataatcgga tcacttctta
    10261 tgggagttct cagaaacggg atgacacttc ttaacgtaca gtcgtactat cagaacctca
    10321 taataggagt ggtaattctt atagcggtat tcatagataa aatcagaaac agctactcat
    10381 ataagaaata gctgttcagg agggatatta tgaagaagta tttggcattt gtactaactt
    10441 ttgtcatgat tttcagtata gctttatccg ctcagagtgc atttgaaaga gccaaagccg
    10501 atgttcttaa ggaactcgga ggcgcggaaa aaaccagcaa ggtctacaaa ataggagctt
    10561 tggaaattac tttggcaaac cctttctggg taactatgaa agaaggtttt gaggcagcag
    10621 caaaagattt cggctttaaa atagatgtcg ttgccgctcc atccgaaggc gataccgccg
    10681 cacagctcaa ctatcttgaa accatgctta taaaaggata cgatgctctg tgcatctcac
    10741 cgattactcc gttcaacctt atacctgcag ttgccaaggc aactcagaga aaaataccgg
    10801 taatagcagt tggaacatat gttgacccac aggctgcaaa agatgcaggc gcaaaaatta
    10861 acggcatact tgcagttgac tttgagtatc agggaaaact tccgggacag tacataatag
    10921 aaaagtttaa gaacacgaaa aatgtaaaag cagcaataat agaaggtata cctggttcac
    10981 cacagtctga gggaagaaaa aacggatgtc tgaatgaact taaaaaagct tcaaatatta
    11041 aagttgtatc tgttcagccc ggaaactggg atagaactac cgcccttaac gtaacatcaa
    11101 acctcataca ggctaatcct gatcttaacg ttatcttctg tgcaaacgat acaatggctc
    11161 tggctgctgt agaagcactt aaggccgtag gaaagaaaaa acaggtaatg gttatcggct
    11221 gtgatttcat cgaagaagcc aaagaatcca ttattaaagg cgaactggat gccactcttg
    11281 caatgtcacc ttatttatat ggatacattg gaggcgtcat ggcctataaa accataatcg
    11341 atggacagta ctcaaaagat attcaggtac ctataaaaat agtagacaaa tcaaatctta
    11401 gcgaatacaa tgactggaga taacacccgg gaggaaaaaa cagatgaact tatttgaaaa
    11461 ggttcttcag gcttttgcag ccggggacgc catgggtatg cccacagagt tcatgaccag
    11521 aaagcatatc aaaagcttcc tcaatgaagg cgaagatttt attgaccctt caaaatcttt
    11581 cttacacaag aa
//
LOCUS       DAOSHR010000051        11423 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_4780_length_11423_cov_5.099842, whole genome shotgun sequence.
ACCESSION   DAOSHR010000051 DAOSHR010000000
VERSION     DAOSHR010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 11423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11423
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4780_length_11423_cov_5.099842"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="PLU28_04180"
     CDS             <1..499
                     /locus_tag="PLU28_04180"
                     /inference="COORDINATES: protein motif:HMM:NF024045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HOG34297.1"
                     /translation="RSKKAIEQSEMIIFVIDGETGFTNEDKELYDFIVAQGKDNVILA
                     INKKDSVSFIKCEVPSFITWKTVEISALKNDISELENVMYSMLCDKVEINEPVLTNLR
                     QKQALMNSYEAMLAANSSYMQGYTSDVVMIDIRKAIQAINELIGADYTQDLLESIFSN
                     FCVGK"
     gene            518..1234
                     /locus_tag="PLU28_04185"
     CDS             518..1234
                     /locus_tag="PLU28_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34298.1"
                     /translation="MKKRIISILFGLIPFIQFFIGGWIFFGVSLILMIISYFIIRKNS
                     SLNFVVYALVLIASQIVVFMNGASNIPLFVYAAIASALFLVAASDEKVIDNLRKELNQ
                     TGVKTKEGVFELCFFGMGEINDLESLSGLKTTVVFIGDGRAAFTVPMPKSTISRTIML
                     DDIIDYGMFRLAKKQTPLYYTKISEMFLPAKRLRTLHKPEVETYGLYIDTADERLSFY
                     EEPKVLQKLENAIKRLKEKK"
     gene            1269..2423
                     /locus_tag="PLU28_04190"
     CDS             1269..2423
                     /locus_tag="PLU28_04190"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG34299.1"
                     /translation="MFELKKFFRNSGVISLIIILPVIIAYLGSVFYPENILANYKIAI
                     YNEDKTWLGQFGFIFLKQFFKWENAIEIKNEEALSTTIKSGDYDSIIIIPKGFMSDLK
                     EYKPTKLNLIPNPQKIQDSLAVYTVFKALFRELAGIPDLESGSTLQFLMSGGIGVDTQ
                     RKAPDIEILVPNMKTGSIRSEIKADIGFEDIFAPSVAVIMIMIFCMIGVGNSIAHTKE
                     SGLFDIYRSNGLKVYQFINFKFIAYFVVGLVASLFSWYIYRLFGVHSMAGEFEIIFLI
                     IVTSFSFTAFGILFSSFTKTSKSSGLLLTALTGCMMLFGDILIPIPQGSIIKNIVNFM
                     PIKYSVDAWRKVSVLGYSLPEVSYEILILIIFGVVSYLLSNIIMTRVEKK"
     gene            2462..3040
                     /locus_tag="PLU28_04195"
     CDS             2462..3040
                     /locus_tag="PLU28_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HOG34300.1"
                     /translation="MNIERSIFIASAVKVSSCQQAKDFIKEISRHYNDATHNCWAYKI
                     IENNSFQSDFSDDREPSGTAGRPILGAIEKNGIINVAVVVTRYFGGVKLGVRGLIQAY
                     AQSAQSAIDLAGVVLFKKFFLYSFKTDYSSFAQLEKILKREKGWAFKQKKFLDSIHAD
                     ILIKEDSLEKILSVLNTKVFDLEYLGEEEAEE"
     gene            3044..4438
                     /gene="dnaX"
                     /locus_tag="PLU28_04200"
     CDS             3044..4438
                     /gene="dnaX"
                     /locus_tag="PLU28_04200"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOG34301.1"
                     /translation="MVSLYRKYRSKTFDELIGQDHVRRYFANALDRKEISHAYIFTGP
                     RGTGKTSTARLLAKVLNCCDPDGYNPCNNCDNCRAIDEGSFMDVIELDAASNRGIDEI
                     REIRDSTNYRPVHGKYKVYIIDEFHMLTKEAFNALLKTLEEPPEHVIFILATTNLEKV
                     PQTIISRAQLINFKNIREKDIVMLLKRVSAQEGVTIDEKSLSIISKRAKGGARDALSL
                     FEQVMKFCSKEVTSFETLSILGLFDDNMVEEFINFLISGDVDNLLRLSSDLFESGKEP
                     EALIEQSIEKLFIKIKNDKNYALVKLVRELNEVSKELKYSENKKAVFEIYVTSLAYGY
                     KKLDIKYETNLINESINSLEQSFFTEDSEQTCGGDYQLMLQKLANESSKMNLGLFFAL
                     KNNTYEISEGKLTIFFPSESSMEYQITRKYLSELKINVLGTFKGVSEVKLVFDGDVKK
                     DFPSNQDLSSKLFS"
     gene            complement(4464..4763)
                     /locus_tag="PLU28_04205"
     CDS             complement(4464..4763)
                     /locus_tag="PLU28_04205"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOG34302.1"
                     /translation="MRKDIKGDTVRVYLEGSLDISNSKALEAELNDIKTKNYKKVIFD
                     MIGLNYVDSSGIGKIFYFFTSYKKTGGDVEIQNVKSPKVREVIQLIKMDKIIKIS"
     gene            complement(4760..5704)
                     /locus_tag="PLU28_04210"
     CDS             complement(4760..5704)
                     /locus_tag="PLU28_04210"
                     /inference="COORDINATES: protein motif:HMM:TIGR03830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system MqsA family
                     antitoxin"
                     /protein_id="HOG34303.1"
                     /translation="MDSNIFCDRCNKYVEAIKKEETIPMTIKGEIILFNGVRTFCPVC
                     KSNLYVESIHSENMEKGLQLYRKKHDLVSPLEIKEVRKLYDISQSLFSKILGIGEATI
                     RRYETGMIPSKVNSDTIKQARSPKAFMNFVEQNKENISPEDYEKVSAAVKNLLSDMDN
                     KKQKSEIQQIHKSYDMQKIFYIIHHIIASSSENDFIKILKLMWLIDYFYYKKNYRYFF
                     SSQPEEKLPYKHIFEEKLLFIVAFLAKNKEFEVKMLENDDGVLEILIKSTDADPNYRI
                     FEEDLSFVNQILSEYSGKSNSELKKIMTDLINQEGVIR"
     gene            complement(5832..7151)
                     /locus_tag="PLU28_04215"
     CDS             complement(5832..7151)
                     /locus_tag="PLU28_04215"
                     /inference="COORDINATES: protein motif:HMM:NF015515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HOG34304.1"
                     /translation="MDLFIGIAAAVFLLILSVVKNIYLVYPLILIDLIFIGIFLRRGF
                     KLKYLLSQAFKGGKKAFVVLQIFVLIGAITAVWMSSGTVPAIVFYGMRLLNPYIFIFS
                     CFVLSAFVSMLIGTSFGTASTIGIALIIMARSGGISPELAAGAIISGALYGDRCSPMS
                     SSANLVATLTGTDLFRNIKNMFKTAWLATLITLGIYMIFSVFNPLNTSDSTIVFHIAK
                     TFRTDLWVLLPAFIIIILSFFRVNVKLSMLVSIVAGFLISIFFQGYSFFEVLEFSVGG
                     FFLEKDNPLFKIISGGGILSMLKVALIVFFSSTITGLFEGTDAFSFIDKYMERVKTRS
                     GTLFSTIIVSIVSAVFGCTQVMAIILTEQIVKKTYKKKNFDSYELAVDIENTAVVLSP
                     LIPWNIAGLVPATTLGVTSGFIPFAFYLLVLPVVYYLNLWKKDRNIA"
     gene            complement(7164..8267)
                     /locus_tag="PLU28_04220"
     CDS             complement(7164..8267)
                     /locus_tag="PLU28_04220"
                     /inference="COORDINATES: protein motif:HMM:NF019585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HOG34305.1"
                     /translation="METIIVTHSEEKNGIFLSYIENSPFYPDGKGGQLGDRGMIENIP
                     VLACYEKYVQTERFVEPGEHKYSISSERKNDICQQHTAQHIISAAFENVHNIQTVGFR
                     MDENYSTVDLDVPFITQELTDEMEELSNKIVSDAVQVEEILTDIEGSKSFNLRKPLSE
                     KIKESVRLIRIGDFDICACAGYHVSNTSKIGLIKIIDKEKIKGNLTRIYYVAGKRAFK
                     DYRDKNLILKGLSSLLTTSQNTLEKRVSDMIKELKDEKTLIKRISEEYAALFSVSLAE
                     KPLKCGKYNVLVYSKNDAVSGFLGKYIDVGKYVLVIGEEEDYSIATSLFDCKEFFSAL
                     QKKYGLRGGGNSSKVSFKGTITVEDILKEISII"
     gene            complement(8321..8926)
                     /locus_tag="PLU28_04225"
     CDS             complement(8321..8926)
                     /locus_tag="PLU28_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34306.1"
                     /translation="MFSLNLFLGHFIGEHAFSNVYSKSFIDKKGYYKHLIWIVLLFLA
                     LTFDSLLSSGAVFIVAALLMHVGVDLLRMKKKSSPLLELASLGAFLLLSIASTSFMKE
                     SYISWEFQLYLCGMMISTSFGSYLFRTFGLIDREKKDTTGATERLVIFIFMLSHQYLW
                     VFLSALIALGYKLMIEKRKDKELLLSPVYGILTSLIWMFIM"
     gene            complement(8938..9684)
                     /locus_tag="PLU28_04230"
     CDS             complement(8938..9684)
                     /locus_tag="PLU28_04230"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOG34307.1"
                     /translation="MSSIYSVYINLENSELKEKIDSLLVKNEIEIETEDIFNSDFAIV
                     REPLKFSFPHLLITKSISNIDYKNIIKNELIDFILEDYPQELVELKIQNATYFIMARG
                     SRGAFKQRIIQECEKLKRAGGTFSLALISVMNFYKLENSFSTVAIDEVSKELLTMMRL
                     SVRKSDEAMKISRREYGVILPFTELRNAEIACERVKRRTNKIGNYPDIDLAFGITEVS
                     NPNEDIIKILERLKRALYISEANNGEISFQ"
     gene            complement(9780..11072)
                     /locus_tag="PLU28_04235"
     CDS             complement(9780..11072)
                     /locus_tag="PLU28_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HOG34308.1"
                     /translation="MSKRLNPSIFKVPIDKIRTGYYADKYFTRFVEVLKKDGLKRNAL
                     YQFFPRKDSVIVGIDEALSILKFCTGYYKDEPKAMELFEKIQMLEKKLQEAAHRMDKA
                     ELLAITSQKWDYRMLLNELWVNKSSEIDVHALFDGEEARDMEPVLTIQGNPAYFGHLE
                     TVLLGVLARASSTATSVKKVVKASRGKPILFFSARFDHYWVQATDGYAALKAGAFGVS
                     TDANADYWGAESAGTIPHALIACYNGSTSEAAIAFDKYIDGSVNRMVLVDWDNDVIGT
                     TFKVVEDFYRHIKSEKFNWTREEADSIIGPGKNKIWGVRFDTSGTLRDLSVIPKDRSS
                     LGVNPELVWTARKEFDKRNLNNLKIVVTGGFDEEKINLFETLNVPVDVYGVGAKLLRE
                     KVDFTADIVEVDSQPCAKVGRGKGDLSRLQEVSSSYWL"
     gene            complement(11124..>11423)
                     /locus_tag="PLU28_04240"
     CDS             complement(11124..>11423)
                     /locus_tag="PLU28_04240"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOG34309.1"
                     /translation="FQKLSLFSGGEKALIAIAFLFSLMNLNPSPFYILDEVDAPLDDI
                     NAAKLSELIRENAAKSQFLVITHNKILMESAELFHGITMKKGITRVIPVDFKEFA"
BASE COUNT         3678 a         2071 c         2033 g         3641 t
ORIGIN      
        1 acgttcaaaa aaagcaatag agcaatcaga gatgattatt tttgttatcg atggtgaaac
       61 aggctttaca aatgaagata aagagcttta tgattttatt gtggcgcagg gtaaagataa
      121 tgtgattctt gcaattaata aaaaagatag tgtttctttt ataaaatgtg aagttccatc
      181 tttcataacc tggaaaacag ttgaaatttc tgcattgaaa aacgatattt ccgaacttga
      241 aaacgttatg tacagtatgc tgtgcgataa agtagaaata aatgagcctg ttcttacaaa
      301 ccttcgacag aagcaggcac ttatgaattc atacgaagct atgctggccg caaatagctc
      361 atacatgcag ggatacacat ccgatgtggt tatgatagat ataagaaaag ccattcaggc
      421 aattaacgag cttatagggg cagactatac acaggatctt cttgaaagta tattcagtaa
      481 tttttgtgtt ggaaaataaa tgaaaggagt ggatgatttg aaaaaacgaa ttatttctat
      541 attgtttgga ttgattccgt ttattcagtt tttcatagga ggatggattt tttttggtgt
      601 atctctgatt cttatgataa tatcgtactt tattataaga aaaaactctt ctcttaactt
      661 tgtagtatat gcacttgttc ttattgcaag tcaaattgtt gtttttatga acggcgcatc
      721 aaacattcct ttgtttgtct atgcggcaat agcatcagct ttatttctgg tagctgcttc
      781 tgatgaaaag gttatcgata atcttaggaa ggagcttaat caaactggtg taaagacaaa
      841 agaaggagtg tttgaacttt gttttttcgg tatgggagag ataaatgatc ttgaatctct
      901 ttcgggatta aaaacaacag ttgttttcat aggcgacgga agagcggcat ttaccgttcc
      961 aatgcccaaa agcactataa gcagaacaat aatgcttgat gatataattg attatggaat
     1021 gttccggctt gccaaaaagc aaactcccct atactatacc aagataagcg aaatgtttct
     1081 gccagccaaa agactgagga cacttcataa gccagaagtc gaaacatacg gactgtatat
     1141 agatactgcc gacgaacgat tgtcatttta cgaagaacca aaagtacttc aaaagcttga
     1201 aaatgctata aaaaggttaa aagaaaaaaa ataatggggg gatacatacg aaaagtctaa
     1261 gattaagcat attcgaacta aaaaaatttt ttagaaactc aggagtaatt tcgctgataa
     1321 taatccttcc tgttataata gcatatctcg gctcggtatt ttatcctgaa aacatacttg
     1381 caaactataa gatagctatt tataatgaag ataaaacgtg gctcgggcag tttggtttca
     1441 tatttctgaa acagtttttc aaatgggaaa atgccattga aattaaaaat gaagaggcat
     1501 tgagtactac tataaaaagt ggagactacg attcgataat cattattccc aaaggtttta
     1561 tgagtgatct taaggagtat aagcctacca agctcaatct tattcctaat cctcagaaaa
     1621 ttcaggatag tcttgcggtt tatacggttt ttaaagcact tttcagagag cttgcaggaa
     1681 taccagatct tgaaagcggt tccactctac agttccttat gtcgggagga attggagttg
     1741 atacccagcg taaagcgcca gatattgaga tacttgtccc taacatgaaa accgggagca
     1801 tcagatctga aattaaagcc gatataggat ttgaagatat atttgctcca agtgtggctg
     1861 taataatgat aatgattttc tgtatgatag gagtaggaaa ttcaattgcc cataccaaag
     1921 aaagcggttt gtttgatatc tacagatcta acggattaaa agtatatcag tttattaact
     1981 ttaaatttat cgcttacttt gtggttggac tagtggcaag tcttttttca tggtatatat
     2041 acaggctttt tggtgttcat tccatggcgg gagagtttga aataatattt cttattattg
     2101 tcacttcatt cagttttacg gctttcggta tacttttctc ttcgtttaca aaaacatcaa
     2161 aatcctccgg actgctctta acagctttaa caggctgtat gatgcttttt ggagatatac
     2221 ttattcctat tccacaggga agcattataa aaaatatagt aaatttcatg cccataaagt
     2281 attctgttga tgcgtggagg aaggtctcgg ttcttgggta ctccttgcct gaagtttcat
     2341 atgagatcct tattctgatt atctttggag ttgtttcata tctgctaagc aatattataa
     2401 tgacaagagt tgagaaaaaa taatgggtta ttattcatta aaaaagcaga gcagcgtcaa
     2461 attgaatata gaacgttcaa tttttatcgc atctgcagta aaagtatctt catgtcagca
     2521 ggcaaaagac ttcataaaag aaatatcaag gcattataat gatgcgacgc ataactgctg
     2581 ggcatataag ataattgaaa ataacagttt ccagtctgat ttttcagatg acagggaacc
     2641 ttccggaaca gctggaaggc caattttagg agcaattgaa aaaaacggta tcataaatgt
     2701 agcagttgtt gtgacaaggt actttggagg agtgaagctt ggtgtaaggg gacttatcca
     2761 ggcatatgcc caatcggcac agtcggctat agatctggcg ggagtggtac ttttcaaaaa
     2821 attctttctc tactcgttta aaacagatta ttccagcttt gcacagcttg aaaaaattct
     2881 caaaagggaa aaaggctggg catttaagca aaaaaaattt ttagatagca tacatgcaga
     2941 tattcttata aaagaagatt cgcttgaaaa aattttatct gttcttaaca ctaaggtatt
     3001 cgatcttgaa tatctagggg aagaagaagc ggaggaataa tttatggtga gcctatacag
     3061 aaagtacaga tccaaaacgt ttgatgagct tatcggacag gatcatgtca gaagatactt
     3121 tgcaaacgct cttgacagaa aagaaatttc acatgcttat atctttaccg gtcctagggg
     3181 aaccgggaaa accagtacag caaggcttct tgcaaaagtc cttaactgct gcgatcctga
     3241 tggatataat ccctgtaaca actgtgataa ctgtagagca atcgatgaag gttcgtttat
     3301 ggatgtaatt gaactggatg cagcttcaaa cagggggatt gatgagataa gagaaattag
     3361 ggattctaca aattaccgtc ctgttcacgg aaaatataag gtatatataa tagatgaatt
     3421 tcatatgctt actaaagaag cctttaacgc actgttgaaa acccttgaag aacctccgga
     3481 gcatgtaatt ttcatattag ccacaaccaa tctggaaaaa gttcctcaga caataatctc
     3541 cagggcgcag cttataaatt tcaaaaatat tcgtgaaaaa gatattgtta tgcttctaaa
     3601 aagagtaagt gctcaagaag gagttacaat tgatgaaaaa tccttgagta ttatctctaa
     3661 aagagcaaag ggaggagcac gagatgccct ttcgcttttt gaacaggtaa tgaaattttg
     3721 ttcaaaggaa gtcacttcat ttgaaacatt gagtatttta ggactttttg atgataacat
     3781 ggttgaagag tttataaact ttttgatctc tggagatgtt gataaccttt tgagactctc
     3841 atctgatctt tttgaaagcg gaaaagaacc tgaagcactt atagaacaga gtatcgaaaa
     3901 gctttttata aagataaaga acgataaaaa ttatgctctc gtaaaacttg tacgtgaatt
     3961 gaatgaagta agtaaagaac ttaagtacag tgagaataaa aaagctgtct ttgaaatata
     4021 tgttacatcg cttgcatacg ggtataaaaa attagatatc aaatatgaaa ctaatctgat
     4081 aaacgagtcg attaattctc ttgagcaaag cttttttact gaagattcag aacaaacttg
     4141 cggaggtgac tatcaactta tgcttcaaaa gcttgctaat gaaagttcaa aaatgaatct
     4201 gggacttttc tttgcgctga agaacaacac atacgaaatt tctgaaggta agcttacgat
     4261 attttttccc tctgagtctt caatggagta tcagattaca agaaagtatc tgagtgaatt
     4321 aaagataaat gttctgggta cttttaaagg cgtcagcgag gtaaaacttg tttttgacgg
     4381 tgatgtaaaa aaagattttc catctaatca ggatttatcc tcaaagctat tttcataaaa
     4441 ataacggaga gagagcctct ccgttacgat atttttatta ttttgtccat ttttatcagt
     4501 tggattactt ctctgacctt ggggctttta acgttttgga tctctacgtc accgcccgtt
     4561 tttttgtagc ttgtgaaaaa ataaaaaatc tttcctattc cgctgctgtc aacataatta
     4621 agacctatca tatcgaatat gactttttta tagtttttag tcttaatatc gttcaactct
     4681 gcttccaatg ccttggagtt ggaaatatca agtgagcctt caagataaac ccttaccgta
     4741 tcgcctttta tatctttcct catcttatca ccccttcctg atttattagg tccgtcatga
     4801 tctttttaag ctctgaattg cttttacctg aatactcgga aagaatctgg tttacgaaag
     4861 aaaggtcctc ttcaaaaatt ctgtagttag gatcagcatc tgttgatttt ataagtatct
     4921 ccagaactcc gtcatcgttc tcaagcatct ttacttcaaa ttccttgttc ttggccaaaa
     4981 aagcaactat gaacaaaagt ttttcttcaa aaatatgttt atagggaagt ttttcttcag
     5041 gctgtgaact gaaaaaatac ctgtaatttt ttttgtagta aaagtaatca ataagccaca
     5101 taagcttaag tatcttgata aagtcattct ctgaggaaga agctattata tgatgtatta
     5161 tataaaaaat tttctgcata tcataacttt tatgtatctg ctgaatctct gatttttgtt
     5221 ttttgttgtc catatcagat aaaagatttt ttactgctgc agatactttt tcgtaatcct
     5281 caggagaaat attttcttta ttctgctcaa caaagttcat gaaagcttta ggtgatcttg
     5341 cttgctttat ggtatcagag ttcactttgg agggaatcat accagtttca taacgccgta
     5401 tggtagcttc tcctattccc agtatctttg aaaaaagtga ttgtgaaata tcgtaaagct
     5461 ttcgtacctc tttaatctcc aaaggagata caagatcatg tttttttctg tagagctgca
     5521 agcctttttc catgttttct gaatgaatgc tttcaacata aaggttggat ttgcatactg
     5581 ggcagaaggt gcgtacaccg ttaaaaagaa taatctcccc ttttatggtc atcggaatag
     5641 tttcctcttt ttttattgct tcaacatact tattgcatct gtcacaaaaa atattggaat
     5701 ccataaccaa tcttaccccc atttaagaat gtttccgtgt tcggagaagt ctgcatgaac
     5761 ttccgaacta ctgtcaagaa aaaataaccg tctcaaaaat attttatcac atttgagatt
     5821 aaaatataca tttacgctat atttctgtct tttttccaga ggtttagata ataaaccact
     5881 ggaagaacca gaagataaaa agcaaaaggt ataaacccgc ttgttactcc taaagttgtt
     5941 gctggaacaa gacccgctat attccacgga ataagcggag atagaacaac agcagtattt
     6001 tcaatatcaa cggcaagttc gtaactgtca aaatttttct ttttataggt tttttttacg
     6061 atctgttctg tcaagattat agccataacc tgagtacatc caaacacagc agagactata
     6121 ctcacgatta tagttgaaaa gagagttccg cttctcgttt tgactctttc catgtatttg
     6181 tctataaaag agaaagcatc ggttccctcg aacaatcctg ttattgttga tgaaaaaaat
     6241 actataagag ccactttaag cattgaaagt atgccaccgc cagaaataat tttaaaaaga
     6301 ggattatcct tttcaaggaa gaagcctccg acagaaaact caagtacctc aaaaaaagag
     6361 tatccttgaa agaaaatact tattaaaaat cctgcgacta tacttacaag cattgaaagt
     6421 tttacattta ctctgaagaa agaaagaata ataattataa acgccgggag caatacccat
     6481 agatcggttc tgaaggtctt agctatatga aaaacaatgg tgctgtctga tgtattcaaa
     6541 ggattgaaaa ctgaaaagat catatagatg cctagggtga ttaaagttgc aagccaggct
     6601 gttttaaaca tgtttttaat gtttctgaat aaatctgttc ctgtcagagt tgcaacaagg
     6661 ttggcacttg aagacattgg tgagcacctg tctccgtaaa gagctccgga aattatagct
     6721 cctgcagcaa gttcaggact tattccgccg ctcctagcca ttattatcag agcaatgcct
     6781 atagtgctgg cggttccaaa ggatgttcct ataagcattg aaacaaaggc actcagaaca
     6841 aagcatgaaa aaatgaatat ataaggattc aaaagccgca ttccatagaa aactattgcc
     6901 ggaacagttc cgcttgacat ccaaactgca gttatggcac cgataagcac aaaaatttgg
     6961 agaaccacaa aggccttctt tcctccttta aaagcctgag aaagcaggta ctttaactta
     7021 aaaccccttc gaagaaaaat gccaataaag attaagtcta taagaatgag tgggtagaca
     7081 aggtatatgt tttttacaac actcagtata agaagaaaga cagcagctgc tattcctatg
     7141 aaaagatcca tcaaagcctc cgcctaaata attgagatct cctttaagat atcttcaacg
     7201 gttattgtac ctttgaaact tacttttgaa ctgtttcctc ctccacgaag tccgtacttt
     7261 ttctgtaaag cagagaaaaa ctctttacaa tcaaaaagag atgtagctat gctataatct
     7321 tcttcctctc caataactaa aacatacttt cctacatcta tatattttcc gagaaatcca
     7381 ctaactgcat catttttaga atataccaac acattgtact tcccgcattt taaaggcttt
     7441 tctgccagag agacggaaaa aagagcagcg tattcttcag agatcctttt tataagagtt
     7501 ttttcatctt taagttcttt tatcatatct gatactcttt tttcaagtgt attctgtgat
     7561 gtggttagaa gtgatgaaag acctttcaga ataagatttt tatctctgta gtccttaaaa
     7621 gcccttttgc ctgctacata gtatattctt gtaagatttc cctttatctt ttctttatca
     7681 atgatcttta taaggcctat cttcgaagta ttactgacgt gatagcctgc acaggcacat
     7741 atatcaaaat ctcctattcg gataagtctt acactttctt ttatcttttc acttaaaggt
     7801 tttctcaggt taaagctttt agaaccttca atatctgtaa gaatttcctc cacttgaaca
     7861 gcatcactaa cgattttgtt ggaaagctct tccatctcat ctgtaagctc ctgtgtaatg
     7921 aaaggaacat ccagatccac tgtggaatag ttttcatcca tacgaaagcc caccgtctgt
     7981 atattatgca cattttcaaa ggcagccgaa ataatatgct gggctgtatg ctgctgacat
     8041 atatcatttt ttctttcaga agatattgag tatttatgtt caccaggttc tacaaatcgt
     8101 tcggtctgga catacttttc ataacaggct aacacaggta tattctcgat cattcctcta
     8161 tctccgagct gtccgccttt accgtctgga tagaatgggg aattttcaat atatgaaaga
     8221 aaaattccgt ttttttcctc gctgtgtgtt acgatgatag tttccatgaa gcctcctatt
     8281 aaaaaaggct tgaaaaaatt caagccttaa cattataaga ttacattata aacatccata
     8341 taagcgatgt caaaattcca tatacaggag aaagcaaaag ctccttatct tttctttttt
     8401 ctatcataag tttgtagcca agagcaatta aagcgcttaa aaaaacccat aaatactggt
     8461 gggaaagcat aaaaataaat attaccagcc gttctgtagc tcctgttgtg tctttctttt
     8521 ctctgtcgat aagaccaaac gtcctgaata agtagcttcc aaaacttgta gaaatcatca
     8581 taccgcaaag gtaaagttga aactcccatg atatgtagga ttctttcata aatgacgtag
     8641 aagcaataga taataatagg aatgctccca gagaagcaag ctcaagcaaa ggtgaagact
     8701 tcttcttcat acgaagcaga tccacaccaa catgcataag caaagccgct actataaaaa
     8761 ctgcaccgga tgaaagcaaa gaatcaaaag taagggccaa gaataataac actatccata
     8821 tcaggtgttt gtagtaccct ttcttatcaa taaaagattt agaataaaca tttgaaaaag
     8881 cgtgttctcc tataaaatga ccaaggaaaa gatttaacga aaacatacta tcacctgcta
     8941 ttggaatgaa atttcgccgt tattggcttc tgaaatgtaa agtgctcttt tcagtctttc
     9001 gagtatctta ataatatctt cgtttggatt gcttacttca gttattccaa acgctaagtc
     9061 tatatccgga tagttcccta ttttattggt tcttctttta actcgttcac atgctatttc
     9121 agcatttcta agttctgtaa aagggaggat aactccatac tctcttctgg atattttcat
     9181 agcctcatcg gattttcgca ccgaaagtct catcatggtc agaagttctt ttgagacttc
     9241 atctatggcg acagtagaaa aggagttttc cagtttgtaa aagttcatta ccgatatgag
     9301 agcgagtgaa aaagtcccac cggctctttt cagtttttca cattcctgaa ttatcctctg
     9361 cttaaatgcg ccacggcttc cccgtgccat aataaagtaa gttgcattct gtattttgag
     9421 ttctaccaac tcctgagggt aatcctcaag gataaaatca atcaactcgt tcttgattat
     9481 atttttatag tctatattag atatggactt agttataaga aggtgtggaa aagaaaattt
     9541 cagaggttca cggactatgg caaagtcgga attaaaaata tcttcagttt caatctcaat
     9601 ttcattttta accaaaagag agtcaatctt ttctttaagc tcggagtttt ctaaattaat
     9661 ataaacagag tatatactgc tcatcaaatc ccctcccgta agcaatgtaa tcaaaccatt
     9721 actattatat catagatgtt gtaaggcgta taagatttgc taagattatt ttagagttat
     9781 taaagccagt aagaagacga tacttcctgc agtctggaaa gatcaccttt tcctcttcca
     9841 acttttgcac agggctgtga atctacttcc acgatatccg cagtaaaatc aactttttcc
     9901 cttaatagtt ttgccccaac accgtagaca tcaaccggaa cattaagcgt ttcaaagagg
     9961 tttatctttt cttcgtcaaa tccacctgtt acaactatct ttagattatt gagatttctt
    10021 ttgtcaaact cttttcgtgc agtccataca agttcaggat ttacgcccag cgatgacctg
    10081 tccttgggta ttaccgatag gtctcttaag gttccggagg tatcaaaccg tacaccccat
    10141 attttatttt ttcctgggcc gattattgag tcggcttctt ctcttgtcca attaaatttt
    10201 tctgatttta tgtgtctgta aaaatcctcc actactttaa aagtagtgcc tataacatca
    10261 ttatcccagt caaccagtac cattctgttc acagatccat caatatactt gtcaaaagca
    10321 atagcggctt cagatgtact gccgttatag caagctataa gtgcgtgcgg aatcgttcca
    10381 gctgattctg caccccagta gtccgcattt gcgtcagtag aaacgccgaa tgctcctgct
    10441 ttaagagcag catatccatc tgtggcctga acccagtagt gatcgaatct ggcactgaaa
    10501 aaaagtatag gttttcccct ggaggctttt acaacctttt taacacttgt agcggtgctt
    10561 gaagcccttg caagaactcc aagaagaacc gtttccagat gaccaaagta tgccggattt
    10621 ccctgtatag tgagaacagg ctccatatct ctggcctctt caccatcaaa aagagcatga
    10681 acatcaatct cagaagattt atttacccaa agttcattta gaagcattct ataatcccat
    10741 ttttgtgatg ttatagccag tagttccgcc ttatccatcc tgtgagcagc ttcctgaagt
    10801 tttttttcaa gcatctgtat cttttcaaaa agctccatgg ctttaggttc atccttatag
    10861 tatccggtac agaacttaag aatggaaaga gcttcatcta taccgacgat gacagaatcc
    10921 ttgcgcggga aaaactgata taacgcattc cttttcagtc cgtctttctt aagtacctct
    10981 acaaaacgtg tgaagtactt atcagcataa tatcccgttc tgattttgtc tatcgggact
    11041 ttgaatatgc ttggattaag ccttttactc ataatgcacc tcttctatat gataatagta
    11101 atagaaatac ataatccatg tacttatgca aactctttaa aatcaaccgg tattacccgc
    11161 gtaattcctt ttttcatggt aataccatga aataactctg cgctttccat aagaatttta
    11221 ttgtgggtta ttacaagaaa ctgtgatttg gcagcatttt cacgaataag ctcagagagc
    11281 tttgctgcat ttatatcatc gagcggcgca tccacttcat caagaatata aaaaggactg
    11341 ggattgagat tcatgagaga aaataaaaat gcaattgcaa taagcgcttt ttctccaccc
    11401 gaaaaaagtg aaagtttttg aaa
//
LOCUS       DAOSHR010000052        11379 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_4811_length_11379_cov_6.227393, whole genome shotgun sequence.
ACCESSION   DAOSHR010000052 DAOSHR010000000
VERSION     DAOSHR010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 11379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11379
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4811_length_11379_cov_6.227393"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1735)
                     /locus_tag="PLU28_04245"
     CDS             complement(<1..1735)
                     /locus_tag="PLU28_04245"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HOG34310.1"
                     /translation="MENETEVFTLKRNLSEFIKDGLDQEQLEAVTKSEGNTLIIAGPG
                     SGKTRVITYKIAYLLDNHVRAENILLVTFTRAAAKQMIERVKKVTEKDTSQLVAGTFH
                     HVCNLILRKYANILGYANSYSILDSEDADDLLKMARNEYRSEYSLDKELPKESVVSKI
                     ISYACNTVKSVREALGDIAPYLMEFEGDIEKIWMKYAQMKKDMNSMDYDDILVNTGHL
                     LSTNPDILKKVSSQFRYVLVDEFQDTSKIQLEIVRALSSVHKNLIAVGDDAQSIYSFR
                     GARFENIQEFMEIKGTKLFKIQKNYRSVPEIVQLINTMLPEKAIPKKLESVRKTGVLP
                     FMIETFDDSEQSEVVCKIIADKIDEGLEYKDIAVLYRTHSLSMVLQQKMDSKRLPYRL
                     LSGRKFIETAHIKDMLSFVKVLQNPLDKISWSRALKLFPGLGAKTAIRIYEEIGSELL
                     KESDFETALKNSSIKKNKDALKIFSTLISMSEQNPSKVLEYIYNTFYQEYSRLSFPDY
                     RSRNSDVERFIDIAGRYDNISRFLEEMVLSENVQVIAPSQEDKENKITMSTIHGAKGL
                     EWKVVILISVNP"
     gene            complement(1743..2951)
                     /gene="rpoD"
                     /locus_tag="PLU28_04250"
                     /pseudo
     CDS             complement(1743..2951)
                     /gene="rpoD"
                     /locus_tag="PLU28_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208484.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
     gene            complement(3034..4758)
                     /gene="dnaG"
                     /locus_tag="PLU28_04255"
     CDS             complement(3034..4758)
                     /gene="dnaG"
                     /locus_tag="PLU28_04255"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOG34311.1"
                     /translation="MNNYDQLKETVQKIKDSLSIVDVISSYISVKKAGRNYSALCPFH
                     PEDTPSFYVFAETNTYHCFGCGAHGDAINFVKEYEKVSYVQAVKKLAAQSGIQIELEE
                     RPEIPELRLNREVAVAYSDTLMNLPDSSEIWKYLKNRSIDRQAAKEFELGFSTGKEVM
                     QVINKSDFKVELAIRNGLYNQNREFFNNRLIIPIRDNNGDLSGFSARSIDGSSPKYLN
                     TPETQYFKKGKILYLYHKTKNYIKQNDFAIIVEGYFDAISMYRAGFKNVAAVLGSVLT
                     KEHAFELMKSTNKIITMFDSDEAGRKATLSSIDNLYTKGFQIAVAKYDEKDPDELVRS
                     NQNKYIAELLKKSYKFHEYIPHAYVSRYDIQNDFGLEEYLREMSVWYKKFTESKRFEI
                     LDTFISTIAQITKKRDEIIEEIIKSKIQTLNFKADSIIQNQQKHQNLEKEIRYDFDRS
                     IIYLWLKYDDYKEKLNELDSGAIDEGPAKEFLRFVNEGKNITQILENGSVQLCEMISQ
                     IYLIDSFFEPGKIYDALKMTFERKTFQKHIDSLKNKLKGTVDEEERAKIALEIINCIS
                     KQKSLGGK"
     gene            complement(4835..5242)
                     /gene="rpsI"
                     /locus_tag="PLU28_04260"
     CDS             complement(4835..5242)
                     /gene="rpsI"
                     /locus_tag="PLU28_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOG34312.1"
                     /translation="MAELLDYYGTGRRKTSIARVHLRPGTGKFTINGKEYDDPVAYFN
                     NNKIWTRHAFEPLTVTQNENKFDVYIIVEGGGLNGQSGAVRLGVARALLELDESTRPV
                     LKVRGMLTRDSRAVERKKYGLRKARKRPQFSKR"
     gene            complement(5260..5706)
                     /gene="rplM"
                     /locus_tag="PLU28_04265"
     CDS             complement(5260..5706)
                     /gene="rplM"
                     /locus_tag="PLU28_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOG34313.1"
                     /translation="MASIMTQKTYMAKPAEVKRKWYLVDAQGKTLGRLASQVSKILQG
                     KNKPTYTPHIDTGDFVIIVNAEKICVSGRKMTQKVYYRHTGFPGGLKVSKITEVFDKK
                     PERVLEMAIKGMMPKSILSKHMIKKLKIYAGSSHPHEAQKPEKIEL"
     gene            complement(5706..6266)
                     /locus_tag="PLU28_04270"
     CDS             complement(5706..6266)
                     /locus_tag="PLU28_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34314.1"
                     /translation="MSSKRLAVSGIIGALAFLLTFIEFPIFPFAAFLRFDPSISLIVV
                     MVQAFGWIPALFSLLIKDLLFTLIHQGAGGPIGMLMNTTAGLVFVSILSLLKTKTAKY
                     IGYSLSSLAASLVMLIMNFLFLPLYTGMSFADAGSALGVGTSGLILTIIGFNIIKFLA
                     NSFIGDIAYSRIKKIFNNKDLKGGSK"
     gene            complement(6289..6558)
                     /gene="rpmA"
                     /locus_tag="PLU28_04275"
     CDS             complement(6289..6558)
                     /gene="rpmA"
                     /locus_tag="PLU28_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009382703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOG34315.1"
                     /translation="MKLNLQFFAGAAGDANRRDSNPKYLGVKAGEGKLVTSGSIIIRQ
                     RGTKIHPGKNVGLGRDFTIFATADGRVKFEEKNNRKYVSIYPQAQ"
     gene            complement(6558..6848)
                     /locus_tag="PLU28_04280"
     CDS             complement(6558..6848)
                     /locus_tag="PLU28_04280"
                     /inference="COORDINATES: protein motif:HMM:NF016233.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HOG34316.1"
                     /translation="MIRVSVEGARMTIKGHAGYGAYGKDIVCSAVSVLTQFVGEIIEK
                     EKMGSYKIMENTIQLDFDPDNVISLKLQSYLLEAFKNIETDYKKNMKVEVSS"
     gene            complement(6852..7166)
                     /gene="rplU"
                     /locus_tag="PLU28_04285"
     CDS             complement(6852..7166)
                     /gene="rplU"
                     /locus_tag="PLU28_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOG34317.1"
                     /translation="MYAILEFGGKQYKVEEGQTLYTEKVNGVEPGSSFVFDKVLFVKK
                     DDSVKVGSPYVQGAKINAEVIEHGKDEKILVVKFRRRKMYRRVNGHRQQFTALKITKI
                     EA"
     gene            complement(7281..7793)
                     /locus_tag="PLU28_04290"
     CDS             complement(7281..7793)
                     /locus_tag="PLU28_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34318.1"
                     /translation="MNVERITSFGVRIDGMYIVANGIVLGVIYGFETNRLFRIFSTGD
                     IIGLDKYYCNIGLIDYFGIGDFELDNLSDDEFKEKLKDKSFCKLFSESLKELFSELSS
                     FSKEDEQIILYYNMKKLYQMSGSKRLGLPKKFVEETGIDRVMAELQTQGKVLLDKDVY
                     IINRQSVTEN"
     gene            complement(7795..8616)
                     /locus_tag="PLU28_04295"
     CDS             complement(7795..8616)
                     /locus_tag="PLU28_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34319.1"
                     /translation="MKINKYKKGKVIIEKDSPVKKLFYIQQGKITDTKNIFSSGSFFG
                     LVSFFTELNVLNDYRILENAVLYEYDCMDEIPEDILTFFIKSLSDMYVGFSYMSLVGR
                     MPDPSFIISNYNDQKSSAAGGSKKDLLKELKGDGDLDDFIKALDFYINDNDEEVEIPQ
                     TFSDFKDEINKLLVEFNVSRFIAMMRGVIVKKSFPAENWYAFFSELFERSITLNDRAL
                     TLYIIYSSSFMLTDLEQINSLLRKHWEFLRFNGLNSWIDYVLRVHNNKLIVQVDD"
     gene            complement(8603..10747)
                     /locus_tag="PLU28_04300"
     CDS             complement(8603..10747)
                     /locus_tag="PLU28_04300"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HOG34320.1"
                     /translation="MKKYFRSFFIGFTVGMCFFAFLFIYTYNFYKNSNSIFFINSNYN
                     TYLFRNGELILPTKYKYVKLADMPVNLINTLLWSEDRDFYEHYGFNLKSFIRAVVINA
                     FTLDFSQGGSTITQQLAKMVYLSNEKTIKRKMLDIMLAFFIERSYSKDEILEAYMNNA
                     AYLGNDILGFPAAADRYFKKNINDLSFAEMAVLVGIINGPSIYDPYRHPDRARKQAQA
                     LLNSLGGSSLKNTIDLKKAAEDLEKMEFKKFTYEDRYVELIYQCISELKELNLKSGGY
                     VIKSTFDKDIFESVTMPATACGIAINNKTGEMLGFWGSQYSAFLSQQQIGSGVKPFYY
                     SMAIENGYTFDTILPDKPTDFGGWKPQNFDKKFRGEVTLKESLVNSINISSIYLAMHV
                     STSPTNSIQKIEDFLKKNIGIKGKYPGDLTLSLGTLETNPYNMVRAYTMFSNYGLIPK
                     IFSVFEVYDRRGNIIFRQNPSIERKVEGISPQTYASMVQLLRETVVSGSAKRANVQGV
                     ELVGKTGTAESAAWFTGDTGSTSLSVRVDGKDLLSSTAAVPIAQTIVKSFYYNTPNYK
                     IPLYTSVKGLNKLDFFADPVTFISKGMDVISYLANAKLSEGIERIGEKIEEVITDIEF
                     IYPDMALKIRQWYAVNSVDFLDDPYTFVLNGSDISVYLQKLEINEEKAIRLMEISQEL
                     SSSYPDVSMKINEYLKEKGYETALRGNSHEDQ"
     gene            10831..>11379
                     /locus_tag="PLU28_04305"
     CDS             10831..>11379
                     /locus_tag="PLU28_04305"
                     /inference="COORDINATES: protein motif:HMM:TIGR03936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HOG34321.1"
                     /translation="MKYLLRFKKYSCLSYISHNDIARTVEMFMRRSGCPMDYSQGFHP
                     MPLISYSPAIPLGFINRAVYLTIQTKDHFDFSTVSGSIQKGLDLLEHTETTPSFKLSA
                     EIKGYRFKCYLSENTFKKLISYENSTIEKGKNSYDFKKLFMDFTYCVNKKGFFMIQYI
                     QESDMLFNPFKLVKDGFEPEKDF"
BASE COUNT         3199 a         2428 c         1825 g         3927 t
ORIGIN      
        1 cgggattgac agatataagt atgacaacct tccattccag acccttggct ccatggatgg
       61 tgctcatggt tatcttgttt tctttatctt cctgtgaagg agcaatcacc tgaacatttt
      121 cactcaaaac catctcttct agaaatcttg aaatattgtc ataccttcca gcaatgtcta
      181 taaatctttc tacatcagag tttcgtgaac ggtaatccgg aaaggaaagc cttgaatact
      241 cttggtaaaa agtattatat atatactcca gaacctttga aggattttgt tccgacatag
      301 atataagtgt ggaaaatatt ttcagagcat ctttgttttt cttaatggat gaatttttca
      361 gtgccgtttc aaagtcgctt tctttaagaa gttcagaacc aatttcttca tatattctga
      421 ttgccgtttt ggctcccaga ccagggaaaa gtttaagcgc cctgctccat gaaattttgt
      481 caagaggatt ctgaagaacc tttacaaaag acagcatatc ttttatatgt gccgtttcta
      541 tgaactttct tccggaaaga agtctgtacg gaagtctttt ggaatccatc ttctgctgta
      601 aaaccataga aagggagtgt gtcctgtaaa gtacggctat atctttgtat tcaagacctt
      661 catcaatttt atctgcaatt atcttacata cgacttcact ttgttctgaa tcatcaaaag
      721 tctctatcat gaaaggaaga actccggtct ttctaacgga ttcaagtttt ttgggaatag
      781 ctttttcagg aagcattgtg ttgatcagtt gaactatttc cggaacgctc ctgtagtttt
      841 tctgaatttt gaagagcttg gttcccttga tttccataaa ctcctgtatg ttttcaaatc
      901 ttgcaccacg gaatgaataa atgctttggg catcgtctcc cactgcaatc agatttttat
      961 gaacgcttga caaggcacgc actatttcaa gctgaatctt gctggtatcc tgaaactcat
     1021 ctaccagaac atacctgaac tgagaggata ccttcttaag tatgtccgga ttagtcgaaa
     1081 gaagatgtcc agtattcacc agaatatcgt catagtccat tgaattcata tcttttttca
     1141 tttgggcata cttcatccat attttttcta tgtccccttc aaactccata agatatggag
     1201 caatatctcc aagggcctcg cgaactgatt tcactgtatt gcatgcgtaa cttattatct
     1261 tacttacaac agattctttg ggtagttctt tgtccaaaga gtattcactt ctgtactcgt
     1321 tccttgccat cttcagaaga tcatcagcat cttctgaatc aagaatggag taactgtttg
     1381 catatcccag aatattggca tacttcctca gtataagatt gcacacatga tgaaaagtac
     1441 ctgcaacaag ctgcgaggta tccttttcgg ttactttttt tactctttct atcatctgtt
     1501 ttgctgcagc acgcgtaaat gtcaccaaaa gaatattttc cgctctgaca tgattatcca
     1561 gaagatacgc tatcttatag gttatcactc tggtctttcc ggaacccggc ccggcaatta
     1621 taagtgtatt cccttcggat tttgtaactg cttcaagctg ttcctggtca agtccgtcct
     1681 ttataaactc tgacagattc cttttcagag taaatacttc agtctcattt tccatctttc
     1741 acctaaaaaa tctttttacc aagcatagag ctaagatcct tcagttgggc gctcctgctc
     1801 ggatgcctta gttttcgcag tgccttgacc tcaatctgtc ttattctctc tctggtgacg
     1861 ttgaagaact gccctacctc ctcaagagtc tttactttac cgtcaaaaag accgtatctc
     1921 atcttaagca cggttgcttc tttgggttga agagtgtcca gtatcttctc tatctcttct
     1981 cttattatca tcttaactgc tgcttcttca ggatgatcaa cggcatcatc tgcaagaaaa
     2041 tctcccagag tggagtcatc ttcatcagat ccggttattg gagagtcggc agaaatggtc
     2101 tccctcgctg caagaagtat ctcacttatc ttatcaacag gtttgtccat gagttttgaa
     2161 agatcctgta cggttggata ttcaccattt tcctgtagaa actcccttat cactttgttc
     2221 agcctgttga tggtttcaac catatgaacg ggtattctga ttgtccttgc ctgatcagct
     2281 atagctcttg ttatagcttg ccttatccac caagtagcgt atgtggaaaa tttaaaacct
     2341 tttttccagt caaatttatt tacggctttt ataagaccta tatttccttc ctgaattaaa
     2401 tccagaaaac tcaagcctct gccaacatat ctcttggcaa tgcttatgac aagcctcaaa
     2461 ttagttttta tcagttcatc tcttgatctt ttatctccgg cctgagccct ctgtgccaat
     2521 tgcctttccc ttcttggggt gagcaaccct attttgctta tttccttcaa atatattttt
     2581 atcggatcat taagcgatat attgtcatac atctgcattt cgccatcacc aagtatgtcg
     2641 agcacctcat actcctcggc ggccggttcg gcaaactcat cttcggaaag aagaaaatta
     2701 tcatcaccca taatatcctc tgtttctccg tccaagcttg aatctattat ctctatatcg
     2761 aacttctgca gttctgtata tattttttca agataatcgg cgtcaatcat ctccgagtct
     2821 tccggaatac acttatctat atcctcataa gtaagcttat tgctgttttt ctttgccaaa
     2881 tcccttaaat cttttataat tgcttctata gttttttgcg gtttctgatt cttgttcttg
     2941 agctttatct tgtcatcctc aaggatctca agatctccaa gctcctgtat agtatcagct
     3001 acctcaagat tcagatcgtt tgccataacc atcttattta cctccaagac ttttctgttt
     3061 tgaaatgcag tttattatct ctaaagctat tttagcccgt tcctcctcat caactgtacc
     3121 cttaagttta tttttaagac tatcaatatg tttctgaaag gttttccttt caaaagtcat
     3181 ttttaatgcg tcatatattt ttccgggttc aaaaaaacta tcgataagat agatctgaga
     3241 gatcatctca cataactgta cggaaccgtt ttcaagtatc tgtgtaatat ttttgccttc
     3301 atttacgaac cttaaaaact ctttggcggg tccttcatca atagccccac tgtcaagctc
     3361 attaagcttt tccttgtaat catcgtattt cagccacaga tagataatag acctgtcaaa
     3421 atcatatctt atttcttttt cgagattttg atgtttttgt tgattttgta ttattgaatc
     3481 tgctttaaaa ttcaaagtct gaatctttga cttgattatt tcctctatta tttcgtcgcg
     3541 cttttttgtt atctgggcaa tagtgctgat aaaagtgtca agtatctcaa accgtttgga
     3601 ttctgtaaac tttttgtacc acacggacat ttccctgaga tactcttcta gtccaaaatc
     3661 attttgtata tcgtatcttg aaacatatgc atgcggtata tattcatgga acttataaga
     3721 tttcttaaga agctcggcta tgtacttgtt ttgattactg cgtaccagtt catcgggatc
     3781 tttttcatca tacttagcaa cagcaatctg aaaacctttt gtataaagat tgtctataga
     3841 agatagtgta gctttgcggc cagcttcatc tgaatcgaac attgtaatga ttttgttcgt
     3901 ggatttcatt aactcaaagg catgttcctt ggtgagaacc gaaccaagaa ctgctgcaac
     3961 atttttaaat cctgccctgt acattgaaat tgcatcaaaa tacccttcta ctataattgc
     4021 aaagtcattc tgctttatat aattttttgt tttatgataa agataaagta tctttccttt
     4081 tttgaagtac tgtgtttccg gagtgttaag atattttggc gatgagccat ctatactgcg
     4141 ggcagaaaac cccgaaagat ctccattatt gtctcttatc ggtattatca gcctgttatt
     4201 gaaaaactcg cggttttgat tatacaatcc atttctgatt gcgagttcaa ctttaaagtc
     4261 acttttatta attacctgca taacttcttt accggttgaa aaacccagtt caaactcttt
     4321 tgccgcttgt ctgtcaatac tcctgttttt gaggtatttc catatttcag aactgtcagg
     4381 aaggttcata agtgtatcgc tataggcaac agctacctct ctgttaagtc ttagctctgg
     4441 tatttctggc ctttcctcta gttcaatttg tattccgctt tgtgctgcca gttttttcac
     4501 agcctgaaca taactaacct tttcatactc tttgacaaaa tttattgcat cgccgtgtgc
     4561 gccgcaacca aaacaatgat atgtgttggt ttctgcaaaa acatagaaag aaggtgtgtc
     4621 ttctggatgg aacggacaca atgccgaata attcctgcct gctttcttta ccgatatata
     4681 tgaacttatt acatcaacta tgcttaatga gtcctttatt ttctgaaccg tttccttaag
     4741 ttgatcgtaa ttgttcattt ctatacctct ttgaaaaaaa gaacggcgtt cgaatgccgt
     4801 tcttttaatt ttatttctga tgttccaaaa aatactacct cttggagaac tgtgggcgtt
     4861 tccttgcttt tcttaatccg tacttctttc tttcaactgc tcttgaatct ctagtaagca
     4921 ttcctcttac tttaagaaca ggtcttgtgc tttcgtcaag ttcaagaaga gctcttgcaa
     4981 ctccgagtct tactgctcct gactgtccgt taagtccgcc gccttcaact ataatgtata
     5041 cgtcaaactt attctcattc tgagttactg taagaggttc aaaagcatgt ctggtccata
     5101 ttttattgtt attaaaatac gcaacgggat catcatactc tttaccgttt atagtaaatt
     5161 tgccggttcc cggtctgaga tgaaccctgg caattgatgt ttttcttctg cctgtaccgt
     5221 aataatccaa caattcagcc attacaatct cctcctgttt tatagttcta tcttttctgg
     5281 tttctgtgct tcgtgcggat gactagaacc agcatatatc ttaagttttt taatcatatg
     5341 cttggaaaga atgctttttg gcatcattcc ctttatagcc atttcaagaa ctctctccgg
     5401 ctttttatca aaaacctctg tgatttttga aaccttaagt cctcccggaa agcctgtatg
     5461 tctatagtat accttctgag tcattttcct gccgcttaca catatttttt ctgcatttac
     5521 tattattaca aaatcacctg tatcaatatg cggagtatat gtaggcttat tttttccctg
     5581 taaaattttt gaaacctgag aagccaatct tcccagagtt ttaccctgag catccacgag
     5641 gtaccatttc cttttaacct ctgccggctt agccatgtaa gtcttctgtg tcattataga
     5701 agccattatt tgcttcctcc tttcaagtct ttattattaa aaatcttttt tattcttgaa
     5761 tatgctatgt ctccgataaa agagttcgcc aggaacttaa ttatgttaaa acctattatg
     5821 gtaagaatca gcccggaagt tccaacacca agggcagatc ctgcatctgc aaagctcatg
     5881 cctgtatata acggaagaaa taaaaaattc attataagca ttactaaact tgcagccaaa
     5941 ctggataatg aatacccaat atacttagct gttttggtct tcagcaatga caggatagac
     6001 acaaaaacca gcccggctgt agtattcata agcattccta tcgggccgcc cgctccctgg
     6061 tgaatcaagg taaaaaggag atctttgatc aaaagggaaa acagagctgg aatccatcca
     6121 aaagcctgaa ccataacaac tataagcgat atgctcgggt cgaacctaag aaaagcagca
     6181 aacggaaata ttggaaactc tatgaaagtc aacaaaaaag ccagtgctcc tattatacct
     6241 gatacagcca acctttttga actcataagt caccttccat aggctgactt actgtgcctg
     6301 aggatatatg cttacgtact ttctattgtt cttctcttca aacttcaccc tgccatcagc
     6361 agtggcaaat attgtaaaat ctctgcctaa accgacgttc ttgccggggt gaatcttggt
     6421 gcctctctgc cttattatta tgcttcctga tgttaccagc tttccttctc ctgcttttac
     6481 accaagatac ttagggttgc tgtctcttct attggcatcg cctgccgcac cagcaaaaaa
     6541 ttgtaggttg agtttcatta gcttgacacc tccactttca tattcttttt atagtctgtc
     6601 tctatgttct taaaagcttc taacaggtaa ctctgcagtt tcaacgaaat aacgttgtcc
     6661 gggtcgaaat ctaactgtat cgtgttttcc attattttat atgatcccat tttttctttt
     6721 tctataattt cacccacaaa ctgcgtcaga acgcttacag cactgcatac tatatcctta
     6781 ccatacgcac cgtagccggc atgacctttt atagtcattc ttgctccttc tacgcttacg
     6841 cgtatcataa cctaagcttc tatcttggtt atctttaagg ctgtaaactg ctgtctgtgg
     6901 ccgtttaccc ttctgtacat ctttcttctt ctgaacttta caaccagaat tttttcgtct
     6961 tttccatgtt caatgacttc ggcgtttatt ttggctccct gaacatatgg agatcccact
     7021 ttcaccgaat catctttctt tacaaacaaa accttgtcaa aaacaaagga agaaccgggt
     7081 tctactccgt ttaccttttc tgtgtacaga gtttgacctt cttccacttt gtactgcttt
     7141 cctccaaatt ccaatatggc gtacataatg tcacctcctg catttatgaa ataatccgcc
     7201 tgggatacaa ggtatcttag aaaaagaatt tataacctta tcccgagata atattttatc
     7261 atatataaaa actcactcta ttaattttct gtaacagatt gcctgtttat aatataaaca
     7321 tctttatcaa gaagcacttt tccttgagtc tgaagctcag ccataacccg gtcaattcct
     7381 gtttcttcaa caaatttttt tggcagaccc agacgtttgc taccgctcat ttggtacagt
     7441 ttcttcatat tgtagtagag gataatctgc tcgtcttcct ttgaaaatga tgagagctct
     7501 gaaaaaagtt ctttaagcga ttccgaaaat agcttgcaaa aacttttatc tttaagtttt
     7561 tccttaaact catcgtcaga aagattatcc agttcaaaat cccctatccc gaagtaatct
     7621 ataagcccta tattacaata gtatttatca agcccaatta tatcaccggt tgaaaagatc
     7681 ctgaacaacc tgttagtttc aaaaccatat ataactccaa gcacaatacc gtttgctact
     7741 atatacattc cgtcaatcct tacaccaaag ctggttattc tttctacatt catactaatc
     7801 atcaacctgt actatcaatt tattgttatg tactctcaga acataatcta tccatgagtt
     7861 taaaccatta aaccttaaaa actcccagtg ctttctcaaa agcgagttta tctgctccag
     7921 atccgtcagc ataaaagatg agctgtatat tatatataac gtaagcgccc tgtcattaag
     7981 ggttattgac ctctcaaaaa gttcagaaaa aaaagcatac cagttctcgg caggaaagct
     8041 cttttttact atgacacctc tcatcatggc tataaaccta gaaacgttaa actccacaag
     8101 aagcttgttt atttcgtcct taaaatccga aaaggtctgc ggtatttcaa cctcttcatc
     8161 gttgtcgtta atgtaaaaat ccagagcttt tataaaatca tcaagatctc cgtcaccttt
     8221 aagctctttc aaaagatctt ttttagaacc accggcagcg gaacttttct gatcattgta
     8281 attacttatt ataaaacttg gatccggcat tctgcctaca agactcatat aagaaaagcc
     8341 tacgtacata tccgaaagag actttataaa aaaagtcagg atatcttctg gaatctcatc
     8401 catacaatca tattcatata gaaccgcatt ttcaagtatt ctgtaatcat taagcacatt
     8461 aagctcagta aagaatgaaa caagtccgaa aaaagaacca ctgctgaata tgtttttggt
     8521 atcggtaatc tttccctgct gtatataaaa aagttttttt acaggactgt ctttttctat
     8581 tattaccttt cctttcttat acttattgat cttcatggct attccccctt aaagctgttt
     8641 catatccttt ttctttaagg tattcattga tcttcatgct cacatccgga taagaggaag
     8701 aaagttcttg tgaaatctcc ataagtctta tggctttttc ttcatttatc tcaagctttt
     8761 gcagataaac tgaaatatct gagccgttta ggacaaacgt atacggatcg tccaaaaagt
     8821 caaccgagtt cacggcatac cattgtctta tcttcaacgc catgtcagga tatatgaact
     8881 caatatctgt tatcacttct tctatttttt cgcctatcct ctcaattcct tcagaaagct
     8941 ttgcattcgc aagataactt attacgtcca tgccttttga gatgaatgta acaggatcag
     9001 caaagaaatc aagcttattt aaacctttta cagaggtata aagcggtatc ttatagtttg
     9061 gcgtattgta ataaaaagat ttcactatcg tctgcgcaat aggaacagct gcagttgaag
     9121 ataaaagatc ctttccgtca acccttactg ataggcttgt gcttccggta tcacctgtaa
     9181 accacgccgc actttcggca gttccggttt ttccaaccag ttcaaccccc tgtacatttg
     9241 cacgtttagc gctcccagaa acaacagttt cccttaataa ctgaaccatg gaagcatagg
     9301 tctgcggcga aattccctct acttttcttt caatagaagg attttgcctg aaaataatgt
     9361 ttccgcgtcg gtcataaact tcaaaaactg agaatatctt aggtataagt ccataatttg
     9421 agaacatcgt ataagctctt accatattgt atggattagt ctctagtgtg cccaaagaca
     9481 gagtcaggtc tcctggatat ttgcccttta tacctatgtt cttcttaaga aagtcttcta
     9541 ttttctgtat ggagttggtt ggcgatgttg aaacatgcat agcaagataa atagaagaaa
     9601 tgtttatcga atttaccaag gattctttaa gtgttacttc acctctgaac ttcttatcaa
     9661 agttttgagg tttccatcct ccaaagtccg taggcttatc gggcagtata gtatcaaagg
     9721 tatagccgtt ttctattgcc atggagtaat agaaaggctt tactccggat cctatctgtt
     9781 gctgggaaag aaatgcagag tactggctgc cccaaaatcc aagcatctca cccgttttat
     9841 tatttatagc aataccgcat gcagttgcgg gcatagtaac ggattcgaat atatctttat
     9901 caaacgtgct ttttatgaca tatccgcctg atttcagatt aagctccttc agttcgctta
     9961 tgcattgata gataagttca acataacggt cttcatatgt aaatttttta aactccatct
    10021 tttccagatc ttctgctgcc tttttaaggt caatagtatt cttaaggctt gatcctccca
    10081 gagagttgag tagtgcctgc gcctgtttcc tggctctgtc gggatgacgg tatgggtcat
    10141 atatagaagg tccgtttatt ataccaacca aaacagccat ctctgcaaaa ctcagatcat
    10201 ttatattttt tttgaagtac ctatcagctg cagccggaaa tccaagtata tcgtttccaa
    10261 gataggctgc attattcata tacgcttcaa gtatttcatc cttagagtag gatctttcaa
    10321 taaaaaaagc aagcattata tcaagcatct ttctttttat ggttttttcg tttgaaagat
    10381 aaaccatctt tgccagctgt tgggttatgg tgcttccgcc ctgtgaaaaa tccagtgtaa
    10441 aagcatttat aactactgct cttataaaag atttgaggtt aaacccgtaa tgttcataaa
    10501 aatccctatc ttcagaccat aaaagtgtat ttataaggtt cacaggcata tccgcaagct
    10561 ttacatactt gtacttggta ggtaagatta gttcaccgtt cctgaacaga taagtgttat
    10621 aattagagtt aataaaaaat atggagtttg aatttttata aaaattgtat gtgtaaataa
    10681 aaagaaaagc aaaaaaacac atacctaccg taaatcctat aaaaaaactt ctgaaatatt
    10741 ttttcactga atttcctccc aaagttcttt ttactaagta tttctcgtat caaaattatt
    10801 atatcatatc tcattccgag gtgcatacaa atgaaatatc tattaagatt caagaaatat
    10861 tcatgtcttt catacatatc ccacaacgat atagccagaa cagtagaaat gttcatgaga
    10921 agatccggtt gtcctatgga ttattcacag ggctttcatc caatgccgct gatctcatac
    10981 tcacctgcta ttcctctggg atttataaac agggctgtgt atcttaccat acagactaag
    11041 gatcattttg atttttcaac ggtatcgggc agtatacaaa aaggattaga tcttttggaa
    11101 cacacggaga ctactccttc tttcaaactt tcggcagaga ttaaaggata ccgttttaaa
    11161 tgctatcttt ccgaaaatac tttcaaaaag ttaatttcat acgaaaattc aactatagaa
    11221 aaaggaaaaa acagttatga ttttaaaaaa ctgtttatgg atttcacata ttgcgtgaat
    11281 aaaaaaggct tttttatgat acaatatata caggaaagcg atatgctttt caatcctttt
    11341 aagctggtta aggatggatt tgaaccggaa aaggatttt
//
LOCUS       DAOSHR010000053        11280 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_4863_length_11280_cov_5.835902, whole genome shotgun sequence.
ACCESSION   DAOSHR010000053 DAOSHR010000000
VERSION     DAOSHR010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 11280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11280
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4863_length_11280_cov_5.835902"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2374
                     /locus_tag="PLU28_04310"
     CDS             <1..2374
                     /locus_tag="PLU28_04310"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOG34322.1"
                     /translation="KLDVKKGDSIELLDSSNPLYILLPPTFTVVDVLSEKGLFNYREE
                     GSFISGAIFVSLPTTRRFAKLDDGYFSHIIISNTGDSLKGERLSDRIRQVAEKFSTSL
                     PGKNTTQRYLKSEQLRAVTGNGISLVFIILSSFAIIAGAVLITNMYDMLIQERKSELG
                     IMRAIGFTRSWIRKVMFYEGFFYSLFSLPVGIVFGTLVSLFTFSKVNVLGEQFSTFEF
                     TSGSIPVMSSYSISLQSMITGGILGMMIPLLIIVLGSSKIGRLNISDSIRNISDFREK
                     QKKLEFFLWFLMIISIPFIFFKNPSIRYAAVSIFIFMLPYTVLKKISKYSLNLSVLAV
                     IAFTLAFRSEGIILAGLKALIVLIAVILLFVYNLKPLERIITFLIKLITRKSTRATPI
                     MRIAFSYPSFNKKRTSLTTVLYGMVIFVIVIITIIPYSQTQRLAKSRNELLGGNDGFV
                     ISLPFLPSRNRPDVSKISETQGISNVSQFGFVMTQDSEGESYPLILLSDDLIDSSHID
                     SKFSSISSEFKALSEREVWNLLKTHSDYTIVKSLPGSKLKVDQSISLKVNEFDFNSAS
                     QLKPSDTAQTINLRVIAIVSQANNSPIVGPVVSERSVMGMIDQSKIITGFLFKVSDTS
                     RETYKNISNSFKINDYLFIFVDDIIDFVSKITAGAVDILSSFLYFGLVVGILGISVTT
                     IKSIEERRNAIGMLKSIGFTSRMVFWSFFLETSFIIVMGILIGVLAGSIASYEIYTQI
                     LKDSAQYFMIPYSKLLGMSAVFYLISIIFTYFPCRQASKISPAEAMRYYE"
     gene            2393..3865
                     /gene="lnt"
                     /locus_tag="PLU28_04315"
     CDS             2393..3865
                     /gene="lnt"
                     /locus_tag="PLU28_04315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HOG34323.1"
                     /translation="MSYVLTLLSGIMTGLSMPGNLFSFMIWGSLVFYLKAMASSQKTL
                     QRFLHTFIFSFSMLFTTFWWEIPVLSKNIPQVLGKYPAILGFFGFLGMILLMSLPYFL
                     IWLFSELYHRKSRRFNYWSLVLFYSFSYTAAEALREFGDLAFTGGALGYALYDHTGLL
                     QIASVFGYLGLTFLIVFVNSLIAFEKPKEALTKMLFIICAVYGLNFAIERFVPMPEHT
                     QSIKVDAIQMNVPQEVKYGSNSWTNYFMFQDLIKSSADSDSDVIVLPESAFMSDINDS
                     EIATALSADIAFTKKDVIMGYPRMTPDKDYNTVWHYDSSGSIKEYYDKVKLTPFAEFL
                     PYEVIFGKAQVFKLLRFYTAGDTYSVFDINGRKIGAQICFETYFPEVSMNQSINGAQA
                     LIVITNDGWFGTHTALMQHFSQIVFRAVENRKEVLQVANTGLTGKVDSYGRIIKTLKT
                     SQVNKVQFELNLNEKKTFFQSFALLLKILLFIVCLVLAIV"
     assembly_gap    4041..4140
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4141..5174)
                     /locus_tag="PLU28_04320"
     CDS             complement(<4141..5174)
                     /locus_tag="PLU28_04320"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOG34324.1"
                     /translation="MKRMILFVFIAYIAVFLTAQPQTSADILYEQQKKKMESLMNNQT
                     QVQQNILSNADKYFKEQVEKYELEYQKRMEEHNKKVKEAWGKFITFSTKEWGSFSQDY
                     KTYSRVSFDEEKGGFIEVKTIVTKEEGKKVAQENIKKQMNAILEEKDEYTGKPILEGD
                     VEKSQTEKGISNPTVSQQSDGKMIYTVKVPLVDDYAKRNAQKYKSLIQAVAKVLQLPA
                     ELVIAVVHTESSFNPRAQSASGAFGLMQLIPKYGAADGYYYVHKEKKLLTQDELFDPS
                     VNIKYGSAYLKAISSLYFSNISDEVKKEYLVIASYNMGPTAIKNLFKNVDINSVSSDY
                     VYNYIKTNAA"
     gene            complement(5187..6713)
                     /locus_tag="PLU28_04325"
     CDS             complement(5187..6713)
                     /locus_tag="PLU28_04325"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG34325.1"
                     /translation="MGYTSFLEKRYDEYFETFFSLCMTPMLFICQDGKVGCFNHPASE
                     FFGFSDEKGLSDLYFWEDPNYRFKKSLVQPCENKTMFDLAYSFESVKEEGFYKTSKEG
                     IVKARIIISQIVFSQELKGQLVEFHNLSGIYDGQEYMDTEKQMLELVFNSMTEGLALH
                     EIILNERGQAYDYRFLQVNPSFEKIMGLKSADITGKTVRQILPNVEQYWIDTYAKVAL
                     EGETMRINNFSSDLKKYFDVITFSPKKYYFAVLALDITEKIVMQRELIKAKEEAEAAW
                     RAKNIFIANMSHELRNPMNGIMGFVDLLCESELNDSQREYVNIIHDSSFSLLRILNDI
                     IDFSRLDSSAMSICEEMTDIRAYMSRIYEFFLPGCSLSGNSFVLETDSMVPRSIFMDK
                     ERYRQVISNLIGNAIKFCRNGKVEVKLSLISKTKEVCEINFSVKDSGIGIKQEDMHKL
                     FRPFSQIDSTPGKKYSGTGLGLAISQRIIQAFGSEIKVDSLPGEGSIFHFILKFKYKD
                     "
     gene            complement(6765..7976)
                     /locus_tag="PLU28_04330"
     CDS             complement(6765..7976)
                     /locus_tag="PLU28_04330"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOG34326.1"
                     /translation="MEKLFFEKDQLLYDVGQRLNYIYFIEEGYAVINFNGKEFPLCAS
                     TLGEWALADLESCERVMVEKGTVLLRYELSELSSRDCMSGISFFNMLCSVTQRLNLMN
                     SALCEISDSEQSVKIFKQNNPSYSYYYENKVFSRMLNVKSLYRMMCYEEAYNELLNIQ
                     AMDMQDDYSKEIMLWKVIILSHIDYAKASEMIKENFISDARGFGREYLEYRIKKEKSF
                     IMDLYGKNYMHFPGRSIIIAEGEKCDGNCFMLLHGGLKVSRFPGKEASFFSTIDEGEV
                     FGESSLISELPRQAMVYCEYPCDILVLSSSTVRNTIDSSCDFGIRLIKNQLQRIRKNY
                     IINQSRMITSQDERISTLLKLFGEKFVKAGVNKAEFSSISNTSLSETEKYMKGSGFLF
                     GSDGILANESH"
     gene            8168..9511
                     /gene="glmU"
                     /locus_tag="PLU28_04335"
     CDS             8168..9511
                     /gene="glmU"
                     /locus_tag="PLU28_04335"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HOG34327.1"
                     /translation="MKTLILAAGKGTRMKSKYPKVLHKILNKPIINWVVDTAALFSQQ
                     IGVVLGFGAQDVQKVLPPGVLVFEQKEQLGTGHAVMCAEEFLDSGELLVLYGDVPLIS
                     SATLNLLIEKHRKEKNDATILTFELDNPTGYGRILKQNGKFVKIIEEKDASEQEKRIC
                     ELNSGIAVYDCEKLKLALKKISSDNAQGEYYLPDVFLFFKKVGLLKTDDPVEVSGIND
                     RVQLSGMQAHARERINKALMLQGVTITDPLCTYIGPDVKIGSDTVVHPQSYIFGKTFI
                     GQDCEIGPMSVIYDSEIGDNVKVIRSEMDKTSVFHGASIGPFARMREGTVIEENVKIG
                     DFVETKKTTLKKNSKAQHLAYLGDATIGENTNIGAGTITCNYDGVNKHKTFIGDNSFI
                     GSNSSIVAPVNIGKNSIVGAGSVITKDVPEYSLALGRARQENKDGWVNKKNKKGD"
     gene            9515..10468
                     /locus_tag="PLU28_04340"
     CDS             9515..10468
                     /locus_tag="PLU28_04340"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HOG34328.1"
                     /translation="MSIVGRSEMKVFSGNSNLPLAQKIVEYIGSRLGDCELSKFANGE
                     INVRINETVRGYDVYVIQSISEPVNDNLMELLIMIDALKRASSGSISVIIPHYGYARQ
                     DRKAKGRDPISAKLVANLLTSAGADRIVTIDLHSEQIQGFFDIPVDNLWSFPVFAEHF
                     SNCKDIDLNDVVVVSPDVGGVKRARKFAEKLSTPLAILDKRRPKDNVAEIVNVIGDVR
                     DKTCIMFDDMIDTGGSLIESSQALEKNGAKKIIACATHGILSKDAKDRLQKSNIHKVI
                     ITDSIKHNDLPEKFEVLSVAPLFGEAVVRIRKNLSVSILFK"
     gene            10518..11189
                     /locus_tag="PLU28_04345"
     CDS             10518..11189
                     /locus_tag="PLU28_04345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HOG34329.1"
                     /translation="MARAYTLEVVNRDQSKKAKVLRRESMVPAVVYGPKLEKNFVVAI
                     EYQKVTALLEKALETSLVGLVFKNEKGVNEEHEAFIKSVQRDKTTDKVIHIDFYVPAN
                     DRKMTINIPFRFTGVPAGVTKGGVLQTPHHNITVELYPSDVLETITVDVSAMNINDHK
                     TVADIIPLLPHSAKVHMEETEVIAAVTKQVIVDTTAEGAVEEGAAAPGDVPATAQKSE
                     KKEEK"
     gene            11186..>11280
                     /locus_tag="PLU28_04350"
     CDS             11186..>11280
                     /locus_tag="PLU28_04350"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-tRNA hydrolase"
                     /protein_id="HOG34330.1"
                     /translation="MKKLIVGLGNPGSRYVFNRHNVGFLCVDRYY"
BASE COUNT         3432 a         2235 c         2065 g         3448 t
ORIGIN      
        1 aaaattggat gtgaaaaaag gtgacagtat agagcttttg gattcatcta atccacttta
       61 catacttctt ccaccgactt ttacagttgt agatgttctt tcggaaaaag gtctttttaa
      121 ctatcgggag gaaggcagtt ttatttctgg cgccatattt gtgagtcttc cgacaaccag
      181 gagatttgcc aaactagacg atggttactt ttctcatata atcatttcca acaccggcga
      241 cagtcttaag ggtgaaagac tttccgatag aataagacag gtggctgaaa agttttctac
      301 ttccttgcct ggaaaaaata ctacccagcg ctacctgaaa agcgagcaac tgcgtgctgt
      361 aacaggaaac ggcatatcat tggtatttat aatactgagc tcttttgcca taatagccgg
      421 agcagttctc ataaccaata tgtacgatat gcttatacag gaaaggaaaa gcgaacttgg
      481 aataatgcgg gccattggtt ttacaagaag ctggatacga aaggtaatgt tctatgaagg
      541 cttcttttat tcgttatttt ctcttccggt aggtattgtt tttggaacct tggtttccct
      601 gttcaccttt tcaaaagtga acgttcttgg agaacagttt tcaacttttg aatttacatc
      661 cggttccatt ccggtaatga gctcttacag catctcttta caatcaatga taacaggcgg
      721 tattctaggc atgatgatac ctttgcttat aatagtgctg gggtcatcaa aaattggaag
      781 attaaatatt tcagattcta tcagaaatat aagcgacttt cgtgaaaaac agaaaaaact
      841 ggaattcttc ctttggtttc tgatgataat aagcatacct ttcatttttt tcaaaaatcc
      901 atccataaga tatgcagccg tttcaatatt tatttttatg cttccttaca cggttcttaa
      961 aaaaatctca aaatactctc ttaatctttc agtactggcg gttatagcct ttactcttgc
     1021 cttcaggagc gagggtatca ttttggcagg tcttaaggcg cttatagtcc ttattgcagt
     1081 tattctattg tttgtttata accttaagcc tctggaaaga attatcactt tcttaataaa
     1141 gctgataacc cgaaaatcta ccagagctac cccgataatg agaattgctt tttcatatcc
     1201 gtcctttaac aaaaaaagaa cttctcttac caccgtactt tacggcatgg tcatttttgt
     1261 aatagtcata atcaccataa taccttattc tcagactcaa aggcttgcga aaagccgtaa
     1321 tgagcttttg ggaggaaatg acggttttgt cataagtctt ccttttcttc cttcacgtaa
     1381 ccgccctgat gtttcaaaaa tctcggaaac gcagggaata tcaaatgtat cgcagttcgg
     1441 attcgtaatg acacaagata gtgaaggtga aagctatcct cttattttac tctcagatga
     1501 tctcatcgac agttctcata tagattcaaa gttcagcagc atcagcagcg agttcaaagc
     1561 actttctgaa cgtgaagtgt ggaatctttt aaagacccac agcgattata ctattgtaaa
     1621 gagtctgcca ggttcaaaat taaaggttga tcagagtatt tctcttaaag taaacgaatt
     1681 tgatttcaat tctgcctccc agcttaagcc gtcagatacg gcacaaacaa tcaatctgag
     1741 agtaatagct atcgtttcgc aagccaataa ctcaccgata gtcggtccgg tggtaagtga
     1801 acggtctgtg atgggaatga tagaccaaag taagataata acgggctttt tattcaaggt
     1861 atctgatact tcaagagaaa cctataaaaa catttccaac tccttcaaaa tcaacgacta
     1921 tctttttatt tttgtagatg atataataga ttttgtaagt aagattactg ccggagcagt
     1981 agatatttta agctctttcc tttattttgg gcttgtagtt ggaatactag gcatatccgt
     2041 tacgacaata aaatctatcg aagaacgcag aaatgccata ggcatgctta aaagcatagg
     2101 ttttacatca cgtatggttt tctggtcctt ttttctggaa acaagcttta taatagtaat
     2161 gggaatcctt atcggtgtac tagccggaag cattgcaagc tatgaaatat atacccagat
     2221 tctgaaagat tctgctcagt actttatgat accctactca aaacttttag gtatgtctgc
     2281 ggtattttat cttatctcaa ttattttcac ttattttccg tgcaggcagg cttcaaagat
     2341 atcacctgca gaggctatgc gttattacga ataaacttac gggagtggta taatgagcta
     2401 tgttcttact cttttatcgg ggattatgac cggtctatca atgccgggaa accttttttc
     2461 tttcatgata tggggatcgc ttgtttttta tcttaaagcc atggcatcct cacaaaaaac
     2521 cttgcagaga ttcctgcata cttttatctt ttctttttca atgttgttta ctactttctg
     2581 gtgggagatt ccagttcttt caaaaaatat cccgcaggtt ctaggaaagt atccggctat
     2641 attaggattt tttggttttc ttggaatgat acttcttatg agccttcctt actttcttat
     2701 atggcttttt tcagagcttt accatagaaa aagccgtagg ttcaactatt ggtcgcttgt
     2761 actgttttat tcattttcat ataccgctgc cgaagctttg agagagtttg gagatctggc
     2821 tttcacaggc ggtgccttag gatatgctct gtatgaccat accggtctgt tgcagattgc
     2881 aagcgttttt ggatatctag gacttacatt tttaattgtt tttgtcaaca gtctcatagc
     2941 ttttgaaaaa cccaaagaag cacttactaa aatgctcttt ataatatgtg cagtatatgg
     3001 tttgaacttt gccatagaac gttttgtacc catgccagag catactcaaa gtataaaagt
     3061 tgatgccata cagatgaatg ttcctcagga agtaaagtat ggttcaaact catggaccaa
     3121 ctactttatg tttcaggatc tcataaaatc atccgcagac agcgacagtg atgtcatagt
     3181 gctaccggaa tcagcgttca tgtcagatat aaacgactcg gaaatagcaa ccgctctttc
     3241 agcagatatt gcttttacaa aaaaagatgt gataatgggt tatccccgca tgacaccgga
     3301 taaggattat aacacagtgt ggcattacga ttcctccgga agtataaaag agtactacga
     3361 taaggttaaa cttactccat ttgcggagtt tcttccttat gaggttatat tcggaaaagc
     3421 acaggttttt aaactcttac gattttatac cgccggtgat acgtacagtg tttttgatat
     3481 aaacggcaga aaaatcgggg cacagatatg ctttgaaaca tatttcccgg aagtttcaat
     3541 gaatcaatcc ataaacgggg cacaagccct catagtaata accaacgatg gctggttcgg
     3601 gacacataca gcacttatgc agcatttttc gcagatagta ttcagagcag ttgaaaacag
     3661 aaaggaagta ctccaggtgg ccaacaccgg tcttaccgga aaggtagact cttacggacg
     3721 aatcataaaa actctcaaaa catcccaggt aaacaaggtt cagtttgagc ttaacctcaa
     3781 tgagaaaaaa acttttttcc aatcctttgc tcttttactt aaaatactct tattcatagt
     3841 ttgtcttgtg cttgccatag tataaaattt aaaaacgtcc ggcgcttaaa taagctcagc
     3901 cggacgttat tttaggtttt atttaaagaa ccctgagtac ccatccttcc tctttataac
     3961 cttttcaatg taatcctgcg tttctttggc tgcattggtc tttatataat tatatacata
     4021 atccgaagat accgagttta nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 ttggctgcat tggtctttat ataattatat acataatccg aagataccga gtttatatca
     4201 acgtttttaa aaagattttt tatagctgta ggtcccatat tatagctggc gataaccagg
     4261 tactccttct tgacctcatc gcttatattg gaaaagtaca aagaagaaat agctttaaga
     4321 tatgcagaac catactttat atttacagac gggtcaaaaa gttcatcttg ggtgagaagc
     4381 tttttttcct tgtgaacgta gtaatatcca tcggcagcac catatttagg tataagttgc
     4441 ataagcccga acgcacccga agcactttga gctctgggat taaacgaaga ttctgtatga
     4501 acaaccgcta tgacaagctc cgcaggaagc tgcaaaacct ttgcaacagc ctggataaga
     4561 cttttgtact tttgcgcatt gcgtttggca taatcatcta caaggggaac ctttaccgta
     4621 tatatcatct ttccgtcact ctgctgagaa acagttggat ttgatattcc tttttctgtc
     4681 tggctttttt ctacatcgcc ttcaagtatc ggctttccgg tatactcgtc tttttcttct
     4741 aaaattgcat tcatctgttt tttaatgttt tcctgagcta ctttctttcc ttcttctttt
     4801 gtgactatgg tcttcacttc tataaaaccg cccttctcct catcaaaact aaccctgctg
     4861 tatgttttat agtcctgtga aaaacttccc cattcttttg tagaaaaggt tataaacttt
     4921 ccccatgctt cttttacttt tttattatgc tcttccattc ttttctgata ctcaagttca
     4981 tatttttcaa cctgctcctt aaagtactta tcggcatttg aaagtatatt ttgctgaacc
     5041 tgggtctgat tgttcataag gctctccatc ttttttttct gttgttcata aagaatatct
     5101 gcagatgtct gtggctgagc tgtaagaaaa accgcaatat atgcgatgaa aacaaataaa
     5161 atcatcctct tcatatttta tctcctttaa tctttatact tgaacttcag tataaaatga
     5221 aagatgctcc cttcaccggg caaactgtcc acttttatct cacttccaaa tgcctgaata
     5281 atcctctgag atattgccaa acccagtcca gtaccgctgt acttctttcc gggcgtagaa
     5341 tcaatttgac tgaatggcct gaaaagcttg tgcatgtctt cctgttttat tcctattcca
     5401 ctgtccttta ctgaaaagtt tatttcacat acttccttcg ttttagaaat caatgaaagc
     5461 tttacttcaa cctttccatt tctgcaaaat tttattgcat tgcctataag attagaaata
     5521 acctgtctgt acctttcttt gtccataaaa atactgcgtg gtaccatact atctgtttct
     5581 aaaacaaaac tgtttccgga caaagagcag cccggaagaa aaaattcata tatcctgctc
     5641 atataggctc ttatatctgt catttcttcg caaatgctca ttgcagatga atccagacgg
     5701 gaaaaatcta ttatgtcatt gagaattctg agaagactga acgaagaatc atgaattata
     5761 ttaacatact ctctttggct atcattcagc tctgattcac aaagcagatc gacaaacccc
     5821 attataccgt tcataggatt tctaagctcg tgactcatat tggcaataaa aatatttttt
     5881 gctctccaag ccgcttctgc ttcttctttg gccttgataa gctctctttg cataacaatc
     5941 ttctccgtaa tatcaagtgc gagaacggca aaataatatt tttttggaga aaaggtaatt
     6001 acatcaaagt acttttttaa atcggaagag aagttgttta tacgcatggt ttcaccttcc
     6061 agggcgacct tggcgtatgt atctatccaa tactgttcga cattgggaag aatttggcgt
     6121 acagttttac cagttatatc agcacttttt aatcccatta tcttttcaaa agaaggattg
     6181 acctgcaaaa accggtaatc ataagcctga ccgcgttcat ttaaaataat ctcatgcaga
     6241 gccagaccct cggtcataga attaaaaacc aattcaagca tctgcttttc agtatccata
     6301 tactcttgcc cgtcatatat gccggatagg ttatgaaact caacaagctg acccttaagc
     6361 tcttgagaaa aaactatctg tgaaataata atccttgctt ttacaattcc ttcttttgaa
     6421 gttttataaa atccctcctc tttgacggat tcaaagctat acgctaaatc aaacattgtt
     6481 ttattctcac acggctgaac caatgacttt ttaaacctgt aatttggatc ttcccaaaaa
     6541 tacaggtcag aaagcccctt ctcatcggaa aatccaaaaa attcagaagc aggatggtta
     6601 aaacatccca cttttccatc ttgacagata aagagcattg gtgtcataca tagactgaaa
     6661 aaagtctcaa aatactcgtc gtatcttttc tccaaaaagc ttgtatatcc cataaaacct
     6721 ccggtattca aaggctatcg cctttaaact gacaataact ggatctaatg actttcatta
     6781 gccaaaattc catctgagcc gaagaggaag ccggaacctt tcatatactt ttccgtttca
     6841 gataaagatg tattggagat acttgagaac tcagctttgt ttacgcccgc tttcacaaac
     6901 ttttctccga aaagcttcag cagtgtagaa atacgctcat cctgcgaagt tatcattctg
     6961 ctttgattta taatatagtt ctttcttatt cgttgaagtt gatttttaat aagcctgata
     7021 ccaaaatcgc agcttgaatc tatagtgttc ctgacggtgg aactgcttaa taccagtata
     7081 tcacaaggat attcgcagta aaccatggcc tgtctgggaa gttccgaaat caatgaggac
     7141 tctccgaaca cctcgccttc gtctatggtt gaaaaaaacg aagcctcttt tccgggaaac
     7201 ctagatactt tcagtcctcc gtgaagaagc ataaaacagt ttccatcaca cttttcaccc
     7261 tcggcgatta taatgctgcg tccaggaaag tgcatataat ttttaccgta aagatccatt
     7321 ataaagcttt tttctttttt gattctatat tcaaggtact cacgtccaaa acctcttgca
     7381 tctgaaataa aattttcttt tatcatttcc gatgcttttg cgtaatcaat gtgggaaagg
     7441 attatcactt tccaaagcat tatctcctta gaatagtcgt cttgcatatc catggcttga
     7501 atattcaaaa gctcattgta agcctcttcg taacacatca tcctgtacaa agatttaacg
     7561 ttgagcatcc tgctgaaaac tttattttca tagtagtaag agtacgaagg attgttctgc
     7621 ttaaaaattt ttaccgactg ttcactatct gaaatttcgc aaagtgctga gttcataagg
     7681 ttcagccgtt gggtcacaga acaaagcatg ttaaagaaac ttattcctga catacaatcc
     7741 ctagaagata gttcagaaag ctcatatcta agaagtactg tgcctttttc aaccataacc
     7801 ctttcacatg attccaagtc cgccaaagcc cattcgccca aagtagatgc acaaagcgga
     7861 aactctttgc cgttaaaatt aataacagca taaccttctt ctatgaaata aatatagttc
     7921 agtctctgtc cgacatcgta aagaagctga tctttttcaa aaaaaagttt ttccataccc
     7981 accccacaga ctcggcatat taaaattata catcttaatg tgtatttttt ctcttttgaa
     8041 tttcaatata tcttaatctt tttttaccat ttaatgttac aaagaatggt ataataacta
     8101 tgtcatgcca taaatacttt gtggcaactg catcctgtaa atattaaaac gctttggggg
     8161 aatcgacttg aaaaccttaa tacttgccgc aggcaaaggc accaggatga aatcaaagta
     8221 tccgaaggtc ttgcacaaga tccttaataa acccataata aactgggtgg tggatacagc
     8281 agccttattt tcgcaacaga taggagttgt tttgggattc ggagctcagg atgttcaaaa
     8341 ggtacttcct cccggagtac ttgtctttga gcagaaagaa cagttgggta ccgggcatgc
     8401 agtaatgtgc gcagaagagt ttcttgacag cggcgagctt cttgtccttt atggcgatgt
     8461 acctctcata agctcagcta cactgaatct ccttatagaa aaacacagaa aagaaaaaaa
     8521 tgacgctact atacttacct ttgaacttga caatcctaca gggtatggaa gaatattgaa
     8581 acaaaacgga aaattcgtaa agataatcga agaaaaagat gctagtgaac aggaaaaaag
     8641 gatatgcgaa cttaacagcg gaatagccgt atacgactgt gaaaagctta agctcgcact
     8701 caaaaaaata tcttccgata acgctcaggg ggagtactac cttcctgacg tttttttgtt
     8761 ttttaaaaaa gttggtctgc ttaaaaccga tgatcctgtc gaagtatctg gtattaatga
     8821 cagggtacag ctttcaggta tgcaggcaca cgcgcgtgaa aggataaaca aagctctgat
     8881 gcttcaggga gtaaccataa ccgatcctct ctgcacttat ataggacctg acgttaaaat
     8941 aggttcggat acagtcgttc atcctcagag ttatatattc ggaaaaacct ttataggaca
     9001 ggactgcgag attggcccca tgagcgttat ttacgattcc gaaattgggg acaatgtaaa
     9061 ggttatccgt tctgaaatgg ataaaacatc agtttttcat ggtgcaagca taggtccttt
     9121 cgcccgcatg cgtgaaggaa ccgtaataga agaaaatgta aagataggcg attttgttga
     9181 aaccaagaaa accactctga aaaagaactc aaaagctcag catcttgctt acctgggaga
     9241 tgccaccata ggggaaaata ccaatatagg agcaggaacc ataacctgca actatgacgg
     9301 agtaaacaag cataaaacct ttataggcga caactctttc ataggaagca acagttcgat
     9361 tgtagctcct gtcaatatag gtaaaaactc aattgtcggc gcaggctccg tgataacaaa
     9421 ggatgttccc gagtattcac ttgcattagg ccgtgcgcgt caggaaaaca aagacggttg
     9481 ggtaaataaa aaaaataaga agggagacta agccatgtcg attgtcgggc gatctgagat
     9541 gaaggtattc agcggtaatt caaaccttcc tcttgctcaa aagatcgtag agtacatagg
     9601 ctccagatta ggagactgtg aattatccaa gtttgccaat ggtgagatca acgttcggat
     9661 aaacgaaact gtgcgaggat acgatgttta cgttatccaa tctatatccg aaccggtaaa
     9721 tgataacctt atggaacttc tgattatgat tgatgctctt aaaagagctt catcaggatc
     9781 tatttcagtg ataatacctc actacggcta tgcaagacaa gacagaaaag ccaaagggag
     9841 agacccaatt tccgcaaagt tggttgccaa tcttctcact agtgcaggcg cagaccgtat
     9901 cgttactata gatctgcatt ccgaacagat tcagggtttc tttgatattc ccgtggataa
     9961 cctctggagt ttcccagtct ttgctgaaca tttttccaac tgcaaagaca ttgatcttaa
    10021 cgatgtagtt gttgtttctc ctgacgtagg aggagttaaa agagccagaa agtttgctga
    10081 aaagctttcc acccctcttg ccattctgga taaaagaaga cccaaggata atgtggcaga
    10141 aatagtaaac gttattggag atgtaagaga taagacatgt ataatgtttg acgatatgat
    10201 agatacaggt ggttctctta tagaatcctc acaggcgctt gaaaaaaacg gagccaaaaa
    10261 gattatagcc tgcgcaaccc acggtattct ttccaaagac gcaaaagaca ggctgcagaa
    10321 atctaatata cacaaagtca taataacaga ttcaataaaa cacaacgatc ttccagaaaa
    10381 atttgaagtg ctgtccgtgg cacctttgtt cggagaagcc gtagtacgga taagaaagaa
    10441 tctttcggta agtatccttt ttaaataata gtattacgtc cctaaaggga aaaaataaaa
    10501 acaggaggtt gaagacgatg gcgagagcat atactcttga agtagtcaac agagatcaga
    10561 gcaaaaaagc aaaagtatta agaagggaat ccatggtacc agcagttgtt tatggtccta
    10621 aactcgaaaa gaattttgta gtggccattg aataccaaaa ggttacagct ttacttgaaa
    10681 aagcacttga aacttctctg gtaggtctgg tatttaaaaa tgaaaaaggc gttaacgaag
    10741 aacacgaagc atttattaaa tcggtacaga gggacaagac aaccgataag gttatccata
    10801 tagattttta cgttcctgct aatgacagaa agatgactat caatattccg ttcagattca
    10861 caggagtgcc tgccggagta accaaaggcg gagtgcttca gacacctcac cataacatca
    10921 cagttgagct ttatccctcg gatgttttgg aaaccattac agtagatgta tctgctatga
    10981 atataaacga tcataaaacc gttgctgaca taattccatt gcttccacac agtgcaaagg
    11041 ttcatatgga agaaaccgaa gttattgcag ctgttacaaa gcaggttatt gttgacacca
    11101 ccgccgaagg cgcagttgaa gaaggtgccg cagctcctgg cgatgttcct gcaactgcac
    11161 agaaatctga aaagaaagag gaaaaatgaa aaaattaatt gtcggtcttg gcaatcccgg
    11221 ctcacgttat gtctttaaca gacataacgt gggctttttg tgtgtggaca gatactatga
//
LOCUS       DAOSHR010000054        11147 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_4929_length_11147_cov_5.204652, whole genome shotgun sequence.
ACCESSION   DAOSHR010000054 DAOSHR010000000
VERSION     DAOSHR010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 11147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11147
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4929_length_11147_cov_5.204652"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="PLU28_04355"
     CDS             complement(<1..516)
                     /locus_tag="PLU28_04355"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOG34331.1"
                     /translation="MKRKHKPLILLAVIIVATLYIFFIEPSLTIAVNRIKINRFNSSD
                     TLKIIQISDLHIDYYYFFHDNILSRVLMEHPDIVIFTGDALTRSSSFGALAEFFSRLS
                     NIAPVYAIYGNWDYYDKEKIDSVYRVCHVGNIEAEQLRLKTKNKKITFTSLPMYESPG
                     QYYFDKGEYNIF"
     gene            566..1015
                     /locus_tag="PLU28_04360"
     CDS             566..1015
                     /locus_tag="PLU28_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34332.1"
                     /translation="MRKSLFFVAAVLLTNLILFIFKQSIKDFIYINVLFFAGIYDYYK
                     YIIPETAVITIIVLSIIDFRAENLLFSLVAFFSLLIFYRQRKIGFGDIQLISAIFFLN
                     GYEALFILAIACVLMIILNFRKDTIRIPMGFYLMLGATAFFISDILI"
     gene            1021..1791
                     /locus_tag="PLU28_04365"
     CDS             1021..1791
                     /locus_tag="PLU28_04365"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HOG34333.1"
                     /translation="MQIIFLKILSSFFEVPGIFITLCIVMFFYYLKKEKKIRKFLLII
                     TLIVYIISSGWFARIMVYPLENMYPPFSTQGKMFSKNGLLVVLGGGMNSNVPSDPTGD
                     LSEISFQRIYNGFLLYQNLKYPIVVTGGKLPNTNDSPEALKMKETLVRLGVNPSDILL
                     EPKAVNTSENAIYSAEKAKEAGAEKVYLITSAIHMPRSFSIFKKYMKDLEVVPVPVGY
                     LLSRNSLNWYDFFPSISSLRAVSMAWHEYAGMIFYITK"
     gene            1849..1925
                     /locus_tag="PLU28_04370"
     tRNA            1849..1925
                     /locus_tag="PLU28_04370"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1883..1885,aa:Pro,seq:ggg)
     gene            complement(1962..3254)
                     /locus_tag="PLU28_04375"
     CDS             complement(1962..3254)
                     /locus_tag="PLU28_04375"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HOG34334.1"
                     /translation="MNKYLKNILEANKELIGWKLKDMQTQSTEVFLVRKNINMIRNKK
                     VHDMELTVYTESENGKYIGSASIFLYDTMTEPEIKDAVNDAIQSSKLVKNEYYSIAQP
                     QEFIPVKLDSYFDHKNNEQAVKDIMDALYFKDTRQDVYINSCEIFLEKTQKILFNSKG
                     IEYREDFNKCYIEYVITASSNGDEIELIDKMEFSDFNKTFIENMIEDKFEIAMMRLKS
                     VNTPNLVSCDVILRDDAVKDFFGYYIYKTNAMAIYEKTSDFMVGKDAIKNKLSDKLSI
                     MLDPYIAGSTFSSQFDDDGVLLKKTQIIKDGVVEKIWGNLRFSQYINTQCTGNISNFC
                     VASGKSSYKEMICGKVLEIHAFSDFQLDEVTGDFGGEIRLAYLHEDGGMKVLTGGSIS
                     GNINFEECSVILSEETVQINNYFGPKYIKLTNVSVTGE"
     gene            complement(3264..4688)
                     /locus_tag="PLU28_04380"
     CDS             complement(3264..4688)
                     /locus_tag="PLU28_04380"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOG34335.1"
                     /translation="MKIELSNFLDSSCSKMKEIISILKKNFDYVSVLGTDCHGKQYRV
                     NRKSTGVADSRWNERGFVIRAVKDGFYYEFSTDSITDAVQVADDFSKNSDRAAYFETE
                     SFKIKRLPIIEEEKAQADFRGEILLNPDECMASQKILDSMNNIKNTIVAVSEKIINCE
                     VVYEHVNVSKIFVSDKKILSQSYMWSQGYLFVYAAQDDSIKYNYDTFSGLSGMELLNE
                     MGNRCEDTAHTALKLLQSERIKPGEYEILCSPEVAGLIAHEAFGHGVEMDMYVKNRAK
                     SVEYMNKEVASALVTMHDGAASAKHVSSYFFDDEGVLAQDTVIIKNGILINGLCDQVS
                     ALELGVIPTGNGKRESFRRKAYTRMTNTFFSKGKDKFEDMVKSIKHGYYLEKEESGME
                     DPKNWGIQCVVLYAREIRNGRLTDKYVSPIIMTGFVPDLLKSISMVSDDFGLSGSGAC
                     GKGYKEFVKVSCGGPCIKARAKLG"
     gene            4839..5264
                     /locus_tag="PLU28_04385"
     CDS             4839..5264
                     /locus_tag="PLU28_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34336.1"
                     /translation="MKKFLYLFLSVFMVFFLFSCSSQSAFQNTQNALPGTPEKIRFTV
                     SFIVTVPQNTDYVYIMGDFNNWNPGDEEYRLKKIDSVTYSITLSQEKGKTINFKFSKG
                     TLFSVETDSNFYETDPRTFRFDYNNDEVRFEVENWLDNK"
     gene            5351..6331
                     /locus_tag="PLU28_04390"
     CDS             5351..6331
                     /locus_tag="PLU28_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HOG34337.1"
                     /translation="MKKVAVITTGGTIAMAHDPNMGVILSENKNKELSEIPQIHQIAD
                     IEFHEFFDVPSPYITPGMMFELSKYVKKILSRSDIDGVVITHGTDTLEETAYFLDLTI
                     GSEKPVVVTAAMRNWSEPSTDGPMNLIASVKIASCDLSVDQGVMVCLNDEIHAAREVT
                     KTYTSNVATFDSPGYGPLGIVDEDTVVFFRRSLERMYIQTDRIEDRVALIKTYTGDNG
                     AIIKTLPDMGYKGVVIEGFGRGNVPPAVADAMEYLLEKNIPVVVVSRCFKGRVLGVYG
                     YIGGGKDLKERGIILGQEVSGQKARIKMIVVLGKTKDTESLRRYFEFQNR"
     gene            6343..7353
                     /locus_tag="PLU28_04395"
     CDS             6343..7353
                     /locus_tag="PLU28_04395"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOG34338.1"
                     /translation="MIKNQKAGLFTGIYILLFFSIAIISMKIFAILVLSLGSILVIDL
                     FAKVLSKWFKFRRVVSLTLSMIFIFSIFVIAFVFLIPAVIKEIDTFVVFIDRFFVNRE
                     WEKFFTNIFPDTEKTLSNFMVSLQPKLLEFLNSFVGKLPAYGQQIALFLAYYFLLTLY
                     GIYNIDSLKKCIGYLYPRSIRSTGEKFFSELYHSLDSFVFSTFFSAIFVGVCAFFIMS
                     FLGIKYTVLMSFWAFVTNFIPIVGVIFEFVPLALVGLSSGVESMVWLLVLMSVIHGSA
                     FIFFLFVMRGYSKINPFFIILAILIFGQIFNVLGILIAVPLCLIIKLFWTNFIKPYFE
                     RG"
     gene            7361..8752
                     /locus_tag="PLU28_04400"
     CDS             7361..8752
                     /locus_tag="PLU28_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34339.1"
                     /translation="MNFRKTVIFLLCFLFFSMSFGLTVEEIYNLSKLPQTLEKSIDEF
                     LKYFYENPADPQLEVYGSLIYSKKTIRDKYPSYAFVNGLISENSNDFLMSLSAMSPSF
                     KIPPEDTQLVVMILKDLIPKLQKIFETGTLQEASLKHSYHLDGINLMVTPKTYKPFLE
                     FVVFKTLNTPSFLDDDMKKFIEFFVPRNILSTINAIISSSRLVLNESDYIGGYVLLDY
                     INTLGMIDSTNIDSFVTLKKYYTVRNSITKLSNSVFMVDDQELQVYLDDLYTQIEELS
                     TLSSSKKSIEEILLLVLKTLNTRLDKNTKKLFSKRYDFDKVLTSFPDNIQNELLRLKL
                     KIDEFYISAQVKPDQTKVSTGATSSSGTEMKTYTFFYFLGTVIFLVTAFLFLTMEFFP
                     NVQKIEFLCKIGMGRYAIHLAEKLVIKNANDFRSFFALAKALEVCGRYEDSINAYKTA
                     MSIREKNHENKGE"
     gene            8782..9423
                     /locus_tag="PLU28_04405"
     CDS             8782..9423
                     /locus_tag="PLU28_04405"
                     /inference="COORDINATES: protein motif:HMM:NF013012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34340.1"
                     /translation="MKKVYILLIKGKFYDFNTIDSKNSGSKLAYEVDNILKKNAISVS
                     DMDTFGIDTGPGSFTGIRIGISFLSGLLAIKNDMEIKNIISTDIINESNTSTISNFAV
                     LRRAREDAAYVCIYRDRVLAGQPNMIKGSELAYVLKGLELTGEESRYFIDKFSLGNTF
                     FETEVTPQGMMKAFMNSKPVKLNDLKPLYLQKPLAVEIWEEKNKILMNKEFWN"
     gene            complement(9455..10087)
                     /locus_tag="PLU28_04410"
     CDS             complement(9455..10087)
                     /locus_tag="PLU28_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HOG34341.1"
                     /translation="MLKIPKPTVKRLAIYYRCLAKLKEDNVEKTSSQEIAYILGIKAS
                     QVRKDLSYFGEFGKRGVGYDVDKLSFKLREILGLNKSWNVAIIGAGNLGNAIANYSGL
                     EKNGFRVVAAFDNDEKKIGKYISTNVKIYKMEDLEEIIRDNLIEIAILTVPAQAAQNV
                     SELVIKKGIKGILNFTPATITVPLNISVENVDFVVSLKSLTFALGFLFEK"
     gene            complement(10177..10788)
                     /locus_tag="PLU28_04415"
     CDS             complement(10177..10788)
                     /locus_tag="PLU28_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34342.1"
                     /translation="MKLRIILIVLAVSALILSIGTEIRMVMLKKQFQSDINRLSGTIN
                     TRVTNIENNMEKVDTYFKPNGMVEKYIVANSFLKKQMDDLEYIITSMQKSDKSGYMQI
                     YITGSKDVWCYFKNSAGNYVFQSNLQPGLNVYKFFFFKQAEIQTKYTIDVPYDASFNA
                     GDPTKVFFLINEPGQTRLKAYPQTGIANLYKDLNLYIPTVTGK"
     gene            complement(10785..>11147)
                     /locus_tag="PLU28_04420"
     CDS             complement(10785..>11147)
                     /locus_tag="PLU28_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-70"
                     /protein_id="HOG34343.1"
                     /translation="SFITYLNRQKNKVLSEALSIENTFEDSNDDAESFFEQPCEQDMS
                     KFILIRYLIEQEVSLMQDYERVIIYDWFDGFSYKEIAEKHSIAFKKVDNTIQKIKGLL
                     SKKTELIERIDDFLGGKI"
BASE COUNT         3421 a         2082 c         1832 g         3812 t
ORIGIN      
        1 aaatatgtta tactctcctt tgtcaaaata atactgtccg gggctttcat acatgggcag
       61 gctggtaaaa gttatttttt tattctttgt tttcaatcga agttgttcag cttcaatatt
      121 acccacatga cacactctgt ataccgaatc tattttttcc ttgtcatagt aatcccaatt
      181 tccatatatg gcataaacgg gagctatatt tgaaagcctg ctgaaaaact ctgcaagtgc
      241 accgaaagac gagctcctgg taagtgcatc gcctgtaaag attacgatat cgggatgctc
      301 cattaaaact ctggacagta tattatcgtg gaaaaaatag taataatcaa tatgcagatc
      361 acttatctga ataatcttca aagtatcaga gctgttgaac ctattaatct ttatgcggtt
      421 gacagcaata gtaagcgatg gttcaataaa aaaaatatat aatgttgcta ctataattac
      481 agcaagaagt ataagtggct tatgttttct tttcataagg cctccggtta ttgcaaaaac
      541 tttctttcta tgataaaatg aaattatgag aaaatcatta ttctttgtgg cagcagtgct
      601 tttaaccaac ctgatacttt ttatctttaa acaatcaatc aaagatttta tatatataaa
      661 tgttttattt tttgcaggaa tatacgacta ttacaagtat attatacctg aaacggcagt
      721 aattacgatt atcgttttat caattattga tttcagagct gaaaatcttt tattttcgtt
      781 agttgctttt ttttcgctac ttatatttta cagacagcgt aaaattgggt tcggagatat
      841 acagcttata tcggccatct tttttttaaa cggttatgaa gctcttttca tactggcaat
      901 tgcatgtgtt cttatgatta ttttaaattt cagaaaagat actatccgta tacccatggg
      961 gttttacctt atgctcggcg ctacagcgtt tttcatctct gatatactaa tttaagggag
     1021 gtgcagatta tttttctgaa aatactttct tctttcttcg aggtacctgg aatatttata
     1081 accttgtgta ttgtaatgtt tttttattac ttaaaaaaag agaaaaaaat ccgaaagttt
     1141 cttttaatta taactttgat cgtatatata atttcaagcg gctggtttgc aaggattatg
     1201 gtttatcccc ttgaaaatat gtatccgcct ttttcaaccc agggaaagat gttttcaaaa
     1261 aatggactgc tggtagtttt agggggagga atgaattcaa acgttccgtc agatcccacc
     1321 ggtgatcttt ccgaaatatc ttttcaaaga atatacaatg gttttcttct ttatcagaac
     1381 cttaaatatc ctatagtggt aacaggggga aagcttccca atactaacga ttcgccagag
     1441 gctcttaaaa tgaaagagac actggtaaga ctgggtgtaa acccttctga tattcttctg
     1501 gaacctaaag cagtaaatac atcagaaaat gctatatatt ccgcagaaaa agcaaaggaa
     1561 gcaggagctg aaaaggttta tcttattact tcagctatac atatgcccag atctttcagt
     1621 atttttaaaa aatatatgaa ggatctggag gtagttccag tgcctgtagg atatctctta
     1681 tcgcgcaata gtctgaactg gtatgatttt tttccaagca tatcctcatt gagggctgtt
     1741 tcaatggctt ggcacgagta cgcaggcatg atcttttaca taacaaagta gtttattgct
     1801 gttgattttt aacatttatt atgatagaat aatcttgtgt ttgtttgacg gagcgtggcg
     1861 caggttggta gcgcgtttgc ttggggtgca aaaggtcgct ggttcaagtc cagtcgctcc
     1921 gaccaaaaaa ataaagtccg gcatatgccg gactttattt tttattcacc tgttacagat
     1981 acatttgtaa gcttaatata cttaggaccg aagtaattgt ttatctgaac agtttcttca
     2041 gaaaggataa ccgagcattc ttcaaaatta atattgcccg atatagaacc gcctgtaaga
     2101 accttcatac caccatcttc atgaagatac gccagtctta tttcgcctcc aaaatctccg
     2161 gttacttcat ctaattgaaa atctgaaaat gcatgtattt caagtacttt tccacaaatc
     2221 atttctttat aagagctttt ccctgatgct acacagaagt tacttatatt tcctgtacac
     2281 tgggtgttta tatactgtga aaatcttaaa ttaccccata ttttttcaac cacaccatct
     2341 tttattatct gagttttttt caaaagtact ccatcgtcat caaactgcga agaaaaagtt
     2401 gaaccggcta tgtatggatc caacataata cttagtttat cggagagctt gttttttata
     2461 gcatctttcc ctaccataaa atccgatgtt ttttcataaa tagccatagc gttggtttta
     2521 tatatatagt atccaaaaaa atcttttacc gcatcatccc tgagaattac gtcacaggat
     2581 acaagattgg gtgtattgac actttttaat ctcatcatag ctatctcaaa cttatcctca
     2641 atcatatttt caataaacgt tttgttgaag tctgaaaact ccatcttatc aatgagttca
     2701 atctcatcgc cgtttgagct ggctgtaata acatactcga tgtagcattt gttaaaatct
     2761 tccctgtact ctattccttt agaattaaat aatatctttt gagttttttc aagaaatatt
     2821 tcacaggaat ttatataaac atcctgtctt gtatctttaa agtacagtgc atccatgata
     2881 tcctttactg cctgttcatt gtttttatga tcaaaatatg aatccagctt tacaggaata
     2941 aactcttgag gctgggcaat actgtagtat tcgtttttta ctaattttga ggactgtatc
     3001 gcatcattta cggcatcttt gatctcaggc tcagtcatag tatcatacag aaatattgaa
     3061 gctgagccta tatattttcc gttttcactt tcagtatata ctgtgagctc catatcgtga
     3121 acctttttat ttcttatcat atttatattt tttcttacaa gaaatacttc tgtagactga
     3181 gtctgcatat ctttaagttt ccagccgatc aactctttat tagcttcaag aatatttttg
     3241 agatatttat tcatcttatc ctcctatccc agtttagccc tagcttttat acaagggcca
     3301 ccgcacgaaa cctttacaaa ctccttatat ccttttccgc atgctccaga tccggaaagt
     3361 ccaaaatcat ccgataccat cgatattgat ttgagaagat ccggaacaaa ccctgtcatt
     3421 attataggag agacatactt atcagttagt ctaccgtttc tgatctccct tgcataaagc
     3481 accacacact gtattcccca gtttttagga tcttccatac cgctttcctc tttttcaaga
     3541 taatagccgt gctttatgct ctttaccata tcttcaaact tatcctttcc cttggaaaag
     3601 aaggtatttg tcattctagt atacgctttg cgcctgaaag attctctctt tccattgcct
     3661 gttggtatta ctccaagttc aagtgcggaa acctgatcac ataagccgtt tatgagaata
     3721 ccgttcttaa tgattacggt gtcctgggca agaactccct catcgtcaaa aaaatatgat
     3781 gaaacatgtt tggcgcttgc tgcaccatca tgcatggtga caagcgcaga agcaacctcc
     3841 ttattcatat actccacaga ttttgctctg ttttttacat acatatccat ctcaacacca
     3901 tgcccaaagg cttcatgagc tataagacca gccacttcag gactgcaaag aatttcatac
     3961 tctcccggct ttatcctttc gctctgaaga agcttcaagg ctgtatgtgc cgtatcctca
     4021 catctgtttc ccatctcatt aagaagttcc atcccgctaa gcccggaaaa tgtgtcatag
     4081 ttatacttta tactatcgtc ctgggctgca tagacaaaca agtagccttg gctccacata
     4141 tatgactgtg aaaggatttt tttatcagat acaaagatct tggatacatt tacgtgctca
     4201 taaacaactt cacagtttat tattttttcc gaaacggcta caatcgtatt ttttatatta
     4261 ttcattgaat ccaaaatctt ctgtgaagcc atacactcat caggatttaa aagaatttcc
     4321 cctctaaaat cagcttgagc cttttcctct tcaataatgg gtagccgttt aattttaaag
     4381 ctttcggtct caaaataagc tgctctgtcg ctgtttttag agaagtcatc agcaacttga
     4441 actgcatccg ttatagaatc agttgaaaat tcatagtaaa aaccatcttt cactgccctt
     4501 attacaaatc ctctctcgtt ccatctgctg tcggccacac cagtactttt tctgttaact
     4561 ctgtactgct ttccatgaca gtcagttcca agtaccgaga catagtcgaa gttttttttc
     4621 aaaatactta tgatttcttt catttttgaa caggaactgt ccaaaaaatt tgaaagttca
     4681 atcttcatgg ttcaccccca ttctctgttt ataaaaatat agcacttaat ccttcttttt
     4741 acaagtgact ttattcccct ttaatctcta tataaaacag atattacact gttttttaat
     4801 aattgtgtgg tatcatgaaa tcaaaaggag ggattttttt gaaaaaattt ttatacctat
     4861 tcttatctgt ctttatggtt tttttccttt tttcctgttc ctcacaatcc gcattccaga
     4921 acactcagaa tgctttgccg ggaactcctg aaaaaattcg ttttactgtt tcttttattg
     4981 tcacggtacc tcagaacaca gattatgttt atataatggg cgactttaac aactggaatc
     5041 cgggtgatga agagtacagg ctgaaaaaaa tagattctgt tacttattca ataacgcttt
     5101 cacaggaaaa ggggaaaacc ataaacttta aattttccaa aggaacttta ttttcagttg
     5161 aaacagattc gaatttttac gaaaccgatc ccaggacttt cagatttgat tataacaatg
     5221 atgaggtaag atttgaagtt gaaaattggc ttgacaacaa gtaaacccga tacataagcc
     5281 agaagaaatc tggctttttt ggactcatat attatttata atcttataaa tacttataat
     5341 ggagggtaat atgaaaaaag ttgctgtcat taccaccggc ggtactattg ctatggcaca
     5401 tgatccaaac atgggggtta tcctttcaga aaacaaaaac aaagaactca gtgaaattcc
     5461 tcaaatacat cagattgcag atatcgaatt ccatgagttt ttcgatgtgc ccagtccata
     5521 tattacccca ggcatgatgt ttgagctctc aaaatacgtt aaaaagatcc tttccagatc
     5581 agatatagat ggagtggtca tcacccatgg aaccgatact cttgaagaaa cggcttactt
     5641 ccttgaccta accattggat cagaaaagcc tgtcgtagta acagccgcaa tgagaaactg
     5701 gagtgagccc agtacagacg gtcctatgaa tcttattgca tctgtaaaaa tagcttcatg
     5761 tgacctatcc gtcgaccaag gtgtcatggt ctgtctcaac gatgaaatac acgccgcgag
     5821 agaagtcacc aaaacataca ctagcaacgt cgcaaccttt gattctccgg gttatggacc
     5881 gttaggcata gtagatgaag atacagttgt tttctttagg cgctcgcttg agagaatgta
     5941 tattcaaacc gacaggatag aggaccgtgt tgcactcatc aaaacatata caggcgataa
     6001 tggagcaata ataaagactc tccctgatat gggatacaaa ggagtcgtta ttgaaggttt
     6061 cggcagagga aacgtacctc cggcagtagc ggatgccatg gagtatcttt tggaaaaaaa
     6121 tataccggta gtagttgtct ccagatgctt caagggaagg gtacttggag tatacggtta
     6181 tataggcggc ggaaaagatt tgaaggaaag gggaataatt cttggtcagg aagtttctgg
     6241 tcagaaagcc cggataaaga tgatcgttgt tttgggaaag acaaaggata cggaaagttt
     6301 acgaagatac tttgaatttc aaaaccgata aggaggatta tcttgattaa aaaccagaaa
     6361 gcaggtcttt ttacaggtat ttatattttg ctttttttta gcattgctat tatttcgatg
     6421 aagatattcg ctattcttgt cttgtctctt ggaagtatac ttgtaataga tctttttgcg
     6481 aaagtcctgt ctaaatggtt taaattccgc agggtggtat cgcttacact atctatgata
     6541 ttcatatttt ccatatttgt tattgctttt gtctttctta ttccggcagt aataaaagag
     6601 attgatactt ttgtagtttt tatagacaga ttttttgtca atagagagtg ggaaaagttt
     6661 tttacaaaca ttttcccgga tactgaaaaa accctttcaa atttcatggt ttcacttcaa
     6721 ccgaaacttt tggaatttct caactccttt gtcggtaagc ttccggcata tgggcagcag
     6781 atcgccttat ttctggcata ctacttcctg ctaactttat atggtattta taatattgat
     6841 tctcttaaaa aatgtattgg ttacctttac ccacgctcaa tacgctctac aggcgagaag
     6901 ttcttttctg aactctacca tagccttgac agtttcgttt tttcaacatt tttttctgca
     6961 atttttgtcg gagtatgtgc ttttttcatt atgagttttc ttggtataaa gtacacagtt
     7021 ttgatgagct tctgggcttt tgtaacaaat tttataccca tagtaggagt aatttttgaa
     7081 tttgtaccgc ttgctcttgt aggtctttca tctggagtag agagcatggt atggcttttg
     7141 gttcttatgt ccgttattca tggatcggca ttcatatttt tcttgtttgt gatgagaggc
     7201 tactcaaaaa taaatccttt ttttattatt cttgccatac taattttcgg tcagatattt
     7261 aatgtcttag gcatactaat agctgttcct ctgtgtctta ttataaaact tttctggacc
     7321 aactttataa aaccctactt tgagaggggc tgattcattt ttgaacttcc gcaaaacagt
     7381 gatttttttg ttatgttttt tatttttttc tatgtctttt ggacttacgg tagaggagat
     7441 ctataatctt tccaaactgc ctcaaaccct ggaaaaatca atagacgagt ttcttaaata
     7501 tttttatgaa aaccctgctg atcctcagct ggaagtttac ggttctctca tatactcaaa
     7561 aaagactata agagacaagt acccatcata tgcttttgta aacggattaa tatcagaaaa
     7621 ttctaatgat tttctgatga gtctaagcgc tatgagtcct tcatttaaga tacctccaga
     7681 agacacccag ttggtagtga tgattttgaa agatcttatt cctaagcttc aaaagatctt
     7741 tgaaacaggt actctacagg aagcttccct caaacattcg tatcacctgg atggaataaa
     7801 tcttatggta actcccaaga cgtataaacc ctttctggag tttgtagttt ttaaaactct
     7861 taacactcct tcttttcttg atgatgatat gaaaaaattt atagagttct ttgttcccag
     7921 aaacattctt tctaccataa acgcgataat ttcatcatcc agattagtac ttaatgaaag
     7981 tgattatatc ggaggttatg tgctccttga ttatataaat acgcttggaa tgattgattc
     8041 tacaaatata gattcttttg taacccttaa aaaatactat acagtgcgta actctattac
     8101 aaaactttca aactctgttt tcatggtgga tgatcaggaa ctgcaggttt atctggatga
     8161 tctttatact cagattgaag agctttcaac actttcctcg tcaaaaaaaa gcatagagga
     8221 gattcttctg ctggtcttaa aaactcttaa tacaagactt gataaaaata ccaaaaagtt
     8281 attttcgaaa agatacgatt ttgacaaagt tcttacttca ttccctgaca atatccaaaa
     8341 tgagctctta cgccttaaat tgaaaattga tgaattttat atatcagcgc aagtaaaacc
     8401 cgatcaaaca aaagtatcaa caggagcaac aagcagttct ggaactgaaa tgaaaactta
     8461 tacatttttt tattttttgg gcactgttat atttttagtt actgcttttc tttttttaac
     8521 catggagttt tttcctaacg ttcagaaaat agagttttta tgtaaaatag gcatgggaag
     8581 atatgccata catttggcag agaagttagt tataaaaaat gcaaatgatt tcagatcttt
     8641 ctttgctctg gccaaagcac ttgaagtatg tggaagatat gaagattcaa ttaatgctta
     8701 taaaacagcc atgagtatac gcgaaaaaaa tcatgaaaat aaaggtgaat agaaatgaat
     8761 tctcttgtat tgagcacctc aatgaaaaaa gtttatattc ttctcataaa aggaaagttt
     8821 tatgatttta ataccatcga ttcaaaaaat tctggaagta agcttgctta tgaagtagat
     8881 aatattctta agaaaaacgc tatctctgtt tctgatatgg atacttttgg aattgataca
     8941 ggtcccggtt cttttacagg tatacgtata ggaatatctt ttctttccgg actactggca
     9001 ataaaaaacg atatggaaat aaaaaacatt atttccactg atattattaa tgaaagcaac
     9061 acatctacaa tctctaattt tgcagttttg cgaagggcgc gcgaagatgc agcatatgta
     9121 tgtatctaca gagatagagt tcttgcaggg caacccaata tgattaaagg ctcagaactg
     9181 gcttatgtct taaaaggatt agaacttaca ggtgaggagt ccagatactt catagataag
     9241 ttctcacttg gtaatacttt ttttgaaaca gaggtcactc ctcaaggcat gatgaaggct
     9301 ttcatgaatt caaaaccagt taaactaaac gatctaaaac ccctttacct tcaaaaaccc
     9361 cttgctgtgg aaatatggga agaaaaaaat aaaattctaa tgaacaaaga gttttggaat
     9421 taaatcgtta aaaaaaacag ccttaaggct gtttttattt ttcgaaaaga aaccctaaag
     9481 caaacgtaag tgatttcaga gaaactacaa aatctacatt ttcaacggaa atattaagag
     9541 gaaccgttat ggtagcaggt gtgaaattga gtataccttt tattcctttt tttattacaa
     9601 gctctgaaac attttgagcc gcctgggcag gaactgtaag gatagcaatc tctataagat
     9661 tatctcttat tatttcctca agatcttcca tcttatatat cttaacatta gtcgaaatgt
     9721 actttcctat cttcttctcg tcattgtcaa acgcagctac aacacgaaaa ccattctttt
     9781 caagaccgga ataattggca attgcattcc caagatttcc tgcacctatt atagcaacat
     9841 tccaactttt attaagaccc agtatttcac gaagtttaaa agaaagctta tccacatcat
     9901 aacctactcc ccttttccca aactccccga agtaagacag atctttcctc acctgtgaag
     9961 cctttatacc aagtatgtag gcaatttctt gtgaggatgt tttttcaacg ttatcctctt
    10021 tgagtttggc caggcaacgg tagtatatcg ccagtctctt taccgttggc ttaggaattt
    10081 tgagcactat taaaacctcc tgcattaaaa caatagtgta ttaattcaca caattattat
    10141 actctgaaaa aaaaaaaaaa gcaacttata aacagcttac tttcccgtaa cagttggaat
    10201 ataaagatta agatctttgt aaagattagc aatacccgtc tggggatatg ctttgagcct
    10261 ggtctgccca ggttcgttta taagaaaaaa aacctttgtg ggatcacccg cattgaaaga
    10321 agcatcatat ggaacatcaa tagtatattt tgtctgaatt tctgcctgtt taaaaaagaa
    10381 aaacttatat acattcaatc ccggttgtaa attactttga aaaacatagt tcccagcaga
    10441 gttcttaaag taacaccata cgtcttttga accagttata tatatctgca tatatccact
    10501 tttgtcactt ttctgcatag acgtaataat atactccaaa tcatccatct gttttttaag
    10561 aaaactattt gctacaatat atttttctac cataccgtta ggtttgaagt atgtatcaac
    10621 cttttccata ttgttttcta tatttgtaac tcttgtgttt atagtaccac tcaatctgtt
    10681 tatatcgctt tgaaactgtt ttttaagcat taccattctt atttccgttc caattgaaag
    10741 aataagagcc gatacagcca aaactataag aataattcta agtttcatat cttccctcca
    10801 agaaaatcat ctatgcgttc tatcaactca gttttttttg acaacaggcc ttttatcttc
    10861 tgtattgtat tatcaacttt tttgaaggca atagagtgct tttcagcaat ctctttgtac
    10921 gaaaaaccat caaaccaatc gtaaattatt accctttcat aatcctgcat caacgatacc
    10981 tcctgttcaa taagataccg tataagtatg aatttggaca tatcctgttc gcatggctgt
    11041 tcaaaaaagc tttcagcatc atcattactg tcttcaaaag tattttcaat gcttaatgcc
    11101 tctgaaagaa ccttgttttt ttgtctgtta agatatgtta taaaaga
//
LOCUS       DAOSHR010000055        11120 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_4945_length_11120_cov_5.745504, whole genome shotgun sequence.
ACCESSION   DAOSHR010000055 DAOSHR010000000
VERSION     DAOSHR010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 11120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11120
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4945_length_11120_cov_5.745504"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1283)
                     /locus_tag="PLU28_04425"
     CDS             complement(<1..1283)
                     /locus_tag="PLU28_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34344.1"
                     /translation="MKKLLLSIIFICTISLLFALDFKTEFSKEIFAGQDFNISIYEDS
                     LTFTDGFIYFRKAGDPQFKSIDMSIFRKNATGFIPYSILQSSTYEFYIQLQDTSANMY
                     RLPQKLGQLYTFEVKNDTVPPLIKLLSPLKGQVFYNDQSIDFVMFIQEDQSFIDSDSI
                     EFLLNGAKITGYNYENGFLTYKIDQPQLGTYKVSLKVMDIYSNKAELKEFELKVENRP
                     APIFDMLATLNYSLNVGSKYEAKKSGHGITFEELLPENPNSDVTITPFSFEQKINLQA
                     ILKMWIFKLGLKVEMNQTNADIQKLFTDFANQAIYDFYDFNRILNPLNFENFNTSSSA
                     IRKIGQSNFYSLYADFGILKYAFGDTKLNFSSNTVTNLNARGHYAKIDLYLASLEAMI
                     GNIDPGVYSASYPRFMAGVNAGINVFDFFKLNADLS"
     gene            complement(1294..8058)
                     /locus_tag="PLU28_04430"
     CDS             complement(1294..8058)
                     /locus_tag="PLU28_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34345.1"
                     /translation="MGIKAKKSVLMFLLFVSFVFTGFGVVKNFSIRSVGPSGLTIDTR
                     YYAILTPGILINDELIVATLQNDSTVTKISADITLKILSGDWNPSGSDDVYIKLRLKD
                     IEIKANETKVLSNKNIISDYLGDMAVLENKLPPELKNITSVEQALTSLELKEGRYKIS
                     MAVFEHSGSLLNKLGENDLTVNVVTPTSMYFIKKADGSSITPTLSWKLSMVPIYASLP
                     ITSNPVYCYSKLMVSSTDGTYSKEIKINHSKPSSGDLKGYPSGDGIADGVYTYTVTGT
                     SGGIILRRGKTYKFKVELYDWYDKKISETSEETVEFDTSEVVAKSPVSDIKILSPKFE
                     WSYKYNSEVGYYKLIIDGFKTYSSISGLSFQMSDQLSWGRSYRWKVVPFYSDGTVFIE
                     EAATPWNNFSTIVNSKPVTEIISPSEGSLLIKGRSYSFKATASEPDKAEGVDDEITAT
                     WWEIGSRKYTGNDLSFTPETRTGSSPLKITYKAQDKYNNIGQSQVNVFIIDPYINITR
                     PKNSSTGFVGDEIAFECISQDVQEITWYANSKPISPKHTFSTAGSYEITAKATVQGIE
                     IKSPSITINIIEKPSLQAITDPELTTYQSRGVTLKVGFKNLDPAKISWKINASVSDKK
                     GDSFNFVSDTSGTFIIKAFFETAEVVFTINVKKIPSVKIVSPVDTSVINAQEDILFKA
                     QASDYTELVWYIDSKEFARGASVSFNFDPTYEGEHLIQLRADDLDMPAANDSVRIKIN
                     VVKELVITEPVQDPFVTDVNSEITFTAKVKNLPEPSWYVNTEKKGTGSSFKYLFSLTG
                     EYKITVSSNSYTDAVTVKVVGQKYLTIASPKNGSVIEIDKVITFTSDSKNIRSDIVWY
                     INDEEVVKGSSMSYTFRTPGTYKVKASGDGYSDEITLTAVPPEKLSIIYPLNNQQVYI
                     NKDILFQSESSDLRSSIIWSVNGSMIGQGESISYKFVNPGNYSITAKTSTKESTVQIT
                     AIEPQSINILSPADSLVKLSSKVKLTAKSKYPDEIKWFVDSVLIGTGDSLEYIFDKPG
                     KLLIKAQTADAQAEKEITVYTPVSDLLITSERYVYYSDELATFSCSFNLDPYLGLRNI
                     QWLIDGNKISDKNTLNLDLTSISAGLHSLVVTVTDSQSNSISGSITFDVVSHLEYEIT
                     SPKDNKNINSSEVLRLNAKLTKGLSKDIKNIEWFVNSKSVGSGWEVLASNPGKGTTEI
                     VLVIKDRLEKEYKKSITVNISDKLEVKILKPSNFSLFKYGQEIKATAQAYLYTEKGMQ
                     LLDSKDIKWFIDEISSNGSGILFINNLSIGSHKLKAVYDQSYDEVAIEVSQPRSIVIT
                     EPAENTVINSGSKLLCRAQADESATINWYIGSKKVASGKEVYIDTNQIMGKFVIKAES
                     QYLDFFSEASVNVSINEKPVITWISPKDGDVFASGAKINIKAEANDYEDGKIPYTLFV
                     DNSEIQGTALLNAADLGSGEHILTIAVNDSNKLKTISTIKIRINEPPKISLVSPLENQ
                     EFTFLSPINLSCSVSDDEPLKEENILWFMDNTQIAKGSNVSYTQELAQGVHKITVRYS
                     DSLNEQISKEINFKVLAKPSVKIYTPVNGQIVNLNEKITLKAWAFKADGKKYAPSEML
                     WFIGSTPAGQGEQIEFTALSKEPVIITCRTTEASDNITLNVNTPPVVRISSHPDGYSI
                     MPSEVFIVKADVYDLQEDTDPTKISWQVDGKTSGKGISVNLSDLEQGEHLITVSVTDS
                     MGLMASASIKVSKTAVLKAQAKLESDSLYYLFSEKIKGSISFSGGVAPVTASWTVKQP
                     GKKDTVITNTELDVSGQLLSEGPAILNARISDSKGSIVEYNFEITIIDNLKVQITGIE
                     DGSTFVKPTENIQASLKVYNPKNLAVSYTWTINGQSVSSLKDLSLETKNYNEGSYFLK
                     AVAVSSDGTIAEDGLTIFIRPKLKVEIRSKITDHTENSDLQLVATAFDPVEGELENSS
                     ISWISNLQGELGKGKNITVKLQKGKHIITAVAVNKSGQKAYDYLTVLVLGNPEINIVS
                     PAENSVFNEGEQVPLKSIASDSDMEPINEKLIAWSSNIDGAIGFGSSLSTSSLSVGQH
                     IITATCTGKYGGLKTASIQIEVMKARNISKGLKIAKDKNSLVLSDTSFKLECYRIGDI
                     TGDVVWFSDIEGKIGEGDSTSALLKTPGTHTITANLNEYSDSITILVTEYKPRLVPYG
                     IVLDLKGDVKIKNSTNNSFVPLIPLTVLYENDELSLISGSFVKIMKANGSSELINGPA
                     TKTLGK"
     gene            complement(8170..8526)
                     /gene="rplT"
                     /locus_tag="PLU28_04435"
     CDS             complement(8170..8526)
                     /gene="rplT"
                     /locus_tag="PLU28_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOG34346.1"
                     /translation="MRVKRAVSARKRKKRYLKRVKGFRGAVSRRISLARDKFFKAGVY
                     AYTGRKDKKGDFRRLWITRINAAARLEGMKYNELIHGLKLANININRKMLAEIAVNDF
                     DSFKEYCSIAKKALTK"
     gene            complement(8557..8760)
                     /gene="rpmI"
                     /locus_tag="PLU28_04440"
     CDS             complement(8557..8760)
                     /gene="rpmI"
                     /locus_tag="PLU28_04440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOG34347.1"
                     /translation="MAKIKNKTKSAAKKRFKITATGKILRKRSHIGHNSGTKTNTHMR
                     RLTRITEVSEVQKKNVERCLGLR"
     gene            complement(8773..9315)
                     /gene="infC"
                     /locus_tag="PLU28_04445"
     CDS             complement(8773..9315)
                     /gene="infC"
                     /locus_tag="PLU28_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOG34348.1"
                     /translation="MSEKDIILKNEQIRSQEVVLITQSGENIGSVLTKTALEMAREAE
                     LDLVLVSPNSNPPVARIMNFSKFKYERERKEKEAKKNQKKQELKEMKFRLRIDEHDFQ
                     TKVNRIREFLSDNCKVRVVVMFLGRDIMFKDKGEELLKKVIAQTSDISNVTKNIKMNG
                     RDMDIFLDPDLKKAVPNQTK"
     gene            complement(9456..9531)
                     /locus_tag="PLU28_04450"
     tRNA            complement(9456..9531)
                     /locus_tag="PLU28_04450"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9496..9498),aa:Lys,seq:ctt)
     gene            complement(9613..9804)
                     /locus_tag="PLU28_04455"
     CDS             complement(9613..9804)
                     /locus_tag="PLU28_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOG34349.1"
                     /translation="MYRYCCPVCGNEFSVMHSMNDKPVVKCDKCGNTAKKSISSVGIS
                     FKGSGYYITDSKTTSKHST"
     gene            complement(9916..10542)
                     /locus_tag="PLU28_04460"
     CDS             complement(9916..10542)
                     /locus_tag="PLU28_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosylase/DNA lyase"
                     /protein_id="HOG34350.1"
                     /translation="MNLKQTLYGLENKITDLVETRFHEFETLGRHGSNEELFSELCFC
                     VMTANWTAKGGMKIQSIVGPDGFAYMSEDELTRTLRQNGHRFWRTRAKYICANRWFIK
                     DVKSILDKPAFECRKYLAENLMGLGWKEASHFLRNTGAKDIAILDKHILKLMMEDGLI
                     DQLPKNGWTEKKYLSHEGILRNFSEGVGHSLGKLDLYLWYIAKGSVDK"
     gene            complement(10552..>11120)
                     /locus_tag="PLU28_04465"
     CDS             complement(10552..>11120)
                     /locus_tag="PLU28_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34351.1"
                     /translation="IIFLLGFYSAYTMLIPYIMSFSQVKDFMSQFSEYNSIIFLAFAA
                     LMGIMFYFIIKVAVGIVGFLLGGFIGYFLGNSLIVIFSEQLKQFESISYYIPWGCLII
                     IGIITAVLISKNFETITAIISIVSGSLLMSFYSLFLLEKHTGMKIGGNTLLKGLDNVS
                     KSEGLMLLFCFIIYCSLGFFIISRKKKA"
BASE COUNT         3102 a         2147 c         2056 g         3815 t
ORIGIN      
        1 gataaatcag cattaagttt gaaaaaatca aaaacattta tacccgcatt aactccggcc
       61 ataaagcgtg ggtaggaagc tgaataaaca ccagggtcta tattcccgat cattgcttcc
      121 agactggcaa gataaaggtc tatttttgca tagtggcccc ttgcattaag attggttacc
      181 gtgttggaac taaagttcag cttggtgtcg ccaaaagcat acttgagaat tccaaagtct
      241 gcataaagac tatagaagtt gctttgtcct attttcctta tggcagagga ggaggtgtta
      301 aagttttcaa aatttaaagg gttcagtatc ctattgaaat cataaaaatc atatatagcc
      361 tggtttgcaa aatctgtaaa gagcttttga atatctgcgt tggtctgatt catttcaact
      421 ttcagtccaa gtttaaatat ccacatcttc aagattgcct gcagatttat tttctgttca
      481 aatgaaaaag gagttattgt tacatcagag tttggatttt caggtagtag ctcttcaaag
      541 gtaattccgt gtccggactt ctttgcttca tattttgagc ctacatttaa tgaataattt
      601 agagttgcaa gcatatcaaa tataggtgcc ggtctattct ctactttcaa ttcgaactct
      661 ttcagctcgg ctttgtttga gtatatatcc attactttaa gtgatacctt gtatgttcct
      721 aactggggct ggtctatttt gtaagtcaga aacccgtttt cgtaattata gcctgtaatt
      781 tttgctccgt ttaggagaaa ctctatagaa tcactgtcta taaaagactg atcttcctgt
      841 ataaacatta caaaatcaat ggactggtca ttgtaaaata cctggccttt gagtggagag
      901 agcagtttaa tcagtggtgg aacagtgtca ttttttacct caaaggtgta cagctgcccg
      961 agtttttggg gcagcctgta catattagca ctggtatcct gaagctgaat atagaactcg
     1021 taagttgagc tctgcaaaat agaataaggt atgaagcctg tagcgttttt tctgaaaatt
     1081 gacatatcta tactcttaaa ctgaggatca cctgcttttc tgaagtaaat aaaaccatct
     1141 gtaaaagtga gactgtcttc gtatattgag atattaaaat cctgtcctgc gaatatttcc
     1201 ttactgaatt cggttttaaa atctaaagca aataataaag agatagtgca gataaaaatt
     1261 attgaaagta aaagtttttt cataagtcat ctcctacttg cctaaagttt ttgtagcagg
     1321 accgttgatg agttcgctgg agccgtttgc tttcattatt tttacaaaag aacctgatat
     1381 caaagatagt tcatcatttt cataaaggac agtcagaggt ataagcggaa caaaactgtt
     1441 attggtagag tttttgatct taacgtctcc ttttagatca aggacaattc catatggaac
     1501 cagtctaggc ttatattctg tgaccaggat agtgatgctg tcgctgtact catttaaatt
     1561 agctgttatt gtatgtgttc cgggagtttt aagtaaggct gaagtactgt cgccttcgcc
     1621 gattttacct tctatatctg agaaccagac cacatcgcct gttatatcgc ctatacggta
     1681 gcattcgagt ttgaagcttg tgtcagaaag tactagagag ttcttatctt tggctatttt
     1741 cagtccttta gatatgtttc gggctttcat tacttctatc tgaattgaag ccgtcttgag
     1801 accgccatat ttaccggtac aggtggcagt tattatatgt tgtcctacgc tcagagaact
     1861 tgtgctgagt gaggagccga atcctattgc tccgtctata ttggaagacc atgcaatgag
     1921 cttttcattt ataggttcca tgtcactatc gcttgcgatt gatttcaaag gaacttgttc
     1981 accttcattg aagacagagt tctccgcagg agatacaata tttatttcag gattgcccaa
     2041 aaccaggact gtcaagtagt cataggcctt ttgacctgat ttatttacgg ctactgctgt
     2101 tattatatgt tttccttttt gaagttttac ggttatattt tttccttttc caagctctcc
     2161 ctgcaggttg gatatccagg aaatcgagga gttttccagt tcaccttcca caggatcaaa
     2221 agctgttgcc acgagctgta agtcagagtt ttcagtatgg tcagtaattt ttgatctgat
     2281 ttccacttta agttttggac ggatgaatat cgtaagtcca tcttctgcaa ttgtaccgtc
     2341 cgatgaaact gctacagctt taaggaagta ggaaccctcg ttgtaatttt tggtttcgag
     2401 tgaaagatct ttaaggcttg atacggactg tccgtttatt gtccacgtat acgatactgc
     2461 caagttcttt ggattataga ctttaagaga tgcctgtatg ttttcggtag gcttgacgaa
     2521 agtactccca tcttctatac cggttatctg tacttttaga ttgtctatta tagtaatttc
     2581 aaagttgtac tcaactatac tgccctttga atctgatata cgggcattta gtatggcagg
     2641 tccttctgaa agcagctgac cgcttacatc aagttctgtg tttgtaataa ctgtatcttt
     2701 tttcccaggc tgcttaactg tccatgatgc tgttacagga gctactccgc cgctgaagct
     2761 tatactgcct tttatcttct cgctgaaaag ataataaagg gagtcacttt caagctttgc
     2821 ctgagctttc agtacagccg tcttgctgac ttttatggaa gcagaagcca tgagtcccat
     2881 agaatcggta accgatactg ttattaaatg ttctccctgt tcaaggtcgc tcagatttac
     2941 ggagatacct tttcctgaag ttttaccgtc aacttgccat gaaatcttag taggatcggt
     3001 atcttcctgc aagtcatata catcagcctt cactataaaa acttccgaag gcattataga
     3061 gtatccgtca ggatgtgagg atatacgtac cacaggaggg gtatttacat taagagttat
     3121 gttgtccgaa gcctctgtgg ttctgcaggt tataataaca ggttctttag ataaggctgt
     3181 aaactctatc tgttctccct ggcctgcagg agtactgccg ataaaccaga gcatttcgga
     3241 aggagcgtac tttttaccgt cagctttaaa tgcccatgct ttaagggtaa ttttttcatt
     3301 taaattaaca atctggccat taactggagt ataaattttt acagatggct tagcaagcac
     3361 cttaaaatta atctctttgc ttatctgttc gttcaaggag tcgctgtatc ttacagttat
     3421 tttgtgaact ccctgcgcta actcctgtgt ataacttaca tttgagcctt tggcaatctg
     3481 agtgttgtcc ataaaccaga ggatgttctc ctcttttaaa ggttcatcat cagatacgga
     3541 gcatgagaga tttataggtg aaaggaacgt gaactcttga ttttccagag gacttacaag
     3601 agatatcttc ggaggttcgt ttatccttat ttttatggta gagatggtct ttaatttatt
     3661 tgagtcgttt acagcgattg taagtatatg ctcgccgctt cccaaatctg cagcattaag
     3721 aagtgctgta ccctgaatct ctgaattatc aacaaagaga gtataaggta ttttaccgtc
     3781 ttcataatcg ttagcttcag cttttatatt tatttttgct ccagaagcga aaacatctcc
     3841 gtcttttggt gatatccagg ttattactgg tttttcgttt atacttacat ttactgaagc
     3901 ttctgaaaaa aagtcaagat attgcgattc ggcttttatt acgaactttc ccatgatttg
     3961 attagtatca atgtacactt ctttgccgct ggcaactttt tttgaaccta tataccagtt
     4021 aattgttgcg ctttcatcgg cttgcgccct gcaaagaagt tttgaaccgg agtttattac
     4081 ggtattttca gcaggttcag ttatgactat gcttcgtggt tgtgaaactt ctatggccac
     4141 ttcgtcataa gattggtcat ataccgcctt cagtttgtga ctgcctatag acaggttatt
     4201 gatgaagaga attcctgaac cgttggaaga tatttcatca ataaaccatt ttatatcttt
     4261 cgaatcaaga agctgcatgc ccttttcagt gtacagataa gcctgcgccg tagccttaat
     4321 ttcctgaccg tacttgaata agctgaagtt agagggttta agtattttta cttcaagttt
     4381 gtcagaaata tttacagtga tagatttttt gtactctttt tccaggcggt cctttattac
     4441 aagaactatt tctgtagtac ctttcccagg atttgatgca agaacttccc atcctgagcc
     4501 aacagatttg ctgtttacaa accattcaat gtttttaata tcctttgaca atccttttgt
     4561 gagcttggca ttgagtctta aaacttctga actgttaata tttttattat ccttgggcga
     4621 agtgatctca tattccagat gagacaccac atcaaaggta atagaaccgg aaatagagtt
     4681 ggattgactg tctgttaccg taactaccag actgtgcagt ccggcagata tcgatgtaag
     4741 atcaagattc agagtatttt tgtcagatat tttatttccg tcgataagcc attgaatgtt
     4801 tcttaatcct aaatatggat ccagattgaa tgaacatgaa aatgtagcta gttcgtcaga
     4861 atagtaaacg tatctttcgg atgtaataag cagatcagat accggcgtat aaaccgttat
     4921 ttctttttct gcctgagcat ctgctgtctg tgcttttata agtaattttc ctggtttatc
     4981 aaaaatatat tccaaagaat ctcctgttcc gataagaaca gagtctacaa accactttat
     5041 ctcatcagga tactttgatt tggctgttaa cttcactttg cttgaaagtt ttaccaaact
     5101 gtcagcaggt gagagtatgt tgatactctg cggctctata gctgtaatct gaaccgttga
     5161 ttcttttgtt gaagtttttg ctgttatact gtaatttcca gggtttacaa acttgtaaga
     5221 aatactttcg ccctgaccga tcatagaacc gtttactgac caaattattg aacttctcag
     5281 atctgatgat tcgctttgga aaagtatatc tttgtttata taaacctgtt gattgttaag
     5341 aggatagatg attgacagtt tctctggcgg cacagcggta agagtgattt catcggaata
     5401 gccatctccg cttgccttta ccttatatgt tccgggtgtt cggaaagtat agctcattga
     5461 agaacctttt acaacttcct catcatttat ataccataca atatcagacc ggatattttt
     5521 ggaatcagaa gtaaaggtaa ttactttatc aatctctata acgctaccgt ttttcggaga
     5581 agctattgta agatactttt gacctacaac ctttaccgtt acggcatcgg tgtaagagtt
     5641 tgagcttacc gttatcttat actctcctgt caaggaaaag agatatttaa aagaacttcc
     5701 tgttcctttt ttctcagtgt tgacgtacca acttggttca ggaagatttt tgactttagc
     5761 agtgaaggtt atctcgctgt ttacatcggt tacaaatgga tcctgaacag gttctgttat
     5821 tacaagctct tttactacat ttatctttat gcgtacagaa tcgtttgcag caggcatatc
     5881 aagatcatct gcacgaagtt gtatgagatg ctcgccttca tatgtggggt caaagttaaa
     5941 gcttacggaa gcacctcttg caaactcttt agaatcaata taccatacca gctccgtata
     6001 atcactggcc tgagctttga acagtatgtc ctcctgtgcg ttaataacac ttgtgtcaac
     6061 gggtgaaacg atttttacag acgggatctt ttttacattg attgtaaaaa ctacctctgc
     6121 tgtttcaaaa aatgctttta taatgaatgt gccagaggta tctgaaacga agttgaagga
     6181 atcacccttt ttgtctgaca cagaagcatt gatcttccat gagatttttg ccggatcaag
     6241 atttttaaaa ccaactttga gagttacacc tcttgattgg taggttgtta attctggatc
     6301 ggtaatagcc tgaagcgaag gtttttctat aatatttata gttatggacg gactctttat
     6361 ttctataccc tgtacagttg ccttggcagt tatttcatag cttcctgcag tactgaaagt
     6421 atgctttggg gatataggtt ttgaatttgc ataccaggtt atttcctgaa catcctgtga
     6481 tatgcattca aaggctattt catctcctac gaaaccggtt gaggagttct tgggtctggt
     6541 aatgtttata taagggtcaa taataaagac gtttacttgg gactgaccaa tattgttata
     6601 cttgtcctgt gctttatagg ttattttaag gggagaagaa ccggttctcg tttcaggtgt
     6661 aaaagacaga tcgtttcctg tatactttct agatcctatt tcccaccatg tcgcagtaat
     6721 ttcatcgtca actccttcag ctttatctgg ctctgaagca gtagctttga aagagtagga
     6781 tcttcctttt ataagcaaag aaccttcaga tggagatata atttcagtta caggtttact
     6841 gttaacaatt gtggaaaagt tattccaagg ggtagctgct tcttctataa aaacagtacc
     6901 atcgctgtaa aacggtacaa ctttccatct ataggatctt ccccaagaca actgatcact
     6961 catctgaaaa gaaagtccag agatggaaga ataggtttta aaaccgtcaa ttatgagttt
     7021 gtagtatcct acctccgaat tatacttata gctccattca aacttcggag aaaggatctt
     7081 tatatctgaa acaggagact ttgcaacaac ttctgaagta tcgaactcta ctgtttcttc
     7141 agatgtttca gatatttttt tatcgtacca gtcgtacagc tctaccttga acttgtatgt
     7201 cttaccacgg cgcagtatta taccgccgct tgtaccggta acagtataag tataaactcc
     7261 atctgctata ccatcaccgg aaggatagcc cttaagatct ccggaggaag gttttgaatg
     7321 gtttatcttt atttctttag aataagttcc gtcggtactt gaaaccataa gctttgagta
     7381 acagtatacg ggattgctcg ttatcggaag agaagcatat atagggacca ttgaaagttt
     7441 ccatgataga gtgggagtaa tggaagagcc gtctgccttt tttatgaagt acatggaagt
     7501 aggagttacc acattaactg tcagatcgtt ttctccgagt ttatttagta gtgaacctga
     7561 atgctcgaaa acggccatag aaattttata ccgtccctct ttaagttcca gacttgtgag
     7621 ggcctgttca acagaggtta tgtttttcaa ctctggagga agtttattct ccaatactgc
     7681 catatccccg aggtaatcac taataatgtt tttattgctt aatactttgg tttcgttggc
     7741 ttttatctca atatccttaa gcctaagctt tatataaaca tcatcactgc ctgaaggatt
     7801 ccagtcacct gacaagattt taagagtgat atcggcgctg attttggtaa ctgtggaatc
     7861 gttttgcaaa gttgcaacta tcagctcatc gtttataagt atcccaggtg tcagtatcgc
     7921 atagtaccgg gtgtcgatag tcaggcctga cggaccgaca ctccgtatgg aaaagttttt
     7981 gactacccca aaaccagtaa aaacaaaaga aacgaaaagc agaaacatga gaacactttt
     8041 ttttgctttg atacccatag tatcagctcc tcttaaaaga ttaataacaa taatacagat
     8101 attattatac cacaaaaaca ggtatatcaa aaaaaaatta cccggataaa atccgggtaa
     8161 tttataaatt tacttagtaa gtgctttttt cgctatggaa cagtattcct tgaaagaatc
     8221 gaaatcattt acggcaatct cagcaagcat ctttctattt atattgatgt ttgcgagctt
     8281 aagaccgtga ataagttcat tatacttcat tccttcgagt cttgcagctg cgtttattct
     8341 ggtaatccaa agtcttctga aatcaccttt tttgtctttt cttccggtat atgcataaac
     8401 accggcctta aagaacttat ccctggcaag tgaaattctt ctgctaactg cacccctaaa
     8461 acctttaact cttttaagat atcttttttt cctttttctt gcacttacag ctcttttaac
     8521 tctcatctat tatcgctccc ttcaattaac tttgatttat cttaaaccaa ggcatctttc
     8581 aacatttttt ttctgtactt ctgaaacttc agtgattctg gtaagtcttc tcatatgtgt
     8641 attggttttg gttccggaat tatggccaat atggcttctc tttctgagaa ttttacctgt
     8701 ggctgttatt ttaaacctct tttttgcagc gcttttggtc ttgttcttta tttttgccat
     8761 acttgttcct ccttattttg tttgatttgg tacagctttt ttaagatccg gatcaagaaa
     8821 aatatccata tctcttccgt tcatttttat attcttggtt acgttggaaa tatcgcttgt
     8881 ttgcgcaatt acttttttaa gaagttcttc acccttatcc ttaaacataa tatctcttcc
     8941 caagaacatt actacaaccc gcaccttgca gttgtcggat aaaaactccc gtattctgtt
     9001 aactttagtc tgaaaatcgt gctcatctat tctaagtcta aatttcatct ctttcagttc
     9061 ctgctttttc tggttctttt ttgcttcctt ctcttttctt tctctctcat atttgaactt
     9121 actgaagttc ataattcttg ctacgggagg gttggagttg ggcgaaacca aaaccaggtc
     9181 aagttctgct tcacgtgcca tttctaaagc agtctttgta agcacactac cgatattttc
     9241 accactttga gttatcaata cgacttcctg tgatcgtatc tgttcgttct ttaatataat
     9301 atccttttca cttataagat atcactctcc ttatcaaata tagaaaaacg agggcagaag
     9361 acaacaatct accctcgtaa caattttatt attacctagg tagcttaatg ctttaggtgg
     9421 gaaacaaatt agggtatgtt tccgcttaga tattatggtg ggccatgcgg gattcgaacc
     9481 tgcggcctac tgattaagag tcagtcgctc taccaactga gctaatagcc cactcttgtg
     9541 taccgaaatt tattatacct tataaagcga catttgtcaa tcggaattta tttcctttca
     9601 gtaaaatttc ttttaagttg aatgcttgga tgtcgtcttt gaatctgtta tatagtatcc
     9661 tgagccttta aaagaaattc ctacgctgct tatgcttttt tttgctgtat ttccgcattt
     9721 gtcgcatttt actacaggct tgtcgttcat tgaatgcata acactaaatt cattcccaca
     9781 gacagggcag caatatctgt acattggcac aatatacacc tccgtcatat attttaataa
     9841 gtattttcat catttacagc atagtatgct atcactatta tttttagaag cggttaaaaa
     9901 atagttaagt taatcctact tgtcaacaga gcctttagct atataccata ggtacagatc
     9961 aagctttccg agagaatgcc cgacaccttc ggaaaagttt ctcaggatac cttcatggct
    10021 taagtacttt ttctctgtcc agccgttttt cggaagctga tctattaatc cgtcttccat
    10081 cataagcttt aatatatgct tatccagtat ggcaatatct tttgctccag tatttctgag
    10141 aaagtggctt gcctctttcc agccaagtcc cataagattt tcggcaagat attttctgca
    10201 ttcaaaagca ggcttgtcaa gaatactctt aacatcctta atgaaccatc ggttggcgca
    10261 tatgtatttt gcgcgtgtac gccagaatcg gtgaccgttt tgcctgagtg tgcgggtaag
    10321 ctcatcttcg ctcatgtatg caaatccgtc aggaccaaca atactctgaa ttttcattcc
    10381 gccctttgcc gtccagttgg cagtcataac gcagaagcaa agttctgaaa agagttcttc
    10441 atttgatcca tgccttccta gagtttcaaa ctcatggaat ctggtttcaa caaggtcagt
    10501 tattttattt tcaagaccgt acagagtttg ttttagattc aagatgctct cctaagcctt
    10561 tttttttctg gatattataa aaaaaccaag actacagtat attataaaac agaaaagtaa
    10621 cataagtcct tccgatttgc ttacattatc caagcccttt agcagagtat ttccgccgat
    10681 cttcatgccg gtatgttttt ccaagagaaa tagagagtaa aatgacatca gaagtgatcc
    10741 ggatactatt gatataatag cggttatggt ttcaaaattt tttgaaataa ggacagcggt
    10801 tataattcca attattataa gacagcccca gggaatgtag taggaaatgc tttcaaactg
    10861 cttcagctgt tctgaaaaaa ttacaatcag cgaatttccc agaaaataac caataaatcc
    10921 gcccagaaga aaacctacaa ttcccacggc tactttgatg ataaagtaaa acataattcc
    10981 cataagtgca gcaaatgcaa ggaagataat agagttatac tcggagaact gactcataaa
    11041 atcctttacc tggctgaagc tcattatata aggtatcagc atagtataag cgctgtaaaa
    11101 gccaagaaga aaaattataa
//
LOCUS       DAOSHR010000056        11041 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_4985_length_11041_cov_6.012197, whole genome shotgun sequence.
ACCESSION   DAOSHR010000056 DAOSHR010000000
VERSION     DAOSHR010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 11041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11041
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4985_length_11041_cov_6.012197"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="PLU28_04470"
     CDS             <1..490
                     /locus_tag="PLU28_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOG34352.1"
                     /translation="LPIVEGTTVSTKYGPVKTDFILFIAAGAFHVSKPSELIPEFQGR
                     FPVRVELQPLTEEDFVRILVEPENALTKQYKSLLWTDDVDIKFEESGIKCLANIAFTL
                     NDKIENIGARRLYTVIEKVLEEVSFLAPSGSAWSVSIDENYVKNRLDKVVEDKNLREY
                     IL"
     gene            502..1131
                     /locus_tag="PLU28_04475"
     CDS             502..1131
                     /locus_tag="PLU28_04475"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG34353.1"
                     /translation="MEFYEELNEVIIIEDSDSVINLYIRLLSNFMNRKVMPRVIKTFQ
                     EVNTILSESSKLLRERVSLIIAEAEIEDREVLDQLSMIKKTLPKTKVYIISNKVNIER
                     LKKALKSDLSDWIEKPITPDEFYEIVNMSLFRGQTYDSKARELDNEIKEFDPDNDLKY
                     YTLENMISKLILENPSRFEGHYLLGKLYERRGIKTLAQKHYDAAQALKD"
     gene            1145..1570
                     /locus_tag="PLU28_04480"
     CDS             1145..1570
                     /locus_tag="PLU28_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HOG34354.1"
                     /translation="MKEAVKYIIIIGCGRLGSELALELSLKHSVVVIDKNEESFERLA
                     NRNFTGFTMLVDINDMDVLKKMKLEKADMVYVVTPDDNLNFMLAYGIKKMMPHIKIMA
                     RANDPMKKEIFMREGMEIFCPIENSVSGLVKDFYEDDKQ"
     gene            1567..2244
                     /locus_tag="PLU28_04485"
     CDS             1567..2244
                     /locus_tag="PLU28_04485"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HOG34355.1"
                     /translation="MRKKLFYIFEGQTLAYALAKKLLLVGNEVFYISKDIKNIEVIDG
                     LKAFFPSLELIHHDPTDINWVQSLDMSSNVGAVIVISEDDALNFVITWMIRQLYDDVR
                     VIVLVNHPENEFMFKALRVNTLTPISWMQKLIESFLNYDDITAYFNPYIEKLSILELM
                     ITKNDSACNKMLKDLNMPGNSIIGVIIKQDGSIVVPKGNTVVHENDKLLVLAAKEQVS
                     TVKDILK"
     gene            2256..3806
                     /locus_tag="PLU28_04490"
     CDS             2256..3806
                     /locus_tag="PLU28_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HOG34356.1"
                     /translation="MPDYMFYLKMRYKSVFSALAKVMFMQSILILIVGALAFVFSQGN
                     HFFPFFYTSLLSFLITVIFKVLSRGNKTESMNVQDAMLTVFFIWTISILVSAMPFYFG
                     KILGLTQSIFESASGWSTTGLTVIADVESIPKVYLLWRSIIQYIGGAGFALIMIVVAG
                     NSGAGLYQAEGRTDNLVPNIRDSAKLILVIYLIWAVAGITALMIFGKLSFFDAFNHAL
                     TALATGGFSTKNSSIGYFNNVTAELIIMILMISGATGFGVHYSLVQLIKNYFKSRKDL
                     TNGKINRLDFKERMKAEPFLKNPEPKTMFYTLVISIILLVVLCTVHIYSLPQAVRHSV
                     FTAISALTGTGFQTVTFEKWNTLGLFVITILMIFGGMTDSTSGGLKTFRVYVIIKVMI
                     LQVKNYFRPQGTEHHIEVYKGVGKKKIDLGVFKEVIAVVFMYIIVFVTGSLILVGHGF
                     SLEDSMFEFSSALSAVGLTVGITAPDAPSSILWTLIGGMYLGRFEFFLIFYAVIRLIK
                     DLMLKLKN"
     gene            complement(3827..5125)
                     /locus_tag="PLU28_04495"
     CDS             complement(3827..5125)
                     /locus_tag="PLU28_04495"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG34357.1"
                     /translation="MNFLKKMNMSPTMKYAIAEGATYNAAYIGTQGFIFTTLSIYFNA
                     TPLQLSIMTTMPLAASMIQIIIPYLYRIFRNRKELLVITAFFARIATVLIPISILFDF
                     TNSYILFVVMMIFSIFSSFTGNVWTSAMKQIVPDSVRGSYFGLRNYITSIITVVFTMV
                     YSYLMKTDSTKLGLLIVSTLMFIFFISSVFLLSKHSIPDTLIKKDKIDNPFTPFKDKD
                     FRKYLLFTSFWNFSIEFCKPFFSYYQVAILKADYTFLGLASTISALLSMFLYILYGKF
                     SDKFGNKNTIRIGIIFSLTGPLIFLFMTPDNYIMMTLLDAFITSFAWAAINLGYFNLL
                     LESAKEPSEMYFSLNAFICGISAIAASFIGGNLGNLLQKKSFVIFDHLFFGIQFIFFL
                     EILLRIISILILNRVKTAKGEKKFTLFDILPEINSMLKRN"
     gene            complement(5233..5742)
                     /locus_tag="PLU28_04500"
     CDS             complement(5233..5742)
                     /locus_tag="PLU28_04500"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOG34358.1"
                     /translation="MEKGIIGVLKYALSRELEGRNFYLQKVSDIRNESVKKLFLELSE
                     MEDEHVEFINKLIGNISENSTAIADLSSDDNFFNSRKNSEFSYTTPQGHESFDTSEIA
                     IIRMAYLIEDDFMKFYEEASFYTDNKDLKKILLSLAKWEKTHRDALYGLYRQSMQNYW
                     FDMGFEPLL"
     gene            complement(5911..7134)
                     /locus_tag="PLU28_04505"
     CDS             complement(5911..7134)
                     /locus_tag="PLU28_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HOG34359.1"
                     /translation="MMDVILGRQKIKWVEKHMKIVNFLRDSYKDSQPFKDVNISMAIH
                     LEAKTAYLSIIMKELGANVFITGSNPLTTQPDVVMALKEYGINVFAEKTLDETVYWKN
                     IDRVLSAKPNIIIDDGADLGVRFFENYSSDLNNLWGICEETTTGVKRYNSLQKCGKLP
                     VPVIAVNDSYMKYLFDNRYGTGQSTWDGIMRSTNLTVVGKTVVIGGYGWCGKGIAMRA
                     KGLGANVIVTEVDQIKAIEALMDGFRVMPMDEACLLGDIFVTATGNIDIITEKHFLKM
                     KDGALLANAGHFDVEVKVADLKKISDCITEVRPGVEECVLKNGHSVFLLGKGRLVNLV
                     NADGHPVEIMDQSFALQLEGAKYILNNHSTMPKNVSPIPYDIDIKLASIKLASMGIKI
                     DSLSQDQKKYMQSWK"
     gene            complement(7487..7723)
                     /locus_tag="PLU28_04510"
     CDS             complement(7487..7723)
                     /locus_tag="PLU28_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34360.1"
                     /translation="MKKLFVMLSALLLLASSVCANDISFDRKNYGGNVLLWPFWGIYT
                     GASGYYKMGTDLNITIMDQTSPIVVAGPSFDVGF"
     gene            7913..10039
                     /locus_tag="PLU28_04515"
     CDS             7913..10039
                     /locus_tag="PLU28_04515"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HOG34361.1"
                     /translation="MEQAQEYLREIQSIFKRELTAEEASKITVAYDLAQISHQGQMRA
                     SGEPFFEHPKEVGRILAKFNMDVDTIASGLLHDVCEDCGVPIDVIREKFGNDVARIVD
                     GVTKISNLKLNEKLSKADTKSIEKIETLRKMLLAMSTDLRVIIVKLSDRLHNMRTLAY
                     TSHAQQTSKAQETLKIYAPIAHRLGIHTIKAELEDLSFFYLYPDSYNELKIRVDNKLS
                     DRQKSIEEYSNLLNAELAKHNITAKVYGRSKHIYSIWDKMVRKGKSFDEIYDFIALRI
                     ITENQNQCYASLGIVHSLWPPIPGRIKDYIATPKLNGYKSLHTTVITNKGEPLEIQIR
                     DWQMHQEAEYGLAAHWAYKMGVGHEKIKFLQNLMELHKDIAQSAFDLNEIESELDTAE
                     TFVFTPHGEIIHLPKDATPIDFAYAIHTDVGNHFAGSKINGKIVPISHKLQNGDVVEI
                     IINRNFQGPSMDWLKYARSARTKSKIKKYYRQKNEQLLIEQGKDKFREIAKKLGFSIE
                     DVLLKLKESAFYLKFNIKNDEDLYAKLSLDDVNYLTIKNYVVGTEDKKQSSIKNKSSS
                     SKPTVLIQGMDGVESYYAKCCMPVPGDEIIGVLSRRGIGIHRKNCKIIRDVSDEKVVK
                     VSWNSYDSYIFTATLNIEMKSKEVMNSIRSVITAERASIEMYEMERQLNSLMTKMRVK
                     VNNVEHLTRVIQKISEVNGVSGVRRS"
     gene            10053..10505
                     /gene="dtd"
                     /locus_tag="PLU28_04520"
     CDS             10053..10505
                     /gene="dtd"
                     /locus_tag="PLU28_04520"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOG34362.1"
                     /translation="MRCVVQRVSHAQVDVDNITKGSINKGLMILAAFRDADKREDFEW
                     MADKLLNLRIFEDDQEKMNLSVTDISGELLVISQFTLYGDCRKGRRPSFSESAGLEKA
                     KKDYEEFLQILTSKTNLKVATGVFQAVMKVSLVNEGPVTFIMDSSKVI"
     gene            10544..10738
                     /locus_tag="PLU28_04525"
     CDS             10544..10738
                     /locus_tag="PLU28_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34363.1"
                     /translation="MQETEVTVFIYSQEPFEAHISAEALENGINQTNVLKIFNGQEKP
                     QKGIVVKFKFQDGSKKFVRA"
     gene            complement(10816..>11041)
                     /locus_tag="PLU28_04530"
     CDS             complement(10816..>11041)
                     /locus_tag="PLU28_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HOG34364.1"
                     /translation="NGEIISYNISERPHLKQVFGLLKSELLYLREFESFEEFREELEK
                     YIYYYNHHRIKGKLKGLSPVQYRTQSFLIA"
BASE COUNT         3694 a         1760 c         2100 g         3487 t
ORIGIN      
        1 tctgcccata gtcgaaggga ctactgtttc aaccaagtat ggaccggtaa agacagattt
       61 catacttttt attgctgcag gggcatttca tgtcagcaaa ccatcagagc ttattcctga
      121 gtttcaggga aggtttccgg taagagttga actccagcca cttacagaag aggattttgt
      181 cagaatactt gtagaacccg agaatgctct cactaaacag tataaatctc ttctttggac
      241 ggacgatgtt gatataaaat ttgaagaatc tggaataaag tgtcttgcaa atatagcatt
      301 tacattgaac gataagattg aaaatattgg agcacgaaga ctttataccg taattgaaaa
      361 agttcttgaa gaagtttctt tccttgctcc ttcgggatca gcatggtcgg tttctattga
      421 tgaaaattat gtcaagaata gacttgacaa ggtggtagag gataagaatt tacgtgagta
      481 catactctga cggaggaaag tatggaattt tatgaggagt tgaatgaggt aataataata
      541 gaagattctg attctgtcat aaatctttat attcggctgt tatcaaactt tatgaaccgc
      601 aaggtcatgc ctagggttat aaagactttt caggaagtaa atacgatact ttcagaaagt
      661 tctaaactac ttagagagag agtttctctc ataattgccg aagcagaaat tgaagataga
      721 gaggttttgg atcagctttc catgataaag aaaactcttc ccaagaccaa ggtttatatc
      781 ataagcaata aggttaatat tgaaagactt aaaaaagcat taaaatcaga tctaagcgat
      841 tggattgaaa agccgattac tccagatgag ttttatgaaa ttgttaatat gtcattattc
      901 cgtggacaga cgtatgattc caaagctcga gaacttgata atgaaataaa agagtttgat
      961 cccgacaatg atcttaagta ctatactttg gaaaatatga tcagtaaact tatacttgag
     1021 aacccttcaa gatttgaagg tcattatctt ttaggaaagc tttatgaaag aagaggtata
     1081 aagactctgg ctcaaaaaca ttatgatgca gcacaggcat tgaaagatta atttgaggtg
     1141 tgtgatgaaa gaagctgtaa aatatatcat tattataggt tgtgggaggt taggatcaga
     1201 acttgcactt gaactttcat taaagcacag tgtagttgtt atagataaaa atgaagagtc
     1261 ttttgaaagg cttgcaaatc gtaattttac aggctttaca atgcttgttg atataaacga
     1321 tatggatgtg ctgaagaaaa tgaagttaga gaaagcagat atggtttatg ttgttacacc
     1381 tgacgataac cttaacttta tgctggcata cggtataaaa aagatgatgc cacatattaa
     1441 aattatggcc agggctaatg atcccatgaa aaaagaaata ttcatgcgcg aaggtatgga
     1501 gatattctgt cctatagaaa attcagtctc cggactggtt aaggattttt acgaggatga
     1561 taaacaatga gaaagaaact gttttatata ttcgaaggtc agactttggc atatgcttta
     1621 gcaaagaagc tgcttttagt tggtaatgaa gtattttata tttcaaagga cattaaaaac
     1681 attgaagtta tagacggttt aaaagccttt tttccatcgt tggagcttat tcaccacgat
     1741 cctactgata taaactgggt acagtcgctt gatatgtcca gtaatgtcgg agcggtaata
     1801 gtaatctctg aagatgatgc acttaacttt gtaataacat ggatgattag gcagctctat
     1861 gatgatgtaa gggttattgt ccttgttaat catccagaga atgagtttat gttcaaggca
     1921 ttacgtgtaa atacccttac tcctatatca tggatgcaaa agcttattga atcatttctg
     1981 aactatgacg atattacagc atattttaat ccatatatag aaaagctttc cattttggaa
     2041 cttatgatta ctaagaacga tagcgcctgt aataaaatgt tgaaagatct taatatgcct
     2101 ggtaattcta tcataggagt aattataaaa caggatggaa gtatagttgt tccaaaaggt
     2161 aatactgtag tacatgaaaa tgataaactt ctggttctgg cagctaaaga acaagtctct
     2221 acagtaaaag acattctaaa atagaaggtg aagttgtgcc tgattacatg ttttatttaa
     2281 aaatgcgtta taagagtgtt ttcagtgcat tagccaaagt aatgttcatg cagtctatac
     2341 ttatactgat tgtaggagct ttggcttttg ttttttcaca aggaaatcat ttttttccgt
     2401 ttttttatac atcactttta agttttttga taacagtaat ttttaaagta ttatcacgtg
     2461 gaaataaaac tgaaagtatg aatgttcagg atgctatgct tacggttttt tttatatgga
     2521 caatttcaat attagtctct gctatgcctt tttattttgg gaaaattctc gggttgactc
     2581 aatcaatttt tgaatctgca agtggctgga gtactacagg acttactgtt attgcagatg
     2641 ttgaatcaat tcctaaagtt tatcttttat ggagaagtat aatccagtat ataggcggtg
     2701 caggatttgc tttaatcatg attgttgtgg ccggtaacag cggtgctggt ctttatcagg
     2761 cagaaggacg tactgacaac cttgtgccca atatccgcga ttcagcaaaa ctgattcttg
     2821 tgatatatct tatctgggcc gtagccggca ttactgcact catgatcttt ggtaagcttt
     2881 ctttttttga tgcctttaac catgcactta cagctctagc taccggagga ttttcgacta
     2941 agaattccag cataggctat tttaataacg ttacggcaga gcttattatt atgattctta
     3001 tgatttcagg cgcaacagga tttggtgttc attattctct tgttcagctc ataaagaatt
     3061 attttaaaag caggaaagat ttaaccaatg gtaaaataaa cagacttgat tttaaagagc
     3121 gtatgaaagc agaaccgttt cttaagaatc ctgaacctaa aacaatgttt tatacgcttg
     3181 tgatatcgat aatactgtta gtcgttttgt gtacggtaca tatttattca ttacctcagg
     3241 cagtaagaca tagtgttttt actgcgatat cagctcttac tggtactgga tttcagacag
     3301 ttacgtttga aaaatggaat actctaggct tatttgttat tacaatccta atgatatttg
     3361 gtggaatgac cgattctaca tccggaggat taaaaacttt cagagtatat gtaataataa
     3421 aagtaatgat acttcaggta aaaaattatt tccgtcctca gggaacagaa catcatattg
     3481 aagtatacaa aggtgtagga aagaaaaaaa ttgatctggg tgtattcaaa gaagttattg
     3541 cagtggtatt tatgtatata attgtttttg ttacaggttc actgatactt gtcggacatg
     3601 gtttcagtct tgaagactct atgtttgaat tttcctctgc actttcggca gtcgggctta
     3661 cagttggtat tactgctcct gacgcaccgt catcaatttt atggacgctg attggaggaa
     3721 tgtaccttgg aaggtttgag tttttcctta tcttctatgc tgtgataagg cttatcaaag
     3781 atcttatgct gaaattaaaa aattagcctc ttaagaggct aattttttaa tttcttttaa
     3841 gcatgctgtt tatttcagga agtatatcaa acagggtaaa tttcttttct ccctttgctg
     3901 tttttacacg gttaaggatg agtatagaaa ttattctcag tagtatttcc aggaaaaata
     3961 taaactgtat gccaaagaaa agatgatcaa aaattacaaa agactttttt tgaagaaggt
     4021 ttccaagatt tccccctata aaagaggctg caattgcact gataccacat ataaaagcgt
     4081 taagtgaaaa atacatctct gaaggttctt tcgcagattc taaaagaagg ttaaaatatc
     4141 ccagattgat cgctgcccat gcaaacgaag taataaaagc atctaggaga gtcatcatta
     4201 tatagttatc tggtgtcata aaaagaaaga tgagaggtcc tgtaagagaa aaaattatac
     4261 ctattcttat agtattcttg tttccgaact tatcagagaa ctttccgtat aatatataaa
     4321 gaaacatcga taaaagggca cttatagttg atgcaagtcc caaaaaggta taatctgctt
     4381 taagaatggc aacctggtaa tatgaaaaaa aaggtttgca aaattcaata gaaaaattcc
     4441 aaaaagaagt gaataaaagg tattttcgaa aatctttatc tttaaatggt gtaaaaggat
     4501 tatcaatttt atcttttttt attagagtgt caggtatact atgttttgaa agcaagaaga
     4561 ctgatgagat aaagaatatg aacattagtg tacttacaat caacaaaccc agtttggtac
     4621 tgtcggtttt cattagataa gagtatacca ttgtaaaaac aactgtgatg atacttgtaa
     4681 tataatttct tagaccaaaa tacgaaccac gtacagaatc aggcacaatt tgcttcattg
     4741 cggatgtcca tacattacct gtaaaagaac tgaaaatact gaaaatcatc attacaacaa
     4801 aaagaatata agagtttgta aaatcaaata gtattgaaat aggtataaga actgttgcta
     4861 ttctggcaaa gaaagcagtt atgacaagaa gctcctttct gtttctgaat atgcggtaaa
     4921 gatatggaat aataatttga atcatcgaag cagcaagggg cattgtagtc attatcgaaa
     4981 gctgcaaagg tgttgcatta aagtatattg aaagtgtggt aaatataaaa ccctgtgttc
     5041 ctatataagc agcattgtat gtggcgcctt cagcaatcgc atacttcatt gtaggactca
     5101 tattcatttt ctttaaaaaa ttcatattat ctctcccaga aaaaatatcg tttatagtat
     5161 taaactattc aaatgtaaat tagtgattaa gccaagagag acgtactttg cagcaatatt
     5221 atatgtaaaa aattatagta agggttcaaa tcccatatcg aaccaataat tttgcattga
     5281 ttgcctgtaa agaccataga gtgcatccct atgagttttt tcccatttgg caagacttaa
     5341 aagtattttt ttcaggtctt tattgtccgt atagaaagaa gcttcttcat agaacttcat
     5401 aaaatcatct tcgatgagat atgccattct tattattgca atttccgaag tgtcaaagga
     5461 ttcgtggcct tgtggagtag tatatgagaa ttcggagttt ttccttgaat taaaaaaatt
     5521 atcatcagag gatagatcag ctattgctgt ggaattctct gaaatgtttc ctataagctt
     5581 atttataaat tctacatgtt catcttccat ttcggaaagc tctaaaaaaa gtttttttac
     5641 cgattcattc cgtatatcag atactttttg aagatagaag tttcttcctt caagttctct
     5701 tgatagtgca tactttaaaa ctcctataat tcccttttcc atcttattcc ctccctgatc
     5761 gttaataata aattatatca ttaattaata aaaataaaaa aacgtccaca agggacgtta
     5821 gaaaatatac taggggctct actcagtagt gtcagaaaga ttatactata tccgaactat
     5881 tttaaaaagt gacaaaagtc atatttctgt tcatttccag ctttgcatat actttttctg
     5941 atcctgagaa agtgagtcta tttttatgcc catggatgct agttttattg aagcaagctt
     6001 aatatcgata tcgtaaggta ttggtgagac attttttggc atagtagaat gattattcaa
     6061 gatatatttg gcaccttcaa gctgtaatgc aaaagactga tccattattt ctaccggatg
     6121 tccgtcagca ttaacaagat ttacgagtct accttttcca agaaggaaaa ccgaatgccc
     6181 gtttttcaga acacactctt ccactcctgg acggacttct gtgatacaat ccgatatctt
     6241 tttcaaatca gctaccttta cttcaacgtc gaaatgtcct gcattggcaa gaagtgcacc
     6301 atctttcatc tttagaaaat gcttctcggt tataatatca atatttcctg tagctgtaac
     6361 aaagatatct ccaagcaaac aagcttcatc cataggcatt actctgaaac catccataag
     6421 tgcttcaatt gcttttatct gatcaacttc agttactatt acgtttgctc ccagaccctt
     6481 tgcacgcata gcaatgcctt ttccgcacca tccatagcct cctataacta ctgtttttcc
     6541 tacaactgtc agattagtgc ttctcattat tccatcccag gttgattgcc cggtaccgta
     6601 tctattatca aaaagatact tcatgtatga gtcgtttacc gctattacag gaacaggaag
     6661 ctttccgcat ttttgcaaag aattatatct ttttacaccg gtagttgttt cttcgcagat
     6721 cccccataag ttattaagat cggaagaata gttctcaaaa aaacgtactc ccagatctgc
     6781 gccatcatct attataatgt ttggttttgc tgaaagtact ctgtcaatat ttttccaata
     6841 gaccgtttca tcaagtgttt tttctgcaaa cacatttatg ccgtactcct ttaaagccat
     6901 tactacatca ggctgcgtag taagaggatt gctcccggta ataaaaacat ttgcaccaag
     6961 ttccttcatt ataattgaaa gataagccgt tttggcttca aggtgtattg ccattgaaat
     7021 atttacatct ttaaatggtt ggctgtcttt gtatgaatct ctcaagaagt ttactatctt
     7081 catgtgcttt tctacccatt taatcttttg ccttccaagg ataacgtcca tcatgtacct
     7141 cctgtaaata tactgttttt tatcaattct gccagtagct gaaaccaacc attaaaggat
     7201 atggccaaaa atcgaaggaa agtccggagc ttcccttttc atagtcagac ataaccgtga
     7261 tacgtggatt gacagaaaaa gcgagtccaa aatcagcaag cggcctccaa atacttctta
     7321 tagaggtatc aggaaaacca gtcaaataat aagttgcagt aatatcaaga gcaggcgttg
     7381 gataccagtc ataaccgaag taatttattt taaagttttt tagtgggaaa agtacctctc
     7441 ctgaagcacc cagaccaaga taaaaggaac tatatgctcc taatgtctaa aatccgacgt
     7501 caaaacttgg tcctgcaact actataggag aagtctgatc cataatagtt atgttaagat
     7561 cggttcccat cttatagtat ccggaagctc ctgtataaat accccagaat ggccatagca
     7621 aaacatttcc accatagttt ttcctatcga agctaatgtc atttgcacat actgaagacg
     7681 ctaaaagtaa caaagccgat aacataacaa ataatttttt cattcctaaa cacctcccat
     7741 tatctataaa ctaattttac cacataacaa gagtataaag caatttaagt aatatataca
     7801 atacttgaac gacttatatt atatcatacg atataatata aaaactgtcg agagatataa
     7861 atatagaagt aaaaatgaag ttatgatata atttatacaa agaggtgaaa aaatggagca
     7921 ggcgcaagaa taccttcgag aaatacaaag tatatttaaa cgtgaattga ctgctgaaga
     7981 ggcgtctaaa attactgttg cctatgatct tgctcaaata tcccatcagg ggcagatgag
     8041 agcctccggt gaaccttttt ttgagcatcc taaagaagta gggcgtattc tggcaaaatt
     8101 taatatggat gtagatacta ttgcttcggg gcttttgcat gatgtatgtg aggattgtgg
     8161 agttccaatt gacgtaataa gagaaaagtt tggaaatgat gttgcccgta tagttgatgg
     8221 ggtaacaaaa atcagtaatc ttaagcttaa tgaaaagctt tcaaaggctg atactaaatc
     8281 tatagaaaag atagagactc tcaggaagat gcttttagct atgtctaccg atttgagagt
     8341 cataattgtt aagctttcag atagactcca taatatgcgt acacttgcat atacatctca
     8401 tgcgcagcag acaagtaaag ctcaggaaac acttaaaatt tatgctccga ttgctcatcg
     8461 tttgggaatt catactataa aagctgaact ggaggattta tcgtttttct atttgtatcc
     8521 agattcgtac aatgagttaa agatacgtgt cgataataag ctttcagata gacaaaaaag
     8581 tattgaagag tattcaaatt tattaaatgc tgaactagct aagcataata ttactgcaaa
     8641 ggtttacggg cgttccaagc atatttacag tatttgggat aaaatggttc gtaaaggaaa
     8701 aagctttgat gaaatttatg actttatagc tctgagaatt attactgaaa atcaaaatca
     8761 atgttatgca tctttgggga tagtacattc tctatggcct ccgataccag gcagaattaa
     8821 agattatatt gctactccta agcttaatgg ttataagtcg cttcatacta cagtcatcac
     8881 aaataaaggt gaacctcttg aaatacagat aagagactgg caaatgcacc aggaagcaga
     8941 atacggtctt gccgcgcatt gggcatacaa gatgggggta ggccacgaaa aaattaaatt
     9001 ccttcaaaac cttatggagc ttcataagga tattgctcag tctgcttttg atcttaatga
     9061 aattgagtcg gagcttgata ctgccgagac ttttgttttt actccacatg gcgagattat
     9121 acatttacct aaagatgcta ctcctataga ttttgcttat gcaatacata ctgatgtggg
     9181 taatcatttt gctggatcaa aaataaacgg taagattgtt cctatttctc ataagcttca
     9241 aaacggtgat gttgttgaga tcattatcaa tagaaacttt caggggccaa gcatggattg
     9301 gctgaagtat gctaggtctg caagaacaaa gagtaagatc aagaagtatt acagacagaa
     9361 aaatgaacag cttttgatag aacaggggaa ggataagttt agggaaatag caaaaaaatt
     9421 aggattctca atagaagatg tactcttaaa gctcaaggag agcgcctttt atctaaagtt
     9481 taacattaaa aacgatgagg atctatatgc taagctttca ttagatgatg tcaactatct
     9541 gactataaaa aattatgtgg taggtacaga agataagaag caatcctcta taaaaaacaa
     9601 aagttcgtct tccaagccaa ctgtattgat tcagggtatg gatggagtgg aaagttatta
     9661 tgccaaatgc tgtatgcctg ttccgggtga tgagattata ggtgttctaa gcaggcgtgg
     9721 aataggaata cacagaaaaa attgtaagat aataagggat gtatctgatg aaaaagttgt
     9781 aaaggtatcg tggaactcct acgatagcta tatctttacg gcaacattga atattgagat
     9841 gaaaagcaag gaagttatga attccataag aagcgttata accgctgaaa gagcttcgat
     9901 agagatgtat gagatggaaa gacagcttaa cagtcttatg acgaagatga gagtaaaggt
     9961 aaataatgta gagcatctta caagagtcat acaaaagatt tccgaggtta acggagtaag
    10021 cggggtaaga cgttcataag gagtggtata ttttgaggtg tgtagtacaa agagtcagtc
    10081 atgcgcaggt tgatgtagat aatataacta aaggttcaat aaataagggt ttaatgattt
    10141 tggcagcctt cagggatgcc gataaaaggg aggactttga atggatggcg gacaagcttc
    10201 ttaatcttag aatatttgaa gatgatcaag agaagatgaa tttgtctgtt acagatattt
    10261 cgggtgaact tctggtaata tcgcagttta ctttatatgg tgactgcagg aaagggcgca
    10321 ggccatcttt ttctgagagt gcaggactgg aaaaggcaaa aaaagattat gaagaatttc
    10381 tacaaatttt aacttccaaa accaatctta aggttgctac aggggttttt caggctgtaa
    10441 tgaaggtgag tcttgtaaat gagggtccgg tcacatttat catggattct tcaaaggtta
    10501 tataatatga atccgtaaaa cggattaaaa ggaggttttc cttatgcagg agacagaagt
    10561 cacagtattt atatattcgc aggagccttt tgaggcgcat atctctgcag aagctttgga
    10621 gaatgggata aatcaaacca atgttttaaa aattttcaat ggtcaagaaa aaccacaaaa
    10681 aggtatagtt gttaagttta aatttcagga tggaagcaaa aaatttgtca gagcataaaa
    10741 attagttgac tataaaaaac tggtaccata gaaaaataaa cttaccccaa aaagttagac
    10801 acatatggta ggacattaag ctattaaaaa ggactgagtt ctgtattgaa cagggctcag
    10861 tccttttaac ttgcccttga tcctatgatg attgtagtag tatatgtatt tttcaagttc
    10921 ctcgcggaac tcttcaaaac tttcaaactc gcgaagatac agtagctcag atttaagtaa
    10981 gccaaaaacc tgtttcagat gcggacgttc actgatatta tagctgatga tctcaccatt
    11041 a
//
LOCUS       DAOSHR010000057        11019 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_5003_length_11019_cov_5.032379, whole genome shotgun sequence.
ACCESSION   DAOSHR010000057 DAOSHR010000000
VERSION     DAOSHR010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 11019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11019
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5003_length_11019_cov_5.032379"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..757
                     /locus_tag="PLU28_04535"
     CDS             <1..757
                     /locus_tag="PLU28_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOG34365.1"
                     /translation="FKIGIPEIINYLSLAKEQSDFIKSIWNTSHYVILSTPITIFLAL
                     IIALLLNKSVKGKSFFRTLYFIPFVTSVVAVSLVFQWIFNDNGLLNYFLSFFGVERIQ
                     WLKNENWTIPTVAIISVWKLIGYYAVIFLSGLQSIDKSYYEAADVDGASGWHKLKYIT
                     WPLLSPTTFFILIVSMIGAFKVFSEIFVLYSGLPGPYNNSGLTVVYYVYDKFYNEQRM
                     GEASAAAYVLFGIILLFTFVQMAAGKNKVHYDS"
     gene            774..3005
                     /locus_tag="PLU28_04540"
     CDS             774..3005
                     /locus_tag="PLU28_04540"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOG34366.1"
                     /translation="MTYNKFWKTFGTVAIYLLTAAGALIMILPFAWMIITSFKSEGEV
                     NDWPPKWTSKNFYSSRELNVQTSRSNVSGKGNFNLAEFRTVSSEDPFESNKLVYYIND
                     DPVYRGKFELKFDTIDYSSSESLGIILQQMNDYISNTQVRDDLTSFFAVGENTPGFFE
                     ELYFNLFSPDDGYYKKIVMARLISSDISKMITYINRLNEGNIDKLVAFRISDSMSSQE
                     LTEVMAKKEVFSSDMAVLKEKLTEIKNILDNNYKGSEMLSNDDASALKTLLLDFASLI
                     NAYDDPVLSKNNQIMLNQFYNPAVKNVNTLEFLTYFTNSFKKVQSEKFSEQSIIFKVA
                     QKDKMYENFAADIKQADFPDFFKDEIGRVLTKDNILEIKDIVILQMEKEMTDNIRIKL
                     SSQPSDLSACISLVRDIAKLSPDEKEFENYLGENELKLYESFDDNKKKDLFELAGKRR
                     EYDSIFSRFGKFYVDSVSTADIVQAPSFVKTIRYKNNSIIEIITDNVSSTWLLEEKPL
                     VKATFTFGEVFKNIFQSYVIAWNAAPFSRYYLNTIFMALLTTFFEIILAAMAAFAFAK
                     LEFYGKNIIFSLFLSTMMIPGEVMLVPNYITISKFQWVDSYYALIVPWVVSVFAIFLL
                     RQQFLSLPNDLWDAAKIDGSSSWRFLWTVMVPLSKPSILTGGLLKFVGSWNAFLWVLI
                     VTKSPEMRTLAVGLQTFTTDAGTKYNLLMAASTFSILPVVIIFIFLQRYFVEGIARSG
                     LKG"
     gene            3009..3365
                     /locus_tag="PLU28_04545"
     CDS             3009..3365
                     /locus_tag="PLU28_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34367.1"
                     /translation="MPYIYISNTPKKAKRKSSRKLRRIIVLFFFIGLTVFFVYEISRQ
                     FSLYNDLKEKYDNLSQKYESLMNIYNEKQKILQDLEDKLKGKGVHFDGRSIKEFDIYN
                     IEQTEQSTDTTGTQLR"
     gene            3280..4059
                     /locus_tag="PLU28_04550"
     CDS             3280..4059
                     /locus_tag="PLU28_04550"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOG34368.1"
                     /translation="MDVQSKNLIFTTSNKPNSQQIQLARSYAEKYNGFYTDRKRIDFA
                     SLDESFCFVIEKDGSLKIRWKDGQFFYHPSVAKIKYSSYLFNKTDPLLKILSISSSDT
                     VVDLTMGLASEALFIAAFCKCVTGIEGSLPIYIIVSEGLINYGYEYEWMKQASKKVVC
                     VYSDLREYIRNQPNDSFDTVYCDPMFEHPKYSSSPINPLRKFAIYDPVTSEDVSHMLR
                     IARKRVVIKARSNDSLWERFKFSYTTDRRKSDISFGVIEKQ"
     gene            4056..4967
                     /gene="miaA"
                     /locus_tag="PLU28_04555"
     CDS             4056..4967
                     /gene="miaA"
                     /locus_tag="PLU28_04555"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOG34369.1"
                     /translation="MRVPIITGPTACGKTDLSIALAQWMGGEIICLDSRQIYKYLDIG
                     TAKPSTQDMEKVKHHLFGFVEPSISYSAYDYRNDALRCIKKILSSGRMPILVGGTGLY
                     IDCLIKGVIEVGSDYGLRKHLMKMENESPGSLKKILFSIDSQSGERIAENDIKRTIRA
                     IEIYILTGKKMSSLVNKNNVKSEFDFDIIVFDRDRKELYERIDKRTDDMLSQGMVMET
                     SELIRRYPESCNALNTLGYKQIIEYLKGRMGYEEAVNRIKFETHHYARRQIIYFRHFQ
                     NAKWINLSSKKNEEIKNELFDYIRRGK"
     gene            4970..5230
                     /gene="hfq"
                     /locus_tag="PLU28_04560"
     CDS             4970..5230
                     /gene="hfq"
                     /locus_tag="PLU28_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HOG34370.1"
                     /translation="MPEKFNLQDRFLNIIRTNKLEVKIYFDGGFQTSGQVKSFDNYTV
                     LIEKNGQQSLVYKHAIKMMVPSKYIKLFQQPAETPQENTEEN"
     gene            5238..6479
                     /gene="hflX"
                     /locus_tag="PLU28_04565"
     CDS             5238..6479
                     /gene="hflX"
                     /locus_tag="PLU28_04565"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOG34371.1"
                     /translation="MRIIGVIISIYRDANTVIEIAELAELCKSIGIQIHDHMTQKLIK
                     TDPKHYLGSGKIQEVKNLVIFTKSEIVIVNDRLSSTQKKNLEKLLCVQVMDRNEVILQ
                     IFRKNACSSESKCQVELASLQYELPKLIGKNDNLSRTGGGIGTVGPGETELEYNRRTI
                     QKRISVLKSKLKEIEAEHQRNRKNRLESSLPIISIVGYTSAGKSTLLKNISGDENILV
                     SPDLFSTLSPSVRRIKFACGLAAVVSDTVGFVNRLPLELIESFKSTLQEVKDSDIIVK
                     VIDLSSTCTAMHIRTIDSILGELNAQEIPQIIIFNKVDVSEQFKIDDYKLLYPKALFT
                     SAADPFNKSEILQFIQDSLIEKGIIKNNAVEVLSSEEWKFSKFIGNIGICEKKSIEEK
                     VYFKMLSKESQYKKIIKSLEV"
     gene            6485..7468
                     /locus_tag="PLU28_04570"
     CDS             6485..7468
                     /locus_tag="PLU28_04570"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOG34372.1"
                     /translation="MKKTCILIFLFLFGITLIGSVFTPPVRNSYITASFGEFRDTGDK
                     PHFHLGIDFSTFNREGINVYSAASGYVYKIWMNDPIYGNAVFIKHEDLNLITAYAHLQ
                     DFGDRIKYYSELIKKEFKGEGKIEAVFPKDQIQVQRSELIALSGSSGAAEAPHLHFEV
                     REANENGDIIRDPLEYLDYAETRTKALELLGVISDNAEYKPDSTNNVTIKFSGQYPKI
                     ELKVREVLGKNTPVMPKRISLKIGDMLIYQIDFSKILESESYNPSVVFGNSSTMTLYA
                     LKMFSTESITPIQVNNWNQFTMNSQNTFTGEVTLEDTWGNRQNYKINFVRN"
     gene            7542..8744
                     /gene="metK"
                     /locus_tag="PLU28_04575"
     CDS             7542..8744
                     /gene="metK"
                     /locus_tag="PLU28_04575"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOG34373.1"
                     /translation="MKGYLFTSESVTEGHPDKMADQISDAILDAILEKENPHNRINAR
                     VAVETLVATGMAVVAGQVRTDVYVDIPDIIRKTVIDIGYTRAKYGFDGETCAVLTSID
                     KQSDDIAMGVDKSLEAKEGKSHEDPYLKIGAGDQGIMFGYACNQTQELMPLPIMLAHK
                     LAYRMGQARKNKETEIFRPDGKTQVTVLYDENNKPVSIDTVLISTQHDPQASKIDIEN
                     AVREFVINPVIPKELLTKDTKILINPTGRFAMGGPQADTGLTGRKIIVDTYGGWIPHG
                     GGAFSGKDPTKVDRSAHYYARYVAKNIVAAGLADEVLIQLGYAIGVAEPLSILIDTKG
                     TAKVAAERLEKVVKEIFDFRPGAIIRNLDLLRPIYRKTAAYGHFGRNDSDFTWERTDK
                     VEDLKKAF"
     gene            8759..9043
                     /gene="rpsT"
                     /locus_tag="PLU28_04580"
     CDS             8759..9043
                     /gene="rpsT"
                     /locus_tag="PLU28_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOG34374.1"
                     /translation="MPNIDSAKKRVKQDEVRKERNKAYKSRFKNSIKKVLSAIEKKAG
                     KEELMTLLSEAFKSIDKAASKGAIHKNQAARRKSRLHIKVKKFLGEVAPQ"
     gene            9093..10031
                     /gene="trmB"
                     /locus_tag="PLU28_04585"
     CDS             9093..10031
                     /gene="trmB"
                     /locus_tag="PLU28_04585"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HOG34375.1"
                     /translation="MNTAALKYFTQPKTFDKFPISWNEVFGNKNKLVVEIGFGNGEFM
                     AHSAMKDTQFNYIGFDTSITSCYKAQVKLFDLGIENARIVLDDARFCIRELFSQESIH
                     KLVVNFPCPWPKAKQEDRRIFIYSFIDTMANVLKINGTVELVTDVQWYAEQVYEKFLS
                     DECFYVDEIKTDFQREIKTRYEEKWEKMGRNKYMVSVHKKASRSVQRLLEGSEDMPHK
                     LVEKIDLSKANDLLERKFSCEEQTFIVKEFFYNHEENRYIFRVFSADGDYCQQYYVKA
                     VKREDKYLVKLDSTTIPFRTPAVKWSVLTIADEISK"
     gene            10050..>11019
                     /locus_tag="PLU28_04590"
     CDS             10050..>11019
                     /locus_tag="PLU28_04590"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HOG34376.1"
                     /translation="MKITVLGAGSWGSAISKVLSLNGHDVRVWTREEKIYNAFLNGEN
                     PYYLPGITLPRTIKPFLEIDKSIEGSEVIVNAIPAQFTRSVMKQVGDIGKKLFINLAK
                     GIEIESGMLINQIFFQETKSEFYCTLSGPSHAEEVARDIPTTVVACSKNVSTAEFVQK
                     IFSNASFRVYTNSDVSGVEMCGALKNIYAIACGIIDGFGTWDNSKAALITRSMVELAR
                     YGENNGAKKETFFGLAGIGDMIVTCTSLHSRNRHVGEMIGKGKSLNEIIESMNQVAEG
                     VYTSKALHRILKEKYTEMPIAEKIYSILYESYDPKKAISDLMNRELK"
BASE COUNT         3869 a         1614 c         2187 g         3349 t
ORIGIN      
        1 ttttaaaata ggaattcctg aaataataaa ctatcttagc cttgccaagg agcagtccga
       61 ttttataaag tcaatctgga atacttccca ttatgttatt ctctcgacac ctataacaat
      121 ctttttagct ttaataatag ctctgttgtt gaacaaaagt gtaaagggta aatcattttt
      181 tcgaacgttg tactttatac cttttgttac gagtgtggta gcagtttcgt tggtttttca
      241 atggattttt aatgataatg gacttctgaa ctatttttta tcattttttg gtgttgaaag
      301 gatacagtgg ctgaaaaatg aaaattggac cataccaaca gttgcaatta tttcggtatg
      361 gaaacttatt ggatactatg cagtaatatt tctttcaggc cttcaaagca ttgacaaatc
      421 atattatgaa gcggctgatg ttgatggtgc aagcggatgg cacaaactta agtatataac
      481 ttggccttta ctttcaccta caactttttt tattcttatt gtatctatga taggtgcatt
      541 caaagttttt tcggaaatat ttgttcttta ctccggactt ccgggaccat acaataactc
      601 tggacttaca gttgtttatt atgtttacga taagttctac aatgaacaaa gaatgggaga
      661 agcaagtgct gcagcatatg ttctttttgg tataattctt ctttttacat ttgttcagat
      721 ggctgccggg aaaaacaaag ttcattatga ttcttagtaa acggaggtaa ataatgacat
      781 ataataaatt ttggaaaact tttggtacag tagcaatata tcttctaacg gctgccgggg
      841 cacttataat gattcttcct tttgcatgga tgattataac ttcttttaaa tctgagggag
      901 aagttaatga ctggcctcca aaatggacat cgaaaaattt ttattcttct cgtgagctga
      961 atgttcaaac ttcaagatca aatgtttctg gaaaagggaa cttcaatcta gcagagttcc
     1021 gtactgtttc ttcagaagat ccttttgaaa gtaataagct agtctactat ataaacgatg
     1081 atcctgtcta cagaggaaag tttgagctta agtttgatac aatagattac agttcttcgg
     1141 aaagtcttgg aattatttta cagcagatga atgattatat atccaacact caggtaagag
     1201 atgacctgac ttcttttttt gctgttggag aaaacactcc tggttttttt gaagagctat
     1261 acttcaatct tttttctcca gatgacggat actataaaaa aattgttatg gcaagactta
     1321 tatcttctga tatttctaag atgataacgt atatcaacag attgaatgag gggaatatag
     1381 ataaacttgt tgcttttaga atatctgaca gtatgtcttc acaagagtta actgaagtta
     1441 tggctaaaaa agaagttttt tcttctgata tggcggtact taaagaaaaa cttactgaaa
     1501 taaaaaatat tttggataac aactataaag gttctgaaat gcttagtaat gatgatgcat
     1561 cagctttgaa aactcttttg cttgattttg cgtctttgat caatgcatat gacgatccag
     1621 ttctttcaaa gaacaaccaa attatgttga accaattcta taatcctgct gttaaaaacg
     1681 tcaatacatt ggaatttcta acctatttta ccaattcttt taaaaaagta cagtctgaaa
     1741 aattctcaga gcagagcata atttttaaag ttgcacaaaa agataagatg tatgaaaatt
     1801 ttgcagccga tattaaacaa gcagattttc cagatttttt caaagatgag ataggcaggg
     1861 tacttacaaa ggataatatt ttggagataa aagatatagt tatccttcag atggaaaaag
     1921 aaatgaccga taatatcaga attaagcttt catcacagcc atctgatctt tctgcatgta
     1981 taagtcttgt acgtgacatt gcaaagcttt ctccagacga gaaggagttt gagaattatc
     2041 taggtgaaaa tgaacttaag ctgtatgaat cttttgatga taataagaaa aaagatttat
     2101 ttgaacttgc cggaaaaagg cgtgagtatg acagtatttt tagtagattt ggaaaatttt
     2161 atgtcgattc cgtatctact gctgatatag ttcaggcacc atcatttgtc aagacaatac
     2221 gatacaagaa taattctata atagaaataa taaccgacaa tgtttcatct acatggcttt
     2281 tggaagaaaa accgttggta aaagctactt ttacttttgg agaggttttt aaaaatattt
     2341 ttcaaagcta tgttatagct tggaatgcag cgccattttc cagatattac ctaaacacca
     2401 tttttatggc attacttacg actttttttg aaattatact tgcagcaatg gcggcctttg
     2461 cctttgccaa gcttgagttt tatgggaaaa acattatatt ttcacttttt ctgtctacta
     2521 tgatgattcc cggagaagta atgcttgtgc ctaactatat tactataagc aaatttcagt
     2581 gggttgattc gtattatgct cttatagtgc catgggtagt cagtgttttt gcgatatttc
     2641 tactcagaca gcagttcctt tcactaccca atgatttatg ggatgctgca aagatagatg
     2701 gttcttcaag ttggaggttt ttatggaccg taatggtgcc tttaagcaag ccttccatac
     2761 ttactggtgg tctattgaaa tttgtgggaa gctggaatgc ttttctttgg gtacttatag
     2821 taacaaaaag tcctgaaatg cgtactttgg ctgttggcct tcagacattt acaaccgatg
     2881 caggaacaaa gtataacctt cttatggcag catcgacttt ctcaatactc ccagtagtca
     2941 taatttttat attcttgcaa agatactttg ttgagggtat agcaagaagc ggtctgaaag
     3001 gataatcaat gccgtacatt tatataagca atactcctaa aaaagccaaa agaaagagtt
     3061 ccagaaagtt aaggcgtata attgttctct ttttctttat aggattaact gttttttttg
     3121 tgtacgaaat atctagacag ttttctttat ataatgattt aaaagaaaaa tatgataatc
     3181 tttcgcaaaa atacgaaagt cttatgaata tttacaatga aaaacagaag atacttcagg
     3241 atcttgaaga caaactcaag ggaaaaggag ttcattttga tggacgttca atcaaagaat
     3301 ttgatattta caacatcgaa caaaccgaac agtcaacaga tacaactggc acgcagctac
     3361 gctgaaaagt acaatggttt ttatacagac agaaaacgaa tagattttgc atcccttgat
     3421 gagagctttt gttttgtaat agaaaaagat ggttctttga aaataagatg gaaggatggt
     3481 cagttttttt atcatccatc ggttgctaaa ataaagtatt caagttatct gtttaataaa
     3541 acagatccgc ttttgaaaat attaagcata tcaagcagcg atacagttgt tgatcttacc
     3601 atgggattgg caagtgaagc gctttttata gcagctttct gtaaatgcgt tacaggaatc
     3661 gaaggttcat tacctatata tataattgtc agcgaaggct taattaacta tggttacgaa
     3721 tatgaatgga tgaagcaagc gtcaaaaaaa gtagtctgcg tttactcaga tctgcgtgaa
     3781 tatataagga atcaacccaa tgattctttt gatactgtat actgtgatcc tatgtttgaa
     3841 catcccaagt actcttcgtc accaatcaat ccgttaagaa aatttgcaat ttatgatcct
     3901 gtgacttctg aggatgttag tcatatgctc agaatagcac ggaagagagt agttattaaa
     3961 gccagaagca acgacagtct atgggaaaga ttcaaatttt cttatactac tgacagaaga
     4021 aaaagtgaca tctctttcgg agtaatagaa aaacaatgag agtaccaata atcacgggac
     4081 ctactgcctg tggaaaaact gatctttcta tagctctagc tcaatggatg ggtggagaaa
     4141 taatctgtct tgattccaga cagatatata agtatttgga tataggaaca gctaaacctt
     4201 ctacacaaga tatggaaaaa gtaaagcatc atctttttgg atttgttgag ccatccatca
     4261 gctatagtgc ttatgattat aggaatgatg ctttaagatg tataaaaaaa atcttaagtt
     4321 caggacgtat gccgatactt gtaggtggta caggattata tatagactgc ctcataaaag
     4381 gtgtaattga ggttggctcg gactatggac taaggaagca tcttatgaaa atggagaatg
     4441 aatctcctgg cagcttaaag aaaatacttt tttccattga ttcacaaagt ggggaacgga
     4501 ttgctgaaaa cgatataaaa agaactatca gagcaataga gatatatata cttacaggta
     4561 aaaagatgtc aagtttagtt aacaaaaata atgttaaaag tgagtttgat tttgatataa
     4621 ttgttttcga tagagacaga aaagagctgt atgaaagaat agacaaaaga acagacgata
     4681 tgttgagtca aggtatggtc atggagacat cagaacttat aagaagatac cctgaatcat
     4741 gtaatgcact caacacctta ggatacaagc agattataga gtatcttaaa ggcagaatgg
     4801 gttatgagga agctgtaaac agaataaaat ttgaaactca tcattatgcc aggcgtcaga
     4861 taatttactt cagacacttt caaaatgcca aatggattaa tctttcttca aaaaagaatg
     4921 aagaaattaa aaatgaactt tttgattaca ttagaagggg gaaataatta tgccggaaaa
     4981 gtttaatctt caggataggt ttttaaacat tatacgtaca aataagcttg aagtaaagat
     5041 ttattttgac ggaggttttc agaccagtgg acaggtaaaa tctttcgata actatactgt
     5101 cctcattgaa aagaatggtc agcagtcgtt agtgtacaag catgcaataa aaatgatggt
     5161 accttccaag tacataaagc tttttcagca gccagctgaa acgcctcagg aaaatacgga
     5221 agaaaattaa ggagtgattg agaatcatag gagttataat aagcatctac agagatgcta
     5281 atactgtaat agagattgca gagttagcag aactatgcaa aagtattggt atacagatac
     5341 atgaccatat gacacagaag ctaataaaga cagatccaaa acattattta ggttccggaa
     5401 aaatacaaga agtgaaaaat cttgtgatat ttacaaagag cgaaatagtg attgtaaatg
     5461 acagacttag cagtacacag aaaaaaaatc tggaaaagct tttatgtgtt caggtgatgg
     5521 ataggaatga agttattctt cagatattca ggaaaaatgc atgttcatca gaaagtaaat
     5581 gtcaggttga acttgcgagt ctgcagtacg aactgcctaa gctgataggg aaaaatgata
     5641 atctttccag aaccggtgga gggattggta ctgttggtcc aggagaaacc gaacttgagt
     5701 acaatagaag aactattcaa aaacgtataa gtgttcttaa gtctaaactt aaagaaattg
     5761 aagctgaaca tcaaaggaac agaaaaaata gacttgaatc atctttgcct ataatatcta
     5821 tagtaggcta tacttctgcc ggaaaatcta cattactgaa aaatatttca ggcgatgaga
     5881 acatacttgt atcgcctgat ttgttttcta cactctctcc ttcagtaagg aggataaagt
     5941 ttgcttgtgg gcttgcggct gtagtgtctg ataccgtagg ttttgtaaac aggttgccac
     6001 ttgaactgat agagtctttt aaatcgacac ttcaagaggt aaaggattcg gatataatag
     6061 taaaagtaat agacttatcc tcaacatgca ctgctatgca cataagaact atagatagta
     6121 tacttggaga gcttaatgca caggagatac ctcagataat tatttttaat aaagttgatg
     6181 tttcggagca atttaaaata gatgattaca agttacttta tcccaaggct ttgttcacga
     6241 gtgcagcgga tccatttaac aaaagtgaaa tacttcagtt tatccaggac agtttaattg
     6301 aaaaaggcat aataaaaaac aatgctgttg aagttctttc cagtgaagag tggaaatttt
     6361 cgaagtttat aggtaatata ggtatatgtg aaaagaaaag tatagaggaa aaagtttact
     6421 ttaaaatgct cagtaaagaa tctcagtata agaaaataat caaatctttg gaggtgtaga
     6481 agtcttgaaa aaaacatgta tactaatttt cttattttta ttcggaatca cactaatagg
     6541 gtcggttttt acacctcccg taagaaattc ttatattaca gcttcttttg gtgagttcag
     6601 agatactggc gataaacctc attttcatct aggcattgat ttttcaacat ttaacagaga
     6661 gggaataaat gtttattccg cagcatcggg atatgtttat aagatatgga tgaacgatcc
     6721 tatatatggt aatgcggttt tcataaaaca cgaagatctt aatctaataa ctgcatatgc
     6781 tcaccttcag gattttggag atcgtataaa atattactcc gaactaataa aaaaagagtt
     6841 taaaggagaa ggtaaaattg aggcagtatt tccaaaagat cagatacaag tccagagaag
     6901 tgaactaata gctctttctg gaagttcagg tgctgctgaa gcgccccatc ttcattttga
     6961 agtcagagaa gctaatgaaa atggcgatat tatacgagat cctcttgagt atcttgatta
     7021 tgctgaaaca cgtacaaaag cactggaact tttaggggtc atttcagata atgctgaata
     7081 taaacctgat tcaactaata atgttacaat taaattttct ggtcagtatc ctaagataga
     7141 actcaaagta agagaagtac taggtaagaa tactcctgtt atgccaaaac gtatatcttt
     7201 gaagataggg gatatgctta tttatcagat tgatttttcg aaaatacttg agagtgaatc
     7261 ttacaatcca tctgttgtat ttggaaattc atcaacgatg acactttatg cattaaaaat
     7321 gttttcaacg gaaagtatta ctccgattca ggtaaataac tggaatcagt ttacgatgaa
     7381 cagtcaaaac acttttaccg gagaagtaac tttagaggat acttggggaa atagacagaa
     7441 ttacaagatc aattttgtta gaaattaaac tgttactgtt ttttaacctt aaatgatgta
     7501 aaatatttga gtagtgatta tttatattta ggaggtctac tatgaaggga tatttgttta
     7561 caagcgaaag tgttacagaa ggacatcctg ataagatggc cgatcagatt tcagatgcta
     7621 ttcttgatgc tattcttgaa aaagaaaatc ctcacaatag aataaatgca agggtagctg
     7681 tcgagactct tgtggctacc ggtatggccg ttgtggccgg tcaggtgaga acggatgttt
     7741 atgttgatat acctgatata ataagaaaaa cagttattga tatagggtat accagggcaa
     7801 agtatggttt tgacggagaa acctgtgctg ttcttacatc tattgataag caatccgatg
     7861 atatagctat gggagttgac aaatctcttg aagccaaaga gggaaaatct catgaagatc
     7921 cttatctaaa aataggagca ggtgaccagg gaataatgtt tggatatgcc tgcaaccaaa
     7981 ctcaagaatt aatgccattg ccaataatgc ttgcacataa gctggcttac agaatgggac
     8041 aggcaagaaa aaataaagaa acagaaatat ttcgtcctga cggaaaaacg caggttacgg
     8101 ttttatatga tgagaacaat aagcctgttt ctatagacac tgtgcttata tctactcaac
     8161 acgatcctca ggcctcaaaa atcgatattg aaaacgctgt aagagaattt gtaataaatc
     8221 cagttattcc aaaagaatta ttgacaaaag atactaaaat tttgattaat cctaccggaa
     8281 gatttgctat gggtggtcct caggctgata caggtctgac aggcagaaag attatagtag
     8341 atacttacgg agggtggata cctcacggag gcggagcatt ttccggtaaa gatcctacaa
     8401 aagttgacag atctgcacat tattatgcga gatatgttgc aaaaaatatt gtggctgcag
     8461 gtcttgccga tgaagtactg atacagcttg gttatgcaat tggcgttgca gaacccttat
     8521 ctatactcat tgacactaag ggaacggcta aagttgcggc agaaaggctt gaaaaagttg
     8581 ttaaagagat ttttgatttc agaccgggag caataataag aaatcttgat cttttacgcc
     8641 caatttatag aaaaacagcc gcttatggtc actttggaag aaacgattca gactttacct
     8701 gggaaagaac cgataaagtt gaggacttaa agaaagcatt ctaaaaggag gtaacaaagt
     8761 gccaaacatt gattcagcaa aaaagagagt caaacaggat gaagttagaa aagaaagaaa
     8821 caaagcttat aagtcaaggt ttaagaactc aataaaaaaa gttctttcag caattgagaa
     8881 aaaagctgga aaagaagagt taatgacact tctttccgaa gcctttaaaa gtattgataa
     8941 agctgcttca aaaggtgcta ttcataagaa tcaggcagct agaaggaaat caagacttca
     9001 tatcaaagtt aaaaaatttt taggtgaagt agctccacaa taaaaatgaa tactcccggc
     9061 atatgccggg tgttttttta agggtgaata tcatgaatac agcagcttta aaatatttta
     9121 cccaacccaa gacttttgat aagtttccta tcagttggaa tgaagttttc ggaaacaaaa
     9181 ataaactggt tgtagaaata ggttttggga acggtgaatt tatggctcac agcgccatga
     9241 aagatactca atttaattat ataggctttg atacttcaat aacctcatgc tataaagctc
     9301 aggtcaagct ttttgatcta ggaatagaaa atgctagaat agtactggat gatgccagat
     9361 tttgtattag agaacttttt tctcaggaaa gtattcataa attagtcgtg aattttcctt
     9421 gtccttggcc taaagctaag caagaggata gaagaatatt tatttattca tttattgata
     9481 caatggcaaa tgttcttaaa ataaatggga cagttgaatt ggtaacagat gttcaatggt
     9541 atgctgaaca agtctacgaa aaattcttat cagatgagtg tttttatgtt gatgaaataa
     9601 aaactgattt tcaaagggaa ataaaaacga gatatgaaga aaaatgggaa aaaatgggaa
     9661 ggaataagta catggtttcg gtgcataaaa aagcttcaag gtcagttcaa agattattag
     9721 agggaagtga agacatgcca cacaagcttg ttgaaaaaat tgatctttca aaagcaaacg
     9781 atctgttgga aagaaaattt tcttgtgaag aacaaacatt tatagtaaaa gaattttttt
     9841 ataatcatga ggaaaacaga tatattttca gagttttttc agcggatggt gattactgtc
     9901 agcagtacta tgtaaaagca gtaaaaagag aagacaaata tctggtaaag cttgattcaa
     9961 caacgatacc tttcagaaca cctgcagtta agtggtcagt attgactatt gcagatgaga
    10021 tcagcaagta aaaaaggagg cgagaagaat tgaagataac ggttcttggc gctggaagct
    10081 ggggaagcgc aatttctaag gttctttcct tgaacggaca tgatgtaaga gtttggacaa
    10141 gggaagaaaa aatatataat gcttttctta acggtgaaaa tccttattat cttccgggaa
    10201 taactcttcc aaggacgatt aagccatttt tggaaattga taagagtata gaagggtctg
    10261 aagtaatagt aaacgctatt ccggcacagt ttactagaag cgttatgaaa caagttggag
    10321 atataggaaa aaaactgttt ataaatcttg ccaagggaat agaaatagag agtggaatgc
    10381 ttataaatca gatttttttt caggagacaa aatcagaatt ttattgtaca ctaagcggtc
    10441 cgagccatgc agaagaagtt gcgagagata ttccgaccac agttgtagca tgcagtaaaa
    10501 atgtatctac agcagaattt gtacaaaaaa tatttagtaa tgcatccttt cgtgtgtata
    10561 caaacagtga tgtatcagga gtagaaatgt gtggtgcatt gaaaaatatc tatgctattg
    10621 cctgtggcat aattgatggt tttggaacat gggataattc caaagctgct ttgataacca
    10681 gatctatggt agaactggcc agatatggcg aaaataatgg ggcaaagaaa gagacttttt
    10741 ttggtttggc aggtattggg gatatgatcg taacttgtac ttcccttcat agcagaaaca
    10801 gacacgtggg agaaatgata ggcaaaggaa agtctctcaa cgaaataata gagagtatga
    10861 accaagttgc cgaaggagtt tatacttcaa aagctttgca taggatatta aaggaaaaat
    10921 ataccgaaat gccaatagct gagaaaatat acagtatact atacgagagt tacgatccta
    10981 aaaaagctat aagtgattta atgaacaggg aactaaaag
//
LOCUS       DAOSHR010000058        10880 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_5080_length_10880_cov_5.825035, whole genome shotgun sequence.
ACCESSION   DAOSHR010000058 DAOSHR010000000
VERSION     DAOSHR010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 10880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10880
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5080_length_10880_cov_5.825035"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1268
                     /locus_tag="PLU28_04595"
     CDS             <1..1268
                     /locus_tag="PLU28_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34377.1"
                     /translation="IDGYIYITTYRYLLDFVELCRTGVIKAYNFADKYAEYAVSYHTS
                     YFKPLSSVFHYLGDIYGKELGEEYHLKLTQEGVYAEILINKEIEEYEKLYLKADFLDD
                     LSVLKDADIVVSMKKEYFDIIRSITNENEPAMEYLKHLVQERAVLSVKGTISGIDDIS
                     SIEKLYLSFYLSKENAEIVKNEFEQALSMSFMSDGENYIIFDQDRNIFLSLKIGGGIG
                     QLKYCKDASDFFETEKILEKASTSKLEDFLYYEDENSRVSFIKVFSDESSFKKICLSV
                     SYDHIINFLTEIIMSAFITKDTEDEIPEIDWDRYSEDDKNDFGWKLFDLFSAINVLKN
                     SDETVVLDMESLYNKDLLNYELYELTDFSVRKFMDYEGQETFEIFYKGNTAGYINDYE
                     IFDYLYSSSEYLTVEIDRDNLTVFVRWTK"
     gene            complement(1298..2410)
                     /locus_tag="PLU28_04600"
     CDS             complement(1298..2410)
                     /locus_tag="PLU28_04600"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG34378.1"
                     /translation="MNNRFLNLVKFFFLETFKNKSEVFYTMFFPVIFLLLFGFIFGLG
                     DTQHQEENFEYGLVFSSGYLSDTNQSSESAYETVKAFFEPNKLDIYADNESLKNAVLS
                     SKVDAGIIVDETSKVKVLYNSDPANMQKITWIKFAGENAIKRFLYGAKKDYLSLSSEN
                     IGTGRVESNQMDFLLTGIIAISILSGGMFSVINTFGRYKKLGVIKKFMVTPIKSNEFV
                     IASSFNKILLNFFSIVIIIILGKVIYHMNYSFDWPMLIIVCLSSTLGMMAFGILLMVL
                     FKELEVAQSAASILMTVMMFFAGIYFPINIIPGYIRWVSYILPIKYVADLLRHVAGVE
                     SMNITTFWLINIIMAASGIILLSLTSRQLMKDQARN"
     gene            complement(2403..3557)
                     /locus_tag="PLU28_04605"
     CDS             complement(2403..3557)
                     /locus_tag="PLU28_04605"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG34379.1"
                     /translation="MKIKSLLSIFLKEDFRNFIVIFWTFVFPLIFFLFFTSLFGNIGN
                     GSENSSDMEFRAGIYFEKPPTGLAKEISQGLVTGLGDKNSGGPFENIYVSSFDEGIQL
                     IKDKKLEIFIVFPENLDMLNLKVVLSSVPAPVVKSYYRSDSGSDFARKIFKSVLDSVN
                     VEFYKRISSAEYTLEVQELNKKASDTFSYKDYLFPSVLLMSILTVSIFNTPLSLAYSI
                     EKGITKKIFTTPIRSGHFLTVVILENLILLGISILLIYIEGKLMGVSPKAFSAKYIAY
                     LFMNVITLISVGLLMLSVFRKLSAVNVAANILYQLMMFLGGLFFDVSHTPMIIRWFVY
                     INPVSYMAQGLRNIINGTPLGFLNYFVPLLWIGACIIIFSLNFKKVMNYE"
     gene            complement(3566..4492)
                     /locus_tag="PLU28_04610"
     CDS             complement(3566..4492)
                     /locus_tag="PLU28_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG34380.1"
                     /translation="MENIIEVSNLRKYYKTVKAVDDISFSIAAGKVFALLGPNGAGKT
                     TTVEILEGLRRADSGRITYFGKEITKVNFELKEKIGVQLQNTVFFESLTVKETLKGFS
                     GLYEKKFDIDILVKKLELEEKLNSKIKTLSGGQKQRLAIAVALVNDPDIVFLDEPTTG
                     LDPQARRMLWDFINELKSSGKTIILTTHYMEEAEYLSDFVNIVDHGKVIACGTVEQLI
                     DSIGMKSIIRFHSNKKFDSYFKDENLKIIEDTALSSEKKIYEIQTDNVETTLSNIFET
                     TRKNSLSISEIAIRQPNLEDVFLKLTGRTLRD"
     gene            complement(4673..5722)
                     /gene="mtnA"
                     /locus_tag="PLU28_04615"
     CDS             complement(4673..5722)
                     /gene="mtnA"
                     /locus_tag="PLU28_04615"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HOG34381.1"
                     /translation="MSRIKGMSMEWSDFNLIFIDQRKLPLEEVYVQCTTYTQVATAIK
                     DMIVRGAPAIGASAAFGYVLGAKEFEKKTKDSFILKMAEVKNALAATRPTAVNLFWAL
                     ERMEGKMKTVILENDTYETVKILEDEAIAIAQEDIKVNQALGEYGAQLLKDGDTVLTH
                     CNAGALATFDFGTALGVIRAAVKSGKKIKVYADETRPYLQGARLTVWELMKDHIDVTL
                     ISDNMAGWVMHKGLINAVIVGADRIAANGDTANKIGTYSVSILAKQHSIPFYIAAPVS
                     TIDIKSESGKDIPIEERDHNEVRYCHKLKMVPDSVKVFNPSFDVTPHANISGIITEKG
                     VLRTPYDLSIKKIFL"
     gene            complement(5737..6543)
                     /locus_tag="PLU28_04620"
     CDS             complement(5737..6543)
                     /locus_tag="PLU28_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HOG34382.1"
                     /translation="MENPVNAMIEKAQNTMKNISHKIVVMSGKGGVGKSTVAVNLAVA
                     LALEGRKVGLLDIDLHGPDVVRMLGGNSAKPYQVGNDVLPPEINGIKVISTSQFLKDE
                     TSPIVWRGPLKTSAILEFLGDIAWGELDYLIMDCPPGTGDELLTVFQNFKDIDGTLIV
                     TTPSVVSQDDVEKAINFSKMMNVNILGLVENMSYFVCEKCGEKHYIFGQDGGKTLAEK
                     YNLELLQEIPINKIIRSNMDKGQPAAYFGIPDTVAPFINLAKKIIGKTEK"
     gene            complement(6562..6990)
                     /locus_tag="PLU28_04625"
     CDS             complement(6562..6990)
                     /locus_tag="PLU28_04625"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOG34383.1"
                     /translation="MTKEYLYEYAGKREFYSRTPSGHDLFTDATIESGGNGRAATPVE
                     MVLSGLAGCTGIDVTMILEKKRVSFDSLKIRMITERTDDEMRHFEKIHLIYEFSGHDL
                     PLGKIQQSVQLSQDKYCTVAAMLKGSIKLSYEIKLVETQK"
     gene            complement(7038..9194)
                     /locus_tag="PLU28_04630"
     CDS             complement(7038..9194)
                     /locus_tag="PLU28_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34384.1"
                     /translation="MNILNTFLTQSSNASQLTELAAKNGKITGKVIYSDGEINLVSIN
                     GKLYILNSEDGKKLPEGSLVNIVFSKNTIPSTDQNIIKSYITKALGEISGNIIKFSIG
                     LTQETNQSILSNIKIDFSEIPDAQKGKFLVWISDLFDSLNTSMQNDKAFSSVLEDFSR
                     KEVFSGFIKSMISELSKDFFSNKIFTADPRFISEKAVTLFKDSYLSSKQSEDIKPTNL
                     FNSSDIDKNESPLIQSNFEQEDKMVDKNFLIKKEPLQESSHEKKLDSLKDHAPDKKQT
                     YKIENQVKTLLSQKDDLPFTKNEKIKSQDSPQIARNTYKSDIPKEAENHFQKRSLDFQ
                     SAKNTFKKEPFDESLIQRNSNQLKKELILDYFKNHYSAAKKKNSEPAAFHKAKVISEV
                     LTEKSATDLSLKKTEPAENLSQKIDLATFKLYKSVLRDIFDSGNNIRTVYISKQKNDA
                     ITNFFRKESTFEVNNSEQSSQQKPISEKLSHNVSKTIFEKASSLTTESRKQPDMIEEI
                     PVKNSFNVPQKKIIETSHQTDQNTELKNDSRHATVLGKVKISDSFRNELLAEISAEKK
                     VSEKLSFEDNGLFSKNSISTAVEKAVSVYKMFENTKTTEASTYFLVSFLGIPVFFNFR
                     RLSDDFGNEKNSVEKNYGKLRMVLPTETFGMADINLFMSKNDVIIKLGIDRNTKYMQS
                     NSVSLNQALENIGYNLVLFDIFINPQNPDISRDTLI"
     gene            9363..9695
                     /locus_tag="PLU28_04635"
     CDS             9363..9695
                     /locus_tag="PLU28_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34385.1"
                     /translation="MGIFKKIFRITDHENKKESQVFYSEGNSLVVYFRCKKCGEKFAA
                     YLRKGYDLITNYDDSQASYFIDKEFIGSKCFEKINLSAEFDERYKIIKLNLTGADILT
                     RQEYEKDD"
     gene            9688..10194
                     /locus_tag="PLU28_04640"
     CDS             9688..10194
                     /locus_tag="PLU28_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34386.1"
                     /translation="MTDNFDTDRSIDVFEYRPVRPFIQIPEKVMVYVLGLTPSSFSKV
                     YLYIYNLSYGLGRDYTQAFTSVREISSNLNISIGRTQNAMAYFKKINAFVKIVSKAVI
                     GSKYKVNVPEYSAQSGSWYFVEPVKVKYLLLKEKLITSDSIVESKIKRYKSIRESLKI
                     GLFDNPEE"
     gene            10437..>10880
                     /locus_tag="PLU28_04645"
     CDS             10437..>10880
                     /locus_tag="PLU28_04645"
                     /inference="COORDINATES: protein motif:HMM:NF014919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching enzyme"
                     /protein_id="HOG34387.1"
                     /translation="MDNSSIKISRGHPKLGATVDPSGTNFAVFSRNGKRVFIELYENF
                     YDDIPFFSYELDRIYNKTGDIWHICLFGIGNNLYYLWRVDGDYEPLSGHRFNKNKVLL
                     DPYAKAISGVYDFDDPSMYSYDTSDDSNMDLSFSRTNSVRSACKNI"
BASE COUNT         3488 a         1842 c         1858 g         3692 t
ORIGIN      
        1 atattgatgg ttatatatat ataacaacct acagatatct tcttgatttt gtggagctct
       61 gtcgcacagg agttataaaa gcttataatt ttgctgataa gtatgcagag tatgcggtaa
      121 gttaccatac gagttatttt aaaccgttat cttctgtttt tcattatctt ggggatattt
      181 acggaaagga gcttggcgag gagtatcatt tgaagcttac gcaggaagga gtatatgcag
      241 agattttaat taataaggaa attgaggagt atgaaaaact atacttaaaa gcagacttct
      301 tggatgacct gtcagttcta aaggatgctg acattgtggt ttctatgaaa aaagagtatt
      361 ttgatattat aagaagtatt acaaatgaga atgagccagc tatggagtat cttaagcatc
      421 tggtacagga aagagccgtt ctttcagtaa aaggtaccat tagtggaatt gatgatattt
      481 cgagcataga aaagctttat ctttcctttt atctatcgaa ggagaatgca gagatagtaa
      541 agaatgagtt tgagcaagct cttagcatga gttttatgag cgatggtgaa aattacatta
      601 tttttgacca ggacagaaat atttttctat ccttaaagat tggagggggt atcggacagc
      661 ttaagtactg taaagatgct tctgactttt ttgagacaga aaaaattctt gaaaaagcca
      721 gtacctcaaa gctagaagac tttctgtact atgaagatga aaacagtaga gtatctttta
      781 taaaagtttt ttccgatgag tcctctttca agaaaatctg cctgtcagtt tcatatgatc
      841 atattataaa ctttctcaca gaaattatca tgagtgcttt tattaccaaa gacacagagg
      901 acgagattcc tgaaattgat tgggacagat atagcgagga tgataaaaat gactttggtt
      961 ggaagctttt tgatcttttt tccgcaataa atgttttaaa aaattctgac gaaacagtgg
     1021 ttctggatat ggaaagtctt tataataaag atcttttgaa ttatgagctt tatgaattaa
     1081 ctgacttttc ggttagaaaa tttatggatt atgaaggaca agaaacgttc gagatttttt
     1141 ataagggcaa tacggcagga tatataaatg attatgaaat atttgattat ctttattcgt
     1201 cttcggagta cttaactgtt gaaatagata gagataacct tacggtcttt gttagatgga
     1261 caaaataaaa cccgcttaaa agcgggtttt taatttttta gtttcttgct tgatccttca
     1321 tcagttgtct ggaggtaaga gaaagcaaaa ttatacctga tgccgccatt ataatattta
     1381 ttagccaaaa ggtagtgata ttcatgcttt caactcctgc aacatgccgt aaaagatctg
     1441 ctacatactt aatgggtaag atataactaa cccatcgaat atatccgggg ataatattaa
     1501 tgggaaagta tattcctgca aaaaacatca ttacagtcat taaaatagaa gcagcagact
     1561 gggctacttc caattcttta aacagtacca tcaaaagtat tccgaaagcc atcattccga
     1621 gtgtggagct taagcataca attataagca taggccagtc aaaggaatag ttcatgtgat
     1681 atattacttt gcccaatatt ataataatga ctatggaaaa aaagttcagc agtatcttgt
     1741 taaatgatga tgcaatcaca aattcgtttg attttattgg agttaccatg aattttttga
     1801 ttactcctaa tttcttatat ctgccgaagg tattgatcac actgaacatt cctccagaca
     1861 gtatggatat ggcaattatg cctgtaagaa gaaagtccat ttgattgctc tcaacccttc
     1921 ctgtaccaat attttcagaa gaaagagaca gataatcttt ttttgctccg tataaaaatc
     1981 tttttatagc gttctcacct gcaaacttta tccatgttat cttttgcata ttggcaggat
     2041 cggagttgta taaaactttt actttggagg tttcatcaac tataattcca gcatcaactt
     2101 ttgaagatag tacagcattt tttaagcttt cattatctgc atagatatca agcttatttg
     2161 gctcaaaaaa agcttttaca gtttcatatg cactctcgga gctctgattt gtatcagaca
     2221 agtaaccaga agaaaaaact aatccatatt cgaaattctc ttcttggtgc tgtgtatctc
     2281 ccaagccaaa aataaaaccg aacaaaagaa gaaagataac tggaaaaaac atggtgtaaa
     2341 aaacttcaga tttattttta aaagtttcaa gaaaaaagaa tttcacaaga ttgagaaatc
     2401 tattattcat agttcatcac cttcttgaag ttcaaagaaa aaattattat gcaggcgcca
     2461 atccaaagta agggaacaaa ataatttaaa aatcccagtg gcgtaccgtt tattatattt
     2521 cttaatcctt gagccatata tgataccgga ttaatataaa cgaaccatcg tataatcatc
     2581 ggagtatggc tgacatcaaa gaaaaggcct ccaaggaaca tcatcaactg ataaagaata
     2641 tttgccgcta cattgacggc cgaaagcttt ctaaaaaccg aaagcataag caatcctacg
     2701 gaaattaaag taataacgtt cataaaaaga taagcaatat acttcgcact gaatgcttta
     2761 ggactcactc ccattagttt gccttcaata tatatgagta agatagaaat acccaaaaga
     2821 ataagatttt ccaaaataac aacagttaag aaatggccgc tgcgtatagg ggttgtaaaa
     2881 atctttttgg taataccctt ttctatcgaa taagcaagtg aaagcggtgt attgaatata
     2941 gataccgtaa gaatcgacat taaaagaacg ctcgggaaaa gataatcttt ataggaaaaa
     3001 gtatccgagg cttttttatt taattcctga acctccagtg tatattctgc tgatgagatc
     3061 cgcttataaa actcgacatt aactgagtcc aaaacacttt taaaaatctt tctggcaaaa
     3121 tcagagccag aatccgatct gtaatagctc tttactaccg gagccggaac agaagaaagt
     3181 accactttga gattcagcat atcaagattt tccggaaaaa ctatgaaaat ttcaagtttt
     3241 ttatctttta taagctgtat accctcgtca aaggaactta cataaatgtt ttcgaaaggt
     3301 ccgccggagt tcttgtctcc cagaccggtt actaaacctt gtgaaatttc ctttgcaaga
     3361 ccagtaggcg gtttttcaaa gtaaattcct gctctaaact ccatatcgga cgagttttct
     3421 gatccgttac ctatattgcc gaaaagcgag gtaaagaaaa gaaaaaaaat caaaggaaaa
     3481 acaaaagtcc aaaaaattac tataaagttt ctgaaatcct cttttaaaaa gattgaaagc
     3541 aaagacttta tcttcatatg tcctcctaat ctcttaaggt tcttccggta agtttcaaaa
     3601 acacatcttc aagattaggc tgccttatag ctatttctga tattgaaaga ctgttttttc
     3661 ttgtggtttc aaatatattt gaaagagttg tctcaacatt atcagtctgt atctcatata
     3721 tctttttttc tgaggaaaga gctgtgtcct caattatttt aaggttctcg tccttgaaat
     3781 aactatcgaa ttttttatta gaatgaaatc ttataatgga tttcattcct attgaatcaa
     3841 tcaactgctc aactgtccca cacgcgatta cttttccgtg gtcaactata tttacaaaat
     3901 ctgaaagata ctcagcctct tccatgtaat gagtagtaag tataattgtt tttccgcttg
     3961 acttcagttc atttataaag tcccatagca ttctccttgc ctggggatca agaccggttg
     4021 taggttcatc aagaaaaaca atatcaggat catttacaag agccacagct attgcaagtc
     4081 tctgtttttg tccgccagat aaggttttta ttttggaatt gagtttttct tcaagttcta
     4141 gtttttttac aagaatatca atatcaaatt ttttttcata caaacctgaa aaacccttta
     4201 gagtttcctt tactgtcaaa gattcaaaaa aaacagtgtt ttgaagctga acacctattt
     4261 tttccttcaa ttcgaaattt acttttgtga tttcttttcc aaagtaggtt atccttccag
     4321 aatccgccct tctaagccct tctagaatct caactgtggt agtttttcct gcaccgttag
     4381 gacccagcag agcaaaaact tttccagctg ctattgagaa agaaatgtca tctacagcct
     4441 tcactgtttt gtagtacttc ctgagatttg atacttcaat tatgttttcc attttccctc
     4501 ctactgtatg aaaagtaaat ttgtccagcg aatagataaa aatataaaaa caaataataa
     4561 gatttctaaa aattataaca cataaaatat aaaaaaatta tttttctgca tgaaatgaag
     4621 ctatagtggt atggctagaa aaaaacgaag cgaaaaatcg ctccgtcaga tattaaagaa
     4681 aaattttttt aatacttaga tcatacggtg ttcgcaatac tcctttttcc gttataatac
     4741 cacttatatt tgcatgggga gtaacatcaa aggaaggatt gaaaaccttt acactgtcag
     4801 gaaccatttt aagtttatga caatagcgta cttcattatg atctctttct tctatgggaa
     4861 tgtctttacc ggattctgat tttatatcta tggttgaaac cggagcagca atataaaaag
     4921 gtatagagtg ctgtttagca agaatagaaa cagagtatgt accgatctta tttgctgtat
     4981 cgccgttggc agcaattctg tcggcaccga caattacagc gtttatcaat cctttatgca
     5041 ttacccagcc tgccatatta tcagaaatta aggtaacatc tatatggtct ttcatcaatt
     5101 cccatactgt aagtctcgcg ccctgaagat atggtcgtgt ttcatctgca tagactttaa
     5161 tctttttgcc tgatttaact gcggctctta ttacaccgag tgcggttcca aaatcaaagg
     5221 ttgcaagtgc cccggcgtta cagtgagtta atacggtatc gccatcctta agaagctgcg
     5281 caccatactc acctagagct tgattgactt ttatatcttc ctgagctata gcaatagctt
     5341 catcttccaa gatttttaca gtttcatatg tatcgttttc aagaataacg gtcttcattt
     5401 tcccttccat acgttcaagc gcccagaaaa ggtttacagc ggtaggtctt gttgcagcta
     5461 acgcattttt tacttctgcc atttttaata taaaactgtc tttggttttc ttttcaaact
     5521 cctttgctcc cagaacatat ccaaaagctg cacttgcacc tatcgcagga gcacctctta
     5581 ctatcatgtc ttttatcgct gtggctacct gagtataagt agtacactgc acataaactt
     5641 cctccaaagg aagctttctc tgatcaataa atattaagtt aaagtcagac cattccatgg
     5701 acattccttt tattctagac ataataacct ccggctttat ttttctgttt ttccgataat
     5761 ctttttggcg aggttgataa aaggtgccac tgtgtcagga ataccaaaat aagctgcagg
     5821 ctggccttta tccatattgc ttcgtataat tttatttatg ggtatttcct gaagaagctc
     5881 aagattatac ttttcagcca gagtttttcc gccgtcttgt ccgaatatat aatgtttttc
     5941 tccacatttt tcacatacaa agtatgacat attttctaca agaccaagga tatttacatt
     6001 catcatttta gagaaattaa tggctttttc cacatcatcc tgtgaaacta cggagggtgt
     6061 tgttactata agagtgccgt caatatcttt gaagttctgg aaaacagtca gaagctcatc
     6121 gcctgtaccc ggagggcagt ccattataag ataatcaagc tctccccaag ctatatcacc
     6181 aagaaattca agtattgcag atgtttttag aggtcctctc caaacaatag gcgaagtctc
     6241 gtccttaagg aactgggaag ttgatattac cttaattcca tttatttcgg gcggtaggac
     6301 atcgtttcca acctgataag gttttgcact gttgcctcca agcattctta caacatctgg
     6361 tccgtggaga tctatatcaa gaagtcccac ttttcttcct tcaagagcca atgcaaccgc
     6421 aaggtttaca gcaacagtag atttgccgac accacccttt ccgctcatta ccacaatctt
     6481 atgagagatg tttttcatgg tattctgagc tttttcaatc atagcattta ccggattttc
     6541 catctttatt tcctcctcag attatttctg tgtttccaca agttttattt catagcttag
     6601 ctttattgat cctttaagca ttgcagcaac cgtacagtac ttatcttgtg aaagctgcac
     6661 tgattgctgt atttttccca acggaagatc atgaccgctg aactcatata ttaggtgtat
     6721 tttttcaaaa tgtctcattt cgtcatcagt tctttcagta atcatacgta ttttaagaga
     6781 atcaaaagaa acccgttttt tctcaagtat catagtgaca tcgattccgg tacatccagc
     6841 gagacccgaa agaaccattt caacaggagt agctgcccgg ccatttcctc cagactctat
     6901 agtagcatca gtaaaaagat catgccctga aggagttctg ctgtaaaact ctctttttcc
     6961 agcatattca tacagatatt cttttgtcat ggatacatcc tcctttatac gtttaattat
     7021 attatacctt aaaaagttta aataagagta tctctggaga tatctggatt ttgaggattt
     7081 ataaaaatat cgaacaggac aagattatat ccgatattct caagagcttg attcaaagat
     7141 acgctgtttg actgcatata cttagtattt ctgtctattc ctaactttat tataacatca
     7201 tttttactca tgaatagatt tatatcagcc atgccaaagg tttcagtagg cagcaccatt
     7261 cttagttttc cataattctt ttctacagaa ttcttttcat ttccaaagtc atctgaaagc
     7321 cttctaaaat taaaaaaaac aggtattccc aaaaaactaa caagaaaata agtgcttgct
     7381 tcagttgttt ttgtgttttc aaacattttg taaacagaaa cagctttttc aactgcagta
     7441 gaaatggaat ttttagaaaa aagaccattg tcttcaaaag acaacttttc ggaaactttt
     7501 ttttcagcag atatttctgc tagtagttcg tttctgaaag agtccgatat ttttacttta
     7561 cccaaaacag tagcatggcg cgaatcattt tttagttctg tattttggtc tgtttgatgg
     7621 gaagtttcta tgattttttt ctgtggtaca ttaaaagaat tttttacagg aatttcttct
     7681 atcatatctg gctgttttct actttctgtt gttaaagaag atgctttttc aaaaatagtt
     7741 tttgaaacat tatgtgatag tttttcagat ataggttttt gctgagagct ctgctcagaa
     7801 ttatttactt caaaagtgct ttcttttcgg aaaaaattag tgattgcatc gtttttttgt
     7861 ttgctgatat aaactgttcg gatattattc cctgaatcaa agatatcacg taaaacagac
     7921 ttataaagct taaaagtagc aagatctatt ttttgagaaa ggttttctgc aggttctgtt
     7981 ttctttaacg atagatcggt ggcactcttt tcagtaagta cttcagaaat tacctttgct
     8041 ttgtgaaaag cagccggctc agaatttttc ttttttgcgg ctgaataatg atttttaaaa
     8101 taatcaagga tgagttcctt ttttaactga ttagaattcc tttgaataag cgattcatca
     8161 aatggttctt ttttaaaagt attttttgca gactgaaaat ctaaacttct tttctgaaaa
     8221 tgattctccg cttctttagg tatatctgat ttataggtat tacgagcaat ctgtggagaa
     8281 tcttgagatt tgattttttc atttttcgta aaaggtaaat catctttttg agataataaa
     8341 gtttttacct gattttctat tttatatgtc tgtttcttat caggtgcatg atcttttaaa
     8401 gaatcaagtt ttttttcatg agaactctcc tgaagaggtt cttttttaat taaaaagttt
     8461 ttatcaacca ttttatcttc ttgttcaaaa ttcgactgaa taagcggtga ttcattcttg
     8521 tcaatatcgg agctgttaaa aaggttggtt ggttttatat cttcagactg ttttgaactt
     8581 aagtatgaat ccttaaatag cgtaacagct ttttcagata tgaatcttgg atcggctgta
     8641 aagatcttgt tagaaaaaaa gtccttagaa agctcagata tcatagattt tatgaagccg
     8701 gaaaaaactt ctttcctgga aaaatcctct aaaacagaag aaaaagcttt gtcattttgc
     8761 attgaagtat ttagtgaatc aaatagatct gatatccaaa caagaaattt tcctttctga
     8821 gcatcaggaa tctcagaaaa atcaattttt atattcgata aaatactttg gttagtttct
     8881 tgggtaagac ctatggaaaa tttaattata tttccggaaa tttctcctaa ggcttttgtt
     8941 atatagcttt taataatatt ctgatcagtt gaaggtatag tattttttga aaaaacaata
     9001 tttaccaaag agccttcagg aagctttttt ccatcttcag aattaagtat atacagtttt
     9061 ccgttaatac ttacaagatt tatctctccg tcagagtata ttacctttcc tgtaatcttg
     9121 ccgtttttcg cagccagttc tgtcagctga gaagcatttg aagattgagt caggaatgta
     9181 tttagtatat tcataaaccc ctccttgttt ttcttaacat atcacatatc gtctgataaa
     9241 acatattaga ccagatattt tatcggttta tcttcaatag attaaaaaca cacaatactt
     9301 gaacgacatt aattgtatca tacatgatat aattaaatat gccgatttaa agggaggaaa
     9361 atatgggaat atttaaaaaa attttcagaa taactgatca tgaaaataaa aaagaatctc
     9421 aagtatttta cagtgaagga aactcgcttg tcgtatattt tcgttgtaaa aagtgcggag
     9481 aaaaatttgc tgcatatctt agaaagggat atgatctaat tactaattat gatgattctc
     9541 aagcaagtta tttcatagac aaggaattta taggttcaaa atgttttgag aaaataaatc
     9601 tttcagcaga gtttgatgaa agatacaaaa taataaagct taatcttaca ggtgccgata
     9661 ttcttacacg tcaggagtac gagaaagatg actgataatt ttgatactga taggagtatt
     9721 gatgtttttg agtaccgtcc ggtaagacct tttattcaaa taccggaaaa ggttatggtt
     9781 tatgttcttg gacttacccc gtcttcgttt tcgaaagtat atttatatat atataattta
     9841 agctatggct tagggcgtga ctatacacag gcctttacaa gcgtaagaga gatttcttcc
     9901 aatctaaata taagtatagg aagaacccag aatgcaatgg catatttcaa aaaaataaac
     9961 gcttttgtga agattgtttc caaggccgtt attggaagca agtacaaagt gaatgtcccg
    10021 gagtactcag cacaaagtgg aagctggtac tttgtagaac cagtcaaagt gaaatacctt
    10081 ctcctgaagg agaaacttat tacttcagat tcaattgtag agtctaagat caagcgttat
    10141 aaatcaataa gagagtctct taaaataggg ctttttgata accctgaaga ataaaaaaag
    10201 cccggtgagc tgttgctctc cgggtataat caaacatctt catggggggt tcgggggatt
    10261 actgactaaa ttatagctta taaagtttaa aaaaaaacta atattaagta acgaaataac
    10321 tttgttaatg aacgtgaata tataagaggc tttccacagg agagttttta aaatacgctg
    10381 atgttgcata aatttgactg gacatgtatt ctaatagcag tctgagaggt ggatatgtgg
    10441 ataattccag tataaaaata agtaggggtc atccgaaact tggtgctaca gttgatccat
    10501 ccggtacaaa cttcgctgtt ttttccagaa atgggaaaag agtttttata gaactttatg
    10561 agaattttta cgatgatatt cctttttttt cgtatgaact agaccgcatt tataataaaa
    10621 ccggagacat atggcatatt tgtctttttg ggataggtaa taacttatat tatctttgga
    10681 gagtagatgg ggattatgaa cctttgagtg gtcatagatt caataaaaat aaggttttac
    10741 ttgatccata tgctaaagct atatcgggtg tgtatgattt tgatgatccg tccatgtata
    10801 gttacgatac ttcagatgat tcaaacatgg atttaagttt ttcaaggact aactcagtac
    10861 gttcagcgtg taaaaatatt
//
LOCUS       DAOSHR010000059        10831 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_5115_length_10831_cov_5.730512, whole genome shotgun sequence.
ACCESSION   DAOSHR010000059 DAOSHR010000000
VERSION     DAOSHR010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 10831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10831
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5115_length_10831_cov_5.730512"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="PLU28_04650"
     CDS             <1..197
                     /locus_tag="PLU28_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34388.1"
                     /translation="PYSNVFLSDLQKAEVVFYQANSAKIQKHIEEAVQSVMFGNKTPE
                     EALKILKQKAQESLDEGRDM"
     gene            272..1195
                     /locus_tag="PLU28_04655"
     CDS             272..1195
                     /locus_tag="PLU28_04655"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOG34389.1"
                     /translation="MHTIKEMKHTGGIERKKAKWGWIFVVPSIVFFAIFSFYPIINAA
                     YTSFFKKNLLSLRPPKFIGFENYTYIFTSEDFWNSFRATITFTLGTFVPIVVFSLIFA
                     TFIMSRRRFQKFFQMLFYSPAVLSSVTAAVIWLMIFDLRGLANQLLDFLTNSPGIDRH
                     WLSSSAMVQLSTIIVYFWKYVGYFTIIFISGLASIPQSIHEASRIDGANSWQDFVHIT
                     LPLLKPTTVLVSIMSMLQCLKTFSTQYLFTQSGAPTQPINVITLNIYNTAIRDHSIGR
                     ASAMSIVLFLIMLLLTWFQFKVSKTDEVDYN"
     gene            1208..2062
                     /locus_tag="PLU28_04660"
     CDS             1208..2062
                     /locus_tag="PLU28_04660"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOG34390.1"
                     /translation="MEKYKRNFKSNLADTLIWIFLGVFAIIIIAPIIFMFCASFMTSN
                     EILKMPFSWIPKSFYYQNYIHAFSGNDGSYIYIRNILNSFIVASVVTVTTILLSAITG
                     YGLAKFKFKGRNFVFMLIMATMMIPFEAIMVPLYLVITKLGLQDSYTGLILPFLTNAF
                     GVFMMRQYLITFPDELLDAARIDGASELKIFWKIIMPNSLPVVATLGILTFRSEWDNM
                     LWPLLVVQSEEMKTIPLYIVKFTAEKHTNEGAMMAVAAIASIPMIILFFALSKYFVSG
                     ASLHASRK"
     gene            2144..2692
                     /locus_tag="PLU28_04665"
     CDS             2144..2692
                     /locus_tag="PLU28_04665"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOG34391.1"
                     /translation="MVRNCNVVPHIAQVYGTTEEELRNSEFMPMTDRLMSGEITETEF
                     WSWFNNKKTTSFKGLLWGETFSPSIITQTVEIINRLKKKYRVVCGTNTMQPHYDLHMK
                     NNDYGCFDKVYASHLMHAIKPDRDFYTYILSHEGVSASDAVFIDDMFVNVLGARTIGV
                     HGILFQTPQKLETDITMLIGSF"
     gene            complement(2726..3190)
                     /locus_tag="PLU28_04670"
     CDS             complement(2726..3190)
                     /locus_tag="PLU28_04670"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOG34392.1"
                     /translation="MPHLTLEEVNMDNLFKVCKLSDTLSPGQQKCVAPNVYSIAQAHF
                     YEHAWFRAIYLDGVPIGFIMVDTQPSDLPTEEVPSVFLWRFMIGRDFQHKGYGRQVLD
                     IITDKYRKEGKKYLYVSCEMDEEEGPYQFYLNYGFKDTGVMDDDEEVLKMKL"
     gene            complement(3212..4024)
                     /locus_tag="PLU28_04675"
     CDS             complement(3212..4024)
                     /locus_tag="PLU28_04675"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOG34393.1"
                     /translation="MSYKEINGIKYFYELRGNTESKETVAFFNGVMASADSWKNQVHL
                     FEKMGYKILLHDFKGQLRSDKPKGPYTFSQHAHEAKLLMESLGIEKVHIVGTSYGGEV
                     ALRFALDFPQAVKTISVIDSVSELDETLKFFVLGWKELAKQKEGEKFFFGIMPSIYGN
                     SFLKKNMEMLKSRAKAFKEISDSYFDGQIELYNTFINDVYITDQLSRIDCPVLVVCGE
                     DDILKPPKFSRLIAESVKNSEYVIIPQCGHVAIFEKPNELNSILLGFISKHL"
     gene            4282..5382
                     /locus_tag="PLU28_04680"
     CDS             4282..5382
                     /locus_tag="PLU28_04680"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HOG34394.1"
                     /translation="MDTIQINPTQHEILKSVWLKEHFARQELSEALNIDKSTVSRNLE
                     SLISQGLVLEAGEKNPGESGGRKTQILNFNKDYFNILGIAVVNGRALYTINDMQGNVL
                     FKDSIRFEKKDGEFFRGLDSVIELALKNYGNILAICISMPGIIDSAKGYVVFSEDLEL
                     KNLNLKKYVENKYKIYTFIENDANSGVASYLSHFRLKYSNLIYFMFFFPDKIFKFGGL
                     GAGIVIDKKIYRGSNSAAGEIKFEQELWFDKEKLYSIKDINTGFRENPEIMDVLNRIT
                     DMLSNRIYYLTNFLDPDHIILGGDINQFSEEVKNYLMSKIFRKFGITENNRFILVDAQ
                     GLVSIAAGGTISFMNAFMSDFAMAKKILKFGL"
     gene            5414..6628
                     /locus_tag="PLU28_04685"
     CDS             5414..6628
                     /locus_tag="PLU28_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HOG34395.1"
                     /translation="MLKRLLVLLLLVLTVSLGLAATKLTFWQFMMDQPTSDAVLEQFR
                     KENPDIQLEVVQLSWGNGKDKITTAFAAGNPPDVIELGNTWMPQFSSEGVLMNITSEI
                     KKYPNITGWYQTEYKNQYYGFPWLLGTRVLYYNVDLFIKAGLDPVNPPKTWEETLAAA
                     KKISALGKNIYGFGLCSGELYSPTQQWFLPAVWGNGGNVLNSDYTKATLNTKEVSDAA
                     KFYQELARYSIKDKQTGVEQAFGEGRVGMYVAGAWSISTLQTTYPKLNFYLGFVPKPS
                     KDKGTHASFAGGEILTIAKNTKNKDAAMKLVEYLLRDDVAMLITKRVPQIYPSNRLAI
                     LDPWWNENPLYQMFVKQNTYAVAAPLYTRWEDVEIELTSMIEEIILDYNDVDKTLKKY
                     NDKIQGILDRYK"
     gene            6683..7561
                     /locus_tag="PLU28_04690"
     CDS             6683..7561
                     /locus_tag="PLU28_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOG34396.1"
                     /translation="MKPKTKKRVTVLIFLLPWIITFFVFELYPLLYSLFVSFTKYSGL
                     NPQTTFIGLENYARAFQDEVFIKSVLNTLIFVAGTLPFTIAIAILIAILLNHLNLKFN
                     GLFKAGFFLPSMISMVVISMIWIYMYSGTGFFNTVLHFFGFAVEERSWLANQNTALGS
                     IMIMDVWASFGYYMILIYAGLQNIPYSVYEAARIDGAKPIQIAFKITIPMIRPTLFFV
                     IALNTIRSFQIFTEVFTMTNGGPRNSTETIVHYLYRVSFRNYEMGYGSAIAYILLAII
                     MTITLIQKRALRSDYL"
     gene            7558..>8810
                     /locus_tag="PLU28_04695"
     CDS             7558..>8810
                     /locus_tag="PLU28_04695"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOG34397.1"
                     /translation="MKTKKTFIAKLNIFILTVLLILSLIPLALTVITSIVPEGDLFNI
                     TKEVELSDFELPPFFLTKKIFPIESEKFDTVDGDNNSGKVLQLDTKDCVTGIMVNIGD
                     VDLNKIKEFTFSLNTSQKIKTFYLGFKDVQGMQEFLPAELTYVSGKWNNAIVHVNADK
                     YVVVDKEHLNFVGLKFEFENNEKILIDNVTLRYKFPTLWNYKNVLQQNDFARYMLNSA
                     IVSACHVFGNLIFCSMVAYVFARKKFRGKEVLFAIVLSSMMIPLQVKIIPIFKLMQFL
                     GWVDTLYALIAPGVVTSFGIFFMRQYIEQIPFELDQSAYVDGANDFKIFTKIIMPLST
                     PALAVLAINTFVASWNDLYMPLILTSSNNTRTVQVGLATFNRMHQVAWPELMAASSVV
                     GIPIIIIFLIFQKKIISGMVEGAVK"
     assembly_gap    8811..8910
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8911..10007
                     /locus_tag="PLU28_04700"
     CDS             <8911..10007
                     /locus_tag="PLU28_04700"
                     /inference="COORDINATES: protein motif:HMM:NF019193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-N-acetylglucosaminidase domain-containing
                     protein"
                     /protein_id="HOG34398.1"
                     /translation="DEDLEKLFEKYTFFSNLGVKKFSLFFDDIPLSLNHMADKEKYSS
                     LAHAQSDFSNRLYDMLLKKDQDAKLIICPTLYHGTDPVQYHTLLGECLDLRIDIMWTG
                     EDVCSQKLLTENTKKISHAFRRKVLYWDNFPVNDSLMCGELHIGPYESREKDLYLYSK
                     GIVLNPMNQAYASMLVMADAINYMNFPDSYDPVKSKELSLKELYPDAYNGLLTLVHFN
                     LKSPLHPQGFEKIKELALAFESEYSLRPVQAVEKYTILIEKHIQNLTSLEKTDEKLWK
                     DILPWVEELGAKLRIMLETFELIKLSSDIQCEFPSKTNIQKVEEKVFYLERKISESIG
                     FQTFSGGIDFLGFCLKYLLQAKSLLKLTQY"
     gene            complement(10091..10642)
                     /locus_tag="PLU28_04705"
     CDS             complement(10091..10642)
                     /locus_tag="PLU28_04705"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOG34399.1"
                     /translation="MNPDETVIELGAGYGRIIKEIAPFCKSVIGIDISENNVMLAKEY
                     LKNLPNADVIKMNVHSIELKSTFDIVLCLQNGISSMGITPEDIKNIMNLLNFGGHAFF
                     STYSSKFWDCRLEWFEEQAAKGLLGEIDTENTGDGVIVCKDGFRATTKSPEEFEKIGR
                     MTGYPFKIVEVDDSSLFLVISKE"
BASE COUNT         3365 a         2209 c         1943 g         3214 t
ORIGIN      
        1 atccttactc taacgtattc ctgtctgacc tgcagaaggc tgaggtcgtt ttctaccagg
       61 ccaactcagc caaaatacag aaacatatag aagaagccgt acagtcggta atgtttggaa
      121 acaagacacc tgaagaagct ttgaagatac ttaaacaaaa agcccaggaa tctcttgatg
      181 aaggaagaga catgtaaaaa aaacagttgc atatgggcca cttttaaagt ggtccatatc
      241 tttaaattaa ctttcgggga gcgtgtcgat aatgcacaca ataaaagaaa tgaagcatac
      301 aggaggcata gagcggaaaa aagcaaagtg gggctggatt tttgtcgttc cgtctatagt
      361 attttttgca attttcagct tttatcccat tataaatgcg gcatacacaa gttttttcaa
      421 aaaaaatctt ttgtcgctga gacctcctaa gtttataggg tttgaaaatt atacctacat
      481 attcacatcg gaggatttct ggaactcctt cagagcaacc atcacattca ccctggggac
      541 ctttgtccca atcgttgtat tcagtcttat atttgctaca tttataatgt cccgcagaag
      601 atttcagaag ttttttcaaa tgctttttta ctcacctgcc gttctttctt cggtcacagc
      661 ggcggtaata tggcttatga tattcgactt gcgcggactt gcaaaccagc ttttggactt
      721 tcttaccaac tctcccggaa tagacaggca ctggctgtca tcatctgcta tggtacagct
      781 ttcaaccatt atagtttact tctggaagta tgtgggatac tttaccataa tattcatatc
      841 aggactggca agcatacctc agagtattca tgaagcgagc agaatagatg gggcaaactc
      901 atggcaggac tttgtccaca taaccttacc gctgcttaag ccaacgaccg ttctggtctc
      961 aataatgtct atgctccaat gtcttaaaac tttcagcact caataccttt tcactcaatc
     1021 aggcgctccg actcaaccca taaacgtcat tactctgaat atttataaca ccgctataag
     1081 ggatcactca ataggaagag cctcggcaat gagcattgta ctatttttaa ttatgcttct
     1141 gcttacatgg ttccagttta aagtatcaaa aaccgatgaa gtggattata attaaagggg
     1201 gcatgacatg gaaaaataca aacgcaactt caaatctaat cttgctgaca cccttatctg
     1261 gatctttctg ggcgtctttg caataataat aattgctcct attatattta tgttctgcgc
     1321 atcatttatg acttccaatg aaatcctgaa gatgccattt tcatggatac caaaaagctt
     1381 ttactatcag aactacatac atgctttttc cggtaacgac ggaagctata tctatatacg
     1441 caacatactt aactccttca tcgttgcttc agtagttact gtaaccacta tactgctttc
     1501 agccataacc ggatacggac tggcaaagtt caagttcaag ggaaggaact ttgtttttat
     1561 gcttattatg gcaacaatga tgataccttt tgaagctata atggtaccgt tatacctggt
     1621 aataaccaag cttggcctgc aagactcata taccggactt atactgccgt ttctcacaaa
     1681 tgcctttgga gtatttatga tgcgccagta tcttataact tttccggatg aacttttgga
     1741 tgcagcaaga attgacggtg caagcgaact taaaatcttc tggaagatta ttatgcccaa
     1801 cagcctgccg gttgtcgcaa ctctcggaat attgaccttc cgtagtgaat gggataacat
     1861 gctttggccg ctgctggtag tccaaagtga agagatgaag accatcccgc tttacatcgt
     1921 caagttcaca gccgaaaaac ataccaacga aggtgcaatg atggcagttg ccgcaattgc
     1981 aagcatacct atgattatac ttttctttgc tttatcaaaa tattttgtaa gcggcgcaag
     2041 tttacatgct tcaagaaagt agtcagactt cttgacggta actgcacagg cttttaagga
     2101 gaggattaaa ctgaaacttt ttatttttga catgggcggt gtcgtggtaa ggaattgcaa
     2161 cgtagttccc catatagctc aagtttacgg aacaaccgaa gaagaattgc gaaacagtga
     2221 atttatgcct atgacagatc ggcttatgag cggagaaata acagaaacag agttttggag
     2281 ctggttcaat aacaaaaaaa ctacttcttt caaaggactt ctttggggag aaacctttag
     2341 tccgtccata ataacacaga ctgttgaaat aattaaccga ctaaagaaga agtaccgtgt
     2401 agtatgcggt accaacacta tgcagccgca ctacgatctg catatgaaaa acaatgatta
     2461 tgggtgcttt gacaaagttt atgcttcaca tctgatgcat gctataaaac ctgacaggga
     2521 tttttatact tatatccttt cacatgaagg agtttccgct tcagatgcag tttttattga
     2581 cgatatgttt gtcaatgtac tcggagcaag aacaatcgga gtacatggaa ttcttttcca
     2641 gacaccacag aagcttgaaa cagatataac tatgctgata ggcagttttt aaatgaaact
     2701 cccgcattaa tgcgggagtt ttctactata acttcatttt caaaacttct tcatcatcat
     2761 ccatgactcc ggtgtcctta aaaccatagt tcaagtaaaa ctgataaggc ccttcttcct
     2821 cgtccatctc acatgaaaca tagagatact ttttcccttc tttcctgtac ttgtcggtaa
     2881 taatgtcaag aacctgtctt ccatagcctt tatgctgaaa atccctgcca atcatgaacc
     2941 tccacaaaaa gacacttgga acttcttctg tcggaagatc cgaaggctga gtatcaacca
     3001 ttataaaccc tataggaact ccatccagat agatggctct aaaccacgca tgctcataaa
     3061 aatgcgcctg ggctatagaa taaacgttcg gtgcaacaca cttctgctgt cctggcgata
     3121 aagtatcgct gagcttacag actttaaaaa gattatccat attcacttct tccagagtta
     3181 gatgcggcac tgtacacctc ctgccataag atcaaagatg cttggaaata aaccccagaa
     3241 gaattgaatt aagttcattg ggtttttcaa aaatggccac atgaccacac tgcggtataa
     3301 taacgtactc tgaatttttt acagactcgg caatcagcct ggaaaatttc ggcggcttga
     3361 gtatatcgtc ttctccacac accacaagca caggacagtc tattctggaa agctgatcag
     3421 taatatacac atcgtttata aaagtattgt aaagctcaat ctgaccgtca aaataactgt
     3481 ctgatatttc cttaaacgcc ttagccctgc ttttaagcat ttccatattc tttttcaaaa
     3541 aagagtttcc ataaatactt ggcataattc caaagaaaaa cttctcccct tctttttgtt
     3601 ttgcaagctc tttccatccc agaacaaaaa atttaagcgt ttcatccagt tcacttacag
     3661 aatctattac cgatatagtt tttactgcct gcggaaagtc cagtgcaaaa cgcaaagcta
     3721 cttctcctcc ataagaggtt cctacaatat gaactttttc tattccaaga ctttccataa
     3781 gaagtttcgc ctcatgcgca tgctgagaaa atgtataagg tcccttgggc ttatccgatc
     3841 ttaactgtcc cttgaagtca tgaagcaaaa tcttgtaacc catcttttca aaaagatgaa
     3901 cctgattttt ccagctgtct gccgacgcca taaccccatt aaaaaaagct acagtttctt
     3961 tactttcagt attccctcta agttcataaa aatactttat accgttgatc tctttatacg
     4021 acaaatcaat gccccctcaa ttttaaagta atgatctttc ttttctttat aataatacca
     4081 tattattgac tgtacgcaga aaaaatatgc ttttttatta tctttaaata gaaacattca
     4141 aaatttttat ctatttcggt ttattttgcc ttttttgttt tttataacct caaatcagat
     4201 ttcttttaat agaggaaacc atgtaatatc atgttagttg catgatgcac gcaactaaca
     4261 ctttgaaaca ggaggtctac tatggacaca attcagataa atcctacaca acatgagatt
     4321 ctcaagtctg tatggctgaa ggagcacttt gcaaggcagg agctcagcga agctcttaat
     4381 atagacaagt ctacagtttc acgcaacctt gaatcgctta tttctcaggg attagttttg
     4441 gaggcaggcg aaaaaaatcc cggcgagtcc ggaggaagga aaacccagat acttaacttc
     4501 aataaagatt atttcaatat tcttggaata gctgttgtaa acggaagagc tctttatacc
     4561 ataaacgata tgcagggaaa tgtattgttt aaagacagta tcagatttga aaaaaaagac
     4621 ggcgagtttt tcaggggact cgattcagtt atagaactgg cgctgaagaa ctacggaaat
     4681 attcttgcca tatgtatatc aatgcccgga ataatcgatt ctgccaaagg gtatgtggtg
     4741 ttctctgaag atttagagct taagaatctg aacttaaaaa agtatgttga gaacaagtat
     4801 aaaatctata cttttataga aaatgacgct aactcaggag tagcctctta tctttcacac
     4861 tttcgcctta agtactccaa tctgatatac tttatgtttt tctttccgga caagatcttc
     4921 aagttcggag gactgggagc aggaattgtt attgacaaaa aaatataccg cggcagcaac
     4981 tccgcagccg gtgaaataaa gtttgaacag gagctctggt ttgataaaga aaagctctac
     5041 tctataaaag atataaacac aggcttcagg gagaaccctg aaattatgga cgttctcaac
     5101 agaattaccg atatgctttc aaacaggatt tactatctga caaactttct tgatccggat
     5161 catataattc tgggcggaga tatcaaccag ttttctgaag aagtaaaaaa ctatctgatg
     5221 tcaaaaatat tccgtaagtt cggaataacc gaaaacaaca ggttcatcct agtggatgca
     5281 caaggattgg tctctatagc agccggcgga actatatctt ttatgaatgc ttttatgagc
     5341 gattttgcaa tggcaaaaaa aatattgaag tttggtttat aaaagggata tttatactta
     5401 ggaggtggat taggtgttaa aaagactttt ggttctttta ttattggttc ttacggtaag
     5461 tctagggctt gcagcaacaa agcttacttt ctggcagttt atgatggatc agccgacatc
     5521 agatgccgtg cttgagcagt tcagaaagga aaatccggat attcagctgg aagttgtaca
     5581 gctatcgtgg ggaaacggca aggataaaat aaccactgca tttgctgccg gaaacccgcc
     5641 tgatgtaatt gaactgggaa acacatggat gccacagttc tcctcggaag gagttcttat
     5701 gaacatcaca tccgaaataa aaaagtaccc gaacatcacc ggatggtatc agaccgaata
     5761 taaaaaccag tactacggat ttccatggct tttaggcaca agagttcttt actacaacgt
     5821 agacctgttt ataaaagccg gattggatcc tgttaatcct cccaaaacat gggaagaaac
     5881 tctggcagcc gcaaaaaaaa taagtgcact tggaaagaat atttacggct ttggtctgtg
     5941 ctcgggcgag ctttactctc ctactcaaca gtggttcctt cccgcagtat ggggaaatgg
     6001 aggaaatgtt cttaattcag attataccaa agccacactt aatacaaaag aagtgtctga
     6061 tgccgcaaag ttctaccagg agctcgccag atactcaatc aaagataagc agacaggtgt
     6121 agaacaggcc tttggcgaag gacgtgtggg aatgtatgta gcgggtgcat ggtccataag
     6181 cactttgcag acaacatatc ctaaacttaa cttctatctt ggttttgttc caaagccttc
     6241 caaggacaag ggaacacatg catcttttgc aggcggagaa atattaacta tagctaaaaa
     6301 taccaaaaac aaggatgcag ccatgaaact cgtggagtac cttctcagag atgatgttgc
     6361 aatgcttata accaaaagag ttccgcagat atatccgtcc aacaggcttg ccatacttga
     6421 tccatggtgg aatgagaatc cactttatca gatgttcgta aagcaaaaca catatgccgt
     6481 ggcagctcct ctttatacac gatgggaaga tgtggagatc gagcttacaa gtatgataga
     6541 agagatcatc cttgattaca acgatgtaga caaaactctg aaaaagtaca acgataaaat
     6601 acaggggatt cttgacaggt acaaataact ttattgtcga atattcccgg tttatccggg
     6661 aatattcttt gggaggatta ccatgaagcc taaaactaaa aaaagagtta cggttcttat
     6721 atttctttta ccctggataa tcacattttt tgtttttgag ctctacccgc ttttatactc
     6781 tttatttgtt agttttacaa aatactcagg attaaatccg cagacaacct ttatcggcct
     6841 tgaaaactat gcaagggcat tccaagatga ggtttttata aaaagcgtac tgaatactct
     6901 catctttgta gcaggaacat tgccttttac aatagctatt gccatactca tagctatttt
     6961 gcttaatcat cttaacctca aattcaacgg acttttcaag gctggtttct ttttaccgtc
     7021 catgatctct atggttgtca tatcaatgat ttggatatac atgtactccg gaacaggttt
     7081 ttttaacact gtactgcact ttttcggatt tgcagttgaa gaaagaagct ggctggccaa
     7141 tcagaataca gccctaggct ctataatgat tatggatgtc tgggcatcgt ttggctatta
     7201 catgatcctg atatatgcag gactgcagaa tattccatac tctgtatatg aggctgcaag
     7261 aattgacggt gcaaagccta tacagattgc ctttaaaata accattccca tgataaggcc
     7321 aacacttttt tttgtcattg ctctcaatac aataagatcc ttccagatct ttaccgaagt
     7381 tttcactatg accaacggag gtccaagaaa ctctaccgaa actatagtac attaccttta
     7441 cagagtatcg ttccgtaact atgaaatggg ttacggttca gcaatagcat acattctgct
     7501 tgcaataatc atgacaatta ctcttataca aaaacgtgcc ctcaggagtg attacctgtg
     7561 aaaaccaaaa aaacctttat tgccaaactt aatattttta ttcttaccgt tcttttgatt
     7621 ctctcactca tacctcttgc tctgaccgta attacttcaa ttgttccgga aggagacctt
     7681 ttcaatatca ccaaagaggt tgaactgtct gattttgaac tgccgccttt cttccttaca
     7741 aaaaaaatct ttcctatcga atcagaaaag tttgatacag tcgatggaga caacaactca
     7801 ggaaaggttc tccaactgga tacaaaagac tgtgtcacag gaataatggt taatatagga
     7861 gatgttgatc ttaataagat aaaagaattc acttttagtc taaataccag ccagaagatc
     7921 aaaacctttt accttggttt taaagatgtc cagggcatgc aggagttttt gccggcagaa
     7981 ctaacatacg tttccggaaa atggaacaac gcaatagttc acgtaaatgc tgacaaatat
     8041 gttgttgtgg ataaagaaca tctaaacttt gtcggactga agtttgaatt tgaaaacaat
     8101 gaaaagattt taatcgataa cgttactcta agatacaagt ttcctactct gtggaactat
     8161 aaaaacgtcc ttcagcagaa cgatttcgca agatatatgc tcaactctgc aatagtatcg
     8221 gcctgccatg tatttggaaa tcttattttc tgttccatgg tcgcctacgt tttcgcacgg
     8281 aaaaaattcc gtggaaaaga agtactattc gccattgttc tgagctcgat gatgatacct
     8341 ctgcaggtaa aaatcatccc catattcaaa cttatgcagt tccttggctg ggttgacact
     8401 ctttacgctc ttatagcccc gggagttgta acttctttcg gtatattttt catgcggcag
     8461 tatatcgaac agataccctt tgaactggat cagtcggcat acgttgacgg agcaaacgac
     8521 tttaaaatat ttacaaaaat catcatgccc ctttcaacac ctgcccttgc cgtattagct
     8581 ataaacacct ttgtggcaag ttggaatgac ctgtatatgc ctcttatcct cacttcttca
     8641 aacaatacga gaactgtcca ggtaggactg gcaaccttca acagaatgca ccaggtcgca
     8701 tggcccgaac ttatggctgc ctcctctgtg gtggggatac cgatcattat aatattttta
     8761 atctttcaga aaaaaataat ctcaggcatg gtggaaggag ccgttaaata nnnnnnnnnn
     8821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn atgatgaaga tctggaaaag ctttttgaaa
     8941 aatatacttt cttttccaat ctgggagtaa aaaaattttc tctgtttttc gatgacattc
     9001 ctctttctct gaaccatatg gcagacaagg agaagtacag ttctcttgcc catgctcagt
     9061 ctgatttctc aaacagactg tacgatatgc tgcttaaaaa agaccaggat gcaaagttaa
     9121 tcatatgccc gaccctttat catggaaccg atcccgtcca atatcatacc ctcctcggag
     9181 aatgtcttga tctgcgaata gatattatgt ggacaggaga agatgtttgt tcccaaaaac
     9241 tgctgacaga aaatacaaaa aaaatatccc acgctttcag gagaaaagta ctctactggg
     9301 acaacttccc tgtaaatgac tctctaatgt gcggagaact tcatataggg ccttacgaat
     9361 caagggaaaa agatctttac ctctactcaa agggaatagt actcaaccct atgaatcaag
     9421 cttatgcaag tatgcttgtc atggccgatg ccataaatta tatgaacttt cccgattcct
     9481 acgaccctgt aaaatcaaaa gaactttcac tcaaagagct ttacccggat gcctataacg
     9541 gactcctgac gttagtacac tttaatctta aaagtccgct acatccgcaa ggatttgaaa
     9601 aaataaaaga gcttgcactg gcatttgaaa gcgaatacag cctcagacca gttcaagcgg
     9661 ttgaaaagta tacaattctt atagaaaaac atatccaaaa cttaacttcc cttgaaaaaa
     9721 cagatgaaaa actctggaaa gatatattac catgggtaga agaactgggc gcaaagcttc
     9781 gaattatgct tgaaactttt gaattgataa aacttagctc tgacatacag tgtgagtttc
     9841 cttcaaaaac caatatacaa aaagtggaag aaaaagtatt ctacttggaa agaaaaatat
     9901 cagaaagcat cggatttcaa accttttccg gcggcataga ctttttgggt ttttgcctga
     9961 agtatctgct ccaggctaaa agtcttttaa aacttaccca atactaactt atacactcct
    10021 cgtactctta acgttaaaat gcgcaggtga gctgatcacc tgctttttta tgttaaaaac
    10081 ccaaaatact ttactcttta ctgataacaa ggaaaagact ggaatcatca acttctacaa
    10141 tcttaaatgg ataaccggtc atccttccga tcttttcaaa ctcttcggga gattttgtag
    10201 tagctctaaa gccatctttg caaacaataa cgccatcacc agtattttct gtatcaatct
    10261 ctcccagtaa tcccttagca gcttgttctt caaaccattc aagacggcag tcccaaaatt
    10321 tagagctata cgtactgaaa aaggcatgtc ctccaaagtt tagcaaattc attatatttt
    10381 ttatatcttc tggtgttatt cccattgaag aaattccgtt ctgtaagcat aacacgatat
    10441 caaatgtact ttttagttca atactatgca cattcatttt tataacatct gcattcggaa
    10501 gattttttaa atactctttc gcaagcatga cattgttttc agatatatca attccaataa
    10561 cagatttaca aaaaggtgca atctctttga tgattcgtcc ataacccgca ccaagctcaa
    10621 ttactgtttc atctgggttt atagaactgc ggacataatc aatctctgcc tataaatatt
    10681 gttttatgcg cataattttt gtgtcatata ccttaaatag cttctctgca ttgagcttat
    10741 cgttatatta attatctccc acttttactt ccccttttta ctgcgttttt gccgatgcaa
    10801 ttgaattacc ttggcataat agatatatcc t
//
LOCUS       DAOSHR010000060        10645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_5230_length_10645_cov_5.562512, whole genome shotgun sequence.
ACCESSION   DAOSHR010000060 DAOSHR010000000
VERSION     DAOSHR010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 10645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10645
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5230_length_10645_cov_5.562512"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1081
                     /gene="hutI"
                     /locus_tag="PLU28_04710"
     CDS             <1..1081
                     /gene="hutI"
                     /locus_tag="PLU28_04710"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HOG34400.1"
                     /translation="DTQIKVIDAHGCSVLPGFVDSHTHFIFGGYRQDEFMDRLQGVPY
                     MEIMQRGGGIINSVKATRKATHEELASTGKKRLASMLSFGVTTVEGKSGYGLDLETEL
                     KQLRVMKELNTAQPVDIVSTYMGAHAVAPEYEKDPDRYIDFIISDVLPKVKEDKLAEF
                     CDVFCEKNVFSIEQSEKLLNSAKKLGMGIKAHADEIVPFGGAAMAARVGATSADHLLK
                     ASLQDITLMSQNNVVATLLPLTAFSLNEEYADARLMIERDCAVALATDMNPGSCFSES
                     IPLLISLAAIHMKMSIEEIIIALTINGAAAVGREKNIGSIDIGKEGDILILDSPSYKF
                     LAYHIGVSSVQTVIKNGKVVFSKGG"
     gene            1083..1700
                     /locus_tag="PLU28_04715"
     CDS             1083..1700
                     /locus_tag="PLU28_04715"
                     /inference="COORDINATES: protein motif:HMM:NF016825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HOG34401.1"
                     /translation="MLIDKKVSEFIDEVASDSSVPGGGSVAALSGAISAALVSMVSNL
                     TIGKKGYEDKEEHVKGILVKSEKIRQDLTLLIDEDSKAFEKVMKAFKLPKDTQEQKEL
                     RTSTIQQETKNASLIPLKAAKSAAEIFELARYVLLNGNKNAASDAAVSVMLARTAVLS
                     ACYNVLINLSSIKDQEFVQNIKQEVVQLQKNALETETEILSLYTF"
     gene            1716..2222
                     /locus_tag="PLU28_04720"
     CDS             1716..2222
                     /locus_tag="PLU28_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HOG34402.1"
                     /translation="MDFKEVSVKDISESVFRLFDDEWTIIASGNREKNNFMTASWGGM
                     GVLWNKEVCFIFIRPQRYTYEFVEKNNFFTLNFFDQTYRGVLEFCGTKSGREYDKLKE
                     TSLSPAFDDPGYMYFQQARLVFMCKKLYFQDISPNGFIDFDIDKTIYSKKDYHRMYVG
                     AIERVISK"
     gene            complement(2241..3464)
                     /gene="pepT"
                     /locus_tag="PLU28_04725"
     CDS             complement(2241..3464)
                     /gene="pepT"
                     /locus_tag="PLU28_04725"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HOG34403.1"
                     /translation="MENLVKRFLEYVKIYTTSDEDSTTFPSTQRQKDLAVFLKKELEQ
                     LGLAEISLDHNGYLMATLPANTEEKIPVIGLIAHMDTSPEVSGENVKPMIIKYGGGDI
                     VLNVEQNIVLKTLNFPEIEKYKGQTLITTDGTTLLGADDKAGIAEIITAVEYLINNPH
                     IKHGKVRIGFTPDEEVGRGTEKFDIKAFGADFAYTVDGGPLGELEYENFNGASAEYIL
                     KGKSVHPGTSKDKMVNANLMVADLLALFPLAETPQNTEKYEGFYHVTKITGTVEQARV
                     HFIIRDHDRQEFEYKKQICMRNAMIMKNKYGQDRVEVDIRDSYYNMKQIIQENMFIVD
                     YAVKAFKQCGIEPHIVPIRGGTDGARLSYMGLPCPNIFTGGHNFHGRYEYIPVSSMEK
                     AVQAIVEIIKVIAEK"
     gene            complement(3474..4781)
                     /locus_tag="PLU28_04730"
     CDS             complement(3474..4781)
                     /locus_tag="PLU28_04730"
                     /inference="COORDINATES: protein motif:HMM:NF015380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HOG34404.1"
                     /translation="MRKKKFILISLIVVLVFMISGCVIFPSELSSVYLPKGNYDKALF
                     FRTDVSNVEFNGLPLEKKEGYYVLAYEKAAGLDYGTVRYESSNGQMHTEKVFFRDTSF
                     QMVIYSGGDNSLTSWTDHIWEDLSEICDAVANSSLNMTVNFIADYKSKPDRLWTVYKF
                     EGSYACIERLIETYNSGEAEIDSGSCAAAESIILEVASENKNTDLFLDFWNHGSGWLD
                     ESYVKSMKSIVQDSTSGTFLKIGDVRSILSSLKQKRGRNTDIVGFDACNMSCLEIIYE
                     VSELADYFVGSAFLEDVQGWHYEFLKEYIGNNEELLKALVSSYAKYYKGYKNISMSAV
                     RLRGFKSFIKESLCSIKSRQELLPVYYLGSFEAVMTDVIQSIPQIKEFLVSGYVYTDK
                     TENYSGIGFAYSIGSLIAKQDYKQLLFYKDNSSWIESVWGSLK"
     gene            complement(4778..5479)
                     /locus_tag="PLU28_04735"
     CDS             complement(4778..5479)
                     /locus_tag="PLU28_04735"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOG34405.1"
                     /translation="MSKINVVKITVNKLKTVFVLKNENSSILIDTCHRNQRLQFYSIL
                     ENLEIEIAKIKLIVITHAHTDNYGVASEIKELYGIPVLVQRQDANRMIRGVSADYKPV
                     GIRGRIFSKYFYRESCCPKAIKVEPDIIVDDSYDLSDFGISATVFHTPGHTCGSLSVL
                     TKDGDLILSDQFQVKKGIPSRITAPLICYDLKQSIFSLKKMSDYAPRKIYTASGYELS
                     YSELQRFIIKHEGTL"
     gene            complement(5525..6181)
                     /locus_tag="PLU28_04740"
     CDS             complement(5525..6181)
                     /locus_tag="PLU28_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34406.1"
                     /translation="MLKFYLQQEILSKAPNWEYNCQTGYYEYARGGENDYSIKFLGDF
                     SIKVVCCDDLYILIAPDGKFLQMPSILDCEAVGEILLTLENIKKYNSMLRKEYRKKIN
                     FYKTNNRLKNTRKLSRLYREVKVYSDENFEKQDFNENLYVLYDRNSSISFEVERSEYQ
                     LCLKIPQEKFRENTLISDEIFFCEEQDLQYFINAMKDLRKFRRQNFFFSKNNSLVSRI
                     "
     gene            complement(6269..8986)
                     /locus_tag="PLU28_04745"
     CDS             complement(6269..8986)
                     /locus_tag="PLU28_04745"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HOG34407.1"
                     /translation="MRYKNLFFLFFLLLTVSVCFSQKKNILVLFSYHPELGWNQEIFS
                     ELEKELNTSKYPVDIFYEYMDTKNIISKEYFAKLAEVYAMKYAKKVIDVIVCVDNDAL
                     DFLLEYRDVLFGEKPVVFLGINEFYPEMLKGKKGFTGVVESIDFKKTIDIALKLTPQI
                     KRFLVYGDNSSIYYMNKSQIEKESISFPEINFIIRNDMSIEQVIQDVRQAGPQSALII
                     ISTLRSETGELVNYEDIAPTISTYSDIPIYVPWEFLIKGNVVGGYVISGYDLAYEAAQ
                     MVLLILQGKDVGEIPVLTNTLNRYIFDYNMLVKYSVDMGSIPENAVLISKPRRFIDEY
                     RDVFLIFLVIFAAACILIVIILFYGFKKRQLSIKLENEKKIKESIIESASIMIIAIDE
                     QRRIQIFNKYAQKVSGYDKTEVLGQKVYKFLDPDFPGSVLEGTLLCKDSGVKTIVWKK
                     SGVYDRTTGEKLELFTGDDITQKKKSEAALYYSANFDQLTGLANRTLFLKVLSEQIEK
                     SPDEKVSIFLMDIDRFKFVNDVHGHVFGDEVLKEVSKRLKKHFEKDGAFARMGGDEFI
                     GYIRNRTSIQDIKDISEQILKSFSEPLKLNDADFFISISMGVTTYPDGGNDCGELLKN
                     AEISMYKSKESGGNAFEVYETRMKRKISEKLFLQSHLRRAINEKQFLVYYQPIVDISG
                     WKIKEAEALVRWNHPQWGIVPPSKFIDIAEESGFISAIGEEVLHNVCKDIIKTKQELS
                     IEIKISLNLSMQQLCDPYLIEKFDHITEGYGINKELISVEVTESMAMQKLPVVRNTLY
                     EFKKRGMGIMLDDFGTGYSSLSYLTLLPIDFIKIDRSFVNKISATDRDRKVISTIVAM
                     AKGLNVQIIAEGVENEEQLDFLKKIGCDTIQGYYFSKPLSYIEFIKMFQ"
     gene            complement(8983..>10645)
                     /locus_tag="PLU28_04750"
     CDS             complement(8983..>10645)
                     /locus_tag="PLU28_04750"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG34408.1"
                     /translation="LMSCIFCLLNSYILAKYLDKGKFEKKLLQLFFILLFFFNLSFVF
                     WVDGFSLWSGIIVLFCGLVNFAVGIVMFIVFMNKVISANAGSSENFIKLFTQSSIPFL
                     IVKGKNNEIYDANNAAEIFLGVGREILKTMTLGDAENEYRQRTKNQDDLFEDCISVWY
                     NDQRMEVVSLKNQSFFHPDYSGISSQQDHPVVNEDTIEKSVQLEQDRQVSMKAESILT
                     MADLLGRTLNDGEQSEYIVRIEESAREILACKNEMYRQACEKTKNDRQIKTRFNLKNL
                     LDGAYRQFAENAENKDISTSIEFLEVEQDSIYEGDPVKLRLLLSCVLEKLLFYTESNE
                     LSIKAQKIYHDRENSYFVFSITEGRYIQSPANDEADHRTNVRDCFSECLDLASQAGGK
                     IWTEKTDGLRKYEITFVLGNTFPVSQAESLSVESFQDKLNILVVEDNEINQKLICAIL
                     KKRGWNPLVADNGLKALEVLKDKSFDIVLMDVQMPYMDGYETTRRIRQSENQTVAKIP
                     IVAMTAYAMTEHRQSCIKSGMNDYITKPVNMSDLYTIIERNLSKR"
BASE COUNT         3198 a         2254 c         1823 g         3370 t
ORIGIN      
        1 tgatacgcag ataaaagtta ttgatgctca cggctgctct gtgcttccgg gctttgttga
       61 ctcacacaca cactttatat tcggcggata cagacaggat gagtttatgg accgtctgca
      121 gggagtaccg tacatggaga ttatgcaaag aggcggaggt atcataaact ctgtaaaagc
      181 cacgcgtaaa gcaacacatg aagagctggc ctccaccgga aaaaagagac tggcctcaat
      241 gctttccttc ggagtaacta ccgttgaggg aaaaagcgga tatggtcttg atctggaaac
      301 agagttgaaa cagctgcgtg taatgaaaga actaaacact gcccagcctg tcgacatagt
      361 tagtacttat atgggtgcac atgcagttgc acctgagtat gaaaaggatc cggacagata
      421 tatagacttt atcatatccg acgttctgcc caaagtaaaa gaagataaac tggcagagtt
      481 ctgtgatgtt ttctgtgaaa aaaatgtctt cagcatagaa cagtcagaaa aacttctcaa
      541 cagcgcaaaa aaacttggaa tgggaatcaa agcacatgca gacgaaatag tcccctttgg
      601 cggagcagca atggctgcaa gagtcggagc aacctctgca gatcatcttc ttaaagcaag
      661 tttgcaggat ataactctta tgtctcaaaa caatgtagtg gcaacactgc ttccccttac
      721 tgccttcagt cttaatgaag agtatgcgga tgcaagactt atgattgaaa gagactgtgc
      781 ggttgctctt gcaacagata tgaatcccgg ctcctgtttc agcgagtcta taccgttgct
      841 tatctctttg gctgccatac atatgaagat gtccatagaa gagattatca ttgcccttac
      901 cataaacggc gctgctgctg tgggacgcga gaaaaacatc ggctctattg atataggcaa
      961 agaaggagat attcttattc ttgattctcc ttcatataag ttccttgctt accatatagg
     1021 cgtaagctct gttcagacag ttataaaaaa cggaaaagta gttttttcaa aaggaggata
     1081 aaatgcttat tgataaaaaa gtttctgagt ttattgatga agttgcttca gactcttccg
     1141 ttccaggagg aggaagtgtc gctgcactta gcggagccat cagcgctgct ctcgtttcaa
     1201 tggtttccaa cctcactata ggaaaaaaag gctatgaaga taaagaagaa catgtgaaag
     1261 gaattctggt taagtccgaa aaaatacgtc aagatctaac cttacttata gacgaggaca
     1321 gcaaggcctt tgaaaaggtc atgaaagcct ttaagcttcc aaaagacacc caagaacaga
     1381 aagaattaag aaccagcaca attcagcagg aaaccaaaaa tgcatcactt ataccgctta
     1441 aagcagccaa aagtgcagct gaaatatttg agcttgcaag atatgttctg ctgaacggaa
     1501 ataaaaatgc agcttccgat gccgcggtat ctgtcatgct tgcgcggaca gcggttctgt
     1561 cagcctgcta taatgtgctt ataaatcttt cctccataaa agatcaggag tttgttcaaa
     1621 atataaaaca ggaagttgta cagcttcaaa aaaatgcttt ggagactgaa acggagatac
     1681 tttcattata tactttctga aaagaaggtg atagtatgga ctttaaagaa gttagcgtaa
     1741 aggatatttc cgaaagcgtt ttcaggcttt tcgacgatga atggaccatc attgcctcag
     1801 gaaacaggga gaagaacaac tttatgaccg cgagctgggg cggtatgggc gtactttgga
     1861 acaaggaagt ctgttttatt ttcataagac cgcagagata tacttatgag tttgtggaaa
     1921 aaaacaactt ttttactctg aacttctttg accaaaccta caggggagtt ttggagttct
     1981 gcggcaccaa atcaggaaga gagtacgata agcttaaaga aacctctctt tcacctgctt
     2041 ttgatgatcc aggatatatg tactttcaac aggccagatt agttttcatg tgcaaaaagc
     2101 tgtactttca ggatatttct ccaaatggtt ttattgactt tgatatcgat aagaccattt
     2161 attcaaaaaa agactatcac agaatgtatg taggagctat agaaagagta atatcaaaat
     2221 aaaacccgct catgcgggtt ttatttttca gcaatcacct ttattatttc aacaatagcc
     2281 tgaacagcct tttccattga agaaaccgga atatactcgt atcttccgtg gaagttatga
     2341 cctccggtaa atatattcgg acatggcagc cccatataag aaagcctggc tccgtctgtt
     2401 ccgcctctta taggaactat atgaggctca attccgcact gcttgaaagc ttttaccgca
     2461 taatccacaa taaacatgtt ttcctggatt atctgtttca tgttatagta agaatcacgt
     2521 atatcgacct caactctgtc ctgcccatat ttatttttca ttatcatagc atttctcatg
     2581 catatctgtt ttttatactc aaactcctgc ctgtcatggt ctcttattat aaaatgtact
     2641 ctggcctgct caactgttcc ggttatcttg gtaacatgat aaaagccctc atatttttcc
     2701 gtattctgag gagtttctgc aagaggaaac aaagcaagaa gatcggccac catgagatta
     2761 gcgtttacca tcttgtcttt tgacgttcca ggatgaacgc ttttgccttt aaggatatac
     2821 tccgcactcg caccgttgaa attttcatac tccagctcgc ccaacggacc gccgtctaca
     2881 gtatatgcaa aatccgctcc aaatgctttt atatcaaact tctctgttcc tcttccgact
     2941 tcttcatcgg gagtaaaacc tattctgacc ttaccgtgct ttatatgagg attatttatt
     3001 agatactcaa cagccgttat tatttcggca atcccggctt tgtcatctgc tccaagcaaa
     3061 gtggttccgt ccgtggttat taatgtctgt cctttatact tttctatctc aggaaaattg
     3121 agtgtcttaa ggacaatatt ttgctctaca ttcaaaacaa tatctccgcc gccgtacttt
     3181 attatcatcg gcttgacatt ctctccgctt acctcaggtg aagtatccat atgggcaatt
     3241 aatccaatca caggtatctt ttcttcagta ttggcgggaa gtgtagccat aagatatcca
     3301 ttgtggtcaa gagaaatttc agcgagccct aactgttcaa gctctttttt gagaaaaacg
     3361 gccagatcct tctgcctttg ggtactggga aaagttgtag aatcttcgtc agaagtagta
     3421 taaatcttaa catattcaag aaatctcttt accaggtttt ccattttttc ctcctatttt
     3481 aaggatcccc atacactttc tatccatgat gaattatctt tataaaaaag aagctgctta
     3541 taatcctgct ttgcgatcaa agaacctata ctgtatgcaa aacctatacc gctgtaattc
     3601 tctgtcttat cagtatatac ataccctgaa accagaaatt cctttatctg cggtatactt
     3661 tgaatcacat cggtcatgac tgcttcaaaa gagcccagat agtatactgg caaaagctcc
     3721 tgcctggact tgattgaaca aagcgattct tttatgaatg acttaaaccc gcgaagtctt
     3781 acagcgctca tcgaaatatt tttatagcct ttataatatt tggcatacga agaaacaaga
     3841 gcttttaaaa gttcttcatt atttcctatg tactctttta gaaattcata gtgccatccc
     3901 tggacatctt caagaaaggc agagcctaca aaataatcgg ccaactcaga tacttcataa
     3961 attatttcaa gacatgacat attacaggca tcaaatccaa ctatatctgt atttcttcca
     4021 cgtttttgct taagtgatga taaaatgctt cttacatcgc ctattttaag aaaagttccg
     4081 gaagtactgt cctgaactat tgatttcata ctctttacat agctttcatc cagccagccg
     4141 cttccatgat tccaaaagtc taagaacaga tctgtgtttt tattttcaga ggccacttct
     4201 aaaattatgc tctcagcagc cgcacagcta ccagaatcaa tttccgcttc tccactgtta
     4261 tatgtctcaa tcagtctttc aatgcatgca taacttcctt caaacttata aacagtccac
     4321 agtctgtctg gtttgctctt ataatcggca atgaagttaa ccgtcatatt tagggaactg
     4381 tttgcaacag catcacatat ttcggacaga tcctcccaga tatgatctgt ccatgatgta
     4441 aggctgttat ctcctcctga gtatatgacc atctgaaacg atgtatcacg gaaaaaaacc
     4501 ttctcagtat gcatctgtcc gtttgaactt tcatatctga cagttccgta gtctaacccg
     4561 gcagcttttt cataggcaag cacataatat ccctcttttt tctcaagcgg taagccattg
     4621 aactctacat tacttacatc tgtacgaaaa aaaagagctt tatcataatt gccttttgga
     4681 agatataccg atgaaagctc agacgggaaa ataacacatc ctgagatcat aaataccaat
     4741 actacaatta aagatatcag aataaacttc tttttcctca taaagtaccc tcatgtttta
     4801 taataaaccg ctgcaattca ctataggaaa gctcatatcc gcttgctgtg tatatttttc
     4861 tgggagcgta atcggacatt tttttcaggg aaaagatgga ctgtttgagg tcataacata
     4921 tcaaaggcgc tgtaatcctg gacggaatgc cttttttcac ctgaaattga tcggaaagaa
     4981 taagatcgcc atccttggta aggacgctta aagatccgca agtatgacct ggagtatgaa
     5041 atactgtggc agaaatacca aaatcgctga gatcatatga atcatcaact ataatatccg
     5101 gttcaacttt aatcgctttg ggacaacagg actccctata gaaatacttg gaaaatatcc
     5161 ttcctcttat ccctacgggt ttataatcgg cggacactcc tcttatcatc ctgttggcat
     5221 cctgtctctg aaccaacaca ggaataccgt acagctcttt tatttcggaa gctacaccat
     5281 agttgtcggt atgggcatga gtaataacta taagtttgat tttggctatt tctatctcaa
     5341 gattctccaa gatgctataa aattggagtc tctgatttct atggcatgtg tctataagaa
     5401 tggaactatt ttcgttttta agcacaaaaa cagttttcag tttatttaca gtgattttca
     5461 ccacatttat ttttgacata cccgtctccg tttctaatta ttttaaaatt tacatgatcc
     5521 ttcctcatat tcggctgaca aggctattat tttttgaaaa aaaaaagttc tgcctgcgaa
     5581 actttctgag atccttcatc gcatttataa agtattgaag atcctgctcc tcacaaaaaa
     5641 agatctcatc gcttataagc gtgttttctc tgaatttttc ttggggtatc ttaaggcaca
     5701 actgatattc cgaccgttcc acttcaaagc ttatactgct gttacggtca tacagcacat
     5761 aaaggttttc attaaagtcc tgtttttcaa agttttcatc cgagtatacc tttacttcac
     5821 ggtaaagacg gctgagcttt cttgtgtttt tgagcctgtt attggttttg taaaaattta
     5881 tctttttacg gtactccttc ctaagcatag aattatattt cttaatattc tccagtgtca
     5941 agagtatctc gcctactgct tcgcagtcaa gaatggaagg catttgtaag aacttgccat
     6001 caggagctat cagtatataa agatcatcac aacagactac ttttatactg aaatcgccta
     6061 aaaactttat ggaataatca ttttccccac cgcgcgcata ctcgtaatac ccggtttggc
     6121 agttgtattc ccagttcgga gccttactca gtatctcctg ctgcaaataa aatttaagca
     6181 taatttctcc cccgttttac attctactta aattctacca tataaagaca taaaaaatga
     6241 tatttttaaa tattaaataa ttacttcttt attgaaacat ttttatgaac tcgatataac
     6301 ttaacggttt gctgaaataa tagccctgga tggtatcaca gcctattttt ttaaggaagt
     6361 ctagctgttc ttcattttca acaccctcag cgataatctg gacattaagt ccttttgcca
     6421 tagctactat tgtacttata accttacggt ccctgtctgt agcacttatc ttgttcacaa
     6481 aacttctgtc tattttgatg aagtctatcg gcaggagcgt aagataactg agggaagagt
     6541 agcctgttcc aaaatcatcc agcattattc ccattccacg ttttttaaac tcataaaggg
     6601 tattacgcac aaccggaagc ttctgcatag ccatactttc ggttacttct accgaaataa
     6661 gctctttgtt gataccgtag ccttcggtta tgtgatcaaa cttttctatc agataaggat
     6721 cgcaaagctg ctgcatagaa agattaagac ttatctttat ttctatcgaa agctcctgct
     6781 tggtctttat tatgtctttg cagacattat gaagcacctc ttcgcctatg gcagatataa
     6841 agccagactc ctcggcgata tctataaact tggacggagg tactattccc cactgcggat
     6901 gattccacct tacaagtgct tctgcttcct ttatcttcca accggaaata tcaactatag
     6961 gttgatagta aactaaaaac tgtttctcat ttatagctct gcggagatga ctctgcagga
     7021 aaagtttttc ggatatcttt cttttcattc tggtctcata gacttcaaaa gcattaccgc
     7081 cggactcttt agacttatac atagatatct cggcattttt cagtagctct ccgcagtcgt
     7141 ttccgccatc cggatatgtt gtgacaccca tgcttatact tataaaaaag tctgcatcgt
     7201 taagcttaag aggctcagaa aagcttttta gtatctgctc ggaaatatct ttaatatctt
     7261 ggatcgaagt tctgtttctt atatagccaa tgaactcgtc gcctcccatt ctggcaaaag
     7321 ctccgtcctt ttcaaaatgc tttttcagcc tttttgaaac ttctttaaga acctcatctc
     7381 caaaaacatg tccgtgaaca tcatttacaa acttgaacct gtcaatatcc atgagaaaaa
     7441 tgcttacctt ttcatccgga cttttttcta tctgttcaga aaggactttt aaaaaaagag
     7501 ttctgttggc aagtcctgta agctgatcaa aatttgcact gtagtaaagc gcagcttcag
     7561 atttcttctt ttgggtaata tcgtctccgg taaaaagctc aagcttttca cccgtagttc
     7621 ggtcatagac accggatttt ttccatacta tcgtcttcac acccgaatct ttgcataaaa
     7681 gtgtaccctc cagaacagat ccgggaaaat caggatcaag aaacttatat actttttgcc
     7741 ctaatacttc ggttttatcg tatccgctta ccttttgggc atacttgtta aaaatctgta
     7801 ttctacgctg ctcatctata gctattatca ttatcgaagc actctctatt atgctttctt
     7861 ttattttttt ctcattttca agcttaatac ttaactgacg ctttttgaag ccataaaaaa
     7921 gaattattac tattaaaata catgctgcgg caaatataac caaaaagatc aaaaaaacgt
     7981 ccctgtactc atcaataaac cttctgggct tacttatcaa aacagcattt tcaggaatgc
     8041 ttcccatatc cacggaatat tttacaagca tattgtaatc aaaaatatat ctgttgagcg
     8101 tattggtcaa aacaggtatc tctcctacat ctttgccctg aaggatcaga agaaccatct
     8161 gggcagcctc atatgcaagg tcatagcctg atatgacata acctcccaca acatttccct
     8221 ttataaggaa ctcccatgga acgtatatag gaatatccga gtatgtagat attgtaggcg
     8281 cgatatcctc gtaattgaca agctctccgg tttcgctgcg cagagtagaa attataataa
     8341 gcgcactctg cggacctgcc tggcggacat cctgtattac ctgctctata ctcatatcat
     8401 tgcggataat aaagttgatt tcaggaaaag aaattgattc tttctctatc tgacttttat
     8461 tcatatagta gattgagctg ttgtctccat aaacaaggaa cctcttgatc tgcggtgtaa
     8521 gtttaagtgc aatatcaatg gttttcttaa aatcaatgct ctcaactact ccggtgaaac
     8581 ctttttttcc tttaagcatt tcaggataaa actcatttat tccaagaaaa acaaccggct
     8641 tttcgccaaa aagaacgtcc ctgtactcaa gaagaaagtc aagtgcatca ttatcaacgc
     8701 agacaataac atctataacc ttttttgcat atttcatagc gtatacttcg gcaagcttgg
     8761 caaaatactc tttgcttatt atattcttgg tatccatata ctcgtaaaaa atatcaacgg
     8821 gatacttcga agtattaagc tctttttcaa gctctgaaaa aatttcctga ttccagccaa
     8881 gctcaggatg atatgaaaaa aggacaagaa tatttttttt ctgagagaaa cataccgaaa
     8941 ctgttaataa aagaaagaat agaaaaaata agtttttata cctcatcttt ttgataaatt
     9001 cctttcgatt atagtataca gatcactcat gttgacaggt ttagttatat aatcgttcat
     9061 tccgcttttt atacagcttt gcctgtgctc cgtcatggca taggccgtca ttgccacaat
     9121 gggaatcttt gcaacggtct ggttttcaga ctgccttatt ctccgtgtgg tctcatatcc
     9181 atccatataa ggcatctgaa catccataag aacgatatca aaacttttgt ctttgagaac
     9241 ctccaaagct ttcagaccat tatccgccac caatgggttc catcctcttt ttttcaaaat
     9301 tgcacagata agtttctgat ttatctcatt atcctcaacc acaagtatat taagcttgtc
     9361 ttggaagctt tcgacagata agctttcagc ttgggatact ggaaaagtat tccccaaaac
     9421 aaaggttatt tcatactttc tcaaaccgtc ggttttctct gtccatatct tgccgcccgc
     9481 ctgagaagca agatccaaac attcggaaaa gcagtcccta acgttagtcc tatgatcagc
     9541 ttcatcattt gcaggagatt gaatataccg tccttcggtt attgaaaaaa caaagtacga
     9601 attttctctg tcatggtaga ttttttgtgc ttttatggaa agttcgttac tctcagtgta
     9661 aaaaagaagc ttttcaagaa cacaggacaa cagaagtctt aattttactg ggtcgccttc
     9721 ataaatactg tcctgttcca cttccagaaa ttcaatgctt gtacttatgt ctttattttc
     9781 agcattttca gcaaactgtc tgtaagctcc gtctaaaaga tttttaagat taaatctggt
     9841 tttgatctgc cggtcatttt tggttttttc acaagcttgc ctatacattt catttttaca
     9901 ggccagtatt tctcttgcac tttcttctat gcggactata tattctgact gttcaccatc
     9961 attaagtgta cgtcccagca gatccgccat cgtaaggatg gactccgctt tcattgatac
    10021 ttgcctgtct tgttcaagct gaactgactt ttcaatagta tcttcattta caacaggatg
    10081 atcttgctgt gaagaaatac cagaataatc tggatgaaaa aaactttgat ttttcaaaga
    10141 aacgacttcc attctctgat cgttgtacca aacacttata cagtcctcaa aaaggtcatc
    10201 ctgatttttt gttctctgcc tgtattcatt ttcagcatcc cctagagtca ttgtttttaa
    10261 gatctctctt cctacaccta aaaaaatttc agcagcatta ttagcatcat atatctcatt
    10321 gttctttcct tttacaataa gaaaaggaat actgctctgc gtgaaaagct ttataaagtt
    10381 ctctgaactc cctgcattgg cagaaatcac cttgttcata aatactatga acatcacaat
    10441 ccctactgca aagttcacca gtccgcaaaa cagaacaata atccctgacc ataaagaaaa
    10501 accatcaacc caaaaaacaa aggataagtt aaaaaagaaa agcaaaataa aaaaaagctg
    10561 aagaagtttt ttctcaaatt tacctttatc taagtatttt gcaagtatat aagaattcag
    10621 cagacagaat atacaggaca taaga
//
LOCUS       DAOSHR010000061        10580 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_5280_length_10580_cov_5.372922, whole genome shotgun sequence.
ACCESSION   DAOSHR010000061 DAOSHR010000000
VERSION     DAOSHR010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 10580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10580
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5280_length_10580_cov_5.372922"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="PLU28_04755"
     CDS             <1..634
                     /locus_tag="PLU28_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208905.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HOG34409.1"
                     /translation="KDIIRNMGLENPQIVYFKELAPGFTFFNGYGECTKTIDFTSIHV
                     AKADVKKMGMKDIDEYIKQTLNRKIVVVGASTGTDAHTVGIDAIMNMKGFAGHYGLER
                     YEMFEAYNLGSQVLNEDFIAKAIELKADALLVSQTVTQKNVHIKNMTELVEMLEAEGI
                     RSKVVLIAGGARITHELAKEIGFDAGFGPNSFAEDVGAFIAQEMARRLGK"
     gene            682..1269
                     /locus_tag="PLU28_04760"
     CDS             682..1269
                     /locus_tag="PLU28_04760"
                     /inference="COORDINATES: protein motif:HMM:NF003191.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HOG34410.1"
                     /translation="MNDLLSSDILKWVVMPVVIFIARILDVSLGTTRIIMVSKGKKAI
                     AAFIGFFEVTIWLLVASKVIQSVDNIVYVIAYAGGFAAGSYIGIVLEDKLAIGKIAVR
                     IITVKDPVEFIKSLRSMGYGVTSIQASGSTGQAHIIYSIMHRSKYKAFNEATNMFDPK
                     AFISVEDVKYSKDGIFHPDQHKLRLENLFPIRKSK"
     gene            1442..2158
                     /locus_tag="PLU28_04765"
     CDS             1442..2158
                     /locus_tag="PLU28_04765"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOG34411.1"
                     /translation="MADFELVCVDMFQTLVNLDTVEENIWKGVLGEYYTPQLKAACAY
                     SFKKKVYTRFHLFTSNAESFMTVREIVKPFFEEVRAEQNLNFDPDKASEIFIVQHGFS
                     KPYPDTLTFLSHLKNKIPVCLVSDADTLSIEPLLKIFSFDSAFFSEKYGSYKNDISNR
                     LFKEVIKFYGIAPEKIIHIGDSPSDIQGAKREKMTACWLNRNARQWNGSISPDHTVSD
                     LTGVLEILNIKKSVAVMKEK"
     gene            complement(2159..4150)
                     /locus_tag="PLU28_04770"
     CDS             complement(2159..4150)
                     /locus_tag="PLU28_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34412.1"
                     /translation="MKKIFLFLFILLGIMVQASTVVPYIFINSRLNIDYKSVYAILKE
                     KLSGYDVIERDKYYETFESTSKNTDNSYIIRLMKDNRSADYGVIVRINDCYTERKTEK
                     NVRFVKNPSGSYISYAGTFYQVSQKKLFSYNKNEDKFYQDSNGNYIYLADYPWARTER
                     EKFIKIDGFYTRYSEDTVWYKYSIKGSYSVIDYQSSVVIDSGSIDNSMVSKSSSEMQS
                     FVNSSIRAIKLRQSTFYEEKPKIALYALKNQEYSGYTDQIYGQVQQGFIDDGRYGILD
                     RANIDKIMDELKLDFLGIVSGQAASNMKIASYMVSFVINSLRYEEYTVTEKKSYLNPV
                     NGSYTAGEEVEVGKYYYRKRDGSFTPSATGTYVKKIRKPWERTDSYIPASDFYDVFTQ
                     DTVYAKGYMNCTFHVIDLKTGLYMGYMTKELSVNKPLTDLKDRFQSTESIFRSDVNDL
                     LIRQASSWINQETRKLFPLNTLIAASDRDRQIIQAGKNYGDKNSMLFRVVDDYITRGY
                     IKVEQTGEKTSNTRTIRLIKQYDEIKYNNLVYEDFDYYNPFGMQIYLGGGTGGFVTGL
                     GYMFTDIYKNLLGDIRAGMMIHGENSYFLGQAGIKVYTIQETDLSLALAFKTDFKHDI
                     SIYPVLEVSTRIFYDLLRYSAGFYIGKESLGFLNLSVCF"
     gene            complement(4163..5476)
                     /locus_tag="PLU28_04775"
     CDS             complement(4163..5476)
                     /locus_tag="PLU28_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34413.1"
                     /translation="MKKRVVLMILLLIAVTVLNARKSIKFFWSCSPQVPKTIRQNILN
                     DIKSSVISSERFLISNEEEIQEIAFEKKVESFFTSSDSIMSGVKDSDYIVAVKIFDYY
                     SSSKVERGSYMRDDLSGDYVFYDNAFIPVFKGYFYSYDAFAGRYIVDKNGDFVAGKNG
                     GYYPAGNGFYISSRYEDVFTDSGVSVQYILISAQDGKKIFEDTFSGRFSDYTVKYSYD
                     SQTGKVFIKQVPESAVWTAISSGYGDHVYSRLRDDFMIKAKIIDFSKDMVYIDAGANE
                     GIKKGYLFISQDASGKMIMKVENVNPQTAEGKILYMERGKELKISETVSEIKNFYDAW
                     ILGISFFAGTELGASLEFKNYDVYNEVTSCGGIDLILNQSKVQVSGVSFGLSVFKNEQ
                     INLLLRTGVYNADPFIGAHAKYLFFGILGDYSISGKFRAGITFSF"
     gene            complement(5545..7266)
                     /locus_tag="PLU28_04780"
     CDS             complement(5545..7266)
                     /locus_tag="PLU28_04780"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HOG34414.1"
                     /translation="MIGNMKIRTKLLLLLFIPLIGIVCIISLMAINFQNVVTDLELSL
                     KNENSKVISTVFEVDKSLSNTLSAFQNILSKGMSDPSFQSQYDEYKKGVDNAKKKLAV
                     AEEIIRNNKDGWLLYTNDVSQKTVEENFKNFYSNIDMWLEMTEVEIQAELTESFSSQY
                     FYDARANIAEIGQSADKFLAYKIQKNRERTNNLIFIAVIVGIGAFVIAFLIGFMIIID
                     ISSKVKKIMTVTQLGEGNLRIKFDIKGKDEFSNIGKTLNKVVESLRESMKNIETASDE
                     IRNSSKELGDVSQDTIKNSELLAGDAEKIQQNVENTSASIQEVTSSVEEVAAAAQSVS
                     KNSVDLSARTNDVAAAAEKGEKSIEAIVNVVETAVRSTDETASTVMKLTEHVGNIQQI
                     INTINSITEQTNLLALNAAIEAARAGDAGRGFAVVADEIRKLAEQSKQATVNIVSIIS
                     QIQERSRKADEITKKTVSDVRNVSSLAGEISLQFKDILGQVEKIHDMVQVMSANAQEQ
                     SAAAEEVSGTMDASARMVVNISEKIKDMVEAINKQAVSAQIISTSGEKMDGLSENLKS
                     LVSKFKV"
     gene            7617..>10580
                     /locus_tag="PLU28_04785"
     CDS             7617..>10580
                     /locus_tag="PLU28_04785"
                     /inference="COORDINATES: protein motif:HMM:NF037016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOG34415.1"
                     /translation="MKFRKYSLAFVLFFLLSFLLIGCSVQTNENKTVTKPEPIKINKA
                     PLISIPDQFIKEEDSLTLDLSKYSSDPEASPLTYSLVSGVGTIKSEAYFYSPKRGEFG
                     EKSIIISVTDGASSTQASFKISVEKVLKLPTVEFPDFSVEEGKSIEIDLSKYSSNPLK
                     DKLSYSIISGVGILNDFNYFFQPSYLDAGQKAVKIRIANYDGYIDQTFRLNIINVNRP
                     PTIFITNQTMDELQTLTLDLARIATDPDGDSLTFRLEEGPGSISNKVYSYSPTYKDQG
                     EKNVRISVSDGIARAETSFKVLVNNINRPPYMLVQEQEVDEGQTLLLDLGKIVFDPDG
                     DTLSFKIEEGVGEIVGSMYTFLPTYFDAGKKMVKLRVSDGTSTVYTTFSINVKNMNRV
                     PVIKVPDQSVDEGKTLLIDLSAHTYDPDNDKITYTLESSIGKLKGSIYEYSPDFFDAG
                     EKTVKIRANDGNSNIETLIKIVVNNVNRAPSIKVSDQQVNEGQTLIFDLSRYSDDPDT
                     DTLTYYVQEGVGSINGSQFIYTPGYFDAGTKKVTVRASDGSLSAVSSFNINVKNVNRA
                     PSMAVPDETVDEAQTLTLDLSKLSSDPDKDGLSYYSVNNVGSISGSIFTYTTTYDDAG
                     EKLARVGVSDGELSYETYFKIIVKNVNRAPQISIPDQTAFENNKLTLDLSKYSSDPDN
                     DKLSYSVLNQTGKIEGNSFIYTPSYFDAGEKVVQIAAFDGKLYGYSTFKITVNNVNRA
                     PSIFIPDQEIDEGKTLNINLFELSSDLDGDTLKYFIGNGIGSINGNNYTYSPAFSDAG
                     IKIVEVIAFDGIDRTAAKFKINVKKGNRTPIISIPNQTVNEGQTLALDLSKLSSDPDG
                     ERLTYSLENGVGEIKGRIYTYSPAYNEAGEKTVKIKVSDGISGVETTFKVYVSNVNKP
                     PVFSIPDIFIQEGKTVSIELKDYTKDPDGDSITFAFENGVGKVESGIFIYTPSYEEAG
                     QQHCQILISDGAVTVG"
BASE COUNT         3277 a         2126 c         1886 g         3291 t
ORIGIN      
        1 aaaggatata atcagaaaca tggggcttga gaaccctcag attgtatatt ttaaggaact
       61 ggcaccaggc ttcacttttt tcaacggata tggtgagtgt accaaaacta tagacttcac
      121 ttcaattcat gttgcaaaag cagatgtaaa aaagatgggt atgaaagaca tcgatgaata
      181 cataaaacaa actttaaaca gaaaaatcgt agtggtcggt gcaagtacag gcactgatgc
      241 gcacactgtc gggatagatg ctataatgaa tatgaaaggc tttgccggac attacggact
      301 tgaaagatat gagatgtttg aagcatataa ccttggaagt caggtattaa acgaagattt
      361 tattgcaaaa gcgatagagc ttaaagcaga cgcattactt gtatctcaaa ccgtaactca
      421 gaaaaacgta cacataaaaa acatgacaga actagttgaa atgcttgaag cagaaggtat
      481 aaggagcaag gtggttctta ttgccggagg tgctagaata actcacgaac tggccaaaga
      541 gataggattt gatgcaggct ttggacccaa ctcatttgca gaggatgtag gtgcgtttat
      601 agcccaggaa atggcaagac gtcttggcaa atgattttta tggtataatc tacttatctg
      661 aaatgattgg agggaagtgt tttgaatgat ctactgtcat cagatattct caaatgggta
      721 gttatgcctg ttgtgatctt cattgcaaga atccttgatg tatctctcgg aaccaccaga
      781 ataattatgg tttccaaagg aaagaaggcc attgcggctt ttataggctt ctttgaagtg
      841 accatatggc ttttggttgc cagcaaggtt atccagagtg tggacaacat agtttatgta
      901 atcgcttatg caggaggctt tgctgcagga agctatatag gcatagtcct ggaagataaa
      961 ctggcaatag gaaagatagc cgtacgcata ataaccgtca aagatcctgt tgagtttata
     1021 aaatcccttc ggtctatggg atacggggta acaagcatac aggcaagcgg ttccacaggg
     1081 caagctcata taatatacag tataatgcac cgttcaaaat ataaagcttt taacgaagcg
     1141 accaacatgt ttgatcctaa agcctttatt tcagttgaag acgtaaagta ttcaaaagac
     1201 ggtatttttc accctgacca gcataaactc aggctagaaa acctctttcc aataagaaaa
     1261 tccaaatgaa acaactttcc ggagagcttt actctccgtt tttttatttt taatattact
     1321 tttaaaagaa gctatgatct ttgtgaaaaa aagatagtat aattatactt agactatata
     1381 ctccaagcaa aagttttttg attagatttt actcatgttt tcaggaagtc cggaggtact
     1441 catggctgat tttgaactgg tatgcgtgga tatgtttcag acacttgtga atttagacac
     1501 cgttgaagaa aatatctgga agggagtcct tggagaatac tatacaccgc agttaaaagc
     1561 tgcatgtgcc tattccttca aaaagaaagt atacaccaga ttccatctgt ttacttcaaa
     1621 tgccgaatct ttcatgacag tcagggagat agtgaaacct ttttttgaag aagtacgtgc
     1681 tgaacaaaat ctaaacttcg atcctgacaa agcctctgaa atcttcatag tgcaacatgg
     1741 tttttcaaaa ccttatcctg ataccttaac ttttctgagc caccttaaaa acaagattcc
     1801 tgtatgtctt gttagtgatg ccgatactct ttccatagag ccgcttttga aaatattcag
     1861 ctttgacagc gctttctttt cagaaaaata cggatcgtat aagaacgata tttccaacag
     1921 gctttttaaa gaggttataa agttttacgg aatagctccg gaaaaaataa ttcacatagg
     1981 agactcaccg tctgacatcc aaggggctaa aagagaaaaa atgactgcct gctggcttaa
     2041 caggaatgcc cgtcagtgga acggaagtat aagccccgac catactgtaa gcgatctgac
     2101 cggggttttg gaaatattaa acattaaaaa atctgttgct gtcatgaaag aaaaataatc
     2161 agaaacagac acttagattt aaaaagccca agctttcttt accgatataa aacccggcac
     2221 tgtatctcaa aagatcgtaa aagatccggg tcgaaacttc caaaacagga tagatgctta
     2281 tatcgtgttt aaaatctgtt ttaaacgcca gagccagaga taggtcagtc tcttgaatcg
     2341 tgtacacttt tattccggcc tgtcccaaaa agtaactgtt ttctccatga atcatcattc
     2401 ctgctcttat atcccctaaa agatttttat atatatctgt gaacatatat cccagacctg
     2461 ttacaaaacc tcctgttcct cctcctaaat atatctgcat gccaaaagga ttatagtaat
     2521 caaagtcttc atatacaaga ttattatact ttatctcgtc atactgttta atgagtctta
     2581 tcgttctggt gttggatgtt ttttctcctg tctgctcaac ctttatatag cctctggtta
     2641 tataatcatc aacaactctg aataacattg aatttttgtc accataattc tttccagcct
     2701 gtattatctg tctatctcta tcactggcag ctataagcgt atttaaaggg aaaagctttc
     2761 tggtttcctg atttatccag ctacttgcct gtctgattaa aagatcgttt acatcggatc
     2821 tgaaaatact ttcagtgctc tgaaaacggt ctttcagatc tgtaagcggt ttattcacag
     2881 aaagttcctt tgtcatatag cccatgtaaa gccccgtttt aagatctatc acatggaatg
     2941 tacagttcat ataacctttt gcataaaccg tatcctgagt aaaaacatcg taaaaatctg
     3001 aagccggaat atatgagtcc gttctctccc aaggctttct gatttttttt acgtatgtgc
     3061 ctgtcgcact tggagtaaat gacccatccc tcttcctgta atagtacttc cccacttcaa
     3121 cctcttcgcc tgcggtataa ctaccgttga cagggttaag atagcttttt ttctcagtaa
     3181 ccgtgtactc ttcatacctt aaagaattta taacaaaaga aaccatatat gaggctattt
     3241 tcatgtttga agccgcttga ccgcttacta tacctagaaa atcaagcttg agctcatcca
     3301 ttatcttgtc tatatttgct ctgtcaagga tcccatacct tccgtcatct ataaaccctt
     3361 gctgaacctg tccatatatc tggtcggtgt atcctgagta ctcttgattt ttaagggcat
     3421 aaagagctat cttgggcttt tcttcataaa aagtgctctg tctaagtttt attgccctga
     3481 tgcttgagtt aacaaagctc tgcatttctg agctgctttt actgaccata ctgttgtcga
     3541 tactgccgct gtctattacc acggagcttt ggtagtcaat aacagaataa gaacctttta
     3601 tagagtactt ataccaaacg gtatcctcag aatatcttgt ataaaaaccg tcaatcttta
     3661 taaatttctc acgttcggtt cttgcccaag gataatctgc aagatatata taatttccgt
     3721 tgctgtcctg ataaaactta tcctcattct tgttgtagga aaaaagcttt ttctgactta
     3781 cctgatagaa cgtacctgcg tatgatatgt aagaaccaga aggatttttg acaaaccgta
     3841 cattcttctc agttttcctt tcggtatagc agtcgtttat gcgcactata actccataat
     3901 cggcagatct attatccttc atcaatctga taatatatga attatcagta tttttagacg
     3961 tgctttcaaa ggtttcatag tacttatcac gctcgatcac atcgtatccg gagagctttt
     4021 ctttaagtat ggcgtaaaca gatttatagt cgatattaag cctgctgttt ataaagatat
     4081 agggcacaac tgtcgatgcc tgaaccatta ttcctaaaag gataaaaaga aacagaaaaa
     4141 tttttttcat atatgccgcc ccctagaaag aaaaagtaat gccagcacgg aactttcctg
     4201 atatactata atctcccaaa ataccaaaaa agagatactt tgcatgtgct ccaataaacg
     4261 gatcagcgtt gtataccccc gttcttaaaa gaagatttat ctgttcattt ttaaatacag
     4321 acaacccgaa cgaaactccg gaaacctgta ccttgctttg attaagtata agatctattc
     4381 cgccacatga agtaacttca ttgtatacat catagttttt gaactctaaa gatgcaccca
     4441 gttcagtacc ggcaaaaaag cttattccta aaatccacgc atcataaaaa ttcttgattt
     4501 cagagacagt ctctgaaatc ttaagctctt tgcctctttc catataaagt atttttcctt
     4561 ccgctgtctg aggatttaca ttctctactt tcattatcat ctttcctgaa gcatcctgtg
     4621 aaataaaaag ataacccttt tttatgcctt cattagcacc ggcatctata tataccatat
     4681 cttttgaaaa gtcaattatt ttggctttta tcataaaatc atccctaagc cttgagtaga
     4741 catgatctcc gtatcctgaa gatatggctg tccataccgc actctcgggc acctgtttta
     4801 tgaagacctt tcccgtctgg ctgtcataac tgtacttgac ggtatagtcg gaaaatcttc
     4861 cggaaaacgt atcttcaaag atctttttcc cgtcctgggc actgattaga atatactgta
     4921 cgctaactcc gctgtcggta aaaacatcct catatctgct gcttatataa aaaccatttc
     4981 ctgcaggata gtacccgcca ttttttcccg caacaaaatc accgttctta tccacaatat
     5041 atcttcccgc aaatgcgtca tagctgtaaa agtagccctt aaagacagga ataaaagcat
     5101 tatcataaaa aacataatcg ccgctaaggt catcacgcat ataacttcct ctttcaactt
     5161 tactgctgct gtagtaatcg aatatcttca cggcaactat ataatctgag tctttgacac
     5221 cgctcattat actgtctgaa gaggtaaaga agctctcaac ctttttttca aaagctatct
     5281 cctgaatctc ttcctcgttt gaaatcagaa atctttccga agatattaca ctggacttta
     5341 tatcgttgag tatgttctgt cttattgtct taggcacctg tggactgcat gaccagaaga
     5401 attttatgct ttttcttgca ttaagtacag taactgctat taaaagaagt atcataagca
     5461 caactctttt tttcatgaaa ccacctccgc aggtattata ccaaaaaaag gctcccttac
     5521 ggagcctatc gtttgtatag cttattatac tttaaactta gatacaagag atttgagatt
     5581 ttcagaaaga ccgtccatct tttcccctga cgtacttatt atctgagcgg aaacagcctg
     5641 tttatttatg gcctcaacca tatcttttat cttttctgat atatttacaa ccattctggc
     5701 tgaagcatcc attgttcctg atacctcttc ggcggcagca ctctgctcct gagcattggc
     5761 cgacattacc tgaaccatat cgtgtatttt ttcaacctgt cctagtatgt ctttaaactg
     5821 tagacttatt tctccagcca aagagcttac attacgaaca tcagataccg tttttttggt
     5881 tatttcatca gcttttcttg atctttcctg aatctggctt atgatggaaa ctatgtttac
     5941 tgttgcctgc ttgctttgct cggcaagctt cctaatttca tcagcaacaa ccgcaaaacc
     6001 tcttcccgca tccccggctc ttgcagcttc tatagctgca ttgagagcta aaagattcgt
     6061 ctgttcggtt attgagttta tagtgtttat tatctgctgt atatttccca catgctctgt
     6121 tagcttcatt acggtactgg ctgtttcatc agtgctcctc acggcagttt caacaacatt
     6181 aactatagct tctatagatt tttcgccttt ttcagcagcg gcggcaacat cattggttct
     6241 ggccgaaaga tcaactgaat tttttgatac cgactgtgca gcagcggcaa cttcttctac
     6301 acttgaagta acttcctgta ttgaggctga tgtgttttca acattctgct gtattttttc
     6361 cgcatcaccc gcaagaagtt cggagttttt aatggtatcc tgcgatacat cgccaagctc
     6421 cttggaagag ttccttattt catcggatgc ggtttcgata tttttcatag actctcttag
     6481 agattctaca accttattaa gagttttacc tatgttagag aactcatcct ttccctttat
     6541 atcaaacttt atcctaagat ttccttcgcc tagctgagtt acagtcatta tttttttgac
     6601 cttagaggaa atatctatta ttatcatgaa acctatcaga aacgctatga caaaagctcc
     6661 gattcctaca attacagcta tgaaaataag gttatttgtt ctttctctgt tcttctgtat
     6721 tttatacgca aggaacttat cagcggattg acctatctct gctatattag ccctggcatc
     6781 gtagaagtac tgtgaagaga aagattcggt aagctctgcc tgaatttcaa cttcagtcat
     6841 ttccaaccac atatctatgt tgctgtaaaa gtttttaaag ttttcttcaa ctgttttctg
     6901 gcttacatcg ttagtatata aaagccaacc atctttattg tttcttatta tctcctcagc
     6961 aacagcaagc ttttttttgg cgttatcaac tcctttttta tactcgtcgt actgtgactg
     7021 aaagctcgga tctgacatac ctttggaaag aatattctga aatgcagaca aagtatttga
     7081 aagtgatttg tcaacctcaa atactgtcga tattaccttg gagttctcat tcttgagcga
     7141 cagttcaaga tctgtaacca cattttggaa gttaatcgcc ataagcgata tgatacatac
     7201 tattcctatc aacggtataa aaagaagaag taagagcttg gtacgtatct tcatattccc
     7261 aatcatagat tcccctccta aacattggaa ttagcctata gatttctttt tttgtcctat
     7321 actcaattat atcatcaacg atttagaaca cgtacaattg aaattgtttt ttaaatacat
     7381 cgacaactct ttataatcgt ggattatagc ttatgatttt tattagttac ttaaaataca
     7441 ttgaaattta ataatcaaaa cagcaaatgg ttcagtacct atatatatgt ctttacattt
     7501 aaaagttttt ttgatgtctc ataaacatga tactttttgt cataagaatt aaaaatgtag
     7561 tataattgaa tagaatcgac tttttcaaaa tacttaatat tttccggagg tataccatga
     7621 aatttagaaa atattcactg gcctttgttt tattcttttt actttcattt ttattaattg
     7681 gctgttctgt acagaccaat gaaaataaaa cggttacgaa accagaacca ataaaaataa
     7741 ataaagctcc gctcataagc attccggatc aatttataaa agaggaagat agtcttaccc
     7801 tggatctttc aaagtacagt tccgatcctg aagcttctcc tcttacttat tcccttgttt
     7861 cgggtgtggg tacaataaaa agtgaggcgt acttctattc tcccaagaga ggagagttcg
     7921 gagaaaaaag cattataata tctgttacag atggcgcatc atctacacaa gcatccttta
     7981 aaatttctgt agagaaggtt ttaaaacttc cgacagttga attcccggat ttttcggtag
     8041 aagaaggaaa aagtatagaa atcgatctga gcaagtattc ctctaatccc cttaaggata
     8101 agctttcata ttctatcata agcggtgtgg gaatattaaa tgattttaat tacttttttc
     8161 agccctcata tcttgatgcc ggtcagaaag ctgtaaaaat aagaatagcc aactatgatg
     8221 gttatataga ccagaccttc agacttaaca ttataaatgt aaacaggcct cctactatat
     8281 ttataaccaa tcagactatg gatgaacttc agaccctaac tcttgatctg gcaaggattg
     8341 ccactgatcc tgacggtgat tcgcttacct tcagactgga agaaggtccg ggcagtattt
     8401 caaataaagt gtactcttac tcgcctactt ataaagacca aggtgagaaa aacgtaagaa
     8461 tatcggtcag cgacggtata gcaagagccg aaacctcttt taaggtactt gtaaacaaca
     8521 taaatagacc tccttatatg cttgttcagg aacaggaagt cgacgaaggc cagactcttt
     8581 tgcttgatct gggaaagatt gtctttgatc cggatggaga tactctcagc ttcaagatag
     8641 aagagggtgt cggcgaaatt gtaggcagca tgtatacgtt tcttccaact tattttgatg
     8701 ccggtaaaaa gatggtgaag ctccgcgtaa gcgatggaac ttcgactgtt tataccacat
     8761 tcagcataaa cgtcaaaaac atgaatagag tccctgtcat aaaagtaccg gatcaaagcg
     8821 tggatgaagg aaaaaccctt ttgatcgacc tatccgcaca tacttatgat cctgacaacg
     8881 ataagataac ttatactctt gaaagcagta taggaaagtt aaagggcagc atttacgagt
     8941 atagcccaga cttttttgat gccggagaaa aaacggttaa aataagggca aatgacggta
     9001 attctaatat tgaaactctt ataaagatcg tggtaaacaa tgtgaacaga gctccttcga
     9061 taaaggtttc agaccagcag gtaaatgaag gacaaactct tatatttgac ctttcaagat
     9121 attcagacga tcccgatacg gatactctta cctactatgt acaagaagga gtcggaagta
     9181 taaacggcag ccagttcata tacactcccg gctactttga tgcaggaaca aaaaaagtta
     9241 ctgtaagagc cagcgacggg tctctttccg ctgtaagctc tttcaatata aacgttaaaa
     9301 atgtaaacag agcaccgtca atggcagtac cggatgaaac tgttgatgaa gcccagaccc
     9361 ttaccttgga tctttcaaag ctttcaagcg atccggataa ggatggcctt agttattact
     9421 ctgtaaacaa tgtaggatca ataagcggtt ctatctttac atataccact acctatgacg
     9481 acgccggaga aaagcttgcc agagtaggtg taagcgatgg tgagctcagt tacgaaacgt
     9541 atttcaaaat aattgtcaaa aatgtgaata gggcaccaca gatcagcata cctgatcaaa
     9601 cagcctttga gaacaacaag cttactttgg atctttcgaa gtactcaagt gatcctgata
     9661 acgacaagct ttcgtactca gtactgaacc agacaggtaa aatagaagga aactctttca
     9721 tatatacgcc gtcatacttt gatgcaggag aaaaagttgt ccagatcgca gcttttgacg
     9781 gaaaacttta tggttattca acctttaaga taactgtgaa caacgtaaac cgtgcgccgt
     9841 caatctttat acctgaccag gagatagatg agggaaagac acttaacatt aatctttttg
     9901 aactttcttc ggatcttgac ggagatactt taaaatattt cataggaaac ggtataggaa
     9961 gcatcaacgg gaataactat acctactctc cagctttcag cgatgccgga ataaaaattg
    10021 ttgaggtaat agcttttgac ggtatcgata gaacagccgc caaatttaag ataaacgtca
    10081 aaaaaggaaa ccggacacct attatttcaa ttcctaacca gactgtgaat gaaggtcaga
    10141 ctttagctct tgatctttca aaactttcaa gcgatccgga cggagaaagg cttacttatt
    10201 ccttagaaaa tggcgtagga gagataaagg gaagaattta cacatactcc ccagcataca
    10261 atgaagccgg tgagaaaaca gtaaaaataa aagtcagcga tggaatttca ggtgttgaaa
    10321 ccactttcaa agtatatgta agcaacgtca acaagcctcc tgttttctca atacccgata
    10381 tattcataca ggaaggaaag actgtatcaa tagaactcaa agactacacg aaagatcctg
    10441 acggcgattc aattacattt gctttcgaaa acggagttgg taaagttgaa tctggaatat
    10501 ttatatatac tccttcttat gaagaggccg gtcagcagca ttgtcagatt ttaataagcg
    10561 acggagcggt gactgtcggc
//
LOCUS       DAOSHR010000062        10560 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_5302_length_10560_cov_6.320228, whole genome shotgun sequence.
ACCESSION   DAOSHR010000062 DAOSHR010000000
VERSION     DAOSHR010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 10560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10560
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5302_length_10560_cov_6.320228"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="PLU28_04790"
     CDS             <1..227
                     /locus_tag="PLU28_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34416.1"
                     /translation="DAININFTTNETIKYYRVDYTRSDGNPETITKENVSVNTFSVSN
                     ALTDASERTISVLVTDLAGNSKSYSYKVKK"
     gene            complement(282..1286)
                     /gene="corA"
                     /locus_tag="PLU28_04795"
     CDS             complement(282..1286)
                     /gene="corA"
                     /locus_tag="PLU28_04795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HOG34417.1"
                     /translation="MIDSNEKNINIELIEYSEQFYSSVHADSITQIQSSMHKQTIKWI
                     NVEEVTDNEAISAIGEMFSIHPLTLEDISNTDQRSKLEEYDSYIYMVLKMLSFDEKTS
                     APCIEQVSIILGKDFVITFQEGKRGDVFENIRQKISSGKSKARKLQTDYLAYLIIDAI
                     VDNYFNILEKIEDNIEFLEELLTDNATGETLKIIDRSRRDMLYLRKAVWPLREVMSLM
                     THADNDLIKKSTVLYFRDVYDHTVQVIDSIETYRDIFSGMIDVYLSNVSNRMNQIMKV
                     LTMISTIFMPLSFLTGFYGMNFKYLPGLDSRLSVVLITSIILLIGFGMFFYFKKKKWF
                     "
     gene            1403..2302
                     /locus_tag="PLU28_04800"
     CDS             1403..2302
                     /locus_tag="PLU28_04800"
                     /inference="COORDINATES: protein motif:HMM:NF021445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HOG34418.1"
                     /translation="MIYKDIELSDKPLFEGYCNIVPNELSDYVFTTLFMWKDTYNIKY
                     TIFKDWLVMRMALPMEKEIFLMPKGKGDIKEVMNFIEQQSKDMDIPFRIFAVSAAEKQ
                     AIEESMPQRYSITPCRDYFDYVYSVKDLISLSGKKYHGKKNHLNTFMANDGISYENIS
                     SENLDQVVQAEEEWCKKHDCGKGETDLKSEKIAIFRALENFDKLGLTGGILKISGKIV
                     GFTFAEPISGDTVLIHIEKADPDTKGAYTAINQVFLEKQWKHMSYVNREEDLGLEGLR
                     KAKESYHPVKMIEKYGCELNDKV"
     gene            2374..4191
                     /locus_tag="PLU28_04805"
     CDS             2374..4191
                     /locus_tag="PLU28_04805"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme NO-binding domain-containing protein"
                     /protein_id="HOG34419.1"
                     /translation="MKGMVVGSWIETWKKLYGEKAVDSAMQRVGIEKDRVFTPLEDVP
                     DADVYNMLEVFLKSSGKTRDEVLKETGKENIYQFYRYYPNFFKKTSLLSFMSAMNDVH
                     ASLTRRMKNARPPLLDFEIIGDNEATIKYSSFRDMRAYFLGLLEGSSKYFNDPIVYDI
                     IEQSSDSKGSFIKIKVKASKPYARIKKMTFFSILSLGIFKSMLSSSAFHITILTFILS
                     LVFNMLTDNTVISAALTALSAGLVLFMAGKYFEKGLLNIRQAMRSMREKDFEKPVLIK
                     GEKELKEQSDELNSLRKDMTKMFIDVMGDVEEVDIFSSKVAQKARDMQNLADTMGELV
                     SQVADSSVQITSDAESISNIVDSNVGSIRNIISQQGIMVKSLDEAVKKITGSSEKVEE
                     SSQDIFEMSKRFNELVRVGRVLEGDAQKIMGVVDTVTSIAEETNLLALNAAIEAARAG
                     EAGKGFAVVAASIRKLAEGSKGAAEQIAQILSKISSGINKLTENMYNEFENMGVHAEK
                     LKESSDNNQVASQNIRKISDDLERILKELDNEGEKLQGITSSVQSLLAISEEGSATAE
                     EISASVRDFLKNIKGILDDLDKTNGFIKSFKDNFEKIKF"
     gene            4273..4782
                     /locus_tag="PLU28_04810"
     CDS             4273..4782
                     /locus_tag="PLU28_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOG34420.1"
                     /translation="MVKRPMVRQFLEDAFCGESKAHMKYSIFAEKAEKEGLHNLAKMY
                     RAIAYAEFVHAKNHFIALGHLSDITENLKSSIEGETFEVDNMYPVYLNASEFEKEKEA
                     ERSNHFALEAEKGHIVMYKEALEKAEKNQDVKSDTFYVCPICGYTTDEPVQDKCPVCG
                     AKKEFFVKF"
     gene            4794..5153
                     /locus_tag="PLU28_04815"
     CDS             4794..5153
                     /locus_tag="PLU28_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II SORL domain-containing protein"
                     /protein_id="HOG34421.1"
                     /translation="MMKLGDVVRTADFKAEKHVPVIECPDKAAMNVPFNVTVSVGKEI
                     SHPNTTEHHIKWIDLYVHYENDPNTVHLGRYDFGPVVSDPVVTVKVKLQKKGTLIALS
                     YCNLHGLWESKKEISLE"
     gene            5168..5329
                     /locus_tag="PLU28_04820"
     CDS             5168..5329
                     /locus_tag="PLU28_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOG34422.1"
                     /translation="MKKYRCLICGYIYDPEQGDTDNGIKAGTAFEKLPSDWVCPLCGA
                     GKDDFEPID"
     gene            5394..6644
                     /locus_tag="PLU28_04825"
     CDS             5394..6644
                     /locus_tag="PLU28_04825"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HOG34423.1"
                     /translation="MSCERTIEITDGVYWTGTLNPLLRVFDIIMKTEYGTSYNSYIVK
                     GSSKIALIDGGHEKFTDLFLEKVSGIIEGDFTKIDYVVVNHTEPDHAGNLFKLMELNP
                     EIKIVCTVPAKKFLEQISNRSFNFILASDELKIDLGGKTLEFLLSPFWHWPDTMFTYL
                     KEDKILFSCDGFGAHFSDERMFVDLLDEDSYKKYLQAMQYYYVHIMGPFKEHTRNTLE
                     KLDRYDIKTIAPSHGPIHTGEFVKKHTDLYRQWAKKDVHKKNRILLLYVSAYGYTRKL
                     AMSIAKGIENAGGEVQLFDLVHDVTAENKLDILVSKQEWADGILYGSPTINGDALLNI
                     WEAIGLLALVDGKKEKSYSAFGSYGWSGEAVPFLFNRLSAMKLDVFSSENPLRIRFKP
                     TEKDLETAEQFGRSFLEHIRGMEK"
     gene            6649..7476
                     /locus_tag="PLU28_04830"
     CDS             6649..7476
                     /locus_tag="PLU28_04830"
                     /inference="COORDINATES: protein motif:HMM:NF014090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HOG34424.1"
                     /translation="MSIIEKLDKSRFKKARKAFEERDVQQHILSHSHENIAVEPWHKT
                     SQGRYIAPAVYGASDGIVTTFAVVAGAQGAGLSPLVVLVLGFANLFADGFSMAVGDYI
                     SDKSEREYIKTEKAREEWEVKVNPEGERDELREIYRRKGVKEEELESFLDTITSNHDL
                     WIDTMMHEELGLMEDNEGSPLKSAMVTFFSFVIAGFMPLMAFVFSSFISFFADNMFLS
                     ASIITALTLFAVGALRYFVTGKKWIISGLEMMVTGGLSAAVAYLVGFIFKAVFNVQV"
     gene            7490..10240
                     /locus_tag="PLU28_04835"
     CDS             7490..10240
                     /locus_tag="PLU28_04835"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOG34425.1"
                     /translation="MKSIILIFIIIILSVLGISAATLTKEQKIEDFEYLYNMVSQNYP
                     YLWVKERQFGYDWTSMKDEFMREVENSEDDQTFIKCISRIINLLQNDHTKVLTYEELS
                     KYSQKYDSGFYAAVFSSKTISSYQKYDILKSSAEKKLIPFFDALYIDGFYKVVSSSDE
                     KLIPTGAIITRIDGIDADSYVLGLNSVVKLKKDFEDKGKFFCYMLPLERKEKVSIEYL
                     FNGNHIQTDITPYTEDYKTVFGDYKTVTAESDENILYEISSDGRQAYLKINSFDYSNI
                     KADKDKLFSFYSGIKNCKTLVIDIRGNSGGTDSYWTDLIVSPLAKHPYAVNSYIFIRS
                     GTYAQQAAAERFSLAYNFFQTSRSEVLGMKNLPPELLSEEFGPPLKIQRLVLPGMDGF
                     DAKIVVVTDKYVYSSSETFAYFCKKTGFATVVGERTGGDGIGFDPLFFVLPNSRLVVR
                     MTYTYGINPDGTSNEEYRTAPDFFIKISNDESIFSQISNIPKMAVQNWATEAFTGYFP
                     NNPLSFRALSAGESKLTGPVIIDKVKIKGAYRTDIKLIEEKVGFKSGDLIDPTMLKEL
                     NNNLKLMNSFSEVYAFIKESDLGKYELIIYINESSGLFKSSYEMFSTLLSDILGKSLT
                     LKSYNLFGKMINLSARYSFNKYKSSYLTTTYPSIFFKEMKYDLSAGFQSVKLQPTLGE
                     FAASNADLNINFLSYQGSRYYCDNLFAFQIKLIDERVASQSNLSLKDSLYLENTLRTA
                     GYFSSQTYDFSYWLRAGSLTDISNISSFKDLGEIYKKTNYLTEGLIDMKLSQGLLFSH
                     LTMAGGYRTEGTPFEHLFRVDSESWFKGYKGYFMSAGYVAATLRTGVILFDVLGLYGL
                     IDGGKIFSGWDQACSLSGLMIDAGFSIILYNPFADLEFYLAKDILSDEPFKMAVSFST
                     EY"
     gene            complement(10266..>10560)
                     /locus_tag="PLU28_04840"
     CDS             complement(10266..>10560)
                     /locus_tag="PLU28_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34426.1"
                     /translation="GGVIVLYVSNVNRSPAMALSDITIKVGETLTLDLLNYSKDSDGD
                     MIVYTVENGVGVVNGSIYSFAATEKDIGERLVVIRANDGTDYVDASFRLTVEK"
BASE COUNT         3452 a         1744 c         2255 g         3109 t
ORIGIN      
        1 ctgatgctat aaatattaac tttactacaa atgaaaccat aaagtactac cgtgtggact
       61 atacaagatc agatggcaat cctgaaacta taaccaaaga aaatgtaagc gtaaatacct
      121 tctctgtcag caatgctctt acagatgctt ccgaaaggac tataagtgtt ttggtaacgg
      181 atcttgcggg aaattcaaaa agctattctt acaaagtaaa aaaataactt ttatccaaaa
      241 aatatgctcc ccttagaagc aaaacttctc aggggagctt tttaaaacca ttttttcttt
      301 ttgaaataaa aaaacattcc aaagcctatc aaaagtataa tcgaagtaat cagtacgaca
      361 gaaagtcttg aatctagtcc cggcagatat ttgaagttca ttccgtaaaa acccgttaaa
      421 aaagacaaag gcatgaatat agttgatatc atcgtaagaa ctttcattat ctgattcatc
      481 ctgttgctta cgttggaaag ataaacatca atcattccgg aaaaaatatc cctgtaagtt
      541 tctattgaat cgataacctg aacggtatgg tcatatacat ctctgaaata aagaacggtt
      601 gattttttta taagatcgtt gtcggcatga gtcatgagag acatgacttc ccgcagaggc
      661 catacggctt tacgcaggta cagcatatcc cgccttgatc tgtctataat ttttagtgtt
      721 tccccagtgg cgttgtctgt gagaagttct tccagaaatt cgatgttatc ctctattttt
      781 tccagtatat tgaagtagtt atctactatt gcatctataa taaggtatgc aagatagtca
      841 gtctgtagct ttcttgcttt agatttaccg gaagaaattt tttgtcttat attttcaaaa
      901 acatctcctc tttttccttc ctgaaaggtg atgacaaaat cctttcctaa aataatgctc
      961 acctgctcta tgcatggagc gctggttttt tcatcgaaac tgagcatttt cagaaccata
     1021 tatatatagc tgtcatactc ttcgagtttt gaacgctggt cagtatttga aatgtcctcc
     1081 aatgtgagag gatggattga aaacatttct cctatggctg atatagcctc attatcggtt
     1141 acttcttcaa catttatcca tttaatggtt tgcttgtgca tagagctttg gatttgagta
     1201 atgctgtctg catgaacgga gctgtagaac tgttcagagt attctattag ctcaatatta
     1261 atgttttttt cattgctatc aatcaagacg ggcacctccg gcattaagat attaatatat
     1321 ttatataaag ttaatcacga agaaaaatta attatgtcta attatatcgg attattttgt
     1381 tgatttttta aaaggaggaa gtatgatcta caaagatatt gagctatcag acaaaccact
     1441 ttttgaaggt tattgcaata ttgttccgaa cgagctttcc gactatgttt ttacaacttt
     1501 gtttatgtgg aaggatacct ataatataaa gtataccatt tttaaggatt ggctggttat
     1561 gagaatggcg cttcccatgg aaaaagagat ctttcttatg ccaaagggga aaggcgatat
     1621 taaagaagta atgaatttta tcgaacaaca gtctaaagat atggacatac cgttcagaat
     1681 ttttgcggtt tcggctgccg aaaaacaggc catagaagaa tctatgccgc aaagatacag
     1741 cataacacca tgcagggact actttgacta tgtctattcg gtaaaagatc ttatatctct
     1801 ttcgggaaag aagtatcatg gaaagaaaaa tcacctgaat acttttatgg ccaatgacgg
     1861 gatttcatat gaaaacatct cctccgaaaa tctggatcag gtagtccaag cagaagaaga
     1921 atggtgtaaa aaacatgatt gtgggaaagg ggaaacggat ctgaagagtg aaaagattgc
     1981 catattccgt gctctggaaa actttgacaa gcttggactt actggaggca tactcaaaat
     2041 ttcaggaaaa atagtaggat ttacttttgc cgaacctata tccggcgata cagttctaat
     2101 acatatcgaa aaggccgatc ccgatactaa aggagcttac acagcgataa atcaggtttt
     2161 tcttgaaaag caatggaagc atatgagtta tgttaaccgt gaagaagatc ttggattaga
     2221 aggactcaga aaggccaagg aatcatatca tcctgttaaa atgattgaaa aatacggctg
     2281 tgagcttaat gataaagtat agcggcatta agaaaaaata tcgtgatata atgatattat
     2341 tgatattttt aatgtaatgg gaggatgatt accatgaaag gaatggttgt cggttcttgg
     2401 atagaaactt ggaagaaact ttacggggaa aaagctgtag attcggccat gcagcgtgtc
     2461 gggatagaaa aagacagggt ctttacgcca ctggaagatg ttccggatgc tgatgtttat
     2521 aatatgcttg aagtttttct taagtccagc ggaaaaaccc gtgacgaagt acttaaagaa
     2581 accggaaagg aaaatattta tcaattttac agatactatc cgaatttttt taagaagacg
     2641 agtcttcttt cgttcatgtc ggcgatgaac gatgtgcatg cttcacttac acgcagaatg
     2701 aaaaatgcaa gacctccgtt gcttgacttt gaaataatcg gcgataatga agcaaccata
     2761 aagtacagtt ctttcagaga tatgcgtgcc tactttttgg gacttttgga gggctcgtcc
     2821 aagtatttca atgatcctat tgtctacgat ataattgaac agagttcaga ttcaaaaggt
     2881 tcatttataa aaataaaagt gaaagcatcc aaaccttatg caaggattaa aaagatgact
     2941 tttttcagca tactaagttt gggtatattt aaatctatgc tatcttcatc tgcttttcat
     3001 ataaccattc ttaccttcat tctttctttg gtatttaaca tgcttactga taatactgtt
     3061 atttctgcag cacttaccgc attaagtgcc ggtttggtcc tttttatggc tggaaagtac
     3121 tttgaaaaag gtcttttgaa cataaggcag gcaatgagaa gcatgcgtga aaaggatttc
     3181 gaaaagcctg tcttgataaa aggagaaaaa gagcttaaag aacagagtga cgagcttaac
     3241 agcttgagaa aagatatgac gaagatgttc atagacgtta tgggggatgt tgaagaggtt
     3301 gatattttca gctcaaaagt tgctcagaaa gcaagagata tgcagaatct cgccgatact
     3361 atgggagagc ttgtatctca ggtagctgac agttcggttc agataacaag cgatgctgag
     3421 tcgatatcca atatagtgga ttcaaatgta ggttcgataa gaaatataat atcccaacag
     3481 ggaataatgg ttaagtcgct cgatgaggcc gtaaagaaaa ttaccggctc ctccgaaaag
     3541 gtagaagagt catctcagga tatattcgag atgagcaaaa ggtttaatga gcttgtgaga
     3601 gtcggaagag tgcttgaagg agatgctcag aagataatgg gagtggttga tactgttacc
     3661 agcattgccg aagaaaccaa tcttttggct cttaatgctg caatagaagc tgccagagcg
     3721 ggagaagccg gaaaaggttt tgctgtggtt gccgcttcga taagaaagct tgctgaagga
     3781 agtaagggag cagcggaaca gatagctcag attctttcaa agatttcttc aggaataaat
     3841 aagcttacgg aaaacatgta caatgagttt gaaaatatgg gagtacatgc ggagaagctg
     3901 aaagaaagct ctgacaataa tcaggttgca tcgcagaata tacgtaagat ttcagatgat
     3961 ctggaaagaa tactcaaaga gcttgacaat gaaggagaga agcttcaggg aataacctca
     4021 agtgtgcaga gccttcttgc aatatccgaa gaaggatcgg caaccgcaga ggaaatatct
     4081 gcttctgtca gagatttcct gaaaaatata aaaggaatac tggacgatct ggataagacc
     4141 aacggattta taaaatcgtt taaggataac tttgaaaaga taaagttcta aatagttact
     4201 tataagaaat gttaatttaa aaaatgaatt ttataattta agtgctgatg gaaaaataaa
     4261 ggggtaagaa atatggttaa aagacctatg gtaagacagt ttcttgaaga tgctttctgc
     4321 ggagaatcaa aagctcacat gaagtacagc atatttgcag aaaaggctga aaaagaagga
     4381 ctccataatc ttgccaaaat gtacagagcc attgcatatg ctgagtttgt acatgcaaaa
     4441 aatcatttta tagctttggg acatctttcg gatattaccg aaaaccttaa atcaagtatt
     4501 gaaggagaaa cattcgaagt agacaacatg tatccggtat atcttaatgc atcggagttt
     4561 gaaaaggaaa aagaagcaga aagatctaat cattttgcct tagaagcaga aaaaggccat
     4621 atagtgatgt ataaggaagc tctggaaaag gcagaaaaaa atcaggatgt aaagtccgac
     4681 actttttatg tatgcccgat atgcggatat actaccgatg aaccggtaca ggataagtgt
     4741 cctgtatgcg gagcaaaaaa ggaatttttt gtaaaattct aaaagggggt attatgatga
     4801 agctaggaga tgttgtgaga acggcagatt tcaaagcgga aaaacacgtt ccggtcattg
     4861 aatgccctga caaggcagcg atgaatgttc ctttcaatgt aactgtcagt gtaggaaagg
     4921 agataagtca tcccaacact acagagcacc acattaagtg gattgatctt tatgttcatt
     4981 atgaaaatga tccgaatact gttcatcttg gtagatatga ctttgggcct gttgtttcgg
     5041 accctgtagt gacagtaaag gtaaaacttc agaaaaaggg aactcttata gccctttctt
     5101 actgtaacct tcacggacta tgggaaagca aaaaagaaat tagcctagaa taaaagggga
     5161 tgagactatg aaaaagtaca gatgcttaat atgtggatat atatatgatc cggaacaggg
     5221 agatactgac aacggaatta aggccggaac cgcttttgaa aagcttccca gtgattgggt
     5281 gtgtccgctg tgcggagcag gtaaagacga ctttgaaccc atagactaaa ataaaaaaag
     5341 ggggattcta tccctctttt ttatgtatta taaaataacc ggaggtgtat tttatgtctt
     5401 gtgaaagaac aatagaaata actgacggag tatattggac aggtactctt aatcctcttc
     5461 tgagagtttt tgacataata atgaaaaccg agtacggaac gagctataat tcatatatcg
     5521 ttaaagggag cagcaagatt gcattgattg acggtggcca tgagaagttc accgacctgt
     5581 tccttgaaaa agtatccgga ataattgaag gggatttcac aaaaatagat tacgttgtag
     5641 taaatcacac ggaaccggat catgctggga atctttttaa gctgatggaa ctgaatccgg
     5701 agataaaaat tgtatgtact gttccggcga agaaattttt ggaacagata tccaacagaa
     5761 gttttaactt tattctggca agcgatgagc ttaagataga tcttgggggc aagactctgg
     5821 aatttctgct tagtcctttc tggcattggc cggataccat gtttacgtat cttaaggaag
     5881 ataagattct cttttcatgt gatggattcg gagctcactt ttcagacgag agaatgtttg
     5941 tggatctcct ggatgaagac tcatataaaa agtatcttca ggcgatgcag tattactatg
     6001 tccatataat gggccctttt aaggagcaca caagaaatac tcttgaaaag cttgataggt
     6061 atgatataaa aaccattgca ccaagccacg gacctataca taccggcgag tttgtcaaaa
     6121 agcatactga cctttaccgc cagtgggcaa agaaagatgt tcacaagaaa aacaggattc
     6181 ttctgctcta cgtttcagca tacggctata caagaaaatt agcgatgtca attgcaaaag
     6241 gcatagaaaa tgcaggagga gaagttcagc tctttgacct tgttcatgac gttactgctg
     6301 aaaataagct tgatatactt gtaagcaaac aggagtgggc tgacggtata ctgtacggat
     6361 ctcctacgat aaacggtgat gcgcttttaa atatatggga agctatagga ttgcttgcgc
     6421 ttgttgacgg taagaaggaa aaatcttact ccgcatttgg ttcatacgga tggagcggag
     6481 aagctgttcc gtttttattt aacaggcttt ctgcaatgaa gcttgatgtt ttttcatctg
     6541 aaaatccttt aagaataaga ttcaagccta cggaaaaaga tcttgaaacg gcagaacagt
     6601 ttggccgcag cttccttgaa catatacgtg gcatggaaaa gtgataaaat gtcgataata
     6661 gaaaagcttg ataaaagcag atttaaaaaa gccagaaagg ctttcgagga aagagatgtt
     6721 caacagcaca ttctttctca cagtcatgaa aatattgcgg ttgaaccttg gcataaaacc
     6781 tctcagggaa ggtacattgc accggcggtt tacggggcaa gcgacggaat agtaactacc
     6841 tttgcagtag tggccggtgc acagggggcc gggctttcac cgctggtggt gctggttctt
     6901 ggatttgcaa acttatttgc agacggattt tcaatggctg taggagatta tatatcagat
     6961 aagtcagaaa gagagtacat aaaaacggaa aaggccagag aagagtggga agtaaaggtc
     7021 aatccggagg gtgagaggga tgaactaaga gaaatttaca gaagaaaggg agtaaaggaa
     7081 gaagaacttg aaagctttct tgatactata acctcaaatc atgatctttg gatagatacg
     7141 atgatgcatg aggaacttgg acttatggaa gataacgaag gatctcccct gaaaagtgcc
     7201 atggtaactt ttttctcatt cgtaatagct ggttttatgc ctcttatggc atttgtgttt
     7261 tcatcgttta taagcttctt tgctgataac atgtttctat cggcaagcat aatcactgcg
     7321 cttactctgt ttgcagtagg agccctgagg tactttgtca ccgggaagaa atggattata
     7381 agcggtcttg aaatgatggt gacaggcggc ctttctgcag ctgttgctta tctggtgggt
     7441 tttatattta aagcggtttt taacgtccag gtatagatga ggcttaagta tgaaaagcat
     7501 tattttaatt tttataataa ttatattgtc ggttttaggc atctcagctg caactcttac
     7561 caaagaacag aaaattgaag attttgagta tctttacaac atggtttcac agaactatcc
     7621 atacctttgg gttaaggaaa gacagttcgg atacgactgg acatccatga aagatgagtt
     7681 tatgcgggaa gtagagaatt cagaagatga ccagaccttt ataaagtgta tatcccgtat
     7741 tattaacctt cttcagaacg accacactaa ggttttaaca tatgaagagt taagtaaata
     7801 ctcacaaaaa tatgacagcg gtttttatgc cgctgtattt tcatctaaga ccatttcttc
     7861 atatcaaaag tacgatattt taaagtcatc agcagaaaaa aaactgattc cgttttttga
     7921 tgccctttat attgatggtt tctacaaggt tgtatcgtca tcagacgaaa aacttatacc
     7981 gacaggagcc attatcaccc gtatagacgg tattgatgca gacagttatg tccttggatt
     8041 gaactctgtt gtaaagctga aaaaggattt tgaagataaa ggtaagttct tttgctatat
     8101 gctgcctctt gaaaggaaag aaaaagtaag tatagagtat ttgtttaacg gcaaccatat
     8161 ccaaacagat ataactcctt acactgaaga ctacaaaaca gtctttggag actacaaaac
     8221 agttactgct gagagtgatg aaaatatttt atacgaaatt tcttcagatg gaaggcaggc
     8281 gtacttaaag atcaattcct tcgattacag caacataaaa gccgataagg ataagttatt
     8341 ttccttttat tccggcatta agaactgtaa aactctcgta attgacatac gtggaaacag
     8401 tggtggaaca gattcatact ggacagatct cattgtttcg cccttagcca agcatccgta
     8461 tgccgtcaac agctatatct ttatacggtc tggtacttat gcccagcagg ctgcggcgga
     8521 aagatttagc cttgcatata acttttttca gacaagcaga agtgaggtgc ttggaatgaa
     8581 aaatcttccg cctgaattgt taagtgagga gtttggtcct ccgttgaaga tacaacgttt
     8641 ggtacttcct gggatggatg gctttgatgc aaagatagta gttgttacag ataagtatgt
     8701 atactcttca tccgaaactt ttgcatattt ctgtaaaaaa accggatttg caacagttgt
     8761 aggcgaacgt acaggcgggg atggaattgg atttgaccct ctattttttg ttcttccaaa
     8821 tagcagactg gtagtcagaa tgacttatac ttatggtatc aatcctgatg gtacttcaaa
     8881 tgaggagtat agaacagctc cggacttttt tattaagatt tccaatgacg aatccatttt
     8941 tagccaaata agtaatattc ccaaaatggc cgtacaaaat tgggctacag aggcatttac
     9001 ggggtatttt cccaacaatc cattatcgtt cagagctctt tcggccgggg aaagtaagct
     9061 tacaggtcct gtaattattg ataaagtaaa aataaaagga gcttacagaa cggatataaa
     9121 gcttattgag gaaaaagtag gttttaaaag cggtgatctg atagatccga caatgctcaa
     9181 agagcttaat aacaatttaa agcttatgaa cagcttttca gaagtatatg cttttattaa
     9241 agaaagtgat cttggaaagt atgagctaat aatttatata aatgaaagct ccggactttt
     9301 taagagttcc tatgagatgt tttcaacgct tctttcagat atattaggaa aaagtctgac
     9361 gcttaaaagt tataaccttt ttggaaagat gataaatctt tcggcacggt attcgttcaa
     9421 caagtataaa agctcatatc ttacaacaac gtatcccagt atttttttca aagagatgaa
     9481 atacgatctc tctgcagggt ttcagagtgt aaaacttcaa cctacccttg gagagtttgc
     9541 agcaagcaat gccgatctga atataaactt tttatcttat caaggctcaa gatactattg
     9601 cgataacctt tttgctttcc agatcaagtt gatagatgaa agagtggcat ctcaaagtaa
     9661 tctttcttta aaagactcat tatatcttga gaatacatta agaacggcag gctatttttc
     9721 atctcagact tatgatttct cttattggtt aagagcaggc tctcttaccg atatatcaaa
     9781 tattagctct tttaaagatc tgggcgagat atacaaaaaa accaattatc tgacagaagg
     9841 gcttatagac atgaagttat ctcagggact tttattttcg catcttacta tggcaggtgg
     9901 atacagaaca gagggtacgc cttttgaaca tttgtttaga gttgatagcg agtcttggtt
     9961 caaaggatat aaaggttact ttatgtccgc cggatatgtg gcagcaacgt taagaacagg
    10021 agtaatactt tttgatgttt taggtcttta cggtctgatc gacggaggaa agatattcag
    10081 cggatgggat caggcgtgtt ctttaagcgg actaatgatt gatgcaggat tttcaattat
    10141 actgtacaat ccttttgctg atttggagtt ttatctagcc aaagatattt taagtgatga
    10201 gccgtttaaa atggctgtga gtttttctac ggaatactag taaaaacccg tccttaagga
    10261 cgggtttatt tttctacggt taaacggaag gaagcatcaa cataatcggt accatcattg
    10321 gctcttatta ctaccagtct ttcaccgata tctttttctg ttgctgcgaa agagtatatg
    10381 gagccgttca ctacaccaac tccgttttcg actgtataaa ctatcatgtc gccgtcagag
    10441 tctttagaat agttcagcag atcaagggta agtgtttctc ccactttaat ggttatatcg
    10501 ctaagggcca tagcaggaga tctgtttacg tttgaaacat ataatacaat tactccgccg
//
LOCUS       DAOSHR010000063        10513 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_5347_length_10513_cov_5.823484, whole genome shotgun sequence.
ACCESSION   DAOSHR010000063 DAOSHR010000000
VERSION     DAOSHR010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 10513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10513
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5347_length_10513_cov_5.823484"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(122..601)
                     /locus_tag="PLU28_04845"
     CDS             complement(122..601)
                     /locus_tag="PLU28_04845"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOG34427.1"
                     /translation="MGISFVPSIIDDFVLKDYKNEDIKSIDFRGKWIVLYFYPKDGTA
                     GCTKEANDFSSLIDDFLKEETVVIGISPDKTETHKKFILKNNLKIILLSDPEKVLIEK
                     LGVWQLKQMYGKQYYGVSRTTFILDSGMNVRYVWKKVRVNGHAQEVLNKLIELKANK"
     gene            complement(605..1228)
                     /locus_tag="PLU28_04850"
     CDS             complement(605..1228)
                     /locus_tag="PLU28_04850"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HOG34428.1"
                     /translation="MIDFEKSIEKKFPVFEMEIEFLQKYKEYSVTLPLINVSGRYHIL
                     FEIRSQSLYEQPGEVCFPGGRVEKDEYPIQAALRETYEELGIKPVDIKIIGRMKPWIP
                     QFMSVIHPFIAVLKKDDFHINPMEVEDIFCVPVEYFFNTVPEQGCTEIVVKPSENFPY
                     HLIQAGRDYKWRNVSMPVYFYKYKERIIWGLTAFITKVFSQMLKDEV"
     gene            complement(1221..2096)
                     /locus_tag="PLU28_04855"
     CDS             complement(1221..2096)
                     /locus_tag="PLU28_04855"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOG34429.1"
                     /translation="MVYIIGLICDSGCDLPQEYIKQNGIFSVKLKVILDDVMYKDLEE
                     VQEETVIDYMKTKFPKTSLPSHAEVKEGFTELIKSGCNEIIGITISGKLSGTNNMFNL
                     VAKELMQENKDLCIEIIDTLSISIGTGLYVYKTAELIKKGRSFSEIIKNVKESIPKKV
                     KVFFTIPTLKYLKAGGRIGKVMGTLGDIINIKPVISVNEEGIYYTVSKDRGMKASVES
                     MYKHFEKFVENQKFVVAVYKAGVQPITNQFVQNILDNVQKMKNMTSKFTGTISSALLV
                     HTGDGLVGIAALIDD"
     gene            complement(2102..2356)
                     /locus_tag="PLU28_04860"
     CDS             complement(2102..2356)
                     /locus_tag="PLU28_04860"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HOG34430.1"
                     /translation="MLLYGRVQGVGLRYFAAQLASKYSMCGYVKNNSDSSVEIMLQGS
                     DDNIRRFRNELFHGNGIIRIEDIKEERVDGESLKDFTIRY"
     gene            complement(2376..2732)
                     /locus_tag="PLU28_04865"
     CDS             complement(2376..2732)
                     /locus_tag="PLU28_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34431.1"
                     /translation="MDNLRKNFKDILEFKNIENAYKSYIDSNGKIPVEELYIDDNLKE
                     KLKKVIEDDKGFIIMNTDEKESSEALDVGDVYQLVEELNALGFATNFIFSYNLKDLII
                     KVRKVAPDNTDEGMKW"
     gene            complement(2829..4289)
                     /gene="guaB"
                     /locus_tag="PLU28_04870"
     CDS             complement(2829..4289)
                     /gene="guaB"
                     /locus_tag="PLU28_04870"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOG34432.1"
                     /translation="MFRETLTFDDVLLIPKYSEVIPSQVDTKSLFVKDIYLKVPFISA
                     AMDTVTESGMAKAMAREGGVGIIHKNMSIDEQVHEVVKVKRAENGVIYDPITIGPKMP
                     VFEAEKIMQEYRIGGLPVVDKEGKLLGILTNRDIRFERNSRKLVSELMTPYKDLIVAG
                     VKTGIDEAKEILHINKIEKLPIVDDNQKIAGLITIKDVMSVIEHPFASRDKKGRLLVG
                     AAIGVSDGVERAKKLIEASVDVLVLDSAHGHSMNIIQTLKKIKSLFPETPVIAGNVAT
                     KEGAADLINAGADAIKVGIGPGSICTTRIIAGIGVPQLTAISDVYEVAKTYKIPVIAD
                     GGIRYSGDAVKALAAGANTIMMGSIFAGTDEAPGETIIYQGRKFKTYRGMGSISAMKK
                     GSKDRYFQADVEDAEKLVPEGVEGMVDYKGGVKDVIYQLVGGVKSGMGYVGAENITQL
                     VEKAEFIKISSAGVKESHPHDITITKESPNYYYPGK"
     gene            complement(4329..5279)
                     /gene="trxB"
                     /locus_tag="PLU28_04875"
     CDS             complement(4329..5279)
                     /gene="trxB"
                     /locus_tag="PLU28_04875"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOG34433.1"
                     /translation="MFFDISGISKRSSQQYDIVIIGGGPAGISAAIYASQGGLKPLLI
                     EKTIEGGQMNLTQSIENYPGILSIDGTVLSKDMAAHARAFGTEFEFSQVKEILLEGDT
                     KTVILESAEKITAKVIIVASGANPRFLGAKNEQKYISKGVSYCASCDGHFFKNKKIAV
                     VGGGNTAVQESIFLSKIAQEVVLIHRRDKLRADKIYQDRIFTISNIRYLWDSQVVEIN
                     GDTNVESLMIKNVKDQSVISERFDGVFIFVGAAPSTSFIKEIVKLDQEGYIITDENME
                     SSVSGIYAVGDVRHKELRQIVTAVADGAIAAAHAVRKYFA"
     gene            complement(5289..5969)
                     /locus_tag="PLU28_04880"
     CDS             complement(5289..5969)
                     /locus_tag="PLU28_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOG34434.1"
                     /translation="MDRLLDKKTSEQVGELLNKMTGKVEIIVFKGNNEFSAVAEQLFT
                     EISQITDKVKVISYDITSPVSDEFEIDKLLTPASVMLDSQGNDRGVRFYGIPSGHEFS
                     TLLQDLLAMSKNGEVEFSQKNIDRIKTIDKKVRIRVFVTPTCPYCPKAVISAHQTAMA
                     NSEMVVAEMFEANEFGNESQKYNVSSVPHTIIEVKENDGWVKTGEFIGAYPEDNYISE
                     LMQAIDTK"
     gene            complement(5989..6504)
                     /locus_tag="PLU28_04885"
     CDS             complement(5989..6504)
                     /locus_tag="PLU28_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34435.1"
                     /translation="MSLLVSLIVNFLSAVLSYYLIQDLILKNIKIPDFLEFFNSYGLM
                     SVVLLLFYLKNRYQIFLFEGVILLVFLLYYLRSYNAARKKFHEKFKAMILSFGYTREG
                     YFDVFLSKKLLIRGVESFFFGLGVYYMFDSFFRQFLMQSNLHTIVFCSILFAAAAQVK
                     NNKGLKIYKNN"
     gene            complement(6514..7422)
                     /locus_tag="PLU28_04890"
     CDS             complement(6514..7422)
                     /locus_tag="PLU28_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOG34436.1"
                     /translation="MLIALAIVIVLIFILVANSLRIIRPYEKGLVERLGKFNREIGPG
                     LNVIVPFIERVLKLDLREMVIDVPPQEVITKDNVIVTVDAVIYYQVTEAFRVIYNVGN
                     FEIAAIKLAQTNLRNVIGELELDQTLTSRERINTKLREVLDEATDKWGVKVTRVEIKK
                     IDPPNDIMEAMSRQMKAERTKRASILEAEGYKQSEILKAEGDKTAAILKAEGEAESKK
                     RVAEAERFKLIAEAEGQAQAIISVFKSIHEGNPTKDIITIKYLEALKEMSQGSATKIF
                     MPYEVSGILSGVAAMLETNKTEPEKK"
     gene            complement(7438..7872)
                     /locus_tag="PLU28_04895"
     CDS             complement(7438..7872)
                     /locus_tag="PLU28_04895"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOG34437.1"
                     /translation="MADWTLWLLFSVVLAIVELITPTFFLLWFSIGALAACISSVFIV
                     HDYVNVIIFLTISILLWLSTRKIVKKLSKTESPKTLYQDDIQGKTAKIKSVSDDGSFI
                     VTVLGEEWKALAVNSSEKQEMACGSIVKVIKRESNILYIQRL"
     gene            complement(7885..8715)
                     /locus_tag="PLU28_04900"
     CDS             complement(7885..8715)
                     /locus_tag="PLU28_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOG34438.1"
                     /translation="MEFIIQSKALYSTWVYYKPNRILFDCGEGISEFLGNKIYGIDSI
                     YITHSHIDHISGLWGLVNIRNNGMGSREKPLQIYYPSGARQFEEYMTFIFKMNKRLRY
                     DIITTPLELSQTVSISPSRYIKPFRTKHTPGETSYGFQIIEIRNRLKEAYRSLSQEEI
                     KKNVELLGKAAITENYESKVLTLSGDSLAIPTECALGSEILIHECTFFNENDRKIRNH
                     TSLRELTELISKTHPKRVIIYHISSRYERILLKKEKELKELFPEIDIVMIKTQGVTEI
                     "
     gene            complement(8729..9178)
                     /gene="rplI"
                     /locus_tag="PLU28_04905"
     CDS             complement(8729..9178)
                     /gene="rplI"
                     /locus_tag="PLU28_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOG34439.1"
                     /translation="MKVLLIKDLPKVGKKGEVIEVSDGYGRNFLIGKGYATKADEGEV
                     RHFQNLKESEEKRKEQQKKKNEVMISELQKNCYEIKVKAGENGKLFGAVTANDVALCI
                     KNKCGIEFDKNDFDEKINIKEIGTYRIKIKFSQGVRGEIALKILPLS"
     gene            complement(9242..9472)
                     /gene="rpsR"
                     /locus_tag="PLU28_04910"
     CDS             complement(9242..9472)
                     /gene="rpsR"
                     /locus_tag="PLU28_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOG34440.1"
                     /translation="MTFIRKRKMPNKKCKLCAMKIQYIDYKDLNMLGDFVSEKGKIMP
                     KRINGNCAKHQRMVRNAVQRARHMCLVPYTKD"
     gene            complement(9469..10023)
                     /locus_tag="PLU28_04915"
     CDS             complement(9469..10023)
                     /locus_tag="PLU28_04915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOG34441.1"
                     /translation="MAISFNKVILVGRLTRDPEIRSTVSGTRVANFSLAVDRGYRKNE
                     NSDSVNTDFFRIVAFNTLADFASTYLKKGRLVLVEGSLRINKYKDKNDISRESVEVWA
                     SSVTFMESKKDNAYTDGYAEITNSDINVEQASHSSVSYGNSKKIESIDDDIISNDIPE
                     FDIFNDLKENIANDDKPDIGGGKR"
     gene            complement(10026..10418)
                     /gene="rpsF"
                     /locus_tag="PLU28_04920"
     CDS             complement(10026..10418)
                     /gene="rpsF"
                     /locus_tag="PLU28_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOG34442.1"
                     /translation="MAKARIYELMFIVRTDLAQEKRDELVEKVKGWVTERVGGTFDSF
                     TRWGIRKLAFRTQLNLTDGDYSWCIFRADPDKVNELSGLMNVTPEIFRYQFFRREDLE
                     KNEKKKTQNITVEEPVKVENSEQTPIGE"
BASE COUNT         3056 a         2255 c         1478 g         3724 t
ORIGIN      
        1 actcttgaaa tacttcaaaa acttgaaaaa attggtgatc atatgcaagg cctctttatg
       61 cttgactctg aattatcaaa aagagaaggt taaatcaaaa ccgcccttaa caagggcggt
      121 ttcacttatt agctttcaat tctatgagct tgttaaggac ttcctgcgca tgtccattta
      181 ctcttacctt tttccaaaca tacctgacat tcatcccgct atcaagaata aaagtggtcc
      241 tcgatactcc gtagtactgt tttccataca tctgtttgag ctgccatacc cctaattttt
      301 caattagtac tttttcagga tcactcaaaa gtattatttt aagattattt ttgaggataa
      361 attttttatg cgtctcagtc ttatcgggac ttatgccaat aacaactgtc tcttctttca
      421 aaaaatcatc tataagactc gaaaaatcat tggcctcctt ggtacagcct gctgtaccgt
      481 ctttaggata aaaatagagg actatccact tacctctaaa atcaattgat tttatatctt
      541 catttttata atctttaagt acaaaatcat caataatact tgggacaaaa gatattccca
      601 ctacttacac ctcatcttta agcatctgag aaaaaacttt tgttataaat gctgtcaatc
      661 cccatattat tctttctttg tatttataaa aatacactgg catagaaaca tttctccact
      721 tataatccct accagcttga ataagatgat acggaaaatt ctcagaaggc tttaccacta
      781 tttctgtaca tccctgttct ggaacagtat taaaaaaata ttcaactgga acacaaaata
      841 tatcttcaac ttccatagga tttatatgaa aatcatcttt ctttagaacg gcaataaaag
      901 gatgaataac tgacataaac tgaggaatcc aaggcttcat acgtccaata atttttatat
      961 ctacaggctt tataccaagt tcttcatatg tttcacgcaa agcagcctga attggatact
     1021 catctttttc aactctgcct ccaggaaagc atacttcgcc aggctgttca taaagacttt
     1081 gacttctgat ttcaaatagt atatgatatc tgccggatac attaattaat ggtaaagtaa
     1141 cagagtactc tttatatttc tgaagaaact ctatttccat ttcaaaaaca ggaaacttct
     1201 tttctatgct tttttcaaaa tcaatcatct attaatgccg ctattcctac tagtccgtcc
     1261 ccggtatgaa ccagaagtgc tgaagatata gtacctgtaa actttgaagt catatttttc
     1321 attttctgaa cattgtccag aatattttgc acaaactgat ttgtaatagg ctgaacccca
     1381 gctttatata ccgctactac aaatttctga ttttcaacaa acttttcaaa atgcttgtac
     1441 atggattcta ctgaagcttt cattcctcta tcttttgaaa cagtatagta tattccttct
     1501 tcattaacag agataacagg ttttatgtta ataatatcgc cgagcgttcc cattactttt
     1561 cctattcttc caccggcttt taagtactta agggtaggaa tagtgaaaaa aactttcact
     1621 ttctttggaa ttgattcctt aacatttttt attatttcag aaaaacttct accttttttt
     1681 ataagctcgg ctgttttata tacataaaga ccagttccta tagaaatact caaagtatct
     1741 attatttcaa tgcaaagatc tttgttttcc tgcatcagct ctttggctac cagattgaac
     1801 atattgtttg ttccgctaag cttacctgat atggtaatgc ctattatctc attacaaccg
     1861 ctctttataa gctctgtaaa gccttctttc acctcagcat gagaaggaag cgaagtcttt
     1921 ggaaactttg tcttcatata atctataaca gtttcctcct gaacttcttc aagatcttta
     1981 tacatgacat catctaaaat aactttaagc ttaactgaaa aaataccgtt ctgtttaatg
     2041 tattcctgag gaaggtcaca gccagagtca caaattaatc cgataatata taccactccc
     2101 tttaatatct tattgtaaaa tcttttaatg attctccgtc gactctttct tcttttatat
     2161 cttcaattct gatgattcca tttccgtgaa aaagttcatt tctgaatctt cttatattat
     2221 catcacttcc ttgtaacata atttccacac tactatcaga attatttttc acatatccac
     2281 acatagaata ttttgaagcc agttgagcgg caaagtatcg caagccaact ccctgaaccc
     2341 ttccgtaaag aagcatttta tagctctgca tagctctacc atttcatgcc ttcatcagta
     2401 ttgtcaggag caacctttcg aacttttatt ataagatcct tcagattata agaaaaaata
     2461 aaatttgttg caaaacccaa agcattaagc tcctcaacca actgataaac atctcctaca
     2521 tccagtgctt ccgaactttc cttttcatcg gtattcatta taataaagcc tttgtcatct
     2581 tcaatgacct ttttaagttt ctctttcaga ttatcatcta tgtaaagctc ctccacagga
     2641 atctttccat tactgtctat atatgattta tatgcgtttt caatattttt gaactcaaga
     2701 atatctttaa aattctttcg cagattgtcc atgtttgttc acctccctgt aaatattata
     2761 catttatttt aatgaaattg caactatttc atcagctaac gtaacatata ataacaggaa
     2821 ggcagaactt atttgccggg atagtagtaa ttcggcgact ctttggttat ggttatatcg
     2881 tgcgggtgac tttctttaac ccctgcagag ctaatcttta taaactcggc tttttcaaca
     2941 agctgagtaa tattttcagc acctacatac cccatccccg attttactcc acctacaagc
     3001 tgatatatta catcctttac tccaccttta taatcaacca ttccttctac accctcagga
     3061 acaagttttt ctgcatcctc aacatcagcc tggaagtatc tgtccttgct tcccttcttc
     3121 attgcactta ttgagcccat acctctgtaa gttttaaact ttctgccctg gtatattata
     3181 gtctctcctg gtgcttcatc tgtccctgca aatatagaac ccatcattat agtatttgct
     3241 ccggcagcaa gagctttaac ggcatcccca gagtatctga tcccaccgtc agctatcaca
     3301 ggtatcttat atgttttagc tacttcataa acatctgaaa tagcagtaag ctgaggaaca
     3361 cctataccag ctattattct tgttgtacat attgaaccag gacctatccc gacttttatt
     3421 gcgtcagcac cggcattaat aagatcagca gctccttctt ttgtggctac atttcctgcg
     3481 atcactggag tttcagggaa aagacttttt atttttttaa gtgtctgaat aatattcatc
     3541 gaatgtccgt gagccgaatc aagaaccaga acatcaacac ttgcttctat tagtttttta
     3601 gctctttcaa cgccatcact tactcctatg gctgcaccaa caagaagtct tcccttttta
     3661 tctcttgatg caaaaggatg ctcgataaca gacatcacat cttttatggt tattaatcct
     3721 gcaattttct gattatcatc aacaatagga agcttttcaa tcttattgat gtgaagaatt
     3781 tcttttgcct catcaatacc tgtttttaca cctgctacta taaggtcttt atatggagtc
     3841 ataagctcac ttacaagttt tctggaattt ctttcaaatc taatatcacg attggttagg
     3901 atacctaaca gtttgccttc tttatctact acaggcaaac cgcctattct atattcctgc
     3961 attatttttt cggcttcaaa aacgggcatt ttgggaccta tagttatcgg atcatagata
     4021 acaccgtttt ctgctctctt aactttcaca acctcatgaa cctgttcgtc aatgctcata
     4081 ttcttatgta tgattcctac gccaccttcc cttgccattg ccttagccat cccagactca
     4141 gtaacggtgt ccattgcagc cgaaataaac ggaaccttaa gatatatgtc ttttacaaat
     4201 aaacttttcg tatcaacctg agaaggtatt acttcactgt attttggaat cagcaataca
     4261 tcgtcaaaag tcagagtttc cctgaacaaa ttaaacaccc ctttttattt ttattctgga
     4321 aaattatttt atgcaaaata ctttctcact gcatgtgctg cggcaatagc tccgtcagca
     4381 actgcagtta ctatctgtct caattcttta tgccgcacat ctcctacagc atatataccg
     4441 cttacagaac tctccatatt ttcatccgtt atgatataac cttcctgatc aagcttaact
     4501 atttctttaa taaaagatgt tgaaggagca gcaccgacaa aaatgaaaac accgtcaaat
     4561 ctttcagata taacgctttg gtctttcaca ttttttatca taagcgattc tacatttgta
     4621 tcgccgttta tctcaactac ctgtgaatcc cataaatatc ttatgttact tatcgtgaat
     4681 attctgtctt gatatatctt atctgcacgt aatttatctc tgcggtgtat aagaacaact
     4741 tcctgagcaa tcttagaaag aaaaatgctc tcctgtacag cggtattccc tccacctact
     4801 acagcaattt ttttgttttt aaaaaagtgc ccatcacaac ttgcacaata agaaacccct
     4861 ttacttatat atttttgctc attttttgcc cccagaaatc gtgggtttgc tcctgaagca
     4921 acgataataa cttttgcagt aatcttttct gcagattcaa gaataactgt tttggtatca
     4981 ccttcaagca gtatttcttt cacctgcgaa aattcaaatt cagttccgaa agcacgcgcg
     5041 tgtgcagcca tgtcttttga aagaacagtc ccgtcaattg agagtatgcc aggataattc
     5101 tcaatacttt gtgtgaggtt catctggccc ccttcaatag ttttttctat taaaagtggt
     5161 tttagacctc cttgagaagc ataaatagca gcacttattc cggcaggccc tccacctata
     5221 attacgatat cgtattgctg actggaacgt ttacttattc ctgaaatatc aaaaaacatc
     5281 tattcacctc atttggtatc tattgcctgc ataagctcag aaatatagtt atcttctgga
     5341 taagcaccta taaactctcc tgttttcacc caaccatcgt tttcttttac ctcaattata
     5401 gtatgtggaa ccgaacttac attatatttc tgagattcgt ttccgaattc attagcttca
     5461 aacatttcag ccacaaccat ttctgagttt gccatggccg tctgatgggc tgatataaca
     5521 gccttagggc agtatggaca tgtaggagtt acaaaaactc ttattcttac tttcttatct
     5581 attgttttta ttctatcgat atttttttgg gaaaattcta cttcaccgtt tttagacatg
     5641 gcaagcaaat cctgaagaag agtggagaat tcatggcctg aaggaattcc gtaaaatctt
     5701 actcctctgt catttccttg agaatcaagc attacgcttg ctggagtaag taatttatct
     5761 atttcaaact catctgatac cggagaggtt atatcataag aaataacctt tactttatct
     5821 gttatttggg atatttctgt aaaaagctgt tcagcaaccg ctgaaaattc attgttgcct
     5881 ttgaaaacta ttatttcgac tttaccggtc atcttgttta aaagttcacc gacttgttca
     5941 cttgtttttt tatcgagcag cctatccatg aatttacctc ctgaaatttt aattattttt
     6001 atatattttt aatcccttgt tattttttac ctgtgcagct gcagcaaaaa gaatactgca
     6061 aaaaacaatt gtatgcagat tactctgcat tagaaactgt ctgaaaaagc tatcaaacat
     6121 atagtaaact cccaaaccga agaaaaatga ttcaactcct cttatcagaa gtttttttga
     6181 aaggaaaaca tcaaagtatc cctcacgagt atatccaaaa gaaagtatca tagccttaaa
     6241 cttttcatga aatttttttc tagccgcatt ataagatctc agataataaa gaagaaaaac
     6301 cagtaagata actccttcaa aaagaaaaat ttgataccta ttctttaaat aaaacaataa
     6361 caaaactacg gacataagtc cgtagctgtt aaaaaattct agaaaatctg gtatcttgat
     6421 atttttaagt ataagatcct gtataagata ataagatagt actgcagaga gaaaatttac
     6481 tattaaagat actaaaagac tcatttttgt tttttatttc ttttcaggtt ctgttttgtt
     6541 ggtttccagc atggccgcaa caccggaaag tataccagat acttcataag gcataaatat
     6601 ttttgttgca ctgccctgag acatctcttt aagtgcttca agatacttta tggtaataat
     6661 atctttggtt ggattacctt catgaataga tttaaaaaca gaaataatag cctgtgcctg
     6721 gccttctgct tcggcaataa gcttgaatct ttcagcttcg gccactcttt ttttggactc
     6781 ggcttctcct tctgctttaa gtatagctgc ggttttatcg ccttcagctt taagtatttc
     6841 tgactgttta tatccttcag cctcaagtat ggacgctctc ttggttcttt cggctttcat
     6901 ctgtctgctc atggcttcca tgatgtcgtt aggaggatct atctttttga tttcaactct
     6961 tgtaaccttt acaccccatt tatcggttgc ttcatcaaga acttctctca actttgtgtt
     7021 tattctttct ctggatgtaa gtgtctggtc aagttccagc tcaccaataa cgtttctcaa
     7081 attggtttga gcaagtttaa ttgctgctat ttcgaaattg ccaacattgt aaataactct
     7141 gaaagcttca gtaacctgat aatatattac tgcatcgaca gtaactataa cattatcttt
     7201 ggttataact tcctggggag gaacatctat aaccatctca cgaaggtcaa gcttaagaac
     7261 tctttctatg aatggaacaa taacgttaag accaggtcct atctccctgt taaactttcc
     7321 aagcctttca acaagacctt tctcataagg tcttataatc ctcagactgt ttgcaacaag
     7381 gataaaaatc aggacaataa ctatcgccaa ggctatcagc atactctttt acccccttta
     7441 aagtctctgt atataaagaa tgttactttc tctttttata acttttacta ttgagccaca
     7501 tgccatttcc tgtttttccg aagagttaac agccaatgcc ttccactctt cacccaatac
     7561 agttactatg aaacttccgt catcacttac agattttatc tttgcagttt ttccctgaat
     7621 atcatcctga tataaagtct taggagattc agttttagag agcttcttaa caattttgcg
     7681 tgttgaaagc cataacaata tggagattgt cagaaaaatt atcacgttca cataatcatg
     7741 aactatgaaa actgaactta tacatgctgc caaagcacct attgagaacc ataaaagaaa
     7801 aaatgtaggt gtaataagct ctactattgc aaggactact gaaaacaaca accacaaggt
     7861 ccagtcagcc atacttatgc ccccttatat ttcagttact ccctgagttt ttatcatcac
     7921 tatatcgatc tcaggaaaaa gttctttgag ttctttttct tttttcaaaa gaatcctttc
     7981 atatcggctg gatatatgat atattataac tcttttaggg tgtgttttgg atattagttc
     8041 tgtcaactca cgcaaagaag tatgattcct tattttcctg tcattttcat tgaaaaaagt
     8101 gcattcgtga ataaggattt cgctgcccag agcacattcg gtcggaattg ccagactatc
     8161 gccactcaat gtaagtactt tggattcgta attttcagta attgctgctt ttccaagaag
     8221 ctcaacattc ttttttattt cttcctggct gagactccta tatgcttctt taagtctgtt
     8281 tctgatttca attatctgaa aaccgtaaga agtctcgcca ggcgtatgtt ttgtacgaaa
     8341 aggttttata tatctgcttg ggcttataga aacagtttga gaaagctcaa gcggcgtagt
     8401 tattatatca taccgcagtc tcttgttcat tttaaatata aatgtcatat actcttcaaa
     8461 ctgacgtgca ccgctaggat agtatatttg taagggtttt tcccggctac ccattccatt
     8521 gttccttata ttaaccagtc cccataaccc tgatatatgg tcaatatgag aatgagtaat
     8581 atatatactg tcaattccat atatcttatt tcctaaaaac tcgcttatcc cttctccgca
     8641 atcaaaaagt atcctattcg gtttatagta tacccatgtt gaatacagag ctttagattg
     8701 aataataaat tccaaaatat acctccattc atgaaagcgg aagtattttc agagcaattt
     8761 ctcctcgtac tccctgagaa aattttattt ttattctgta tgttccgatc tcttttatat
     8821 ttattttttc atcaaaatca tttttatcaa actctatacc acatttattc tttatgcaaa
     8881 gagccacatc gttggctgta acagcaccaa aaagtttacc gttttctccg gcttttactt
     8941 ttatttcata acagttcttt tgaagctcgg aaatcattac ttcattcttc tttttctgtt
     9001 gttctttgcg cttttcctct gattctttaa gattttggaa atgcctgact tcaccctcat
     9061 cagcctttgt tgcgtatcct ttgccaataa gaaagtttct cccgtatcca tctgatactt
     9121 ctatgacctc tcctttttta ccgactttag gtaaatcctt tataagcaaa actttcattg
     9181 taatccacct ccattaatat tttaccataa aataaatggg aggcaaaaag ccccccaaca
     9241 attaatcttt ggtataagga accagacaca tgtgccttgc cctctgaaca gcatttctta
     9301 ccattctctg atgtttggca cagtttccgt ttatcctctt gggcattatc ttgccctttt
     9361 cacttacaaa atctcctagc atgttgaggt ctttgtaatc tatatactga atcttcattg
     9421 cacacaattt acatttttta ttcggcatct tcctttttct tataaaagtc atcgttttcc
     9481 acctccaata tctggtttat catcgttcgc aatattttcc ttaagatcat taaatatatc
     9541 aaattcaggt atatcgttcg aaattatatc gtcatctata ctttcaatct tctttgaatt
     9601 tccatacgaa acagaagaat ggcttgcctg ttcaacattt atgtcactgt tggttatttc
     9661 cgcataacca tcggtataag cattatcttt cttgctttcc ataaaagtaa ccgaacttgc
     9721 ccagacttct actgattcac gtgatatatc gttcttgtct ttatacttgt ttattcttaa
     9781 acttccttca acaagaacaa gcctaccttt tttaaggtat gtagaggcaa aatccgctaa
     9841 agtattaaaa gccacaattc taaaaaaatc cgtgtttaca gaatcgctgt tttcgttttt
     9901 acggtatcct ctatctacgg ccaggctaaa attggcaacc cttgtcccac taacagtaga
     9961 tcttatttcg ggatctcttg taagtctacc aaccaatatc actttattaa atgaaatagc
    10021 catacttatt ctccgatcgg agtctgttca ctgttctcaa cttttaccgg ttcttcaact
    10081 gttatattct gagttttctt tttttcattc ttttcaagat cttctcttct gaagaactga
    10141 tatctgaata tttcaggtgt aacgttcata agtcctgaaa gctcattaac cttatcaggg
    10201 tcagctctga agatacacca gctgtagtca ccatctgtaa gattgagctg tgttctgaac
    10261 gcaagttttc ttatacccca ccttgtaaat gaatcaaatg tacctccgac tctttctgtt
    10321 acccaacctt ttactttttc aaccaactca tctctttttt cctgagctaa atctgttctt
    10381 actatgaaca tgagctcata aatcctagct tttgccatgc ttacctcctc ccacggacta
    10441 taggctccct ttccgggagc gggttttatg ttacagtaac tattatatca gcatttttct
    10501 gttccagaac ata
//
LOCUS       DAOSHR010000064        10379 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_5450_length_10379_cov_6.528380, whole genome shotgun sequence.
ACCESSION   DAOSHR010000064 DAOSHR010000000
VERSION     DAOSHR010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 10379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10379
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5450_length_10379_cov_6.528380"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            63..422
                     /locus_tag="PLU28_04925"
     CDS             63..422
                     /locus_tag="PLU28_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG34443.1"
                     /translation="MKKILVVDDSEVLRNILTFNLKHAGYDVQEAINGKDAIDKIKKV
                     RPDTICLDIMMPIMDGFTVLKILKDEGMDIPVIVLTAKGGEDDEQNALSLGARKVLTK
                     PFSPKQLIDTVKSLVGE"
     gene            426..1817
                     /locus_tag="PLU28_04930"
     CDS             426..1817
                     /locus_tag="PLU28_04930"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOG34444.1"
                     /translation="MSDYKRIYNKLNSILNAFSDDTDDTAELQERVDKLIDIYINELN
                     NYKVKIEENTLILQAQFEEISRTYEELTTVLDISKMVFSVKDPRLAIENVVTRLKDVI
                     NFRNIVVGEFILNDDYHDFNLLYSEFLDADLNILPLLNIMNRTKPIKTILKERDEIND
                     YPDSMLLVPIKSQIKIWGFLLLYGKKDRSIFLASDKKILESVAEQLAFGFDTMNYLKE
                     RIKQQKMDEQLKLAKEIQESLLPKTLPTFEKIQLSTFYRSAFDVGGDYYDALKLSETK
                     LFAILADVSGKGVPAALIMSSLKGVVRSKVENAADLGEVVNYINNFLSRSLPSDRFVT
                     AIFVLVDSENKTLQVINAGHNESPFFIDGKFYSSKAGGLPLGILEDNAYSPEKFEYTE
                     DLVFFTYTDGLTEARNPKKDEYGWERLQSLMQLIKRDNTDVIVNSIIDSVDTFVENAE
                     QHDDTTLLAIKSF"
     gene            1830..2585
                     /locus_tag="PLU28_04935"
     CDS             1830..2585
                     /locus_tag="PLU28_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOG34445.1"
                     /translation="MSGHNKWANIKHRKGAQDAKRSQMFTKIIRELTVAAREGGAVPD
                     SNPRLRTAIEKAREANMPKDKIESAINKGIGAEGADALVEIMYEGYGPAGVALIISVV
                     TDNKNRTAQEIRHLLSRHGGALAENGAVSWNFERKAILEVPKNEITDSDAFLMAAIDA
                     GAEDVDDSDDPVEVVGPVESLGNMRNSIQNSGFTVKAQLSYLPKTTVKMTGSDAEKLL
                     KLLDSLDDNDDVQEVFGNYEIDEAEMEKLAQNM"
     gene            2634..3374
                     /locus_tag="PLU28_04940"
     CDS             2634..3374
                     /locus_tag="PLU28_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34446.1"
                     /translation="MKKNVFLICILLCSLSFFGRESSIIADYKNSLQINFNFNFDLPD
                     VYSGGLFYFIEFQNEKIKVSDIDQVINYIDFSQPGFEITYRYPKEIRSPTFIKTNYEF
                     FSVNADNFLNLSLKELFFGINFSDKLLKSSYWIKFTDIQTSEFNIGIDNNSLGLYLNI
                     GQNGVRPFFNLGKNIFFSVHDFFLDDLYIMTEEVIFRYSKSSGKLLINSLYFNYSNNI
                     FSYRIPINDKLMFVISDLGFGISFILKV"
     gene            3390..4202
                     /locus_tag="PLU28_04945"
     CDS             3390..4202
                     /locus_tag="PLU28_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HOG34447.1"
                     /translation="MEEGIVYESQYDDKDYERLLQDLIKYFQLDVTGYKQHRVRRRID
                     ILMKKYSYKTYSDYMKDLRNDPKKWDEFLDKLTINVTEFFRNPEKWEYLQKNIIPMLM
                     KENSSAFKAWSAGCSSGEEPYTLSIVFEEMRARNASILAADLDKFVLAKAQEGIYEER
                     STINMTTELKDRYFTKLADGKLQVKQLLKSRIKFKQLNLLTDPFEKNFDLIICRNVVI
                     YFDNDAKEGLYKKFAEALRPNGVLFVGSTERIFNYREIGLKTIAPFFYQKEK"
     gene            4202..4660
                     /gene="lspA"
                     /locus_tag="PLU28_04950"
     CDS             4202..4660
                     /gene="lspA"
                     /locus_tag="PLU28_04950"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF013421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HOG34448.1"
                     /translation="MSWIVPVIIFADQLIKVAAKNYLFYNPIKIWFFELTYTENTGIA
                     FGAFKGASLFFGVVSVVVAVIMLIARDRFYRQGKLSPLGDLAVNMIIGGAIGNMVDRV
                     RLGYVVDMFYIPFFSVFNVADSFVTIGAVLLAIYFLRTEKIGAKNTSNQQ"
     gene            4629..5558
                     /locus_tag="PLU28_04955"
     CDS             4629..5558
                     /locus_tag="PLU28_04955"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOG34449.1"
                     /translation="MEQKIPVTSNDVNKRLDLFICEKVPEWISRTFIQKSIKDGQILV
                     NSEPKKPGYRLRQDDVIVLELPEKPAPHEVTPQDIPLDIVYEDHDIIVINKEAGMVVH
                     PSGNCLKDTMVNALLFHCPEIQRDKNDIRPGIVHRLDKDTSGLIVVAKNEASFAGLSD
                     QFRDRTIRKSYLALVKGQLIENEGSIDAPIGRHPQIRIKMAVVVDGKPSLTEFKVLRR
                     FGSAATLVSVVLKTGRTHQIRVHFSYLGFPLMGDEVYGRANEDRKYDIFRQMLHAYRL
                     ELIHPATGRKMIFTAPLADDFCIGIKNVYRMVE"
     gene            5559..6002
                     /locus_tag="PLU28_04960"
     CDS             5559..6002
                     /locus_tag="PLU28_04960"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOG34450.1"
                     /translation="MSVLVVTFSSDNTVYFDKAIEIRNLVFENEQIYPPQFKKDSLED
                     LSDHFLLFANGIAVGTCRTRIIEENCMRIEKFAILKDYRSKGYGIFMISEIEKFSKSK
                     GCSRLVFFSPKALENYFIRLGFTPEEASAGSQSSYECKKMMKPVR"
     gene            6149..6934
                     /locus_tag="PLU28_04965"
     CDS             6149..6934
                     /locus_tag="PLU28_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOG34451.1"
                     /translation="MFSEKLGINYNISGSGKPIVLLNGIMMSIPSWTEHVKYLEKDFQ
                     VITYDMKDQGKSRYVDYQYDISMHVQDLKDLLTEIGIKKANLVGLSYGGQVAQLFALK
                     YPEMVDKLILSNTISHVDNFLKSIGQAWKTSAATYDGEFFFDVSLPFIYSRYFYQSNY
                     EWLMNRRKLFKATLTKQWFDGFIRLASSNETFDIREEIKNITAPTLLIGADEDIATPY
                     LEMLKMNEKIPQSQIICIPKTAHAMFLEKLPLFCDLVKGFLKE"
     gene            complement(6991..8124)
                     /locus_tag="PLU28_04970"
     CDS             complement(6991..8124)
                     /locus_tag="PLU28_04970"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOG34452.1"
                     /translation="MEDTRMKTKDRLIESALESFSERWYETVSVAEICRNANLSNGVF
                     YRYFKDKEEIFKEILDIFLKDLSENLEHIKGESVTQRLDYLFNAIVNSGVKFKRMVSV
                     YREGQYRFQEYEKKLADIYKKGLSEVFKKDISEAEYLFGTCGIRFLSIRSLYKHIVLD
                     NKILLQGEKMVLNGIFSNQVNNYEKIFDFDAKQLEAVQETTSREKLISAGMELFGEYG
                     YYNVNVYDIAKKAGFAVGTFYIYFDSKEEFMSELIDIINSKTRHFISQNINHSLNRIE
                     QELQGMYLFLKFFEDKLIYYEIVREAEFVVNGKVKDYYDNFEKGYLKNLKDVKDYDYS
                     AVANFLVGISHYLGIQTLFNRSAEDKKSIIKELGTLLYSGMEK"
     gene            complement(8168..9883)
                     /locus_tag="PLU28_04975"
     CDS             complement(8168..9883)
                     /locus_tag="PLU28_04975"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOG34453.1"
                     /translation="MNMKKALIVLIIGVLLGVLFINKSFLLMIFSLVLIYAIAATGLN
                     IVSGFTGQVSIGHGAFMAVGAYTTAAMAMDFGTPFYINILAAIVLAAFLGFLIGLPAL
                     RLKGFYLAIATMAFGVAVEQLFAAIEHFGGHIGMRNIPRFFESDFYIYLLNLVFFVVL
                     TYVASLILHSPVGEKYKMVRDSEVAARAYGIKPSRIKLNAFIVSAIYGGIAGALYSHT
                     VGYISPPDFGMNTSLNLLSMIVIGGMASLEGGMIGSIIITGLPFLFSRGNIPMTLIFG
                     ALLIVFVLFFPQGIAYGLKLLSMKYLGRPWALLVRKSWSRRAEDGKYVTVNGKKIFYR
                     QQGSGPAIVMIHGNFGSHRWYEKVSSIEGYTTYIPDLVNFGKSDRIDNVSIDTYADFV
                     KGFMDQLKIEKAVVVGHSLGGAVSMSLAFRYPQLVSKLVLVDSAPVNGLVTPEENFPV
                     LEMMKGNVSLLRNSLKGIMPACEDQKILSEVVKDALLMKDECFSENARALSRYDYTEL
                     AERYKNPVLFILGKKDMLITHDMAENTLKNLNGRLEVLDHVGHSVILEDSQLFKKLLI
                     NFVNL"
     gene            complement(9886..>10379)
                     /locus_tag="PLU28_04980"
     CDS             complement(9886..>10379)
                     /locus_tag="PLU28_04980"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOG34454.1"
                     /translation="LGEDGLLILPLNDLLITVISLGITILLAVFLKVTKVGIAIRARS
                     QDEVGSKVVGINTNMIDGLVWGIGISLSALVGVLIAPKTTVTPTMMINMQLYGFTAGV
                     LGGFTSLYGSILGGIILGILEKIVGIYISPDYQLTIILLIIILMLAFKPSGLFAKKFE
                     GRV"
BASE COUNT         3337 a         1952 c         1984 g         3106 t
ORIGIN      
        1 atttgaaccg gaaaaggatt tttttattcc aatatgcgat gaggccgtat ggagggctgg
       61 atatgaaaaa aatactggtt gttgatgatt ctgaagtatt gcgcaacata cttactttca
      121 atctcaagca cgccggatat gatgtacagg aagccattaa cggaaaagat gccatagata
      181 agattaaaaa agtacgtcct gataccatat gcctcgatat tatgatgcct ataatggacg
      241 gttttaccgt tctgaaaata ctcaaggacg aaggtatgga tattcctgtt attgtactca
      301 cagcaaaagg cggagaagat gatgagcaga acgctttgtc tttaggcgca agaaaagtgc
      361 ttaccaagcc tttcagtcct aaacagctta tcgataccgt caaatctctt gtcggagagt
      421 agattatgag tgattataaa aggatttaca ataaacttaa ctctattttg aacgcttttt
      481 ccgatgacac cgatgacact gcggagcttc aggaaagagt ggataagctc attgacatat
      541 acataaacga gcttaataat tataaagtga aaattgaaga aaacaccctc attcttcagg
      601 ctcagtttga agaaatatct agaacatatg aagagcttac cactgttctg gatataagta
      661 agatggtttt ctcagtcaaa gatcccagac tcgccataga aaatgtagtc acacggttaa
      721 aagatgtaat aaactttaga aatatagtag tgggagagtt tatccttaac gatgattatc
      781 acgactttaa tcttttgtat tcagagtttc ttgatgctga tctgaatata cttccgcttc
      841 ttaacataat gaacaggact aaacctatta aaactatttt aaaagagcgt gatgagataa
      901 acgattatcc ggactccatg cttttggtgc ctataaagtc tcagataaag atttggggat
      961 ttcttctgct ttacgggaaa aaagaccgtt caatatttct ggcctcagat aaaaaaattt
     1021 tagagtcagt tgccgaacag ctcgcattcg gatttgatac tatgaactat cttaaagagc
     1081 gtataaaaca gcagaaaatg gacgaacagc tgaaactcgc aaaagaaata caggagtctc
     1141 ttttacctaa gactttgccc acatttgaaa agattcagct ttcaaccttt taccgatctg
     1201 ctttcgatgt cggcggtgat tactacgatg ctttaaaact tagcgaaaca aagctttttg
     1261 ctattctcgc cgatgtttcc ggcaagggtg ttcctgctgc acttataatg agctctctta
     1321 aaggtgtggt gaggagcaag gtggaaaatg ccgccgatct cggtgaagtt gttaactata
     1381 taaacaactt tcttagcaga agtctgcctt cagatagatt tgtaactgcc atatttgtac
     1441 tcgttgattc agaaaacaaa acccttcagg taataaatgc cggacataac gaatcgcctt
     1501 tttttataga cggaaagttt tattccagca aagccggagg attaccgctc ggaatacttg
     1561 aggacaacgc ttactctcca gaaaaattcg agtatacgga agatcttgtc ttttttacat
     1621 ataccgacgg actgactgag gctcgcaacc ctaaaaaaga tgagtacggc tgggaaagat
     1681 tacaaagctt aatgcaactg ataaaaagag ataacacgga tgtaatagtt aatagtataa
     1741 tagattcggt agatactttt gtagaaaatg ccgagcagca tgatgataca actttacttg
     1801 caattaaaag tttttaggag ggatataaga tgtcaggcca caataaatgg gcgaatatta
     1861 agcacagaaa aggtgcacag gatgcgaaaa gatcgcagat gttcacaaaa attatcagag
     1921 aattgacggt tgcagccaga gaaggcggag ctgtacctga ttcaaacccg agattgagaa
     1981 ccgctataga aaaagcaaga gaagcaaaca tgcccaaaga caaaatcgaa tcggccataa
     2041 ataaaggtat tggtgctgaa ggagcagatg cgctcgttga aataatgtac gaaggttatg
     2101 gtcccgcagg tgtggccctt ataatttcag tagttacaga taataaaaat agaactgctc
     2161 aggaaataag gcacctgtta tccagacatg gcggagcatt ggcagaaaat ggagccgttt
     2221 cctggaattt tgagagaaag gctattctgg aagttccgaa gaatgaaata accgattccg
     2281 atgctttcct tatggcagcc attgatgcag gagcagagga tgtagatgat tcagacgatc
     2341 cggtagaagt tgtaggtccg gtagagtctc tgggaaatat gagaaactcc atacagaact
     2401 ccggttttac agtaaaagcc cagttgagtt atctgccaaa aactacagta aaaatgactg
     2461 gatcagatgc ggaaaaatta ttgaagcttc tggattccct ggatgacaac gatgatgttc
     2521 aggaagtatt tggaaattac gaaatagacg aagctgaaat ggaaaaactg gctcagaata
     2581 tgtaaataaa atggggacat cgtccccgtt ttgttttctt tggagtgtat attttgaaaa
     2641 aaaatgtttt tttaatatgc atcctgttat gttctttgtc tttctttgga agagaaagtt
     2701 cgattattgc cgattacaaa aattcccttc aaataaattt taacttcaat tttgatctgc
     2761 ctgatgtata cagcggcgga ttgttttatt ttatagaatt tcagaatgaa aaaattaagg
     2821 taagcgatat agaccaggtg attaactata tcgacttcag ccagccagga tttgagatca
     2881 cctaccgtta cccaaaagag atccgcagtc ccacttttat aaagaccaac tatgagttct
     2941 tttcggttaa tgctgataat tttttgaatc tgtctcttaa ggaacttttt ttcggtataa
     3001 atttttctga caaactgctg aaaagctctt actggataaa atttactgat atacagacct
     3061 cagagttcaa cattggaatt gataacaact cgcttggtct ttatcttaac ataggacaga
     3121 atggtgttag accttttttt aatctgggaa aaaacatatt cttttcagta catgattttt
     3181 ttcttgatga tctatacata atgaccgaag aagtcatttt cagatacagc aaatcatccg
     3241 gcaaactgct cattaactct ttatatttca attactcaaa caatattttt tcttacagga
     3301 tacctataaa cgacaaactc atgttcgtaa tttctgatct gggctttggc atatctttca
     3361 tactaaaagt ataaaaacgg aggtgtttta tggaagaagg aatagtatac gagtcccagt
     3421 atgacgacaa agattatgag agacttcttc aggatttaat aaagtatttc cagcttgatg
     3481 tgacaggtta taaacagcac agggtaagaa ggcgaataga tattttaatg aagaagtact
     3541 cgtataaaac ctactccgat tacatgaagg atctaagaaa cgatccaaaa aaatgggatg
     3601 agtttcttga taagctgacg ataaacgtta cagaattctt tagaaatcca gagaaatggg
     3661 agtatttaca aaagaatata attcctatgc ttatgaaaga aaactcttct gcattcaaag
     3721 cttggagtgc aggttgctct tcaggtgaag aaccttacac tctttcaatc gtctttgaag
     3781 agatgagagc aagaaatgcc tctattcttg ccgccgatct tgataagttt gttcttgcaa
     3841 aagctcagga aggaatatac gaagaaagaa gtaccataaa tatgactacc gagcttaaag
     3901 acagatactt tacaaaactt gccgatggaa agcttcaggt aaagcagttg ttgaaaagca
     3961 ggatcaagtt caaacagctt aaccttctga cagatccttt tgaaaaaaat tttgatctta
     4021 ttatatgcag aaacgttgtg atatactttg ataatgatgc aaaggaaggt ctctataaaa
     4081 aattcgccga agctctcaga cctaacggtg ttcttttcgt aggatccacc gaaaggattt
     4141 tcaactatcg tgaaattggt cttaaaacga tagctccgtt tttttatcaa aaggagaagt
     4201 aatgagttgg atagtaccgg taataatatt tgccgatcaa ctgataaaag ttgctgctaa
     4261 aaattacctt ttctataatc ccataaaaat ttggtttttt gaacttacat atactgaaaa
     4321 tacaggtatt gcctttggtg ctttcaaagg tgcttcactt ttttttggag ttgtatctgt
     4381 tgttgtggct gttattatgc ttattgccag agacaggttt tacaggcagg gtaagctgtc
     4441 cccactgggc gatcttgcgg taaatatgat cataggcgga gcgattggca atatggtgga
     4501 cagagtgcgc ttaggctatg ttgtggatat gttctatatt ccgtttttct ctgtttttaa
     4561 cgttgccgac tcttttgtca cgattggcgc cgtactgctt gccatatact ttttaaggac
     4621 tgaaaaaatt ggagcaaaaa ataccagtaa ccagcaatga tgttaataaa agacttgacc
     4681 ttttcatttg tgaaaaagta ccagaatgga tttcaagaac atttatacag aaatctataa
     4741 aggacgggca gattcttgtt aattcagagc cgaagaagcc ggggtacaga ctcaggcagg
     4801 atgacgttat agtacttgag ttacctgaaa aacccgcacc acatgaggtt actccccagg
     4861 atattcctct tgatatagtc tatgaagacc atgacattat agttatcaat aaagaagccg
     4921 ggatggtggt tcatccaagc ggaaactgct taaaggatac tatggttaac gcccttttat
     4981 ttcactgtcc tgaaatacag agggataaga acgatataag accgggtatt gtacatagac
     5041 ttgataagga tacttcggga cttattgttg tagcgaaaaa tgaagcctct ttcgcagggc
     5101 tttctgacca atttagagac cggaccatca ggaaaagcta tcttgcactc gttaaaggac
     5161 aacttattga aaatgaagga agtatagacg cacctatcgg caggcatccg cagatccgta
     5221 taaaaatggc tgtggtagtg gacggaaaac catctttgac agaatttaaa gttttaagac
     5281 gttttggttc ggcggcaact cttgtttctg ttgtcctaaa aacgggaaga actcatcaga
     5341 tacgtgtcca tttcagttat cttggatttc cgctgatggg agacgaagtt tacggtagag
     5401 ctaatgaaga cagaaaatac gatattttca ggcagatgct tcatgcttac cgtcttgagc
     5461 tgattcatcc tgcaaccggc aggaagatga tattcactgc cccactggca gatgattttt
     5521 gcataggaat taaaaatgtc tataggatgg tggagtagat gtctgttctt gtggttactt
     5581 tttcttcaga taataccgta tactttgata aagccataga gataaggaat ctggtttttg
     5641 aaaacgaaca gatttatcct ccgcagttca aaaaggatag ccttgaagat ctttccgacc
     5701 actttctgct gtttgccaac ggaatcgccg tcggaacatg cagaacaagg ataatcgaag
     5761 aaaactgcat gaggatagag aagtttgcta ttctgaagga ttatagaagt aaggggtatg
     5821 gcatatttat gatctctgag atagaaaaat tttctaaaag caagggatgt agcaggctgg
     5881 tctttttttc gcctaaagct ctggaaaatt actttatacg tttgggattt actccagaag
     5941 aagcttcagc tggctcacaa agtagttatg aatgtaaaaa gatgatgaaa ccagttagat
     6001 aagagaagaa tatgatattc ttctcttttt tttgttttat atacattttc aactctaatt
     6061 aagtttattt accgctaatc ggatgcagaa aggttttgct tatgtattgc gatgtattat
     6121 aattgaaaac gaatgagagg tgattcatgt gttttctgaa aaattaggca taaattataa
     6181 tatcagtggc agcggaaaac ctattgtcct gcttaacggt ataatgatga gtattccaag
     6241 ctggacagaa catgtgaagt atcttgaaaa agatttccag gttataacct acgatatgaa
     6301 ggatcaagga aagtccagat acgttgatta tcagtacgat ataagtatgc atgtgcagga
     6361 tcttaaagac ctgcttacgg aaataggaat taaaaaggct aatctcgtag gtctttcata
     6421 cgggggacag gttgctcaac tttttgccct gaaatatcca gaaatggttg ataagcttat
     6481 tttatccaat accatctcac atgtggataa ctttcttaag tcgattggac aggcatggaa
     6541 gacctctgct gcaacatatg acggtgagtt cttttttgat gtttcattgc cttttatata
     6601 ctcaaggtat ttttatcaga gcaattatga gtggcttatg aacagaagaa agcttttcaa
     6661 ggcaactctt accaagcaat ggtttgatgg ctttataaga ctggcttctt ctaatgaaac
     6721 cttcgacata agagaagaaa taaaaaatat cactgctccg actttactga taggtgcaga
     6781 tgaggatata gcaacaccat accttgaaat gcttaaaatg aatgaaaaaa ttccacaatc
     6841 tcaaataatc tgcatcccta aaacagccca tgcaatgttt ctagaaaaac tcccgctttt
     6901 ttgtgattta gtcaaaggat ttttgaaaga atagaagacg gattcgtctt ctattctttt
     6961 ttcacgagta aaaagtataa aagcagtctt ttacttttcc atacctgagt ataaaagagt
     7021 tccgagttct tttattatgc ttttcttatc ttcggcagac ctgttaaaaa gagtctgtat
     7081 accaagataa tgtgaaattc ctaccaaaaa attcgctacg gctgaatagt cataatcttt
     7141 gacatccttc agatttttga gatagccttt ttcaaagttg tcatagtaat cttttacctt
     7201 gccgttcaca acaaactctg cctctctgac tatctcatag tatataagct tgtcttcaaa
     7261 aaatttcagg aaaagataca ttccctgaag ctcctgttca attctgttta aagaatggtt
     7321 gatgttctga cttatgaaat gccttgtttt agaatttatg atatcaatca gttcagacat
     7381 aaactcttcc ttgctgtcaa agtatatata gaaagtacct acagcaaaac cggctttttt
     7441 agctatatcg taaacattga cgttatagta tccgtactcc ccaaacagtt ccatccctgc
     7501 cgatatcaac ttctccctcg aagttgtttc ctgaacagct tcaagttgct tagcatcaaa
     7561 atcaaagatc ttttcatagt tgtttacctg attgctgaaa ataccgttaa gaaccatctt
     7621 ctccccttga agcaaaatct tattatccaa aactatatgc ttatacaaag atcttattga
     7681 aaggaatctt attccacaag taccgaaaag atactccgcc tccgaaatat cttttttaaa
     7741 gacctcagaa agaccttttt tatagatatc tgccagtttt ttttcgtact cctgaaacct
     7801 gtactgccct tccctatata cacttaccat ccttttaaac ttaacacccg aattaactat
     7861 agcattaaaa agatagtcta gtctctgagt gaccgactct cctttgatat gctcaaggtt
     7921 ttcactgaga tctttgagga agatgtcaag gatctctttg aaaatctctt ccttatcttt
     7981 gaagtacctg taaaaaactc cgttggaaag gtttgcattt ctgcatatct ctgctactga
     8041 gactgtttca taccatcttt ccgaaaatga ctcaagagca gattcgataa gcctgtcttt
     8101 ggttttcatt ctggtatctt ccaagttgct cactcccata caaaagaata cctatagtcc
     8161 ttaaggacta taggtttaca aaatttatta acaatttttt aaacaattgc gaatcttcca
     8221 atataacaga gtgtccgaca tgatcaagca cctcgagcct gccgtttaaa ttcttaagag
     8281 tgttttccgc catatcatgt gtaatcaaca tatccttctt tccaagaata aaaagaacag
     8341 gatttttata cctttcggca agttctgtat aatcataccg tgataaggct cttgcattct
     8401 cgctgaaaca ttcatctttc atgagcaggg catctttaac aacttcagaa agaattttct
     8461 gatcttcaca agccggcata attcctttaa ggctgttcct gagaagagat acgttacctt
     8521 tcatcatctc aaggacagga aagttctctt ccggcgtgac aagaccgttt acaggcgcag
     8581 agtctaccag aaccagctta ctcaccaatt gaggatatct gaaggcaaga gacatactaa
     8641 cagcaccgcc aagtgaatgt cctactacca ctgccttttc tatcttaagc tgatccatga
     8701 agccctttac aaagtcagcg tatgtatcta tgctcacatt atcaattcta tcagattttc
     8761 caaaatttac aagatctggt atataagttg tatatccttc tatactgctt accttttcat
     8821 accatctgtg tgatccgaaa ttaccgtgga tcattactat ggctggaccg cttccttgct
     8881 gccggtaaaa aatctttttc ccgtttacgg taacgtactt cccatcttca gccctacggc
     8941 tccaactctt tcttaccagt aaagcccatg gtctcccaag atacttcata gaaaggagct
     9001 tcagaccgta agcaattccc tgtggaaaga ataagacaaa tactatgaga agtgctccga
     9061 atataagcgt cattgggata tttccgcgcg aaaacaggaa aggaagacct gttattatta
     9121 ttgaacctat cataccgcct tccagagatg ccatacctcc gattacaatc attgacaaaa
     9181 ggtttaatga tgtgttcatt ccaaaatcag gaggagaaat atatcctacc gtatgcgagt
     9241 ataatgcacc cgctattcca ccataaatcg cacttactat gaaagcgttc aattttatcc
     9301 ttgaaggctt aattccatat gctcttgccg caacctcact gtctcttacc atcttatact
     9361 tttcaccgac cggagagtgc aagatgagcg atgcaacata cgttagaacc acaaaaaaca
     9421 caagatttag aagataaata taaaaatcac tttcaaaaaa tcttgggata tttctcatcc
     9481 ctatatgtcc accaaaatgt tctatggcgg caaacagctg ttcaacagca actccaaacg
     9541 ccattgtagc tattgcaaga taaaaaccct tgagtcttaa tgccggcagc cctattaaaa
     9601 atccaagaaa cgctgcaaga actatcgctg caagtatgtt tatataaaaa ggagttccaa
     9661 agtccatagc catagcagct gtagtataag ctcccacagc cataaatgct ccgtgtccaa
     9721 tagaaacttg accggtaaag cccgatacta tattcagtcc agtagcagca atagcatata
     9781 taagcacaag agaaaatatc atcagaagga aggatttgtt tataaataat actcccaata
     9841 gtactcctat tataagtact atcaaagctt ttttcatgtt catacttaca ctctcccttc
     9901 aaacttcttg gcaaaaagac ctgaaggctt aaatgccagc atcagtatta ttataagaag
     9961 tattatcgtg agctgataat ccggggagat gtaaattcca actatttttt caagaatccc
    10021 gagtattata ccacctagaa ttgatccgta aaggcttgta aatcctccga gaacacctgc
    10081 tgtaaaaccg taaagctgca tgtttatcat catagtcgga gttactgtgg ttttaggcgc
    10141 aataagaaca cctacaagag cagaaaggct tatccctata ccccatacca acccgtctat
    10201 catatttgtg tttattccaa ccactttgga accgacctca tcttgtgatc gtgctcttat
    10261 agctattccc actttggtta ctttgaggaa aactgccaac agaatggtta ttccaagcga
    10321 aataactgtt ataagcagat cgttgagcgg aaggataaga agtccatcct caccgagtg
//
LOCUS       DAOSHR010000065        10186 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_5606_length_10186_cov_5.523344, whole genome shotgun sequence.
ACCESSION   DAOSHR010000065 DAOSHR010000000
VERSION     DAOSHR010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 10186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10186
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5606_length_10186_cov_5.523344"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /locus_tag="PLU28_04985"
     CDS             complement(<1..747)
                     /locus_tag="PLU28_04985"
                     /inference="COORDINATES: protein motif:HMM:NF014571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046353 - aminoglycoside
                     3-N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
                     /protein_id="HOG34455.1"
                     /translation="MSTTQEVLQKCLKDLKSLNIYNTPYLTVHTSMKSINEKKLTPQM
                     LLECLRQSISPSSHLIVPTLSYLSTENAGDVFNVRTTPSDVGLFTETVRKLFPQNRSL
                     HPTHSVCVFGNNSEKMCLDHLNDSTPVGRYSPLSINLHNDGLIMMIGCGPAPNTTMHA
                     VEELVNPEYLFLSEKRYSVIDINQKQTVKEYRPHGFKTVIQRYDRLIKLLSTNDYLQG
                     ELFSARVLIIKCSAILKKAVPALEQNSYFFV"
     gene            complement(758..1789)
                     /locus_tag="PLU28_04990"
     CDS             complement(758..1789)
                     /locus_tag="PLU28_04990"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOG34456.1"
                     /translation="MKKINVAIAGYGMAVKTFHLPVLSCIEEIDVKKLYSPSIKNTDG
                     LYSSIEKTANINSLMTPEIDLIVIATPNHTHYELAKLFLENEKNVVVEKPFTVTSSQA
                     QELEELALKKHLLLSVHHNRRWDGPFLTVEKIIKNNTIGTITYFESHFDRFRPQVRDR
                     WKESSIQGAGILYDLGPHLIDQALHLFGMPQKIYADIQMQRKDSKSPDFFEIKLFYPH
                     MNVVLKAGMLVNYPTPHFLLFADKGTFIKYGYDTQEALLNNGLTPCTGKDWGKDKPEN
                     YGLIRYFDGDEEKIETVAGSYTQYYKDIYISLITGKDPQITAKDGRNTIFIIEKAIIS
                     SQEQQILTL"
     gene            complement(1789..2442)
                     /locus_tag="PLU28_04995"
     CDS             complement(1789..2442)
                     /locus_tag="PLU28_04995"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOG34457.1"
                     /translation="MKKNISPQEKDQKVQHITECAEKIFYEKGFEDTSMDYIAMKADV
                     AKGTLYLYFTSKKDLYYAVVLRGLKKLEAMIVQHTQKFKTGFEKTYEMARMYIEFFYS
                     HREYYEFIVEYESHRAKDAEVNPFVRACYKESGKLFDILCEYIAKGKEDKSIKESILP
                     QKDSLVFWIQTVGLLQQLYLKEKLYKNMTDITSSEIIERHIEMIASFLKNGSAGQEE"
     gene            2570..3439
                     /locus_tag="PLU28_05000"
     CDS             2570..3439
                     /locus_tag="PLU28_05000"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOG34458.1"
                     /translation="MFYTRKVLMSDYDDIVDISKDIWGGTDYLPWIFKDWVNDENGAF
                     ICVCDSDKNGKVVGVDKYTILYDHTGWLEGLRVHKNYRGLKLSVILAEKVLEIAKKDL
                     ACGKINKIAFSTHITNVESISLMSKYNFKLEQEQVMLSKSGEKIKTKPPFTIEKWDIS
                     LHEFMELDYLKKRNNLVPLAFVFQSPTEELYQKYKKEDSFVQINGHKGIVVSKSGEQY
                     FIAIEDTFDAIDVFMDCLLAIKTSENYMEPMTFVLPENRQVIEEALKSGYSVYENGKP
                     DYLYYVLKGMPPF"
     gene            complement(3459..4301)
                     /locus_tag="PLU28_05005"
     CDS             complement(3459..4301)
                     /locus_tag="PLU28_05005"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HOG34459.1"
                     /translation="MIKIGAHMAITKGFSKVPEDSFNIGANTFQIFVHSPRVWAVTPI
                     KPNDDVQFKENLKKYCIDPQDILVHSGYLINLASPKDDMWEKSINSMINEMQLCSQLG
                     IKYFNVHPGSHLGEGEEFGINRIAKALDTIFKKTSPDTTVLLENTAQKGGNIGYSFDQ
                     LGKIIKTVQNNSRLGITYDTCHGFDSYYDIRTKDGVRKLISEIDDSMGFDRLRMIHLN
                     DSKFDLGAGKDRHEFIGKGFIGIEGFRTFLSFDEIRKIPLLLETPGNDADHANDIMTV
                     KSLL"
     gene            complement(4298..4999)
                     /locus_tag="PLU28_05010"
     CDS             complement(4298..4999)
                     /locus_tag="PLU28_05010"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IspD/TarI family cytidylyltransferase"
                     /protein_id="HOG34460.1"
                     /translation="MNLSVILAAGKGSRTGLDLPKQFYQINNKTILSLCLEKFEYSVL
                     TDGFIVACHPDYYEKAMEIAGAFKKSRGVVYGGKTRQESVFNSLKYLSKTFVGIDLVA
                     VHDSARPFITSQQIDDLFSFAAQKGSVIPVEEESYTVSLTENERIVKYLDRKQICRHQ
                     TPQIFDFERLFSAYEKNVNCLKHFTDDGSIYMAAGNELFIKYISCNNLKITSMQDVLF
                     AEYMLGHKLNEEEKK"
     gene            5103..6050
                     /locus_tag="PLU28_05015"
     CDS             5103..6050
                     /locus_tag="PLU28_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOG34461.1"
                     /translation="MKVVGVDLGGTEIKIGLVDKENGIEKKISLPTEVSKGTEKVVKN
                     ISDGISTISKGVKIDAIGIGSPGSIDRDEGVVRFAPNFPEWKNFELSKAIGSIVNLPV
                     FVENDANSFALGEWYFGHATGLTDFVCITLGTGIGGGVVSHGILITGKDGIGTELGHV
                     IIEPDGPLCGCGNRGCAESIASASSVARTARELIKRFPESLVLKIAGSIESIQSKHVF
                     AAAQQGDYMAQMIVEKAVDVLARLIGGCIHIFNPEKIIIGGGMSKAGDALILPLRKRV
                     EAYIMVSFKNTYTIHASDLVENAGILGAASSAIYALRGD"
     gene            6052..6750
                     /locus_tag="PLU28_05020"
     CDS             6052..6750
                     /locus_tag="PLU28_05020"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOG34462.1"
                     /translation="MQKTVTFFIIMLNLLIFVMMFIFGGFGAYDSAASLLRFGAQNGI
                     IVASGDWFRTITSMFVHGGFLHLFFNMYALFYFGNMVERIYGKYKYLSVYVISGFVGN
                     LLTQFLMPTAYSVGASGAIFGLIGLLFGQGFRHDTPNMLKPVTGTALLPVIIINVILG
                     FTVPSVNNFAHIGGLAAGFTFGWLTPVTFNKRTWTIWKVVFYVLVAFIALCYVLLLIS
                     NIKYRFFIIPSMGR"
     gene            6755..8248
                     /locus_tag="PLU28_05025"
     CDS             6755..8248
                     /locus_tag="PLU28_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOG34463.1"
                     /translation="MKELYYKVVRDPVYSEVFLSPLEIIISDTPNMQRLRYLSQLVGA
                     EYVYPSATHTRFGHSVGVMHLSGLYAQHLYPYDYSKFRVLRLAGLMHDIGHGPYSHQF
                     DDTVYKTMGYKDGHDEFRTRIIRGKLLEDTLSVYKNSSDPRIKNDFIHDIELTLGIQT
                     NEDNIHANLQEIFEKVIQVYEGENTGSVDFNIVQGPLGADRLDFVLRDSYFAGTRHFG
                     SGATDRLIRGSSITSDKICYSIKVIDDVYTSLFARFMMYKNVYFHKTSRAADQMIQEV
                     LRASYEVLNLKQRVEDPQQFVELTDARIMDEIEILYQMLFEKKNYGMLMDPELIRHAE
                     KLSYAHSVIQRLKKRDLWKVIMEIPFSTTGVDPSLINNSVGQNMLDAMKQKLKDLLAQ
                     EIPVQDKTEITRILEEFETLFKIDTPYKLSLIHPREFLANDVYIKDTNGDILGFDQMY
                     EKYPAYRFMESNLIQIVRVYIKEDRREILKKYNVLPQSTSQKITTRW"
     gene            8252..9139
                     /locus_tag="PLU28_05030"
     CDS             8252..9139
                     /locus_tag="PLU28_05030"
                     /inference="COORDINATES: protein motif:HMM:NF013589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HOG34464.1"
                     /translation="MGKVLSASLYDYRVRLVCCDTTDLVQDITAVHDLSPQSAVVLGR
                     VCTASVLLGSWISENEKVAVVFKGDGPAGEVAAESDFNLNIRGYIQNSKLEIKRNALE
                     KADVASSIGKGFVYVVRDLGLKTPVTSSIKIVSGEVAQDIAYYMNKSEQVPCAVNIGV
                     LVNSQGIAGAGGMMIQILDQHLQDSIISEIEEIVYKMKNISSYIAEKNSLEKILKLFP
                     KAQNIKESNCRFFCGCTKEKSIQALNAFGVGELKEMQKQKTAEIKCKWCSKRYYATTD
                     DIEKIISEKQVSENKNTEA"
     gene            9141..>10186
                     /locus_tag="PLU28_05035"
     CDS             9141..>10186
                     /locus_tag="PLU28_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HOG34465.1"
                     /translation="MLDEFVGKNIIDESIQSLEEKNKLINAVIRKQLVKGSEDGKYYG
                     IPFLVKDNMEVEGTVTSCCSKILENHVSTYTATAVQKLLDQGFSIIGKTNMDEFAMGG
                     ANENSIYGPVRNPHDHQRVSGGSSGGSAASVAAGIAPFALGSDTGGSIREPASFCGIV
                     GYKPSYGMISRYGLIAFGSSLDQIGVLSRSVYDSAKVVDMMKGKDKKDSTSIDYKKDL
                     TARFGRKLEGLRVAVPDIVFSQEIPQEIKEAFSSSLELLKKCGATVIPVSIPELKYVV
                     SVYYIIAPAEASSNLSRFDGVRYGLRNEKDNLIDTYMSTRQDGFGAEVKRRIMMGTFT
                     LSSAYYDAYFSKAS"
BASE COUNT         2993 a         1755 c         2221 g         3217 t
ORIGIN      
        1 aacaaaaaaa tatgaatttt gttcaagtgc cggaacagct tttttaagta tggcggagca
       61 tttaatgatc agcactctgg cagagaaaag ttctccctga aggtaatcat tcgtgctgag
      121 cagttttata agtctgtcat atctctgtat gactgtttta aatccatgtg gacgatactc
      181 ttttactgtt tgtttttgat ttatatctat aacagagtat cttttttcgg ataaaaaaag
      241 atactctgga ttaaccagtt cttcaaccgc atgcatggtt gtattgggag cagggccgca
      301 gcctatcatc attatcaagc cgtcattatg aaggtttatt gataagggag agtatcttcc
      361 tacaggagtt gaatcgttaa gatgatccag acacattttt tcggagttgt ttccgaaaac
      421 acatacggaa tgtgtcggat gcagggaacg attttgaggg aagagtttac gtaccgtttc
      481 ggtaaacagt ccgacatccg aaggggtagt tcttacatta aaaacatctc cggcattttc
      541 tgttgataaa tagcttaagg ttggaactat gagatgagac gagggggata tgctttgccg
      601 caggcattcg aggagcatct gaggtgtaag ttttttttca tttattgatt tcatagatgt
      661 atgaaccgta aggtaaggag tattgtatat atttagactt ttaaggtctt ttaggcattt
      721 ttgaagtact tcctgcgtag ttgacatatt ctctccttta taaggtcagt atttgctgtt
      781 cctgcgaact gatgattgct ttttctataa taaatatggt atttcttccg tccttggctg
      841 taatctgtgg atccttgccg gtaatgagag agatgtatat gtctttataa tactgtgtat
      901 atgaacctgc gacagtttct atcttctcct catctccgtc gaagtaacgg ataagtccgt
      961 aattctcggg tttgtctttt ccccagtctt tgccggtgca tggtgtgaga ccattgttaa
     1021 gaagggcctc ctgtgtgtcg tatccatact ttatgaaagt acccttgtct gcaaataata
     1081 aaaagtgggg tgtaggataa tttacaagca tgccggcttt aagaacaaca ttcatatgag
     1141 ggtaaaacag ttttatttca aaaaaatcag gacttttgga atcttttctc tgcatctgaa
     1201 tgtcagcata gatcttttgc ggcattccga aaagatggag tgcctggtct ataagatgcg
     1261 gtcccagatc atataagatg cctgctccct gaatagagct ttccttccat ctgtctctta
     1321 cctgtggacg gaacctgtca aaatgacttt caaaataggt aattgtacct atagtattat
     1381 tttttattat tttttcaact gtaaggaacg gtccgtccca acgcctgtta tgatgaacag
     1441 agagaagaag gtgttttttc agtgcaagct cttcaagctc ctgtgcctga gaagaagtga
     1501 cggtaaatgg tttttcaaca acaacatttt tttcgttttc gagaaacagc ttggctagtt
     1561 catagtgggt atgattagga gttgcaataa ctattagatc tatttccggg gtcatcagac
     1621 tgtttatatt cgcagttttt tcgatgctgc tgtatagacc atctgtgttt tttatggacg
     1681 ggctgtaaag tttttttaca tcaatctctt caatacagga tagaaccgga agatgaaatg
     1741 ttttgacagc cattccgtat ccggctatcg caacgtttat ttttttcatc attcctcctg
     1801 ccctgcagag ccatttttga gaaaggaagc aatcatttct atgtgtcttt ctattatctc
     1861 ggatgaagtt atatctgtca tatttttata aagcttttct ttaagataaa gctgttgaag
     1921 cagacctact gtctgtatcc agaatacaag tgagtctttt tgtggcagga tgctttcttt
     1981 aatgctttta tcctcttttc cctttgcaat atactcacaa agtatatcaa aaagctttcc
     2041 gctttctttg tagcatgcgc gtacaaatgg attaacctct gcatctttgg ctctgtgtga
     2101 ttcatattcg acaataaatt cgtagtactc tctatgactg taaaaaaact ctatatacat
     2161 acgtgccatc tcgtatgttt tttcaaaacc ggttttaaat ttttgagtgt gttgtactat
     2221 catcgcttcg agttttttaa gtcctctgag aactaccgca tagtacaggt cttttttgct
     2281 tgtgaagtaa agatacagcg ttccctttgc tacatcggcc ttcatggcta tataatccat
     2341 ggaggtatct tcaaagcctt tttcataaaa aattttttcg gcacattctg ttatgtgttg
     2401 aaccttttga tctttctctt gtggggaaat attttttttc atacctttca ggcggtataa
     2461 taggtatatc cgccgttttt cacctgcctt attttaagat gaggcaatac aaaaaaattt
     2521 taacacagtt ctttagattc agtcaatatt agtagatgga ggaaagagta tgttctatac
     2581 aaggaaagtg cttatgagcg attatgatga tatagttgat atttcaaaag atatttgggg
     2641 aggtacggac taccttccat ggattttcaa ggattgggta aatgatgaga acggagcgtt
     2701 catatgtgta tgtgattcgg ataaaaacgg aaaggttgtc ggagtagata agtatacgat
     2761 tctttatgat cataccggct ggcttgaggg tctgagggtt cataaaaatt atagagggct
     2821 taagctgtct gtaatcttgg cagaaaaagt tcttgaaatt gccaaaaaag atcttgcttg
     2881 cggtaaaatt aacaagatag ctttctcaac acatataaca aatgtcgaga gtatttccct
     2941 catgtcgaag tacaacttta aactcgaaca ggaacaggtt atgctttcaa aaagcggaga
     3001 aaaaattaaa accaaacctc catttaccat agaaaaatgg gatataagcc tgcatgagtt
     3061 tatggagctt gactatctta agaaaagaaa taatttagtg ccgctggcct ttgtatttca
     3121 gagtccaaca gaagagcttt atcagaaata caaaaaagaa gacagttttg tacagatcaa
     3181 cggacacaag ggtatagtgg tcagcaaatc gggggaacag tattttatag caattgagga
     3241 tacgtttgat gcaatagatg tttttatgga ctgcctgctg gctatcaaga cttctgaaaa
     3301 ctatatggaa cctatgacct ttgttttacc ggaaaacagg caggttatag aagaagcatt
     3361 aaaatccggt tactccgtat atgaaaacgg aaaacccgat tatctttatt acgttctgaa
     3421 aggcatgcca ccgttttgaa cagttcatga ttctttgatc agaggagtga tttcacggtc
     3481 attatatcgt tggcatggtc ggcatcgtta ccaggagttt ccaataaaag cggtatcttt
     3541 ctgatttcat caaaggaaag aaaggtcctg aatccctcta taccgataaa gccttttcct
     3601 ataaactcat gccggtcttt tcctgctccc aggtcaaact tggagtcgtt taaatgtatc
     3661 attctcagcc tgtcaaaacc catgctgtca tctatttcgg agattagttt ccgcacaccg
     3721 tctttggtgc gtatatcgta gtaagaatca aaaccatggc atgtatcgta ggttattcct
     3781 aaacggctgt tattttggac ggttttgatt atttttccga gctggtcaaa gctgtatccg
     3841 atatttccac ctttctgtgc ggtattttcc aaaaggacgg ttgtatctgg agatgttttt
     3901 ttaaatatag tatcaagtgc ttttgcaatt cggtttattc caaactcttc tccctcaccc
     3961 agatggcttc cgggatggac attgaagtat ttaattccta actgtgagca tagctgcatt
     4021 tcatttatca tggaatttat ggatttttcc cacatatcgt ctttgggaga agcaagattt
     4081 ataaggtaac cgctgtgtac aagaatatcc tgtggatcaa tgcagtattt tttgagattt
     4141 tctttgaact ggacatcatc gttgggtttt ataggggtaa ccgcccacac tctaggtgaa
     4201 tggacgaata tctgaaaagt gttggctccg atgttaaagg aatcttccgg tacctttgaa
     4261 aacccttttg ttatggccat atgggcccct atttttatca tttcttttcc tcctcgttta
     4321 atttatgccc tagcatatac tctgcaaaaa gtacatcctg catggaagtt atttttagat
     4381 tgttacacga aatgtatttt ataaacagct cattgcctgc tgccatgtat atggaaccgt
     4441 catcagtgaa gtgttttaaa cagttcacat ttttttcgta ggcactgaaa agtctttcaa
     4501 aatcaaatat ctgcggtgtc tgatgccggc atatctgttt tctgtccaga tactttacaa
     4561 tcctttcatt ttcagtcagc gatacggtat aactttcttc ttcaacggga ataacagatc
     4621 ctttctgtgc agcaaaggaa aaaaggtcat ctatctgctg tgaggttatg aaaggccttg
     4681 cgctgtcatg aactgccaca agatcaatgc ccacaaaagt cttactaaga tacttcaatg
     4741 agttgaaaac gctttcctgc cttgtctttc ctccatatac aactcctctt gattttttaa
     4801 aagcaccggc gatctccatg gccttttcgt agtaatcagg gtggcaggca acaataaagc
     4861 catctgtcag tacagagtat tcaaacttct ctaaacataa acttaggata gtcttattat
     4921 ttatctgata gaattgtttc ggcaaatcca gtccagttct gcttccttta cctgcggcaa
     4981 gtattactga aagattcata aatttcctcc ggaaaaaatg atgtataata attaacatat
     5041 atagcttttt taagaataat actaaaatta taacatgata aaatgctaag gagtgtggtc
     5101 ccatgaaagt cgttggagta gatctaggcg gaacagagat aaaaattggt cttgtggata
     5161 aggaaaacgg aatagaaaaa aagataagtc ttcccaccga ggtttcaaag ggaacagaaa
     5221 aggttgtaaa aaatatatca gacggtattt ccacaataag caagggtgtg aagatagatg
     5281 ccataggtat aggttctccg ggttcaatag acagggatga gggtgtagtc agatttgccc
     5341 ctaattttcc tgaatggaaa aacttcgagc tatccaaggc gataggcagc atagtgaatc
     5401 ttccggtttt tgtagagaac gatgccaatt catttgctct gggagaatgg tattttgggc
     5461 atgcaaccgg ccttactgac tttgtatgta taactttggg aacaggtata gggggcggag
     5521 ttgttagtca cggcatttta attaccggaa aggacggtat cggaacggaa ctcggtcatg
     5581 ttataattga accagacgga cctttgtgcg gctgtggcaa cagagggtgc gctgagtcaa
     5641 tagcatcagc aagctctgta gctagaactg caagggagct cataaaaagg tttccggaat
     5701 ctttggtctt aaagatcgca ggtagtatag aaagcattca gtcaaagcat gtgtttgcag
     5761 cagcccagca aggtgactat atggcacaga tgatagttga aaaggctgtt gatgttcttg
     5821 ccaggcttat aggaggctgt atacatattt ttaatccaga aaagataatt ataggcggtg
     5881 gaatgagcaa agccggcgat gctcttatac ttccattgag gaaaagagtg gaagcatata
     5941 taatggtaag ttttaaaaac acatacacta ttcatgcatc tgatctggtt gaaaatgccg
     6001 gtattcttgg tgcggcatca tcggccatct atgctttaag gggtgattaa tttgcagaag
     6061 acggtaactt tttttatcat catgctcaat ctcttgattt tcgttatgat gttcatattc
     6121 gggggctttg gggcttatga ttctgcagcc agccttctca gattcggggc acagaacggt
     6181 attattgtag cgagcggtga ctggttcaga acgataacct cgatgtttgt tcacggcggg
     6241 tttcttcatt tgttcttcaa tatgtatgca ttgttttact ttggaaatat ggttgaaagg
     6301 atatacggaa agtataagta cctttctgtt tatgttatat ccgggttcgt cggcaatctt
     6361 ttgacacagt ttcttatgcc tactgcatat tcggtaggtg catcgggagc catttttggt
     6421 ttaatagggc tgttatttgg gcagggtttc aggcatgata cgcccaatat gctcaaacca
     6481 gttaccggaa cagcacttct tccggtcata atcattaacg ttattcttgg ctttacagtt
     6541 cccagtgtaa ataattttgc tcatattgga ggtcttgccg caggctttac ttttggatgg
     6601 ctgacacctg taaccttcaa caagcgcaca tggactatat ggaaagtagt tttttatgtt
     6661 cttgtggcct ttatagctct ttgttatgtt ttgcttttga taagcaatat caagtacagg
     6721 ttttttataa taccttcgat gggaagatga taatatgaaa gaactatact ataaggtagt
     6781 gcgcgatccg gtttattccg aagtttttct ttcgcctctt gagataataa tatctgatac
     6841 tcccaacatg cagaggttaa ggtatctaag ccaactggtg ggggcggagt atgtttatcc
     6901 cagtgctact catacaagat ttgggcattc tgttggagta atgcatcttt caggtcttta
     6961 tgcccagcat ctttatccgt atgattattc aaagttcagg gtgctgagac ttgccggatt
     7021 gatgcacgat ataggacacg ggccttactc gcatcagttt gacgatactg tctataaaac
     7081 tatgggatac aaggacggtc atgatgagtt caggacaaga ataataagag gcaagcttct
     7141 tgaagatact ttatcggttt ataaaaattc tagtgaccca aggataaaaa atgattttat
     7201 tcatgatata gaactgactt taggaataca gacgaacgaa gataatattc atgctaatct
     7261 tcaggagata tttgaaaagg ttatacaagt ctatgaaggg gaaaatacgg gaagtgttga
     7321 tttcaacatt gttcagggac ctcttggggc cgacaggctt gattttgttc tcagagattc
     7381 atactttgct ggaacaaggc actttggttc cggggcaact gacaggctta ttagaggaag
     7441 cagtataact tcagataaga tatgctactc aataaaagtt atagatgatg tttatacttc
     7501 gctattcgca agatttatga tgtataagaa cgtttatttt cataaaacct caagggcagc
     7561 cgatcaaatg atccaagagg ttctcagagc atcttatgag gttcttaacc tcaaacagcg
     7621 tgtagaagat ccccagcagt ttgttgagct tactgatgca aggataatgg atgagattga
     7681 gatactctat cagatgcttt ttgaaaagaa aaattatggc atgctcatgg atccagagct
     7741 tatacgtcat gcagaaaaat tatcttatgc acattctgta atacaaagac ttaaaaaaag
     7801 agatctatgg aaagtaataa tggagattcc tttttccact acaggggttg acccttcgct
     7861 tataaataat tccgtagggc agaatatgct ggatgccatg aagcaaaagc ttaaggatct
     7921 tcttgcacag gaaattcccg ttcaggataa gactgagata accagaatac ttgaagagtt
     7981 cgagactctt tttaagatcg atacacctta taagctctct ttgatacatc ccagggagtt
     8041 tttagccaat gatgtgtata ttaaggacac taacggggat attctggggt ttgaccagat
     8101 gtatgagaaa tatcctgcct atcgttttat ggagagcaat cttattcaga tagtaagagt
     8161 gtacataaaa gaagacaggc gtgagatact taagaagtac aatgttcttc cacagagtac
     8221 ttcccaaaag attacaacaa ggtggtaaaa gatgggaaag gtattaagtg catctttata
     8281 tgattataga gtaaggttgg tatgctgtga cacaacggat cttgtacaag atataactgc
     8341 tgttcacgat ctttctccgc agtctgccgt agtgcttggc agagtatgta cagcatcggt
     8401 tcttcttgga agctggataa gtgagaacga gaaagtggcc gttgttttca aaggagacgg
     8461 gcctgccggt gaagtggcag ccgagtctga ttttaacctt aatatacgtg ggtatataca
     8521 aaattccaag ctggaaataa agagaaatgc tcttgaaaaa gcagatgtgg cttcaagcat
     8581 aggcaaggga tttgtttatg ttgtaaggga tttgggactt aagacccccg ttacatcaag
     8641 cattaagatt gtttctggtg aagttgccca ggatattgca tactatatga acaagtcgga
     8701 acaggttccg tgtgcagtta atattggagt tttggtaaat tctcaaggta tagctggagc
     8761 aggaggaatg atgattcaga ttctggatca gcatcttcag gatagtataa tatctgaaat
     8821 tgaagagata gtctataaaa tgaaaaatat atcgtcttat attgcagaaa aaaattctct
     8881 ggaaaagata ctaaaacttt ttcctaaggc acagaacata aaagagagca actgccgttt
     8941 tttctgcgga tgcacgaaag aaaagtctat tcaggcgctt aatgcttttg gtgttggcga
     9001 gttgaaagag atgcaaaagc agaaaacagc agaaataaaa tgtaagtggt gttcaaagag
     9061 gtattatgct acaacagatg atattgaaaa gataatatct gaaaaacagg tttccgaaaa
     9121 taaaaatacg gaggcgtaaa atgcttgatg agtttgttgg caaaaatata attgatgagt
     9181 ccattcaatc gttggaggaa aagaacaagc ttataaatgc ggttatacgc aagcagcttg
     9241 ttaagggcag tgaagatggt aagtactatg gcataccgtt tttggtaaag gataatatgg
     9301 aggttgaagg aacggtaacc tcttgctgtt caaaaatact tgaaaatcat gtttccacat
     9361 atacagccac agctgttcaa aaacttttgg atcagggttt ttctataatt ggcaaaacca
     9421 atatggatga atttgccatg ggtggtgcca atgaaaattc catatacggt ccggtaagaa
     9481 atccgcatga ccaccaaagg gtatccggcg gaagtagtgg aggttctgca gccagtgttg
     9541 cagctgggat agctcctttt gctctcggat cggatacagg tggttcaatc agagagccgg
     9601 catcgttttg cggaatagtt ggttataaac cttcatacgg tatgatctcg cgctatggac
     9661 tgattgcttt tgggtcatct ctcgaccaga taggggtact ctccagaagt gtttatgaca
     9721 gtgctaaggt tgtagatatg atgaaaggga aggataagaa ggattcgaca agtattgatt
     9781 ataaaaaaga tcttactgct aggtttggca gaaaactcga aggtttgaga gttgcagttc
     9841 ccgatattgt tttttctcag gaaatacccc aagaaataaa agaagctttc agttcatcgt
     9901 tagaactttt gaaaaaatgt ggagcaactg ttattccggt aagtatacct gagcttaaat
     9961 atgtcgtgag cgtatattat ataatagctc cagctgaggc ttcatccaac ctttcaaggt
    10021 ttgatggagt cagatatgga ctcagaaatg aaaaagataa cctgatcgat acttatatga
    10081 gtacaagaca ggatggtttt ggggctgaag taaagagaag aataatgatg ggaaccttca
    10141 cgctgtcatc agcttattac gatgcttatt tttcaaaagc ttcaaa
//
LOCUS       DAOSHR010000066        10163 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_5624_length_10163_cov_5.907301, whole genome shotgun sequence.
ACCESSION   DAOSHR010000066 DAOSHR010000000
VERSION     DAOSHR010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 10163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10163
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5624_length_10163_cov_5.907301"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..801)
                     /locus_tag="PLU28_05040"
     CDS             complement(<1..801)
                     /locus_tag="PLU28_05040"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOG34466.1"
                     /translation="MKDIKLLSEIGLILTGKDDSDKKISMSIEKIGKHFNVSRVYIFL
                     DEDDSSGSSSYEWCNENISSKIGFLKEFKYEAFPGLREIFLRREYLYVNITEENDKNI
                     YQILKNQSIHSLIIFPLFFQDSLRGFIGCDDCLKEHVWSVKSIEFFKTLASLVSNILE
                     VKNYQETLRNSELNTRTFFNTVEDLFFIGDMEGNILHVNTAVIKKLRYTLEQLKRKKI
                     WDLHPKDNQEEIKKLMKSVFLGDRTSRLLPLITSENNIIYVETRIWTAH"
     gene            complement(941..1732)
                     /locus_tag="PLU28_05045"
     CDS             complement(941..1732)
                     /locus_tag="PLU28_05045"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HOG34467.1"
                     /translation="MQQTLIKKVEPTKIEKKVTCEIKNSISRSFTYIFWILILAIAIF
                     FFIRTATTISDGISLYKITVDEKSISSKVEELTSDKDFVSLSKISGYFLSAIVSVEDH
                     RFFNHHGIDFIATTRALITNLINGKTISGGSTITQQLAKNMYFSFDKKYSRKVAELIV
                     ALRLEQEYSKEQILELYCNLIYFGNGYYGIRQAAKGYFNVQPDQLTLEQSIILAGIPK
                     APSTYNVYSMDKRTLERAKTVLYSMIKNGYISYENASKLNFLSTE"
     gene            complement(1890..2510)
                     /locus_tag="PLU28_05050"
     CDS             complement(1890..2510)
                     /locus_tag="PLU28_05050"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOG34468.1"
                     /translation="MKYTNIVFDIDGTLIDTEDIILNSLQKVLKDLHNRCEKKEDLRF
                     ALGITGPKALEILKIYDNGKTLQTWTEYMLNDRKKISLFPGITEALKKLKYMNIKLGL
                     ITSRTRDEYEKQFVPVEISEYFENIVCSEDTLKHKPNGEPMRKYLEISNTENTKTLYI
                     GDSTYDMQCALAEKTDCALALWGCSDPKGIKSTYTLKRPEDIFDIL"
     gene            complement(2545..3519)
                     /locus_tag="PLU28_05055"
     CDS             complement(2545..3519)
                     /locus_tag="PLU28_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34469.1"
                     /translation="MKKDIFVEYLKKRGQSEEDIKNALDVVQNFEDFLNVLGYDIDNI
                     NETIVKNYLKKLVDTSKDDLFRIISLARYGYITGNDILYIAMLVQLDGSEVMDNLYHK
                     LENLSGKEAAEVIFKDVHLPTTGTANTVRAQLMQTVIERLKEKTNEEFRKELFSDCLR
                     DLPDELYEHDKELYMQAGNIEKYLDLKQKEFIEEMYDLRKNNKLFFGQKITDEVIVFL
                     ESSPEITRGKVNGNKLRIVKIPFRTDEWLKEKDPDKKRYYVCHCPWARESLLSENLIP
                     KVSEEFCHCSAGFVKKPFEQIFGRKLKANIIRSALKGDEFCEFEIELL"
     gene            3744..4139
                     /locus_tag="PLU28_05060"
     CDS             3744..4139
                     /locus_tag="PLU28_05060"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOG34470.1"
                     /translation="MTIDLDMTSSIPIYLQIKNHIVISIGKGELKAGEYLPAVRQLAC
                     DNGVNMMTVSKAYSVLRSEGYIEIDRRHGAKISGTKLKNEEFKVRLSCELLLLIASSK
                     ANGLDKNAFIQMCENIFSTMNPQKVQGIV"
     gene            4139..5524
                     /locus_tag="PLU28_05065"
     CDS             4139..5524
                     /locus_tag="PLU28_05065"
                     /inference="COORDINATES: protein motif:HMM:NF022338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HOG34471.1"
                     /translation="MTVIIVLVMIIILWAVYYSSSLLCKYAKKTVLGVTIPDYARNDK
                     KIKKIISAANKEIRIFLFLFFIEIFPILLFYKYPSLIITYLFIWIFFVFFTFNRLISR
                     HRRTLMNIKEENQWVGENLKTISIDTEVTRLKDTVPIKKKNFLIPFLISTLPVLVFVS
                     GSKDLVLLFLSSISFLSTCACYIVYSFLSSGESVEYSHNTSVNLMCNRIRRYWSSCAW
                     FVIAIAESLSFAGLSLFINNKFLFWNIFSIVLSVLVIFGVITVMLVRKKSEDMCKRIL
                     KLEKTSFIIDDDYYWKGGMLFYCNFNDRRVWVDNKTSLGYTCNLASRSGRLFVYLSSV
                     LSIIIIVSLITLFLILDFTVFDMKFQNKEIAISAPIYGYSFSAKDIKDIQLSDKLPQV
                     FKINGAATERYLLGNFDVTGYGSSKMYVYKEKSPFIIIELSGGYVFFGSQNPEKTMEY
                     YERLSKIILEK"
     gene            complement(5560..6087)
                     /gene="cobO"
                     /locus_tag="PLU28_05070"
     CDS             complement(5560..6087)
                     /gene="cobO"
                     /locus_tag="PLU28_05070"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF004637.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOG34472.1"
                     /translation="MGEKMDKGYIHVYTGNGKGKTTAALGLSLRAVCAGKKVYFGQFI
                     KGMKYSELSAAEFLKNFEIHQFGRNCFIFNKPTEEDVLLAKKGLEEIGRIIAQGSYDI
                     VVLDELNIAIYYRLVSIEEVLDVLEKKHPETEVVITGRYAPKELIEKADLVTEMTEIK
                     HYYKQGVQARKGIEN"
     gene            complement(6090..6929)
                     /locus_tag="PLU28_05075"
     CDS             complement(6090..6929)
                     /locus_tag="PLU28_05075"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOG34473.1"
                     /translation="MTIKVLTDNRVRKRGLLAEHGLSLYITHGEQNILFDVGQSNVYC
                     HNAKKIDLDLRKTDIIVLSHGHYDHCMGLPFFPSDGKLPPIYVNRRAFQKKYSSDTIS
                     PQYREVGISFNDKEFSSISSSIVFTGEEHNVKGYESISEDIFLMGNISEITDFENESI
                     RFYTGEDYHRDNMNDEQILIVKKQEGLVVFTGCSHKGIVNIIENIRLKIPETNIYAIV
                     GGMHLENASKTRIEKTIDYLISMNLKYLIPLHCTGIKAMVQMKECFKERCLLLCAGDS
                     IEV"
     gene            complement(6947..7426)
                     /locus_tag="PLU28_05080"
     CDS             complement(6947..7426)
                     /locus_tag="PLU28_05080"
                     /inference="COORDINATES: protein motif:HMM:NF014101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HOG34474.1"
                     /translation="MPRPIKWRKIENIPGQTAFIPVRDENIQIETNVLKFEELEAIRL
                     KDLLGYDQIECAEKMEVSRPTFQRILLSAREKIADSLVNGKGIQVRGGKATQNICRLI
                     CSECGYRWVENYEKFETVLESDIKCPKCESIKVVCEKGCRTGYGFGNCRRGEQRNNF"
     gene            complement(7548..7802)
                     /locus_tag="PLU28_05085"
     CDS             complement(7548..7802)
                     /locus_tag="PLU28_05085"
                     /inference="COORDINATES: protein motif:HMM:NF036294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HOG34475.1"
                     /translation="MPGKDGTGPLGLGYMCNRRFGFRTGGRISGRCYNGRYNREMFSY
                     NSAFLNDQTVERSELEEQLRFYENQIVDLKKRIEQTDKKI"
     gene            complement(7998..8576)
                     /locus_tag="PLU28_05090"
     CDS             complement(7998..8576)
                     /locus_tag="PLU28_05090"
                     /inference="COORDINATES: protein motif:HMM:NF014689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HOG34476.1"
                     /translation="MNIVELLLLALGLSMDAFAVSVSNGMCVKKIKLSDSFIIAFSFG
                     AAQGLMPLLGFYLGQVFIERIKHLDHYIVLILLTFIGVKMILEKLFPSHKEKKQCPVE
                     GLRKSELLLQAVATSIDALAAGVSFAAMQSDILISVGIIAFVTFGVCFAGVRIGKRFG
                     DAVGSWAEVAGGLILILLGLKVFIEHVADHGF"
     gene            complement(8659..9369)
                     /locus_tag="PLU28_05095"
     CDS             complement(8659..9369)
                     /locus_tag="PLU28_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34477.1"
                     /translation="MKLQVIMEYNDEGYLFYFKKYTGAYVRGKTKEEAFSKISQELDS
                     YLKWSCMDENILLDSIEPQVIQEKKSSLKIDDADSDIIFESEKTPLSYSEYSELEKLV
                     LRSAYDFSNMYESIPDKDQTVLKTRKTFYGEVPVTAREMYEHTNNVTSYYAKEIGIDF
                     INDPDIFKNRLNVLQEIEKKEDFLLNKVLLGSYDEFWSVHKALRRFIWHDRIHAKAMY
                     RMALKTFKKDIYNPFFFI"
     gene            complement(9500..9982)
                     /locus_tag="PLU28_05100"
     CDS             complement(9500..9982)
                     /locus_tag="PLU28_05100"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010957027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HOG34478.1"
                     /translation="MEFFDIKVRDMYGRTVTMQEYKGKVLLIVNTATGCGFTPQYEGL
                     ENLYKKYKREGFEILDFPCNQFGGQAPGTAEEINNFCTMKYGVSFRQFEKIDVNGENE
                     SPLFTFLKSKKSSILGTKIKWNFTKFLINRNGEVIARYSPMDTPERIDKDIMRLCSQK
                     "
BASE COUNT         3156 a         1815 c         1537 g         3655 t
ORIGIN      
        1 gtgggcggtc catattcttg tttcaacata tatgatatta ttttccgaag tgataagcgg
       61 aagaagacga gatgttcgat caccaagaaa aacagatttc attaatttct tgatttcttc
      121 ttggttatct ttcggatgaa gatcccaaat tttttttctt tttagctgct ccaaggtata
      181 tcttagtttt ttaataactg ctgtattaac gtgaagtata ttcccctcca tatctcctat
      241 gaaaaaaaga tcttcaacag tgttaaaaaa agttcttgta ttcagttcag aatttctaag
      301 tgtttcctga tagtttttaa cttcaaggat attagataca agtgaagcta gagttttaaa
      361 aaattctatg gatttaacgc tccatacatg ctctttgagg cagtcatcac atcctataaa
      421 tcctctaaga ctgtcctgaa aaaatagtgg aaatattatc agtgaatgta tagattgatt
      481 ttttaatatt tgatagatat ttttatcatt ttcttctgta atatttacat aaagatactc
      541 gcgtctgaga aaaatttctc ttaagcctgg aaatgcttca tatttgaatt ctttaagaaa
      601 accgatcttt gaggatatgt tttcattaca ccactcatag ctgcttgaac ctgacgaatc
      661 atcctcatca agaaatatat aaactctgct tacattaaaa tgttttccga tcttctcaat
      721 agacatgctt atttttttat cggaatcatc ttttcctgta agtattaatc caatttccga
      781 taagagtttt atgtctttca tcacacacct gccattaagt attctatagg tcatgctatt
      841 aattataaca ctcattttta atttttaata cacacaagaa agattatatt aacttttcta
      901 ttatatgtaa aaaaacttcc gtgtatgcaa acggaagttt ttattcagta ctcaaaaaat
      961 ttagctttga agcattttca taagaaatat aaccattttt aatcatagaa taaagaacag
     1021 ttttagctct ttcgagagtt cttttatcca tagagtatac gttgtaggtg cttggtgctt
     1081 tgggaatgcc cgcgagtata atagattgtt caagagtaag ctgatcaggt tgtacattaa
     1141 agtatccctt agctgcctgt cttatgccat aataaccatt gccaaaataa attagattac
     1201 agtataattc aagaatctgt tcttttgaat actcctgttc cagtctcagt gcgacaatca
     1261 gttcagcaac tttccgtgaa tattttttat cgaaagaaaa atacatattt ttagcgagct
     1321 gctgagtgat tgtacttcct ccggaaatag tctttccgtt aataagattt gtaatcaaag
     1381 ctctagttgt tgcaataaaa tcaataccgt gatgattgaa aaatctgtgg tcttcaaccg
     1441 agactattgc tgaaagaaaa tatccagata tttttgaaag acttacaaaa tctttatctg
     1501 aagtgagttc ttctactttt gagcttatac ttttttcatc aacagttatt ttatataaac
     1561 ttattccgtc agatatggtt gtagcagttc ttataaaaaa gaagatggct attgccaata
     1621 ttaaaatcca gaatatgtaa gtgaaacttc tagatatact gttttttatt tcacaggtaa
     1681 cttttttctc tattttggtt ggttcaactt tttttataag tgtttgctgc atctactttt
     1741 ttccccttgt ttttattttt tccttacttc aattttaaac tattcggttt tacaacagta
     1801 tgtttcagtc ttacgtattt cgtacagtat tgtaagatta aaaaatctgc ctttaaaagg
     1861 cagatcaaga gataaatgca attttaagct tacaaaatat caaaaatatc ttccggtctt
     1921 tttaaagtgt aggtactttt aattccttta ggatcactac agccccataa tgcaagagca
     1981 cagtcggttt tttctgccag agcacattgc atatcgtaag tactgtctcc tatataaaga
     2041 gtcttggtgt tttctgtatt cgatatttca agatacttac gcataggttc cccgttaggc
     2101 ttatgtttta gagtatcttc agagcatact atgttttcaa aatactctga gatttcaaca
     2161 ggaacgaact gtttctcgta ctcatctcta gttctggaag taattaatcc aagtttgata
     2221 ttcatatatt ttagtttttt aagagcttct gttattcctg gaaacaagct tattttcttt
     2281 ctatcgttca gcatatattc ggtccatgtt tgtaaagttt ttccgttatc gtatattttc
     2341 aaaatttcaa gagccttagg tcctgttatt ccaagtgcaa atctgagatc ttcctttttt
     2401 tcgcagcggt tgtgcagatc ttttaatact ttctgcaaag agtttaagat aatatcttct
     2461 gtatcaataa gggttccgtc tatatcaaaa acaatattgg tatacttcat ataaatacct
     2521 ccaaatataa tcatcagaag tatattataa cagttctatt tcaaattcgc aaaactcatc
     2581 tcctttgaga gcagatctta tgatattggc tttaagcttt cttccgaaaa tctgttcgaa
     2641 tggttttttt acaaatcctg cactacaatg gcaaaattct tctgaaactt ttggaataag
     2701 attttcagat agtagagact ctcgtgccca aggacagtga cacacatagt atcttttttt
     2761 gtcagggtct ttttctttaa gccattcatc tgttctgaaa ggtattttta caatacgtag
     2821 tttgtttccg tttactttac cgcgggtgat ttcgggagat gactcaagaa atacgataac
     2881 ctcatctgta attttctgtc cgaaaaagag tttattgttt tttctaagat catacatttc
     2941 ttcaataaac tctttttgtt ttaaatccag atacttttca atattgccag cctgcatata
     3001 aagttcttta tcatgctcat aaagttcatc cggaagatct cttagacaat cagaaaataa
     3061 ttcttttctg aactcttcat ttgtcttttc tttaagcctt tctattactg tctgcattaa
     3121 ctgtgcacgc acagtattag cagtgcctgt tgtaggtaaa tgaacatcct tgaatattac
     3181 ttctgcagct tcttttcctg aaagattttc taatttgtgg taaagattat ccataacttc
     3241 acttccgtca agctgcacaa gcattgcaat ataaagaatg tcattgccgg tgatatatcc
     3301 gtacctagct aaagaaataa ttctaaagag gtcatcttta ctggtgtcta cgagtttttt
     3361 cagatagttt tttacaatag tctcattaat attatcaata tcatatccga gaacattaag
     3421 aaaatcttca aaattttgaa caacatctaa agcattttta atatcttctt cagattggcc
     3481 tctctttttc agatactcaa caaaaatatc ttttttcatt ataagccacc tcctgagatt
     3541 ttgttcatta agtagtaagc actaatctct ctataagttc aagttatatt aatatcatta
     3601 taacacagcg agtgaaataa ttcctaagaa gtttggcagg caaaaataaa taattggaaa
     3661 tttggcagtg agattgacat ttgtgtatta tttgtattat aatacataat acaaataata
     3721 cacacttatg tgaggtgttg ggtatgacga tcgatctgga tatgacaagc agtattccaa
     3781 tatatttgca gataaaaaac catatagtta taagcattgg caaaggtgaa ttaaaagcag
     3841 gagagtatct tcctgctgtc agacagcttg catgcgataa cggagttaat atgatgactg
     3901 tttccaaagc ttattcagta ttgagaagtg aaggctatat agaaatagac agaagacatg
     3961 gagcaaaaat atctggtaca aagcttaaaa atgaagagtt taaagtcaga ttaagctgcg
     4021 agcttcttct tcttattgct tcttctaaag caaatggtct tgataagaat gctttcatac
     4081 agatgtgcga aaatatattt agtacaatga atccacaaaa ggtacagggc atagtataat
     4141 gacggtaata atcgtattgg taatgataat tattctgtgg gcggtttact attcatcatc
     4201 attactgtgc aaatatgcta aaaaaacagt gcttggagta actatccctg attatgcaag
     4261 gaatgataaa aaaataaaaa aaattatctc tgctgctaat aaagagataa gaatttttct
     4321 ttttttattt tttatagaaa tctttcccat acttttattt tacaaatatc cgtctcttat
     4381 aataacatat ttatttatat ggatattctt tgtttttttt acatttaaca ggttgataag
     4441 cagacatcgc agaactctta tgaatataaa ggaagaaaat cagtgggttg gtgaaaatct
     4501 aaaaacaatt tcgattgaca cagaagtaac aaggcttaaa gatacagttc ctataaaaaa
     4561 gaagaacttt ctgattcctt ttcttataag tacattacct gttttggtct ttgtttcagg
     4621 cagcaaagat ctggttcttc tttttttgag ttctatatct tttttaagta catgtgcctg
     4681 ttatattgta tattcttttt taagcagcgg tgagtctgtt gaatactccc ataacacttc
     4741 agttaatctc atgtgcaaca ggatcaggag atattggagc agttgtgcgt ggtttgttat
     4801 tgctatagca gaaagtttat cttttgctgg tctaagtctg ttcataaata acaaatttct
     4861 tttttggaat attttttcta tagtattgag tgttttagtg atttttggag tgataacagt
     4921 aatgttggta agaaaaaaat cagaagatat gtgcaaacga atactaaaac ttgaaaaaac
     4981 atcttttata atcgatgacg attattactg gaaaggtgga atgctttttt attgcaattt
     5041 taatgacaga agagtatggg tagacaataa aacaagtttg ggatatacat gcaatctggc
     5101 atccagaagt ggaagacttt ttgtttatct gtcgtctgtt ctgagtatta taattatagt
     5161 aagtctcata accctatttt taattttgga ttttacagta tttgatatga aatttcaaaa
     5221 taaagagata gcaatatcag caccaatata cggatattca tttagtgcaa aagatataaa
     5281 agatatacaa ctttcagaca agcttccgca agtttttaaa ataaacggag ctgcaactga
     5341 acgctatctg cttggcaatt ttgatgttac cggctatggc agttcaaaaa tgtatgtata
     5401 taaagaaaaa tccccattca ttataataga attaagtgga ggatatgtat ttttcggctc
     5461 acagaatcct gaaaagacta tggaatacta tgaacgttta tctaagatca ttttggaaaa
     5521 ataaaatacc cggcagatta aatcttccgg gtaaacttat cagttttcga tgcccttcct
     5581 tgcttgtact ccctgtttat aataatgctt tatttctgtc atttcagtaa caaggtcggc
     5641 tttttcaatg agttctttag gagcatatct tccggttatt accacctctg tttccggatg
     5701 ttttttttct aaaacatcca gtacttcttc gatagaaacc agtctgtagt aaattgctat
     5761 attcagctca tcaagaacaa ctatatcgta acttccttgt gcaataattc tgccgatctc
     5821 ttcaagccct tttttcgcaa gtagcacatc ttcttccgtg ggtttgttaa agataaaaca
     5881 gtttcttcca aattgatgga tttcaaagtt cttaaggaac tctgccgcag acagctcgct
     5941 atacttcatt ccttttataa actgtccgaa ataaactttt ttcccggcac atacagctct
     6001 caaagataga cccagagcgg ccgtggtttt tcctttaccg tttccggtat aaacgtgaat
     6061 ataaccttta tccatttttt cccccatgat tatacttcta tagagtctcc cgcacataat
     6121 aacaaacatc tttctttaaa acactctttc atttgaacca ttgccttaat tccagtgcag
     6181 tgcaaaggta tcaggtattt aagattcata cttataagat aatcaatggt tttttcgatt
     6241 ctagttttac ttgcattttc aagatgcatt cctccaacta ttgcatagat atttgtttca
     6301 ggaattttta gtctaatgtt ttctataata ttaacaattc ctttatgact gcatccggta
     6361 aagacaacaa gaccttcctg ttttttcacg ataagtatct gctcatcatt catgttatcg
     6421 cgatgatagt cctcgccggt atagaatctt atagattcgt tttcaaaatc agttatttct
     6481 gagatgttac ccataaggaa gatatcctcc gatatgcttt catagccttt cacgttatgt
     6541 tcttcccctg tgaatacaat acttgaagat atgctgctga actctttatc gttaaaagag
     6601 ataccaactt ccctatactg agggcttatg gtgtcagagg agtacttctt ctgaaaggct
     6661 cttcgattta catatatagg tggaagcttc ccatcagaag gaaagaaagg taatcccatg
     6721 cagtgatcat aatgcccgtg actaagaact ataatatcgg tttttcttaa atctagatct
     6781 attttcttgg cattatggca gtaaacatta ctctgcccta catcaaataa aatattttgc
     6841 tctccgtgag taatatatag agaaagacca tgctctgcaa gtagtccacg ttttcttacc
     6901 cgattgtcag ttaatacttt gattgtcata ttttcatctc caaagcttaa aaattgtttc
     6961 tttgttctcc tcttctgcaa tttccaaaac catagcctgt acgacatcct ttttcacaaa
     7021 ctactttaat tgattcgcat ttggggcatt taatatctga ttcaagaact gtttcaaact
     7081 tttcataatt ttctacccat ctatacccac attcagaaca gatcagccga cagatatttt
     7141 gagttgcctt tcccccgcgt acctgaattc cttttccatt gacaagacta tcggctattt
     7201 tctcccgtgc gctcagaagt attctttgaa aggtagggcg tgaaacttcc attttttcag
     7261 cgcattctat ctgatcatag cctaatagat ctttcaatcg tatagcctca agctcttcaa
     7321 attttaacac attagtttca atctgaatat tctcatcccg aacaggtata aaagcggttt
     7381 gtcccgggat gttttcaatt tttctccatt taatgggtct aggcatactc cacctcattg
     7441 ctttgagttt caataatgca ttactactaa cgatagtatc actgtaattg attcatgtca
     7501 acattcaaaa aagaccaaag acaagatctt tggtctttta ggtaacatca gattttttta
     7561 tctgtttgct caatacgttt cttaagatcg acaatctgat tttcataaaa tcttaactgt
     7621 tcttcaagct cacttctctc aacggtctga tcatttaaga aagcagagtt gtaagaaaac
     7681 atttcgcgat tatatcgacc gttataacac cttcctgata ttcttccacc tgtcctgaat
     7741 ccaaatcttc tgttgcacat gtatcctaac cctaaaggtc ctgtaccgtc ttttcctggc
     7801 attttatcac ctccataaat aattattgac atatgctcat aattatttta tcataattat
     7861 gagcatatgt caataataaa ataagttttt gtataagtgt gtttgaaatt gattactcca
     7921 aaataataat cagtttattt ttctttgaaa aataatcagt ataaaaaaag tgcccatcta
     7981 agtggacacc ttacttttta aaatccatga tctgctacat gttctatgaa tactttcaat
     8041 cccagaagta taaggataag tcctccagct acttctgccc atgagccgac agcgtctcca
     8101 aagcgttttc ctattcgtac tccggcaaaa caaacaccaa acgttacgaa tgcaattatt
     8161 cctacagaaa taagtatatc cgactgcatt gcggcaaaac tcactcctgc tgctagcgca
     8221 tcaatacttg tggcaacagc ctgaagcaac agttctgatt ttcttaaccc ttcaaccgga
     8281 cattgttttt tctctttgtg agaaggaaat agtttttcca gaatcatttt tacaccaata
     8341 aaggtaagga gtatcagcac aatataatgg tcaagatgtt ttattctttc aataaatacc
     8401 tgtccaagat agaatcctag taaaggcatc aatccctggg ccgctccgaa agaaaaagca
     8461 ataataaagg agtcggaaag ctttatcttt ttaacgcaca ttccgttact gacagaaacc
     8521 gcaaatgcat ccattgaaag tcctaaagcc aggagaagaa gttcaactat attcataaag
     8581 tcctccggta tgcaaaataa tcaggcagtt tctattataa cgcataatat acagtttccc
     8641 aaaaatgtaa cactatgatc aaataaagaa aaacggatta taaatatctt ttttgaaggt
     8701 ttttaatgcc atacggtaca ttgcttttgc atgtattctg tcgtgccata tgaatctcct
     8761 cagagctttg tgaacagacc aaaactcatc ataacttcca agcagaactt tattcagaag
     8821 aaaatcttct tttttttcta tttcttgcaa tacattcaat ctgtttttaa agatgtcagg
     8881 atcgtttata aaatctattc ctatttcctt ggcgtagtaa cttgtcacgt tatttgtatg
     8941 ttcatacatt tctcttgctg taaccggaac ctctccataa aaagtttttc ttgtcttaag
     9001 aactgtttga tctttatccg gaatactttc atacatattt gaaaaatcat atgcagagcg
     9061 cagaacaagt ttttccaatt cggagtattc tgaataagaa agcggtgttt tttcagattc
     9121 aaatattata tcagaatcag catcatcaat tttgagagag cttttttttt cttgaattac
     9181 ctgaggttct atagaatcta gcaagatatt ttcatccatg cagctccatt ttagataaga
     9241 gtcaagctcc tgcgaaattt ttgaaaatgc ttcctctttg gttttcccac gtacatatgc
     9301 accagtatac tttttaaaat agaaaagata cccttcatca ttatactcca ttataacctg
     9361 aagcttcatg acttcccccc ttgaacttag ctatagaatg atctgactta tataattata
     9421 cattattatg aagaacaata aagcagaaaa acgatacatt gtgtttttaa tgataaataa
     9481 aaacttatga cctgacctat tatttttgac tgcataatct cataatatct ttatcaattc
     9541 tttcgggcgt atccatagga ctatatctgg ctataacctc tccatttcta tttataagaa
     9601 atttggtgaa gttccacttg attttggttc ctaaaatact actttttttg cttttaagga
     9661 atgtaaacaa aggagactcg ttttcgccgt ttacatcaat tttttcaaac tgtctaaaag
     9721 atactccgta cttcatagtg caaaaattat ttatttcttc tgctgttcca ggtgcctgtc
     9781 cgccgaattg attacaagga aaatcaagta tttcaaatcc ttcacgtttg tattttttat
     9841 ataaattttc aagaccttca tactgtggtg taaaaccaca gcctgtcgct gtgtttacta
     9901 ttagcaatac ctttccctta tactcttgca ttgtcactgt tctaccgtac atatccctta
     9961 cctttatgtc aaagaattcc atatatacta cctcctcaat ataattaatt acttacaatt
    10021 aaattgtatg caattttaat taaaatgtca aggcctttgc ttgaaaaata aatatataaa
    10081 ttgtaaaatc taaatactta tattctgtga ggtggactat gaatgagaat gagtatttaa
    10141 aactagacaa tcagatttgt ttt
//
LOCUS       DAOSHR010000067        10106 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_5670_length_10106_cov_6.931350, whole genome shotgun sequence.
ACCESSION   DAOSHR010000067 DAOSHR010000000
VERSION     DAOSHR010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 10106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10106
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5670_length_10106_cov_6.931350"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="PLU28_05105"
     CDS             <1..460
                     /locus_tag="PLU28_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HOG34479.1"
                     /translation="EFSLFSRKLYLSPILDMYNGEIISYNISERPHLKQVMDMLNKAL
                     DKISDGTNLIFHSDQGWQYQHKMYQHRLKEKGIRQSMSMKGNCLDNAVMENFFGILKS
                     ELLYLREFESFEEFREELEKYIYYYNHHRIKGKLKGLSPVQYRTQSLLIA"
     gene            complement(538..1836)
                     /locus_tag="PLU28_05110"
     CDS             complement(538..1836)
                     /locus_tag="PLU28_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34480.1"
                     /translation="MKKLLLLFTMILSISIAYSSVLKLVPEDSKAVFVMKNVSATYDQ
                     MKTVPTIGKFLGEPFSAEITFSSYIEMYLNTLDIKPDAFFKSLNTDMAFFIQEPKDSY
                     YPFGIVFGPVDKVQDFTKAMEKILEITKESNISLKSYNKKIDGKDYVIFIQDDKAYAT
                     LKTGLDTSLVKISNGFFFNVNASDLKTTGSVYIKDGFLVGTASGKINEKEISPAYVKK
                     PQDYKFLGTVFGVSGFVPKDFKVFGDIVNIIVDYSIIEKVLMSSSGFELNGNLDINLK
                     NTGMLDIKSINYAKINSKSSIDQLLPVFKDNNIKYEKTSSNAVKIFISQQNQSSSSSQ
                     PKQALPEQIFYLWKDGDNFFVSQTTANDINTMLKTSKNLSDNALFKILNSKVPFGNLA
                     NLFLDISKLFEQYGEKGEFGLLFSVNHTSSGNIKADFILK"
     gene            1997..2407
                     /gene="yajC"
                     /locus_tag="PLU28_05115"
     CDS             1997..2407
                     /gene="yajC"
                     /locus_tag="PLU28_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOG34481.1"
                     /translation="MLEKFIDLINFGSTDGTAAPAASGDPAAQGAGGFLGSWLFIIVI
                     FVLFWLLLILPQRRQKKKHKEMMSKLEKGDKIVNQAGIIGKIVSISNDKIRIRTGDNT
                     QIDITKGSIAAVLSKASSEEEKEEKEESKDKEEK"
     gene            2441..3862
                     /gene="secD"
                     /locus_tag="PLU28_05120"
     CDS             2441..3862
                     /gene="secD"
                     /locus_tag="PLU28_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HOG34482.1"
                     /translation="MKPKTLRLIFTLVILIVAVIPLIFSDRSEPKYMGLQKVLPNVKL
                     GLDIQGGSLLEYSLKVEGTNLKPQEVIDNVVYVLRKRLDAAGYNEATVAKVVSGGEIR
                     VRVEIPGILDTTQAEKLVGSRGKLYFAEVVDTTVSDIKPELSRNRFITVNGQEVGIYE
                     YVQDMNDKLSWYRVKKVFEFGKEPFEVNGSDIIDARASINTQKGGFLVNLRFSSEGAK
                     KFELATGNLVNKRLAIVLDDKVIIAPNVNSRISGGEAVIENIQTIEEAQNSAALIKSG
                     NMPVDLVKFQENTLGPTLGKDIVETVVFAGLLGCGIVLVIIVLLYGWTGIVADIALIY
                     NIVLIIGYMALTKSILTLPGIAGVILAFGTGIDGNVIINERIKEELRLGRPVLTAVGK
                     AFDVSFWAIFDANITSIISCIVLYYFSTGTIRGFAITTIIGLIGSMFTNLVVSRLMLE
                     SSYKLINVKKYYKGNVPEVKEAK"
     gene            3864..4760
                     /gene="secF"
                     /locus_tag="PLU28_05125"
     CDS             3864..4760
                     /gene="secF"
                     /locus_tag="PLU28_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HOG34483.1"
                     /translation="MRNIDFVGKRRIFIAISAVLVLAFVIISFTKGFDVGVEYSGGTE
                     LTFSINEKMSSSEIRDNLAKVKPDYVSAKIVELKSPAEKQDRHFYQVVVKDSLPTTEE
                     KETFIGSLQTAFDGKDLQKEGLNDVSGAAAVELKSFAWYAVILVIISLLLYITLRFKF
                     AFAAGAILALTHDILITLGCYSLFNIEMNAAALASLLTLAGYGLNDTVVIFDRIRENS
                     KKYRGMPIDQIVNKSMNEMFTRTIITALTTFIVVFTMLVWGGKAIAPFAFGMTIGTAV
                     SAYSTIYIASAVVIAVGKKKKA"
     gene            4851..5462
                     /locus_tag="PLU28_05130"
     CDS             4851..5462
                     /locus_tag="PLU28_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34484.1"
                     /translation="MEYKFSKDLKQLIKEKEIDIKKISESIKTTEYTVNKIIENDLSD
                     FSYIQLKDIAQRLSVILKENINVQGVYDEQEDKKVTLKERFLSGGKKFSMIIYYLVIS
                     LFLLGNALMIVVLAKEILTYKALINDQNYSAEIINNSDFPLKVKDTVIDKGGKGFAEF
                     TFGEKITINDNKGEVILKTPFEIYSIKLENFEVILKDGNNKGS"
     gene            5440..5997
                     /locus_tag="PLU28_05135"
     CDS             5440..5997
                     /locus_tag="PLU28_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4416 family protein"
                     /protein_id="HOG34485.1"
                     /translation="MGITKAPDPVNYTAHIFTAGDSDYWLYEKGLKEQLEKRIGKIDY
                     ISGPLDFQKYTFFYNEEMGENIKINARIVSFQELRPQYYLADAKIITNQIEEAFSYDG
                     KRKVNIDIGFVHHTQFVLASTKHWGNRIYIRRGIYAEITLMYVYGKWTALEYSYENFK
                     GQEYQQEIQKVRELYMQKRKNGYDK"
     gene            5906..7330
                     /locus_tag="PLU28_05140"
     CDS             5906..7330
                     /locus_tag="PLU28_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34486.1"
                     /translation="MKTLKVRNISRKYKKFGNFICRKEKTVMINRKKLLLLLSCFIFV
                     SYVFSSARLIFRNDPQFYYTYLKTYPLVLPLRYSQDEDDSTKLYLKLPFGRSEKVAGN
                     YELASGWYLPGTPTDSIFRRNYLELPGIFSLGFSTSYSNNFYASFNVDIPVGDTSFFR
                     YGYENIFEMKDMTHIGLDFPKNSYLSYSTDTIDFSFGRNTISYGPLKYNLLLSDSAPY
                     YDNLSMRVKSGNLNYSFSALSSIPMLSKQEYDQQFSFGNFYAYRNTFVNRLDLSFKNF
                     DFSVSMLNHTGGRLADIRDMFLFSDNGLFSADSRYRGFFEIYTAYCFNYSNFKNSFAA
                     GISKTIDAGDVLIRACGELYHVQDGIYDHEVPLKKLYYRTISVSNNPGSRNFPDTPFG
                     FFYGENSNIFSAEAVIAWEDGYIEVVKDFGNNSYGKVEHFLARINAKMPFADLNILLY
                     DTKNADKDYFGFSFFYILELGVII"
     gene            7327..8481
                     /locus_tag="PLU28_05145"
     CDS             7327..8481
                     /locus_tag="PLU28_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34487.1"
                     /translation="MKYPILVLSIVLSVTLVFSAIVTDKTDLYRSVFYGGYTSNIYPR
                     PSGEDFADKSTFGYDIYIYDKDKYYLLFKAYPFWMIAGKYPTDWGDLERGVYLSDSLP
                     YVKGITSGYENEKLRVIFGIYTFDAALSPQELIRQKDQTIDSNSDRRPLTIPLSGYGD
                     PFKTLIIHRLEVRPFSGIMIAVNELNLIGGKMVDIVDVNPFGILHNTFGEGFSNTMLG
                     VDFSAVIPYGMSVYGQFAMDDFLVPQTESGSQAYKPTAFAYGAGLRKVFKEHNLYISP
                     KIEFYKIYTWMYNRWQPLLKFTGRFGGIDIPMGFDYGNDMQGMLIGADIFTDEKMKIF
                     LGAEYYQKGSVDLNTSYSDNEKKKLTQFWCDLFPPVTDFFALSLKIDISY"
     gene            8494..9588
                     /gene="wecB"
                     /locus_tag="PLU28_05150"
     CDS             8494..9588
                     /gene="wecB"
                     /locus_tag="PLU28_05150"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HOG34488.1"
                     /translation="MKIALILGTRPEAIKMAPVYFELEKRGEQADIISTGQHRQMLDQ
                     ALQIFDIKPRFDLHVMTDRQSLSLLTQKIIAGLQELFDREKYDIVLVQGDTTTSFCGA
                     LVSFYNKIPVGHIEAGLRTNSIYDPFPEEINRRLTSKIASMNFAATSVSADNLLKEGI
                     TEKSIFITGNTVIDSIKWIQKNKQFDTNAIRQKYNVANGKYILMTLHRRENYGRPMEE
                     IFRAVKKFLQYNPEYKLIFPVHMSPDVREYVYKNMEGVKNVILTQPVDYIDFIALMSE
                     SSFILTDSGGIQEEAPTFGIPTVVLRNTTERTEALSAGTAILAGTYENEILACLELMT
                     TEKCLEMAKIKNPYGDGFASQKIVDILQSY"
     gene            9761..9889
                     /locus_tag="PLU28_05155"
     CDS             9761..9889
                     /locus_tag="PLU28_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34489.1"
                     /translation="MKKTYESDTQVIRDNDIYSAEELTEKIKIEKLADVLLYIRQF"
BASE COUNT         3402 a         1687 c         1996 g         3021 t
ORIGIN      
        1 agagttctct ctctttagca ggaagcttta tttatctcca atcttggata tgtataatgg
       61 tgagatcatc agctataata tcagtgaacg tccgcatctg aaacaggtta tggatatgct
      121 caacaaggca ttagataaga tttcggatgg tactaatctg atttttcact ccgatcaagg
      181 gtggcagtac cagcacaaaa tgtatcagca ccgcctaaaa gaaaaaggta tccgccagag
      241 tatgtccatg aaaggcaact gtctggataa tgctgtaatg gagaacttct ttggcatact
      301 taaatctgag ctactgtatc ttcgggagtt tgaaagtttt gaagagttcc gcgaggaact
      361 tgaaaaatac atatactact acaatcatca taggatcaag ggcaagttaa aaggactgag
      421 ccctgttcaa tacagaactc agtccttgtt aatagcttaa tgtactacca atttgtgtct
      481 aactttttgg ggtcagttca aaagatccgg tttttgctaa aaataagatg tagattatta
      541 ttttaaaata aagtctgctt ttatatttcc tgaactggta tgatttacgg aaaataaaag
      601 tccaaactcg cctttttccc cgtactgttc aaaaagcttg gaaatatcta aaaataagtt
      661 tgcaagattt ccaaaaggca ctttagaatt gagaatctta aaaagagcat tatcagaaag
      721 atttttagag gttttcaaca ttgtgttgat atcattagcg gtagtctggg aaacaaaaaa
      781 gttatctccg tccttccata gataaaatat ctgctcaggc aaagcctgct tgggctggga
      841 actagaagaa gattgattct gctgggagat aaaaatcttt accgcattgg atgaagtttt
      901 ttcatactta atattattat ctttgaaaac cggaagcaac tggtcgatag aactctttga
      961 atttattttc gcataattta tactttttat atcaagcatt ccagtatttt tgagatttat
     1021 atctaaattc ccattcaact caaagcctga tgaagacata agaacttttt caataatgct
     1081 gtaatcgaca ataatattaa caatatcgcc aaatacttta aaatccttgg gaacaaatcc
     1141 ggatactccg aaaacggttc ccaggaactt gtaatcctga ggttttttta cataagccgg
     1201 actaatttct ttttcgttta tctttccaga agcagttcct acaaggaagc catctttgat
     1261 atatactgat cctgtagttt taagatcact tgcattcaca ttaaagaaaa aaccgtttga
     1321 tatttttact aaagaagtgt ctaaacctgt ttttaatgtt gcataggctt tatcgtcctg
     1381 aataaaaatg acataatcct tgccatctat tttcttgtta taacttttca aggatatgtt
     1441 cgattccttt gttatctcaa gtatcttttc catggccttg gtaaaatcct gtaccttgtc
     1501 cacaggcccg aaaactattc cgaacggata gtaagaatct tttggttctt gtataaaaaa
     1561 agccatatca gtatttaaag atttaaaaaa agcgtccggc ttaatatcca gagtgttcag
     1621 gtacatctct atgtagcttg aaaaggttat ttctgcactg aaaggttcac caaggaactt
     1681 accgatagtc ggaactgttt tcatctgatc ataagtagcg ctgacatttt tcatgacaaa
     1741 aactgccttg gaatcttcag gaaccagttt caggacactg gagtaggcaa ttgatattga
     1801 aagtatcatg gtgaaaagca gtaacagttt cttcacattt atccctccaa aaaaagtctt
     1861 tttgaagacc gtattattat tattttatca taaacaaaga tgcgttaatc aaaatgtatt
     1921 gtaaaataaa aaacttgata atacactaaa tttagagtat aatctatagg aaaaaaccat
     1981 tttctggagg tgaatcatgt tagaaaagtt tattgatctt ataaactttg gaagtacaga
     2041 cggaacagcg gcaccagcgg catcggggga ccctgcggca caaggagccg gtggcttttt
     2101 gggatcatgg ctgtttataa tagtcatttt cgttcttttt tggctgcttt taatccttcc
     2161 gcagagaaga cagaaaaaga aacacaaaga aatgatgagc aagcttgaga aaggtgacaa
     2221 gatagttaac caggcaggta taatcgggaa gatagtttca ataagcaatg ataaaattag
     2281 gataagaacc ggcgacaaca cacagataga cataacaaag ggatcaattg cagctgttct
     2341 ctccaaggct tcatccgaag aagaaaaaga agaaaaggaa gagtcaaagg ataaggaaga
     2401 aaaataatct ttcattttta tgtaaggggg tatttttatc gtgaaaccaa agacacttag
     2461 gttgatattt actttggtta tccttattgt tgctgtgatt cctctgattt tcagcgacag
     2521 atcggaaccc aagtatatgg gactgcagaa ggttttgcca aacgttaaac tcggcttgga
     2581 tattcaggga ggaagccttc ttgaatactc tcttaaagtc gaaggaacca acctcaagcc
     2641 tcaggaagtc atagacaacg tcgtatatgt cctgagaaaa aggcttgatg ctgccggtta
     2701 taatgaagcg acagttgcta aggtggtaag cggcggagaa ataagggtaa gagttgaaat
     2761 tcctggtatc cttgatacaa ctcaggctga aaagcttgtt ggcagcagag gaaagcttta
     2821 ttttgccgaa gtagtcgata ctacagtatc ggatattaaa ccggaacttt caagaaacag
     2881 attcataacc gtaaacggac aagaagtcgg tatatatgag tatgttcagg atatgaatga
     2941 taaattgtcc tggtacagag tcaaaaaggt atttgagttc ggtaaggagc cttttgaagt
     3001 caatggttct gatatcattg atgccagggc aagtataaat acccagaaag gcggatttct
     3061 tgttaatctg agattcagtt ccgagggagc gaaaaagttt gagcttgcca cggggaatct
     3121 tgttaacaaa agacttgcaa tagtacttga cgataaagta ataattgcgc ctaatgtgaa
     3181 cagcagaata tccggcggtg aagcagtaat tgaaaatatt cagaccatag aagaggctca
     3241 gaacagtgca gcattaataa aatccggaaa tatgcctgtt gacctagtga agttccagga
     3301 aaatacatta gggcctaccc tcggaaaaga tattgtagaa actgtagtat ttgcaggact
     3361 tttgggatgt ggaatagtac ttgttattat tgtgcttctc tacggatgga ctggaattgt
     3421 tgcagatata gcccttattt ataatatagt acttataata ggttatatgg cacttacaaa
     3481 atccatactt acccttcccg gtattgcagg tgtaatactt gctttcggaa ctggtattga
     3541 cgggaacgta attataaatg aaagaataaa agaagagctc aggctaggaa gacctgttct
     3601 tacagcagtg ggaaaggctt ttgatgtttc cttctgggct atatttgatg ccaacattac
     3661 ttccattata tcttgtatag ttctttatta cttctctaca ggtacaataa gaggctttgc
     3721 cataacaact ataataggtc ttatcggaag tatgtttacc aaccttgttg taagcagact
     3781 tatgcttgaa tcttcgtata aactaataaa cgtaaaaaaa tactacaagg gtaatgttcc
     3841 agaagtaaag gaggctaaat aagatgcgaa atatagattt tgtcgggaaa agacgtatat
     3901 ttattgccat atcggcagtc ttagtattag cctttgttat aataagtttt acaaaaggtt
     3961 ttgatgtagg agtagaatac agtggaggaa cagagcttac attcagtata aatgaaaaga
     4021 tgagttcttc tgaaataagg gataacctcg ccaaggtaaa accggattat gtaagtgcca
     4081 agattgttga actgaaatca cctgctgaaa aacaggacag gcatttttac caggtagttg
     4141 taaaggattc gcttcctaca acagaagaaa aagaaacctt tattggcagt cttcagactg
     4201 cttttgacgg aaaggatctc cagaaagaag gacttaatga tgtttcaggc gcagcagctg
     4261 ttgaacttaa atcttttgct tggtatgcag taatactggt tataatatcc ctgctccttt
     4321 atataacttt aagattcaaa tttgcatttg ctgccggcgc aattttagcc cttacacacg
     4381 atatacttat tactctgggc tgttactcac ttttcaatat tgagatgaat gccgcagctc
     4441 ttgcgtcgct tcttaccctt gccggttacg gtcttaacga tactgttgta atatttgaca
     4501 gaataaggga aaactccaag aagtacagag ggatgcctat tgaccaaata gttaacaaga
     4561 gtatgaacga gatgtttaca agaacaataa tcacagcttt aacaacattt atcgttgttt
     4621 ttaccatgct ggtttggggt ggcaaggcta tagctccgtt tgcatttggt atgacgatag
     4681 gaacggctgt cagtgcatat tctacaatat atattgcatc ggcagtggtt attgcggtag
     4741 gaaaaaagaa aaaggcttaa aaatacttat aaatgaggaa cgggcaaccg ttcctttttt
     4801 tacattaata tgctaaaatt aaagtatact ttagaaaagg ggaaaagact gtggagtata
     4861 aattcagcaa agatttaaaa caactcataa aagaaaaaga gatagatata aagaaaattt
     4921 ccgagagcat aaaaacaacc gagtatacag ttaataagat catagaaaac gatctttcag
     4981 atttttcata catacagctt aaagatattg cccagagact aagtgttatt ttaaaagaaa
     5041 atattaatgt gcagggagtt tatgacgaac aggaagataa aaaagttaca ttaaaggaaa
     5101 gatttttatc cggaggtaag aaattttcca tgatcatata ctatcttgtc atatcattat
     5161 ttttattagg aaatgcttta atgatagtgg ttcttgcaaa agaaattctt acgtataaag
     5221 cactcataaa tgatcaaaac tatagtgccg aaataataaa taactctgac tttcccctta
     5281 aagtaaaaga tacagttata gacaagggag gaaaaggatt tgcagaattt acctttggag
     5341 aaaagataac catcaacgat aataaaggag aagtcatact taaaactcct tttgagatat
     5401 acagcattaa acttgaaaac ttcgaggtga tattaaaaga tgggaataac aaaggctcct
     5461 gatccggtaa actatacggc tcatatattt actgctggag acagtgatta ttggctttat
     5521 gaaaaaggtt tgaaagaaca gttagaaaaa agaatcggga agatagatta tatttccgga
     5581 ccgcttgatt ttcagaaata cacctttttt tataacgaag aaatgggtga aaatataaaa
     5641 ataaatgcca gaatagttag ttttcaggaa ttaaggccgc agtactatct tgctgatgca
     5701 aaaataatta ccaatcagat tgaagaagca ttttcttatg atggtaaaag aaaagtcaat
     5761 atcgacatag gatttgtaca tcacacccag tttgtacttg ccagtaccaa acattgggga
     5821 aaccggatat atatacgtag aggtatatat gctgaaataa ctcttatgta tgtctatggc
     5881 aaatggactg ctctggagta ctcttatgaa aactttaaag gtcaggaata tcagcaggaa
     5941 atacaaaaag ttcgggaact ttatatgcag aaaagaaaaa acggttatga taaatagaaa
     6001 aaagcttttg cttttgcttt cttgttttat ttttgtttca tacgtttttt catccgcaag
     6061 acttattttc agaaatgatc cacaattcta ttatacctat ctgaaaacat atccgttggt
     6121 tcttccgctc agatattctc aggatgagga cgatagtacc aaactgtatc ttaaacttcc
     6181 ttttggacgc tcggagaaag ttgctggaaa ttacgaactt gcaagtggct ggtatcttcc
     6241 cggtactccg accgacagta ttttcagaag aaactacctg gaacttccgg gaattttttc
     6301 tttaggattc tctacctctt attcaaacaa tttttatgct tcatttaatg tggatattcc
     6361 tgtcggagac acttcttttt tcagatacgg atatgagaat atctttgaga tgaaggatat
     6421 gactcatata gggttagatt ttccaaaaaa ctcatatcta tcatacagca cagataccat
     6481 agatttttca ttcggaagga ataccatctc atacggacct ttaaaatata atctgctcct
     6541 ttccgatagt gctccatact acgataacct gagcatgaga gtaaaatccg gaaacctgaa
     6601 ctattctttt tctgctcttt catcaatccc gatgcttagc aaacaggagt acgaccagca
     6661 gttctctttc ggcaattttt atgcctaccg aaataccttt gttaacaggc ttgatctttc
     6721 cttcaaaaac tttgattttt cggtatccat gcttaatcat accggaggca ggcttgctga
     6781 tattagagac atgtttttgt tttcagataa cggacttttt tcagcagact caagatacag
     6841 aggttttttt gaaatatata ctgcatactg tttcaactac agtaatttta agaactcttt
     6901 tgccgcaggc atatcaaaaa ctatagatgc tggtgatgtg ctcataagag cttgtggaga
     6961 actttatcat gttcaggacg gtatatacga tcatgaagtt cctctgaaaa agctctacta
     7021 caggaccatt tcagtatcca acaatcccgg atccagaaac tttcctgata ctccctttgg
     7081 ttttttttat ggtgaaaatt ccaatatctt ctcagcagag gcggtaattg catgggaaga
     7141 cggatatatt gaagtagtga aagattttgg caataacagc tacggaaaag ttgaacattt
     7201 tttagccaga ataaatgcaa aaatgccttt cgcagatttg aatatacttc tttacgatac
     7261 taaaaatgca gataaagatt actttggttt ttccttcttt tatatacttg aactgggagt
     7321 cataatatga aataccctat tcttgttctt tctattgtgt taagtgtgac tttggtattt
     7381 tcagcaatag taactgataa aaccgatctg tacagatcgg tgttttatgg cggatatact
     7441 tcaaatattt atcctcgccc atcgggagaa gattttgcag ataaaagtac ttttggatac
     7501 gatatatata tttatgataa agacaagtac tatctgcttt ttaaagcata ccccttctgg
     7561 atgattgcag gaaagtatcc tacagattgg ggagatcttg agagaggagt atatctttca
     7621 gatagccttc cctatgtaaa aggtattaca tcgggatacg aaaacgaaaa acttagagtg
     7681 atctttggta tctatacttt cgatgcagcg ttatctccgc aggagcttat ccggcagaag
     7741 gaccaaacta tagattcaaa ctcagaccgc aggccactta cgattccgct cagcgggtat
     7801 ggagatccat tcaaaactct tattatacac agactagagg taagaccttt ttcaggaatt
     7861 atgattgcag taaatgaact taatcttatc ggcggtaaga tggtggatat agttgatgtg
     7921 aacccttttg gtatactcca caacactttt ggagaaggct tttcaaacac aatgctcgga
     7981 gttgattttt cagcagtcat tccttatggg atgagcgtat acggacagtt tgcaatggat
     8041 gacttccttg ttccgcagac agaatcaggt tcccaggcat ataaaccaac ggcatttgca
     8101 tatggtgccg gcttaaggaa ggtttttaaa gagcataatc tatacatttc accaaagata
     8161 gaattctata agatctatac atggatgtac aacagatggc agcctctttt aaagtttacc
     8221 ggaagattcg gaggaatcga tattcctatg gggtttgatt atggaaacga tatgcagggc
     8281 atgcttattg gagccgatat ctttaccgat gaaaaaatga aaatcttttt aggagctgaa
     8341 tactatcaaa aaggttcggt agatctgaac acttcatact ccgataacga aaagaagaaa
     8401 ctaacacaat tctggtgtga tttatttcca cctgtcacag atttttttgc actgagcctg
     8461 aaaattgata tttcgtattg aagggggata aaaatgaaaa tcgcactgat acttggaaca
     8521 agaccagaag caataaagat ggctccggta tactttgaac ttgaaaaaag aggggaacaa
     8581 gctgatatta tttcaacagg gcaacaccgt cagatgcttg accaggcttt acagatattt
     8641 gatattaaac ctaggtttga tcttcatgtt atgacagaca ggcagtccct ttcgctgctc
     8701 acacaaaaaa taattgccgg tctgcaggag ctttttgaca gagaaaagta cgatattgtt
     8761 cttgttcagg gcgatacgac tacttccttc tgcggagcac tggtgagctt ttataacaaa
     8821 attccggtag gtcacattga agccggtcta agaactaaca gcatctacga tccatttccg
     8881 gaggagataa acaggcgtct tacatccaag atagccagta tgaactttgc cgcaacttca
     8941 gtcagcgcag acaatctact taaagaagga attacggaga aaagtatatt tattacaggg
     9001 aacacagtta tcgattccat aaaatggatt caaaaaaaca aacagtttga taccaatgca
     9061 ataagacaga agtataatgt agcaaacggg aagtatattc ttatgacttt acacagaaga
     9121 gaaaattatg gcaggcctat ggaagaaatt ttcagagcag taaaaaaatt tctccaatac
     9181 aatcccgagt acaagctgat ctttcctgtt catatgagtc cggatgtcag ggagtatgta
     9241 tataagaata tggagggagt gaagaacgtt atacttactc agcccgtaga ttatattgat
     9301 tttatagctc ttatgtctga aagttctttc atacttaccg attctggggg tattcaggaa
     9361 gaggctccta ccttcggcat acccactgtt gtgctcagaa atacaactga aagaacggag
     9421 gcattatcag ccggaacagc tattttagca ggaacttacg aaaatgaaat tctggcatgt
     9481 cttgagctta tgactacaga aaagtgctta gagatggcga aaataaaaaa tccatatggc
     9541 gatgggttcg catcccaaaa aatagtagat atactacagt catactaaaa aattccaaat
     9601 cctcattgta acttaagttg aaatcgtttt ataaaatcaa aaaaacatca gcatttaaga
     9661 gtaatgtaga cgttaacaaa tttcttcata atgaaatatg tgagtaatat aacaaggata
     9721 tacttactat gaaaataatt tcatggtagg aggtgctgaa atgaaaaaaa catatgaaag
     9781 cgatacccag gtgataaggg ataacgatat ttattcagca gaggaactga cagaaaagat
     9841 taaaatagaa aaactggctg atgttctttt atacataaga cagttctaaa acgcttaaag
     9901 atggcgaaag ccatcttttt ttttgttttt ttgttcatct taattcactt acaagtagga
     9961 ttaagttaac taaaaagcaa ttgacaaagg aagggagaga tggtaagata tataaccgtg
    10021 ccgcgaaaaa agcacgaaac gaaaaaaagc agttcattga aaagtggata gaggaagaag
    10081 agaaagacca aaaggaagaa gaaaga
//
LOCUS       DAOSHR010000068         9928 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_5804_length_9928_cov_4.910159, whole genome shotgun sequence.
ACCESSION   DAOSHR010000068 DAOSHR010000000
VERSION     DAOSHR010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 9928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9928
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5804_length_9928_cov_4.910159"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2314
                     /locus_tag="PLU28_05160"
     CDS             <1..2314
                     /locus_tag="PLU28_05160"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOG34490.1"
                     /translation="SGKVLLERVALKSYVPLVSYESYVSRYESLKSENNFFLSIEAES
                     IDYKNDTSVNPFTSRDIQVVPYDTNRLMLNTMGEKSWSKSGQEVFYTFSVPSDGFYSF
                     GFKYLQAGKANSSVYRRMRIDGEILCTVCDAIKFDYTTSLNTLKMAYPLYLSQGEHTL
                     SLEVNGGVYSEYIDILYETIEEVNSLALEIKKLTGGNTASKNTEWDILNYFPKIQQQL
                     GGYIEKLEHIYNKVLQLNNQKGSSQELISLAGAIRSLSFLRKEPDLIPRRFSLLNEGT
                     SSVVKELSKALTEFENQPLQLDQIYIYTDEKALPDYRVSFLKRISEGFKRFFFSFLPK
                     NKSFSKDGTVLRVWVNRSRNYVELLQKITDSDFTAKTGIKVDFSIMPDEQKLILSNAS
                     GTSPDIALTISNWLPFELGIRGAALDLRQFSDFGEVICEFAPGALLPYVYEKGCFGLP
                     ETQDFYVLFYRKDIMEKLNLPIPATWDEVMLILPELQRYGMNFYLPIAGASGFKPVTV
                     TTPFIYQYHGELYSEDGFRTALDEPNSLKAIELMTKLFTLYGLELQVPNFFEHFRSGL
                     SPIGISNFTTYVQLNVGAPELKNSWDIALAPGIKNENGVVERWHAGSAQACMIFKNTK
                     NPESSWEFIKWWMSQETQSSFGYQVQALYGKEYMYNSANLRAFGQIPIPSEHKTIALQ
                     QWEWMKEIPKTPAGYMLERELSNIWIKIVLQGKNTRAAVDESVIRINKEISRKLEEFG
                     YSVDGVKIKDYYIPTIEDIAGWRNDYEEKQ"
     gene            2298..3188
                     /locus_tag="PLU28_05165"
     CDS             2298..3188
                     /locus_tag="PLU28_05165"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOG34491.1"
                     /translation="MKKNNKSLGAYIFLSPYLLLFTVFIVLPVIMAIVLSFTNFNAVQ
                     FPQFTGLKNYVSMITKDDVFMQYVITNTLKFSIIVGPVGYILSFLLAWMLAQIPLIPR
                     TILALVIYSPSMTMGAAMQVIWKALFSGDINGYINSLLIRLDIIQQPIQWLQSPQYLM
                     PIMIVVTIWSSMGVGFLAMLAGILNVNPELYEAGYIDGISKKWQEIVYVTIPSMKPQM
                     LFGAVMSVVSTFQAGAIGVQLSGSNPTPQYAGQLIVNHIEDYGFLRYEMGYAAAVSVF
                     LLLLIWLSSKVAWKFFGERD"
     gene            3205..4077
                     /locus_tag="PLU28_05170"
     CDS             3205..4077
                     /locus_tag="PLU28_05170"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOG34492.1"
                     /translation="MSKFQGTKINPKNFHKSQIKFYLFLVPFAVFMVLPLVFIFSQAF
                     KPMDELLQFPPKILVAHPSFENFRQLFSTMTNSGLPVTRYLFNSITVTAITVILTVYI
                     SVSAGYALSKKKFGLRNFIFKVNTFALMFVPIAVSIPRYIIVVKMGLIDTFVINILPM
                     LAMPVGLFLVKQFIDQIPDTLIEAAKIDGANDFIIFFRIIIPLCKPAIATLVILSFQT
                     AWNYTEASSIYINNESFKTFAYFMNTVTSSNNTVAGMGMAAAAALIMFLPNLIIFIFT
                     QSKVMNTMAHSGIK"
     gene            4088..6079
                     /locus_tag="PLU28_05175"
     CDS             4088..6079
                     /locus_tag="PLU28_05175"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HOG34493.1"
                     /translation="MKIKPLTLLVILLFFAVSYADVPYKTYTMGPNGFIVETRNAFVP
                     EKFFFPSIMNAEDIFVDKDGSIYVADTGNMRVLKIKSDMSEEVFSDYMFFMPTGIFVT
                     DELVYVADSGNFSVCVFNKDGELLKELGRPSQPLFGSKNQFIPLKVAVDARNNIYLIS
                     EGSTDGIVCLNSDGEFMNYLGANRPDVSLKMLLQRIIFTGAKGQFLKLRPPSPTNIAI
                     DADGIIYTCTYGLETNSIKKFNIAGVNIFENRIFGDPDIVDIGLDDLNNVYAVDSNGF
                     IVVSNSLGDLLFIFGNKDSLAERKGLLTNPSALEVAGDGRVYVLDKEKNSITVFKPTD
                     FADLVFRADNMYIQGLYLQGKDEWQKVQTFNSSFLLSYKALAKAYFKEGMYGQSLEYF
                     RLSQDKGEYSESFWQIRNEWLQKNLSVIFIGFVAVFILYKLLRFIDRKKNIFSSLKLF
                     FVKLSRVKLINDLLFLKYMFFHPIDGYYEIRRKNRISLVSATVLYIYLFFLELFKIFF
                     TGFIFSSVSVYRVNLFEVAAGIYLPVAVLLLSNYLISEINSGEGRLRDIYTAGAYALA
                     PYIVFEPFLIIISNVLTYNEAFLYTFPQYVVIGWTLILLFLMVKQIHNYTVGEAVKNI
                     LLTLFMVFIIIIIVFIIFVLFTQEAEFLQSIFREVILRE"
     gene            6072..8132
                     /locus_tag="PLU28_05180"
     CDS             6072..8132
                     /locus_tag="PLU28_05180"
                     /inference="COORDINATES: protein motif:HMM:NF039504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5696 domain-containing protein"
                     /protein_id="HOG34494.1"
                     /translation="MNRRIIFVIIVFLCACIALSNFEKVYENDNYTYFFDSHSLLLRI
                     EQKNGDNVWHTGIEQDDGFLNDEWQAFSSSGMTVEYMTGDMQSKKVSASSDTCSITVQ
                     VKGNMLQINAQFREQGFSVGMEILFNEQGFEVTSGKNFISESSDTGNRIMNLYFFPFF
                     EAAIKSDNGYVFIPDGSGSIMKIGKSNAKKPYIQRVYGYDYGTADTASLSTGYGINPA
                     KKIKIPVYGVVSQEDKKGFMSIVKQGDEYTEILAYPGGVITPYTWISARYIVRQMYKK
                     FLDKKGTAVLVSTQEPYDLTVKTGFILLCSQDANYSSMAVKYREYLEENNMLSEKFSS
                     EKALLRLDVMAAETRNRVIGKDYIQMTSLKELKEITGMLNGLDYIINIFGYSKDGYSQ
                     SFPVHLPVSQSFAGLKELKEFNLSNKNVFYMVDYTLANKEKTGFGADVAQNISKQFIT
                     SWGYRILSPEVSANLYKNEQKKFEELNITNLSFRTTGEVLYSDYGKRPLSRGQAKQII
                     SSSFNGAFYNPNVYLWKSCSAIFDIDTDSSGYIFEKRSVPFLQILLSGKIPCFSKPLN
                     FTSDLGNELLKLVEFGVYPSFIITQESSLKLLETPSQWISSSEFSSWQDTVRSSYDFV
                     NEALKEVYGCGIISHEIFENGISKTRYSNDVVIFVNYGTQSMEYGSIEVPARGYRVVK
                     DGEI"
     gene            8129..8983
                     /locus_tag="PLU28_05185"
     CDS             8129..8983
                     /locus_tag="PLU28_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOG34495.1"
                     /translation="MKLTARNSAKGYFFISGWIIGFLIFTLIPIIRTFWFSFNRVRFT
                     ADGIKESFIGLLNYKNAFLMDVAFTDGLINYAGQMAVFIPVIITFSLIAAILLTKDIK
                     GKGFFRTIFFLPVIITSGPVMKQLIEQNATSLPGISEYFSVQELANEFGLLGNLLGTV
                     LSSFIMILWFCGVQILIFIAAIQKLDQSMYEAASIDGASGWQSFWKLTLPALMPVVGV
                     NVLYTLVTLSGFSLNTIVQKISTDMYDINRGIGYATALSVIYFGVILIILTVFMVFIN
                     GRRKKAER"
     gene            8992..9882
                     /locus_tag="PLU28_05190"
     CDS             8992..9882
                     /locus_tag="PLU28_05190"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOG34496.1"
                     /translation="MKKKNIKTVFSKAVIYFLLVLIGFVFLYPILYMVSYSFMDTQDL
                     VNPFVKWIPTKLYTDNYVRACQVLDYFPTLIKTIYVNLIPAVLQTIFTSFTGYGLARF
                     EFRGKKLVMLLILVTFIIPAQVTMIPQFLMYKDLNLIGSLYSYILPAIFSQGLKSSIF
                     ILIFYQFFKMLPKSLEEAAQLDGASFMVIFTKIAVPSALPAYIVSFIFSFVWYWNETT
                     LSALYFGNKIKTLTLGLQNFAATYQNVYSMSVTQGGKNLNEAISMAGTLISILPLLIF
                     YFIFQRWFVQSVDKTGITGE"
BASE COUNT         2873 a         1792 c         2136 g         3127 t
ORIGIN      
        1 cagcggtaaa gttcttttgg agagggtggc tttaaaatcc tacgttcctc ttgtttcata
       61 tgaatcttat gtttcaagat atgagtcttt aaaaagtgaa aacaattttt tcctttcaat
      121 agaagcagaa agtatagatt ataaaaacga tacttctgta aatcctttta ccagccgtga
      181 tattcaggtg gttccgtatg atacaaacag acttatgctc aacactatgg gtgaaaagtc
      241 atggtccaag agcggccaag aggtttttta taccttcagc gtgccgtctg acggatttta
      301 tagctttggt tttaagtacc ttcaggccgg aaaagccaat tcaagtgttt acagacgtat
      361 gagaatagac ggagagattt tatgcaccgt ctgtgatgcc ataaagtttg attatacaac
      421 ctcgttgaac actcttaaaa tggcttatcc gctttatctt tcgcagggtg agcacactct
      481 ttcgcttgaa gttaacggag gagtgtattc ggagtatata gatattcttt atgaaacaat
      541 agaagaggta aacagtctgg ctcttgaaat aaaaaagctt acaggaggaa ataccgcttc
      601 caaaaacaca gaatgggata ttttgaatta ttttccgaaa attcaacaac agctcggcgg
      661 atatatagaa aagcttgaac atatttataa taaagtgtta cagcttaaca accaaaaagg
      721 tagcagtcaa gagcttatct cgcttgcagg cgcaataagg agtctgtctt ttctgagaaa
      781 ggaaccagat ctgattccaa ggaggttttc acttctgaat gaaggaacta gctctgttgt
      841 aaaggaactt tctaaggctc ttaccgaatt tgagaaccag cctttgcagc ttgatcagat
      901 ctatatatac acagatgaaa aagctcttcc ggattatagg gtttcttttt taaaaaggat
      961 aagtgagggt ttcaaaagat ttttcttttc ttttctgcct aagaataaaa gcttttcaaa
     1021 ggacggtact gttctgaggg tgtgggtaaa ccgttcaagg aactatgttg aactgcttca
     1081 gaagataact gacagtgatt ttacagctaa aacaggtata aaggttgatt tttcgattat
     1141 gcctgacgaa cagaagctta ttctttcaaa tgcatccgga acttctccgg atatagccct
     1201 gactataagc aactggcttc cttttgagct tgggataaga ggagcagccc ttgatctgag
     1261 acagttctct gactttggtg aggttatatg tgagtttgct cccggggcgc tgctgccgta
     1321 tgtatatgaa aagggatgct tcggacttcc tgagacacag gatttttatg ttttgtttta
     1381 ccgcaaggat ataatggaaa agcttaatct tcccattccc gcaacttggg atgaggttat
     1441 gcttatttta ccggagcttc agagatatgg aatgaacttc tatcttccca tagcgggagc
     1501 ttccgggttt aagccagtta ctgttacaac gccctttatt tatcagtatc atggtgaact
     1561 ttattctgag gacggcttta gaacggctct ggatgaaccg aactctttaa aggctataga
     1621 gcttatgacc aaattgttta ctctatacgg gctggaactt caggttccaa atttctttga
     1681 gcattttagg agtggccttt cccctatagg cataagcaac tttactacat atgttcagtt
     1741 aaatgttgga gctcccgaac ttaaaaattc atgggatata gctcttgctc cgggtataaa
     1801 aaacgaaaac ggtgtggttg aaagatggca tgctgggtca gcacaggcat gcatgatctt
     1861 taaaaacaca aaaaatccgg aatcttcatg ggagtttata aaatggtgga tgtcccagga
     1921 gactcagagc agtttcggtt accaggttca ggctctgtac ggcaaggagt atatgtataa
     1981 cagtgccaac ctccgggcgt tcggacagat accaatacct tcggagcata aaaccatcgc
     2041 tttgcagcag tgggagtgga tgaaggaaat acctaaaaca cctgcagggt atatgcttga
     2101 aagagagtta agtaatatct ggatcaagat agttctccag gggaaaaata cacgcgctgc
     2161 cgtagatgaa tcggtaataa ggataaacaa ggaaatttcc agaaagcttg aggagttcgg
     2221 ctacagcgta gacggagtaa aaattaagga ttattatatt ccgacaatag aagatatagc
     2281 ggggtggagg aatgattatg aagaaaaaca ataaaagcct cggagcttac atctttcttt
     2341 cgccgtatct gctgttattc actgttttca tagtcctgcc ggttattatg gcgatagtcc
     2401 tttcttttac caactttaac gcagtgcagt ttccgcagtt cacagggctg aaaaactatg
     2461 tgagcatgat taccaaagat gatgtattta tgcagtatgt cataaccaat acccttaagt
     2521 tttcaattat agtcggtcct gtcgggtata ttctttcctt cctgcttgca tggatgcttg
     2581 cacagatacc gcttattccc agaaccattc tggcactggt gatatattcg ccttcaatga
     2641 ccatgggagc tgctatgcag gtgatatgga aggctctttt cagcggcgat ataaacggat
     2701 acatcaacag ccttcttata cgtcttgata taatacagca gcctatacag tggcttcagt
     2761 caccacagta cctcatgccc ataatgatag ttgttacaat atggagcagt atgggagtgg
     2821 gatttctggc tatgctggca ggaatactca acgttaatcc ggaactttat gaggcgggat
     2881 atatagacgg aatatccaaa aaatggcagg agatcgtgta tgtgactatt ccgtcaatga
     2941 agcctcagat gcttttcgga gcagtaatgt cagtagtttc cacatttcag gccggagcca
     3001 tcggggtaca gctttcaggt tcaaatccta ctccgcagta tgccggacag cttatagtga
     3061 accatattga ggactatggt tttttgaggt acgaaatggg ttatgccgca gctgtttcag
     3121 tctttcttct tttgcttata tggctcagct ccaaggtagc ctggaagttt ttcggagaaa
     3181 gggattaaat tcaggtggtg ggaaatgtct aaatttcagg ggaccaagat aaacccaaag
     3241 aactttcata aaagtcagat aaaattttat ctttttttag taccttttgc agtttttatg
     3301 gtgctgccgc tggtattcat attttcgcag gcctttaagc ctatggatga gctgcttcag
     3361 tttcctccga agatcttagt ggcacatccc agttttgaaa atttcaggca gcttttttca
     3421 accatgacca actccggact gccggttaca aggtatctct tcaacagcat taccgtaacc
     3481 gcaattaccg ttatacttac ggtttatata agcgtaagcg ccggatatgc actttcaaaa
     3541 aagaagttcg gcctgaggaa ctttattttc aaggtaaata cttttgcatt gatgtttgtt
     3601 cccatagccg tttctattcc caggtacata attgtggtaa agatgggact tatagataca
     3661 tttgttataa atatccttcc catgctggcc atgcctgtag gtctttttct ggtcaagcag
     3721 tttatagatc agataccgga tacgttaata gaagcggcta agatagacgg agcgaacgat
     3781 tttataatat tcttcaggat tatcataccg ctctgcaaac ctgctattgc gacacttgta
     3841 attctttcat ttcagacggc ttggaactac acagaggctt catcgatcta tataaacaat
     3901 gaaagtttta aaacctttgc ttattttatg aataccgtta cttcatcaaa caatactgtg
     3961 gcgggtatgg ggatggcagc cgctgcagct cttataatgt ttcttccaaa tcttatcatc
     4021 tttatcttta cacagagcaa agtcatgaat actatggctc attccggaat caagtgaggt
     4081 ggggagattg aagataaaac ctttgactct gcttgtaatt ctgctgtttt ttgcagtatc
     4141 gtatgcggat gtgccgtata aaacctacac gatggggcct aacggattta tagttgaaac
     4201 acgcaatgcc tttgttccgg aaaagttttt ctttccgtca ataatgaatg cggaggatat
     4261 ttttgtagac aaagacggta gtatatatgt tgctgacaca ggaaacatga gagttttaaa
     4321 gataaaatcc gatatgagcg aggaagtttt ttctgactat atgtttttta tgcccacagg
     4381 aatttttgtt acggatgagc ttgtatatgt tgccgattcc ggaaattttt ccgtttgcgt
     4441 tttcaataag gacggagagc ttttaaaaga gctaggcaga ccatcacaac cgctttttgg
     4501 aagcaaaaac cagttcatcc cgcttaaagt tgccgtagat gcaagaaata atatatatct
     4561 cataagtgaa ggatccacag acggcattgt ctgtcttaac agcgacggag agtttatgaa
     4621 ctatctggga gccaaccgtc cggatgtttc gctaaagatg cttcttcagc gtataatatt
     4681 taccggagcc aaaggtcagt ttctcaagct cagaccgcct tctccgacta atattgcaat
     4741 agatgctgat ggcatcatat atacctgcac gtatggatta gaaaccaaca gcattaaaaa
     4801 attcaatatt gcaggtgtta atatttttga aaacaggatc ttcggtgatc cggacatagt
     4861 agatataggg cttgatgatt taaataatgt ttatgcggtt gattccaacg gttttatagt
     4921 tgtatcaaac agtcttggcg atcttctttt catctttgga aacaaggatt ctcttgccga
     4981 aaggaaagga cttttgacta atccttcagc gctggaggtt gcaggagatg gcagagttta
     5041 tgttctggac aaggaaaaaa attctataac ggtttttaag cctacagact ttgcagatct
     5101 tgttttcaga gctgacaata tgtatatcca gggtctttat ctccagggga aggatgaatg
     5161 gcagaaagta cagacgttta acagttcttt tctgctttcc tataaggctc ttgccaaagc
     5221 gtattttaaa gagggaatgt acggacagtc tcttgagtat ttcaggttat cgcaggacaa
     5281 gggagagtac tcggaatctt tttggcagat acgcaatgaa tggcttcaga aaaacttatc
     5341 tgtgatattc ataggctttg tggcagtttt cattttatat aaactgctta ggtttataga
     5401 ccgtaagaaa aatatttttt catcacttaa actatttttt gtaaagcttt caagggtaaa
     5461 gcttataaac gatctgttgt ttcttaagta tatgtttttc catccgatag acggatacta
     5521 tgaaataaga cgtaaaaaca gaatctcact agtgagtgca acagtccttt atatctatct
     5581 ttttttcttg gagcttttca agatcttttt tacaggtttt attttttcat ctgtttcggt
     5641 atacagagta aatctctttg aagtagctgc agggatttat cttccagttg cggttctttt
     5701 gctttccaac taccttataa gtgaaataaa cagtggtgaa ggaaggctga gggacatcta
     5761 tacggcagga gcttatgcac tggcacctta tattgttttt gaaccttttc tgataatcat
     5821 aagcaatgtg ctgacttata acgaagcatt tctctatact tttccgcagt atgtggtgat
     5881 aggctggact ctgattctct tgttccttat ggtcaagcag attcataact atacggtagg
     5941 cgaagcggtt aaaaatattc tcttgacact tttcatggtt tttataataa tcatcattgt
     6001 gtttataatc tttgtgcttt tcacacagga ggctgagttc ttgcagagca tattcagaga
     6061 ggtgatattg cgtgaataga agaataattt tcgtgatcat agttttttta tgtgcctgta
     6121 tagctctttc caattttgaa aaagtatatg aaaacgataa ttatacatat ttttttgatt
     6181 ctcatagcct ccttttacgc atagaacaaa agaacgggga taacgtatgg cataccggca
     6241 ttgaacagga cgacggattt cttaatgatg aatggcaggc cttttcttca agcggtatga
     6301 ctgttgaata tatgaccgga gatatgcaga gcaaaaaggt gagtgcctca tcagatacat
     6361 gcagtataac cgtgcaggtc aaaggtaata tgctgcagat aaatgcacag ttcagagagc
     6421 agggcttttc agtgggtatg gagatactct ttaatgagca gggctttgaa gtaacttccg
     6481 gaaagaattt tataagcgaa tcttctgata ccggcaacag gataatgaat ctttattttt
     6541 tccctttttt tgaagcagca atcaaaagcg acaatggata tgttttcatt ccggacggaa
     6601 gcggcagtat aatgaaaatc ggtaagtcca acgctaagaa gccttatatt caaagagtat
     6661 acggatatga ttacggcacg gctgatactg catctctttc gacgggatat ggaataaatc
     6721 ctgccaaaaa gataaagata ccggtttatg gagtcgtatc gcaggaagat aaaaaagggt
     6781 tcatgtccat agtaaagcag ggggatgagt atactgaaat acttgcctat cctggaggag
     6841 tgataacgcc gtatacatgg ataagtgcaa ggtatatagt aaggcagatg tataagaaat
     6901 ttttggataa gaagggcaca gctgttcttg tcagcacaca ggagccatat gatctgacgg
     6961 tgaaaaccgg gtttattctt ctttgctcac aggatgctaa ctacagttct atggctgtaa
     7021 agtatagaga gtatcttgaa gaaaataata tgctttctga gaagttttct tctgagaaag
     7081 ctttactaag gctcgatgtt atggcggctg aaacaaggaa cagggtaatc ggaaaagatt
     7141 atatacagat gacttcgctg aaagaactta aagaaataac gggaatgctt aacggacttg
     7201 attatataat aaatattttc ggatattcca aagacggata cagtcagtcc tttcctgttc
     7261 atctgcctgt gagtcagtca tttgcgggtt taaaggagct taaggagttc aatctttcca
     7321 acaaaaatgt tttctatatg gtagactata cattggccaa taaagaaaaa acagggttcg
     7381 gagcagatgt ggctcagaat atatcaaagc agtttataac aagctgggga taccgtattc
     7441 tatctcctga ggtttcggcc aatctttata aaaatgagca aaagaagttt gaggagctta
     7501 acataacaaa tctttctttc agaacaacag gagaggttct ttacagcgat tacggtaaaa
     7561 gacctttatc cagaggacaa gccaaacaga ttataagcag cagcttcaac ggagcttttt
     7621 ataatccaaa tgtttatctg tggaaaagct gcagcgccat ttttgatatt gataccgact
     7681 cttcgggata tatttttgag aagcgttcag taccgttttt acagattctt ctttctggta
     7741 aaattccgtg tttttcaaaa ccgctgaact ttacatccga tcttggcaat gagcttttaa
     7801 agcttgtcga atttggagta tatccctcgt ttataataac gcaggagagt tctttaaaac
     7861 ttttggaaac gccgtcacag tggataagtt cttcggaatt ttcctcatgg caggatactg
     7921 tacgcagttc ttacgacttc gtaaatgagg cgcttaaaga agtttacggc tgtggtatta
     7981 tatctcatga gatttttgaa aacggtatat ccaagaccag atactcaaac gatgtcgtta
     8041 tatttgtcaa ttacggcaca caaagcatgg agtacggcag tatagaagtt ccggccagag
     8101 gttatcgtgt agttaaggat ggtgagatat gaagctgact gctaggaaca gcgcaaaagg
     8161 ctattttttt atttcaggat ggatcatagg ctttcttata tttacgctta ttcccataat
     8221 aagaaccttc tggttcagtt ttaacagagt gaggtttact gccgatggta taaaagagtc
     8281 tttcataggg cttttaaact ataagaatgc tttccttatg gatgtcgcct ttaccgatgg
     8341 ccttataaac tatgcagggc agatggctgt gtttataccg gttataatca ctttttccct
     8401 tattgccgcc attttgctta caaaagatat aaagggaaag ggatttttca gaaccatatt
     8461 ttttcttccc gttataataa ccagcggtcc tgtgatgaaa caacttatag agcaaaacgc
     8521 tacgtcactt cctggaattt ccgagtactt ttcggttcag gagcttgcaa atgagtttgg
     8581 gttacttgga aatcttcttg gaacggttct ttcgtccttt ataatgattt tatggttctg
     8641 cggtgttcag attctcatat tcatagctgc tatacaaaag cttgaccaga gtatgtacga
     8701 agcggcttca atagacggag catcaggatg gcagagtttc tggaagctta cactcccagc
     8761 tctgatgcct gtggtggggg tgaatgttct gtatactctt gtgacgcttt caggtttttc
     8821 acttaatacg attgttcaga aaatttcgac tgacatgtac gatataaacc gaggcatagg
     8881 ctatgcaaca gcgctttctg ttatttactt cggagttata ctcatcattc tgaccgtctt
     8941 tatggttttt ataaacggcc ggcgcaaaaa agcggaaaga taggcgatat catgaaaaaa
     9001 aagaatataa aaactgtttt ttccaaggct gtcatatact ttttgcttgt gctgataggg
     9061 tttgtttttc tctatccgat actttatatg gtatcttaca gcttcatgga tacccaggat
     9121 ctggtgaatc cttttgtaaa atggattccg acaaagcttt atactgataa ttatgtcaga
     9181 gcctgtcagg tgcttgatta ttttcctact ctgataaaaa ccatctatgt caatctaatc
     9241 cctgctgttc tacagactat attcacatcc tttacagggt atggcttggc acgttttgag
     9301 ttcagaggaa aaaagcttgt aatgctttta atacttgtta cgtttataat tcctgctcag
     9361 gtaaccatga tcccacagtt tcttatgtat aaggatctca atcttattgg aagtctttac
     9421 tcatatatac tccctgccat attcagccag ggtttgaaaa gttccatctt catactgatc
     9481 ttttaccagt tttttaagat gcttccaaaa tctcttgagg aggctgccca gcttgacgga
     9541 gcaagcttta tggttatttt cacaaaaatt gccgttcctt cggctcttcc tgcatacatt
     9601 gtgtcgttca ttttttcgtt tgtgtggtac tggaatgaga ctacgctttc ggcgctgtat
     9661 ttcggaaaca agattaagac tctgacgctt ggtctgcaaa attttgctgc tacttatcaa
     9721 aatgtttact caatgagtgt tacgcagggc gggaaaaacc ttaatgaagc catttcaatg
     9781 gccggaacat tgatctcgat acttccgctt ttaatatttt atttcatatt tcagcgttgg
     9841 tttgttcaga gtgtcgataa aaccggtata accggagaat agagggggag tttatgaaaa
     9901 aagcattttg gtgtttactg gtactggc
//
LOCUS       DAOSHR010000069         9913 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_5813_length_9913_cov_6.100223, whole genome shotgun sequence.
ACCESSION   DAOSHR010000069 DAOSHR010000000
VERSION     DAOSHR010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 9913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9913
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5813_length_9913_cov_6.100223"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1544)
                     /locus_tag="PLU28_05195"
     CDS             complement(<1..1544)
                     /locus_tag="PLU28_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HOG34497.1"
                     /translation="MFDNFTERAAKVFIEAQNEARDMGHPYVGTEHLLLGLLKVGGRY
                     LDLVFKQFDLNYNKIKNEITQVVGTNASQGIIGSPQPTPRSKRIIELAYDESELMGSS
                     RIDAEHILLGICREAEGIASHILKRTGVNLTDMRKKLAEAMMKEEVVPESASESNIED
                     EERKKQKQSVLKQLEDFGTDLTAKCRDKLLDPVIGRQTEITRVMEVLARRKKNNPVLI
                     GEAGVGKSAIVEGLAQKIVDGDVPEVLKNKTIFSLDITSVVAGTKYRGEFEKRMKKLM
                     QVLEKADDVILFVDELHMIVDAGAAEGSSMDAANVLKPALANGDITMIGSTTSSEYRK
                     FIEKDPALERRFQKIYVSEPTTEECVKILQGIAKKYEEHHKVSYSLSSLDSAVNLSVR
                     YITDRFLPDKAIDLIDEAGAKARLKALTLPENLKAVVKSIEAMEADKNIMISKNDVAQ
                     AEKLTAKLDKVKEEYKQKYLEWRKKAENVVIDISSEDIANVVSSWTGVPLKKLESTEI
                     EKLLNL"
     gene            1677..2246
                     /locus_tag="PLU28_05200"
     CDS             1677..2246
                     /locus_tag="PLU28_05200"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOG34498.1"
                     /translation="MELTKYSHKEEKFTDELVKMIYFTDKDISRYLFSDDYAKAAYAI
                     KMMLIKNEYIFLNDCINILYDKSDLLGFSLAYSGEDKKKFILDTEMEKKIKALEEILT
                     SNISDRQMYLHYIYTDKAVRGKGAGSMLMQKVFSEAKDKECTELLLDVAADNSNAISF
                     YKHKGFSVYEEKKNVSINIIGSLCMKMKI"
     gene            2350..3222
                     /locus_tag="PLU28_05205"
     CDS             2350..3222
                     /locus_tag="PLU28_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34499.1"
                     /translation="MRKYIMFIIIVITLLIPFSAAARDNKGIITDPAKELKWNYFVDN
                     LLYARFLNLQEYSALRGLGLNDYVLIDKPGEINDKLIGSLSIGNRMFPILNSLTGITD
                     FYDVYFMNASYIKDEPVLTFLVKSFFEDDLPPGYAWSLIARYVKALFHLSFVDVEIKV
                     NETYTLEGKNNIGELVTRLYAKNSSLYPFGNWDRSYAPVRINNNPYYTMIEHLGSIQK
                     SYNSNQKTLYLRNLFPIAGWSTNWNSNFSNPEDIIYLFPELELYADFSIPFNQSPGDY
                     VLVLDMQISPSPYF"
     gene            3310..3385
                     /locus_tag="PLU28_05210"
     tRNA            3310..3385
                     /locus_tag="PLU28_05210"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3343..3345,aa:Thr,seq:tgt)
     gene            3401..3487
                     /locus_tag="PLU28_05215"
     tRNA            3401..3487
                     /locus_tag="PLU28_05215"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3435..3437,aa:Tyr,seq:gta)
     gene            3525..3689
                     /gene="rpmG"
                     /locus_tag="PLU28_05220"
     CDS             3525..3689
                     /gene="rpmG"
                     /locus_tag="PLU28_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HOG34500.1"
                     /translation="MATKNQVVQFSLKCSECGTRNYYKQKSRLFKNKIELSKYCPKCN
                     KHTKHNESKI"
     gene            3747..3947
                     /gene="secE"
                     /locus_tag="PLU28_05225"
     CDS             3747..3947
                     /gene="secE"
                     /locus_tag="PLU28_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HOG34501.1"
                     /translation="MSKFWVFLSQVWQEAKKVSWPSKKELLNSTVIVLAILLFFAVYL
                     FAVDLGLMTFFKNLIYPITFGG"
     gene            3975..5036
                     /locus_tag="PLU28_05230"
     CDS             3975..5036
                     /locus_tag="PLU28_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HOG34502.1"
                     /translation="MRKEWYIAQAYSGMENKVKEVIEEKAKMLGLSSLFGRVIVPEIE
                     EIDYANRKVDRVFISNEAELYTKASKYVKKGDILAKEPSIYCKKTGAVTEVKNYRRII
                     VETVDKKYSKTFVIPESAGIIAGLRTGRDIKSGMPFAKSAAYECDVDGEIGSIEKVKR
                     VVIQSSLNDEEVYVIPVKLFDSKTLRIGAKIDSGKKLANGSEYAAKSSGRVDIKEHLM
                     RKEIKIIKTNKSKLFPGYVFIEMIYTQESENLIKTVPYISTLLNVGGKPIKLTLKEAK
                     ALLKLVGQELVEQKKLPKVRMDYTIGEQIKIINGPFELFTGKITKINLEKQEVKVTIT
                     LFGRETTVELSLAEIEKIE"
     gene            5108..5533
                     /gene="rplK"
                     /locus_tag="PLU28_05235"
     CDS             5108..5533
                     /gene="rplK"
                     /locus_tag="PLU28_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOG34503.1"
                     /translation="MAKKLVRVVKLQLEAGKATPAQPVGPALGQHGVNLMEFCKKFNA
                     ATADKAGNVIPVEISIFEDRSFTFVLKTPPASFLIKKAAGVKTAAKTPSKEIVGKITS
                     KQVRDIAELKMKDLNAMTIEAAMRIIEGTARNMGIEVVE"
     gene            5550..6245
                     /gene="rplA"
                     /locus_tag="PLU28_05240"
     CDS             5550..6245
                     /gene="rplA"
                     /locus_tag="PLU28_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOG34504.1"
                     /translation="MAKHGKKYNEVKKLVDRTKFYTLDEAIELVKKMCYAKFDESVEV
                     HVQLGIDATKSDQNVRSTVSLPHGIGKTVRIAVFATGDKAKEAKDMGADFVGAGDLVE
                     KVMKEGWTDFDVAIAAPDMMKDVGKLGKVLGPKGLMPSPKAGTVTADIGNAVKEFKAG
                     RIEVRNDKTGNVHFPVGKKSFDDIKIKENIVAGLEQLNKLKPTTSKGKFIKKVTLAST
                     MSPGVKLDNSVSL"
     misc_feature    6269..6405
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            6414..6965
                     /gene="rplJ"
                     /locus_tag="PLU28_05245"
     CDS             6414..6965
                     /gene="rplJ"
                     /locus_tag="PLU28_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOG34505.1"
                     /translation="MLTKEKKSALIDEISSALKTSPIVVFTDFSGMPVAASNKMRMEM
                     YKQFGDNATYRVGRNTLIRTAVKKAELSVQEYSALIDGSTGVFYINDSVDPIEALKFL
                     TEFAKKNSNRPVVKGGMLEGVIFDAKQAAEYAKLPSKQQLIAMVLGSLNAPISGLVNV
                     MAGTLKKVLYALNAVKEQKEKAE"
     gene            6995..7375
                     /gene="rplL"
                     /locus_tag="PLU28_05250"
     CDS             6995..7375
                     /gene="rplL"
                     /locus_tag="PLU28_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOG34506.1"
                     /translation="MTKEELIQAIKSMTVADLAELVKALEEEFGVSASAPVAVAAAPA
                     AAAAAPVEEKTDFTVVIKSFGANKVGVIKVVREITGLGLKEAKDLVEKAGTPDAKVKE
                     NAPKAEAEDIKKKLVEAGAEVELK"
     gene            7521..>9913
                     /locus_tag="PLU28_05255"
     CDS             7521..>9913
                     /locus_tag="PLU28_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOG34507.1"
                     /translation="MVNTRSVSVGRRSREFFGKVHEDHSMYDDLIKIQKDSFKNFVER
                     GMIESVRKYMPISVPIKTTGKKTKDIIVDFIDIKFEEPGVDEQECKYKDLTFSGKAYL
                     KIQITDSSTGEVLERDDVFLCNVPFMNDRGIFIINGAERVVVNQLVRSPGIYFVKEEE
                     KDSSKELFIAHFLPVKGAWFEIIYNPNEGKEVLQVRIDRKRKFNFFLFLRAIGYETDL
                     SILDLFPKEIDLDDEIEREKHLDSVVLTDLSYPELQDREPALTTIRGLRLKEALDLLQ
                     KYEVKKVTLANNIAQITLDKMKRRYEKDGALTSLEAYKEMYIKLKPTEIPKPQKAKEE
                     IDDMYFSHPNEPEKDKFDFSEIGRKKAQVRLKHVYKRYLEEVEGRTFSLEDGDKVEYT
                     INSKTIDKLDIILAARHLLEIESNQDYLDTRDHLGNKRVRAVGELMQIEFEKAFSKMI
                     QHIPEKVISMAQSLSKINPQSLINSRAIMTAFHQFFATSQLSQFMDQINPISELTHKR
                     RLSAIGPDGLKREHAKFEVRDVHHSHYGRMCPIETPEGANIGLITSLSILAQTDEFGF
                     LKTPYFRVKDAIVQYAGDKASSIVPLSADEEENYKIAPASIEIDEKGNIIPPYVEVRY
                     QGKVTFVPKKEVDYIAVTPKQIASVSAALIPFLEHDDANRALMGSNMQRQAVPLVYTE
                     APWVGTGVEWLAARDSGYLIFAKHKGVIEYVDGRKIVIARTLDEGEDCRTKEKDGKRY
                     LIDNEGNIIKDEYQILKYIRSNQDMCINQRPVVSKGQTVEKGDILADGPATDMGELAL
                     "
BASE COUNT         3341 a         1832 c         2047 g         2693 t
ORIGIN      
        1 tcaagattaa gaagtttttc aatctctgtg gattcaagct ttttaagcgg aactccagtc
       61 cagctcgaaa caacattggc tatatcctcg gaagatatat caattacaac attttctgct
      121 ttttttctcc attcgagata cttctgttta tactcttctt taactttgtc tagttttgca
      181 gttaacttct ctgcctgggc aacatcattt ttgcttatca taatgttttt atcggcttcc
      241 atagcctcta tactcttaac tactgcttta aggttttcag gaagggtaag agctttgagc
      301 ctggcttttg ctccagcctc atctataaga tctatcgctt tgtctggtaa aaatctgtca
      361 gttatatacc ttacagaaag atttacagcc gaatccagtg aagaaagaga gtacgatacc
      421 ttatgatgct cctcatactt tttagctata ccctgaagaa tctttacaca ttcttctgta
      481 gttggctccg atacatatat cttttgaaaa cgccgttcca gtgcagggtc tttttcaata
      541 aacttacggt attcactgga tgtcgtcgag cctatcatgg ttatatcgcc gttagccaaa
      601 gccggcttga gaacgttggc agcatccatg gacgaccctt cggccgctcc cgcatccaca
      661 atcatatgaa gttcgtcgac aaataaaatg acatcatcag ctttttcaag aacctgcata
      721 agttttttca tacgtttttc aaactctccg cggtactttg ttccggcaac cacagaggtt
      781 atatccagtg aaaatatagt tttgtttttt aaaacctccg gtacatcacc atcaactatt
      841 ttctgagcaa gaccttcaac tatagcgctt ttaccaaccc cggcctcgcc tataagaaca
      901 ggattatttt tctttcttct agccaaaact tccataactc ttgtaatctc agtctgcctt
      961 cctattacag gatcaagaag cttatccctg cacttagctg tcagatcggt tccgaaatct
     1021 tcaagctgct taagcacact ttgcttctgc ttttttcttt cttcatcttc aatattcgat
     1081 tcactggcac tttcaggcac aacttcttct ttcatcatgg cttcggccag ctttttcctc
     1141 atatccgtaa gattaacgcc tgtacgctta agaatatgcg aggcaattcc ttctgcttca
     1201 cgacatatgc caagaagaat atgttctgca tctattcttg aagaacccat aagctcagat
     1261 tcatcatacg ccagttcaat tatgcgttta gaacgcggag taggctgagg tgaacctatg
     1321 attccctgag aagcgttagt tcctacgacc tgggttattt cgtttttaat tttgttatag
     1381 tttaagtcga actgtttgaa aacaagatca agatatcttc cgcccacttt gagaagtccg
     1441 agaagaagat gttctgttcc gacatacgga tgacccatgt ctcttgcttc attctgcgct
     1501 tctataaata ctttggcagc gcgctcggta aagttgtcaa acatattttc acctccatgt
     1561 ctaaaaaaat tatttaacac atatatttta acacgataaa gcttaaaaat caaaaaaaat
     1621 acgtctaaat gatatataat agggatgaaa cacaaaaatt acttaaagga gaaaagatgg
     1681 aactgacaaa gtactctcac aaagaagaaa aatttacgga cgaacttgta aaaatgatat
     1741 actttacaga caaggatatc tcaagatatc tcttttctga tgattatgca aaggctgctt
     1801 acgctataaa aatgatgctt ataaaaaacg agtatatatt tttaaatgac tgtataaata
     1861 ttctgtatga taaaagtgat cttcttgggt tttctctcgc ttactcagga gaagataaaa
     1921 aaaagtttat tcttgacaca gaaatggaaa aaaagataaa ggctctcgaa gagatactga
     1981 catcaaatat ctctgatcgc cagatgtatc tgcattatat ctatactgac aaagccgtga
     2041 gaggcaaagg cgcaggttca atgctcatgc aaaaggtttt ctccgaagca aaggataaag
     2101 aatgtaccga gctactgctg gatgtagctg cagataacag caatgcgata agcttttata
     2161 aacataaggg attttctgtt tatgaggaga aaaaaaatgt aagtataaat ataattggaa
     2221 gcttatgtat gaaaatgaaa atttaaggct taattaaaaa gcaaactctt ttatataaaa
     2281 aagttacaga gaaactcatt atgttataaa aaaaatataa gctaaaatta aaaatccgga
     2341 ggtgagcgta tgagaaaata tataatgttc ataataatag tgattacact acttatacct
     2401 tttagtgccg ctgccagaga caacaaaggg ataataaccg atcctgccaa agaactgaaa
     2461 tggaattatt ttgttgataa tcttctctac gcaagatttt taaatcttca ggaatattcg
     2521 gctctcagag gcttgggact taatgattac gtactcatag ataagcccgg agagatcaac
     2581 gataaactca taggttcctt gagcatagga aacaggatgt ttcccatatt aaattctctt
     2641 accggcataa cagattttta tgacgtgtac tttatgaatg ccagttacat taaagacgaa
     2701 cctgttttga catttttggt aaaaagtttt tttgaggatg atctacctcc cggttatgca
     2761 tggagcctca tagcaaggta tgtaaaagct ctttttcatc tttcttttgt ggatgtagag
     2821 ataaaggtaa atgaaacata cactctagaa ggaaaaaata atataggcga actggttaca
     2881 cgtctatatg caaaaaactc aagcttgtac ccgtttggaa actgggacag gagctatgct
     2941 ccggtcagaa taaacaataa tccttactac actatgatag aacatcttgg tagtattcaa
     3001 aaatcctata actccaacca aaaaactctt tacttgagga atcttttccc gatagccgga
     3061 tggagtacaa actggaacag caatttctca aatccagaag atattattta tcttttccca
     3121 gaacttgaac tctatgctga tttctcaatc cctttcaatc agtcgccggg agattatgtg
     3181 ttggtgcttg atatgcagat ttctccttct ccgtattttt aagttcagca ttccgtaata
     3241 caaaagtaat tgacaaagac atgaagcttt ggtataatct ttttcggtat ttcaagagct
     3301 gtagccattg ccgacgtagc tcaatcggca gagcggctga tttgtaatca gcaggttggg
     3361 ggttcaagtc ccttcgtcgg ctccagtaaa aaaataagat ggtaaggtgc ccgagcggcc
     3421 aaagggggcg gactgtaaat ccgctggcag aatgccttcg aaggttcaaa tccttccctt
     3481 accaccattt tgatatgata aaaaagaatt gaggggtgac aaaaatggca accaaaaatc
     3541 aggtagttca gttttcgcta aagtgttccg aatgtggcac aaggaattat tataagcaga
     3601 agagcagact ttttaagaat aagatagaac tgagcaagta ctgtccaaag tgcaacaaac
     3661 ataccaaaca caacgaatcc aagatataat tcctaaaaag cccttgccaa aagcaggggc
     3721 ttatttgata acggaggtgc cacaatatgt ctaagttctg ggtatttttg tcacaagtat
     3781 ggcaggaagc taaaaaggta tcctggccat caaagaaaga gcttctgaat tcaacggtaa
     3841 tagttcttgc cattcttctc ttttttgcag tctacctttt tgcggttgat ttaggtttga
     3901 tgactttctt caagaacctt atctatccta taaccttcgg aggataattc tataaatata
     3961 aaaaggtgat ctgaatgcga aaagagtggt acatagcgca ggcctattcg ggaatggaaa
     4021 ataaagttaa agaggtaatt gaagaaaaag ccaaaatgct tggcttgagt tctttgtttg
     4081 gaagagttat tgttcctgaa atagaagaga tcgactatgc caaccgaaag gtagacagag
     4141 ttttcatatc caacgaagcg gaactctata ctaaagcttc caagtacgta aaaaaaggtg
     4201 acattctagc caaagaacct tcgatatact gtaaaaaaac cggcgcagta accgaggtaa
     4261 aaaattacag aagaatcatt gttgaaactg tagataaaaa gtactccaaa acttttgtca
     4321 tacctgaaag cgcaggaata atagcaggtc tgaggacggg cagggatatt aagtctggaa
     4381 tgccttttgc aaagtctgct gcttatgagt gtgatgtcga cggtgagata ggaagcattg
     4441 aaaaagttaa aagagtagtt attcaaagta gcttgaacga tgaagaggtt tacgtcattc
     4501 ccgtaaaact atttgattct aagacgttac gaataggtgc aaaaattgat tccggaaaga
     4561 agcttgcaaa cggaagcgag tatgctgcaa agtcttcagg acgggttgat ataaaagaac
     4621 atctcatgag aaaagaaata aaaataatca aaaccaacaa atcaaaactt ttcccaggat
     4681 atgttttcat agaaatgata tacacacagg agtcagaaaa tcttataaaa actgttccgt
     4741 atatttcaac tcttttgaac gtaggaggca aaccgataaa gctcaccttg aaagaggcta
     4801 aggctcttct taagcttgtc ggtcaggagc tcgtcgaaca gaaaaagctt cctaaagttc
     4861 gtatggacta taccataggt gaacagatca agatcataaa cggtcctttt gaacttttca
     4921 ctgggaaaat aaccaaaata aatcttgaaa agcaggaagt aaaagttacc ataactcttt
     4981 tcggaagaga aaccacagtg gaactttctc ttgccgaaat cgaaaagata gagtaaaata
     5041 attaatgtag catttttgat tgtaagtggg agggtaatcc cgctaaaacc acaaggaggt
     5101 cattaaaatg gctaaaaagt tagtcagagt tgtaaaatta cagcttgaag caggaaaagc
     5161 aacaccagca cagccagtcg gtcctgcact cggtcagcac ggtgtaaacc ttatggagtt
     5221 ctgtaaaaag tttaacgctg ccacagctga caaagccgga aacgttatcc ccgtagaaat
     5281 atccatattt gaagacaggt cattcacatt tgttctcaag accccgccag catcgtttct
     5341 cataaaaaaa gcagccggag tcaagacagc cgctaaaaca cccagcaagg aaattgtcgg
     5401 caagattaca tctaagcagg taagagatat agctgagctt aagatgaaag atttaaatgc
     5461 tatgacaatt gaagctgcta tgagaattat tgaaggaact gccagaaaca tgggcataga
     5521 agtagtagaa taagcggggg tggatgaaga tggcaaagca cgggaaaaaa tataatgaag
     5581 ttaaaaaact tgttgacaga accaagtttt atactctgga tgaagctata gaacttgtaa
     5641 aaaagatgtg ttacgcaaag tttgatgaat cagtcgaagt tcacgttcag cttggaattg
     5701 acgcaaccaa gagcgaccag aatgtaagaa gcacagtatc cctgccgcac ggaattggta
     5761 agacagtaag gatagctgtt tttgctaccg gtgacaaagc aaaagaagcc aaggatatgg
     5821 gcgctgattt cgtcggagcc ggcgatctgg ttgaaaaagt tatgaaagaa ggctggacag
     5881 actttgatgt ggccatagct gctcctgata tgatgaaaga tgtcggtaag ctcggtaagg
     5941 ttttgggtcc taaaggactt atgccctcac ctaaagctgg aaccgtcact gcagatatcg
     6001 gcaatgcagt aaaagagttt aaagccggaa gaattgaagt aagaaacgat aaaaccggta
     6061 acgtccattt ccctgtagga aaaaaatcct ttgacgacat aaagataaaa gagaacatcg
     6121 tagccggact tgaacagctt aacaaactta agcctacaac ttccaaaggc aagtttataa
     6181 aaaaggtcac cttagcttca acaatgagtc ctggtgtaaa gcttgacaat tccgtttcgt
     6241 tatagtataa taaccactgg ttgaacaagg acagaaagaa aaagtgccag agacagcagg
     6301 tatttgtata tttaaaggat tattccgcct gcctaggtgc gtgaaaaaga agaaatcgta
     6361 tcttttcgcg acacctgtat ggtgtcgcga tttttattca ggaggtggac gttttgttga
     6421 caaaagagaa aaagtcagct ctgattgatg aaatatcttc agcattaaaa acatccccca
     6481 tagttgtatt taccgatttt tcaggtatgc ctgtagctgc ttcgaacaag atgagaatgg
     6541 aaatgtacaa gcagttcggc gataatgcaa cctatagggt aggaagaaac acgctcataa
     6601 gaacagcagt aaaaaaagca gaactcagtg tccaagaata ttcggcgctt atagacggaa
     6661 gcacaggagt cttttatata aatgattccg tggatcctat agaagcactc aagtttctta
     6721 cggagtttgc aaagaaaaac agcaacaggc cagttgtaaa aggcggaatg cttgaaggtg
     6781 tcatatttga tgccaaacag gcagcagagt acgcaaagct tccttcaaag cagcagctta
     6841 ttgctatggt actgggcagt ttgaatgctc ctatatccgg tttggtcaac gttatggctg
     6901 gaaccctgaa aaaggttctt tatgcgttga atgcagtaaa agaacagaaa gaaaaagcag
     6961 aataaaaata ttatttatta cggaggtgtt ttaaatgaca aaagaagaac ttatccaggc
     7021 tataaagtct atgacagtag cagatttagc agaattggta aaggcattgg aagaagaatt
     7081 tggtgttagc gcatcggcac cagtagcagt agcagcagct cccgcagcag cagcagcagc
     7141 tcctgttgaa gaaaaaacag atttcacagt tgttataaag agctttggcg caaataaagt
     7201 cggagttata aaagttgtca gagaaattac cggtctcgga ctcaaagaag caaaggactt
     7261 agttgaaaaa gccggaactc ctgatgcaaa ggtaaaggaa aatgctccta aggcagaagc
     7321 agaagatata aagaaaaagt tagttgaagc aggagcagaa gtagaactta aatagttttt
     7381 taaaaagaac ctttttgtta aagaaaagtt tataatctaa tggtacaatt taccccgcat
     7441 cgaacttttt cgttcggtgc ggttttgtta tgtaaaatta aaaatacgat ctctaaatta
     7501 taaagtcgag gtgattcata gtggtgaaca cccgctcggt cagtgtggga agaaggagca
     7561 gggaattttt cggtaaagtt catgaagatc attccatgta cgatgatctc ataaaaatac
     7621 agaaagattc tttcaagaac tttgtagaac gcgggatgat tgaatcagta agaaagtaca
     7681 tgcctatatc tgtgcctata aaaaccacag gcaaaaaaac taaggacatc attgttgatt
     7741 ttatcgacat caagtttgaa gaacccggtg ttgatgaaca ggaatgcaag tacaaggatc
     7801 ttactttttc aggaaaggct tatctgaaaa ttcagattac cgactcttcc acgggtgaag
     7861 tacttgaaag agatgatgtt tttctttgca atgtcccctt catgaacgac aggggtattt
     7921 tcatcatcaa cggtgctgaa agagttgttg taaaccagct cgtaaggtca cccggtattt
     7981 attttgtaaa ggaagaagaa aaagattcca gcaaagaact gtttatcgca cattttctgc
     8041 ctgtaaaggg tgcctggttt gagattatct ataatcccaa cgaaggcaag gaagttcttc
     8101 aggttaggat tgacagaaaa agaaagttca attttttcct ttttctaaga gccattggct
     8161 atgaaacaga tctttcaata ctggatcttt tcccaaagga aatagacctg gatgatgaaa
     8221 ttgaacgtga aaagcatctt gatagtgtgg tacttaccga tctctcatac cccgaacttc
     8281 aggacagaga acctgcgctt accaccataa gaggtcttag acttaaggaa gcactcgatc
     8341 ttcttcagaa atacgaagta aaaaaggtta cgttagccaa caatatagct cagataaccc
     8401 ttgacaagat gaagagacga tatgaaaaag atggtgctct gacatctctt gaagcttata
     8461 aggaaatgta cataaaactc aaacctactg aaataccaaa accacagaaa gctaaagaag
     8521 agatagacga catgtatttt tcacatccga atgagcctga aaaagacaag tttgactttt
     8581 ccgaaatagg tcggaaaaaa gcacaggtaa ggcttaagca tgtttacaaa cgctatcttg
     8641 aagaagttga aggcagaacc ttctctttag aggatggaga caaagttgag tataccatca
     8701 actcaaaaac catagacaaa cttgacataa tactggccgc aagacatttg cttgaaatag
     8761 aaagcaatca ggattatctt gataccagag accatctggg caacaaaagg gtaagagccg
     8821 taggagagct tatgcagatt gagtttgaaa aagctttttc caagatgata cagcatattc
     8881 cggaaaaagt catctccatg gcccagtcac tgagcaaaat taacccacag tcccttataa
     8941 attctagagc cataatgacc gctttccacc agttttttgc taccagccag ctttcacagt
     9001 tcatggatca gataaacccc atttcggagc ttacccacaa gagaaggctc tcggcaatag
     9061 gaccagacgg tttgaaaagg gagcatgcga agtttgaggt aagagatgtt caccactctc
     9121 attatggaag aatgtgtcct atagaaactc ctgaaggagc caacataggt ctcatcacct
     9181 cgctttcaat actagcacag actgacgagt ttggattttt gaaaactcct tacttcaggg
     9241 taaaagatgc gatcgttcag tatgcgggag ataaggccag ttctatagtg cctctttcgg
     9301 ctgatgaaga agaaaattat aaaatagccc cggcttcaat tgaaatagat gaaaaaggaa
     9361 atataatacc tccgtatgtt gaggtaagat atcagggaaa ggtgacgttt gttccaaaga
     9421 aagaagtgga ttatatcgct gttaccccca aacagatagc ttcggtatca gcggcactta
     9481 ttccgtttct tgaacacgat gatgcaaaca gagctcttat gggatcaaac atgcaaagac
     9541 aggctgttcc tcttgtatat acggaagccc catgggtcgg aaccggcgtt gagtggttgg
     9601 cggcaagaga ctcaggctat cttatatttg caaagcacaa gggagtcatc gaatatgtcg
     9661 atggaagaaa gatagtgata gccagaactc ttgacgaagg cgaagattgc cggacaaaag
     9721 aaaaagatgg taaacgctac ctcattgaca atgaaggaaa tataataaaa gatgagtacc
     9781 agatactaaa gtatatacgt tcaaaccagg atatgtgtat caatcagcga ccagttgttt
     9841 ccaaaggaca gactgttgaa aaaggagata ttcttgcaga cggaccggcc accgatatgg
     9901 gtgagctcgc act
//
LOCUS       DAOSHR010000070         9832 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_5878_length_9832_cov_4.908356, whole genome shotgun sequence.
ACCESSION   DAOSHR010000070 DAOSHR010000000
VERSION     DAOSHR010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 9832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9832
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5878_length_9832_cov_4.908356"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /locus_tag="PLU28_05260"
     CDS             complement(<1..622)
                     /locus_tag="PLU28_05260"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HOG34508.1"
                     /translation="MTKKTVFLILIFVFALYLFAGYPQKSINLIVPWPVGGITDRVAR
                     ALAPIFEKYIGVPVVVMNIPGASGAIGTEQLYAKPSDGYNILFSAETLGMFRVLGSSK
                     LGFEDFDPISMVIEDEKVIVVPKNSKYMTMDDLIKDIKNKPGKIRMSYSGPGASGHIQ
                     GLLLKKSGLDVSMTPFGGGSPAMLATISGQVEFTVGNVANVLEYIKS"
     gene            complement(704..1873)
                     /locus_tag="PLU28_05265"
     CDS             complement(704..1873)
                     /locus_tag="PLU28_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34509.1"
                     /translation="MKLKIITFVVILVALVSFAVPQMDWGRVYQMPGIQEIFYVKATN
                     DGFYAIGSSNETGNNENAIALKFDKFGNLVSKSEFGGAYSDWALWAFEDSRKDMVLVG
                     ATNSFSDGFDYYLAKVSPVYWETYIPKLGNDKASAGVDVEDGYIIVGYAQDPYDKNNS
                     NYKGYMVKIDKDAGNVIWYRWLSGADTFKDVKPLGIEKVSDGFVISGVVFDYDTGISS
                     LYVVKTDFEANEIWSKVFTGQSYGRAFEVKEFNDEYYVVGYTGDLNGAGFSDIYVVKF
                     SWKGDLLWEKTFGQKGSDHGYSIKISKNGNMYVGGYITNPKTNNVDAVLLVYDKDGNE
                     IYNKAIGGKGRDVVYSLDITEDGDIYMVGYSDSKDFGADLSDVLIIKFSDILMHK"
     gene            complement(2075..3916)
                     /gene="dnaK"
                     /locus_tag="PLU28_05270"
     CDS             complement(2075..3916)
                     /gene="dnaK"
                     /locus_tag="PLU28_05270"
                     /inference="COORDINATES: protein motif:HMM:NF001413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HOG34510.1"
                     /translation="MADKEYVVGIDLGTTFSAIAWMKPDGNVEVIPNAEGKRITPSIV
                     SFGKDGEIIVGEPAKRQAILNAEKTVRSIKRYMGTDHLVKIDSKNYTPQEISAFILKK
                     MVKDAESYLDGKIKKAVITCPAYFNDSQRQATKEAGEIAGLQVLRIINEPTASAVAFG
                     LDKTSGDKKIVVYDLGGGTFDVSVLEIGDGIVEVKATSGNNHLGGDDFDQRVIDWLIN
                     EFKNETKIDLRNDKQAFQRLKDAAETAKIELSTKFEAEINLPFITVVNGTPMHLERKL
                     TRSKFEELVRDLVESTRGPVENAIKDSGFSFNEIDEILLVGGSTRVPMVQKLIKDYFG
                     KEPNKSVNPDEAVAVGAAIQSAIMTGKTDKDIVLVDVTPLSLGIEVKGGLMQVIIPRN
                     SKIPLKKSNIFTTAADFQTEVEVGVFQGERSIAKDNFLLGSFKLTGIAPAPRGVPQIE
                     VTFDIDSNGMVNVSAKDLGTQKLQSMVVTGRQKLDPDQIQKMINDSKVYEEQDKRRRE
                     EVELKNQGDELAYQIDKVLSENGDKIPSDLKNSLESKVKELREALSQDNIGKIKILID
                     DIKNESMKVGQHLYQNQQGQQSNPGDQTSSGSDDTAGNSNNETASNN"
     gene            4250..5071
                     /locus_tag="PLU28_05275"
     CDS             4250..5071
                     /locus_tag="PLU28_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOG34511.1"
                     /translation="MFVDFHSHSTFSDGTLTPCELVKMANDKKIQMFSITDHDNVDGQ
                     LEAFSAAKSFGIDYVAGVEISCEYPTMLDMLGYGYSVGNNFLVETLEELKKYRINRNT
                     LMIEKLNGLGIEITLEEVAAQAGGDVIGRPHFARVMQNKGYVSTKEEAFEKFLGDDRP
                     GYVRKKRLTPQAAIELISIAGGVSVLAHPKYLNLEKTRFENLVKHLKDFGLWGIEVFY
                     SKNTFSENVFFEEIALKYDLFITAGSDYHGANSMDVSLGMKVDAEKIVKTMQYFR"
     gene            5093..5824
                     /locus_tag="PLU28_05280"
     CDS             5093..5824
                     /locus_tag="PLU28_05280"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HOG34512.1"
                     /translation="MKSSDYPVIVLGHRGYRKLYPENTLLAFKKAVEFGADGVELDVW
                     KAKDGSIVVIHDPDCERVSGLRGIVKELSYKEIRKLRLPMNQYIPTLDMVYAELGEDV
                     FINVEIKDFEAVETSLQIVRSFNAVDRTLFSSFIPEALYRLRELQSDAYLGLLVDTKD
                     QLSDISKVNERVGLYSLNAPLDGIKFFGVEAFKNLIAKVKEEDIKVALWHFDEAEWVE
                     KIKGYYDCIITDDVQAIVNKLEEIY"
     gene            5837..6892
                     /locus_tag="PLU28_05285"
     CDS             5837..6892
                     /locus_tag="PLU28_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/ornithine racemase family PLP-dependent
                     enzyme"
                     /protein_id="HOG34513.1"
                     /translation="MYPRLNIQKESIAKNASYLASFCSRNGISIAGVTKVLCGSLELA
                     EIFLKNGVKIIADSRVKNLDRIKSYFPQAYTMLIRIPMISELRQAVSVSDCILVSDIH
                     TLTVISEVSREKGKIPDIVYMVDMGDLREGVWFEQAVQEIRTACSIGNVNLLGIGTNL
                     GCFGGVLPTVENMRKLVEIKEKINVSTIRDISGGSTVTLKMLEDGVLPKEITNLRIGE
                     AIVCGTDATGGRNVPNTLQDTVLLEAEIIEIKDKPSVPYGETGYDAFGRKHEFIDYGI
                     RKKAILAIGEQDVSSSGMTPLDKNIKVLHSSSDHTIIDITDCEENYCIGDIIDFKLSY
                     GALLRASTSPYVEKIFK"
     gene            complement(6906..7613)
                     /locus_tag="PLU28_05290"
     CDS             complement(6906..7613)
                     /locus_tag="PLU28_05290"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HOG34514.1"
                     /translation="MPNVFLLDSFNGEYACLNEEECAHLRSVRVRENEVINVTDGKGK
                     LFECIVTKINKKTADLKILKIKNIHCSFLPEINLFIGASKWDRMQILIEKAVEHRVSS
                     IFVFNGEKSTFKYDDQKKFLKLIAESSKQSFLCNFPKFSFINIKDIPLKNTLVFDFQG
                     TSSLKEELKKKLNSVNIVFGPDTGFSQNEKKFFHETFFSVVNLGNTVFRFETAALYVL
                     SSINYEYERLLKNYPLE"
     gene            complement(7618..8892)
                     /gene="serS"
                     /locus_tag="PLU28_05295"
     CDS             complement(7618..8892)
                     /gene="serS"
                     /locus_tag="PLU28_05295"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOG34515.1"
                     /translation="MLDMKFVRTNIEKIRNCLINRNSSTLILDELVALDEKRRDILKK
                     VEDIRSLKNTNSKAVARLKAQGDEEALRQNIENGKILNQQSEELEAQLKQIDEQYMQR
                     LLYIPNVYDEDTPIGKDETFNPEIRRIGIPRKFDFEPKPHWEIGSELNGLDFDRGSKL
                     SGSRFTIMKGDFARLERSLGAFMMDLHSTEHGYTEVMPPHMVLRETITATGQLPKFEE
                     DLYNTKGDDLFLIPTSEVALVGMHRDEILELKDLPAKYCGYSPCYRREAGSYGKDVRG
                     VIRQHQFDKVELVWHTTPERSADDLETLTSHAEKVLKLLELPYRVIRLCSGDLGFGAS
                     RTYDIEVWLPSYNSYKEISSCSNDGDFQARRGNIRFRDKDNKLKYVHTLNGSGLAIGR
                     TLVAIVENYQTSDGKIKVPDVLIPYMKKEYIG"
     gene            complement(8912..9535)
                     /locus_tag="PLU28_05300"
     CDS             complement(8912..9535)
                     /locus_tag="PLU28_05300"
                     /inference="COORDINATES: protein motif:HMM:NF015901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HOG34516.1"
                     /translation="MKKITLFFIILLTISIVFAQSQISVSSGFGTLGDKQHTLKDNVI
                     IKKSDLTMMTEEATITQLDDDWRDMVCSATTIKTSTLNASAEFFKFDLSTERGQLSKK
                     VSVKIVVDKKDLYISCDVLEFNNKEKNYAGSSNDSVKILKEDYVISAKNFSYNETTKI
                     LVLTGDVWIKNDVKKIDLKTQNSTFRTDTNEMKAEGINLTLEVKETK"
     gene            complement(9532..>9832)
                     /locus_tag="PLU28_05305"
     CDS             complement(9532..>9832)
                     /locus_tag="PLU28_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34517.1"
                     /translation="ELSDMFLIPSSYICLSPYNLSPDGLIISYEAGGSVFKAMNAAEI
                     LLKISYARTRELFRIISKDIELITCESPLEDPLDSVAFYDGMKTCQKYMEGKLEL"
BASE COUNT         3022 a         1788 c         1698 g         3324 t
ORIGIN      
        1 ctgatttaat atattctaga acatttgcaa catttcctac agtaaattca acctgtccgg
       61 aaatagttgc aagcattgcc ggacttcctc ctccaaaagg agtcattgaa acatcaagac
      121 cagatttttt aagaagaaga ccctgtatat gtccgcttgc accaggtcct gagtaagaca
      181 tacgaatttt tcctggcttg ttttttatat ctttaatcag atcatccatg gtcatgtatt
      241 ttgaattttt ggggacaaca ataacctttt catcttcaat aaccatagaa attgggtcaa
      301 aatcctcaaa accaagttta ctagagccta agactctgaa cataccgaga gtctctgctg
      361 aaaataaaat attatagcca tcagaaggct tggcataaag ctgttctgta cctatagctc
      421 ctgatgcacc agggatattc atcacaacaa ctggtacacc gatatacttt tcaaatattg
      481 gagccaaagc tcttgcaact ctgtcggtta ttccaccaac aggccatgga acaataagat
      541 taatggattt ttgaggatat cctgcaaaca gatacagtgc aaatacaaag ataagtatca
      601 aaaatacggt ctttttagtc atattttcgt tcaatcctcc aatataagtt ttatacaaag
      661 aaaaacaagg ggataacccc ttgtcataat tagaatacta atattatttg tgcatcagaa
      721 tatcagagaa tttaatgata agaacatcac ttaagtctgc cccaaaatct tttgaatcag
      781 aatagccaac catataaata tctccgtcct ctgttatgtc caaagaatag acaacatctc
      841 ttcctttgcc gcctatagct ttgttataga tctcattgcc atctttatcg tatactaaaa
      901 gaaccgcatc aacattattg gttttgggat tagtaatgta accgcctacg tacatatttc
      961 cattcttact tatctttatt gaataaccat ggtcagagcc tttctggcca aatgttttct
     1021 cccacaaaag atcacctttc caggaaaact taactacgta aatgtcagaa aaacctgcac
     1081 cattaagatc tccagtataa ccgacaacat aatattcatc gttaaattct tttacttcaa
     1141 aagccctgcc ataactctga cctgtgaaaa cctttgacca tatttcattt gcttcgaagt
     1201 cagtttttac tacataaaga cttgatatac cagtatcata atcaaacaca actccagaaa
     1261 tgacaaatcc gtcagatact ttttctatac ctaaaggctt cacatcttta aaagtatccg
     1321 ctcctgaaag ccatctgtac catattacat ttcctgcatc tttgtctatt tttaccatgt
     1381 aacctttgta gttagaattg tttttgtcgt aaggatcctg agcatatcca acaataatat
     1441 aaccatcctc tacgtccact cctgcgctag ctttatcgtt ccctaatttg ggaatgtatg
     1501 tctcccagta aacagggctt acttttgcga gatagtaatc aaagccatca gaaaaagagt
     1561 ttgttgcacc aactaatacc atatctttcc ttgaatcttc aaaagcccat aaagcccaat
     1621 ctgaatatgc tcctccaaac tcacttttag aaacaagatt tccaaattta tcgaatttca
     1681 acgctatggc attctcatta ttacctgtct cattagaaga acctattgca taaaatccgt
     1741 catttgttgc tttcacataa aatatttcct gaattccagg catttgatat actctgcccc
     1801 aatccatttg cggaactgca aaagaaacta aggcaactaa aataacaaca aaagttataa
     1861 tctttagttt cacatttatc cctcccatat gtattaaaaa tatcttattt taagcaatag
     1921 aatactttct catatattat accacaaata atacagtttg attataaaat tcaaaaaata
     1981 agtatatgta aactaaaaaa gactgtatgt aaaacttaat tatattttac catacttttt
     2041 tattaaaaca aaagggcgga tagccgccct tttgttaatt attactagct gtttcgttat
     2101 tggagtttcc tgctgtatca tctgagcctg aagaggtttg atcgcctggg tttgactgct
     2161 gaccctgttg attttgatat aaatgctggc caaccttcat ggactcattt tttatatcat
     2221 caatcaaaat ctttattttt cctatgttat cctgagaaag agcctctctt agttctttga
     2281 cttttgattc aagactattt tttaagtcag aaggaatctt atctccattt tcgcttaaaa
     2341 ccttatctat ctgataagca agctcatcac cctggttttt gagttcaact tcttctcttc
     2401 ttcgtttatc ctgttcttca tatacttttg aatcgtttat catcttttga atctgatcag
     2461 gatcaagttt ctgtcttcct gtaactacca tagactggag tttctgagta cctaaatctt
     2521 ttgcggaaac attaaccatt ccatttgaat cgatatcgaa agtaacttca atctgtggaa
     2581 cacctcttgg tgcaggagca atacctgtca gtttaaagct gccaagcaga aaattatcct
     2641 ttgcaataga acgttctccc tggaagactc ctacttcaac ttcagtctga aaatcggctg
     2701 ctgttgtaaa aatattactt tttttaagcg gaatcttact gtttcttgga attataacct
     2761 gcattagacc accttttact tcaataccta acgaaagagg agtaacatca accaacacaa
     2821 tatctttatc agtctttccg gtcataattg cagactgaat ggctgcgccc acagcaactg
     2881 cttcatcggg attaacactc ttgttcggct cttttccgaa ataatccttt ataagtttct
     2941 gaaccatagg tactctggtt gaacctccaa ccaaaagaat ttcgtcaatt tcattaaacg
     3001 aaaaacctga atcctttatg gcattctcaa caggtcccct tgtggattct actagatctc
     3061 tgactaactc ttcaaactta cttctggtta gtttcctttc cagatgcata ggtgtgccat
     3121 ttacaactgt aataaaagga agatttattt ctgcttcaaa ctttgtagaa agttcaattt
     3181 ttgcagtttc agcagcatct ttaagtctct ggaaagcctg tttgtcatta cgcaggtcta
     3241 ttttggtttc atttttaaac tcatttataa gccagtctat taccctttgg tcaaaatcgt
     3301 ctccgccaag atgattattt ccggaagtag cttttacttc tactattccg tctcctattt
     3361 caagaaccga gacatcaaag gtaccgcctc caaggtcata cacaactatc tttttgtctc
     3421 cggaagtttt atcaagacca aatgccactg ctgaagcagt aggttcattt attattctca
     3481 gaacctgaag accagcaatt tcaccagcct cctttgtagc ctgcctttga gaatcattaa
     3541 aataagccgg gcaggttata accgcttttt ttattttacc gtcaagataa ctttcggcat
     3601 ctttaaccat ttttttcagt ataaatgcac ttatttcctg tggtgtatag ttttttgaat
     3661 caatctttac cagatgatct gtacccatat atcttttaat tgaacggact gttttttcgg
     3721 cgttaagtat tgcctgtctt ttcgctggtt caccgactat tatttcaccg tcttttccaa
     3781 aagatactat tgagggagtt attcttttgc cttctgcgtt tggtattact tcaacgttgc
     3841 cgtctggttt catccaggct attgcagaaa aagttgttcc aaggtctata cctactacat
     3901 attccttatc agccatataa agtacctcct ttatgcacat aaaaaatttt agaaattttc
     3961 agattaagat gtttctgaag aagccatttt taatcttctg aatttattat acttcaaaaa
     4021 tcataagata tcaagttgtt ttttaaaaat ataaaaaaac ttatacctat aatatatact
     4081 ggtatataag tatactaaat attttattta taaaaatgta tatcactcgt tactatagag
     4141 tgagaaaaaa gaaaaaacta ttttctgcaa caatgttaaa aaagtaattg taattaatat
     4201 cgtaattaag gtataattaa tatatatata attttttgga ggatctattt tgtttgttga
     4261 ttttcactcg catagtactt tttctgatgg aacacttact ccttgtgaac ttgtaaagat
     4321 ggcaaatgat aaaaaaattc agatgttttc tattaccgac catgataatg ttgatggtca
     4381 gctggaggct ttttcagcag caaagtcctt tggaatcgat tatgttgctg gagtagagat
     4441 aagttgtgaa tatccaacga tgcttgatat gcttggttat ggctattcag tggggaataa
     4501 ttttttggtt gaaacgttgg aagaacttaa aaaataccgt atcaacagaa atacgttgat
     4561 gattgagaaa cttaatggct taggtattga gattacgctt gaagaagttg cagctcaggc
     4621 gggtggtgat gtaattggaa gaccgcattt tgcaagggta atgcagaata aagggtatgt
     4681 tagtaccaag gaagaggctt ttgagaagtt tttaggcgat gataggccag gatatgtaag
     4741 aaagaagagg ttaactcctc aagcagccat agagcttata tcaattgctg gtggagtttc
     4801 tgttttagct catccaaaat atctcaatct tgaaaaaaca agatttgaaa acttggtaaa
     4861 acatttgaaa gactttggat tatggggaat agaagttttc tattcgaaaa atactttttc
     4921 tgagaatgtt ttttttgagg aaattgcctt aaaatatgat cttttcatta cagcaggcag
     4981 tgattatcat ggtgctaaca gtatggatgt atctttaggc atgaaggttg atgcagaaaa
     5041 aattgtgaag acgatgcaat attttagata aaaactttta ggggggctca atatgaaaag
     5101 ttctgattat ccggttatcg ttcttggaca cagaggatat agaaagctat atccagaaaa
     5161 tacgttatta gcttttaaaa aagcggtaga gtttggtgca gatggtgttg agcttgatgt
     5221 ctggaaggca aaggacggaa gtatagtggt gatacatgat cctgactgtg aaagagtatc
     5281 ggggctcaga ggtattgtaa aggaactttc atataaagag ataagaaagc ttcgtcttcc
     5341 gatgaaccag tatattccca ctctggatat ggtatatgca gaacttgggg aagatgtgtt
     5401 tataaatgtt gagataaaag attttgaggc tgttgaaact tcccttcaga tagtaagaag
     5461 ctttaatgcc gttgatagaa ctttattttc atcttttatt ccagaggcac tttacagatt
     5521 aagagaactt cagagtgatg cttatcttgg acttcttgta gataccaagg atcagctttc
     5581 ggatatatca aaagtaaatg aaagagtggg tctgtactct ttaaatgctc ctttggacgg
     5641 tataaagttt ttcggagtgg aagcttttaa gaatcttatt gcaaaggtga aggaagaaga
     5701 tataaaagtc gctttatggc attttgatga ggccgaatgg gtagaaaaga taaagggata
     5761 ctatgactgc ataataacag acgatgtgca ggcaatagta aataagcttg aagaaattta
     5821 ctgaccgggg tgttttatgt atccaaggct taatatacaa aaagagagta ttgctaaaaa
     5881 tgcctcttat ttagcttctt tttgcagtag aaacggtatt tctattgctg gagtaaccaa
     5941 agttctctgc ggaagcttag agcttgcaga aattttttta aaaaacggtg ttaagattat
     6001 agctgattcg agagtgaaaa atcttgatag aataaaatcg tattttccgc aggcgtatac
     6061 tatgcttata aggattccaa tgatatcaga actcagacag gcagtatctg tttccgattg
     6121 tattcttgta agtgatattc atacgttaac tgttatatca gaagtttctc gtgagaaagg
     6181 aaaaattccc gatatcgttt atatggtgga tatgggagat ttaagggaag gagtctggtt
     6241 tgaacaggct gtacaagaaa tcagaacagc ctgtagtatc gggaatgtaa atcttttagg
     6301 tataggtact aacttaggat gttttggcgg agttttgccg acggttgaaa atatgaggaa
     6361 gcttgttgaa ataaaagaaa aaatcaatgt gagtactatt agagatattt cgggagggag
     6421 tacggttact ctcaaaatgc ttgaagatgg tgtgcttccg aaagaaataa ccaatcttcg
     6481 tataggtgaa gcgattgtat gcggtacaga tgcaactggc ggaagaaatg ttcccaatac
     6541 ccttcaggat acagtattac tggaggctga gataatagag atcaaagata aaccctctgt
     6601 accttacggt gaaacgggct atgatgcatt tggaagaaag catgagttta tagactatgg
     6661 aattagaaaa aaagccatac tagctatagg tgagcaggat gttagttctt caggaatgac
     6721 tcctttggat aaaaacatta aggttctaca ttccagcagt gaccatacga ttattgatat
     6781 cacagactgt gaagagaact attgcatagg cgatattatt gattttaagt tatcgtacgg
     6841 agctcttctg cgtgcaagca caagtcctta tgttgaaaaa atattcaaat agaaactatc
     6901 cccttttatt caaggggata gttttttagt agtctttcat actcgtaatt tatggaactt
     6961 aacacataaa gtgcagcagt ttcaaatcta aaaacagtat ttcccaaatt cactacagaa
     7021 aaaaaggttt catggaagaa ttttttttca ttttgggaaa agcctgtatc ggggccaaaa
     7081 actatattca ctgaattcaa cttcttttta agttcttctt ttaaagagct tgttccttgg
     7141 aaatcaaaaa caagtgtatt ttttaaagga atatccttta tgtttataaa agaaaactta
     7201 ggaaaattac ataaaaaaga ttgttttgag ctttctgcaa tcaacttcag aaattttttt
     7261 tgatcatcgt acttaaaagt agacttttca ccgttaaaaa cgaatattga acttactctg
     7321 tgttccacag ctttttcaat aagtatttgc atcctgtccc atttcgaagc accaataaaa
     7381 aggtttattt ccggaagaaa ggaacaatgt atattcttta tcttgagtat tttgagatcg
     7441 gctgttttct tgtttatttt tgtcacgata cactcaaaaa gcttaccttt cccatcagtt
     7501 acattgatta cttcattttc ccttacccgt acggatcgca gatgagcaca ttcttcttcg
     7561 tttaggcaag catactctcc gttgaatgaa tcaagcaaga aaacattagg catactttta
     7621 tcctatatac tcttttttca tatatggaat aagtacatct ggaactttga ttttcccatc
     7681 agaagtctga taattttcaa ctattgccac gagagttcgt ccgatagcaa gacctgaacc
     7741 gttaagagtg tgaacgtact taagtttgtt atctttatcc ctgaacctta tatttcccct
     7801 gcgtgcctga aaatcgccgt cattagaaca tgaggatatt tctttataag aattatagct
     7861 tggtaaccat acttcaatat cgtaagttct gctagcgcca aatccaagat ctccgctaca
     7921 caaacgtatt actctgtatg gaagttcaag caattttaaa actttttcgg catgcgatgt
     7981 aagagtttcg agatcatcgg ctgatctttc aggagttgta tgccatacaa gctcaacttt
     8041 atcgaactga tgctggcgta ttactcctct tacatcttta ccgtagcttc ctgcctcacg
     8101 tctgtaacat ggagaataac cgcagtactt tgctggcaga tccttgagtt caagtatctc
     8161 gtccctgtgc atgcctacta atgccacttc agatgtagga ataagaaaaa ggtcgtcacc
     8221 ttttgtgtta taaaggtctt cctcaaactt tggaagctgc ccagtagctg ttatggtttc
     8281 tcttaagacc atatgtggag gcattacctc ggtatagcca tgttctgttg agtgaagatc
     8341 catcatgaat gctccaagtg atctttctag tctagcaaaa tcgcctttca taattgtaaa
     8401 cctagatcct gaaagcttgg aacctctgtc aaaatcaaga ccgttcaatt cactgccaat
     8461 ttcccagtgt ggtttgggct caaaatcaaa ttttcttggg ataccaattc ttctgatttc
     8521 tgggttgaat gtttcatcct ttccaatggg agtatcctca tcgtatacat taggaatata
     8581 aagaagtctc tgcatgtact gctcatcaat ctgtttcaac tgagcttcca attcttcaga
     8641 ctgctgatta agaatttttc cgttttcaat attctgacgg agtgcttctt catccccctg
     8701 tgcttttaac cttgcaactg ctttggagtt tgtattttta agagatcgta tatcttcaac
     8761 ttttttgagg atgtcccttc ttttttcatc aagagcaact agctcatcaa gaatcaaggt
     8821 tgaagaattt ctgtttataa ggcagtttcg tatcttttca atgttagttc ttacaaactt
     8881 catatcaagc ataaataagt cctcctaaat attatttggt ttcttttact tccagtgtca
     8941 ggtttattcc ttctgctttc atttcatttg tatcagttct gaaagtcgag ttctgtgttt
     9001 taagatctat ttttttgaca tcgtttttta tccatacgtc tccggtaagt acaagaatct
     9061 tggtagtttc attgtaagaa aagttttttg ctgaaatcac ataatcttct tttaatatct
     9121 tgactgaatc atttgaggaa ccagcataat tcttttcttt attattaaac tcaagaacgt
     9181 cacatgagat gtaaagatct tttttatcaa ctacaatctt tacagatact ttttttgaaa
     9241 gctgtcctct ttctgtgctt agatcaaatt taaagaattc tgctgaagcg tttaaggttg
     9301 atgtttttat ggttgttgct gaacatacca tatcgcgcca atcatcgtcc aactgggtaa
     9361 tagttgcttc ttctgtcatc atggttagat ctgatttttt tattataacg ttatctttta
     9421 aggtatgttg cttatcaccc aaagttccga atccggagct gaccgaaatc tggctttgag
     9481 caaaaacaat acttatagtt aataagataa taaaaaatag agtaattttc ttcataattc
     9541 aagttttccc tccatatatt tttgacacgt tttcattcca tcataaaagg ctacactatc
     9601 caatggatct tccaatggac tttcacaagt tattagttca atatctttgc ttatgatcct
     9661 gaacaattct cttgtacgtg cgtaagaaat ttttaaaagt atctcggcag cattcattgc
     9721 tttgaataca gagccacccg cttcataaga aattatcagt ccgtctggag agagattata
     9781 tggtgaaaga cagatatatg aagaaggaat taaaaacata tcggatagtt ca
//
LOCUS       DAOSHR010000071         9409 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_6205_length_9409_cov_5.943019, whole genome shotgun sequence.
ACCESSION   DAOSHR010000071 DAOSHR010000000
VERSION     DAOSHR010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 9409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9409
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6205_length_9409_cov_5.943019"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /gene="wecB"
                     /locus_tag="PLU28_05310"
     CDS             complement(<1..823)
                     /gene="wecB"
                     /locus_tag="PLU28_05310"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HOG34518.1"
                     /translation="MKVISLVGARPQFVKEAVINWEFRKQGITEILVHSGQHYDYNMS
                     DIFFKSLDIKSPDYNLNVGSATHGQMTGRILEAFEAIVIKEKPDMVLVYGDTNTTLAG
                     AIASVKLKVPIAHVEAGIRQKPKDMPEEINRVLTDRISNFLFCPSMLSVKNLDCEGIK
                     DGVEFVGDTMYDVYTKMEKLFDTSQALKEYGLEKNGFVLATIHRDFNTDNPEKLSQII
                     DGLNRLSKEIKVLFPIHPRTKKLATTLGKKMLFDVTDPIGYLQLQALVKASRLVVT"
     gene            complement(972..1045)
                     /locus_tag="PLU28_05315"
     tRNA            complement(972..1045)
                     /locus_tag="PLU28_05315"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1011..1013),aa:Gln,seq:ctg)
     gene            1189..2499
                     /gene="rpoD"
                     /locus_tag="PLU28_05320"
     CDS             1189..2499
                     /gene="rpoD"
                     /locus_tag="PLU28_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HOG34519.1"
                     /translation="MAKLQTGDKKNNLSKKSSRISKKESIEIIGQIPELEVKNINPDS
                     QQAEVKVIVRSRKNVDAIIQTLEKTAKSKNGILTYSDIDEAMPVEVSDELDTELIEKI
                     YVALEEAGIQILDDEDSEEEEEISDVLDDTRTEEEPADDYPDFYDDIELKMYDNISLN
                     DPIKIYLKEISKVPLLTPARERLLAKKAQKDDKKARDELIRSNLRLVISIAKRHVGRG
                     LSFLDLIQEGNIGLIKAVNKFDWRRGFKFSTYATWWIRQAITRAIADQARTIRIPVHM
                     VETINRLNKVIREYLQENGEYPSVQELSKVMGKSEEKVNEILLSARDIISLNSPLTSG
                     SGEDDDSETGDFVGSQDLTPEEEAQKMILKEKLEEVLDTLHPKEALVLKMRYGFLDGK
                     QKTLEEVGQFFNVTRERIRQIETKALRKLRHPSRSSQLKDIIDN"
     gene            2675..4006
                     /locus_tag="PLU28_05325"
     CDS             2675..4006
                     /locus_tag="PLU28_05325"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOG34520.1"
                     /translation="MTSKLCKFLDIGILFLINILSGVPVLVSLFIAFVLFVGIYSFRT
                     YDYDNMKNFIQNTIRVFMGVVSGFAMLAVFYYFLRDYVSAESVFYTFVFATVIIPVVN
                     RISFALYIKTVIPRKYLVIGKKNEIGHILDEITRKSYGKLLFVEYINPSPAKLHKLIE
                     ENYKKRDFSPFTQFFYPDEKPWKEGTFDSIIITDPILEKFIQNDLEKLKDKGMDIEYL
                     PTVAEFYLKRIPVEVLEKFEEHYKIAFGSISNSPAKRMLDFIVSLVALIILSPILAVV
                     ALSILIENGRPVVFKQPRTGKDNEVFIMHKFRSMRNQSEEDVKGKFATDEKSRILKIG
                     RIIRMFRLDETLQFWDILTGEMSLIGPRPEQPHFVSQYEKIIPFYSYRHKLKTGLTGW
                     AQIMYKYTSTVEETKVKLSYDLYYVKNRTSILDINIILKTIEAVIWRRGAV"
     gene            4023..5876
                     /locus_tag="PLU28_05330"
     CDS             4023..5876
                     /locus_tag="PLU28_05330"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HOG34521.1"
                     /translation="MVREADYSIKDVLYIFKRRKMVFLLVFAAVMAGVFLFLFMAPKK
                     YEAFMILQYNNAEDSRSYVSSSLASLLGSGGKSGENGIPNEIQKMKSDDILGILVDKY
                     DLVNSTNNSPSLYMRLIGKKVNKRDMIDSMKKDIVITYHRPDDIVENTSLLRISYKSS
                     DRNFAYSVVKTLYEEYLKKDLIGYQKKSQDNIEKIYALYSQYSSEYEDISKQIIKFRM
                     DNKISSDPSSFANNSLFEYYGQLYSKILTLDERKTQIDISLKSIEDNFIKADRETKEL
                     LLMNDSTIASTKRSIIENKIKLETLRKNSPNATQIPLLESEIETQEEFLQELINNYFK
                     SNEKLLLGMDYNTYKEYISLKIEKENQSVLKEVYASIMKKLELSINQQSKTYEQLFDL
                     SKKQAENELKYKSALKVLESEKIKSASYATSFTVMDDVFVPEREIYPSKKSTVLLGFV
                     LAVFAAMAVSLTVDFNDKKVHDLVMFSKTVARVDFIISKKRRKIEELKVLNSIKTSEK
                     NIVGITYAPGIKIHEHIDEMRKILRETSLNFTDLSGLSEKDTYSAYDLLDSNVKSVYL
                     MDECNGWKTSLFSGKCDCIYILVKEKSSLVSDFARFYNDFDESKIKIVYLG"
     gene            5880..6932
                     /locus_tag="PLU28_05335"
     CDS             5880..6932
                     /locus_tag="PLU28_05335"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOG34522.1"
                     /translation="MKKVLIIITKGDWAGAQRVVYEIAKYIKLNYSDTLELDVAIGIK
                     GPLNEELKSIGVRVIELNNLIWEVSLSSDTKAVREIRRLIEENQYDVVHVHSTKAAFV
                     GRIAARKANVKNIIYTVHGWWGIERFSGIKRYMVSRLEKYCAGFTDHMVFICKRDMSF
                     ALKNKIGPSGAYNLILNDVSIDSTVEPSLRKTFGISDKTTVIGNIGRLDDTKQPQAFV
                     DIAQEVLKKNKDYFFVWIGSGLVKPVIEPQYKNKIKFVGFMKNPYPYLRDFDLLLMTS
                     KYEGMPITIIEALKYNIPVLSSNVGGITEYLPSKNLYMNSNEAIGKILKKNYEKNIVY
                     SDPDMAKKYAELYLNG"
     gene            6925..8094
                     /locus_tag="PLU28_05340"
     CDS             6925..8094
                     /locus_tag="PLU28_05340"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOG34523.1"
                     /translation="MVKLNSRTIQYYLLILYTFFSGVVFFEPSVGDILFIVLLPILII
                     HVKFKKTEFSLLALLLVPALFSFFWGFVKFGFLNFKFVFIDIYLFLLFVFFKVCLDNF
                     YEYDRNIIDTLMKTWLAVAYLNVFTGLFSYATGRFNIMGQSIVGFGIRLIGFFKDPNV
                     FGPFACVCGLYVFEKFYQSNKNLIFAIFNFGFMSLGVFLSFSRAAWLNYFAGLLFIVL
                     VYSFKNKNMYKWRTLLLVFLLVFFMVMLIFSDITIAGISFKEFFKGRLGIKSYDAQRF
                     ASQGKSLVMLDMSKIFGIGPGNYERITNYSAHNTYLRYMGERGFLGLITGGFLIAFIV
                     IRALKIKGKYFFLAASLIGLLVNSYFVDTLHWRHLWIVFAMIVSYSYRKEPYGES"
     gene            8081..9358
                     /locus_tag="PLU28_05345"
     CDS             8081..9358
                     /locus_tag="PLU28_05345"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOG34524.1"
                     /translation="MGNLKTVIISAYSPKDTEIAALRIEKLAKFLSCYYETYVISGRP
                     RREKKFFDTAKAKLIETRFKSYAAENLAVSSTVVQNTSAKKINKFNLYYFLEYFFPIA
                     PGGMRFYKKRDFFSEFEKLMLSFDNNEKVLVIVSYGPHFILKIGRSLKKKYKDRIILV
                     SDFRDPYRNFESQNDSFKFMHKSLKKILAYSDLITTVSSIMKERLCVNYSVREEKVHV
                     LYNGYDREDFNFSKIMPQEKSNIDIVFTGSFYPEDFLQIDPFLKALSENKNKNMVNFI
                     YCGKDSDYVRNSFEKYGLSQRLTDMGMLDRKSSLELQNQSDCLLLVTYTGNDPDQGSW
                     RISGKVYEYLQNHIPILNIGSPKWELRDILQSDGVSKVIPAYDTKGISEFIDMAVQKK
                     LKPDFAAREKLLEEFSYERLIEKFVQKIESLKL"
BASE COUNT         3043 a         1493 c         1939 g         2934 t
ORIGIN      
        1 cggttacaac cagcctgctt gcctttacaa gagcttgtag ctgaaggtag cctatcgggt
       61 cggttacatc aaaaagcatt ttctttccca gagtcgtggc cagtttcttt gttctgggat
      121 gtataggaaa cagtactttt atttcttttg aaagcctgtt taaaccgtcg attatctgag
      181 aaagtttttc agggttatcc gtgttgaaat ctctgtgtat agtcgcaaga acaaagccgt
      241 ttttttcaag accgtactct ttaagagctt gtgaggtatc aaaaagtttt tccatttttg
      301 tgtatacatc atacatggtg tcaccgacaa actcaactcc atcttttatt ccctcacagt
      361 caaggttttt cacggaaagc atgctgggac agaaaagaaa gtttgaaatc ctgtctgtaa
      421 gtaccctgtt tatttcttcc ggcatgtctt taggtttctg tcttatcccg gcttctacgt
      481 gggcaatcgg aactttgagt tttactgatg caattgctcc tgcaagtgtg gtgttggtgt
      541 ctccgtagac cagaaccatg tccggttttt cttttattac tattgcttca aaggcttcaa
      601 ggattctgcc tgtcatctgt ccgtgtgtgg cagagcctac attcaggtta taatcaggtg
      661 attttatgtc taggctctta aaaaatatat ctgacatatt gtaatcatag tgttgtcctg
      721 aatgaacaag tatttctgtt attccttgtt ttctgaactc ccaatttatg acagcctctt
      781 ttacaaactg cggccgtgcg cctacaagag atatgacctt catttttttc tcctttggat
      841 aaatagtatt tcgaatttta atccgacaac agatttatta tatcatgatt ttgacgtttg
      901 acatacctgc atgcctgtgt tagaatatag ggtgaaaaaa gaagaacctt ttcaggttct
      961 tctttgatgt ttggctggga gaggcggatt cgaaccacca tagtcggatc cagagtccga
     1021 agtcctgccg ttggacgatc tcccaaagcg tacatgcaaa attataacac aaacaaccat
     1081 gtctgtcaat aaaaatattt gaactttttt ttaattttta agtctaataa ttttggtgct
     1141 tcatttatga aactcttcaa aatttctgca acacaagggg gtaataagat ggcaaagctt
     1201 cagaccggcg acaaaaagaa caacctctca aagaaatctt cccgtataag caaaaaagaa
     1261 agcattgaga tcattggcca gatccccgaa ctggaagtaa aaaatataaa tccggattct
     1321 cagcaggcag aagtaaaggt tattgtgaga agcagaaaaa atgttgatgc cattatacag
     1381 actctggaaa aaacggcaaa atctaaaaac ggaatactta cttattccga tatagacgag
     1441 gctatgcctg tggaagtatc tgatgagctt gatacggaat taatagaaaa gatatatgta
     1501 gcacttgaag aggcgggtat acagatactt gacgatgagg actccgaaga agaagaagaa
     1561 atatcggatg ttcttgacga tactagaacc gaggaagaac cggcagatga ttatcctgat
     1621 ttttacgatg atatagagct taaaatgtat gataatatat cgctgaatga tcctataaag
     1681 atttatctta aggaaataag caaggttcca ctcctcaccc ctgcgcgcga gagacttctg
     1741 gcaaaaaaag cccaaaagga cgataaaaag gcaagagatg agcttatacg ttcaaacctc
     1801 cgtctggtaa taagcatagc aaaacgtcat gtaggaagag gtttgagttt tcttgacctt
     1861 atccaggaag gaaacatagg tcttataaag gcagttaaca aattcgattg gcgcagaggc
     1921 tttaagtttt caacgtatgc aacatggtgg ataaggcagg ctattaccag ggctatagcc
     1981 gatcaggcta ggactataag aattccagtt catatggttg agaccataaa caggcttaat
     2041 aaggtgataa gggagtattt gcaggaaaat ggtgaatatc caagtgttca ggaactttcc
     2101 aaagtaatgg gaaagtctga ggagaaggta aacgaaatac ttctgagcgc cagagatatc
     2161 atctctctca actctccttt aacaagcggg agcggcgagg atgacgattc agagaccggt
     2221 gattttgtgg gttctcagga tcttactccg gaggaagagg cccaaaaaat gattcttaaa
     2281 gaaaaacttg aggaggttct tgataccctt catccaaaag aagcgttggt tctcaagatg
     2341 agatacggat ttcttgacgg aaagcaaaag actcttgaag aggttggaca gtttttcaac
     2401 gttacacgtg aacgtataag gcagattgaa actaaagctc tgagaaagct caggcatcct
     2461 agccgtagtt cgcagcttaa agatataatt gataattaac caagtcataa aaggcatatg
     2521 ttaaaaacat cccgcttttg ccgaactaaa aatccggcaa aagttttttt gtatgtaaca
     2581 tgttagtgta attttcatgt tgttccgacg aaaggatatt tatttgggat attatgtgat
     2641 aaaatatatg taattattgt tttatggagg ttttatgaca agtaaactct gtaagtttct
     2701 tgatataggt atcttatttc tgataaatat tttatccgga gtacctgtgt tagtttcttt
     2761 atttattgcc tttgtgcttt ttgtaggcat atattctttc cggacgtatg attatgataa
     2821 tatgaaaaac tttatacaga acaccatacg tgtatttatg ggggttgtgt caggttttgc
     2881 catgcttgcg gttttttatt acttcttgcg ggattatgtt tcggcggaat cggttttcta
     2941 tacatttgtt tttgcaactg ttatcatacc tgtagttaac agaataagtt ttgctctgta
     3001 tataaagaca gtcataccgc gcaaatatct tgttatagga aagaagaacg agataggtca
     3061 tatacttgat gaaataacca gaaaatcata tggaaaactc ttgtttgtag agtatattaa
     3121 cccttctcct gcaaagcttc ataagcttat tgaagaaaat tataaaaaga gagatttttc
     3181 tccttttacc cagttttttt atccggatga aaagccttgg aaagagggaa catttgattc
     3241 gataataatc actgatccga ttttggaaaa gtttatacag aacgatttgg aaaagcttaa
     3301 ggataaggga atggacatag aatatcttcc gactgttgca gagttttatc ttaaacgcat
     3361 cccggttgag gttcttgaaa agtttgagga gcactataag attgcatttg gaagcatttc
     3421 aaattcaccc gcaaaaagaa tgcttgattt cattgtttcg cttgtagcgc ttataatcct
     3481 gtctccgatt cttgcagtgg tggccttgtc catacttatt gaaaatggaa ggccggtggt
     3541 ttttaaacag ccaagaaccg gcaaggacaa tgaagttttt ataatgcata aattcagaag
     3601 catgaggaat caaagcgagg aagatgtcaa agggaagttt gccacagacg agaaaagccg
     3661 tatacttaaa attggaagaa taataaggat gttcagactt gatgaaactc ttcagttttg
     3721 ggatattctt accggagaaa tgtctcttat aggtccaaga ccagagcagc ctcattttgt
     3781 gagtcagtat gaaaagatta ttccatttta ttcatacagg cataagctta aaacaggtct
     3841 tacaggttgg gctcagataa tgtacaagta tacatccacc gtcgaggaaa ccaaagtaaa
     3901 gcttagttat gacctttatt atgtaaaaaa caggacttcg atacttgata taaatattat
     3961 tcttaaaacc atagaagctg ttatctggcg cagaggggca gtataaaacg gagggataga
     4021 ttttggtaag agaagcagat tattccataa aagatgttct atatatattt aagcgtagga
     4081 aaatggtttt tttacttgtg tttgcagcgg ttatggcagg agtgtttctt ttccttttta
     4141 tggctcctaa aaaatatgaa gctttcatga ttttacagta caacaatgcc gaagatagca
     4201 ggtcatatgt ttcaagcagt cttgcatcac ttcttggttc tggtggaaag agcggagaaa
     4261 acggtatacc caatgaaata caaaaaatga aaagtgatga tatactcgga atacttgtgg
     4321 ataagtatga tcttgtaaac agcacgaaca acagtccaag tctttatatg agacttatag
     4381 gaaaaaaagt caacaaaaga gatatgatag attctatgaa aaaagatata gtcataacct
     4441 accacagacc tgatgatatc gttgaaaata cctcgctttt gagaatatcg tacaaatctt
     4501 cggacagaaa ttttgcgtat agtgttgtaa aaacattgta tgaagagtat ttaaaaaaag
     4561 atcttatagg atatcagaaa aaatctcagg acaatattga aaaaatatat gccctttatt
     4621 cgcagtacag cagtgaatat gaagatataa gcaagcagat aataaaattc aggatggata
     4681 acaagatatc gtctgatccg tctagttttg ccaacaacag cctttttgaa tactatggac
     4741 agctttattc gaaaattctg actttggacg aaaggaaaac acagatagat ataagtctta
     4801 agagtattga ggataacttc ataaaagcgg acagagagac caaagagctt ctgcttatga
     4861 acgattccac tattgcttct actaaaagaa gtataatcga gaacaagata aaacttgaaa
     4921 ctcttcggaa aaactctccc aatgctaccc agatacctct tcttgaatca gagatagaaa
     4981 cccaggagga gtttcttcag gagcttataa acaattattt taaaagcaac gaaaaacttc
     5041 ttctaggcat ggattacaat acttataaag agtacataag tcttaaaata gaaaaggaaa
     5101 atcagtcagt actgaaagaa gtctatgctt ctataatgaa gaagctcgaa ctgagtataa
     5161 accagcaatc caaaacgtac gaacagcttt ttgatctttc aaaaaaacag gctgaaaacg
     5221 agcttaaata caagtctgca cttaaagttc tggaaagtga aaaaataaaa tctgcttctt
     5281 atgctaccag ctttacggta atggatgatg tttttgttcc ggaaagggag atatatccca
     5341 gcaagaaaag tacagttctt ttagggtttg tgttggcagt ttttgctgcc atggcagtga
     5401 gtcttactgt ggattttaat gataaaaaag tacatgatct tgtgatgttt tcaaaaactg
     5461 ttgcaagggt agatttcatt ataagtaaga aaagaagaaa aatagaagag cttaaggttc
     5521 tgaacagtat taagacttct gaaaaaaata tagtaggcat tacttatgct cctggtataa
     5581 aaattcatga acatatcgat gaaatgcgta aaatcctcag agagacatct ctgaatttta
     5641 cagacctttc cggactttct gaaaaggata cttatagtgc atatgatttg ttggattcaa
     5701 atgtaaaaag tgtttatctt atggacgagt gcaatggctg gaaaacttct ttgttcagcg
     5761 gaaaatgtga ctgtatatat attctggtga aggagaaatc ttctttggta tctgactttg
     5821 caaggtttta taacgatttt gatgaatcga agataaagat cgtctacctg gggtgatgta
     5881 tgaaaaaagt tcttattata ataaccaagg gtgactgggc aggagcgcaa agagttgttt
     5941 atgagattgc aaaatatatc aaactcaact attccgatac gctcgagctt gatgtagcta
     6001 taggaataaa aggacctctt aatgaagagc tcaaaagcat aggtgtaagg gttattgagc
     6061 ttaataatct tatatgggag gtttcgttaa gtagcgatac caaggctgta agagaaatcc
     6121 gtaggcttat tgaagaaaat cagtatgatg ttgtccatgt ccatagtacc aaagcggcct
     6181 ttgtaggaag gatagccgca aggaaagcca atgtaaagaa tataatatat actgtccacg
     6241 gatggtgggg aatagaaaga ttttccggga taaaacgtta tatggtaagc cgtttagaaa
     6301 agtattgtgc cggtttcacc gatcatatgg tttttatctg caaacgggat atgagttttg
     6361 cattgaaaaa taaaataggt ccaagcggtg catataacct tatattaaac gatgtttcta
     6421 tagacagtac agtagagccg tctctgagga aaacctttgg aataagtgat aaaacgactg
     6481 taatcgggaa tatcggaagg cttgatgata ccaaacagcc tcaggctttt gttgatatag
     6541 cccaggaggt tttaaaaaag aataaagact atttttttgt ctggataggt tcaggactcg
     6601 ttaagccggt aatagaaccg cagtacaaga ataagataaa atttgtagga tttatgaaaa
     6661 atccgtatcc ttacttaagg gattttgatc ttcttctaat gacctccaag tatgaaggaa
     6721 tgcccataac cataatagaa gcattaaagt ataatattcc ggttctttct tctaatgtgg
     6781 gagggataac cgaatatcta ccttccaaaa atctttatat gaacagcaat gaggcaatcg
     6841 gaaagatact taaaaaaaac tatgagaaaa atatagttta ttccgatcct gatatggcaa
     6901 aaaaatatgc ggagctgtat ctgaatggtt aaactgaatt caagaaccat tcaatattac
     6961 ttacttattc tttatacatt tttttccgga gttgtttttt ttgaaccgtc ggttggggat
     7021 atacttttta ttgttcttct tccaatactt attattcatg taaaatttaa gaaaacagag
     7081 ttctcattgc tggcgcttct attggttccg gcattgtttt cttttttctg gggatttgta
     7141 aagtttgggt ttttgaactt taaatttgtt tttatcgata tatatctgtt tcttctcttt
     7201 gtttttttca aagtctgtct tgacaacttt tatgagtacg accgtaatat tatcgataca
     7261 cttatgaaaa catggcttgc ggttgcatat cttaatgttt tcaccggact tttttcttat
     7321 gctactggaa gatttaatat aatgggtcag agcatagtgg gttttggaat aagactgata
     7381 ggatttttta aagatccgaa tgttttcgga ccttttgcat gtgtctgcgg cctttatgtc
     7441 tttgaaaaat tctatcaaag caacaaaaac ttgatctttg ctatatttaa ttttggcttc
     7501 atgagcctag gggttttttt aagtttttcc cgcgcagcat ggcttaatta ctttgcgggg
     7561 ttgcttttca tagttcttgt atattcgttc aaaaataaaa atatgtataa atggagaacg
     7621 cttcttctgg tttttcttct ggtgtttttt atggttatgc ttattttttc ggatataacc
     7681 atagcaggaa tcagtttcaa agagtttttt aaaggtcgtc tgggaataaa aagttatgat
     7741 gctcagagat ttgcttcgca gggtaagtcc ctggtgatgc ttgatatgtc aaaaattttt
     7801 gggataggtc ctggcaatta tgaaaggata actaactatt ctgcacataa tacatatctt
     7861 agatatatgg gagagcgtgg gtttttagga cttataaccg gcggtttcct gatagctttc
     7921 attgttataa gagcgttaaa gattaaggga aagtattttt tccttgctgc ctcgcttata
     7981 ggactgcttg taaactctta ctttgtggat actttacatt ggcggcattt gtggatagtc
     8041 tttgccatga tagtatccta ttcttacaga aaggagccgt atggggaatc ttaaaaccgt
     8101 tattataagt gcatattcgc ctaaagacac tgaaatagca gctttgcgta tagaaaagct
     8161 tgccaagttc ctttcctgtt actatgaaac atacgttata agcggcaggc cccgcaggga
     8221 aaagaaattt tttgatactg caaaggctaa gcttatagaa acacgtttca aatcgtatgc
     8281 tgctgagaat cttgcagtat cttccactgt tgtacaaaat acttctgcaa aaaaaattaa
     8341 taaatttaat ctttactact ttcttgaata tttctttcct atagcgccag gaggcatgag
     8401 gttctataag aagagggatt ttttttcaga gtttgaaaaa cttatgcttt cttttgataa
     8461 taatgaaaaa gttttagtta tagtttctta cggaccgcac tttatattga aaataggacg
     8521 cagtctgaaa aaaaagtata aagacaggat aattctggta agcgatttta gagatcctta
     8581 cagaaacttt gagtcacaga acgacagctt taagttcatg cataaaagcc taaaaaaaat
     8641 tcttgcatac tcagatctta taacaacagt ttcatccata atgaaagaaa gactctgtgt
     8701 caattactcg gtaagggaag aaaaggtaca tgtgctttat aacggatatg accgggaaga
     8761 ttttaatttt tcaaaaatca tgccgcagga aaaaagcaac attgatattg tttttaccgg
     8821 aagtttttat cctgaggatt ttttgcagat agatcctttt ctcaaggctc tttcggaaaa
     8881 taaaaataaa aatatggtaa actttatata ctgtggaaaa gactctgatt atgtcagaaa
     8941 cagttttgaa aagtacggtc tttcacagag gcttaccgat atgggaatgc tggataggaa
     9001 gagttctttg gagcttcaga atcaaagtga ttgtctgctt cttgtaactt atacaggaaa
     9061 tgatccggat caggggtcat ggcgtatatc aggcaaggtt tatgagtatc tgcaaaatca
     9121 tattcctata cttaacatag gaagtcctaa gtgggagctg cgcgatatac tccagtccga
     9181 cggagtaagc aaggtaatac cggcttacga tacaaaagga atatctgagt tcatcgatat
     9241 ggcagttcag aagaagttaa aacctgactt tgcagccaga gaaaagttgt tggaggagtt
     9301 ttcttatgag cggcttatag agaagtttgt tcaaaaaata gagagcttaa aattataagg
     9361 gaggacatat gtgcggaata tacgggtgtt tgggcgaaac atccatgca
//
LOCUS       DAOSHR010000072         9359 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_6259_length_9359_cov_5.550408, whole genome shotgun sequence.
ACCESSION   DAOSHR010000072 DAOSHR010000000
VERSION     DAOSHR010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 9359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9359
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6259_length_9359_cov_5.550408"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1926)
                     /locus_tag="PLU28_05350"
     CDS             complement(<1..1926)
                     /locus_tag="PLU28_05350"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOG34525.1"
                     /translation="MKLISLEINGFKSFANNTALDLSKNIIAIVGPNGSGKSNIVDAI
                     RWLLGEQSSRQIRISEKTDVIFNGTDEQKPSSKASVFLSMTDSQDKKISVGRVLERNG
                     SSVYYINNKPSLLKDIYRVFENRGVGKQFYSIISQGQVSEIVNSSPEAIMKIVLDAAE
                     IGEYIEKKNSSLLLLSKTSENLERLNDVLFEMDKRIKSLSNRASRAQKYLKYKQELNL
                     YAKEFLGSKKIQLIEQKEMTATEIKDMELKLNSFLSQLFETERKYRALKEEIESTDKE
                     LSKHGELIETYRTRLSSLEEQKNTLNQKKYEINSNIMNLTMKIEVLDEQIKNNSEKAE
                     VLSAQEKAVEAELEVLVSELAVLQAHADETEKKLQDDERHLNEVISKHEELKATINAD
                     EQSINSSSVRLSSLQERMTILSHEKESTIALIEKMEEYLHEIENTVHKDNKEELTARN
                     AISEKESETDKISAELSNTNRTLYELMKDSENVKYKIELIKNQISDYDGYMPAVKSFI
                     KNFRNDDNVIDVAANLFEIDQAFEQAVSSSVGHKLQNVVIKSSSKSQEYVDYIKKIGE
                     GKITFLPLDLINAKVSYKRSVTNEPGVLGYMIDLCRYDQKYKKIMEYLFLNTLVVDDI
                     KTAIKISKTDFSSSIVTL"
     gene            complement(1928..2584)
                     /locus_tag="PLU28_05355"
     CDS             complement(1928..2584)
                     /locus_tag="PLU28_05355"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOG34526.1"
                     /translation="MESTKERDFNLIKEKIENCQKCPLALTRNKTVCGEGNIDSPVIF
                     IGEGPGAEEDASGRPFVGRAGQLLEKMILMYAKRKREDFFIGNIVKCRPPQNRVPSEE
                     EVRSCTHFLEAQLLVIKPKIIVTLGATSARFFLGRDIQITKERGSFFDWYGNTKIFTT
                     FHPSYLLRNQSKEKFSPFWYAALDMTLIGIMHQQLSENRPVDEVINEITIKLKGSTGE
                     "
     gene            complement(2596..5142)
                     /locus_tag="PLU28_05360"
     CDS             complement(2596..5142)
                     /locus_tag="PLU28_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34527.1"
                     /translation="MHLRYKSFSPVLFIISLFFIILSGTKVFSATAYIDAVGFTFSET
                     SVTLEGSVTIKYKDSLAYADRAIAYLNDEDDVSYAQLFGNVVYKFEKHTLQASEMTIN
                     FEKDDSFFINSYAVEKYKTKNKNEVNVKIWSANTNKPIDKDYIISKDVKITTCQDCVT
                     YFLIANKVTVYPDKFLIARNVVMTLFNVPVFYFPFYFQNLSEEDKSPFKVDIKYENDK
                     IGVGVNINNVFENKSFLNYKQVFTNDLKNHKLTQTSSFKYGMPIGPGNLFLNAELTDT
                     SFNKFETAYEFITKATPSATEVSKLSYTYQPASSAETLYFYIPTYVTPYGELRNITSF
                     LNWTNRNFNNATFLKLNSQAFQLKYKTSSISLSSLKIDLSSTESNVPIDKAWQSKRAS
                     VGLDGKYYFYSGETSLSGKYSYNLSAIKNELKTDTFDIKNLLLNQIKLFSFRNGPFYL
                     NSGLKTTVEFDYINMLRVPAQKTYLSKTQLEGAVAPSLNAELWIFNFGLKALELKKVL
                     VNSYTPASTDSFSYNDYIGYKLSLFDGFLYSSMRFDRQFELKDILQDTAMSNIYLFPQ
                     RVFSRITDNSSLTALSVETENSLNLFGTSNNLKTVTKMDLSDKNNFYPDLTNIKLTAK
                     FNFGFTHLSEMDYKHSRDELDRLRESFQNFDIFTMPQKLYDFLKPHSTFVTNTETLNF
                     NRNYLSSKYKLYFGKESFQDMIPESLTEYVFYISGNKIFGKLETKDKSNYIDFTLKFL
                     EQNSLYSFGIGLNYNVKNEYLKSTFILESFDQKEFLTATMTYDLENNILDITSLKLQK
                     KIICWTVYSDIYFKMIPDFQIKEMNIKFFINDIPEKNIEAGTKGIKFNMF"
     gene            complement(5147..6262)
                     /locus_tag="PLU28_05365"
     CDS             complement(5147..6262)
                     /locus_tag="PLU28_05365"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOG34528.1"
                     /translation="MKRLIKYISKEFFGPFLMGIFGFVIFVSVELLYQLSDKIVRNKV
                     GFDKLLLLIWYNLPYFISMGIPVGVLFAIFWIISRLSNDNEIIAFQTLGIPTKRLVIP
                     FIIIGVLLSCFTFLLFDTIVPTANYEAKSAMAKYIYQRAEALIEENQFVDAGDGRYLF
                     VKQVDKENGILYNLLLYEVSYDNVQLFHAQKAQKSEDKWYMDDVRAYKVDDNGFMALD
                     MSFKKLELNINKNIEEFLNFAKTANDMTTRELKQKIDTFSKLGEDVSYLIVSYQEKYA
                     NSLAPLVISLLGVCLSLFLNLKSKSWSVISTFVLVVLYQGSGAWLGALGKEKIINPVY
                     APWIPNLIFGLSGIILFFLLDTRLSYKITEPLKRLLG"
     gene            complement(6259..7020)
                     /locus_tag="PLU28_05370"
     CDS             complement(6259..7020)
                     /locus_tag="PLU28_05370"
                     /inference="COORDINATES: protein motif:HMM:NF014010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HOG34529.1"
                     /translation="MKEKKYIQENPLTPYLREPLIVYLNNVLRMGRIVQGMFYKFSDA
                     YFQKDIPLSEEIISQDDRVDYLEAKLDYEGMVLLSKANFSGIYLKANLLGLKLVYIFE
                     NMADLCESNAKLNIELMKYPPKINVLSFEDLFKQTQEAFSSSLRVFSDFININLITLK
                     NEEFSSVFFNPCQNICTLNNEASSLFHAYKKYLYKSKESIESISLHMDILSNIEHFSD
                     MSTNIAENIVFAITGNKYRCRKNSLDLFFSVEEEE"
     gene            complement(7017..7841)
                     /locus_tag="PLU28_05375"
     CDS             complement(7017..7841)
                     /locus_tag="PLU28_05375"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HOG34530.1"
                     /translation="MKVIIFYNPTKTDVRYIHDNIKNEFENFSIEITDAVQACKDCFV
                     EQAQDNDYFIVLGGDGTVLRTVPLAVAFNKPIIGINMGKLGFLTAFSQNEIRTAAECI
                     SMKKLSFSERMLLEADFNGTKYFCLNDLNVQRSTPDGTIDISVSYMDEPVYSFSGDGI
                     LISTPTGSTGYGLSAGGAILDPSIRAMELIPLAAHGLNMRPLIFGPEKNLSIRMNNAL
                     NEKGFVLVDGNIVSSLKKGESVHVRVSSKTLFLAQKEKYSFLELLNQKLAFGRRFE"
     gene            complement(7847..8143)
                     /locus_tag="PLU28_05380"
     CDS             complement(7847..8143)
                     /locus_tag="PLU28_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HOG34531.1"
                     /translation="MFFIANFFYGTAFVLRIGINFLQISIIISAVLSFILPYYNPVRS
                     FFDSISNFICRPIRRVIPLTFGPIDFTPYLALLLLIFVDKFLIASLFDLSVIMR"
     gene            complement(8159..8851)
                     /locus_tag="PLU28_05385"
     CDS             complement(8159..8851)
                     /locus_tag="PLU28_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOG34532.1"
                     /translation="MDSQLIKNYLNLKNEIAELSEKYSRNSNEIKLIAVSKTFPSADI
                     LECFEKGQRDFGENWAQELREKSSELNLEGLMWHFIGSIQTNKLKYIVPCSHMIHSVC
                     RVQEVIEIDKRAYSLGKVQNILIEVNVSGEESKSGISLSQAQDFIENALKYPNIKVCG
                     LMTMAPNTSDTECISSVFSSLRKLRDNLSLKYPDIKELSMGMSGDYNLAIENGSTMLR
                     IGSKIFGSRIYT"
     gene            complement(8808..9089)
                     /locus_tag="PLU28_05390"
     CDS             complement(8808..9089)
                     /locus_tag="PLU28_05390"
                     /inference="COORDINATES: protein motif:HMM:NF017317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction DNA helicase RuvB C-terminal
                     domain-containing protein"
                     /protein_id="HOG34533.1"
                     /translation="MVRKLLNTVTQNYKGGPVGVKALAASLGIEPDSISEVYEPYLLQ
                     NGYLIRTSRGRMITEKGLRYLGYSESKINSMRGDQIGQSVNQKLPEFEE"
     gene            complement(9090..>9359)
                     /locus_tag="PLU28_05395"
     CDS             complement(9090..>9359)
                     /locus_tag="PLU28_05395"
                     /inference="COORDINATES: protein motif:HMM:NF037140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOG34534.1"
                     /translation="RFGIILEMSFYTDDDLKLIVCRSAELLKAAISEEAALLIAQRSR
                     GTPRIANRLLKRVRDTAQVKERSRIEVSEVQEIMNMLDIRPYLDN"
BASE COUNT         2809 a         1719 c         1680 g         3151 t
ORIGIN      
        1 tagagtcaca atagagctag aaaaatctgt tttcgatatt tttatggctg tttttatatc
       61 gtcaaccacc aaagtattga ggaaaagata ctccattatc ttcttgtact tctgatcata
      121 tcggcacagg tctatcatat atcccaaaac tccaggttcg ttggtaaccg atcgtttata
      181 cgatactttg gcattgataa ggtcaagagg gaggaatgtg atctttccct cacctatctt
      241 ttttatataa tcgacatatt cctgagactt tgaagagctc ttgatgacaa cattttgaag
      301 cttatgtcct acagacgagg aaacggcctg ctcaaaggcc tggtctattt caaaaagatt
      361 ggctgcaaca tcgataacat tatcgtcatt tctaaaattt tttataaagc ttttgaccgc
      421 tggcatataa ccgtcataat cagaaatttg attttttata agctctattt tatactttac
      481 attttcagaa tccttcatga gttcataaag agttctattg gtgttagaaa gttctgcaga
      541 gatcttatcg gtttctgatt ccttttctga tatggcattt cttgctgtaa gttcttcttt
      601 attgtcctta tggacggtat tttctatctc atggagatac tcttccatct tttctataag
      661 cgctattgtg ctttcctttt catgcgaaag tatggtcatg cgttcctgaa ggctgctaag
      721 ccttacagat gaagagttta tactctgttc atcagcattt atagttgctt taagctcttc
      781 atgttttgag ataacttcat taaggtgtct ttcatcatct tgcaattttt tttctgtttc
      841 atcggcatgg gcttgcaaaa cagcaagttc tgataccagc acttccagtt ctgcttcaac
      901 agctttttcc tgagcggaaa gaacctcagc tttttcggaa ttgtttttaa tctgttcgtc
      961 cagaacttct atcttcatgg ttaagttcat tatattagaa ttaatctcat attttttttg
     1021 attcagggtg tttttttgtt cttccagact tgaaagtctt gttctgtaag tttcaataag
     1081 ctctccgtgt tttgaaagct ctttatctgt actttcgatt tcctctttaa gtgcacggta
     1141 ctttctttct gtctcaaaaa gctgtgaaag aaaggagttg agcttgagtt ccatgtcttt
     1201 tatttccgtg gctgtcattt ctttttgctc aataagctga atcttttttg aaccgagaaa
     1261 ctcttttgca tatagattta attcttgttt gtatttgaga tacttttgtg cccttgaagc
     1321 tctgttggag agagacttta tccttttgtc catctcaaag aggacatcat tgagtctttc
     1381 aaggttttca gatgtttttg acaatagaag cagtgagctg tttttctttt cgatgtattc
     1441 gcctatttct gcagcatcaa gcactatctt cataatagct tccggtgagg agtttactat
     1501 ttcacttacc tgtccctgac ttattataga ataaaactgc tttcctacgc ccctgttttc
     1561 aaaaactctg tatatgtctt taagaagaga aggcttgtta tttatgtaat aaaccgagga
     1621 accgtttctt tccagcactc ttccgactga aattttttta tcctgactgt cggtcatact
     1681 aagaaaaaca ctggcttttg aagatggttt ctgttcatct gtaccgttaa agataacatc
     1741 agttttttca gatattctta tctgccttga agactgttct cccaggagcc aacggatagc
     1801 atccacaata ttagattttc cggatccgtt tggtccgact atggctatta tatttttact
     1861 taggtcaagt gctgtattat tggcaaagct tttaaatcca ttgatttcga gggagataag
     1921 cttcaaatta ctctcctgtg cttcctttca gctttatagt tatttcattt ataacctcat
     1981 ctaccggtct gttttcagac agctgctgat gcattatgcc aataagcgtc atatccagag
     2041 cggcatacca aaatggtgaa aatttctctt tcgattgatt tcttagtaag tagcttggat
     2101 gaaaagttgt aaatatcttt gtatttccgt accagtcaaa aaaagatccg cgttccttgg
     2161 ttatctgaat atctcttccc aaaaaaaatc tagctgatgt tgcccctaag gttactataa
     2221 tttttggttt tataaccagc agctgcgctt caagaaagtg tgtacaggaa cgaacctctt
     2281 cttccgaagg tacacggttt tgaggaggtc tgcactttac tatgtttcct ataaagaagt
     2341 cttctctttt tcttttggca tacatcagaa tcattttttc aagcagttgc ccggctcttc
     2401 ctacaaaagg tcttccgctt gcatcttctt cagctccagg tccttcacct ataaagatta
     2461 ccggagaatc aatatttcct tctccacata cagttttatt gcgagttagt gcaagcgggc
     2521 atttttggca gttttcaatt ttttctttta ttaggttaaa gtctctttct tttgtgcttt
     2581 ccaaagtttt ccctcctaga acatattgaa ctttatccct ttggttccgg cttctatatt
     2641 tttttcggga atatcgttta taaagaattt tatgttcatt tctttgattt gaaagtcagg
     2701 aatcattttg aagtatatgt ctgaatagac cgtccagcag ataatctttt tctgcaattt
     2761 aagggaggta atgtccaaaa tattgttttc aagatcatat gtcattgtag cagttaaaaa
     2821 ttccttttgg tcaaaggatt ccaggataaa ggtggatttg aggtactcat ttttaacgtt
     2881 gtaattaagt cctataccaa aagaataaag ggagttctgt tcaagaaact tgagtgtaaa
     2941 atcaatatag tttgacttgt ctttggtttc gagttttcca aagatcttgt ttccggatat
     3001 ataaaaaaca tattccgtca gtgactcggg aatcatatcc tgaaaactct cttttccaaa
     3061 atatagctta tactttgaag aaagatagtt acggttgaag ttgagagttt cagtattggt
     3121 tacgaatgtg gaatgaggtt ttagaaaatc ataaagcttt tgaggcatag taaatatatc
     3181 aaagttttga aagctttccc taagtctgtc aagttcatct ctggaatgct tataatccat
     3241 ttctgaaaga tgtgtgaaac caaaattaaa ttttgcagtg agctttatgt ttgtaagatc
     3301 cggataaaaa ttattcttat ctgaaagatc catctttgta actgttttta agttattgga
     3361 agtaccaaat aagttaagag aattctctgt ttcaacagag agagccgtaa ggctggagtt
     3421 gtcggttatt cttgaaaaaa ctctctgagg aaaaagatat atgtttgaca tagccgtatc
     3481 ctgaagaata tctttcagct caaactgcct gtcaaaacgc atggagctgt ataaaaaacc
     3541 atcaaacaat gagagcttgt aacctatgta atcattataa gaaaaagaat ctgtagatgc
     3601 aggtgtatag gagtttacta aaaccttttt cagttccagg gcctttaaac caaagttgaa
     3661 gatccatagt tcagcattga gagaaggagc tacagctcct tcaagctgag tcttagataa
     3721 gtaggttttt tgggcaggaa ctctaagcat atttatgtaa tcaaattcca cagtggtctt
     3781 tagaccgctg tttagataga aaggaccgtt acggaaagag aaaagcttta tttgattgag
     3841 caagagattc ttaatatcga aagtgtctgt tttcaactcg ttttttatag ccgacaggtt
     3901 atacgagtac tttccggaaa ggctggtttc tccggaataa aaataatact ttccgtccag
     3961 tcccacagaa gcacgtttgg actgccatgc cttatctatg ggaacattgc tttctgtact
     4021 tgaaaggtct attttcaaag atgaaagaga gatgcttgat gttttatatt taagctgaaa
     4081 cgcctgagaa ttaagtttta gaaaggttgc attattgaaa ttacggttcg tccagttcaa
     4141 aaaagatgta atattacgaa gctcaccata cggagttaca tatgtaggaa tataaaaata
     4201 aagggtctcc gccgaagatg caggctggta tgtgtaactc aactttgaaa cctcggtggc
     4261 gctaggagtt gctttggtaa taaattcata agctgtttca aacttattaa aagaggtgtc
     4321 ggtgagctcg gcattcagaa aaaggttgcc cggtcctatc ggcataccgt acttgaaaga
     4381 agatgtctga gtgagcttat ggttttttaa gtcgtttgta aatacctgtt tatagttaag
     4441 aaagctttta ttctcaaata cattatttat attgacacca acacctatct tatcgttttc
     4501 atactttata tcaactttga aaggtgattt atcttcttcg ctgagatttt gaaaataaaa
     4561 gggaaagtaa aagaccggaa cattaaacag ggtcataaca acgttcctag caataagaaa
     4621 cttatcagga tacaccgtca ctttattggc tataagaaaa taggtgacac aatcctgaca
     4681 ggtagtgatt ttaacgtctt ttgagattat atagtcctta tcgatgggtt tattggtatt
     4741 ggctgaccat atctttacat taacttcgtt cttgttttta gtcttatact tttcaaccgc
     4801 ataggagttt ataaagaaag aatcatcttt ttcaaaattt atagtcattt ctgaggcttg
     4861 taaagtatgt ttctcaaatt tgtagacaac gtttccaaaa agctgtgcat aggaaacatc
     4921 gtcttcatca ttgagataag ctatagctct gtctgcataa gccaaagaat ccttgtactt
     4981 tatggttacc gatccttcaa gtgtgacaga agtttcggag aaagtaaaac ccacagcatc
     5041 aatatatgct gtagctgaga atacttttgt tccggatagt ataataaaaa acaaagaaat
     5101 tataaagaga actggtgaaa atgatttata tcttaagtgc atggtatcat cccagcaaac
     5161 gtttcagagg ttctgtaatt ttatacgata agcgtgtatc aagcagaaag aaaagtatta
     5221 ttccgcttaa tccgaagatc aagttcggaa tccatggtgc atatacggga ttaatgattt
     5281 tttcttttcc aagtgcgccc aaccatgctc ctgatccctg ataaagcacg acaagcacaa
     5341 atgtggagat tacactccaa gatttgcttt tcaggttcag aaacaatgac agacaaactc
     5401 caagcaatga tatcacaaga ggagctaagg agtttgcata cttttcttga taagatacaa
     5461 tcaaataaga cacatcctct ccaagttttg aaaaagtatc aatcttctgc ttcaactctc
     5521 ttgtagtcat atcgtttgct gtttttgcaa agttaagaaa ttcttcaata ttcttgttta
     5581 tattgagttc aagctttttg aaagacatat ccaaagccat aaagccatta tcgtcgacct
     5641 tgtatgccct cacatcgtcc atgtaccatt tatcttcgct tttttgagcc ttctgagcat
     5701 gaaaaagctg aacattgtcg taacttactt cgtaaagaag aagattatag agtataccat
     5761 tttccttgtc tacttgtttt acaaaaagat atcttccgtc tccggcatcg acaaactgat
     5821 tttcttctat cagtgcttca gccctctgat atatgtactt ggccattgcg gacttagctt
     5881 cataattggc ggtcggaact atggtatcaa ataacagaaa ggtaaagcat gagagtaaca
     5941 ctccgattat aataaaagga ataacaagtc ttttggtagg tataccaaga gtctgaaaag
     6001 ctatgatctc attgtcgttt gaaagtctgg agattatcca gaatattgca aaaaggacgc
     6061 ctacaggtat acccatcgat ataaagtacg gtaaattata ccagatgaga agaagaagct
     6121 tatcaaatcc aactttgttt cttactatct tatcagaaag ctgatagaga agctctacac
     6181 tgacaaagat aacaaaaccg aaaattccca tgaggaaagg accgaaaaac tccttgctga
     6241 tatattttat aagccgtttc attcttcttc ctccactgaa aaaaagagat ccagagagtt
     6301 ttttctgcat ctgtatttgt ttcctgttat agcaaatact atattctcag ctatatttgt
     6361 gctcatatcc gaaaaatgtt ctatgtttga aagaatatcc atatgaaggc ttatggactc
     6421 aatgctttct tttgatttat agaggtactt tttgtatgcg tgaaaaagtg aagaagcttc
     6481 gttattgagt gtgcatatat tctggcaggg attaaaaaaa acagaagaaa actcttcgtt
     6541 tttaagagtt ataaggttaa tatttatgaa atccgaaaaa accctcagag aagaagaaaa
     6601 agcttcctgg gtctgtttga aaaggtcttc gaagcttaag acatttattt tcggcggata
     6661 cttcatcaat tcaatgttaa gcttagcatt gctttcacac aggtcggcca tattctcaaa
     6721 aatataaacc agtttaagtc caagtaagtt ggccttaaga tatattcctg aaaaattagc
     6781 tttagaaaga aggaccattc cttcataatc aagcttggcc tcaagataat caacacggtc
     6841 atcctgagag atgatttcct ccgaaagagg aatatctttt tgaaagtatg catcactgaa
     6901 cttataaaac atgccctgga ctattctccc cattcttagt acgttgttca ggtatacaat
     6961 aagtggttct ctgagatatg gagtaagagg attttcctga atatattttt tttctttcat
     7021 tcaaatctcc tgccaaaagc taatttttgg ttcaaaagtt ctaaaaagct atacttttct
     7081 ttttgtgcga gaaataaagt tttcgaactt acccggacat ggactgattc tccttttttt
     7141 aaagaagaaa caatattacc gtcaacaagt acaaacccct tttcatttaa agcgttgttc
     7201 atacgtatgg ataagttttt ttcaggccca aagataagtg gtctcatatt aagaccgtgt
     7261 gccgcaagcg gaataagctc cattgctctg attgaaggat caagaatggc tccgccggca
     7321 gaaagaccgt aaccagttga accggtaggt gttgaaataa gtattccatc tccagaaaaa
     7381 gagtaaacag gttcgtccat ataactgaca gatatatcta tagtaccgtc cggagtgctc
     7441 cgctggacat tgagatcgtt caggcagaag tattttgttc cgttaaaatc agcttcaaga
     7501 agcattcttt cactgaaaga aagctttttc atagagatgc attctgccgc tgttcttatt
     7561 tcattctgtg aaaaagcagt cagaaaacca agctttccca tatttattcc gataatcggt
     7621 ttattaaagg ctactgccag aggaactgtc cgcagtacag tgccgtcacc gccaagaact
     7681 atgaaataat cattatcttg agcctgttct acaaagcagt ccttgcatgc ctgcacagca
     7741 tcagttatct ctatagaaaa gttttcaaac tcatttttaa tgttgtcatg gatataccta
     7801 acatcagttt tggtaggatt ataaaatata attactttca aaacgttcac ctcataataa
     7861 cagacagatc aaaaagagac gcaatgagga atttatcaac gaagataagc aacagcaatg
     7921 caagataagg cgtgaagtct ataggaccaa aagtgagagg tatcacacgt cttataggtc
     7981 tacatataaa atttgaaatc gagtcaaaaa aagaacgtac agggttatag taaggaagta
     8041 taaaactcag aacagctgaa attatgatag aaatctgaag aaagttaatt cctatccgaa
     8101 gcacaaaagc ggttccataa aagaagttgg caataaaaaa cattctcttt cctccgtttc
     8161 aggtatagat tcttgaacca aatatttttg aaccaattct aagcattgta gaaccatttt
     8221 ctattgctaa attgtaatca cctgacattc ccatagaaag ttctttaata tctggatact
     8281 ttaaagaaag gttatcgcgc agtttacgta aagaagaaaa aaccgaactt atgcattcgg
     8341 tatcacttgt gtttggagcc atggtcatca atccgcatac ttttatattc ggatacttta
     8401 gtgcattttc gataaaatcc tgtgcttggc ttagagatat acctgatttg gactcttcac
     8461 ccgaaacatt gacttctatc agaatattct gtacttttcc cagagaatat gctcttttat
     8521 cgatttcaat gacttcctga actctgcata cagagtgaat catgtgagag catgggacaa
     8581 tatactttaa tttattggtt tgaatactgc caataaaatg ccacataaga ccttcaagat
     8641 ttaactcgga ggatttttca cgaagttcct gagcccagtt ctctccgaaa tctctctggc
     8701 ctttttcaaa acattcaagt atatctgctg aggggaaagt tttggaaact gcaatgagtt
     8761 ttatttcatt ggaatttcgg gaatattttt ccgaaagctc agcgatttca ttcttcaaat
     8821 tcaggtaatt tttgattaac tgactgtcca atctgatcac cccgcatact gtttatttta
     8881 ctttcagagt atcccagata tcgtaaacct ttttctgtga tcatcctacc tctgctggtt
     8941 cttataagat agccgttttg aagaagataa ggctcataaa cctcacttat cgaatcaggc
     9001 tctattccaa gtgaagcagc caatgcttta actcccaccg gaccaccttt ataattctgt
     9061 gtcacggtat taagaagctt tctaaccact taattatcta aatatggacg tatatcaagc
     9121 atattcatta tttcttgaac ttcagagact tcaattctgc ttctttcctt tacctgggct
     9181 gtatctcgga ctctcttgag tagcctgttg gctatcctag gagtacccct tgatctttgg
     9241 gcaataagca gagcggcctc ctcggatatg gcggctttta gaagctctgc acttctacat
     9301 acaataagtt ttaaatcatc gtccgtataa aagctcattt cgagaatgat gccaaacct
//
LOCUS       DAOSHR010000073         9300 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_6315_length_9300_cov_5.617090, whole genome shotgun sequence.
ACCESSION   DAOSHR010000073 DAOSHR010000000
VERSION     DAOSHR010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 9300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9300
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6315_length_9300_cov_5.617090"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="PLU28_05400"
     CDS             complement(<1..443)
                     /locus_tag="PLU28_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpX C4-type zinc finger protein"
                     /protein_id="HOG34535.1"
                     /translation="MKEPKEKYCSFCGRNSNEAGTLIAGPNSLYICDDCVGLFHDLIE
                     DNKKNNVLSKNSEQVNLATPRQIKQELDKYVMGQESTKKAISVAVYNHYKRIKGITDK
                     SLLSDTEIEKSNVLMIGPTGTGKTLIARTLAKFLNVPFAIADATPL"
     gene            complement(535..1395)
                     /locus_tag="PLU28_05405"
     CDS             complement(535..1395)
                     /locus_tag="PLU28_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOG34536.1"
                     /translation="MKKSIFLMWLTVIFWGVSFVATKVVVDAIDPVIAAFIRFNIAWV
                     VMFVFIRKFNKYSKKDLFYLFVASFFGVTAYFLFENSGLMYTTPTNASLIISATPLVY
                     LLVSDILKKKLSGRIRYVGTAVAFGGVAILVMNGSFFLKLNPLGDLLMFGAVVSWVIY
                     TIAEEKLHHLDNKIVSRDINFLGALFFIPFLFFSEHKLTVWGKGEIGFQTVAGLIYLG
                     VFCSALGYIFWNKAIRDCGAKTATNGIYFIPVVTVVADAVILGNIPNIYALGGAALVL
                     AGTYISEIKS"
     gene            complement(1408..2049)
                     /locus_tag="PLU28_05410"
     CDS             complement(1408..2049)
                     /locus_tag="PLU28_05410"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOG34537.1"
                     /translation="MKIAWIGHSCFRMLSNGMTFVTDPFDETFGYKMPDIIADVVTES
                     HQHHDHNAHSRIKGKFVLINDDGEYRVDGCSIRGIKTYHDESLGIQRGNNTVYIFSFD
                     EGIRVVHMGDIGHAPDEKFFSEMGKVDILMIPTGGIYTVGPEKALRIVNRIEPKIVIP
                     MHYKTVHVGFALSQVDEFLASSRMTGIKMKELDTEKFADIKKPVCIIFEPFGD"
     gene            complement(2063..3658)
                     /locus_tag="PLU28_05415"
     CDS             complement(2063..3658)
                     /locus_tag="PLU28_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOG34538.1"
                     /translation="MESIRKLWQQLYDENGENELSLLMTVLEKYKNIESNSIKESLWY
                     KNSVVYSVYIERFGGTFKAAQEKLSYLKELGVTALWILPVLSSPMKDQGFDISDYYNV
                     RGNLGTNEDFFDFIHQAHMNGIRVVFDIAINHTSSDHEWFIDARKSKESKYRDYYIWN
                     DTPDKYARAGIVFDGIMKSNWEYNDQTSDYYFHRFYDSQPDLNYSNPRVMLEMINILL
                     FWQSKDIDGFRMDAAPYLYKQEGTDCYGLEKSHYILKIFRACLDYVSEGTVLLAEANE
                     KPIKVVKYFGNDDECHCAYNFPLMTGIFSAIAKADPKEISELFNPLVMPEIPSSCQWF
                     NFLRCHDELSLEAVGEEEKKNLVRSYLLDERWNFRKGAGISARTYDLFYNDYKKTVLA
                     FSVLFSLDGNPVIYYGDEIGMKNDYEVYEKIKKETGYDDTRYLMRSNFDVVLAQKIIE
                     GQDCNEKRIYDFIKYAANLRRCYRDLFLTAPEISSVMSVLKVKRRNLSKQLIVYNNLA
                     AEYAEVDGMKLEPYEARWIISYC"
     gene            complement(3724..4797)
                     /locus_tag="PLU28_05420"
     CDS             complement(3724..4797)
                     /locus_tag="PLU28_05420"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HOG34539.1"
                     /translation="MKAIVLCAGKGTRLRPLTFTNAKPLIPVANKPTIMYSLEMIKNA
                     GIVDVGIIVNPDNKKDFEAALGDGSRIGLNLSYHVQAEQKGLAHAASIAQEFIGEDTF
                     LMYLGDNMITVDLGKFIKDFEEKNLDSSILLTPVEDPSRFGIAVMEEDKVVRVIEKPK
                     EPPSNLAIVGVYIFKPMVFEGIKDLKPSARGEYEITDAIQWMISSGKTVGAKIIKEWW
                     KDTGKPEDLIEANRVILETFKTSFIEGNVYENSVVNGNVHIGKNSKIMGSIVRGPVII
                     GENCTITNAFIGPYTSVGNGANIEESEIENSIILDNARISNLKSRIDSSIIGAYAKIT
                     SIERKPNSIRFVIGDYGNVEIPK"
     gene            complement(4794..5552)
                     /locus_tag="PLU28_05425"
     CDS             complement(4794..5552)
                     /locus_tag="PLU28_05425"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HOG34540.1"
                     /translation="MFSELKNIVYECGKELLKWKKTGYDIRDKNGPDDIVTELDLRTQ
                     DLLMEKLLKKFGDIEVLAEEKDLDRRPQVSEYWVIDPIDGTVNFSRGMAEYCISVAYV
                     RGDEPEIGIVYVPQMDMMFACHRGYGAFLNDEKIMPCWSKNLSKSIITMGNIKGTTYK
                     YFELLEHQVMRIRIMGSAAIQTAYTASGFADAFISLKANAWDIAAGYLLLKESGGEVV
                     RFDGSKAGIYDQKALYANPYITGDLIKILKGWEK"
     gene            complement(5571..6326)
                     /locus_tag="PLU28_05430"
     CDS             complement(5571..6326)
                     /locus_tag="PLU28_05430"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOG34541.1"
                     /translation="MEKLQAIVQKAGLARRKAISLIQSGGVKVNGKVIIEPWHEVSAE
                     DKVEFGGYIYKVSDIIKADRKVYYLYNKPVDVLSSKSDTYGRKTITDDIKEFVKEDVF
                     NVGRLDYGTSGLIFLTNDGELADLLMHPRYKIEKTYQALIKGNPTGQELSMLENGVEI
                     LIEEKPYKTAPAKIEKVLKKGENSEVTVTITEGKKRQVRLMFRAINHNVIKLRRVKFG
                     PWSIEDVPNPGDIKKLNWKEVQKIKEDLKDRIK"
     gene            complement(6277..6816)
                     /locus_tag="PLU28_05435"
     CDS             complement(6277..6816)
                     /locus_tag="PLU28_05435"
                     /inference="COORDINATES: protein motif:HMM:NF016005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HOG34542.1"
                     /translation="MNEIDISTVEAVVFIKSQGISVKDISKILDMTEEKVLGILLLLQ
                     KKYSEAEHGVELAKNEDRFIFQIKPEIKRLVTPKPRKLELTASQFEVLAILYLNGPSR
                     LSEVEKSRGRNSYTQLLRLKELGLVKKTKKANTKSLFLYALSDNFFEWLSQDTIKKLE
                     EIKDGKITGNSTESGIGKT"
     gene            complement(6813..7487)
                     /locus_tag="PLU28_05440"
     CDS             complement(6813..7487)
                     /locus_tag="PLU28_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34543.1"
                     /translation="MGFYDIEIELDIFSGPFEKLVELISEKKLPVKKLPVSKIADIFI
                     SYVQNNKENLSDIGEFMRLAFYLSYLKSRELLPRSESDQELKRHRNTLYKIIEDYNLI
                     KSATQLIKDDFGTPNVRSVAVRNSNIVLSGDVTQQIDRFFKDFLLLQEKLQIVKELYT
                     VEMAVAMLKDYESFSIKDLFKLSENQKLKFVVFFLAVLNMLKDGAIEYGDGEFIRICD
                     KETVSI"
     gene            complement(7492..8472)
                     /gene="trpS"
                     /locus_tag="PLU28_05445"
     CDS             complement(7492..8472)
                     /gene="trpS"
                     /locus_tag="PLU28_05445"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOG34544.1"
                     /translation="MKIVSGMRPTGKLHLGHLMPIEKWVELQNSGNDTQFFIANWHAY
                     TSHRDTPQIIDDATLDLVRHYLACGINPEKSIVFIQSAVKEHAELSLLLGMLISVSRL
                     ERVPTYKEMQENVKDKDLSTIGFLGYPVLMATDILIYKADAVPVGEDQIYHLELAREI
                     ARKFNMTYGVEILKEPQPIISRVAKLPGTDGRKMSKSYDNSILIDSNAQELKSKIMPM
                     ITDPARMKRTDPGDPEKCPVWEFHKAFTNSESEKQWVTGGCKSAGIGCIDCKKVLLKN
                     LEAKMQPIWDNLSKISEDDARGVVEKGNARAREIACNTMSEVRCAMKLRW"
     gene            8586..9245
                     /locus_tag="PLU28_05450"
     CDS             8586..9245
                     /locus_tag="PLU28_05450"
                     /inference="COORDINATES: protein motif:HMM:NF027563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4897 domain-containing protein"
                     /protein_id="HOG34545.1"
                     /translation="MFWKKDKNSEPVNPELAREVNRKKSNSNFMIITIIFFAGLFVVN
                     YFMFQGMQVKYDVISQKICYIVNEDMSVILSSDMNLRANDQKSYDKLIEGFKTSDSEK
                     KKKYEEFLDGLEKDSQKQLQVISYSSTSTTQDLYLTVKEDVRITGFIEKSDDYTYIFN
                     LKNQKLKIDDKTSLYIYAPEGWIIQSAVPVPLIIENEYAMWKNAGQMDFPNVIMIRQP
                     Q"
BASE COUNT         2683 a         2031 c         1502 g         3084 t
ORIGIN      
        1 agcggagttg catcggctat agcaaaagga acattcaaaa attttgccaa ggttcttgca
       61 ataagagtct tgcctgtacc tgtgggacca atcatcagaa catttgattt ctcgatctct
      121 gtgtcagaaa gaagactctt gtctgtaata cccttaatac gcttatagtg attgtatact
      181 gcaaccgata tggctttttt agtgctttcc tgtcccatta catatttgtc aagctcctgc
      241 tttatctgcc ttggagtagc caaatttacc tgttctgagt tcttggaaag gacattgttt
      301 ttcttattgt cttctataag gtcatgaaaa agtcccacac agtcatcaca tatatacaga
      361 ctgttcggtc ctgctattaa tgttcctgct tcattggagt tgcgcccgca aaacgagcag
      421 tacttttctt ttggttcttt catcagttta attcctccta agtttcattc taataattca
      481 atatagatta tatcgttatt tttttaaagc catggcggtt ttgtaatttt gacgttaact
      541 tttgatttct gaaatgtaag ttccagccag caccagagct gctccgccta aggcatatat
      601 atttgggata ttcccaagta taacagcatc agcgacaact gtaactacag gaataaaata
      661 aataccgttg gtggctgtct ttgctccaca atcgcgtatg gccttattcc aaaaaatata
      721 tcccagtgct gaacagaaaa ccccaaggta tataagtccg gccacagtct gaaaacctat
      781 ctctcctttt ccccatacgg taagtttgtg ttctgagaaa aaaagaaaag gtataaaaaa
      841 taatgcacca agaaaattaa tgtctctaga aactatctta ttatcaagat gatgaagttt
      901 ttcttcagca atagtataga tgacccatga aacaacagcg ccaaacataa gaagatctcc
      961 cagaggatta agcttgagaa aaaaacttcc gttcatgacg agaatggcta ccccgccaaa
     1021 agcaaccgca gttcctacat aacgtatcct tcctgaaagc ttttttttca gtatatctga
     1081 aactaaaagg tataccagag gagttgcaga tattataagt gaggcatttg tcggcgtagt
     1141 atacataagt ccggaatttt caaataaaaa atatgccgtt actccaaaaa aagatgccac
     1201 aaaaagataa aaaagatctt tttttgagta tttattgaac tttcttataa aaacaaacat
     1261 gacaacccat gcaatgttaa atcttataaa agccgcaatt acaggatcta ttgcatctac
     1321 aactaccttt gtggcaacaa acgagacacc ccaaaaaata accgtaagcc acatcaaaaa
     1381 aatacttttt ttcaacttac tcctccatca atctccgaat ggttcaaata taatacatac
     1441 gggctttttt atatccgcaa acttttctgt gtctaactcc ttcattttta tgcctgtcat
     1501 tcttgaactt gctaaaaact catctacctg tgaaagggca aatccaacat ggacggtctt
     1561 atagtgcatg ggaataacaa tttttggctc tattctgttt actattcgta atgctttttc
     1621 aggaccgacc gtatatatcc ctcctgtagg aatcataagt atatcaacct ttcccatttc
     1681 actgaaaaat ttttcgtctg gagcatgccc tatatccccc atatgaacaa cccttatgcc
     1741 ctcgtcaaaa gaaaagatat agactgtatt gtttcccctt tgaataccta aagattcatc
     1801 atgataggtc tttatgcctc ttatagaaca gccgtccact ctgtactcgc catcgtcatt
     1861 gataagaaca aacttgcctt taatccttga atgggcatta tgatcatgat gttgatggct
     1921 ttccgtgaca acatctgcaa ttatatcggg cattttatat ccgaatgttt catcaaaagg
     1981 atcggtcacg aacgtcatac cgttggaaag cattctgaaa caagaatgtc caatccacgc
     2041 tattttcatt ttttccgcct ccttagcaat aagaaattat ccatcttgct tcgtatggtt
     2101 caagcttcat accatcaacc tcagcatact ctgccgcaag attattgtaa acgatcagtt
     2161 gctttgaaag atttcttctt tttaccttaa gaacagacat tacggaagaa atctccggtg
     2221 cagttaaaaa cagatctctg tagcatcgtc tcagattggc ggcatacttt atgaagtcat
     2281 agattctttt ttcgttgcag tcttgccctt ctattatctt ttgggcaaga accacgtcga
     2341 aattacttct cataaggtat cttgtatcgt catatcctgt ttcctttttt attttttcat
     2401 aaacctcata atcatttttc atacctatct catcaccgta ataaatcacc ggatttccat
     2461 ctaatgagaa aagcaccgaa aacgccagca ccgttttttt gtagtcatta tagaagagat
     2521 cataggttct tgccgatatc cctgcacctt ttctgaaatt ccacctttca tctaaaagat
     2581 agctccgtac gagattcttt ttctcttctt cacctactgc ttccaatgaa agctcatcat
     2641 gacaccttaa aaagttaaac cactggcatg aggaaggaat ttcaggcatc actaagggat
     2701 tgaaaagctc tgaaatttct ttgggatctg cttttgcgat tgctgaaaaa atcccagtca
     2761 taagcggaaa gttatatgcg cagtgacatt cgtcatcatt cccaaaatat ttcacaacct
     2821 tgataggctt ttcattagcc tcagcaagaa gaacagttcc ttcagaaaca tagtccagac
     2881 atgccctgaa gatctttaag atataatgtg atttttcaag cccataacag tctgttccct
     2941 cctgcttgta aagatatggc gcagcatcca ttctgaaccc atcaatatct tttgattgcc
     3001 aaaaaagcag gatattaatc atctcaagca tcactcttgg atttgaatag ttaagatctg
     3061 gctgactgtc gtaaaatctg tggaaataat agtctgatgt ttgatcgtta tactcccagt
     3121 tacttttcat tataccgtca aatactattc ctgctcttgc atatttatcc ggagtatcgt
     3181 tccatatgta gtagtccctg tactttgatt cctttgattt tcttgcatcg ataaaccatt
     3241 catgatcgct gcttgtatgg ttgatagcta tatcaaaaac aactcttatt ccattcatgt
     3301 gggcctgatg tataaaatca aaaaaatcct catttgttcc aagattacct ctcacattgt
     3361 aataatcact aatatcaaag ccctgatcct tcataggaga gctcagaacg gggagtatcc
     3421 acaatgcagt tacgccaagc tcctttaaat aagaaagttt ctcttgggca gctttgaagg
     3481 ttccgccgaa tctttcgata taaaccgagt ataccacgga attcttatac cataaacttt
     3541 cctttatgga gttactttcg atatttttgt acttttcaag gactgtcatt agcagtgaaa
     3601 gttcattttc gccattttca tcataaagct gctgccaaag ttttcgtata ctttccatct
     3661 ggtcctccta aaaaaattcc acagacagtc acctgtccat ggagtataaa ggatgtaagt
     3721 aatttatttg ggtatctcca catttccgta atctccgatc acaaatctta tggagttggg
     3781 cttacgttct atgctcgtta ttttggcgta tgcacctatt atggaagagt ctattcttga
     3841 cttaagattg cttattcgtg cattatccaa tattattgaa ttttctatct ccgattcctc
     3901 tatattggca ccattaccta ccgatgtgta tggacctata aaagcattgg taatagtaca
     3961 gttttctcct attatgacag gccctcttac tatcgaaccc ataatctttg aattcttgcc
     4021 tatatgaaca ttgccgttca ccacactgtt ctcataaaca tttccttcaa tgaaacttgt
     4081 tttaaaggtt tcaagaataa ctctattggc ctctataaga tcttcgggtt tgccggtatc
     4141 tttccaccat tcttttatta tcttggcacc gactgtttta ccggaagata tcatccactg
     4201 tattgcatcg gtaatctcat actctcctct ggctgaaggc tttaagtctt ttatcccctc
     4261 aaaaaccata ggtttgaata tgtatacacc tactatagca agattcgaag gcggctcttt
     4321 aggtttttca attactctga ctactttgtc ttcttccatt acagctatac caaaccttga
     4381 aggatcctca acgggagtaa gaagaattga agagtcgaga tttttttcct caaaatcctt
     4441 tataaacttt ccaagatcga cggttatcat gttgtcgccc agatacatca gaaaagtgtc
     4501 ttctcctata aattcctggg ctattgaagc agcatgagcc aatccttttt gttcagcctg
     4561 cacatgatag ctaaggttga gacctattct gctaccatct cccaatgcgg cctcaaaatc
     4621 ttttttgtta tcaggattga ctattatgcc gacgtctact attccagcat tttttatcat
     4681 ttcaaggctg tacattatgg ttggtttatt agctaccgga ataagcggct ttgcattagt
     4741 aaatgtcaat ggtcttagtc ttgttccttt tccagcacaa agtactatag ctttcatttt
     4801 tcccacccct ttagtatttt tatcagatct ccggttatgt atggattagc atataatgct
     4861 ttttgatcgt atattccagc tttgctgccg tcaaagcgga caacctctcc accgctttct
     4921 ttaagtagaa gatatccggc agctatatcc catgcatttg ccttaaggct tataaacgca
     4981 tcggcaaaac ctgaagcggt atacgctgtc tgtattgctg cactgcccat tatccttata
     5041 cgcataacct gatgctccaa aagttcaaag tacttgtagg tagtaccttt tatgttaccc
     5101 attgttatta tcgacttgct cagatttttc gaccagcagg gcataatctt ttcatcatta
     5161 agaaaagctc catatcctct gtgacaggca aacatcatat ccatctgcgg aacatatacg
     5221 atgcctattt caggctcatc gccacgcaca taagctacgg aaatacagta ctctgccatg
     5281 cctcttgaaa agtttactgt tccatctatt ggatctatca cccaatactc actgacctgc
     5341 ggtcttctgt ccagatcttt ttcctcagca agaacctcta tatctccaaa tttctttaga
     5401 agcttttcca taagaagatc ctgagttctc aaatcaagct ctgtgactat atcgtccggt
     5461 ccatttttgt ctcttatatc ataacccgtc ttcttccatt tcaacagctc tttaccacat
     5521 tcatacacta tatttttcag ctctgaaaac aaatttttca ccccgttata ctacttgatt
     5581 ctatctttca agtcctcttt tattttttga acctctttcc agttaagttt ttttatgtcc
     5641 ccaggattag gcacatcctc aatcgaccac ggaccgaact tcactcttct gagttttatc
     5701 acattatgat tgattgctct gaacatgagc cttacctgac gctttttccc ctctgttatg
     5761 gtaactgtta cctctgagtt ttcacccttt ttaagaactt tttcaatctt ggctggcgca
     5821 gttttgtatg gtttttcttc aatcaatatt tcaacaccat tttccagcat cgaaagttcc
     5881 tgaccggtag gattaccctt gataagcgcc tgataagttt tttcgatttt atatctagga
     5941 tgcataagaa gatcggcaag ctccccgtcg tttgtaagga atatcagacc agaagtaccg
     6001 taatcaagtc ttcctacatt gaatacatct tcctttacaa actcttttat atcgtctgtt
     6061 atggtttttc ttccatatgt atcgcttttt gaagaaagaa catcgacagg tttattgtac
     6121 agataataaa cctttctatc tgcctttatt atgtcactaa ccttatatat gtacccgccg
     6181 aactcaactt tgtcttcagc agagacttca tgccaaggct caataatcac tttgccgttt
     6241 acttttactc ctccgctctg tatcagggaa attgctttac gtcttgccaa tcccgctttc
     6301 tgtactattg cctgtaattt ttccatcttt tatctcctca agttttttaa tcgtatcctg
     6361 tgaaagccat tcaaaaaaat tatcgctaag agcataaaga aaaagcgatt tagtgttggc
     6421 tttctttgtc ttttttacca gcccaagttc tttgagcctt aaaagttgtg tgtagctgtt
     6481 ccttcctctt gacttctcca cctcagaaag tcttgaggga ccgttaagat aaagaatagc
     6541 caaaacctca aactgcgatg ctgtcagctc aagcttacgt ggtttgggag tcacaagcct
     6601 ttttatctcc ggttttatct gaaagatgaa cctatcttca ttcttagcca gttcgacacc
     6661 gtgttcagct tcagagtact ttttctgtaa cagcaacaaa attcccagaa ctttttcttc
     6721 cgtcatatcc agaatttttg atatatcttt tacgcttata ccctgggatt ttataaaaac
     6781 aacagcttct acagtggaaa tatctatctc attcatatgc tgaccgtctc tttatcacat
     6841 atacgtataa actcaccgtc tccatactca attgcaccgt ctttgagcat gtttaaaacc
     6901 gccagaaaaa aaaccacaaa cttaagcttt tggttttcag acagtttaaa aaggtcttta
     6961 atactgaacg attcgtaatc tttaagcata gcgactgcca tctcaacagt ataaagctct
     7021 ttcacaatct gaagcttttc ctgcagaagc agaaaatctt taaaaaatct gtctatctgc
     7081 tgtgtgacat ctccggacag aacgatgtta ctgtttctta cagcaacact tctgacatta
     7141 ggcgttccaa aatcatcctt tataagctga gttgcagact ttataagatt atagtcttct
     7201 ataatcttat aaagggtatt acgatgcctc ttaagttcct gatcactttc agaacgcgga
     7261 agaagctccc ttgactttag atatgaaaga taaaatgcga gtctcataaa ctcgcctata
     7321 tcagaaagat tttccttgtt gttttgaaca taacttataa aaatatcggc aattttcgaa
     7381 accggcagtt ttttcaccgg tagcttcttc tcacttatga gttctaccag tttttcaaac
     7441 ggaccggaaa atatatcgag ctcaatttct atatcgtaaa atcccatccg gttaccacct
     7501 tagtttcatg gcgcatcgaa cttcactcat agtattgcat gcaatctccc ttgccctggc
     7561 attacctttt tcaactaccc ctctggcatc atcttcagat atctttgaaa ggttatccca
     7621 tatgggctgc attttggctt caagattttt cagaagaacc tttttacagt caatacaacc
     7681 tatacccgcg cttttacatc ctcccgtaac ccactgcttt tcactttcac tgttagtaaa
     7741 agctttatga aactcccata ctggacattt ttcagggtca ccgggatccg tacgcttcat
     7801 ccttgccgga tcggttatca taggcattat cttggacttt agctcctggg cattgctgtc
     7861 aataagaata gagttatcat aagacttact catctttctt ccgtctgttc ccggaagttt
     7921 tgcaactctg gatattatag gctgaggttc tttaagtatt tcgactccat aggtcatatt
     7981 gaactttctg gctatttccc tggcaagctc aagatggtat atctggtctt cgccaactgg
     8041 aacagcatca gctttatata ttagaatatc tgttgccata agtacaggat agcctaagaa
     8101 tccaatcgta gaaagatcct tatctttaac gttttcctgc atctctttat atgtcggcac
     8161 tctctcaagc ctggaaaccg atatgagcat acccaaaaga agagaaagct cagcatgctc
     8221 tttaacggca gactggataa acactatcga tttttcagga tttataccac aggcaagata
     8281 atgccttaca agatcaagcg tagcatcgtc aataatctga ggggtatctc tgtgggatgt
     8341 atatgcatgc caattggcta taaaaaattg tgtatcgtta ccagaatttt gaagttctac
     8401 ccatttttct ataggcataa gatgtccaag atgcaacttg cctgtcggcc tcattccgct
     8461 tacaatcttc atgttacacc tccaaaaata tgaattacaa gaatatttta tcatacaatt
     8521 ggtatatttt catgatttta tagtaaaata taaaaagaat cagattttac cagggaggta
     8581 tttaaatgtt ttggaaaaaa gataaaaaca gtgaacctgt gaaccctgag cttgccaggg
     8641 aagttaacag gaaaaagagc aattcgaact ttatgataat aactataata ttttttgcag
     8701 gcctttttgt tgtaaattac tttatgtttc agggcatgca ggttaaatat gatgtaatat
     8761 cgcaaaaaat atgctatatc gtcaacgaag atatgtccgt aatactttca tcagatatga
     8821 atttaagagc caatgaccag aaatcctacg ataagcttat tgaaggtttt aaaacttctg
     8881 attctgaaaa aaagaaaaaa tacgaagagt ttcttgacgg tctggaaaaa gatagtcaga
     8941 agcagctgca ggttatctca tactcctcta cttcaactac tcaggacctt taccttaccg
     9001 tgaaagaaga cgtgagaata actggattta ttgaaaaatc cgatgactat acttatatat
     9061 tcaatttaaa aaaccagaag ttaaagatag acgataaaac atccctatac atttatgcac
     9121 cggaagggtg gataatacag agtgccgttc ctgttccgct gataatagaa aacgagtatg
     9181 caatgtggaa aaatgctggt cagatggatt ttccgaatgt aataatgatc cgccagcctc
     9241 agtaaggtgc tgaccattaa tgtctcttta tattatatgg ttcttgctga tcttaagtct
//
LOCUS       DAOSHR010000074         9128 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_6466_length_9128_cov_5.434035, whole genome shotgun sequence.
ACCESSION   DAOSHR010000074 DAOSHR010000000
VERSION     DAOSHR010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 9128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9128
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6466_length_9128_cov_5.434035"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="PLU28_05455"
     CDS             <1..531
                     /locus_tag="PLU28_05455"
                     /inference="COORDINATES: protein motif:HMM:NF021596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HOG34546.1"
                     /translation="YQFTHAWIDFRKLYDNNGVNWYENSLKATKAARQYCIDLKGKYK
                     TFGECSWGLTACDSPDGYRGDFGAAPNGENSTVGKTDGTIPPAGAIGSIVFVPDEVID
                     AMNHYRTFDNLWGRYGFKDAYNLDKNWFSKTHIGIDKGISLLMIENYLSGLIWEVTMR
                     NKYIKKGLEKLLIIPK"
     gene            complement(587..1117)
                     /locus_tag="PLU28_05460"
     CDS             complement(587..1117)
                     /locus_tag="PLU28_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34547.1"
                     /translation="MKKLLLVLSLVSILMVSVFATDMNLADINWTINSDLKISRTVSV
                     KVYQWIYASVSSNKTIEITKPGSESFDLFNIAVGSNKGATLRVEMTGLPDQLKLEEKY
                     VKYDVTEPATPIGEAGVFLKDDLVLPGYNADTGTVTFKDMVFGAKFNASPILIANKDN
                     PYTITVNFSFEPTVRF"
     gene            complement(1254..3656)
                     /locus_tag="PLU28_05465"
     CDS             complement(1254..3656)
                     /locus_tag="PLU28_05465"
                     /inference="COORDINATES: protein motif:HMM:NF024298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HOG34548.1"
                     /translation="MSKRSIAVIVILVFHIISLFAADIIVSAYSSLPIKGLYISPKSF
                     VVPLSSENLIRLLNKDGTIRSVYETSAEPNAVVFSEDESVIACANVNGSVDIFNLTNP
                     IANFKLTESKESITSIDMKDQKIIFTSYDKKVFFYNISKRKKQFEFPIGSIPVCSKFI
                     SKELAIVGDFNGNAYMFSENGIKYTNKIFSSAIRNIFIYQDRLIFVGSEGKVVVTDLS
                     MNIKSVLPLENNIQSAILSPDSNYLGILYNNNSFKVYSIEGILGSTLKVILNITALPF
                     RCAALAWTNSWREILLTEGSDMYRITFPSGILEKTVENMVSLPKKLFWKDNYLVFADK
                     ISNFGIIDIESGKLISFFNTQTAGINDFIVIDDKYIVTVGTDGIARSYDLNTGKKAKE
                     IFLSDSQLTSVDISPDKKFIVFGGWDNKVYVYNYPSFTKYRVVSDLHDNWINDIAINR
                     NGSYIAVSSYDRKVSISKFPDFESSSRKFFSFKYNIWSVSWAHAKEFLATGGYDGILE
                     IWDALYQMSIKPGISTPSIRDIEWNIDDTKIVTACEDGNIYLWNAKSGELISTVNVSS
                     GPVLDIYWNKGSKYIACIADKNVLKILDIEEKKCIATIMIFKDGKYVSFKENGEYMTN
                     IPSQSESIYFSRYNPLMLIDTLMLKKVDNLNLREVEPPVIACPDNFILSPQNNTINIE
                     ISDNRMLKQVKIGTRLYDVNQKDFSVDFKIDIEKLIADEIQILAYDDSGNKSIKTVPL
                     EYQNISLIAIANEVVREESGKYIGVLSKGMTVKLLGIFNESYIIDFMGKKGVIAKYFL
                     IK"
     gene            3813..4928
                     /gene="dnaN"
                     /locus_tag="PLU28_05470"
     CDS             3813..4928
                     /gene="dnaN"
                     /locus_tag="PLU28_05470"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOG34549.1"
                     /translation="MKFEINKKTLSSLVDMAEKAVAKKTTNPILSGIKIEVAAKELNV
                     YATDLQTGFHGKTTVEDSQEDCAFVVDHKNFSEILKSLPVVSIIINYDNGVLSLESAT
                     TAFKLPTMNAAEFPNVIPSVAGSTVDLEREKILHMIEKVIFCASRDSDPLSRNLNSVY
                     WDFRSGGFLSLVATDSYRLGISEINLQQSSLVSSFLLSLRSVEELRNVLLSCRSKTFK
                     LNFDGSRALFSFSEDSVELALNVIDSKFPNYMDILPKAFKTKMVLSTAEFSEILRRVS
                     IASGSSEQVKMDVNEELLTLSANSADVGEGKDTITVEKEGNNILIAYSPRYLREAVEK
                     IETTEFEFNISGETNPTVLKPFNDNNYMYIVMPIRLV"
     gene            4931..5431
                     /gene="ispF"
                     /locus_tag="PLU28_05475"
     CDS             4931..5431
                     /gene="ispF"
                     /locus_tag="PLU28_05475"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HOG34550.1"
                     /translation="MDLRTGLGYDIHPLVQGRPLVVGGVIIENSKKLGLEGHSDGDVL
                     THAFMDAIIGACGYGSIGEFFPQEPQWKDSRSIFLLKELCRVLSGKVTIEIFNFDCIV
                     ISDIVKITQYGQEMKENLSDAVGVLPQKINIKGKSGNGVGSSSSSKSVEAYVSVLLSI
                     DSSRRE"
     gene            5434..6582
                     /locus_tag="PLU28_05480"
     CDS             5434..6582
                     /locus_tag="PLU28_05480"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG34551.1"
                     /translation="MNQMKTRNYLYFLHFLTSIIINSVAPLMTTIQSEYTVSISQSAL
                     VPLLNTAGVLISNFAGGFLIASMGLKTSLQLGLLFGLLGTALFATSYSFLQVLMGVFF
                     IGMTTGTVFMSLTSIFASLDSKYANYGLFHAFFGIGGIVSPLITAFFLKANFSYRNIF
                     ALYTVVMALILVYTSMLKYLENTRHPAIQPKEALGIISRPLVYLTLLIFLLYAGTEIG
                     ITTWSGNLFEKAFLLSKSNAQLLLSTFWAAYTFGRIITEYIDRKLGAIRTIILSSVMV
                     IISIAAMLTIKNSVMFIFIGLFLGPIFPTVQKYANSKLSQREIGLYSGLSYGFTGFGS
                     MLIVTSMGLAADVDTRLSYVIALFTFTVIIFIAAALGRKKGGQENAKN"
     gene            6569..7045
                     /locus_tag="PLU28_05485"
     CDS             6569..7045
                     /locus_tag="PLU28_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34552.1"
                     /translation="MQKIRKVVYVMFVFVFLFILSGCSSQPKTTQNMLDTKGLVTTRD
                     FKDVKGFGVIDYTKFPNPSQAILAAHEAALVDAYAKATEFVYGLIVEGGSSVENFALK
                     DKTVETRLRGVIRGAEEISTEGSKDTDGDGIYAVVLRIYRKAIEETLGMKLQAWTY"
     gene            complement(7086..7754)
                     /locus_tag="PLU28_05490"
     CDS             complement(7086..7754)
                     /locus_tag="PLU28_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34553.1"
                     /translation="MKKKGFVALILSVLCMSLILTSCSSGVPEGMKPLKVGTTTIKYI
                     PKDALIDVYPMPYDEKMILELLQNNLFVEFEGTYSGGIVDKSMYYEMADLAARTELSK
                     FLDTIVQGYSAKVSGILSEVKTTQNNADVNEMVASAGKTVQEAFTVSRQQGAKVLCKY
                     YIPTGNKYEYHVSIFYNPQAVINYLEQTELIKKAGDQQVQLQQALNDVIKEAKKGTPM
                     DQKQ"
     gene            complement(7770..8405)
                     /locus_tag="PLU28_05495"
     CDS             complement(7770..8405)
                     /locus_tag="PLU28_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34554.1"
                     /translation="MQKTKVLIFLILSTFFLSSCSLQPKTDYKADKSQDAFKPLIIGN
                     VTVAMIPPNAVITDINYSISRRVSELSESGSFYVINSWAIGGRFLSDQHYKEAEDKAK
                     VQLSEFLSTQVKSIKSKTTYLNGKNEISVYSSLESALSESELKGSMVLGRYYIDTMSL
                     REYHVLVFYDPVTVLKIIKESKNAVSSLDTEGIDVDDLFTLINEEISKAKD"
     gene            complement(8510..8740)
                     /locus_tag="PLU28_05500"
     CDS             complement(8510..8740)
                     /locus_tag="PLU28_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34555.1"
                     /translation="MLNKSVEHLIMKRYIFVNYSRRSGYAINREKLDKFSLNYIAGLL
                     DSYPVLNEIPEFVTYLKKRAFAERVVSFGKKL"
BASE COUNT         2711 a         1661 c         1805 g         2951 t
ORIGIN      
        1 tatcagttta cacatgcatg gatagacttc cgcaagctat atgataacaa cggagttaac
       61 tggtacgaaa attctttgaa ggcaactaaa gccgcaagac agtactgtat agacctcaaa
      121 ggaaagtata aaacttttgg ggaatgctca tgggggctta ctgcctgcga ctctcccgat
      181 ggatacaggg gtgactttgg ggctgcgcct aacggtgaaa atagtactgt gggtaaaact
      241 gacggaacta ttcctccggc aggggctata ggctccatag tatttgttcc ggatgaggta
      301 attgacgcaa tgaaccatta cagaaccttt gataatcttt ggggaaggta tggctttaag
      361 gatgcttaca atttggataa aaactggttt tcaaaaactc atataggcat tgacaaagga
      421 ataagccttc tgatgataga aaattatctg tcgggtctca tatgggaagt taccatgagg
      481 aataagtata ttaaaaaagg attagagaag cttttgataa tacccaagta agttgaaaaa
      541 agttcccgca tcagcgggaa ctttttttat ttttataagg tattaattaa aacctgacag
      601 taggttcgaa agaaaagtta acagttatag tataagggtt gtctttgttg gcaatcagta
      661 taggtgaagc attgaatttt gctccaaaaa ccatatcctt aaatgtaacc gtacccgtat
      721 cggcattgta tcctggaaga actaaatcat cctttaaaaa tactccggct tctcctattg
      781 gagttgcagg ctctgtaacg tcgtacttta catacttttc ttcaagcttt agctgatctg
      841 gaagaccagt catttcgact ctcagggttg ctcccttgtt tgaacctaca gcaatgttga
      901 aaagatcaaa gctttcagaa ccaggttttg taatttcaat agtcttgttt gaagatacgc
      961 ttgcatagat ccactgataa actttaacac ttaccgttct gctgatctta aggtcagagt
     1021 ttattgtcca attaatatct gcaagattca tatcagttgc aaaaactgat accataagaa
     1081 ttgacactaa agataaaacc aataataatt ttttcatatc aaacacctcc tatagttttt
     1141 gttttcaaga taatactacc gcttaaatgt tacgagtcaa ggttgaatgt aaaatattag
     1201 ttaacaaaaa ttaaattgta attaaaagct gttttatatt ttttgttaaa taatcacttg
     1261 ataaggaaat actttgcgat aactcccttt tttcccatga agtctataat atagctttca
     1321 ttgaaaattc caagaagctt tactgtcatc cctttgctaa gaactcctat gtactttccg
     1381 ctttcttccc gcacgacttc gtttgctatg gctataagac ttatattctg atactcaagc
     1441 ggaacggttt ttatggactt attgccactg tcatcataag ccaggatttg tatttcatct
     1501 gctataagct tttctatatc tattttgaaa tctaccgaga aatccttttg gtttacatcg
     1561 taaagcctgg ttcctatctt tacctgtttc aacattctgt tatctgaaat ttctatattt
     1621 atagtattgt tttgcggtga aagaataaag ttatcaggac atgctataac tggtggttct
     1681 acttcacgca ggttcagatt gtcaactttt ttgagcatca gcgtgtctat aagcatcaga
     1741 ggattgtatc tggaaaagta tatgctttcg gactgtgatg gaatgttggt catatactcg
     1801 ccgttttctt taaacgatac atactttccg tctttaaaaa tcattatggt ggcaatacat
     1861 tttttttctt ctatatccaa aatcttaaga acgttcttgt cggctataca ggcaatatac
     1921 ttgcttccct tgttccagta tatgtccaga acaggaccgc tggatacgtt gactgtgctg
     1981 atcagttcgc cgctctttgc attccagaga tatatatttc catcctcaca ggcagtaact
     2041 atttttgtgt catcaatatt ccattctata tcacggatgc ttggagttga aatgccaggt
     2101 tttatggaca tttgatacaa agcatcccat atctcaagta ttccgtcgta tcctccggtg
     2161 gcaaggaact cttttgcatg agcccagctt acgctccata tattatactt aaaagaaaaa
     2221 aatttccggc tggagctttc gaaatcagga aattttgata tgcttacctt cctgtcataa
     2281 gaggatacgg cgatataact gccgttgcgg tttatggcta tatcgtttat ccagttgtca
     2341 tgaaggtctg aaactaccct gtactttgtg aaggagggat agttgtaaac gtatacttta
     2401 ttatcccagc ccccgaaaac aataaatttt ttatctgggc ttatatcaac cgaagtaagc
     2461 tggctgtcgg ataaaaaaat ttcctttgcc ttttttcctg tgttaagatc ataggatctt
     2521 gcaataccgt ccgtgcccac ggtgacaata tacttatcgt ctattacaat gaaatcgttt
     2581 attccggcgg tctgagtgtt gaaaaaagag ataagctttc cggattctat atcgattatt
     2641 ccaaaatttg aaattttatc tgcaaagaca aggtaattgt ctttccaaaa aagttttttg
     2701 ggaagggaga ccatattttc aacagtcttt tctagtattc ctgaaggaaa ggtaattcga
     2761 tacatatcag aaccttcggt aagcagtatt tcgcgccagc tgttagtcca agctagtgca
     2821 gcgcatctga aaggaagggc tgttatgtta agtataactt tcagggtact cccaagaatg
     2881 ccttctatgc tgtaaacctt gaaggagttg ttgttgtata gtataccgag gtaatttgaa
     2941 tccggggaga gtatagcgga ttgaatatta ttttcaagtg gaagcactga ttttatattc
     3001 attgaaaggt ctgtcacaac aactttgcct tcggaaccga caaatataag tctgtcctgg
     3061 tatataaaga tgtttcttat tgcagagctg aaaattttgt ttgtatattt tattccgttt
     3121 tcgctgaaca tataagcatt cccgttaaaa tcacctacta ttgccagttc ttttgatatg
     3181 aacttggagc atacgggaat tgaaccaata ggaaattcaa actgtttttt tcttttggag
     3241 atgttgtaaa agaatacttt tttatcatag gatgtaaata tgattttctg atctttcata
     3301 tcaatagaag ttatgctttc tttggactct gtgagcttga aattggctat aggattggtc
     3361 agattaaaaa tatcaacact tccgtttaca tttgcgcagg caataactga ttcatcttca
     3421 gaaaagacga ctgcattagg ttcggcagag gtctcataaa cagaacggat tgttccgtct
     3481 ttgttcagaa gacggattaa gttttcagaa gaaagtggta ccacaaaaga tttaggactt
     3541 atgtatagtc cttttatggg aagagaactg tatgcactta cgattatatc tgctgcaaat
     3601 aatgatataa tatggaaaac cagtatgacg attacagcta tgcttctttt actcattggg
     3661 gtatatctcc tgcagatctc tcattcttaa actattatat cataaaagaa agtgttttat
     3721 agacttttaa tttgcaaagt cagattgttg attttttgta tggttttgag gtaaaatcta
     3781 ttaaaatgaa attaactgag gaggtacaga atatgaagtt tgagattaat aaaaaaaccc
     3841 tttcgtctct ggtggatatg gcagaaaagg ctgttgcaaa aaagactacc aaccctatcc
     3901 tttcgggaat aaagatagag gttgccgcaa aagagctaaa tgtatatgcc acggatcttc
     3961 agacgggttt ccacggaaaa accacggtgg aggattctca ggaggactgc gcttttgtgg
     4021 ttgatcacaa aaatttttca gagattctca aatccttgcc tgttgtaagt ataatcatta
     4081 actacgataa cggagttctc agccttgaat cggcaactac tgcttttaag cttccaacaa
     4141 tgaatgctgc tgagtttcca aacgttattc cttcagtggc ggggtcgacg gtggaccttg
     4201 aaagggaaaa gattcttcat atgatcgaaa aagtaatttt ctgtgcatca agagattcgg
     4261 atccgctctc cagaaatttg aactcggttt actgggattt caggagcggg ggttttttaa
     4321 gtcttgttgc gacagacagc tacagactgg gcatttctga aataaatctt cagcagtcaa
     4381 gccttgtttc gtcgttcctt ctatccctga gaagtgttga agaactcagg aatgtactgc
     4441 tttcatgccg ttccaaaact ttcaagctta acttcgacgg ttcaagggct cttttctctt
     4501 tctccgaaga ttcagtagag ctggcactta atgttataga ctccaaattt ccaaattata
     4561 tggacatact gccaaaggct ttcaaaacca aaatggttct ttctacagca gagtttagcg
     4621 aaattctgcg aagggtttct atagcaagcg gttcttcaga acaggttaaa atggacgtta
     4681 acgaggaatt gcttacattg agcgccaatt ctgcagatgt tggggaagga aaggatacaa
     4741 taacggttga aaaggaagga aacaacatac tcatagcata ctctccaaga tatttgagag
     4801 aagcggttga aaaaatagaa acaaccgaat ttgagttcaa tatatccgga gaaaccaatc
     4861 ctacggtttt aaaacctttt aacgataaca actatatgta tatagtaatg cctataaggt
     4921 tagtttaaaa atggatctca ggacaggctt aggttatgat atacaccctc ttgtgcaggg
     4981 tagaccgctt gttgtagggg gtgttattat tgagaattca aaaaagctcg gacttgaagg
     5041 gcatagcgat ggtgatgttc ttacacatgc ttttatggat gccattatag gggcatgcgg
     5101 gtatggttcg ataggagagt ttttccctca ggagcctcaa tggaaggact caagaagtat
     5161 ttttcttttg aaggagcttt gcagggtgct gtcggggaaa gtaacgatag aaatatttaa
     5221 ttttgattgt atagttattt ccgatatagt aaagattacc cagtatggtc aggaaatgaa
     5281 agaaaatctt tcggatgcag ttggagtact tccccaaaaa ataaatatta aaggaaaaag
     5341 cggaaacgga gtagggtcaa gttcatcttc caaatcagta gaagcatatg tttccgtact
     5401 tttaagtatt gatagttccc ggagggaata ggcatgaatc agatgaaaac ccgtaattat
     5461 ctgtattttt tacatttttt aacttccata atcataaact cagttgctcc gcttatgacc
     5521 accatacagt ccgaatatac cgtcagtatt tctcagtcag ctcttgttcc tcttctcaat
     5581 acggcaggag ttctcatatc caactttgcc ggagggtttc tcatagcttc aatgggactt
     5641 aagacatcat tgcagctggg acttcttttc gggcttttgg gtacagctct tttcgctacc
     5701 tcatactcgt ttcttcaggt cctgatgggc gtatttttta taggaatgac aactggaacg
     5761 gttttcatga gtcttacctc tatttttgcc tctcttgatt caaagtatgc aaattatggg
     5821 ctttttcatg cgtttttcgg aataggcgga atcgtatcgc cccttataac cgcttttttt
     5881 cttaaagcga acttttcgta tcgaaatatt tttgcccttt atacagtggt tatggcttta
     5941 atccttgtat atacgtcgat gctcaagtat cttgaaaaca ccagacatcc ggctatacag
     6001 ccaaaggaag cccttggtat aatatcaagg cctttggttt atctgacact tttgatattt
     6061 ttgttgtatg caggaacgga gataggtata accacatgga gcgggaatct ttttgaaaaa
     6121 gctttccttc tcagcaaaag caatgctcag cttttactga gtaccttttg ggctgcgtat
     6181 acctttggaa ggataataac ggagtatatt gacagaaagc ttggcgctat aagaaccata
     6241 attctatctt ccgttatggt tattataagc atagcggcca tgcttacgat aaaaaactcc
     6301 gtaatgttca tttttatagg attattcctt ggacctatat tcccgaccgt tcagaaatat
     6361 gcaaattcta agctttctca gagggagatc gggctttaca gtggattatc atatggtttt
     6421 accggtttcg gatcaatgct tatagttacg tccatgggcc ttgcagccga tgttgataca
     6481 cgactgtctt atgtcatagc gctttttact tttactgtaa ttatttttat tgcagcagct
     6541 ttaggaagaa aaaaaggagg ccaggagaat gcaaaaaatt aggaaggttg tttatgtaat
     6601 gtttgtattt gttttcttat ttattctttc agggtgttct tcccaaccta aaaccaccca
     6661 gaatatgctt gatacaaagg gattggtaac tacaagagat ttcaaggatg taaaaggttt
     6721 tggcgtaata gactatacta aatttcccaa tccttcacag gcaatacttg cagcccatga
     6781 agcggctctg gtagacgcat atgccaaagc aacagagttt gtgtacggtc ttattgtgga
     6841 gggcggaagc tctgttgaga attttgcttt gaaagataag acagtagaaa ccagactaag
     6901 aggagtaata agaggcgctg aggagatttc gactgaaggc tcaaaggata cagatggtga
     6961 cggcatatat gccgtggtgt tgagaatata cagaaaagcc attgaagaaa ctttgggaat
     7021 gaagcttcag gcatggacat actgaagtag acacaataaa aattttcccc cgttaggggg
     7081 aaaatttatt gcttctgatc catcggagtt ccttttttcg cttcttttat tacatcatta
     7141 agagcctgtt gcagctgcac ctgctggtca cctgcttttt ttatgagctc tgtctgttca
     7201 agatagttaa taactgcctg aggattgtag aagatgctta cgtgatactc atacttattt
     7261 ccggtaggaa tgtagtattt gcataaaacc tttgctccct gctgtctgga tactgtaaat
     7321 gcttcctgta ccgtcttgcc tgccgaggca accatttcat ttacatcagc gttgttctga
     7381 gttgtcttta cttcgctgag gattccggaa actttggcag agtagccctg tacgattgta
     7441 tccagaaact ttgaaagttc tgttcttgca gcaagatcag ccatttcata gtacatgctt
     7501 ttgtcaacta ttccgccgga gtaagttcct tcaaactcta caaataagtt gttctgcaaa
     7561 agctccagga tcattttttc gtcataaggc atagggtata cgtcgattaa agcgtctttg
     7621 ggaatatact ttatggtggt agtaccgact ttcagcggct tcataccttc aggtactccc
     7681 gaagaacacg atgtaagtat caaagacata cataaaactg acaaaatcaa tgcaacaaac
     7741 cccttttttt tcataccggc cacctccgcc taatcttttg ctttactgat ttcttcattt
     7801 ataagtgtga aaagatcgtc aacgtcaata ccttccgtgt ccaaactgct tacagcattt
     7861 ttactttctt taattatctt gagtacagta actgggtcgt agaaaaccag gacatgatac
     7921 tcccgcaggc tcatagtgtc gatgtagtat cttcccaaaa ccatgcttcc cttaagctcg
     7981 ctttctgaaa gagcactttc taaagaagaa taaacggaaa tctcattctt cccgttcaga
     8041 taggttgtct ttgactttat ggattttacc tgtgtactca gaaattctga cagctgaact
     8101 ttcgctttat cttcggcctc cttataatgc tgatctgata gaaaccttcc tcctatcgcc
     8161 catgaattaa tcacataaaa cgagccggat tctgaaagtt ccgagactct gcggcttata
     8221 gagtagttta tatctgttat aacagcattc gggggaatca ttgcgacggt aacgtttccg
     8281 atgatgagag gcttaaaggc atcctgagat ttatctgctt tgtaatctgt ttttggttgc
     8341 agcgagcacg agctcaggaa aaatgttgac agaatcaaga agatcagaac ttttgtcttt
     8401 tgcatacagc accgcccctg aaatctaaaa aagcttaatc atactaatgc aaatatatta
     8461 taccataagc catgtgcaaa aatatcaaag aaaaataaac ttttcagacc tagagttttt
     8521 ttccgaaaga aacaactctt tctgcaaatg cccttttctt tagataagtt acaaactcag
     8581 gtatttcatt aagtacagga taagagtcca gcaatccggc gatgtagttt aaagaaaact
     8641 tatcaagttt ttctctgtta atagcatatc ctgaccttct ggaatagttt acgaatatat
     8701 atcttttcat tatgagatgt tcaacggact tattgagcat aagcatccct cctttttccc
     8761 ctctgttact aatgtaccat aaaaaagatc tgatttttta ggaactctga taaaaggggt
     8821 aaaaacgggt atgaaaagca aaaaaaaaat ttggcatttg ttaacatatt acttaagtca
     8881 caaatagtta aataataatg ttactgttgt gaattaagtc ataaaaaata atgacctatc
     8941 tcactaaaat tagtatgatt ttttgtctat aatctcaaag aaaagagata ttacagaagg
     9001 agggatatta tgaaaaaact attacttata gttttggcta ttgttactgt actgactgtt
     9061 tttggtgagc ctgaatggct tatagaaaat gttcagggcg gaaaaagagg gggaatactt
     9121 tatctttc
//
LOCUS       DAOSHR010000075         9118 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_6476_length_9118_cov_6.226084, whole genome shotgun sequence.
ACCESSION   DAOSHR010000075 DAOSHR010000000
VERSION     DAOSHR010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 9118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9118
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6476_length_9118_cov_6.226084"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1025)
                     /locus_tag="PLU28_05505"
     CDS             complement(<1..1025)
                     /locus_tag="PLU28_05505"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOG34556.1"
                     /translation="MRYDAIIVGSGMAGLTAGAYLCKNAHNVLIIEKSEKTGGLVTSF
                     ERDGFTFDAGIRAFENSGIIFPMLRQLGITVDFIKNPVSLGIEREFIYLKTKESIKDY
                     LELLLKNFPSQAEQIEQIINEIKKVMNYMDVLYGIDNPLFMDLKHDRDYLTKTLLPWM
                     IKYAKSIRKIEMLNMPIKEYLLNFTQNMQLIDTITQHFFADTPAFFALSYFGLYLDYS
                     YPKGGTQTLASKMTSFIRQNSGKILLKTEISQIDSERKKLFSSDSQEFEYNQLIWAAD
                     LKNFYRNLNKNSLKNLISNKKSEKTYNEIMNADASESVLTLYLALDLLKSFFASVIGA
                     HSFYTPQ"
     gene            complement(1338..2069)
                     /locus_tag="PLU28_05510"
     CDS             complement(1338..2069)
                     /locus_tag="PLU28_05510"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG34557.1"
                     /translation="MKILVFAKRNFKEIVRDPMNLFFGIGFPVLLLIALNTMGKNIPG
                     EQFTLKQLTPGITVFGLSFMSLFSGVLLAKDRSSSFLMRLFTSPLKSHDFIWGYLLPL
                     FPMAFIQGVICFIAAVLMGLDFNLNLVLSFITLIPTSFLFIGIGLVCGVIFNDKQVGG
                     ICGALLTNLTVWLSGAWFPVEIVGKTFKNVAMMLPFIHSVEISRDALNGSYQNFFIHL
                     IWILGYTVVFLSLAIFLFNRKMNKD"
     gene            complement(2066..2797)
                     /locus_tag="PLU28_05515"
     CDS             complement(2066..2797)
                     /locus_tag="PLU28_05515"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG34558.1"
                     /translation="MVDIKTENLTKKYRNLTAVDSIELSIEHGELFSLLGVNGAGKST
                     TIKMLTCLSKPTSGNAFILGESILTNPFFIKEKIGVSPQETAVAANLTVRENLEFISG
                     IHGSNNSLSKEKAAQMIKKFHFAKYENIRSKNLSGGWQRRLSIAMALISEPKILFLDE
                     PTLGLDVIARRELWDVISDLKATTTIILTTHYLEEAQALSDRIGIMSGGKLTAVGTVE
                     EIIKKSGKKNFEEAFITLAGEETKQ"
     gene            complement(2790..3038)
                     /locus_tag="PLU28_05520"
     CDS             complement(2790..3038)
                     /locus_tag="PLU28_05520"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HOG34559.1"
                     /translation="MQQGDKRFYGTVTVSERGQIVIPAEARKDFNIKTGDKLLVFGDL
                     NQGLAFATVEIMAKNMAGTTELFREIQNTIKPEEEQNG"
     gene            complement(3185..3559)
                     /locus_tag="PLU28_05525"
     CDS             complement(3185..3559)
                     /locus_tag="PLU28_05525"
                     /inference="COORDINATES: protein motif:HMM:NF018508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HOG34560.1"
                     /translation="MNLKQRVEKLKINIPAIFLALGKKETPVWAKILAWLTVAYALSP
                     IDLIPDFIPVLGYLDDLLILPVLVTLTIKLIPKDVFDQCLEQSKNIWKEGKPSRWYFS
                     IGIVILWIIIILIILKFFFFKT"
     gene            complement(3575..4261)
                     /locus_tag="PLU28_05530"
     CDS             complement(3575..4261)
                     /locus_tag="PLU28_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34561.1"
                     /translation="MSIVSEWNIDQKKMNELIKKESTFEAGKKILTALHTSVHFSIHG
                     GLTIMDDLWAGLEKSAFSIMPTEKDVTIAWNIWHITRIEDLTTNLLISGREQILNDQW
                     LKKIRTSVTDTGNAMTDDEIISFSREIDPCELRNYRVAVGERTKSILSGLNFKDMEKK
                     FDKKALAEILRVGGVTEHPDSIWLLDFWGNKSISGIIKMPLTRHQIVHINDSFRLKDT
                     IFKRKIFYLS"
     gene            complement(4312..5832)
                     /locus_tag="PLU28_05535"
     CDS             complement(4312..5832)
                     /locus_tag="PLU28_05535"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOG34562.1"
                     /translation="MRYKYNVITVVLIFIICLTVVYFYMISHKKAEKIFFDQTYDMIS
                     DLKKTFLKDTVDNLINSIDIELTNETRRYEKTGHVKFLELTSRLSLPEEEFLRYVSQQ
                     FFIQNDSKCLTYFIWNKNSGIVVSCSEDSFDKTVIYKIDELAENFYSYERIETTNSVV
                     LFGATKEYIDDIVKERMKNRIRTMTFKNDEYIWINEILNYDGGKNYALRLVHPNLPET
                     ENTFLSTDTKDDKGNMPYLMELEGVKNKGQVFFTYYFKKPSSGTSSEKISYARLYKKY
                     DWVIAMGTYTDDTHQYIEETERKIRDISLNVTLNTIWFLLAIVLVCLILMVAINRYYF
                     RRSNKDLEKEVNSDILTRTYSRRFGINEMNKAFSDFCKYGINSGIMVFDIDHFKEVND
                     TYGHDAGDMVLKELTNSVLSCIRSSDRCIRWGGDEFICVFKDIDAQAMNYFGQKILAA
                     VSSVSVHANGKEIFVNVSIGMSIFRKTDSTYDDALKRADRALYISKSNGRNQINVL"
     gene            6068..6979
                     /locus_tag="PLU28_05540"
     CDS             6068..6979
                     /locus_tag="PLU28_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34563.1"
                     /translation="MKCAKNATGWIGVFITLIISGVWSYWGAFENFHEGWYSKSIWEN
                     IFMLLFQYLLFAWVFTVLALISLRWKRTGLVVHLLTAVFSAWFFRGAVFSVVGLMVVI
                     PFFILAMLYYFGEPKPKKLAYILIIAVPLIIVTAISIPQAVRISRRVNDNDSGIRTIK
                     TLNYSLSWAPRGPGWPDRGVSWEEACKICMYLSADGETLASEPQNIWRLPTVEEAVSS
                     LSLRYNNSEGRWNKEEEKVYYKYVPDKESPLWDIYSKVIYYWTADTVPSDNECAYIIV
                     YNGGVYKRTKSSNQAYLSFRAVKEAAD"
     gene            7096..7557
                     /locus_tag="PLU28_05545"
     CDS             7096..7557
                     /locus_tag="PLU28_05545"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOG34564.1"
                     /translation="MDKKKVHTQLMEYYQIFKETDAIYNRLAKRAGLAESAYWVMYSI
                     RMMDGRCTQKEICSQWSLSKQTVNSGLKYLEKEGLIMLAEFSEDRRSKEILLTENGEK
                     FSKKYIDTVFEMEMETFEKMSDRERKGLIDSSRRYRDLLQKQADSFLDRQV"
     gene            7663..8994
                     /locus_tag="PLU28_05550"
     CDS             7663..8994
                     /locus_tag="PLU28_05550"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOG34565.1"
                     /translation="MNIRLSDHFTTGKLLRFTLPSVIMMVFTSIYSVVDGLFISRYVG
                     VEAMASISVVMPLLMVLGSFGFMLGSGGSAEVANTLGQGDVKKAKGYFTMLTISIFVI
                     GIVLSTSCLIFIRPICYFLGANDALIEHCVNYATICLIGNTFFMLQVFFQPFMITAEK
                     PHLGLLLTILGGVTNIFLDWLFIGVFHWGLRGAAVATLCGYLLSSIVPILYFLFPNKS
                     RLSFCKPKFYLKAFLHSAANGSSEMVSNISASITSFLFNTQLMRLAGETGVAAITIMQ
                     YVNFVFVAVLIGFSMGSAPVIGFHYGAGNIDELKNLFKKCLGIVLSVSVAMFIISEVF
                     SKPILRAFSSGSAVLEQMTFSGFRIFALSFIVSGINIFASSFFTALCNGKASAAISFL
                     RSLVLGAGMIMILPFVFGIEGVWMAPVVSEFFTMVFSVYLLKRHKKEYSYA"
BASE COUNT         2794 a         1586 c         1660 g         3078 t
ORIGIN      
        1 ttttgtgggg tgtagaagga atgggctcca ataacacttg caaaaaagga ttttaataga
       61 tctaaagcaa gatacagcgt taatacagat tccgaagcat ctgcattcat aatctcattg
      121 taggtctttt ctgatttttt attactgatt agatttttca aagagttttt attcagattt
      181 ctgtaaaaat ttttcagatc ggcagcccat ataagctgat tatactcaaa ctcctgcgaa
      241 tcagacgaaa atagtttttt tctttcagaa tcaatttgcg atatttccgt ttttaaaagt
      301 attttaccag agttttgacg aataaaagaa gtcatcttag atgcaagggt ttgcgttcct
      361 cctttgggat aagaataatc cagatagaga ccgaaatagc ttagtgcaaa gaaagctggg
      421 gtatctgcaa aaaaatgctg agttatcgta tcaataagct gcatattctg agtaaagttt
      481 agcagatact cctttatagg catattgagc atttcaattt ttctgatgct tttcgcgtac
      541 tttatcatcc aaggaagaag agtttttgtc agatagtccc tatcatgctt caaatccata
      601 aacaaaggat tgtctatacc atacaggaca tccatgtagt tcattacttt ttttatttcg
      661 ttgatgattt gctcaatttg ttctgcctga gaaggaaagt tttttagaag aagttcaaga
      721 taatctttta tactctcttt agtcttaaga tagataaact ctctttctat gcctaacgaa
      781 acaggatttt ttataaaatc aacggtaata cccagctgcc ggagcatagg aaatattatt
      841 cctgaatttt caaaggctct tattccagca tcaaacgtaa aaccatctct ctcgaaggaa
      901 gttacaagac caccagtttt ttctgatttc tcaataataa gtacgttgtg tgcgttttta
      961 caaagatatg ctcctgcagt cagacctgcc attcctgaac cgactatgat agcatcgtaa
     1021 cgcatatttt ctctccttag cagtagtcta atttttataa aagttatata atgttatttt
     1081 tatgcaaagt tttaagatga tttaataaca ttagacgatc taattataca ttgctaagat
     1141 gggaaatgtc aataaagtaa aatagagcag aatataaaga cttaaaaaaa actccatccc
     1201 tcatgataga agttcaatgg cagagtacct taatgttgaa aggagtgctt tgtcccgtga
     1261 actttcaaaa atgaagagag atggattgat tagctataaa aaaattactt tgttattttt
     1321 tgacttattt tactaggtca atctttgttc atctttctat taaatagaaa gatggctaga
     1381 cttagaaaaa ctactgtata accaagaatc caaataagat ggataaaaaa gttctggtaa
     1441 cttccattaa gtgcatctct tgaaatttct acagaatgga tgaaaggcag catcatagca
     1501 acatttttaa aagttttacc tacgatttca acaggaaacc atgcaccgct tagccatacg
     1561 gtaagatttg taagaagtgc gccacaaatt ccgccaacct gtttgtcgtt gaatataact
     1621 ccgcagacca gccctattcc tataaaaaga aaggaagtcg gaataagagt aataaaagac
     1681 agaactaaat tcaaattaaa atcaagtccc atcagcacag cggcaataaa acaaatgaca
     1741 ccctgtataa aagccatagg aaatagggga agcagatatc cccatataaa atcatgtgat
     1801 tttagaggag aagtaaacag acgcatcaga aatgagctgc ttcggtcttt tgccagcagt
     1861 actcctgaaa aaagcgacat aaaagagaga ccgaaaacag ttatgcctgg agtaagctgt
     1921 tttaaagtaa actgttcacc cggtatattt tttcccatag tattgagtgc tattagaagg
     1981 agtacaggaa aacctattcc aaaaaagaga ttcatgggat ctcttacaat ttctttaaaa
     2041 tttctttttg caaagacaag tattttcatt gtttggtttc ctccccagca agtgttataa
     2101 atgcttcttc aaaattcttt ttcccactct ttttaattat ctcttcaact gtaccaactg
     2161 ctgtcagctt accgccggac ataatcccga ttctgtctga aagagcctgt gcttcttcaa
     2221 gataatgtgt ggtaagtatt atggttgttg ttgctttaag atcacttatt acatcccaaa
     2281 gctctctgcg tgcaataaca tcaagtccca gagtaggttc gtcaagaaag agaatctttg
     2341 gttctgagat aagagccatg gctatactga gcctacgctg ccatccgcca gaaagatttt
     2401 tggaacgaat attttcatac ttagcaaaat gaaatttttt gatcatctga gcggcttttt
     2461 ccttggataa gctgttgtta ctgccgtgaa tgccagaaat gaactcaaga ttttcccgta
     2521 cggttagatt tgcagcgaca gctgtttcct gcggggaaac tccaatcttt tcttttataa
     2581 aaaagggatt ggttaaaata ctttcaccta aaataaatgc gttacctgaa gtaggcttac
     2641 ttaagcatgt aagcattttt atggttgtgc tctttccagc tccatttact cctaaaagag
     2701 aaaaaagttc tccgtgttct attgaaagct caattgaatc tactgcggtt aggtttcgat
     2761 actttttagt caagttttct gttttaatat caaccatttt gctcttcctc cggttttatg
     2821 gtgttttgta tttctctgaa aagttctgtt gtccctgcca tattttttgc cattatttca
     2881 actgtggcaa acgccagtcc ttgattgaga tctccgaata caagaagctt atctcctgtt
     2941 tttatgttaa aatcttttct tgcttccgct ggaattacta tctgtcctct ttcacttact
     3001 gtaactgttc cataaaatct tttatctcct tgctgcatga taacctcctt ctttttttgt
     3061 aaatcttgtt tttcttacta tacttatata ctaacaaatt cttgtataca aatcaatgaa
     3121 gactgaacta catatctatt aagttcatat aaataaaaaa cagactcagt cagaagtctg
     3181 tcttttaagt tttaaagaaa aaaaatttca atataatcaa aattattata atccacagaa
     3241 taactatacc gattgagaag taccatctac tgggttttcc ttctttccag atgttttttg
     3301 attgctctag gcattgatca aaaacatctt taggaattaa ttttatcgta agcgttacta
     3361 aaacaggcaa tatcagcaaa tcatccagat agcccaaaac aggtatgaag tcgggaataa
     3421 gatctatggg ggataaggca tatgcaactg ttagccaagc taatatctta gcccaaacgg
     3481 gtgtttcctt ttttcctaag gcaagaaaaa tagccgggat attaatttta agcttttcta
     3541 cgcgttgctt gagattcatg aggttcacct tcagtcaaga aagataaaat atctttcttt
     3601 taaaaatagt atctttgagt ctgaaactat cgttgatatg gacaatctgg tgacgggtca
     3661 aaggcatttt aataattcct gaaatactct tattgcccca gaaatccaaa agccaaattg
     3721 aatcaggatg ttctgtaact cccccaactc ttagtatttc agcgagagct tttttatcaa
     3781 attttttctc catatcttta aagtttagtc cggacaaaat acttttggtt ctttcaccaa
     3841 cagctactct gtaatttctc agctcacaag gatctatctc tctgctgaaa ctaattattt
     3901 catcgtctgt cattgcatta ccagtgtctg tgactgatgt tctgattttt tttagccatt
     3961 ggtcattaag gatttgttct ctgcctgaaa tgagtagatt tgttgttaaa tcctcaatcc
     4021 ttgtgatatg ccagatgttc caggctatgg ttacatcttt ttctgtaggc ataatgctga
     4081 aagcactctt ttcaagacca gcccagaggt catccatgat agttaagccg ccatggattg
     4141 aaaagtgtac tgatgtatgc agagcagtca agattttttt cccagcttca aaagtacttt
     4201 ccttttttat tagttcattc atttttttct gatcaatatt ccattctgat actatactca
     4261 taattaatgc ccccggagta caaataaaaa aatctgatta ttgcaattct atcataaaac
     4321 attaatctgg tttcttccat ttgattttga aatgtaaaga gctctatctg ccctctttaa
     4381 tgcatcatca taagtactgt cagtttttct gaaaatagac attccgattg agacatttac
     4441 aaagatttcc ttaccgtttg catgtactga gaccgaagaa acagcagcaa gtatcttttg
     4501 tccaaagtaa ttcatggcct gtgcatcaat atctttgaaa acacatataa actcatctcc
     4561 gccccagcgt atacatcggt ctgaacttct tatacagcta agcacagaat ttgtcagctc
     4621 cttcaaaacc atatctccgg catcgtgtcc gtaagtatcg tttacttcct tgaagtggtc
     4681 tatatcaaaa accattatgc cgctgttgat accatactta cagaaatcag aaaaagcttt
     4741 attcatttca tttattccaa atcttctact gtaggtacgt gtaagaatat cactgtttac
     4801 ttctttttcc agatccttgt ttgatcttct gaaataatat ctgttgattg caaccatcaa
     4861 gatcagacat accagaacta ttgccaataa aaaccatata gtattgagag tgacgttaag
     4921 agatatatcc cgtatcttgc gctcggtttc ttcaatatac tggtgcgtat cgtcggtata
     4981 ggtgcccatg gcgattaccc agtcatattt tttataaagc cgtgcgtagg aaatcttttc
     5041 tgaggaagta ccgctactgg gttttttaaa gtaatacgta aagaatacct gtcctttatt
     5101 ttttactccc tcaagctcca taagataagg catattgcct ttgtcatcct tcgtatctgt
     5161 agaaaggaaa gtattttcgg tttccggaag attgggatga acaagtctta aggcatagtt
     5221 cttccctccg tcgtaattta aaatttcgtt gatccagata tactcatcgt ttttgaaggt
     5281 catagttctt attctgtttt tcatccgttc ctttacaata tcgtctatgt actctttggt
     5341 tgcaccgaaa agtactactg aatttgtagt ttcaattctc tcgtaggaat aaaaattttc
     5401 tgcaagctca tctattttgt atataaccgt tttatcaaaa gaatcttcag agcatgatac
     5461 cacgattcct gaatttttgt tccagataaa gtatgtgaga catttagagt cgttttgtat
     5521 aaaaaactgt tgtgatacgt accttaaaaa ctcttcctca ggcagagata atctggaggt
     5581 tagttctaaa aattttacat gaccggtttt ttcataacga cgtgtttcat tagtcaattc
     5641 aatatctatg ctatttatta aattatcaac cgtatctttc agaaaagttt ttttaagatc
     5701 tgatatcata tcatatgtct ggtcaaaaaa gattttttct gcctttttat gtgatatcat
     5761 gtaaaaatat accactgtta agcagattat aaaaattaag accacagtaa ttacattata
     5821 cttatatctc ataacaggtt ttcctcctgt atatatgaaa aaatcaaaaa cattgcatca
     5881 atgcaaagct gttgtaaata taatacactc cgtattaaag tagagtgcac tgtaaaaagt
     5941 tgatactatt tctacagcaa tagtatattt tgttcgttaa gttataaggt attgagtaaa
     6001 aatagtataa agataatttt aaaagcatat aattaataca agctatcttt tattttcgga
     6061 gggcaaaatg aagtgtgcaa aaaatgcaac tggctggatc ggtgttttta taactttaat
     6121 tatttcagga gtatggtcat actggggagc gtttgaaaac tttcatgaag gttggtactc
     6181 aaaaagcatt tgggagaata tctttatgct gttatttcaa tatcttcttt ttgcgtgggt
     6241 atttactgtt ctggcactta tcagcttaag gtggaaaagg acaggtctgg tagttcatct
     6301 gctgacggct gtattcagtg cttggttttt cagaggcgca gttttttctg tggttggact
     6361 aatggttgtc attccttttt ttattcttgc aatgctatat tattttggag agcctaaacc
     6421 aaaaaaactt gcttatatac ttataatagc tgttcctttg attatagtta cggctatatc
     6481 tattccacag gcggtaagaa tttcaagacg ggttaacgat aatgattcag gcataagaac
     6541 aattaaaact cttaattact cgctttcatg ggctcccaga ggtccgggat ggcctgacag
     6601 aggagtttca tgggaggaag catgtaagat atgcatgtac ctttcagcgg atggagaaac
     6661 ccttgcatct gaaccgcaaa atatatggcg gcttccgaca gtagaggagg ctgtaagttc
     6721 gttgtctttg cgttataata attctgaagg aagatggaat aaggaagaag aaaaagttta
     6781 ttataaatac gttccagata aagagtctcc tctttgggat atttattcta aagtaatata
     6841 ctactggaca gccgatactg tcccaagcga caatgagtgt gcatatatta tagtatacaa
     6901 cggtggtgta tataaaagaa ccaaaagcag taatcaggca tatctcagct tccgcgcggt
     6961 gaaagaagct gctgattaag ttgtttttac agcatattta attcaaaaag caaagtaatc
     7021 tattgatgtt gacaattttt aataagtata gtatactata ttttagtccg aaataggact
     7081 gtctgggagg acaacatgga caaaaaaaag gtccatacgc agttaatgga gtattatcag
     7141 atatttaagg aaacagatgc aatttacaat agacttgcca aaagagcagg tcttgccgaa
     7201 agcgcttatt gggtgatgta ttcgattaga atgatggacg gaagatgtac tcaaaaagaa
     7261 atatgcagcc agtggtcgct cagcaagcag acggttaatt ccgggcttaa gtatttggag
     7321 aaagaaggac tcataatgct tgcagagttt tcagaagaca gaaggagcaa agaaatttta
     7381 ctgaccgaaa atggggaaaa attttctaag aaatatattg atactgtttt tgaaatggaa
     7441 atggaaactt ttgaaaaaat gtccgacaga gaaagaaaag ggctaataga tagctcaaga
     7501 agatataggg acttgttaca aaaacaggcg gattcatttt tggataggca ggtttgaaca
     7561 tcggaggata ttaatcgcaa ttaataaacc ggataagatg ctgagtgcgc tcagtctctt
     7621 atccgatttt ttaaaggtta acttaaatac ggaggtattg ttatgaatat aagactttca
     7681 gatcatttta ctacaggaaa acttctcagg tttactttgc catctgtcat catgatggtt
     7741 tttacttcta tctatagtgt tgttgacgga ctgtttatct ccagatacgt aggggttgag
     7801 gcaatggcct cgataagtgt agttatgcct ttactcatgg tattaggctc atttggattc
     7861 atgcttggtt ctggaggcag tgcggaagta gctaatactc ttggacaggg agatgtcaaa
     7921 aaagcaaaag gttacttcac tatgctgaca ataagcattt tcgttattgg aatagtcctt
     7981 tcgacatcat gtcttatttt tataagaccg atatgttatt ttctgggagc aaatgatgct
     8041 cttatagaac actgtgtaaa ctatgcaacc atatgtctta tagggaatac tttttttatg
     8101 ctccaagttt tcttccaacc atttatgatt actgccgaaa agccacatct aggacttttg
     8161 cttaccatat tagggggagt aaccaatatt tttcttgact ggcttttcat tggggtattc
     8221 cattggggac ttcgcggtgc agcggtagcg acattatgcg gatatctttt aagcagtata
     8281 gttccgatac tgtatttttt atttcctaat aagagcagat tatccttctg caagccgaaa
     8341 ttctatttaa aagcttttct ccatagtgct gcaaatggtt catcagaaat ggtctcaaac
     8401 atatcagcat caataacgtc tttccttttt aatactcaat tgatgaggtt ggcgggtgaa
     8461 acgggagttg cagccataac cataatgcag tatgtaaact ttgtttttgt tgcggtactg
     8521 atagggtttt caatgggaag tgctcctgtt ataggctttc attatggagc aggcaacatc
     8581 gacgagctta agaatctttt caaaaaatgt ttaggaatag ttttgtcggt atcagtcgcc
     8641 atgttcatca tatctgaagt tttttctaaa ccgattctaa gagcatttag ctccggatcg
     8701 gctgtgctcg agcagatgac gttttctgga tttaggattt ttgctttgtc ttttatcgta
     8761 tcagggatta atatatttgc ttcttccttt tttaccgccc tttgcaacgg gaaagcctcc
     8821 gcggcaatat ctttcttgcg ttctttagtt ctgggagcag gtatgataat gatactgcct
     8881 tttgtttttg gtattgaagg agtttggatg gcaccagtgg tttcagaatt ttttacgatg
     8941 gtattttcag tatatctttt aaaaagacat aaaaaagagt acagttatgc atagagtagt
     9001 atcaaaagac agttctcact tcaagttgaa gtgagaactg tcttaaaatg ctagaacttg
     9061 tattgtttta acattaattc gagttttgag gatagctgtg ccaatgactc tgctgatg
//
LOCUS       DAOSHR010000076         9066 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_6528_length_9066_cov_5.407946, whole genome shotgun sequence.
ACCESSION   DAOSHR010000076 DAOSHR010000000
VERSION     DAOSHR010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 9066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9066
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6528_length_9066_cov_5.407946"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1082)
                     /locus_tag="PLU28_05555"
     CDS             complement(<1..1082)
                     /locus_tag="PLU28_05555"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOG34566.1"
                     /translation="MVKKLYSQAQADPENKNSLKEGNELTITGFAFEKFERKGLRYIY
                     ATDYTKSYVILCKCPFEPDIPSIIRFRGTFFYDEHIGDYVIAADEIYPDSDFYPRDEA
                     KEKRVELHIHTKMSAVDSTVDISAASELIKNWGHDAAAVTDSNCLHAYPEFEKASEIY
                     GFKPIYGCEINITEKNKVYSTTIIVRNRKGLKNLYRIITDAHLNAVSRVPITTFEKLS
                     SFREGLLISAGSLKSIVAELYTCQEYEQAKERMEFFDYLEVFPILAVTDKRFNTQKLK
                     EYYNFLYDCASEMKKPLVMSGDVHYLRKEDKKLYDALVYGTRTAKNDRKIINSERFLR
                     TTADMFSQAMDIFDDEKKAYLTTITNS"
     gene            complement(1207..2235)
                     /locus_tag="PLU28_05560"
     CDS             complement(1207..2235)
                     /locus_tag="PLU28_05560"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOG34567.1"
                     /translation="MKKFLTFAFFVLAVLLVLTSCNKQPEKLYVYNWANYIPQEVVQS
                     FEKEYNCDVVYDFYSSNEEMYAKLKAGGTGYDIIFPSADYAQIMINENMLEKLDKAKV
                     PNLSNIEQSSLMKMSYDINQQYCLPYMIGSTGLAVNTKYVKEYDHSWTIFERSDLKGK
                     MSLLDDMRETLGAALKFLGYSVNTVKEAELEQAKQLVLKWKENILKFDGLSNAKNYAN
                     SEYYVIHGYGDSIFLELDEEMLKNTDYFIPKEGGTLWIDNMVMLKNSKHKELAYKFMN
                     YIYEPKIYAQISDYVQLPSINIPAREFITVQPMYKEGELDNCELIMDLGKDIELYNKV
                     WQEIRMQN"
     gene            complement(2253..3029)
                     /locus_tag="PLU28_05565"
     CDS             complement(2253..3029)
                     /locus_tag="PLU28_05565"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG34568.1"
                     /translation="MKEKHRFSFTMFILTMIFFYLPIAVLIIFSFNEGKTMAWSGFSL
                     KWYQKLFFESEELWKAVFNSVIIAIFSSLVSTAIGTLGAIGMYWYRFRSKKYIQVASY
                     LPLIMPEIIMGVSLLIFFVMFKIPLSLLTIFIAHTTVSIPYVLFIVMARLEEFDYSII
                     EAAYDLGAKEIDTLFKVILPISMPGIISGFLMAVTLSLDDFVITFFVSGPGSSTLPLY
                     INSMIRFGVSPIINAVSVVLIASTILLAVSTRRFYKYMFS"
     gene            complement(3026..3904)
                     /locus_tag="PLU28_05570"
     CDS             complement(3026..3904)
                     /locus_tag="PLU28_05570"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG34569.1"
                     /translation="MKKNYGPVYSLPLGLWITIFFIVPTCIIFIYSFLTKATYGGVQW
                     QFSLEGYKALANTSFVNVVWITVYISVISTFLTVLFAIPTAYYIARNKHKNQLLLLVI
                     IPFWTNFLIRVYAWIAILGNNGFVNNILVKLGVIDTYIQFLYNQYAVILVIIYTYLPY
                     AILPLYSSIEKFDFSLLEAARDLGATKAQAFFKVLLPNIKGGLATSILFTFIPALGSY
                     VIPMLVGGKDSMMLGNIISRELTTTRNWPLASSISVVLTVITMIGVLLFLKLNKNNLE
                     SIKKLVVREKAGEESI"
     gene            complement(3901..5007)
                     /locus_tag="PLU28_05575"
     CDS             complement(3901..5007)
                     /locus_tag="PLU28_05575"
                     /inference="COORDINATES: protein motif:HMM:TIGR01187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG34570.1"
                     /translation="MKGSEIRVENVTKRFGDFVAVENASFEIQRGEFFSLLGPSGCGK
                     TTLLRIIAGFEDPSEGIIYLDGQNLIKILPNERPVNIIFQNYALFPHLSVFENVAFSL
                     RLLKISESEIKRQVEKYLSLVKLDMHTKKYPSQLSGGQKQRVAIARALIGEPKVLLLD
                     EPLSALDAKLRQHMLIELDSIHDEVGITFIYVTHDQQEALSVSDRVAIMNDGEVLQIG
                     TPHQIYESPADSFVASFLGETNFLRGRVNQVNEEYAKIEIKELGELFVYLDKPLKIND
                     VVKLTIRPEKIRITKQMPENLPDKFNVLHGRVDEVIYSGFQSKYFVKSGKFSFKVYKQ
                     HVNYLLDETPIKWNEDVFIWWNVDDGFIVEVEEE"
     gene            5288..6004
                     /locus_tag="PLU28_05580"
     CDS             5288..6004
                     /locus_tag="PLU28_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34571.1"
                     /translation="MDNEFEGSFEQDFDGFMRELFKNNPELKNFNLDMLKSMDKEQSA
                     ELIQKLVSASKKFVDAEKDVQQRVADKIDFEPKDLIIDFDNYIKTCMNFPFAITVTED
                     VLDNEDENGVLKGQFFGKNLAIPYSNIYELISIKKLISMHFAKLIRTNINSFLPIRSE
                     LYSYISNSTKDFLKSLELEELFFIKDIREFNMIIELKNKKYDSIIEFYNDPQLEDENE
                     KLRNMKLLLLTEFAIIVIES"
     regulatory      6016..6124
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6180..7157
                     /locus_tag="PLU28_05585"
     CDS             6180..7157
                     /locus_tag="PLU28_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HOG34572.1"
                     /translation="MKKWLVLAVLLIGIAAFSVSQLNVYVYESLNWIEDKLIGKFESS
                     NNCKVNVVRLGDAGNMVARLKLEKKNPKADVVIGLDQSLSMSAISEGLLQPYISPMLS
                     KLRDPNLIFDKDYYVTPFDYGAIAFVYDPSKLSKIPEKMEDISAYKNSLIIQDPKSSS
                     TGQALLIWTIALYNDSWTDFWKKMKPAVLTVTPSWDDAFSKFEQGEAPIMLSYATDGA
                     YSYHYYQSTKYKAFIPKDGAYVQIEGAGIVKGSKNKQLAQKFIDFLLSDEFQQEVALN
                     QWMFPVTDVKLPEAFKYAVVPEKILSVDIKAISSQMDKYIKTWEEIMYK"
     gene            7154..8713
                     /locus_tag="PLU28_05590"
     CDS             7154..8713
                     /locus_tag="PLU28_05590"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOG34573.1"
                     /translation="MKLSIKKIPGSIPGIVYVLAVLTAVFPIFKDFFNLTSFSALQDS
                     QTMRILFFTFYQALLSSIASLVVSIIPAYYSWRKKNILSAMIDLSFFIPFFFPVVSMT
                     IAFTALYGRSGLFFALFPGVSLSYSLIGIVVAHVFYDSPIFVKYISEGLRSVPQSLCE
                     SAQMDGASKTVIFFSIQLRLILPALVKAFLLVFTYCFMSFGVILSIGGMKFSTVEVAI
                     SSTISSSFDFSKAIVLCAVQFLVIFLLNYTGSKFTSFEGSVQTYPSSKRTALFTGVFS
                     FIYVLFEYSLIVLSLAGGFFNFQASRFDLSGLINLFSGPVNAKYGISASLINSSSVSL
                     ITALICTVLAYIMLRVSSRFSEVIILSCLGISPAFISIALLYLNISWLVPYPLLLVFG
                     YVLLNMPIAFSFMYQPVKAFPSVITEAAKMDGATSVSIFSRIHFPLMYRIFVSSFLQI
                     FAIIFGEFTMIYTMQIQKTFPMVSITNYILSSNRMFKESNALSSLSIFIVISVFFFSK
                     ILEKKHRSLLE"
     gene            8735..>9066
                     /locus_tag="PLU28_05595"
     CDS             8735..>9066
                     /locus_tag="PLU28_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34574.1"
                     /translation="MKKKHFFLWIFLLLLVLAVVFTLLFILRRPEEKVSVLLYHRFND
                     SRYPSTSVDSAELENQILFLKSRGVSFLSENDFISLLEGKKKYGNSVLISIDDGFRSI
                     LTAYPVLK"
BASE COUNT         2657 a         1737 c         1633 g         3039 t
ORIGIN      
        1 gagtttgtta tagtagtaag gtaagctttt ttttcatcat cgaatatatc catagcctga
       61 ctgaacatat ctgccgtcgt tcttagaaat cgttcgctgt taataatttt tctgtcattc
      121 tttgcggttc ttgtaccata aacaagggca tcatacagtt ttttatcttc tttgcgcagg
      181 taatggacat ccccgctcat aaccagaggt tttttcatct ctgatgcaca gtcatacaga
      241 aagttatagt actctttaag cttctgcgta ttaaatcttt tatctgttac tgcaaggata
      301 ggaaatactt caaggtaatc aaaaaattcc attctctctt ttgcctgttc gtactcctgg
      361 catgtataga gctcggcaac aatagatttt aaacttcctg cagatatcaa aagtccttca
      421 cgaaaagatg acagcttttc aaaggtagtt atcggcactc gtgaaactgc attaagatgt
      481 gcatcagtta ttattctata aagatttttg agtccttttc tgtttcttac tatgatagtt
      541 gtactgtata ctttgttctt ttcggttatg tttatttcac agccgtaaat cggtttaaaa
      601 ccatagatct ccgaagcttt ttcaaattcc ggatatgcat gtagacagtt gctgtctgtg
      661 actgctgctg catcatgtcc ccagttttta ataagttctg aagcagctga tatgtcaact
      721 gtagagtcta cggctgacat ctttgtatga atatgaagct ccactctttt ttctttagct
      781 tcgtcacgcg gataaaagtc actatcggga tatatttcat cggcagcaat tacatagtct
      841 cctatatgtt catcgtaaaa aaaggtgccc ctgaaccgga tgatacttgg tatatccggt
      901 tcaaaagggc atttgcaaag aatcacataa gacttggtat agtcggtggc atatatgtac
      961 ctcagacctt tgcgctcgaa tttttcgaac gcaaatcctg ttattgtaag ttcattacct
     1021 tcttttaaac tgtttttatt ttccggatca gcttgagcct gagaataaag ttttttgacc
     1081 atataatcac cttatacaaa tgtatacctt tattgtacat gatatgtttt tcatttatgt
     1141 ttcataaagc ttaaaaaaac aaccggactt tcaatgtatc ttgaaagtcc ggttgcaatt
     1201 ttcggtctag ttctgcattc ttatttcctg ccacactttg ttgtaaagtt ctatatcttt
     1261 tcccagatcc attataagtt cacagttatc aagttctccc tctttataca tgggctgcac
     1321 cgttataaac tccctggcag ggatgtttat ggagggaagt tgtacgtaat cgcttatctg
     1381 ggcatatatt ttaggttcat atatgtagtt catgaattta taagctaatt ctttgtgttt
     1441 actgttttta agcataacca tattatctat ccaaagtgtt ccgccttctt tgggaatgaa
     1501 ataatctgta tttttaagca tttcttcatc aagctcaagg aagatactgt caccatagcc
     1561 atgtatcacg tagtactcag agttggcata gttctttgcg ttggaaagtc catcaaactt
     1621 gagtatattt tccttccact ttaatacaag ctgttttgcc tgttcaagct cagcttcctt
     1681 tacggtattt accgagtacc ccaaaaactt tagagctgca ccgagggtct ctctcatatc
     1741 gtctaaaaga gacatctttc ctttgagatc tgaacgttca aagatagtcc agctatggtc
     1801 atactctttt acatacttgg tgttgaccgc aagcccagta gagcctatca tatatggtaa
     1861 acagtactgc tggttaatgt cgtatgacat cttcataaga gatgactgtt ctatatttga
     1921 gaggtttggc accttagctt tatctagttt ttcaagcata ttttcattta tcatgatctg
     1981 ggcatagtcg gcagaaggaa atattatatc ataacctgtg ccgcccgctt taagcttagc
     2041 atacatctct tcattcgaag agtaaaaatc ataaacaaca tcgcagttgt actctttctc
     2101 aaagctctgt acaacttcct gaggaatgta gttagcccag ttatatacgt ataacttttc
     2161 tggctgttta ttacagcttg taagaactaa aagaactgcc agaacaaaaa aagcaaaagt
     2221 taaaaacttt ttcacttttt aaatcctcct ttttaagaaa acatatattt gtaaaacctt
     2281 cttgttgaaa cagcaagaag tattgtgctt gctataagaa ctacagaaac agcatttata
     2341 atcggtgaaa ctccaaagcg tatcatcgag tttatataca gcggtaatgt tgatgaacca
     2401 ggtcctgaaa caaaaaaggt tatcacaaaa tcatccagcg aaagagtaac agccataagg
     2461 aaacctgata ttatgcccgg catcgatatg ggaagaatga ccttaaaaag tgtgtctatt
     2521 tctttagctc ccagatcata ggcagcttca attatagagt agtcaaactc ttcaagtctt
     2581 gccatgacta tgaacagcac atacggaata cttaccgtgg tatgggctat aaagatggtc
     2641 agaaggctca gcggtatttt gaacataaca aagaatatta aaagggaaac acccattatt
     2701 atctctggca ttataagagg aagatatgac gcaacctgga tgtatttctt acttctgaac
     2761 ctgtaccagt acatgcctat agctcccaat gtccctattg cagtggatac gagggacgag
     2821 aaaatcgcaa ttatcacact gttgaaaacg gccttccaca gttcttcaga ctcaaaaaaa
     2881 agcttctgat accatttaag agaaaacccg ctccaagcca tcgtttttcc ttcgttaaat
     2941 gaaaaaatta tgagaacagc tatcggaaga tagaaaaata tcatggtgag aataaacata
     3001 gtaaatgaaa atctgtgttt ttctttcata tggactcttc acctgctttc tctcttacaa
     3061 caagcttttt aatgctctca aggttatttt tattaagctt tagaaataag agtactccaa
     3121 tcatcgttat caccgtaagc acaactgata tagaggaggc cagcggccag ttccttgttg
     3181 tggtaagttc acgtgatatt atgttgccaa gcatcatgga gtcttttcct cctacaagca
     3241 tcggtattac atacgaccct aaagcaggaa tgaatgtaaa aagtatagag gttgcaagac
     3301 ctccctttat attgggaagt aacaccttaa aaaaagcctg tgccttggtt gctcccagat
     3361 ctcttgctgc ttccagaagt gaaaagtcaa acttttcaat agatgagtaa agcggcagta
     3421 tggcatatgg aaggtatgtg tatattataa ccagaattac tgcatactgg ttgtatagga
     3481 actgtatgta tgtatctata acccccagct ttacgagtat gttgtttaca aatccgttat
     3541 ttcctaatat ggcaatccaa gcatatacac ggataagaaa gtttgtccag aacggtatta
     3601 ttacaagcaa aagaagctgg ttcttgtgct tgttccttgc gatatagtac gctgtgggaa
     3661 tggcaaaaag aactgtaaga aaggttgaaa ttacagatat ataaaccgtt atccatacaa
     3721 cgtttacaaa gctcgtgttg gcaagcgcct tgtatccttc aagggaaaac tgccattgga
     3781 caccgccgta agttgctttg gttaggaagc tgtatatgaa tattatacag gtgggaacta
     3841 tgaaaaatat ggttatccaa agtcctaaag gcaaagaata aaccgggccg tagtttttct
     3901 tcattcttcc tcgacctcga ctatgaaacc gtcatcgaca ttccaccaga taaaaacatc
     3961 ttcattccac tttatgggag tttcatccag aagataattt acatgctgtt tatatacttt
     4021 aaaactgaac ttgcccgatt ttacaaagta tttgctctga aatcctgaat atattacttc
     4081 gtctactctt ccgtgcagaa cattaaactt gtccggaaga ttctccggca tctgtttggt
     4141 tattcgtatc ttttcaggtc ttattgtaag ttttaccacg tcatttattt taaggggttt
     4201 gtcaagatat acaaaaagct ctcccaactc ctttatttct attttggcat actcttcatt
     4261 tacctggttc acgcgtcctc gcagaaagtt ggtctcacca agaaaacttg ctacaaatga
     4321 atcagccgga ctttcgtata tctgatgggg agtacctatc tggagaactt caccgtcatt
     4381 cattatggca accctgtctg atacactgag cgcttcctgc tgatcgtgag taacgtatat
     4441 aaaggttatg cccacttcat cgtgaatgga atcaagctca ataagcatat gctgtctgag
     4501 tttggcgtca agagcactca aaggttcatc taaaagtaaa accttcggct ctcctataag
     4561 tgctctggct atggcaactc tttgtttctg cccgccggaa agctgcgaag gatatttttt
     4621 ggtgtgcata tccagtttaa caagtgaaag atacttctct acctgtcttt ttatctctga
     4681 ttcgcttatc tttaaaagcc tgagggaaaa agcgacattc tcaaaaactg ataaatgggg
     4741 aaaaagcgca tagttctgaa aaattatatt cacaggtctt tcatttggca agatctttat
     4801 aagattctga ccgtcaagat atattatacc ttcactcgga tcttcaaaac ctgctataat
     4861 ccttaaaaga gtagttttac cgcaaccgga cgggcctaat aacgaaaaaa actctcctct
     4921 ctggatttca aaacttgcgt tttctacggc aacaaaatcg ccaaatcttt ttgttacatt
     4981 ttctaccctt atctcgcttc ccttcaactt tccagcctcc tttaccgtaa tttccaaaac
     5041 tgcttcgctg ctgttgataa gcgaagttcc aaactgcttt tttgtaccag aatattatac
     5101 attaatttta actttttttg ttttcctaat tatttctttt agttaagaat actgcaatcc
     5161 attcagattt agacttcaga cgttaaaata tacaacaaaa catataataa caagaaagaa
     5221 aaaagtaaca ttattttcct ttttgtgtag tataattttt tttactataa gatcaaggag
     5281 cataaaaatg gataatgaat ttgaaggaag tttcgagcag gattttgacg gttttatgag
     5341 agaactcttc aaaaacaatc ccgaactgaa aaattttaat cttgacatgc ttaagtctat
     5401 ggataaggaa cagtcagcag agcttatcca gaagcttgtt tctgcttcaa aaaagtttgt
     5461 tgatgctgaa aaagatgtac agcaaagagt tgcagacaag attgattttg aacccaaaga
     5521 tctgattata gactttgaca actatataaa aacctgtatg aactttccct ttgccataac
     5581 ggtaactgag gatgttctgg acaacgaaga tgaaaacggt gttcttaagg ggcagttctt
     5641 cggcaaaaac cttgctatac cttattccaa tatatatgaa ctgatatcta taaaaaaact
     5701 tatttcaatg cattttgcaa agcttatacg cacaaacatc aacagttttc ttcctatacg
     5761 ttcagaactg tactcttata taagcaacag tacaaaagat tttctgaaga gtcttgaact
     5821 tgaggagctg tttttcatca aagatatacg tgagtttaat atgattattg aactgaaaaa
     5881 caagaaatat gactctatta ttgaatttta caacgatccg cagcttgaag atgaaaatga
     5941 aaagctacgt aatatgaagt tgcttttact gacagaattt gctataatcg ttattgaaag
     6001 ttaaagcagt acctgtttat acgccagggg agctggttac ctaacagctg agaggatgta
     6061 cgaaacatcg accctgacac ctgatccgga caataccggc ggagggaggc gttgatgaaa
     6121 aatatcaaat gtatccctcg tttatcgagg gattttttat tttataagga ggtatttaaa
     6181 tgaaaaaatg gttggtactg gcagttcttc tgatcggcat agccgcattt tctgtctcac
     6241 agcttaatgt ttatgtatat gaaagtctta attggattga ggataagctg ataggaaagt
     6301 ttgaatcatc aaacaactgt aaagtaaatg tagtaagatt gggtgatgca ggaaacatgg
     6361 tagccagact gaagcttgaa aagaaaaatc caaaggcaga cgttgtaata ggtcttgacc
     6421 aatctctcag catgtcggcc atatcagaag gtcttttgca gccttacata tcgcctatgc
     6481 tttcaaagct gcgcgatcca aaccttatct ttgacaagga ttattacgtt acaccttttg
     6541 actatggagc aattgctttt gtctatgatc cttcaaagct ttcaaaaatt ccggaaaaaa
     6601 tggaggatat ttcggcttat aaaaactctc tcataatcca ggatcctaaa agctcaagta
     6661 caggacaggc acttttaata tggactattg ccttatataa tgattcttgg acggactttt
     6721 ggaaaaaaat gaagccggca gttcttacgg ttactcccag ctgggatgat gctttttcta
     6781 agtttgagca gggcgaggca cctataatgc tcagctatgc aacagacggt gcatattctt
     6841 accactacta ccagagtaca aagtataaag ccttcattcc taaggacggc gcatatgttc
     6901 agatagaagg tgccggaatt gtaaaaggct ctaaaaataa gcagctcgca cagaagttta
     6961 ttgatttttt gcttagtgat gagttccagc aggaagtcgc tttaaatcag tggatgttcc
     7021 ctgtaactga tgtaaagctt cctgaagcat ttaaatatgc agttgttccg gaaaaaatac
     7081 tcagcgtcga tataaaggcc ataagctcgc aaatggataa atatataaaa acatgggaag
     7141 agataatgta taaatgaaac tgtcaattaa gaagattccc ggaagtattc ccggaatagt
     7201 ctatgttctg gctgttctta cagcggtttt tcctattttt aaagattttt tcaatttaac
     7261 ttctttttcc gccttgcaag attctcagac catgagaatc ttgtttttca cgttttatca
     7321 ggcgttgctt tcaagcattg cttctctggt tgtgtctatt ataccggcgt attattcgtg
     7381 gcggaaaaaa aatatacttt cagctatgat agatctttca ttttttatcc ccttcttttt
     7441 tccggtagta tctatgacga tagcttttac ggctttatac ggaagatccg gactgttctt
     7501 tgcactcttt ccgggtgtat ctctttctta ctcactcatt ggaattgtcg ttgctcatgt
     7561 gttttatgac tcacccatat ttgtcaagta tatctccgag ggcttgcgtt ctgttcctca
     7621 aagcctttgt gaatcagcac agatggatgg tgcttcaaaa acagtcatat ttttttcaat
     7681 tcagctgcgt cttattcttc ctgctctggt aaaagctttt ttgcttgttt ttacatactg
     7741 ctttatgagc tttggcgtta tcctttcaat tggcggaatg aaattttcta cggttgaggt
     7801 ggccataagt tcgactataa gcagcagctt tgatttttct aaggctatag ttctttgtgc
     7861 cgttcagttt cttgttatct ttcttttaaa ctacacagga tctaaattta catcttttga
     7921 aggctcagta cagacttatc cgtcttcaaa aagaacagct cttttcacag gtgttttttc
     7981 ttttatatat gttctttttg aatactcact tattgttctt tccttggctg gcggtttttt
     8041 taattttcag gcctctaggt ttgacctgtc aggtcttata aatctttttt ccggccccgt
     8101 gaacgctaaa tatggtataa gtgcttcgtt aatcaactct tcttcagttt cgcttataac
     8161 ggccttaata tgtactgtgc ttgcatatat aatgctgcgt gtttcctcta ggttttcgga
     8221 agtgattatt ctttcgtgcc ttggtatctc acctgctttt atatcaatag cccttttata
     8281 tctaaatata tcctggctgg ttccctatcc tctgttactg gttttcggat acgttctact
     8341 caatatgccg attgcctttt cttttatgta ccagcctgta aaagcttttc catctgttat
     8401 tacagaagct gccaaaatgg acggtgcaac ctctgtctca atcttttcaa ggatccattt
     8461 tcctcttatg tacagaattt ttgtatcttc attcttacag atatttgcta ttatcttcgg
     8521 agagttcacc atgatatata caatgcagat tcaaaaaact ttccctatgg taagcataac
     8581 aaattacatt ctttcttcca acaggatgtt caaagaaagc aatgctctca gctcattaag
     8641 catatttata gtgatctctg tttttttctt ttccaaaatt ctggagaaaa aacacagaag
     8701 tttattggaa taatctactt accgggaggg ttttatgaag aaaaagcatt tttttctctg
     8761 gatattttta ctattattgg tcttagctgt cgttttcact ttactgttta ttttacgcag
     8821 acctgaggaa aaggtttctg ttcttcttta ccataggttc aatgacagcc gatatccttc
     8881 aaccagtgtg gatagtgcag aactcgaaaa tcaaatcctt tttcttaagt caagaggtgt
     8941 ttcttttctg agcgaaaatg attttatttc tttgcttgaa ggcaagaaaa agtacggaaa
     9001 ctcagttcta ataagcattg acgatggttt tagaagtatt ttgactgcat atcctgtact
     9061 taagaa
//
LOCUS       DAOSHR010000077         8879 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_6706_length_8879_cov_4.588055, whole genome shotgun sequence.
ACCESSION   DAOSHR010000077 DAOSHR010000000
VERSION     DAOSHR010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 8879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8879
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6706_length_8879_cov_4.588055"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..4064
                     /locus_tag="PLU28_05600"
     CDS             <1..4064
                     /locus_tag="PLU28_05600"
                     /inference="COORDINATES: protein motif:HMM:NF024487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4011 domain-containing protein"
                     /protein_id="HOG34575.1"
                     /translation="KTDSTSYLEFFTDNIKFSSNGFKSRKEFEINFEKTPADNIQLFF
                     KDEKTFYIRSDEKDKKLVLEHILSDNAFSKKSTGISILYLSYGTLKWKNHVFSQKHLF
                     SPVFLMPISVLRKNENQYLLKIHSEQFIFNPFLKDSLVDMLDCSLLDNHAESFFKDMP
                     EKIPIDWEINQRLFLSLFNIERYMNFKELENRKERLLSLSIVKTLYSSDEKSYTTVEK
                     EKIYRPMKILPVSILDKSQQNAVKLYKKNNDNYAVFSSSFSEKNEVTVNIILQALAEQ
                     KNVLYVYSQEQSASQLKKALKERYLDNIYISPEQTSGKNTDTLKKILTSLDSLDISTR
                     KSDTSIAEDDLISQYEKSENILSEYYITLNTFLGKTGLKVSEVFERYLVFTFKRNPTY
                     RIPNVKSMSIENFSENQSILNEFSLYSDIYKIYSDHPLRELCLEHFEDVSSVDSLKPL
                     FTEYLKYLNFIDSYIKKLIPESKNTIDSSYIDSFIEILRLLKELELIDLTIKTLFTKS
                     RETLLTFCEKLKFFCDSYELARKRFTEEIFFSIPETELMLEHYIANKDKQFRNFLPQF
                     SIAKKYFIKNLRAPSDKPNIDVFISDLHRTFSERQELLADSDFTNIVGAYLKNSRPNI
                     AVLKKIVDDLRSILILIDTSKIPFYIVQNYEQVTDSEINYLKTDFSAFKKIIPSVHCP
                     DSLISQKIELTEKIVNNSFMIVQWFEFTKWLEKLTGKELNAFLDYFCSENFDISVISE
                     VYEETFLLSCIENIFINFPVLRNCDPKYFSDISKKYRVYSEQYVGKNLYDFTGKYKNK
                     SIEPEKILELRKLLFSNIQDISSADLKKIINDNFNIIKEIVPLVILPILSVPDYFFMA
                     EEIFDLIIIDDASTIYSEDVLPLFLKGKRFILAGDIGASSPSKASFSVDNFFNIRSFE
                     TDIKNNFSKNILELALESDMPAVKLTRSYNRLYNRLTTLMADNLYNREIVFYPDIPKT
                     TEGLYLSLVEKNDPHIEDKNFNEAMQVINLINEYRKRFPKYTIGVVTLNSQQKDLIYS
                     VLKDIDASEPIAKSILGLIKDDIVSIEDHSVLLNEKKDIIIFSTVVSSSTLFSNIEKL
                     SAYDLLEEKKRFNTIVSCATHLLHLVTSIDSQSFEKLKTFPQCVFFYNFFSAFFTRES
                     SLDFSESSQRKENLFLDFLCIKLSNEGHQCFIRNDSSEKFIQFKNSESIIIEYDFEIA
                     RTCLNGNERECLRNSYFEQNNIKPYRIWLIDWVKDFAKQYKELIKFIETAETHTFVST
                     NRKKRQSFAAIDKEDICSSIQSILELSSLSEKEIKREVIKKFGFSRLTKEMSSLIETY
                     IQELLQKEIICFSKEDNSYSINITDKSQSLQES"
     gene            4087..4563
                     /locus_tag="PLU28_05605"
     CDS             4087..4563
                     /locus_tag="PLU28_05605"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOG34576.1"
                     /translation="MNDESILINLFTQITFPTNAEKEEAWIDSFSPDSLCEYILAVQK
                     NDTGKFIGGIEINNLDLKNRNCTIAVYMHPDYLGKGVGTQAINILLDFLFQRFSLNKI
                     KLNVFDYNERAIKSYKKVGFVEEGVLRQELFTLNKYHDIVMMSILKQEWNRHDAKN"
     gene            4547..5134
                     /locus_tag="PLU28_05610"
     CDS             4547..5134
                     /locus_tag="PLU28_05610"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOG34577.1"
                     /translation="MTQRTNKTTENKFRKLTELSKTELLKTGIRKFNFEKVISSCGLS
                     KATVYKKFKNKKEFIRHVIKQLFIDLTGPFEDYISDLNSLDSIIDKILNAAANLDKIM
                     IKYHFADVLNSEEITSLINDLYYDTFGKVILKKISQLQQIHEIRSDIDGDIILEFVTS
                     VTKGLSKLMFVKSFEMTGLSYRKLLISALKDGSND"
     gene            5127..6275
                     /locus_tag="PLU28_05615"
     CDS             5127..6275
                     /locus_tag="PLU28_05615"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HOG34578.1"
                     /translation="MIDYIVLCAVGIEKVLCNEIKHLGYKIERTLPGKIYVKADEAFI
                     YTANINLRTAERIMMVLKTFQAITFDSLYENIKSIRWSDYIPKNTNVEITKLSSVKSQ
                     LYALSSIQSVSHKAVYDSLKLSYNQTEHLSKDTQAVEIRIYLNNDIAEVCLDLSGEPL
                     HKRGYRKLVSQAPLKETIASAMLLTSGWKRKFPLYDPFCGSGTIPIEAALYAMNIAPG
                     LYRSFSVQNLKFYSQDQFIKAKQLAVSKIREYDASEFSICASDKDKEIISCAIKNADN
                     FKLAQYITFMTESMEKLIDRSGIGYVVTNPPYGKRIRDEKYATKVYEQMRYLKKVFKS
                     WTYVIITDSDDFESYFDMKASKKLTIHNGSIVNTVYTFDNASEKSVIS"
     gene            6292..7530
                     /locus_tag="PLU28_05620"
     CDS             6292..7530
                     /locus_tag="PLU28_05620"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HOG34579.1"
                     /translation="MKKVYIQFIALILFLCVSAYASQASPEKNGFFFFKDDVQDTYQK
                     DLKDFIVDFPGICGCEILSEDKSVFFSLESSKLFIPASLNKLITAFSALETFGPEYRY
                     PTKISSDSKPTPVFGGNIYIKGFGNPVLNPQKYKDVLSYIVKTYAIKELNGDIVIDFS
                     YFDQSQYYGKGWMWDDPQPQISALNLWLENYSIFMNKPYSLIEDMIGYYTLASLKDFG
                     VVFNGKIKNGKMPENKTLLYTHQSEELFDIVKIMLEKSDNYIAEHIFRSIGANIYGLG
                     STENSVKVYENLLFSKLGLLKKNYIIADGSGLSMYNLLSPQLLNSLNYFMYTKYKEKY
                     LDILATPGEKSTIENRFKFSTLWAKTGTLYSDSGISGIIKTKKNNLYFFTLMENNYTS
                     SNLEIKEFETKFLTLLYENY"
     gene            complement(7558..8775)
                     /locus_tag="PLU28_05625"
     CDS             complement(7558..8775)
                     /locus_tag="PLU28_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOG34580.1"
                     /translation="MIKNAVYAQSGGVTSVINASAYGVIKKCFSCGEIGKVYVGLNGI
                     NGIIGDDLADMDCEEPYEIDLLKTTPASAFGSCRRKLSESHEDEFKKIFEVFSKYNIG
                     YFFYNGGNDSMDTANKIYNYSKKIGFDLSVIGIPKTIDNDLYGTDHSPGYGSSAKYLA
                     TAILEASLDVKSMSADSTKVFILETMGRHAGWLAASASLARVNANYGPHIVLIPEVPF
                     NREKFITKVDNTVRRTGYCSIVVAEGLNDDKGNLVAHFGATDSFGNKQLGGVGSTISS
                     IISEELKLKVHTAVPDYLQRSGGHLSCYTDVNEAVLVGSMAVHYALQGKNGYMVSINR
                     ISDEPYYVTYDLVDLSEVANNTKTIPNEFITDDGMDVTTEFIKYAKPLIEGEYYPPIL
                     NGTPRYAKLRLIR"
BASE COUNT         3080 a         1443 c         1431 g         2925 t
ORIGIN      
        1 ttaaaacgga tagcacatcc tatctagagt tttttacaga taacataaaa ttttcttcaa
       61 atggttttaa atcacggaaa gagtttgaaa tcaactttga aaaaactcca gcggataaca
      121 ttcaactgtt tttcaaggat gaaaagactt tttatatccg ttctgacgaa aaagacaaaa
      181 aattagtatt ggagcatatt ttatctgata atgctttttc aaagaaaagc acaggaatta
      241 gcatcctata tctatcgtat ggtacattaa aatggaaaaa tcatgttttt tctcaaaaac
      301 atttgttttc accagtattt cttatgccta tttcagtttt aagaaaaaat gaaaatcagt
      361 atcttcttaa aattcatagt gagcaattta tttttaatcc ttttcttaag gattcgcttg
      421 tcgatatgtt agactgttct ttacttgata atcatgctga aagttttttc aaggatatgc
      481 ccgaaaaaat accgattgac tgggaaataa accaacgttt atttttatct cttttcaaca
      541 ttgaaagata catgaacttc aaagagcttg aaaacagaaa agaacgactt ctgtctttat
      601 ctatcgtaaa gacactttat tcgtcagatg aaaaatcata tacgactgta gaaaaagaaa
      661 aaatctacag acctatgaaa attttacctg taagcatact tgacaaatca cagcaaaatg
      721 cagtaaagct ttataaaaaa aacaatgata attatgctgt tttttcttca tcattttcag
      781 aaaaaaatga agtgacagtt aatataatac ttcaggcatt ggcagagcaa aaaaatgttt
      841 tgtacgttta ttctcaggag cagtctgctt ctcaactcaa aaaagcatta aaggaacgat
      901 atttagataa tatctatata tctcccgagc aaaccagtgg taaaaatacc gatactttaa
      961 aaaagatact tacctctttg gacagcttgg atatatctac tcgcaaatct gatacttcga
     1021 ttgcagaaga cgatctgatt tctcagtatg agaaatcaga aaatattctt tcagaatact
     1081 acattactct gaatactttt ctgggtaaga caggtcttaa agtttcggaa gtatttgaaa
     1141 gatacttagt ttttactttc aaacgaaatc caacgtaccg tattccaaac gttaagagta
     1201 tgtccattga aaattttagt gaaaatcaaa gtattttaaa tgaattttcc ttatacagcg
     1261 atatatataa aatatacagt gatcatccgt taagagaact ttgccttgaa cattttgagg
     1321 atgtttcatc agttgattcc ttgaagcctt tgtttacaga gtacttaaaa taccttaatt
     1381 tcattgattc atacataaaa aaactgatac ccgaaagtaa gaatacaata gattcttctt
     1441 atattgatag tttcattgaa attctaaggt tattaaaaga actggaatta atagatctta
     1501 ctataaagac actttttacc aaaagccgtg aaacgcttct tactttctgc gaaaaactta
     1561 aatttttctg cgactcttat gagcttgcaa gaaaaagatt cacagaagaa atttttttca
     1621 gtatccctga aactgagctt atgcttgaac attatattgc caacaaagat aagcagttta
     1681 gaaatttttt accacaattt tctatagcca agaagtattt tattaaaaat cttagagctc
     1741 catctgataa accaaatatt gatgttttta taagcgatct gcacagaact ttttctgaaa
     1801 gacaggagct gcttgcagat tcagatttta caaatattgt aggagcatac ctaaaaaatt
     1861 cccgtcctaa tattgctgtt cttaaaaaaa ttgtggatga cctgcgaagc attctcattc
     1921 ttatagatac atctaaaata cctttttata ttgtacagaa ctatgagcag gttactgatt
     1981 cagagataaa ttatctcaag acagattttt ctgcttttaa aaagattatt ccttcagtac
     2041 attgcccgga ctctcttata agccaaaaga tagaactaac tgaaaaaata gttaacaatt
     2101 cttttatgat agtacaatgg ttcgaattta caaaatggct ggaaaaactt accggaaaag
     2161 aactaaatgc ttttctggat tatttttgtt cagaaaattt tgatatttcg gttatatcag
     2221 aggtatatga agaaaccttc cttttgtcat gcatcgagaa tatttttatt aattttccgg
     2281 tactacgaaa ctgtgaccca aaatactttt ctgatatctc taaaaaatat agagtatact
     2341 ctgaacagta cgtgggaaaa aatctttatg atttcactgg aaaatataaa aataagagta
     2401 tagagcctga aaaaatactt gaactgagaa aactgctttt ctccaatatc caagacatca
     2461 gctcagctga tcttaaaaaa attattaatg ataattttaa tattataaaa gagatagtac
     2521 ctcttgtcat tcttcctatt ctttctgtac cggattattt tttcatggct gaagaaattt
     2581 ttgacctgat aataatagat gatgcttcaa ccatctattc ggaggatgtt ttgcccttgt
     2641 ttctaaaagg taagaggttt atacttgctg gcgatattgg tgcttcttca ccttccaaag
     2701 catcattttc tgtagataat tttttcaaca tacgttcctt tgaaacagat ataaaaaata
     2761 attttagcaa aaatatactc gaacttgctc ttgaatcgga tatgcctgct gtaaagctta
     2821 cacgaagtta taatcgtctt tacaacagac ttaccacact gatggcagac aatctttata
     2881 atcgtgaaat tgttttctat ccagatatac ccaaaactac cgaaggactg taccttagtt
     2941 tggtggaaaa aaacgatcca catattgaag ataaaaattt caatgaagcc atgcaagtaa
     3001 tcaatcttat aaatgagtac agaaagaggt tcccgaaata tactatagga gtagttacac
     3061 ttaattccca gcagaaagat cttatttatt ctgttctaaa agacatagat gccagtgaac
     3121 ctattgcaaa aagtatttta gggctcataa aagatgatat tgtttcaatt gaagatcatt
     3181 cggttttgct caacgaaaaa aaagatatca ttattttttc taccgtggta tcttcctcaa
     3241 cacttttttc caatatagaa aagctttctg catatgatct gctcgaagag aaaaaacgtt
     3301 ttaatacaat tgtttcttgt gcgacacatt tgttacatct tgtgacttcg atagatagcc
     3361 agagctttga aaaattaaag acttttcctc agtgtgtatt tttttataat ttcttttcag
     3421 ccttttttac aagagaaagc tcgctagact tttcagagtc ttcacaacgc aaagaaaacc
     3481 tttttcttga ttttttatgt ataaagcttt caaacgaagg tcatcagtgt tttatacgta
     3541 atgactcatc tgaaaagttt attcagttta aaaactctga atcaataatt attgagtatg
     3601 attttgaaat tgcaagaaca tgcttgaatg gaaatgaacg tgaatgcctt agaaacagtt
     3661 attttgaaca aaacaacata aaaccttacc gtatatggct tatagactgg gtaaaagatt
     3721 tcgcaaagca gtataaggaa ctcataaagt ttattgaaac agcagaaact catacctttg
     3781 tttcgactaa cagaaaaaaa cgtcaatctt tcgctgctat cgataaagaa gatatatgtt
     3841 cttctattca aagcatactt gaactttctt ctctttcaga aaaagagata aaacgtgaag
     3901 taataaaaaa atttggattt tcaagattaa caaaagaaat gtcaagcctc atagaaactt
     3961 atattcagga acttcttcag aaggaaataa tttgtttcag taaggaggat aacagttatt
     4021 caatcaacat tacagataag tctcagagct tacaagaaag ctgacgttat taactttaat
     4081 gaggtcatga atgatgaaag tatactcata aatcttttta cgcaaattac ttttcctaca
     4141 aatgctgaga aagaagaagc atggattgac tctttttcac ctgattcttt gtgtgaatat
     4201 attctggctg ttcagaaaaa tgatactggt aagtttatag gtggtataga aataaataac
     4261 ttggatctga aaaacagaaa ctgtacgata gctgtctata tgcatccaga ttatcttgga
     4321 aagggagttg gtacacaagc catcaatatt cttttagact ttctttttca aagattttct
     4381 cttaacaaga taaagcttaa tgtttttgat tataatgaaa gagctataaa aagctataaa
     4441 aaagttggtt ttgttgaaga aggcgtatta agacaggaac tgtttacttt gaataagtat
     4501 cacgatattg ttatgatgtc cattttgaaa caggagtgga accgacatga cgcaaagaac
     4561 taacaaaacc actgaaaata agtttagaaa attgactgaa ctttccaaaa cagagcttct
     4621 taaaacagga atacgtaagt ttaactttga aaaagtaata agttcttgcg gattaagcaa
     4681 agcaacagta tataaaaagt tcaaaaacaa aaaagaattt atcagacatg tcattaagca
     4741 gttgttcatt gatcttacgg gaccgtttga agattatatt tccgatctga actcacttga
     4801 ctcgataata gataaaattt tgaatgcagc agcaaatctt gataaaataa tgataaagta
     4861 tcactttgct gacgttttaa attctgaaga aattacctct ctgataaatg atctttacta
     4921 tgacaccttt ggaaaggtaa ttctgaaaaa aatttcccag ctgcagcaaa ttcatgaaat
     4981 aagaagcgat atagatggag atataattct tgagttcgtt acttccgtta caaaaggttt
     5041 aagtaagttg atgttcgtaa aaagctttga aatgacaggt cttagttaca gaaaattgct
     5101 gataagcgct ttaaaggatg gttcaaatga ttgactatat tgttttatgt gcagtaggga
     5161 tagaaaaagt actatgcaac gaaataaagc atcttggcta taaaatagaa agaactcttc
     5221 ccggaaaaat ttacgtaaag gctgatgagg cttttatcta tacagccaat atcaatctga
     5281 gaactgcaga aagaattatg atggtcttaa aaacttttca agccataact tttgacagtc
     5341 tttatgaaaa tataaagagc atcagatggt ctgactacat acctaaaaat acaaatgtcg
     5401 aaattaccaa attaagttct gtaaaatcac agttgtatgc attgagttct atacagtctg
     5461 tttcacataa ggctgtttat gacagtttaa agctttcgta caatcaaaca gagcacttat
     5521 ccaaagatac acaggctgta gagataagaa tttatctcaa taacgatatc gcagaggtat
     5581 gtttggatct ttcaggtgag cctcttcaca aaagaggcta cagaaaactt gtaagtcagg
     5641 ctcctcttaa agagactata gctagcgcaa tgctgcttac gagcggatgg aagagaaagt
     5701 ttccgttata tgatcctttc tgtggttcag gaaccatacc aatagaggct gctctgtatg
     5761 ctatgaatat agcacctggt ctgtacagaa gtttttctgt gcaaaatctt aaattttaca
     5821 gtcaggatca atttataaaa gcaaagcagc ttgctgtttc taaaatacga gagtatgacg
     5881 cttctgagtt ttcaatatgt gcaagtgaca aggacaagga aattattagc tgtgcaataa
     5941 aaaatgcaga taattttaaa cttgctcagt atatcacctt tatgactgag agtatggaaa
     6001 aacttattga tcgttcaggt attggatatg tggttactaa cccgccatat ggaaaaagaa
     6061 tacgcgacga aaagtatgcc acaaaagtat atgaacagat gcgttatctg aaaaaagtct
     6121 ttaagtcttg gacgtacgtt ataattaccg acagtgatga ttttgaaagc tactttgata
     6181 tgaaagcatc taaaaagctt actattcata acggtagtat tgtgaataca gtttatactt
     6241 ttgataatgc ttctgaaaaa tctgtaatct cttaaaacgg cggtggtgtt tttgaaaaaa
     6301 gtatatatac agtttatagc cttaattcta tttttatgcg tttcagccta tgcttctcag
     6361 gcatctcctg aaaaaaatgg ttttttcttt tttaaagacg acgttcagga tacttatcaa
     6421 aaagatctta aagattttat tgttgatttt ccgggaattt gcggatgtga aatactctca
     6481 gaggataaga gtgttttttt ctcccttgaa agtagtaagc ttttcattcc agcttcacta
     6541 aataaactga taacagcatt ttctgcactt gaaaccttcg gtccggagta cagatatcct
     6601 acaaaaattt cttctgattc aaaaccaaca cctgttttcg gaggaaacat ttacataaaa
     6661 ggctttggaa atcctgtact taacccgcaa aaatataaag acgtactttc ttatattgta
     6721 aaaacttacg ctattaagga gcttaacggc gatatagtaa ttgatttttc gtattttgat
     6781 caatcacagt actatggaaa gggatggatg tgggatgatc cacagcctca gatttccgct
     6841 ttaaatctct ggcttgaaaa ttattctatc tttatgaaca agccatactc tttaatagaa
     6901 gatatgattg gttattatac tcttgcatcg ttgaaagatt tcggtgtggt tttcaatgga
     6961 aagataaaaa atggtaaaat gcctgaaaac aaaactcttc tttatactca tcaatccgaa
     7021 gagctttttg atatagtaaa aattatgctt gaaaaaagcg ataactatat tgcagaacac
     7081 atcttcagaa gtattggcgc gaatatttac gggcttggaa gtaccgaaaa ttctgttaaa
     7141 gtttatgaaa atctactttt ttccaaactt ggattactca aaaaaaatta tattattgct
     7201 gatggttctg gactgtctat gtacaacctt ctttctccgc agcttctgaa ctcgcttaac
     7261 tattttatgt acactaagta taaggaaaaa tatcttgata ttttggcaac tcccggagaa
     7321 aaaagcacta tagaaaatag attcaaattc agtactttat gggcaaaaac cggtacttta
     7381 tactctgatt cagggataag cggcattata aaaactaaaa aaaataatct gtattttttc
     7441 acacttatgg aaaataatta tacttcttct aacttggaaa taaaagaatt tgaaacaaag
     7501 tttctaactc ttttgtacga aaattattaa aaaagaagcc tcattagagg cttcttttta
     7561 tcttataagt ctaagtttag catatcttgg tgtaccgttc aatataggag gatagtactc
     7621 tccttcgata agaggcttgg catactttat aaactctgtt gtaacatcca ttccgtcatc
     7681 tgtgataaat tcattaggaa tagttttggt gttatttgcg acttctgaaa gatccacaag
     7741 atcatatgta acataatatg gttcatcgct tatacggttt atacttacca tatatccgtt
     7801 tttcccttgt aaagcataat gtacagccat gcttcctaca agaactgctt cattaacatc
     7861 tgtatagcat gaaagatgac ctccgcttct ctgtagataa tcaggaacag ctgtatgtac
     7921 ttttagttta agttcttctg aaattattga ggatattgta ctgcctactc cccctagttg
     7981 tttgtttccg aaactgtctg ttgctccgaa atgggcaaca agattacctt tatcatcgtt
     8041 aagaccctct gctacaacta ttgaacagta acctgtcctt cttacggtat tatctacttt
     8101 ggtaataaac ttttctctgt taaatggaac ttcgggtata agaactatat gaggtccgta
     8161 gtttgcattt actcttgcaa ggctcgctga agcggcaagc cagcctgcat gtctgcccat
     8221 tgtttcaaga ataaaaactt ttgttgaatc agcagacatg ctttttacat ccagtgaagc
     8281 ttcaagaata gctgttgcaa gatacttggc cgaagatccg taccctggag aatgatcagt
     8341 accgtaaaga tcgttatcga ttgtttttgg aatacctatt acactaagat caaatcctat
     8401 tttttttgag taattataaa ttttatttgc agtatccatg gaatcgttac caccattata
     8461 gaaaaaatat cctatattgt actttgaaaa aacctcaaat atctttttaa attcatcttc
     8521 atgagattcg gaaagcttac gcctgcacga accaaaagca ctagcaggag tggtttttaa
     8581 aagatcaatt tcataaggct cttcacagtc catgtcagca agatcatctc caattatacc
     8641 gtttatgccg ttaagaccta cataaacctt accaatttct ccacaggaaa aacacttttt
     8701 tataactccg tatgctgaag cattaataac acttgttaca ccgccagatt gagcgtagac
     8761 tgcattcttt atcatactta tctactcctt tatgatatta ggtaaacagt ttgcatgtaa
     8821 ttatctttaa ataactgtat ttgataaaaa ctctttttcg tacttatctt cagattttg
//
LOCUS       DAOSHR010000078         8837 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_6750_length_8837_cov_5.829879, whole genome shotgun sequence.
ACCESSION   DAOSHR010000078 DAOSHR010000000
VERSION     DAOSHR010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 8837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8837
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6750_length_8837_cov_5.829879"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="PLU28_05630"
     CDS             complement(<1..346)
                     /locus_tag="PLU28_05630"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF012470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HOG34581.1"
                     /translation="MKKFLIAIVVLICSVWVSAAPKNLIVIMGDGMGMNHLFFTELLS
                     QGKSLPVYTEQFEVRAVGTNTPMDTLITDSAAAATAFFCGVRTNTSFLGLDPQEKEYD
                     SLMKIFKTKGYTT"
     gene            complement(360..1202)
                     /locus_tag="PLU28_05635"
     CDS             complement(360..1202)
                     /locus_tag="PLU28_05635"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOG34582.1"
                     /translation="MAVRSKKVNDTVFYTFCYVILIIFAIVFLFPFIWMILSTFKTQS
                     ELMAFPPRFLPRSFSLANYKEIFDTVPFGKFYINSLIVTTCAVFLQLLTSSMAGFAFA
                     KYDFRGKNGIFKTLVSAMMIPFPVTIIPLYIMVYSLGLVDTYAAMIVSGCVSIFGTFL
                     MRQFIMGIPNDLLEAARIDGCGEWMIYTKIILPNIKPVLSALGIFGFMSSWNAFLWPL
                     LVVNDNRHRTVQLGVQFFSQQYGDLVHLKITAAAMAVIPIVIIYLILQKQFIQGITMT
                     GMKE"
     gene            complement(1205..2446)
                     /locus_tag="PLU28_05640"
     CDS             complement(1205..2446)
                     /locus_tag="PLU28_05640"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOG34583.1"
                     /translation="MELTKKSQLIAIFGFIIIVAGFFFPVTMGERPIFIWEFSFLFNA
                     NMAIVFGVFAILTFVFAMMRKTNWLLSAAICDLILVLSFVFMLGTWKQSVPSKFYLHF
                     FRAASIGWYLLLAGVVLVLIVTLRSLPKKKVAPYLFILPMLLGIGFLTFFPAIFAVYV
                     SFRKWNIMVPKKPFVGFDNYIKAFQDKYFWDSLLISFKYAIVVIPSKMILAYFFALLI
                     YAIPKFKSFFRVVYFLPTVTSVIAVSVIWSWIYHPSFGLANYILEMFRLPTVDWLGEP
                     NIAIFSVAIVSIWKGIGYDVIIFLAGLNAISESVIEASEIDGATRWQKLKYIITPLMR
                     PSLVLIFITSTIGAIQVFSEIYMMTGGNADTKTAVFYIWEYGFTRLNMGYASAMSMIF
                     FGIILIITLIQMRVTKLLKEE"
     gene            complement(2495..3718)
                     /locus_tag="PLU28_05645"
     CDS             complement(2495..3718)
                     /locus_tag="PLU28_05645"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOG34584.1"
                     /translation="MKRYALLILVVMLVSVFFAKTTVVHWMHHSPARAEIVGNLADEF
                     MKANPDIEIVVQTIPEGEYKTKLLAAVAAGSGPDVAQVPARSMTELLDYGIISEFPSK
                     VVTTQYMKDYFMPATIESLIINNKLYGIPTDVQTIMLFYNTNLFKAAGIKEPPKDWNE
                     LREDAIKLAKWDGDKMIQAGLGIEGYHPVIETFMVMNGATFTDPKNSNRIVVEDAQVQ
                     AMKFLTDLVTKDKVYTRKFGARYTMFRQNVQAMVFGHGAMLGSLQVGAVPELEFKVAP
                     VPKNPVTGEQKTVLTSWALVIMDSCRNKEAAAKWLMYVSSENAQKQWIKTGELPSLKS
                     VINDKEIRKDERLAVILDSLNYAVPTKSKGWADPFSKFRDIGFNEVINKGADPKKVVE
                     NAVLEINKYLAETFN"
     gene            complement(3879..5024)
                     /locus_tag="PLU28_05650"
     CDS             complement(3879..5024)
                     /locus_tag="PLU28_05650"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HOG34585.1"
                     /translation="MLTNLTETEQKVFQLIWYKKYGISRTEIVNKTKLSKATVSRAVD
                     KLISNGFVVEGDAVPTTSKGRPQKLLVLRNDEILISGISIHSRFLHFVIGDLSGRLRY
                     SENIPVDKSNVVSTIMDFPKYLEKNYNSLYKNIKVFSIVTPGQVDQVNMKVTGNFFSV
                     EKDVYFSGLEDCWNILFKVENDVNALLSSQIMFGHLKYKNIFLIDSEFGGALVIDGKM
                     VRGLNGGAGEFGHTQLIHDGPLCWCGKKGCVSNYFGADNLKSIFHSTIPASYMALFGR
                     DFSVMLSELYNEKFSKEFCILYEKQLDLLADAIVDFLDLLCIDNLMLNTENPFFKDRS
                     FEYISEKMRKTSGLKDKLKVMPLKIRKGIEIPLAISLTKFLGENIEI"
     gene            5358..7094
                     /locus_tag="PLU28_05655"
     CDS             5358..7094
                     /locus_tag="PLU28_05655"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOG34586.1"
                     /translation="MKKIITVLIMLIFSALSVFSYGLSICGGGAYAFKCIGFLQALSE
                     EGFCPDEVASTSLGSIAGYFMACGYDMSHVHKLFSKINFTDLFDISLPLNGGFLNSAR
                     LGDFIAFSSGCDSYLDMQSALKMAALNMNTLNVEFLDKGDPIKALRASTAMQGHFEPV
                     VINSDFFADAGVKELSIINPQEIFCSDENFLIRLTPSYRDTSDIDFRNIINVIFAAIE
                     AGGINRDLYAADKLSYNEIFDIESSIPIDVKFFSKGLVFYNDGYLAGKKFLNENPDFF
                     ERNNIPQKNIQKKTLDIESICTSLRTESYLRPREFYWTVKADIHQENSDKFVRSYIYS
                     EFLNSRIRAGIAYKFNDTLYPYISLQTYYFPLRCLRFTAEYVHNMRYGITLRTYLMEK
                     YKSIVYADLSYTHGIKDDNLFTFGLTFDGMYSYNSANLGPELSLKILSDLNIINGMLS
                     IKNIYGDDYMNLSVSSSYNYKNNFNQIPDILCKYPFIHKISQQYALKIPLIKNLDIDM
                     FQTFLLQDIYGKFYFNWAIDDFILSSFLPSCGLSLFFPFRFAGVSNSFIECGINYRDS
                     FSFFFSLSDYLF"
     gene            7158..7502
                     /locus_tag="PLU28_05660"
     CDS             7158..7502
                     /locus_tag="PLU28_05660"
                     /inference="COORDINATES: protein motif:HMM:NF024087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HOG34587.1"
                     /translation="MEKIIACCGLTCDTCDAFKATSMNDDTLREETAKKWGGMYGAQF
                     KPKDINCLGCHSNTLFGYCNNCDIRACSKSKGFNNCGECTDYVSCEKIAKFTEMVPSV
                     KPLLDQIHNSTR"
     gene            7621..8151
                     /locus_tag="PLU28_05665"
     CDS             7621..8151
                     /locus_tag="PLU28_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34588.1"
                     /translation="MMQLNNEYEYISQTKVFGIPLIHIKGHGTAKGIVAIGPLALGII
                     SIGILPAGLISLGALSTGILLAIGGLSLSLFTAIGGVALSFYLSIGGVGISYFYSLGG
                     AAIANHCAIGGAAGAKLAVGASAKGNLAIFSQKGYGQTVIDYTKVSAEYVLQSIKELY
                     PYISGWFIELVKLTIR"
     gene            complement(8162..>8837)
                     /locus_tag="PLU28_05670"
     CDS             complement(8162..>8837)
                     /locus_tag="PLU28_05670"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG34589.1"
                     /translation="MDPNKKEYYESRGSAYLALKQYEPAIADYTKAIALDPNYTHAYN
                     GRGTAYISLGKSIPAIFDYTKAISLDPNYASAYNGRGSAYLALKKNIPAIFDYTKAIT
                     LDPNYVPAYSVRGHTYLKLSEYKSAITDYSTAIKMDPNNALVYNNRGYAYLCLKQYKS
                     AITDFSTAIKLDPSIKECYANRGHAYLDLEQYYLAKKDFKKFLSLVPEDDQTAVQIKK
                     FLNSLE"
BASE COUNT         2736 a         1748 c         1601 g         2752 t
ORIGIN      
        1 ccgttgtata tcccttggtt ttaaagatct tcataagtga gtcgtactct ttttcctgtg
       61 gatcaagtcc gagaaagctt gtatttgttc gtacaccgca gaaaaaggct gttgctgcgg
      121 ctgcagaatc tgttataagg gtatccatcg gagtatttgt ccctacagct cttacttcaa
      181 actgttctgt gtatacgggc aaactttttc cctgtgaaag aagctcggtg aaaaaaagat
      241 gattcatccc cataccgtca cccattataa ctatgagatt ttttggagcg gcagataccc
      301 agactgaaca gattaaaact acaattgcta tcaagaactt tttcattttt atctccttcc
      361 tattctttca ttcctgtcat ggtaattcct tgtataaact gtttctgaag tataagatag
      421 attattacta ttggaattac agccattgca gctgcagtta ttttaagatg aacaagatca
      481 ccgtactgct gggagaagaa ctgcacaccc aactgaactg tcctgtgcct gttgtcattt
      541 accaccagta aaggccatag aaatgcgttc cacgaggaca tgaacccaaa tattcccaac
      601 gccgaaagaa caggcttgat attgggaaga attatcttgg tatatatcat ccattctccg
      661 cagccgtcta ttcttgcagc ttcaagaaga tcattaggta ttcccattat gaactgtctc
      721 ataaggaacg taccaaatat gcttacacag cctgaaacta tcatagcagc atatgtatcg
      781 accaatccta atgagtatac catgatgtaa agcggaatta ttgtaacagg aaaaggtatc
      841 atcatggcac ttacaagtgt tttaaatata ccgttctttc ctctgaaatc gtacttggca
      901 aaggcaaaac ccgccatgct tgatgtcagc agctgaagaa aaacagcaca tgtggtgaca
      961 attaaactgt ttatataaaa tttgccgaaa ggaaccgtat caaatatctc tttatagttg
     1021 gccagagaga aggatctcgg caaaaaccta ggcggaaatg ccatgagctc gctctgagtt
     1081 ttgaaggttg aaagaatcat ccatataaaa ggaaaaagaa atactatggc aaatattata
     1141 agtatcacat aacaaaaggt ataaaaaact gtatcgttta ctttttttga tctgaccgcc
     1201 attttcattc ctccttgagc agtttcgtaa ctctcatctg tataagagtt attatgagaa
     1261 ttataccgaa gaatatcata gacatggcgc tcgcatatcc catgttaagg cgtgtaaatc
     1321 catattccca gatgtagaag actgcagttt tggtatcagc atttcctcca gtcatcatgt
     1381 atatttctga gaaaacctgt atggctccta ttgttgaggt tataaatata agcaccagag
     1441 aaggccgcat aagaggtgtt attatgtact tcagcttctg ccaccttgtt gccccatcta
     1501 tttctgaagc ttcaattaca gattctgaaa ttgcgttaag tcccgcaagg aatattatta
     1561 catcataccc gatccctttc catatggata cgattgcaac tgaaaatatg gctatattag
     1621 gttctcccag ccagtccacc gtcgggagcc tgaacatctc aagaatatag ttggcaagac
     1681 cgaatgaagg atgatatatc cagctccata taacgcttac cgctataaca gaggttaccg
     1741 taggcaaaaa gtagaccacc ctgaaaaatg atttgaactt gggtatcgca taaataagca
     1801 gggcaaaaaa atatgcaagg atcatttttg aaggaataac aactatggcg tacttgaagc
     1861 ttattaatag cgaatcccaa aaatacttgt cctgaaaagc ttttatatag ttatcaaagc
     1921 cgacaaaagg ctttttcggc accattatat tccactttct aaaactcaca tataccgcaa
     1981 atattgcagg aaaaaaagtc aaaaatccta ttcctaaaag catgggcagg ataaaaagat
     2041 atggagcaac cttcttcttc ggaagcgatc ttaaagttac tatcaacact aaaaccactc
     2101 cggcaagcag caagtaccag ccgatagaag ctgctctgaa aaaatgcagg taaaatttgc
     2161 taggaacaga ttgcttccaa gttcctaaca taaaaacaaa tgaaagaacc agtataagat
     2221 cacatattgc tgccgacaaa agccagtttg tcttgcgcat catcgcaaaa acaaatgtca
     2281 gaatagcaaa tactccaaaa acgattgcca tattggcatt aaaaaggaag ctgaactccc
     2341 atatgaatat cggtctttct cccattgtga cgggaaagaa aaaacccgca acaataatta
     2401 taaacccaaa aattgctata agctgcgatt ttttagtcag ttccatagtc atcctccaaa
     2461 aggtctcccc tttcggggag actcaataac ttaattagtt aaatgtttca gcaagatact
     2521 tgtttatttc aagaacggca ttttcaacaa cttttttggg atctgcacct ttattaatta
     2581 cttcattgaa gcctatatcc ctaaactttg aaaaaggatc ggcccagcct tttgatttgg
     2641 taggtacagc atagttcagt gaatcaagaa taacagcaag cctttcgtct tttctgatct
     2701 ctttatcatt tataaccgat ttaagtgatg gaagttcacc ggttttaatc cactgtttct
     2761 gagcattttc agaagaaaca tacataagcc atttggctgc ggcttctttg tttctgcagc
     2821 tgtccattat aaccagtgcc cagctagtaa gaactgtttt ctgttctcct gttacgggat
     2881 tcttcggtac cggagctact ttaaattcaa gctcgggaac tgctccaacc tgtaaagaac
     2941 caagcattgc accatgaccg aaaaccattg cctgaacatt ctgcctgaac attgtatatc
     3001 ttgctccaaa ctttctggtg tataccttat cctttgtcac aagatcggta aggaacttca
     3061 tagcctgaac ctgtgcatcc tcaactacta ttctgttact attttttgga tcggtgaagg
     3121 tagcgccgtt cataaccata aaggtttcaa tgacaggatg atatccttct attcccaagc
     3181 ccgcctgaat catcttgtcg ccatcccatt ttgcaagttt aatggcgtcc tctctgagct
     3241 cattccaatc ctttggaggt tcttttatgc ctgctgcctt gaaaaggttt gtgttgtaga
     3301 aaagcattat agtctggaca tctgtaggaa taccgtaaag tttattgttg atgataagac
     3361 tctcgattgt ggcaggcata aagtagtcct tcatgtactg tgttgtaaca actttggaag
     3421 gaaattccga tattatgcca tagtcaagca gttctgtcat tgatctggca ggaacctgtg
     3481 ccacatcagg gcctgaacct gctgcaactg ctgcaagaag tttggttttg tactcgcctt
     3541 cgggtatggt ctgaactact atttcgatat ccggattggc tttcatgaat tcatcggcaa
     3601 gattcccgac aatttcggct ctggcaggag aatggtgcat ccaatgaacc accgtcgtct
     3661 ttgcaaagaa aacacttaca agcataacca ctaaaatcag taaagcataa cgcttcatct
     3721 tttcttacct cctttataaa gttagaataa aacccttgaa tatacttaat acttaaaata
     3781 aaatttcagc gtaacctttc ctttttcact gaaaaaatca tatctgtagt caagattagc
     3841 cgttacatca cttatctttc cgcttatcca taatgccttt aaatctcaat attttcacct
     3901 aaaaatttgg taagcgatat ggcaagaggt atttcaatac ctttccttat tttaagcggc
     3961 ataactttaa gcttatcctt caatccggat gtttttctca ttttttctga aatatactcg
     4021 aatgatctgt ctttaaaaaa aggattctcg gtatttaaca taagattatc tatacataga
     4081 agatccaaaa agtcaacgat ggcatcggca agcaggtcaa gctgcttttc ataaagaata
     4141 cagaactctt tggaaaattt ttcattgtac agctcagaaa gcataactga aaagtctctt
     4201 ccgaaaagtg ccatataact tgccggtatg gtactgtgaa agatggattt aagattatcg
     4261 gccccgaagt aatttgaaac acagcctttc ttgccacacc aacatagcgg accgtcatgt
     4321 attagctgcg tatgcccgaa ctcgcccgct cctccgttaa gacctctaac catctttcca
     4381 tctattacaa gtgctcctcc gaactcagag tcaataagga aaatattctt atacttgagg
     4441 tgaccgaaca ttatctgcga agaaagaagt gcattcacat cgttttctac tttaaaaagg
     4501 atgttccagc agtcctcaag tcctgaaaaa taaacatctt tttcaacaga gaaaaaattc
     4561 cccgtaacct tcatattgac ctgatcaacc tgtccgggag taactatact gaatactttg
     4621 atatttttgt aaagactgtt gtagtttttt tccagatact ttggaaagtc cattatggta
     4681 gaaaccacat tactcttgtc caccggtata ttttcagagt atctcagtct tccggaaaga
     4741 tctcctatga caaaatgtaa aaacctgctg tgtatagata ttccgcttat gagaatttca
     4801 tcgttgcgca aaaccagcaa cttctgaggc cgtccttttg aagtggtagg aacggcatct
     4861 ccttctacca caaaaccatt ggaaatcagc ttgtcaactg ctcgcgaaac agttgcctta
     4921 ctgagtttgg ttttgtttac aatctcagta cgtgatatcc cgtacttttt ataccatata
     4981 agctgaaaga ctttctgctc tgtttctgta agattagtca acataatcac ctttattttt
     5041 gtttccatac gaaattatat tttttaaaga tagcttctat gttatctttt gttaatatat
     5101 aaaccttttt tccaaggatg aaactaatat aagtttatca tcgtttacaa ccagaaaaaa
     5161 gcccttttta gaggatgtat aaaaggcagg actgataaac ggctaaaaaa tataattttt
     5221 tataaaatag attatcctgt attttgtttc atgttgaaac ttttataaaa ctaaaaaata
     5281 tttttttatt tctataaaat atcttactct ggtgtaaaat acttatagct atatattgta
     5341 tgaaagggag acatctcatg aaaaagatta ttactgttct tattatgctt atattttcag
     5401 ctctttccgt gttttcatac ggtctgtcaa tctgcggagg cggagcatat gcttttaagt
     5461 gcataggttt tttgcaggca ctcagcgaag aaggattctg cccagatgaa gtagccagta
     5521 cttcactggg atcaatagcg ggttatttca tggcatgcgg atacgatatg tctcatgtgc
     5581 ataagctttt ttccaagata aactttacag atctttttga tatctctctt ccactgaatg
     5641 gcggtttttt aaactctgca agactaggag actttatcgc attttcgtcc ggttgtgaca
     5701 gctaccttga tatgcagtca gccctaaaaa tggcagcatt aaatatgaac actcttaatg
     5761 tagagtttct agacaaaggc gatcctataa aggctttacg cgcaagtacc gccatgcagg
     5821 gacattttga acctgttgta ataaactctg atttttttgc ggatgcagga gtaaaagagc
     5881 ttagtataat taatcctcag gagatttttt gctcagacga aaactttctc atacgactta
     5941 ctccttcata tcgtgatact tcggatattg attttagaaa tattataaac gttatctttg
     6001 cagcaataga agccggaggc ataaaccggg atctttacgc ggcagataag ctttcataca
     6061 atgaaatctt tgatattgaa agttctattc ccatagatgt aaaatttttc agtaagggcc
     6121 ttgtttttta taatgatgga tatcttgccg ggaaaaagtt tttgaatgaa aatccggatt
     6181 tttttgaaag aaataatata ccccaaaaaa acattcaaaa aaagacactt gatattgaaa
     6241 gtatctgcac gtcgttaaga acagagtcat acttacggcc acgtgaattt tactggacag
     6301 taaaagccga tattcatcaa gaaaattctg acaagtttgt acgctcatat atatactcag
     6361 agtttcttaa ttccagaatc cgtgcgggaa tagcttataa atttaatgat acgctctacc
     6421 catacataag ccttcagaca tactattttc ctttgcgatg cttgcgcttt accgcagagt
     6481 atgttcataa tatgagatac ggtataactc tacggacata ccttatggaa aagtataaaa
     6541 gtattgttta cgccgacctt tcatataccc atggcataaa ggatgacaac ctttttactt
     6601 tcggactgac atttgatgga atgtacagct ataactcggc taatcttggt cctgaactgt
     6661 cgctgaagat actttctgat ctaaacatta taaatggtat gctttctatc aagaatatct
     6721 atggtgatga ttatatgaat ctgtcagtaa gctcttccta taattataaa aataatttta
     6781 accagatacc agatatctta tgcaagtatc cctttattca taagatatcc caacagtatg
     6841 ctttaaaaat accgttgata aaaaatctcg atatcgacat gtttcagact ttcctgctcc
     6901 aggatatata cggaaaattt tattttaact gggctattga tgattttatc ctaagctctt
     6961 ttcttccttc ctgtggtctg tcgttgttct ttccgttcag attcgcagga gtttccaact
     7021 cttttataga gtgcggtata aattacagag acagcttttc cttctttttt tccctctctg
     7081 actacctttt ttgataagag ttgacagaag aaaaaaataa aggtataatt aagtatacct
     7141 aaaaaggggg agtttatatg gaaaaaatta ttgcctgctg tggacttacc tgcgatacat
     7201 gtgacgcttt taaagcaacc tccatgaatg atgatactct gcgtgaagaa acggccaaga
     7261 aatggggagg catgtacggc gctcaattca aaccaaaaga tattaactgt ctgggctgcc
     7321 attccaatac gcttttcgga tattgcaaca actgcgatat acgtgcatgc tctaaaagca
     7381 aaggctttaa caattgcggt gaatgtactg attacgtttc atgcgaaaaa atcgcgaagt
     7441 tcacggagat ggttccttcg gtaaaaccat tgcttgatca gattcataac tcaacaagat
     7501 aagaccggct aaaagccggt cttatctata tgttaaagca cacttgacat tgctgcacaa
     7561 tgagatgtag tatgatatat tcaagaaaag aacctaaaca aactatctaa ggaggaaagg
     7621 atgatgcaac ttaataatga atatgagtac atttcacaga ccaaagtatt tggaatacct
     7681 cttatacata taaagggtca tggaacagcc aaaggaatag ttgccatagg tcccttggca
     7741 ttaggcataa taagcatagg aatactgccg gcaggtctga tctctttggg agctttaagc
     7801 accggaatac tacttgcaat aggaggacta tcactttctt tatttacagc aattggcgga
     7861 gtagctctga gcttttacct ttcaatagga ggagtcggta tttcgtactt ttactcattg
     7921 ggcggcgcag caatcgcaaa ccactgcgcc ataggcggag cggcaggcgc aaagctggcc
     7981 gtaggagcaa gtgcaaaggg taaccttgcc atcttttcac aaaagggcta cggacagaca
     8041 gtaatagatt atacaaaggt atcagcagag tatgtccttc aaagcataaa ggagttgtat
     8101 ccatatatat caggatggtt tattgaactt gtaaaattga ctatacgctg aaaacagcct
     8161 tttactcaag gctatttaaa aattttttta tttgaactgc cgtttgatca tcttctggta
     8221 caagagacaa aaatttttta aaatcttttt tagctaaata atattgttcc agatctagat
     8281 acgcatgtcc gcgattcgca taacactctt taatacttgg atccagtttg attgccgtag
     8341 agaaatctgt aatggctgat ttatactgtt tcaaacatag atacgcatat ccgcgattat
     8401 tataaaccaa tgcattgttt ggatccattt tgattgccgt agagtaatct gtaatggctg
     8461 atttgtactc tgataacttt agatacgtat gtccccgaac actatatgcc ggcacatagt
     8521 ttggatctag tgtaattgcc ttagtgtagt caaagattgc aggtatattt tttttcaacg
     8581 ccaaatatgc ggatccacgg ccattataag ctgatgcata gtttgggtct aacgaaattg
     8641 ctttagtgta gtcaaagatg gcaggtatac tttttcccaa agatatataa gctgttccac
     8701 ggccattata agcatgtgta taatttggat ctaatgcaat tgccttagtg taatcagcaa
     8761 tagcaggctc atactgtttc agagccagat aggctgatcc gcgactttca taatattctt
     8821 ttttatttgg atccatt
//
LOCUS       DAOSHR010000079         8749 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_6845_length_8749_cov_5.718772, whole genome shotgun sequence.
ACCESSION   DAOSHR010000079 DAOSHR010000000
VERSION     DAOSHR010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 8749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8749
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6845_length_8749_cov_5.718772"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1145)
                     /locus_tag="PLU28_05675"
     CDS             complement(<1..1145)
                     /locus_tag="PLU28_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOG34590.1"
                     /translation="MKKLLVVLLVLVSAVALIAEPEWLIEDVAGGKKGGVLYLSALSD
                     PKTLNTAWAQETSSTDMITWFLDTLLYSSNTGIADQPGMAKKFWKEETTNGISYFFQL
                     RGGLKWSDGHDLTIDDVIFTFDKIWFNPNMTANGNGAYLDSQERLPKIEKVGTDTVKF
                     TYEEKFRQGYLMFGRAEIYPKHIFEKIVEDPSKFSQTWTVEQVDKVIASGPFMIKEYK
                     PGVRIVFERNPYFYIKSKDGVQLPYLDQIIYLIVPDQNTTRLKFEAGDIDLLAPTATE
                     FPSLKAQATAKKWTTKVGGPALGAQFIAFNFNTEDPIKREVFRDPSFRVALSYAFDKQ
                     SIIDNLYNGLGEALYGPVTRSSGFYTTEVEKLGFRYSLATAKKILKEA"
     gene            complement(1195..2364)
                     /locus_tag="PLU28_05680"
     CDS             complement(1195..2364)
                     /locus_tag="PLU28_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG34591.1"
                     /translation="MLLRVDNLKKYFPLKHGFIVEKTVGYLKAVDGVSFSLDRNQTFG
                     LVGESGCGKTTIGKTILRIYDPSAGRVFLNDEDTSFYFMPKAQAVKFLKNEYVARAKA
                     NLETYKSVENALDNVQDYEKKYFKFFYEKGENAFYDHMLSGIREKRMSFRRAVQIVFQ
                     DPTSSLNPRMTVGQMLSEPLMFHNVVRNRQEASDLVQEILHKVGLKGYHADRYPHQFS
                     GGQRQRVAVARAIILKPQLIILDEPTSALDVSVQAQIIQLLKDLQKEMNAGFLFISHN
                     LGVVRYISDKVGIMYLGRMVEQAASDDIFDRTMHPYSEALLNSAPVPDPKKKRDRKQF
                     IIKGQVPSPINRPQGCFFNPRCKYVMPECKSEYPGFYDIGMQHNVACYKYKEKKV"
     gene            complement(2364..3419)
                     /locus_tag="PLU28_05685"
     CDS             complement(2364..3419)
                     /locus_tag="PLU28_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG34592.1"
                     /translation="MNEPVLKVKDLKTFFKIPEGIVKAVNGVSFDLSTTEALGVVGET
                     GSGKSVAMKSVMGMIRKPGYVVPSSQILFRTNEFSKDGSYEYVNLATLEYETFTRIRG
                     KHIGMVFQDPMASLNPMYTVADQMIETIVFHQGVKIDEARDKAIELLNKVGIANPKER
                     IDDYPFQFSGGQRQRIVIAIGLSCNPEILVADEPTTALDVTIQAQILELMKDLQKEYK
                     SGMIYITHDLSVISEIADKVMVMYGGTQMEIADVNTIFENPMNPYTNALLSCIPRHDI
                     KKEKLEPIPGQPPIMLNPPEICPFLPRCQRATERCRKEWPELTSISENHMVRCFNPQV
                     NKVSISGAKAGGTEGGK"
     gene            3695..3925
                     /locus_tag="PLU28_05690"
     CDS             3695..3925
                     /locus_tag="PLU28_05690"
                     /inference="COORDINATES: protein motif:HMM:NF013633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HOG34593.1"
                     /translation="MRLDKYLADIRIVKRRVVAHDMCESGKVMRNGMALKPGHEIKIG
                     DTLDVQLAKKTLKITVSGEKDFEVIQETIRES"
     gene            complement(3909..5180)
                     /locus_tag="PLU28_05695"
     CDS             complement(3909..5180)
                     /locus_tag="PLU28_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HOG34594.1"
                     /translation="MTGIKPLYEQLRPLKIEDIVGNSQVKKILKNWVNAKRPRSFIIW
                     GPPGSGKSSIISALTKELERDYSVFSFSGALEGTKKIKEILSAGENLFSKQKILFVDE
                     VHRLNRAEQDTLLLGIEKGEIILFGATTENPAVTINPALLSRLLVFKTILLKDEEYED
                     FFLRTENFFNPKIKISQEARKVMIQYSNGDLRRIINIIESLAQNNTDNIDTSDLRSIV
                     DINTSYNEDDKYNFISAYIKSMRASDPDAALLYLSYMLENGEDPMYIARRMVLLSCED
                     IGLADPQAITVAVSAMTATQNIGYPECYLPLAHATLYLSSMPKSNSVNTAYVNAREFM
                     QNNPVHVPPKLINAVNKLMEKQGYGVQYKYPHSYNGFIKESCMPQGLENKIFFSPKEI
                     GFEKKIKERLELLWDGLKKYTDSRKDTNSPL"
     gene            complement(5155..6141)
                     /locus_tag="PLU28_05700"
     CDS             complement(5155..6141)
                     /locus_tag="PLU28_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOG34595.1"
                     /translation="MDLHSELLREVDLMKTQDFKKAKVLVTGGAGFIGSNIVDGLIHM
                     GHSVIAVDSLITGSIENLSPLALFYQQDITDEKVMEKIFNSHKPDFVIHTAAQISVPA
                     SVENPVFDAQQNIMGTLTVLKMCQKYGTKKIIFSSTGGAVYGESVPIPTTEDTCPNPI
                     SPYAIAKLSCEKYIEFYSRQYGLDYTVLRYSNVFGPRQIPKGEAGVVCIFTENMLKNK
                     EINIYGNGEQTRDFIYVGDVVMANLKALMNGSGEKINISTNTPTTINELFKVLSEKIG
                     YSSLPIYKDERAGDIKDSLLSNSKAKKVLSWEPKYDLEKGVGETVDWYKTII"
     gene            6266..8047
                     /locus_tag="PLU28_05705"
     CDS             6266..8047
                     /locus_tag="PLU28_05705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34596.1"
                     /translation="MLKKFFLSALVFFICAVFLVSCAKSSDFKYDILIDNKISPTTPL
                     SITTNFTFSKVNITIDGTKLSLDSSQLPYVFDDCGDGIYDIGMEFYGISNNLLTKYST
                     NIIYDSTSPKIDKIGWSIVSGILHIDMKTTDSDFSHMTVTDLVSGDEFYSDTGKQDIL
                     LRKNTGVIEFLVSVYDAAGNKSTQSIMIDTQIDNKPVLNSKILKMNTFGDIFVDVTDD
                     WDVDPVIFIKKGDNYVYPNQVLCDPSVSSAECIFIDSSNNYITALMQIKKDLTFPSKI
                     ESSPRWISNDLKYLTWRLDDDAAGYVIEGFDPVCGWKLYKETGISYAEVSAQDVMFIR
                     KVSANGTKGLPSPPIYRFSDFILPFSTGVLEDVKQNTMLSQINSPFILSKDIVIEKGK
                     TLFIESGTNIRFFNNSRMIVRGTVFLMHGAARSRLFGDGEIFADGGNIILSNADIENI
                     KITSKSAKFIFLEDLSSDESVEAELTNSLRTCIYNVRADYINFTVKNSSGLFIDNSSL
                     GSVKISNCNEALFSSSSVKNLGFDINSRACFSKMKVSNLSNLNFSYIYMFDSDIGSLT
                     NSSFSMVTSNSSRISQIKENSGVIKSE"
     gene            8040..>8749
                     /locus_tag="PLU28_05710"
     CDS             8040..>8749
                     /locus_tag="PLU28_05710"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOG34597.1"
                     /translation="MSDYFAQEFDTADRVFRSLSISTPCTTHGINHATVFIGAVFLHI
                     YERYGKASKKVAELGSGCGALSLFLARQFPYFKFSCYELQKELHDYAILNSQENNIQN
                     VVFNNVDVSQLYRKDSENVTFNLQKGSFDIVLSNPPHYISSMGVTSEMSDRRIARTFD
                     SPQAMSAFFVSAYTLLRNKGTAIFVLHPNILIDVYEFSRKNGFEPFEQYNAYSSVGKD
                     AQLSVVLFRKNGGKNFIL"
BASE COUNT         2426 a         1659 c         1710 g         2954 t
ORIGIN      
        1 gcctctttaa gtatcttttt ggctgttgcc aaggagtatc tgaatccaag cttttcaact
       61 tctgtggtat agaatcctga agatcgtgta accggtccgt aaagagcctc tcccaatccg
      121 ttgtaaagat tgtcgattat ggactgtttg tcaaaagcat aagaaagagc tactctgaaa
      181 ctcggatctc tgaatacttc tctctttata ggatcttcgg tattaaagtt gaaggctata
      241 aactgtgctc ccagagccgg tccgccgact ttggtggtcc atttctttgc tgttgcctga
      301 gctttcagag acggaaactc agttgctgta ggagccaaaa ggtctatatc tcctgcttca
      361 aacttaagtc ttgtggtgtt ctgatcaggg acgataaggt atattatctg atcaagatac
      421 ggaagctgta caccgtcttt tgactttata tagaagtacg ggtttctttc aaatactatt
      481 cttacaccag gtttatactc ttttatcata aaaggaccgg atgcaataac tttgtctact
      541 tgctcaactg tccatgtctg actgaacttt gatggatctt caacaatctt ttcaaagata
      601 tgcttgggat atatctcggc tcttccgaac ataagatagc cctgtctgaa cttttcttcg
      661 taggtgaact ttacagtgtc tgttcctacc ttttcaatct taggaagcct ttcctggctg
      721 tcaaggtaag ctccgtttcc gttagcggtc atattcggat tgaaccatat cttatcaaaa
      781 gtaaatatga catcatcaat cgtaagatca tgaccatcac tccacttaag tcctcctcta
      841 agctggaaga aataagatat tccgtttgtg gtctcctctt tccagaactt cttggccata
      901 cccggctggt cagcaatacc tgtgttgctt gaataaagca gagtgtcaag gaaccatgtg
      961 atcatatctg ttgacgaagt ttcctgtgcc catgcggtgt taagtgtctt tggatcagac
     1021 agtgctgaaa ggtaaagcac tccacctttc ttacctccgg caacatcttc aatcagccac
     1081 tctggttcag ctatcaatgc tactgcactt actaatacca ataaaactac taacagcttc
     1141 ttcatactat gccctcccct ctgagttata gttaattatc cgctgtttat ctatttaaac
     1201 ttttttttct ttgtacttgt agcaggctac gttatgctgc attccaatgt cgtaaaaacc
     1261 aggatactca cttttgcatt ccggcataac gtacttacat cttggattga agaaacatcc
     1321 ctgcggtctg ttgatgggac taggaacctg tccttttatt ataaactgct ttctgtccct
     1381 ctttttctta ggatcaggaa ctggagcaga gttcaaaaga gcttctgaat aagggtgcat
     1441 ggttctgtcg aatatatcgt ctgaggctgc ctgttcaacc attcgtccta agtacattat
     1501 cccaacttta tcagagatat accgtacaac acccaggtta tgagatataa aaaggaaacc
     1561 ggcattcatt tctttctgca gatctttcag gagctgtatt atctgtgcct gaaccgaaac
     1621 atcaagagcc gaggtcggtt cgtcaagtat aatcagctga ggttttagaa ttattgctct
     1681 tgctactgct actctctgtc tctgtccgcc agagaactgg tgcggatatc tgtcggcatg
     1741 atagccttta agacctacct tgtgcagaat ttcctggaca agatcgctgg cctcctgtct
     1801 gtttcttact acattatgga acatgagagg ttcactcagc atctgtccaa cggtcattct
     1861 tggattaagc gatgaagtcg gatcttgaaa aactatctgg actgcacgtc tgaagctcat
     1921 tcttttttct cttattcctg aaagcatatg atcgtaaaaa gcattttcac ctttttcata
     1981 aaaaaactta aagtacttct tttcatagtc ctgcacgttg tctaaagcat tttcaacact
     2041 tttataggtt tcaaggttgg ctttggctct tgctacatac tcattcttca ggaactttac
     2101 tgcctgtgcc ttaggcatga agtagaagga tgtatcttcg tcattcaaaa agactcttcc
     2161 ggcagatgga tcgtatattc tgagtatggt tttgcctatg gtggtttttc cgcaacctga
     2221 ttcaccaaca agtccgaatg tctggtttct gtcaagagaa aatgacacac catctacagc
     2281 cttaaggtaa cctacagtct tttcaactat gaatccgtgt tttaatggaa agtacttctt
     2341 aagattatca actcttaaaa gcattattta cccccctccg ttccgcctgc tttggcaccg
     2401 cttatgctta ctttgtttac ctgcgggtta aagcatctca ccatatgatt ttcagatatg
     2461 ctggtaagtt ccggccactc ttttctgcat ctctccgttg ccctttgaca tcttggcagg
     2521 aaaggacata tttccggagg attaagcatt atcgggggtt gtcccggtat tggttccaat
     2581 ttttctttct tgatgtcatg acgtggaata catgacaaca gagcgttggt gtaagggttc
     2641 atcggatttt caaaaatggt attaacatcg gcgatttcca tctgggtacc gccgtacatg
     2701 accattacct tatctgcaat ttctgatatt acagaaagat cgtgggttat gtaaatcatg
     2761 ccgcttttat actctttttg cagatccttc atgagttcga gaatctgagc ctgtatggta
     2821 acatcaagag cagtggtagg ttcatcggca acaagaatct caggattgca tgaaagacct
     2881 attgctataa caattctttg cctctgtcct cccgaaaact ggaaaggata atcatcaatc
     2941 ctctcttttg ggttagctat accaacttta tttaaaagtt cgattgcctt atctctggcc
     3001 tcatctattt tgactccctg atggaacaca attgtttcta tcatctggtc ggcaactgtg
     3061 tacataggat taagcgaagc cattggatct tggaaaacca tacctatatg ttttcctctg
     3121 attctggtga aggtttcata ttccagagta gcaaggttaa catactcgta tgaaccatct
     3181 ttgctaaact catttgttct gaaaaggatc tggctggacg gaacaacgta tccgggtttt
     3241 cggatcatgc ccattaccga tttcattgct acgcttttac ctgaccctgt ttctccaaca
     3301 acccctaaag cctcagtagt gcttaagtca aaagacacac cgttaacagc tttgacgata
     3361 ccttcgggta ttttgaagaa tgtttttagg tctttaacct tcaatactgg ttcattcaaa
     3421 tttctcaccc ctgcggtttt atttatcctt tttatattat aatagatatg aactttaaat
     3481 gtcaagtgtt acgaagatat tatgtaaagg ataatttata atacgttaac ctaacgaatt
     3541 tacttcgtat ctttcataga ttataataga tatgaacaat aaatgtcaag tcttgtgaac
     3601 ggaaaaatta aggtgtaaat tcaaaaaaac tttacataat catattaaat ctttattatg
     3661 ctcaaagcac tcatatatta ttcaggaggt ttttatgaga ctggacaagt atcttgctga
     3721 tataagaata gtaaaaagaa gggtagtagc tcatgatatg tgtgaatccg gaaaggtaat
     3781 gcgaaatgga atggctctta agccgggaca tgaaataaaa atcggcgata cgctggatgt
     3841 acagctggcc aagaagactc ttaagataac agtctcaggt gaaaaagact ttgaagttat
     3901 tcaggaaact ataagggaga gttagtatcc tttctcgagt cagtgtactt ttttagtcca
     3961 tcccaaagta attcaagcct ttcttttatt tttttctcaa aaccaatttc ttttggggag
     4021 aaaaaaatct tgttttccag accctggggc atgcagcttt cttttataaa gccgttgtag
     4081 ctgtggggat atttgtactg gacaccgtaa ccttgttttt ccatgagctt gttgacggca
     4141 ttgataagct ttgggggaac atgaaccggg ttattctgca taaactctct tgcatttaca
     4201 tatgctgtgt ttacggagtt ggactttggc atggatgaaa ggtacaaagt ggcatgtgca
     4261 agagggagat agcattcggg gtatccgata ttctgagttg ctgtcatggc agaaacagcc
     4321 accgttatgg cctgaggatc agcaaggcct atatcttcac atgacagcag taccatgcgg
     4381 cgtgctatgt acatgggatc ctctccgttt tcaagcatgt aggaaagata cagcagtgct
     4441 gcatcgggat cacttgcacg catgcttttt atgtaggcgg atatgaagtt atatttgtcg
     4501 tcctcattat agcttgtgtt gatatcgact atactccgca ggtcactggt gtcgatatta
     4561 tcagtgttgt tttgggctaa agattctata atatttatta ttctgcgcag gtcgccgttg
     4621 gagtattgta tcattacctt tctggcttcc tgactgattt tgatctttgg attaaagaag
     4681 ttttctgtcc tcaaaaaaaa gtcttcatac tcttcgtctt taagtaaaat ggttttaaat
     4741 acaagcaggc gtgaaagaag agcgggattt atagtaacgg cagggttttc cgttgtcgct
     4801 ccgaagagaa ttatttctcc cttttctata cctaaaagaa gggtatcctg ttctgccctg
     4861 ttgagtctgt gtacttcatc aacaaataaa attttttgtt ttgaaaaaag gttttctcct
     4921 gctgaaagta tttctttaat ctttttggtg ccttcaagtg cacctgaaaa agaaaaaaca
     4981 gaatagtctc tttcgagttc tttggtaaga gcagaaataa ttgaggactt cccgcttccg
     5041 ggaggccccc atattataaa agaacggggt ctttttgcat tgacccaatt ttttaatatt
     5101 ttttttactt gggagttgcc tacaatatct tctattttta aggggcgcaa ctgttcatat
     5161 aatggtttta taccagtcaa ctgtttctcc aactcccttt tcaagatcgt attttggttc
     5221 ccagctaaga acctttttgg ctttactatt tgatagaagg ctgtctttaa tgtctcccgc
     5281 cctttcatct ttatatattg gaagggaact gtaccctatt ttttctgata gaactttgaa
     5341 aagttcgttt atagttgtag gggtattagt ggatatgttt attttttcgc ctgagccatt
     5401 cataagagct ttaaggtttg ccataacgac atcgcctaca tagatgaagt ctcttgtctg
     5461 ttctccgtta ccgtatatgt ttatttcttt gttcttcagc atattttctg tgaagatgca
     5521 gactactcct gcttctcctt ttggaatctg ccgtggtccg aaaacattgg aataacgcag
     5581 aacggtatag tcaaggccgt actggcgcga atagaactct atatactttt cacatgaaag
     5641 tttggcgatt gcataaggac ttatcgggtt ggggcaggta tcttctgtgg tgggtatagg
     5701 tacggattca ccgtaaactg ctccgcctgt tgaggaaaaa attatttttt tggttccgta
     5761 cttttggcac atcttcagaa ctgtcagtgt tcccataata ttctgctgag catcgaaaac
     5821 aggattttca acagaagctg gaacagatat ctgtgcagca gtatggatta caaaatcagg
     5881 tttatgtgag ttgaaaattt tttccatgac tttttcatct gttatatcct gctggtaaaa
     5941 aagagcaaga ggagaaaggt tttcgatact gccagtaata agactgtcta cggcaatgac
     6001 gctgtgtccc atatgtataa gaccatcaac aatattagaa cctatgaaac cagctccgcc
     6061 ggtaacaagg actttggctt ttttaaaatc ttgtgttttc atgaggtcaa cttctctaag
     6121 aagttctgag tgtaagtcca tttttccctc ctattaattt tatgtcaact atattttacc
     6181 acattttaga tttgccgcag aatcatacgt gtaatactgt gatataattt ctcatatact
     6241 gcagattttt gagaggtgct agtgtgtgtt aaaaaagttc tttttatcgg cactggtttt
     6301 ttttatttgt gccgtttttc ttgtgtcatg tgcaaaatca agtgatttta aatatgatat
     6361 ccttatagat aataaaattt ctccgacaac cccattgtcc ataacaacta attttacctt
     6421 ttcaaaagtc aatattacta tcgacggtac caaattaagt cttgattctt cgcagctgcc
     6481 atatgttttt gatgactgcg gtgatggaat ttatgatatt ggtatggaat tttatggaat
     6541 ttctaataat ctgctcacca agtattcaac aaatataatt tatgacagta cttccccgaa
     6601 aatagataaa attggttgga gtattgtttc agggatactt catattgata tgaagaccac
     6661 agactctgat ttttctcaca tgactgtgac agatcttgta tccggtgatg agttctattc
     6721 tgatacggga aagcaggata ttttgttgcg taaaaatacc ggagtgatag agtttttggt
     6781 ttctgtttat gatgctgcag gcaataaaag tacccaaagt attatgatag acactcagat
     6841 tgataataag ccagttttga attcaaaaat tcttaagatg aatacttttg gagatatttt
     6901 tgtggatgtt acggacgatt gggatgtaga tccggtgata ttcataaaaa aaggcgataa
     6961 ctacgtttat cctaaccaag tactgtgtga tccttcggta tcaagtgctg agtgcatttt
     7021 catagattct tcaaataatt atataactgc acttatgcag ataaaaaaag atcttacttt
     7081 tccttcaaaa atagagtcgt cgccgcgctg gatatctaat gatcttaagt atcttacatg
     7141 gcgtttggat gatgatgcgg ctggatatgt gattgaaggc tttgatccgg tttgcggatg
     7201 gaagctttat aaggaaaccg gtatatctta tgcagaggtc tccgcacagg atgttatgtt
     7261 tatacgtaaa gtatcggcaa acggtactaa aggtcttcct tctccgccta tctacagatt
     7321 ttctgatttt atacttcctt tttccaccgg agttcttgaa gatgtaaagc aaaatactat
     7381 gcttagtcag atcaactctc ctttcatact ttcaaaagat attgttatag aaaagggtaa
     7441 aacactcttc atagaatcag gtactaatat caggtttttc aataattcaa gaatgattgt
     7501 aaggggaacc gtttttttga tgcatggtgc agcacggtca agactttttg gagacggtga
     7561 aatatttgct gacggcggta atattattct ttccaatgca gatattgaaa acataaaaat
     7621 tacttcaaag tcagcaaagt ttatttttct ggaggacctt tcgtctgatg aatctgtgga
     7681 agccgaactg acaaattcat tgcgtacatg tatatacaat gtcagagccg attatataaa
     7741 cttcacagta aaaaattctt caggcctttt tattgataac tcatcactgg gttctgtaaa
     7801 aatatcaaac tgtaatgaag ctcttttctc atcatcatca gttaaaaatc tcggttttga
     7861 cataaattca agggcatgct ttagtaaaat gaaagttagt aatctttcca atcttaattt
     7921 cagttacata tacatgtttg attcggatat cggtagttta accaacagtt ctttttctat
     7981 ggtaaccagt aactcttcaa gaatatcgca gataaaggaa aattccggag tgataaagag
     8041 tgagtgatta ttttgctcag gagtttgaca cggcggacag agtattcaga tctctatcaa
     8101 taagtactcc ctgtacaact cacgggatta atcatgcgac tgtttttatc ggagcggttt
     8161 ttttacatat atacgaacgt tacggtaaag cttcaaaaaa ggttgcagag ttgggtagcg
     8221 gctgcggtgc gttgagtctt tttttggcga gacaatttcc ttatttcaaa ttttcctgtt
     8281 atgaacttca aaaggagctt catgattatg ccattctaaa ttctcaggaa aataatatac
     8341 agaatgttgt ttttaataac gtagatgttt cgcagctata tcggaaagat tcggaaaatg
     8401 ttacatttaa tttacaaaaa ggtagttttg atattgtgct ttctaatcct ccacactata
     8461 taagcagtat gggagtaaca agtgaaatgt ctgacaggcg tattgccaga acctttgact
     8521 caccccaagc tatgagtgct ttttttgtgt ctgcatatac tcttcttcgt aataaaggaa
     8581 cagctatttt tgttttacat ccaaatattc ttattgatgt ttatgaattt tccagaaaaa
     8641 acggatttga accttttgag cagtataatg cttacagttc agtcggaaaa gatgctcagt
     8701 tatcggttgt gcttttcaga aaaaatggcg ggaaaaactt tatattgag
//
LOCUS       DAOSHR010000080         8703 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_6894_length_8703_cov_6.060939, whole genome shotgun sequence.
ACCESSION   DAOSHR010000080 DAOSHR010000000
VERSION     DAOSHR010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 8703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8703
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6894_length_8703_cov_6.060939"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="PLU28_05715"
     CDS             complement(<1..513)
                     /locus_tag="PLU28_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34598.1"
                     /translation="MSLCTFLSSGINKKLSRIPRSESSFNRFDCTEKSDGRIVVSTVQ
                     RKIRLVSDLSAYMENMEKFILQSKNRNSDLIVFPEYNFFDIFGFIPVMKPLNFLLKFF
                     HSENVQKPDSADQNTLMHDIFFMLAQGIEESMKILYSQLARKYGIFIYSGTFIIKRRN
                     SLYNCGFLFDR"
     gene            complement(510..1340)
                     /locus_tag="PLU28_05720"
     CDS             complement(510..1340)
                     /locus_tag="PLU28_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34599.1"
                     /translation="MKVSSAVFSSSHNRKYCEYNDILCYLDELTDSCCGISSEIVVLG
                     SFFGENMISAAKKKGIIFTYTDYISILCHLSLKKNICICSGGVIVVRKKEKFIESFFI
                     CGGKVVFSQIQLYTSSFERSMGISQGSVLEIFDYNGIGCSIIQSTDTLYPNTFRYAAM
                     QGARLILCPTGFYNSYNKYFQFSGAWNNCQMNNLFCIESGFNGNLYQKSIWGESYIHT
                     PLNMLDFGRISDYDLSQDRLFIYADLNFERLSECSSLFDPLEGLNKNLYEKLYFQGEK
                     "
     gene            1627..5169
                     /gene="nifJ"
                     /locus_tag="PLU28_05725"
     CDS             1627..5169
                     /gene="nifJ"
                     /locus_tag="PLU28_05725"
                     /inference="COORDINATES: protein motif:HMM:TIGR02176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HOG34600.1"
                     /translation="MSRKMKTMDGNTAAAHVAYAFTDVAAIYPITPSSTMAEYVDAWA
                     AEGRKNIFGQTVNVVEMQSEAGAAGAVHGSLAAGALTTTFTASQGLLLMIPNMYKIAG
                     ELLPGVFHVSARAIAAQALSIFGDHQDVMAARQTGFALLASGSVQEVMDIAGIAHLAA
                     IKGKVPFVHFFDGFRTSHEVQKIEVLDYDELSGLIDSAALESFRNSGLNPEHPKVMGT
                     AQNPDIYFQAREASNRFYEAIPDIVAGYMKEITKLTSREYRPFNYYGHPQAEKIIVAM
                     GSVTETIEEVVDYLNSKGEKVGLIKVHLYRPFSAKYFFDVLPQSVKKIAVLDRTKEPG
                     ALGEPLYQDVKALFYNYENRPVIVGGRYGLGSKDTTPNQIKAVFDNLNLYNPKDRFTI
                     GINDDVTFTSLELKEKINVASDGTIRCKFWGFGSDGTVGANKDAIKIIGDETDMYAQG
                     YFAYDSKKSGGVTISHLRFGKKPIRSTYLIDEADFIACHKTSYVHSYDLLDGLKKDGT
                     FLLNTNWTTIEDLDKNLPASMKRYIANNGIHFYVIDATKIGMEIGLGNKINMIMQSAF
                     FKLAKVIPMEDAAKYLKAAIEHTYGKKGEKVLEMNYKAVDMGASALIKIDVPSSWKNA
                     KDVSIEEIVKNKTTFVKEIADVMNRMEGDKLPVSAFTGRENGQFPVGTAAYEKRGIAV
                     LVPQWQPQNCIQCNQCSFVCPHAAIRPFILNEEEAAKAPASFETKVPTNPKLKDFKYR
                     IQVSVMDCTGCGNCADICPAKEKALIMKPLETQLDPQIGNWEFATSVSEKKDVLPVDN
                     VIGSQFSKPLLEFSGACAGCGETPYAKLITQLFGDRMVISNATGCSSIWGASAPSMPY
                     CKNSEGKGPAWANSLFEDNAEHGFGIHVAMKQMRNRIADLAQELIGLDICDCIKAPFK
                     AWLDGKEDAKASKAATLEILKVLDKDTSNARANEILKEISSKKDHLIKKSVWIFGGDG
                     WAYDIGYGGLDHVLASGEDVKVLVFDTEVYSNTGGQSSKSTPVGAIAKFAASGKKTKK
                     KDLGRMAMTYGYVYVAQVAMGADKNQLMKAVTEAEKYNGPALIIAYAPCINHGLKAGM
                     GKTQEQEKKAVESGYWHLYRYNPVLKNDGKNPFTLDSKEPKSPFRDFLMSEVRYTSLI
                     LQSKEEAERLFAKAEIDAKERYENYKKMNDGTSV"
     gene            complement(5250..7298)
                     /locus_tag="PLU28_05730"
     CDS             complement(5250..7298)
                     /locus_tag="PLU28_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOG34601.1"
                     /translation="MGKLLEIFNYLDYRKNSGKTDYAVPKRWMPENYIGDVKLKGRKF
                     FVNPYEYYTKIIRSMASEENIKNCLSCSLSSAKDEKDPSWLKKSVIYSAHVRMTGAYI
                     HSEKELFKPIDELGYRESGTFLKMIAMLPYLKRYNVNCVYLLPVTQSSNRFKKGEVGS
                     PYAVKDFFKVDRDYHDPLLGENFSADDEFAAFVQACHLMGIRVMLDFVPRTAARDNKL
                     ILEHPEWFYWIDTKELSSFKPPWIDTLKFELPTMENIESIYSNASVKNHLKKFRMDPK
                     TQSPEKWGNFIKANIDNDNFLDEIMKEFKVITVPGFSDWINDPQPTWDDVTFSRLYLS
                     HPSAAKKFLDPNQPPYVLFDVVKASNFPGEIKNEELWEKLSDIMPFYQTKYGIDGARL
                     DMGHALPKELEHRIIQKAKDTDPSFVVIAEELNMDNHKKAKESGYDAILGNSWWTMPR
                     YMQRTNDAPKGWMKKFISEMMPNLELPAFATSETPDSPRAVTREGGENFSKLSAVLNS
                     FMPNGITVINSGYEIFEAQPMNTGLDFDNPQEQRFQFLPPVDQFYGKLAFFDWYCLHW
                     DTDHHMVKLLQLLGQTKERVKDLIADPKNFRFLEYGEEAFVMFWWEGKTGVVVPVNLN
                     PSNPYYFEVDIGYFTWRGYHNIDTALENYRRGESHWEVHDGKLRVTVNPSEARVFIVS
                     "
     gene            complement(7409..8659)
                     /locus_tag="PLU28_05735"
     CDS             complement(7409..8659)
                     /locus_tag="PLU28_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34602.1"
                     /translation="MHKNIIWNAAFSEVDITPDFQTELIGCYRPDSRSQGVLHRLSAQ
                     VLLFENNNDFYCLTAIDSLGLTVSLSNKLRSAIAEKLNTQLSHIMLNFSHTHSAPAPL
                     SPLNGERYFCFLTEQIMKCVETAKQNLCPFKLSWSVTDTQIGENRRDGCSVVDRRLGA
                     LKLEKLNGIPIAVVLRITAHANILMSCNNQISGDYFVPAREKLGRYFKCPVMLIQGAA
                     GNIKPAGTDKILGGSVDDLEKITDILLDSAKKLKFDPDEVRKIFMYSSEFDYYSDIPC
                     ETEAESIAAKAKQLFDIDGTQWLNECRSLREREVSQQHQKGEVQFLYINEGCICGVAD
                     ELFCEISIDVMEKTESPFMFFNGYTNGCTGYLPHAEEWEKGGYETLYSYLQYYMFHGH
                     VMPFRKDTAKRLITLVSDEWKKNF"
BASE COUNT         2726 a         1749 c         1720 g         2508 t
ORIGIN      
        1 tctgtcaaat aaaaatccgc agttatacag actgttcctg cgttttatta tgaaggtacc
       61 gctgtaaata aagattccat acttccgtgc aagctgtgaa tacagtattt tcatcgattc
      121 ttcaattcct tgtgcaagca taaaaaaaat atcgtgcata agtgtattct gatcagcaga
      181 atcaggtttc tggacatttt cagaatggaa aaatttcaat aagaaattca atggtttcat
      241 aacaggtata aacccaaata tatcaaaaaa attgtattcc ggaaaaacaa taagatctga
      301 attcctgttc ttggactgca ggataaactt ttccatattt tccatataag cagaaagatc
      361 agaaaccaga cgtatttttc gctgtacggt gcttacaacg attctcccgt ctgatttttc
      421 agtacagtca aagcggttaa atgatgactc agaacgtggt atgcggctaa gttttttatt
      481 tatccccgat gaaaggaaag tacataaact catttttccc cctgaaaata taatttttcg
      541 taaaggtttt tatttagtcc ctcgagaggg tcaaacaaag aagagcattc agaaagtctt
      601 tcaaaattca gatcagcata tatgaaaagt ctgtcctgag aaagatcgta atcagaaatt
      661 cttccaaaat caagcatatt caaaggcgta tgaatgtatg actctcccca gatgcttttc
      721 tgatagagat taccgttaaa accactttca atacagaata aattgttcat ctgacagttg
      781 ttccatgcac cagaaaactg aaaatacttg ttgtagctat tataaaatcc tgttggacag
      841 agtataagcc tagctccctg catggctgca tatctgaagg tattaggata taaagtgtct
      901 gtagactgta ttatagaaca tccgataccg ttgtaatcaa aaatttccag cacacttccc
      961 tgggaaattc ccatagatct ttcaaaagaa gaagtataaa gctgtatttg tgaaaaaaca
     1021 accttcccgc cacaaatgaa aaaagattct ataaactttt cttttttacg tacaacaata
     1081 actccgccgg aacagatgca gatgtttttt ttcagggaca gatggcatag tatacttata
     1141 taatcagtat aggtgaatat tattcctttt ttcttagcgg cagatatcat attttcgccg
     1201 aagaaactgc ccagcacaac aatttccgaa gaaataccgc agcaggaatc ggtcagctcg
     1261 tcaaggtaac acaaaatatc gttgtactcg cagtacttcc ggttgtggct gctgctgaaa
     1321 accgcacttg atactttcat aataatcacc cgcaccagtc gtagataatt aaattatatc
     1381 agaaataaat ttaaagcata aatataaact ttatcgtaac tgttgaaact gtaaatctat
     1441 atgtcatatt ctatttacat cttttaagtg aataatttaa gtctatgggc taaaataaaa
     1501 ataagtaaat ttttattttt cttgatgtta aaacatgtga aaacagtggc aaagtacgtc
     1561 cgtcaaaatg cataaaatag gaaggtaata aataaatctc actataacat ataaggaggt
     1621 aaaaagatgt cgcgtaaaat gaagactatg gacggtaaca cagcagctgc acatgttgca
     1681 tatgctttta cagatgttgc tgccatttat ccgataaccc cttcatccac aatggctgag
     1741 tatgttgatg cttgggctgc ggaaggaaga aaaaatattt tcggacagac agtaaatgtt
     1801 gttgaaatgc agtcagaagc aggtgctgct ggagcagttc acggatcgct tgcagcaggt
     1861 gcattaacca caacgtttac agcatctcag ggattactgt taatgatacc aaatatgtat
     1921 aaaatagccg gagaacttct tcccggagtt ttccacgtaa gtgccagggc aatagctgca
     1981 caggctcttt caatatttgg tgaccatcag gatgttatgg cggcaagaca gacagggttt
     2041 gctcttctcg ccagcggcag tgttcaggaa gtcatggata tagccggtat tgcacatctt
     2101 gctgctataa agggaaaagt tcctttcgta catttttttg acggattcag gacatcccac
     2161 gaagtacaga aaatagaagt tcttgattat gatgagcttt caggactcat agactctgca
     2221 gcactggaaa gcttcagaaa tagcggtctt aatcctgagc acccaaaggt tatgggaact
     2281 gctcagaacc ctgatattta ttttcaggca agagaagctt cgaacaggtt ctatgaagct
     2341 atcccggata ttgtagccgg atatatgaag gaaataacca agcttacctc aagagagtac
     2401 agacctttca actactacgg acatcctcag gctgaaaaaa ttattgttgc aatgggttct
     2461 gtaacagaaa ccattgaaga ggttgttgac tatctcaatt caaagggcga aaaggtcggt
     2521 ttaattaaag ttcatctcta caggccgttc tctgccaagt acttctttga tgtccttccg
     2581 cagtctgtaa aaaagattgc tgttcttgac aggacaaagg aaccaggcgc actcggagaa
     2641 ccgctttacc aggatgtaaa ggcacttttc tataattatg aaaacagacc ggttatagta
     2701 ggcggaagat atggactcgg atcaaaggat acaactccta accagataaa agctgttttt
     2761 gacaacctta acctctacaa tcctaaagat agattcacca taggcataaa tgatgatgta
     2821 acctttactt cattggagct gaaagaaaaa ataaacgttg cctctgacgg aaccataaga
     2881 tgtaaattct ggggtttcgg ttcagacgga accgtcggtg ccaacaagga tgccataaag
     2941 ataatcggag atgaaaccga tatgtatgcc cagggatact ttgcatatga ttcgaagaag
     3001 tccggcggcg ttaccatatc gcatctaaga ttcggtaaaa aaccaatacg ttcaacatat
     3061 ctcatagacg aggctgattt catagcctgc cacaaaacat catatgtcca tagttacgat
     3121 cttcttgacg gtctaaagaa ggacggcact ttccttctta atacaaactg gacgacaata
     3181 gaagatctgg acaaaaacct tcctgcatca atgaaaaggt atatcgccaa caacggtatt
     3241 catttttatg tgatcgatgc tacaaaaatc ggaatggaaa taggtcttgg aaacaagata
     3301 aatatgataa tgcagtcagc cttctttaag cttgcaaaag taattccgat ggaagatgcc
     3361 gcaaagtatc ttaaggcagc catcgaacat acttacggca aaaaaggcga aaaagtgctt
     3421 gagatgaact ataaagcagt agatatggga gcatcggcac ttataaaaat agatgttcca
     3481 agcagctgga agaatgcaaa agacgttagc atagaagaaa tagtcaagaa taagacaaca
     3541 ttcgtaaaag agattgccga cgttatgaac agaatggaag gagacaaact ccccgtttct
     3601 gcttttacag gcagagaaaa cggacagttc cctgtcggaa cggcagcata tgaaaagaga
     3661 ggaattgccg ttcttgttcc gcagtggcag ccacagaact gtatacagtg taatcagtgt
     3721 tcatttgtat gtccgcacgc agctataaga ccgtttattc tcaatgaaga agaagctgca
     3781 aaagctcctg cttcatttga aaccaaagtt cctaccaatc cgaagttaaa agatttcaaa
     3841 tacaggattc aggtaagcgt tatggactgt acaggttgcg gtaactgtgc agatatatgt
     3901 ccggcaaaag aaaaagcact tattatgaag cctttggaaa ctcagcttga tccacagata
     3961 ggcaactggg agtttgctac tagtgtatcg gaaaagaaag atgttcttcc tgttgacaat
     4021 gtaataggaa gccagttctc taagccttta ctcgaattct cgggagcatg tgcgggctgt
     4081 ggtgaaacac cttatgcaaa acttattacc cagttatttg gcgacagaat ggttatctcc
     4141 aatgcaacag gctgttcttc gatctgggga gcatcagcac catccatgcc atactgcaag
     4201 aactccgaag gaaagggacc ggcatgggct aattcactgt ttgaagataa tgccgaacac
     4261 ggattcggaa tacatgttgc catgaaacag atgagaaaca ggatcgcaga ccttgcccag
     4321 gaacttatcg gacttgatat atgcgattgc ataaaggcac cgttcaaagc atggcttgat
     4381 ggcaaggaag atgctaaggc atcaaaggcc gctactcttg aaattcttaa ggttcttgat
     4441 aaagatactt caaatgccag ggcaaacgaa atactcaaag aaataagcag caagaaagat
     4501 catctcataa agaagtccgt atggatattc ggaggagacg gttgggctta cgatatagga
     4561 tacggaggac ttgaccatgt tcttgccagc ggtgaagatg taaaggttct tgtatttgat
     4621 accgaagttt attcaaatac cggtggacag tcatcaaaat ccactcctgt gggggctata
     4681 gctaagtttg ccgcttcagg aaagaaaacc aagaaaaaag atctcggaag aatggcaatg
     4741 acatatggat atgtctatgt tgctcaggta gccatgggcg cagacaagaa tcagcttatg
     4801 aaagcagtga cagaagccga gaagtataac ggaccggcac ttataatagc atatgcacca
     4861 tgtataaacc atggacttaa agcaggtatg ggcaagacac aggaacagga aaagaaagcc
     4921 gttgaatcag gatactggca tctttacaga tacaaccctg ttcttaagaa cgatgggaag
     4981 aatccgttca ctcttgattc caaagaaccc aaatcacctt tcagagactt tttaatgagc
     5041 gaggtaagat acacatctct tatcctccag tcaaaggaag aagcagaaag actctttgcc
     5101 aaagccgaga tagatgccaa agagagatat gaaaattata aaaaaatgaa cgacggtaca
     5161 tctgtatagt aataaataag aacagccgca catttaaatg tgcggctgtt ttctttgtat
     5221 tttcattggc gataataatt ctatactgat taagatacga taaaaaccct tgcttcagac
     5281 ggatttacag ttactctgag ttttccgtca tgtacttccc aatggctttc tccacgtcgg
     5341 tagttttcaa gtgccgtatc gatgttatga tatcctctcc atgtgaagta ccctatatcc
     5401 acttcaaagt aataaggatt gctcggatta aggtttaccg gtactaccac tcctgttttc
     5461 ccttcccacc agaacatcac aaaggcttcc tcgccatatt caagaaatct gaagttcttc
     5521 ggatcggcaa taagatcctt gactctttct ttagtttgtc ctaaaagctg gagcagtttt
     5581 accatgtgat gatctgtatc ccagtgtaga cagtaccagt cgaaaaaagc caactttccg
     5641 taaaactggt caacaggtgg cagaaattga aaacgttgtt cttgtggatt atcaaaatca
     5701 agaccggtgt tcataggctg agcctcaaat atttcgtaac cactgtttat tacagtaata
     5761 ccgttaggca taaaggaatt tagtactgct gatagcttcg aaaaattttc tccgccttct
     5821 ctggttacag ctcttggaga atcgggagtt tcagatgttg caaatgctgg aagttcaagg
     5881 ttaggcatca tttctgagat gaattttttc atccagccct tcggagcatc attggttctc
     5941 tgcatgtaac gcggcatagt ccaccatgaa tttcccagaa ttgcatcata tccgctttcc
     6001 tttgcctttt tatgattatc catgttcaat tcttcggcga taaccacgaa cgaaggatca
     6061 gtatcttttg ctttttgtat tatcctgtgt tcaagctctt ttggaagagc atgtcccata
     6121 tcgagccttg caccgtctat tccgtacttt gtctggtaaa acggcattat atcagaaagt
     6181 ttttcccaaa gctcttcgtt tttgatttct ccagggaagt tgcttgcctt tacaacatca
     6241 aagagcacat aaggcggctg atttggatca aggaactttt tggcggccga aggatggctt
     6301 agataaagcc tagaaaacgt tacatcatcc catgtcggct gagggtcgtt gatccagtct
     6361 gaaaatcccg gaaccgtaat cactttaaac tctttcataa tctcatcaag aaagttatcg
     6421 ttgtcaatat ttgcttttat gaagtttccc catttttcgg gactttgagt ctttggatcc
     6481 attctgaatt ttttcaggtg atttttgacc gatgcattac tgtatatact ttctatattt
     6541 tccatggtag gcagttcaaa cttaagagta tctatccatg gtggtttgaa agaagaaagc
     6601 tctttggtgt ctatccagta gaaccattcg ggatgctcaa gaatcagctt attatctcgc
     6661 gctgctgtcc ttggtacaaa atcaagcatt acccttatac ccataagatg acacgcctgc
     6721 acaaaggcag cgaactcatc gtctgcggaa aaattttcac ccagaagcgg atcatgataa
     6781 tccctgtcaa ccttgaaaaa atcttttaca gcatagggag aaccaacttc acctttttta
     6841 aacctgttgc tggactgtgt aaccggaaga agatatacac agtttacatt gtatctttta
     6901 agatagggaa gcattgctat catcttcaga aaagttcccg attctctgta tccaagctca
     6961 tcaatgggtt taaagagttc tttttcgctg tggatatatg ctcccgtcat tcttacatgt
     7021 gcgctgtaaa ttacactttt tttcagccaa gacggatctt tttcatcttt tgcggaggaa
     7081 agagagcagg acaaacagtt cttaatattt tcctctgagg ccatgctcct tattatcttg
     7141 gtatagtact cgtagggatt gacaaaaaac tttcttccct taagcttaac atcgcctata
     7201 taattttcag gcatccatct ttttggaacc gcataatcgg tctttcctga atttttcctg
     7261 taatcaagat aattaaaaat ttcaagaagt tttcccattt tccgcctcct tgtctatttt
     7321 atctgaacaa attgtttgac tgccaattat atttattata tcatataaaa agttttaaag
     7381 gcggatgatc aaacataacc cactacgatc aaaaattttt cttccattca tcagacacaa
     7441 gggttataag tctttttgca gtatctttac gaaatggcat gacatgtccg tgaaacatat
     7501 agtactgaag ataactgtat aaggtttcat aaccaccctt ttcccattct tccgcatggg
     7561 gcaggtatcc tgtacatccg tttgtataac cgttaaaaaa cataaaaggc gactcggttt
     7621 tctccatgac atctatggat atctcgcaga aaagctcatc tgcaacacca cagatacagc
     7681 cttcatttat atataaaaac tgtacttcac ccttttgatg ctgctgagag acttcccttt
     7741 cccttaagct tctacattca ttaagccatt gagttccatc aatatcaaaa agttgtttgg
     7801 cttttgcggc aatgctttct gcctctgttt cacatggaat atccgagtaa taatcaaact
     7861 cactactgta cataaaaatc ttcctgacct catccgggtc aaacttaagt ttttttgcag
     7921 aatccagcag aatatcagtt atcttttcca gatcatcgac agatccgccc agtatcttat
     7981 cggtacctgc tggctttata tttcctgctg cgccttgaat aagcattaca ggacatttaa
     8041 agtatcttcc gagcttctca cgggcgggaa caaagtaatc tcctgaaatt tgattgttac
     8101 agctcatgag aatattggcg tgagccgtaa tacgtagaac aacagctatg ggaatgccgt
     8161 tgagcttttc aagttttaaa gctccaagcc tgcggtctac cacactacat ccgtcacgcc
     8221 tattttcacc tatctgcgta tcagttacag accacgaaag tttgaacgga caaagatttt
     8281 gttttgcagt ctcaacacat ttcataatct gttctgtcag gaaacagaag tacctttccc
     8341 cgttaagcgg agaaagaggt gcgggggcag agtgagtatg agaaaaattc agcatgatat
     8401 gtgaaagctg agtgttaagc ttttccgcta tggctgaacg cagcttgtta ctcaaagaaa
     8461 ctgtcagccc aagactgtca attgcagtca ggcaataaaa atcattgttg ttttcaaata
     8521 gaagcacctg tgcgctgagt ctatggagca caccctgtga tcggctgtct ggacggtaac
     8581 atccgataag ttcagtctga aaatccggag taatatctac ttctgaaaaa gctgcgttcc
     8641 aaataatgtt tttatgcata tcaactctcc tgttttatcg tataaaatcg ttacttttga
     8701 aaa
//
LOCUS       DAOSHR010000081         8690 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_6917_length_8690_cov_5.118819, whole genome shotgun sequence.
ACCESSION   DAOSHR010000081 DAOSHR010000000
VERSION     DAOSHR010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 8690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8690
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6917_length_8690_cov_5.118819"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            181..1473
                     /locus_tag="PLU28_05740"
     CDS             181..1473
                     /locus_tag="PLU28_05740"
                     /inference="COORDINATES: protein motif:HMM:NF039831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5693 family protein"
                     /protein_id="HOG34603.1"
                     /translation="MKFEFLAGMRKYLFIALFIFVIAFSVIASIYDVRSMEEYFFFPE
                     EELLEKTVYTGITFYVYNNSVVLKELDEKIMKEASIMQFLSYLNGSYNRIFITEFSDF
                     EKYFTQIKDKIPSSVKNKIFYVHYIKNAEIDKFDDETIVQRFLRAHRERRISYFLMPV
                     NPRTQSIKLAIKNDIGSKENFEIIRYKPYFLITWIGFGFIFSIFMTYNPIIAVVYALT
                     YVFFNSWSFTVAASVFSAVIFFKISSKNVFKIIFFGLLLGTTVYSSAYTYEYIYKLTN
                     VRGVKILLMFLLVLVFIRYFRTISFKQLELKRIIPLFFALIAGVAFYIMRSGNWVFVS
                     NFERKARDILEKILIARPRSKELLSYIFFYTKPFGGMEILWRMMRAVLVISILDTFLH
                     VHSPVYLGVLRTINGFAASLVILVVIELIKKIKTPGIK"
     gene            complement(1517..2713)
                     /locus_tag="PLU28_05745"
     CDS             complement(1517..2713)
                     /locus_tag="PLU28_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOG34604.1"
                     /translation="MNFSKRALQMQASPIRKLIPFSDEAKSKGKKVYHLNIGQPDLPT
                     PQQFYDAVKKFTPKVLAYSHSAGLMELRQAFSQYYKKWNMDFSAEEIIVTNGGSEAIV
                     FALSVVADPGDEILTIEPFYANYRGFCEMLNIKITAARSKAEDGYALPSKHEIESKIT
                     SKTRAILFSNPSNPTGAVYTRHEVQTLVEIAKEHDLYLISDEVYKEFTFDGLKHVSAM
                     EFGEYDRTIIVDSISKRFSACGARIGVLATKNKEVFAQSMKFAQSRLCAPVLEQYGTI
                     GVLNDLGSSYLEEMRQEYQRRRDAVFCELQKIEGAKMKMPKGSFYVSVELPVDDSEKF
                     IKWMLSDFAIENETVMVAPLSGFYTNDEFGKKEIRIAYVLEPDKLKKACYILGEGVKQ
                     YRKTFM"
     gene            3127..3693
                     /locus_tag="PLU28_05750"
     CDS             3127..3693
                     /locus_tag="PLU28_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOG34605.1"
                     /translation="MVDFENTVYVAIDFETTGLRPDLGDRIIEIAAFPIYKGQIRTDY
                     SFYSLVNPEVTIPKEVSHYHKLNNIHIADAPLLSEVIKDFKNYLSDSIIISHNAAMDL
                     RFMDVATKEAGLLPISNYYADTLKMSMYFFDEGPYNLGYLSKKLNLGINSFHRACDDA
                     MATARLFLKLVKKYSVRNLGDFLKRWEG"
     gene            3695..4981
                     /locus_tag="PLU28_05755"
     CDS             3695..4981
                     /locus_tag="PLU28_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOG34606.1"
                     /translation="MIKNYVIDTNVLIHDPRAIFKFQDNNIFIPFPVIEEIDKLKTRR
                     DKVSESARSINRILDGLRGEGNLKDGIKLENGGFLRVMAVEPQKSIDIPRYLGTSPDD
                     SILYYAMYIKKMDTNISTILVTKDLNLRIKADSIGIVAQDYLSDKVEIDKLYRGYAFH
                     TGKPFKTDEYEDIPPDLLELDESVFPNLYLWLNERPYRYNGHKNVFSPVNTSYISEVF
                     GIRPKNYEQIFAFDALLDPSIPLVSLVGIAGTGKTLLSVAAGLKMILESKFYKKMLVS
                     KPVIPMGKDIGYLPGSIEDKMKPWLQPVYDNLEFLCSLKGKKVDEYLSKKDIIEVEVL
                     SYIRGRSIPNQYIIIDEAQNLTPHEIKTIITRAGEGTKIVLTGDPFQIDNPYLDNSSN
                     GLVYVASKFKGSPLAAHVSMFKGERSELASKAAEIL"
     gene            4995..5237
                     /locus_tag="PLU28_05760"
     CDS             4995..5237
                     /locus_tag="PLU28_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4911 domain-containing protein"
                     /protein_id="HOG34607.1"
                     /translation="MTDFREYDIYVKIDQEKIHIVTYLLESQAHLMSVRNRQENGLLK
                     IIVPGPLKDEAIKLINSMTEQVELEVVKVEPHNGVV"
     gene            5212..6282
                     /locus_tag="PLU28_05765"
     CDS             5212..6282
                     /locus_tag="PLU28_05765"
                     /inference="COORDINATES: protein motif:HMM:TIGR01668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HOG34608.1"
                     /translation="MSHTTGLFKRIPVPNEKCRDIYCIDYKTLRILGFKTVLFDYDFT
                     ITGWNQKKITEKTLDLFERLLDMGYQVAIVTNAPYDRVGHVYESTQGRVKVYADMRKP
                     RTAKLSNVLNLMNTTAKESIIVGDLFFTDIMAGNILGLYTVLINPYTYGIDSKVKKMV
                     AFMSRVFYYLFFYTVGWMFRIMDLAVPNEFKKSIRDIDYTMLLEYGYDTVLFDFDNTL
                     MPWHSNEIDAETLQFLRALLNMGFKIMIASNGKDERFSDVRLQLKNMRIEVNGMSMKP
                     LACKLKRKISQAGYSRDKTVLIGDQLFTDVIAGNLCGFYTVKVNPLSSREGFHTKFFR
                     IFEKLVIKSMRRKPNLIRGEQI"
     gene            6279..7388
                     /gene="yqeH"
                     /locus_tag="PLU28_05770"
     CDS             6279..7388
                     /gene="yqeH"
                     /locus_tag="PLU28_05770"
                     /inference="COORDINATES: protein motif:HMM:TIGR03597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HOG34609.1"
                     /translation="MSKCSGCGIEIQNQNQDKAGYIPFSVMTERMAAGDEVLCQRCYR
                     IKHYNKFLPVGMGTDFYQKLEDILQTFNTIVWVIDIIDFEGTFRTDIALKLKNKNLII
                     AVNKADLLPNAVKYEEIKEWVCDRVSQVIRVKRDNIRIISTRSGFGINRLSKMISEIN
                     DTKVAVIGVTNVGKSSVLNEITDAGVTVSSYPGTTLNFIKTRLKDSNIELYDTPGIEP
                     KDRVCDFFDIFTQVKMIPAKEISRKTFRLQAGRVMIVSALCYFKVLPVSESMYDPVFL
                     VYAPQNVSFHETKEERIKDILENRKVIVPPYDSSFSMEGIVFEKHTYEVNEGEDIAIP
                     GLGWISVRRGPLVMELSLPQGVTPVVRSSLIKPHK"
     gene            7410..8000
                     /locus_tag="PLU28_05775"
     CDS             7410..8000
                     /locus_tag="PLU28_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HOG34610.1"
                     /translation="MVLKEPGIIVGVSNRHLHLSQHDLEVLFGAGHTLTPTKPLGQPG
                     QFACEETVSLVGPKGSIEGVRVLGPVRKDTQVEVSKTDSFKLGVNPPVRDSGDVAGSP
                     GLKLVGPVGEVELKQGVIIAKRHIHMFTDDAVHYGVKDKDYVSVMCETHDRKLIFQDV
                     LIRVSEKFALEFHVDIDEANAALLKTGDKIKIIEKF"
     gene            8130..8206
                     /locus_tag="PLU28_05780"
     tRNA            8130..8206
                     /locus_tag="PLU28_05780"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8164..8166,aa:Thr,seq:ggt)
     gene            8213..8287
                     /locus_tag="PLU28_05785"
     tRNA            8213..8287
                     /locus_tag="PLU28_05785"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8245..8247,aa:Gly,seq:gcc)
     gene            8397..>8690
                     /locus_tag="PLU28_05790"
     CDS             8397..>8690
                     /locus_tag="PLU28_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HOG34611.1"
                     /translation="MDREVYRLRRLNLDRLVKLAQVGDNKAMGMVLVKLEPMVKSIIS
                     KYYGTWADFEDFLQVGFVGVMQAVYSYNDSSNTKFTTFAYLNISSEIKSFITYL"
BASE COUNT         2811 a         1381 c         1752 g         2746 t
ORIGIN      
        1 atggagataa agattataac agacaggcaa aaaatctttt tggtttgaaa aggtactttc
       61 tgcattcaac gagactatgt ttcaactaca gaggcaaaag atacgatata agctcacatc
      121 tttcaaagga tcttgaacaa gtattggaaa aaattgaaaa tatggggggt tgagcctgaa
      181 ttgaaatttg aatttttggc aggtatgaga aaatatcttt ttatagctct ttttattttt
      241 gtgatagcat tttcagttat tgcctccata tatgatgtaa gaagtatgga agagtacttt
      301 ttttttccag aagaggagct gctcgaaaaa acagtatata ctggaattac tttttacgta
      361 tataataaca gcgttgttct taaggagctt gatgagaaaa ttatgaaaga ggcttcgata
      421 atgcagtttt tgtcatattt aaatggcagt tataacagga tatttataac cgagttttca
      481 gactttgaaa agtattttac tcaaattaaa gataagattc catcgagtgt aaaaaacaag
      541 attttttatg ttcattatat aaaaaatgca gaaatcgata aatttgacga cgaaactatt
      601 gttcagaggt ttcttagggc gcacagggag agacgcattt cttatttttt aatgcctgtc
      661 aatccaagaa cacaatcaat aaagcttgca ataaaaaatg acataggttc aaaagaaaat
      721 tttgaaataa tcagatataa accgtatttc ttgattacat ggataggatt tggatttatt
      781 ttttcaattt tcatgacata taatccaata attgccgttg tatacgcatt gacgtatgta
      841 ttttttaatt catggtcctt tactgtcgcc gcatcagttt tttcagccgt tatttttttc
      901 aagatctctt ccaagaacgt attcaaaata atattttttg gtcttctttt aggaactaca
      961 gtatattcaa gtgcttatac atatgaatat atatataagt tgaccaatgt ccgcggtgta
     1021 aagatacttc tcatgtttct tttagtgttg gttttcataa ggtatttcag gactatttct
     1081 tttaaacagc ttgaacttaa gagaatcata ccgctctttt ttgctttaat tgccggcgtt
     1141 gccttttata ttatgcgaag cggcaactgg gtctttgtaa gtaattttga aagaaaagca
     1201 agggatatac ttgaaaaaat acttatagca aggccaagga gcaaagaact gttgtcatat
     1261 atattttttt ataccaaacc atttggtggt atggaaatac tctggcgaat gatgcgtgca
     1321 gttcttgtga tttcaatttt agatactttt ctgcatgtgc attctccggt ttatctagga
     1381 gttttaagaa ccattaacgg ttttgctgca tctttagtaa ttttggtagt aattgagctc
     1441 ataaaaaaaa taaaaactcc gggcataaaa tgacatgacc cggagttttt tcttagagga
     1501 tgaaaaatac caagtattac atgaaagttt ttctgtactg ttttacacct tcacctaata
     1561 tataacatgc tttttttaat ttatcaggtt caaggacata ggctattcgt atttcctttt
     1621 ttccaaactc atcattggta taaaaaccac ttagcggagc aaccattact gtttcgtttt
     1681 ctattgcaaa atcggaaagc atccatttta taaatttttc actgtcgtcc accggcagtt
     1741 ctaccgaaac atagaaagag cctttgggca ttttcatttt tgcaccttca atcttttgaa
     1801 gttcacaaaa aacagcgtct ctccttctct ggtactcctg tctcatttct tccaggtatg
     1861 aactcccaag atcattcagt actcctatcg ttccgtactg ttcaagtaca ggagcgcaga
     1921 gtcttgattg tgcaaacttc atggattgcg caaacacttc tttgttcttt gttgccaaga
     1981 cacctattcg tgcaccgcat gcgctaaaac gttttgatat gctgtcaacg ataattgtcc
     2041 tatcgtactc cccgaactcc atggctgata catgcttaag cccgtcaaaa gtgaattctt
     2101 tataaacctc atcggatata agatataaat catgttcctt tgctatttct acaagagtct
     2161 gaacttcgtg ccttgtataa acagcgccgg tcggattaga tggatttgaa aaaagtatgg
     2221 ctcttgtttt ggaagttatt ttgctctcta tttcatgttt tgaaggaagg gcgtatccat
     2281 cctctgcttt tgatctggcg gctgtaattt tgatattcaa catttcacaa aaacctctgt
     2341 aattggcata aaaaggttct attgtaagaa tttcatctcc aggatcggca actacagaca
     2401 atgcaaaaac aatagcctca ctgcctccgt ttgtgacaat tatttcttct gctgaaaagt
     2461 ccatattcca ttttttatag tattgagaga acgcttgcct taactccatc agtcccgctg
     2521 aatgtgagta agccagaact tttggggtaa attttttaac agcatcataa aactgttgtg
     2581 gagtcggaag atccggctgt ccaatgttaa gatggtacac tttcttacct ttgctttttg
     2641 cctcatctga aaatggaata agcttcctta taggagaagc ctgcatctgt aaagctcttt
     2701 ttgaaaagtt cattaaaata cctccaccgc atatgaaatt tttttcatat aaatgatact
     2761 attttcatat tttaatatca aagtctaaaa agtacggtta tagacagcaa ttagcgaata
     2821 ttaaagatag acatcaaata attagctaaa ttaattaata tttatctgag ttagccggaa
     2881 atgtattcta atattaaatt ttcgtaattt ctaaaatttt tttttcagtt ttttcacaat
     2941 aaaaagcttt attttcagtt gttataatga ctatagatag caattatcaa cagatatgtg
     3001 aaagttaaca caaaaaagag atatgatttc attttgtaaa gtagaggcat gactatattt
     3061 tcggaaaaac cgacggatat ttaaaaatta aacttaatat gttataattt ctttggaggt
     3121 agaccgatgg ttgattttga aaatacggtt tatgttgcaa ttgactttga aacaacagga
     3181 ctcagaccag atcttggtga caggattata gagatagcag ccttccctat atataagggt
     3241 cagataagaa cggactattc tttttattcc ttggtaaatc ctgaggtaac gatacctaaa
     3301 gaggtttcac attatcataa gcttaataat attcatattg cagatgctcc gttgttgagt
     3361 gaagttataa aggattttaa aaattatctt tcggattcga ttattatttc tcataatgct
     3421 gctatggatc ttagatttat ggatgtggca actaaggaag caggtcttct gccaattagc
     3481 aattattatg cagatacgct taagatgtca atgtattttt ttgatgaagg accttataat
     3541 ttaggatatc tgagcaaaaa gcttaatctt gggataaaca gttttcacag agcctgtgat
     3601 gatgccatgg ctacagccag actgttcctt aagcttgtaa aaaaatactc tgtccgtaat
     3661 ttaggtgatt ttctaaaaag atgggaggga taatatgata aaaaactatg tcattgatac
     3721 caatgttttg attcatgatc ccagagctat tttcaaattt caggataaca acatatttat
     3781 tccttttcct gtcattgagg agatagacaa gcttaaaacc agaagagaca aggtaagtga
     3841 gtcagcgagg agtataaata ggatacttga tggattgaga ggcgaaggta atcttaagga
     3901 cggaataaag cttgaaaatg gtggattttt aagggttatg gctgtagaac ctcagaaaag
     3961 catagatatt ccaagatatc tgggaacatc accggatgat tctatactgt actatgccat
     4021 gtatataaaa aaaatggata caaatatttc cacgatattg gtaaccaaag atctgaatct
     4081 ccggataaaa gccgattcaa taggaatagt tgcacaggat tatttaagtg acaaggttga
     4141 aattgataaa ctttacaggg gatatgcctt tcataccgga aagcctttta aaacagatga
     4201 gtacgaggat attcctccag atcttcttga acttgacgaa agtgtttttc ctaatctcta
     4261 tttatggctt aatgagcggc catataggta taacggtcat aagaatgtgt tttcgccggt
     4321 gaatacaagt tacatctcgg aagtattcgg gataaggcct aaaaattatg agcagatatt
     4381 cgcttttgat gcacttttgg atccttctat tcctttggtg agccttgtgg gaatagccgg
     4441 aactgggaaa actcttcttt ccgttgccgc tggtttgaaa atgatacttg aatcaaaatt
     4501 ttataaaaag atgctggttt caaagccggt aatacctatg ggtaaagata taggatatct
     4561 tcctggtagc attgaggata aaatgaaacc ttggcttcag ccggtatacg ataatcttga
     4621 gtttttatgc tctcttaaag gaaaaaaggt tgatgaatat ctttcaaaaa aagatatcat
     4681 agaagtagag gtgctttcat atatacgtgg caggtcgata cctaatcaat atataataat
     4741 agacgaagct cagaatctga ctccacatga aataaaaacg ataataacaa gggcagggga
     4801 aggcaccaag atagtgctaa ctggagatcc attccagatt gataatcctt atcttgacaa
     4861 ctcttcaaac ggcttggttt atgttgcatc aaagttcaaa ggcagtcctc ttgctgcaca
     4921 tgtaagtatg tttaaaggtg aaagatcaga attagcttcc aaagcggcag agattcttta
     4981 atgaaggagt aaaaatgact gatttcaggg aatatgatat ttatgtaaag atagatcagg
     5041 aaaagataca tattgtgacg tatcttcttg aatcacaggc gcatttaatg tctgtaagaa
     5101 acagacagga aaacggactt ctgaaaataa ttgtacccgg tcctctgaaa gatgaagcca
     5161 ttaagctaat aaattcaatg acggaacaag ttgaactgga ggtagtaaaa gttgagccac
     5221 acaacggggt tgtttaaaag aatccccgtt cctaatgaaa agtgccggga tatttattgc
     5281 attgattata aaactctccg tattcttggt tttaagacag ttctttttga ttacgacttc
     5341 acaataacag gctggaacca gaaaaaaata acagaaaaga cgctcgatct gttcgagcgt
     5401 cttttggata tgggatatca ggtggccata gtgaccaatg ctccttatga cagagtcgga
     5461 catgtctatg aaagtaccca aggtagagta aaagtatatg cagatatgcg taaacctaga
     5521 actgcaaaac tttctaatgt tcttaatctc atgaacacaa cagccaagga aagtataatt
     5581 gtcggcgatc ttttttttac ggatattatg gcaggtaata ttctgggtct gtatactgtt
     5641 cttataaatc cttatacata tggtattgac agtaaggtta aaaaaatggt tgcttttatg
     5701 tcaagagtct tttactatct gtttttctat acagtaggat ggatgttcag aataatggat
     5761 ctggccgtac caaatgagtt taaaaaaagc atacgtgata tagactacac tatgcttttg
     5821 gagtacggat atgatacagt actttttgat tttgataata ctcttatgcc atggcattct
     5881 aacgagatag acgcagaaac tttacaattt ttaagggctc ttttaaatat ggggtttaaa
     5941 ataatgatag catctaacgg taaggatgaa agattttctg acgtaagact tcagctcaaa
     6001 aatatgagga tagaagtcaa cggtatgagt atgaagcctt tggcctgcaa gctaaaaaga
     6061 aaaatttcgc aggccggtta ttccagggat aaaactgtgc ttatagggga tcaacttttc
     6121 actgatgtta tagcaggaaa tctctgcgga ttttatacgg tgaaagtgaa tccgttaagc
     6181 agcagagaag gttttcatac caagtttttc cgcatatttg aaaagctggt tataaagtct
     6241 atgagacgta aacccaatct tatcagaggt gaacagatat gagcaaatgc agcggctgcg
     6301 gcattgaaat acagaatcaa aaccaggata aagccggtta tattccattt tctgtgatga
     6361 cagaaagaat ggccgcagga gatgaagtat tatgtcagcg atgctatagg atcaagcact
     6421 acaataagtt ccttcctgtg ggaatgggaa cggattttta tcagaaacta gaagatattc
     6481 ttcaaacctt taatactata gtatgggtga tagatattat tgactttgaa ggaactttcc
     6541 gcactgatat tgcacttaaa ctaaaaaaca agaatcttat aatagctgta aataaagccg
     6601 atcttctgcc taatgccgta aaatatgaag agataaagga atgggtctgc gatagagtaa
     6661 gccaggttat aagagtaaaa agggataaca taagaataat aagtaccaga agcggttttg
     6721 gaataaacag actctcaaaa atgatatcag aaattaacga tactaaagtt gctgtaatag
     6781 gagtaacgaa tgtcggaaaa tcgtcagtcc ttaatgagat aacagatgct ggggttaccg
     6841 taagctccta tccaggaacc actcttaact ttatcaaaac aaggcttaaa gattcaaata
     6901 ttgaactgta tgacacgcct gggatagaac cgaaagacag agtatgtgat ttttttgata
     6961 tatttactca agtgaaaatg attcctgcta aagagataag cagaaaaaca tttaggttgc
     7021 aggcagggag agtgatgata gtaagtgcat tatgttactt taaggtctta ccggtatcag
     7081 aaagtatgta tgatcctgtt tttctggtct atgccccgca aaatgtttcg tttcatgaaa
     7141 ctaaagaaga aaggataaaa gatattcttg aaaacagaaa agtaattgta ccgccgtatg
     7201 acagttcttt tagtatggag ggtatagtat ttgaaaaaca tacctatgaa gtaaatgaag
     7261 gtgaagatat tgcgataccg ggacttggat ggatttctgt gaggagaggc ccgcttgtga
     7321 tggaactctc tttgccccaa ggtgttactc cggttgtaag aagctcgtta attaaaccgc
     7381 ataagtgatt atattcagga ggtgtaacaa tggttttaaa agaaccagga ataatcgtag
     7441 gagtttcaaa cagacatctg catctttctc agcatgatct tgaagtgctt tttggagcag
     7501 ggcatacgct tacgccaacc aagccacttg gtcagccggg acagtttgcc tgcgaggaaa
     7561 cagtcagttt ggtaggacca aagggcagca ttgagggagt aagagttctt ggacctgtaa
     7621 gaaaggatac tcaggtcgaa gtatcgaaaa ctgattcctt caagcttgga gttaatcctc
     7681 ctgtaagaga ttcgggagat gttgctggtt cacctggttt aaagcttgtt ggccccgtgg
     7741 gggaagtaga acttaagcag ggtgtaatta tcgctaaaag gcatattcat atgtttacgg
     7801 atgatgcggt tcattatggt gtaaaagata aagattatgt ttctgtaatg tgtgaaacac
     7861 acgatagaaa attaatattc caggatgttc tcataagagt aagcgaaaag tttgcacttg
     7921 aatttcatgt tgatatagat gaagcaaacg cagcccttct taaaacggga gataaaataa
     7981 agataataga gaagttctaa ttgtccggaa ccatgaaggt tccggatttt ttaccttaag
     8041 gtttcagaat gaataaattg gtgagtatct tgaaggaaga gtcttgacta atacagatta
     8101 atgtagtata ataactttgg ttaaatcgtg caggcatagc ttcaatcggc agagcgctgc
     8161 cttggtaagg cagaggttat gggttcgatt cccattgcct gctccatttt ctgcggatgt
     8221 agctcagtgg tagagcacct gcttgccaag cagggggtcg cgggttcgaa tcccgtcttc
     8281 cgctccagaa ctcccatact gggagttttt ttttatttaa aggtcttagt atcagtatat
     8341 taaaagactt atcatggtaa aatactaata agtaggtgta ttgagaggct gagaccatgg
     8401 atagagaagt ttacagactg agaaggctta atctggacag gcttgtaaaa ttggcacagg
     8461 taggggataa taaagctatg gggatggttc tggtaaaact cgagcctatg gtaaaatcaa
     8521 taatctctaa gtattacggt acctgggcag attttgagga ctttcttcag gtaggtttcg
     8581 tcggtgtaat gcaggcagtt tacagttata atgactcctc taatactaaa tttaccactt
     8641 ttgcatatct taatatttcc tcggagataa agtcttttat aacatatctt
//
LOCUS       DAOSHR010000082         8582 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_7020_length_8582_cov_5.783863, whole genome shotgun sequence.
ACCESSION   DAOSHR010000082 DAOSHR010000000
VERSION     DAOSHR010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 8582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8582
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7020_length_8582_cov_5.783863"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="PLU28_05795"
     CDS             complement(<1..421)
                     /locus_tag="PLU28_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOG34612.1"
                     /translation="MKPKKKTALMIALIIIGVMMALPFFWMLITSVKPDTELFSWPPS
                     FIPKNITLEHYAEVFHTKDFGRYFVNSLIVACLSVIMNLFFCSLAGYAFARLDFKGKN
                     FAFIVLLSTMMIPIQVTLIPTFILVKSFPLAGGNNIFG"
     gene            complement(418..1329)
                     /locus_tag="PLU28_05800"
     CDS             complement(418..1329)
                     /locus_tag="PLU28_05800"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOG34613.1"
                     /translation="MKNQTRKNGQLSSMKKKIAFTAVIFIAPVFIYTVIFRIIPIFFS
                     LILSFTRYDGFNAPVFNGIQNYKALMFSGEFWNSVIRTLEFSVLVLPLNMLISLVMAV
                     LINNRVRGIGFFRALYYLPVITPLVAASMIWMWLYDPQYGIFNFFLSLFSLPPVNFLS
                     NTSTALASVAVMRIWRGVGWNMMIYLAGLQGISAYLYDAAKLDGAGTVKQFTKITWPL
                     LRPVHLYVLIVGLISTLQSFTEMYVMTGGGPLQSTTTVGMLIYTSAFDYMDMGYACSM
                     SFALGIIIMVLSVISFTWKKERTEVSE"
     gene            complement(1407..2639)
                     /locus_tag="PLU28_05805"
     CDS             complement(1407..2639)
                     /locus_tag="PLU28_05805"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOG34614.1"
                     /translation="MKKLILTVLLCTAVFMGVFGANTVKVWFAGTQKQLMDLVDSKLI
                     PEFEKNNPDIKIKAEFIPWGELSTKLTTSFAGGIGPDIFMHGQAAIAGFADKGIIMPI
                     DSILSKIEDPQDFGATLNAGLFKGKKYYIPVFGSGRLLAYRKDFFVQAGLDPQDAPMT
                     WEQLISYSKKLTSVENNRYKCAGFDLPVSGVDLQQVWSGFLYQNGGDLFDNEMNPVFN
                     STQGVQALQFYVDLIRKEKVAPDYGTSKTGNLHSLASGSAAMGMLVLEEINDIKTYAP
                     QAFEQIELSLSTARLNRASFYSFAGFMVSSKTKDLQSTTKVLSYLTSKNSIVAINEVQ
                     STLPPRQSAAESEFVSSNPVLTMFVKGAKYAFGNPNVPFWVQARDILIKYLERAVKGN
                     MDCKSALDQAAQEIMKLK"
     gene            complement(2645..3337)
                     /locus_tag="PLU28_05810"
     CDS             complement(2645..3337)
                     /locus_tag="PLU28_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34615.1"
                     /translation="MKSFKELLDTEKKLLIVSLPDNSCEYALRAYDAGADAVKFHVNI
                     KHRVTQNTYNTWKDFKKTFEYIISQIDIPAGIVPGAEIMSSIEDFKDMERSGVTFIDC
                     YYEYAPRWIKTLSVHKMLAVNHTFKEEELDYLVDYGADSIELSFIDPSQYGKALTVKD
                     CINYERLSKKCHSNCFIPSQKFLTIDDLEVLNNQNIKGIILGAVVTGSDPKTFSDTIK
                     NFRDGLDKSYGR"
     gene            complement(3353..4342)
                     /locus_tag="PLU28_05815"
     CDS             complement(3353..4342)
                     /locus_tag="PLU28_05815"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOG34616.1"
                     /translation="MKPTIVDIAKELNLSPSTVSRALKNNPRISIQTKNKVIQTAKKM
                     NYLHEEDISICNRIILIIIANPQKSVENDEFFSVFLQGALEGAKKESLHCLIQSIRSK
                     ESYKRALLPVSKVNGIILCGIPMPEELISFIKKLDIPCVQIGNYKGLEAFTSINNDNH
                     RGGQLVGKAVLDKGYKNISIITGSLSLKTFKDRLDGFISVVQNDETVSYTIYECSDFD
                     EKSGYGKTMELYKNSTLKEKHCIFCTTDWLAKGALNSLEEMKVAVPESTGVIGYGNLS
                     FSSYLKPPLTTVALNPYLLGRLGITMLNEKMDCNPNLSSTVFIEPIITLRQSL"
     gene            complement(4536..4611)
                     /locus_tag="PLU28_05820"
     tRNA            complement(4536..4611)
                     /locus_tag="PLU28_05820"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4576..4578),aa:Pro,seq:cgg)
     gene            complement(4655..6031)
                     /locus_tag="PLU28_05825"
     CDS             complement(4655..6031)
                     /locus_tag="PLU28_05825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOG34617.1"
                     /translation="MQSNGKVDILNSSIGKALFKLAWPLVLTNLLQTMYNITDAFFLG
                     KLGTAEFSAPTLAQPIMFVFIALGAGFSQAGSTIIAQYTGMKRQDLAEKNAAQAILTT
                     LVLSSVFSLVGILSARAIVSQMALSKYTIDLAVTYMVIELASVPFMFVMQLNSGILRG
                     WGNSVIALRLTFVSVMMNVVLDPIFIFGFKQGVAGAAIATFISRFVIAVYFTYLMFSG
                     KYGFKLRVKDFKPDFSSIMQVIKIGLPASFGQAVTSLGFTLIMGVVSRFGVEVVSAYG
                     VGQKISQLMVNFASGVSLACASMIGQFLGADRPDLASRTVKKAAIFTFSVALAVSTLL
                     FFFGHNVTQFFVNDPKVIEMGEIYFRLISFSLPFFTTLAVFMDTLRGSGHTVQSTIID
                     IVRLWGIRIPIVTILAEKYGFRGVFYGMIISNISAMFLAMAFLVFGNWKKKIVKVQKI
                     SGEHVEEG"
     gene            6195..7367
                     /locus_tag="PLU28_05830"
     CDS             6195..7367
                     /locus_tag="PLU28_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34618.1"
                     /translation="MKYIQKIIELVANLGTPSFHNLLGNLIVSETHSSKMIIVRFFEN
                     LWVVLDAKNVFPSKLEYQNIEDIIREAGLEKIYDFPFDTYILCDGKFEDEETSRMIVQ
                     AFEKEKELFENQRLLTVIDRLSYQLSSIESLVLNLSEPLSIEKFMEIVASGISELLFC
                     SVSIYRVNERKALKTEEYGRLKLNIDEFELSEELMHSIEISSPIIINRYNKFQDKAFD
                     KIKAVIPVGTFENDTFLLFITRDTSIEEEEKLFINTIYRIIFHFYTKQFLEILDFKEE
                     IAAKTLNILKVFDNIIYMIKGKDNIEERFFQSIGSMTFVEQSQVFSPQKALADGVYSS
                     RECSGTEYDLVVSYTSPQGIKKSIGITVKKSFKNSILEIINIVSLVLDGYETIIEN"
     gene            complement(7395..7664)
                     /locus_tag="PLU28_05835"
     CDS             complement(7395..7664)
                     /locus_tag="PLU28_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HOG34619.1"
                     /translation="MAEAEVLKVAANSKPIAVAGALSAIIRDHGIAELQAIGAGAVNQ
                     AVKAIAIARGYVAPSGIDLVCIPAFSDVNIEGEERTAIKFTIKNR"
     gene            complement(7719..7795)
                     /locus_tag="PLU28_05840"
     tRNA            complement(7719..7795)
                     /locus_tag="PLU28_05840"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7759..7761),aa:Arg,seq:tcg)
     gene            7935..>8582
                     /locus_tag="PLU28_05845"
     CDS             7935..>8582
                     /locus_tag="PLU28_05845"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HOG34620.1"
                     /translation="MTDLKVTAYENYKKWVENADLSLKDDLEKIRSNEKEIVECFYKD
                     LEFGTGGMRGKIGVGSNRMNIHTVARASQGFADFLKTQNQNLSVVIAYDTRNKSELFS
                     KVSAEVFAANGLKVFLFSKPTATPILSYAVRHLHAAGGLVITASHNPKEYNGYKVYTS
                     DGTQAVPVYADIITRNVESLDHFKDVKRSDYEELIKSGMIQMLDESVFNSYIETVF"
BASE COUNT         2691 a         1642 c         1650 g         2599 t
ORIGIN      
        1 gcccgaagat attgtttcct cctgccagag gaaatgattt taccaggatg aaagtcggta
       61 tcagtgtaac ctgaatcggt atcatcatag tactcaaaag gactatgaag gcaaagtttt
      121 ttcccttgaa atcgagtctg gcaaaggcat aacctgcaag actacagaaa aaaagattca
      181 tgattactga caggcatgcc acaataaggc tgttgacaaa atacctgccg aaatctttgg
      241 tgtggaatac ttctgcataa tgctccaaag taatgttttt cgggataaaa gacggaggcc
      301 atgaaaacag ttcagtatca ggtttgacag atgtgataag catccagaaa aatggaaggg
      361 ccatcataac gccaattatt atgagtgcta tcatgagagc tgtttttttc ttaggtttca
      421 ttctgacacc tccgttcttt cttttttcca cgtaaagctt ataaccgata gtaccatgat
      481 gattataccc agcgcaaagg acatagaaca cgcatacccc atatccatgt agtcaaaagc
      541 acttgtatat ataagcattc caactgttgt tgtcgattgc agcggaccac ctcctgtcat
      601 tacgtacatt tctgtgaaag actgcagggt gcttatgagt ccgacaatca gaacataaag
      661 atgaaccggt cttagcagag gccaagttat ttttgtaaac tgtttaacag tacctgcgcc
      721 gtctagcttg gcagcgtcat aaagatatgc agatattccc tgaagtccgg caagatagat
      781 catcatgttc cagccaacac ctctccatat tctcattacc gcaacgcttg ccagtgccgt
      841 tgatgtattt gaaagaaagt taacaggcgg cagactaaaa agcgaaagaa aaaaattaaa
      901 aatgccatac tgcggatcat aaagccacat ccatatcata gaagcagcaa caagaggagt
      961 gatgacaggc agataatata aggctctaaa gaatcctatt ccgcgaaccc ggttgtttat
     1021 aagtacagcc atgactaagg atataagcat attcaggggt agtaccagca ctgaaaactc
     1081 caaagttctg atgaccgaat tccagaattc tccactgaac atcagagctt tatagttttg
     1141 tataccatta aaaacaggtg cgttaaaccc atcgtagcgt gtaaagctta atataagcga
     1201 gaaaaaaatt ggtattattc tgaatataac ggtataaatg aatactggag caatgaaaat
     1261 aacggctgta aaagcgatct tttttttcat tgaagaaagc tgtccgtttt tccgggtttg
     1321 atttttcaag attttcctcc tacagtaaag taatctgctg tttttttgat aaaagaccgg
     1381 cgtatgccgc cggtcatctg tataaattac ttcaatttca taatttcctg ggcagcctga
     1441 tccaatgctg atttgcaatc catattgcct tttactgctc tttcaagata ctttatgagt
     1501 atatcccttg cctgaaccca gaaaggtacg ttaggattgc caaaagcata tttggctcct
     1561 ttcacaaaca tagtaagaac aggatttgaa gaaacaaact ctgattcagc tgccgactgt
     1621 cttggaggca gggtggactg tacttcgttt atagcaacta tagaattttt ggaagtcaga
     1681 taacttaaaa ccttggtagt gctttgaaga tctttggttt tgcttgatac cataaagcct
     1741 gcaaaagaat aaaagcttgc cctgttaagt cttgccgtgg acaatgaaag ctcaatctgt
     1801 tcaaatgcct gtggagcata agttttaata tcgttaattt cttcaagtac cagcatgccc
     1861 attgcggctg aaccgcttgc cagtgaatgt agattgcctg tttttgaggt tccgtaatca
     1921 ggggcaacct tttcttttct tattaggtca acgtaaaact ggagtgcctg tactccctga
     1981 gtactgttga aaaccgggtt catttcattg tcaaaaaggt ctccaccatt ttgatataag
     2041 aaaccgctcc agacttgctg aagatcaaca ccgcttacag gaaggtcaaa gccggcgcat
     2101 ttatatctgt tgttttcgac tgatgtaagt ttttttgaat aactgataag ctgttcccat
     2161 gtcatcggag catcttgagg atccaaccct gcctgaacaa aaaaatcttt tcggtatgct
     2221 aaaagtcgtc cgcttccgaa aaccggtatg tagtattttt ttcccttaaa caaaccagca
     2281 ttaagagtag ctccgaagtc ctgaggatct tctattttgg atagtatcga atcaataggc
     2341 attattatgc ctttgtcagc gaaaccggct atggcagcct gaccatgcat aaatatatcc
     2401 ggtcctatcc ctccggcaaa ggatgtagta agtttggtcg agagttctcc ccacggaata
     2461 aactcagctt ttatctttat atccgggttg tttttttcaa attccggaat aagttttgaa
     2521 tctaccaggt ccattaactg tttctgagta cctgcaaacc aaacctttac tgtattagcc
     2581 ccaaagacac ccataaaaac tgcggtacat aataaaaccg ttaaaataag ctttttcata
     2641 tatcctacct cccgtatgat ttatccaaac catctctgaa atttttgatc gtatccgaaa
     2701 aagtttttgg atcgcttccc gttacaactg ctcccaggat aattcccttt atattttggt
     2761 tgttaagtac ttcaagatca tctatggtta ggaacttctg agaaggaata aaacagtttg
     2821 aatggcattt ttttgaaagt ctttcatagt ttatgcagtc ttttacagtc agagcctttc
     2881 cgtactgtga tggatcaatg aaagaaagtt caatgctgtc tgccccatag tcaacaagat
     2941 aatcaagctc ctcttcttta aaagtatggt ttacagcgag catcttgtgg acggataagg
     3001 tcttaatcca acgtggcgca tattcgtagt aacagtctat aaaagttact ccggaacgtt
     3061 ccatatcttt aaaatcttct atactgctca ttatttcagc tccgggtact ataccggcag
     3121 gtatatctat ttgagatatt atgtattcaa aggttttttt gaaatctttc catgtattgt
     3181 aagtattttg agtaaccctg tgttttatgt ttacatggaa cttaactgca tcagcgccgg
     3241 catcgtatgc ccttaatgcg tattcgcatg agttatccgg cagacttaca attaacagct
     3301 ttttttctgt gtctaacaat tctttaaagc ttttcaatca tctcatctcc tgtcatagtg
     3361 actgtcttag agttattatg ggttctataa atactgttga agaaaggttg ggattacagt
     3421 ccattttttc attcaacatg gttattccga gccttccaag aagatatgga tttagcgcaa
     3481 cagttgtcag gggaggctta aggtacgacg aaaagctgag attgccgtac cctatgactc
     3541 ctgtactttc aggaaccgct actttcattt cttcaaggga gtttaacgca ccttttgcca
     3601 gccagtcggt ggtacaaaag atacaatgtt tttcttttaa agttgagttt ttataaagct
     3661 ccatggtttt tccatatccg gatttttcat caaaatcact gcattcatag attgtataag
     3721 atacagtttc gtcattttgc acaacggata taaaaccgtc gagcctatcc ttaaatgttt
     3781 ttagtgagag agagcctgtt atgatggata tgtttttgta tcctttgtct aaaactgctt
     3841 ttccgactaa ctgaccgcct ctgtggttat cgttgttaat ggatgtaaaa gcttcaagac
     3901 ctttataatt accaatttga acacacggaa tatcgagttt ttttataaag cttataagtt
     3961 cttcaggcat cggaattccg cagagtatta ttccattcac ttttgatact ggaagaagtg
     4021 cacgcttata gctttctttt gatctgatgc tttgtatcag acaatgaaga ctttcttttt
     4081 ttgccccctc gagtgcccct tgaaggaaaa cagagaagaa ctcatcattt tcaacagatt
     4141 tttgaggatt tgcaataatg ataagtataa ttctgttgca aatggaaata tcttcttcat
     4201 gcaaataatt catttttttt gctgtctgaa taactttatt tttggtttgt atacttattc
     4261 tcggattgtt ttttaatgct cttgacactg ttgaagggga taaattgagt tcctttgcaa
     4321 tatcaactat agtaggtttc atcagcatcc tcctgtgcaa taatgtgcaa atgtacttat
     4381 aaagccgcct tattctagca ttaaaaaaaa ggtaaatcaa aacagaaaat tgatgaaaat
     4441 caaagaaaaa ataaaataac tgctttccga tgtaccggaa agcagttatt tgagattaaa
     4501 tgaaaaaatt ctgctttact aaaagcagaa ttttttggtc ggggcgactg gatttgaacc
     4561 agcggccttc agttcccgaa actgacgcgc tcccacctgc gctacgcccc gatgaaaagc
     4621 acaatgatta ttgtaacata ttccagagaa aaaatcaacc ctcttctaca tgttctccac
     4681 ttattttttg aaccttaaca atcttctttt tccagtttcc gaaaaccaga aaggccatag
     4741 ccaggaacat cgcagatata ttacttataa tcatgccata aaaaacaccc ctgaaaccgt
     4801 acttttcggc taaaatagtt actataggga tccgtatccc ccaaagtctt actatatcta
     4861 ttatagttga ctgaacagta tgtcctgaac ctctcaaagt atccataaat actgctaagg
     4921 ttgtgaaaaa aggcagtgaa aaagagatca gcctgaagta aatctcaccc atttcaatta
     4981 cctttgggtc gtttacaaaa aattgagtaa cgttatgtcc gaaaaagaaa agcagggtac
     5041 ttactgccaa tgcaacagaa aatgtaaata tagctgcttt tttgactgta cgtgaagcca
     5101 aatcaggtct atccgctccg agaaactgtc cgatcattga agcacatgcc agagatactc
     5161 ctgacgcaaa gttaaccatg agctgactta tcttctggcc gactccgtaa gcacttacga
     5221 cttcaacacc aaatctggaa actacaccca ttataagggt aaatccaagc gaggtaactg
     5281 cctggccaaa cgaagcagga agtcctattt ttattacctg catgattgaa ctgaaatctg
     5341 gcttgaaatc ctttactctg agtttaaaac cgtactttcc actgaacata agatatgtga
     5401 agtatacggc tataacaaac cgtgaaatga aggttgcaat agcggctcct gcaactccct
     5461 gtttgaatcc gaatataaaa ataggatcaa gaaccacgtt catcattacc gatacaaagg
     5521 tgagcctcaa agctattacg gagtttcccc agcctctgag aattcctgaa ttcagctgca
     5581 ttacaaacat gaaaggaact gaagctaact ctattaccat atatgtgacc gcaaggtcta
     5641 tggtgtattt tgataaagcc atttgggata ctatggctct tgcactgagt attcctacaa
     5701 gtgaaaaaac cgaggaaaga acgagagtag taaggattgc ctgagcagca tttttttctg
     5761 ccagatcctg tcgtttcatt ccggtatact gagcgattat cgttgaccct gcctgtgaga
     5821 agcctgctcc caaagcaata aaaacaaaca ttatgggctg agctagcgtt ggagcagaaa
     5881 actcagcggt accaagcttt cctaagaaaa aagcatcagt aatgttgtac atagtctgaa
     5941 gaagattagt taaaactaaa ggccacgcca atttaaaaag ggcctttccg atagaagaat
     6001 taagtatatc aacttttccg ttggattgca cgagtagtcc tccttaacat tttataaaaa
     6061 aactttataa ttaaaaaata aatataacaa tctttatgta tgctgtgctt actattatac
     6121 catattttaa atgagtaata aatgtgttat aataaatcag tacggagttt aagaaaaaaa
     6181 gccgggggta gtgtatgaag tatatacaga agataataga actcgttgca aatcttggaa
     6241 ctccatcctt tcacaatctt ttaggtaatc ttattgtttc agaaacacac agctcgaaga
     6301 tgataatcgt ccgttttttt gaaaaccttt gggtggttct tgatgcaaaa aatgtttttc
     6361 cttccaagct tgaataccag aatatagagg atatcataag ggaagccgga cttgaaaaga
     6421 tatatgactt tcctttcgat acgtatatac tgtgtgacgg caagtttgaa gatgaggaaa
     6481 catcaaggat gattgtacaa gcctttgaaa aagagaagga attattcgaa aaccaaaggc
     6541 ttttaacagt tattgacagg ttgtcatatc agctttcttc catcgaaagc ttggttttga
     6601 atctttcaga accgctcagc atagaaaagt ttatggagat agtggcatca ggaatatctg
     6661 agcttttatt ctgcagtgtt tctatatata gagttaatga aagaaaagct ttaaaaacag
     6721 aggagtatgg aagactaaaa ctcaatatcg atgagtttga actttcagaa gagcttatgc
     6781 acagcattga gatcagttct cctattatta taaacagata taataaattt caagataaag
     6841 cttttgataa aatcaaggcc gttattcccg ttggaacatt tgaaaacgac acatttctat
     6901 tgttcataac cagggatacg tctattgagg aagaggagaa actctttata aatactatat
     6961 acaggataat tttccatttt tataccaaac agtttcttga aattcttgac tttaaagaag
     7021 agatagccgc gaaaacactt aatattttaa aagtttttga taatattatt tatatgataa
     7081 agggtaagga taacattgaa gaaagattct ttcagtctat aggcagcatg acttttgtag
     7141 aacagtcgca ggtgttttcg ccgcaaaagg ctcttgccga cggagtctac tcatccagag
     7201 agtgcagcgg tacggaatac gatctggtag tatcttacac gagccctcaa gggataaaaa
     7261 aaagtatcgg aataaccgtg aaaaagagtt ttaagaacag catacttgaa ataattaata
     7321 tagtaagtct tgtattggac ggatatgaaa ctataataga aaattaatcg gagccctagg
     7381 ctccgattaa ataattacct gttttttatg gtgaacttaa tggccgttct ttcttcaccc
     7441 tcaatattaa catcggagaa ggccggaata caaaccagat caattccgct gggcgcaacg
     7501 taacctcttg ctatggctat agcctttacc gcctggttga cagcaccggc tccgatagct
     7561 tgcagttcgg ctataccgtg atctctgatt attgcagaaa gagctccggc gacagctata
     7621 ggtttggagt ttgcagctac ttttaaaact tcagcttctg ccatagttat agcctccttt
     7681 tttataatac aacttcaatt ttggtatttg ctttataatg gcgcgcccgg cgagattcga
     7741 acccacaacc ttcggatccg aagtccgacg ctctatccag ttgagctacg ggcgctctat
     7801 aaaactatta taccttaatt agtcaataaa atcaacaata ggtttgggtt attggcagac
     7861 aattggaaga tctttggcag tatggtataa tttgattgta atcagcatct ttgtttataa
     7921 ggggagggaa tacaatgaca gatttaaaag tgactgctta tgaaaattat aaaaaatggg
     7981 ttgaaaatgc agatctgtcc cttaaggatg accttgaaaa gataagaagc aatgaaaaag
     8041 agatagtgga gtgtttttac aaagatcttg agttcggaac aggaggaatg agagggaaga
     8101 tcggagtagg gtccaacagg atgaatattc atactgttgc cagggctagt caagggtttg
     8161 cagatttttt aaagacccaa aatcaaaatc tgtcagtggt aatagcctac gataccagaa
     8221 acaagtcaga gcttttttcc aaggtatcag ctgaggtctt tgcagccaac gggttgaagg
     8281 ttttcctgtt ttcaaagcct actgcaacac caatcctttc atacgctgtg agacatcttc
     8341 atgcagcagg cgggcttgtc ataaccgcaa gtcacaatcc taaagaatat aatggataca
     8401 aagtatatac atcagacgga acacaggcag ttcctgtcta tgcagatata ataaccagaa
     8461 atgttgaaag tcttgaccat tttaaagatg tcaagcggtc tgattatgaa gaacttataa
     8521 agtccggtat gatacagatg cttgatgaaa gtgtttttaa cagttatatt gagactgttt
     8581 tt
//
LOCUS       DAOSHR010000083         8515 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_7117_length_8515_cov_5.560402, whole genome shotgun sequence.
ACCESSION   DAOSHR010000083 DAOSHR010000000
VERSION     DAOSHR010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 8515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8515
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7117_length_8515_cov_5.560402"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(179..1120)
                     /locus_tag="PLU28_05850"
     CDS             complement(179..1120)
                     /locus_tag="PLU28_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOG34621.1"
                     /translation="MQNDVEKLVEMLSKVFSNTKIKEIMTRPPIVVTQETKIKYVKDI
                     MRIKGISGMPVIAPGFKLVGIISIEDIIRVFENGSFDDRVNKWMTPDPVYLKEDEMLL
                     HFLQVNEDLKYGRYPVVNNDHKVVGVVTKLDILSWMFKKLGYIYVHDERREKALNKKY
                     MSTITGELLDKEGVDFSYKIDYDSVEMIGAAATRLKKFLKEKKIQEDIIRKISIATYE
                     AEANVVIHSSSTGNVYCWIKQDHIKVLIEDFGKGIEDLEQAMKEGFTTAPDNIKEKGF
                     GAGMGLPNMKRFSDRMTIVSSIGKGVKVEILYYIKEK"
     gene            complement(1120..1503)
                     /locus_tag="PLU28_05855"
     CDS             complement(1120..1503)
                     /locus_tag="PLU28_05855"
                     /inference="COORDINATES: protein motif:HMM:NF018753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HOG34622.1"
                     /translation="MKILQILKLLNAEAVFMPPGSDEYEIEYSGAADLMSDFLAFSRE
                     HMLLITGLTSPQTITTAAVIGANGVIFVRGKEIPPEVLKTAQELDIPILKTSISMYIA
                     CAILYKNGIKDAMGTDRFLDNGDTL"
     gene            complement(1510..2211)
                     /gene="thyX"
                     /locus_tag="PLU28_05860"
     CDS             complement(1510..2211)
                     /gene="thyX"
                     /locus_tag="PLU28_05860"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HOG34623.1"
                     /translation="MQEIKCSILDKGFVTLIDMMGDDFSAVKAARVSYAKEQSSDIER
                     DRALIEYLMKNKHESPFEHITLTFHVKCPIFVARQWLRHRIGNSYNEISRRYTTKYSE
                     EFYLPDHIRVQDEENRQSSYICEDDNKKEQALLLIEESYKNAYETYQKLLKLGIAREM
                     ARIVLPVGEYTQFYWTSNIRSIMHFLNLRADGHAQYEIQLYAKAIAEIFSKVCPLSYT
                     AFLKYSYTGNILKEV"
     gene            complement(2227..3375)
                     /locus_tag="PLU28_05865"
     CDS             complement(2227..3375)
                     /locus_tag="PLU28_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34624.1"
                     /translation="MKKLTVAFFLVFLTVIAVFASSLDRYVFVFDDEKLVSDSKELSV
                     LGFYLKYWKSGYEEYKSTANTIFSENMDLFFPDDTAVLDIFSSINPQEFLSPGKKFRS
                     AKETYPQSIIINAFFIEFLYSDWERSGEIQVGQEIIDSCNNLEQLAGTNPLSSFYKAK
                     VLWKSKVIGDSSQAIQIIKDIYTEYSDNIKILYEYIVMSYQNEDYKKATDLYENYIKL
                     GESADEIHLIAAKSYLNLENKDKARELASSITKSSGRKNILASTYELLGDIAQTNVQK
                     INYYKLSLEYDEKNGLIWSKLGVSYLQEDRKENAQLARVILNKALSLYPNQPEIRAEL
                     DNLQRDIKIKGVMVYILPLLGIVIGGITVMIVLEKRKKNKEYEEMLKK"
     gene            complement(3388..4758)
                     /locus_tag="PLU28_05870"
     CDS             complement(3388..4758)
                     /locus_tag="PLU28_05870"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HOG34625.1"
                     /translation="MAKLKLKVSGDSMVASVSLLDDGVYLKVDDVLAFLQSNGISYGI
                     SMETIRELCANVKFNSEIPVAYGKGPKVGDDGKVNILVRREKVNYDTEKKVDLREVLN
                     FLIVEANEKLAQILPPAKGENGKDVYGKEIEGLIGKEAKCTVGKNAFIQDGYVIAKVS
                     GELVFKREANDALNIDVSEVKRIEGDIDYNIGNVRFPGSLVINGNVRPGFIVEAEQSI
                     EIKGIVESATVIAGGEITVSGIKGAGKGIIRADTVKAAFIENAEVEAKKNIYVKQAII
                     NSKVKAGETVVVIGKNGRIIGGNIIAGNKIEADFLGSEMTVTTNLEVGVQPELNEELT
                     ILSSQIAIDLENLRKLSLILKGMVKLQNEGRLDQEKIEQYKRSLQTAKTLKDNLESNE
                     KRLEEIKKIIESSENDGFILAKECVYPGASITIHKRRFFPDRAMVKVMFMSIEDKIVL
                     KGYSKD"
     gene            complement(4775..5179)
                     /locus_tag="PLU28_05875"
     CDS             complement(4775..5179)
                     /locus_tag="PLU28_05875"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG34626.1"
                     /translation="MSQIRVEIESKSSYIKALRQVLKEFLRVNNYNEEDEIFMFELAL
                     NEAIANVIEHTYNFEENHSINVEFSIDEERVFCIKIRDFGRKVDPQKIQPRDLEDYRD
                     GGLGVFIIQKVFGNMRWDDVPEGNLLYLYKKL"
     gene            complement(5195..5995)
                     /locus_tag="PLU28_05880"
     CDS             complement(5195..5995)
                     /locus_tag="PLU28_05880"
                     /inference="COORDINATES: protein motif:HMM:NF014707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HOG34627.1"
                     /translation="MLVHVCCVPDLLAASKILANEDLKVYVYFYNPNIDDSNEYLLRK
                     SQLKKIPLEYGFEEIQSLYDPNRYLTFVSENIAGMDRCQKCIYMRLEKTFETAAENGF
                     LHVTTTLTASPRKEQEFIRRIGEAMAVRYRVAFDFYNFRSDNGISFGADLCRRFGIYR
                     QTFCGCSFSKKESEELHILSQKNRFKELSEIVGELKAGNLFRFNGSAEIRIPYDICLE
                     DIKEDPMKILKSLRPKSIVINSSEAKEIGITKSGRINIGNWRAKFIVV"
     gene            complement(6001..6507)
                     /locus_tag="PLU28_05885"
     CDS             complement(6001..6507)
                     /locus_tag="PLU28_05885"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOG34628.1"
                     /translation="MKKLFFTFLAVFAVTLIGVIAIINQTDNEKETQGVYLVDISSAD
                     IKELMNIPFIGYSKALNIIEYREQHGFSCIDDLKNVTGIGDKTFEKIRNYVTISTKKF
                     EYRQTKVKINSDSIQKLEELEGIGEKTANIILEYRKIKTIENMDELKKITGMSDKQIE
                     KIRGYIEF"
     gene            complement(6513..7247)
                     /locus_tag="PLU28_05890"
     CDS             complement(6513..7247)
                     /locus_tag="PLU28_05890"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOG34629.1"
                     /translation="MKKKYNKSFIFFVIIVFIVPVLTFILTIYTISLKNKIKDLKLSI
                     NDLRSEVVLDSIQSTPTENMQGVPSYSMTEPNAPKINYELKDVNYYNLLAVASMSIES
                     QSGGHARTIDEKEAYLQAVQAYKKEIPFMISKNGDEYVLYSKEFTNNDLNSLYSIQLA
                     TYSNIEGAYVACNLLRKEGVAAYVYKGYYAQSGKEYHALMLGIFTSRKSADTINASLD
                     LDSIYKLIGVDVKGRYIRLMDFEPRT"
     gene            complement(7249..8244)
                     /locus_tag="PLU28_05895"
     CDS             complement(7249..8244)
                     /locus_tag="PLU28_05895"
                     /inference="COORDINATES: protein motif:HMM:NF013887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34630.1"
                     /translation="MRSISFIGNEGKKIEITIGYGQYKKTIKTLSQKYGTYTVADSKI
                     KTMMESKSFIKYVRGGEEAKFIDKYLEVADKIVNNDISTLIGIGGGAVLDLCGYTVNT
                     INCNVKNLILYPTTLNSFIQANISGYFFLDMNYSYNYLKVRGYPDIIYIDPEFLSKIS
                     GHILRKMFFSGYFLGFLFDKNYCKLSMKYAHNFSGLDVEEYIYENLIFLTYLYSSQEI
                     FLPGSNLIKYFYSKPGDFKKEYTVVFSNIFLFLAYISLSNSYISYEQYVSIKKDMIEL
                     ELYNKNIMQYIAFDKIGSVIEEVVPHSQSSFRKVLLHKEELKEVLLEFMQSNRGD"
     gene            complement(8263..>8515)
                     /locus_tag="PLU28_05900"
     CDS             complement(8263..>8515)
                     /locus_tag="PLU28_05900"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34631.1"
                     /translation="LRTLEKAAQEIQDYDPSYLIVSLGTDTHKDDPLGNFMLGDSDFF
                     EIGKILSELDIFKLVIQEGGYNPIANASAVDSFLAGLLI"
BASE COUNT         2515 a         1786 c         1194 g         3020 t
ORIGIN      
        1 aatattctga tgcgtctgat atgtaatcca agctgaatca ggtttcgcag tacgcatcac
       61 ctcactgaga agatccccta tattaccgtt tataatttcg atatcgtcgc tcccagccga
      121 aataatccta agattaaggt cttttattat tttttcaagc ttcattttcc ccctccgatt
      181 atttttcttt tatatagtat aaaatttcaa cttttactcc cttacctata cttgatacta
      241 ttgtcatcct gtcagaaaat cttttcatat taggaagacc cattccagca ccaaaccctt
      301 tttcctttat gttatccgga gctgtggtaa atccttcttt catagcctgt tcaagatctt
      361 cgatcccttt tccgaaatcc tctattaaca cttttatatg atcctgcttt atccaacagt
      421 atacgttacc cgtcgaactg ctgtgaataa caacgttggc ttcagcctca taggttgcaa
      481 ttgatatttt ccttattata tcttcctgta tctttttctc cttaagaaat tttttaaggc
      541 gtgtcgcggc ggctcctatc atttcgaccg aatcgtagtc tattttatat gaaaaatcga
      601 ctccttcttt atcaaggagc tctccggtta tggttgacat atacttctta ttcaaagctt
      661 tttctcttct ttcatcatga acatatatat acccgagctt tttaaacatc cagcttaaaa
      721 tatcaagctt tgtaactact cctactactt tgtggtcatt gtttaccacc gggtatcttc
      781 cgtatttcag atcttcatta acctgaagaa agtgaagaag catttcatct tccttaagat
      841 acacaggatc cggagtcatc catttattta ccctgtcatc aaaacttccg ttttcaaata
      901 ctctgattat atcttctata cttattatac ctaccagctt aaacccaggt gcgataacag
      961 gcattcctga gatccctttt atacgcatta tatccttaac atacttaatc ttggtctcct
     1021 gagtaacaac tataggaggc cttgtcatta tttctttaat ttttgtgttc gaaaatacct
     1081 ttgaaagcat ttcaactaat ttttcaacat cattttgcat tataaagtat ccccattatc
     1141 caaaaacctg tctgttccca tagcatcttt aatgccgttt ttatacaaaa tagcgcatgc
     1201 aatatacatt gaaatacttg tcttgagaat aggaatatca agctcttgag cggtttttaa
     1261 aacttcaggg ggtatttcct ttccacgcac aaaaataacc ccgtttgcac ctatcactgc
     1321 agcggtagtt atggtttgtg gactcgtaag tccggttata agaagcatat gttctcttga
     1381 aaaagcaaga aaatcactca taagatctgc agcaccggaa tactctatct catactcatc
     1441 agaacccgga ggcataaaga cagcttccgc attaagtagt ttcaatatct gaagtatttt
     1501 caagatcggc taaacctcct tgagaatatt acctgtatag gaatacttta agaaggcggt
     1561 ataagaaaga ggacatacct tactaaaaat ttccgctata gcctttgcat acagctggat
     1621 ttcatactgt gcatgtccat ctgccctcag atttaaaaaa tgcataatcg atcttatatt
     1681 cgatgtccaa taaaattgag tatactctcc cacaggaagg actattctgg ccatttctct
     1741 tgcaataccc aattttaaaa gcttctgata tgtttcatat gcattcttat aactttcttc
     1801 aataagcaac aaagcctgtt cttttttgtt atcatcctca catatatatg aactttgacg
     1861 gttttcctcg tcttgtactc ttatgtggtc aggcaggtaa aactcctcac tgtatttggt
     1921 agtatacctt ctgctgatct cattgtaaga atttcctatt cggtgtctca gccactgcct
     1981 ggcaacgaaa ataggacatt tcacatgaaa agtaagcgtt atgtgttcaa aaggtgattc
     2041 gtgcttattt ttcataagat actctatcag tgctctgtct ctttcaatat cagaagactg
     2101 ctctttggca tatgaaaccc ttgctgcttt aacggctgaa aaatcgtcac ccatcatatc
     2161 tataagagtc acaaaacctt tatccagaat actacatttt atctcctgca tgaatgccta
     2221 cctcacttat ttttttagca tttcttcata ctccttgttt tttttgcgtt tttccagtac
     2281 tatcattacc gttatacctc caattactat ccccagaagc ggaagaatgt aaaccatgac
     2341 tcctttaatc tttatatccc tctgcagatt atcaagttct gctctaatct caggctgatt
     2401 gggatataac gaaagtgctt tattgagtat gactcttgca agctgagcat tttcttttct
     2461 gtcttcctgc aaatatgaaa cccccagctt actccatata agtccgtttt tctcatcata
     2521 ctcaagagaa agcttatagt aattgatctt ttgaacatta gtctgggcaa tatctcctaa
     2581 aagttcgtat gtagaagcta aaatattctt tcttcccgaa cttttagtta tacttgatgc
     2641 gagctccctg gctttatctt tgttttctaa attaaggtat gattttgctg caatgagatg
     2701 tatctcatcg gcactttcgc ctaattttat gtagttttcg tatagatctg ttgccttttt
     2761 ataatcttca ttctgatagc tcataacaat gtactcatat aaaattttta tattatcaga
     2821 atactcagta tatatatctt ttattatctg aattgcctga gaactatctc caattacttt
     2881 tgacttccac agaaccttgg ctttataaaa agaagaaagc ggattggttc ctgcaagctg
     2941 ttcaagatta ttgcatgaat ctattatttc ctgaccgacc tgaatttctc cagatctctc
     3001 ccagtctgaa tataaaaact caatgaaaaa agcattgatg attatggact gaggataagt
     3061 ttcttttgct gatctgaact ttttgcctgg ggataagaac tcctgcggat tgatgcttga
     3121 aaaaatatct aaaacagctg tatcgtcagg gaaaaaaaga tccatgtttt ctgaaaatat
     3181 cgtgttggca gtacttttat actcttcata accacttttc cagtatttaa gataaaaacc
     3241 aagcacactg agctcttttg aatctgaaac aagcttttca tcatcaaaga caaaaacata
     3301 tctgtccaga gaagaagcga agactgctat aactgtcaga aataccaaaa aaaacgctac
     3361 tgtaagcttt ttcattagtt gcctccgtta atcttttgaa tatcctttta aaactatttt
     3421 atcttcaata ctcatgaaca tgacctttac catcgcacgg tcaggaaaaa atctgcgttt
     3481 atgtatggtt atcgatgctc ccggatagac acattctttt gcaagaataa aaccgtcatt
     3541 ctcagaagat tcaattattt ttttaatttc ttccagtctt ttttcattgc tctcaagatt
     3601 gtctttaaga gtttttgctg tctgaagact tctcttgtac tgttctatct tttcctgatc
     3661 aagtctgcct tcattctgaa gctttaccat acctttaagt ataaggctaa gtttccttaa
     3721 attttcaaga tcaattgcta tctgggagct caatatcgta agttcctcgt ttagctctgg
     3781 ctgaactccg acctcaagat ttgtggtaac ggtcatttca ctcccaagaa aatctgcctc
     3841 tatcttattt ccggctatta tatttcctcc tattattctt ccgttctttc ctattacaac
     3901 aacagtttcg cctgctttta ccttagaatt gattatagcc tgttttacat aaatattctt
     3961 ttttgcctcc acctcagcat tttctatgaa tgcagcttta acggtgtcag ctcttataat
     4021 cccttttcca gctcctttta ttccggaaac agtaatctca ccgccagcaa ttactgtggc
     4081 tgactcaact atgcctttta tctctatgct ctgttctgct tccactataa atccaggcct
     4141 cacattcccg tttataacaa gagaaccggg aaatcttaca ttaccaatat tataatctat
     4201 atccccttct atacgtttta cttccgacac atcaatattc aaagcgtcat ttgcttcacg
     4261 tttaaaaaca agttcacctg aaacctttgc gattacatat ccatcctgga taaaagcatt
     4321 tttccccaca gtacacttag cttcttttcc aataagccct tctatctcct taccataaac
     4381 atccttaccg ttttctcctt ttgcaggcgg gagtatctga gctaacttct cattggcttc
     4441 aacaattaaa aaatttaaga cttctctcaa gtcaaccttt ttttcggtat cgtaattcac
     4501 tttttccctt ctgaccaaaa tattaacttt tccatcgtca ccaactttgg gaccttttcc
     4561 gtaagctaca ggaatttctg agttaaactt tacattagca caaagctctc gtattgtttc
     4621 catgcttatt ccgtaagaaa tcccgttact ctgaagaaaa gcaaggacat catcaacttt
     4681 taagtatact ccatcatcaa gtagactgac cgaagcaacc atgctgtccc cggatacttt
     4741 cagtttgagt ttggccattt ggataacccc cggattataa ttttttgtat aagtataaaa
     4801 ggttcccctc tggaacatca tcccatctca tattcccgaa aaccttttga attataaaaa
     4861 ctcctagacc tccgtcccta tagtcttcaa gatctctggg ctgtattttc tgcggatcaa
     4921 cctttctgcc gaaatccctt atttttatac aaaagactct ttcctcatca atagaaaact
     4981 caacattaat actatgattt tcctcaaagt tatatgtatg ttctataaca ttggctattg
     5041 cctcatttag tgcaagttcg aacataaata tttcatcttc ttcattataa ttattaactc
     5101 ttagaaattc cttcagtacc tgccgtaagg ctttaatata actactttta ctctctatct
     5161 ccacccttat ctgactcata agcccccccg taagtcaaac gactatgaat ttggcacgcc
     5221 aatttcctat attaatacga ccgcttttgg ttattcctat ctctttagct tcagaagaat
     5281 taataactat gctttttggc cttaaactct ttaaaatctt cataggatct tcttttatat
     5341 cctcaagaca tatatcataa ggaattctta tttcagctga tccgttgaat ctgaaaagat
     5401 tccctgcttt caactctcct acaatctccg aaagttcttt gaatctattt ttttgagata
     5461 aaatatgaag ctcttcagat tctttttttg aaaaagaaca accgcaaaaa gtctgtctat
     5521 atataccaaa tcttctgcaa agatctgcac caaaagaaat accattatca gagcggaaat
     5581 tataaaagtc aaaggctact ctatacctta ccgccatagc ttctccaata cgccttataa
     5641 actcctgctc tttcctcgga cttgcagtaa gtgtcgtagt aacatgcaaa aaaccatttt
     5701 cagcggcagt ctcaaatgtt ttttctagtc tcatataaat acatttttgg catctgtcca
     5761 taccagctat attttctgaa acaaatgtaa gataacggtt tggatcgtac agggattgaa
     5821 tttcttcaaa tccatactcc aaaggtatct ttttaagctg acttttcctc agaaggtact
     5881 cattgctgtc atcgatattg ggattataga aatacacata cacctttaga tcttcatttg
     5941 ccaaaatttt acttgctgca agaagatccg gcacacaaca gacatgtacc agcatctgct
     6001 tcaaaattct atataccccc ttattttttc tatttgttta tcgctcatac ctgtaatttt
     6061 tttcaattcg tccatatttt caatagtttt tatctttctg tactctaaaa taatatttgc
     6121 ggtcttttcg ccaattcctt caagttcttc aagtttttgt atagaatcag aattgatctt
     6181 tactttagtc tgccggtatt caaacttttt tgttgatatg gttacataat tccttatttt
     6241 ttcaaaagtt ttatcgccta ttccagttac atttttaagg tcatctatgc atgaaaaccc
     6301 gtgttgttca cggtattcaa ttatattcaa agctttggag tatcctatga atgggatatt
     6361 cataagttct ttgatgtcag ctgatgagat atccacaaga taaactcctt gagtttcttt
     6421 ctcattatct gtttgattta ttatcgctat tacacctatt agagttactg caaatactgc
     6481 taaaaatgtg aaaaaaagct ttttcatatt acttatgtcc ttggttcaaa atccatcaat
     6541 cttatatatc tgccttttac atcaaccccg atcagtttgt agatactgtc aagatcaaga
     6601 gatgcattaa ttgtatcggc gctttttcga gaggtaaaaa tgccgagcat cagagcatgg
     6661 tattctttgc ctgactgagc atagtatcct ttatatacat atgcggcgac tccttctttt
     6721 cttaaaagat tacacgcaac ataagctcct tcaatattgg aatacgttgc aagttgtatg
     6781 gagtaaagac tatttaaatc attgttggta aattctttgc tataaagaac atactcatca
     6841 ccattttttg aaatcataaa aggtatttct tttttatatg cctgaactgc ctgtagatat
     6901 gcttcttttt catcaatggt tctggcatgt ccgccagact gactttctat actcatactg
     6961 gcaaccgcca aaagattgta ataatttaca tcttttagct cgtagtttat cttaggagca
     7021 ttgggctcgg tcattgaata agaaggcact ccctgcatat tctctgtcgg tgtagactgt
     7081 attgaatcca gcacaacttc acttctaaga tcattgatcg aaagtttcaa atcctttatc
     7141 ttgtttttta aagatatggt ataaattgtg aggataaaag ttagaacggg tactataaat
     7201 acgattataa caaaaaagat aaaagatttg ttatactttt tcttcaactc agtcacccct
     7261 gttagattgc ataaactcca aaagaacttc ttttaactct tctttatgca gaagcacttt
     7321 cctgaaagaa ctctgggaat gtggaactac ttcttctata accgaaccga tcttatcaaa
     7381 tgcaatatac tgcataatat ttttattata aagttcaagt tcaatcatat ctttcttgat
     7441 tgaaacatac tgctcatagc ttatataact atttgaaaga cttatataag ctaaaaaaag
     7501 aaagatattt gaaaatacaa cagtgtactc tttcttaaaa tccccaggct tactataaaa
     7561 atactttatt aaattcgatc cgggcagaaa aatctcctga gaactataaa gataggttaa
     7621 aaatataaga ttttcatata tgtattcttc tacgtcaaga cctgaaaaat tatgcgcata
     7681 tttcatggaa agcttacagt aatttttatc aaaaagaaag ccaagaaaat agcctgaaaa
     7741 aaacattttt cttaaaatat gtccggaaat ttttgaaaga aattcaggat caatatatat
     7801 aatatccgga tatcctctaa ctttaagata attatatgaa taattcatat caagaaaaaa
     7861 ataaccgcta atgttagcct gaataaagga gttcaatgta gtaggataaa gtatcagatt
     7921 tttaacatta caatttattg tatttaccgt atacccacat aaatcaagaa ctgctccgcc
     7981 acctatccca ataagtgttg aaatatcatt gtttacaatt ttatctgcaa cttcaagata
     8041 tttatcaata aacttagctt cttcgcctcc acgaacatac tttataaaag acttgctttc
     8101 catcatggtt tttattttgg aatcagctac tgtataggtc ccatacttct gtgaaagtgt
     8161 ctttattgtt tttttgtact gtccgtatcc tatagttatt tctatttttt ttccttcatt
     8221 gccgataaat gaaatgcttc tcaaaaaaat cagcctttca gactatatca atagtcctgc
     8281 cagaaaagaa tcaacggcag atgcgtttgc aataggatta tagccgcctt cctgaataac
     8341 aagtttaaat atatcaagtt ctgaaagtat ctttccaatt tcaaaaaagt cggaatctcc
     8401 caacataaaa ttaccaaggg gatcatcttt atgggtatct gttcctagcg atacgataag
     8461 atacgaagga tcataatcct gtatctcctg cgctgctttt tcaagtgttc taaga
//
LOCUS       DAOSHR010000084         8370 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_7276_length_8370_cov_6.634997, whole genome shotgun sequence.
ACCESSION   DAOSHR010000084 DAOSHR010000000
VERSION     DAOSHR010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 8370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8370
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7276_length_8370_cov_6.634997"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="PLU28_05905"
     CDS             complement(<1..275)
                     /locus_tag="PLU28_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34632.1"
                     /translation="MQFFQFMQVYIVSIGVLAVILFKIICERDYDHFQNKLFATIVAI
                     TINVLIVEALGWRLDGMAGSSARLFLILDTVILMIITLYPGILWIMYL"
     gene            complement(389..1747)
                     /gene="miaB"
                     /locus_tag="PLU28_05910"
     CDS             complement(389..1747)
                     /gene="miaB"
                     /locus_tag="PLU28_05910"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOG34633.1"
                     /translation="MNFLIKTFGCQMNVNDSEIMSGLLTQAGYLWTENPEDADIVLIN
                     SCAVREKAENKMYGTIGQFLKIKKSRKLIIGVGGCSAEKEGKAILERFDGVDFVFGTR
                     NISDIVTLIQRAQKHKRFVDLSDKLDCVSHELPKNPTSRHHAWITIIYGCNKYCTYCI
                     VPYTRGLEKSRPYEDIINEVYDYAAKGYREITFLGQNVDSYGKDFSDGKPKLDLLIKK
                     ASEIEEIKRVWFLTSYPTDITDSLIDEIAASRNAARNVHLPVQAGSDSILKSMNRRYT
                     RQQFIDLVKKVKDKIPSVTISSDIIVGFPGETEEDFQQTVDLIKECRFERVNLAEYSP
                     RQGTIAYKYKPDNIAHEEKNRRLMYLMELQKEINREENEKYRDKLLRIIPEAKASDTA
                     LYGRTMNNKIVIFNSQDASLIGKFADIKIKRITAGPLYGEMTKNENDAGFDIQNDPTF
                     MI"
     gene            complement(1799..2623)
                     /gene="rpsB"
                     /locus_tag="PLU28_05915"
     CDS             complement(1799..2623)
                     /gene="rpsB"
                     /locus_tag="PLU28_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOG34634.1"
                     /translation="MSVVSMKQLLEAGAHFGHRTRRWDPKMKPYIFTERKGIHIIDLQ
                     KTLQSVNDAYDFIRKVSSNGGSILFVGTKKQAQQIVADEAKRCGGFYVNNRWLGGLLT
                     NFTTIRKRINRLEELLAYVESEEFTKLPKKEQSHFKRILEKLEKNLGGLRGMTKLPEV
                     MYVIDPRKEEIAIKEANDLGIPVVSIVDTNCDPDVIDWIIPANDDAIRAVMLITAKMS
                     DAFIEGREGRTDAVQAPASAGTAEDIPEEIANKLDAEEKYSEIASNLVEEEEEEKK"
     gene            complement(2755..3102)
                     /gene="rsfS"
                     /locus_tag="PLU28_05920"
     CDS             complement(2755..3102)
                     /gene="rsfS"
                     /locus_tag="PLU28_05920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOG34635.1"
                     /translation="MELDKVKTVLKQVISLLDDKDAKDIKIIDMKESSLIVDYFVICT
                     VDSDTLMSALRDYVLKIFKDNKIDLITNDKGKNYDWMIIDAGSIVVHFFTKEGREFYD
                     LEGLWSEFDRIEV"
     gene            complement(3124..4950)
                     /gene="glmS"
                     /locus_tag="PLU28_05925"
     CDS             complement(3124..4950)
                     /gene="glmS"
                     /locus_tag="PLU28_05925"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HOG34636.1"
                     /translation="MCGIFGFVGEGLNVQDLINGLKKLEYRGYDSAGIAIENEKNISI
                     YKKEGRIYNLESEIPQLMEKKISTALGHTRWATHGGVSDQNSHPHTDCTGKVAIIHNG
                     IIENFQYLREKLIKNGHIFKSETDSEVIAHMVEENYSGNLLECVIKVLHELNGAYAIG
                     VIHTDEPNCIIAARKGSPLVISAGSNISMLASDVTPILRYSKDVNFLNDGEIAKLTNN
                     GYELYDLQGNKIERKPIHINWDEACAERSGYNHFMEKEIFEQPIAIKSATIGRLKDGI
                     PCIKELEPLKDFIETKLEKIYIVACGTAYHAGLAMKYFMQYYSNIDVSIEVASEFRYM
                     NPKIDGKTLTIAISQSGETLDTLEGVRIARKNGSHVLTVCNVVGSTIPRESDSVIYMN
                     TGPEIGVAATKTYTAQIAILYIVGAQIALWKNLYSEDIKNALCQIENLPEYFNTVLGY
                     SQKIKELSKSYCGYRNMMYIGRRFGYTAALEGALKLKEISYINATGYQAGELKHGPIA
                     LLDQEFPVFAMVCDDDIKEKMISNIMEVKARKARVVAVGIEGDEKIKTLTDDYILIPK
                     CEQAVFPLAVAPVMQLFAYNVALLRELDPDKPRNLAKSVTVE"
     gene            complement(4965..5954)
                     /gene="plsX"
                     /locus_tag="PLU28_05930"
     CDS             complement(4965..5954)
                     /gene="plsX"
                     /locus_tag="PLU28_05930"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HOG34637.1"
                     /translation="MSKIRIGLDLYGGDNAPVSLIQGAIFALQAQDMPDLEICFMGGK
                     SSDFSELTEKGLTNFSFTPASNNVTNDTKPTEVLKLKDSSIYIGCEKLKTNELGAFVS
                     CGNTGALLSAGIFVAGRLPGIKRPSLVAALPGKNSKVRVLCDIGANADVKPEFLLDFA
                     KQGNAFAKFLGIENPRVGLLNNGTEEEKGSSVTKEAHQLLKGLQGINYIGYIEAREMF
                     GNTFDVLVTDGFTGNAVLKTMEGTAYYILDEMKQLIKTGSIKDKLGGLLIKKRLKLLK
                     SKLDYKQYGGTFLLGVNGVLVKAHGSSDAQAVYNAIKVAYRAVKDELISKISL"
     gene            complement(5957..6139)
                     /gene="rpmF"
                     /locus_tag="PLU28_05935"
     CDS             complement(5957..6139)
                     /gene="rpmF"
                     /locus_tag="PLU28_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOG34638.1"
                     /translation="MAVPKQKPSKARTRRRRAKFYAAIKVDVVKCPKCGEPKMPHRVC
                     LNCGYYGNKQILEIGE"
     gene            complement(6168..6719)
                     /locus_tag="PLU28_05940"
     CDS             complement(6168..6719)
                     /locus_tag="PLU28_05940"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HOG34639.1"
                     /translation="MLNEDLYSKDLIINLESFDELLEKNFEINNWHSIQLLSEEGEFL
                     DTISVSLEVRKSGDGIIAKGRVKTLLGLHCSRCLKKMEQSVDETFDSVYVSSLFEEKG
                     LKTEHLESLDDTFFYDGSILDLTDRVIEAIILSVQEKPLCSQECKGLCPVCGTDLNEN
                     PQHICCEKEIDPRFEVLNKLLEK"
     gene            6914..7111
                     /locus_tag="PLU28_05945"
     CDS             6914..7111
                     /locus_tag="PLU28_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HOG34640.1"
                     /translation="MKGTVKWFDAKKGYGFVTGDDGKDVFIHFSAIKMDGYKKLEENQ
                     KVEFDVVDGQKGPQAANVKII"
     gene            complement(7163..7921)
                     /gene="deoC"
                     /locus_tag="PLU28_05950"
     CDS             complement(7163..7921)
                     /gene="deoC"
                     /locus_tag="PLU28_05950"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HOG34641.1"
                     /translation="MNNSQLEKIISQELQTVQKEYSFIEKDIDIKPLEVAQYIDHTLL
                     KPTATVQDIKLLCRQAKENNFFSVCVNSAYVSLAKEELRSCKVKVATVIGFPLGVCDS
                     SVKVYETQSALDDGADEFDMVINVGMLKSLDYEYVYREIKDIVEQADKKTVKVIIETC
                     YLTREEKIAACVLSKLAGAHFVKTSTGFGTGGATMEDVSLMKFVVGEDVKVKASGGIK
                     DYNTAKKMIACGAQRLGASAGIKIIKGESSEEKY"
     gene            complement(7978..>8370)
                     /locus_tag="PLU28_05955"
     CDS             complement(7978..>8370)
                     /locus_tag="PLU28_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34642.1"
                     /translation="RLLAMNQEENGIFIPENERLDYDFIKFFNMKFEQNANSAFQKIV
                     DTFGNLRIFLTADSIAQKGLKTGTFYLPKDSYSILLKGPDYCTELRNTLAGLKQNYNK
                     EFDFIIDIPFYEVLRIIFGWRTCQKING"
BASE COUNT         2207 a         1772 c         1471 g         2920 t
ORIGIN      
        1 agatacatta tccacagtat tccgggataa agggtaatta tcatcagtat gacagtatcc
       61 agaattaaaa ataatcttgc actactgcca gccatgccat ccagccgcca gcctaaagcc
      121 tcaacaataa gtacatttat ggtaattgca acaattgttg caaaaagttt gttttgaaaa
      181 tggtcgtaat ctctttcaca aataatttta aaaaggatta ctgcaaggac gcctatcgaa
      241 acaatatata cctgcatgaa ctgaaaaaat tgcatacttc ctcccaattt cacaaacttt
      301 ctgtgtcgaa tcagataaat tatatcatac ctgttatact ttatcggaag aaaaatgcct
      361 gcagtttaat tttgcaggca tttttaattt atatcataaa tgtaggatca ttctgaatat
      421 caaatcccgc atcattttca ttttttgtca tttcaccgta aagtggccca gcggttattc
      481 tttttatttt tatatcggca aatttgccta taagagaagc atcctgtgaa ttgaagatga
      541 ctatcttatt gttcatcgtc ctgccgtaaa gagccgtatc gctggctttg gcttctggta
      601 ttatcctcag gagcttatct ctgtacttct cattttcttc acggtttatt tctttctgaa
      661 gctccataag gtacataagt cttctgtttt tttcttcatg agcaatgttg tctggcttat
      721 atttatatgc gatagttcct tgccgcggag agtattctgc aaggttaaca cgttcaaatc
      781 ggcattcttt tattagatcg actgtctgct gaaagtcttc ttctgtctct cccggaaatc
      841 ctacaataat atcagaactg atcgtcacag aaggtatttt atcttttact tttttcacaa
      901 gatctataaa ctgctgtctt gtatatcgtc ggttcatact tttgagtatg ctgtcgcttc
      961 ctgcctgtac gggaaggtga acatttcttg ctgcattgcg tgatgcagct atttcgtcaa
     1021 ttagcgaatc ggtaatatcg gtaggataag atgtaagaaa ccatactctt ttaatttctt
     1081 cgatttccga agcttttttt attaataaat caagtttcgg ttttccgtca gaaaaatctt
     1141 tgccgtaaga atcaacattc tgtcctaaaa acgttatttc cctgtatcct tttgctgcat
     1201 agtcatatac ttcatttatt atatcctcat aaggacgact tttttccagg cctcttgtat
     1261 atggaacgat acagtaggtg cagtacttat tacagccgta tattatagtt atccatgcgt
     1321 gatgtcggct ggttggattt tttggaagtt catggctcac acagtcgagc ttgtctgaaa
     1381 gatctacaaa gcgtttgtgt ttctgtgccc gttggattaa ggttacaata tcgctgatat
     1441 ttctggttcc aaaaacaaag tcgactccgt cgaatctttc cagaatcgct ttaccttctt
     1501 tttctgcaga acagccacct acacctatta tgagctttct gctttttttt atcttgagaa
     1561 actgtccgat ggttccgtac attttgttct cagccttttc tcttaccgca catgagttta
     1621 tgagaactat atcggcatct tcaggatttt ctgtccatag ataccctgct tgggtgagaa
     1681 ggcctgacat gatttcggag tcgttgacgt tcatctgaca tccgaaggtc ttgattaaaa
     1741 aattcattct tcctccttat gaaaaagacg ccgtaacggc gtctttattt ggtttatttt
     1801 actttttttc ttcctcttct tcttcaacaa gattgctggc aatttcactg tatttttctt
     1861 ctgcatcaag cttgttggct atctcttcag gaatatcttc tgctgtgcct gctgatgcag
     1921 gtgcctgtac agcatcggtt cttccctcac gtccttctat aaaagcatcg gacatctttg
     1981 ctgttatgag catgacagcc cttatagcgt cgtcattcgc aggaattatc cagtcaataa
     2041 cgtctggatc acagttggta tcaactatcg aaacgacagg aataccgaga tcattggctt
     2101 cttttatggc aatttcttct tttctggggt caatgacgta cataacttca ggaagcttgg
     2161 tcatacctct taaaccgcca aggttctttt caagtttttc aagaattctt ttgaagtgtg
     2221 actgttcctt ttttggaagc tttgtaaact cttcggattc aacataagcg agaagttctt
     2281 caagtctgtt gattcttttt cttatagttg tgaagtttgt aagcaaacct ccgagccatc
     2341 tgttgttaac atagaatccg ccgcatcttt tggcttcatc ggccacgatc tgctgagcct
     2401 gttttttggt tccaacaaaa agtatgcttc cgccgtttga agaaactttt cttatgaaat
     2461 cataagcatc gtttactgac tgaagagtct tctgaaggtc gatgatatga attccttttc
     2521 tttctgtaaa tatgtacggc ttcattttcg gatcccatct tctggttctg tgaccaaagt
     2581 gagctccggc ttccaataac tgtttcatgc ttactactga catcctgcac ctccgctgaa
     2641 ttttgatttg gttttcatcc tccgcttttc tttttaccgc accaacccta aagagtatcc
     2701 gggcaccgtg gtggggaaca aaaaacgtgg gctttatccc tttaagggat tttattacac
     2761 ttctattcta tcaaactctg accatagtcc ttctaagtcg taaaattctc gtccctcttt
     2821 tgtaaagaaa tgaactacta tagatccggc atcgatgatc atccagtcgt agttttttcc
     2881 tttatcgttg gttataagat caattttgtt atctttaaag attttcagga cgtagtctct
     2941 cagcgcactc atgagggtat cggagtcgac tgtgcatatc acaaagtaat ccactatgag
     3001 cgaagactcc ttcatatcga ttatttttat atctttggcg tctttatcgt ccagtaacga
     3061 aattacctgt tttagaactg tttttacctt atctagttcc aaaaaaaact cacctccgta
     3121 attttattca acagttacac tttttgcaag atttctgggt ttatcgggat caagctccct
     3181 cagcaatgcc acgttatagg caaaaagctg cataacagga gcaaccgcaa gcggaaaaac
     3241 cgcttgttcg catttgggaa taagtatata atcatcggtc agggttttta ttttttcatc
     3301 cccttcgatt cctacagcga caacccttgc ttttctggct ttgacttcca ttatgtttga
     3361 tatcatcttt tcttttatgt catcatcaca taccattgca aaaacaggga actcttgatc
     3421 taaaagtgct ataggtccat gtttaagttc tcctgcctgg tatcctgtgg cgtttatgta
     3481 actaatttct ttaagcttaa gtgccccttc aagagccgct gtatatccga atcttctgcc
     3541 aatatacatc atatttctgt atccgcagta agactttgaa agttctttta tcttttggga
     3601 gtaacccagt actgtattga agtattcagg aagattctct atttggcata atgcgttttt
     3661 aatatcctct gagtacaggt tcttccatag tgcaatctga gctccgacaa tatagagtat
     3721 agctatctgt gccgtatatg ttttggttgc cgcaacgcct atctctggcc ctgtgttcat
     3781 atatattaca gagtcagact ctcttggaat ggttgaaccg actacattac atacggtaag
     3841 cacatgagag ccgttcttcc tggcaatcct tactccttca agagtatcaa gagtttctcc
     3901 ggactgcgaa atggcaattg taagggtttt cccgtcaatc tttggattca tgtatctgaa
     3961 ttcggaagct acttctatac ttacatcaat atttgaatag tactgcatga agtacttcat
     4021 tgcaagacca gcatgataag ctgttccgca tgccacaata taaatctttt caagcttggt
     4081 ttctataaaa tcttttaacg gttcaagctc tttaatacaa ggaattccgt cttttaaacg
     4141 tcctatggtt gctgatttta tggcaatggg ctgttcgaag atttcctttt ccataaagtg
     4201 attgtagccg cttctttcgg cacatgcctc atcccagttg atatgtatag gttttctttc
     4261 aatcttattt ccttggaggt catacagttc ataaccgtta ttggtaagct tggcaatctc
     4321 tccgtcgttt aaaaagttga catcttttga gtaacgcaga ataggagtaa catctgaagc
     4381 aagcattgaa atattgcttc cggcggatat tacaagcgga ctcccttttc ttgctgcaat
     4441 tatacagttg ggttcatccg tgtgtattac cccgattgca taggctccat tcagctcatg
     4501 aaggactttt atgacacatt caagaagatt acctgaatag ttttcttcaa ccatatgggc
     4561 aataacttct gaatcggtct cggatttgaa aatatgcccg tttttaataa gcttttctcg
     4621 caggtactga aagttttcaa ttatcccgtt atgaattatg gctacttttc cggtgcagtc
     4681 ggtatgaggg tgcgaatttt ggtcgcttac cccgccgtga gtggcccatc gggtatgtcc
     4741 cagcgccgtg gaaatctttt tttccatgag ctgtggaatt tcagactcaa gattgtaaat
     4801 tcttccctct ttcttgtaaa tgctgatgtt tttttcgttt tcaattgcta taccagctga
     4861 atcataacct ctgtactcaa gttttttcaa cccattaatc aagtcttgga cattaagtcc
     4921 ttcaccaaca aacccaaaaa ttccgcacaa aatattaccc cctgttacag cgagatttta
     4981 gaaatcagtt cgtcctttac tgccctgtat gcaactttta tggcgttata aacagcctgt
     5041 gcatctgaag agccgtgcgc ttttacaaga acaccgttaa ctcccagtaa aaaagttcct
     5101 ccatactgtt tgtagtccag tttggatttg aggagtttaa gtcttttttt aatcaaaagc
     5161 cctcccagtt tatcctttat actaccagtt tttataagct gtttcatttc gtcaaggatg
     5221 taataagctg ttccttccat ggtttttagt accgcattac cggtaaatcc atcggtaaca
     5281 agtacatcaa aggtattgcc gaacatctct cttgcttcta tgtaaccaat gtagtttatg
     5341 ccctgaagtc ctttaaggag ctgatgggct tctttggtta cggaagatcc tttttcttct
     5401 tctgtaccgt tgttaagaag tcctactctt ggattttcta tccctagaaa tttggcaaag
     5461 gcgtttccct gcttagcaaa atccaaaaga aactctggtt tgacgtcagc attggctccg
     5521 atatcgcaca gaacccgtac cttgctgttc tttcccggca aggcagcaac aagcgatggt
     5581 ctttttatcc cgggaagcct tccggcaaca aaaatccctg cggacaaaag agcgccggta
     5641 tttccgcagc tcacaaatgc ccccagttca ttggttttta atttttcaca tcctatgtat
     5701 atagacgaat ccttaagttt gagaacttct gtcggtttgg tgtcgtttgt gacattattg
     5761 ctggccggag taaagctgaa gtttgtcaga cctttttctg taagttctga aaaatcggag
     5821 ctttttccgc ccatgaaaca tatttccaga tcaggcatat cttgggcttg taaggcaaaa
     5881 atcgcgccct gaatcaatga aacgggcgcg ttatctccac catataagtc caatcctata
     5941 cggattttag acataattat tctccgattt caagaatctg cttgttccca taataaccac
     6001 aattaagaca tactctgtgt ggcatttttg gttctccgca tttcgggcat ttaactacat
     6061 ctacttttat cgctgcataa aactttgctc ttcttcttct tgttcttgct ttagatggtt
     6121 tttgctttgg aacggccatg gaaaatacct cctctataag tataaattta tttttccaga
     6181 agtttattaa gaacttcaaa acgcggatca atttcttttt cacagcagat atgctgtgga
     6241 ttttcattta gatccgtacc gcatacagga cacaatcctt tacactcctg agaacataaa
     6301 ggtttttcct gaacagataa tattatagct tcaattactc tgtctgtcaa atccaaaatt
     6361 gatccgtcat aaaaaaaagt atcgtcaaga gactccaaat gttcggtttt taatcctttt
     6421 tcttcaaaaa gactgcttac ataaacggaa tcgaacgttt catcgacgct ttgttccatc
     6481 ttttttagac agcgtgaaca atgcagacca agcagcgttt ttaccctgcc cttggcaata
     6541 ataccgtctc cggatttcct aacttcaagg cttacgctta tggtatcaag aaactctcct
     6601 tcttccgaca gaagctgaat gctgtgccaa ttatttattt caaagttttt ttccagcagt
     6661 tcgtcaaaac tttcaagatt tattatcaga tccttggaat atagatcttc gttaagcatc
     6721 aacatcacct caagtagtat attttacttc ttattctttt aattggtggg aatagcatgt
     6781 aaatattttg aaaaactttc gatgttacac aaaatttact tgatttagtt taaattattg
     6841 tttataatct atctatatta taccgatcgt tgcattgggt aactcggtaa cgtttttacg
     6901 gaggtaaggt tttatgaaag gaacagttaa gtggtttgat gcaaaaaaag gttatggttt
     6961 tgttactggt gatgatggaa aggacgtatt tatccatttc tcagcaatta agatggatgg
     7021 ctacaagaag ttagaagaga accagaaagt tgaatttgat gttgttgatg gtcagaaagg
     7081 tcctcaggct gctaacgtaa aaattatcta aaacctaaaa tcaatagccc ctacgaaagt
     7141 aggggctttt catttataaa gatcagtatt tttcttcgct actttcccct ttgattatct
     7201 ttattccggc gcttgcacca agtctttgtg caccacatgc aatcatcttt tttgctgtat
     7261 tgtagtcttt tattcctccg gatgctttaa cttttacatc ttcccctaca acaaacttca
     7321 tcagggatac atcctccatt gttgctcctc cagtaccaaa acccgttgaa gtttttacaa
     7381 aatgagcgcc tgccagttta gaaagtacac aagcagcgat tttttcctcg cgagtaagat
     7441 agcatgtttc tataataacc ttgacggttt ttttatcagc ctgctcaaca atgtccttta
     7501 tctccctgta tacatattca taatcaaggc ttttaagcat tccgacattt ataaccatgt
     7561 caaattcgtc tgctccgtca tcaagggcgc tctgtgtttc ataaaccttt acgctgctgt
     7621 cacatactcc caaaggaaaa ccgataacag ttgctacttt tactttacat gaacggagtt
     7681 cctcctttgc aagagaaaca tatgccgaat taacgcatac cgaaaaaaag ttattttctt
     7741 tggcctgacg gcacaaaagc ttaatatcct gaactgtcgc tgtaggcttg agaagtgtgt
     7801 gatctatata ctgagctact tcaaggggtt taatgtctat atccttttct ataaaactgt
     7861 actctttttg aacagtttgc agctcctgac ttattatttt ctctaattga ctattgttca
     7921 tactctccct cctggattca tatgttgata taacattata attttattgt atctgcctta
     7981 cccgttgatt ttctgacagg tacgccatcc gaagataatt ctcaacacct cataaaacgg
     8041 tatatctatt ataaaatcaa actccttgtt atagttctgt ttaagaccgg caagcgtatt
     8101 tctcaattca gtgcaatagt ctggaccttt gagaagtatg ctgtaactat cctttggcaa
     8161 ataaaaagtt ccggttttaa gacctttttg tgcgatgctg tctgcggtaa ggaatatccg
     8221 cagatttcca aatgtatcta ctatcttttg aaatgcagaa tttgcatttt gctcaaactt
     8281 catgttgaaa aactttatga aatcataatc aagcctctca ttttcaggta taaatatgcc
     8341 gttctcctct tggttcatag ctagaagtct
//
LOCUS       DAOSHR010000085         8349 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_7302_length_8349_cov_6.046178, whole genome shotgun sequence.
ACCESSION   DAOSHR010000085 DAOSHR010000000
VERSION     DAOSHR010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 8349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8349
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7302_length_8349_cov_6.046178"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(112..1935)
                     /locus_tag="PLU28_05960"
     CDS             complement(112..1935)
                     /locus_tag="PLU28_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34643.1"
                     /translation="MRKAVVIFLSFITIIAGFAFSATIKTTDGNSIKVTVVSVKDGKV
                     SIKGFGTAISLPLDSINMISFDDQTFDINGVVIDKMNFSGYVSEYSLETNDATVTTTS
                     GRFYLKDLNVLKFWNVRQNTLKDEITNDYSGKKFKAFLGENTLLTGDITTINNGKILM
                     KVDDMGDAEFDIKYVVSLIRDQKTEEVLPNSVMLAKGYTLNFQSIDLFNDEINLKLAF
                     GFFKTDIKNVTLITGTGTEPPKLPYYFVMNNNVRFYGNLEDVKDNKIVVKSNFGINEI
                     SQEKIFSFVKIPPENRFILSTDTQMMYGEYLKKDGALNLKSKVKNEDIKTVKLYVSEE
                     SEMLKNSISQIAEIPVNTETKVLQISDLLYLFESETIKRYDKETFLEKNALKTKMLYP
                     NYIINTADNQYILNTLGSIYSSKFDLEKTLDNSQITTSFLSGFDLYLGTNIGDIYKID
                     VNAKEFAVLKIASFQEEIAMIAESSNGIYVLTKNSGKLYSLKDKQNLFNFDFRINKAI
                     LDGEILYAIDFNKNLNVLNIGQKINKPFKYLKNINDLSSSKEYVFIALEDKISVFNKK
                     TQLIEKEYYTNYIPLKIEVNTNKLYILSDKSVKIFEAFESY"
     gene            complement(1952..2920)
                     /locus_tag="PLU28_05965"
     CDS             complement(1952..2920)
                     /locus_tag="PLU28_05965"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HOG34644.1"
                     /translation="MKEEILKEITQASAIIVAGHIDPDGDDVSSVSSMVMGLEKLGKR
                     AIGVIDSQIPDYLKNFQFVNDKIREMSCVEKDPADLLIIVDASSPDRVGKVQQLFSYR
                     RVVVIDHHVTNLSFGHVNWVDASYASCAQMVLEVLTSLGVEYDKELATANLLGIATDT
                     GFFKYSNVNEKVFNDCAFLLSKKAELSYITNMILENVPLESKLLLKDVLNEMKLVNDN
                     TLAYVAIPREMSTRYGFDPVNVPPFVSELRSIKGVEVAVTFIEYEKNDWHVSLRSKSY
                     VDVSQVALKYGGGGHVRAAGFSLQTGDIKSEIRAIIENIISFMKTR"
     gene            complement(2936..3748)
                     /locus_tag="PLU28_05970"
     CDS             complement(2936..3748)
                     /locus_tag="PLU28_05970"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOG34645.1"
                     /translation="MLTMVRRACEYINSQIEEKPIIGMILGSGLGYLADKIQDQRVIN
                     YKDIPNFPVSTVIGHEGKLVVGKLEGVQVITLKGRFHAYEGHKIRNIAFPIYVMKELG
                     VKGLILTNAAGGVNMMYKPGDIVANRDFINFSFKNPLIGPNKDEIGPRFPSMAFPCDS
                     NWIERVINQSKAAGIEIKSGTYCWFLGPSYETPAEIKLARKLGADLVGMSTMPEVIAA
                     NHCSIKVISFSAVTNMAAGILPQPLNHKEVLAVADQIKGKFESVVKTSIKLF"
     gene            complement(3770..4792)
                     /locus_tag="PLU28_05975"
     CDS             complement(3770..4792)
                     /locus_tag="PLU28_05975"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HOG34646.1"
                     /translation="MKIKFKDTLKIEPDKYILALDIPSAKLKKGIIISDSVRHSLRYQ
                     GLALVPTYENKMSSYNILDQSISKKTFSSIQKTWDKLRQNEKIDKELLSDSTNELVED
                     VMKKFGDINYIPLVKLNSFDQYTYAHTLNVGLITLLMAVEMGFGKKDLYELTYAGLLH
                     DIGKSRIDEKILNAPRKLTDDEYNIVKQHVVLGKLICQEQKIEGDILRGVYEHHERYD
                     GHGYIAGKKGTEISFYGRLISVADVYDALTSKRAYKDMWNYYKVVSYILQNAGKIFDP
                     EISSSFIKVFGVYPPGTMVKLNDSRTATVVAVRKGNELQPVVQVEDKIIDLSKEKFYI
                     MQVIGE"
     gene            complement(4776..5816)
                     /locus_tag="PLU28_05980"
     CDS             complement(4776..5816)
                     /locus_tag="PLU28_05980"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HOG34647.1"
                     /translation="MRKVKLSDLKAGFILAEHVTYNAVILLSEGNVLTEADIQKLKNR
                     GIREVIIYDEDDFILGKDIKVEFKDIPPIVEAKKYKEWNDQFEKIKDITKINEQPQEM
                     ESLVEDLHKTFIEKDEIILNLFHSIGDKALTLHSINTSIIASIISSHLKMPKVFSLEL
                     IKASLIHDLGYGFLNTRIIFDYDKSEDRFVKSHIIAGYKAFEPLKATLSKEIVNSILQ
                     HHERYDGKGILMGLRDERISPLVRIMQIGDAYDSYIEIGNNPYEAMSYLLKNAGKLFD
                     PYYVSIFFSVVGLYPSGTKVVLNDGRIAQVIKKGVAAVFPVVNVEGKSIQTGPETGFF
                     IKEVAEGNENKI"
     gene            complement(5841..7055)
                     /locus_tag="PLU28_05985"
     CDS             complement(5841..7055)
                     /locus_tag="PLU28_05985"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOG34648.1"
                     /translation="MYKIRTLNNNLRLISVPRNSMRSASIIFCVGAGSSDENETVSGV
                     AHFIEHVSFRGTSSRDMVSIKKPIEEVGGTLNAFTGNNLTIYYAKVPSTEVMTAVEIL
                     SDIVFKPLFNEADIEKEKDVIYEEISMYEDDPVDNVFENLFRNIYNPEFARPIIGNRK
                     NVENFDKSIITGFYNKFYKPSNVTVVLTGSYEEDKIADYLSLFEGKENFISDMKSPQL
                     KNKNIIVEKTKKDLSQHYIVNAFKAPSRSDEGYYPSLVLNTLLGSGMSSLLFNRIREE
                     KSLVYEVTSEYNAYKKSGVFLVFAATSGDKLTKYNNELENVLKSFYSDKDAEQWFNYG
                     KKRLIGKLTIDIESNVSLGMNILDLYLNNKEPITIDDVIKNIESVTLSQVLRAAGDIF
                     EGNKYVSVLKPE"
     gene            complement(7056..>8349)
                     /locus_tag="PLU28_05990"
     CDS             complement(7056..>8349)
                     /locus_tag="PLU28_05990"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOG34649.1"
                     /translation="KYLEKWTDNFYASKARLLNEFFEDEVKNTMRKMIIENNTRVDGR
                     NPDEIRPITCETHVIPRLHGSALFTRGETQSLGTVTLGISMDVQFVDTIFTEGEKRFM
                     LHYNFPPYSTGEVKSLRGISRREIGHGHLAERAHKNLIPDEKEFPYTIRVVSEILESN
                     GSSSMASVCSASLALMDAGVPIKKHVAGIAMGLIFEDEKFVVLTDILGMEDHLGDMDF
                     KVTGTRDGITAFQMDVKVAKVNEEVLREALEKARIGREKILSLMYDAMPQAREELSPY
                     VPAMLITAIPTEKIGEVIGPGGKVIKEINRRFEVETEIEDEGGIVKVSGQDKEKVKAA
                     IRYIESIVRIIKKGDIIEGEILRVEPYGLFVELSPGKSGLLHGSNLGEDPKKYKVGDF
                     IKIEVMSIEDNGKFQLKKEGFVKKTVPREKENNGGTKE"
BASE COUNT         2447 a         1697 c         1238 g         2967 t
ORIGIN      
        1 ggttaactat tccttttctt ttttataacc ttgtccacct ttcacagtgt ttattatctt
       61 ccagttggat tttaaataaa aaaagacaga ctttcgtctg tcttaaaaat atcagtaact
      121 ttcaaatgct tcaaaaattt tcactgattt atctgaaagt atatataact tgtttgtgtt
      181 tacttcaatc ttcagaggaa tatagttggt ataatactct ttttctatca actgtgtttt
      241 tttattgaaa acacttatct tatcctcaag cgctataaaa acatattctt tactggaaga
      301 caggtcgttt atatttttaa gatatttgaa aggtttgtta attttttgcc ctatattaag
      361 cacattaaga ttcttattaa agtctatagc atataggatt tctccgtcaa ggatagcttt
      421 atttatacgg aaatcaaaat tgaaaagatt ctgtttatcc tttagtgagt atagttttcc
      481 agaatttttt gtaagcacat atataccgtt gctgctttcg gctatcatag caatttcttc
      541 ctgaaaagag gcaatcttta aaacagcaaa ctctttcgcg ttcacatcaa tcttgtatat
      601 atctcctata ttcgttccca gataaagatc aaaaccagac aggaaggatg tagtaatctg
      661 tgaattgtcc agagtttttt ctaaatcaaa ctttgatgaa tatatgctcc caagggtatt
      721 aagtatgtac tgattatcag ctgtgttgat tatatagttg ggatataaca tcttggtttt
      781 taaagcattc ttttctaaaa acgtttcttt gtcatatctt tttatagtct cactttcaaa
      841 caaatacaga agatcagaaa tctgaagaac ctttgtttct gtattaacag gtatttcggc
      901 tatctgactg atagaatttt taagcatctc actttcctct gatacataaa gtttcacggt
      961 ttttatatct tcatttttta ctttggattt tagattgagc gctccgtctt ttttcaggta
     1021 ttcgccatac atcatctgag tatctgtaga aagaataaat ctgttttccg gaggaatctt
     1081 aacgaatgag aaaatttttt cctgagaaat ttcatttatt ccaaagttgc ttttcactac
     1141 aattttgtta tcttttacat cttcaagatt gccataaaat ctcacattat tattcattac
     1201 aaagtagtat gggagctttg gcggttctgt gccggtaccg gtaataagag taacattttt
     1261 tatatccgtt ttgaaaaacc caaaggctag tttaagatta atttcatcat taaaaaggtc
     1321 aatgctctga aaattaaggg tatacccttt agcaagcata actgaatttg gaagaacctc
     1381 ttcggttttc tgatctctta taagagaaac aacgtacttt atatcaaatt ctgcatctcc
     1441 catatcatct accttcatca gtatctttcc gttatttatg gttgtaatat caccggtcaa
     1501 aagagtattt tctccgagaa atgccttaaa tttcttgcct gaatagtcat tcgtaatttc
     1561 atctttcaga gtgttttgcc ttacattcca aaacttgagc acatttaagt ccttaagata
     1621 gaatctgcct gatgtagttg taacggtagc atcattagtt tcaagactat actctgagac
     1681 atatcctgaa aaattcattt tatctattac aacaccgttt atatcaaatg tctggtcatc
     1741 aaaagaaatc atatttatac tgtccagagg aagacttatt gctgttccaa acccttttat
     1801 actaactttt ccgtctttaa ctgatacaac agtaactttt atgctattac cgtcagtggt
     1861 ttttatagtt gcggaaaatg caaaccctgc aataatagta ataaagctta aaaatataac
     1921 caccgctttt ctcaaaacat ccacctccag cttatcttgt tttcataaag gatataatat
     1981 tttctataat tgctctgatt tcacttttta tatcgccggt ctgcaaagaa aaaccagctg
     2041 ctctaacatg tccgccaccg ccgtatttta gggctacctg tgaaacatca acgtaacttt
     2101 tcgaacgaag tgatacatgc caatcatttt tttcatattc aataaaggta actgcaactt
     2161 caacaccttt tatagatctt agctcagaca caaaaggtgg aacattaaca ggatcgaatc
     2221 catatctggt gctcatttct cttggtatgg caacataggc aagtgtatta tcgtttacta
     2281 gtttcatttc attcaacaca tccttgagaa gaagcttgct ttccaatgga acattttcca
     2341 gaatcatatt ggttatatac gaaagttctg ctttctttga cagcaaaaat gcgcaatcat
     2401 taaaaacttt ttcattaacg tttgaatatt taaaaaaacc agtatctgta gctatcccta
     2461 aaagatttgc ggtagcaagt tctttatcat attcaactcc aagacttgtt aatacttcaa
     2521 gtaccatctg cgcacatgaa gcatatgaag catctaccca attaacatga ccaaaagaaa
     2581 gatttgttac atgatgatca ataactacaa ccctcctgta actaaaaagc tgctgaacct
     2641 ttcccactct gtccggagaa gaagcatcaa ctattatcaa aagatctgcc ggatcctttt
     2701 caacacagct catttctcta attttatcat ttacaaactg aaaattcttt aagtaatcag
     2761 gtatttgtga gtctataact cctattgctc ttttgccgag tttttccaac cccataacca
     2821 ttgacgaaac agaacttaca tcatctccgt ccggatcaat atgtcctgcg acaattatag
     2881 cgcttgcctg agttatctct ttaagtattt cttctttcat cgcatttatc tcctgttaga
     2941 ataattttat tgatgttttc acaacagact caaactttcc ctttatctga tcagcaaccg
     3001 caagaacctc tttatgatta agcggttgcg gaagtatacc ggcggccata tttgttaccg
     3061 cagaaaaaga tattactttt attgaacagt gatttgcggc aataacttcc ggcatggttg
     3121 acattcctac caaatcagct ccgagtttcc gtgcaagttt tatttcagca ggcgtttcat
     3181 agctaggacc tagaaaccaa cagtaagtac cgctctttat ctctatacca gccgctttag
     3241 attggtttat aactctttct atccagttgc tatcgcacgg aaatgccatg ctcggaaatc
     3301 ttggccctat ctcatcttta ttgggaccta tcagaggatt tttaaatgaa aaatttataa
     3361 aatccctgtt tgctactata tcaccgggtt tatacatcat atttactcca ccggcagcat
     3421 tagtcaaaat aagccccttc actccaagtt ctttcattac atatatagga aatgcaatat
     3481 tacggatttt atgaccttca taggcatgaa aacgcccttt aagggttata acctgaactc
     3541 cctcaagttt tcccactaca agtttccctt catgtcctat aactgtcgaa acaggaaaat
     3601 tagggatatc tttataattt attactcttt gatcctgtat cttatctgcc aaatacccta
     3661 agccagaacc tagaatcata cctataatag gtttttcttc aatttgagag tttatatatt
     3721 cacacgctct tcttaccatg gttaacattt tcccacctct cagatctttt tattcaccaa
     3781 ttacctgcat tatataaaac ttttccttac taagatctat aattttatct tccacctgca
     3841 caactggctg aagctcgttc cctttacgta cggcaactac agtggcagtt ctggaatcat
     3901 taagcttaac catagttccg ggaggatata ctccaaagac ttttataaat gaagaagaaa
     3961 tctcaggatc aaaaattttt ccggcattct gtagaatata tgaaaccacc ttgtagtagt
     4021 tccacatgtc tttatatgct cttttggaag taagtgcatc atatacatct gctacagaaa
     4081 taagcctgcc gtaaaaagat atttccgtac cttttttacc ggctatatag ccatgaccgt
     4141 catatctttc gtgatgctca tacactcctc gtaggatatc gccttcaatc ttctgctcct
     4201 ggcaaataag cttgccaaga actacatgct gttttactat attatactca tcatcggtca
     4261 attttcttgg agcattcagt atcttttcat ctatccggct ttttcctata tcatggagaa
     4321 gccctgcata agtaagctca taaagatctt ttttcccaaa ccccatttct acagccataa
     4381 gcaaagtaat gagcccgaca tttagagtgt gcgcatatgt atactgatca aatgaattaa
     4441 gcttaacaag ggggatgtaa tttatatcac cgaatttctt cattacatct tccacaagct
     4501 cattggtaga atcagaaagc aactctttgt ctatcttttc attttgtcta agcttgtccc
     4561 atgttttctg tatgcttgaa aatgtcttct tagatattga ctgatcaagg atattatatg
     4621 atgacatctt attttcgtac gttggcacca gagctagtcc ttgataccgc aaggaatgtc
     4681 ttactgaatc agaaataatt atacccttct taagcttggc acttggaatg tccaaagcta
     4741 gtatgtattt atcaggttca atttttagtg tgtccttaaa ttttattttc atttccctct
     4801 gccacttctt ttataaaaaa tcctgtctca ggaccggttt gaatgctttt tccttccaca
     4861 tttacaaccg ggaaaacagc agctactcct ttttttataa cctgcgcaat tctgccatca
     4921 ttgagaacga ctttagtgcc gctaggatat aatccaacaa cagaaaagaa tatgcttaca
     4981 tagtaaggat cgaaaagttt tccggcattt ttcaagaggt aactcatagc ttcatatgga
     5041 ttgttaccta tttctatata tgagtcgtat gcatcgccaa tttgcattat acgcacaaga
     5101 ggacttattc tttcatctct tagacccatc agtatccctt taccatcgta cctctcatga
     5161 tgctggagaa ttgaattcac tatttccttt gaaagcgttg cttttaaagg ctcaaaagcc
     5221 ttatatccgg caattatatg agattttaca aatctatctt cgctcttatc gtaatcgaaa
     5281 ataatccgtg tattaagaaa gccgtatcca agatcatgaa taagacttgc ttttatcaat
     5341 tccagcgaaa aaactttcgg cattttaaga tgtgaggata ttattgaagc tattatagaa
     5401 gtattgatac tatgaagggt aagcgccttg tctcctatag aatggaaaag gttgagaatt
     5461 atctcatcct tttctataaa agttttgtga agatcttcta caagactttc catttcctga
     5521 ggctgttcat ttatttttgt aatgtctttt attttttcaa actgatcatt ccattcttta
     5581 tatttttttg cttctacaat cggtggaata tccttaaatt caactttaat atcttttcca
     5641 agaataaagt catcttcgtc ataaataatg acttcacgta taccacgatt tttgagtttt
     5701 tgaatatcag cctcagtaag tacattgcct tctgatagaa gaattactgc gttgtaggta
     5761 acatgttcgg caagtatgaa tccggctttt aggtcactta atttaacctt gcgcataaaa
     5821 tccccctctt aaaaagatct ctactctggc ttcaaaacag aaacatactt atttccttca
     5881 aatatatctc ctgcagccct taatacttga gataatgtta ctgactcaat attctttatt
     5941 acgtcatcaa ttgttatagg ctctttgtta ttgagataaa gatccaaaat attcatgccg
     6001 agagaaacat tactctcaat gtctattgta agttttccga tgagtctttt ctttccataa
     6061 ttaaaccatt gctctgcatc cttgtcactg taaaaactct tcagaacatt ttcaagttca
     6121 ttgttatatt tggtaagctt atctcctgaa gttgcagcaa aaaccaaaaa aactccactt
     6181 tttttataag cattgtactc tgaggtaact tcgtaaacaa gagatttttc ttctcttatt
     6241 ctgttaaaaa gcagtgaact cataccgctg ccaagaagtg tgttaagaac cagagaggga
     6301 taatatccct catcagagcg cgaaggcgct ttgaaagcgt ttactatgta atgctgtgaa
     6361 agatctttct ttgttttctc aactatgata tttttatttt taagctgcgg acttttcata
     6421 tcacttataa aattttcttt tccctcaaaa agggaaagat agtctgcaat cttatcttcc
     6481 tcgtatgaac ctgtaagtac aaccgtaaca ttggatggtt tatagaattt attataaaat
     6541 cctgtgataa ttgatttatc aaagttttca acattctttc tgttaccgat tataggacgt
     6601 gcaaactcag gattataaat atttctgaat agattttcaa agacattatc cacaggatcg
     6661 tcttcataca tcgaaatctc ttcatatatt acatcttttt ctttttctat gtctgcttca
     6721 ttaaataatg gtttaaacac aatatccgaa agtatttcca cagcagtcat tacttcagtg
     6781 gaaggtacct ttgcataata tattgtaagg ttatttccgg taaaagcatt aagagtaccg
     6841 ccaacctctt cgataggttt ctttatagat accatatccc ttgatgaagt accgcggaat
     6901 gaaacatgtt ctataaagtg tgctacaccg gaaactgttt cattctcatc ggaagaacct
     6961 gcacccacac agaatattat ggaagcgctt ctcattgaat tacgtggaac acttatgagt
     7021 ctgagattat tatttaatgt tctgatttta tacatctatt cttttgtacc tccattattt
     7081 tctttttcac gaggtacggt ttttttaaca aaaccttcct tcttaagttg gaattttccg
     7141 ttatcttcta tcgacattac ctctattttt ataaaatcac cgactttata tttcttggga
     7201 tcttctccaa ggttagaacc gtgaaggagg ccactttttc ccggagaaag ctctacaaaa
     7261 agaccatatg gctccactct gagtatctcg ccctcgataa tatctccttt cttaattatc
     7321 cttactatac tctcaatata tcttatagcg gctttcactt tctctttgtc ctgtccgctt
     7381 accttaacta taccgccttc atcctcaatc tcagtttcaa cctcaaatct tctgtttatt
     7441 tcctttatca ctttccctcc aggacctata acttcgccta ttttttctgt tgggatagcc
     7501 gttataagca tggctggaac atagggtgaa agctcttctc tagcctgagg cattgcatca
     7561 tacataaggc taagtatttt ttctcttcct attcttgctt tttcaagagc ttctcgcaga
     7621 acttcctcat ttaccttggc taccttaaca tccatctgaa aagcagttat tccatcacgt
     7681 gttccggtaa ctttaaaatc catatcgcca agatggtctt ccattccaag aatatctgta
     7741 agtacaacaa atttttcatc ttcaaatatg agacccatgg ctattcctgc cacatgtttc
     7801 tttataggta cacctgcatc catgagtgca agagatgccg aacacaccga tgccattgat
     7861 gatgagccgt tcgattcaag tatttcggat actactctta ttgtatacgg aaattctttt
     7921 tcatccggaa taagattttt gtgagctctt tctgcaagat ggccatgtcc tatttctctt
     7981 ctgcttatac ctctaagaga cttgacttct ccggttgaat aaggcgggaa gttgtaatga
     8041 agcataaatc gcttttctcc ttcggtaaat atagtatcaa caaactgaac atccattgaa
     8101 attccgagtg taacagtccc aagagattga gtttcacctc ttgtaaagag agctgatccg
     8161 tgaagccttg gtattacatg agtttcacag gttataggcc ttatttcatc aggatttctt
     8221 ccatcaactc tggtattatt ttcaataatc atctttctca tggtgttttt tacttcatcc
     8281 tcaaaaaact cgtttaaaag tcttgctttt gaagcataaa aattatctgt ccatttttca
     8341 aggtacttc
//
LOCUS       DAOSHR010000086         8238 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_7438_length_8238_cov_6.040816, whole genome shotgun sequence.
ACCESSION   DAOSHR010000086 DAOSHR010000000
VERSION     DAOSHR010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 8238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8238
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7438_length_8238_cov_6.040816"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="PLU28_05995"
     CDS             <1..194
                     /locus_tag="PLU28_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34650.1"
                     /translation="IQKHDSGNGIFYIFLDKDLGLWFDSTRNEILKLFPSICKETGLP
                     ELIIILRQKMAKYRNKILT"
     gene            complement(229..1143)
                     /locus_tag="PLU28_06000"
     CDS             complement(229..1143)
                     /locus_tag="PLU28_06000"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOG34651.1"
                     /translation="MENWEKIRAVQKMQDYILQNISEPITLLQLSRTADYSPWHSARI
                     FKELTGKTPFEYIREVRLSQAAMALRDEQAKIIDVAFDFVFDSHEGFTRAFSKQFGLT
                     PREYRRDFPPITLFMPYRVSDYYKILQKGEDTMKENPKTSTVFVQVVERPRRKAIIKR
                     GIKASHYFEYCEEVGCDIWGILTSIKDALYEPIGMWLPPSMIKPGTSSYVQGVEVPVD
                     YNGSVPQGFEVIIMEPCKMMVFQGQPYEEGDFQQAIEELWQTMKKYDPKIYGFEWADD
                     DAPRFQLAPMAYRGYIEARPVKQVNIKQ"
     gene            complement(1265..2959)
                     /locus_tag="PLU28_06005"
     CDS             complement(1265..2959)
                     /locus_tag="PLU28_06005"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOG34652.1"
                     /translation="MRKNRYVNSLRLCSQFFFVLVSTIISVTQYLAQVKLIKLPFLIS
                     LYPMTPLGTAADIFQLIKNRSIQALSQPWGIMALAVIITFVLSGSVFCGWICPFGSLQ
                     EWLGKLSKKIFPWYNKGSKKIDKYLRYLRYIVLLFVAFQTFRTSAAFFNRYDPANAIF
                     NIWSDSISMTGYGLMALFFLLSFFIERPFCRYLCPLGALGGIFNLFSLMRIKRTPSSC
                     IDCKVCDNSCAMNIRVSNESYVKDTLCNRCMKCVSNCPVNDDAVLVPQFLFSEAINHK
                     EIKKRFFIGLPVAFIIVCMIISVFIPALSHKKEKTYKNVSEITANTYLYDLFKDYQIN
                     EDALYRAFGIAYDISKNTMIKDIPLGEGVNHAFLTEKEVKNIITYLNKDIAQFVSAVG
                     RNLETLSRFTGITDFSGMTLFEVIRRSESGAIARFIYNRDIALSQKDNQAENIPSQDT
                     QEQPEFPQNNQDLQQSTVLPEDEKPLPQDGKNFYEPEQIPEPADESPTQSSTQTQQDL
                     SHDADSSQSSNTTIPTDSTYPNTPPEQSMDTAEATVLNNVEKNFFPQYTFTEIPTH"
     gene            complement(2979..3851)
                     /locus_tag="PLU28_06010"
     CDS             complement(2979..3851)
                     /locus_tag="PLU28_06010"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HOG34653.1"
                     /translation="MKAGVKKVAAIHDLSGYGRCSLSVIVPVLSTLGIQVCPVPTAIL
                     STHTDGFKDFSFLDLTDNMPGYIEHWKKIGLVFDCIYTGFLGSSKQIEIVEDFIISFK
                     TPNNFVLIDPVMADNGKLYKTMDESMVCKMRNLIGKADIITPNFTEACFLLNKKYDES
                     ISVAEIKLWMKELSDMGPGTVIITSVPVDFKKSMNTVLAYERNENKFWKVSTENVPVF
                     YPGTGDTFSSIIIGRLLCGDSLPVAIDRAVIFIKNGIKESFGFSYPTNEGILLEKVLG
                     NLNLPYITSTYEII"
     gene            complement(3913..4500)
                     /locus_tag="PLU28_06015"
     CDS             complement(3913..4500)
                     /locus_tag="PLU28_06015"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HOG34654.1"
                     /translation="MLKIGILVYDGVEELDFAGPWESMAYVNKIVEKAVKMYSIGTKK
                     EIKAYNGLKITADETIYSAPQMDILVIPGGRGRKTEMKNEETLRFIRHQYLGLKYLLS
                     VCTGAFLIAATGLLDGRSATTHKYAIQELMTYPRIKVVEKRIVKSENIITAAGITAGI
                     DLGLYMIDTIFGRDIALKISEHLEYESKELEEKSF"
     gene            4830..5489
                     /locus_tag="PLU28_06020"
     CDS             4830..5489
                     /locus_tag="PLU28_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit A"
                     /protein_id="HOG34655.1"
                     /translation="MEVLTAHGAVGLIGDDTSLMIGGFLGVGTPESIITELIAQKKKG
                     LTVIANDSAFPDKGIGRLISEQLVKKLIVSHIGTNPVTQKQMIEKSLEVELVPQGSLI
                     EKIRAGGVGLGGILTPTGVGTVVEESKQIIESEGKKYILELPLKAKFALVKAKKADYM
                     GNLFYSLTAKNFNPLIALAADIVIAEVEEIVPVGSMSPEEIHTPGILVDYVVVGGKKN
                     G"
     gene            5482..6135
                     /locus_tag="PLU28_06025"
     CDS             5482..6135
                     /locus_tag="PLU28_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HOG34656.1"
                     /translation="MDSKVLIAKRVAMELKDGDLVNLGIGLPTLVADYVPANIKVMFQ
                     SENGLIGMGPVPPQGMENPDLTNAGAVPVSSVAGAMTFDTAFSFAIIRGGHLDVTVLG
                     GLQVDEKGRLANWMVPGKMIPGMGGAMDLVTGAKKVIVAMTHTSKGEFKIIPECTLPL
                     TSIRRVDLIVTELAVIEPTDEGLVLREVAPNVTVEEVIKATQAKLIVPQTVPAMKVL"
     gene            6160..6546
                     /locus_tag="PLU28_06030"
     CDS             6160..6546
                     /locus_tag="PLU28_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold domain-containing protein"
                     /protein_id="HOG34657.1"
                     /translation="MQPVTLRLRMSQADAHYGGNLVDGARMLALFGDVATELLIRNDG
                     DEGLFRAYDSVEFLAPVYAGDYIEASGEIVTCGKSSRKMIFTARKVITARPDISDSAA
                     EVLDEPVIVCKASGTCVVPVEKQRKL"
     gene            6549..7364
                     /locus_tag="PLU28_06035"
     CDS             6549..7364
                     /locus_tag="PLU28_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HOG34658.1"
                     /translation="MEKLIITACITGAEVTRQQQPALAITPDEIAQDAYECFQAGASI
                     VHVHARNIDGSATQSLEIYRQIKEKIAEKCNIIFQPSTGGAVWHSFEERMQPLFLNPE
                     MASLSCGSCNFGDDLFVNTNEYIEKFAKEMKERNIKPEIEIFERGMIENAMRLVKKGL
                     LELPVHFDFVMNVPGAIPGTIDDLVYLRRSIPESCTWTVAGIGRTELPLAAHAIAMGG
                     HVRVGFEDNIYYSKGVLAKSNAELVERVVRLAKELERPVATPDETRQILGIRR"
     gene            7370..>8238
                     /locus_tag="PLU28_06040"
     CDS             7370..>8238
                     /locus_tag="PLU28_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-erythro-3,5-diaminohexanoate dehydrogenase"
                     /protein_id="HOG34659.1"
                     /translation="MEKGCPFGTHRVIEPKGSLPQGAKKIDNTMSIFSNEILIDVSTL
                     NIDSASFTQIKQACGNDAVKVRQMILDIVNERGKMQNPVTGSGGMLIGKVKSIGKDIK
                     DKTLKEGDSIATLVSLSLTPLKIDEILSINMQNDQVDIRGSAILFESGIYAVLPEDLP
                     QKLALAVLDVAGAPAQVDKLVKPGMNVCIIGGGGKSGVLCSYQAMKNAGSKGKVIVIE
                     YSEQNAKKIKEMNLATDVIVADATKPIDVYQKVYDLTGGQLCDVTINNVNVPGTEMAS
                     ILSTKEDGIVYFF"
BASE COUNT         2472 a         1579 c         1680 g         2507 t
ORIGIN      
        1 gtatacaaaa acatgattca gggaatggaa ttttttatat ttttctagac aaagatcttg
       61 gtctttggtt tgattctaca agaaatgaaa tattaaaact ttttccttcc atttgcaaag
      121 aaacaggact acctgaactg attattatcc tcaggcaaaa aatggctaaa tatagaaaca
      181 agattctgac gtgactattt cacgtcagaa tcttgttttc tttgttaatt attgtttgat
      241 gtttacttgt ttcaccgggc gagcctctat atatcctctg tatgccatag gggcaagctg
      301 gaatcttggt gcatcatcat ctgcccattc aaaaccgtat atcttgggat catatttttt
      361 cattgtttgc catagttctt ctatagcctg ctgaaaatcg ccctcttcat atggctgacc
      421 ctgaaaaacc atcattttac aaggttccat tattattact tcaaaccctt gcggtacact
      481 tccgttataa tcaaccggta cttccactcc ctgcacatac gatgaagtac caggctttat
      541 cattgatggg ggaagccaca ttcctattgg ttcataaaga gcatctttta tgctggtcag
      601 tatgccccat atatcgcatc cgacctcttc gcagtactca aagtaatgtg atgcctttat
      661 acctcttttt ataatcgctt tcctgcgggg acgttctact acctgcacaa aaacggtact
      721 ggtctttggg ttttccttca tagtatcttc tcctttctgc aaaattttat agtaatcaga
      781 aactcggtaa ggcataaaaa gtgtaattgg tggaaaatca cggcggtatt cacggggagt
      841 cagtccgaac tgctttgaga atgctcttgt gaatccttca tgggaatcaa aaacaaaatc
      901 aaaggctaca tctattattt tcgcctgttc atctctaaga gccatggctg cttgtgaaag
      961 tctgacctct ctgatatact caaaaggcgt tttacctgta agttctttga atatgcgcgc
     1021 actgtgccag ggagagtaat cagcagttct tgaaagctga agcagtgtaa taggttcgga
     1081 tatgttctgg agtatatagt cctgcatctt ttgtaccgct cttatttttt cccagttttc
     1141 cactttttca cctccggaat aaggatacta ttttgtatat tttttttctt gacttgaatt
     1201 gctgtcttta tcttttaaaa aagaccccac cgccattacg gccatggagt cttaatatca
     1261 gaacttaatg tgtgggtatt tcagtaaatg tatactgtgg aaaaaaattc ttttctacgt
     1321 tatttaagac agttgcttct gcagtatcca tgctttgttc tggcggagta ttcggataag
     1381 tcgaatccgt gggaatggtt gtattcgaag actgggatga atctgcatcg tgagatagat
     1441 cctgctgagt ctgtgtagac gattgggtag ggctttcatc agcaggttca ggtatctgct
     1501 ccggttcata aaaatttttt ccgtcttgag gaagtggttt ttcatcttca ggcaacacag
     1561 tactttgctg taaatcttga ttattttgcg ggaactcggg ttgttcttgt gtgtcttgag
     1621 aaggtatatt ttcagcctga ttgtcttttt ggcttaaagc tatatccctg ttatatatga
     1681 atctggctat agctcctgat tctgatcttc tgataacttc aaaaagtgtc atgcctgaaa
     1741 aatcggttat tccggtaaac ctgctgaggg tttcaagatt tcttcctaca gctgaaacaa
     1801 actgtgcaat atctttatta agatacgtta ttatattttt tacctctttt tccgtaagaa
     1861 atgcatgatt cacaccttca cccagaggaa tatcttttat cattgtgttc ttggatatgt
     1921 catatgcaat cccgaaggct ctgtaaagag catcctcgtt aatctgataa tctttgaaca
     1981 ggtcatacag atatgtatta gcggtaattt cagaaacatt tttgtatgtc ttttcttttt
     2041 tatgactaag cgcaggtata aaaaccgaaa taatcataca tactataata aaagccacag
     2101 gcagacctat aaaaaatctt ttctttatct ctttgtggtt gatagcttca gaaaacaaaa
     2161 actgcggtac aagcactgca tcatcgttga caggacagtt actgacacac ttcatgcatc
     2221 tgttgcaaag agtatccttg acataacttt catttgatac tctgatattc attgcacatg
     2281 agttgtcaca taccttacag tctatacagg aagaaggggt tctttttatc cgcataagac
     2341 tgaacagatt aaaaatacct cccaatgcac ccaaggggca taaataccga caaaaaggtc
     2401 tttcaataaa aaaactcagc aaaaagaaca gtgccataag tccataccct gtcatggaaa
     2461 tggagtcaga ccatatattg aaaatagcat ttgcaggatc gtatctgtta aaaaatgcag
     2521 ctgaagtacg gaaggtctga aaggctacaa acaaaagtac tatatatctt agatatctaa
     2581 gatacttatc tatttttttc gatcctttgt tgtaccaagg aaatattttt ttgctaagtt
     2641 ttcccaacca ttcctgcaag cttccgaagg gacatatcca gccacagaat accgaaccgc
     2701 ttaaaacaaa agttattatg accgcaagag ccataattcc ccatggctgt gataatgcct
     2761 gtatgcttct gttctttata agctgaaaga tatccgcagc agttcctaaa ggggtcatcg
     2821 gataaagaga aatgagaaaa ggcagtttta taagcttgac ctgagccaaa tactgtgtaa
     2881 ctgaaataat agtgctcact aacacaaaaa aaaattgcga acacagccta aggctgttca
     2941 catatctgtt tttccgcatt gttttccccc gatcagttct atattatctc atacgtactg
     3001 gtgatatacg gcaggttaag attacccaat actttttcaa gcaagatacc ttcatttgtc
     3061 ggataactga acccaaagct ttcttttatt ccgtttttta taaaaatcac ggccctgtca
     3121 atcgcaaccg gaaggctgtc tccgcacaaa agtcttccta taattatact ggaaaaagta
     3181 tcgccggtac cgggataaaa gactggcaca ttttcagtag agactttcca gaacttattt
     3241 tcatttcttt catatgccaa gacagtattc atactttttt tgaaatcaac aggaacactt
     3301 gttattataa cggttcccgg tcccatatca gacagttctt tcatccataa ttttatttca
     3361 gccacactta tgctctcatc atacttttta ttcaaaagga aacaggcttc tgtaaagtta
     3421 ggcgttatta tgtcggcctt gcctatcaga tttcgcatct tacaaaccat gctttcgtcc
     3481 attgttttat acagtttccc gttatcggcc ataacaggat cgatgagtac aaaattgttg
     3541 ggggttttaa aactgattat aaagtcctca acgatctcta tctgctttga agaaccgaga
     3601 aatccggtgt atatgcagtc aaaaacaagc cctattttct tccagtgttc tatgtacccg
     3661 ggcatgttat ctgtgagatc taaaaaggaa aaatccttga atccgtcggt atgtgtggaa
     3721 agaattgcag ttggaaccgg acatacctgt attcccagcg tggaaagaac tggaactatc
     3781 acagaaaggg aacatcttcc gtatccagaa agatcatgta tcgcagcaac tttttttact
     3841 ccggctttca aagtaatcga cctctgttcg tttttagtat ttaatcttat tatactatgg
     3901 atatccttca tctcaaaatg atttttcttc gagttctttc gattcgtact cgagatgttc
     3961 ggagatctta agcgctatat cgcgaccaaa aatagtgtct atcatatata atccaagatc
     4021 tattcccgcc gtaatacctg ctgcggtaat aatattttcg cttttgacaa ttcttttttc
     4081 cacgaccttt atccgcgggt atgtcatcag ctcctgaatg gcatatttat gcgtagtggc
     4141 gcttcgaccg tccagaagtc ccgtggctgc tatcagaaat gctcctgtac ataccgaaag
     4201 caggtacttc agtccaagat actgatgtct tatgaatctt aaagtttctt catttttcat
     4261 ttctgttttt cttccccttc ctccgggtat cacaagaata tccatctggg gagctgagta
     4321 tatcgtttca tcggccgtta tctttaagcc gttgtatgct tttatttctt ttttggttcc
     4381 tatactgtac atcttaacag ctttttctac tattttattc acatatgcca tgctttccca
     4441 cggtcctgca aaatccagtt cttctactcc gtcatatacg aggatgccga tctttaacat
     4501 atgactccct ccattaataa ggaatttatt gtattttttt tgagttacat tttttacatt
     4561 aatattatag ctcttttgtt ttgcttataa gttacaaaag ctatataaaa aagtacgtta
     4621 agcgtcttaa attctctctc agtcaaagtt ataatttttt gatcttctaa aggataaaag
     4681 ggcttttaag tcataaatct attataaccg cttttagagg ttacttttga tgtattttta
     4741 tttcaactgc tttatgatat aataaatttg gagattacta tgaaattttt ttcatatgag
     4801 gaagaaaatt ttataaaagg agtgatgaga tggaagtatt gactgcccat ggagcggtag
     4861 ggcttattgg tgatgatacg tctctgatga tcggcggatt tctcggggta ggtactcctg
     4921 aatctattat taccgaactg atagctcaga aaaagaaagg tttaactgta attgcaaacg
     4981 actcagcatt tccggacaaa ggcataggta ggctgatttc tgaacagctt gtaaaaaaac
     5041 ttatcgtatc tcatatagga accaatccgg tcactcagaa gcagatgata gaaaaatctc
     5101 tagaggtaga gcttgtacct cagggaagcc tgattgaaaa aatacgagcc ggcggagtcg
     5161 gattgggcgg catccttact cctacaggtg taggaactgt tgttgaagag tcaaagcaaa
     5221 taatagaatc cgaaggtaaa aaatacatac ttgaacttcc tttgaaagca aaatttgcat
     5281 tagtaaaagc aaaaaaagcc gattatatgg gaaatctttt ttattcgctt accgcaaaga
     5341 acttcaaccc tttaatcgca ttagctgcag acattgtaat tgctgaagtg gaagagattg
     5401 ttccggtagg aagcatgagt cctgaagaga tacatactcc cggtattctt gtcgactatg
     5461 tggtagtagg agggaagaaa aatggatagt aaggttctta tcgcaaaaag agttgctatg
     5521 gagctcaaag acggagatct cgtaaatctc ggaattggtc ttccgacact tgttgcagat
     5581 tatgttccgg caaatataaa ggtaatgttc cagtctgaaa atggtcttat cggtatgggg
     5641 cctgttcctc cccagggaat ggaaaaccct gatcttacaa atgccggtgc ggtacctgta
     5701 agctctgttg cgggtgcaat gacctttgat acggcttttt cttttgccat aatacgcgga
     5761 gggcatcttg atgttactgt tctgggaggt cttcaggtag atgaaaaggg aagactagcc
     5821 aactggatgg taccggggaa gatgattccg ggaatgggtg gggcaatgga tctggttacc
     5881 ggtgcaaaaa aggttatagt tgccatgacc catacttcaa agggtgagtt caaaataata
     5941 ccagaatgta ctctgccttt aacttctatc cgcagagtcg atcttatagt cacagagttg
     6001 gcagtaatag aaccaacaga tgaaggactt gtcctaagag aggttgcacc taacgtaacc
     6061 gtagaggaag taataaaggc aacccaggcc aagcttattg taccgcagac agtccctgca
     6121 atgaaagttt tataatatac agatcaggag ggatatacta tgcagccggt tactttaaga
     6181 ttaagaatga gtcaggcgga tgcccattac ggaggaaatc ttgttgacgg agcaagaatg
     6241 cttgctcttt tcggtgatgt cgccactgaa cttttgataa gaaacgatgg agatgaagga
     6301 cttttcagag cctatgacag cgttgaattt ctggctccgg tatatgccgg agattatatt
     6361 gaagcatccg gagagattgt aacctgtgga aaatcatcca gaaagatgat ttttacagca
     6421 cgcaaagtta tcactgcaag acctgatatt tccgattcgg cagcagaagt tcttgatgag
     6481 cccgtaatcg tatgtaaagc aagcggaacc tgtgtggttc ctgttgaaaa acaaaggaag
     6541 ctataaatat ggaaaaatta attataactg cctgtataac aggtgcggaa gtaacaaggc
     6601 aacagcagcc ggcactggct ataacaccgg atgaaatagc ccaggatgct tatgaatgtt
     6661 ttcaggccgg agcatctata gtccatgtac acgcaagaaa tattgacggc agtgctactc
     6721 agtcactaga aatttaccgg cagataaaag aaaaaatagc ggaaaaatgc aatatcatct
     6781 ttcagccttc aacgggcgga gcagtttggc acagttttga ggaacgtatg cagcctttat
     6841 ttcttaatcc tgaaatggca agtctctctt gcggaagctg caacttcgga gacgatcttt
     6901 ttgtaaatac caatgagtat atagaaaaat ttgcaaaaga gatgaaggaa agaaatataa
     6961 aacctgaaat agagattttt gaacgcggaa tgattgaaaa tgccatgcgc cttgtaaaaa
     7021 aaggcttact ggagcttccc gttcactttg attttgtaat gaatgtaccc ggagcaattc
     7081 ccggaactat agacgatctt gtctatctga gaagatcaat accggagtcc tgcacatgga
     7141 ctgttgccgg aatcggcaga accgaactgc cgcttgctgc acacgcaata gctatgggag
     7201 gacatgtcag ggtgggattt gaagataaca tatactactc aaagggagtt cttgcaaaat
     7261 caaatgcaga gcttgtggaa agagttgtaa gattggctaa agaacttgaa agacctgttg
     7321 caacaccgga cgaaaccagg cagattcttg gaatcaggag gtagaagata tggaaaaagg
     7381 ttgtcctttc ggaactcaca gagtaataga acccaagggc tcacttcccc aaggtgctaa
     7441 aaagattgat aatacaatga gtatattttc aaatgaaata cttatagatg tcagtacgct
     7501 gaatatagat tcggcaagct tcacacagat aaagcaagca tgtggaaacg atgcggtgaa
     7561 agtcagacag atgattcttg atattgtaaa tgaacgtggt aagatgcaga atccggttac
     7621 cggatcgggc ggaatgctta tcggaaaagt caaaagtatc ggaaaagata ttaaagataa
     7681 aactctgaaa gaaggagaca gtattgctac attggtttct ctttctctta caccgcttaa
     7741 gatagatgaa atactgagta taaacatgca aaacgatcag gttgatataa gaggatcggc
     7801 catccttttt gaaagcggta tttatgcagt tcttccagag gatctgccac agaagctagc
     7861 gttggctgtt ctggacgttg cgggtgctcc ggcacaggtt gacaagctgg taaaacccgg
     7921 aatgaacgtt tgtataatag gcggaggcgg aaaatcaggt gttctgtgtt cttaccaggc
     7981 aatgaaaaat gcaggttcaa aaggaaaggt cattgtaata gagtactccg aacagaatgc
     8041 aaaaaaaata aaagaaatga atctggcaac cgacgttatt gtagccgatg ccacaaaacc
     8101 catagatgtt taccaaaaag tctatgatct gaccggagga caactttgtg atgtaactat
     8161 aaacaatgtg aacgttcctg gcacagagat ggcaagtatt ctttcaacca aagaagacgg
     8221 tatcgtttac ttcttcag
//
LOCUS       DAOSHR010000087         8158 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_7543_length_8158_cov_4.328027, whole genome shotgun sequence.
ACCESSION   DAOSHR010000087 DAOSHR010000000
VERSION     DAOSHR010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 8158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8158
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7543_length_8158_cov_4.328027"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1007
                     /locus_tag="PLU28_06045"
     CDS             <1..1007
                     /locus_tag="PLU28_06045"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOG34660.1"
                     /translation="QDKTDRFYVVVEEYLSNFTYMITKYLSGNKLTYFIGSGREIEMI
                     ASLCQAKKDGRVLIIPKESFDKVYDYIKDKTPKQLMKIYSLSEDKAEILLPSMGIYRA
                     LLNCTDSDAIYAPIALFMDSILNEMLFPEEKQRLDKKFYESTIFCAKRIGERYFYDSG
                     HAHRVEKIAMTIFDKMKKVHGLGEKEKILLQVSSLLHDIGKYISIKKHYIHSYNIIMA
                     SDILGLNSDDLTIIANIARYHSGLLPNAEDRNFKSLSFNDRVLVAKLLSILRLADSID
                     RSHSNKFNDVQVKFQENLLDIVAYTSSDTVLEEMTFKRKSEFFEEVYGIKASFRKKVQ
                     "
     gene            1007..3115
                     /locus_tag="PLU28_06050"
     CDS             1007..3115
                     /locus_tag="PLU28_06050"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003917.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HOG34661.1"
                     /translation="MQSSLFINRELSWLEFNDRVLVEAFDKTNPLFEKLKFLSITASN
                     LDEFFMIRVSGVMEQMEDNLDSVDSSGMTAAQQMKMISQRTHELVKKQYNCLFRSIIP
                     SLEKEGITFLKMEQLDEEQKKFIYNYFKQNIFPVLTPMAVDQSRPFPLLLNKSLNLIV
                     LLEPDNTAEDKELIAFVQVPSVLPRIVQLEDKKRNLFVYLEDIMEEYISDLFPGYVLK
                     NVHTFRITRNSDLEIDEEEAKDFLIEIEKSIKRRKWGFPVRLEVEKDISGKLKHFLMK
                     KLDLDDEDVYELSNPIDLTCWMKFVSLKGFEKLRYKPMQPVMQRDLTKNQNIFDVIKE
                     KDVLVYHPFHTFDHVVEFVDKAAQDPNVLAIKQTLYRVSGDSPIVKALIKAAENGKQV
                     TVIVELRARFDEENNIQWAKKLERSGCHVVYGLVGLKTHCKVILIVRREEDGIRRYVH
                     MGTGNYNDSTAKIYTDTGLFTCKETYGSDVSSLFNVLTGYSKAPQWKKINVAPVGLRE
                     GFIELIENEINMVKQGKNGYMIAKVNAVVDEKIIEKLYEASCAGVKIDLIVRGICCLR
                     PGLKGVSENIRVISIVGRLLEHSRIFYINNGGDPKVCMSSADWMPRNLDRRVETLFPV
                     EQANLIEEVKKVLDIILSDTMKARIQKSDGTYSRIDKRGKEHIDSQAELFRYYEKQYE
                     LSVQEEIYEDAVPFINRGAE"
     gene            complement(3175..3765)
                     /locus_tag="PLU28_06055"
     CDS             complement(3175..3765)
                     /locus_tag="PLU28_06055"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOG34662.1"
                     /translation="MKVTKLVTAPIGTNTYIIDDKIVIDPGTGIGKYISSPVDVLITH
                     GHYDHISGLKELSINKVFISKPDMPMLRNPRLNASVYFDEPFSWEKECYDISDFFNTI
                     HTPGHTMGSCVILIENYLFTGDTLFSSSVGRTDLEGGSEELLKKSLDLLKVKLTEIAG
                     DYIIAPGHDMLNNEKLYTVSQILKINPFLKTKNRSL"
     gene            complement(3788..4855)
                     /locus_tag="PLU28_06060"
     CDS             complement(3788..4855)
                     /locus_tag="PLU28_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34663.1"
                     /translation="MQGWITSSQLYTIINIMDNLCGTIEPSNRKVYFYFDRGMKAFVS
                     DGCSKLLLNFSRDFLPFDGVFILPIQYLKGLLRGCSLKEDKLVNILIEDGFISFNFEE
                     MSLKAPVQESSYPPVIDTKFDVISQSSIKKFIYSCDFASAAFMEGDMITVESTGKYIY
                     FYGCGGFFQLIAKYTQTSFDFRRAIEYTTMRHVVKALQFFKKDSILSIGINEHSLLIY
                     VPGSVISICTDTPDALNTIGLENTFEETIELDSSFFKKALDKLYISFSKGVKAYFMVN
                     SERSVIYTQENDSHISFKLPYKSRNSYLIGFNSRKMRSILSRMPLSLILGLTKKEAVI
                     KDKEEVLIAKFPVQEYKLTLK"
     gene            complement(4864..>8158)
                     /locus_tag="PLU28_06065"
     CDS             complement(4864..>8158)
                     /locus_tag="PLU28_06065"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HOG34664.1"
                     /translation="EKRVELHVHSKLSSMDGTMDIQNIVSIAKEFGHKAVAITDHGVV
                     QSIPEFYDTAVSNCIKPIFGTESYVVDDFINIIKLFGPDREVDNCEYVVFDFETTGLI
                     PWENEIIEIGAVKVKNNVVINRFHSLVKPVSPVPPNIVQITGITNEMVENERKIEQVL
                     PEFLSFIENSVLVAHNADFDYRFLRVWIKKILNVDFEQTYIDTLSLSKSLLNLSGYSL
                     DKVVQALGLGDFNHHRADEDAHVTALAFIKLIGMAKTRNLTTLKDFENLKKFIDYKSL
                     RPFHMTILVKNKTGLKNLYRLISNSHIEHFYRVPRILKSELVQFREGLLIGSGCGFSE
                     MAESFIRAADRAELVERAKFYDYIELMPIEAFESGDQISAPKVAEFYRLMYDISKELG
                     IPAVMTGNVHYAEKGHKKFHDALRFNDKRQPTQALRYFRTTGEMLEQAKKIFNSDSIV
                     KEVVIENSNKIADMIEEIKPLTKKLNPPKIEGAEETVKEMSLKKAMELYGDPLPEIVK
                     KRVDRELNSIIKHGYAVLYLIAQKIVKKSNDDGYVVGSRGSVGSSLIATLMGITEVNP
                     LSPHYYCPKCKYSEFFTNGEYGSGYDLADKNCPKCGEKLLKNGQEIPFETFMGFEGDK
                     VPDIDLNFSGEYQNRAHKYIEELFGEDYVFRAGTISTVAERTAYLYAKDFAESQITVN
                     NAEIQRIASAITGVKRTTGQHPGGLMIVPRDREIFEFTPIQYPANDSSANQKTTHFDY
                     PVIHDDLIKLDALGHDDPTFIRKLYEITGIDPQTVPMDDKQTMEIFSSCRPLQIDLKK
                     ELDTSIGALGIPEFGTTFVRRMLEDTKPSSFSDLIRISGLSHGTDVWAGNAQVYIKTK
                     QATLSDVIACRDDIMISLIQKGVDNKTAFKIMEKVRKGKGIDENDESILKKVNMPQWF
                     LSSCKSIKYLFPKAHAAAYVSMAFRIAYFKVHHPLAFYATFFSVKGDEFNLAVILKGK
                     EAMRSRITFIRSQEQDPKKKNEAVVLESAIEMVLRGFSFLNVDLYKSDSFSFTLEGQS
                     LRVPFIAIPNLGQKAVEKIVTARKDGEFRSIEDLVQRTSINKTNVETLKEMGVLKGMP
                     DSNQMGLFG"
BASE COUNT         2538 a         1457 c         1594 g         2569 t
ORIGIN      
        1 tacaggataa gaccgatcgt ttttatgttg tagtggaaga gtatctcagc aacttcacat
       61 atatgataac aaagtatcta agtgggaata agcttacata ttttataggt tcaggaagag
      121 aaatagagat gatagcttct ttatgccagg ctaaaaaaga cggaagggtt ttaataattc
      181 ccaaagaaag ctttgataaa gtatacgact acataaagga taaaacgcct aagcaactta
      241 tgaaaatata tagtctgagc gaggataaag ccgagatact tctgccgtcg atgggaatat
      301 acagagcact tctgaactgt acagattcag atgcgatata tgccccgata gcacttttta
      361 tggattccat acttaatgaa atgctttttc ccgaagaaaa acaacgtctt gataagaaat
      421 tttatgagag taccatattc tgtgccaagc gtataggaga gagatacttt tacgatagtg
      481 ggcatgctca cagggttgaa aagattgcta tgacaatatt tgataaaatg aaaaaagttc
      541 acggactagg agaaaaggag aagatacttc ttcaggtatc aagtcttctt catgatatcg
      601 gaaagtatat aagcataaag aaacactata tacactctta caatattata atggcaagtg
      661 atattcttgg ccttaacagc gacgatctga cgataattgc aaatatagca aggtatcata
      721 gcggactgct acccaatgca gaagacagaa actttaaatc tctttccttc aatgacaggg
      781 ttctggttgc caagctgctt tcgattttaa gactggcaga ttcgatagac agatcacaca
      841 gcaataagtt taatgatgtg caggtaaaat ttcaggaaaa cctgctggat atagttgctt
      901 ataccagttc ggatacagtt ctagaagaaa tgacattcaa gagaaagtct gaattttttg
      961 aagaagtata cggaataaaa gcttcattca gaaagaaggt gcaataatgc aaagttcact
     1021 gtttataaat agggagctta gctggcttga gtttaacgac cgcgtgctgg tggaagcttt
     1081 tgacaaaacc aatcctttgt ttgaaaaact aaaattttta tcaattacag cttcaaatct
     1141 tgatgaattt tttatgataa gagtatcagg ggtaatggaa cagatggaag ataatcttga
     1201 ctcagtagat tcttcaggca tgactgcggc tcagcagatg aaaatgatct ctcagaggac
     1261 acatgaactg gtaaaaaagc agtataactg tcttttcaga tccataatac cttctctgga
     1321 aaaagaaggt attaccttcc tgaagatgga acagttggat gaagaacaaa agaagttcat
     1381 atacaactac tttaaacaga atatttttcc tgtgcttacc cctatggccg tagatcagag
     1441 ccggcctttt ccacttttac taaacaaaag tctcaacctt attgttcttc ttgaaccgga
     1501 taacactgcc gaagataaag aattgatagc gtttgtacag gtaccgtctg ttcttccacg
     1561 tatcgtacag cttgaagaca agaaacggaa tctttttgtt tatcttgaag atataatgga
     1621 agagtatata tctgatcttt ttccgggtta tgttcttaaa aatgtccata ctttcaggat
     1681 aacaagaaat tcagatcttg aaatagatga agaagaagcc aaagactttt taattgaaat
     1741 agaaaaatcg ataaaaagaa gaaaatgggg ctttcctgta aggcttgaag ttgaaaaaga
     1801 tataagcggt aagctaaaac attttcttat gaaaaaactt gatcttgatg atgaggatgt
     1861 ttatgaactc tcaaatccga tagaccttac ctgttggatg aaatttgtat ccctgaaggg
     1921 gtttgaaaag ctcaggtata agcctatgca gccggttatg cagcgtgacc ttacaaaaaa
     1981 tcagaatatc tttgatgtca taaaagagaa ggatgttctt gtatatcatc cgttccatac
     2041 attcgatcat gttgtcgagt ttgttgataa ggcagctcaa gatcccaacg ttttggccat
     2101 aaaacagaca ctttacagag tcagcggaga ctccccgata gttaaagctt tgataaaggc
     2161 tgcggaaaac ggcaagcagg taactgtcat agttgaactt agagctcgct ttgatgaaga
     2221 aaacaacata caatgggcta aaaaacttga aagatctggt tgccatgtgg tttacggact
     2281 ggtaggactt aaaactcact gtaaggttat tcttattgtg cgcagagaag aagacggaat
     2341 aagaaggtac gttcatatgg gtacaggtaa ttataatgac agtactgcaa agatatatac
     2401 agatacagga ttatttacat gtaaagagac ttatggttca gatgtttcat cattgtttaa
     2461 tgtgcttacc ggttattcaa aagcaccgca gtggaaaaaa ataaatgttg cgcctgtagg
     2521 tctcagagaa gggttcatag agttgattga aaatgagata aatatggtta agcaaggtaa
     2581 aaatggttat atgattgcca aggtcaatgc ggttgtagat gaaaagatta tagaaaagct
     2641 ttatgaagca tcgtgcgcag gagtcaagat agatcttatt gtaaggggaa tatgctgtct
     2701 cagacctgga cttaaaggtg taagtgagaa cattagagtt ataagtatag tgggaaggct
     2761 tcttgaacac agtagaatat tttacatcaa taatgggggc gatccgaagg tttgtatgtc
     2821 aagcgccgac tggatgccaa ggaaccttga ccgcagagtg gaaacgttgt ttcctgttga
     2881 acaggctaat cttattgagg aagtaaagaa agtactggat ataattttaa gcgataccat
     2941 gaaagcaaga atacagaaat cagacggtac atattcacgt attgacaaga gaggcaagga
     3001 acacatagac tcgcaggcag agcttttcag gtattatgag aaacagtatg agctttctgt
     3061 ccaggaagag atctatgaag atgcggttcc tttcattaat agaggggctg aataagctga
     3121 accggaaatc ttataaagag atttccggtt ctttgctatg gttttagtta ataattataa
     3181 actgcgattt tttgttttta aaaacggatt tatcttgaga atctgagaaa cagtataaag
     3241 tttttcgttg ttcagcatat catgtccggg agctataata taatctcccg caatctctgt
     3301 aagctttact tttaaaagat caagagattt tttaagcaat tcctctgagc cgccttcaag
     3361 gtcggttctt cctacagatg aagaaaataa agtatcgcct gtaaatagat agttttcaat
     3421 aagtatgacg catgagccca tagtatgtcc aggggtatgg atggtattaa agaaatctga
     3481 aatatcgtaa cactcttttt cccatgaaaa tggttcatca aagtagacag aggcatttaa
     3541 tcttggattt cggagcatag gcatatctgg tttacttatg aatactttgt ttattgaaag
     3601 ctcttttaat ccggaaatat gatcataatg cccgtgggta ataaggacat ctacaggtga
     3661 actgatatat tttccaatac cggtaccagg atcaattaca attttatcat caataatgta
     3721 agtattggtt cctatgggag ctgttacaag ctttgttact ttcaaaatat ccctccttgt
     3781 gtgggtttta ttttaatgta agtttatact cctgaaccgg aaattttgct atcaatactt
     3841 cttctttgtc ttttatgacc gcttcttttt tggtgaggcc aagtatgaga gataatggca
     3901 ttcttgaaag tattgaacgc atttttcgtg agttaaaacc tatcaaataa gagttcctgc
     3961 ttttatatgg gagtttgaat gaaatatgtg agtcattttc ttgcgtatat attacggaac
     4021 gctcggaatt aaccataaaa taagccttta caccttttga aaagcttata tagagcttgt
     4081 ccagagcttt tttaaaaaaa gaagagtcaa gttctattgt ttcttcaaaa gtattttcaa
     4141 gaccgatagt atttaaggca tctggcgtgt ctgtacatat gcttattacc gaacccggaa
     4201 cgtatattaa cagagaatgc tcgtttatac cgatgcttaa tatagaatcc tttttgaaaa
     4261 actgaagagc cttaacaaca tgacgcatgg tggtatattc tatggctctt ctgaagtcaa
     4321 aagaggtttg ggtatacttt gcgataagct ggaaaaatcc tccgcagccg taaaaataaa
     4381 tatactttcc tgtggattca acggttatca tatcaccttc catgaaagcg gcggaagcga
     4441 aatcacaaga atatatgaat ttttttatgg aagattgaga gatcacatca aacttagtat
     4501 caattaccgg ggggtaactg ctttcctgaa caggtgcctt aagactcatc tcttcaaagt
     4561 tgaagcttat aaagccatcc tctatgagaa tatttacaag cttatcctct ttcaggctac
     4621 acccgcgcag aagacctttt aaatattgta ttggaagaat aaaaacccca tcaaagggaa
     4681 gaaagtccct tgaaaaatta agcagaagct ttgaacagcc gtcgcttaca aaggctttca
     4741 ttcccctgtc aaagtaaaaa tagacctttc tgtttgaagg ttcaattgtt ccgcaaagat
     4801 tatccattat gtttattata gtatatagct gtgaagaagt tatccatccc tgcatctcac
     4861 acctcagccg aaaagaccca tttgattgga gtctggcatt cctttaagaa cacccatttc
     4921 cttaagcgtt tctacattgg ttttgtttat ggaggttcgt tggacaaggt cttctattga
     4981 tctaaactca ccatctttcc gtgcagtgac tattttctca acagcttttt gtcccagatt
     5041 aggtattgct ataaatggaa cacgtaaact ttgaccttcc aaagtgaaac tgaaagaatc
     5101 tgatttgtag aggtcgacat ttaaaaaact gaagcctctt aataccattt ctatagctga
     5161 ttccagcaca acagcctcgt tttttttctt tggatcctgt tcctgagaac ggatgaaagt
     5221 gattcttgat cgcatggctt cctttccctt aagtattaca gcaaggttga actcatcacc
     5281 tttaaccgaa aagaatgttg catagaatgc caatggatgg tgaactttaa agtatgctat
     5341 cctgaaagcc atgctgacat atgctgctgc gtgagcttta gggaaaagat actttatact
     5401 tttacacgag gaaagaaacc actggggcat attgactttc ttaagtatgc tttcgtcatt
     5461 ttcatcaata ccctttccct ttctcacttt ttccattatt ttaaaagccg tcttattatc
     5521 gacacccttt tgtataagag atatcatgat gtcatctcta caggcaataa cgtcagaaag
     5581 tgttgcctgt tttgttttaa tatatacctg ggcatttccc gcccatacgt ctgttccgtg
     5641 agaaagtcca gatattctta taagatctga aaaagatgat ggtttggtat cttccagcat
     5701 tcttcttaca aaagttgtgc caaactcagg tattcccagt gcgccaatag aggtatcaag
     5761 ttcttttttc agatctattt gtaaaggacg gcaggaagaa aagatttcca tagtttgctt
     5821 atcatccatg ggaacagttt gtggatcaat gccggttatt tcataaagtt ttcgtataaa
     5881 agtagggtcg tcgtgtccaa gggcatctag ctttataagg tcgtcatgaa ttacaggata
     5941 atcaaaatgt gtggttttct gatttgctga agaatcgtta gcaggatact gtataggagt
     6001 aaattcaaaa atttccctgt ccctgggaac gatcattaac ccaccgggat gctgaccggt
     6061 tgttcttttt acaccggtaa tcgccgaagc tatcctttgt atttcagcat tgtttactgt
     6121 tatctgtgat tctgcaaagt ctttggcgta aaggtaggct gtcctttcgg ctaccgtaga
     6181 tatggtacca gccctgaaga cgtaatcttc accgaaaagc tcttctatat acttgtgtgc
     6241 tctgttctga tactctccag aaaagttaag gtcaatatca ggtactttat ctccctcaaa
     6301 tcccataaat gtttcaaaag gtatctcctg tccatttttt aaaagttttt caccacattt
     6361 aggacagttt ttatccgcaa gatcgtatcc agaaccatac tcaccgtttg taaagaattc
     6421 agaatacttg catttagggc agtagtaatg aggactaaga ggattgacct ctgttattcc
     6481 catcaatgtt gcaattagag aactaccgac agatcctctt gatccaacca catagccgtc
     6541 atcgttggat ttttttacga ttttctgtgc aatgagataa agcactgcat aaccatgctt
     6601 tattatagag ttaagttctc tgtcaacacg tttttttaca atctccggca acggatcacc
     6661 gtaaagctcc atagcctttt tgagagacat ctcttttaca gtttcttctg caccttcgat
     6721 tttcggagga ttgagttttt tggtaagtgg tttaatttct tctatcatat cggcaatctt
     6781 gttggaattt tcaataacta cttcttttac tatagaatca ctgttaaaaa tttttttagc
     6841 ctgttcaagc atttctccag tagttctgaa ataccttaag gcctgtgtgg gctggcgttt
     6901 atcgttgaat ctgagggcat cgtgaaattt cttatgaccc ttttccgcat aatggacatt
     6961 tccagtcatt acagcaggaa ttccaagctc tttggaaata tcgtacatga gacggtaaaa
     7021 ctcagcaacc tttggagctg atatctgatc tccggattca aaagcctcaa taggcataag
     7081 ttctatgtag tcgtagaatt ttgccctttc aacaagttct gccctgtcgg cagcgcgtat
     7141 aaaagattcc gccatctctg aaaatccgca tccgctccct ataagcagac cttccctgaa
     7201 ctgtacaagt tcactcttca gtatacgcgg aactctgtaa aaatgttcta tatgggagtt
     7261 tgagattaac ctgtaaagat ttttaagacc ggttttattt ttgacaagta ttgtcatatg
     7321 gaatgggcgc agacttttat aatcaataaa ttttttaaga ttttcaaaat ccttaagggt
     7381 tgttagattt cgtgtttttg ccattccgat aagctttata aaagctaagg cggtaacgtg
     7441 ggcatcctca tctgccctat gatggttgaa atctcccagt cccaatgcct ggactacctt
     7501 atccagagag tatccggata ggttgagaag cgatttggat aaagaaagtg tatctatgta
     7561 ggtctgttca aaatctacat tcaaaatttt tttaatccaa actctaagaa atctgtaatc
     7621 aaaatcagca ttatgagcga caagaacgct gttttctata aaactcagaa attccggaag
     7681 aacttgttct atctttcttt cgttttcaac catttcattg gttattcctg ttatttgtac
     7741 tatatttggc ggaaccggag atacaggttt aacgaggctg tgaaatctgt tgataaccac
     7801 gttattttta acttttacag cacctatttc tattatctca ttttcccatg ggataagtcc
     7861 cgtagtttca aaatcgaata ccacatactc gcagttatca acttccctat cagggccaaa
     7921 taactttatg atgtttataa aatcatctac aacataagac tctgttccga atattggttt
     7981 aatgcaattt gaaactgctg tgtcataaaa ttcgggaatg ctctgaacaa caccatgatc
     8041 ggttatggct actgccttat gtccaaactc tttcgcaatt gatacaatat tctgaatgtc
     8101 cattgttcca tccattgaac taagttttga atgcacatgc agttcgactc ttttttct
//
LOCUS       DAOSHR010000088         8086 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_7645_length_8086_cov_5.738762, whole genome shotgun sequence.
ACCESSION   DAOSHR010000088 DAOSHR010000000
VERSION     DAOSHR010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 8086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8086
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7645_length_8086_cov_5.738762"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..754
                     /locus_tag="PLU28_06070"
     CDS             <1..754
                     /locus_tag="PLU28_06070"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOG34665.1"
                     /translation="LNEIILGIVQGFAEFLPISSSGHLVVFSNILNAGQNVSFFAVLH
                     LATFLAVLLFVFSDVVYLLKGLFTLDKRVLPLIVKLLLSTIPAVLTAFFLNDFINEAF
                     SSVKLVGGLFLVTALFMFFSDFFRTNKKSLDKITWIDALMIGIFQAIAILPGISRSGA
                     TLFAALFLGVKREDAVRYSFLMSLPVTFGAGVMELSKISITPAQIFGALAAFIAGLAG
                     LFLVKKLVLKGHLKIFGIYCVIISIITLVFIS"
     gene            763..1572
                     /locus_tag="PLU28_06075"
     CDS             763..1572
                     /locus_tag="PLU28_06075"
                     /inference="COORDINATES: protein motif:HMM:NF015951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HOG34666.1"
                     /translation="MLQTLFLGVLIGIANIVPGIGGGMIAAVSGRYYDILDAASNFMS
                     FKFDRKSTMLLIPVAIGMIVGIFSFSNLLELAYEKFPGYTVGTFLGLILGGLFHMGRE
                     QKVLKKDKLFLFLTGLVIAVCLFFFVIPRPDSSFQSENQSWWYLMISGMLGACAMSMP
                     AGIDGSSVLIILGVYRNAIKAISDFDFSVLIPMGLGILIGLIIIVKLLNFLMKKNKEK
                     VISVLLGVMMAGSINIFKNDISAFTGSWTVYIFVMLGFMFGFFVERLFKEK"
     gene            1694..2356
                     /locus_tag="PLU28_06080"
     CDS             1694..2356
                     /locus_tag="PLU28_06080"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG34667.1"
                     /translation="MEKVSFSYKVKKLKKNILEDFSISLQSGENLCICGDSKSGKSTL
                     LYLMSGLKKPDAGSVFFNSMALNRMEKKKLEELRQKEFGFIFQKHFLINYLSIRENIM
                     IAAVKRENESKRRLELFCEMLGITELMDTFPYQLSPVQKYRAAIARALINSPKIIFAD
                     EPMANLDCTNQRLLINLLFDYCERSGCSVIMTAMDSHTESFFDNKIHLKSLLTSAGTC
                     DD"
     gene            2349..3848
                     /locus_tag="PLU28_06085"
     CDS             2349..3848
                     /locus_tag="PLU28_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34668.1"
                     /translation="MTKNILKFFRQNFSLFSQNVLIMFISVTGAVLMNTVLTAFKTYA
                     ADAPESGSLLNMVNVFLILIIFCAAATIASIFCNKGLFWQNRKNFAVQKIMGVRNVSA
                     YAMIFSQLFIPALIGFIAGVLTGLAGTELLKNYFFEPAGIKTTLYFYTLLPAACIPLL
                     STIFSFLYGAFILHWGSPVQTIEETASFGRIKKELHLGRSSKKTFAFTIRTGTNHPVL
                     LSIFLAAVLTTLLTNITATVEMQSTQKIGEFRHITLLQPKNLGSERKISQIMSRYNIS
                     WLAAEYQIQEEKILLGTQKKYIFHANDAATRNKLRTIENLKLFSIMDNPIPAVIITVD
                     DYKKLKESDIDLSEDFEQIVDYDFLVGQTACTAYFLSLIKFLLLSLLFFILFSFIIFS
                     SAHTCRMRLGELSVMRVLGISSAKILRDTALEVSFFMILFSVGGSLSASIGWSILSNI
                     ILGERFTFYIDIAGILKIFSIGFLSYMTPLSMFFLRNDLPSMLEKCNRL"
     gene            complement(3869..5341)
                     /gene="pap"
                     /locus_tag="PLU28_06090"
     CDS             complement(3869..5341)
                     /gene="pap"
                     /locus_tag="PLU28_06090"
                     /inference="COORDINATES: protein motif:HMM:TIGR03708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0043751 - polyphosphate:AMP
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate:AMP phosphotransferase"
                     /protein_id="HOG34669.1"
                     /translation="MLENVRFQVEKEDKQVKALYEKESMRLSELQRKIKELNIPVMII
                     FEGWGAAGKGTLINELIRTLDPRGFNVFTTHLPQGEDRYWPFLYRFWIKTPARGRINI
                     FDHSWYDELFEDCVTQGKINRRILSMVDDINVFEKQLYENGELIIKFFLHISQEEQKK
                     RIKSLLRSQSEKWKVEERDLKQNENYEHYMMAFERIMEKTDTQNRWNVISSQDRKTAA
                     LQIVRTVASEFEQAVSGREASARMKENIVINSETVNSAILDSTDHTLSIPEKEYKKEL
                     KDLQMELREIQNRMYLKRIPLVIVFEGWDAAGKGGNIKRITQELDPRGYEVIPVAAPK
                     DAEREHYYLWRFWIKVPKAGHIAIFDRSWYGRVLVERVEGFCSVQEYKRAYKEINDFE
                     SQLVNYGATVVKFWLDISKEEQLKRFNDRQNDPGRSWKITDEDWRNREKWDLYKPAVE
                     EMLLRTSTSYAPWTVVESDDKYYARLKTLRTVVQSMKKKI"
     gene            5503..6099
                     /locus_tag="PLU28_06095"
     CDS             5503..6099
                     /locus_tag="PLU28_06095"
                     /inference="COORDINATES: protein motif:HMM:NF017530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPP34 family phosphoprotein"
                     /protein_id="HOG34670.1"
                     /translation="MPESFFLICINNEDHSVKKKLGGLFDFGLSAAVLSEMLLKDYIS
                     LVDKLAVTFDDKKTGEDLYDFFLEKIHAEKKIHSLKYWIKKAGKKRKFIQNEVAAGLE
                     GKNIIEPIGKKEYIIKNTAIVQNIYKNIRITAQKSTSIDERSVILLSLAKSCKLLKDI
                     LGSDEKQELKEDIKDIIKTDFFSNKLFEILQELEELED"
     gene            6428..6982
                     /locus_tag="PLU28_06100"
     CDS             6428..6982
                     /locus_tag="PLU28_06100"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOG34671.1"
                     /translation="MARRPNPDLRERRQQELIESIISIINENSISGTTTRKIASEASL
                     TIASLHYYFGSKDGAMIETAAFILQEWIQPILEKPEDIEEKINKLFIPSDKMAAFAQI
                     MTYPYRSKVTIRKIQEIDEKFSLLIKDVLRANGFNVDSSFIIADVLKTFLIGLGFKGY
                     VYPETIKDEINTVINFLNLRKVRE"
     gene            6984..>8086
                     /locus_tag="PLU28_06105"
     CDS             6984..>8086
                     /locus_tag="PLU28_06105"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF003766.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HOG34672.1"
                     /translation="MRVILVILDSVGIGEMPDAPLYGDTGSNTLANIARSVNGLRMPN
                     MQKLGLGNIYPIQGVFPVQASLGAYGKLKEFNDGKDTTTGHWEMSGIYLEKPFDFFPN
                     GFDSHIINEFELRTNRKVIGNTVASGTKIIQDLGPEQEKTGSWIVYTSADSVFQVAAH
                     EEIIPLEELYHGCKIAHEMFTEMGYNIGRIIARPYIGKYPEYIRTSNRKDISSPPPAE
                     TLLDILTDSGIKVYAVGKIHDIFAERGISEFVKTVDNIDGIEKTLLYMKEKNENCFIF
                     TNLVDFDMKYGHRNDVQGYANALMQWDEELISIMSQMKNDDILMISADHGCDPTTPST
                     DHSREYIPFLAYGNPVKENVNIGTRESFADIGQT"
BASE COUNT         2410 a         1560 c         1487 g         2629 t
ORIGIN      
        1 tttgaatgaa attattttag gcatagttca ggggtttgcc gagtttctac ctatttcaag
       61 ctccggtcat ctggtcgttt tttcaaatat tctcaatgcc ggtcagaatg tctctttttt
      121 tgctgtactt catcttgcaa cctttctagc ggtgctgctt tttgttttca gcgatgtagt
      181 atatctcttg aaaggtcttt tcacactcga taagcgtgtg cttcctctca tagttaagct
      241 tttgctttca actattccag ccgtacttac cgcattcttt ctcaatgatt tcataaatga
      301 ggctttttct tcagtaaagc ttgtaggagg gctcttcctc gtaactgcgc ttttcatgtt
      361 tttttctgat tttttcagaa ccaacaaaaa aagtctggat aaaataacct ggatagatgc
      421 tcttatgatt ggtatttttc aggctatagc catactgccc gggatttcaa ggagcggggc
      481 tactttattt gccgctttgt tccttggagt aaaacgcgag gatgccgtaa ggtactcttt
      541 tcttatgagt cttccggtaa cttttggagc tggagtcatg gagctttcaa agatctcaat
      601 cacaccggct cagatttttg gagcgttggc cgcttttatc gctggcctgg caggactttt
      661 ccttgtaaaa aaacttgtgc tgaaaggtca tttaaagatt ttcggaatat actgcgttat
      721 tatttcaata atcaccctag tctttatttc atgaggtgat tgatgcttca aactttattt
      781 cttggagtgc ttataggaat tgccaatatt gtccccggaa taggaggcgg aatgatcgcc
      841 gccgtttccg gaaggtacta cgatattctc gatgctgctt caaacttcat gtctttcaaa
      901 tttgacagaa agtcaaccat gctccttatt ccggttgcaa taggaatgat tgtgggaata
      961 ttttcttttt caaatcttct ggaactcgcc tacgaaaagt ttccaggata tactgtgggc
     1021 acttttttgg gcttaatact gggtggactt tttcacatgg gtagagaaca gaaggtgctc
     1081 aaaaaagata agttgttcct ttttctgaca ggtctcgtta tagctgtatg tcttttcttc
     1141 tttgtaatac cacgcccgga cagttccttc caaagtgaaa atcagtcatg gtggtatctc
     1201 atgataagcg gaatgttagg agcatgcgca atgtcaatgc ctgccggaat agacggttct
     1261 tcagtactga taattcttgg cgtatacaga aatgccataa aagctatctc agactttgat
     1321 ttttctgttc ttataccgat gggtctggga atccttatag gccttattat tattgtaaag
     1381 cttttgaact ttcttatgaa gaagaacaaa gaaaaggtaa tctctgttct tctcggagta
     1441 atgatggcag gaagtataaa tatattcaaa aatgacatct ccgccttcac aggaagctgg
     1501 acagtatata tatttgtaat gcttggcttt atgtttggtt tttttgtgga aagacttttt
     1561 aaagaaaaat aattacataa gtaatgcaac ttcatgactt agttcattaa aagaactagg
     1621 tcatttttta gtataatcat cattggtata ctgcacctgc ttaccggaaa gaggggagat
     1681 atatttgctt aggatggaaa aagtatcttt ttcatacaaa gtcaaaaaac ttaaaaaaaa
     1741 tatccttgaa gatttttcaa tttcattgca gagcggtgaa aatctctgta tctgcggtga
     1801 ttccaaaagt ggaaaatcta ctcttcttta ccttatgtcc ggattaaaaa aacctgatgc
     1861 tggaagtgtt ttttttaaca gtatggcgct taaccgcatg gaaaagaaga agcttgaaga
     1921 actgcgacaa aaggagttcg gcttcatatt tcagaagcat ttcctgataa actacctttc
     1981 tatacgtgaa aacattatga ttgcagccgt gaaaagagag aatgaaagta aaagacggct
     2041 tgaacttttc tgtgaaatgc ttggaataac agagcttatg gatacttttc cgtatcagct
     2101 ttcacccgta caaaagtatc gggcggctat tgcaagagct ttgataaact ctccaaaaat
     2161 aatctttgcc gatgaaccta tggcaaacct cgactgtaca aatcaaagac tgttgataaa
     2221 tcttctgttt gattactgcg aaagatcagg ctgttcagtt attatgactg ccatggattc
     2281 tcatactgaa agcttttttg ataataaaat acatctgaaa agccttctta cttctgccgg
     2341 gacttgtgat gactaaaaat attctgaagt tcttcagaca gaatttttca cttttttctc
     2401 agaatgttct tattatgttc atatctgtta caggagcagt tcttatgaat actgtcctta
     2461 cagcatttaa aacttatgct gccgatgctc cagaaagcgg atcactcttg aatatggtga
     2521 atgtttttct tattttaatt atattctgtg cagctgcaac tattgccagt atattctgta
     2581 ataaaggact tttttggcaa aacagaaaga actttgcagt tcagaaaatc atgggagtaa
     2641 gaaatgtttc agcttacgcc atgatttttt cgcagctctt tattcctgct ctgattggtt
     2701 ttattgccgg agtacttacc ggattggccg gaacggaact tttgaaaaat tatttttttg
     2761 aaccggcagg gattaaaacc actttatatt tttatacctt gctgcctgcg gcatgtattc
     2821 ctcttttatc aaccatcttt tcttttttat acggtgcttt catcttacat tggggtagtc
     2881 ccgttcagac aattgaagaa acagcttcct tcggtcgaat taaaaaggag cttcatttgg
     2941 gaagaagctc aaaaaaaaca tttgctttca ccatacgtac tggcacaaat catccggtgc
     3001 ttttaagtat ttttctggct gcggttttaa caaccctgct gacaaacatc actgcaactg
     3061 ttgagatgca gtctacacag aagataggtg aattcaggca tattactctt cttcagccca
     3121 aaaatcttgg ttctgaacgg aaaatatctc aaatcatgag caggtacaac atctcctggc
     3181 ttgcagcaga gtatcagatt caggaagaaa agatcctcct gggaacacag aaaaaataca
     3241 tattccatgc aaatgatgct gccacacgca acaagctgag aacaatagaa aatttaaaac
     3301 tcttttcgat aatggacaac cccatacctg cagtaataat aacggttgat gactacaaaa
     3361 agctgaaaga atccgatata gatctttcag aagactttga acagatagta gattacgatt
     3421 ttcttgtagg tcagacagct tgcactgctt attttttaag tcttataaag tttttattac
     3481 tatccttgct tttttttatt ctcttttctt ttataatttt ttcttctgca catacttgcc
     3541 ggatgcgtct tggtgaactt tcggttatgc gtgtcctagg aatatcttct gccaaaattt
     3601 taagggatac tgccttggaa gtatctttct ttatgatact tttttctgta ggcggttcgt
     3661 tatctgcgag tataggatgg agcatactct caaatattat tttgggagaa cgtttcactt
     3721 tttatattga tattgcaggt attttaaaaa ttttttcaat aggattttta agttatatga
     3781 ctcctttatc catgtttttt ttgagaaatg atctgccttc tatgctagaa aagtgtaacc
     3841 gtttataaca aaaaggttta gtcatcattt atatcttttt cttcatcgat tgcactaccg
     3901 ttctgagagt cttaagcctt gcatagtatt tatcatctga ttcaacgacg gtccagggtg
     3961 catatgaagt gcttgtcctt aaaagcatct cttctactgc aggtttataa agatcccatt
     4021 tctccctatt tctccagtct tcatcggtaa tcttccagct tctgccaggg tcgttctgcc
     4081 tatcgttaaa tcttttcagc tgttcttctt tacttatatc aagccagaat ttcacaacag
     4141 ttgctccgta atttacaagc tggctttcaa aatcattgat ttctttatat gcccttttat
     4201 actcctgcac cgaacagaag ccttcaaccc tttcaaccag aaccctgcca taccatgacc
     4261 tatcaaaaat tgctatatgc ccagctttag gtacttttat ccaaaatctc caaagatagt
     4321 agtgttctct ctctgcatcc ttcggagcag ctactggaat tacctcatag cctctgggat
     4381 caagctcctg ggttatacgt tttatattgc cgcctttgcc tgcggcatcc cagccttcaa
     4441 aaacaatcac aagcggaata cgttttaggt acattctgtt ttgaatctcc cgcagttcca
     4501 tctgaaggtc tttcaactct tttttatact ccttttcggg tatactcagc gtgtggtccg
     4561 tactgtccag aatggcagag tttacagttt cagagttgat aactatgttt tccttcatcc
     4621 gagcagaagc ttctctgcct gaaacagcct gttcaaactc tgaagcaaca gtccgtacta
     4681 tctgaagcgc tgcagttttt ctatcctgcg aagagattac attccatctg ttttgggtat
     4741 ctgttttttc cattattctt tcaaatgcca tcatatagtg ttcataattt tcattctgtt
     4801 tgagatctct ttcttctact ttccactttt cactctgact gcgcaaaagt gattttatcc
     4861 ttttcttttg ttcttcctga cttatgtgca aaaaaaattt tattataagc tctccgtttt
     4921 catagagctg cttttcaaag acatttatat catccaccat actcaaaatc ctgcggttta
     4981 tctttccttg cgtaacacag tcttcaaaca gctcgtcata ccagctgtga tcaaaaatat
     5041 ttattctgcc ccgtgcaggt gtttttatcc agaacctgta taaaaaaggc cagtatctat
     5101 cttcaccttg tggaagatgt gttgtaaaaa cattaaatcc cctcggatca agcgtcctta
     5161 taagctcatt tatcagagta ccctttcctg ctgcacccca tccttcaaaa attatcatga
     5221 caggaatatt cagttccttt atcttccttt gaagttcgga aagtctcatg ctttcttttt
     5281 catacagagc tttgacctgt ttgtcttctt tttccacctg gaaccgcaca ttctcaagca
     5341 tacttcacct cccggcattc tttgcttagc acctctctta tttatccaat tataacatat
     5401 tatacatact gcttttttat taagaaatat taagaggatt gaaaaacatc cccaaagcat
     5461 agtatcatta tagtggcttc aggaggtggt actatattat ctttgcctga gagtttcttt
     5521 ttaatatgta taaataatga agatcattcg gtaaaaaaga agttaggagg gctttttgat
     5581 ttcggccttt ctgcggcggt attgagtgaa atgctgctta aggattatat atctctcgta
     5641 gataagcttg ctgttacctt cgatgacaaa aaaacaggcg aagacctgta tgactttttt
     5701 cttgaaaaaa tccatgctga aaaaaaaatc cacagtctca agtactggat taagaaggct
     5761 ggtaaaaaaa ggaagttcat ccaaaatgaa gttgcagccg gtctggaagg aaaaaatatt
     5821 atcgaaccca ttgggaagaa agaatatatc ataaaaaaca cagccatcgt acagaatata
     5881 tacaaaaaca tccgtattac tgcacagaag agcacctcaa ttgacgagcg ctcggttatt
     5941 cttctatcac tcgccaaaag ctgcaaactt ttaaaagata tcttaggaag cgatgaaaaa
     6001 caagagctga aagaagatat taaagatatt ataaaaactg attttttcag caataagctt
     6061 ttcgagatac ttcaggaact ggaagaattg gaagattaaa ttaatgtctg atcaaatccg
     6121 aggccttgca ggccttattt tttttctggg aaaatagtta aaaaaacgta tattatgaat
     6181 taactgtatt ttttttggat aaaatattct tgcacttttg tacttttatg atataatttt
     6241 attactcaaa ttcctaagta catttgtcaa acttatccat aatgatatat aatcttttcg
     6301 aatatatgaa cataatataa atacactgaa aaatcaaaaa aatcactttg tttatgtagt
     6361 atacttgtaa aaatatctgt gatataataa tttcataaga tgattttcat acaaaggggg
     6421 actgactatg gcgagaagac ccaacccgga cctgagagaa agacgccagc aggagctgat
     6481 agagtcaatc ataagcataa tcaacgaaaa cagcataagc ggaaccacaa ctcgcaaaat
     6541 agccagcgaa gccagcttga caattgcttc gctacactat tactttggct caaaagacgg
     6601 cgccatgatc gaaacagcag cttttatact tcaggaatgg atacagccca tacttgaaaa
     6661 accagaggat atcgaagaaa agataaacaa gcttttcata ccgtccgata agatggctgc
     6721 ttttgcccag ataatgacct acccttacag atccaaagta accataagaa aaattcagga
     6781 gatcgacgaa aagttttcac tgcttataaa agatgtactg agagccaacg ggttcaatgt
     6841 agactcatct tttataattg ccgatgtgct caaaaccttc ctcataggtc ttggcttcaa
     6901 gggttatgtc tacccggaaa ccataaaaga tgaaataaat accgtcatta acttccttaa
     6961 tctgcgaaaa gtgagggagt agtatgaggg taattcttgt catacttgac agcgtcggaa
     7021 taggcgaaat gcctgatgct ccgctatacg gagacacagg ttccaatact ctggcaaata
     7081 tagcaagatc agttaacggt ctgagaatgc ccaatatgca aaaactcgga ttgggaaaca
     7141 tatatcctat acagggagtt tttcctgtgc aggcaagtct tggagcctac ggaaagctca
     7201 aagagttcaa cgatggaaaa gatacaacca ccgggcactg ggaaatgagc ggaatctatc
     7261 ttgaaaaacc ctttgatttt ttccctaacg gatttgacag tcatataata aatgagtttg
     7321 aactcaggac aaaccgcaag gttataggca atactgttgc ttctggaact aaaataattc
     7381 aggatctcgg acctgaacag gaaaaaacgg gttcatggat tgtttatact tccgcagaca
     7441 gtgtttttca ggttgctgcc cacgaagaaa taattccgct ggaagaactt taccatggct
     7501 gcaaaattgc tcatgaaatg tttacagaaa tgggttacaa tatcggaaga ataatcgcca
     7561 gaccatatat tggaaagtat ccagagtata taagaacttc aaacagaaaa gatatttctt
     7621 ctccaccgcc tgcggaaaca ctacttgata ttcttacaga ttctggaata aaagtgtatg
     7681 cagtgggaaa gatacacgat atttttgccg aaagaggtat ttcagagttc gttaaaaccg
     7741 ttgacaatat cgacgggatt gaaaaaactc ttttatatat gaaagaaaaa aatgaaaact
     7801 gctttatttt taccaatctg gttgattttg atatgaagta tgggcacaga aatgatgttc
     7861 agggttatgc aaacgctctt atgcaatggg atgaagaact tatatctata atgtctcaga
     7921 tgaagaatga tgatatcctt atgataagtg cggatcatgg ctgcgatcct acaacccctt
     7981 ccacagacca ctcacgggaa tatatacctt ttctggcata cggaaaccct gttaaagaaa
     8041 atgtaaacat cggaacaagg gagtcctttg ctgatatagg tcaaac
//
LOCUS       DAOSHR010000089         7995 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_7753_length_7995_cov_5.158816, whole genome shotgun sequence.
ACCESSION   DAOSHR010000089 DAOSHR010000000
VERSION     DAOSHR010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 7995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7995
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7753_length_7995_cov_5.158816"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1493)
                     /locus_tag="PLU28_06110"
     CDS             complement(<1..1493)
                     /locus_tag="PLU28_06110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOG34673.1"
                     /translation="MDFYKKGMLPLKVSFFMLTIFLIISGSFFSFWTIKLADEKMRAD
                     LLLQAEIVSRTINISDIKALDGNLQDVTLLEYRCLKNQLTDICSVIPNCRFAYILGKK
                     ENGNEYNVVDSEKISSQFYDPPGHEYDEPQEIDEKVFKTGIAQTDGPISDSAGTYITA
                     LVPIKNPDSNDILVLLGLDIDTKGWSQNLLKQTWLSVALIFLMEIVLIVFWYIHLQSK
                     KLRQSEDRLKTIADSVTDGIIMTDSNGAIAFWNKGAEKIFGYSTVDASEKKIYELIKD
                     SNAILKFKNLIFDYLLQGKEFSSLELETFGKRKDGKEVLLSVSLSKIIFEKKHYFVGI
                     LKDITQAKITRDTLEKIIDNNPISIQLTDKNGYCIRTNPAHLRLFGAVPSETYSVFSD
                     PVIKESGLENLMEKVQNGEELVFPEILYNANKINPRFPDKPVWIKAIVFPLMGSDNKP
                     EKYVIMHQDITQERKKQKDIEYLSFHDQLTGLYNRRFFETELAVLDEP"
     gene            complement(1523..2539)
                     /locus_tag="PLU28_06115"
     CDS             complement(1523..2539)
                     /locus_tag="PLU28_06115"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOG34674.1"
                     /translation="MKEKINFSSSAVDSLCEEKEFSGVIQIDEENSTVYSKAFGYADI
                     IEKRKNNMDTLFAIASGTKFFTALGIGILIDRGLFTLDTPAKELFDLNIKTYSDNITI
                     RHLLSHTSGMPDYYIEEDTEKLNDFEPEYPFYKLVSPKDYLVLFPKEPMKFQPGERFE
                     YNNSGFVYLAMIIEHISGKSYKEFIEEEIFRKNGLTRSGIFPFNALSPNTAWGYTIEC
                     DSWKSNIYKLPIQAGGDGGIYICAGEMKLIWEYFFKNKIISADLVNEFITPQTAVPVE
                     NDDYYGLGLWLRPKKAYLIPYIVGSDPGISFISSYDPYKISYTFLVSNTTDGVWNVIS
                     GIRQ"
     gene            2679..3086
                     /locus_tag="PLU28_06120"
     CDS             2679..3086
                     /locus_tag="PLU28_06120"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOG34675.1"
                     /translation="MDRILIKEDIIPETEELLKLYNDAQWSAYTNDSYRLKNAFRNSL
                     KVFTVWDTDKLIGLIRVVGDGQTIIYIQDILILKKYQRQGIGSKLLQLVLERYKSVRQ
                     VVLMTDNTEGTIDFYKKNGMVKASEHNCIAFIK"
     gene            complement(3182..4963)
                     /locus_tag="PLU28_06125"
     CDS             complement(3182..4963)
                     /locus_tag="PLU28_06125"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HOG34676.1"
                     /translation="MEDFMSYVNYQRINECIELLSIMTKLPLGIMDCSGKLLFSHGIK
                     GIIIDNINMSLQESFLLSEYSFIPGEKSRVCLLPISTGKKIQAYLIRIISEEIEKDTV
                     KKVKFLEYLRDFISCNLFCKREQNEMNNQFNFIFETDTCLSEKKRLAHDYKEQNSRLS
                     YFDSLHNCMPNRNYLKVFGEELTHCSKPFTLLYLDVSDIKVVNDTIGYEAADVWLKQT
                     MEKLMGSINSTEKVFRWKDDAFVFILEKTELLYIQKTAERLLNIVEESFEISGVKIRI
                     LGYIGAVAYPEDGTSLEELIRKASVAVRTSQIKGNRKFNYYDEKMAKSIIEKLEIEKD
                     FEKALSEKEFFLCYQPVFDAKNNVLYCFEALIRWKHPAKGVLFPATFIPVAEETGFIE
                     SIGGWVIEEVFAKVQELSKLGYDNLKFSINISVRQLGNRKFLEYTELCMKKYSINPEN
                     IILEITETVMINGFSNNINEIFFQLKKMGFKIALDDFGSGYSSISYLKNIPVDIIKID
                     KIFIDKISEEGVEKLFIKTALAIGEKLGKQIIAEGVESEKQKKLLLLEGCRLLQGYIF
                     SKPIKDGELLVYLDHARKTSEITGCNP"
     assembly_gap    5270..5369
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5370..5730
                     /locus_tag="PLU28_06130"
     CDS             <5370..5730
                     /locus_tag="PLU28_06130"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOG34677.1"
                     /translation="YMNTGEKIKNLREEKGLSREKFGALINIAGNSVYRIENGFNLPD
                     AKTLLNISKTLDVSIDYLLGLSEVRTIQKENVDKIPYDIKQKLLKLERIHNQNLPEKL
                     ERIGVELIEISKLLKEI"
     gene            5909..6046
                     /locus_tag="PLU28_06135"
     CDS             5909..6046
                     /locus_tag="PLU28_06135"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOG34678.1"
                     /translation="MFSDKLKELRQKNNLTQEQLAQKINTTRKNISAYETGRSAPMHR
                     T"
     gene            6050..6229
                     /locus_tag="PLU28_06140"
     CDS             6050..6229
                     /locus_tag="PLU28_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34679.1"
                     /translation="MIADIFNVSTDYLLERSIPENIMLNTLTGQNVPDTVLQKLLKLD
                     RIETENFPIGWKDSI"
     gene            complement(6335..6943)
                     /locus_tag="PLU28_06145"
     CDS             complement(6335..6943)
                     /locus_tag="PLU28_06145"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOG34680.1"
                     /translation="METDSFEKESLLYETALKEFSEKGYKNASLNSILKNTGISKGTF
                     YYHFQNKEELYICLVRKALEIKKQLLEKSFSENGLECDIFLLLQMQAETVIEYAFNHR
                     LFYDFSVSFAKEKGNSIYEKVMNSFKPEIDKTFSKIINIAAESGTLRDDIPMEVLERS
                     LKYFFTNLSDILELFYKVKDKKKLLEYYKSFIEILKNGMSKH"
     gene            complement(6949..>7995)
                     /locus_tag="PLU28_06150"
     CDS             complement(6949..>7995)
                     /locus_tag="PLU28_06150"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG34681.1"
                     /translation="IFSGNILFCKENSNKKGDFMRIVALSKKDISNIKNDKMMIFMFL
                     IPLVIAFAVRLMIPSISSGTTKIFVSQNLPVEYEKKLASYFEVIKTTDRKNFENSIMA
                     FTDIPGIDFKDNSLEVVLQGDEEPGIIRILNAVIHDIEKGSPQVPVTDIGTKKSGISE
                     LIISMILIFVISISGVICGFTMVDDKESGMINALAVSPVRLSDYITSKVIVAVSYSII
                     VSFLSQLIMTGFQFSIKNYLLVILSGMLLSLTVGFIIGTLADTQNMAIAYTKIVTFIL
                     VFVPMLSLLLPSSIQWVFGIIPSYWTFNIIWQLYLGIDGSIFLSSVISVVYNTLLLVL
                     LSSKLKRKIGLRLG"
BASE COUNT         2582 a         1343 c         1236 g         2734 t
ORIGIN      
        1 ggttcatcca aaactgcaag ctctgtttca aagaatcttc tgttatacaa accggtaagc
       61 tgatcatgaa agctaagata ttcaatatcc ttctgctttt ttcgttcctg tgtgatatct
      121 tggtgcataa tcacatattt ttcaggtttg ttgtcactcc ccataagagg aaatactata
      181 gcttttatcc atacgggctt atctggaaat cttggattga ttttgtttgc attatagagt
      241 atttcaggaa aaacaagttc ttctccattt tgaacttttt ccattaggtt ttccagtcca
      301 gactctttta tgacaggatc agaaaaaacc gagtaggttt ctgaaggtac tgcaccaaaa
      361 agccttaaat gtgcaggatt tgtccgtata caataaccgt ttttatctgt aagctgtatt
      421 gaaataggat tattatctat tattttttcc aaagtatctc gcgtgatttt agcctgtgtt
      481 atatctttta atattcctac aaaataatgt tttttctcaa aaataatctt tgaaagagaa
      541 accgaaagaa ggacttcttt gccatccttt ctttttccaa atgtttcaag ttctaatgaa
      601 gaaaattctt ttccttgaag aaggtaatcg aatatcaggt ttttgaattt taatatggca
      661 ttggaatctt tgatcagttc ataaattttt ttctctgaag catcaacagt actatagcca
      721 aaaatttttt ctgctccctt gttccaaaaa gcgattgctc cgttactatc ggtcatgatg
      781 ataccgtcag ttacagaatc tgcaatagtt tttagcctgt cttcgctttg cctgagtttt
      841 ttactctgca gatgaatata ccagaatact atgagaacaa tctccataag aaatataagg
      901 gctactgaca accatgtctg ttttaggaga ttttgagacc agcctttggt atcaatatca
      961 agaccaagaa gtacaagaat atcattagag tctggattct ttatcggcac cagtgccgtt
     1021 atatacgttc ccgcagaatc ggagattgga ccatctgtct gggctattcc ggttttaaag
     1081 actttttcat ctatttcctg cggttcatcg tactcatgac ctggaggatc atagaactga
     1141 gagcttattt tttcagaatc tacaacatta tactcattcc cgttttcctt ttttccaagg
     1201 atataagcaa accgacagtt tggtattaca ctgcatatat ctgtcaactg attttttaag
     1261 catctatact ccaaaagagt aacgtcttga agattgccgt caagagcttt tatatcagat
     1321 atatttattg ttcgtgagac aatttctgcc tgaagtaaaa gatcggctct catcttctca
     1381 tcagctaatt ttatggtcca aaaagagaag aaagatcccg atataataag aaagatagtg
     1441 agcataaaaa acgagacttt taaaggcaac attccctttt tataaaagtc catccggttt
     1501 tctcctttat attagagatt ttctactgtc taatgcctga tatcacattc cataccccgt
     1561 cagttgtgtt tgaaaccaag aatgtatagg agatcttgta aggatcatag ctgcttataa
     1621 aagagattcc gggatcagat cctactatgt agggaataag ataagctttt tttggtctaa
     1681 gccacaatcc aagtccataa taatcatcat tttcaaccgg tacagcagtt tgtggagtaa
     1741 taaattcgtt taccagatca gccgagataa ttttgttttt aaaaaaatat tcccatatga
     1801 gtttcatttc tccggcgcat atataaattc cgccatctcc gccagcttga ataggaagct
     1861 tgtatatatt gcttttccaa ctatcacatt ctatggtata accccaggct gtattaggac
     1921 tgagcgcatt aaagggaaat atgccgctcc tggtaagacc attttttcga aagatctctt
     1981 cttctataaa ctccttataa ctttttccac tgatatgttc tattatcatg gcaagatata
     2041 caaaaccact gttattatat tcaaaccttt cacctggctg aaacttcatg ggttctttag
     2101 gaaaaagcac taaatagtct ttaggtgaaa caagtttata aaaaggatat tcaggttcaa
     2161 aatcattaag cttttctgta tcctcctcga tgtagtagtc cggcatccct gatgtgtgag
     2221 aaagcagatg tcttatggta atattgtctg agtatgtttt tatattaagg tcaaaaagtt
     2281 ctttggcagg agtatcaaga gtaaataagc cacgatcgat gagtatacca attcccaagg
     2341 ctgtaaaaaa tttcgttccc gaggcaattg caaataaagt atccatatta ttttttcttt
     2401 tttcaataat atcggcataa ccaaaagctt ttgaatatac tgtgctgttt tcttcgtcga
     2461 tttgtatgac tcccgagaat tctttttctt cgcagagact gtcaacggct gaagatgaaa
     2521 aattaatttt ttctttcatt tgtacctccg ttgggtaaaa tagatactta actcttaatt
     2581 tgcagtttgt acaataaatt atacaattat ttagtgaatt ttaaaagtat tttctcttat
     2641 taagaataaa acactttata aaaattaagg aggatattat ggatagaata ttgattaaag
     2701 aagatatcat acctgaaact gaagaactgc ttaaattata taatgatgca cagtggtcag
     2761 catatacaaa cgacagctat agattaaaaa atgccttcag aaattcatta aaagtattta
     2821 ctgtatggga tacagataag ttaattgggt taatccgagt agtaggagac ggtcagacta
     2881 ttatttatat tcaggatata ttgattctaa aaaaatatca aagacagggt attgggagta
     2941 agcttttaca gttggtttta gaaagataca aatcagttcg tcaggtagtt ttaatgacag
     3001 acaataccga aggaacaata gatttttata aaaaaaacgg tatggtaaaa gcgtctgagc
     3061 ataattgcat tgcatttata aaataggtaa cttacaaaac taaaagatgc ataaataaaa
     3121 aataaaacat cttctaaccg aagatgtttt tataatataa aatgtaactt ttattctttt
     3181 attatggatt acagcctgta atttcagaag ttttgcgggc atgatccaga taaacaagca
     3241 gttcaccatc ttttataggt ttgctgaata tataaccttg gagcaacctg cagccttcaa
     3301 gtaagagcag ttttttttgt ttttcagatt caactccttc tgctattatt tgttttccta
     3361 gcttttcacc tattgctaaa gctgttttta tgaaaagttt ttctacacct tcttcagata
     3421 tcttatctat aaaaatttta tcgattttta ttatgtcaac aggaatattt ttgagataac
     3481 ttatgcttga atatccagaa ccgaagtcat caagtgcaat tttaaacccc atttttttaa
     3541 gttggaaaaa gatctcgtta atgttatttg aaaagccatt gatcataact gtctctgtta
     3601 tttcaagaat aatattttca ggatttattg agtatttttt catacagagt tcagtatact
     3661 ccaaaaactt tctgtttccc agttgtctga cagatatatt tattgaaaat ttaagattat
     3721 cgtatccaag cttgcttagc tcctgtacct ttgcaaaaac ttcttcaatt acccatccac
     3781 ctatactttc tataaaaccc gtttcttcgg caaccggaat aaacgttgcc gggaacaaaa
     3841 cgcctttagc aggatgcttc catcttataa gagcttcaaa gcagtacagg acattatttt
     3901 tagcatcaaa aacaggctga taacataaaa aaaattcttt ttctgaaagt gctttttcaa
     3961 aatctttttc tatttccagt ttttcaataa tgctttttgc catcttttcg tcgtaatagt
     4021 taaactttct gtttccttta atctgagatg ttcttacagc aacagatgct tttctgataa
     4081 gttcttcaag cgacgtacca tcttcaggat atgctacagc acctatgtac cctaaaattc
     4141 ttatctttac gccagatatt tcaaatgact cttcaactat attaagaagt ctctctgccg
     4201 ttttctgaat atatagcagt tccgtttttt cgagtataaa cacgaatgca tcatctttcc
     4261 atctgaaaac cttttcagta gagtttatgc tgcccattaa tttttccata gtttgcttta
     4321 gccatacatc tgccgcttca taaccgatgg tatcgtttac tactttaata tccgaaacgt
     4381 caagataaag caaagtaaag ggttttgaac aatgagttag ttcttctccg aatactttca
     4441 gataatttct gttaggcatg caattgtgta agctatcaaa ataagacaat ctagaattct
     4501 gttctttata gtcatgtgct aaacgttttt tttcagatag acaggtatct gtttcaaaaa
     4561 taaaattgaa ctgattgttc atctcattct gttctctttt acaaaaaaga ttgcaagaga
     4621 taaaatctct taagtactct aaaaacttta ctttttttac ggtatctttt tctatttcct
     4681 cgcttataat gcggatgaga taagcctgta ttttttttcc ggtacttatc ggaagaaggc
     4741 aaactctact tttttcaccg gggataaagc tatactccga aagtaaaaag ctttcctgaa
     4801 gagacatatt aatattatca attattattc cttttatgcc gtggctgaaa agtaactttc
     4861 ctgagcagtc cataataccc agtggaagtt ttgtcataat gcttagcagt tcgatacatt
     4921 cattaattct ttgataattc acataagaca taaaatcttc catgcttttt gcacctcttg
     4981 tgatgattat ttttagagaa aagattttgg acaagagaga tataaaaatt atttaatacc
     5041 tcattgttaa tggtgcaaac atatgactat tttaaatatg ctactataat taaacttcag
     5101 tattcttttt gatattttta agaatactgt tttttatttg gtaggaagca ttctgtctga
     5161 tcttcctaaa agataatcaa cactcacttc aaaaaaattt gcatatttta agagtatatc
     5221 caagctgggc atgctggaat atccgctctc aatacgccat atagtgttgn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnna tacatgaata ctggcgaaaa aataaaaaat
     5401 ttacgggaag aaaaaggttt atcaagagaa aagttcggag cacttataaa tatagcagga
     5461 aacagtgttt acaggataga aaacggattt aaccttcctg atgcaaaaac gcttcttaat
     5521 atttcaaaga cacttgatgt atcaattgac tatcttttgg ggttgtctga agtaaggacc
     5581 atacaaaaag aaaacgttga taaaataccg tatgatatca aacagaagct tttgaaactc
     5641 gaaagaatac ataaccaaaa tcttcctgaa aagcttgaga gaatcggtgt cgagcttata
     5701 gaaatttcaa agctactgaa agaaatctga ttgacacaga aaatattttt taactaatta
     5761 attttaattt ttcttggtca taatcaactt tatcatcttt ttgagctaaa aaaatagctt
     5821 ttttatttaa caaagctatg atttcgaatt tttattgatt tatttttgtg ctatttataa
     5881 tagtataaaa attaaaaagg cggtaaatat gtttagtgat aagttaaagg aactccgaca
     5941 gaaaaataat ttgactcagg aacaacttgc tcagaagatt aacaccacca gaaaaaacat
     6001 atctgcatat gaaacaggcc gttctgcacc tatgcacaga acttagctga tgattgcaga
     6061 tatttttaat gtatcaactg actatcttct tgaaagaagt atacctgaaa atattatgtt
     6121 gaatactttg acagggcaga atgttccgga tacggtactt caaaagctat taaagttgga
     6181 cagaatagaa acagaaaact tcccgatcgg ctggaaagat agcatatgat cttttggcaa
     6241 tttctaaaaa actcaaaagt atataaggtc ttctaaatgc tctttacaat aaataatacc
     6301 ctaaatcata gctccgtgat tagggtatta ttattcagtg cttactcata ccgtttttta
     6361 gtatttctat aaaactttta taatactcta ataacttttt cttatctttt accttataaa
     6421 acagttccag aatatcacta agattggtaa aaaagtattt gagggatctt tcaagtacct
     6481 ccatcggaat atcgtcgcgc agagtacctg actctgccgc tatatttatt attttgctga
     6541 aggttttatc tatttctggt ttaaaactat tcataacttt ctcatatata ctgtttcctt
     6601 tttcttttgc aaagcttacc gaaaaatcat aaaataatct atgattgaaa gcatattcga
     6661 ttacggtttc cgcttgcatc tggagcaaga gaaaaatatc gcattccagt ccattttctg
     6721 aaaaggattt ctccaaaagt tgttttttta tttcaagtgc ctttctcact aggcatatat
     6781 ataactcttc cttattttga aagtgatagt aaaaagttcc tttacttatt ccagtatttt
     6841 tgagaatact gttaagcgaa gcatttttat atcccttttc ggaaaattct tttaatgcag
     6901 tttcatagag aaggctttct ttttcaaatg aatcagtttc caaaaaaatc accccagtct
     6961 gagacctatt tttcttttaa gtttcgaaga aagaagtacc aacaaaagag tattatatac
     7021 taccgatatt acagaactta gaaaaatact gccatcaatc ccaaggtaaa gctgccatat
     7081 tatattgaat gtccagtaac ttggtattat gccaaagacc cattgtatag agcttggtaa
     7141 aagcaaagaa agcataggaa caaagacaag tatgaaagtt acaattttag tatacgcaat
     7201 ggccatattt tgggtatctg caagtgttcc tattataaaa cctaccgtaa gtgagagcag
     7261 cattccgctt aagataacaa ggagataatt ttttatgctg aactgaaatc ctgtcattat
     7321 tagctgagat aaaaaagata ctataataga gtaggaaaca gccactatga ctttggaagt
     7381 tatgtagtca gaaagcctta cgggagaaac tgccagtgca ttaatcattc cgctttcttt
     7441 atcgtctacc atggtaaaac cacatataac tcctgatata gatatgacaa agataagaat
     7501 cattgaaata ataagctctg atattcccga ttttttagta cctatatctg ttacaggtac
     7561 ttgtggactt cctttttcaa tgtcatgtat aacggcattg agaatgcgaa tgattcctgg
     7621 ttcttcatca ccctgaagaa caacttccaa gctgttatct ttaaaatcaa tgccaggtat
     7681 atcggtaaaa gccattatag agttttcaaa atttttacga tcggtagttt ttataacttc
     7741 aaaatatgaa gcaagctttt tttcatattc taccggaaga ttttgagaaa caaaaatctt
     7801 agttgttcca ctgcttatag aaggaatcat aagccttact gcaaaagcta ttactaaagg
     7861 aataagaaac ataaaaatca tcatcttatc attttttata tttgagatat ctttttttga
     7921 aagagcaaca attctcataa aatcaccttt tttattagag ttttccttac agaaaagtat
     7981 gttgccggaa aaaat
//
LOCUS       DAOSHR010000090         7956 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_7829_length_7956_cov_5.692191, whole genome shotgun sequence.
ACCESSION   DAOSHR010000090 DAOSHR010000000
VERSION     DAOSHR010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 7956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7956
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7829_length_7956_cov_5.692191"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(46..432)
                     /locus_tag="PLU28_06155"
     CDS             complement(46..432)
                     /locus_tag="PLU28_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34682.1"
                     /translation="MKRTVFILILIALLVLLGVWMFVLGRQRTIVMRNEDIDRQGITY
                     EAAQIVGISFDGAEEKEITYDDAAIFYLVGSSHKLKLRVADEDGNTLRVVEKTIKLDL
                     SKNISVDIPELLENKEEVIKLTGKMW"
     gene            complement(452..1582)
                     /locus_tag="PLU28_06160"
     CDS             complement(452..1582)
                     /locus_tag="PLU28_06160"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG34683.1"
                     /translation="MSQNLTFTEKLKNFFISNAVPILFVVITLLAVPVSGFSANYLIQ
                     EMITRIGRNSFLVLSLLLPIIAGMGLNFGMVLGAMAGQIGLIFITDWSIVGIPGLVLA
                     MLISTPISILLGILCGSVLNRAKGREMATSYILGFFINGVYQLVVLYFMGGIIPVKSP
                     SLVLSRGYGIRNTVALDGVRQVFDNMIPLKIGEISIPLGTFFIIALLCLFILWFRKTK
                     LGQDMRAIAQDMKVAQSAGIPVDRTRIISIVISTVLAGYGQIIFLQNMGTLSTYNSHD
                     QTGVFSIAAILIGGATVSRATIPNVFIGVILFHMMFVVSPMAGKNLIGQAMLGEYFRQ
                     FISYGIIAIALVLHAWRRQKDKEMERRSLRGVKAEKQPQAEK"
     gene            complement(1579..2622)
                     /locus_tag="PLU28_06165"
     CDS             complement(1579..2622)
                     /locus_tag="PLU28_06165"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34684.1"
                     /translation="MNRLRQFVNKAGWPRVIIGFFLLSLFVAAPFAEVSLAASIKDTL
                     VRFGMNGVLVLSMVPMVQSGCGLNFGLPLGVIAGLLGAVTTMEMGITGIMGFITAVIL
                     SCAIAAVLGWGYGKMLNRVKGEEMVISTYVGLSSVMFMCMMWLVLPFKSPAMVWGYGG
                     SGLRTTITLEGYWFNIIDNFLAIKIGDFTFPTGLFIFFAVMAFLVWAFFHTKTGTAMT
                     AVGSNPDFARASGIDVNKMRTISVIISTVFGAVGILVYQQSYGFIQLYTSPLFMAFPA
                     VAAILLGGASVHKATITNVIVGTILFQGILTMTPSVINSFLKTDLSEVIRIIVSNGMI
                     VYALTRKTTKVKS"
     gene            complement(2619..4232)
                     /locus_tag="PLU28_06170"
     CDS             complement(2619..4232)
                     /locus_tag="PLU28_06170"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOG34685.1"
                     /translation="MVSEQVVLKMENITKDYYGNRVLENVSFELKKGEILGLIGENGA
                     GKSTLMNILFGMSVISETGGYQGKVIIDGKEVNFKNAFDALDAGIGMVHQEFSLIPGF
                     TTIENIMLNRESLKYNFLVEVFGSRLKTLDRTDMKDRAEKAISALGVKIDPNMLIAEM
                     PVGHKQFTEIARELDRKNMKILVLDEPTAVLTESEADILIQSLKRLSDLGIAIIFISH
                     RLKEIIDVCDKILVLRDGKTVKESPAKETSIRQIAQWMVGRNIEEKKDEVKNEKKLGD
                     TVLKVENLWVDMPGETVRDVSFEVREGEIFGIGGLAGQGKLGIPNGIMGLYEAGGRIE
                     VFGKSLEMNVPVKALKAGMAFVAEDRRGVGLLLEEPIDWNISFTVMQAQNKYLKSFIG
                     GLVKWRDDKKISELAKEYIKILDIKCVDEKQPVQELSGGNQQKICLAKAFAVNPKILF
                     VSEPTRGIDIGAKKLVLDTLKQYNQKYGTTIIMTSSELEELRSICDNIAIVDEGKVAG
                     ILPATAATEDFGLLMAGKVDESKEKVSAQ"
     gene            complement(4302..5480)
                     /locus_tag="PLU28_06175"
     CDS             complement(4302..5480)
                     /locus_tag="PLU28_06175"
                     /inference="COORDINATES: protein motif:HMM:NF024095.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HOG34686.1"
                     /translation="MKKLLVLLVVAMFSVFAFSAAAFHIGVVTGTVSQSEDDLRGAEA
                     LIKNYGDSASGGMITHLTYPDNFMSEMETTIAQIAGLADDPLMKAIIVNQAVPGTAEA
                     FRKVREKRPDILLFAGEPHEDPGVIAPVSDIAINADNIARGYTIIYAAKMLGAKTFVH
                     ISFPRHMGYELLSRRRNIMEQACKDLGLKFVFETAPDPTSDVGVAGAQQFVLEKVPAW
                     LKKYGQETAFFCTNDAHTDPLLKRIAELGGYFIEADLPSPLMGYPGALGVDLKAEQGN
                     WPAILNKVEEAVVAKGGKGRMGTWAYSYGYATTAGLGEYAKNIIEGKAKLNNLKDLLA
                     AYNVYTPGAEWNGSNYKDMNTGIKSKNHFLLYQDTYVFGKGYLGMTKITVPEKYFGIK
                     "
     gene            5785..5919
                     /locus_tag="PLU28_06180"
     CDS             5785..5919
                     /locus_tag="PLU28_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34687.1"
                     /translation="MNKEVENYLQKIGLTDDDIKRLVKSEKQKTASMCYAVNEYDEYL
                     "
     gene            6047..7834
                     /locus_tag="PLU28_06185"
     CDS             6047..7834
                     /locus_tag="PLU28_06185"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOG34688.1"
                     /translation="MDEKEKEVPKGENPSEEVVRLKAKILEYERTMEYQKKLEDSLKK
                     SEEIYKLLTENSTDIVSKHSSDGIFTFVSPSIKNILGYSAEELIGTSIYQLFNPQDLI
                     SVKRAFSSILALPTSSTFSYRIKKKDGAYLWFETTGKSIRDEFSGVVKGIILISRNIN
                     EKKLIESELTFRNDLRNLTEKISNRLNSSKIQDIEDDLIALSTDVAKLFSYDDGDVFI
                     FNEEFKSIMPNVYGKLRANIVLEHTQPDLLDDFQKYTGKSIRSFAFFPLSIEKNVIGM
                     LALYNFQTESSITESLNNNIRTVLEIFASALGRMLDNYKIVSLNSTIKVILDNIPQAY
                     IHINNEGLISDINSKAQEYLPLKKDDPFAKIVSDNSIFLKDSKFDREFKIAWEKRLPV
                     SFEEFDISDNSWYEVRMIPASDGLSIFFRDITQQRTSEEKLVEYATKDSLTGVLNRRA
                     GLIHLQKLINICKTQNKNLCIGYIDIDNLKKINDKYGQNEGDFLLTLAIKTLRENLRE
                     GDIICRVGGDEFLIIFPDTSIEEILQLWQEIDSIFRQFNETKKKPYSIYINRGFSQFD
                     QQKNSTLDAMLANADRELTAFSKKPKSSK"
BASE COUNT         2413 a         1776 c         1368 g         2399 t
ORIGIN      
        1 taaaaaaatc cagagcctga tcattactca ggctctggaa taatttcacc acatctttcc
       61 ggtaagcttt atcacttctt ccttgttttc caaaagctcc ggaatatcaa cacttatgtt
      121 ctttgaaaga tcaagcttta tagttttttc aacaacacgc aaagtatttc cgtcctcatc
      181 ggcaactctt agttttaact tatgtgaaga gcctaccaga taaaatatgg cagcatcgtc
      241 atatgtgatt tctttttcct cagctccgtc aaaagaaatt cctacaatct gtgcggcttc
      301 atatgtgata ccctgtctgt caatatcttc atttctcata acaattgtac gctgtcttcc
      361 aaggacaaac atccaaactc ctaacaatac aagaagtgct atcagaataa gtataaaaac
      421 tgttcttttc acaataatag cctcctctgc cttatttctc agcctgaggc tgtttttctg
      481 ctttcacgcc tctaagactt cttctttcca tttctttatc tttctgacgc ctccatgcat
      541 gaagaaccag cgcaatagct attattccat aagaaataaa ctgtctgaaa tattcaccca
      601 gcattgcctg tccgatgaga ttctttcctg ccataggaga aacaacaaac atcatgtgaa
      661 aaagtattac tcctatgaac acattcggaa tggttgctct ggaaaccgtt gcaccgccta
      721 taagtatggc agctattgaa aatactccgg tctggtcatg gctgttgtaa gtgctcaatg
      781 tacccatgtt ctgaagaaag attatctgtc cgtatccggc cagaaccgtt gaaattacta
      841 tcgaaatgat tcttgtacgg tcaaccggta taccggcgga ctgtgcaact ttcatatcct
      901 gggctatggc tctcatatcc tgtccaagct tggtctttct gaaccacagt atgaataagc
      961 atagcaaagc tatgatgaaa aaagtgccca gaggtatgga aatctctcct atctttaatg
     1021 gaatcatatt gtcaaatacc tgtctgacac cgtcaagagc gacggtgttt cttatgccgt
     1081 atcctcttga aagaaccaga gaagggcttt ttacaggaat tatccctccc atgaaataaa
     1141 gaacaacaag ctgataaaca ccattaatga aaaaacccag tatgtacgac gttgccattt
     1201 ctctcccctt agccctgttg aggacacttc cgcaaagaat ccccagaagt atggaaatag
     1261 gagttgatat gagcatggca agtacaagtc caggtatgcc tacgatagac cagtctgtga
     1321 taaatatgag tcctatctga cctgccattg ctcccagcac cataccgaaa ttcagaccca
     1381 tacccgctat aataggcaac aacagggata gaaccagaaa tgagtttctg ccaattctgg
     1441 ttatcatctc ctgtatcaga tagttggcac tgaacccgga aacgggaaca gcaagcaatg
     1501 taataactac aaaaagtatc ggaacagcgt tgcttataaa aaaattcttt aatttttctg
     1561 taaaagtaag attctgactc atgatttcac cttcgttgtc tttctggtaa gtgcataaac
     1621 aatcataccg ttggaaacta ttattcttat aacttccgaa agatctgtct ttaagaaact
     1681 gtttattacg gaaggagtca ttgtaagtat accctgaaag agtattgttc ctacgataac
     1741 gttggttata gtagccttat gaaccgatgc ccctcccaga aggatggcag ccacagccgg
     1801 aaaagccatg aaaagtggac ttgtatacaa ctgtataaac ccatagctct gctgataaac
     1861 caaaatgccg actgcaccga atactgtaga aattatcacc gagatagttc tcattttgtt
     1921 tacatctatt cctgaagccc ttgcaaaatc ggggttagaa ccgacagccg tcatggcagt
     1981 acctgttttg gtatgaaaaa atgcccatac caaaaatgcc attactgcaa aaaatataaa
     2041 caaacccgta ggaaacgtaa aatcgcctat ttttatagcc aaaaaattat ctataatatt
     2101 gaaccagtat ccttcaaggg taatggttgt tctcagaccc gatcccccgt acccccatac
     2161 catcgcagga ctcttgaatg gaaggacaag ccacatcata cacataaaca ttacggaaga
     2221 aaggccaacg tacgttgata tgaccatctc ttcgcccttt accctgttaa gcatctttcc
     2281 gtatccccag cccagtacag ccgcaattgc gcatgaaaga ataaccgccg taataaagcc
     2341 cataatgccg gttataccca tttccatagt ggttacagca cccaaaagcc cagctattac
     2401 tcccaaagga agaccaaaat taagtccgca tcccgactga accatcggca ccatggaaag
     2461 cacaagcaca ccgttcatac caaatcttac cagcgtatct tttattgatg cggcaagact
     2521 gacctctgca aaaggagcag ccacaaaaag ggaaagaaga aaaaaaccga ttataaccct
     2581 tggccatcca gccttattaa caaactgtct taatctattc attgtgcgct caccttctct
     2641 ttgctttcat caacctttcc tgccataagc agaccaaagt cttcggttgc tgccgttgca
     2701 ggaagtatac ctgccacttt accttcgtcc actatggcta tgttgtcaca tatagacctt
     2761 agctcttcca actcggagga ggtcattatt atagtcgtac cgtacttttg attatactgc
     2821 ttaagagtat ccagaaccag tttttttgca cctatatcga ttcctcttgt cggctctgaa
     2881 acgaacagaa tcttcggatt cacggcaaat gctttggcaa gacatatctt ctgctgattt
     2941 ccaccagaaa gctcctgaac aggctgtttt tcatccacac atttgatatc cagaatcttt
     3001 atgtactctt ttgccagctc cgaaatcttt ttatcgtctc tccactttac cagtccgcct
     3061 atgaagctct taagatattt gttttgggcc tgcattacag taaaggatat attccagtct
     3121 attggctctt caagcaacag acccacacct ctcctgtctt ctgctacaaa agccatacct
     3181 gctttaaggg cttttaccgg tacgttcatc tcaaggcttt ttccaaaaac ctcaatcctt
     3241 ccgccggctt cataaagtcc cattattccg tttggaatac ccagcttgcc ctggcctgcc
     3301 aaccctccga ttccgaatat ctcaccttca cgaacttcaa agcttacatc cctgacagtc
     3361 tctcctggca tgtcaaccca aagattttca accttcaaaa ccgtatctcc aagttttttt
     3421 tcatttttta cttcgtcttt tttttcttct atgtttcttc cgaccatcca ctgggctatc
     3481 tgccttatcg aggtttcttt cgcaggactc tccttgacag tcttgccatc tcttagaacc
     3541 agaatcttat cacatacatc tattatttct ttgagcctgt gcgaaataaa aattatggct
     3601 attcccagat cagaaagcct tttcagagac tgtatcaaaa tgtctgcttc actttctgtg
     3661 agaactgccg taggctcgtc caaaaccaga atcttcatat tttttctgtc aagctctctg
     3721 gctatctcgg taaactgctt atgaccgacc ggcatctccg ctatcagcat attcggatct
     3781 atttttactc ccagtgcact tattgccttt tccgctctat ctttcatatc cgtacggtca
     3841 agagtcttaa gcctggatcc aaatacctct acaagaaagt tgtatttaag ggactctctg
     3901 ttcagcatta tattttcaat ggtggtaaac cccggaatta gcgagaactc ctgatgaacc
     3961 atgcctattc cggcatccag agcatcaaaa gcatttttaa aattcacttc ctttccgtct
     4021 attatgacct tgccctggta accccctgtt tcagatatca ccgacatacc gaaaaggata
     4081 ttcattaaag tagatttccc ggcaccgttt tctcctatca gacccagaat ttcacctttt
     4141 ttcagttcaa agcttacgtt ttccaaaacc ctgtttccat aataatcctt agttatgttc
     4201 tccattttca gaacaacctg ttcagatacc atcaactcca cctctttatc aaaaagataa
     4261 gggaacaaaa gttcccttat ctcatttcgg aaagttttaa cttactttat accgaagtac
     4321 ttttcaggaa cagttatctt tgtcattccc agataacctt ttccgaaaac ataagtatcc
     4381 tggtaaagca ggaagtgatt cttagacttt atgcctgtat tcatatcctt atagttactt
     4441 ccgttccatt ctgcgccggg tgtataaaca ttatatgcgg cgagaagatc cttaaggttg
     4501 ttcagcttgg ctttgccctc aattatattc tttgcgtact caccaagacc tgctgtagtt
     4561 gcataaccat atgagtaagc ccatgttccc attctgcctt ttccaccttt ggctacgact
     4621 gcctcttcta ccttattaag tattgcaggc cagtttccct gttctgcttt aaggtcaact
     4681 ccaagtgcac caggatatcc catgagagga gacggaagat cggcttctat aaagtatccg
     4741 ccaagctctg ctattctctt gagaagagga tctgtatgtg catcgtttgt acagaaaaat
     4801 gctgtttcct gaccatactt cttaagccat gcgggaacct tttcaagaac aaactgctgt
     4861 gctcctgcaa ctccaacatc gcttgtagga tccggggctg tttcaaatac aaactttaac
     4921 cctagatctt tacatgcctg ttccattatg tttctccttc ttgaaagaag ctcataaccc
     4981 atatgtcttg ggaaggatat atgtacgaat gtttttgctc ctaacatctt tgcagcatag
     5041 attatggtat atcctctggc aatgttgtct gcgtttatcg ctatgtctga aactggtgca
     5101 ataacgcctg ggtcttcatg aggctctcct gcaaaaagaa gaatgtcagg acgtttttct
     5161 ctaacctttc tgaaagcttc agcagttccc ggaacagcct gattaacgat tattgctttc
     5221 atcaaaggat catctgcaag acctgcaatt tgagcaatgg ttgtttccat ctcagacata
     5281 aagttgtccg gatatgtaag gtgggtaatc ataccgccgg atgccgagtc tccgtaattc
     5341 tttataagag cctctgctcc tcttagatca tcttcagact gggaaactgt accggttact
     5401 actccaatgt gaaaagctgc tgctgagaat gcgaaaaccg aaaacatcgc tactaccagt
     5461 aaaaccaata gctttttcat tcatcttccc ccctatggtt tttattagcg tttgtaataa
     5521 tacgcaataa aatgataaca tattgttctt tagttgtcaa atttgaaaaa cttatcatta
     5581 atattatctg agaatattaa tactatatat tttaatgaaa tttttgatgg gtttcttgta
     5641 taaagtatta atgtcatgtg tttccggtta atcttcaaaa gctactccaa ggtaaccgaa
     5701 agtaaattca tgctttcaga aagaataatc tttcattatg atataatata taatcatctg
     5761 tgaaaatact ttcccggagg aaatatgaat aaagaagttg aaaattatct tcagaaaata
     5821 ggacttaccg atgatgatat aaagcggctt gtcaaatcag aaaaacagaa aacagccagt
     5881 atgtgttatg ctgtgaatga gtatgatgag tatctttaga taagtatttt ggatattacc
     5941 tacgacatta accttaaggt caagatctct ctaattaaga atcatgaaaa ataagatttt
     6001 atgttgtata atacatacta cattaatata aaacggaggt tgatctatgg acgaaaaaga
     6061 aaaggaagtt cccaaagggg agaatccctc tgaggaagta gtaagactca aggcaaagat
     6121 tcttgaatac gaaagaacta tggagtatca gaagaaactt gaagactctc tcaaaaagag
     6181 cgaagagatc tataagcttc tcacagaaaa ctccacggat atagtctcca aacattcttc
     6241 cgacggaatc ttcacctttg tttctccatc cataaaaaat atactgggat attcggcgga
     6301 ggagcttata ggtacatcta tctatcaact ttttaatcct caggatctta taagcgtgaa
     6361 aagagctttt tcctctattc tggccttgcc gacatcatct actttttctt acagaataaa
     6421 gaaaaaagac ggtgcgtacc tttggtttga aactacaggc aagtccatca gagatgaatt
     6481 ttccggagtt gtaaagggta ttatccttat ttcacgaaat attaacgaaa aaaagcttat
     6541 agagagcgaa cttactttca gaaacgacct tcgcaacctc accgaaaaga tatcaaatag
     6601 gctaaattca tcaaaaattc aggatattga agatgatctc atagctcttt ccacagatgt
     6661 ggctaagctt ttttcatacg acgacggtga tgtgtttata ttcaatgaag agttcaagag
     6721 tataatgcct aacgtttacg gaaaacttag agcaaatata gtgcttgaac atactcagcc
     6781 tgatcttctt gatgattttc aaaagtacac cggaaaaagt atccgttctt ttgctttttt
     6841 tcctttaagt atagagaaaa acgtaatagg tatgcttgcg ctttacaatt tccagacgga
     6901 aagcagtatt acagagagtc ttaacaacaa tataagaact gttcttgaga tctttgcaag
     6961 tgcccttgga agaatgcttg acaactataa gattgtttct ctaaactcaa ccattaaggt
     7021 tattctggat aacataccgc aagcttacat acatataaac aacgaaggtc ttatatctga
     7081 tataaactct aaggcacagg agtatctgcc ccttaagaag gacgatcctt ttgccaaaat
     7141 tgtttccgac aactctatat ttttaaagga ttccaagttt gacagagagt ttaaaattgc
     7201 gtgggaaaaa agacttcctg tttcctttga agagttcgac atatccgaca actcatggta
     7261 tgaagtacgt atgatacctg cttcagacgg actcagtata ttcttcaggg atatcactca
     7321 acagagaact tctgaagaaa aacttgttga atatgcaaca aaagactcac ttaccggtgt
     7381 cctgaacaga agggcaggac tcatacacct gcaaaaacta ataaatatat gcaagactca
     7441 gaacaaaaac ctctgcatcg gttatataga cattgacaat ctgaaaaaga ttaacgataa
     7501 gtatggtcag aatgaaggtg attttcttct gacccttgcg attaaaactt tgcgcgaaaa
     7561 tctcagggaa ggagatataa tatgcagagt cggaggcgat gagtttctga taatatttcc
     7621 ggatacttca attgaagaaa tcttgcagct ctggcaagaa atagacagta tcttcaggca
     7681 gttcaacgag actaagaaga aaccttacag catttatata aacaggggtt tttctcagtt
     7741 cgatcagcag aaaaattcaa ctcttgatgc tatgcttgca aatgcagaca gggaacttac
     7801 agcattttca aagaaaccaa aaagttcaaa atagcttttt attatattaa atttgaagta
     7861 atccggcata ctcttaatgc cggattattt tttgccctta tttttttcag tttacactgg
     7921 atacttatta ttttaacctt caataacaga catata
//
LOCUS       DAOSHR010000091         7880 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_7932_length_7880_cov_5.407284, whole genome shotgun sequence.
ACCESSION   DAOSHR010000091 DAOSHR010000000
VERSION     DAOSHR010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 7880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7880
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7932_length_7880_cov_5.407284"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="PLU28_06190"
     CDS             <1..528
                     /locus_tag="PLU28_06190"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208166.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     pyruvate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HOG34689.1"
                     /translation="IVGFVEEFEFVKKIVVETADALIKESGIELEYKVGTMIEVPRAA
                     VVADNIGKSADFFSFGTNDLTQLTLGFSRDDYGKFVKDYIAKGIFESDPFQHIDRKGV
                     GKMMKMAMDLGRDANPKLKTGICGEHGGDPESIEFAHLLGLDYVSCSPYRVPIARLAA
                     AQAAVKYVRRKRVND"
     gene            775..1926
                     /locus_tag="PLU28_06195"
     CDS             775..1926
                     /locus_tag="PLU28_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOG34690.1"
                     /translation="MLQSIANFFVTSSGFASLTFGNIIMLIIGAVLIYVGISRDAEPL
                     LLIPIGFGIIMGNIPPIATGLLAAPVGDSLGGFLWYINRGMYLGIYPPLIFLGIGALT
                     DFSYLIANPILMLLGAAAQVGIFITFIIANFLGFDLFQAASIGIIGGADGPTAIFLSS
                     KFAPELVAIIAVAGYSYIALIPILQPIVSRFLTTKKERLIKMKKVRTVSKKERMIFPV
                     ISTIIVALFVPQSLNLVGMLMLGNLLKESGLTERLAEASARFILDSVTILLMISVGSA
                     ATAELFLTPQSLKIVFLGAFAFIVSLSSGIFFAKFMNLFLKEKLNPLIGAAGVSAVPD
                     SARVAQTIAQKDDPTNFILMHAMAPNVAGVIGSAVAAGVFLNIIPKLMP"
     gene            1940..2170
                     /locus_tag="PLU28_06200"
     CDS             1940..2170
                     /locus_tag="PLU28_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOG34691.1"
                     /translation="MASKKAYNVEINRSYCKSCGLCYWICPTKTIIKGKYDKPEIPDQ
                     TKCIGCLQCERICPDFAINVVEIVEEKSEENK"
     gene            2188..3345
                     /locus_tag="PLU28_06205"
     CDS             2188..3345
                     /locus_tag="PLU28_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HOG34692.1"
                     /translation="MSRMVIMQGNEACALAALKAGCRFYAGYPITPSSEVAETMAREL
                     PKVGGTFIQMEDEISSAAAIIGASLSGVKSMTATSGPGFSLMQEGIGYGVMTETPCVF
                     VNVMRGGPSTGMPTKPAQGDIMQLRWGTHGDHQIIALYPSTVEEVYKYTIKAFNYAEK
                     YRTPVFLVMDETLGHMRESFFLTDEEENPEIIQRIKDTEFSDDELFHPFDLGEDRTVA
                     PLVGMGKTRFHVSGLVHDETGFPAGQAEVAEKMIKHLDSKIRMYSEEISIYEEYMLSD
                     AEIVVVAFGTTARSALRAVKMARSDKIPVGLFKPVTIWPAPVSRIKKLFRSANAFIIP
                     EMNMGQYAQELSKLNKTNKHIEMLNKINGELITPEEILDSITKVWIQLTEI"
     gene            3478..3762
                     /locus_tag="PLU28_06210"
     CDS             3478..3762
                     /locus_tag="PLU28_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HOG34693.1"
                     /translation="MEVLKVSHSSAPNKVAGAIAGVLSKNEEVEIQAIGAGAVNQAVK
                     AIAIARRFVETDGKLVYVIPGFVEVEVGNEKRTGIKFKVAVSMNEGEEKH"
     gene            3762..4010
                     /locus_tag="PLU28_06215"
     CDS             3762..4010
                     /locus_tag="PLU28_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34694.1"
                     /translation="MKIDEKIINEIMTACLKDTRLFSIVIDISQKSEEERYLIRKKAS
                     IVLNEVNGVEKEALKFYYILTEDGVAQEIIRRMNDAVK"
     gene            3994..4911
                     /locus_tag="PLU28_06220"
     CDS             3994..4911
                     /locus_tag="PLU28_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HOG34695.1"
                     /translation="MMPSNNKGELPRTVGGQAVIEGVMMKAVNTVVAVKKPDGSIAIK
                     RMPDKSWGVIEKIPLIRGFFVLIHSMIVGMNALSYSAKVSGDEKEEFTTKDMVFAFLF
                     AILIAVLGFGVLPVIITKPLNIKSEFWFAFVEGIIRAVFVIVYIWGISHLKDIKRVFQ
                     YHGAEHKSVYTYEKKEELTSANAKKYTTLHPRCGTSFLIITVFASIVVFAISGALGMN
                     SMWEKVIARIILLPLVAGVAYEFQRLTAKIIDNPLGKILSYPGMSLQKITTVEPDEEQ
                     LKIGLLSLKYALKEDFEGEVTVSLSEEVK"
     gene            5150..>5424
                     /locus_tag="PLU28_06225"
     CDS             5150..>5424
                     /locus_tag="PLU28_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34696.1"
                     /translation="MNIGIMGLGTYTPKTFISAKEISEQTGIPEDVISVKFGVKQKPI
                     PGPEDTTSHMGIEAARAAIANAGISAQEIDLIIWNGAQHKDYPCWLAG"
     assembly_gap    5425..5524
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5525..6318
                     /locus_tag="PLU28_06230"
     CDS             <5525..6318
                     /locus_tag="PLU28_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="HOG34697.1"
                     /translation="LIIWNGAQHKDYPCWLAGLKVAEVLGAKNAWSFDMEAMCGSMMS
                     GMEVAKSMMTANEDIKTVLLVSGYRNLDLISYKVPETSFMFDIGAGGAAMVLRKGYDR
                     NVILGTAFKGDGSFSEDCVVEKGGSKSWPMAEKDFENMHFVVRDPATFKARLGEKTMP
                     NFYWVIRQALKKSGNLTDKDINYLAILHFKRSAHMEVLKELGLKEDQTTYLDDYGHIG
                     QNDQVLSIELGLKSGKIKDGDNIVMVGAGLGFVWAGAVVRWGKAL"
     gene            6343..7080
                     /gene="fabG"
                     /locus_tag="PLU28_06235"
     CDS             6343..7080
                     /gene="fabG"
                     /locus_tag="PLU28_06235"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HOG34698.1"
                     /translation="MRMNGKVCVVTGGARGIGKEIVTKFANEGALVVFACDMNKEALD
                     ELEKEFKNVKGYILNVTDREGIKVFVEKVRAEYGRIDALVNNAGITRDALIQKMSEED
                     WDAVINVNLKGVFNMTQAIGPLMMETGSGSIVSISSVVGLDGNVGQTNYAATKGGVVA
                     MTKTWSKEFARKGAKVRANAVAPGFVRTPMTDKVPEKILEDMKNKALLKRLGEAEDIA
                     NAVLFLSSEEASFVTGQVLRVDGGLVI"
     gene            7099..>7880
                     /locus_tag="PLU28_06240"
     CDS             7099..>7880
                     /locus_tag="PLU28_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyrate--acetyl-CoA CoA transferase"
                     /protein_id="HOG34699.1"
                     /translation="MSSWTSVYNNKKRSVEEAILSLPKRCTVVVGLAAMEAQGLLSNL
                     HKFRDNFEYIKVLTCLNMKDYPFISSKDSEGILENHTWFMSVPTRNARDSGLKTVDYI
                     PNNLHMSSTDKFVSEKEDGNKIVFWGTVTPMNEVSGFMSLGLSNVYEMDILEKADYTV
                     LEVNDKVPWTHGETQVHIAQADVVTESSWAIPDLSVVEPAQTEIQIAQYIADLVRDGS
                     TVQIGIGGIPNAVAKLLKNKKDLGVHTEMFTESMIELFENGI"
BASE COUNT         2422 a         1266 c         1846 g         2246 t
ORIGIN      
        1 atagttggtt ttgttgaaga gtttgaattt gttaagaaaa ttgttgttga aactgccgat
       61 gctcttatta aagaatcagg aatagagctt gagtataaag tcggtactat gatagaagtt
      121 ccaagagcgg cagtagtagc cgataacata ggaaaatcag ctgatttctt cagctttggg
      181 acaaacgacc tgacccagct cactcttggc ttctcaagag atgattatgg taagttcgtc
      241 aaagattata tagccaaagg aatatttgaa tctgatcctt tccagcatat tgacagaaaa
      301 ggtgtaggca agatgatgaa gatggctatg gatcttggca gagatgctaa tcccaagctt
      361 aagacaggaa tctgcgggga acacggtgga gatcctgaat caatagagtt cgcacatctg
      421 ctaggtttgg attatgtaag ctgttcacca tacagagtac ctatagcaag gcttgcagca
      481 gctcaggcag cagtaaagta tgtaagaagg aaaagagtta atgactaaat ttgagtaatg
      541 atcaaagcgt gtctggcaca atgcccggca cgctttttct atggttttaa agattatttt
      601 caaagactct aaaaaattac cttacgattc aaacagacgg caatagtaac catgtgtaat
      661 tgttatatag ttttttgtta actttgctaa ggttgaaaaa acatccttca gaggttataa
      721 taataagtgt agatacatct taaagcataa gttcatattg ggaggttttt aaaaatgttg
      781 cagagtattg ccaacttttt tgtaacaagc agcggtttcg cttccttgac atttggcaac
      841 atcataatgc tcattattgg agcggttctg atatatgtcg gcatatccag ggatgccgag
      901 cctttgctct taattccgat aggtttcgga ataataatgg gtaatatccc ccctatagcc
      961 acaggacttc tggcagcacc tgtaggagat agtcttggag ggtttctatg gtatataaac
     1021 aggggaatgt atcttggaat atatcctcca ttgatatttc ttggtatagg tgctttgaca
     1081 gacttttcat atctgattgc caatcccatt ctgatgcttt tgggagcagc ggcacaggtc
     1141 ggaatattca taacctttat tatcgccaac tttcttgggt ttgacctttt ccaggcggct
     1201 tctataggaa taataggcgg tgccgacggg ccgactgcaa tttttctttc ttcgaagttt
     1261 gcacctgagc ttgtggcaat aattgctgtg gccggttact cgtatattgc tcttataccg
     1321 atactgcagc cgattgtatc caggttttta accacaaaaa aagaaagact tatcaagatg
     1381 aagaaggtaa ggactgtttc caaaaaagaa agaatgatct tccctgttat ttctacaata
     1441 atagttgcgt tgtttgttcc tcagtcactt aacctggtcg gtatgcttat gcttggaaac
     1501 cttcttaagg aaagcggact tactgaaaga cttgccgaag cttcggcgag atttatcctt
     1561 gacagtgtta cgattcttct tatgatatct gtcgggtcag ctgcaactgc agagcttttc
     1621 cttacgcctc agtcgctcaa aatagtattt cttggtgcct ttgcttttat agtatcgttg
     1681 tcatccggaa tattctttgc caagttcatg aatttgttcc taaaagagaa gcttaacccg
     1741 cttatcgggg cagcaggtgt ttcggccgtt ccggattctg caagggttgc tcagactata
     1801 gctcagaagg atgacccaac caacttcata cttatgcacg caatggctcc taatgttgct
     1861 ggagtaatag gttcagctgt ggcagcagga gtgtttttaa atataatacc aaaattaatg
     1921 ccttaacgga ggtgctgaaa tggcttcaaa aaaagcctat aatgttgaaa taaacaggag
     1981 ttactgcaaa agttgcggtt tatgttactg gatatgtccc actaagacta taatcaaagg
     2041 aaagtacgat aaacccgaga ttcctgatca gaccaaatgt ataggttgtc tccagtgtga
     2101 aaggatatgt ccggactttg ccatcaacgt tgtagaaatt gttgaggaaa agtcagaaga
     2161 aaacaaataa ttaggatggt ggataaaatg agcagaatgg ttattatgca aggaaacgaa
     2221 gcatgtgcat tggctgcgct aaaggccgga tgcaggtttt atgcaggata ccccataact
     2281 ccatcttctg aggtagctga gacgatggca cgcgagcttc cgaaagtagg aggtaccttc
     2341 attcagatgg aagatgaaat ttcaagtgct gcagcaataa taggagcttc actatcggga
     2401 gtaaaatcaa tgacggcaac aagcggtccc ggtttttctc ttatgcagga aggaatagga
     2461 tacggcgtta tgacagaaac cccgtgtgtt tttgtgaacg ttatgagagg cggtcccagc
     2521 acaggtatgc caaccaaacc tgcacagggc gatataatgc agctcagatg ggggacacat
     2581 ggagatcacc agataatcgc cctttatcct tcgacggttg aagaggtcta taagtacact
     2641 ataaaggctt tcaactatgc tgaaaagtac agaacacccg tatttcttgt catggatgaa
     2701 acacttggcc acatgaggga aagctttttc cttacagatg aagaagagaa tcctgagata
     2761 atacagagga ttaaagatac agagttttca gatgacgagc ttttccatcc gtttgatttg
     2821 ggcgaggaca ggacagtcgc gcctcttgtc gggatgggta agacaagatt tcatgtttca
     2881 ggtttggttc atgacgagac cggttttccg gcaggacagg ctgaagttgc cgaaaaaatg
     2941 attaagcacc ttgattcaaa aataagaatg tattctgaag aaatttctat atatgaagag
     3001 tatatgctca gtgatgccga aattgttgtg gttgctttcg gtactaccgc aagaagtgcc
     3061 ctcagagctg tgaagatggc cagaagcgat aagatacctg tcggtctttt taagcccgtt
     3121 actatttggc ctgctcctgt ttcgagaatt aaaaagcttt tccgttccgc aaatgcattt
     3181 attattcctg aaatgaacat gggacagtat gcccaggagt tatctaagct caacaagact
     3241 aataagcata ttgaaatgct caataagata aacggagagc ttataactcc ggaggagatt
     3301 ctggactcca taaccaaggt atggattcag ctcacagaaa tataaaattc attaaagtta
     3361 agccaaacgt aaaatgtttg gctttttatt tttaaagtgt ttaaaatact ttgtattttt
     3421 tttaaaatgt ggtaaaataa taataaagat ataaagtata aggaggagag ctatagaatg
     3481 gaagtattaa aagttagtca ttcatctgcc ccaaataaag tcgcaggagc tatagcggga
     3541 gttctttcaa aaaacgaaga ggttgagatt caggcaatag gagccggtgc agtcaatcag
     3601 gcagtaaaag caattgccat tgcgcggcgt tttgttgaaa ccgatggtaa gctggtttat
     3661 gtgatacccg gttttgttga agtcgaagtt ggaaatgaaa agcgtaccgg tattaagttt
     3721 aaagttgctg taagcatgaa tgagggtgaa gaaaagcatt aatgaagatt gatgagaaga
     3781 taattaatga gattatgacc gcgtgcttga aagacacgcg gcttttttcg atagtgattg
     3841 atatttcaca aaaaagtgaa gaagaaagat atcttataag aaaaaaagct tcgattgtat
     3901 tgaatgaagt taatggagtt gaaaaagaag ctttaaaatt ttattatata cttacggaag
     3961 atggagtagc ccaagagatt ataaggagga tgaatgatgc cgtcaaataa taaaggggaa
     4021 ttaccgcgca ccgttggcgg gcaggcagtt attgaaggag taatgatgaa agcggtaaat
     4081 actgtcgtag ctgtaaaaaa gccagacgga agcatagcta taaaacgtat gcctgacaaa
     4141 tcatggggag taatagagaa aatccctctt ataagaggtt tttttgttct tattcactca
     4201 atgatagttg gcatgaatgc attatcttac tctgcaaagg tatctgggga tgagaaagag
     4261 gagtttacca ccaaggatat ggtttttgct tttctttttg ccatccttat agcagtcctc
     4321 ggttttggtg ttctgccggt tattataacc aaacctttga atataaaatc agagttttgg
     4381 ttcgcttttg tagagggcat aataagagca gtatttgtga ttgtttatat atggggtatt
     4441 tcgcatctta aagatattaa aagggttttt cagtatcatg gtgccgaaca caaaagtgtt
     4501 tatacatatg aaaaaaagga agagcttacc agtgccaatg ccaaaaagta cactacgctt
     4561 catcccagat gtggtacaag ctttcttata attacagttt ttgcatctat cgttgttttt
     4621 gccatttccg gagcgttggg aatgaacagt atgtgggaaa aagtaattgc acgtataatc
     4681 cttcttccac ttgtagcggg tgtggcatat gaatttcaga ggcttactgc caagataatt
     4741 gataatcctt tgggaaaaat tctttcttat ccgggaatgt cgcttcaaaa gatcactact
     4801 gtcgagccgg atgaagaaca gctcaagata ggactgttat ctttgaaata tgctttgaaa
     4861 gaggattttg aaggtgaggt tactgttagt ctttcagaag aagttaaata gttatggaat
     4921 ctgatcgggc ggcttttaag ccgccttttt tattttatac ccgtacattt tgttaatcta
     4981 caaaaaagac aggtatttaa aattatttac cgtaatttgg ttaaaactct tgataaacat
     5041 gtctagtcat attttatttt ttttgacttt tgttttaaaa tgagaagtga ttcacacaat
     5101 gagaagtgat tcatataaaa aacaacagta ttaaaaagag gtgaagaaaa tgaacatagg
     5161 aataatggga ttgggaacgt atacacccaa aacatttatc agcgcaaaag agatttctga
     5221 acagacggga ataccggagg atgtaatttc agtaaaattt ggagtaaaac agaagcctat
     5281 ccccggtcct gaggatacta caagtcatat gggaatagaa gctgcaagag ctgcaattgc
     5341 aaatgcaggt atttctgcac aggaaataga tcttattatt tggaatggag cacagcataa
     5401 ggattatcca tgttggcttg ccggnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5521 nnnnatctta ttatttggaa tggagcacag cataaggatt atccatgttg gcttgccggg
     5581 cttaaggttg ccgaagttct tggggctaaa aatgcatggt cgtttgatat ggaagccatg
     5641 tgcggttcaa tgatgagtgg tatggaagtt gcaaaatcaa tgatgacagc caatgaagat
     5701 attaaaacag tacttttggt aagcggatac aggaatcttg atcttataag ctacaaagtg
     5761 ccggaaacat cctttatgtt tgatattgga gccggcggtg ctgctatggt tttaagaaag
     5821 ggttatgaca gaaacgttat tctcggtacg gcgtttaagg gagacggttc cttttcagag
     5881 gactgtgttg ttgaaaaagg cggttccaag agctggccta tggctgaaaa ggattttgaa
     5941 aacatgcact ttgttgtcag agatcctgca acgtttaaag ccagacttgg agaaaagact
     6001 atgcctaact tttattgggt aataagacag gcattgaaaa aatcaggaaa tcttaccgat
     6061 aaggatatta attatcttgc cattcttcat ttcaagcgtt ccgcccatat ggaggttctc
     6121 aaagaacttg gtcttaaaga agatcagacc acatatcttg atgattacgg acatattgga
     6181 cagaatgatc aggttctttc tatcgagctt gggcttaaat ctggtaaaat aaaagatgga
     6241 gataacattg ttatggttgg agctggctta ggttttgtat gggccggtgc agtcgtaaga
     6301 tggggaaagg cactttaaaa atattctgga ggggataata ttatgagaat gaacggaaaa
     6361 gtttgcgttg taacaggtgg agcaagagga atcggaaaag aaatcgttac taagtttgca
     6421 aatgagggtg cacttgtagt atttgcatgt gatatgaaca aagaggcttt ggatgagctt
     6481 gaaaaggaat ttaaaaacgt taagggatat attctcaatg taacagacag agagggaata
     6541 aaggtatttg ttgaaaaagt aagagcagaa tatggtagaa ttgatgcact ggttaacaat
     6601 gccggaataa ccagggatgc cctgatacag aaaatgagtg aggaagattg ggatgcagta
     6661 ataaatgtaa accttaaggg tgtgttcaat atgactcagg ctattggtcc gcttatgatg
     6721 gaaaccggaa gcggttcgat agtaagcatc tcttcagtag tgggtttgga tggaaatgtt
     6781 gggcagacta actatgctgc aaccaaaggc ggagttgtag ccatgaccaa gacgtggtca
     6841 aaggagtttg ccagaaaagg cgcaaaagtc agagctaatg ccgttgcacc gggttttgta
     6901 agaacaccga tgacagataa ggttcctgag aagatcctgg aagatatgaa aaataaggct
     6961 ctactaaaaa gattgggtga ggctgaagat attgcaaatg cagttctttt cctttcaagc
     7021 gaagaagctt cttttgtcac aggacaggtt ctcagggtag acggtggtct tgtaatataa
     7081 actttcagga ggcggaatat gtctagttgg acaagcgtat ataataataa gaagcgttcg
     7141 gtggaagaag ccattctttc tctgcctaag aggtgtacag tggttgtggg tctggctgca
     7201 atggaagctc agggcctttt aagtaacctc cataagttca gggacaattt tgagtatata
     7261 aaggttctga cctgtcttaa tatgaaagat taccctttca tttcttcaaa agatagcgaa
     7321 ggaattcttg aaaatcatac atggttcatg agtgtgccta ccagaaatgc aagggattcg
     7381 gggcttaaaa cggttgatta tataccgaac aacctgcaca tgtctagtac tgataagttt
     7441 gtttccgaaa aggaagatgg taataagata gtgttttggg gcacagtaac tcctatgaat
     7501 gaagtatccg gtttcatgag tcttgggctt tcaaacgtct atgaaatgga tattcttgag
     7561 aaggccgact atacggttct ggaagtaaac gataaggttc catggacaca cggcgagact
     7621 caggttcata tagctcaggc tgatgtggta acggaatcaa gttgggccat tccggatctt
     7681 tctgttgtag aacctgctca gacagaaatc cagatagctc agtatattgc agatctggta
     7741 agagacggat ctactgtcca gatagggata ggcggtattc caaatgctgt agccaagctc
     7801 cttaagaata aaaaagatct cggagtccac acagagatgt ttacagaatc tatgattgag
     7861 ctttttgaaa acgggatcat
//
LOCUS       DAOSHR010000092         7816 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_8054_length_7816_cov_6.275351, whole genome shotgun sequence.
ACCESSION   DAOSHR010000092 DAOSHR010000000
VERSION     DAOSHR010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 7816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7816
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8054_length_7816_cov_6.275351"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="PLU28_06245"
     CDS             complement(<1..349)
                     /locus_tag="PLU28_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34700.1"
                     /translation="MRLRTKISIALIITSAIFSIFQVILFYISYLDQFDTAKKILKDK
                     NDFITEKFNNYIRTSSEIVDYFIAHISRNENTIDKEHIMSMFDDFVESSSYIASAFIG
                     LPDGSILVKPEFDI"
     gene            442..1320
                     /locus_tag="PLU28_06250"
     CDS             442..1320
                     /locus_tag="PLU28_06250"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HOG34701.1"
                     /translation="MRKLIGVIAVISIIIAAFGIRNTVIREREQKVKMIEVYGFEDGY
                     DFMIGKHEITYLQYERYTAELKDKDVSSHTVPGDRYPVKSISWIQAVNFCNWLSSKEK
                     LACAYDDDYNLIDSNGQATKDISKVIGYRLPTEREWEFAAVGATKTKGFKYSSSDDPD
                     QAGWYRYNSGEKYLIQSSDRATIISNGCKPHETGLKVANEIGLYDMSGNLWEWVQDYY
                     YEMKALKTDIFHDMPSSQTAYLRTIKGGSWFDGEESMEITSREGWNEYSSSSDTIGFR
                     LARTIIPVRKGIDKDD"
     gene            1313..2182
                     /locus_tag="PLU28_06255"
     CDS             1313..2182
                     /locus_tag="PLU28_06255"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG34702.1"
                     /translation="MIKHITAKNFISTVNGKDEWFGLRYNFNIYRGCIHQCIYCDSRS
                     ECYQIENFEDLLVKTNAPVLARKELSHKRTKGLIGTGSMSDPYNPAEKELLIMREVLK
                     VIQAFRFPVHILTKSDLVLRDQDIIKEIATIKAYVSFTVTTYDDELSKKIEPFAPISS
                     RRIAAMKQLAKSGINTGILLMPTLPYITDNEENIKKIMQIAVDNGASYVIASFGVTMR
                     DRQREYYYKKLEENFPSIKEKYIKKYGNYYSCRSENSRKLTSIYNEYTVKYNLKKRVE
                     PYNEVVENLRLFS"
     gene            complement(2179..3384)
                     /locus_tag="PLU28_06260"
     CDS             complement(2179..3384)
                     /locus_tag="PLU28_06260"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOG34703.1"
                     /translation="MIKKLLTADIPKFISIVSNAYPSMDAHKPEIRDIYMKKFEEICK
                     HAKEVKVYGCYREKELLGGMLIYDYKMNYYNHMIDVCGLGTVATDFIHKKEHVAKELV
                     SYFIDYGKKNKKDFLLLYPFNVMFYSKFGFCPGEKMDLYRVKPAHFSILKSKGIMRYL
                     EEPDTEDIVKFYNSFSQKVHGMILKTEMEKKAFFRNPEIRTVGFYDSEKNLCGYVRFS
                     FRKENAGNWLKNSLVIREMIYSDTACLKSIFSFFASQKDQIEFVEFTTVDRDIWRLFE
                     DIYYPGEVIIPSVYHNSNIQGVGLMYRVCSIESVLKKLPAPSDFCIEFIVHDPLSGKD
                     EHFTLGSNKKKVSVSMDLGSFSSWSIGSISLEKLVYLGKAFITDSCALKELNAVFSFL
                     KPPYCTTSF"
     gene            complement(3427..3918)
                     /locus_tag="PLU28_06265"
     CDS             complement(3427..3918)
                     /locus_tag="PLU28_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34704.1"
                     /translation="MKKVILSLISLFIGIVILGNVVKYTDLMTGKTTYSLVDNYLDFG
                     NSVLSIDIQFTVSVPSDYEEVLLSVVNTTDKITIDSKSLILQGGSFNKEYALYDFNII
                     RLDCCEGTETRDFLIPRSELIAWITSENPVITVLGNKINLYSKISDEVVFNIKDFYYN
                     YVE"
     gene            4032..4481
                     /locus_tag="PLU28_06270"
     CDS             4032..4481
                     /locus_tag="PLU28_06270"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOG34705.1"
                     /translation="MQIIIKEYEKGFSGYIKNIDRETFKECTKSDQQIDDTALNHGNK
                     VFIAFYGEKAVGFISLLKAETLNYSGYWIDLIAVLPQFRGHSIAGMLIKKAYDYVENK
                     NCEILTALIRVNNISSRKAFEKESFAPSENDFKIYSKDISRGGKNAI"
     gene            4471..5610
                     /locus_tag="PLU28_06275"
     CDS             4471..5610
                     /locus_tag="PLU28_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOG34706.1"
                     /translation="MQYRQMGKTNEKVSVLGFGCMRFQVKDGKNDQIDEEKAISQLRM
                     AIDNGVNYIDTAYPYHGGNSETAVGKALENGYREKVKLATKLPSWLIKSREDMDKYLD
                     EQLAKLQTQYIDFYLIHALRKEFWENLKKNYIFDFIEKALKSGKIKHIGFSFHDEYDV
                     FKEITDSYDWDFCQIQFNFMDTEYQAGLKGLRYAAEKGMGVVIMEPLRGGSLVNKVPP
                     QALEIYKNSGYSRTPAEWALRYIWDYKEVSVILSGMNEESHIIQNINTASQALAGSLT
                     EKEKNVIEEVKKVYLSKIKVNCTKCQYCMPCPFGVDIPGNFAMYNEYGIYDNYEDAKY
                     TYNEMSRLKKSSALCKECGRCEPLCPQSISIPQKLKEITKLFIGQ"
     gene            5618..6469
                     /gene="rfbD"
                     /locus_tag="PLU28_06280"
     CDS             5618..6469
                     /gene="rfbD"
                     /locus_tag="PLU28_06280"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HOG34707.1"
                     /translation="MILITGANGQLGKEFQKFLSEKNREVLALDIQELDITDLEKTRK
                     ILKNIQAEYIINCAAYNDVDKAQEEWKKAFSVNAIGVRNLAIVAAETGAELVHFSSDY
                     VFSGNRNVPYTIYDRPNPINKYGESKVLGERFLKELNNKYYLVRTSWLFGDGEKNFLR
                     KVLLWASSRQKIRVVDDEVSSPTCTSDLAKAVWDIIKIKAYGTYHITNSSCSRYEWAE
                     LILKEINWQGVLERSKRQEFNLPAKRPDYTVLDNFGFKETTGYEMPSWQEATIRFITT
                     LKTKGQI"
     gene            6593..6919
                     /locus_tag="PLU28_06285"
     CDS             6593..6919
                     /locus_tag="PLU28_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34708.1"
                     /translation="MVIDLEKLDESEKIVYGFYFNISDDPNDDIELSYLDGYREEAVE
                     EIEKVKNTGDIKKAQHLMDILKKSDDFFNEQKESIKKVFKDDKGVMESIENIEKEIKK
                     MNYRIS"
     gene            complement(7413..7489)
                     /locus_tag="PLU28_06290"
     tRNA            complement(7413..7489)
                     /locus_tag="PLU28_06290"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7453..7455),aa:Met,seq:cat)
     gene            complement(7493..7569)
                     /locus_tag="PLU28_06295"
     tRNA            complement(7493..7569)
                     /locus_tag="PLU28_06295"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7533..7535),aa:Met,seq:cat)
     gene            complement(7591..7679)
                     /locus_tag="PLU28_06300"
     tRNA            complement(7591..7679)
                     /locus_tag="PLU28_06300"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7643..7645),aa:Leu,seq:taa)
BASE COUNT         2885 a         1330 c         1415 g         2186 t
ORIGIN      
        1 ttatgtcaaa ctcaggcttt actaagatac tcccgtccgg aagtcctata aatgctgatg
       61 caatatatga agaagattcg acaaaatcat caaacattga catgatatgc tctttgtcta
      121 tagtgttttc attgcgtgat atatgagcta tgaagtaatc tactatctca gaagaagttc
      181 ggatatagtt attgaacttc tcagtaataa aatcattttt atctttcagg attttctttg
      241 cagtgtcaaa ctggtcaaga taagaaatat aaaaaaggat aacctggaag attgaaaata
      301 tggcagaagt tattattaaa gctatagata tctttgtgcg cagtctcata gaccacctct
      361 tgtatgtaag ataacgtcaa aataaaggtt ctaatattaa tatattatat catacttaac
      421 cttaaaaatc aggagaaaaa tatgagaaaa cttattggtg ttattgctgt tataagtatt
      481 ataatcgcag cctttggtat acgaaatact gtgatcaggg aaagggaaca gaaagtgaag
      541 atgatagaag tatatggttt tgaagacggt tatgacttta tgataggaaa acatgagata
      601 acgtaccttc aatacgaaag atataccgcc gaactaaaag ataaagatgt ttcttcacac
      661 actgtaccgg gagacagata tccagtaaaa tccataagct ggattcaagc agtcaatttc
      721 tgtaactggc ttagcagcaa ggagaagctt gcgtgtgcat acgacgatga ctacaactta
      781 atagactcga acgggcaagc aacaaaagat atttctaaag ttattgggta caggctgccg
      841 acagaaagag aatgggagtt tgccgctgta ggagcaacga aaaccaaagg ttttaagtac
      901 agttcttcgg atgatcccga ccaagctgga tggtatcgtt acaacagcgg agaaaagtat
      961 cttattcagt cttccgaccg tgcaaccata atatcaaacg gctgcaagcc acatgaaaca
     1021 ggattaaaag ttgccaatga aataggatta tatgatatga gcggtaatct gtgggaatgg
     1081 gtccaagact attactatga aatgaaagct ttaaaaacag atatatttca cgatatgcca
     1141 tcctctcaga cggcttatct aaggacaatc aaaggtggct cgtggtttga cggcgaggaa
     1201 agtatggaaa taacaagtag ggaagggtgg aacgagtaca gcagctcatc tgatacgata
     1261 ggatttcggc tggcaagaac tataattcca gtcagaaaag ggattgacaa agatgattaa
     1321 acatataaca gccaaaaact ttatatcaac ggttaacggg aaagacgaat ggtttggctt
     1381 aaggtacaat ttcaatatat acaggggctg catacatcaa tgcatatact gtgattcaag
     1441 aagtgaatgt tatcagatcg aaaattttga agatcttttg gtaaaaacaa atgcccctgt
     1501 tcttgcaagg aaagaacttt ctcacaaaag aacaaaaggt cttataggaa ccggttcaat
     1561 gagtgatccg tataatcccg ctgaaaaaga acttcttatc atgagagagg tcctaaaagt
     1621 aattcaggct ttcagatttc cggttcatat tcttaccaaa agcgatcttg tacttaggga
     1681 tcaagatata atcaaggaaa tagccacgat aaaagcctat gtatctttta ctgtaacaac
     1741 gtatgatgat gaactaagca agaaaattga accatttgct ccaatatctt caagaagaat
     1801 tgccgcgatg aagcaactgg caaaaagcgg tataaataca gggattttac ttatgccaac
     1861 cctgccgtac attacagaca acgaagaaaa tataaaaaaa ataatgcaga tagcggtgga
     1921 taacggtgca agctatgtaa tagcaagctt tggagttaca atgcgcgata gacagcgaga
     1981 atactactat aaaaaactag aagaaaactt tcccagtata aaggaaaagt atataaaaaa
     2041 atatgggaat tactattcat gcagatcaga aaactccaga aaattaacca gcatatataa
     2101 tgagtatact gtgaaataca acctcaaaaa aagagtagaa ccgtataacg aggttgtaga
     2161 gaatcttaga ctcttttctt aaaatgaagt tgtacagtaa ggaggtttca agaaagaaaa
     2221 aacagcattc agctccttca gcgcgcaact atcagtaata aaggcctttc caaggtagac
     2281 aagcttttca aggcttatgc tccctatgct ccagcttgaa aatgaaccca agtccattga
     2341 tacagatacc tttttcttat tgctgcctaa agtaaagtgt tcatcttttc cggataaagg
     2401 atcatgaaca atgaattcaa tgcaaaaatc agaaggagca ggtaactttt taagaacaga
     2461 ctcaatactg cacactctgt acataaggcc tacaccctgt atatttgaat tatggtagac
     2521 acttggtata atgacttctc ccggataata aatatcctcg aaaagacgcc atatatccct
     2581 atcaacagtt gtaaactcta caaactctat ctggtctttc tggctagcaa aaaatgaaaa
     2641 tatggatttg agacacgcag tatcagaata aatcatctct cttattacaa gactgttttt
     2701 aagccaattt cccgcatttt cttttctgaa agagaacctc acataaccac aaagattttt
     2761 ttcagaatca taaaaaccaa ctgtccttat ttcaggattt ctgaaaaaag cttttttctc
     2821 catctcagtt ttaagtatca taccatggac tttttgagaa aaagagttat aaaacttgac
     2881 tatatcttca gtatccggtt cttcaagata acgcataatg cctttgcttt ttaatatact
     2941 aaaatgagct ggctttactc tgtacaaatc cattttttct cccgggcaga aaccaaactt
     3001 actgtaaaac attacattga aaggataaag cagcaaaaaa tcctttttgt tttttttacc
     3061 atagtctata aaataactta caagctcttt agccacatgc tctttcttat gtataaaatc
     3121 tgtagccaca gttcctaggc cgcatacatc aatcatatgg ttataataat tcattttgta
     3181 atcatatatc agcataccac ccaaaagctc tttttccctg taacatccat atactttcac
     3241 ttctttagcg tgtttacaga tttcttcaaa ctttttcata tatatatcgc gtatctctgg
     3301 tttatgtgca tccatcgaag gataagcatt tgaaacgata gaaataaact ttggaatatc
     3361 ggctgttaga agcttcttta tcataaattc ccccctaaaa gtagtattta ctaagttttt
     3421 ccccttttat tcaacataat tataatagaa atctttaata ttaaatacta cttcatcgga
     3481 tatttttgaa tataagttaa ttttatttcc caaaactgtt ataacagggt tttccgaagt
     3541 tatccaagct ataagttcag atcttggaat aagaaaatct cgtgtctccg ttccctcaca
     3601 acagtcaagt ctgataatat taaaatcata aagagcatat tccttattaa aacttcctcc
     3661 ctgaagaatc agactctttg agtcaatggt gatcttatca gtggtattca caacactcag
     3721 cagaacttcc tcataatcag acggaacgga aacagtaaat tgaatatcaa tggaaagtac
     3781 agaatttcca aaatcaagat aattatccac caaagaataa gtagttttac cggtcattaa
     3841 atcagtatac tttacaacat ttcccaaaat aacgattccg ataaacaatg aaattaacga
     3901 cagaataact ttcttcataa ccttcgcctc cttttttatt ctacaaatat attatataat
     3961 taattatgaa aaagatttat taacaaaaga taaaattaga taaattttat cggttttgcg
     4021 taggagaaaa aatgcagata ataataaaag agtatgaaaa aggtttttct ggatatataa
     4081 aaaatattga cagagagact tttaaagaat gtaccaaatc cgatcagcag attgacgata
     4141 cagccttaaa tcacggaaat aaagtattta tagccttcta tggagaaaag gcagtagggt
     4201 tcatctcgct tttgaaagca gaaacactta actattctgg atattggata gaccttatag
     4261 cagttttacc tcaattccga ggacattcaa tagcgggaat gcttataaaa aaagcatatg
     4321 attacgtaga aaacaaaaat tgtgaaatac tgactgcact catacgggta aataacattt
     4381 cttcacgaaa agcatttgaa aaagagagtt ttgctccttc ggaaaatgat tttaaaatat
     4441 acagcaaaga catcagtcgg ggaggtaaaa atgcaatata gacaaatggg aaaaacaaat
     4501 gaaaaagtat cagttttggg ttttggttgt atgagatttc aggtcaaaga cggaaaaaat
     4561 gaccagatag atgaagaaaa agccatatct cagttgcgta tggctattga taacggtgta
     4621 aactatattg atacggcgta tccttatcat gggggcaaca gcgaaaccgc agtaggaaag
     4681 gcgcttgaaa atggctacag agaaaaagta aaactagcaa ccaaacttcc ttcctggttg
     4741 ataaaatcac gagaggatat ggacaagtac cttgatgaac aattagcaaa actacagacc
     4801 cagtatattg acttttacct tattcatgcc ttaagaaaag aattttggga aaaccttaaa
     4861 aagaactata tctttgattt tattgaaaaa gcacttaaga gcggaaaaat aaagcatata
     4921 ggattttctt tccatgatga atatgatgtt tttaaagaaa taactgattc atacgactgg
     4981 gatttctgtc agatacagtt caattttatg gatacagagt accaggcggg acttaaagga
     5041 ctcagatatg ctgctgaaaa aggcatggga gtagtgataa tggaacctct gcgcggtgga
     5101 agcttagtta ataaagttcc tcctcaggca ttagaaatat ataaaaattc cggatactcc
     5161 agaacccctg ctgaatgggc actccgttat atatgggact ataaagaagt cagcgttata
     5221 ttaagcggca tgaatgagga atctcacata atccaaaaca taaatacagc atcacaggct
     5281 cttgcgggca gccttacgga aaaagaaaaa aacgttattg aagaagtaaa aaaagtatac
     5341 cttagcaaaa taaaagtaaa ctgtactaag tgccaatact gtatgccgtg tccgtttggc
     5401 gtggatattc caggaaactt tgctatgtac aacgaatacg gaatatatga caactatgaa
     5461 gatgcgaaat acacatataa tgagatgtct aggcttaaga aaagctctgc cttatgtaaa
     5521 gaatgtggaa ggtgtgaacc gttatgtccg caatcgatat ctattcctca aaagctcaaa
     5581 gaaataacaa aacttttcat aggacaatga aaataatatg atacttataa caggagcaaa
     5641 tgggcagtta ggaaaagaat ttcagaagtt tctttcagaa aaaaacagag aagtacttgc
     5701 cttggacata caggaacttg atataacaga tttagaaaaa acaaggaaaa tccttaaaaa
     5761 cattcaagca gaatacataa taaactgtgc tgcatataat gacgtggata aagcccaaga
     5821 agaatggaaa aaggcatttt cggtaaatgc aataggagta cgtaacttag ctattgttgc
     5881 agcagaaaca ggcgcagaac tagttcattt ttcatcagac tatgtatttt ctggcaacag
     5941 aaacgtaccc tatactatat atgaccgacc aaatcccata aacaaatatg gcgaaagcaa
     6001 agttcttgga gaaagattct taaaagaact caataataaa tactacctag taagaacaag
     6061 ctggcttttt ggtgacggag aaaaaaattt tctccgaaaa gtacttttat gggcatcatc
     6121 cagacaaaaa ataagagtag tggacgatga agttagctct ccaacgtgta cttctgacct
     6181 tgcaaaagca gtctgggata taattaagat aaaggcttat ggtacgtacc acataacaaa
     6241 ctcatcatgt tcaagatacg aatgggcaga gcttatactt aaagagatca attggcaggg
     6301 agtattggaa agatcaaaaa gacaagaatt taatcttccc gccaaaagac ctgactatac
     6361 agtacttgat aactttggat tcaaagaaac aacaggatat gaaatgccgt catggcagga
     6421 agcaaccata agatttatca ctacactaaa aactaaagga cagatctgat aagaacacta
     6481 agtgcatgag aattcataat tgaggacaaa aaatattttg tttcggtaga tataaaaggg
     6541 aactcaataa aaactttcat cagaatgagt gagaaaaagg aggtcgtcaa atatggtaat
     6601 tgaccttgaa aagttagatg aatctgaaaa aatagtttat ggtttttatt ttaatatttc
     6661 tgacgaccca aatgatgata tcgaactatc ttatttagac ggatacagag aggaagcagt
     6721 cgaagaaatc gaaaaagtaa aaaatacagg agatattaaa aaagcacaac atttaatgga
     6781 tattttgaaa aaatctgatg atttttttaa cgagcagaaa gaatctataa aaaaggtatt
     6841 caaagatgat aaaggtgtca tggaaagtat tgaaaacata gaaaaagaaa taaagaaaat
     6901 gaactacaga ataagttgat ataataaaaa aagagagctt aaaggctctc tttttttatt
     6961 cctaatccaa ggttctactt gaagatacag gttgtttcaa aaaccttata ggtatgctag
     7021 gaagtagaac caaaatggaa atatgagggt ttattgaact agtttcaaaa ccttatagtt
     7081 aagaaaaaaa taataacaat aataccttag ctgttgattt tctctaccaa agtcagcagt
     7141 ttaactcaca ttaaaatcat ttgatcccct tctggtatgg actcaatgat ttgcactttc
     7201 tcatcttcaa aaatattgac agaaagtttt atccaactta ccaacgaatc actcctttat
     7261 aagaatagtt atatattcaa tttttgatat aaaaaaacca gctcttgtaa aaaatgaact
     7321 ggcataaatg aaaaagcgat caggagcgta aatccataaa tgataattca cgcttctaac
     7381 cgcttcacac tcttggtact acggaaaatg actggtggcg gcgaagagat tcgaactccc
     7441 gacactgcgg gtatgaaccg catgctctag ccaactgagc tacaccgccg tatggtagcg
     7501 gggacaggat ttgaacctat gaccttcggg ttatgagccc gacgagcttc cagactgctc
     7561 caccccgcac cgtgtagtat ttcccgtacc tggtgcctag ggtcggactc gaaccgacac
     7621 gagtgttccc acccagtgga ttttaagtcc acagcgtcta ccaattccgc cacccaggca
     7681 acagttacgt aagatatctt accataatta tttcaggttg tcaaggaatt tcttcgatgt
     7741 tttcagaaat taatatttgt ttaggaaaat ttagcatgaa cttagtgatg cttccgtaat
     7801 attcaagttt tccttt
//
LOCUS       DAOSHR010000093         7798 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_8082_length_7798_cov_5.834689, whole genome shotgun sequence.
ACCESSION   DAOSHR010000093 DAOSHR010000000
VERSION     DAOSHR010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 7798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7798
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8082_length_7798_cov_5.834689"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            227..1621
                     /locus_tag="PLU28_06305"
     CDS             227..1621
                     /locus_tag="PLU28_06305"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HOG34709.1"
                     /translation="MTKSELDRIFEDQKRFFETGYTKDVKNRVELLSKLKKKVSDSED
                     LICSALEKDLGKSRTETFLTETYMLMTEINYFMKNIPALCAPKKVKTPLSAFPGKSLI
                     FAEPYGNVLIISPWNYPLQLALAPLVGAVAAGNCVILKPSEYAPHTATVLEKIISGVF
                     LKEHVCTVCGGIEETSYLLEKPNDYIFFTGSPQVGKVVMEKASVNLTPLTLELGGKSP
                     CIIDRTADITLSAKRIAWGKFINAGQTCVAPDYILIEEEIKEQFIKELVKYIELFYAS
                     VDKDHPNGYAQLPRIINEKHFSRLEGYIKNQKVIYGGICEREKLFISPTVIENPSLDS
                     PVMQNEIFGPILPIIEVANREKAREIIQKYPKPLALYIFSNDPEFVSTSVNTISSGGA
                     CINDTISHITSEYLPFGGVGNSGMGSYHGKSSFDTFTHYKSVLFKGKFDIKEKYPPYS
                     EKFLLNLIKKTVSK"
     gene            complement(1657..3381)
                     /locus_tag="PLU28_06310"
     CDS             complement(1657..3381)
                     /locus_tag="PLU28_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34710.1"
                     /translation="MKKLLVLILLIIISFSSFAQSYGLTIAGGAAYVFKSMGFLQALV
                     EEGFFPQEYYTTSMGGFPGYFMAAGIEPKSVYEVFKIIDFEEMFDLSFPLNGGFVNSQ
                     RLMDLVYAVSGVSNFSQFKYPLHLGVLNLNTWEDKYISEGDALKALAASTALEVFFEV
                     VRYPDGSYSDIGPKDLMVKYPKDRFGTDKTFLMRITPNPRIVTSRDYSSIINIAYLLI
                     DIGGFYANNLYSADKIDYDHIFNMDVSSEIDPKNFSKAIELYDIGYIEGKEWVKNNDI
                     FSQYSIEKNSIEPKEIDYKKIYTDFKTESIYRPRDFYYTFKISPVLKENMMMRAFIYS
                     EFGDFRLSAGEIVKYDFSLDTFINGKLYYFPFENSRISFDYIIDDSFRIDFKNYFLKE
                     YKNLFSVNLGHYNKFSKLWYNFADFRYDTTVSNRLEENGCFFDSRFSTDFEKFNYEFQ
                     AGSINQATSATFYSSLGYAYGDEMGRVNPDTVFGSFDQKVYLNEKIKVSLMKDMNFDI
                     AQFIYLNDAYVFLDYFLTVDDFKKINHTIKAGFELPFSFGGVFRIPVEMSVKYKDGFQ
                     FSLTLQTE"
     gene            complement(3767..4849)
                     /locus_tag="PLU28_06315"
     CDS             complement(3767..4849)
                     /locus_tag="PLU28_06315"
                     /inference="COORDINATES: protein motif:HMM:NF022594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2920 family protein"
                     /protein_id="HOG34711.1"
                     /translation="MAESVIRVRSVRDEIYKKDRSFNLHCYLPDAGVNEKTGLMGIHT
                     GYCLNPENVFFKKMRKAYAERFNVITFTAAYMGVEASLNRKLIFNSERSKITLQDIKT
                     LTKPDRCIDLSHELFLDEKFEGLSDPQKTDYVDYGFCQSLDIANGLYYLIHNYSINTK
                     RIYLLGSSLGSYLSHMVMKFLPKSVKGIIDISGLVGIEEERITNGRYCKAFLPNYFIS
                     MKTHSLYEEISQQQIDIRNLIMPEHFSPSQAKVVMLSGADDIMVPVDKKMQYSKVLSE
                     RGIDNLLKIIDDSSVDGKLVTHSGHSLGSNILGIFDSLFPQFEELFSQNDGMVEKSGD
                     AYRYKVYGGYYETVMNEFIDIKYHES"
     gene            complement(4842..5075)
                     /locus_tag="PLU28_06320"
     CDS             complement(4842..5075)
                     /locus_tag="PLU28_06320"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HOG34712.1"
                     /translation="MSLFRNEFKTFFSMEIDENNINKKVLELNIDSLSFWEFIGILEE
                     KYGIEFDMLELSTSIDSIGGLETLFMKKVKKDG"
     assembly_gap    5231..5330
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5331..6639)
                     /locus_tag="PLU28_06325"
     CDS             complement(<5331..6639)
                     /locus_tag="PLU28_06325"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOG34713.1"
                     /translation="MRIFEYDADSDRMNIFDEKIFFSTVKYLQEKFKREYCVRAGSIV
                     AVYLPTSAFTNAVVLALYKMGAVVSNLFSSYGDQALKTRLELLNPDYIISWRSISFKL
                     NKIREIKAESLLSSDIPIVSREDVVITSTPDLAFIHFTSGTTGMPKMVPHSAKDLNGL
                     KISIEKVFDIKAGTNYLCTADFGWVTGFYYGVFMPLISNLNFFIIKNMNGFRLMKALS
                     RFREWNIEVIYTSPSFLNIAYRFFRKYETNIKQVYTVGEKLPIETKKKYLELGYEITD
                     TWWQTELGCISISGRVKAQTSFPLGTMIVQDYIGKPLDFVDIALLNDDSIVPIQSILR
                     MPPAEMTGELLVNRITHPSIMKEYYKADENARKKFIGDYYKTGDLIRLLPETEDPQSY
                     NIQYISRADDIIVIAGELISPDEIESLVMKKFDSISECCAVAAD"
     gene            complement(6648..>7798)
                     /locus_tag="PLU28_06330"
     CDS             complement(6648..>7798)
                     /locus_tag="PLU28_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34714.1"
                     /translation="YESVLKQIMLINPSFEFKYEDIEIFVKACIKSAMFCRLIEYATR
                     FLTKNAESMNFFILSVEVNKKELLSDEKTELYRAFSESKGEYSNLNKIRYDFYTGAPG
                     IKSLLQQAQEYTDFNNSVDFYADIIYFMLKLGMPFNTFIEKTVEKNIEKFFQYIVNNY
                     EDINELILRYLQGTEKNSSFYDIKLRKLLCETLLLNKKDFTEEEFDMVTSEYFSSGVE
                     YIKNLYSPTIIDNEYLQELKNNEEKFFLFLAKAYQNEESKLKEYCDEAVRHYPIMKNA
                     IEYRLKKETQIKSSHDLSCGQRLPADELQLHKLISRADNLVMDGQYYDALTLYKQILS
                     LISLSEKKASSFKESPLSFEIEKKISDLKSRYSNEIIFQKNSHGSSER"
BASE COUNT         2395 a         1566 c         1306 g         2431 t
ORIGIN      
        1 gtatattata tcatatttgg gcgaatattg catgaggttt ttagagatgc ccttattata
       61 ttatacatca atgtaactaa taaaaataga gtggaaatta tcatttttaa ctgattatca
      121 ttgtttaaaa aagaccttta tctttttaat atattttttt aaactttttg caactattaa
      181 aaaattctta taagataatt attttatctt ccgcaggagg aattttatga ccaaaagtga
      241 gcttgacaga atttttgaag atcagaaacg tttttttgaa accggttata ctaaagatgt
      301 gaaaaataga gtagaactgc tttctaagct aaaaaagaaa gtatctgact ccgaagatct
      361 tatatgctca gctcttgaaa aagatcttgg aaaaagcagg acagaaacct ttcttacaga
      421 aacctatatg ctcatgacag aaataaatta ttttatgaaa aacatacctg ctctttgcgc
      481 accaaagaaa gtaaaaactc ctctctcggc ttttcccggc aaaagcctaa tatttgctga
      541 accttacggt aatgttctta ttatatctcc ctggaactat ccactgcagc ttgcgcttgc
      601 tcctcttgtt ggagctgttg cagcaggcaa ctgcgttata cttaaacctt cggaatacgc
      661 accgcacaca gctactgtgc tggaaaaaat tatttccgga gtatttctca aagagcatgt
      721 atgcacagtc tgcggtggca ttgaggaaac ctcatatctt cttgaaaaac caaatgatta
      781 catttttttt acaggcagcc ctcaggtagg aaaagttgta atggagaaag catcggtgaa
      841 tctcactccg ttgactcttg aactgggtgg aaaaagcccg tgcataatcg accgcacagc
      901 cgatattact ctgagtgcca aaagaattgc ttggggaaag tttataaatg caggtcagac
      961 atgtgtagcc cctgattata ttctgataga agaagaaata aaagaacagt ttataaaaga
     1021 acttgtaaag tatatagaac ttttctatgc ttctgtggat aaagaccatc caaatggcta
     1081 tgcacaactt cccagaatca taaacgaaaa acatttctca cgattagaag gatacattaa
     1141 aaatcagaag gttatctatg gcggtatctg tgaacgcgaa aaacttttta tatcgcctac
     1201 agtcatagaa aatccttctc ttgactcccc tgtaatgcaa aatgaaatct ttggacctat
     1261 tctacctata atagaagtag ccaacagaga aaaagccaga gaaataatac agaagtaccc
     1321 aaaacctctt gcattgtata ttttctcaaa cgatcctgag tttgtatcaa catcggtcaa
     1381 tactatttca agcggcggag cctgcataaa cgacactata tcgcatatta cttcagagta
     1441 tctgcccttt ggcggagtag gaaacagcgg gatgggaagc taccatggca agagctcctt
     1501 tgataccttt acccattata agagcgtgct tttcaaggga aagttcgata taaaagaaaa
     1561 gtatcctcct tatagcgaaa aatttttact taacctaatt aaaaagaccg tatccaagta
     1621 agaaaaaaga aaactcccca tatgaagggg agttttttat tctgtctgaa gggttagact
     1681 aaactgaaaa ccatctttat acttaacgct catttcaacc ggaatcctga aaactccgcc
     1741 aaaagaaaaa ggaagctcaa agccagcttt tatagtatga ttaatttttt taaaatcatc
     1801 caccgtcaga aaataatcta aaaaaacata cgcatcattc agatatatga actgagcaat
     1861 atcaaagttc atatctttca tcaaagaaac ttttatcttc tcgttcagat aaactttctg
     1921 gtcaaagctg ccaaataccg tatcgggatt gactcttccc atctcatcac cataggcata
     1981 gcctaagcta gaatagaagg tagcacttgt agcctgattg atacttccgg cttgaaactc
     2041 ataattgaac ttttcaaagt ctgttgaaaa cctgctgtca aaaaagcatc cgttttcttc
     2101 aagcctgttt gaaacggttg tatcatagcg aaaatctgca aagttgtacc aaagcttgct
     2161 aaacttattg tagtgcccca agttaacgga aaaaagattt ttatattcct tcaaaaaata
     2221 gtttttgaaa tcaatcctga aggaatcgtc tattatgtag tcaaagctga ttctgctgtt
     2281 ttcgaacgga aagtaataaa gctttccgtt gataaaagta tcaaggctga aatcatactt
     2341 cacaatttcc ccggcagaaa gcctgaaatc cccaaattca gagtagataa aagctctcat
     2401 catcatgttt tctttcagga cgggagaaat tttaaatgta taataaaaat cacgcggcct
     2461 gtatatggac tcggttttga aatctgtata tatcttcttg taatctatct ccttaggctc
     2521 aatactgttc ttttctatag aatactgaga aaatatatcg ttgttcttta cccattcctt
     2581 tccttcaata tagcctatgt cgtaaagctc tatggctttt gaaaaatttt tgggatctat
     2641 ctcactactg acatccatat tgaatatgtg gtcatagtct atcttatctg ccgaataaag
     2701 attattggca taaaaacccc caatatctat aagaaggtag gctatattta taatactgct
     2761 gtaatccctg ctggttacaa ttcgcggatt gggagttata cgcatcagaa acgtcttgtc
     2821 agttccaaaa cggtctttag gatacttaac cataagatct ttcggcccta tatcagagta
     2881 agagccatca ggataacgta ctacctcaaa aaaaacctca agcgctgtag atgcagcaag
     2941 cgcttttaaa gcgtctccct cagaaatata cttgtcttcc cacgtattaa gattgagaac
     3001 gccaagatga agcggatatt taaactgact aaagttactt actccgctga cagcatacac
     3061 cagatccatg agcctttggg agttgacgaa tcctccgtta agaggaaagg aaagatcaaa
     3121 catctcttca aaatctatga ttttaaaaac ttcataaaca gattttggtt ctatccccgc
     3181 agccataaag tatccgggaa atcctcccat ggatgtggtg taatactcct gcggaaaaaa
     3241 gccttcttcc accagcgcct gcagaaatcc catagatttg aatacataag cggctccccc
     3301 tgctatggtc agaccatagc tctgtgcgaa agatgaaaat gaaatgatta taagcagtat
     3361 aagaaccaat agttttttca ttaaaacacc tctgttttat agataaaccg tttactacag
     3421 tcttctttaa ttatacactt gtatgttacg cctgatttaa tttcatgaaa aactttctaa
     3481 acatgcagca gaacatgtcc tgctgataat tttccataga catgcaaatg tttcttaaaa
     3541 gaaattcatg tgtaacatta gaagttttat ctattctggg gaagaactct ctttgagagg
     3601 gttgaaaaga tcgttttcaa caaaaaatac aaaagttcaa caaaaattgc aactgctgtg
     3661 cagaatatag tgaacataca tacacttcgg ccaacttgtc ttgttttctt actatgactc
     3721 aaaaagtcct ccctgaagga ggacttttac tatcttttac agttgttcag gattcatggt
     3781 attttatatc aataaactca ttcattacag tttcatagta gccgccatat accttgtaac
     3841 gataagcatc cccggatttt tcaaccatac cgtcattctg ggaaaagagt tcttcaaact
     3901 ggggaaacaa gctgtcaaat attcccaaaa tatttgaacc caatgagtgg ccgctgtggg
     3961 ttacaagctt accgtcaacc gaagagtcat cgattatctt aagcaggtta tctatccctc
     4021 tttctgacag aactttactg tactgcattt ttttgtcaac cggaaccatt atatcgtctg
     4081 caccgcttag cataaccact tttgcctgcg aaggggaaaa atgctcaggc atgataaggt
     4141 ttcttatatc tatctgctgc tgagagatct cctcatacaa agaatgtgtc ttcatactta
     4201 taaagtaatt gggcagaaac gctttacagt atcttccgtt ggttattctt tcctcttcta
     4261 ttccgaccag accacttata tcgattattc ccttaacgga tttaggcaga aatttcatga
     4321 ccatatgcga cagataactt cccagagagc ttcccagaag atatatccgt ttagtattta
     4381 ttgaatagtt atgaatcaga tagtacagtc cgttggctat gtccaacgac tgacagaatc
     4441 cgtagtcaac ataatcggtc ttttgtggat ccgaaagacc ctcaaacttc tcatccagaa
     4501 aaagttcatg actcagatct atgcacctgt cgggcttagt aagcgttttt atatcttgca
     4561 gagtgatttt tgatctttca gagttgaaaa tcagctttcg gttcaagctg gcctcaactc
     4621 ccatatatgc ggcagtaaag gttataacgt tgaatctttc tgcgtatgcc ttcctcatct
     4681 ttttgaaaaa aacattttca ggattaagac agtatccggt atgaatgccc attaagcctg
     4741 ttttttcatt tactcctgca tccggaagat aacaatgaag attgaaggac ctgtcttttt
     4801 tataaatttc gtccctgaca gatcttactc tgataaccga ctcagccatc tttttttacc
     4861 tttttcataa aaagtgtttc aagcccgccg atactgtcta tagatgtcga tagttccagc
     4921 atatcaaact caatcccata cttttcttca agaataccta tgaactccca gaagctcaaa
     4981 gagtctatgt taagctctag aactttttta tttatattgt tttcatcaat ttccatggaa
     5041 aaaaaagttt taaactcatt tctgaataag ctcattggcc atcctcctca gctcgtttct
     5101 ttttatcttt ccgctttctg tcttgggaag agctccgatc tttacaacct tgtaaggaac
     5161 cattgagggc ataaggtttt gagcaatagc tttttttatt tgttcctgtg taatctcgga
     5221 tgcacaagag nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tatccgccgc
     5341 tacggcacag cattcgctta tactgtcaaa tttcttcatg accagcgatt caatttcatc
     5401 cggagatata agctctccgg caattactat gatatcgtct gcccgtgaaa tatactgtat
     5461 gttatagctt tggggatcct cagtctcggg taaaagtctt ataagatctc cggttttata
     5521 gtagtctcct ataaattttt tacgggcatt ttcatcagct ttataatatt ctttcattat
     5581 acttggatgc gttatacggt tcaccagcag ctcccctgtc atttctgcag gaggcatccg
     5641 cagaatactc tgtatcggta ctatactatc gtcattaagc agtgctatat cgacaaagtc
     5701 aagcggtttg cctatataat cttgaacaat catggttcct aatggaaatg aagtttgagc
     5761 ttttaccctg ccgctgatac ttatacagcc aagctctgtc tgccaccatg tgtcggtaat
     5821 ttcataccca agctccaagt actttttttt ggtctctatg ggcagcttct ctcctaccgt
     5881 atatacttgc tttatattgg tctcatattt tctgaaaaac cggtaagcta tgttaagaaa
     5941 actcggagaa gtatagatca cctcaatatt ccactccctg aacctgctca gagccttcat
     6001 aagcctgaac ccgttcatgt tcttaataat aaaaaagttc aggtttgaaa taagaggcat
     6061 gaatactccg taataaaatc ccgtaaccca tccaaaatca gccgtacaaa ggtagttagt
     6121 cccggctttg atatcaaaga ccttctcaat agaaattttc agaccattaa ggtcttttgc
     6181 agagtgcgga accatcttag gcattcctgt agtccccgaa gtaaaatgta taaatgccag
     6241 atccggcgtg gaggtgatca caacatcctc tcgtgaaaca ataggtatgt cggaacttaa
     6301 aagactttca gcctttattt cacgaatttt attaagtttg aagcttatac tgcgccatga
     6361 aataatataa tccgggttaa gaagctccag cctggtttta agagcctgat ccccgtaaga
     6421 agaaaacaga ttgctgacca cagcacccat tttgtaaaga gccaagacaa ccgcattggt
     6481 aaatgccgat gtaggaaggt aaacagctac aatactgcca gctcttacac agtactctct
     6541 cttgaatttt tcctgcagat acttaaccgt tgaaaaaaag atcttttcat caaaaatatt
     6601 cattctgtca gaatcggcat catactcgaa tattctcata aaagtcttta cctttcgctg
     6661 cttccgtgac tgtttttttg aaagattatc tcgtttgaat accttgattt cagatcggaa
     6721 atcttttttt ctatttcaaa agataacggt gattctttaa aagaagaagc tttcttttca
     6781 gataacgaaa ttaaagaaag aatctgttta tacagagtca gcgcatcata gtactgaccg
     6841 tccattacaa ggttatcggc acggctgata agcttgtgca gctgcagttc atccgcaggc
     6901 agtctttgcc cgcaagaaag atcatgactg ctctttatct gtgtttcctt tttcagacga
     6961 tactctatgg catttttcat tatgggataa tgtcttacag cttcatcaca gtactcttta
     7021 agcttactct cttcattctg ataagccttg gcaagaaaca gaaaaaactt ttcctcattg
     7081 tttttaagct cctgcagata ctcattatct attatagtag gtgagtataa gttttttata
     7141 tactcaactc ctgatgaaaa atactccgat gtgaccatat caaactcttc ctcggtaaag
     7201 tcttttttat tcagaagaag cgtttcgcaa agaagctttc ttaatttgat atcgtaaaaa
     7261 ctgctgtttt tttcagttcc ttgcagatac ctaagaatga gttcatttat atcttcataa
     7321 ttgttcacga tatactgaaa aaatttctca atgttttttt cgacagtttt ttctataaac
     7381 gtattaaaag gcatacccag cttaagcata aaataaatta tatcggcgta aaagtcaacg
     7441 gaattattga agtccgtata ctcttgtgcc tgctgaagaa gtgattttat acccggagct
     7501 ccggtataaa agtcatacct tatcttattc agattggagt actctccctt tgattctgaa
     7561 aaagcacggt acagctccgt cttttcatcg gacaaaagct cctttttatt tacctccacc
     7621 gaaagaatga aaaaattcat actttctgca ttttttgtaa gaaatctggt tgcatactct
     7681 ataagtcggc agaacatagc acttttgatg catgctttta cgaagatctc tatatcctcg
     7741 tatttgaact caaatgaagg attaataagc ataatctgtt tcaagacact ttcatagt
//
LOCUS       DAOSHR010000094         7728 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_8164_length_7728_cov_5.265216, whole genome shotgun sequence.
ACCESSION   DAOSHR010000094 DAOSHR010000000
VERSION     DAOSHR010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 7728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7728
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8164_length_7728_cov_5.265216"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /locus_tag="PLU28_06335"
     CDS             <1..562
                     /locus_tag="PLU28_06335"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HOG34715.1"
                     /translation="LDSSKIDIIELNISCPNVKEGGMNFGIKTQSAVQVLEQVRKAVS
                     KPLIVKLSPNAEDIVDMAVTCEKNGADGISLVNTFKAMAIDIYKRKPIFKNITAGLSG
                     PCIKPIALRMVYEVSKAVNIPVIGMGGISCWQDAVEFIMAGACAVQIGTMNFIKPDIC
                     LDIINGIEMFMEKNNFNSISEMKSII"
     gene            572..1135
                     /gene="pyrE"
                     /locus_tag="PLU28_06340"
     CDS             572..1135
                     /gene="pyrE"
                     /locus_tag="PLU28_06340"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOG34716.1"
                     /translation="MDINEILKETGALLQGHFLLSSGKHSNKYVQCAKLMMYPEKTQT
                     VLNQVAQKLKNLEFDIIVGPAIGGIVPAYELARLTGKICMYAERENDVMTLRRGFELF
                     PGQKVIICEDVITTGKSSMEVAKVISSYGAEVVALACIVDRSSETLTLPVYSAIKMEI
                     ETYEKDNCPLCKQNIPYVKPGTRKSIS"
     gene            1269..1916
                     /locus_tag="PLU28_06345"
     CDS             1269..1916
                     /locus_tag="PLU28_06345"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HOG34717.1"
                     /translation="MKFEKQILLVFFIILIGLSAVFSSGIISLEKHTEKVPSHFEKLI
                     VETETSRIFLKKAATSQLVFEHDERLLSHNIEVFGNTFKITESTKEFRIDLATLFGKY
                     ELSLDSMPKEIFLQSGDGSISLSGITAEKISILTQEGNIYVEECSAVEELNIKTEEGL
                     VYLSLGDIDDYTINFSSAEGSLIINGRRFSNTSKIYGNGKKIINFESQSSDLIII"
     gene            complement(2052..3452)
                     /gene="aglA"
                     /locus_tag="PLU28_06350"
     CDS             complement(2052..3452)
                     /gene="aglA"
                     /locus_tag="PLU28_06350"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032450 - maltose alpha-glucosidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase AglA"
                     /protein_id="HOG34718.1"
                     /translation="MSSVKIGIIGAGSAVFSLKLVSDLCKTKTLWGSTVTLMDIDQSR
                     LEGAFFLAKRFAKEVGADLNFEKTLDLDKAIIGSDFVINTAMVGGHSFLEKMREIGER
                     HGYYRGIDTQEFNMVSDYYTLTNNGQFNYFLEIAKKIEKYSPVAWLLQAANPVFEGTT
                     LINRYTKTKMVGFCHGHYAVDNFVKELGLDSSKVDWQVAGVNHGIWLNKFFYNGKDAY
                     NMIDTLKEKALKHKPLHPFDDQISPAAFDAYEFYGMMPIGDTVRNSSWKYHYDLDHKK
                     KWYGEPWAGADSEIGWKWYQNKLAEVSAVTIEVAKILEQNPELPFSKIIESAKDRLPA
                     DFFVEASNIFDPKKLSGEQHIPFIDAVKNGNSGRFIVNILNKGSIIKGISEDVAVEVP
                     ALVDAGGIKPEKLEPMLNDRILKWYLAPRIMRMEWAIDAFINKDPNMVLEILMRDRRT
                     KSYEQAKAVVEEIFKK"
     gene            3589..4395
                     /gene="pgeF"
                     /locus_tag="PLU28_06355"
     CDS             3589..4395
                     /gene="pgeF"
                     /locus_tag="PLU28_06355"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HOG34719.1"
                     /translation="MQFKKAEQGTITYLTIPQLEELGARLIFTTKKNGLSDISENALN
                     LGLNTGDPIEKIRQNYSTLCSNLGIDSSRIVFSDQVHGCDIKHIDLKDLPNEDFINTR
                     TLHAIDGISTNLSGIWLTTLYADCIPIFFYDFRQRAVAVSHAGWKGTALRIGEKTVDF
                     MQKNYGSRPCDILCAIGPGLCRKHFEVQKDVFNQLQKLPYSQNFCVYEREVYTVDLKL
                     ANQETLLNCGILKENIVCSELCTFEEEELFFSYRRDKGKTGRMAGMISIL"
     gene            complement(4406..5251)
                     /locus_tag="PLU28_06360"
     CDS             complement(4406..5251)
                     /locus_tag="PLU28_06360"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOG34720.1"
                     /translation="MNFKELKKKAADHIYSSLNYISAEDMGDVIQNFFVVCDKGVFII
                     SEEKKQYHVLWACDDFSFVVDNLKKFIDEYSDFQEGKRIFVEFIQPKDIVGLAKIGFK
                     IVSEWMDYWIEDINKIDIQKERFSQVFTISEDEYRTAAEVTLRCAGMSREYEGESYAF
                     VREWAKTRNSKVLVYKQDESICGVCFISIYRSSSAKANVLWVRELAVDPLYQGKGVAT
                     VLLNKALEYGKNWGAYKSFLAVDRENFRAVRIYEKIGFHPKAGHGQINMAYNVLNRVL
                     EKTDA"
     gene            complement(5376..5714)
                     /locus_tag="PLU28_06365"
     CDS             complement(5376..5714)
                     /locus_tag="PLU28_06365"
                     /inference="COORDINATES: protein motif:HMM:NF014119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOG34721.1"
                     /translation="MNSLGAVYEDCAVKFLKRKGFKILKRNFSVKSGEIDIICMDGNT
                     LVFVEVKGGENYLSSPAYRAGIGKLIKISKVGQIFISMNEGLKFEEVRIDVVAVDKGM
                     NVEYFPSQRF"
     gene            complement(5714..6013)
                     /gene="gatC"
                     /locus_tag="PLU28_06370"
     CDS             complement(5714..6013)
                     /gene="gatC"
                     /locus_tag="PLU28_06370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HOG34722.1"
                     /translation="MAIKVDDSLIKKLERLSKISLSKDEEESLKNDLNTILEYMSMLD
                     EADVSGVREMYSPASDELTTVLHDDLPEKFDGAQNIVGQFPKKRGDLLVVPSIQN"
     gene            complement(6026..6424)
                     /locus_tag="PLU28_06375"
     CDS             complement(6026..6424)
                     /locus_tag="PLU28_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34723.1"
                     /translation="MTEMKGSAVIQIDSETVRAFLEKIVEGNAGSFIKKIFDSSSKLR
                     FYDNKIELSVLFFKFYAKLVQFPSTLCGVYEFEHNIPAEKLNISKLPQNIKIKDNKLY
                     FFVPENVLTQNLIIRSMKISDDKIYIELTY"
     gene            complement(6417..6875)
                     /locus_tag="PLU28_06380"
     CDS             complement(6417..6875)
                     /locus_tag="PLU28_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34724.1"
                     /translation="MSGLYIRMDQDFVNGLVDKVIESNTKIPWLKNLNVQIKDEGMHF
                     QADIEFMGEEKRLDSLISIKNKPQSLSQGSLVLALSGEEGIVKILQGIFLLLSRFSGG
                     FNSRENNIEINLEKINADPFIKEVFSYMKLDKMDFKDGEILMELSYRESD"
     gene            7014..7703
                     /locus_tag="PLU28_06385"
     CDS             7014..7703
                     /locus_tag="PLU28_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis CcdA family protein"
                     /protein_id="HOG34725.1"
                     /translation="MENQLALSVTQSIDVLTALGGGFLSFFSPCALPLVPIFLGILLP
                     DISNFKSTLKRAGAFFLGLSVFFSILGTASGFLGYFLSQYQWIINIAGGIFIAVLGLL
                     YLFEVQLFKGKAINFNKFRNNSFLSAFLMGILISFVWLPCSGPVLGAILTLAAARSSV
                     WYGGLLLFVYSLGISLPFLFLGGLISKITSKISFGQPKWQKYLRLAGGIIMIAMGIVM
                     MLGLFNKLQIT"
BASE COUNT         2470 a         1599 c         1243 g         2416 t
ORIGIN      
        1 ccttgacagt tcaaaaatag atattataga actcaacata tcatgcccaa acgtcaaaga
       61 aggcggaatg aacttcggaa taaaaaccca aagcgccgta caagtactcg aacaggtaag
      121 aaaagccgtt tcaaaacctt tgattgtaaa actctctcct aatgctgagg atatagtaga
      181 tatggccgtt acctgcgaaa aaaatggtgc ggacggtatc tctcttgtaa atacttttaa
      241 agcaatggca attgatatat ataaaaggaa accgatattt aaaaatataa ctgcaggact
      301 ttcaggaccg tgcataaaac ccattgccct gcgtatggta tatgaagtaa gcaaagcagt
      361 taatattcct gttatcggta tgggaggaat aagctgctgg caggatgccg tagagtttat
      421 aatggctgga gcatgcgcag ttcaaatagg cactatgaac tttataaagc cagacatctg
      481 ccttgacata ataaatggaa tagaaatgtt tatggaaaaa aataacttta acagcatatc
      541 tgaaatgaaa tctataatat aggaggaaaa aatggatatc aatgaaatct taaaagaaac
      601 cggtgctctt ctccagggac attttcttct ttcatcagga aaacacagca acaaatacgt
      661 ccagtgtgcc aagctcatga tgtatcccga aaaaactcaa actgtactta atcaggttgc
      721 tcaaaaactt aaaaaccttg aattcgatat tatagtcggt cctgcaatcg gcggaatagt
      781 acccgcttat gaactggcaa gacttacagg aaaaatctgc atgtatgcag aaagagaaaa
      841 cgacgttatg actctcagga gaggctttga actctttccc ggtcaaaaag ttataatctg
      901 tgaagatgta attacaacag gaaaatcttc catggaagtt gccaaggtaa ttagctccta
      961 cggagcagaa gtagtcgctc ttgcctgcat cgtggacaga agcagcgaga cgcttaccct
     1021 tccagtatat tcggcaataa aaatggaaat agaaacttac gaaaaggata actgcccgtt
     1081 gtgcaaacaa aatatccctt atgtaaaacc gggcacccgc aagagcattt cataaaaatc
     1141 gaaaaacctt tttaggcctt ataatgtaca ggttgtaccc cctttttcat tttttatcat
     1201 aaataggttt gaatactaat tttaaaatta tagaataaaa gtaacacaac agaaaaaggg
     1261 ggatttatat gaaatttgaa aaacagattc ttctagtatt ctttattatt ttaattggac
     1321 tgtcagctgt tttttcatcg ggcattatta gtttggaaaa gcatacagaa aaagtacctt
     1381 ctcattttga aaaacttata gtagaaacag aaacatcaag gatttttctg aaaaaagctg
     1441 ctacttccca attggtcttt gaacatgatg aaaggcttct ttcccacaac attgaagttt
     1501 tcggaaacac cttcaaaata accgaatcta ctaaagagtt caggatagac cttgccacgc
     1561 ttttcggaaa gtatgagctt agccttgaca gcatgccaaa agagattttt ctgcagagcg
     1621 gcgatggcag cataagctta tccggtatca cagccgaaaa aatatcgata cttacacaag
     1681 aaggaaatat atatgtggaa gaatgttctg ctgttgaaga actaaatata aaaactgaag
     1741 aaggtcttgt atatttaagt ttgggagata tagacgacta caccataaat ttttcttctg
     1801 ctgagggtag cctcataata aatgggcgaa gattcagcaa tacatcaaaa atctacggaa
     1861 acggaaagaa gatcataaat tttgaaagtc aatcatcaga tctaataata atatagatta
     1921 ttctttaatc tttctaaccc aaaaatcccc tgtggaaaaa caggggattt tatttttact
     1981 ctttttgtca agctctttct taaagtcaaa aaaaagcctt ccaatgtagt aaccattgaa
     2041 aggcttttaa actatttttt aaatatctcc tcaacaactg ctttcgcctg ttcataagac
     2101 tttgttcttc tgtcacgcat aagtatttcc agcaccatat tcggatcttt attaataaaa
     2161 gcatcgattg cccattccat tctcattatt cttggtgcaa gataccattt taaaattctg
     2221 tcattaagca taggctcaag tttttccggt tttattcctc cggcatccac cagtgccgga
     2281 acctccacgg caacatcttc ggaaatacct tttatgatac tgcctttatt tagtatatta
     2341 actataaatc tcccggaatt accgttttta acagcatcaa taaacggaat atgctgttca
     2401 ccgctaagtt ttttcggatc aaaaatattt gatgcttcaa caaaaaagtc agcaggaagt
     2461 ctgtctttcg cggactctat tatttttgag aacggaagct caggattctg ttcaagtatc
     2521 ttagctacct ctattgtcac tgctgatact tcagcaagct tgttctgata ccacttccaa
     2581 cctatttccg agtctgcccc tgcccatggc tcgccatacc acttcttttt atgatccaga
     2641 tcataatgat acttccagct gctgtttctg acggtatctc ctataggcat catgccgtag
     2701 aactcataag cgtcaaatgc agcaggcgat atctgatcat caaaaggatg aagaggctta
     2761 tgttttaatg ctttctcttt aagagtatct atcatattat atgcatcttt tccgttataa
     2821 aaaaacttat taagccagat cccgtgattt actccggcta cctgccagtc tactttgcta
     2881 ctgtcaagcc caagctcttt aacaaaatta tccaccgcat aatgaccatg acaaaatccg
     2941 accattttgg tttttgtata tctgtttata agagtcgttc cctcaaaaac cggatttgca
     3001 gcctgtaaaa gccatgccac gggagaatac ttttctatct ttttggctat ttccagaaag
     3061 taattgaact gtccgttgtt tgtaagagta taataatctg aaaccatgtt aaactcctgc
     3121 gtatctattc ctctataata gccgtgtctt tcacctattt cacgcatttt ttccaagaat
     3181 gaatgaccgc ccaccattgc cgtgttaata acaaagtcag agcctattat tgccttatca
     3241 agatcaaggg ttttctcaaa gttcaaatct gctccgacct cttttgcaaa tctcttcgca
     3301 agaaaaaatg ctccttccag tctgctttgg tctatatcca tgagcgtaac cgtagaaccc
     3361 caaagagtct ttgttttaca aagatcgctc acgagcttta atgaaaaaac ggcacttcct
     3421 gcacctataa tacctatctt aaccgatgac ataataaatc cctcctttta ttaaccgtat
     3481 gtttcattaa aattatacat caaaaaatgc tttgctataa ttaaacaaaa tgatttctga
     3541 tttttattgt ataatattct ttataatccc aaaaaggaga gatacgatat gcagttcaaa
     3601 aaagcagagc aaggaactat tacttatctc actatcccac agttagaaga actgggtgca
     3661 cggcttatat ttaccaccaa aaaaaacggt ctctcagata tatcagaaaa cgctcttaat
     3721 ctcgggctca acaccggtga tcctattgaa aagataagac aaaattacag tactttatgt
     3781 agcaaccttg gcatagacag ttcaagaata gttttctctg accaggttca tggatgcgat
     3841 ataaagcata tcgatcttaa agacctcccg aacgaagatt ttataaatac aagaacctta
     3901 catgcaatcg acggaatatc taccaatctc agtggaatat ggcttacaac cttatacgct
     3961 gactgtattc caattttttt ttatgatttc cgtcaaaggg ctgtcgctgt ttcacatgcg
     4021 ggttggaaag gaacagctct cagaatagga gagaaaaccg ttgattttat gcaaaaaaat
     4081 tacggatcaa gaccatgcga cattttgtgc gcaataggcc ctggtttgtg ccgcaaacat
     4141 tttgaagtac agaaagatgt ttttaatcag ctgcaaaaac ttccttacag ccaaaatttt
     4201 tgtgtatacg aaagagaagt ttacactgtt gatctgaaac ttgctaacca ggaaacactt
     4261 ttgaactgcg gaattttgaa agaaaatatt gtttgttcag agttgtgcac ctttgaagaa
     4321 gaagagcttt tcttttctta tcgcagagat aagggaaaaa ccggacgaat ggccggcatg
     4381 ataagtattt tatgataacc gaaaatcatg catcagtttt ttccaatact ctatttaaga
     4441 cgttatacgc catattaatc tgaccatgac cggctttggg atgaaaccca attttttcat
     4501 agatccttac tgcacggaag ttttccctgt ctacggcaag aaagctttta taagcccccc
     4561 aattttttcc gtactcaaga gccttgttaa gcaaaacagt tgctactcct tttccctgat
     4621 acagaggatc gacagcaagc tctcttaccc aaagaacatt tgctttggca gaacttgatc
     4681 tgtatatgct tataaaacat actccgcaga tgctttcatc ctgcttgtac accagaactt
     4741 tagagtttct ggttttggcc cactctctta caaatgcata actctctccc tcatattcac
     4801 gtgacatgcc tgcacacctt aaagtgactt cagcagctgt acggtattca tcctcagaaa
     4861 ttgtgaatac ctgcgaaaac cgttccttct gtatatctat tttattaata tcctctatcc
     4921 agtaatccat ccattcacta actattttga accctatctt agcaagcccg acaatatctt
     4981 taggctgaat aaactctaca aaaatacgtt ttccctcctg aaaatcgctg tattcatcta
     5041 taaatttttt caaattgtct actacaaaag aaaaatcatc acatgcccat aaaacatgat
     5101 actgcttttt ttcttctgag ataatgaata ctcctttatc acaaaccaca aagaaattct
     5161 ggatcacatc tcccatatct tccgcagaaa tatagttaag cgagctgtat atgtggtctg
     5221 cagccttttt tttgagttcc ttaaaattca taataatccc tcctttctga ttatatatta
     5281 tcattttaaa aaaattataa aaagtgaata tagtcatttt tttattcata aaaaaaatat
     5341 cagccgccat tacggcggct aatataaaat ttattttaaa atctctgaga aggaaagtat
     5401 tctacattca tccctttatc tacagcaaca acatctattc taacctcttc aaacttcagt
     5461 ccctcattca tagaaataaa aatctgacct acttttgaaa ttttgatcag ttttccaata
     5521 cctgccctgt atgccggaga tgaaagataa ttttctcctc cctttacctc gacaaaaaca
     5581 agcgtatttc cgtccataca tataatatct atctcaccgg actttacgct gaaatttctt
     5641 ttcaaaattt taaaaccttt tctcttcaaa aacttaacag cacagtcctc atatactgct
     5701 cccaagctgt tcatcagttc tgaattgaag gaacaaccag aagatctccc cttttctttg
     5761 gaaactgccc gactatgttt tgggctccat caaacttctc aggaagatca tcatgcagaa
     5821 cagtcgttaa ttcatcgctt gccggagaat acatctccct gacacctgaa acatcagctt
     5881 catcaagcat actcatatac tcaagtatgg tgttcaaatc atttttaagc gattcctctt
     5941 cgtctttgga aagagaaatt tttgaaagcc tctcaagttt tttaataagc gaatcgtcta
     6001 cttttattgc cataaagtac ctccatcagt aagtaagttc tatataaatt ttatcatcac
     6061 ttattttcat agaccttata ataagatttt gcgtgagaac attctccgga acaaaaaaat
     6121 ataacttatt atcctttatc tttatatttt gaggcagctt tgaaatattg agcttttcgg
     6181 caggaatatt gtgctcaaac tcgtacactc cgcataaagt agaagggaac tgcacaagct
     6241 ttgcataaaa tttaaaaaac agaactgaaa gttctatctt attatcataa aatcttagct
     6301 ttgaagaaga atcaaaaatt tttttgataa aagaaccggc atttccctca acaatctttt
     6361 ccaaaaaagc tcttacagtc tcactatcaa tctgtatgac ggcagatcct ttcatttcag
     6421 tcactttccc tataactcaa ttccattaag atttcaccgt ctttgaaatc catcttatca
     6481 agtttcatat acgaaaaaac ttccttaata aaaggatcgg catttatctt ttcaaggtta
     6541 atctctatat tattctctct agagttaaat ccaccgctga atcttgagag taacagaaaa
     6601 ataccctgaa gtatctttac tataccttcc tctcccgaca aagccagaac cagactcccc
     6661 tgtgacaagg actgcggttt gttttttatt gatattagag aatcaagcct tttttcctct
     6721 cccataaact ctatatccgc ctgaaaatgc attccctcat ccttaatctg aacattaaga
     6781 ttcttaagcc aaggtatttt ggtattagac tctatcacct tatcaaccag accatttaca
     6841 aaatcctgat ccattctgat atataacccg gacataaagc acctcccgca tgaagtcgtt
     6901 gagattgagg cataattttt cttatttcaa ttatatcaca ataaaaaagt tgaaaaaatc
     6961 tgactttata tgttataatt aaaatacccg tatggggtaa aaaggaggta tctatggaaa
     7021 accaactggc actgtctgta actcaatcta tagatgtttt aactgctttg ggaggcggct
     7081 ttctgtcctt cttcagtccc tgtgcccttc cccttgttcc tatattctta ggaatacttc
     7141 tgccggacat ttcaaacttc aaaagcacac tcaaaagggc aggagcattt tttcttggac
     7201 tgtcggtatt tttttccatt ctcggaacag catcgggctt tctgggctac ttcctatcac
     7261 agtatcaatg gataataaat atagcaggag gaattttcat agcagttctt ggcttgcttt
     7321 acctttttga agttcagctt tttaaaggca aggccatcaa ctttaacaaa ttcagaaaca
     7381 actccttttt atcggctttt ctgatgggca tactcatctc ctttgtttgg ttaccgtgtt
     7441 caggaccagt tctgggagca attctgactc ttgccgctgc aagatcaagt gtctggtatg
     7501 gaggtctact tttgtttgta tactctcttg gaatttcctt accgtttctt ttcctcggag
     7561 ggcttataag taaaataact tccaaaattt ccttcggaca accgaaatgg cagaaatatc
     7621 tacgtcttgc aggcgggatt ataatgatag ctatgggaat agtaatgatg ctaggacttt
     7681 ttaacaaact acagataact tgaggtgata ctatgaaaaa aggcataa
//
LOCUS       DAOSHR010000095         7721 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_8174_length_7721_cov_6.297939, whole genome shotgun sequence.
ACCESSION   DAOSHR010000095 DAOSHR010000000
VERSION     DAOSHR010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 7721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7721
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8174_length_7721_cov_6.297939"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1607)
                     /gene="pruA"
                     /locus_tag="PLU28_06390"
     CDS             complement(<1..1607)
                     /gene="pruA"
                     /locus_tag="PLU28_06390"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HOG34726.1"
                     /translation="MDNGIISIQRPYNEPTFSYAPGSPERKAIIEKITELKNTLLEIP
                     LIIGGKEIKTGTLGECRMPHDHKHKLAVYHKAGAQEVKYAIEEALKAKKTWESINWEE
                     RAFIFQKAADLLSGPYRATVNAATMLCQSKNVFQAEIDSACELIDFFRFNAFYMQQIY
                     REQPFSAPGTFNRLEYRPLEGFIYAVTPFNFTSIGGNLPSAPAIMGNTVIWKPAQTAV
                     YSNYFIMKLFMEAGLPAGVINFVPGNSSMISEIVFSERDFAGVHFTGSTEVFNGMYRK
                     IGENIDKYRTYPRIVGETGGKDFIFVHESADVDSVTTALVRGAFEYQGQKCSAASRAY
                     IPQNLWEKIKIKLIQQVRDISTGSPEDFRNFVNAVIDKSSFDKIKEYIEYCKSNSDAE
                     IITGGKYNDSVGYFIEPTTVLVKDPFFKTMQEEIFGPLLTIYVYPDGKYEETLEICDR
                     TSLYGLTGSIFAKDRLAVLKAQSILKNSAGNFYINDKPTGAVVGQQPFGGARGSGTND
                     KAGSYLNLLRWTSPRTVKETFISPNDF"
     gene            complement(1642..2787)
                     /locus_tag="PLU28_06395"
     CDS             complement(1642..2787)
                     /locus_tag="PLU28_06395"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOG34727.1"
                     /translation="MKNKANVVVIGGGVVGCSIAYNLALQGISDVVLIEKSFLASGST
                     GRCGAGVRQQWGTKMNCLLAQKSMEILENMNDILHTKRDIELKQKGYLLLAYTEKEMN
                     QFKKNVELQNSLGIASKIITPAQAREIVPYLNTEKLLGGAFYEKDGHANPFLVTNAYA
                     QAAISLGAEVNTYTEVVSIKRNGSKIEGVQTSKGFISTDTVIDAAGGYSKEIGKMAGV
                     DIPVYPQRHQILVTDPVDEVLGPMVMSFSYNIYCQQSPQGSFIMGYGDPTEPRDYNIN
                     SSWRFLEEMAEKAVWLLPPLKNLRVVRQWSGLYTMTEDRQPLICKADEAENFYMACGF
                     SGHGFMIAPMVGKLISEMVIGKKLSIDIELNYKRFEKGELLFEPSVV"
     gene            complement(2777..3052)
                     /locus_tag="PLU28_06400"
     CDS             complement(2777..3052)
                     /locus_tag="PLU28_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HOG34728.1"
                     /translation="MENKTIICRCEDITVEEIRSIIKEGFTTLEEIKRITRCGMGPCQ
                     GKTCTPIIAREISRITGKSIDEIIPSKARPPVGGIALGELTGDKNEK"
     gene            complement(3042..3542)
                     /locus_tag="PLU28_06405"
     CDS             complement(3042..3542)
                     /locus_tag="PLU28_06405"
                     /inference="COORDINATES: protein motif:HMM:NF012267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOG34729.1"
                     /translation="MLKKNGVACKEEYMEKLPSDIRKEKGPFAMIECFQRIPCDPCVY
                     SCPIKAIMPMSDINDIPRVDFDKCNGCASCVVKCPGLAIFVVDLSKSSGKAVLKMAYE
                     LLPVPAVGDEVVALSREGKELCKAKVVEVRDGKINDKTYLLGIELDREYIYEARAVRV
                     VDSDGK"
     gene            complement(3535..4623)
                     /locus_tag="PLU28_06410"
     CDS             complement(3535..4623)
                     /locus_tag="PLU28_06410"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOG34730.1"
                     /translation="MIYTDLMIIGGGPAGLSAAKTVSDYGVKSILVEKDRKLGGQLVK
                     QTHRFFGSKKEKAGMRGFTIAQNIENEIRKSGSSEIWTDSTVLGLYEDGVVTVLRNGR
                     MEKIKPKKIIAATGAFERYLAFENNDLPGVFGAGAVQTLMNVFGVLPGKKVLMVGSGN
                     IGLIVSYQLIQAGAKVVQLVEAAPEIGGYLVHASKIARLGVPILTSATLKGVYGNGKV
                     EKAVIHRVDERFNPIDGTEEEIEVDTVCLAVGLTPLNELVRMAGCAMVYSGELGGYVP
                     LRDDFMQTSVNGLYCCGDASGIEEATAAMLSGSIAALHACYSIFPDIQDYQEQMQRLQ
                     SALTELRAGPFGEKIRKGLSRAVVIKNA"
     gene            complement(4620..4925)
                     /locus_tag="PLU28_06415"
     CDS             complement(4620..4925)
                     /locus_tag="PLU28_06415"
                     /inference="COORDINATES: protein motif:HMM:NF024900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HOG34731.1"
                     /translation="MRIENHPVLEYKRGKKITFFFNGMPVECFEGETIAAALYAANIL
                     DLSSSRRLHRPRGLFCAIGNCSSCLMEVDGVVNVKTCVTPARHGMHVCVQEGKGDLL"
     gene            5041..5130
                     /locus_tag="PLU28_06420"
                     /pseudo
     CDS             5041..5130
                     /locus_tag="PLU28_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611142.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(5196..6527)
                     /locus_tag="PLU28_06425"
     CDS             complement(5196..6527)
                     /locus_tag="PLU28_06425"
                     /inference="COORDINATES: protein motif:HMM:NF015185.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG34732.1"
                     /translation="MAEKSKRTGMWSLLLMVATLIILNADQMVMSPSINAIEKDFGVT
                     DREIGFITSAFTVIGALISLVWGYFGDKYNRKKLLFFSVLVGEIPCLLSAFSMNFTML
                     FIFRALTGIGVGASFPLVFSMAGDMFEEKSRPKAMAIISTSIAIGQIVGMAVAGFTNT
                     ITPLTWRLPFLLVSIPNFIIIIIFSLFVEEPKRGSKEYAIGELVESGIDYKKKISAGD
                     YLNLVKIKTNFLLFIQGVLGTIPWGAIPYFLVTFFEREKNIPPSMGTIIFLIFGVGNL
                     FGILTGGYIGNVLYNIRKGWVSLFCGITTALGAFLAMATLNFNYSDEKFYLLSILGFF
                     TAFTLSLTGPNVKTLLINVNKPEDRARIFSVFNLTDSLGTGIGQLFAGSISAGLALKG
                     ISNSLGKALNYSALFWFGCAVLLCLLALFVSKDVQKLKNYFSNLKIQMLKK"
     gene            complement(6591..7097)
                     /locus_tag="PLU28_06430"
     CDS             complement(6591..7097)
                     /locus_tag="PLU28_06430"
                     /inference="COORDINATES: protein motif:HMM:NF014669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HOG34733.1"
                     /translation="MNYKSISKIGMFVAIMAVFSWINIPLPIGIPFSMQVLGVFLTSF
                     FLGANAWKVFLTYLLLGIIGIPVFSGFRSGIEVIIGPTGGFLIGFLVSSFLGRFERVH
                     KLVLGFLQILTIYSCGIIWFMIYKNVSFVFAFQILVPSFIWIDSLKLIAAYVIYKAVN
                     SRAIKRLS"
     gene            complement(7164..>7721)
                     /locus_tag="PLU28_06435"
     CDS             complement(7164..>7721)
                     /locus_tag="PLU28_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34734.1"
                     /translation="IIIVLSLNSKLEWGLFFISLDFLSERVLSLFLFLLIPSFMCYKS
                     FVKSKNNSIFKFLLFGYLSFVITSLLHILFVFIGTNVSITAEKWGMIIFFACCLSILV
                     VHHIRSARSTVESYKKVLYQNNELKSKMKKTQQNTDESLNKVIKHMNELNSFLDEGIL
                     EKKEHEVKKIIKKYSELISSFISKV"
BASE COUNT         2381 a         1733 c         1176 g         2431 t
ORIGIN      
        1 ttaaagtcat ttggcgaaat aaatgtttct tttaccgttc gaggggatgt ccagcgtagt
       61 agattcagat aacttccggc cttatcgttt gtccccgagc ctctggctcc tccaaaaggc
      121 tgctgaccaa caactgcccc tgtaggttta tcatttatgt aaaaattccc cgctgagttt
      181 ttaagtatac tttgcgcctt cagaacagca agcctgtctt tggcaaatat tgaccctgta
      241 agcccatata aagacgtcct gtcgcaaatc tccagagtct cttcgtactt tccgtcagga
      301 tacacataaa ttgtaagcaa ggggccaaaa atctcctcct gcatagtttt aaaaaaagga
      361 tcctttacaa gcactgttgt gggttcaata aagtatccta cagaatcatt gtactttcca
      421 ccggtaatta tttcagcatc agaattactt ttacagtact ctatgtactc ctttatctta
      481 tcaaacgaac ttttatcaat aactgcattg acaaagtttc tgaaatcctc aggactgcct
      541 gtgcttatgt cgcgcacctg ctggataagt tttatcttta ttttttccca taggttctgc
      601 ggtatatatg cgcgtgatgc tgcagaacac ttctgcccct gatactcaaa agctcctctt
      661 accaatgctg ttgtcactga atcgacatca gcagattcat gaacaaaaat aaagtctttt
      721 cctccggttt cacctacaat tcgcggataa gttctgtatt tatctatatt ttcacctatt
      781 tttctataca ttccattaaa aacctctgtg gaacctgtaa aatgaactcc agcaaaatca
      841 cgttcagaaa aaacaatttc agagatcata gaagaatttc ctggcacaaa atttattact
      901 cctgcaggaa gacctgcctc cataaacagt ttcattataa agtaattcga ataaactgct
      961 gtctgcgctg gtttccatat cacggtatta cccatgatag caggagcaga aggtagattt
     1021 ccgcctatgg atgtgaaatt aaaaggagtt accgcatata tgaatccttc taatggtctg
     1081 tattcaagcc tgttaaaagt accgggagca gaaaaaggct gttctctgta tatctgctgc
     1141 atataaaaag cattaaatct gaaaaaatct ataagttcac atgctgaatc aatctctgcc
     1201 tggaaaacat ttttgctctg gcaaagcatt gtagccgcat tcactgtagc tctgtatggt
     1261 ccagaaagaa gatctgcagc cttttgaaat ataaatgctc tttcttccca gttaatggat
     1321 tcccatgttt ttttagcttt caaagcttct tctatcgcat attttacttc ctgtgcaccg
     1381 gccttgtggt atactgcaag tttgtgttta tggtcatgag gcatacggca ttccccaaga
     1441 gttccagttt ttatttcctt tccgcctatt ataagaggta tttccagtaa tgtatttttt
     1501 agttcggtta ttttctcaat tatggctttt ctttcaggac tgccaggtgc ataggaaaaa
     1561 gtaggctcat tataaggcct ttgaatagat atgataccat tgtccattaa tatcccctcc
     1621 atcaactttt atacattcaa attatactac cgaaggctca aacaacagtt cgcctttttc
     1681 aaaccgttta taattaagtt caatatcaat actcagcttt ttaccaataa ccatttctga
     1741 tatcaattta ccaaccatag gtgcaatcat aaatccatgt ccgctgaacc cacatgccat
     1801 gtaaaaattt tcagcttcat cggctttaca tatcaacggc tgtctgtctt cagtcatggt
     1861 ataaagacca gaccattgtc ttaccacacg aagatttttc aacggaggaa gcaaccatac
     1921 cgccttttca gccatttctt caagaaatct ccatgacgag tttatgttat agtctctggg
     1981 ttcggtagga tcaccgtacc ccattataaa acttccttgc ggagattgct gacagtagat
     2041 attatatgaa aaagacataa ccataggacc aagaacttca tcaaccggat ctgtaacaag
     2101 tatttgatga cgctgaggat atacaggaat atcaactcca gccatcttac ctatttcttt
     2161 agaatacccg cctgctgcgt ctattacagt atctgtactg ataaatcctt tacttgtctg
     2221 aactccttct attttgctgc cgtttctttt aatcgacaca acctcggtat atgtattaac
     2281 ctcagctcct agagagatag ccgcctgcgc ataagcattt gttaccagaa aagggtttgc
     2341 atgcccatct ttttcataga aagcaccacc caaaagtttt tccgtattca gataaggcac
     2401 tatttctctc gcctgtgcgg gagtaattat ttttgaagct attccaagag aattctgaag
     2461 ttcaacattc tttttaaact gattcatttc tttttctgta tacgcaagaa gaaggtagcc
     2521 tttctgcttg agttctatat ctcttttcgt atgaagaata tcgttcatgt tttctagtat
     2581 ctccatactt ttctgtgcca gcagacagtt catttttgtt ccccactgtt gcctgactcc
     2641 tgctccgcac ctcccagtag atccgcttgc aagaaaactt ttttcaataa gtactacatc
     2701 gctgatgcct tgtaatgcaa gattataggc tatagaacaa cctacgaccc ctcctcctat
     2761 aacaactaca ttcgctttat ttttcattct tatcacctgt taattcaccc aaagctattc
     2821 ctccaaccgg tggacgagcc tttgatggga taatctcatc aatacttttt cctgtaattc
     2881 gcgatatttc ccgagctatt ataggagtgc atgttttccc ctgacatggt cccattccac
     2941 atcttgttat tctttttatt tcttcaagag ttgtaaaacc ttctttgata atactacgga
     3001 tctcctcaac tgtaatatct tcacaccggc atattatagt tttattttcc atctgaatca
     3061 accaccctta cggctctggc ctcatagata tactcgcggt caagctctat tcctaaaaga
     3121 taggttttat cgttaatctt cccgtctcta acttcaacta cttttgcttt acaaagttct
     3181 ttcccttctc tggaaagagc aacaacctca tccccgaccg ccggaacagg aagaagttca
     3241 tatgccattt tcaatacggc ttttccagac gactttgaca gatcaaccac aaatattgcc
     3301 agtccgggac atttgactac acatgaagca cacccgttac acttatcaaa atcaaccctc
     3361 ggaatatcgt ttatatcaga cataggcatt attgctttta ttgggcaact ataaacgcaa
     3421 gggtcacatg gaattctctg aaagcattct atcatggcaa aaggtccttt ttcttttctt
     3481 atatcggatg gaagtttttc catgtactct tctttacaag ctactccgtt ttttttaagc
     3541 attttttatc accacagctc tggataatcc ttttcttatt ttctcaccaa aaggtcctgc
     3601 tctaagctca gttaatgctg actgaagcct ttgcatctgt tcctggtaat cctgtatatc
     3661 cggaaaaata ctgtaacagg catgaagtgc agcgatactt ccgcttagca ttgctgccgt
     3721 tgcctcttct attccacttg catcaccaca gcagtacagt ccgtttacag atgtctgcat
     3781 aaaatcatct cttagcggaa catagcctcc aagttctcca gaataaacca ttgcacatcc
     3841 tgccatcctg accaactcat ttaaaggagt taacccaacg gctaaacata cggtgtcaac
     3901 ttcgatttcc tcttctgttc cgtctatagg attaaatctt tcgtccactc tatgaatgac
     3961 agccttttct accttaccgt tgccatagac tcccttcaaa gttgcactag tcaaaatcgg
     4021 aacacctagc cttgcaattt tggaagcatg tacaagatat ccgcctattt caggcgccgc
     4081 ttcaacaagt tgaactactt ttgcaccggc ctgtataagc tgatacgata ctataagacc
     4141 aatattgcca gatcctacca ttaaaacctt tttccccggt aaaactccga aaacattcat
     4201 aagcgtctga actgcacccg ctccgaatac acctggcaaa tcattgtttt caaatgccaa
     4261 gtatctttca aatgcaccgg ttgcagcaat aatcttttta ggctttatct tttccatcct
     4321 tccgtttctc aaaacggtaa ccaccccatc ttcatacaaa ccaagcactg ttgaatcagt
     4381 ccaaatctca gaacttccgg atttccttat ttcattctca atattctgcg caatcgtaaa
     4441 tccacgcata ccggcttttt ctttttttga accgaaaaaa cgatgagtct gttttacaag
     4501 ttgtcctcca agctttctat ctttctcaac taaaatactc ttcactccat aatctgatac
     4561 agtttttgca gcactcagcc ccgcaggacc tccgccgata atcataagat cagtgtaaat
     4621 cataaaagat cacctttgcc ttcctgaaca caaacatgca ttccatgtct ggcgggagta
     4681 acgcaggttt tgacattaac aactccatcg acctccatta gacatgaaga acagttacct
     4741 atggcacaaa ataatccacg tggtctatgg agccttcgac tactggaaag atctagaata
     4801 ttcgcagcat aaagagctgc tgctatagtc tctccttcaa aacattcaac aggcataccg
     4861 ttgaaaaaaa aggttatttt ttttccccgt ttatactcta aaactggatg attctctatc
     4921 ctcacactta tctcccccat ctttgtaaac agtaaaaacc ttttcgttaa aattatagca
     4981 tgaatatgaa aaaaatttca acatagttaa tatttgtaca aacaatagaa cagtaaaaaa
     5041 cattacaact acaatcatca taggatcaag ggcaagttaa aaggactgag ccctgttcaa
     5101 tacagaactc agtccttgtt aatagcttaa tgtactacca aatgtgtcta actttttggg
     5161 ttcagttcaa taatagccgc ttatcaattg aatcattatt ttttcagcat ctgtattttg
     5221 agattggaaa aataattctt aagcttctga acgtcctttg aaacaaataa agcaagcaga
     5281 caaagtaata ctgcacaccc gaaccagaat aatgctgaat agttcagagc ttttcccaaa
     5341 ctattggata tcccctttag tgcaagtcca gcagaaatac ttccggcaaa aagctgtcct
     5401 attcctgttc ccaatgaatc cgtcaaattg aaaacagaaa agattcttgc cctgtcttcc
     5461 ggtttattaa cgtttataag caaggttttg acgttcggtc ctgtaagcga taatgtaaaa
     5521 gcagtgaaaa aaccaagaat gctgagcaaa taaaactttt cgtcagagta gttaaagtta
     5581 agggttgcca tagccaaaaa agcgccaagt gctgtagtta ttccacagaa taaagaaacc
     5641 caacctttgc ggatattata gagaacattg cctatatagc caccggtaag tattccaaaa
     5701 aggtttccga ctccaaaaat cagaaatatt atggttccca ttgaaggagg tatatttttt
     5761 tctctctcaa aaaaagtaac cagaaagtat ggaatggctc cccaaggtat tgttcctaat
     5821 actccctgta tgaaaagtaa aaaatttgtt tttatcttta ccaaattaag atagtctcct
     5881 gcagatattt tcttcttata atcaatgcca gactctacaa gttctcctat tgcatactcc
     5941 tttgaccctc ttttgggctc ctcaacaaac aaagaaaaaa taattataat aatgaagttt
     6001 ggtattgaaa ccagaagaaa tggaagcctc catgtcagag gtgtaatcgt gttggtaaag
     6061 cctgcgactg ccattccgac aatctgtcct attgctattg aggttgatat tatggccata
     6121 gctttgggtc tggatttttc ttcgaacata tcccctgcca tagagaacac tagaggaaat
     6181 gacgctccta ctccaattcc cgtaagtgcc ctgaatatga aaagcattgt gaaattcatt
     6241 gaaaatgcag aaagaagaca cggtatctcc ccaaccagaa cactgaaaaa aagaagcttt
     6301 ttcctgttat acttatcacc aaaataaccc catacaagac tgatcagtgc ccctataact
     6361 gtaaatgctg aggttataaa ccctatctct ctgtcagtaa ccccaaaatc tttttcaatg
     6421 gcattgatac tcggtgacat aaccatctga tcagcgttaa gaattatcaa agtagccacc
     6481 ataagcaaaa gcgaccacat tccggttctt tttgactttt ctgccatttt tatcccccct
     6541 gaaataaaat ttatctatat tctatcataa attcattcat aggtataact tcaacttagt
     6601 ctttttatag ctcgactgtt aactgccttg taaatgacat aagcagcaat cagctttaag
     6661 ctgtcaatcc atatgaatga tggaacaaga atctgaaacg caaagacaaa ggaaacattc
     6721 ttatatatca taaaccatat tattccgcaa gaatatatcg tcaatatctg taaaaaacca
     6781 agtaccagct tatgaaccct ttcaaatctc cctaaaaaag acgaaaccaa aaaacctatc
     6841 aaaaatcctc ccgttggacc tataattact tcaataccgc tacggaatcc tgaaaaaact
     6901 ggaataccta taattcccaa aagcaaataa gtcagaaaaa ccttccacgc attagcccca
     6961 agaaaaaatg aagtaagaaa aactcctagc acctgcattg aaaaaggtat tccaattggc
     7021 aaaggaatgt ttatccatga aaatactgcc ataattgcta cgaacatacc aattttacta
     7081 atactcttat aattcatatt atcacctcgt ttgtatgata atacgaaagt atttattaaa
     7141 agttacaaaa gtattatgta tatttacacc ttgcttataa atgatgaaat aagttctgaa
     7201 tattttttta tgatcttttt tacttcatgc tctttttttt caagaatacc ttcatctaaa
     7261 aaactattta actcattcat atgtttaatt actttgttca gactctcatc tgtattttgc
     7321 tgtgtttttt tcattttgct tttcagttca ttattctggt ataaaacttt tttatatgat
     7381 tctactgtac ttcttgctga tcttatatga tgaacaacaa gaatacttaa gcaacaggca
     7441 aagaaaatta tcattcccca tttttctgca gttatgctga catttgtgcc aatgaaaaca
     7501 aaaagtatat gaagcaatga tgtaatgaca aaagataggt atccaaaaag caaaaacttg
     7561 aaaatactgt tattctttga cttaacaaat gatttataac acataaaaga tggaattagc
     7621 aaaaacaaaa ataaactcaa cactctttca gacaaaaaat caagactaat aaaaaacaaa
     7681 ccccactcca gttttgagtt aagggaaagc acaataataa t
//
LOCUS       DAOSHR010000096         7701 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_8190_length_7701_cov_5.370521, whole genome shotgun sequence.
ACCESSION   DAOSHR010000096 DAOSHR010000000
VERSION     DAOSHR010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 7701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7701
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8190_length_7701_cov_5.370521"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1072
                     /locus_tag="PLU28_06440"
     CDS             <1..1072
                     /locus_tag="PLU28_06440"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HOG34735.1"
                     /translation="ASLILWSYHMLIVSGVRTAGITNAVITIIKVLPLFVVLIVAITY
                     FKPDLFSNMFSTTIKTLEDGQVVARESSIWNQINSSFNTMLWTFIGIEGAVVLSNKAK
                     SQKDVAKVTLLGFLITISIYVLVSTLTMGVVPVEEILTSNSPLGTVLNNVIGKSGQYL
                     LDFGFLFSVMGALLSWLLLAAEIPYIAASRDHVFPKSFAKQNKKVTPVFSLLITNLIT
                     QAVLILLFTMSETGRIDNSENAILQNIYFAVISLAVICILIPYVSSAFLGIKTAYKEK
                     KYSNIIFSVLGIIFCIWVFTAMWKYATAAVIIYTSGIIVRYIAKKEKNQKFTRVEIAT
                     YLVMLVLSIISIYFVANGSIVY"
     gene            complement(1306..2268)
                     /gene="galE"
                     /locus_tag="PLU28_06445"
     CDS             complement(1306..2268)
                     /gene="galE"
                     /locus_tag="PLU28_06445"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HOG34736.1"
                     /translation="MILVCGGAGYIGSHLVKVLKESKKEVLVFDNFETGHEWAVKDTK
                     TFRGDLRELKDIQGVFKENSIDEVYHFAAYSLVGVSMQKPYEYFDNNIKGTLNLLKAM
                     TEHNVKNIVFSSTAAVYGDPKTLPITEKQEKIPTNVYGQSKLMIEQIMNWYSKLGMIN
                     YAALRYFNAAGASLDAQIGEDHTPETHLIPLILQTVLGKREKITVFGNDYPTADGSAV
                     RDYVHVLDLADAHIKAMKWIKENNRSDAFNLGNGEGFSVMQVIQKTQEVTGCNVKYEI
                     GARRSGDPAVLIASSEKAHTVLGWTPRYSRLEEIIQTAYNWHKK"
     gene            complement(2277..3203)
                     /gene="era"
                     /locus_tag="PLU28_06450"
     CDS             complement(2277..3203)
                     /gene="era"
                     /locus_tag="PLU28_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOG34737.1"
                     /translation="MENPLFKSGFIAVCGKPNVGKSSIINAIIGQKIVITSDKPQTTR
                     NKINCIYTQPHCQMVFVDTPGIHKPLHKFGEYLVKIAVNAVKGTDAILFVVDACEGIR
                     ASDEIVADIVRRSGVKAVLAINKMDIAKTENVAQEMENKIRSICPNITDTVNVSAKSG
                     KNISTLVQKLENMMPQGPMYYPQDEVTDKPSRFIVSELIREKILYLTQQEVPHSSAVV
                     VEDFEQRENGTLYIRANIYLERDSQKGIVIGKDGAMIKQIGQLARKDIERMFEEKVFL
                     DLFVKVKDKWRESDFLIMNTMGFKDEFKKGEK"
     gene            complement(3213..4313)
                     /locus_tag="PLU28_06455"
     CDS             complement(3213..4313)
                     /locus_tag="PLU28_06455"
                     /inference="COORDINATES: protein motif:HMM:TIGR03491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM0106 family RecB-like putative nuclease"
                     /protein_id="HOG34738.1"
                     /translation="MDILYYESLENCPFFTPSYTRPGSYFTINIEGVEFEANYEYYKD
                     GTVCIIRAGKSVRISHWIHAYLVYRYFEEKGNRVNDVLVELENRKFTIKRIELMNKEN
                     WIKKALARLNTRPKSAGAHCKFCTLKTACHRELLSQGDLNVVPAMTEGLIEHFKNIET
                     DPLEVVKSNQIEKFNPSCRKALYNLKSIIEDKIIKINDIVLPEKYIIFDVETYAERDF
                     LFGYLLEDRYIPYVFNSADSKKTEEMIDFLYNLKLPLVHYDVHDVQSLENIGRNYPAL
                     RTKISRILENSLDVFEVIIKNVSFPVTSYSLKDISKYLGFKWRTNLNGHAVIIEYNMM
                     LKGNKEILQKILSYNEDDCRATKVLMEKLREL"
     gene            complement(4332..5294)
                     /locus_tag="PLU28_06460"
     CDS             complement(4332..5294)
                     /locus_tag="PLU28_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34739.1"
                     /translation="MPLADIFENINIPDSIVSFVYGEDTKLFSYPSIVFFKGLSRENG
                     KGYAILIDFNDVMWIWESDNESSNDERVDALKKSVCSFTGFEYTPKKIDLEMEWEDFC
                     NHIDFFLQGQEIKILFEKFERSIRINEKELFSFNDSLENIKIISESLVDYMIRKDNLF
                     RIDIMNSAKTLLVSADLSIYADKVKSFETEFEKKIMWLAIDCLLKKNVLVIFDYLNPK
                     LVSKGVMAISIENVDPERFKDSITRIKESLILAGLDIDRVYNGLKEFMPYFNQMFELQ
                     KRVFNDKNYNVMDFAETVIIYYLFNNSNAICANVAQVKRIFGEL"
     gene            complement(5313..7352)
                     /gene="glyS"
                     /locus_tag="PLU28_06465"
     CDS             complement(5313..7352)
                     /gene="glyS"
                     /locus_tag="PLU28_06465"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HOG34740.1"
                     /translation="MNKLLFEIGVEELPSSEYSNIELQLEEIIRSELKTRNIEFSDIE
                     TVVAPRRFGAVVCGLSDKEPDTFTEKKGPARNLCFAEDGSPSRAFEGFLKSSGATVDQ
                     VIFKEINGIEYAHVKIDKKGKKTKELFEQILGPAITKMNFKKPMRWGKGEYSFVRPVH
                     WVLALYNDEIMNIEIFGKKSSNKTYGHRFFGREEIINNADEYFSKLRSSFVIARLSER
                     VEKISTELSRIEKESGYTCDRDKELIDEIAKLTEYPTAVVGSFDEKYSYLPPEITTVT
                     IKHHQRSFLALKDGKATNKYISFQDGYSREKNVVTGYSRVINARLDDAAFYYNDDLKA
                     VVQERFEQLSGVTYQQGLGTYKDKAERISAICAIIAERMGFKGNEVEKIKRAGLLCKI
                     DITSNVVFEFPEVVGSMGRIFLKAKNEEEDICTAAQEHYQPKDESDRTSSNITANIVS
                     IADKADDIAGYFGINKIPSGSKDPFALRRKAFGIIRILIDNEWDFDIKDILKIASDNL
                     NMKYDCEKMDEFFKSRFETILLKEEMPRDVINAVLVNWNRPLRAYLAAQSIKEYLSLD
                     KFKDFITAFQRIYNISKSHNSLQYQGRLFILEQEKELFQKYLEVRSGYESCVKEMNFK
                     GVIDILIEMKPQIDNYFEGVFVMDRDESIRLNRLGFLKTLSGMFSEIGDVSRLYQ"
     gene            complement(7345..>7701)
                     /locus_tag="PLU28_06470"
     CDS             complement(7345..>7701)
                     /locus_tag="PLU28_06470"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HOG34741.1"
                     /translation="QGIENVYNTKWNDTVDYGYLYKENERQFSVFNFEQSDTELLNKL
                     YGLYKNEFERLIEKGLYLPAYDFLAKTAHSFNLLDAAGAISVNERQKYILDIRSMSRK
                     CASTYVKAVNGGNSNE"
BASE COUNT         2118 a         1644 c         1278 g         2661 t
ORIGIN      
        1 tgcaagtctt attctctggt cttaccatat gctcattgta tccggagtaa gaacggcagg
       61 aataactaac gccgtaatta caatcatcaa agttcttccg ttgtttgtcg ttcttatagt
      121 cgcaataacg tactttaaac cagacctgtt ttcaaacatg ttctctacta ctataaaaac
      181 tcttgaagac ggacaggttg tcgcacggga gtcttctata tggaatcaga taaactcaag
      241 tttcaatact atgctctgga cttttatagg tatagagggt gccgtggttc tttccaataa
      301 agctaaatcc cagaaagatg ttgcaaaagt tactcttttg ggttttctga taactataag
      361 tatctatgta cttgtatcta ctcttacaat gggtgtagtg ccggtggaag aaatacttac
      421 atccaactcc ccgttgggaa ccgttcttaa caatgtcata ggaaaatccg gacagtacct
      481 tctggacttc ggtttcttat tttcggtgat gggagccctt ttgagctggc ttttactggc
      541 agcagaaatt ccttatattg ccgcatcaag agaccatgtt tttccaaaaa gctttgcaaa
      601 acagaataaa aaagtcactc cggtattttc tcttttaata actaacttaa ttactcaggc
      661 cgtgcttata cttctgttca caatgtctga aaccggaagg attgacaaca gtgaaaatgc
      721 aatacttcag aacatttact ttgcagttat aagccttgcc gtaatctgta ttcttattcc
      781 atatgtttca tcagcttttc ttggaataaa gacggcttat aaagaaaaaa agtattcaaa
      841 tataattttc tcggtacttg gaataatctt ctgcatatgg gttttcacgg caatgtggaa
      901 gtatgcaaca gctgcagtaa taatatatac ttcaggcata atagtgcgat acattgccaa
      961 aaaagaaaag aatcagaagt tcacccgcgt tgaaatagca acttatctgg taatgctggt
     1021 attaagtatt atatctattt atttcgtagc taacggaagc atagtatact gaaaagcctc
     1081 ccttacggga ggttttttca taaaaaaaaa ccgccctgtt ttagggcgat aaaggttttt
     1141 cgttctctat tggggggttc tgggagttct tacttctctt aaggaggttt agggtatctt
     1201 tctcacttgc atcataattc tataatacca tacttaaaaa atccttaaaa actttaatta
     1261 ttccgttatt cattgatatg gctttaaacc cctgtcccaa taaagttatt ttttatgcca
     1321 gttgtaggca gtctgaatta tctcttcaag tctactgtac cttggagtcc atcctaagac
     1381 agtgtgtgct ttttccgacg aggcaataag cactgcagga tctccgcttc ttcgtgcacc
     1441 tatctcatac tttacattac atccggttac ttcctgtgtc ttttgaataa cctgcattac
     1501 agaaaatccc tctccgttac ccaggttgaa agcatctgaa cggttattct cttttatcca
     1561 tttcatagct tttatatggg catccgcaag atcaagaacg tgtacgtaat ctctgaccgc
     1621 acttccgtca gcagtaggat aatcgtttcc gaaaacagtt attttctctc tttttcctaa
     1681 tacggtctga agtatcagag gtataagatg tgtttcaggt gtatggtctt ctcctatctg
     1741 tgcatccagg cttgctccgg cagcattgaa gtatctcagt gcagcataat ttatcattcc
     1801 gagttttgaa taccagttca taatctgttc gatcataagc ttgctctgtc cgtaaacgtt
     1861 ggtagggatt ttttcctgtt tctccgttat tggaagtgtt ttggggtctc cgtatactgc
     1921 cgcggtagat gagaaaacta tgtttttgac attatgctcc gtcattgcct taagcaggtt
     1981 cagagtccct ttgatgttat tgtcaaagta ctcatagggt ttttgcattg aaactcctac
     2041 aagcgagtaa gcagcaaagt gataaacttc atctatactg ttttctttaa aaactccctg
     2101 tatatctttg agttcgcgca gatcacctct gaatgtcttt gtatctttta cagcccattc
     2161 gtgaccggtt tcaaagttat cgaaaaccaa aacctctttt ttactttctt taagcacctt
     2221 tacaaggtgg gaacctatgt atccggctcc gccgcataca agtatcactc taccacctac
     2281 ttttcacctt ttttaaactc atccttgaag cccattgtgt tcataataag aaaatcgctt
     2341 tcacgccatt tgtccttcac tttcacaaac agatcaagaa aaactttttc ttcaaacatt
     2401 ctttctatat ctttgcgggc cagctgacct atctgcttta tcatggctcc gtccttgcct
     2461 atgactatac ctttctgcga atctctttcc agatatatgt tagctcttat ataaagagta
     2521 ccgttctccc tctgctcaaa atcctcaaca accacggcac ttgaatgtgg tacctcctgc
     2581 tgcgtaaggt agagtatctt ttctcttatc agttcagaga ctataaaccg cgatggctta
     2641 tcagtaactt catcctgtgg atagtacata ggtccctgag gcatcatatt ttcaagtttt
     2701 tgtacaagag tacttatatt ttttccgctt ttggcggaaa cattgaccgt atctgttatg
     2761 ttagggcaga tggatcttat tttattctcc atttcctgag caacgttctc agttttggct
     2821 atatccattt tattgattgc aagaactgct tttacaccgg accgtctgac tatatccgca
     2881 actatctcat cggaagccct tataccctca caggcatcaa caacaaagag tatggcatct
     2941 gttcctttaa cagcattcac ggctatcttt acaagatact ccccaaactt gtgcaaaggt
     3001 ttgtgaatac ctggcgtatc tacaaatacc atttggcagt ggggctgggt atatatgcag
     3061 ttaatcttgt tccgtgtggt ctggggttta tctgaggtaa ttacaatttt ctgacctatt
     3121 atggcgttta ttatacttga cttaccgacg tttggttttc cgcatactgc aataaaacca
     3181 cttttaaaca aaggattttc cattttgacc tcctacagct cgcggagctt ttccataagg
     3241 acttttgtag ctctgcagtc gtcttcgtta taactaagta ttttctgcag tatctcttta
     3301 tttcctttaa gcatcatatt atattctata ataacagcgt gtccgttaag attggttcgc
     3361 catttaaagc ccaggtactt gcttatatct ttcagtgaat atgaggtcac cggaaaagat
     3421 acattcttga ttataacttc aaacacatcg agagagtttt ccagtatccg tgaaatcttt
     3481 gtcctcagtg cggggtagtt tcttcctatg ttttccaggg actgtacatc atgaacatca
     3541 taatgtacca gaggcagctt gaggttatac aggaaatcaa tcatctcttc tgtttttttt
     3601 gaatctgcac tattgaaaac ataaggtata tacctatcct caaggagata gccaaaaagg
     3661 aagtctcttt cagcataagt ttcaacatca aatattatat atttttcagg cagtactata
     3721 tcgtttattt ttattatttt gtcttcaatg atggatttga ggttatataa agcctttcgg
     3781 cacgaaggat tgaacttttc tatctgattg ctttttacca cttcaagcgg atcagtttct
     3841 atgttcttaa aatgttctat aagtccttcc gtcatagccg gtacaacatt cagatcgccc
     3901 tgtgaaagca gttctctgtg acatgctgtt ttaagggtac agaacttgca gtgtgcacct
     3961 gcggatttgg gccgtgtatt cagccttgca agtgctttct ttatccagtt ttctttattc
     4021 attagttcta tgcgttttat ggtaaacttt ctgttttcaa gctctacaag aacatcatta
     4081 accctgtttc ctttttcttc aaagtacctg taaaccagat aagcatgaat ccaatgagat
     4141 atcctcacac tttttcccgc tcttattata cagacagtac catccttgta atactcatag
     4201 ttagcctcaa attcaactcc ttcaatattt atggtaaaat aacttcccgg cctggtataa
     4261 ctgggcgtaa aaaacgggca gttttcaagg ctttcatagt acagaatatc cataataaca
     4321 cccttttctt tttataattc tccgaaaatc cttttaacct gtgctacatt tgcgcatatg
     4381 gcattggaat tgttgaataa gtaataaatt atgaccgtct cggcaaaatc catcacatta
     4441 taatttttat cgttgaaaac ccttttttga agctcaaaca tctgattaaa atatggcata
     4501 aactctttaa gaccattgta caccctgtct atatccaaac ctgcaaggat aaggctttct
     4561 tttattcttg ttattgagtc cttgaacctt tcaggatcta cattttctat gcttattgcc
     4621 attacaccct tactgacaag cttgggattg agatagtcaa atattacaag cacatttttt
     4681 ttcaggagac agtctattgc cagccacatt atcttttttt caaattctgt ttcaaagctc
     4741 tttactttat cagcatagat ggacaagtcc gccgaaacaa gcaacgtttt ggcagaattc
     4801 attatatcta tcctaaaaag attgtctttc cgaatcatgt aatccacaag actttcgctg
     4861 attatcttta tgttctccag agagtcgttg aaggagaaaa gttctttttc atttattctt
     4921 atagaacgtt caaacttttc gaaaagaatt tttatttcct gtccctgaag aaaaaagtca
     4981 atatggttgc aaaaatcttc ccattccatt tcaagatcga tttttttggg agtatactcg
     5041 aagcctgtga agctgcaaac actttttttg agggcatcca ctctttcatc attactagat
     5101 tcgttatcag attcccagat ccacataacg tcgttgaaat ctatcagtat tgcatatcct
     5161 tttccgtttt ccctggacag acccttaaaa aacacaatag aaggatacga aaaaagtttc
     5221 gtatcctccc catagacaaa agaaactatt gagtcaggaa tattaatatt ttcaaatatg
     5281 tccgccagag gcatatccta gcctcctctc ttttattgat aaagccttga tacatcacct
     5341 atttcactga acataccgga aagggtcttc aaaaacccta gcctgttgag tcttatactc
     5401 tcatccctgt ccattacgaa tacaccttcg aaatagttgt ctatctgtgg tttcatctct
     5461 atgagaatat ctattacacc cttaaaattc atttctttga cgcagctttc ataacctgat
     5521 ctcacttcaa gatacttctg aaaaagctcc ttttcctgct cgagaatgaa aagtcttccc
     5581 tgatactgca gtgagttatg actcttggaa atattatata ttctctgaaa agcagtaatg
     5641 aaatctttaa acttatcaag acttaaatac tcttttatgg actgtgccgc cagatatgcc
     5701 ctcagaggtc tgttccagtt tacaagcact gcatttatga cgtcgcgcgg catctcttct
     5761 tttaaaagaa tagtttcaaa acgactttta aaaaactcat ccatcttttc gcagtcatac
     5821 ttcatattga gattatccga agcgatctta agaatatctt ttatatcaaa gtcccattca
     5881 ttgtctataa gtattctaat aattccaaaa gcttttctac gcaaggcaaa aggatctttg
     5941 gaaccgctcg gaattttgtt tatgccaaag tatccggcaa tatcatcagc cttatcagct
     6001 attgaaacaa tattggcggt gatattggat gaggttctgt ctgactcatc ttttggctga
     6061 taatgctcct gagcggcagt acatatatcc tcctcttcat tttttgcttt taggaatatt
     6121 cttcccatag agcctacaac ttcaggaaat tcaaaaacaa catttgaagt tatatctatc
     6181 ttacataata atccggctct ttttattttt tcaacttcat tacccttgaa tcccattctt
     6241 tcggctataa ttgcgcatat ggcagatatt ctttcagctt tgtccttata cgttccgagc
     6301 ccctgctgat aggttacccc tgataactgc tcaaatctct cctgtacaac cgctttaagg
     6361 tcatcattat agtaaaatgc cgcatcatca agacgtgcat ttataaccct tgaatatcct
     6421 gtaactacat tcttctctct agagtagccg tcctgaaaag atatatactt attggtagct
     6481 ttaccgtctt tcagagcaag aaagcttctc tgatgatgtt taatagttac ggtagttatt
     6541 tcaggtggaa gatatgaata cttctcatca aagcttccta caaccgctgt gggatactct
     6601 gtaagctttg caatttcatc tatgagctct ttatcgcggt cacatgtata accgctttct
     6661 ttttcgattc ttgaaagttc ggtacttatc ttttcaactc tctctgaaag cctggcaata
     6721 acaaaagagc tgcgcagctt tgaaaagtac tcatccgcat tgttaatgat ctcttccctg
     6781 ccaaaaaaac ggtgtccgta tgttttgttc gatgattttt taccaaaaat ttctatattc
     6841 attatctcat cattatacag agccagaacc caatggacag gtcttacaaa agagtactct
     6901 cctttacccc agcgcatggg ctttttaaag ttcatcttag tgattgcagg tccaagtatc
     6961 tgttcaaaaa gctctttggt ttttttgcct tttttatcaa tcttcacatg ggcatattca
     7021 atgccgttta tctctttgaa gataacctga tcgacggttg ctcccgatga tttaagaaaa
     7081 ccctcaaagg ctctggaagg actgccgtcc tctgcaaagc aaagattccg tgcaggtcct
     7141 ttcttttcgg taaacgtgtc cggttcttta tctgaaagtc cgcaaacaac cgctccaaac
     7201 ctccttggag caactactgt ttctatatcc gaaaactcaa tatttcttgt ctttaactct
     7261 gatcttataa tttcttcaag ctggagctct atgtttgaat actcagaaga tggaagttcc
     7321 tcgaccccta tctcaaaaag taatttattc atttgaattt cctccgttta ctgcttttac
     7381 atatgtgctc gcacactttc tggacatcga tcttatatcc agaatatact tctgtctttc
     7441 atttacgctt atcgcaccgg cggcatccaa aaggttaaat gaatgagctg ttttggcaag
     7501 aaaatcatag gccggaagat aaagtccttt ttctataagt ctttcaaact catttttgta
     7561 aagtccgtac agcttattta aaagttcggt atcagattgt tcaaagttga acactgaaaa
     7621 ctgtctttca ttttctttat atagatatcc gtaatcgacg gtatcgttcc atttggtatt
     7681 gtatacattc tctattccct g
//
LOCUS       DAOSHR010000097         7686 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_8208_length_7686_cov_5.749312, whole genome shotgun sequence.
ACCESSION   DAOSHR010000097 DAOSHR010000000
VERSION     DAOSHR010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 7686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7686
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8208_length_7686_cov_5.749312"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /locus_tag="PLU28_06475"
     CDS             <1..610
                     /locus_tag="PLU28_06475"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOG34742.1"
                     /translation="QYDKIISVHLSSKLSGTVNSANLAAKEFEPRRITVVDSLLTSWA
                     LGFLILHLKDRIKDGAQDISELAGLAGEFHKKTRVFFSVGNLEYLHKGGRIGKGKALL
                     GSILQLKPILQLKDGEIHPYKNIRGMNGVLKELVNLAVDEGKQPNGIAIVSTGNASIA
                     SYATERAKEKNIDISKIRTGYIDVVLGLHLGPDSIGVITYYE"
     gene            679..3192
                     /locus_tag="PLU28_06480"
     CDS             679..3192
                     /locus_tag="PLU28_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOG34743.1"
                     /translation="MKKTFLIVSFFLLLVFEGFSQSTFRWQDQIIYMVMIDRFSNGNP
                     SNDRQTETGIEAGNVNSKYNGGDLQGLINRLDYIKELGVTAIWITPPVANQWWDDSVD
                     YGGYHGYWARDFSKIDEHFGTNELYTEFVKKAHEKGLYVIQDIVPNHTGNFIIYSNGK
                     YSLNEKSIPVSKPQQYPFNMNDFNDPKQRELAIYHFPSLIGELDIYNTSFSDLDDINT
                     ENPQVRKVLKEIYGNWIETAGIDGFRVDTAKYVEKEFWQDFFKGENNIYEKAQSLGKD
                     NFIAFGETWISPNPYENTADIEINKYFDYGFNSMLDFGLLTDIKRVFKEGKPTSYLEY
                     RLTQRQEVYKDPSMLVTFVDNHDMDRFLKGATINDLKIALTLLYTIPGIPQIYYGTEQ
                     GFTETRAAMFKEGFSSMGEDHFNSNSDLFKFIQAINKMRKEIPTFRYGRIKVLFSDST
                     GPGILCYSIEYENEKYLILMNTNSKRKYATGIKLEIQDGTVLKPVMSMNVPQNDLVYE
                     GKLDTSLNEKSFIIYKVTDEKATLKPKTVSVQLDMQLQGKTFSDDFYIKGRANGAKSV
                     RLIIDGDDKEYAVINLSMKENEEWSIPVKIADFTPGKHTAFVKAFGKTPLFSDYSPTV
                     YFSLDIPQNELAAVEDPLGDDNGPQGKYSYPKDLTFKNQQDMLGARVQQIGTMLSIEI
                     TMRELSDVWGPANGFDHVTFQIFLDSPNKKGVTALPKQNALMPAGLDWDYQLYITGWS
                     CSAYNSTGAGTDSYGLPVTPAPGIKVEKQFSKITFSLPLSVIETNTMKNWNIYITTYD
                     YDGIEAVLRPISPQGGQWAYSGGQTDSPKIMDDLLINIK"
     gene            complement(3220..5502)
                     /gene="fliD"
                     /locus_tag="PLU28_06485"
     CDS             complement(3220..5502)
                     /gene="fliD"
                     /locus_tag="PLU28_06485"
                     /inference="COORDINATES: protein motif:HMM:NF018852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HOG34744.1"
                     /translation="MADNPYLGSMQMAGLTGIDTKSMINKIMEKKLVPVEREQTKFDK
                     LSYQQKAWQAVDKKLSDFWSYLADFRVQSTLIPKKVTSADEKVITVTAQPAAINRSLY
                     VNVVQMASPSKYTAASGPNFIRDDVLLTKKFSELSYTTTPSFASGDPSVNTFTIKTVN
                     SNGNITKNVSIDMAALSTGDPTVQQILDKINTEFSGTMTAELNNGKLQLVKQGDTNKI
                     ILGSSSDTSNFFNVFKLNGAADSTGKVITSVSGEVVGGTYPTPADDTLNNLPLIPGSI
                     TFSLNGGLQTLKDDGGGNLKKVDGTVVGFINYTTGKFSVFSDSSLDGKSMDSVSYKYQ
                     TSTALSGLESTEPVWSVGQSKAFSNYVSGVSSIENTITVNGVDITFTSSDSLTTLIAK
                     INGSQSGATASVDSSGRFFVENREGGPADISISGKDSGGKKLLVALKMLEPDEATLAG
                     TISAGEAAIVKVSSSASGTGAMTVTSWTNNVVFEDITINLKSLSETSSTYTKIDVEQD
                     VDKSVEKIKEYVTKYNELMDFLYVRYKKTPVSTTIDVSSPTRPKDPDAMTEDMTEEQK
                     LEVVLRGDNNLQNIFMKMRSISYGTVDWYSEDKPKYSSFSQIGIINGDAGKTYENTLK
                     GHLSVDESTLKAKLKENPIDVWNIISEEKEVVVGYDSTNKPIYAKNSGVVTQVKDYIW
                     QVTKYSGTIDQISGTNGTIGKEMNRIAKWLVNAINKLQKKEADLYQTFSKMEQAVSKM
                     SIQGSYLLSKLGNNSSNSNQ"
     gene            5656..6189
                     /gene="fliS"
                     /locus_tag="PLU28_06490"
     CDS             5656..6189
                     /gene="fliS"
                     /locus_tag="PLU28_06490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HOG34745.1"
                     /translation="MPENTNNAKVITNGTYSKFIKPQIINNNQVRVDQLSQKPREEPK
                     ERRDPAEQYLENSILTASPQELVLMLFNGAIKFMNQYIDSASKKEYANAHTAIIRAQD
                     IFIELMSTLKPEYEISGNLFGLYTFILDNLEKANFKKETEPVSQMIELTKDLRDTWEE
                     IVKNPNGKPPENQTEKQ"
     gene            6221..6412
                     /locus_tag="PLU28_06495"
     CDS             6221..6412
                     /locus_tag="PLU28_06495"
                     /inference="COORDINATES: protein motif:HMM:NF025436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfB family protein"
                     /protein_id="HOG34746.1"
                     /translation="MDIYGINANNSVLQQAVSLRVMKMAVDTKKEGADMLVQSMQETT
                     QAMHQALNPFLGNNLDIYA"
     assembly_gap    6450..6549
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6550..7641
                     /locus_tag="PLU28_06500"
     CDS             <6550..7641
                     /locus_tag="PLU28_06500"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOG34747.1"
                     /translation="QEGRSPIFLRGWDESSAQVTRGFIDEITVDTPVILIRRCGHKAV
                     VNKPVIDTVDFSTESSYVDPASGTVTENAIKVLYSAIKENFDIKKAFEHAVGYLTSKG
                     YGFVHSDDLHGITPEELPFENSKLKIFEKVAVNSCDELFSFSEQGYFEYFRSVKIYVD
                     GSFGARTALLSQKYNDIESLGQKVWNKDQIAQALNFCEEKNLHLAMHAIGDGAIDEIL
                     GAMEKVNPHKLHRIIHASLVRDDQLERIASHPVVLDVQPAFIESDKTLIPLCLGERAA
                     LCYRFKDFFTHSIPVTFSSDAPVETPDWVRDLSILSENGVSLRQSIVSMTYGASSIDG
                     FERDPLTTGRYLVFKCDPFEKISIPQILG"
BASE COUNT         2343 a         1580 c         1499 g         2164 t
ORIGIN      
        1 acaatacgat aagattatct ctgttcatct ttcttcaaaa ctcagcggaa cggtaaactc
       61 tgcgaatctt gcagcaaaag aatttgagcc cagacgaata accgtcgtag atagtctgct
      121 caccagctgg gcactgggct ttctgatact tcatttaaaa gacagaataa aagacggagc
      181 acaggatata tcagagctcg caggtttagc cggagagttt cataaaaaaa cacgagtgtt
      241 tttttccgtt ggaaatcttg aatatctgca caagggtgga agaataggaa agggaaaagc
      301 tcttttgggc tctatcctcc agctcaaacc tattcttcag cttaaagacg gagagataca
      361 tccttataaa aacatccgtg gaatgaacgg tgtccttaaa gaactggtta atctggcagt
      421 agatgaaggg aagcagccaa acggaatcgc tatcgtttcg acaggaaacg cttcaatagc
      481 ctcctatgca actgaaagag caaaagaaaa aaatattgat atttcaaaaa tacgtacagg
      541 ctatatcgat gtcgttcttg gacttcatct cggacctgat tcgataggag taatcaccta
      601 ttacgagtag attatttttt tataaaaaat tattatataa taatattact ttcaatcttt
      661 ttcaggggag gagtataaat gaagaaaact tttctcatcg tttcattttt tttattgctt
      721 gtttttgaag gattctctca atcaactttc agatggcagg atcagatcat atacatggtt
      781 atgatcgacc gtttttcaaa cggcaatcct tccaatgaca gacagaccga aacaggtata
      841 gaagcaggaa atgtaaactc aaagtacaat ggcggagatt tgcagggatt gatcaaccgg
      901 ctcgactata taaaagagct tggcgttacc gctatctgga ttaccccgcc tgttgccaat
      961 cagtggtggg atgactctgt agactacgga ggataccatg gctactgggc acgcgatttt
     1021 tcaaagatag atgagcactt cggaactaac gaactttata ccgaatttgt aaaaaaagcc
     1081 catgaaaagg gtctatacgt tattcaggat atagtaccca atcacaccgg aaactttatt
     1141 atatacagca acggcaagta cagtcttaat gaaaaatcca tacctgtcag taagccgcag
     1201 cagtatcctt tcaatatgaa tgatttcaac gatcccaagc agagagagct cgccatatac
     1261 cactttccat ctcttatcgg tgaattagat atctataata catccttttc agacttagac
     1321 gacatcaaca ccgaaaatcc gcaagtacgg aaagttctta aggaaatcta cggaaactgg
     1381 atagagacag ccggtataga cggatttagg gttgatacgg ccaaatatgt ggaaaaagag
     1441 ttctggcagg attttttcaa gggtgaaaac aatatttacg aaaaagccca atcactggga
     1501 aaagataact tcatagcttt cggagaaaca tggataagcc ccaatccgta tgaaaacact
     1561 gccgacattg aaataaacaa gtactttgat tatgggttca actcaatgct tgactttggt
     1621 cttctgaccg atataaaacg tgtcttcaaa gaagggaagc caacctcata ccttgagtac
     1681 agactcactc aaagacaaga agtgtacaaa gatccgtcca tgctggtaac ttttgtggac
     1741 aaccatgaca tggacagatt cctcaaggga gcaaccataa atgaccttaa gattgccctt
     1801 acgcttttat ataccatacc aggaatacct caaatatact acggtacgga acagggcttt
     1861 acagaaacac gagcggccat gttcaaagaa ggattttcaa gtatgggaga ggatcatttt
     1921 aatagtaatt ccgatctttt taagtttatc caggccataa acaaaatgag aaaagagatc
     1981 ccgaccttca gatacggtag aataaaagta cttttttctg acagcacagg accaggcata
     2041 ctttgctaca gcatcgagta tgaaaatgaa aaatatctta ttcttatgaa tactaattca
     2101 aaaaggaagt acgctaccgg aataaaactt gaaattcagg acggtacggt tttgaaacct
     2161 gttatgtcta tgaatgtacc tcaaaatgac ctggtttacg agggaaaact tgatacctcc
     2221 cttaacgaaa aatcctttat aatctacaaa gtaactgatg aaaaagctac tctcaagcct
     2281 aaaaccgttt cggtacagct cgatatgcag ctccagggaa aaaccttttc agatgacttt
     2341 tatataaaag gaagagctaa cggagcaaag tcggtaagac ttatcataga cggagacgat
     2401 aaagagtatg cggttataaa tctttccatg aaagaaaatg aagagtggag cattcctgtt
     2461 aagatagccg actttactcc aggcaagcat actgcttttg taaaagcctt cggaaagaca
     2521 ccactctttt ccgattactc tcctacggtt tatttttcac ttgacattcc acaaaatgaa
     2581 ctcgcagccg ttgaagatcc attgggcgat gataacggcc cgcaaggaaa atactcttat
     2641 ccaaaggatc ttacctttaa gaatcagcag gatatgctag gggcaagagt tcagcagatc
     2701 gggacaatgc tgagcattga aataactatg cgtgaactca gcgacgtttg gggtccggca
     2761 aacggttttg accacgtcac ttttcaaata tttctggaca gtccgaataa aaaaggagtc
     2821 acggctcttc caaagcagaa cgctctgatg cctgccggtc tggactggga ttaccagctc
     2881 tatataaccg gatggtcttg ttcggcatac aacagtaccg gtgcaggaac tgacagctac
     2941 ggtctgcctg ttactccggc tccgggaata aaagtagaaa aacagttttc aaaaataacc
     3001 ttttcacttc ctctttcggt aattgaaacc aacaccatga aaaactggaa catatacata
     3061 accacttatg attatgacgg aatagaagcc gttttaagac caataagtcc tcagggagga
     3121 cagtgggcat actccggcgg tcagaccgat tctcctaaga taatggatga tcttttgata
     3181 aatataaaat aaacaagagg cgtatgcctc ttgtttattt tattgattgc tgttactact
     3241 gttatttccc agcttgctca aaaggtacga accttggata ctcattttgg aaactgcctg
     3301 ctccatctta gaaaaagtct ggtaaagatc cgcttctttt ttctgaagtt tattaattgc
     3361 atttacaagc catttggcaa tcctattcat ttcttttcct atagtaccgt tagtacccga
     3421 gatctgatct atcgtaccgc tgtactttgt aacctgccat atataatcct tgacctgggt
     3481 aactactcct gagttcttgg cataaatggg tttattggtt gaatcatagc ccacaaccac
     3541 ttctttttcc tcggatataa tattccatac gtcaatggga ttttccttaa gcttggcttt
     3601 aagagtggat tcatcaacgg acagatgacc tttgagcgta ttttcataag tttttccggc
     3661 atctccgttt attataccta tttgtgaaaa tgaagaatac ttgggtttat cttctgaata
     3721 ccaatccacg gtaccgtatg aaatgcttct catcttcatg aaaatattct gaagattgtt
     3781 gtctcctctg agaaccactt caagtttttg ctcttcggtc atatcctcgg tcattgcatc
     3841 aggatctttt ggtctcgtcg gactgctgac atcgatagtc gttgataccg gtgtcttttt
     3901 atatctcaca taaagaaagt ccataagctc attgtacttt gtaacgtatt cttttatctt
     3961 ttcaactgat ttatctacat cctgttctac atcgatctta gtatatgttg aagatgtctc
     4021 cgaaagactt tttaggttta tggttatgtc ttcaaaaaca acattgttgg tccaggaagt
     4081 cacagtcata gcacctgttc ccgatgctga tgaagaaact ttaacaattg cagcctcacc
     4141 ggcgcttata gtacctgcaa gagttgcctc gtcaggctca agcatcttaa gagctacaag
     4201 aagcttcttt ccaccgctgt cctttccgct tatacttata tcagcaggac cgccttccct
     4261 gttttcaaca aaaaaccttc cgctgctgtc aacagaagcc gtagcaccgg actgagatcc
     4321 gtttatctta gcaataagag ttgtaaggga atcacttgat gtaaaagtaa tatcaacacc
     4381 gtttacagta atagtatttt ctatcgagct taccccggaa acatagttgc tgaaagcctt
     4441 agactgtccc acagaccata cgggctctgt gctttccaga ccgctgaggg ctgtggaggt
     4501 ctgatattta tagctcacgc tgtccatgct tttcccatct aaggatgaat cgctgaatac
     4561 actgaactta cctgtagtat agtttataaa accgactacg gtaccgtcaa ctttttttaa
     4621 gttgcctccg ccgtcatctt ttaaggtctg gagtcctccg ttgagactga aagtaatact
     4681 tccgggtatt agaggaagat tgttcagagt atcgtctgcg ggagtaggat acgttccccc
     4741 tacaacttca ccgcttaccg aagttatgac ttttcctgtt gaatcagctg caccgttaag
     4801 cttaaaaaca ttaaaaaagt ttgatgtatc cgaacttgat cccagtataa ttttatttgt
     4861 gtctccctgc tttacaagct gaagctttcc attgtttaac tccgcagtca tcgttccgga
     4921 gaactctgtg tttattttat ccagtatctg ctggacagta ggatctcctg tcgaaagcgc
     4981 cgccatatca atactgacgt ttttagttat attcccgttg gaatttactg tttttatcgt
     5041 aaaggtattt accgacggat ctccgcttgc gaaagaagga gtggtggtat acgatagctc
     5101 actaaatttt ttggtaagca gaacatcatc ccttataaag ttaggaccgc tggctgcagt
     5161 atacttggaa ggtgatgcca tctgcacaac attaacgtat aaggatctat ttatagctgc
     5221 cggctgtgcg gtaactgtta tgactttttc atcagcggac gttaccttct tgggtataag
     5281 agtactctgc actctgaagt cggcaagata cgaccaaaaa tcactaagct ttttatccac
     5341 agcctgccag gccttctgtt gatatgagag cttatcaaac ttagtctgtt ccctttccac
     5401 aggaactaat ttcttttcca ttattttgtt tatcatagac ttggtatcta taccggtaag
     5461 accggccatc tgcattgaac caagatatgg attgtcagcc attgaatttg tacctcctga
     5521 tccatgattt atttgaacat ctaagtttat tatcgactgt aactgttggt tactttatgc
     5581 tataatttta atatactgct tttgcattta cggacgataa aatgatatga ctatttagaa
     5641 aaacggggtg aaattatgcc ggaaaatacc aacaatgcaa aagtaatcac taacggaact
     5701 tacagtaagt ttataaaacc gcagataata aacaacaacc aggtacgcgt cgatcagctc
     5761 tcacagaaac cacgggaaga accaaaggaa agacgcgatc cggcggagca gtatcttgaa
     5821 aattcaatac ttactgcaag tcctcaggaa ctggtgctta tgcttttcaa cggcgcaata
     5881 aagttcatga accagtatat tgactctgct tcaaaaaaag agtatgcaaa tgcacacaca
     5941 gcgataatac gtgcccagga tatattcata gaactaatgt ctactcttaa gcccgaatat
     6001 gagatttcag gaaatctttt cggtctttac acttttatac ttgacaacct tgaaaaagcc
     6061 aactttaaga aagaaaccga gccagtttca caaatgatag aacttaccaa agatctgcgc
     6121 gatacatggg aagaaattgt taaaaatccg aacggaaaac cacctgagaa ccagacagaa
     6181 aaacaataat attttctaat tggtgaaagg agggagcagc atggacattt acggaatcaa
     6241 cgcaaacaac tccgtcttac agcaggctgt atcgcttaga gttatgaaaa tggctgttga
     6301 tacgaagaaa gaaggggctg acatgcttgt ccaaagcatg caggaaacaa cacaggcaat
     6361 gcatcaggct ctgaatcctt tccttgggaa caaccttgat atttacgctt aacacctgct
     6421 atggttgccc gtaagggcaa cttttttatn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6541 nnnnnnnnnc aagaaggaag atccccaata ttcctacgag gctgggatga atcaagcgcc
     6601 caggttacaa ggggatttat cgatgaaatc actgttgata caccggttat acttatcaga
     6661 agatgcggcc ataaggcggt ggtaaataaa cctgttatag acacggtgga cttctctaca
     6721 gaaagctcct atgttgaccc tgccagcggt accgtcactg aaaatgcaat aaaagttctt
     6781 tactccgcca taaaggagaa ctttgatatc aaaaaagctt ttgaacatgc agttggctat
     6841 cttacttcaa aaggctacgg atttgtacat tccgatgatc ttcacggaat aacccccgaa
     6901 gaactcccct ttgaaaattc caaactcaag atattcgaaa aagtagcagt aaatagctgt
     6961 gatgagcttt tctcattcag cgaacaggga tactttgaat acttccgctc agtaaaaata
     7021 tacgtggacg gttccttcgg tgcaagaact gccttactct cccaaaaata taacgatatc
     7081 gaaagtctgg gacaaaaagt ttggaataaa gaccagatag ctcaagcact taacttctgc
     7141 gaagaaaaga accttcacct cgcaatgcat gctataggag acggagccat tgatgaaata
     7201 ctcggtgcaa tggaaaaggt aaacccgcat aagctccaca gaataatcca tgcttctctt
     7261 gtacgtgacg accagcttga acggatagct tcccatccgg tagtacttga tgttcagccg
     7321 gcttttatag agtctgacaa aacactgatc cctctctgtc tgggagaaag agctgctctt
     7381 tgctataggt tcaaagattt cttcactcac tctatccctg tcaccttttc ctcagatgcc
     7441 ccggtggaaa cacctgattg ggtaagagat ttgagtattc tttcagagaa cggtgtttct
     7501 ctgaggcaaa gcattgtttc tatgacatac ggagcatcgt caattgatgg ctttgaaagg
     7561 gatccgctca caacaggaag atatcttgta tttaagtgtg atccatttga aaagataagt
     7621 atcccacaga ttttaggatg aattagcatt ttttgttttc ttttgcttaa aaaataaatt
     7681 acgaat
//
LOCUS       DAOSHR010000098         7672 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_8239_length_7672_cov_5.559932, whole genome shotgun sequence.
ACCESSION   DAOSHR010000098 DAOSHR010000000
VERSION     DAOSHR010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 7672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7672
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8239_length_7672_cov_5.559932"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(33..992)
                     /locus_tag="PLU28_06505"
     CDS             complement(33..992)
                     /locus_tag="PLU28_06505"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOG34748.1"
                     /translation="MAQKYYIGLDIGGTKILGGLFDENCKMVKRSKKSTKSSQGADVI
                     VGQICKVIDDLLEDSLDGKLVAIGAGCPGIINSKEGVVVFSPNMNWGENFPLKEKMEK
                     KYKVPFFLGNDANILAMGEYKFGAGAGCKNIIGIFIGTGIGGGIIINGKLYEGAVGAA
                     GEIGHTIVLPDGPLCGCGSRGCLESLASRTAMQRDILSQLKKGRTSVITEYMDKEDKM
                     IKSSHLKNTYDKGDELVIEVVDKACYYLGISVGSLMNVFNPDLVIFGGGVIEALGESM
                     MKKIKVTAERYCMPKIFESCKFEIGKLGDDAGLYGALSLAMEQ"
     gene            complement(1110..1466)
                     /locus_tag="PLU28_06510"
     CDS             complement(1110..1466)
                     /locus_tag="PLU28_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOG34749.1"
                     /translation="MTVIISTIILLGVMGFGFGIFLAFAAKKFEVKEDVRTLAIQSSL
                     PGINCGACGFPGCSGFAKAYIENRAKNDGCLPGRKSGVPQKLEKISKLTDDQLQKFFE
                     ETEGDIEKTIKKIMEN"
     gene            complement(1480..2055)
                     /gene="rsxA"
                     /locus_tag="PLU28_06515"
     CDS             complement(1480..2055)
                     /gene="rsxA"
                     /locus_tag="PLU28_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HOG34750.1"
                     /translation="MRLLLIFISAVLINNFVLSRFLGICPFLGVSKKMDSAVGMSIAV
                     IFVITLSSALTWVLNQLLISLGLGFLTIIVFILVIAVLVQFVEFFIKKTSPSLYQGLG
                     IYLPLITTNCAVLGVALINSQQNYSFIESVVNGFSSAVGFSMALIIFAAIRERMEFSD
                     VPAPFKGTAISLITASLLSLAFMGFSGLVKL"
     gene            complement(2052..2681)
                     /locus_tag="PLU28_06520"
     CDS             complement(2052..2681)
                     /locus_tag="PLU28_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HOG34751.1"
                     /translation="MADIKNLTKGLIKENPLFIQVLGTCPALATTTNAKNALGMGVAV
                     IAVLVLSNIVISVIRKMVPNKIRIPIFIVVIASFVTMIDLLMHAFTYDLWKVLGIFIP
                     LIVVNCVLMGRAEAFASKNGVVDSILDGIGMGGGYALALFVMGSIREILGNGSWLGLS
                     FLPQAFKMYIFILPPGAFITFGILIAVINAINLRKEKIEKEKKKAGETK"
     gene            complement(2786..3376)
                     /locus_tag="PLU28_06525"
     CDS             complement(2786..3376)
                     /locus_tag="PLU28_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transporter RnfG"
                     /protein_id="HOG34752.1"
                     /translation="MKDYIKTGLILMAYMVVTGFLVALVYVVTKPKVDEADFKSKVSA
                     IESVLKKAGTDELLVESVPNSAEKLNGYIYKKTENELFKAADGSKVLSPLYKFEEKTH
                     EVYVITFSGVGFGGDVVCVGAFIKQDGVVTLNKIEVINYSNETPGLGANISLEQVKER
                     FSLIPGSGLENGVKVNKDVGVIAQTEEEIKTINHRE"
     gene            complement(3373..4353)
                     /locus_tag="PLU28_06530"
     CDS             complement(3373..4353)
                     /locus_tag="PLU28_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HOG34753.1"
                     /translation="MKLSTSSAPHVRSKDTTRAIMLDVLIALMPAVIVSTLIFGVRAL
                     LMMLFSMVSAEIIEYICVRYVRGRKEFKPDFSAAVTGLLLGMNISVAVEWWQLLIGVI
                     VSIVLAKQVFGGLGSNIFNPALVGRVFMLTSFPIAMTTWYKPYYYLSSADTITAATAL
                     GTLKTAGYEQVVSKFSYLDMFLGNITGSIGEVSFVALLLGFIYLLVRGRIKIMVPLSY
                     TATVLIFSSIFYLVDPVRYGTPLFHILAGGFALGAFFMATDMVTSPMTPKGCIIFGMG
                     LGVITMVIRYFGGYPEGVSYSILVMNALVPLIDRYAKPRIFGTIPKAVKK"
     gene            complement(4355..5677)
                     /gene="rsxC"
                     /locus_tag="PLU28_06535"
     CDS             complement(4355..5677)
                     /gene="rsxC"
                     /locus_tag="PLU28_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HOG34754.1"
                     /translation="MSLLTFKGGVHPPEKKLSENQSIKRLPLPQTVFMYTSNNLGAPS
                     KIIVSVGDKVKTGQLIAQANGLISANLHSSVTGEVVEIKKLVNSATGMNDDVVVINRD
                     GEEEWELLEHSENFLSMSSEQIVDTVKQAGIVGLGGAMFPAHVKMMIPKDKKAEILII
                     NTAECEPYITIDDRIMIEYSEKIITGIRIIMHATGARKAFIGIEDNKPSSYECMKNAA
                     KDYPIEIARLKTKYPQGAEKQLIYGVTKREVPSGKLPIDVGCVVFNVSTVYAVYDAVV
                     NGRPLVERGISLTGEGVKNPGNFIFRIGTKVSELLDYVGLIEEDKIDRIIYGGPMMGI
                     PLSNVELPTFKGNNAITVLTKDVVDSRQVKPCIRCSRCVHVCPMFLQPYLLKHLSDSR
                     QYDTAIENGLMDCMECGCCSYTCPSDIDLVKSFKTAKKVIRAMKQRRP"
     gene            complement(5803..6078)
                     /locus_tag="PLU28_06540"
     CDS             complement(5803..6078)
                     /locus_tag="PLU28_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34755.1"
                     /translation="MKLVISFIMLMLILFFSVMTVETVNKIVMIRRKTETEYQKAVEI
                     INQVERIQSLTLDSFLDEISPKRVYISSDATIYIFEVNGKEFTYINQ"
     gene            complement(6078..7040)
                     /locus_tag="PLU28_06545"
     CDS             complement(6078..7040)
                     /locus_tag="PLU28_06545"
                     /inference="COORDINATES: protein motif:HMM:NF027565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4899 domain-containing protein"
                     /protein_id="HOG34756.1"
                     /translation="MDFYCVKFMASSALTGETYTGYLFGKKKQEPTYNVAVVSRAKIK
                     DYVLPDIKNGYTAFKSDIDIMVYKLYQDDKLLSLNKQFSAFFYTSMKKFNSEIIGTKL
                     YLAVQEGDPYVVKAIIKELLFDWADETEIRIVAEKFDFEDLTWMASAIKKGEEGISGY
                     LNRDDVKSFHEVFPVIDPLDGYPVIKLDVGDPIYTLNIQDKGSTEKLTLESKIISKEL
                     IPNTEYLLLKVDLGNGVMGKTVVNKNLKVMIDKVKLEQSIKERQIIFKDETPNVIMDF
                     ANETMNKKDIYTPRLKSFDLVLVMGFSAIGILLIVLVAIWLGAF"
     gene            complement(7053..>7672)
                     /locus_tag="PLU28_06550"
     CDS             complement(7053..>7672)
                     /locus_tag="PLU28_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HOG34757.1"
                     /translation="FGKNIIPRAVSRHERLFAYNFEGYWRDVGTLTSYLDTNLEILGP
                     LPPLDLHDKSWKIYTQSAELPPAYISENAKVIKSFISEGAEVYGEVENTVVFQGVIIE
                     EGSTVKNSVLMDGCVIGKNTILQDVIVCEKARIGSESRIGVGEFKESLIDKKVYNSNI
                     TVIGYNSVIPQGIIIGKNCVIDNYVTENDFSVKEVQSGEGIIKQI"
BASE COUNT         2324 a         1688 c         1182 g         2478 t
ORIGIN      
        1 acactgttca aatgtttgga cataaaacca tcctactgtt ccatagcgag ggaaagagcc
       61 ccataaagac ctgcgtcatc tccaagttta ccgatttcaa atttgcatga ttcaaaaatc
      121 tttggcatac agtatctttc agcagtaact tttatttttt tcatcatgct ttccccaagt
      181 gcttctataa ccccgcctcc aaaaatcaca agatcaggat taaaaacatt catcagcgaa
      241 ccaactgaaa ttccaagata gtagcatgct ttatcaacaa cctctatcac aagctcatct
      301 cctttatcgt aagtattttt cagatgtgag ctttttatca tcttatcttc tttatccata
      361 tactctgtaa tcacagaagt acgtcccttt ttgagttggc tcagtatatc cctctgcatg
      421 gcggttctag aagccagact ttccaaacat cccctggaac cacagccgca taacggaccg
      481 tccggaagaa ctatagtgtg tcctatttct ccggctgctc ctacagctcc ttcataaagc
      541 tttccgttta taataatacc cccgcctatt cccgttccta taaatatccc tattatattt
      601 ttacagccag ctcccgctcc gaacttatac tctcccatag caagaatatt tgcatcgttt
      661 ccaagaaaaa aaggtacctt atattttttt tccatttttt ccttaagcgg aaagttctct
      721 ccccagttca tattaggcga aaaaacaact actccttctt tagagtttat tatcccagga
      781 catcctgcgc ctatggctac aagtttaccg tctaaactgt cttcaagaag atcgtctatg
      841 accttgcata tctgacctac aatgacatcg gctccttgag aagatttagt gcttttttta
      901 gatctcttga ccatcttgca attttcatca aaaagccctc cgagaatttt agttcctcct
      961 atatccaagc ctatgtagta cttctgcgcc atagcgcacc tccaaaatat agattccttt
     1021 ttcattatat cataattttc tttttcttct gttttcttat tactacttta tatttataaa
     1081 cccattaaaa aaattaaccc cttaaggggt taattttcca ttatcttttt aattgttttt
     1141 tcaatatctc cctcagtttc ttcaaaaaat ttctgaagct gatcatctgt aagtttggag
     1201 attttttcaa gcttctgcgg aacacctgat tttcttcctg gaagacaacc atcattttta
     1261 gccctgtttt caatataagc cttcgcaaac cccgagcatc caggaaaacc acatgcacca
     1321 cagtttatcc cgggaaggct tgactgaatt gccagagttc tcacatcttc ttttacttca
     1381 aacttttttg cggcaaaggc tagaaatata ccaaacccaa agcccataac acccagaagt
     1441 attattgtac ttattataac tgtcatctta tgcctccctt tataacttta ctaatcctga
     1501 aaatcccata aatgcaagcg ataaaagact tgcagttata agcgatatcg ctgttccttt
     1561 gaaaggagct ggaacatctg aaaactccat tctttctctt atagcagcaa aaattatgag
     1621 agccatagag aaaccaacgg ctgaagaaaa accattcaca acagactcta taaaggagta
     1681 gttctgctga gagttgatta gggccactcc aagaaccgca cagttggtag ttataagggg
     1741 cagatatatt ccaagtcctt gataaagcga aggactagtc tttttgataa aaaattccac
     1801 aaactgaact aaaaccgcta ttacaagtat aaatactatt atggtaagaa atcccagtcc
     1861 aagagatata agaagctggt tcagaaccca tgtaagcgca ctggagagcg taatgacaaa
     1921 tataacagct attgacatgc cgactgcaga gtccattttt ttagaaactc caaggaaagg
     1981 acagattcct aaaaatcttg aaagcacaaa gttatttata agtacagcag agataaatat
     2041 cagtaatagt ctcatttagt ctcaccggcc ttcttttttt ctttttctat cttctctttc
     2101 cttaaattaa tggcgtttat aaccgctata agaattccga aagtaataaa ggctcctgga
     2161 ggaagaataa atatatacat tttaaatgcc tgaggaagaa atgacaatcc taaccagctt
     2221 ccgtttccaa gaatctctct tatgcttccc ataacaaaaa gtgcaagagc gtagcctcca
     2281 cccatcccga taccgtcaag aattgaatca acaactccgt tctttgaagc aaaagcttct
     2341 gcacgtccca ttaaaacaca gttcacaact ataagaggaa taaatatacc caataccttc
     2401 cacagatcat aagtaaaagc atgcataaga agatctatca tagtaacaaa tgacgctata
     2461 acaactataa atattggaat tcttatctta ttaggaacca tctttcttat gacagatatt
     2521 actatatttg aaagtacaag aactgctata accgcaacac ccattccaag tgcattcttt
     2581 gcgttggttg ttgttgcaag tgcaggacat gtacccagaa cttgtatgaa aagaggattt
     2641 tcctttataa gtcctttagt tagatttttt atatcagcca tttatatctc gccttccttt
     2701 ctgagatact ctataaccgt atttataccg tttgcaactg ctctgggggt aatagttgcc
     2761 cctgtcatta catcgcttgt ctgaactact ccctgtgatt tattgttttg atttcttctt
     2821 ctgtctgggc aataacgccg acatctttat ttactttaac cccattttca agacctgaac
     2881 cgggaatcaa tgaaaatctc tctttaacct gttcaagcga tatgtttgca ccaagaccag
     2941 gagtttcatt tgaatagttt attacctcaa ttttattcag agtcactact ccatcctgct
     3001 ttataaatgc tccgacacaa acaacatccc cgccaaaacc tactccggag aatgtgatta
     3061 catatacctc atgagtcttt tcttcaaact tatataaagg cgaaagaact tttgaaccat
     3121 cagcagcttt aaaaagttcg ttttctgttt ttttgtaaat ataaccgtta agcttttctg
     3181 cactatttgg aacactttct accagaagct catctgttcc agctttttta agaacggact
     3241 caatggcgga tacttttgat ttgaaatccg cttcgtcaac tttaggctta gtaacaacat
     3301 ataccagagc aactagaaaa cctgtcacaa ccatatatgc cataagtatt aaacctgttt
     3361 tgatgtaatc cttcattttt tcaccgcctt tggaatggtt ccgaatattc tcggtttagc
     3421 atacctgtct ataagaggaa caagggcatt cattaccagt atggagtatg agactccttc
     3481 aggatatcct ccgaaatatc ttataaccat ggttataact ccaagcccca tcccaaaaat
     3541 tatgcatccc ttcggagtca taggagaagt caccatatcg gtagccataa aaaatgctcc
     3601 caatgcaaac ccaccagcga gtatatggaa aagaggagtt ccgtatctaa ccggatctac
     3661 aagatagaat atagacgaaa agataagaac agtagcggta tatgacagag gaaccattat
     3721 ttttattctt ccccgtacga gtagatagat aaatccaaga agcaacgcaa caaaactaac
     3781 ttcaccgatt gaaccggtga tgtttcccaa aaacatgtca agataagaaa acttggaaac
     3841 cacttgctcg taaccagcag ttttaagtgt tcccaaagct gttgcagcag ttatggtatc
     3901 ggcactgcta agatagtagt aaggcttgta ccatgtagtc atggctatag gaaaggaggt
     3961 caacataaaa acccttccga caagtgcagg gttgaaaata tttgagccaa gtcctccaaa
     4021 aacctgtttg gcaagaacta tcgaaactat tacacctata agaagctgcc accattcaac
     4081 agccactgaa atattcattc caaggagcaa gccggtaact gcagcagaaa aatctggttt
     4141 aaactctttt cttcctctta cataccttac acagatatat tcaataatct ctgcacttac
     4201 cattgaaaaa agcatcatga gcaatgctct taccccgaat atcaaagtag aaacaataac
     4261 tgccggcatt agggcaataa gtacatcaag cattatagcc cttgtcgtat ccttgctcct
     4321 tacatgcgga gctgacgatg tgctaagttt catttcacgg cctcctctgc ttcattgctc
     4381 ttatgacttt ttttgctgtt ttaaatgatt ttacaagatc aatatccgaa ggacaggtat
     4441 atgaacaaca accgcactcc atacagtcca taagaccatt ttcgattgcg gtatcgtact
     4501 gtctgctgtc ggaaagatgc ttaagaagat atggctgcag gaacatagga cacacatgaa
     4561 cgcatcttga gcatcttata catggtttta cctgtctgct gtctaccaca tccttggtaa
     4621 gaactgttat ggcgttattt cccttgaatg tcggaagttc cacgttactt aagggtatac
     4681 ccatcatagg accgccgtat atgattctgt caattttatc ttcctctata agcccaacat
     4741 agtcaagaag ctcactcact ttagtaccta ttctgaaaat gaaatttcct ggatttttta
     4801 ctccttctcc ggtaagcgat atgcctcttt ctacaagagg tctgccgttt actactgcat
     4861 catacacagc atacaccgta gaaacgttaa acactacaca gcctacatcg atcggaagct
     4921 ttccgctggg aacttctctt ttcgtaacac cgtatataag ctgtttttca gcaccttgcg
     4981 gatacttggt ttttaatctg gctatctcta taggatagtc ttttgctgca tttttcatac
     5041 attcgtaaga agacggctta ttatcttcta tacctataaa agcttttctt gcacctgtag
     5101 catgcattat aattcttatc ccggttatta ttttttccga gtactctatc attatacggt
     5161 catctattgt aatataaggt tcgcattcag cagtgtttat tatgagaatc tcagcttttt
     5221 tatctttggg tatcatcatt ttcacatgag ccggaaacat tgccccgcca agtccaacga
     5281 ttcctgcttg ctttactgta tctactatct gttcggatga cattgaaaga aaattctcgc
     5341 tatgttcaag aagttcccat tcttcctcac catccctgtt tattacaact acatcatcat
     5401 tcattcctgt ggctgaattg acaagctttt taatttctac aacttcgcct gtaacagaag
     5461 aatgcagatt agcagatata agaccgtttg cctgtgctat cagttgtccg gttttcactt
     5521 tatctcctac agaaacaatt atctttgagg gggcacccag attgttcgaa gtatacatga
     5581 acacagtctg tggcagtggc agcctcttta tggattggtt ttctgataac ttcttttcgg
     5641 gcggatgtac tccgcctttg aaagtaagca aagacattat gcagcctcct ttataattca
     5701 caaaatatat ttctttatta attttactac atactttaat ttgatgcaag tattgcaaaa
     5761 ctttttactg aagatgattt tagaaaaata tacttatttt tatcattgat ttatatatgt
     5821 gaattcctta ccattaactt caaaaatata gatggttgca tccgaggaga tatacactct
     5881 tttaggactt atttcatcta aaaaagaatc caaagtaaga ctttggattc tttcaacctg
     5941 atttattatt tcaactgctt tttgatattc tgtttcagtt ttcctgcgta tcatgactat
     6001 tttattgaca gtttcaacag tcataacaga aaaaaagagt atcagcatca gcataataaa
     6061 acttataaca agcttcatca gaatgctccg agccatatgg ccacaagaac aattaaaagt
     6121 attcctatag ccgaaaaacc cataacaaga accaaatcaa aagactttag tcttggagta
     6181 tatatatctt ttttgttcat tgtctcattg gcaaaatcca ttattacatt aggagtttca
     6241 tctttgaaaa taatctgacg ttcttttatc gattgttcaa gctttacttt atctatcata
     6301 accttgagat tcttattgac aaccgtcttt cccatgactc cattacctag atcaaccttt
     6361 aaaaggaggt actctgtatt tggaataagt tctttggata ttatctttga ttcaagtgtt
     6421 aatttttctg tggatccttt atcctgaata tttaaagtat agatcgggtc tccgacatca
     6481 agttttatta ccggataacc atctagggga tcaataacag gaaacacttc atgaaatgac
     6541 tttacgtcat ctctgttaag ataacccgat attccttctt cacctttttt tatagccgat
     6601 gccatccatg taagatcctc aaaatcaaac ttctcagcta caattcttat ctctgtttcg
     6661 tcagcccagt caaaaagaag ctcttttatg attgctttta ctacataagg atctccttcc
     6721 tgcacagcaa gatagagctt ggttcctatt atttctgaat taaatttttt catcgatgta
     6781 taaaaaaaag cggaaaactg cttgttaagg gataaaagct tatcatcctg ataaagttta
     6841 taaaccataa tatcaatatc tgatttaaaa gcggtatatc cgttttttat atcaggaaga
     6901 acataatctt taatttttgc tctgctaacc acagcaacat tatacgtcgg ttcctgcttc
     6961 tttttcccaa aaagataccc ggtataggtc tcacctgtaa gggctgaaga agccatgaac
     7021 ttaacacaat aaaaatccat ctgggaaccc ccttaaatct gttttattat tccttctccg
     7081 gactgaacct ctttaactga aaaatcattt tcagtgacat aattatcaat gacacagttt
     7141 ttacctataa taattccctg aggtataaca gagttatatc ctataacggt tatatttgaa
     7201 ttatacacct ttttatcaat aagcgactct ttgaactctc ccactcctat gcggctttca
     7261 ctacctattc ttgctttttc acaaacaata acatcctgta ggatggtgtt tttgccgata
     7321 acacacccat ccataagtac ggaatttttt actgtgcttc cctcttcaat tattactccc
     7381 tggaaaacga ctgtattttc aacctcaccg tatacctctg ccccttcgct tatgaaactt
     7441 tttattactt tggcattttc tgatatgtaa gctggcggaa gctcagcaga ttgggtataa
     7501 attttccatg atttatcatg cagatccaaa ggcggcagag gtccaagtat ctccagattg
     7561 gtatcgagat aagaggttaa tgttcccaca tcacgccaat atccttcaaa attatatgca
     7621 aaaagccgtt catgtctgct tactgcacgt ggaataatat tttttccgaa at
//
LOCUS       DAOSHR010000099         7585 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_8374_length_7585_cov_4.076361, whole genome shotgun sequence.
ACCESSION   DAOSHR010000099 DAOSHR010000000
VERSION     DAOSHR010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 7585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7585
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8374_length_7585_cov_4.076361"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1064)
                     /locus_tag="PLU28_06555"
     CDS             complement(<1..1064)
                     /locus_tag="PLU28_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34758.1"
                     /translation="MKKIFLELIVLFFALVCSAVTDVLTEPLNLYGADHTSTGNYMVH
                     NSEVSAVLDEKSGFITAVFFNRNKINIVDAIGPSLKDENLILSQIQPIQDGFLVSFLS
                     ESFSVQTILHLSGKTLFLSTEVKNISQNEKSFSYYDIFDFKNSSPDFYATKLYDRYLL
                     SVSQTGISLGFSTEGENISKRVLVNNRTGVVYAPVKKLIPGESILLERVLRVEETLSD
                     LQQYFGERYGMPLRKYTGKLKEGYGGVKIVAFTPLKQIVSITRTLDDGSFQFMLDEGK
                     YLFQAELGEIRSELRAPQENLIELEIGKIPDPDFVYHPFLTSHSQGEVTVNFKLNMPV
                     PAYVLLYENGVLKEKSEEAP"
     gene            complement(1081..1593)
                     /locus_tag="PLU28_06560"
     CDS             complement(1081..1593)
                     /locus_tag="PLU28_06560"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOG34759.1"
                     /translation="MKISIAGSSDIPKIARVHVDVWKSTYKGIVSDNYLQQLSYEKSA
                     KRLMGIYEKNESIILMAEDGDNVIGFLNGGKERKNDPVYKGEVYAVYILEQYQNKGVG
                     RELIKFFTKYLLENGINNMIIWAFTENKYRCFYEKLGGKALRNDSYTIDGKSLGETGY
                     CWDDISNIRF"
     gene            2077..5382
                     /locus_tag="PLU28_06565"
     CDS             2077..5382
                     /locus_tag="PLU28_06565"
                     /inference="COORDINATES: protein motif:HMM:NF019217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GLUG motif-containing protein"
                     /protein_id="HOG34760.1"
                     /translation="MELIFIDSVKGIAKVQVALGTTQAEVMNELPTKVTITDNKGKNY
                     EITASWALNGYDKDTVGTYTAKGTFTLPVGVSQKTPPVPLEVTVQIEVIRPQLHSVTA
                     LNEKYYVLLGTAKATAMTKLPTSTTVKDNLGKTYEVELNNWVCPTYNANAEADYPASA
                     TFEVPAQINDNSEVSKTVTTKLSVSDNRTLKSIKPVENITVSANTYNTYQIKEVLGLT
                     TQITDSVDATHTVNLTWDVSAYDWTKIGEYTVSATFSLPSNVKNTVPPMELKVSAKIT
                     VISQIGSGTSGDPYIIENLTQLLNVKNGLDKHYKLKENIEIYAAVGTDYWTPLGDETV
                     MFTGSFDGNGSVIVAERIGYDFSVLDVRNNVGIFGVIGATGVVKNLKVKYEMSNGNEN
                     IGVIAGQNKGKIENCTAEKYRFNFNHVGSKNVGGLAGKSEGAIINCKSIQPKFSNWSG
                     ENVGGLVGYNTGSITDSTVSGEAHIVGSASVGGFIGLNSGTVTDCISEMKSNNGSDFS
                     ALGGFVGKNESTGSISYCYVKGSMTGHASSYPSGFAAQNTGTVEYCYSNLTVSDGKMY
                     GFAAPSTAGITKSYWIINSSDQLGGTGGSITSANAKEQASYNTWYFGERWFIDESYPF
                     LLRELKVKYDEGDGTPSNPYVIKNAEQLGGIRYRLDKNYILGNNLILSGNWRPIGISG
                     YDSIRVFTGTLDGNNKTIDGLSTNYSNGGLFAKLSKAAVKNLKFTNVNIKAGATVGTV
                     AGEAGAQYDNTNIVNIENIEISGKVEGTTMVGGVVGVMFRGTLKDITVNADVKGTGST
                     VGGICASTTHVSLNNLIYEGNVSGSEVCGGIVGKIEGKATLIDNLESSGTVQGQRSVG
                     GIAGCVHSAEFDSISSESGISTTGLTSLVEKKGIGGLFGEAKFGVKISNSYYNGSING
                     YGDTGGLIGYADNVTLTGCHSDGTISGLNDDGFKGGLIGRGYASIDRCYSTMGISNSK
                     YSGGLVGVFRENLETKAIKNSYATGTAKAGLVYSAAGRLRFENCYSTVSCLSDAGLIK
                     TIPTSSSGSALNTYWDTEVSGASTSAQYNSAVVGTPKTTAEMKNQVTYVGWDFTSIWT
                     IDPAKNNGYPILR"
     gene            5525..6370
                     /locus_tag="PLU28_06570"
     CDS             5525..6370
                     /locus_tag="PLU28_06570"
                     /inference="COORDINATES: protein motif:HMM:NF004862.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HOG34761.1"
                     /translation="MYRIIYNKEIAKDIYEMHILAPMVARSAKPGQFIILVIDENGER
                     IPLTVVDTDPLAGWVSIVVQAVGHSTHKLTALKESSQIRDFAGPLGNESAFVRKSLQE
                     LKDKKIVFVGGGVGAAPVYPQVKWLSEKNIRTDVILGFKSAEYVILEKEFSALNADIH
                     ICTDDGSIGFRGLVTHKLEPILEHGHIDEVITIGPMIMMKNVSLLTKKYGVPTVASLN
                     TLMVDGTGMCGGCRVTVGGKTLFTCVDGPEFDAHQVDFDQAMSRQGIYRDEEKHRCRL
                     DGEMR"
     gene            6367..>7585
                     /gene="gltA"
                     /locus_tag="PLU28_06575"
     CDS             6367..>7585
                     /gene="gltA"
                     /locus_tag="PLU28_06575"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HOG34762.1"
                     /translation="MIKPRVPVSEQSPLTRKENFSEVCYGYSLEQAFEEACRCLSCKT
                     PACVSRCPVGIDIPGFISCLKNKDLAGAYGIVSKYTSLPAVCGRVCPQEEQCESGCIL
                     GKKSEPVSIGKLERYVGDWALDNDIKPRQAKEKNGYRVAVVGSGPAGLTCAGELAAMG
                     YEVTVFEALHEPGGVLAYGIPEFRLPKEKVVKKEIRRIKEMGVRIQTDVIIGRTLTID
                     ELIIQEKFEAVFIGSGAGLPRFMNIKGENSKGVFSANEILTRINLMKAYSKEYDTPVR
                     LGKRVVVVGGGNVAMDAARASLRMGCEVTIVYRRGEDELPARREEVHHAKEEGIVFNL
                     LSNPVEIITGEDGWVKAVRCIRMELGAPDSSGRRRPVEVKGSEFEIPTDAVIMAIGTS
                     PNPLITSVTPDIKV"
BASE COUNT         2384 a         1282 c         1726 g         2193 t
ORIGIN      
        1 aaaggtgcct cttcagactt ttctttaaga acgccatttt cgtacagtag cacatatgcc
       61 ggaacaggca tgttgagctt gaagtttacg gttacctctc cctgactgtg actcgtgagg
      121 aaagggtggt aaacaaagtc gggatcagga atctttccga tttcaagctc aataagattc
      181 tcctgaggtg cacggagttc ggatcttatt tcgcccagtt ctgcctgaaa aagatacttt
      241 ccttcatcta gcataaactg gaagcttcca tcatcaaggg ttctggttat tgaaacaatc
      301 tgctttaaag gtgtaaaagc tacgatcttt actccgccgt atccttcctt aagctttcct
      361 gtatattttc tgagcggcat accgtatctt tctccaaagt attgctgaag gtcagaaagg
      421 gtttcttcaa ctcttaaaac tctttcaagc agtatacttt caccggggat aagctttttg
      481 acaggagcat ataccacacc ggttctgttg ttcacaagaa ctcttttgga gatattttcc
      541 ccttccgttg aaaaacctag tgagatacct gtttgtgata cactaagcaa gtatctgtcg
      601 tacagcttag tagcataaaa gtcgggactg ctgttcttaa aatcaaatat atcgtagtat
      661 gaaaaacttt tttcattttg cgatatgttt tttacctcag tagataaaaa aagtgttttc
      721 cctgaaagat gaagtatggt ctgaactgaa aaactctcgg ataaaaaaga aaccaaaaaa
      781 ccatcctgta taggctgtat ctgtgaaaga ataagatttt cgtcttttaa tgacggacct
      841 atagcatcaa caatatttat cttgttgcgg ttaaaaaaaa cggccgtaat gaatccgctt
      901 ttttcatcaa gaactgctga aacctcgctg ttatgtacca tataattacc tgtactggta
      961 tggtcagctc cataaaggtt cagcggttcg gtaagtacgt ctgttactgc gctgcacact
     1021 agtgcaaaga acaatacaat aagctcaaga aatatttttt tcatcttttc ctccctttag
     1081 tcaaaaccgt atgttgctga tgtcgtccca gcagtatccg gtttctccga gtgatttacc
     1141 gtcgatggta tatgaatcat ttctgagagc tttaccacca agtttttcat aaaaacaacg
     1201 gtacttgttt tctgtaaaag cccatattat catgttatta attccatttt ccagaagata
     1261 cttggtgaag aattttatca attcacggcc tacgccttta ttttgatact gttcaagtat
     1321 atatacagca tagacttcac ccttgtaaac aggatcattt ttcctttctt ttcctccatt
     1381 gagaaaacct ataacgttat ctccatcctc agccataaga attatgctct catttttttc
     1441 atagatcccc ataagccttt tggcagattt ttcatatgaa agctgctgaa ggtagttatc
     1501 cgaaactatc cccttgtagg tgctcttcca aacatccacg tgtacccttg ctatctttgg
     1561 aatatcagaa cttccagcta tacttatctt caagttccac acctcttttt atataataaa
     1621 aactaagtta taatgccaat ccagtaatat tatatcaatc taaaggcttt tgacaaaact
     1681 gaaatgagag tatctgagaa cctatattca ttcgttggat ctatagtctt ttttcgtgta
     1741 agccttatcg aaaggacaga gtgtaaaaag ataattatga ttgctaaaat gttaatgcat
     1801 aaattcattt taatatatta taatgtcact ttattatatt tgtaagttac aaatagattc
     1861 aagaaactta ttaacgtttt gaagtaacaa tattagttaa atatggtaac tgttttttta
     1921 atgttttgtc caaataattt actttgataa aatgttttag tataatattt ttgattctat
     1981 taataatatt aagggggtac aatgtgaaaa aaagtagcta ggttgttctc attgttttta
     2041 ttgttaacgg tttttctggt ttcatgcggg ccaaaggtgg aacttatctt tattgattct
     2101 gtgaaaggca ttgccaaggt acaggtagct ctgggaacta cccaagcgga ggttatgaat
     2161 gaacttccta ctaaggtgac gataacagat aacaaaggaa agaactatga aataactgct
     2221 tcatgggcat tgaacggata tgacaaagat acagtgggaa catacactgc aaaaggaacc
     2281 tttacgcttc ctgttggagt atctcagaaa actcctccag tgcctcttga agtaactgtt
     2341 cagatagaag ttataagacc acagcttcac tctgtgacag ctcttaatga aaagtactat
     2401 gtactgctgg gaacagcaaa agcaacggct atgactaagc taccgacaag tacgacagtt
     2461 aaggataact tgggcaagac atatgaagtt gagcttaata actgggtatg tccaacatac
     2521 aatgctaatg cagaagcaga ctatcctgca tccgcaactt ttgaagttcc tgctcagatc
     2581 aatgacaact cagaagtatc caaaaccgtt accactaaac tgagtgtttc ggataacaga
     2641 acgctcaaga gcataaaacc agtagaaaat ataactgtaa gtgcaaatac gtataacacc
     2701 tatcagataa aagaagtgtt gggtctcaca acccagataa cggattcagt tgatgctacc
     2761 catactgtaa atctcacatg ggacgtttcg gcttatgatt ggacaaaaat cggtgagtac
     2821 actgtgagtg caactttttc tcttccttca aatgtaaaga acactgttcc tccaatggaa
     2881 ctgaaggttt cagccaagat aacggttatc agtcagattg ggtctggaac ttcaggtgat
     2941 ccttatatca tagaaaacct cacgcagctt ttgaatgtta aaaatggatt ggacaagcat
     3001 tataaattaa aagaaaatat tgaaatttat gcagcggtag gtacagatta ctggactcct
     3061 cttggtgatg agaccgttat gtttacaggt tcttttgacg gcaacggatc tgtcatcgtg
     3121 gccgaaagga taggctatga tttttctgtt ctagatgtga ggaataacgt tggtattttt
     3181 ggcgtaattg gtgcaacagg agttgtaaag aatcttaagg tcaagtatga gatgtccaat
     3241 ggaaatgaaa atataggagt tattgccgga cagaacaaag gaaaaataga aaactgtact
     3301 gctgaaaagt atagattcaa tttcaatcat gtgggttcta aaaatgttgg aggactggca
     3361 ggtaaaagtg aaggggcaat aataaactgt aaatctattc aaccaaagtt ctcgaattgg
     3421 tctggcgaaa atgtaggagg attggtgggt tataataccg gttccataac agacagtact
     3481 gtatctggcg aagctcatat agtgggaagt gcttctgttg gaggttttat tggtttaaac
     3541 agtggaactg taactgattg tatatcagaa atgaaaagta ataatggatc tgatttttct
     3601 gctttaggag gctttgtagg taaaaatgaa agtacaggtt ctataagtta ttgctatgta
     3661 aaagggagca tgacgggaca tgcatcatcg tatccttcag gatttgctgc ccaaaatact
     3721 ggcactgtgg agtactgtta ctccaacctt acagtttctg atggaaagat gtatggtttt
     3781 gccgctcctt ctactgcagg tataactaag agctattgga taataaattc ttccgatcaa
     3841 cttggcggaa caggcggcag tataacttct gcaaatgcaa aagaacaagc tagttataat
     3901 acttggtact ttggtgaacg atggtttatt gatgaaagtt acccatttct tcttcgtgag
     3961 cttaaagtta agtacgacga aggggacggg acaccatcta atccatatgt aataaaaaat
     4021 gcggagcagt tgggcggaat aagatataga cttgataaga actatatttt aggtaataat
     4081 ttaattctca gcggtaattg gaggccgata ggtatctccg gttatgacag tataagagta
     4141 tttacaggaa cccttgatgg aaacaataaa accatagatg gtctttcaac taactatagc
     4201 aacggagggc tttttgccaa actttccaaa gcggcggtaa aaaatctgaa gttcacaaat
     4261 gtaaatatta aggctggcgc aacagttgga acagttgcag gtgaggctgg agcacagtat
     4321 gataatacaa acatagtcaa tattgaaaat attgagatat caggtaaagt agaaggcacg
     4381 acgatggttg gaggcgtagt aggtgtaatg ttccgcggta cgcttaaaga cataaccgta
     4441 aatgctgatg taaaaggtac cggatcgaca gttggcggaa tatgtgcgtc aacgacgcat
     4501 gtcagtctga ataatcttat atatgaagga aatgtatccg gcagtgaggt atgtggagga
     4561 atagtcggaa agattgaagg aaaagcaact cttattgaca atcttgagag ttctggaaca
     4621 gtacagggtc aaagaagtgt tggaggaata gccggttgtg ttcattcggc agagtttgac
     4681 agtatatctt ccgagtcagg tatatcaaca acaggactaa cttcgcttgt agaaaagaaa
     4741 ggcataggag gcctttttgg cgaggctaag tttggagtaa aaataagtaa ttcttactat
     4801 aacggcagca taaacggata tggcgataca ggaggtctta tcggatatgc agataatgta
     4861 acgcttaccg gctgtcattc agacggaaca atttcaggat tgaatgatga tgggtttaaa
     4921 ggcggtctca ttggcagagg ttacgcaagc atagacagat gttattcaac aatgggtata
     4981 agcaattcca agtattcggg aggtcttgta ggggttttca gagaaaactt ggaaacaaag
     5041 gccattaaaa attcgtatgc cacaggtaca gctaaggccg gtctggtata ttctgctgcc
     5101 ggtaggctta gatttgaaaa ctgctactca actgtaagtt gtttgagtga tgcaggactt
     5161 ataaaaacca taccaacatc aagcagtggt tctgcattaa atacttactg ggatacagaa
     5221 gtttccggag catcaaccag tgcacagtat aattcagcag tagtaggtac tcccaaaaca
     5281 acggccgaga tgaaaaacca ggtaacctat gtgggctggg actttacaag tatctggacg
     5341 atagatcctg ccaaaaacaa cggatatcct atattaagat aattacttag aagtttaagc
     5401 ctcccgaaag ggaggttttt tcagatagta tatattaata taaaaataaa aagtatttag
     5461 tttcaaaaaa tacgatagta tgatatattt ttatatggat aaaaagtttg aacaggaggt
     5521 agtaatgtat agaattattt ataataagga aatagccaaa gatatttatg aaatgcatat
     5581 actagcccct atggttgcta ggtctgccaa acccgggcag tttataattc ttgtaataga
     5641 tgaaaatggt gaaaggatac cgcttaccgt tgttgatact gatccccttg cgggctgggt
     5701 atctattgtc gtgcaggcag taggtcattc tacccataag ctgacggctt tgaaagagtc
     5761 ttcccagata agggattttg cagggcctct tggcaatgag tcagcttttg ttcggaaatc
     5821 attacaggag ttaaaagaca agaagatagt ttttgtagga ggaggagttg gagcggctcc
     5881 tgtatatcca caggttaaat ggctgtcgga aaagaatata agaaccgatg taattcttgg
     5941 ttttaaaagt gcagagtacg ttattttgga aaaagagttt tcagcactta atgcagatat
     6001 tcacatatgt actgatgacg gtagtatagg attcagaggt cttgtgactc acaagcttga
     6061 acctatccta gagcatgggc atatagatga agtaataacc ataggaccaa tgatcatgat
     6121 gaaaaatgta tcgctgttga caaaaaaata tggtgttccg actgtggcaa gtcttaatac
     6181 cctcatggtt gatggaactg gaatgtgcgg aggatgcagg gtaacggtag gagggaaaac
     6241 cctatttaca tgcgttgacg gtccggagtt tgatgctcat caggtggact ttgaccaggc
     6301 gatgtccagg cagggtatat acagggatga ggaaaaacac aggtgcaggc tggatggtga
     6361 gatgagatga ttaagccaag agttcccgta agtgagcaga gtcctttgac aagaaaggaa
     6421 aacttttctg aggtatgtta tggctattca ttagagcagg cgtttgaaga agcatgcaga
     6481 tgccttagct gtaagactcc ggcgtgtgta agcagatgtc ctgtaggaat tgatatcccc
     6541 ggatttataa gctgtcttaa aaataaagat cttgctggtg catacggtat tgtttcaaag
     6601 tatacttcac tgcctgcggt ttgtggaagg gtgtgtcctc aggaggaaca gtgtgaaagc
     6661 ggatgtattc tgggtaaaaa atcagaacct gtttccatag gaaagcttga aaggtatgtt
     6721 ggcgactggg ctcttgataa tgatataaaa cccaggcagg caaaagaaaa aaacgggtac
     6781 agagttgcgg ttgtcggcag cgggcctgcg ggtcttacat gtgcggggga gcttgccgcc
     6841 atggggtatg aagtaaccgt atttgaagct cttcatgaac cggggggagt gctggcttat
     6901 ggcataccgg agttcagact tcctaaagag aaagtagtca agaaagagat caggcgtata
     6961 aaagaaatgg gagtaagaat acagaccgat gtcataatag gcagaacgct tacaatagat
     7021 gagcttatca tacaggaaaa gtttgaagca gtttttatag gttcaggggc agggcttccc
     7081 aggttcatga acataaaggg tgaaaacagc aagggggttt tttctgccaa tgagattctg
     7141 accaggatca atcttatgaa agcgtatagt aaagagtacg atacgccagt acgtcttggt
     7201 aaaagagttg ttgtcgtagg tggcggaaat gttgcaatgg atgcggcacg tgcaagctta
     7261 agaatgggtt gtgaggtaac gattgtttac aggcgcggag aagatgagct tccggcgaga
     7321 cgtgaggagg ttcatcatgc aaaggaagaa ggcatagttt tcaatcttct ttcaaatcct
     7381 gttgagataa ttacagggga agacggttgg gtcaaggctg tgagatgtat aagaatggag
     7441 cttggtgctc ctgacagttc aggcagaagg aggcctgtag aagttaaagg ttctgagttt
     7501 gaaataccta cggatgcggt gataatggca ataggtacct ctccgaatcc tcttataact
     7561 tctgttacac ctgatataaa agtaa
//
LOCUS       DAOSHR010000100         7458 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_8556_length_7458_cov_5.298933, whole genome shotgun sequence.
ACCESSION   DAOSHR010000100 DAOSHR010000000
VERSION     DAOSHR010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 7458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7458
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8556_length_7458_cov_5.298933"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="PLU28_06580"
     CDS             complement(<1..481)
                     /locus_tag="PLU28_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34763.1"
                     /translation="MAQKQSNEQKLQQIARDFSEDIKVITLEEEKEKSDIPEEYSSDD
                     YSQTSLHKGEEFEESFETGIAFSKKYYYQVGKTEERYFVVNFSSQNKFKEQLQKVIDS
                     FPVYERLDGDKIKAVAKTSRKQDMSDNIFVLDKDHINNYEILLPDGKIYPISFFTSGK
                     "
     gene            complement(462..4910)
                     /locus_tag="PLU28_06585"
     CDS             complement(462..4910)
                     /locus_tag="PLU28_06585"
                     /inference="COORDINATES: protein motif:HMM:NF016859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34764.1"
                     /translation="MSIFEDFQNPDIKKLFDVFKYKFYDFLKKSESFKQENEKVVKAL
                     KETEIDYKKISEFRDNEGKKIGNICEVTLKVGNDIRKNGEILILTQDEDGYFYNGFRE
                     KMYSPWVVFDKFLSEEEASADVEEESDNEKVVTPDKEKDAGKDKEKILDLKGSIILPG
                     YYLAILTYLMSIICSIENSTKKKTSAENDFVQLSLINTLSNKLGVYKFKLHKIDKIRS
                     LWVKVPVFFEPENLNYIERKTNPYKVVYPSEKVSESIRLPNDTQTNIFDMLETPESEK
                     IGLNLTLVDSDNLSYELSSLNFKPKILEIDENYSLSYATKQIPFLLHSDGARVLMGAK
                     NLKQAVKVVKAEKPIVFTGNEGGELGVNALIAYGLYHGFNFEDGIVVSSGFAEKMKVV
                     VQEKEYFTIKTKNVKSPEKIGTQWKYGNKEILWKVKIGSVVEYGDLLFIEKEGGKEFL
                     HRYTGKYTAEVKQIPDKPEIPVKKRDSNGENNEYYIKIGMLFNVVKPLEVGDKIMGRH
                     GNKGTVSLILDDDLMPKATIGGENKVLDMLLSPLGVVSRMNLGQLYEVHASLAHPQGK
                     KYGPFFNGYDEKQEILKKLKLIGSDDFGRFAVSGYENRLVCGYQYFVRLDHCVRDKIH
                     FVSSAKESELICQPLKGKSRNGGQKIGEMEFWTLLSYNNMELVHLFKGLNSVKKPVTE
                     AEDVYLEILRRAGVNIKTDGFKTEVCAEAGSNKKYSRVISEIFKNSISNRWALKYIYD
                     EQNADKEEFINVLNAFREKNEQSIKNKLKELDGKVFNELKGILEKYSSVLKVIIREKD
                     QTIGDDDIDAFCITPEKRADSLIKDIIEKLEYTSAIKKQFKSLSFQKSGYARSYVVAR
                     RIHNSGRAVITPQPRTEYRKTKLTVDDVILPIEFGIEMLKTLESEEKDFLPQVLKEDA
                     LKGSKEERKKVSEKLNELFAKRWPDRLVILNRQPSLIRHSMQSFYPKFWENYTIGLPI
                     LVCQGFNADFDGDTMAVYFPAIWNEKIKAEAEKMLPSNNPFSMSNSDLNYSIDQDIVY
                     GKYLVQTKNKKVVKADIKKDINGKNAQEIKIYLNDLMCKTLKDSTEKSMTLGIYEIQS
                     NNDECSSMFKIRESQCRGKKEQYNQLYKKIDDKKENFLQGISAQSYYNVKNGVAARAR
                     RTLMDKKLHVADAGYFTRKLTEFLGSIYVDSNFKEYFEIDLKSIGKIKIKSYGKGKIK
                     SYRKIKIKSYGKGKIKSYGKPENFIMYRYVKVDGKELFVDASNFNEIVKVIDKVKLLS
                     PGIRKEKDNTFRISGKYLGKDISTLKSFSDGEMIGITAGHVLGERGTQLSMETFHSGS
                     RGLNMNSISSYILSQAFEETEYMDFLYKLQNYEQNSDQKKKNKSDEVDMLQNYEQNSD
                     QKKKNKSNKVDILNKINPYSIYFEILYNFARFIKTELKIKSFKKFVLNTELRGFLTCL
                     TFESSDTVMKNVMDELQNKKVMIFNETHPRVNYSFFRRDEDGSEAE"
     gene            complement(4897..>7458)
                     /locus_tag="PLU28_06590"
     CDS             complement(4897..>7458)
                     /locus_tag="PLU28_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34765.1"
                     /translation="IQWYIDPEDGYDETRIRLVEQKTASGISKRWVKTSKKKLVCKDE
                     REEKEEIIEIDEAERQKIMGSRSVCKIRHYLCRDPEIVVDEFLTPYGKKYNNFDYKYV
                     MEVELKKVEKSFEEILLENNLNDYIKEEVTGGISNKDIAVQANDFIKEIKYVENRLEG
                     TVTVFFSPGKSIMKVINSKESDKVKKYLENVSILKGLSKESIGGVCAEVDTIDLLEES
                     GFEVSKVVMFLVQDFLENVDGDILNDLLPGLEILNKEKYPNVTESSVYIYYILRNILK
                     IESIDIEAFEGEPAEPQAQKALQEKLWEVLDKYDRQQKVIMDIAPGLKNISIMQVIYS
                     LFKKKSFYYKFEHSQELLEFPAFGFDWDYAYLDEIIHVIESKKLETKMSTEDYLKLPD
                     MITKVYQLNQSKDFEPFYSIDRIGMMYKSKKEIPFGYGENLIKFVKNDDLESYLTQGI
                     KKKWTNMWIGDLIPETVEHSQRHSKRLMDFTVKLINVMGEEEFLGEVPEGEYYNGMSY
                     KTIFYFLLIAAMNVHDLGHTYAYFYFDGEYGNEKKRLILDKYPNFVRDLHNELTISLI
                     REEKLFDILGKGSSFSSESKKVTDLFGEKTDEIIEALILICRYHREHLGIDEDIAIDK
                     KKDICNLFRLDTSPLKKVIKGKIGDEKLSQVVIHAARWLKFIDGTDVQADRIITPQYN
                     IIRKKRTAYEALFLIEKHLLKYDSAEYKNELISLKELLEKEEFLKVKEIKSRLEKMLL
                     EEVNPIIQDMNFLKFYDPRYEELAHIIFKVSQFEHFEKHNAVGAIYPVAFTCNGQAQL
                     VIEIIPNISEQKDAENVESEIKRIYEDIEEELEKAEIKVRGMKLKLGGKKHNEYI"
BASE COUNT         2163 a         1707 c          889 g         2699 t
ORIGIN      
        1 cttttccgga tgtaaaaaag gatatggggt aaatcttccc gtccggtaaa agaatttcat
       61 agttgtttat atgatcctta tccagaacaa atatattgtc gctcatatcc tgttttcttg
      121 aagttttagc cactgctttt atcttatcgc cgtcaagtct ttcataaacc gggaaagaat
      181 cgataacctt ctgaagctgt tctttaaact tattctgact ggaaaagttc accacaaagt
      241 atctttcttc tgtctttcct acctgataat aatacttttt tgaaaaagct atccctgttt
      301 caaacgactc ctcaaactct tcgcctttat gcagagatgt ctgcgaataa tcatccgatg
      361 agtactcctc cggaatatcg cttttctcct tttcttcttc aagagttatt acttttatat
      421 cttcactgaa atccctggct atctgctgta atttctgttc attactctgc ttctgagcca
      481 tcctcatccc tcctgaaaaa agaataattg acccttgggt gcgtttcgtt aaaaatcata
      541 actttcttgt tttgaagttc gtccataacg ttcttcataa ccgtgtcaga actttcaaaa
      601 gtaagacagg ttaaaaaacc ccttagttct gtatttaaaa caaacttctt gaatgactta
      661 atcttcagct cggttttaat aaacctggca aagttataga gtatctcaaa ataaattgaa
      721 taaggattta ttttattcag aatatctacc ttatttgact tattcttttt cttttgatcg
      781 ctattctgct cataattttg aagcatatct acctcatctg acttattctt tttcttttga
      841 tcgctattct gctcataatt ttgaagcttg tataaaaaat ccatatactc tgtttcttca
      901 aaagcttgac tgagaatata cgagcttatt gagttcatgt ttaacccacg agaacccgaa
      961 tgaaaggtct ccatagaaag ctgagttccc ctttctccaa gaacatgtcc tgctgtaata
     1021 cctatcattt caccatctga aaaagatttt agtgttgaaa tgtcttttcc aagatacttt
     1081 cctgatattc tgaaagtgtt atccttttct tttcttatac ctggagaaag gagctttacc
     1141 ttatctatga ctttaactat ctcattgaag tttgatgcat ctacaaaaag ctctttcccg
     1201 tcaaccttta catacctgta cattatgaaa ttttcgggct ttccataaga ttttattttt
     1261 ccttttccat aagattttat ttttattttt ctataagatt ttatttttcc ttttccataa
     1321 gattttattt ttatttttcc tatagacttc aaatcaattt caaaatactc tttaaaatta
     1381 ctgtccacat atatgcttcc aagaaattcc gtgagctttc gtgtaaaata tccagcatct
     1441 gcaacatgaa gctttttatc catgagagtt cttctggctc ttgcagcaac tccgtttttc
     1501 acattatagt agctttgcgc agaaattccc tgcaaaaagt tttctttctt atcatcaatc
     1561 tttttgtaca gctggttgta ctgttctttt ttgcctctgc actgagattc ccttatctta
     1621 aacatactgc tgcactcatc attattactc tgtatttcat atattcctaa agtcatactc
     1681 ttttctgtag aatctttaag tgtcttacac ataagatcgt ttagatagat ctttatctct
     1741 tgggcatttt ttccgttaat atctttcttt atatctgcct ttaccacttt tttgtttttt
     1801 gtctggacaa gatactttcc gtaaactata tcctgatcta tagagtagtt tagatccgaa
     1861 ttactcattg aaaaaggatt atttgaaggc aacatctttt cggcttctgc ttttattttt
     1921 tcattccata tcgccggaaa gtacacagcc atggtatccc catcaaagtc cgcattgaat
     1981 ccctgacaca ctaatattgg caaccctatc gtataattct cccagaattt tggatagaac
     2041 gactgcatcg aatgtctgat aagagaaggc tgcctgttta aaatcaccaa cctgtcaggc
     2101 catctcttgg caaaaagctc attcaatttc tcggatacct tttttctctc ctctttactt
     2161 cctttcaacg catcttcttt caatacttgc ggtaaaaaat ccttttcttc gctctctaaa
     2221 gtttttaaca tctcaattcc aaactcgata ggaagtatca catcatcaac cgtaagcttc
     2281 gttttcctgt actctgtccg gggctgagga gttattacag ctctgccaga gttatgaatt
     2341 ctccttgcaa caacatacga acgagcataa cctgactttt gaaaagacag acttttaaac
     2401 tgcttcttaa ttgcgcttgt atactccaac ttttctatta tatcttttat caaagagtcg
     2461 gctcttttct cgggagttat acaaaaagca tcaatatcat catcaccaat agtttgatct
     2521 ttctccctta ttatgacctt taaaacgctg ctgtactttt ctaatattcc cttcaattca
     2581 ttaaaaactt tcccatctag ctccttaagc ttgttcttaa ttgattgttc gttcttttcc
     2641 ctaaatgcat tcaaaacatt tatgaactct tctttatcag cattctgttc atcatatata
     2701 tactttaaag cccatctgtt gcttatagag tttttaaaaa tttctgaaat taccctgctg
     2761 tatttcttat tagaaccagc ttctgcgcat acctcagttt taaacccatc cgttttaata
     2821 tttactcctg ctcttctcaa tatctccaag tatacgtctt cagcttcagt aacaggtttt
     2881 tttacagagt taagaccttt aaaaagatga accagttcca tattgttata actcaaaagt
     2941 gtccagaact ccatctctcc gatcttttgc ccgccgttac gtgacttacc tttcagcggt
     3001 tgacatataa gctctgattc ctttgccgaa gaaacaaaat gtatcttatc tctgacacaa
     3061 tgatccaatc tcacaaagta ctgatatcca cacacaagtc tattttcgta tccgcttact
     3121 gcaaaccttc caaaatcgtc tgatccaata agcttcagct ttttcaaaat ctcttgcttc
     3181 tcatcatatc cattaaagaa tggtccatat ttcttacctt gaggatgtgc aagactagcg
     3241 tgtacttcgt acaactgccc aagattcatt cttgaaacaa cccccagtgg gctcaaaagc
     3301 atgtctaata ccttattttc gcctcctatt gttgccttag gcatcaggtc atcgtcaaga
     3361 ataagagaaa cagttccttt atttccgtgt ctgcccatta tcttatctcc aacttcaaga
     3421 ggctttacaa cattaaaaag cattcctatc tttatataat actcattatt ctctccgtta
     3481 ctatcacgtt ttttaaccgg aatttcaggc ttgtcaggaa tctgtttaac ttctgccgta
     3541 tacttaccgg tataccgatg caaaaactct tttccgcctt ccttctctat aaaaagaaga
     3601 tctccgtact caaccacact tccgattttt accttccata aaatctcttt attcccatac
     3661 ttccactgag tgccaatttt ttcgggtgat ttcacatttt tagtttttat tgtaaagtac
     3721 tctttctctt gcacaactac tttcattttt tcagcaaatc cagaactcac tactataccg
     3781 tcttcaaagt taaaaccatg gtaaagaccg taagctataa gagcatttac tcccagctct
     3841 cccccttcgt ttcctgtaaa aactataggt ttttcggctt ttacaacctt aactgcctgc
     3901 tttaaatttt ttgctcccat taaaaccctt gcaccatcag aatgaagcaa aaaaggaatc
     3961 tgctttgtag catacgaaag agaataattt tcatctatct ccaatatttt gggtttaaaa
     4021 ttcaaactgc ttaactcata acttaaatta tcactgtcaa ccaaagtcaa attaagtcct
     4081 atcttttcag attcgggcgt ttccaacata tcaaaaatat tcgtttgagt atcgttaggc
     4141 aacctaatgc tttcagatac cttttcagaa ggataaacta ctttatacgg attagttttt
     4201 ctctcaatat aattaagatt ttcaggctcg aaaaaaaccg gaaccttcac ccacaaactt
     4261 ctgattttat ctatcttatg aagcttaaac ttataaacgc ccaatttatt tgaaagcgta
     4321 tttatgagcg aaagctgtac aaaatcattt tcagctgaag tcttcttttt tgtagagttt
     4381 tctatcgaac agataataga cataagatac gtcaatatag caagataata ccccggaaga
     4441 attatcgaac cctttaaatc aagtatcttc tctttatctt tgccagcatc cttctcttta
     4501 tctggggtaa caaccttttc attatcagac tcttcttcta cgtcggcact tgcttcttct
     4561 tcactcaaaa atttgtcaaa aactacccac ggagagtaca tcttttccct gaatccatta
     4621 taaaaataac catcctcatc ctgcgttaga attagtatct cgccgttttt acgaatatca
     4681 ttaccaacct ttaacgttac ttcacagata ttaccgattt tcttaccttc attatcccta
     4741 aactcactta tttttttata atcaatctca gtttctttta atgcttttac aactttctcg
     4801 ttttcctgct taaaactttc tgacttcttc aaaaaatcat aaaacttata tttaaaaaca
     4861 tcgaaaagct ttttaatatc aggattttga aaatcttcaa atatactcat tatgcttctt
     4921 tcctcccaat tttaatttca tgcctctaac ctttatctca gctttttcca actcttcttc
     4981 tatatcctca tatattctct ttatctcgct ttctacgttc tcagcatcct tttgctcact
     5041 aatattagga attatttcta ttaccaactg cgcttgcccg ttgcatgtaa aagctacagg
     5101 atatatagcc ccgaccgcat tatgcttttc aaaatgctca aactgtgaaa ccttgaaaat
     5161 aatatgagcc agctcctcgt atcgtggatc gtaaaacttc aaaaaattca tatcttgaat
     5221 tatcggattt acctcttcta aaagcatctt ttcaagccta ctctttatct cttttacttt
     5281 cagaaactct tctttttcaa gcagctcttt taaacttata agctcattct tatactcagc
     5341 actatcatac tttaaaagat gcttttctat aaggaacaaa gcttcgtatg ccgttctctt
     5401 ctttcttata atattgtact gaggagtaat aattctatcg gcctgaacgt ctgtaccgtc
     5461 tatgaatttt aaccatctcg ccgcatgaat aaccacttga ctaagctttt catcaccaat
     5521 ttttccttta attacttttt taagcggtga agtatctaac ctaaagagat tacaaatatc
     5581 cttcttctta tcgatagcta tatcttcatc tatacccaaa tgctccctat gatacctgca
     5641 gattaatatc aaagcttcga taatctcatc tgtcttctca ccaaaaagat cggttacctt
     5701 cttagactcc gatgaaaaag aactaccctt accaagaatg tcaaaaagct tttcttccct
     5761 gataagactt attgtaagct cattatgcag atcacgcaca aagttaggat acttatctaa
     5821 aatcaacctc tttttttcat taccatactc gccatcaaaa taaaaatatg cataagtatg
     5881 acccagatca tgaacattca tagcagcgat caaaagaaag taaaatatcg ttttataact
     5941 cattccatta taatactcac cttcaggtac ttcgccaaga aactcctctt cccccataac
     6001 gtttatgagc ttgacagtaa aatccataag ccttttgctg tgcctctgag aatgctcaac
     6061 agtctcagga atcaggtcac ctatccacat gttcgtccat ttctttttta tcccctgagt
     6121 aagataactt tccaaatcat catttttaac aaatttgata agattttcac catacccaaa
     6181 cggaatctct ttctttgact tgtacatcat tcctatccta tcaatagaat aaaaaggctc
     6241 aaaatcctta gactgattca actgatacac cttggttatc atatccggaa gcttcaaata
     6301 atcttcagta ctcattttag tttcaagctt cttactctct ataacatgaa ttatctcatc
     6361 aagataagca taatcccagt caaacccaaa agccgggaac tcaagcaact cttggctatg
     6421 ctcaaactta taataaaaac ttttcttttt aaacaaagaa tagatcacct gcataatact
     6481 tatatttttc aaacccggag ctatatccat aataaccttc tgctgccggt catatttatc
     6541 caagacctcc caaagctttt cttgcaaagc tttctgagct tgaggctctg ctggttctcc
     6601 ctcaaaagcc tctatatcaa tactttctat tttaaggata tttctcaaaa tatagtatat
     6661 gtaaacacta gactcagtta cgtttggata cttttcttta tttagtatct ctagccccgg
     6721 cagcaaatca ttcaatatat ctccatctac gttttccaaa aaatcctgaa ccaaaaacat
     6781 aaccactttt gaaacttcaa atccagactc ctcaagaaga tctatcgtat ctacttccgc
     6841 acatacacca ccgatacttt ctttactaag tcccttaaga atacttacat tttccaaata
     6901 tttttttact ttatccgatt ctttactatt aataactttc attatagatt ttcctggaga
     6961 gaaaaaaaca gtaaccgttc cctccaacct attctcaaca tacttaatct cttttataaa
     7021 atcattagcc tgcacagcta tatctttgtt agaaataccc ccggttacct cttctttaat
     7081 ataatcattg agattatttt ccaacagtat ttcctcaaaa gacttttcaa ccttcttaag
     7141 ctctacttcc ataacatact tataatcaaa gttattatac ttcttaccat aaggagtcaa
     7201 aaactcatcc acaacaattt caggatccct gcacaaataa tgcctgatct tacaaacact
     7261 acgagatccc ataatttttt gcctttctgc ttcatctatc tctattatct cttctttttc
     7321 ctctctctca tccttacaaa caagcttctt cttagaagtt tttacccatc tctttgatat
     7381 cccagaagca gtcttctgct ccactaatct tattctggtt tcatcataac catcttcagg
     7441 atcaatatac cattgaat
//
LOCUS       DAOSHR010000101         7359 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_8738_length_7359_cov_4.803669, whole genome shotgun sequence.
ACCESSION   DAOSHR010000101 DAOSHR010000000
VERSION     DAOSHR010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 7359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7359
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8738_length_7359_cov_4.803669"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            449..610
                     /locus_tag="PLU28_06595"
     CDS             449..610
                     /locus_tag="PLU28_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34766.1"
                     /translation="MMKIFNEIFNGSRSYRTESLQKSNFTFELLQNSMLSKTNEHEEL
                     YLRRHTYEK"
     gene            600..722
                     /locus_tag="PLU28_06600"
     CDS             600..722
                     /locus_tag="PLU28_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34767.1"
                     /translation="MKNKKYFIQENIQSKNGRYSAALENIYELSEIYIELSKII"
     gene            868..2610
                     /locus_tag="PLU28_06605"
     CDS             868..2610
                     /locus_tag="PLU28_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG34768.1"
                     /translation="MKKILKYIGKYKLLVFLPFVFLFVSIVLDLMQPFLSKVIVDRVL
                     LNKETSLLGIILVSLATITLVRVILGYFREYTLDYLGVRVATDIKNDLFDHIQTLSFK
                     YFDNMNTGELMSRLTEDVDNIWMALAFGISLFIENSIYFIFAAAILLYLNWKLALISL
                     CIMPILAWLAMKLEKDIEKAFDKISDHAVHINTTAQEDISGIRLVKAFAREKHEILKF
                     LNLNAVNYNLNVEKAKIWGKYYPLIEFLTNISIVLVISFGGMLVIKEDISIGTLVAFN
                     GYLSMLVWPMRMMGWLTNVISQANTSAKKIFGIMNTKADIASTQDCIKPLEIKGDITF
                     KNVSFKYNNSYVLKNINLNIKAGSTVAIMGTTGSGKTSLVSLIGRYYDVTEGSVEIDG
                     KDIRDLDLDLLRSSMSVVFQDTFLFSDSIANNVAFSVKKSSEQSIKDACNMACIDDFI
                     SSLENGYDTEIGERGIGLSGGQKQRLSIARAMIKKSSILILDDSTSALDMETEFQLLK
                     NLNGLKNKPTTFLIAHRISAVKNADIILYLEDGSIKEYGTHNSLLAMKGRYYQVYSDQ
                     FKDYEELEYTEELA"
     gene            2623..4410
                     /locus_tag="PLU28_06610"
     CDS             2623..4410
                     /locus_tag="PLU28_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG34769.1"
                     /translation="MPRNTTSKDEDTLKTSKSSLIKRLISYMKPFKLKITGVIILMII
                     VMGINLVNPYLLKEAIDKHVTNKNYNGLLVIGIFFSVLNVLSMFLSRIRINIMSEVSN
                     KIVLNIRQELYTHIQKLSFTFFDNRPVGKILARVVGDVNSLQQLFSDSVTNLIPEILT
                     IICTLFLMFSMNFKLTLAAVATLPLLIGGLFFIETFSRKRWQTYRKKRSNLNAFIHED
                     FSGIKVVQSYNMQEKTSYSFKKYCNSMMSSFIKAVRLNDFFWPMVELCSGIGIVLVYW
                     SSIKLVNTGSISIGTLVAFTAYISIFWRPIMNITNFYNSLITNFAAAERIFDILDTKP
                     DIENKGSEVIMPLINGNVQFKNVSFGYDDGTTVLDDVSFNVKPGEKIALVGPTGAGKT
                     TIINLISRFYDTKNGTVLIDGYDVKNVELESLRSQMGIMLQDTFLFSMSIMDNIRYGR
                     LDASDEEVINAAKAVNAHDFIMKLEKGYETNVNERGSRLSVGQRQLISFARALLADPR
                     ILILDEATSNIDTQTERLVQNGIKKLLYGRTSFVIAHRLSTIRDADRIFYVNDGKIVE
                     SGTHTELLAKKSAYYDLYMSQYKFISQGA"
     gene            complement(4442..5710)
                     /locus_tag="PLU28_06615"
     CDS             complement(4442..5710)
                     /locus_tag="PLU28_06615"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC45 family MFS transporter"
                     /protein_id="HOG34770.1"
                     /translation="MEFKYWKLFVLGFGFFGISIIWPLYNAYVPIFLKDFNLSSFMVG
                     VVMTFDNIFAIIMLPLIGAMSDKTRSKFGRRIPYILVGAPIATVFFIMIPFVKNMSIL
                     WLMMLAIIFMNFSMALFRAPVVALMPDITPTKHRSQANGIINFMGGLGALFAYFAGKP
                     MYDANPSFPFLVGGFVMLIASFIVAFTVKEPQEYTVVTGEESKKGTAKKGLLDLISNL
                     KDVFLSKEKSLLFMLFSILFWFIGFNAIETFFTSYMKFYVGVGESTGALILGFFSLTL
                     MLFSIPAGFIGSKIGRKKTIIIGLSVIIIMSVSTLVLGALIKNKIFDTQAIIAIFFAL
                     FAAGGVGWAMVNVNSLPMVVDMTTTDKAGGYTGLYYFFSMAANIIAPPLSGKIIDLFD
                     YNSLMFICLIFFGLAVFTITRVKRGEVQQK"
     gene            5882..6670
                     /locus_tag="PLU28_06620"
     CDS             5882..6670
                     /locus_tag="PLU28_06620"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOG34771.1"
                     /translation="MSENLNEFKSILTKVYLSDPCSVLPNPLWKTFGHLTDIQTDIKS
                     KGDNVTKLQAFNQREFMVYWSCFYENPDISEAEKKEFRLVVINQKYENFFDKLSFPIV
                     MRYFRLQHSGTELPAALSSQYYYEDVDIEKDCHMVSDFIGKCYENIKPSEKRVKSWTE
                     RGVFDNSLWLWIKEKGSGTPVALGIAEKDVINGEGSLEYIQVDPAFRGKGICKTLVNE
                     LVHRLYTKISIITVSGDLYNISNCEAVYRKCNFEGDSVWSVLKK"
     gene            6649..7323
                     /locus_tag="PLU28_06625"
     CDS             6649..7323
                     /locus_tag="PLU28_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34772.1"
                     /translation="MVCLKKIIIFFSFFISSIIFSFDLPYSHLEYKETIDSLSYKNII
                     DIRTEDNSICLDFSCPGKKYIVICNEELMTTYSFLKNTEKDSFYEIVLENRTIHITSF
                     QNGYESQSEYLWDGNTPWIQAIELSMVNFIKSDSTSMRFWLINPSDGKKMDMIAIKEG
                     IYQLDVLGKKENCTKVKVTLDSFLSIFWSVSYWFRNADGLFVRYEGLKGGPASPKKHI
                     SILNYN"
BASE COUNT         2535 a         1214 c         1329 g         2281 t
ORIGIN      
        1 ttgagatttt ttgtcttgtt ttaatttacg ttttgtaaat gaaaaagact tttttttgta
       61 attttgttac tatatacttt aaaacgtatt aaaaaaaata aaaggaggta aggccatgat
      121 aagaagaaag ttcagcgcag ttaacggcat ttttaatctt agcgggattg gtgtgtcaat
      181 tttgaatagc cttgcctctg tatcagcttc ttaaccgaag atcgataatt acagagccat
      241 ggttattcat ataccatggc tctttttata tgagagtaat taaaaaaaat tttttgatta
      301 atttttttta atagccatgg tataacgata ccatggtttt tttatttaag gaggtaaggt
      361 atgaataagc ttattcgggg aatagcagaa aactgaagtt gtggaactgg catcaaagta
      421 acatggcatt taagggagag ggcactttat gatgaaaatt ttcaatgaga tttttaatgg
      481 ttcaagaagt tacaggacag agtcacttca aaagagcaac tttacatttg agcttttaca
      541 aaactctatg ttgtcaaaaa ctaatgaaca tgaagaacta tatctaagga ggcatactta
      601 tgaaaaataa aaaatacttt atacaggaaa atatccagag taaaaatggc agatactcag
      661 ccgcactcga aaatatctat gagttaagtg aaatatacat tgaactatct aaaataattt
      721 aaaaaggaga taattatgaa aaatactatt aaaaaacaga gaaaatcaat agagtctttt
      781 gaagaaaaca aggaaactta tttgataaac aatattttat taagcgtacg ccgtttcatg
      841 taaaaatcag gcaaaggagt gtacctcatg aaaaaaatac ttaaatatat aggaaagtat
      901 aagctgttag tttttttacc tttcgttttt ctttttgtat caattgtttt agatcttatg
      961 cagccttttc tttcaaaggt tattgttgac agagtacttt tgaacaagga aacttccctg
     1021 cttggcatta tccttgtttc acttgctaca attactttag ttagggttat tctgggatac
     1081 tttcgggaat atacccttga ctaccttgga gtacgcgtgg caacagatat taaaaacgat
     1141 ctttttgatc acattcagac tctttctttc aaatactttg acaatatgaa cactggagag
     1201 cttatgtcaa gattgactga agatgtggat aatatatgga tggctttggc ttttggaata
     1261 agtttgttta ttgaaaattc aatatatttt atttttgctg ctgcaattct tttgtatctt
     1321 aactggaagc ttgctcttat aagcttgtgt atcatgccta ttctggcatg gctggctatg
     1381 aagcttgaga aagatataga aaaagctttt gataagataa gcgatcatgc cgtacatata
     1441 aacaccaccg cacaggagga tatttctggc atacggcttg taaaagcttt tgcacgcgaa
     1501 aagcatgaga ttctgaaatt tctgaatctg aatgcagtca actataattt aaacgttgaa
     1561 aaggcaaaga tctggggaaa gtactatcct ttgattgagt tccttacgaa tatctctata
     1621 gtcttagtca tttcttttgg aggaatgctg gttataaaag aagatatatc tatcggaact
     1681 cttgtggctt ttaacggata tctttccatg cttgtgtggc caatgagaat gatgggctgg
     1741 cttaccaacg tcatttcgca ggcaaatacc tcagctaaaa aaatctttgg tatcatgaac
     1801 acaaaagcag atatagcaag cacgcaagac tgtataaaac cattagaaat aaaaggagac
     1861 atcaccttta aaaatgtatc tttcaaatat aataactctt atgttttgaa aaatataaac
     1921 cttaatatta aagctggatc aaccgttgcc atcatgggga cgactggttc gggaaagact
     1981 tccctggtaa gtcttatcgg aagatactac gatgtgaccg agggatcagt tgaaattgat
     2041 ggtaaagaca tacgtgacct tgatcttgat cttttaagaa gttcaatgtc ggttgttttt
     2101 caagatacct tccttttttc tgattcaata gccaacaatg ttgctttttc agtaaaaaaa
     2161 tcttctgaac agagtataaa agacgcctgt aatatggcat gcatagatga tttcatctca
     2221 agtcttgaaa atggttatga tactgaaata ggagaaagag gaataggtct ttcaggaggt
     2281 cagaagcaac gtctttcaat tgccagggca atgattaaaa agtcatctat tttaatactc
     2341 gatgattcta catccgcttt ggatatggaa acggagtttc aacttcttaa gaatctgaac
     2401 ggtttgaaaa acaaacctac tacctttttg attgcacaca gaatctcggc tgttaaaaat
     2461 gccgatataa tcttgtacct tgaagatggc agtataaagg aatacgggac acataactct
     2521 cttcttgcaa tgaaaggcag atactatcag gtatactctg atcagttcaa agattacgaa
     2581 gagcttgaat acacagagga attagcataa cggaggtaaa taatgccaag aaatactaca
     2641 tctaaagatg aagatacttt aaaaacttct aaatcatcgt tgattaaacg tcttatctct
     2701 tatatgaaac ccttcaaact taagataacc ggggtaataa tcttgatgat tattgttatg
     2761 ggaataaact tagtaaaccc ttatcttttg aaagaagcta ttgataagca tgtgacaaat
     2821 aaaaattata acggacttct tgtaatagga attttctttt cagtccttaa tgttttatcg
     2881 atgtttttat ccagaattag aataaatatc atgtcggaag tatcaaataa aatcgtgctg
     2941 aatattcggc aggagcttta tacccatatt caaaagcttt cttttacttt ttttgataat
     3001 aggcccgtag gaaagatact agcaagagta gtcggagatg tgaactcact tcagcagttg
     3061 ttttcagata gtgttaccaa tcttatacca gaaatactta ctataatctg tactcttttc
     3121 ttgatgtttt ctatgaactt caaactaaca ctggcagccg tagcaactct acctcttctt
     3181 ataggcggat tatttttcat agagactttc agcagaaaga gatggcagac ctacagaaag
     3241 aaaagatcaa accttaatgc ctttatacat gaggattttt caggaattaa agttgttcag
     3301 tcctataata tgcaggaaaa aacctcttac agcttcaaaa aatattgtaa ttctatgatg
     3361 agttctttta taaaggcagt acggttaaat gactttttct ggcctatggt agaactgtgc
     3421 tccggaatag gcattgtctt ggtatattgg tcaagcataa agcttgtaaa taccggttct
     3481 atatcaatag gaacccttgt ggcttttact gcatatattt caattttctg gcgaccgatc
     3541 atgaatataa ccaatttcta taattcgtta ataacaaatt tcgccgcagc agaaagaatc
     3601 tttgatattc ttgatacaaa gcctgatatt gaaaataaag gctcagaagt aataatgcca
     3661 cttataaatg gaaatgtcca gtttaaaaat gtatcttttg gttatgatga cggaactaca
     3721 gtccttgatg atgtaagctt taacgttaag ccgggagaaa aaattgccct agttggtcct
     3781 accggagcag gtaaaactac tataataaat ctgataagca gattctacga tactaaaaac
     3841 ggaaccgttt taattgacgg atatgacgtt aaaaatgtag aacttgaatc tttacgatca
     3901 cagatgggta taatgcttca ggatactttt cttttttcta tgtctataat ggataatata
     3961 cgctacggaa gacttgatgc gagcgatgaa gaagttatca acgcagcgaa agccgtcaat
     4021 gctcatgatt ttatcatgaa actggaaaaa ggatatgaaa ccaatgtcaa cgaaagaggt
     4081 tcaaggcttt cagtaggaca gcgtcagtta atatcttttg caagagccct gctggcagat
     4141 cccagaatcc ttatcctaga cgaggctacg tccaatatag acacccagac agagcgtctt
     4201 gttcaaaacg gtataaaaaa acttctttac ggaagaacct cttttgtaat tgctcaccgc
     4261 ttatctacaa taagagatgc cgacagaatc ttttacgtca atgacggaaa aatagttgaa
     4321 agcggcacac acacagaact actagcaaaa aaaagcgctt attacgatct ttatatgtca
     4381 cagtacaaat ttataagtca aggcgcctga tacaaaaaaa taatcgtgcc ctagggcacg
     4441 attatttttg ctggacctcc ccgcgtttta ctctggttat tgtaaatacg gcaagtccaa
     4501 aaaatataag acatataaac attagtgaat tatagtcgaa caaatctatt atttttccgg
     4561 aaagaggcgg tgcaataata tttgctgcca ttgagaaaaa ataatacaat ccggtataac
     4621 ctccggcctt atctgttgtt gtcatatcaa caaccatagg aagagagttt acgttaacca
     4681 ttgcccaacc tactcctccg gcagcaaaaa gagcaaagaa aatagcaatt atggcctgag
     4741 tatcaaaaat cttgtttttt ataagagcac ccagaacaag tgttgaaact gacattataa
     4801 taataacgga taatcctatt attatagttt tctttctccc gatttttgag ccgataaatc
     4861 cagcaggaat agagaaaagc atcaatgtaa gtgaaaaaaa gcccagtata agtgcacctg
     4921 ttgattctcc tactcctacg taaaacttca tataactcgt aaaaaaagtc tctatggcat
     4981 taaaccctat aaaccaaaat agaattgaaa aaagcataaa aagaagactt ttttcctttg
     5041 aaagaaaaac atcctttaga ttggaaatca gatcaagcag tccttttttt gcagtacctt
     5101 ttttagattc ttcacctgta acaaccgtat actcctgcgg ttcttttacg gtaaaagcaa
     5161 ctataaagct ggcaataagc ataacaaatc ctccgaccaa aaagggaaaa gatgggtttg
     5221 catcgtacat tggcttacca gcaaagtaag caaaaagtgc accgagtcca cccataaaat
     5281 ttattattcc attggcctgg cttctgtgtt tggtaggagt tatatccggc ataagagcta
     5341 ctactggagc acggaacaat gccattgaaa agttcataaa tattattgca agcatcataa
     5401 gccaaagaat gctcatattt ttaacaaacg gtatcatgat aaaaaacact gttgcaatag
     5461 gtgctccgac aagaatatag ggaatacgcc ttccgaactt tgatcttgtc ttgtcactca
     5521 tagcgcctat caaaggaagc attattatgg caaatatgtt atcaaaagtc atgacaaccc
     5581 caaccataaa tgatgaaagg ttaaaatcct taagaaagat aggaacataa gcattataaa
     5641 gcggccagat aatgcttata ccaaaaaatc caaaacccag aacaaataac ttccagtact
     5701 taaactccat agaacgcccc ccttttagaa gtatttacag atagattata tcataatact
     5761 ctcttaatac tttattttta taaagtatac caaaaatgta caaattttct tttttatttt
     5821 tttatttgtg atattattgt ctgtgacgga ttctaaaatt attttttatt caggagaacg
     5881 tatgtctgaa aatcttaacg agtttaaaag tatacttaca aaagtatatc tttctgatcc
     5941 ttgctccgtg cttcccaatc ccctctggaa aacttttggt catcttacgg atattcagac
     6001 cgatataaaa tcaaaaggtg acaatgtcac aaagcttcag gcgtttaatc agagagagtt
     6061 tatggtttat tggagctgct tttatgaaaa ccctgatata agcgaagcag aaaaaaaaga
     6121 gttccggctt gtggttataa accaaaaata tgagaatttt tttgataaac taagctttcc
     6181 aatcgttatg aggtactttc ggctgcaaca ttctggaact gagcttcctg ctgctctttc
     6241 ttcacagtat tactatgaag atgtggatat tgaaaaagac tgtcatatgg tatcagattt
     6301 tattggaaaa tgctatgaaa acattaaacc ttcagaaaaa agagtaaaaa gctggacaga
     6361 aagaggagtt tttgacaaca gtttatggct gtggataaaa gaaaaaggaa gcggaactcc
     6421 tgtagcctta ggcatcgcag aaaaagatgt tataaacggg gaaggcagtc ttgagtacat
     6481 tcaggttgat cccgccttca gaggaaaggg tatctgtaag acccttgtaa atgaattagt
     6541 acatcggctt tatacaaaaa taagcataat aaccgtttcc ggtgatcttt acaatatttc
     6601 taactgtgag gcggtttata gaaaatgcaa cttcgaagga gatagtgtat ggtctgtctt
     6661 aaaaaaataa taatattttt ttcttttttc atttcaagca taatattttc atttgatctc
     6721 ccttattctc atcttgaata taaagaaacc atagattctt tatcatacaa aaacattatt
     6781 gatataagga ctgaagataa cagtatttgt cttgatttca gctgtccagg caaaaagtat
     6841 attgtaatct gcaacgaaga actcatgact acttactctt ttttaaaaaa taccgagaaa
     6901 gattcttttt atgagatagt tctagaaaac cgtacaatac atataacttc tttccagaat
     6961 ggatatgaaa gccaaagtga gtatctatgg gatggtaaca caccttggat tcaggccata
     7021 gagctgtcta tggtaaattt cataaagagc gatagcactt ctatgaggtt ttggcttata
     7081 aacccttcgg acggaaagaa gatggatatg attgctataa aagaaggaat ctatcagctt
     7141 gatgtattag gaaaaaaaga aaactgtacc aaagtgaaag ttactcttga tagttttctt
     7201 agcatcttct ggagtgtaag ctactggttc agaaatgccg acggtctttt tgtccgttat
     7261 gaaggactta aaggcggacc ggcgagtcca aaaaaacata tatctatcct taattataat
     7321 tagaggtgag tcttttgaag tataactcca aagtggata
//
LOCUS       DAOSHR010000102         7346 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_8761_length_7346_cov_4.418461, whole genome shotgun sequence.
ACCESSION   DAOSHR010000102 DAOSHR010000000
VERSION     DAOSHR010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 7346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7346
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8761_length_7346_cov_4.418461"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            118..741
                     /locus_tag="PLU28_06630"
     CDS             118..741
                     /locus_tag="PLU28_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34773.1"
                     /translation="MNELIDSLVNQLDSLPVIVAYTFFYLSGVLQMIFPPYPGDEIFI
                     FGGYLGSQGKVGGFLPIFISYFMGILSISFFFFLVSYKNKDQIYKNKIVGSVINEEVK
                     EKSRKNFKKHGWLMFFMGRFIPGLSLVTVIMGGVIGFNKSVALSLIAFSSFLHETVFF
                     FIGCVIGSNKLYITKFISIYNIFFIGILVVMVLLFVAKKIIKKEKRN"
     gene            753..1880
                     /locus_tag="PLU28_06635"
     CDS             753..1880
                     /locus_tag="PLU28_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34774.1"
                     /translation="MKLYISDTHIGENSDRIYDEKLLELFKKTDDRLQEVVIVGDFLE
                     LIDSTCIEADSWDVQEHIKLLDKIYSVHSELFNRIKNLSEKIEISYVYGNHDYMVGLC
                     PEVSSKIRQMMGNINILPYHYDEKTKVFAIHGNQFDVFNRFSLDESGKLVNPVGERLI
                     NYIAHGFISEMSQVVPEEILRDYVDVYPSIDVPKWFSYLSKVYRCNIKMKKIFVNTFV
                     DFCNSQELKQWLDINYPDSRALLKIAVNKYGGTAVSNLLARMILAIKRIKNSNYLMKK
                     SESLLCKEFLIDEKYVFGYGNKAVKFNTSQVQGIIMGHNHLPALKDYNGKFYANTGSW
                     KDILIFDKKNENKFIEKKRISYIEIENTKYSLEKKLVLEEI"
     gene            1918..2616
                     /locus_tag="PLU28_06640"
     CDS             1918..2616
                     /locus_tag="PLU28_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOG34775.1"
                     /translation="MSEDRILVVDDDDEIRSLVEIYLKNDGFIVCKAADGLQALQMIE
                     KEKYDLIILDLMMPGIDGIECCMRIRQKHDMPVIMLTAKNQDMDKITGLSMGADDYMT
                     KPFNPLELLARVKSQIRRYRGFSKAYQKKDNQRIEIGELAIDEGMHTAFVGDKEIRLT
                     KTEFEILFFLAKNRGMVFSAEKIYESLWQEESFDSENTVMVHIRKIREKIEVNPRNPQ
                     YIKTVWGVGYKIEK"
     gene            2606..4192
                     /locus_tag="PLU28_06645"
     CDS             2606..4192
                     /locus_tag="PLU28_06645"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOG34776.1"
                     /translation="MKNNFLKKLSAGVKRNIRIQIIIVFAVSIAIGGILAVFCEYILS
                     GNFNYGYEISEKITSEVASQIEEIDEKTGVNEIQEEIKEVSEKWESYFLKVYILNYEN
                     NIVYRYDPSGELEDTFPPGLFSIVQNSGHYTYDTYFLKFSQNGMIKLTDAYYNEKEST
                     DEEDTEFPKDGALPKVVTSVSGKGIYNSADIILMGERYILVTNIKVENPFGFLSVLSP
                     LIQLGFFIFGFIVSFIYLTQGKLKYIQDISKGLQAISKGDFEYRVPQKGDDEIGLLAK
                     NINSMADELKKSIYEERRSEKLKNELITNVSHDLRTPLTSVMGYLRLYTEKKYDSAEQ
                     AQEYIDIAYKKSISLKELIDDLFEYTKLNNFDIKLDMEVFDINRLARQVIQEIMPVAL
                     EKNIKFETNLKDQKFFINADAGKMARVVENIISNAIKYSGSGSRIDVVSFKRDCEVIL
                     EISNPSDSINDEDLKNIFERFYRIDKSRSGSLGGSGLGLSIAKSIIHLHEGKIWAEYK
                     DSKVSIFICMKLSKESSQCS"
     gene            complement(4163..6415)
                     /locus_tag="PLU28_06650"
     CDS             complement(4163..6415)
                     /locus_tag="PLU28_06650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG34777.1"
                     /translation="MPTLLKEKYFFSSEYISSEWGQIYTNSDSHITSYLDLQNKKIVY
                     IKDSYFFDSPAGILNILKSFRIDAIFLSVDTFQQAFDLVSNNLADAAVATRYQGYFFR
                     NTYKIKETAMMFSPIELRYAAKKNDSYAIKVINYIDSQMHLLKSSPDSPYYASIKKHL
                     PKEIAASDLPLWVVIIITLSILAIAVLFLFWIILKKQVKVKTRELEQSNEKLYQNYQE
                     LESLNEELSSTEEELLISHNRVIASEKRYRSIFNQAPVAMMTLDKTLHVTDYNMGAEK
                     IFGWKKEEIIGVVFYKYIFTEHDITFFDNFLADKSVKIPEYFVTENMNKSGDVIICEW
                     INSVIEDQKGNIIEIVSISRNITENKKLEESLRKAKDSAEKASKAKSEFLANMSHEIR
                     TPINGVIGISDLILETELDEEQKNYMQMIKTSSRNLLRIVNDILDISKIEAGKIELNN
                     VSFHVLELKENIMNSFFHLMKEKGLDFKFFIQENIPTNLNGDLYKINQILSNLVGNAY
                     KFTDKGYVHVKTEKVHEDSSSVELLFNVTDTGIGIKPEKINIIFDIFTQGDSSATKKY
                     QGTGLGLTIAKSYVEKMGGKIFVMSTPGEGSSFSFIIPIKKDISFAAEKPPEDEDTYL
                     HDYKDKKILVAEDNQINLYLTKKILSKKAMEVLTAENGAKAVEIFEKERPDLIFMDLQ
                     MPELDGLGATLKIRQIEKNHNTYTPIIALTAYATKEDKERCLAQGMDGFIAKPFTQKD
                     ILGALRTFLA"
     gene            complement(6309..6668)
                     /locus_tag="PLU28_06655"
     CDS             complement(6309..6668)
                     /locus_tag="PLU28_06655"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOG34778.1"
                     /translation="MRKTLFISLILFLSVFIYAQKVLIGVYENYPIVYKTSGNELQGI
                     LIDCFKIISEKSDYDFDFVYKTWPELLGDFENGTLNIITGIAYTSQREVFFFFRVHIF
                     RMGSDLYKLRLSHNFLS"
     gene            complement(6682..>7346)
                     /locus_tag="PLU28_06660"
     CDS             complement(6682..>7346)
                     /locus_tag="PLU28_06660"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HOG34779.1"
                     /translation="LDNIRPGIMEYELEAYFDFTLKSSGVKDFAFKTIAASGKNATVL
                     HYSQNNSMMNENDLILFDLGAQFEYYNADISRTFPISGKYSQRQKDVYDVVLQAQQAV
                     TQAAKPGVPFSILNDTAKKVLTEGCRKLGLIKEDNELSEYYYHGVSHYLGLDTHDVGT
                     RDTVLKEGMVITNEPGLYIASEGIGIRIEDDLLISHDGAEVLSKDILKDTFDVEAYIK
                     NR"
BASE COUNT         2379 a         1155 c         1391 g         2421 t
ORIGIN      
        1 taattctact ttatattata tcatagcact tttgagtaat taagaaataa ttaagaatta
       61 tctcagggat ttattaagca agccatcgta gaattctttt tgaagaggag gtaatatatg
      121 aacgaactca tagactcact tgtcaatcag cttgactctt tgcctgtaat agttgcatat
      181 acattttttt atctttcagg tgtcctacag atgatctttc caccatatcc gggagatgaa
      241 atatttattt tcggaggata tcttggttct cagggtaagg tcggtgggtt tttaccgatc
      301 tttataagtt attttatggg aatcctttca ataagttttt tcttctttct ggtaagttat
      361 aagaataaag atcagattta taaaaacaag attgtaggat ctgtaataaa tgaagaggta
      421 aaagagaagt cgcgtaagaa ctttaaaaaa catggatggt tgatgttttt tatgggaagg
      481 tttattccag gtctaagtct tgttactgtt attatgggag gtgtaatagg ttttaacaaa
      541 tcagttgctc tctctcttat tgcgttttct tcatttctgc atgaaacggt attttttttc
      601 attggatgtg taataggaag caataagctt tacattacca aatttatctc aatatataat
      661 atatttttca taggcatact tgtggtgatg gtgcttttat ttgtagccaa aaaaattatt
      721 aaaaaggaaa agaggaatta aggagaaaag atatgaagct ttatatttct gatactcata
      781 ttggtgaaaa ctcagacagg atatacgatg aaaagctgct tgagcttttt aagaaaactg
      841 atgataggtt gcaggaagtt gtgatagtcg gtgatttcct tgaactcata gacagcactt
      901 gtattgaagc tgacagctgg gacgtacagg agcatataaa gctccttgat aagatatatt
      961 cagtacattc agaacttttt aacagaatta aaaatctttc tgaaaagatt gagataagct
     1021 atgtttatgg gaaccatgat tacatggtag gactgtgtcc ggaggtatct tcaaaaatca
     1081 ggcagatgat gggaaatata aatattctgc cttatcatta tgatgaaaag acaaaagtat
     1141 ttgctattca cggaaatcag tttgatgttt tcaaccgttt ttcattggat gaaagtggaa
     1201 aacttgttaa tcctgtcggt gaaagactta tcaattatat tgcgcatggt tttataagcg
     1261 agatgagtca ggttgtgcca gaagagattc tcagagatta tgtagatgtt tatccttcaa
     1321 ttgatgttcc aaaatggttt tcatatctat ccaaagtata taggtgtaat ataaagatga
     1381 aaaaaatttt cgtaaacaca tttgtagatt tctgtaattc tcaggaactt aagcagtggc
     1441 ttgatattaa ttaccctgat tcaagggcat tactaaagat agccgttaat aaatatggag
     1501 gaacagctgt ttcaaatctt cttgcaagaa tgattctggc gataaaaaga atcaaaaact
     1561 ccaactatct gatgaaaaaa tccgaaagtc ttctatgcaa agaatttttg attgatgaaa
     1621 agtatgtttt cggatatgga aataaagcag taaagtttaa tacatcccag gtacaaggca
     1681 ttataatggg tcataatcat cttcctgccc ttaaagatta taacggaaag ttttatgcca
     1741 ataccggttc atggaaagac atacttatat ttgataaaaa aaacgaaaat aagtttatag
     1801 aaaagaagag aatctcttat atcgaaattg aaaatacgaa atacagtctt gaaaaaaaac
     1861 tagtgcttga agagatataa agatataaga tataatatca tcaggtaggt gataattatt
     1921 tcggaagatc gtattcttgt agttgatgat gatgatgaaa taagaagtct tgtagagatt
     1981 tacttgaaaa atgacggttt catagtctgt aaagcagccg atgggctgca ggctcttcag
     2041 atgatagaaa aagaaaagta tgatcttatc atacttgatc ttatgatgcc tggaattgat
     2101 ggcatagaat gttgtatgcg cataaggcag aagcacgata tgcctgttat tatgcttact
     2161 gctaagaatc aggatatgga taagataaca ggactaagca tgggagcaga tgattatatg
     2221 actaagcctt ttaatcctct tgaacttctt gcaagagtaa aatctcagat aagacggtac
     2281 agggggtttt ctaaagctta tcaaaaaaaa gataatcagc gcatagaaat aggcgaactt
     2341 gcaatagatg aaggcatgca cactgctttt gttggtgata aagagatcag acttactaaa
     2401 acagagtttg aaatactttt tttcctggca aagaataggg ggatggtttt tagcgcagaa
     2461 aaaatatacg aaagtttgtg gcaggaagaa agctttgatt cggaaaatac cgttatggtt
     2521 catatcagaa agatacgtga aaaaattgag gttaatccca gaaatcccca gtatataaaa
     2581 acagtatggg gagtaggtta taaaattgaa aaataatttt ttaaaaaaac tgtcggcagg
     2641 cgtaaaaagg aatataagaa tccaaataat aatagttttt gcagtaagta tagccattgg
     2701 aggcatactt gctgtttttt gtgagtatat actctccgga aactttaact atggatatga
     2761 aatttcagaa aaaataacct cggaagtggc ttcacagata gaagaaatcg atgaaaaaac
     2821 aggcgttaat gaaatacagg aagaaataaa agaagtatct gaaaaatggg aatcgtactt
     2881 tcttaaagta tatattctta attatgagaa taatatagta tacagatacg atccttcagg
     2941 cgaacttgaa gatacgttcc ctcctggtct tttcagtatt gtacagaaca gcggtcacta
     3001 tacttacgat acgtactttc ttaagttcag ccagaatgga atgataaagc ttaccgacgc
     3061 ctattacaat gaaaaggaaa gtacggatga agaagatacc gaatttccga aggatggagc
     3121 tttgcctaag gtggtaacct ctgtttcggg caaaggaata tataattccg cagatattat
     3181 tttaatggga gaaaggtaca tacttgttac caatattaaa gtcgaaaatc cttttggatt
     3241 tctttcggtg cttagtcctt taatacagtt gggctttttt atattcggat ttatagtctc
     3301 ttttatctat cttactcagg gaaagctcaa gtacatacag gatatctcca aaggacttca
     3361 ggcaatatcc aaaggtgatt ttgaatatag agtacctcag aagggtgacg atgaaatagg
     3421 cctgctggca aaaaatatta acagcatggc tgatgaatta aagaagtcaa tatatgaaga
     3481 gagaagatct gaaaagctaa agaacgagct tataacaaat gtatcgcatg atttaagaac
     3541 gccgcttacc tctgtcatgg gatatcttag gctttataca gaaaaaaagt atgattctgc
     3601 cgaacaggca caggaatata tagatattgc atacaagaaa tctattagtc ttaaagagct
     3661 tatagacgat ttgtttgaat ataccaagct gaataatttt gatataaagc ttgatatgga
     3721 agtttttgat atcaacagat tggcaaggca ggttattcag gaaattatgc ctgttgccct
     3781 tgaaaaaaat ataaagtttg aaaccaatct caaagaccag aaatttttta ttaatgccga
     3841 tgccggaaag atggcacgag tagttgaaaa tataatatct aacgcaataa agtattctgg
     3901 tagcggcagc agaattgatg ttgtaagttt caagcgggac tgtgaggtta ttcttgaaat
     3961 ttctaatcca tcagatagca ttaatgatga agatcttaaa aatatatttg aaaggtttta
     4021 cagaatagat aaatcacgtt caggcagcct tggaggttca gggcttgggc tttcaatagc
     4081 caaaagcatt atacatctgc acgaagggaa aatatgggca gagtataaag atagtaaagt
     4141 atctattttt atatgtatga aattaagcaa ggaaagttct caatgctcct agtatatcct
     4201 tctgggtaaa aggttttgca ataaaaccat ccatgccttg agcaaggcaa cgttccttgt
     4261 cttccttggt agcgtatgct gtaagtgcaa ttatgggggt gtaagtgtta tgattttttt
     4321 cgatctggcg aatttttaaa gttgctccca atccgtcaag ttccggcatc tgaagatcca
     4381 taaatattag atccggacgc tctttttcaa aaatttcaac cgctttggct ccattttctg
     4441 ccgtaagcac ttccattgct ttttttgaaa gaattttctt tgtcagataa aggtttatct
     4501 gattatcttc agcgacaaga atttttttgt ctttatagtc atggagatat gtgtcttcat
     4561 cttccggagg tttttcagca gcaaagctaa tatctttttt tatgggaata atgaaggaga
     4621 aagaactgcc ttcgcccggt gtgctcataa caaatatctt tccgcccatc ttttcaacat
     4681 aacttttagc tattgtgagt cctaagcctg ttccctgata tttttttgtg gctgaagagt
     4741 cgccctgcgt aaagatatca aatataatat ttatcttttc cggttttata cctataccgg
     4801 tatcagttac gttaaacaaa agttcgaccg aagaactgtc ttcgtgtact ttttccgttt
     4861 tcacatgaac gtatcctttg tcagtgaact tataagcatt gcctacaaga tttgaaagta
     4921 tctggtttat cttgtaaaga tctccgttga ggttggtagg aatattttcc tgtataaaaa
     4981 atttaaaatc aagtcctttt tccttcataa gatggaaaaa tgagttcata atattttcct
     5041 ttaactcaag aacatgaaat gaaacattgt taagttctat ttttccagct tcaatctttg
     5101 aaatatcaag tatatcgttt actattctca aaagatttct ggatgaagtt tttatcatct
     5161 gcatgtagtt tttttgttct tcatcaagct ctgtttctag aatgagatcg cttattccta
     5221 tgactccgtt tataggagta cgtatttcgt ggctcatatt ggcaaggaac tcacttttgg
     5281 ctttgcttgc tttttccgca gagtcctttg cttttcttag tgattcttcc aattttttgt
     5341 tttcagtgat gtttcttgaa atagaaacta tttcaattat gtttcccttc tgatcttcaa
     5401 tgacagagtt tatccattcg catattatga catctcctga tttgttcatg ttttctgtga
     5461 caaagtattc aggtatttta acagacttat ctgcaaggaa attgtcaaaa aaagtgatat
     5521 catgttcggt aaaaatatat ttataaaaaa ccactcctat aatttcttcc ttcttccatc
     5581 cgaaaatttt ttctgctccc atattataat cagttacatg aagagttttg tccaaagtca
     5641 tcatggccac aggtgcctga ttgaaaatgc tccggtagcg tttttcactt gcaattactc
     5701 tattatggga tatcagcagt tcttcctcag tgcttgaaag ttcttcattc aaagactcca
     5761 gttcctggta gttttgataa agtttttcat tgctttgttc cagctctctt gttttaacct
     5821 taacctgttt tttaaggata atccagaata agaataaaac cgctatggcc agtatactta
     5881 aagtaattat gataactacc cacaaaggaa gatccgaagc agcaatttct ttcggcaaat
     5941 gtttttttat tgaggcgtaa tatggcgaat cgggagaact ttttagtagg tgcatctgcg
     6001 aatcgatgta attaataact tttatggcat aactgtcatt cttttttgca gcatatctaa
     6061 gctctattgg actgaacatc attgctgttt cttttatttt ataagtgttt cggaaaaaat
     6121 atccctgata acgtgtagct accgcagcat cagccaagtt attgcttaca agatcaaaag
     6181 cctgctgaaa agtatcaacg gaaagaaata ttgcatcaat cctaaaactt ttgagaatat
     6241 ttagaatacc tgcaggcgaa tcaaaaaagt aactgtcttt tatatataca atttttttgt
     6301 tctgaagatc aagataagaa gttatgtgag agtcggagtt tgtataaatc tgaccccatt
     6361 ctgaagatat gtactctgaa gaaaaaaaat acttctcttt gagaagtgta ggcaatacct
     6421 gttatgatgt tcaaggttcc gttttcaaaa tctccgagaa gttcaggcca tgttttatat
     6481 acaaaatcaa aatcataatc agacttctcg gaaattattt taaaacagtc tatcagaatt
     6541 ccctgtagct cgttaccaga agttttgtaa actatcggat agttttcata tacgcctata
     6601 agtaccttct gtgcatagat aaaaaccgac aaaaaaagga taagagaaat aaacagagtt
     6661 tttctcatat gtccccctta tctatctgtt ctttatatac gcctctacat caaaagtatc
     6721 ttttagaatg tcttttgaaa gaacctctgc tccgtcatga gatataagga gatcatcctc
     6781 gatacgtatc ccaatacctt cggaggctat ataaagtccg ggctcgttgg taataaccat
     6841 gccttctttc aagacagtat cgcgtgtacc gacatcatgt gtgtcaaggc caaggtagtg
     6901 agatactccg tgataataat actccgaaag ctcattatcc tcttttatca gtccgagctt
     6961 tctgcagcct tctgtcaaaa ctttttttgc cgtatcattc agtatagaaa aaggaactcc
     7021 tggttttgca gcctgagtta ctgcttgctg tgcctgaaga acaacatcgt aaacatcttt
     7081 ttgtctttga gagtactttc cgcttatagg aaatgttctg gatatatctg cattataata
     7141 ttcaaactgt gcaccgaggt caaagagtat aaggtcgttt tcgttcatca tagaattatt
     7201 ctgtgagtaa tgaagaactg ttgcattctt gccgctggcg gctatggtct tgaaagcgaa
     7261 atcttttacg ccgctgcttt ttaaggtaaa atcaaagtat gcttcaagct cgtactccat
     7321 tataccaggc cggatgttgt caagaa
//
LOCUS       DAOSHR010000103         7231 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_8970_length_7231_cov_5.597826, whole genome shotgun sequence.
ACCESSION   DAOSHR010000103 DAOSHR010000000
VERSION     DAOSHR010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 7231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7231
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8970_length_7231_cov_5.597826"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1407)
                     /locus_tag="PLU28_06665"
     CDS             complement(<1..1407)
                     /locus_tag="PLU28_06665"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOG34780.1"
                     /translation="MNLSKERYLEILNIALSQGGDYSEIFFEDYYATSILYDNGKFEG
                     SSYSKRNGASLRVVIGEETLFAHTSSPDYASLKKIAENLSSIAKTRSSSAEIKKVLDL
                     KELPKRNLIKIDKEFDKIPVEEKMEKINMAVKVAKEYDPRIIQITVRYGDSKRTVRIV
                     NSDGTIADDVRSYPLFYVSIVAKSEDGQIFTGMSGNSENKGFEFFDDNMIIQAAKDAC
                     GQAIAQVEGEDAPAGEFTVVMSSQAGGTMVHEACGHGMEADLVLSGSVYRDKAGQKIA
                     SEKVTVVDDGTIPGKRGTLNYDDEGSQTQRTVLIENGILKGYMHTKITAKMFGKEPTG
                     NGRRETYMNLPIVRMRNTLILPGNDNPDDIIKSVEKGVFVKKMGGGQVDVISGDFQFK
                     ITEGYNIENGKTTTSLRGASIVGNGLKVLQSIDMVGNDLGYAVGTCGKDGQGAPVSDA
                     QPTIRVPKMIVGGVVKGGK"
     gene            1582..3150
                     /locus_tag="PLU28_06670"
     CDS             1582..3150
                     /locus_tag="PLU28_06670"
                     /inference="COORDINATES: protein motif:HMM:NF017633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HOG34781.1"
                     /translation="MPIDGLAIRKLINEARERVVNKSIKNIYQPVEDQLLIQTSGDFL
                     LFSVQNPSYLLVIEKKPDIPENPYNFSMFLRKRIKGGKILSVEQLGTDRIGYMDIQGY
                     DEIGDLRKYRLYFELMGRNSNIILVNEDNVIQDAMKRGTAETRLLIHGIKYIPFFDDS
                     KVNIMKEDISDIKAASNLQGFCKTSQGSLMFYNTLQELRQEIISPYLYVYTDEKGNRD
                     LLALKPVGSDIKKLTPSAAIIDVFEFKANQSRFLAIRKELLKKLEREVDKDEKIKDNL
                     KKDISEQSKIPELSKKAELLQSFVYKVSKGDDSILTKDWLTGEDILIKLDPIKTPSQN
                     LQDYFRKLKKIKNRVGYALEKIVHIEKELEYYYQLMAMLDEAEDIQTLEEIKVEMIQQ
                     GLIDAKNKTVKKSEKNNPYRKFSYNGFDILVGKNNVQNDEITKNARADDIWLHTRGIP
                     GSHVIIKCAGKEVPYDVILYAATVAAKYSRAKFSSNVGVDYTKKSNVYKPKGSKPGMV
                     LYESYSTVFVTPVF"
     gene            3163..4425
                     /locus_tag="PLU28_06675"
     CDS             3163..4425
                     /locus_tag="PLU28_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOG34782.1"
                     /translation="MKIAVIGFGAASIGFLNTVKNTGHEVHIFESSKDIYSSSLSGIR
                     SDGKLFVSGSMGGDLLVDIKLQKEVVDFYINHSALGKNEIEYGYSFSNAELYKKFYDY
                     GFDPVKSDFYHIGTDKLGDILTAIYSEFKTFNNMHFYFGNRVESVEPAEYGAQIQVNG
                     EKILFDLAVVAAGRSGHKLTSKFIQEHPEVVLSNTTVDLGVRYELPDHIVQELNEEMY
                     EFKVKLKTKTGYMVRTFCNNPSGEVVLEKYDDFVTVNGHARMKEKTTNTNFAVLVTHS
                     FTSPFNDPVGYGSYIAKLSNILAGNNKIILQAYGDFKQAKRTKKLGRVLPTLKSDNYI
                     LGDLNLVFPRKTVESIIYFLEELNKVIPGITYEDNLLYGVEVKFYGNKLDNSKYKNIK
                     FIGDCSGWTRSITYATSHGIMLGQELFL"
     gene            4478..5125
                     /locus_tag="PLU28_06680"
     CDS             4478..5125
                     /locus_tag="PLU28_06680"
                     /inference="COORDINATES: protein motif:HMM:NF016382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease V"
                     /protein_id="HOG34783.1"
                     /translation="MKKIQYELASKVEIGGKIRTPSIIGGVDVSFMGEKAVGIVVCLA
                     FPSLELIEYSYSSVEVSMPYIPGYLAFRELPAFLKAWEKIKNVPDVVMFDGHGVAHPR
                     RLGIASHASFFIERPTIGIGKSLLYGITVEPAKEKFSQNPIYDNVYTKHPNDSIIGMS
                     MCSKEKTKPIYISCGNLISLEEAIKLSQMSITKYRLPEPTRYAHLYSQKVKKEIS"
     gene            complement(5118..6290)
                     /locus_tag="PLU28_06685"
     CDS             complement(5118..6290)
                     /locus_tag="PLU28_06685"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HOG34784.1"
                     /translation="MKKIFYVFLAAMIFLVSGCQNDSYYKTLYEKTIKDFGSFNKDKI
                     YKVLILNSYSIEHVFTVNEVRGILDSLRSNPNINIEISYLYMDTKTNFESTYLQELHN
                     LLMAKYADTVFDFIVVTDNEALEFAKLHKDGFFKSQNLIFCGISNFSPSLIEGLENIS
                     GVIEANNLERTLDIIPKIRPMTENIFVVTDQTITGSMYRKLLEDYISKQENGPKIMLL
                     DMGELKMAELLARICLIPNNDSLIILQNFVDKDGVIYSVNQAMKLFLECSRVPIFVSS
                     ETRLGMGGVGGNVASGFKQGQIAGQIGMRLLNGESLKNIPVVTEDTNIFSFDHNVLKK
                     WNIDEKLLPKDSVITNKITINSKDFLIFIIAAFAIFLAIYSIIMTLKLRKFRRKSN"
     gene            complement(6301..>7231)
                     /locus_tag="PLU28_06690"
     CDS             complement(6301..>7231)
                     /locus_tag="PLU28_06690"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HOG34785.1"
                     /translation="DATVLFYDFDTQQKNLSNIKYLESLIDDAKRYIYISIYDINHST
                     IADALKRAAQRGIEVKMVTETQNQNTFTSLSLPRNNIKVLFDTNIALMHDKFIVIDGY
                     CLVTGSTNLTNNCLEYNSNNMIFIFNNEIAENYVQEFKEQFEQKIFGKKAGTEKPFPS
                     VLTDIGTISTYFTPEDNLKQEILKNIKECTKNIYMMIFTFTDPDIAQELICASERGVE
                     VKVITEEFQSKASYAAYNILKGKIDIILDKNSKTFHHKVIIFDDNKVLTGSFNFTGAA
                     QDKNDENSLIIESNYINNVYTDEFMRLWDSYTK"
BASE COUNT         2266 a         1299 c         1353 g         2313 t
ORIGIN      
        1 tttacccccc tttacgactc ctcccacaat catttttggt actcttatag ttggctgggc
       61 atctgatacc ggggcgccct gaccatcttt gccgcatgtt cccactgcat atcctaagtc
      121 gtttccaacc atatctatgc tttgaagtac tttaagcccg tttccgacta tgcttgcacc
      181 acgcagggaa gtagttgttt ttccgttttc tatgttgtat ccctcggtta ttttaaactg
      241 gaaatcgccg ctgattacat ctacctgacc gcctcccatc ttttttacaa atactccttt
      301 ttctacagat tttataatat catccggatt atcatttcca gggaggataa gagtatttct
      361 cattctgact atcggcaggt tcatatatgt ttcacgtctt ccgtttcctg taggttcttt
      421 tccaaacatt ttagcggtta ttttggtgtg catatagccc ttaagaattc cgttttctat
      481 cagaacggtt ctctgtgtct gtgaaccttc atcgtcatag ttcagagttc ctcgtttacc
      541 aggaatagta ccgtcatcca ctactgtcac tttttctgaa gcaatcttct gaccagcttt
      601 atccctgtat acagaaccac tgagaacaag atctgcttcc attccgtgtc cacaggcttc
      661 atgaaccata gttcctccgg cttgactgga cattacaact gtaaattcac ctgccggagc
      721 atcctcgcct tcaacctgtg ctatggcttg tccgcatgca tctttggcag cctgtattat
      781 catgttgtcg tcaaagaatt caaaaccttt gttttcagag tttcctgaca tacctgtaaa
      841 aatctgtcca tcttcgcttt tagcaacaat tgaaacgtaa aaaagcggat agcttcttac
      901 atcgtcggca atagtgccgt ctgagttaac tattcttaca gttcttttgg aatctccata
      961 tcttacggtt atctggatta tccgtggatc atactctttg gctactttga cagccatatt
     1021 tatcttttcc attttttcct caacagggat tttatcgaac tccttatcta tttttatcag
     1081 atttcttttt ggaagttctt ttaaatcaag tacttttttt atttctgctg atgaggatct
     1141 tgttttagct atactgctca ggttttcagc aatttttttg agagaagcat aatcagggct
     1201 gcttgtatgg gcaaaaagtg tttcttcgcc tataactact cttaagctgg ctccatttct
     1261 ttttgaatag cttgaaccct caaactttcc gttatcgtat agtatagatg ttgcatagta
     1321 atcttcaaaa aatatttccg aataatctcc gccttgagaa agtgcaatgt ttaaaatctc
     1381 aaggtatctt tctttagaaa gattcacgtt tatccctcct gatttgataa aattatgctt
     1441 attatattcc attatatcat atatatacgt tatgaaacgc cggaacactt ttctcataat
     1501 aattatactt ctgttttttt gtaaaataat ttcaatatag atatcttact atgataaaat
     1561 taaaatgtga ggtgataatt tatgcctatt gatggacttg ctattcgtaa acttatcaat
     1621 gaagcaagag agcgtgttgt gaataaaagt attaaaaata tataccagcc tgtggaagat
     1681 cagcttctta tacagaccag cggtgatttt ctgctttttt ccgtgcagaa tccatcgtat
     1741 cttctggtaa ttgaaaaaaa acctgatata ccggaaaatc cttataattt ctcaatgttt
     1801 ctgaggaaaa gaataaaagg aggaaagatt ctttcggttg aacagttagg aacagacagg
     1861 ataggctata tggatattca gggttatgat gagataggag atctgagaaa gtacagactc
     1921 tactttgaac tcatggggag aaattcaaat attattcttg ttaacgagga taatgttatc
     1981 caagatgcta tgaaaagagg tactgcagaa acacgtctac ttatacacgg aataaagtat
     2041 ataccctttt ttgatgattc taaagtaaat ataatgaaag aggatatatc agatataaaa
     2101 gcagcttcaa accttcaggg attttgtaaa acttcacagg gctcactaat gttttacaac
     2161 actttacagg aattgagaca ggagataata agtccatatc tgtatgttta tacagatgaa
     2221 aaggggaaca gagatctttt ggcattaaag cctgtcggtt cggatataaa aaaacttacg
     2281 ccatctgctg ccattattga tgtttttgag ttcaaagcca accagtccag atttcttgcc
     2341 atacgtaagg aacttttaaa gaaattggag agggaagtag ataaagatga aaagataaaa
     2401 gataatctaa aaaaggatat ttccgaacag tcaaaaattc ctgaactatc gaaaaaagca
     2461 gagcttctac aaagctttgt ttataaagta agcaagggtg atgacagtat tttaaccaaa
     2521 gattggttaa ccggagaaga tatccttata aagcttgatc ctataaaaac accatcacag
     2581 aatctccagg attacttcag aaagcttaaa aaaataaaaa acagagtcgg atatgcactt
     2641 gaaaagatag tgcatataga aaaagaactt gagtattatt atcagcttat ggcaatgctt
     2701 gacgaggcag aggatataca gactcttgaa gaaataaaag tagagatgat tcagcagggg
     2761 ttgattgatg ccaaaaacaa aacagtcaag aaaagcgaaa aaaacaatcc gtacagaaaa
     2821 ttcagctata acggtttcga tatactggtt gggaaaaaca atgtgcagaa tgatgagata
     2881 accaaaaatg cgcgggcaga tgacatatgg ctccatacaa gaggaattcc cggatcacat
     2941 gtcataataa aatgtgccgg aaaagaagtt ccgtacgatg tcatacttta tgctgccaca
     3001 gttgcggcaa aatattccag ggccaaattt tcctcaaatg taggagttga ttataccaaa
     3061 aaatctaatg tttataaacc taaaggctca aaaccgggaa tggttttata cgaaagctac
     3121 tccacagtgt ttgttacacc tgttttttaa ggaggaaaga gtatgaaaat agcagtaata
     3181 ggctttggtg cggcatctat aggttttttg aacacggtta agaataccgg acatgaagtt
     3241 catatctttg aaagttccaa ggatatatat tcatcaagtc tgagcggtat caggtctgac
     3301 ggaaagcttt ttgtctctgg aagtatggga ggagacctgc tggtagatat aaaacttcaa
     3361 aaagaagttg tagacttcta tataaaccat tcagcactgg gaaaaaatga aatagagtac
     3421 ggttattcgt tttcaaatgc cgaactgtat aagaaattct atgattacgg gtttgatcct
     3481 gtgaagtcag acttttacca tataggaacg gataaactcg gagatattct tacagctata
     3541 tactcggaat ttaagacgtt caataatatg catttttatt ttgggaacag ggttgaaagt
     3601 gtagagccgg cggagtatgg agcacaaata caggtaaacg gagaaaaaat cctttttgat
     3661 ctggctgtgg ttgccgcagg gcgcagcgga cacaagctca cttcgaagtt tatacaggaa
     3721 catccggagg ttgtcctttc caataccacg gtagatcttg gtgtaagata tgaacttcca
     3781 gaccatatag tacaggaact gaacgaagaa atgtacgagt tcaaagtaaa gcttaagacc
     3841 aaaactggat atatggtcag aactttttgc aataaccctt ccggtgaggt tgttcttgaa
     3901 aaatatgatg attttgttac cgtgaacggt catgccagaa tgaaggaaaa aacaaccaat
     3961 accaactttg ctgtgttggt tacacactcc tttacctcgc ctttcaatga tcctgtagga
     4021 tatggctcat atattgcgaa gctttcgaat attcttgcag gcaataacaa gattatactt
     4081 caggcttatg gtgattttaa acaggccaaa agaacaaaaa aactaggaag ggtgcttccc
     4141 acgcttaaaa gcgacaatta tatacttggg gatctcaact tggttttccc aagaaaaacc
     4201 gtcgagtcta taatttactt tcttgaagaa ttaaataaag taataccggg tataacttat
     4261 gaagataatc ttctgtacgg agttgaagta aagttttatg gaaataagct tgataacagt
     4321 aagtataaaa atataaagtt cataggcgac tgctccggct ggacacggtc gataacgtat
     4381 gcgacatctc atgggataat gctcgggcaa gagctgttcc tataaaatta tggctgaata
     4441 taaacaagtc cattcctttg acctgcagat tagccagatg aagaaaattc agtatgaact
     4501 ggcttcaaaa gtggagattg gcggaaaaat aagaactccc tccataatcg gaggggtaga
     4561 tgtttctttt atgggagaaa aagcagttgg aatagttgtt tgtcttgcat ttccttcgtt
     4621 ggagcttatt gagtacagtt attcatctgt tgaggtctct atgccttata ttccgggata
     4681 ccttgcattt agggagcttc ctgcatttct aaaagcatgg gagaaaataa aaaatgtgcc
     4741 agatgtagtg atgttcgatg gtcatggtgt agcccatcca agaagattgg gaattgcaag
     4801 ccatgcttct ttttttattg aaagacctac tataggaata ggaaagtccc tcctttacgg
     4861 gataaccgtt gaacctgcaa aagaaaagtt ttcacagaat ccgatttacg ataacgtgta
     4921 tacaaaacat ccgaatgaca gtataatagg aatgagtatg tgctcaaagg agaaaaccaa
     4981 gcctatctat atatcctgtg gaaatctaat ctctttggaa gaggcaataa aactttcaca
     5041 gatgtccatt acaaagtacc gacttcctga accgacaaga tatgcacatc tatacagcca
     5101 gaaagtaaaa aaagagatca gttagatttt cttctgaatt ttctgagttt taaagtcatt
     5161 atgattgagt atattgcaag aaatattgca aatgctgcaa taatgaatat aagaaagtct
     5221 tttgaattaa tagtaatttt attggttatt accgaatcct tgggtaaaag tttctcatca
     5281 atattccatt ttttgagtac gttatgatcg aacgaaaaaa tattggtgtc ttctgtcacg
     5341 acagggatat tttttagtga ctctccgttt aaaagcctca ttcctatctg tccggcgatc
     5401 tgtccctgtt tgaaaccgct ggcaacattc ccacccacac cgcccattcc aagtctggtt
     5461 tcgcttgaga caaaaatcgg tactcttgag cattcaagaa aaagtttcat tgcttgattt
     5521 acagagtata ttactccgtc tttatctaca aaattttgaa gaattattaa agaatcattg
     5581 tttggaataa gacagattct ggcaagcagc tcggccattt tcagctctcc catatctaac
     5641 agcataatct tcggcccatt ttcctgcttt gaaatataat cctccaaaag ttttctgtac
     5701 atacttccgg tgattgtctg atcggtaacg acaaaaatat tttctgtcat cggacgtatt
     5761 ttcggtatta tatccagagt tctttcaagg ttattggcct ctatgacacc tgaaatattc
     5821 tcaagacctt caataaggga tggactaaag ttgcttatac cacagaatat aagattctgg
     5881 cttttaaaga acccgtcttt atgaagtttc gcgaattcca gagcttcatt atcggttacc
     5941 actataaaat caaaaacagt atcggcatac tttgccataa gaagattatg aagctcctgc
     6001 agataggtgc tttcaaaatt tgtcttggta tccatataca gataagagat ttctatgttt
     6061 atattcggat ttgaccttag tgaatccaga attcccctga cttcatttac agtaaaaaca
     6121 tgctctatgc tgtaagagtt taaaataagc actttataga ttttatcttt gttgaaagag
     6181 ccaaaatcct ttatggtttt ttcgtacaaa gtcttgtagt atgagtcatt ctgacatccg
     6241 gatactaaaa agatcattgc agcaaggaac acataaaaaa tttttttcat atcaactcac
     6301 ctatttggtg taactatccc atagtctcat gaattcgtct gtatagacat tattgatata
     6361 attgctttcg ataattaagg agttttcatc gttcttatcc tgagctgcac cggtaaagtt
     6421 gaaagatcct gtaagaactt tattatcgtc aaatataatg accttatggt gaaaggtttt
     6481 actgttttta tcaagaatga tatctatctt tccctttaaa atattatatg cagcataaga
     6541 agctttggac tgaaactctt ccgttataac ttttacttcg actccccgtt cagaggcaca
     6601 gataagttct tgggcaatat caggatcggt gaaggtaaag atcatcatat aaatattttt
     6661 ggtacattct ttgatgtttt taagaatttc ctgtttaaga ttgtcctcag gcgtaaagta
     6721 tgtgcttatg gttccgatat ctgtaagaac tgaaggaaat ggtttttcag tgcctgcttt
     6781 tttgccgaag attttttgct caaactgttc tttaaactcc tgtacataat tttcagcaat
     6841 ctcattattg aaaataaaga tcatattatt agagttatat tcaagacagt tattggttag
     6901 attggtactt cctgtgacaa gacaataccc atctattaca atgaacttat cgtgcataag
     6961 cgcgatgttt gtgtcaaaaa gcactttaat gttattacgt ggcagagaca gagaggtaaa
     7021 agtattttga ttttgagtct ctgtaaccat tttgacttct attcccctct gtgcagctcg
     7081 ttttaaggca tctgcaatcg tactgtggtt tatgtcatag atgctgatgt aaatatatct
     7141 tttagcatcg tcaatcagag attcaaggta ctttatattt gaaagattct tttgctgagt
     7201 gtcaaaatca taaaaaagaa cagttgcatc c
//
LOCUS       DAOSHR010000104         7203 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_9021_length_7203_cov_4.870593, whole genome shotgun sequence.
ACCESSION   DAOSHR010000104 DAOSHR010000000
VERSION     DAOSHR010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 7203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7203
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9021_length_7203_cov_4.870593"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="PLU28_06695"
     CDS             complement(<1..318)
                     /locus_tag="PLU28_06695"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOG34786.1"
                     /translation="MENISYRYILNRSDFKKDIFILHGLFEYPGVYEKMAQRLSDSGY
                     KAHMIGMQGFGNLYAAKEELDFYSIFEFLERTVPSDYVLFGSCLGGVISASFSIKTEK
                     KPSK"
     gene            complement(339..1595)
                     /gene="rho"
                     /locus_tag="PLU28_06700"
     CDS             complement(339..1595)
                     /gene="rho"
                     /locus_tag="PLU28_06700"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOG34787.1"
                     /translation="MDMAKLNDMTRTQLYALARNFEITNYSKMSDHELIFSILRKQTE
                     SIGYFFHEGILEIFSDGYGFLRSIDNSLLQGSDDIYVSNTQIKRFNLFTGDVIAGQVR
                     PPKDGEKYFALLRIEAINYQAPEKARDRISFENLTPEYPDRQMRLENKSGPISARIID
                     MISPIGFGQRTLIVAPPKAGKTTLLKDTANSIAKNFPNTKRFVLLIDERPEEVTDLKD
                     TVNANVIAAPFDMDPKDQIKVAEMTLHLAKKLVEFGHDVVILMDSLTRLARAYNLVVP
                     SSGKLLSGGVDPAALSFPKKFFGAARKIREGGSLTIIATALVDTGSKMDELIFEEFKG
                     TGNMELILSRELSDKRIFPAINLKLSGTRKEELLLSEKNMKNMFILRQFINDVNVEEA
                     LTFFMNLMKKHTENQDLYEAVEKQKI"
     gene            complement(1977..2366)
                     /gene="rplQ"
                     /locus_tag="PLU28_06705"
     CDS             complement(1977..2366)
                     /gene="rplQ"
                     /locus_tag="PLU28_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HOG34788.1"
                     /translation="MRHRVKMNKLSRPASHRNALVKNLARNVFESGSIITTSAKAKVA
                     QSLVERVLSKAKEASTTADASRKMALSRDINRNFNDRKLTAKIVNEIAPKYSNKVSGF
                     TRVLKIGFRRGDAAELSLLQLIPTEEK"
     gene            complement(2363..3424)
                     /locus_tag="PLU28_06710"
     CDS             complement(2363..3424)
                     /locus_tag="PLU28_06710"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOG34789.1"
                     /translation="MELLIKPEVFQLEEERLAEEGIRYAKYVLYPLEKGYAITIGNAL
                     RRVLLSSIPAVAITSIRIPNKLHEFDTIEGVKEDILEIALNLKKVQLKVDDLKKLNSI
                     EGELHLKINKQGPSDITAGDIEVPSEISIANPDFKIATINGKNKVEIELFAQIGKGFV
                     PASEMEHENDIEFIYIDGVFSPVLKVNYLTENIRVGKRTDYDKLILEIWTKMNITPKQ
                     ALKEATSILIQHFRFINPSEDLAVSAGFVSQPPVQDTGAKTKGVEQSSDEEDIFGLSR
                     EILDTLIDNLDLTKRAKNCLKRERINTVAEILKKTPEELMKIKNFGKKSLDEIRKELK
                     DKFGIDYEKLFGERRSTKS"
     gene            complement(3524..4156)
                     /gene="rpsD"
                     /locus_tag="PLU28_06715"
     CDS             complement(3524..4156)
                     /gene="rpsD"
                     /locus_tag="PLU28_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOG34790.1"
                     /translation="MARYTGALCKLCRREGFKLYLKGERCYSEKCAMAKRNFAPGQHG
                     KDNKKLTQYGTQLRTKQALKRIYGILERQFRRYFEEAVKKIGQETGEVLMQILESRLD
                     NVVYEMGFAVNRRAARELVRHGHIRVNGKKVDIPSFRVRPGTVIEIKESSRGILQVKQ
                     GLELASKKGNTPSWLEVNAEQFKGTFTRMPKMDEISVPVDVQVIIELYSK"
     gene            complement(4182..4571)
                     /gene="rpsK"
                     /locus_tag="PLU28_06720"
     CDS             complement(4182..4571)
                     /gene="rpsK"
                     /locus_tag="PLU28_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004074888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOG34791.1"
                     /translation="MAANKKAVSSKKKKIAVEKGVVHIQSNFNNTIICLTDMTGNVIT
                     WASGGTSGFKGSKKGTPYASQLAADKVAKEALKIGVQKVDVVVKGPGSGRESAIRSVQ
                     AAGLNIENIQDATPIPHNGCRPKKKRF"
     gene            complement(4586..4969)
                     /gene="rpsM"
                     /locus_tag="PLU28_06725"
     CDS             complement(4586..4969)
                     /gene="rpsM"
                     /locus_tag="PLU28_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOG34792.1"
                     /translation="MARILGVEIPGNKKLFVALTYIYGVGYARAEEILTLTGIDTHKK
                     AKDLTDDEISKITRFINEHYKVEGDLRQENMKNVKRLIEIGSYRGYRHKAGLPVRGQK
                     THANARTRKGPRLSRISKKKTASAS"
     gene            complement(4987..5103)
                     /gene="rpmJ"
                     /locus_tag="PLU28_06730"
     CDS             complement(4987..5103)
                     /gene="rpmJ"
                     /locus_tag="PLU28_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HOG34793.1"
                     /translation="MKVRASVKKRCENCKIVRRAGRVWVVCDKNPKHNQRQG"
     gene            complement(5105..5362)
                     /gene="infA"
                     /locus_tag="PLU28_06735"
     CDS             complement(5105..5362)
                     /gene="infA"
                     /locus_tag="PLU28_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOG34794.1"
                     /translation="MAKKEDVIVIQGKIMEALPNATFRVVLENGHLVLAHISGRMRKN
                     FIRLLPGDKVTVEVSVYDLNRGRITRREKITKTEEIDEEDG"
     gene            complement(5375..6142)
                     /gene="map"
                     /locus_tag="PLU28_06740"
     CDS             complement(5375..6142)
                     /gene="map"
                     /locus_tag="PLU28_06740"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOG34795.1"
                     /translation="MIILKTENEITKMRRAGEKLARLLDDLLKSVIVPGASAHDVEQY
                     SVSYMKEIGAKPILIGYNGYPYATCVSVNDEVIHGYPLKSKVFKDGDLVSVDLDIEYD
                     GMITDAARTYAVGTITDKEKELIQVTEKSFFIGLEQAVIGKKTGDIGYAVQSYAESFG
                     FGVVRDFVGHGVGRKVHEDPSIPNYGYKGTGPMLRKNMTITIEPMISLGNYEVEILQD
                     GWTVVTIDKSKSAHYENTIVITDNGPEVLTAVTGYNP"
     gene            complement(6139..6786)
                     /locus_tag="PLU28_06745"
     CDS             complement(6139..6786)
                     /locus_tag="PLU28_06745"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HOG34796.1"
                     /translation="MRLIFFGPPGAGKGTQAKEVSERFSAVHISTGDILRQAVNSGSE
                     LGLKVKDILDKGLLVSDDIMNELIKLRLSKEESFLLDGYPRTIPQAEFLDGILSELSK
                     PVDCAVLIDADEEDIVKRISSRRVCPKCKRVYNTISLKPLKEGICDDCDSQLVHREDD
                     QEDVVRKRFKVYLSNTAPVIEYYRKNNKLVTVDGTKDVAEVTKELFNILEGVPHK"
     gene            complement(6791..>7203)
                     /locus_tag="PLU28_06750"
     CDS             complement(6791..>7203)
                     /locus_tag="PLU28_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOG34797.1"
                     /translation="DSFFKLGGAFYLIVYAILIIFFTYFYSSVVMDPKEVSDNIKNYG
                     GFIPGIRPGDPTSKFITTSMNRVTFIGAIFLVLISLLPYVIRSSSGVEIWIGGTSALI
                     AVGVALDMLQQIESHMIARQYEGFMKKGKLRGRR"
BASE COUNT         1873 a         1595 c         1242 g         2493 t
ORIGIN      
        1 ctttgaaggt tttttttcgg tcttaattga aaatgatgca gaaattacac caccaaggca
       61 gcttccaaaa agaacataat cagaaggtac cgtcctttca agaaactcaa atatactgta
      121 aaaatccagc tcctcttttg cagcataaag attcccaaat ccctgcattc ctatcatatg
      181 ggctttatag ccgctatcgc taagcctctg ggccattttt tcatagaccc ccggatactc
      241 aaacaacccg tgtaaaatga aaatatcttt tttaaagtcc gaacggttaa ggatatatct
      301 gtatgaaata ttttccattt aatatctccc caaaaatatc agatcttctg cttctctacc
      361 gcttcataaa gatcctggtt ttctgtatgt tttttcatta aattcatgaa aaatgtcaga
      421 gcttcctcaa catttacatc atttataaac tgtctgagta taaacatgtt tttcatattt
      481 ttttctgaga gcaacagttc ttcctttctc gttcctgaca gtttaaggtt tatagctggg
      541 aatatgcgtt tatctgaaag ttctcttgag agaattaact ccatatttcc tgttcctttg
      601 aattcttcaa atataagctc atccatcttt gaacctgtgt ctacaagggc agttgcgatt
      661 attgtaagac ttccgccttc tcttatcttg cgtgcagctc cgaaaaactt ttttggaaag
      721 cttaatgccg caggatcaac tccgccgctc aaaagctttc cgcttgaagg aactacaagg
      781 ttataggctc ttgcaagcct tgtaaggcta tccatgagga taaccacatc atgcccgaac
      841 tctacaagct ttttagcaag atgcagggtc atttccgcaa cttttatctg atccttagga
      901 tccatgtcaa agggagcggc aataacattt gcattgacag tatcctttag atcggtcact
      961 tcttcaggtc tttcatctat taaaagtacg aatcttttcg tattcggaaa atttttggct
     1021 atggagttgg cagtatcttt aagaagagtt gtttttccag ccttgggagg agcaacaata
     1081 agtgttctct gcccaaaacc tatcggagat atcatatcta ttattcttgc acttataggt
     1141 ccggatttat tctcaagtct catctgtcta tcagggtact cgggagtaag gttttcaaat
     1201 gagattctgt ccctggcttt ttcgggagcc tgatagttta ttgcttcaat tcttaagaga
     1261 gcaaagtatt tctcaccgtc tttgggcggt cttacctgtc ctgcaatcac atcacctgta
     1321 aaaagattga atctttttat ctgtgtgttt gaaacataaa tatcgtcact gccctggagc
     1381 agtgagttat caatgctgcg taggaatccg tatccatcag aaaatatttc cagtattcct
     1441 tcatggaaga agtaccctat cgattctgtc tgttttctta gtattgaaaa tataagttca
     1501 tgatctgaca tctttgagta gttggttatc tcaaaatttc tggcaagtgc gtaaagctgg
     1561 gttctggtca tatcgttcag cttggccatg tccagttcaa cggggcgtat ctggtctttt
     1621 tcggttcctt tgttctgatc ctgtttatga ttattggtat ctgtcaaaaa aatcacccct
     1681 gcttttaaaa gattttaata ttgtctttct ctgccgtttc ttattaaaga ttgttttagt
     1741 tattcacaag gtaagcttca aaaacatctt ggagtaatcc ggagcatttt cagttctgaa
     1801 aaagaggctg taaagctttt cataggaaga atatgcttga acgaagtttt tagaaagttc
     1861 ctgataaaaa aacggggttt ataacgcact acttttatac aataatatca caaaaaagtt
     1921 tttttgaaca gtaaaattat agtataaatg ggaggcaaaa gcctcccatg atgttcttat
     1981 ttttcttctg tcggaataag ctgcaacaat gaaagttcgg cagcatctcc tcttctgaaa
     2041 ccaattttaa gtactcttgt aaaaccgctt accttgttgc tgtacttagg agcgatctcg
     2101 tttactatct tggcagtcag ttttctgtcg ttgaaatttc tgttgatatc tcttgataga
     2161 gccatctttc ttgaagcatc agcagttgtg cttgcttcct tggcctttga gagaactctt
     2221 tccaccaacg actgagctac cttagcctta gcagatgtgg ttattatgct tccgctttcg
     2281 aaaacgtttc tggcaagatt tttaacaaga gcattcctat gtgaggcagg tctgcttaac
     2341 ttattcattt ttactctatg tctcatgact tggtgctcct cctttcaccg aataacttct
     2401 cataatcaat tccaaattta tctttcaatt cttttcttat ctcatccaaa gattttttcc
     2461 cgaaattctt gatcttcata agctcttcag gagtcttttt aagtatttcg gccacggtgt
     2521 ttatcctttc acgcttaaga cagttcttgg ctcttttggt aaggtcaaga ttatctatga
     2581 gcgtatccag aatctcccta gacagaccga aaatatcttc ttcatcggat gattgctcta
     2641 cacctttagt cttggcccct gtatcctgga caggaggctg actgacaaat cccgcagaaa
     2701 cagctaaatc ttcagaagga ttaataaatc taaagtgctg aatgaggata gaggtagctt
     2761 cttttaaagc ttgtttcgga gtaatgttca tcttcgtcca tatctcaagg ataagtttgt
     2821 cgtaatctgt tctctttccg actcttatgt tttctgtaag atagttgact ttcagaacag
     2881 gactgaaaac tccgtcaata tagataaact ctatatcgtt ttcgtgctcc atttcagaag
     2941 ctggtacaaa tccttttcct atctgtgcaa aaagctctat ctcaacttta tttttaccgt
     3001 ttattgtggc aatcttaaag tcaggattgg ctatagatat ctcagatgga acctcaatgt
     3061 cccctgctgt tatgtctgac ggaccctgtt tgttgatctt taggtgaagt tcaccttcaa
     3121 tactgttcag ttttttgaga tcatcgacct taagctgaac tttcttaagg ttgagggcaa
     3181 tctctaggat gtcctctttt acaccttcta ttgtatcaaa ttcgtgaagc ttgttaggta
     3241 tacgaatgct tgttatggct actgccggaa tggaagagag caaaaccctt ctgagcgcat
     3301 ttccgatagt tatagcatag cccttttcta gaggatacaa aacatacttt gcgtatctta
     3361 ttccttcttc agccaaacgt tcttcttcta attgaaatac ctctggtttt attagtaatt
     3421 ccataagaat accggattca actctgaacc ggattgcaca cctcctttga tgacttttaa
     3481 gagcgaggtg gatctcaagc tacgaataga ataaaaacta aacttacttg gaataaagtt
     3541 cgataataac ctggacatca actggtacag atatttcatc cattttgggc attctggtaa
     3601 aggtaccttt aaactgttca gcatttactt caagccatga aggagtgttt ccctttttcg
     3661 aagcaagttc caaaccttgt ttaacctgca gtatacctct gctgctttcc tttatttcga
     3721 taactgttcc cggtcttact ctgaacgaag gaatgtcaac ttttttaccg tttaccctta
     3781 tatgaccgtg tcttacaagt tctcttgcgg ctcttctgtt cactgcaaaa cccatttcgt
     3841 aaacaacgtt gtcaagcctt gactcgagta tctgcatcaa cacttcacct gtttcctgac
     3901 ctatcttttt gacagcttct tcaaagtatc tcctgaactg tctctctaat attccgtata
     3961 ttctcttgag agcctgtttg gttcttaact gcgtaccgta ctgggtaagc tttttgttat
     4021 cctttccgtg ctggccaggt gcaaagtttc tctttgccat agcacatttt tcagaataac
     4081 atctctcgcc cttcagatat aatttaaagc cttctcttct gcagagcttg catagagctc
     4141 ctgtgtatct tgccattctg ttattccctc cttcaagtca attagaatct tttctttttg
     4201 ggtctgcatc cgttatgtgg aataggagta gcatcctgaa tattttctat gtttaatccg
     4261 gctgcctgca ctgatcttat agctgactct cttcctgaac ctggaccttt tacaactaca
     4321 tcaactttct gaactccgat ttttaaggct tcttttgcta ctttgtcagc agcaagctgc
     4381 gaagcatacg gagtaccttt ctttgagccc ttgaatccgg aagttccgcc gctggcccaa
     4441 gttattacgt tgcctgtcat atctgtcaga catattattg tgttgttgaa gttagactgt
     4501 atatgaacga ctcccttctc aacagcaatt ttctttttct ttgaagatac tgctttctta
     4561 ttcgctgcca tttaacaaac cctccttatg atgccgaagc tgtctttttt ttgcttattc
     4621 tgcttaatct tggtcctttt cgagttctgg cgttggcatg agttttctga ccacgaaccg
     4681 gaagacctgc tttatgcctg tatcctctat atgatccaat ttcgattaat ctttttacgt
     4741 ttttcatgtt ttcctgtctg agatcacctt cgactttgta gtgctcgttt ataaatctgg
     4801 ttattttgga tatttcgtcg tcagtaaggt ctttggcttt tttatgagta tcaatgccag
     4861 taagcgtaag gatctcttct gctcttgcat atcctacacc gtaaatatag gtaagagcta
     4921 caaaaagctt tttgtttccc ggaatttcaa caccaaggat acgtgccatc tatctttccc
     4981 tccttattaa ccctgtctct ggttatgctt gggattctta tcgcatacca cccaaactct
     5041 tccagctctt cttactatct tgcagttctc gcatcttttt tttactgatg ctctgacttt
     5101 catattatcc atcctcctca tctatttctt cggtcttagt aatcttctct cttctggtta
     5161 tcctgcctct gttaagatca tatacggaaa cttcaaccgt gaccttatct ccgggaagaa
     5221 gtctgataaa gttctttctc atccttccgg atatgtgagc aagcacaaga tgtccgtttt
     5281 ctaacactac tctgaaagtt gcatttggaa gagcttccat aatctttccc tgaattacta
     5341 tgacatcttc tttttttgcc atttaatacc cccttcaagg gttataacca gttactgcag
     5401 taagaacttc cggtccgtta tctgttatga ctatggtatt ttcataatgg gccgacttac
     5461 ttttatcaat ggttacaact gtccaaccgt cttgtagaat ttcaacttca taattaccaa
     5521 gcgaaatcat cggttcaatt gtaatagtca tattttttct cagcatcggt ccggttcctt
     5581 tgtagccata gttaggaata gaaggatctt cgtgtacctt cctgcccacc ccgtgaccta
     5641 caaaatctct cacaacaccg aaaccaaacg attctgcata tgactgaacg gcgtaaccta
     5701 tatctcctgt ctttttcccg atcactgcct gttcaagccc gataaaaaaa gatttctcag
     5761 ttacttggat gagctctttt tccttatcgg ttattgtacc gacagcatac gtcctggcag
     5821 catcggttat cataccgtcg tactcgatat caagatcaac agaaaccaga tcgccgtctt
     5881 tgaaaacctt ggatttcaaa ggatacccgt gtattacctc atcattcaca gatacgcatg
     5941 tagcatatgg ataaccgttg taacctataa gaatcggttt tgcacctatc tctttcatat
     6001 aggaaacact gtactgttca acgtcatgcg cactggctcc gggtactata actgacttta
     6061 acaaatcatc aaggaggcgg gcaagctttt ctccggcccg tctcatcttg gttatttcgt
     6121 tttctgtctt aagaattatc atttatgcgg tactccttcc agaatattaa acaactcttt
     6181 tgtaacttct gcaacgtctt tagtaccgtc tactgtaact agtttgttat tttttctata
     6241 atattctatt acgggcgcag tgttactgag ataaacctta aatctttttc ttacgacatc
     6301 ctcctggtca tcttccctat gcacgagctg tgaatcacaa tcatcacata taccttcttt
     6361 taatggttta agcgatattg tgttgtaaac cctcttacat ttaggacaga ctctcctgga
     6421 ggaaattctt ttaactatat cttcttcatc ggcatcaatc aagaccgcac aatccacggg
     6481 tttagagagt tctgaaagaa taccgtccaa aaactccgcc tgtggaatgg ttcttggata
     6541 tccatccaga agaaaacttt cttctttaga caaacgaagc tttataagtt cattcattat
     6601 gtcatcagag accaaaagtc ctttgtcaag aatatcctta accttaagcc caagctcact
     6661 gcctgagttt accgcttgcc tcaaaatatc tccggtagat atgtgaactg cagaaaatct
     6721 ttctgaaact tctttagcct gggttccttt tccggctccc ggaggcccga aaaaaataag
     6781 tcgcataaac ttacctcctt ccgcgaagct ttcctttctt catgaagccc tcatactgcc
     6841 tggctatcat atgggattct atctgctgaa gcatgtcaag tgcaactcca acagctataa
     6901 gagcacttgt tccacctatc catatctcga ctcccgagga acttcttatt acgtaaggaa
     6961 gaagtgatat tagaacaaga aagatagctc cgataaaagt gactctgttc attgaggttg
     7021 ttataaattt ggatgtcgga tcgccaggtc ttattccggg tataaagcca ccgtaattct
     7081 ttatattatc cgaaacctct tttggatcca ttacaactga actgtagaag taggtgaaga
     7141 aaataatcag tattgcgtag acaataagat aaaatgctcc accaagttta aagaacgaat
     7201 cat
//
LOCUS       DAOSHR010000105         7198 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_9035_length_7198_cov_5.202296, whole genome shotgun sequence.
ACCESSION   DAOSHR010000105 DAOSHR010000000
VERSION     DAOSHR010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 7198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7198
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9035_length_7198_cov_5.202296"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="PLU28_06755"
     CDS             complement(<1..369)
                     /locus_tag="PLU28_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOG34798.1"
                     /translation="MKKILVFLVLVVSVFSFSAPLVVKGSNTVLPIAQMWAEEMKKLN
                     PSINITLEGAGSSTGISALFNNTADIANSSRWLKASEIEQMGKEKKLFMPIVVAYDGI
                     AVVVNKSIKLDNISISTLKDI"
     gene            551..1084
                     /locus_tag="PLU28_06760"
     CDS             551..1084
                     /locus_tag="PLU28_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34799.1"
                     /translation="MDFKTINPFLLAMIQFAVLGSFGEAVALIIKKEKIKIFRFLYSA
                     VVWAVLGILIKFIFTGFNGFVYELVKNGIVPDNLFIKSFLKSFFTNWLFGPWLIIIHR
                     VLDSVILGKIELSWQKLKKSMFTLVWFWLGAHTVTFMLSPQWQMTLAAVWSFVLGLLL
                     SIFSSKDKTSEKNDSNT"
     gene            1106..1600
                     /locus_tag="PLU28_06765"
     CDS             1106..1600
                     /locus_tag="PLU28_06765"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOG34800.1"
                     /translation="MESLKAVITMENEEKIVVELYEKYAPLTVKNFIDLCRKNFYSGL
                     TFHRIVKGFVIQGGCPNGDGTGGPGYEIKGEFSTNGINNTLKHTKGVISMARSSDPDS
                     AGSQFFIMLDDHSHLDGNYAAFGKVIHGMEVVDKIGKSKVDFRDKPLVPVVIKSISIE
                     NETL"
     gene            complement(1604..2170)
                     /locus_tag="PLU28_06770"
     CDS             complement(1604..2170)
                     /locus_tag="PLU28_06770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOG34801.1"
                     /translation="MELSKLLEKQVFCVLDTETTGLSPRSGDRIVEIAIYKIDLSNGY
                     RLSDKYVKLINPERYIPLSVSKIHGIYDYHVEKAPKFYEICESFLEFIGSNILVIQNA
                     PFDLSFLNNELRLCSHLSLENEIIDTISMSWKVFKGHTRHNLDTICQRLNINTEVQRH
                     RAEGDVIITAEAFIKMRDILITKTEHSD"
     gene            complement(2235..2663)
                     /locus_tag="PLU28_06775"
     CDS             complement(2235..2663)
                     /locus_tag="PLU28_06775"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOG34802.1"
                     /translation="MVKKFNVKVNGKIFEVEVEETASLNSSTVVSSTPATAPAQPVKS
                     VAQVNEAKPEVKAAPVQEKAPQAEYSGEGFKVAAPLPGVIVEVVVSEGQKVNKGDHLL
                     TIEAMKMENVIPAEASGTVRNILVKKGDTVEGDQLLLVIG"
     gene            complement(2688..3242)
                     /locus_tag="PLU28_06780"
     CDS             complement(2688..3242)
                     /locus_tag="PLU28_06780"
                     /inference="COORDINATES: protein motif:HMM:TIGR01195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family transporter subunit"
                     /protein_id="HOG34803.1"
                     /translation="MKSIKKIITVFLFCFLIISAFSIDSALPTDSTIPQSIDVVQAKS
                     SVDQNAGADVKTSEGSFSGEKVWYVTYMGVIIVFIVLIILMFVFQLMKYMKLQDKPKH
                     KMNLPKPESKPEPVQQGVHMSKAVINDAEEEVAAIMAAIAVYESGSQYTVTSIKPAIN
                     KKSSKVHFSSMWGVAPSHTTWRTK"
     gene            complement(3254..4810)
                     /locus_tag="PLU28_06785"
     CDS             complement(3254..4810)
                     /locus_tag="PLU28_06785"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HOG34804.1"
                     /translation="MSDPKFLEKLEDYNKRLQKLYECGGKDKIDKQHSNGKLTARERI
                     EYLVDEGSFEEVDIFVKHRSTNFDLDKKQFPYDGVVTGFGKVNGKKIAIFSQDFTVQG
                     GSLGEMHAKKIIKIQDLAMKYGMPIVGINDSGGARIQEGVDALYGYGGIFYRNTLASG
                     VIPQITVISGPCAGGAVYSPAITDFTIMVDKTSQMFITGPKVIKSVTNEDVDGESLGG
                     ASVHNSKSGVAHLKASDDKQAMEYVKKLLSYIPSNNLETVPDDDGDFSFEIDKKIYDL
                     VSPDPKKAYDIKELISIIFDEGTFFEIQPDYAKNIVVGFARISGKSVGIIANQPKQLA
                     GSLDINASDKASRFIRFCDAFNVPIITFVDTPGYLPGVGQEHDGIIRHGAKLLFAYSE
                     STVPKVTVIIRKAYGGAYIAMASQHLGSDFVFAWPTAEIAVMGPDGAANIIFAKDIEN
                     SKEPETTRKEKIEDYKKQFANPYEAAAKGYIEDVIDPATTRAKIAKALDISMNKAEPR
                     PAKKHGNIPL"
     gene            complement(4841..5245)
                     /gene="mce"
                     /locus_tag="PLU28_06790"
     CDS             complement(4841..5245)
                     /gene="mce"
                     /locus_tag="PLU28_06790"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HOG34805.1"
                     /translation="MTEKIDHIGIAVKSIDEALKLYNDLLGIAPSHEEIMPERGLRVV
                     FIEIGGSRIELLQPVSDNSEVSAFLEKKGEGMHHIAYKVDDVKAMITKAKELGLRTLS
                     DEPKNGAHNTSVVFMHPKSANGVLVELVEHKK"
     gene            complement(5238..6173)
                     /gene="meaB"
                     /locus_tag="PLU28_06795"
     CDS             complement(5238..6173)
                     /gene="meaB"
                     /locus_tag="PLU28_06795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HOG34806.1"
                     /translation="MSWEQQYNLISDDFFKGRIYALAKMITLVENHPDSAWKIIETLK
                     KNTSGDTPTIGFTGSPGAGKSTLVSAYVSMMAAKNQKIGVIAVDPSSPFTMGAFLGDR
                     IRMRDISESESVYIRSVASRGSVGGLCDAIYDIVDVMKAFGFDKIIIETVGAGQSEIE
                     IVYVADTVLLVMSPDSGDEIQMFKAGIMEIADGYVVNKIDLPQANAFSVKLRNTLALE
                     NDVKSKRKVFEVSASKNLGVEELEKWIDQFYSEMSSNGEIFKRHERRLKRRVRSNLMR
                     VMDNIISSQEVGKNSLREFKSMIVKKYIQQEACDD"
     gene            complement(6151..6552)
                     /locus_tag="PLU28_06800"
     CDS             complement(6151..6552)
                     /locus_tag="PLU28_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HOG34807.1"
                     /translation="MEKRIRVVVGKPGLDGHDRGSKVVARALRDAGMEVIYTGIRQTP
                     EEIVNTVLQEDADILGLSILSGAHMKLCQKIIKLMKEKEINVPIFLGGIIPADDIPEL
                     KKMGVNEVFGPGTSFEDIIKRVKEIVLGTAV"
     gene            complement(6568..>7198)
                     /locus_tag="PLU28_06805"
     CDS             complement(6568..>7198)
                     /locus_tag="PLU28_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOG34808.1"
                     /translation="QALSAVLGGTQSLHTNSFDEALGLPTQESATIALRTQQIIAYES
                     GVTDTVDPFAGSYLIEHLTKDIEGKAMEYIKKIDDMGGMVRAVETGYVQKEILNSAYE
                     AQLRIEKKEDVIVGMNEFTTEKGSKPTPILKVDTSVEEKQKAKIKELKEKRDNNLVSE
                     KLSSLKQAAASKENLMPFIYEAVKAYATLGEITNVLREVFGEFRESVIL"
BASE COUNT         2044 a         1522 c         1146 g         2486 t
ORIGIN      
        1 tatgtcttta agagtggaaa tggaaatatt gtccagtttt atgcttttgt ttactactac
       61 cgcaattccg tcgtatgcaa caacaatagg catgaaaagc ttcttttcct tccccatctg
      121 ctcaatttca cttgctttaa gccagcgcga cgagttggct atatcggctg tattgttgaa
      181 taaagcagat attccggttg atgaacctgc tccttccaaa gttatattta ttgaaggatt
      241 aagctttttc atctcctcag cccacatctg agcaatggga agaacggtat ttgatccttt
      301 aactacaagc ggtgcagaaa aagaaaacac acttacaacc aaaaccagaa agaccagaat
      361 ttttttcata ctttcccctc ctattttttc tttcactatt attttaattc actaatgttt
      421 ttaccgtttt gttttttatt aagttttatt aagaaagttc aatcctaaat tattccttat
      481 taagattttt tctgatataa tttatatgtg atctttacta aactcaaata atctaactct
      541 ggaggaaaaa atggacttta aaacaatcaa cccttttctg cttgctatga ttcagtttgc
      601 agttcttgga agttttggtg aagctgttgc cttaattata aaaaaagaga aaatcaaaat
      661 tttcagattt ctttattccg ctgttgtatg ggctgtactt ggaattctga taaagtttat
      721 ctttactggc ttcaacggtt ttgtatatga gcttgttaaa aacggtattg ttccggataa
      781 tctttttatt aaaagttttc tcaaatcttt ttttaccaac tggctttttg gaccatggct
      841 tataatcata cacagggttc ttgattctgt tattttagga aaaattgaac tttcttggca
      901 gaagcttaaa aaatctatgt ttactcttgt ctggttttgg ctgggtgctc atacggttac
      961 atttatgctt tcgcctcaat ggcaaatgac tctagctgca gtatggagtt ttgtacttgg
     1021 actgctgtta agtatttttt cctcaaaaga taaaacatct gagaaaaatg attccaacac
     1081 ttaataatac atatggaggt agaacatgga aagtctgaaa gcagttatta ctatggaaaa
     1141 tgaagagaaa atcgtagtgg aattatatga aaaatatgct cctttgacag taaaaaactt
     1201 tatagaccta tgcagaaaga atttttattc tggccttacc tttcaccgta tagtaaaagg
     1261 atttgtgata caaggcggat gtcctaacgg agatggcact ggtggaccag ggtatgagat
     1321 caagggagag ttcagcacta acggaatcaa taacacactt aaacatacta aaggtgttat
     1381 ttctatggcc agaagcagtg atcctgattc agcaggttcg cagtttttta taatgctgga
     1441 tgatcattca catttagacg gtaattatgc agcattcgga aaggttatcc atggaatgga
     1501 agttgtcgac aaaattggaa aatccaaagt cgatttcagg gataagcccc ttgtacctgt
     1561 agtaattaaa agtatttcta tagaaaatga aacactctga atttcaatca gagtgttctg
     1621 tttttgtaat cagtatatct ctcatcttta taaaagcttc agcagtgatt ataacatcgc
     1681 cttctgccct gtgtctttga acttctgtgt tgatattcag ccgttggcat atggtgtcaa
     1741 gattatgtct ggtatgcccc ttgaatacct tccaggacat acttatagta tcaataattt
     1801 cattttcaag tgaaagatga gagcatagcc tcagctcatt attcaaaaaa gaaaggtcaa
     1861 aaggagcatt ctgtataact agaatattgc ttcctataaa ctctaagaaa ctttcacata
     1921 tttcgtaaaa cttaggagcc ttttccacat gatagtcata tattccatgt atctttgaaa
     1981 cagataaagg aatatatctt tctggattta taagttttac atatttatca gaaagtctgt
     2041 agccgttgct taaatcaatc ttataaatag cgatctctac aatacggtcg ccacttctgg
     2101 gtgaaagacc tgttgtttcg gtatcaagaa cacaaaatac ttgcttttct agaagttttg
     2161 aaagttccat aatatcacca taatattaaa tcctgccatt gattctcagc tatggcagga
     2221 tttgatttat ttaattaacc gatcaccaaa agaagttgat ctccttcaac cgtatcgcct
     2281 ttcttaacta gtatatttct tactgttcct gaagcctctg ctggtatcac attttccatt
     2341 ttcattgctt ctatagtaag aaggtgatcc cctttattaa ctttctgtcc ctcagaaaca
     2401 acaacttcaa ctatgacacc tggaagaggt gcagcaactt taaatccttc tcctgaatac
     2461 tctgcctgag gagctttttc ctgtacaggt gcagctttta cttctggctt ggcttcattt
     2521 acctgagcaa cagattttac tggctgcgca ggagcagttg ccggtgttga agatactact
     2581 gtagatgagt ttaaagatgc tgtttcttca acttctactt caaatatctt tccgtttact
     2641 tttacattaa atttctttac cacttttatc cctccagtta tattattcta ctttgtccgc
     2701 catgttgtgt gggaaggagc aaccccccac attgatgaaa aatgaacctt ggaagatttc
     2761 ttattaatcg ccggctttat actagttacc gtatattgtg agccactttc ataaactgct
     2821 attgctgcca ttatagcagc tacttcctct tctgcatcgt ttataactgc tttactcata
     2881 tgaactccct gttggaccgg ttccggcttg ctttctggct taggaagatt catcttatgc
     2941 tttggcttat cctgaagttt catatacttc ataagctgga aaacaaacat cagaataatc
     3001 agaacaataa aaactattat aactcccatg tatgtaacgt accatacttt ctctccagaa
     3061 aaagaacctt cggaagtttt tacatcagct ccggcatttt gatcaactga agactttgcc
     3121 tgaacaacat caatactttg cgggatagtg ctatctgtag gcaaagctga atcaatacta
     3181 aaagctgaaa ttatcaagaa gcaaaaaaga aataccgtaa ttattttttt tattgatttc
     3241 atggacacac cccttataat ggaatatttc catgtttctt tgcaggccta ggttctgcct
     3301 tgttcatact tatatctagg gcctttgcta tttttgccct tgtggttgca ggatctatta
     3361 catcttcaat atagcccttt gctgctgctt catagggatt agcaaactgc ttcttataat
     3421 cttctatctt ctcttttctt gtagtttcag gttcttttga gttttctata tctttagcaa
     3481 aaattatatt tgctgcacca tcaggaccca taactgctat ttctgcagtc ggccatgcaa
     3541 aaacaaaatc agaccctaga tgctgcgaag ccatagcaat ataagcacca ccatatgcct
     3601 ttcttataat aacagtaact ttaggaacag ttgattctga gtatgcaaac aagagttttg
     3661 ccccatgtct tataattcca tcatgctcct gacctactcc tggtaagtaa cctggagtat
     3721 ccacaaaagt aattatagga acgttaaaag catcacaaaa tcttatgaat cgtgaagcct
     3781 tatccgaggc atttatatca agggaacctg caagctgttt gggttgattg gcgattattc
     3841 ctaccgactt tccagatatt cgtgcaaaac ctactactat atttttagca taatcaggct
     3901 gtatttcaaa aaacgttcct tcatcaaaaa tgattgaaat caattctttt atatcgtatg
     3961 cttttttagg atctggtgaa acaaggtcat atattttttt gtctatctca aatgagaagt
     4021 ctccatcatc atccgggact gtttcaagat tattggacgg aatataagaa agaagttttt
     4081 ttacatactc catagcctgt ttatcatcac tcgctttaag atgagctact ccactttttg
     4141 agttgtgaac acttgctcca ccaagactct ctccgtcgac atcttcattg gttacggact
     4201 ttataacctt aggacctgta ataaacattt gcgaagtttt atcaaccata attgtaaaat
     4261 ctgttatagc cggagaatag accgcaccgc cggcacaggg acctgagatg actgttatct
     4321 gtggaattac accagatgca agagtatttc tgtaaaatat ccctccgtat ccataaagtg
     4381 catccacacc ttcttgtatt cttgcccctc ctgaatcgtt tatccctact atgggcattc
     4441 cgtatttcat agccagatcc tgaattttta ttattttttt ggcatgcatc tcgcccaagg
     4501 atccgccctg aactgtaaaa tcctgagaaa aaatagcaat ctttttaccg tttacttttc
     4561 caaaacctgt cacaacacca tcatagggaa actgcttctt atcaagatca aaattggtgc
     4621 tcctgtgttt tacaaaaata tccacttcct cgaaagaacc ttcgtctact aaatactcta
     4681 ttctttctct ggcagtaagt tttccattcg aatgctgttt gtctatctta tcttttccgc
     4741 cgcattcata taacttctgc agccttttgt tataatcttc aagcttttct aaaaacttgg
     4801 gatctgacat cttttccgac ctcctatatt ataatggaaa ttacttttta tgttccacaa
     4861 gctcaacaag aactccgtta gccgattttg gatgcataaa aaccacactg gtattatgcg
     4921 caccgttttt aggctcatca gaaagagtcc taagtcccag ctctttggct ttggttatca
     4981 tagcttttac atcatctact ttgtaagcaa tatgatgcat tccttcgcct ttcttttcaa
     5041 gaaaagctga tacttcggag ttatcggaaa caggctgcaa taattctatc ctgctccctc
     5101 caatttctat aaatactact ctcagacctc tttctggcat aatctcttca tgtgaaggtg
     5161 ctattcccag caaatcatta taaagcttaa gcgcttcatc tattgattta actgctattc
     5221 ctatatgatc aatcttttca gtcatcacat gcctcctgct gaatatattt ttttactatc
     5281 atactcttaa actctcttag actgtttttc ccaacttcct gagatgaaat aatattatcc
     5341 attactctca tgagattgct tctaaccctt cttttaagcc tgcgctcatg acgtttaaaa
     5401 atttcaccat tagaactcat ctcgctgtaa aactggtcta tccacttctc caattcctca
     5461 actccgagat tctttgaggc actgacttca aaaacctttc ttttggattt tacatcgttt
     5521 tccaaagcaa gtgtatttct aagctttacc gaaaaagcat tagcctgtgg aaggtctatc
     5581 ttgttgacaa catatccgtc tgctatctcc attataccag ctttgaacat ctgtatttca
     5641 tctccggaat ccggagacat aaccaataaa actgtatctg ctacataaac tatctctatc
     5701 tctgattgtc cagcaccgac agtctctatt attattttat caaaaccaaa agccttcatt
     5761 acatcaacga tatcgtatat ggcatcgcaa agaccaccta cgctgcccct tgaggcaaca
     5821 cttctgatat aaacactttc agattcagaa atatcccgca tccttatcct gtctcccaaa
     5881 aatgctccca tcgtaaaagg actgcttgga tctacagcta ttactccaat tttttgattc
     5941 tttgccgcca tcatacttac ataagcggaa accaaagtac ttttccctgc accaggacta
     6001 cctgtaaagc cgatggtcgg agtatctcca gaagtatttt ttttgagagt ttcaatgatt
     6061 ttccatgcag aatcgggatg gttttccaca agagtaatca tctttgctag cgcgtagata
     6121 cggcctttaa aaaaatcatc tgaaattaaa ttatactgct gttcccaaga caatttcctt
     6181 cacccttttt ataatatctt caaatgaagt gcctggacca aaaacttcat ttactcccat
     6241 ttttttgagt tcaggtatat cgtctgctgg aattattcct ccgaggaaga ttggaacatt
     6301 tatttctttt tctttcatca gttttattat cttttgacaa agcttcatat gagcgcctga
     6361 aagtatcgaa agacccaaaa tatctgcatc ttcctgcaaa acagtgttca ctatttcttc
     6421 gggagtctgc cttatacctg tatatataac ctccatacct gcatctctca aagcacgggc
     6481 aacaactttc gaccccctgt catgaccatc cagaccaggt ttgccgacta caaccctaat
     6541 tcttttttcc atcagaactc acctccgtta tagtataacc gattctctga attcgccaaa
     6601 aacttccctg agaacgtttg ttatttctcc aagtgtggca taagctttta cagcttcgta
     6661 tataaaaggc attaaatttt ctttagatgc tgcagcctgc ttaagacttg aaagtttctc
     6721 acttaccaaa ttgttatctc ttttctcttt aagttctttt atcttggcct tctgtttttc
     6781 ctcaaccgat gtgtcaacct taagtattgg tgtaggttta cttccctttt cggttgtgaa
     6841 ttcattcata cctactatta catcttcttt tttctctatt ctcaattgag cttcataagc
     6901 agagttaaga atttcctttt gaacatatcc tgtttcaaca gctctgacca ttccgcccat
     6961 atcgtctatt ttcttaatat actccattgc ttttccttca atatcctttg taaggtgttc
     7021 tataagatag gatccagcaa acggatcaac ggtatctgtt actccggatt cataagctat
     7081 aatctgctga gtcctaagag caatggttgc cgattcctgt gtaggtagtc caagtgcttc
     7141 gtcaaaggaa tttgtgtgaa gtgactgtgt tccaccaaga actgctgata atgcctgt
//
LOCUS       DAOSHR010000106         7034 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_9315_length_7034_cov_6.149592, whole genome shotgun sequence.
ACCESSION   DAOSHR010000106 DAOSHR010000000
VERSION     DAOSHR010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 7034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7034
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9315_length_7034_cov_6.149592"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="PLU28_06810"
     CDS             <1..732
                     /locus_tag="PLU28_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HOG34809.1"
                     /translation="LTFAPEAGSQKMRDVINKGIDEEEIFEVVQTAKQRGWNKLKLYF
                     MIGLPGETYEDVKAIAELCTRVKNRSKIRDLTINVSIFVPKPHTPFQYARFEEMQSIS
                     EKIRILSEVRKRGMILKVHDSRMSTLEAVMARGDEKLGRIIMKAALGYNAIFQQWDEN
                     FDYDKWMHVFEEEQIDYTWYLRERSTEEELEWEFMSCGVSREFLISEWKKSQEPSLTP
                     DCRWSKCSFCGVCFDTDGKIKVIKK"
     gene            complement(774..4223)
                     /locus_tag="PLU28_06815"
     CDS             complement(774..4223)
                     /locus_tag="PLU28_06815"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOG34810.1"
                     /translation="MRKFFMVLMVIAQAVLFFGIDVLMPSFNSSGMERMPCFFENTIY
                     YAGPNYDIFFSRLESNQWTAPQKVPGDINTAENEISPLVLRNGGKLVMYFGRYISSER
                     DYDFFRAEYNESKQIWENITLVPELCTTLQDWDIWVDSTESTAYVTTKGSFGDQKSVG
                     GRDIWMSKKTNGKWSVPVNVSEVNSSSDEWSVAVAPDGKIWFDSSRSDSIGGYDLYCY
                     DPAKKSIEHPAEDINTMYNERSPWTDGKLIVFSSADRKGNAGGYDIYIATLPKEQQSQ
                     PEKTESVKQDGDVKVMTITNLRSKVGDSYVYDGQTVEVEGTACVSTGQWHDKANYFSI
                     TDGTSAVLIYANGFAEPKVKKGDLVRVRGKVSVAGYTSDVNSLFVLPQSPEDIKILSA
                     DNKLPEATILFTDTSKENFKTFESMPITIFGKLHQYDNDGISRGFYVDGSSDKNFDDQ
                     AGGIKIKFYSYADVDITNLSNGDFVTVNGILVQDKDKNFYIRLTDIYSVKKQPKKMID
                     FLTTVAKADLVAIEQKPVSNYIEYSFPKNFQAITGLKVLKGTNIAEDMQGSFNGEKYF
                     LAMHKLEGTRTSDMAPWLYMLDSSKNTVSRINMSGEVDYIDSNKETLIFSIRVTPENP
                     KSSRDIYLMNLATNKVSIVSDSMSDDIEPAINSDSTVAAFTRIVNGRYTLIIKDLKSG
                     KEKVVCENARKPSWKGASIVFQVYNGSNWDIAEYDSMTSKTSFVLNSKFNEFYPAVSK
                     NGDLLFIADYDSGNEVYVYSSSKKSIVKVIESAYLPDKPFWAEKDMVGATIKTGSIYN
                     LAAKTVPPSIPVSGIIKKAEGLINSPRFGCPSIILNNERILKVALTQNTLKQVSISGK
                     SGSYTVPVISDARAVIPSYVPDGLYDLNIISEKDGVFYESREPNCVFLTKEKTEVKIL
                     QITDTHMGLSKKSENKDGLKVLIEKANNENADFIVITGDITDSAQYYEQDYPYLVNTM
                     DELCDLPVFVIPGNHDSQSAGKVVGKEIWKKVIGPVKWSFTYANWYFIGMDTGDNPYG
                     LVNGDVSSEQISWLAQELKTAKDNNMKIALFAHHNMFDTRWNFFEKEEVRSTLARMLS
                     NSNVLLAAFGHRHSDTVDLYGNITAVTTQNCLEKEKAGFRIIILDSFGIKSLNMSDPI
                     TSF"
     gene            complement(4429..5127)
                     /locus_tag="PLU28_06820"
     CDS             complement(4429..5127)
                     /locus_tag="PLU28_06820"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HOG34811.1"
                     /translation="MTENIFFIFMLLYGLFFKIINLLIPVKIATIILITVGFIILYKN
                     RQHLKMISFEFYKDKRFLRVLFMSLFYGLIIIAISFVYQGILSINPSVKEFFHGENSF
                     IKSLTTFNIENLSLFDFEGRNTFFNILLSWLFFPMIVIYEEVLFRGVFLEFLNKKYKI
                     NTVLSIIIVSALFSFMHMSNSIYGLTHFFITGILFCIVYIKEKNLLYSMLTHLIINIF
                     VLLLHIIGPTNITF"
     gene            complement(5473..6858)
                     /locus_tag="PLU28_06825"
     CDS             complement(5473..6858)
                     /locus_tag="PLU28_06825"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HOG34812.1"
                     /translation="MRKVIAVLSVLLISIGMFAYVNEGYVSPVVNVVKYASPAVVNIE
                     AVGKSQQSSLYDPYFDDFFKRFFGGELPDTQQDVTAIGSGFIFDKAGYILTNYHVVEN
                     SSKITVSMADGKKFDAKLVGGDSELDLAVIKIETQTDLPVLELGDSDKIDIGEWAVAI
                     GNPLGLKHTVTLGVVSAVNRRIAKPDGSGYYVDLIQTDAAINPGNSGGPLVNIHAQVM
                     GINTAIINTSEGVNLGFAIPINIAKRFASSIIKDGKFEKAYLGVVVQTVNEELVKALG
                     LKVETGALISDVEKDSPAEKAGIKTGDVIVKLDERVIESNEDLVSYLRAYSANTKANI
                     VINRKGQELKLNVILSSKESKIVTAKKSYFGITVREITADDISEMGLKKDLGGVMVET
                     ISSSSQSILGLKTGDIITSIAVNGKESKIATLKDWESVASTVKKDSYVALLVYRKSYG
                     NFYTKFYYRGE"
BASE COUNT         2151 a         1358 c         1196 g         2329 t
ORIGIN      
        1 cttacttttg cacctgaagc aggttctcaa aagatgagag acgttataaa taagggaatt
       61 gacgaagaag aaatttttga agttgttcaa actgcaaaac agagaggctg gaataaatta
      121 aaactttatt tcatgatagg actccccgga gagacctatg aggatgtaaa agccatagcg
      181 gagctttgca caagagttaa aaacagatct aaaataagag atctgaccat aaatgtttca
      241 atatttgtgc ctaagccgca tactcccttt cagtatgccc gttttgaaga gatgcagtca
      301 atatcagaaa aaataaggat tctttctgag gtaagaaagc gcggtatgat tcttaaagta
      361 cacgactccc gaatgagtac ccttgaagcc gttatggcaa gaggtgatga gaaacttgga
      421 aggataatta tgaaagcagc gcttggatat aatgccatct ttcagcagtg ggatgaaaat
      481 tttgattatg ataaatggat gcatgttttt gaagaagaac agatcgatta tacatggtac
      541 ctaagagaaa gaagtacaga agaagagctc gagtgggagt ttatgagctg tggagtttca
      601 agggagtttt taatttcaga atggaagaaa tcacaggaac cttcacttac acccgactgc
      661 agatggtcaa agtgcagttt ttgcggagta tgctttgata ctgacggaaa aataaaggtt
      721 ataaaaaaat aaaacaagaa gagtgaaaac tcttcttgtt ttattttatg aaatcagaat
      781 gaagttatag gatctgacat gttcaagctt tttattccaa aggagtccaa aattattatg
      841 cggaatccgg ctttttcttt ttcaagacag ttctgagttg ttacagcagt tatatttcca
      901 taaaggtcaa ccgtatcgga gtgtctgtga ccgaatgcag ctaaaagaac gtttgaatta
      961 ctaagcattc tggccaatgt acttctgact tcttcttttt caaagaagtt ccatcttgtg
     1021 tcaaacatat tgtggtgagc aaaaagagca attttcatgt tattatcttt tgctgttttt
     1081 aactcctgtg cgagccagct tatctgttcg ctgcttacat cgccgtttac cagcccgtaa
     1141 ggattatctc cggtgtccat tccgatgaag taccagtttg catatgtgaa agaccatttt
     1201 accggtccga taactttctt ccatatttct tttccgacta cttttcctgc actctggctg
     1261 tcatgattgc cgggaattac aaacacgggc aaatcacaca gttcatccat agtattgaca
     1321 agatatgggt agtcctgctc gtagtactgt gctgagtctg ttatatctcc tgttattact
     1381 ataaagtctg cattctcatt gtttgccttt tctattagaa ctttcagtcc atctttattt
     1441 tctgatttct tggataaacc catatgagta tctgttatct gaagtatttt tacttcagtt
     1501 ttttcctttg taagaaaaac acagttaggt tctcttgact cataaaaaac tccgtccttc
     1561 tcacttatga tattaagatc ataaagacca tccggaacat aagaaggaat aacagctctt
     1621 gcgtctgaaa taaccggtac tgtgtaactc cctgattttc cgctgataga tacctgctta
     1681 agtgtgttct gggtaagagc tactttcaat attctttcgt tattaagtat tatagacgga
     1741 caaccgaacc tagggctgtt tattaagcct tcagcttttt ttattatgcc tgaaaccggg
     1801 atagatggcg gtaccgtttt tgcggctaag ttatagatac ttccggtttt tatagttgcc
     1861 ccgaccatat ctttttcggc ccaaaaaggt ttgtcaggta aatatgcaga ttcaataacc
     1921 tttacgatac tcttttttga tgaactgtat acataaactt cattgcccga atcataatcg
     1981 gctataaaaa gcagatctcc gttttttgag acggcaggat agaactcgtt gaactttgaa
     2041 ttaagaacaa atgatgtttt ggatgtcatg ctgtcatatt cagcgatatc ccagttggag
     2101 ccgttataaa cctgaaaaac tatgcttgcc cctttccagc tgggttttct tgcattttca
     2161 cagacaactt tttcctttcc ggacttaaga tcctttatga ttagagtgta ccttccgttt
     2221 actatccgtg taaaggcagc aactgtagag tcactgttta ttgcaggttc aatatcatct
     2281 gacattgaat ctgaaactat gcttacttta ttggttgcaa gattcattaa atatatatcc
     2341 ctggagcttt tgggattttc gggagtaacc cttattgaaa aaataagagt ttccttgttt
     2401 gaatcgatat agtcaacttc tccactcata ttgattcttg atactgtgtt ctttgaactg
     2461 tcgagcatat aaagccatgg tgccatatct gaagttctgg ttccttctag tttgtgcatg
     2521 gcaaggaagt atttttcacc gttaaaagag ccctgcatat cttcggcaat attagtgccc
     2581 tttaaaactt tcaggccagt aatggcctga aagttttttg ggaaagagta ttcaatatag
     2641 tttgaaacgg gtttttgttc tatggcaacc aaatcagcct tagcaaccgt ggtaagaaaa
     2701 tcgatcatct tctttggctg ttttttgaca ctgtaaatat ccgttagcct tatataaaag
     2761 tttttatcct tatcctgtac aagaataccg tttacagtca caaaatctcc gtttgaaaga
     2821 ttagttatat caacatctgc gtaagaataa aactttatct ttattccgcc tgcttgatca
     2881 tcaaagtttt tgtcactgct tccatcgaca taaaaacctc ttgatatacc gtcattatcg
     2941 tactgatgaa gttttccaaa tatagttata ggcatagatt caaaagtctt aaaattttct
     3001 tttgatgtat cggtaaaaag aatagttgct tcaggaagct tgttatccgc cgaaagaatc
     3061 ttaatatcct caggagactg cggaagtaca aaaagactgt ttacatcaga cgtataacct
     3121 gcaactgaaa ctttacccct tacacgaacc aagtctccct ttttaacttt gggttctgca
     3181 aaaccattag catagattaa aacagcactt gttccatctg taatactgaa ataattagct
     3241 ttatcgtgcc actgaccggt tgaaacacaa gctgttcctt ccacttcaac agtctgcccg
     3301 tcgtacacat aactgtctcc aaccttactt ctcagattag ttatggtcat tacctttacg
     3361 tcgccgtcct gctttacgga ctctgttttc tcaggctgac tctgttgctc tttaggaagt
     3421 gtggcaatgt aaatatcata tccgcctgca tttccttttc tgtcagcaga actgaaaaca
     3481 attaactttc cgtcggtcca agggcttctc tcgttgtaca ttgtgtttat atcttctgca
     3541 ggatgttcaa tgctcttttt agcaggatca tagcagtaca gatcatatcc accgattgaa
     3601 tcgcttctgc tggaatcaaa ccatatcttt ccgtcaggcg ctacagctac agaccattca
     3661 tctgaagaag agttaacctc ggaaacattt accggaacgc tccatttgcc gttggttttt
     3721 ttgctcatcc atatatctct tccaccaaca gacttctgat ctccaaagct tccttttgtg
     3781 gttacataag cggtactttc tgtgctgtcg acccatatat cccagtcctg aagcgtagta
     3841 caaagttccg ggacaagagt tatattttcc catatctgct tactctcgtt atactcagct
     3901 ctgaaaaaat cataatccct ttctgaggaa atatatcttc caaagtacat aacaagcttt
     3961 ccgccatttc tcaaaacaag agggcttatt tcattttcag cggtatttat atctccgggt
     4021 accttctgcg gtgctgtcca ctgatttgat tcaagcctgg aaaaaaatat gtcgtagttg
     4081 ggaccggcat agtagatcgt attctcaaaa aaacacggca tcctttccat tccgcttgag
     4141 ttaaaagacg gcatcagtac atcaataccg aaaaacagta ctgcctgggc tataaccata
     4201 agtaccataa aaaattttct catattcaac ctccccaaaa aaattatgta caataaagat
     4261 agtaataaat attacatata aaaacaaata gattttggtt aatggagaaa ggtatagaca
     4321 aagaatcggg tataaaaact cttttttaag gtgaaaggtc agtaatcatt ttgtttcatc
     4381 tgcaaacaaa atagcggcat gcttaagcat gccgctaaaa atagatgttt aaaacgttat
     4441 atttgttggt ccaataatat gtaaaagcag tacaaagata tttattatca gatgagtaag
     4501 catagaatac aaaagattct tttcctttat gtagactata cagaaaagga ttcccgtgat
     4561 aaaaaagtga gtaagtccgt agatactgtt actcatatgc atgaaagaaa acagagccga
     4621 aacgattatt attgaaagta ctgtgttgat tttatatttt ttattcaaaa actctaaaaa
     4681 tacccctctg aaaagaacct cttcatagat cactatcatt ggaaagaaca accaagaaag
     4741 caggatatta aaaaaagtat ttcttccttc aaaatcaaat aagcttaagt tttctatatt
     4801 gaatgttgtt aatgatttga taaaagaatt ttcaccatgg aaaaactctt ttactgaagg
     4861 gtttattgaa agaataccct gataaacaaa agaaattgca attattatta gtccataaaa
     4921 aagagacata aataaaaccc ttaaaaaccg cttatcctta taaaattcaa aacttatcat
     4981 cttaagatgc tgtctgtttt tatataggat aatgaatcca acagttatta aaataatagt
     5041 tgctattttg acaggtatca gaaggttaat tattttgaaa aagagcccat ataacaacat
     5101 gaatatgaaa aagatatttt ctgtcataac ttctccttta tactggcatt tttttgaata
     5161 atttccattg ctttgagctt agaagataac taagagattt tgaaccaaga aatctaccgt
     5221 atcctcctgt atagtaaaat tttttaaata tgttattcta aaaaatactt ttaagtataa
     5281 taaaacttgt ttagctaaac aatataaatt ttaaacgttt ttcgttaaaa aaaattaaaa
     5341 aaaaattaca tttaaataaa atacacaagt aaacaaaaat ataaaaaaac ttacttaaaa
     5401 atacaacaag atcttttttg gaataaaata atagcggcat gcttatgcat gccgctaaaa
     5461 atagatgttt atttattctc ctctgtaata aaacttggtg tagaagtttc cataactctt
     5521 tctgtaaacc aaaagtgcga cataactatc ttttttcact gtggaagcaa ctgactccca
     5581 atctttaaga gtagctatct ttgattcctt accgtttact gcgattgagg ttattatatc
     5641 tcctgtctta agtccgagga tgctctgtga acttgaactt atagtttcta ccataactcc
     5701 tccaagatct ttcttaagac ccatctccga gatatcatct gctgttatct cccttaccgt
     5761 tattccgaag tagctctttt tagctgttac tatctttgat tccttgcttg aaagaatgac
     5821 attcagtttg agttcctgtc cttttctgtt tataactatg ttagctttgg tattggccga
     5881 gtatgcccta aggtaactta caaggtcttc gttactttct ataactcttt cgtcaagctt
     5941 tactattaca tcacctgttt ttatgcctgc cttttctgca ggtgaatcct tttctacatc
     6001 gcttatcaaa gctcctgtct caaccttaag tcccagtgct tttaccagtt cttcattaac
     6061 agtctgtact acaacaccaa ggtatgcttt ttcaaacttt ccatctttta ttatggatga
     6121 agcaaacctt ttggcaatat ttataggaat agcgaatcct aagttaactc cttcagatgt
     6181 atttattata gcggtattta ttcccatgac ctgtgcgtgg atgtttacaa gaggtcctcc
     6241 gctgttacct ggatttattg cagcgtctgt ctgtatgagg tctacatagt aaccggaacc
     6301 gtcaggtttg gctattcttc tgttaacagc actcacaact cccagagtaa ctgtgtgttt
     6361 tagtcccaga ggattaccta tggcaactgc ccactcgcct atgtctattt tgtcagagtc
     6421 accaagttca agaacgggaa ggtctgtttg tgtttctatt ttaattactg ccagatccag
     6481 ttctgaatct cctcccacaa gcttggcatc aaatttcttt ccatcagcca tacttacggt
     6541 tattttgctt gaattttcaa ccacatggta attggtgaga atgtaaccgg ctttgtcaaa
     6601 tataaaacct gagcctattg cagttacatc ctgctgggta tcggggagct ctccgccgaa
     6661 aaaccttttg aagaaatcat caaaatatgg atcataaagt gatgactgct ggcttttacc
     6721 tactgcttct atatttacga cggctggcga tgcatacttt acaacattta ccaccggact
     6781 gacataacct tcatttacgt aagcgaacat acctatgctg atgagcaaaa cagaaagtac
     6841 agctatgact tttctcataa aaacttcacc tccgtaatag ttcttatagt aagttttgta
     6901 ttattattat acattggcat cacttaaaaa aaacttaaaa actactctat gtattctaaa
     6961 atatcataaa agctgttaac tataatatca gcatccttaa cttctttccc tccgtatccg
     7021 tagttgcagc ctat
//
LOCUS       DAOSHR010000107         6943 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_9476_length_6943_cov_5.106562, whole genome shotgun sequence.
ACCESSION   DAOSHR010000107 DAOSHR010000000
VERSION     DAOSHR010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 6943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6943
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9476_length_6943_cov_5.106562"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1146)
                     /locus_tag="PLU28_06830"
     CDS             complement(<1..1146)
                     /locus_tag="PLU28_06830"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOG34813.1"
                     /translation="MKDFYSRLTLFVINKAKYILVAFLIATVFFGYMTTKLGVDSDIM
                     KVMPQDDPIILDYYREQNAMGNTDVMIVAFFLEPQTDPQVVAKNFYDRMSTYKNLKEF
                     TKTDMTFFLSSGFLNVTNTEVIEQITQNVNNVFDAVKSANPYDFKSFEYLYDTLDGIY
                     NLQQGLSQSDDNFIKSYYMLSVEKDIMIMGVSFIKPSTDLGFVNEVIPVVKQNLSEIE
                     KIHGIKTGLTGNYIYSYEANKALSKDFAITTTLTMVFTISLFYIAFGNIITTAIVFIC
                     LILATVISMGITQMMFGQLNIITTFVASITVGLGIDYGQHLLTRFINDYKKNNNMVDA
                     LYYTYKNSLTPIFYGVITAIVVFTTLLLMRLPAFTQMSVMTIAGLFVF"
     gene            complement(1143..1529)
                     /locus_tag="PLU28_06835"
     CDS             complement(1143..1529)
                     /locus_tag="PLU28_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34814.1"
                     /translation="MLVDFFNAIFRPSKIFQHKKESLLIPLIIIIIFLFTPVWLFNWK
                     ACAFLLMTAYFGASARNIFSDKNLFIPYIITFSPLVFGALGKNFLIASFIWMQGLRSY
                     IDIKNKNCFSLIIGISFELFLLWWFI"
     gene            complement(1536..2225)
                     /locus_tag="PLU28_06840"
     CDS             complement(1536..2225)
                     /locus_tag="PLU28_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34815.1"
                     /translation="MRIYGWLAVMLILTTVLSFFLYVFYGKTDREYIVIEKTPVLTIK
                     QSFNTKSIIDSFSSVKNISAFSFGMIDYKIFIGKYRYDLKEQILYLEPLNKDLYDELK
                     NLAVSIEKESFEEVKKIKYFDTVINGYTLSSKNFYIRYIPRVFLIDFSKIPPSNMTYI
                     RTRYFIFDKSMYKKGGFQKSFVEGLKNSGGVVLDSRLLGTDEIDVTLSLLKNNGITVS
                     SDLKLISFKGE"
     gene            complement(2228..3499)
                     /locus_tag="PLU28_06845"
     CDS             complement(2228..3499)
                     /locus_tag="PLU28_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOG34816.1"
                     /translation="MSRKIKSAISIVIIVSVFFSSWIFAAASQKKIQDIYEQQYQDPI
                     FMLLYYIDNIYYDKSNVDYEKILDSTLDGLISGLDDPFAWYFDKRQTNENRIEESKEY
                     GGVGLTVRYDTAKKAIVVVSPMSGTPGEKAGILPNDYIITVDGSPVSQIGYTPSVDRM
                     RGEPGTAVKLEVFRDGWEETKIIEVPRAKIETKSVQYDLIEYNNKKIAYIRLTNFAEK
                     SYEEMQTALSYFISKSKDGLVLDLRSNPGGFLNVAIDIGSMFVRNGKIVSVRYYNGEE
                     ETVIPEPEKYVNFLKDIPIVVLVNKGSASASEILTGALKDNSIATIIGKQTYGKATVQ
                     RPIQLPNGGEVWLPIGHYFTPNGTDIHLKGIDPDIDIDNPAVELKSVNPSESETTEAM
                     LSTTNKPYINLSADSQLLKAFEVITEKSGGK"
     gene            complement(3483..4298)
                     /locus_tag="PLU28_06850"
     CDS             complement(3483..4298)
                     /locus_tag="PLU28_06850"
                     /inference="COORDINATES: protein motif:HMM:NF020287.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HOG34817.1"
                     /translation="MRTINQDDFVLIYFEDDTTLLIKADGNKSSCHKGSIDTASFIGQ
                     EYGYTGESNKGARFIVARPTYDEYIFKLKRASQIAYPKDLGYVILNLGIKPGDTILEA
                     GCGSGGATGIFSKIVGSEGKVFSYEKRQDFIDLARTNLMKFSQYDNVIFKNRDIKDGI
                     DETNFDSVFLDLPNPQDYMDIVYGAMGNSSMLGVLVPTTNQVSEILLAMRNFEFKKID
                     VWETFIRRYKTNPYRLRPSDIMVGHTAFLIFARKTLGLGKTKLEGEEYYEQEN"
     gene            complement(4300..4557)
                     /locus_tag="PLU28_06855"
     CDS             complement(4300..4557)
                     /locus_tag="PLU28_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HOG34818.1"
                     /translation="MAKKQIMNRLKRIEGQIKGLQNMVEQERNCVEILTQLSAISGAL
                     DNTAKEIVKEYARNCFIKYESTQDEKLINELIENLSKFRRM"
     gene            complement(4677..5315)
                     /locus_tag="PLU28_06860"
     CDS             complement(4677..5315)
                     /locus_tag="PLU28_06860"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOG34819.1"
                     /translation="MKKIIVMLLMISLSISLFSQFKLDLKYSDTKDTAGTVLVFASAV
                     AAAYFFDEQIRLTVPKIENLSIDSLTDAKISLGISSALLISSFLFDSPEFKDVAFRSF
                     QAGAASAAVGYLLKHATGRARPFMEEGKNSFMGLAAASDNYLSFPSGHSIVAWAVVTP
                     YAQKYGQWLYILPATVSAMRIIEDKHWTSDVIAASALGFLLGAFMPYLEISF"
     gene            complement(5432..5507)
                     /locus_tag="PLU28_06865"
     tRNA            complement(5432..5507)
                     /locus_tag="PLU28_06865"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5472..5474),aa:Ala,seq:ggc)
     gene            complement(5566..6105)
                     /locus_tag="PLU28_06870"
     CDS             complement(5566..6105)
                     /locus_tag="PLU28_06870"
                     /inference="COORDINATES: protein motif:HMM:TIGR04518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HOG34820.1"
                     /translation="MKSRRIVMNALFVGLAIVLSRFLSVRIPIGGAEGVRFGVGTLPV
                     ILAGISLGPLDGAVVGAVSDLLGYFIYPMGAFMPQFTLTSLLYGLIPGLLFRYILRKK
                     LTFANILVTSMIAELIGISITPLLINQLFQIPWAVLMPPRIITFVLRVIINPMIIYEL
                     LKRVPQLRTGMILPTENKS"
     gene            complement(6159..6632)
                     /locus_tag="PLU28_06875"
     CDS             complement(6159..6632)
                     /locus_tag="PLU28_06875"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HOG34821.1"
                     /translation="MQKKSFNDKLASNGDLPKIIKIEQKKSEGKDSSVKTMLVAPASE
                     YDQVMRRIPKGKLITSDEIRTFLAKKYSADYTCQLTAGIFINIVANASEERKAQGSSD
                     LTPYWRTLKKYGQLNEKYPGGVQTQKNLLESEGHEVIQKGRKFFVKDYKKALYIL"
BASE COUNT         2011 a         1361 c         1303 g         2268 t
ORIGIN      
        1 gaatacgaaa agacccgcaa tggtcattac cgacatctgt gtgaatgccg gtagcctcat
       61 aagcagaaga gtcgtaaaaa caacaatagc tgttataact ccataaaaga ttggagtaag
      121 agagttttta tacgtgtagt aaagagcatc taccatattg ttgttcttct tgtaatcatt
      181 tataaaacgg gtaagaaggt gctggccgta gtcaattcca agtcctaccg ttattgaagc
      241 cacgaaggtg gttattatgt tgagctgacc gaacatcatc tgggttatgc ccatacttat
      301 aaccgttgcc agaataaggc agataaatac aatcgcagtg gttatgatat tgccgaacgc
      361 tatatagaaa agagatatag taaagaccat agtcaaggta gtggttatgg caaagtcctt
      421 tgaaagagct ttgtttgctt cataactgta tatatagttg cctgtaagac ctgtctttat
      481 gccatgaatc ttttctatct cagaaaggtt ttgtttgaca accggaataa cttcattgac
      541 aaatcctaaa tctgtgcttg gttttatgaa gctgacaccc attatcataa tatctttttc
      601 cacagagagc atataatagc tttttataaa gttgtcatca ctctgggata gaccctgttg
      661 tagattgtaa attccgtcca gtgtatcata aagatactcg aaagatttga aatcgtatgg
      721 attggcactt tttacggcat caaagacgtt attgacattc tgggtaatct gctctatcac
      781 ttcggtgtta gttacgttta aaaaaccgga tgaaaggaaa aaggtcatat cggttttggt
      841 aaactcttta agatttttat aggtactcat tctatcgtag aaatttttgg ctacaacctg
      901 tggatcagtc tgcggttcca aaaaaaaggc tactatcatt acgtccgtgt ttcccatggc
      961 gttttgttct ctgtagtagt ccaggattat ggggtcatct tggggcataa ccttcatgat
     1021 atccgaatct acaccaagct tggtggtcat gtatccaaaa aaaaccgttg caataaggaa
     1081 tgccactaat atatactttg ccttgtttat tacaaaaagg gtcagcctgg aataaaaatc
     1141 cttcatataa accaccaaag aaggaacagc tcaaaactta tcccgattat cagagaaaaa
     1201 cagtttttgt tttttatgtc tatatagctt cttaatccct gcatccaaat gaatgaagca
     1261 atgagaaaat tttttcccaa cgcaccaaaa acaagtgggg aaaaggtgat aatataaggg
     1321 ataaaaagat ttttgtcaga aaaaatattt ctggcacttg ctccgaagta ggcggtcata
     1381 agcaaaaaag cacaggcttt ccaattgaaa agccataccg gagtaaataa aaatataata
     1441 attattatca aaggaatcag aagcgattct ttcttatgct ggaaaatctt tgaaggtctg
     1501 aagatggcat tgaaaaaatc aacaagcatt ttggtttatt cccctttaaa acttatcaac
     1561 tttaggtcac tgctgacagt tataccgtta ttttttaaca gggagagagt tacgtcaatt
     1621 tcatctgtcc ctaatagtct tgaatcaaga actactcctc cggaattctt aagaccttca
     1681 acgaagcttt tctgaaatcc gccttttttg tacatgcttt tatcaaagat gaagtaacgg
     1741 gttcttatat aagtcatgtt tgacggagga atttttgaaa aatctataag aaaaaccctt
     1801 gggatgtacc gtatataaaa gttcttagaa ctcagagtgt atccgttgat gaccgtatca
     1861 aagtacttta tcttttttac ttcctcaaaa gactcctttt caatcgaaac ggcgagattt
     1921 ttgagctcat cgtaaagatc tttgtttaaa ggttcaagat aaagtatctg ttctttcaag
     1981 tcatatcggt actttcctat gaagatttta taatcaatca ttccaaaaga aaaggcggat
     2041 atattcttga cagaagaaaa agagtctatt atacttttgg tattgaaact ctgttttatg
     2101 gtaagaacag gtgttttttc tatgacaata tactctctgt ctgtcttacc gtaaaaaaca
     2161 tataagaaga aagagagaac ggttgtcagt atgagcatta ctgccagcca gccatagatt
     2221 ctcaaattta tttccctccg gacttttccg ttattacttc aaacgctttc aagagctgtg
     2281 aatcggcaga aaggtttata tacggtttgt ttgtggtact gagcatagct tcggtcgttt
     2341 cactttcgga agggttaact gatttaagtt caaccgcagg attatcaatg tctatgtccg
     2401 gatcgatacc cttaagatgt atatctgtgc cgttgggtgt aaagtaatga cctatgggca
     2461 gccatacctc tccgccgttt gggagctgta taggtctctg aacagtagct tttccataag
     2521 tctgctttcc tattattgta gcgatgctgt tatctttaag agctccagta agaatttccg
     2581 aagctgaagc ggatccctta tttacaagta caactatggg gatatccttc agaaaattga
     2641 catatttttc tggttcaggt atcacggttt cttcctcgcc gttgtaatat cttacggata
     2701 ctatctttcc gtttctcacg aacattgagc ctatatctat tgcgacatta agaaatcctc
     2761 ccggattgct tcgcagatcc agaaccagtc catccttaga ctttgagata aaatacgaaa
     2821 gagcagtttg catttcttcg tagctttttt cggcaaagtt ggtgagcctt atataggcga
     2881 tttttttatt gttatactca ataagatcat actgaactga tttggtctct attttggctc
     2941 tgggtacctc tattatcttt gtttcttccc aaccgtccct gaatacttca agttttacgg
     3001 ctgtacctgg ttctcccctc attctgtcta ccgaaggagt atagccaatc tgggaaacag
     3061 gtgatccgtc aactgttatt atgtagtcat tggggagtat cccggctttc tctcccggtg
     3121 tacctgacat gggactaacc acaactatgg ctttcttggc ggtgtcgtac cttactgtaa
     3181 gtcctactcc gccgtattcc ttgctttctt ctattctgtt ttcgttggtc tggcgtttgt
     3241 caaagtacca ggcaaagggg tcatcaagtc ctgaaattag accatcaagg gtggagtcga
     3301 gtattttttc atagtcaaca tttgacttat cgtagtaaat attatcgata tagtataaaa
     3361 gcatgaagat aggatcctgg tactgctgtt catagatatc ctggattttt ttctgggaag
     3421 cggctgcaaa gatccaagaa gaaaaaaaga cagatactat tattactatt gagatagctg
     3481 atttaatttt cctgctcata gtactcctct ccctccaatt tagttttccc taaacctagt
     3541 gttttccttg caaaaataag aaatgccgta tgccctacca ttatgtccga cggcctgagt
     3601 ctgtatggat tggttttata tcttctgata aatgtttccc agacatcaat cttcttaaac
     3661 tcaaagttcc tcatggccaa cagaatttct gaaacctggt ttgttgtagg aacaagcact
     3721 cccagcatgc tggaatttcc catagccccg taaactatat ccatataatc ttggggatta
     3781 ggaaggtcta gaaaaactga atcaaaattt gtttcatcta ttccgtcctt tatatccctg
     3841 tttttaaaaa taacattatc gtactgtgaa aatttcataa gattggttcg tgccagatct
     3901 ataaaatcct gtcttttttc gtaggagaaa acctttcctt ctgatcctac aatctttgaa
     3961 aaaatacctg tagctccacc gcttccgcat cctgcctcaa gaatcgtatc tccgggtttt
     4021 atcccaaggt tgagtataac atatcccagg tctttgggat atgcaatctg ggaagcacgc
     4081 ttaagtttga agatgtactc gtcataagtt ggtcttgcaa cgataaatcg ggctccttta
     4141 tttgattcgc ctgtataacc gtactcctgt ccgatgaagc ttgcggtatc aatactgccc
     4201 ttgtggcatg aagatttatt tccgtcagcc tttatgagaa gggtagtatc gtcctcaaag
     4261 tatataagga caaaatcgtc ctggtttatt gttctcaaat cacatcctcc taaattttga
     4321 aaggttctcg ataagttcgt ttatgagttt ctcatcctgt gtggattcgt attttataaa
     4381 acaatttctg gcatattctt ttactatttc tttggcagtg ttgtctagag cacctgatat
     4441 ggcactgagc tgggtaagta tctccacgca gttcctttcc tgttcaacca tgttctgtaa
     4501 accctttatc tgtccctcta tccttttcag ccggttcatt atctgttttt ttgccactgt
     4561 caaccctcct gccgttcata attcatatta attatactac aattaagatt tttacgatac
     4621 ttttttaaaa taaaaacgaa caggaaaaat caatttattc gtacttggcc aagatatcaa
     4681 aaggaaatct ccagatatgg catgaaagcg cctaaaagaa aacccaaagc cgatgccgct
     4741 attacatcgg atgtccagtg cttgtcttca atgattctca tggctgaaac ggttgccggc
     4801 agtatgtaaa gccactgtcc atatttttgt gcataaggag tgacaaccgc ccatgcaacg
     4861 attgaatgcc cggaaggaaa ggaaaggtag ttgtcagaag ctgctgctaa gcccataaaa
     4921 ctgtttttcc cttcttccat gaaggggcgg gctctgccgg ttgcatgttt gagcagataa
     4981 ccaactgctg cacttgcagc tccggcctga aaggaacgaa aagcaacatc cttaaattca
     5041 ggactgtcaa aaagaaaaga cgatatcaat aatgccgaac ttataccaag acttatcttt
     5101 gcatcggtta aagaatcaat cgaaaggttc tcaatttttg gtacagtcaa acgtatctgt
     5161 tcatcaaaaa agtatgcagc cgccacagct gaggcgaaaa ccaatactgt acctgcagta
     5221 tccttagtat cgctgtactt cagatcaagc ttaaattggg aaaacaaact aatgctcaga
     5281 gatatcatga ggagcatcac aataattttt ttcaaagtat cccgcctctt tttttaaaag
     5341 taattacgtg attagattta ggctttctct ctcagaaatt accagatccg gccatataaa
     5401 caaaaaaagc ccagactccc tgagcttttt atggtggagt cgatgggatt cgaacccacg
     5461 acttctacaa tgccattgta gcgtgctccc aattgcacca cgaccccctg aacttaaaca
     5521 caaatattat atattaatac ttcatctttg tcaagaccat ctctttcatg atttgttctc
     5581 agtagggagt atcatgcctg tccttagctg tggaacgcgt tttaaaagtt cataaatgat
     5641 catcggattt attatgacac gcaggacgaa tgtaataatc ctcggaggca taagcactgc
     5701 ccagggtatt tggaaaagtt ggtttataag cagtggggtt atagaaatac ctattaattc
     5761 agctatcatg gaagttacaa gtatgttggc aaatgtaagt tttttacgca gaatatatct
     5821 gaataaaaga cctgggataa gtccgtaaag cagcgaggta agagtgaact gaggcatgaa
     5881 tgctcccata gggtatataa agtagcctag aagatcagaa accgctccta ctactgcgcc
     5941 gtcaagcggt cctaaagaaa tgcctgcaag tataaccgga agtgtgccta ctccgaaacg
     6001 tactccttcg gctccgccta tcggaattct tactgacaga aatcgtgaaa gtactattgc
     6061 caatcctaca aacaaagcgt tcataactat tcttcttgat ttcatttttc ctccgcacct
     6121 ctatagatat gcttatatta tatcatatct ggtatattct ataatatgta aagagctttc
     6181 ttatagtctt ttacaaagaa ctttctgcct ttttgtataa cttcatgtcc ttcactttca
     6241 agcaaatttt tttgtgtctg gactccgccg gggtactttt cattaagctg tccgtacttt
     6301 tttaatgttc gccagtaagg cgtaaggtcg gagctgcctt gggcttttct ttcttcactg
     6361 gcattagcga ctatgtttat gaagatgcct gcagtaagct gacaagtata gtcagctgaa
     6421 tacttttttg ctaggaaggt tcttatctcg tccgaagtta ttaattttcc ttttggaatc
     6481 ctccgcatta cctggtcgta ctctgatgcc ggtgcaacca gcatcgtttt aacagaagag
     6541 tcttttcctt cagatttttt ttgttcaatt ttaataattt ttggcagatc accgtttgaa
     6601 gcaagcttat cgttgaaaga ttttttttgc atgaagtaac catgcctcct taatatttta
     6661 ttagctgtat ggtaaaatat taccacatat ttgtctgatg tgttttgcct aatagtttat
     6721 ttatttttag aaactttttt cttttatttt atcaataaaa ggttgcttta gggtataatc
     6781 tttctataga tgctgtattt cgcacagaaa tatatttttg gttccaggag gctgaaatgg
     6841 ataaaaaaat gacttacaat gagtatattc tttcccttaa tccctctatt gaggatgagg
     6901 taacaagtgc gcttgtaatg aaagattttc ttgaatttta tgt
//
LOCUS       DAOSHR010000108         6913 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_9539_length_6913_cov_4.675707, whole genome shotgun sequence.
ACCESSION   DAOSHR010000108 DAOSHR010000000
VERSION     DAOSHR010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 6913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6913
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9539_length_6913_cov_4.675707"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            192..689
                     /locus_tag="PLU28_06880"
     CDS             192..689
                     /locus_tag="PLU28_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HOG34822.1"
                     /translation="MNIIQTKIGTIDIEKHQIITFESGLPGFENLSKFIVYTDDSIAP
                     ILWLVSIEDSKIAFPVIPPKNVRIDYSFFIPQEVVDYLEISKEEDLVILSVLTIPQQS
                     QEISLNLIAPMLISLINKKGIQLILDNTQYKSKHLLKEELERSQNLLKQSAGEDNAGS
                     DEKNQ"
     gene            661..915
                     /locus_tag="PLU28_06885"
     CDS             661..915
                     /locus_tag="PLU28_06885"
                     /inference="COORDINATES: protein motif:HMM:NF014638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator"
                     /protein_id="HOG34823.1"
                     /translation="MLVLTRKTNEYVEIIAGDRHIKITILDSNRGTVKIGFDAEKDIK
                     IYRGEVYDEIVSQNISSVNTDIKSLKDISRIFPGGKKGEN"
     gene            905..1723
                     /locus_tag="PLU28_06890"
     CDS             905..1723
                     /locus_tag="PLU28_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34824.1"
                     /translation="MRIDPYIKEQLSPLFSEVKPPDVAILGGIEVEHTIKKCSDNYSI
                     ETFIGGNGTRTALELKGLGIVPSILAFLGQDEFLNMVLNFFDSRNISVRWTPNNLKTQ
                     RRLTFTDTGEVILQRNDYIITINPIFIEEFIYNVKAVLVNVERWNVDIIDFLSYEKKM
                     TFVHADLDIIDSFSFSCKTLFIKSNDIHKALEAASKANYESMIIFAHDEIYFDGLTIK
                     PDICSKEYRDQAVSVYEAVFIESVLHDMSYAQAHEYAFRYFAYVLANGSKIENI"
     gene            1970..2058
                     /locus_tag="PLU28_06895"
     tRNA            1970..2058
                     /locus_tag="PLU28_06895"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2004..2006,aa:Ser,seq:gga)
     gene            complement(2099..2641)
                     /locus_tag="PLU28_06900"
     CDS             complement(2099..2641)
                     /locus_tag="PLU28_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOG34825.1"
                     /translation="MKYSGLIAAGFGGQGVMLLGQLLSYAAMLDGKFTTWLPSYGPEM
                     RGGTANCTVVISDEYIASPVIDAPTELLAFNLPSMNKFVSQIIKGGSLIINSSVVESV
                     PERKDIKIYRIPANDIAEKLGNIKVQNMVMMGAFLHVSNIVSMDAVQHAFEHKLTGTK
                     AKLIDLNVKAVESGMVAAGK"
     gene            complement(2638..3393)
                     /locus_tag="PLU28_06905"
     CDS             complement(2638..3393)
                     /locus_tag="PLU28_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOG34826.1"
                     /translation="MTYTERYKGPKSLTQKEFTYCPGCHHGIIHRLVAEVIDELGVQG
                     KTLMVAPVGCSVFSYEFFDVDGTVAPHGRAPAVATGMKRARPDRIVFTYQGDGDLAAI
                     GTGEIIQAANRGEKITTIFVNNAIYGMTGGQMAPTTLVGMKTTTTPYGRNPANEGFPI
                     HVSEVLKEITGVAFLARTKVSTPQDVNKTKQLIKKAFMAQINNVGFGLVEVLSTCPTN
                     WGLTPVESLKWLEENMTKEFPLGVYVDKVGDIK"
     gene            complement(3410..4477)
                     /locus_tag="PLU28_06910"
     CDS             complement(3410..4477)
                     /locus_tag="PLU28_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HOG34827.1"
                     /translation="MSGKVMVKGTEAIAEAAIRAGCRLYFGYPITPQSELPEYMARRM
                     PEKDVNGVFLQAESEVAAVNMIYGAACTGKRSLTSTSSPGFSLMQESMSYMAGAELPA
                     VFVNVVRGGPGLGDIQPAQCDYFQATKGGGHGDYRLLVYGPESLQEAVELTVKAFDMA
                     EKYRNPVLLLADGLLGQMMEPVEFPDFTDLEKLEKNSSWALQGTELTRKAHKVTSFNI
                     DPVGLEKMNINLQKKYAEMEKNERMYEEYMMEDAEVVIVSYGTIGRIAKSVAKMARNK
                     GIKAGVFRPITLWPFPYAELAKWARKETKLFFTVEMSAGQMIEDVRLAVEGRCPVKFY
                     GRMGGMVPTPTEIFDKLMELV"
     gene            complement(4494..4712)
                     /locus_tag="PLU28_06915"
     CDS             complement(4494..4712)
                     /locus_tag="PLU28_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HOG34828.1"
                     /translation="MEYKNLTIIDQERCKGCGLCINVCPKKVLDFSKNYNSKGYHYSE
                     VVKPQECIGCGFCYRMCPDVCIVVKTSA"
     gene            complement(4757..5425)
                     /locus_tag="PLU28_06920"
     CDS             complement(4757..5425)
                     /locus_tag="PLU28_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CobQ"
                     /protein_id="HOG34829.1"
                     /translation="MGNRITDHKVHIFVGMFGSGKTEAAINFSIRMRNIFDKVSIADI
                     DIISPYFRTRDKAEFLENKDIKVILPPKIYLNADLPIISPAVGGYISNPDYKIILDVG
                     GNDDGIMVIGSLNNFLQNADAAVYFVINTMRPFTDSEEKISSHIDRLSQKARINVNYL
                     ICNTNIIEQTSQGDLTLGEKMVMRVSQSKNIPVAFTMVNEDRKDLQTLNERFEIKRFM
                     KTEW"
     gene            complement(5428..6495)
                     /gene="buk"
                     /locus_tag="PLU28_06925"
     CDS             complement(5428..6495)
                     /gene="buk"
                     /locus_tag="PLU28_06925"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HOG34830.1"
                     /translation="MFRILVINPGSTSTKLAIYEDENEVLNQTVRHSAAELSGFEKIT
                     DQYKFRKGVIESFLSMNGYPANSFSAIIGRGGLLRPIEGGVYEVNESMLLDLKAAKNG
                     EHASNLGALIAYELAKTVQIDAYIADPVVVDEMMDIARISGHPDISRRSIFHALNQKA
                     IARQLAEKIGKRYDDSHLIIVHMGGGISVGAHKDGRVIDVNNALDGDGPFTPERSGTV
                     PLTGLIDLCFSGKYTYDEVKKLIKGKGGLVSYLGTNDAQEVQRMMKSGNNNAAIIYSA
                     MAFQISKWIGKMSAALDFEVDAIGLTGGIAYDKEYIVPWIKERVSFIAPVYVFPGGDE
                     ERALALSALRVLRNEEKLRTY"
     gene            complement(6506..>6913)
                     /locus_tag="PLU28_06930"
     CDS             complement(6506..>6913)
                     /locus_tag="PLU28_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HOG34831.1"
                     /translation="AKKIGVSMPKVALVAALEQVNPKMQATLDAAVITQMNRRNQIKD
                     CIVDGPFAMDNAVSLHAAQHKGIISDVAGDADIIVMPEINAGNIFYKSMVFLAGAKVG
                     STILGAKKPVILTSRADSDEAKLNSIALSVLMA"
BASE COUNT         1969 a         1509 c         1224 g         2211 t
ORIGIN      
        1 gaaagctttt atttgtaatt tccgaaaatt caatatactt ttattcactt atggaaccca
       61 tgaatcctca gcttataaaa tcttggccgg ttacttcaaa cttatccacc ataaaagtat
      121 ggtagtttta tagtataata gcagggaggg gattttctcc tccctttcat actcataaag
      181 gaggctcggc aatgaatatt atacagacca aaatcggaac aatcgacatt gaaaagcatc
      241 agataataac ttttgaaagt ggtctccctg gttttgaaaa tcttagcaaa ttcatagtct
      301 atacagacga ttccatagcc cccatactct ggctggtaag tatagaagat tctaaaattg
      361 cctttccggt cattccgcct aaaaatgttc gtattgatta ctccttcttt attccgcagg
      421 aagtagttga ctatctggaa atatccaaag aggaagatct agttattctt tccgttctga
      481 ctattccgca gcagtcacag gagatctccc ttaatcttat agctccgatg ctcatatcgc
      541 tcataaataa gaaaggtatt cagcttatac tggacaatac tcagtacaag tccaaacatc
      601 tgctgaaaga agaactggaa agatctcaaa atcttcttaa acaaagtgca ggtgaggata
      661 atgctggttc tgacgagaaa aaccaatgag tatgttgaga taattgctgg tgatagacat
      721 ataaaaatca ccattctgga ctctaacaga ggtactgtga aaataggttt tgacgctgaa
      781 aaggatataa agatataccg tggtgaggtt tacgatgaaa tagtttcaca gaatatttca
      841 tcggtaaata cagatattaa atcccttaaa gacatatcaa ggatttttcc gggaggaaaa
      901 aagggtgaga attgatccat atattaagga acagctttct cctttgtttt ctgaggtcaa
      961 gccgcctgat gtggcaatac tgggcggcat agaagttgaa cacacaatta aaaagtgctc
     1021 agacaactac tcgatagaaa cgtttatagg aggtaacggg acacgtacgg ctctggaact
     1081 taaagggctg ggtatagtac cttctatttt agctttttta ggacaggatg agtttctgaa
     1141 catggttctt aatttttttg attccagaaa catttccgta cgatggactc ccaataattt
     1201 gaaaacccaa cgcaggctta catttaccga taccggagaa gttatacttc agcgcaatga
     1261 ctacatcata accatcaacc ccatatttat agaagagttt atatataatg tcaaagctgt
     1321 gcttgtaaac gttgagagat ggaacgttga tataatcgat tttctcagtt acgagaaaaa
     1381 gatgaccttt gtccatgcgg atcttgatat tattgattcc ttttcttttt catgcaaaac
     1441 gttatttatc aaatcaaacg atatccataa agcacttgag gctgcttcaa aggctaatta
     1501 tgaaagcatg attatcttcg cccatgatga gatatacttt gatggtctga ccataaaacc
     1561 ggatatatgc tcaaaagagt acagagatca ggctgtatct gtttatgaag cggtatttat
     1621 tgagtccgtt ctgcatgata tgtcttatgc acaagctcat gagtatgctt tcagatactt
     1681 cgcttatgtg cttgcaaacg gaagtaaaat agaaaacatt taatccgtaa acttgacaaa
     1741 tatgtttata cgtggtataa tttctctgtt aatgtaaaca gcttaattat ttctgttacg
     1801 cctgtaattt acaaagtaac ataataatga aaaaagctca aggggttctc ggggggacta
     1861 tatgtccaag ttgatgcttg tattgtacaa gcatcaattt tattttttat actatactat
     1921 tgactagttc ttccttctat gatataatat ataaggtttt gaaagatatg gagagatggc
     1981 cgagtggtcg aaggcgcacg cctggagagc gtgttacggc aaaaccgtac gtgggttcaa
     2041 atctcactct ctccgccata aaaaaatctg ctcataagag cagatttttt gtttttattt
     2101 actttcccgc agctaccatt ccactttcaa cagccttcac atttaaatca ataagcttgg
     2161 ctttcgtgcc agtgagctta tgctcaaaag catgctgtac agcatccata gaaacaatgt
     2221 tgcttacatg aagaaaagca cccatcataa ccatattctg cacttttatg ttcccaagtt
     2281 tttcagctat atcgtttgct ggaattctat aaatttttat atcctttctt tcaggcacac
     2341 tttcaacaac cgaggagtta attatgagac ttccgccttt aatgatctga gatacaaact
     2401 tgttcataga aggcaggtta aatgcaagaa gctcagtagg tgcatctata accggtgaag
     2461 caatatactc atcggatatt accaccgtac agttggctgt tccgcctctc atctcaggac
     2521 cataagaagg aagccatgtc gtgaacttac cgtcaagcat ggcggcgtaa gaaagaagct
     2581 gccccagaag cataactccc tgtcctccaa aacctgctgc aattaatcca ctgtacttca
     2641 tttaatatca cctaccttgt ctacatatac acccagagga aactccttag tcatattttc
     2701 ttcaagccat ttgagcgatt caacaggagt aagtccccag ttggtcggac aagttgaaag
     2761 aacttcaacc aaaccgaatc caacattatt tatctgagcc ataaaggctt ttttgataag
     2821 ctgtttggtt ttattgacat cctgaggagt gcttacctta gttcttgcaa gaaacgcaac
     2881 tccggttatt tccttcagaa cttctgaaac atgtatcgga aaaccttcat tggcaggatt
     2941 tcttccgtaa ggtgtggtgg tagtcttcat tccaaccaat gttgtaggag ccatctgacc
     3001 gcctgtcatt ccatatatag cgttattgac aaatatggtt gttatctttt cgcctctgtt
     3061 ggcagcttgt ataatctctc cggtacctat agccgcaaga tctccgtcac cttgatatgt
     3121 aaaaactatt ctgtcgggtc ttgccctttt cattcctgta gccacagcag gtgctcttcc
     3181 gtgaggggct acggtgccgt caacatcaaa aaactcatat gaaaaaactg aacatccaac
     3241 aggagctacc ataagtgttt ttccctgtac tccaagctca tctattactt cagcaacaag
     3301 tctgtgtatt atgccgtggt ggcaacccgg acagtatgta aactcttttt gggttaaaga
     3361 ttttggacct ttgtatcttt ctgtatatgt catatgaata atccctcctt taaaccagtt
     3421 ccatcaattt atcaaatatt tctgtgggag tagggaccat tccgcccatt cttccataaa
     3481 acttaacagg gcatcttccc tctactgcaa gccttacgtc ctcaatcatc tgaccggcac
     3541 tcatttctac tgtaaagaaa agcttggttt cctttcttgc ccattttgct aactctgcat
     3601 atggaaatgg ccataaggtt ataggtctga atactccggc ttttattcct ttatttctgg
     3661 ccatcttcgc aacagacttt gctattcttc ctatagtacc gtaggaaact ataacaacct
     3721 cggcatcttc catcatgtac tcttcgtaca tcctttcatt tttttccatc tcggcatact
     3781 ttttctgaag attaatgttc atcttttcaa gccctacagg atctatattg aaagaagtaa
     3841 ccttatgtgc ttttcgtgta agttcagttc cctgaagagc ccaagaagaa ttcttctcaa
     3901 gtttttcaag atcggtaaaa tcaggaaatt caacaggctc catcatctgt cctaaaagac
     3961 cgtcagccaa cagaagaact ggatttctgt acttttcagc catgtcaaaa gcttttacag
     4021 taagctctac ggcttcctgc aaggattccg gaccatatac caaaagtctg taatctccat
     4081 gtccgccgcc cttggtagcc tgaaagtaat cacactgtgc cggctgtatg tctccaagtc
     4141 ccggacctcc tcttacaacg ttgacaaaaa ctgccggaag ttcggctcct gccatataag
     4201 acatcgactc ctgcataagt gagaaacccg gtgatgatgt tgaagtaagt gatcttttac
     4261 cggtacaagc tgctccgtat atcatattta cagcagcgac ttcgctttca gcctgtaaaa
     4321 atactccatt cacatctttt tccggcattc ttctggccat atattccggc aactcactct
     4381 ggggagttat aggatatccg aaatataatc tacagccagc tcttatagct gcttctgcta
     4441 tcgcttctgt tcctttgacc ataacttttc cgctcatttt agtaccccct tgattacgcg
     4501 ctggttttta caactataca tacatcagga cacattctgt aacaaaagcc gcatcctata
     4561 cactcctgtg gttttacaac ttcagagtaa tgatagcctt tgctgttata gttttttgaa
     4621 aaatccaaaa cttttttcgg acatacattt atgcagagcc cgcagccctt acatctttcc
     4681 tggtcaatga ttgtcaggtt tttatactcc atgggccaat acctccttta taaaaagtat
     4741 ctgatttgtc cttaaattac cattctgtct tcatgaatct ttttatttcg aacctttcgt
     4801 tcagggtctg aagatccttt cggtcttcgt taaccatagt aaatgcaaca ggtatgtttt
     4861 tgctctgcga tactctcatt accatctttt caccaagggt aagatcgccc tgactggttt
     4921 gttctataat gtttgtgttg catataagat agttcacatt tattctggct ttttgggata
     4981 atctgtctat atgcgaggaa attttttctt cactgtccgt aaacggtctc atggtattaa
     5041 ttacgaaata aacagctgca tcagcatttt gcagaaaatt atttagtgag ccgattacca
     5101 ttattccgtc gtcattccct cccacgtcaa gaattatctt ataatcagga ttgcttatat
     5161 atcctcccac tgccggtgag atgataggaa gatccgcatt aagatagatc ttggggggaa
     5221 gaataacctt gatatctttg ttttcaagga actccgcttt gtctctggta cgaaaatagg
     5281 gacttatgat atctatatcg gctatagata ctttatcaaa aatatttctc attcttatgg
     5341 aaaaatttat agcagcctct gtttttcctg agccgaacat tccaacaaag atatgaacct
     5401 tatgatcagt tattctgttt cccataatca gtatgtcctt aatttttctt catttctcag
     5461 aactctgagt gcagaaagcg caagagctct ttcttcgtcg ccacctggga aaacatatac
     5521 aggagctata aagctgactc tttcttttat ccacggaaca atatactctt tatcgtatgc
     5581 aattcctcct gtaagaccta tggcatcaac ttcaaagtca agcgcagctg acatttttcc
     5641 tatccatttt gaaatctgaa atgccatcgc agagtatata atcgcagcat tattattacc
     5701 tgatttcatc attctctgga cttcctgagc atcattagtt ccaagataag aaacaagacc
     5761 tcccttaccc ttgatcagtt tttttacttc gtcatacgta tacttcccac tgaaacaaag
     5821 gtctataagt cctgttaatg ggactgttcc agatctttcc ggtgtaaacg gaccatctcc
     5881 gtcaagagcg ttgtttacat caattaccct gccatcttta tgagcaccta cagatattcc
     5941 tccgcccata tgaactatta taagatgaga gtcatcatac cttttcccta ttttctctgc
     6001 aagctgacgt gctatagcct tttgattcag agcatgaaat atggatcttc tggaaatatc
     6061 gggatgaccg gatattcttg ctatatccat catttcatca accacaaccg gatcagcaat
     6121 atatgcgtcg atctgaacag ttttggcaag ctcgtatgcg ataagggctc ctaaatttga
     6181 agcatgttcg ccgttttttg cagctttcaa atccaatagc atactttcat ttacttcata
     6241 cactccacct tctataggtc ttagcagtcc gccgcgccct attatggcag aaaaactatt
     6301 tgcaggataa ccattcatgg aaagaaagct ttcaattact ccctttctga acttatactg
     6361 atcagttatc ttttcaaacc cggaaagctc tgcggcacta tgccttacag tctgattaag
     6421 tacttcgttt tcatcttcat aaatggctag cttggtagat gttgatcctg gattaatcac
     6481 aagtattcta aacatatgtc cccctttaag ccataagcac cgacaaagct atcgagttga
     6541 gcttagcttc gtcagaatca gctctggaag tcaggataac cggtttcttt gctccgagaa
     6601 ttgtacttcc gacctttgct ccggctagaa agaccattga tttataaaaa atattgcctg
     6661 cgtttatttc tggcatgaca attatatcag catcacctgc aacatcgctt attatacctt
     6721 tatgctgtgc ggcatgtaaa gaaacggcgt tgtccattgc aaacggaccg tcaactatac
     6781 agtcttttat ctgatttctt ctgttcatct gtgttatcac cgctgcatcc agtgttgcct
     6841 gcatcttagg attaacttgt tcaagtgcag caacaagagc aaccttaggc atacttacgc
     6901 ctattttttt agc
//
LOCUS       DAOSHR010000109         6878 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_9606_length_6878_cov_4.876008, whole genome shotgun sequence.
ACCESSION   DAOSHR010000109 DAOSHR010000000
VERSION     DAOSHR010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 6878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6878
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9606_length_6878_cov_4.876008"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..899
                     /gene="cas8b"
                     /locus_tag="PLU28_06935"
     CDS             <1..899
                     /gene="cas8b"
                     /locus_tag="PLU28_06935"
                     /inference="COORDINATES: protein motif:HMM:TIGR02591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas8b/Csh1"
                     /protein_id="HOG34832.1"
                     /translation="VQGRLQDYNLFEDDTNDFYINFVFFKITNSATKVMKLIKDVKPS
                     RLAKIFEKKKEIIKIFIEYFPENLVKGFNLKYVYFFSVQKIAGKNSYSDFRLCLETYD
                     AVFTGKLLNRQRVISRIVQIISQQFYSNSEYWEKSTADSVMYYKFLKEMGNIKEEEGM
                     DVSLLELDEKLRTYIKTMNYTEEQVTMFMLGFMVGRVGNQQNKLNETKPILRKINFHA
                     MDKPKIMRLANEVFEKMYQLKIDKYYEKEYGLCKSLLDKNIKNTGLSSVETVFYILSG
                     YAYSTNNWAKKSDKSENKEEEE"
     gene            896..1792
                     /gene="cas7b"
                     /locus_tag="PLU28_06940"
     CDS             896..1792
                     /gene="cas7b"
                     /locus_tag="PLU28_06940"
                     /inference="COORDINATES: protein motif:HMM:TIGR01595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas7/Csh2"
                     /protein_id="HOG34833.1"
                     /translation="MMNNGEILYMYDAKLCNPNGDPDEENRPRFDAEREINLVSDLRI
                     KRYVRDYLHEEEGKEIYVRKIEDKPVTAGERVKNFVKNGELDVENIKKELIDIRLFGA
                     TIPLKSDNKSITGPVQFNWGYSLNKVELMESSITSNFSSTSGNAQGSIGKDYRVKYSF
                     IAFSGVVSGRNAKKTGLTDEDLKLLDKAMKNAIPLNVTRTKINQYPRLYIRLEYKDTS
                     TVLGDMRKHVKLIKDTEEESIRDISELKLDITQLVNLFARNKSKIEKIYYFACDELKL
                     LCNNQTVKLTEVLKDLNVSLVE"
     gene            1798..2523
                     /gene="cas5"
                     /locus_tag="PLU28_06945"
     CDS             1798..2523
                     /gene="cas5"
                     /locus_tag="PLU28_06945"
                     /inference="COORDINATES: protein motif:HMM:NF021240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas5"
                     /protein_id="HOG34834.1"
                     /translation="MKAVVFDLVGKFAHFRKFYTNSSSLSYLIPPRTTLQGVCAAILG
                     YERDTYYEKLSKERFSLTVTIKSTIRRIMQTVNYISIKSESDIYKYTEHTQIPFEILC
                     GDDEIRYRVYASHKDEEINLKLYSMIKDNQTELPLYMGCAPFSCVTEFVGFFDLYKKE
                     PNQSIELHTPLNIKEIEGKSLDFSQELSLVKERMPADFTKERSCYEVNSYIYEETGKP
                     VYVKLKNIYYQVDSNERENIIFM"
     gene            2538..4865
                     /gene="cas3"
                     /locus_tag="PLU28_06950"
     CDS             2538..4865
                     /gene="cas3"
                     /locus_tag="PLU28_06950"
                     /inference="COORDINATES: protein motif:HMM:TIGR01587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HOG34835.1"
                     /translation="MDFFSHPGKLLKTHLQEVNDFSRYRIDSILKESGKIDKNIIKAC
                     EVQAFSHDFGKYTTYFQNYLLHGIESKCSRHSFISALFGAYVAQKILPQGNMPLFIYS
                     SILHHHGNESEYSKYLPRERKYDKKKDLIGDMDILDCQKQDLKKNIQSISSEYGNYGE
                     YVKEFLEKTEYDDLFLYLKKLNYNLSKEDEKNYFQQQLLYSLLIYADKLSAAGLRPVQ
                     TEIIKFTELNDNLKSNILKNDTQINKIRTEIYSKVLSSIEGKTNEKIFSITAPTGTGK
                     TYTGFMSAVKLREELNGTRKIIYVLPFTSIIDQNYFVIEKLFQHDKNYNAQSSEYLIK
                     HHHLTDFNYNDSEEKYDSSKSELLIETWESGVVVTTFVQLLETLISNKNSMLKKFNKL
                     CGSIILLDEVQAIPVDLYGVCEYVLEKASQELDCRIIMMTATKPMIFGESIELLENNK
                     EYFKKFNRTVLNIETDKKITVPEFAEVFECRYSDKNSYLTVCNTIKQSLEVYELLKIK
                     YGDKVKYLSTNLLPIHRKTVIDTVRQKLKDKEKIILVSTQVVEAGIDLDFDEVYRDIG
                     PLDSIVQCAGRCNRSGLDQKNNVYVVRMKDENDRGYAEKVYGRHLIEVTMEVLKNKTK
                     IEEVEYYDTIEEYFSVLKEKRSQNISNNFIKAIKQVDFSFGENKESISKFTITNERPD
                     LIDVFVVYDEKAQALLDKYKKLIGIKDYFQRHNEFLKIKKDLNEYILTIPLKYVSILQ
                     NSDKVLNNIPYEAVSTYYEADTGFKQNKDVNAFIW"
     gene            4859..5365
                     /gene="cas4"
                     /locus_tag="PLU28_06955"
     CDS             4859..5365
                     /gene="cas4"
                     /locus_tag="PLU28_06955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HOG34836.1"
                     /translation="MVSDGFAVNGVLIWYYNICKRQVWLMAHNILPDESHDDIQVGRF
                     IHENTFKRNDKEVYFGNVRFDVVFQSEEKIVIGETKKSSWNNKGSKYQLLFYLKLLKE
                     NGIEASGVLLYPEQKKRESIELTSENILEVERMQKEIIALISEDKPLPAEKNLYCKNC
                     AYSEYCYA"
     gene            5368..6360
                     /gene="cas1b"
                     /locus_tag="PLU28_06960"
     CDS             5368..6360
                     /gene="cas1b"
                     /locus_tag="PLU28_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated endonuclease Cas1b"
                     /protein_id="HOG34837.1"
                     /translation="MKKDIYIFKSGELKRKDNTLFFESQEGKKFIPIEETDNIYVFGE
                     VDINKRFLDYAGEKEICLHFFNYYGYYSGSYYPRQHLNSGFVTLKQAEYYLDYEKRVV
                     IAKNIILAALRNIQIVLRYYNKNKEDFNSTIEIIKENMLTIKESKTIEEIMAQEANSR
                     IAYYKAFNNIIKNPDFYFEKRTKRPPKDKINALISFGNSMMYVTVLSEIYKTNLDPRI
                     GYLHSTNERRFTLNLDIAEVFKPIIVDREIFSLLNKNILTTGDFKEDLNGTILKEDSM
                     KKFVQAYNEKLMTTINHYKLNREVSYKRLIRMELYKLQKHITGEATYEGFTAEW"
     gene            6369..6632
                     /gene="cas2"
                     /locus_tag="PLU28_06965"
     CDS             6369..6632
                     /gene="cas2"
                     /locus_tag="PLU28_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HOG34838.1"
                     /translation="MFAILVYDFNEKRVGKALKKCRKYLNWVQNSVFEGEITRGNLTK
                     LKMELQSIMKKDEDSIIIYTMENTKYSEREIIGLEKNEISQII"
BASE COUNT         2658 a         1008 c         1284 g         1928 t
ORIGIN      
        1 aagtacaggg aaggcttcaa gattataatc tttttgaaga cgatacaaat gatttttaca
       61 tcaactttgt tttttttaaa ataaccaata gcgctactaa ggtaatgaaa ctcattaagg
      121 atgtaaaacc aagcagactt gcgaagatat ttgaaaagaa aaaagaaatc ataaaaattt
      181 ttattgaata ctttcccgaa aatttggtaa aagggtttaa tttaaagtat gtatactttt
      241 tctcagtgca aaagatagca ggcaagaaca gttactcgga tttcagacta tgtcttgaga
      301 cctatgatgc ggtctttaca ggaaagctat taaacaggca gagggttatt tcaaggatag
      361 tacagataat ttcacaacag ttttacagca actctgaata ctgggaaaaa tcaacggcag
      421 attctgttat gtactataaa ttccttaaag aaatgggaaa tataaaggag gaagagggta
      481 tggatgtttc tttgcttgag cttgacgaaa aacttagaac atatattaaa acaatgaact
      541 ataccgaaga acaagttacc atgtttatgc tggggtttat ggtaggaagg gttggaaatc
      601 aacagaataa gttaaatgag actaagccta ttttgaggaa gataaacttt catgccatgg
      661 acaagccaaa gatcatgaga cttgccaatg aagtatttga aaagatgtat cagcttaaga
      721 ttgataagta ctatgagaaa gagtatggcc tttgcaaaag tctcttggac aagaatatta
      781 aaaatactgg gctttcctct gtagaaactg tgttttatat cctttcagga tacgcatact
      841 ccacaaacaa ttgggcaaaa aaatctgata aatcagaaaa taaagaggag gaagaatgat
      901 gaacaacggc gagatccttt acatgtatga tgcaaaactc tgtaatccaa acggtgatcc
      961 tgatgaagaa aacagacctc gtttcgatgc ggaaagagag ataaaccttg tttcagatct
     1021 aagaataaaa agatatgtaa gagattatct gcatgaagaa gaaggcaaag agatctatgt
     1081 aagaaaaatt gaagataaac cggttacggc tggagaaaga gtaaagaatt ttgttaaaaa
     1141 tggtgaattg gatgtagaaa atatcaaaaa agagcttata gatatccggc tttttggcgc
     1201 aactattccc ttaaaaagtg ataataaaag cattaccggt ccggtacagt tcaattgggg
     1261 gtactctctc aataaagttg aactgatgga atcatcgata acttctaatt tttcatctac
     1321 tagcggtaat gcacaaggta gcataggtaa agattacagg gtaaagtact cttttatagc
     1381 gttttccggt gtagttagcg ggcgcaatgc aaaaaagaca ggtctcacag atgaggatct
     1441 gaagcttctt gataaagcaa tgaagaacgc tattcccctg aatgttaccc gtacaaagat
     1501 aaatcagtat ccaaggcttt acatcaggct tgagtacaag gatacttcaa cggtattagg
     1561 tgatatgcgt aaacatgtta agcttattaa ggatacagag gaagaaagca taagagacat
     1621 atcagaactg aagcttgata taactcaact tgtaaacttg tttgcccgga ataagtcaaa
     1681 aattgaaaag atctattatt ttgcctgcga tgagttaaag cttttgtgca ataaccaaac
     1741 tgtaaagctt acggaggtac ttaaagatct aaacgtatct ttagtggagt gaaggtcatg
     1801 aaggcggtag tatttgatct ggtgggaaag tttgcacatt tcagaaagtt ctataccaac
     1861 tcctcttcct taagctatct gatccctccc agaacaacac ttcagggagt ctgtgcggcg
     1921 atacttggct atgaaagaga cacctactat gaaaagcttt caaaagaaag gtttagcctc
     1981 acggttacaa taaaaagcac aataagaaga ataatgcaga ctgtcaacta tataagcata
     2041 aaaagtgaga gtgatattta taagtatacc gagcatacgc agataccgtt tgaaattctg
     2101 tgtggcgatg atgagatcag gtatagagtt tatgcatctc acaaggatga agaaataaac
     2161 ctaaagttgt acagtatgat taaagataat caaaccgaac ttcctcttta tatggggtgt
     2221 gcaccgtttt catgtgtgac cgaatttgtt ggcttttttg atctttataa aaaagaacca
     2281 aatcaaagta tagagctgca tacacctctg aacataaaag aaattgaagg aaaaagtctt
     2341 gatttttctc aagagctctc actcgtaaaa gaaagaatgc ctgcagactt taccaaagaa
     2401 agaagctgct atgaagtgaa ctcgtatatc tatgaagaaa ccggaaaacc tgtttatgtt
     2461 aagcttaaaa acatatatta tcaggtagac tctaatgaac gtgaaaacat tatttttatg
     2521 tagacggaga tggaaagatg gatttttttt ctcatccggg aaaacttttg aaaactcatc
     2581 ttcaggaagt caatgatttt tctcggtata ggattgatag tattttaaag gaaagcggaa
     2641 agattgataa aaatattatc aaagcgtgtg aagtacaggc tttttcacat gattttggaa
     2701 agtatacaac atactttcag aattatcttc tgcatggtat agagtctaaa tgcagcaggc
     2761 actcttttat ctctgcgctt ttcggggcat atgtcgctca aaagatatta cctcagggga
     2821 atatgcccct ttttatatat agttcaatac ttcatcatca cggcaatgaa agcgagtatt
     2881 caaagtatct gccaagagaa cgaaaatatg acaagaaaaa agatttaata ggagatatgg
     2941 atattcttga ctgtcaaaag caggatctaa aaaaaaatat acaaagtatt tcatcagagt
     3001 atggtaacta cggggagtat gtaaaagagt tccttgaaaa aacagagtat gatgatctgt
     3061 tcctttactt gaaaaaactt aattataatc tttcaaaaga agatgaaaaa aattattttc
     3121 agcaacaatt gctatactct cttttaatct atgcagacaa actttctgcg gcaggtttaa
     3181 gacctgttca aactgaaata atcaagttta ctgaacttaa cgataatctg aagagtaata
     3241 tattgaagaa tgacacacag ataaacaaaa tcaggactga gatttattcg aaggtactaa
     3301 gctctataga aggaaaaaca aacgaaaaaa tcttttcgat aactgcccct acaggaacag
     3361 gcaagacata caccggattt atgagcgctg taaaacttcg tgaagaatta aacggaacaa
     3421 ggaaaataat atatgtttta ccttttacct ctataatcga tcagaactac tttgttatag
     3481 aaaaactatt ccaacatgat aagaactaca acgcccaaag ttctgaatac cttattaaac
     3541 accatcatct tacagatttc aattacaacg attcagaaga aaagtatgat tcctcaaaat
     3601 cagaactgct aatagaaaca tgggaaagcg gagttgtagt aaccacattt gtacagctac
     3661 ttgaaacact catttcaaat aagaacagta tgttaaaaaa atttaataag ctatgtggtt
     3721 ccataattct tttagatgaa gtacaggcaa tacctgtcga tctttacgga gtttgtgagt
     3781 atgtacttga aaaagcttca caggagcttg actgccggat tataatgatg acagcgacta
     3841 agcctatgat ctttggagaa agtatcgaac tgcttgaaaa taacaaagag tattttaaaa
     3901 aattcaacag aaccgttctc aatattgaaa cagataaaaa gattacagta cctgaatttg
     3961 cagaggtatt tgaatgccgc tattccgata aaaactctta tcttactgta tgcaacacca
     4021 taaaacagtc tttggaagtt tatgaactgc tgaaaataaa gtatggagat aaagttaagt
     4081 acctttcaac aaatctttta cctatacata gaaaaactgt tatagatacc gtgcgtcaaa
     4141 aacttaaaga taaagaaaaa ataattcttg tttctactca ggtggtggaa gcaggtatag
     4201 atcttgactt tgatgaagtt tacagagata taggccctct cgattcaata gtacagtgtg
     4261 cgggacgctg caacagaagc ggattagatc aaaaaaacaa tgtgtatgtt gtacgtatga
     4321 aagatgaaaa tgacagaggc tatgcagaaa aagtctatgg acgccacctt atagaggtta
     4381 ctatggaggt tttaaaaaac aaaacaaaaa tagaagaagt agagtactac gatactattg
     4441 aagagtactt ttcggtactt aaagaaaaga gatcacagaa catatcaaat aattttatca
     4501 aagcaataaa acaggtcgat tttagttttg gagaaaacaa agagagtata tcaaaattta
     4561 cgattacaaa tgaaagacca gatcttatcg atgtttttgt agtatatgac gaaaaagcac
     4621 aagctctttt agacaaatat aaaaagttaa taggtataaa agattatttt caaagacata
     4681 acgaatttct caagataaaa aaagatttaa atgagtatat tctgaccatt cctttgaagt
     4741 atgtatctat acttcagaac tctgataaag ttctgaacaa tattccttac gaagcagtaa
     4801 gcacttatta tgaagctgat accggattta aacagaacaa ggatgtgaac gcattcatat
     4861 ggtgagcgat ggctttgctg taaacggagt tcttatatgg tattacaaca tatgtaaaag
     4921 acaagtctgg cttatggctc ataatatcct tcctgacgaa tcgcatgatg atattcaggt
     4981 aggcaggttt atacatgaaa atactttcaa gagaaacgac aaagaagtat attttggcaa
     5041 tgtacgattt gatgttgttt tccaatccga ggaaaaaata gtgattggag aaactaaaaa
     5101 aagttcatgg aataataaag gatcaaaata tcaacttctt ttttatctta agcttcttaa
     5161 agaaaacggt attgaggcat caggtgttct tttgtaccca gaacagaaga aaagagaatc
     5221 tatagagctt acatctgaaa atatcttgga ggttgaaaga atgcaaaaag agataatagc
     5281 gttaatttca gaagataaac cactcccagc tgaaaaaaac ttatactgta aaaactgtgc
     5341 ttacagtgaa tattgctatg cttgaatatg aaaaaggata tctatatatt taaaagtggg
     5401 gaacttaaaa gaaaagataa tacacttttt tttgaaagcc aggaagggaa aaaattcata
     5461 cctattgaag aaacagacaa tatatacgta tttggggaag ttgatataaa taaaagattt
     5521 cttgattatg ccggagaaaa agaaatatgc cttcattttt ttaactatta cggatactac
     5581 agtgggtctt actatccaag acaacatctc aattcaggat ttgtaacgct caagcaggca
     5641 gagtactatc ttgattatga aaagcgtgtt gtgatagcca aaaacataat tcttgcagca
     5701 ttaagaaaca tacagatagt attgaggtat tacaacaaga acaaagaaga tttcaactca
     5761 acaattgaaa taataaaaga aaacatgctt acaataaaag aaagcaaaac aatagaagaa
     5821 ataatggctc aggaagcaaa cagcagaata gcgtattata aggcatttaa taatataata
     5881 aaaaatccag atttttactt tgaaaaaaga actaagagac cgcccaaaga caagataaat
     5941 gcactcataa gttttggaaa ctcaatgatg tacgttacag ttctttcaga aatatataaa
     6001 acaaatcttg atccgaggat aggatatctt cattctacaa acgaaagaag atttacgttg
     6061 aatctagata tagctgaagt ttttaagcct ataatagttg acagagaaat attttcattg
     6121 ctaaataaaa acattttaac taccggggat tttaaggaag atcttaacgg aacgatttta
     6181 aaagaagatt ctatgaaaaa atttgtacag gcttacaatg aaaagcttat gacaaccata
     6241 aatcactata aacttaatag agaagtatca tataaaagac ttatcagaat ggagctttac
     6301 aaattacaga aacacattac cggggaagcc acgtatgaag gatttacagc cgaatggtga
     6361 ggtgaaaaat gtttgctatt cttgtttacg atttcaatga aaaaagagta ggaaaagcat
     6421 taaaaaaatg cagaaagtat cttaactggg tacaaaattc tgttttcgag ggagagatta
     6481 caagaggaaa tttaacaaaa ctaaaaatgg aactacagtc tataatgaaa aaagatgaag
     6541 attcaataat aatatataca atggaaaata cgaaatactc tgaaagagag ataataggtc
     6601 tggaaaaaaa tgaaatatca caaataattt gatctgtcga ccataagtgt ggtaaaatat
     6661 ataggagtac gacagaattt gaaaatagga ttttttaagc tttgcaagga ataagaaaca
     6721 ataaggaaac aagatggcaa tttatataaa tcttgtttat aagattactt gaacgtcttc
     6781 ctagcatacc tataaggttt tgaaacaaac tgtaattcca gcaaacctac cgtcgtgcct
     6841 gccttcctag catacctata aggttttgaa actgatct
//
LOCUS       DAOSHR010000110         6876 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_9609_length_6876_cov_5.094121, whole genome shotgun sequence.
ACCESSION   DAOSHR010000110 DAOSHR010000000
VERSION     DAOSHR010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 6876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6876
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9609_length_6876_cov_5.094121"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..334)
                     /locus_tag="PLU28_06970"
     CDS             complement(35..334)
                     /locus_tag="PLU28_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HOG34839.1"
                     /translation="MENYSIVEALKMVLTSGNMLAWGLYYMGKFIGTGICMGIGAIGP
                     GVGEGETGAHAMDAMARQPEMVGSIRINMLLAMAVAESTGLYSLVIALIMLFVLP"
     gene            complement(362..1192)
                     /gene="atpB"
                     /locus_tag="PLU28_06975"
     CDS             complement(362..1192)
                     /gene="atpB"
                     /locus_tag="PLU28_06975"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HOG34840.1"
                     /translation="MINEKVTKYLIIVFLSLYIVLGIVNLFVFNSELSGVGQRWIVSI
                     NGKTGFMNEVNPMTLIMSFAIMIGLIIFASTVKFRLVPEKKRQTVFELLFEFYWGIVE
                     DAVPNPKYRKPVFIIGVSFFLYVLVSNLLGGMPGINVSPLSNGKVSVEMFTDTWYTPT
                     SDLNVNAAYAISVLIISHVFSAHSKGVWKWFKGFFEPIIFLLPLNIIGEIAKPISHSV
                     RLFGNIMGGGILVLIVSYMLKYFLIPVFLWGFFGWFVGLIQAFVFSLLAIAYIGSAVE
                     "
     gene            complement(1204..1542)
                     /locus_tag="PLU28_06980"
     CDS             complement(1204..1542)
                     /locus_tag="PLU28_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34841.1"
                     /translation="MRDLERKMLEMIKKIALLSVAEFLLLVFFFKLYSFWVFWGSFGA
                     ILGIMLMVEDIKKMPYVKWKKIPLGYLIRYFVYGIILTVGALVDVRALMISFVGIMNM
                     KIVPFLSYKQ"
     gene            complement(1478..1756)
                     /locus_tag="PLU28_06985"
     CDS             complement(1478..1756)
                     /locus_tag="PLU28_06985"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HOG34842.1"
                     /translation="MKSFDPKIFTQSNSVLNFALIVLSNIFVSFFIGQWISSLTKSVV
                     WTIVFLFLGVISGLYCGIKELIREAEKYEGSGKKNAGDDKKDSSSFRG"
     gene            complement(1753..2220)
                     /locus_tag="PLU28_06990"
     CDS             complement(1753..2220)
                     /locus_tag="PLU28_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HOG34843.1"
                     /translation="MLDVKIINATLDALKVAFVTAAKTDFKVQKPLMVKEVERIYDVI
                     TVIGFNGAIDGNLVYAVKREGAITIVNNMMSGMLEINSLDEMALSAISELGNMVSGSI
                     AMSLEKIGYSINITPPSVITGKDLQVLSEGTILKFPGIIHDETEMGVFLAMKT"
     gene            2377..2898
                     /gene="amrA"
                     /locus_tag="PLU28_06995"
     CDS             2377..2898
                     /gene="amrA"
                     /locus_tag="PLU28_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HOG34844.1"
                     /translation="MIGEHSYVKWAIKAIETYVLSEMKAQPSSDLPEDLFVRTAACFV
                     SLHTISGELRGCIGTIEPVYNSLCEEILENAISAATKDPRFPPLSKNELDSIKVNVDI
                     LSSLEKTDSASSLNPKIYGVIVKSGWKKGVLLPDLEGVDTVEQQLRIAKLKAGILGNE
                     KIELYRFTVQRYL"
     gene            2900..3898
                     /gene="amrS"
                     /locus_tag="PLU28_07000"
     CDS             2900..3898
                     /gene="amrS"
                     /locus_tag="PLU28_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOG34845.1"
                     /translation="MKVSALNYTETKDLGIKCGLCPHNCLIAENQTGFCLVRKNISGE
                     LIAQSYANVSSVAVDPIEKKPLYHFFPASKILSLGSWGCNLKCPFCQNFEISQYCRNE
                     GKYIYPEMLGELILKTGVRSVAFTYSEPIVWYEYVLDCCRELKKAEERFYTVMVTNGY
                     INKEPAKLLLNYLDALNIDLKVFNSQKYRNILQGGLKEVLDFIELSVKMKVHTEITTL
                     IVPGLNDDISELSEEFRWISSLSKNIPLHISQYFPRYKYEAEPKSYNFLVKVYQTAKE
                     YLDYVYIGNTPVWEYSDTYCPDCKTLIVKRKGYDVKVYNADEDGGCKKCGRKILVV"
     gene            3871..4917
                     /locus_tag="PLU28_07005"
     CDS             3871..4917
                     /locus_tag="PLU28_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOG34846.1"
                     /translation="MRTKDFSSISKSTVLIFSMLGISLILLLFIIFSKPLPTYEKSEI
                     FSLGTYVRIYIAGGKVSSKSLLKIAEKEIERLYAKYSSNIPSSVVYQLNEKREIECDE
                     ESLFLFQAAVNVSKITGDSFDPTLRPVLMLWGFDDVNSSKRVPSSYEIEQVLPFVSYK
                     FIDIDSKNRKIKLLKEGVTVDLGGISKGFIVDRVIDRIKEIDPKATGFIDAGGDIGII
                     GPKFGNRPWQIGIRDPFSSDPAKLIDTLYIYSGAVATSGNYERYFIQNGTLYHHIIDP
                     KTGYPANNCRSCTVISDKVMTADAYATGLFVLGIDNPALEYFTKFGVQAYLIDNTGKT
                     VETSGFEYFRNKVK"
     gene            4920..5297
                     /locus_tag="PLU28_07010"
     CDS             4920..5297
                     /locus_tag="PLU28_07010"
                     /inference="COORDINATES: protein motif:HMM:NF018685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HOG34847.1"
                     /translation="MAKRTKKYDIFIVAAILLFAVIMIFLNLLIPQKGIIGVKVTIDG
                     VLAVSISQDGLYPVEKDGKHLLTVEFYQGKARVKESTCDNQICVKTGWISNTNQTIVC
                     LPYKIVVEPIGGKNTDGVDIITW"
     assembly_gap    5358..5457
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5458..5961
                     /locus_tag="PLU28_07015"
     CDS             <5458..5961
                     /locus_tag="PLU28_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HOG34848.1"
                     /translation="KKLSNISILTGIACAMYVVESFIPLPMPLPGVRWGFSNLPVLLA
                     AVLGYGILPCAMIAFGKSFIGALLSGRIASATFFMGLSGSLASALFMGWLKKYVPKVG
                     TIGISELGALLNNTVQILIAGLILGNSRAVFYYYPYLVLIGTVTAYLLAVVSDIFLRS
                     MNHDAKV"
     gene            5945..6520
                     /locus_tag="PLU28_07020"
     CDS             5945..6520
                     /locus_tag="PLU28_07020"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HOG34849.1"
                     /translation="MTLKYRLVLGSGSARRKELLKFISNDFRITVSDIDEECSQSLPE
                     LYVQEVALNKSKALKIENDELLITADTIVFIEDKFLGKPRNREHAFQMLKKLSGAAHK
                     VYTGVCLRTNLFTDVFYECTEVFFRELSDNEIYYYIDAFSPYDKAGAYGIQDFGLVFI
                     QGIKGDYYNVMGFPVTSVYKKLLERGIIKYE"
     gene            6513..>6876
                     /locus_tag="PLU28_07025"
     CDS             6513..>6876
                     /locus_tag="PLU28_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HOG34850.1"
                     /translation="MNDEHQLPREKLETHGVQALSDEELIAVILRSGTKNASVFKASR
                     DLMERYKDLITLADAGISDIASVQGIGRVRAINLKASLEIGKRYHLQKKKAGTHYKMD
                     SPDNVYTFSEEMIHYDKEV"
BASE COUNT         2217 a         1299 c         1311 g         1949 t
ORIGIN      
        1 gatctcctcc tttcaggaag atttccttcc ttttttaagg aagaacaaaa agcattataa
       61 gagcaataac caatgaataa agacctgttg attctgcaac ggccatagcc aaaagcatgt
      121 ttattcttat agaaccgacc atttcaggtt gtcttgccat agcatccata gcatgagctc
      181 ctgtctctcc ttcaccgact ccaggaccga tagctcctat acccatacat attcctgttc
      241 ctataaactt acccatataa tataatcccc atgcgagcat gttgccgctt gtcaaaacca
      301 tcttcaaggc ttctacaatg ctgtaatttt ccataactta tctgacctcc cttagtcttt
      361 tttattctac tgctgaacct atatatgcta ttgccaacaa agaaaaaacg aatgcctgta
      421 tgagaccgac aaaccatccg aagaatcccc agagaaaaac agggataaga aagtacttaa
      481 gcatataact gactataagt accagtattc ctccgcccat tatattaccg aaaagcctta
      541 ccgaatgtga tatgggcttt gcgatttctc cgatgatatt taacggaaga agaaaaatta
      601 tcggctcgaa aaaacccttg aaccatttcc atacgccctt ggaatgggca gaaaatacat
      661 gactgataat aagcacagaa atggcatacg cagcattcac gttcagatcg gaagtgggag
      721 tataccatgt atcagtaaac atctctactg atacctttcc gttggaaagc ggcgaaacat
      781 ttattccggg cattcctcct aaaagatttg aaaccaatac ataaagaaaa aaagaaacac
      841 ctattataaa aactggcttt ctgtacttgg gattgggaac agcatcttca actatacccc
      901 aatagaattc aaaaagcagt tcgaagactg tctgacgctt tttctccggt acaagtctga
      961 actttactgt actggcaaaa attatcagcc cgatcataat agcaaaagac attatcagag
     1021 tcatcgggtt gacttcattc ataaagcctg tttttccgtt tatggaaaca atccacctct
     1081 gacctacccc ggagagttcc gagttgaaaa caaaaaggtt aactatccca agaacgatat
     1141 agaggcttag aaatactatg ataaggtact ttgttacttt ttcgttaatc aaagaaatca
     1201 cccctactgt ttgtatgaaa gaaaaggaac tatcttcatg ttcatgatgc cgacaaaaga
     1261 aatcatcaag gcacgaacat caaccaaagc cccgacagtt aagattatac cataaacaaa
     1321 gtatctgatt aaataaccca gagggatttt tttccatttg acataaggca tcttcttaat
     1381 atcttcaacc atgagcatta taccgagtat ggctccaaag ctcccccaaa aaacccaaaa
     1441 ggaatagagc ttaaagaaga acacaagaag aagaaactca gccacggaaa gaagagctat
     1501 cttttttatc atctccagca tttttctttc cagatccctc atacttttca gcctctctta
     1561 taagttcttt tattccgcag taaagacctg atattacccc taaaaaaaga aatactatag
     1621 tccatacaac acttttggtt aaggaagata tccattgacc aataaaaaaa ctgacaaaaa
     1681 tgttggaaag tactatcaaa gcaaagttta atacgctgtt agactgtgta aaaatttttg
     1741 gatcaaagct tttcatgttt tcattgccag gaaaacaccc atctctgttt catcatggat
     1801 tataccggga aacttcagaa tagttccttc cgaaagaacc tgaaggtctt ttcctgtgat
     1861 aacagacgga ggagtgatat tgatactgta gcctatcttt tcaagactca tggctattga
     1921 gccggaaacc atattaccca gttcgcttat ggctgaaagc gccatttcat caagactgtt
     1981 gatctcaagc atccctgaca tcatattgtt tactattgta atggctccct cacgttttac
     2041 cgcataaaca agatttccgt ctatagctcc gttgaaacct atgaccgtaa taacatcata
     2101 gatcctttca acttctttta ccataagcgg tttttgaact ttgaaatctg tctttgccgc
     2161 tgttacaaag gctaccttca aagcatccag agttgcgttg attatcttaa catcaagcat
     2221 aagctcacct cctcatacct taaattatat aataaatgaa tttaattatc aataagcaat
     2281 cattatattg caacatgggc ttttgcatta catggaagga agacggactt tatgctataa
     2341 ttatataaat atccttttcg ctgtggaggg attgacatga taggagagca ttcttatgta
     2401 aaatgggcaa taaaagctat tgaaacttat gtgctgtcag aaatgaaagc acagccttct
     2461 tctgatcttc cggaagacct gttcgtaaga actgccgctt gctttgtttc actgcatacc
     2521 ataagtggcg aactaagagg atgtatagga accatagaac cggtatacaa cagtctttgc
     2581 gaagaaatac tcgaaaatgc tatatcagcc gccacaaagg atcctaggtt tccgcctttg
     2641 tcaaaaaacg agctggacag tataaaggtc aatgtagata ttctttccag cctagaaaaa
     2701 accgattccg catcctcact taatccaaaa atatatggtg ttatagttaa aagcggatgg
     2761 aaaaaaggag tgcttctgcc ggatcttgaa ggtgttgata ccgttgaaca gcagctgagg
     2821 attgcaaagc tcaaagccgg aattctcgga aatgaaaaaa tagagttata ccgttttact
     2881 gtccagaggt atctatgaga tgaaggtttc tgcgcttaac tatacagaaa caaaagattt
     2941 gggtattaaa tgcggccttt gtcctcataa ctgcttaatt gcagaaaacc agaccggttt
     3001 ttgcctcgta agaaaaaata tttccggaga gcttatcgca caaagttatg cgaacgtatc
     3061 ttctgttgca gttgatccta tcgaaaaaaa accgctttat cattttttcc cggcaagtaa
     3121 aatactttct ctgggaagct ggggctgcaa tttaaagtgt cctttctgtc agaattttga
     3181 aatatcccag tactgccgca acgagggtaa gtacatatac ccagaaatgc ttggggagct
     3241 tatcttgaaa accggtgtaa gatccgttgc ctttacttat tcggaaccca tagtatggta
     3301 tgagtatgtt cttgactgct gccgtgaatt gaaaaaagca gaggaacggt tctataccgt
     3361 catggtgacc aacggttata taaataaaga acccgcaaag ctgcttttaa attatcttga
     3421 cgctttaaat atagatttaa aggttttcaa tagtcagaaa taccgtaata ttttgcaggg
     3481 cggccttaag gaagtcttag attttattga gctttctgtc aaaatgaagg ttcatacaga
     3541 gataactact cttatagttc cgggactgaa cgatgatata tccgagcttt ccgaggaatt
     3601 cagatggata tcgtctcttt ccaagaatat ccccctgcac atcagtcagt acttcccgag
     3661 atataaatat gaggctgaac ccaaaagtta caattttctg gtaaaggtat accagactgc
     3721 taaagagtat ctggattatg tctatatagg caatacacct gtctgggagt acagcgatac
     3781 atactgtcct gactgtaaga cacttatagt aaaaaggaaa ggctacgatg taaaagtgta
     3841 caatgcagat gaagacggag gatgcaaaaa gtgcggacga aagattttag tagtataagc
     3901 aaaagtactg ttttaatttt ttcaatgctg ggaataagtt taatcctttt attattcata
     3961 attttttcaa aaccacttcc cacttatgaa aagtcagaaa tattttcctt gggcacctac
     4021 gtaagaatct atatagccgg aggaaaagta tcttccaaaa gccttttgaa aattgctgaa
     4081 aaagaaatcg aaagacttta tgccaaatac agttcaaata taccttcaag tgtagtatac
     4141 cagttgaatg aaaaaaggga gatagaatgt gacgaagaaa gtcttttcct ttttcaggct
     4201 gcggtaaatg tatccaaaat cacgggtgat tcttttgatc ctactctaag accagttctg
     4261 atgttatggg gctttgatga tgtaaactcc agtaaaagag ttccatcctc ctatgagata
     4321 gaacaggttc tcccgtttgt cagttacaag tttatagata ttgacagcaa aaacagaaaa
     4381 ataaagctgc tcaaggaagg tgtgacggtt gatcttggcg gtatctccaa aggttttatt
     4441 gtcgacaggg ttatagaccg cataaaagag attgatccta aagccacagg ctttatagat
     4501 gccggaggag atataggaat aataggacct aaattcggta acagaccatg gcagataggc
     4561 ataagagatc ctttttcctc tgaccctgca aagcttattg atacgcttta catatatagt
     4621 ggagcagttg ctacttccgg aaactacgag agatatttca tacaaaacgg tacgttgtat
     4681 caccatataa tagacccaaa aacaggctat cctgcaaata actgcagaag ctgcacggtt
     4741 atttcagata aggttatgac agcagacgcc tatgctaccg gactttttgt actaggcata
     4801 gataatccag cactggagta ctttaccaag tttggtgtac aagcatatct tatcgataat
     4861 accggaaaaa ctgttgaaac cagtggtttt gagtacttca gaaataaagt gaagtagtta
     4921 tggcgaaaag aacaaaaaag tacgatatat ttatagtagc agctattttg ctttttgccg
     4981 taattatgat ttttttaaat ttattaatac cacaaaaagg aataattgga gtaaaagtca
     5041 ctattgatgg tgtactggct gtttcaatat ctcaagatgg actttatcct gtagaaaaag
     5101 acgggaaaca ccttcttaca gttgagtttt accagggaaa ggcaagggta aaggaatcca
     5161 cctgcgataa ccagatatgt gtcaaaacag gatggataag caacacaaat caaactatcg
     5221 tttgccttcc gtataagata gtagttgaac ctataggcgg aaaaaacaca gatggagtag
     5281 atattataac atggtaaaat cgaaaaaact ttcaaatata agtattctta caggtattgc
     5341 ttgtgccatg tacgttgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaaa
     5461 aaactttcaa atataagtat tcttacaggt attgcttgtg ccatgtacgt tgtggaaagt
     5521 tttattcctc ttcccatgcc gctcccagga gtaagatggg gattttcaaa tcttcctgtt
     5581 cttttggcgg ctgtactggg ttacggaatt ttgccctgtg caatgattgc ttttggaaaa
     5641 agttttatcg gagctcttct gtcaggaaga atagcttcgg caacattctt tatggggcta
     5701 tccggatcac tggcaagtgc cctttttatg ggatggctta agaagtatgt tccgaaagtt
     5761 ggaaccatag gaataagtga gttgggtgcg cttttaaata atacagtgca gatacttatc
     5821 gcagggctta ttctgggtaa cagcagagcc gtattctact attatccgta tcttgtttta
     5881 ataggtactg ttaccgcata cttgcttgcg gtagtttcag atattttttt aaggagtatg
     5941 aaccatgacg ctaaagtata gattagtgct gggttcagga tcagccaggc gaaaggaact
     6001 tcttaagttt atttcaaatg atttcagaat aactgtaagc gatatagatg aagagtgttc
     6061 ccaaagcctt cctgaacttt acgttcaaga agtagctctt aataagagta aggctttaaa
     6121 gatagagaat gatgaactac ttatcactgc agataccatt gtgtttatag aagataaatt
     6181 tctgggaaaa ccacgaaatc gtgaacacgc atttcaaatg ctgaaaaaac tttcaggagc
     6241 tgctcacaag gtctacacag gagtctgtct gagaactaat ctatttactg atgtttttta
     6301 cgaatgtaca gaggtttttt tcagagagct tagtgacaat gaaatatact attatataga
     6361 tgctttttct ccttacgata aagccggtgc ttatggcata caggacttcg ggcttgtttt
     6421 tatccaaggt ataaagggcg attattataa cgttatgggg tttccggtta catcagtcta
     6481 caaaaaactg ctggagcggg ggataataaa gtatgaatga cgaacaccaa cttccacggg
     6541 aaaaacttga aacacatggc gtacaagctc tttctgacga ggagcttatc gcagtgattc
     6601 ttaggagcgg aaccaaaaac gcatctgttt ttaaagcttc aagagatctt atggaaagat
     6661 acaaagatct tattaccctt gcggatgcag gtataagcga tatcgcatca gtacagggca
     6721 taggcagggt aagagccata aacctcaaag catctctgga aataggaaaa agatatcacc
     6781 ttcagaaaaa gaaggccggc acacattaca aaatggactc tcccgataac gtatacacct
     6841 tttcggaaga gatgatccat tacgacaaag aagtgg
//
LOCUS       DAOSHR010000111         6875 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_9610_length_6875_cov_5.105572, whole genome shotgun sequence.
ACCESSION   DAOSHR010000111 DAOSHR010000000
VERSION     DAOSHR010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 6875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6875
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9610_length_6875_cov_5.105572"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..920)
                     /locus_tag="PLU28_07030"
     CDS             complement(<1..920)
                     /locus_tag="PLU28_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HOG34851.1"
                     /translation="MTNLVAIGGGTGLSQLLRGLKWYEELSIVSIVAVTDDGGSSGII
                     RQDFEIPPPGDVRNNIVALAEKESLITDLLSYRFEEGFLGGHNLGNIILLALTRINGN
                     SFPLAVKSLSDFLNTKGRVLPCSCDMLRLVASFENGDVVFGETSINRHGGKIKEVWLD
                     TVAEPFSETIASLEKADHIVLGPGSLYTSIITNFLVREVKETIKKSGAKLIYISNIMT
                     QPGETENYTLSQHVSQIEYYMGRKIDKIVVNDSSFPSDVLERYKEKNSVPVLQDMCDS
                     RIITGDIAYIINREKPIIRHDSNKLAKIIVS"
     gene            complement(926..1783)
                     /gene="rapZ"
                     /locus_tag="PLU28_07035"
     CDS             complement(926..1783)
                     /gene="rapZ"
                     /locus_tag="PLU28_07035"
                     /inference="COORDINATES: protein motif:HMM:NF015620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HOG34852.1"
                     /translation="MKENPTIYLITGLSGAGKTFLLKAMEDEGYYTVDNVPPHLIKDM
                     INIICTSEYSKVAVVSDIRWKNTKDLIDVFLNIESYVNQSVNIKKVFLYADKQILMKR
                     FMQSRRGHPLGVPTEEAIEKEMQLLNPIKEICDIVLDSSNTEPSQLRKRFFEIINEGK
                     RTLKLNIVSFGFKYGYVENSDYVFDVRYLPNPYYIFEMYKLTGLDHQIGEYLENFEQT
                     GETVRRLFDFACFVQDNYSESGRIEAYFCIGCTGGKHRSVYIAQRIYELMREQNREVS
                     IFHRDMIKE"
     gene            complement(1798..2592)
                     /gene="murI"
                     /locus_tag="PLU28_07040"
     CDS             complement(1798..2592)
                     /gene="murI"
                     /locus_tag="PLU28_07040"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HOG34853.1"
                     /translation="MRVGFFDSGLGGLTVLKKVVANFGNHQYFYFGDTLRVPYGSKPI
                     GYLAELLEKTLDFFEDLNIDILISACNTSDSLVKNRIVNVGNRNFRYISIIDNGIRQI
                     SANERVLLLATENTVKSGSYRDGLVEKGIGKLTEKSCPLFVPLIEEGFWYGQMAESIV
                     RYYLEDSNSGFDKIILGCTHYPILKKHIIKNITAEIIDPADGIIKELGTSYNLKHSSG
                     LPKINFYITGDVEKFKVLSGRFLRTVYFRGYYFKMDLETKKYYYFR"
     gene            complement(2617..2901)
                     /locus_tag="PLU28_07045"
     CDS             complement(2617..2901)
                     /locus_tag="PLU28_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34854.1"
                     /translation="MSKSYRDVQIEILDKIYRYKVDESSDMIDPVLLEIKNEVEKYID
                     KVGKSKLDEVLVLLLLNEKLENMKTKSQLDGLVRKIGDIVDPIQSYTEER"
     gene            complement(2980..3261)
                     /locus_tag="PLU28_07050"
     CDS             complement(2980..3261)
                     /locus_tag="PLU28_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34855.1"
                     /translation="MMESLKEIEQLIETLSGKYNSLKAEQNKVKEEKEKALADYRELF
                     VEYEKVCKEKEDMQRVIDSLRLKGEEAETRLQQIKKSIMDKINAEQTEG"
     gene            complement(3332..3856)
                     /locus_tag="PLU28_07055"
     CDS             complement(3332..3856)
                     /locus_tag="PLU28_07055"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOG34856.1"
                     /translation="MFLGDKVKLRAYKKEDVPLACEYINDPEVSLMLSPLIPFPYKLE
                     DEYKWYDSLGMGRDGKYNFAVERVSDGLYIGGCGINDIDWKNSVATVGIFICAKECGK
                     GYGTQAMGLLIDFIFSQMNIHKIKLNVFSFNHRAIKSYEKLGFKKEGVLRQEIFRNGE
                     YFDEIVMGLLKDEH"
     gene            complement(3870..6530)
                     /gene="polA"
                     /locus_tag="PLU28_07060"
     CDS             complement(3870..6530)
                     /gene="polA"
                     /locus_tag="PLU28_07060"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOG34857.1"
                     /translation="MKDLYLIDGSGIAYRAFFAVDQNLTAPSGLHTNAIFGVCRMMLK
                     IIKDYIKKDEDGILFVFDKKTKTYRHELLESYKAQRPKAPAEFVEQLGYIVRLVELLG
                     INVACVDNYEADDVIATVAQRYKEQVENVYIITSDKDMMQLVKDNIFILRPEKGIGEV
                     VKFDEEKIFQKMGVPPSKIIELLALMGDASDNVPGVRGIGEKTAQKLLQSYKDLDDLY
                     QRIDELSASTKEKLLSDRDMAFLSRKLVQLSKDVPLYYKDGELLYSHFDEKLKDFLQE
                     LGFKSMIREVGDTPRKSSDSECKGVCEEYTLDRSREFFARAGNTHELSVSVSAWPKDP
                     FKGVLKGAALSWKDNGDTYTSYMDLSGAGEGEAEDFLKQLSVLFEDKVIIGYNLKFLL
                     SVFLLHGLKEFKVGFDTMTGAFLLMPDEKRFDLDSICQKYLGMKMPQVQEPSRSTLFD
                     GQGQSGEDETLKKACLAESLYNLLLFDELSKLIKEKELESAAYDIDFPVTNVLAFMET
                     KGVYFDTQYLKILENEFTAKINDIDFQIRKYSDQAFNPNSPKQVGELLFGKLGLKGTK
                     KTKTGSFSTDAETLEEISDQHPVIPLILGNRKYQKLLSTYINAIPALVNPITGRVHTS
                     LNITGAATGRLSSTEPNLQNLPIREEEGEKIRGAVKAQKEDSVIISADYSQIELRVLA
                     HMSGDEVLIDAYHHDKDIHALTASKIFGIDLEQVTSAERRVGKTVNFSIVYGVTAFGL
                     ANRTGISNLQAQRFIDKYFDLYRGVDLYQRNILNFLRINGYVQTIFGRKRFLRNMPFN
                     KGEIERMAINSPVQGSAADIMKLAMIKLYKTLPQYAQMILQVHDEILIEVPQKMAEQV
                     VPVICNAMENAVELKVPLKVDVNISKNWKK"
     gene            complement(6527..>6875)
                     /locus_tag="PLU28_07065"
     CDS             complement(6527..>6875)
                     /locus_tag="PLU28_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34858.1"
                     /translation="AYKHKLIELKDKLARKLPFFRGSELSHKGAASDALSSLEEVLGE
                     QIKELTPSQLVELRKALTYDVPAFFDDNEKLKGILDDAAKEKMKKLITDMKNKSFPVP
                     SSSVENKWKEDEK"
BASE COUNT         1939 a         1576 c         1086 g         2274 t
ORIGIN      
        1 gagactatta ttttcgcaag tttattagaa tcatgcctta ttataggctt ttcacgattt
       61 attatataag caatatcgcc tgtgattatt ctactgtcac acatatcctg taaaaccgga
      121 actgaatttt tttctttata cctctcaagc acatccgaag gaaaggaaga atcgtttacc
      181 actattttgt ctattttacg acccatatag tattctatct ggcttacatg ctgacttagg
      241 gtatagtttt ctgtctctcc gggctgagtc attatattgg atatgtatat aagctttgct
      301 ccggattttt ttatcgtttc ttttacctct cggacaagaa aatttgttat tatgctggta
      361 tacaatgaac ccggaccaag aactatatgg tcagcttttt caagtgaggc tatggtttct
      421 gaaaatggtt ccgcaacagt atcaagccac acttccttga tctttcctcc atgacggttt
      481 atactggttt cgccgaaaac cacatcacca ttctcaaaag atgccaccag tctgagcata
      541 tcgcaagaac agggtagtac tctaccctta gtattcagaa aatctgataa actttttacc
      601 gctagcggaa aactgttacc gtttatcctt gtaagagcca agagaataat atttccaaga
      661 ttgtgtcctc caaggaaacc ctcttcaaat ctgtatgaaa gaagatcagt tataagtgat
      721 tccttttccg caagtgccac tatgttgttt ctgacatcgc cgggcggagg aatttcaaaa
      781 tcctgcctta ttatcccgga acttccgcca tcatccgtaa cagctactat cgaaactata
      841 ctgagctctt cataccattt gagccccctc agaagctggc ttaatcctgt tcctcctccg
      901 attgcaacaa ggttggtcat tcatatcatt ccttaatcat gtctctgtga aaaatactta
      961 cctctctatt ctgttctctc atgagttcat atattctttg ggctatataa acggacctgt
     1021 gctttcctcc ggtacagcct atacagaaat aagcctcaat ccttcctgac tctgagtaat
     1081 tgtcctgcac aaagcaggca aaatcaaaca acctgcggac agtctcacct gtctgttcaa
     1141 aattttcaag atattctcct atttggtgat ctaaccctgt aagtttatac atttcaaaaa
     1201 tataatacgg attaggaaga tatctcacat caaaaacata atcgctgttt tcaacataac
     1261 cgtacttaaa tccaaagctc actatattca gtttaagagt tctttttcct tcatttatta
     1321 tctcaaaaaa acgctttctg agctgtgatg gttccgtatt ggaagaatca agtactatat
     1381 cgcatatctc ctttatagga tttaaaagct gcatctcttt ttctatagcc tcttctgtag
     1441 gtactcccaa aggatgtccg cgtcttgact gcatgaatct tttcataaga atctgcttat
     1501 ctgcatataa aaatactttt ttaatattca cggactgatt gacataactc tctatattaa
     1561 gaaaaacatc tattagatct tttgtgtttt tccatcttat atcacttacc acagcgactt
     1621 ttgaatattc gctggtgcag ataatgttta tcatgtcttt tatgagatga ggcggaacat
     1681 tgtcaactgt atagtatccc tcatcttcca tggccttaag caaaaaagtc tttcctgcgc
     1741 cggaaagccc cgttataaga tatatggttg gattttcctt cactagatca cccgctgtta
     1801 tctgaaataa taatactttt tggtctctag atccatttta aagtaatagc ctctgaaata
     1861 aaccgttctc aagaacctgc ctgaaagaac cttgaacttt tcaacgtccc cggtaatata
     1921 gaagtttatc ttgggaagac cgctactgtg tttaagattg taagaagtac caagttcttt
     1981 tatgattccg tctgcaggat ctattatctc agcggtaatg ttttttatga tatgtttttt
     2041 aagaatagga tagtgtgtac atccaagaat aatcttatca aatccgctgt tggaatcttc
     2101 aagataatac ctgactatcg attccgccat ttgaccgtac cagaaccctt cttctataag
     2161 agggacaaaa agaggacatg atttttcagt aagcttgcct atcccctttt ctaccagacc
     2221 gtctctgtag ctgccggatt ttacagtgtt ttctgttgct aaaagaagaa ctctttcatt
     2281 agccgatatc tgtcttatac cgttatcaat gatgcttata tatctgaaat tacggtttcc
     2341 aacattgact atcctgtttt taacaagcga atcggatgtg ttgcaagctg aaataagaat
     2401 atctatgttc agatcttcaa aaaaatccaa ggttttttct aaaagttcag ccagatagcc
     2461 tataggtttg gatccgtagg gcaccctaag ggtatcacca aaataaaaat actggtggtt
     2521 cccaaagttc gcaaccactt ttttcagtac ggttaatcct ccaagccccg agtcaaaaaa
     2581 acctactctc attaatttca ctcctgtcaa gaaaggctac ctttcttcag tataagattg
     2641 aatggggtca acaatatctc ctatctttct taccaacccg tcaagctggg atttagtctt
     2701 catattttcc agcttctcgt taagaagaag aagaaccaga acctcatcaa gttttgattt
     2761 cccgacttta tctatatact tttccacttc gttttttatc tccaaaagaa ccggatcaat
     2821 catatcagat gattcatcta ctttataccg ataaatctta tctaatattt ctatctgaac
     2881 atccctatag gatttactca aatcagccac ccttccgatt attctatctc tcatttgtta
     2941 acatacagaa tataaaacat tactaatcat tctataaggt tatccttcag tctgctcagc
     3001 atttattttg tccataattg atttttttat ctgttgaagt ctggtttcgg cttcttcgcc
     3061 ttttaatctt aacgagtcta ttactctctg catatcttct ttttctttgc agaccttctc
     3121 atactccaca aaaagttccc tgtaatcggc aagtgctttt tctttttctt ccttcacctt
     3181 attctgttcg gccttgagtg aattatactt tcccgaaagt gtttctataa gctgttcaat
     3241 ctcttttaaa gactccatca tctatattcc cccttaatat caagatattt ttaacattgt
     3301 ttcagatcaa attataccac aataataaca attaatgttc atctttgagc aatcccatca
     3361 caatttcatc aaaatactcc ccgtttctga atatttcctg tctcaaaacc ccttcttttt
     3421 taaatccgag cttttcataa ctttttatag cacgatgatt aaagctgaaa acgttaagct
     3481 ttatcttatg tatattcatc tgagaaaaaa tgaaatcaat gagtagcccc atcgcctgtg
     3541 tcccgtatcc cttcccacac tctttggcac aaataaaaat ccctactgtt gcaacagaat
     3601 ttttccaatc aatatcattt attccgcaac cacctatata aagaccatca gatactctct
     3661 caactgcaaa attatacttt ccatctctgc ccattccgag tgaatcatac catttatact
     3721 catcctcaag cttatacgga aagggtatca aaggagaaag catcaaagat acctccgggt
     3781 cgtttatata ctcgcatgca agcggaacat cttctttctt atacgctctg agcttaacct
     3841 tatctcctaa aaacaaaaca ttctcctcct tattttttcc agtttttgga tatattgaca
     3901 tcaaccttga gaggaacctt aagctccaca gcattttcca tcgcattgca gataactgga
     3961 accacctgct ctgccatttt ttggggaacc tctataagta tttcatcatg aacctgaaga
     4021 atcatctgtg catactgagg aagcgtttta taaagtttta tcattgcaag cttcattata
     4081 tcagccgcac tcccctgaac aggagagttt atagccatac gttctatttc ccctttgttg
     4141 aatggcatat tcctcagaaa cctctttctt ccgaatatgg tctgaacata tccgtttatt
     4201 ctcaaaaaat tcagaatatt cctttgatat agatccacac cacggtatag atcaaaatac
     4261 ttatctatga acctctgcgc ctgcagatta ctaataccgg tacggttagc aagtccaaat
     4321 gccgtgaccc cgtaaactat cgaaaaattg accgtctttc cgactcttcg ctcggcagat
     4381 gtaacctgtt ccagatctat gccgaaaatt ttagatgccg tgagggcatg tatgtcctta
     4441 tcgtgatgat aagcatcaat aagaacttca tcaccggaca tatgtgccaa aactctaagc
     4501 tctatctgtg aatagtccgc actgataata accgaatcct ctttctgggc tttaacagcc
     4561 ccccttatct tttctccctc ctcttccctt atgggaagat tctgcaagtt aggctccgtg
     4621 ctgcttaatc tacctgttgc agcaccggta atatttaatg atgtatgtac ccttcctgta
     4681 atcgggttta ccaatgccgg tattgcatta atatacgtgg aaagaagctt ttgatacttc
     4741 ctgtttccaa gaataagcgg aataacagga tgttgatcgc ttatctcttc aagggtctca
     4801 gcatcggttg aaaaacttcc ggtcttggtc tttttggttc ctttaagacc aagctttccg
     4861 aacaaaagct cgcccacctg cttgggagaa tttggattaa acgcttgatc agagtatttt
     4921 cgtatctgaa agtctatatc gtttattttg gctgtaaact cattctcaag tattttaaga
     4981 tactgtgtat caaagtacac accttttgtt tccataaatg ccagcacatt tgtcaccgga
     5041 aagtctatgt cgtaagccgc actttcaagc tccttttctt tgataagttt tgaaagctca
     5101 tcaaaaagaa gaaggttata aaggctttca gcaagacatg ccttttttaa ggtctcatct
     5161 tcacctgact gcccctgacc atcaaaaaga gtactacgtg aaggttcctg tacttgcggc
     5221 atcttcatgc ccagatactt ctggcatatg ctgtcaagat caaatctttt ttcatcaggc
     5281 attaacaaga aggctccagt catagtatca aaaccaactt tgaattcttt taacccatgc
     5341 agcagaaaca cagacagcaa aaatttaaga ttatatccga tgataacctt atcttcaaaa
     5401 aggacagaaa gctgttttaa aaagtcttca gcctctcctt caccggctcc actaagatcc
     5461 atataagaag tatatgtatc tccgttatct ttccagctca gggctgcacc tttcaacacg
     5521 cctttaaacg gatccttcgg ccaggcagat acacttaccg aaagttcatg ggtattaccg
     5581 gcacgggcaa aaaactccct ggatctgtca agagtatatt cttcacatac acctttgcat
     5641 tcgctgtcgc tggactttct gggggtatct ccaacttctc ttatcatact tttaaatcca
     5701 agttcctgaa gaaaatcctt aagcttttca tcaaaatgac tgtataaaag ctctccatcc
     5761 ttataataaa gcggaacatc cttagaaagc tgaacaagct tacggcttaa aaaagccata
     5821 tccctgtcgg ataaaagttt ttcttttgta cttgcagaaa gctcatcaat tctctgatac
     5881 agatcatcaa gatccttata gctctgaaga agcttctgag cagttttttc acctattccc
     5941 cttactccgg gcacattgtc tgaggcatct cccataagtg ccagcagttc gattatcttt
     6001 gaggggggaa cacccatctt ctggaatatc ttttcttcat caaattttac aacttcgcct
     6061 atacctttct caggtctgag aataaaaata ttatctttta ccagctgcat catgtcttta
     6121 tcagatgtaa ttatgtatac attttcaacc tgttccttat acctctgggc aactgtcgca
     6181 ataacgtcgt cggcttcata gttgtctaca catgcaacat ttattcccaa aagctcaacc
     6241 aaacgcacga tatacccaag ttgctcgaca aattctgcag gagctttggg cctttgcgcc
     6301 ttatagcttt caagaagctc atgccggtat gttttggttt tcttgtcaaa aacaaaaagg
     6361 ataccgtcct catccttctt tatatagtcc ttgattatct tgagcatcat tctgcaaaca
     6421 ccgaagatag cattggtgtg aagccctgag ggggcagtca gattctggtc aactgcaaaa
     6481 aatgccctgt atgctatgcc agaaccgtct ataagataaa ggtccttcat ttttcatcct
     6541 ctttccattt gttttccaca ctgctgctgg gcacaggaaa actcttattc ttcatatcag
     6601 ttatgagttt cttcattttt tccttggcag catcatcaag aatccctttc aatttttcgt
     6661 tatcgtcaaa gaaagcaggc acatcataag taagggcttt cctaagctct acaagctgcg
     6721 agggggtaag ctcttttatc tgctcgccca aaacttcttc caatgaggaa agcgcatcgc
     6781 tcgcagctcc tttatgtgaa agctcgcttc ctctgaaaaa tggcagtttg cgtgcaagct
     6841 tatctttaag ttctatcagt ttgtgcttgt aagcc
//
LOCUS       DAOSHR010000112         6798 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_9752_length_6798_cov_5.800830, whole genome shotgun sequence.
ACCESSION   DAOSHR010000112 DAOSHR010000000
VERSION     DAOSHR010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 6798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6798
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9752_length_6798_cov_5.800830"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..831)
                     /locus_tag="PLU28_07070"
     CDS             complement(<1..831)
                     /locus_tag="PLU28_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34859.1"
                     /translation="MENNGSVDVVIEKLIYGGYGLARYNNEIYMVENVYPQDIVRVKE
                     KFKKKNTIFASVEQIIKPSPYRTASPCKHFPKCGGCQWLGLKYEQQLQAKHSIVEEQF
                     RRIGSIEVNVSQIIESDDSTQYRSKMEFGFSYGKKTVLGLKEASTHNIIDINNCIISP
                     APFDKIRNTVKSIISDMNLSVYDPEKKSGVLKHLVLRKSFSKNETMLIFVTHTEYFPK
                     EHEFKEELSKKVHADSIVHVMNTSDTFTLRGPYKTWKGEGVLREEFDGFLFQIPPTSF
                     F"
     gene            967..2208
                     /locus_tag="PLU28_07075"
     CDS             967..2208
                     /locus_tag="PLU28_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG34860.1"
                     /translation="MNKKEKSWILYDWANSAYSMAVTSTILPIFFKDVASKGLSDTDS
                     SAFWAYSNSIETVLLVLMLPILGTIADYKNMKKRFFIFFLLMGIFFTGLLSTVSEGQW
                     FFCLFIYVLTAIGFSGSNLFYDSMLVDVTTEDRMDWVSTNGFAYGYIGSTIPFIISIA
                     LIMKPQILGFDSAIIPTKLSFIITALWWFIFSIPLLKNVQQVNYLEPCTRPVKESFLR
                     LIKTFKEIKQYKNIFLFLLAFFFYIDGVHTIISMATIFGKDINLSNTTLMVVLLVIQF
                     VAFPFAIIYGKLAQKFSAKIMLFVGIIVYTFVTIYAYFIKSELEFWILGLLVATSQGG
                     IQALSRSLFGKIIPKEKSAEFFGFYNIFGKFAAIFGPLLYGMASQITGNPRFGVLSLI
                     ILFIIGGVLMIFVKAQSPSKS"
     gene            2219..3118
                     /locus_tag="PLU28_07080"
     CDS             2219..3118
                     /locus_tag="PLU28_07080"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOG34861.1"
                     /translation="MISISCLKIKGGSYYINSPTNIGLYVFRNKNCLVIDTPLNSSAA
                     KKIDEEISSCAFHPKYVFNTHHHLDHCGSNSYFRNKYPGCEFFSSKGEKVFIENNFLY
                     SSILWGGIPCSHIEWSTKEEKIDAILEEGINKINDEKFEIIDLGGHSKALTGIVTPEK
                     VFYISDSIFSEEKLQKYKIPYIYDIKKFLISVNKILSSDAEFFLPGHSCKVYDKQEIE
                     QLASKNVSAVEFFRDQIYTFLKNAFTKEEIVENIIILNELSVDFKQYMLISTTVAGYL
                     EMFISEKLISYSVQDGKLYFYRE"
     gene            complement(3120..3758)
                     /locus_tag="PLU28_07085"
     CDS             complement(3120..3758)
                     /locus_tag="PLU28_07085"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HOG34862.1"
                     /translation="MTVYDLTHVIEESMPVYPGTDGPRLSTACTLEKDGFKETLVTMF
                     THTGTHVDCPEHLFQSGESTDKKDISSFFGKGCVLDFSFLKENAVITKKYLLESEKTI
                     NASDFVILHTGWARFWNFPEYFGNFPVLDKQAAQYLSQFNLKGIGLDTISLDPVNHEL
                     INHKIILSKHICIIENLNNTEKLIGKEFLFACLPLKIKNGDGSPIRAAGIVL"
     gene            complement(3773..6265)
                     /locus_tag="PLU28_07090"
     CDS             complement(3773..6265)
                     /locus_tag="PLU28_07090"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOG34863.1"
                     /translation="MLRAKSLNKFVLTSTLFVFVSFIFLISIFLYISLNEYIKKEAVK
                     KAESAAMLTVSYVEKQFERALLNARFLSFLLETTKAQSNPSRDDVIKILKNIVENNSE
                     FLGAWVVFEPDAFDARDYEYINSPGADKDGRFVPYYNSIDGYHLESCYGYDDPSSFSD
                     WYNIPKRTGLETIVDPFEYTIKNETVLLISYSVPIFYNNTFIGVAGVDISNIQLKNFV
                     SGLNSVNDSKVFILSSTGKFMVHPDDTFHGKNFESFLSFRNTDEKKILAAMHSGKLNS
                     FFDYSYFESSITLNIIVPIKITKYASSITLFMTMNTDKLFYQSNKILNFVLMVLLVSI
                     IFSITFIFILSNRITSPIIELAEKVRAFGRGDFDISFESGRNDEIALISKELDKMSKA
                     LIQKMEEINQKNDLLNDKNQELASSYRSLQSKEMELRTLYETISKNKNILDEKNQELE
                     KLNIRLNELLRLETMSKERFMGFLNVTGEFSDILLLSDSQIIRRLMINTSSMLKNDYS
                     ISILFDEDLNIKDISTVGPKALNIDFINFTVKEIDIFDSVTKITPDEILASSKYGYMK
                     KLLKDHTSNVSDIIACPIKNGGKSFGQFYFFFFNRIFFSPEDIKIITSFQKITELLLK
                     IKIYADRINNAYTNFAHKLALIAENYDEDTGFHINRVGKIAGFLALKYGLPKETVRQI
                     EKFGPLHDIGKIFIPKELLMKKSKLSEQEYEVMKNHTLYAKNILQDDDYFTVALNIAL
                     YHHEKYDGSGYNSGLSGESIPIEAQLVSITDVYDALRSKRPYKDAMSHIHAVEILKNG
                     DSRTKPTDFNPKILELFIKYEREIGRIYDIFS"
     gene            complement(6320..>6798)
                     /locus_tag="PLU28_07095"
     CDS             complement(6320..>6798)
                     /locus_tag="PLU28_07095"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF013713.2"
                     /note="catalyzes the formation of spermidine from
                     putrescine and S-adenosylmethioninamine; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HOG34864.1"
                     /translation="PFTSCEFDNSKLDIVIEDGAKFVKKFHDYFDIILIDSTDPTEGQ
                     ASQLFKEDFYLSCLEALKKDGILSAETEDPFIHKEWMINAYKRIQNVFSNAYLYTAYI
                     PQYAPGCWSFTLAFKTPGSIEPKIQNHEILTKTRYYNLNIHKASFALPQFINTLIE"
BASE COUNT         2133 a         1145 c         1160 g         2360 t
ORIGIN      
        1 gaaaaaagaa gtaggtggaa tctgaaataa aaatccatca aactcttctc tcaaaactcc
       61 ttcgcccttc catgttttgt acggtcctct tagtgtaaac gtgtctgagg tattcatcac
      121 atgaactata gaatctgcat gaaccttttt tgagagctct tctttaaact catgttcttt
      181 tggaaaatat tctgtatggg taacaaaaat aagcatggtt tcatttttag aaaaggattt
      241 tcttagtaca agatgcttaa gtacaccact tttcttttcc ggatcataca cagataagtt
      301 catgtcagag attattgatt taacagtatt ccgtatttta tcaaagggtg caggcgatat
      361 tatacagttg tttatatcta ttatattatg cgtgcttgcc tctttaagac ctaaaacagt
      421 ttttttacca taagaaaatc cgaattccat ctttgatctg tactgtgttg aatcatcact
      481 ttcaattatc tgtgaaacat tgacctcaat gcttccgata cgtctgaact gttcttcaac
      541 aatcgaatgc ttagcctgca gctgctgttc gtacttcagc ccaagccact gacatccgcc
      601 acatttcgga aaatgtttgc acggagaagc cgtacggtat ggagaaggtt ttattatctg
      661 ttcaacagat gcaaagatag tgttcttttt tttgaacttc tcttttaccc ttacgatatc
      721 ctgaggataa acattctcaa ccatatagat ttcattatta taccttgcca gaccatagcc
      781 gccgtatata agcttttcaa ttacaacatc aactgaaccg ttgttttcca ttattgtcac
      841 tccattcttt ttttgtcatg taaattatat cacacttgtt caaaaatgct taaaaaagta
      901 gcattatgat ataattgttc tgtaaagggg aatgattaaa atttgttttt aaaggggggt
      961 aagtctgtga acaaaaaaga aaaaagctgg attttgtatg attgggccaa ctcagcttat
     1021 tcgatggctg ttacttcaac aattcttccg atatttttta aagatgtagc gtccaaggga
     1081 cttagcgaca ccgattcttc tgccttctgg gcatattcca actctattga aaccgttctt
     1141 ctagttctta tgcttcctat tcttggaact attgcagatt ataagaacat gaagaaaaga
     1201 ttttttattt tcttcctcct tatgggaata ttctttaccg gactgctttc tactgtgagc
     1261 gaaggtcaat ggtttttctg tctgtttatc tatgttctta ctgctatcgg attttccggt
     1321 tcaaatcttt tttatgattc tatgcttgtt gacgttacaa cagaagatag aatggattgg
     1381 gtatctacaa acggttttgc atacggctat attggaagta cgataccttt tattataagc
     1441 attgccctga taatgaaacc tcagattctt ggttttgatt cggctattat tccaactaag
     1501 ctttctttta tcataacagc actctggtgg ttcatatttt caatacctct tttgaaaaac
     1561 gtacaacaag tcaactatct tgaaccatgc acaagacctg ttaaagaaag ctttttaaga
     1621 ctcataaaga ctttcaaaga gatcaaacag tataaaaata tttttttgtt tctccttgct
     1681 tttttctttt atattgatgg tgttcatacc ataatatcta tggcaacaat atttggaaag
     1741 gatatcaatc tttctaatac aacactaatg gtagttcttc ttgtaattca gtttgttgcc
     1801 tttccttttg caattatcta tgggaagctt gcccaaaagt tctctgcaaa aataatgctt
     1861 tttgtcggga ttatagtata cacttttgta acgatctatg catatttcat aaaatctgaa
     1921 ctagaatttt ggatacttgg tcttcttgtt gccacttcac agggaggcat acaagcttta
     1981 agcagatcgc ttttcggaaa gattattccc aaagaaaaat ctgccgaatt ctttggattt
     2041 tacaatattt ttggaaaatt tgccgcaata tttggcccac tgctttatgg aatggcttct
     2101 cagataactg gaaatccaag gtttggagtc ttaagcctta taatcctttt tataatcggc
     2161 ggagttctta tgatttttgt gaaagctcaa agtccttcaa aaagttgaaa attgtgaggt
     2221 gataagtatt tcttgtttaa aaataaaagg cggatcatat tatatcaatt ctcctactaa
     2281 cataggactt tatgttttca gaaataaaaa ctgcctagtt attgatacac cacttaactc
     2341 ctctgcagcg aaaaagatcg atgaagaaat ttcttcatgt gcctttcatc caaagtatgt
     2401 tttcaatact catcatcatc ttgatcattg cggttcaaac agctattttc ggaataaata
     2461 ccccggatgt gaattttttt cctcaaaggg cgaaaaggta tttatagaga acaattttct
     2521 atactcaagc atactttggg gcgggattcc atgcagccat atagaatgga gtacaaaaga
     2581 agaaaaaatt gatgcgatcc ttgaagaagg tattaataag attaatgatg agaaatttga
     2641 aataatcgat ctgggtggcc attccaaagc tctgacggga atagttacac cagaaaaggt
     2701 tttttatatt tccgacagta ttttcagtga agaaaagctt caaaaatata aaatacctta
     2761 tatttatgat ataaagaagt ttttgatatc tgttaacaag atactatctt cggatgcgga
     2821 gtttttcctt cccggacatt cttgcaaagt atatgataaa caggagattg aacaactggc
     2881 atctaagaat gtatcagcgg tcgaattttt cagagatcag atctatacat ttttgaaaaa
     2941 tgcttttacc aaagaagaaa tagttgaaaa tataattata ttaaacgagc tttccgttga
     3001 ctttaaacag tatatgctta taagcacaac agttgccgga tatcttgaga tgttcatctc
     3061 cgaaaaattg atctcttatt ctgttcagga cggaaaacta tacttttaca gagagtagat
     3121 tataaaacga ttcctgcggc ccttatcggc gaaccatcgc cgttcttgat ttttaaaggt
     3181 aagcatgcaa acaggaactc tttaccgata agcttttctg tattgttgag gttttctatt
     3241 atacaaatgt gctttgaaag tattatttta tggtttataa gttcatgatt caccggatca
     3301 agagatattg tatcaagacc tatgccctta agattgaact gagaaagata ctgggcagcc
     3361 tgcttatcaa gtaccgggaa gttgccgaag tattcgggaa aattccaaaa tctcgcccaa
     3421 cctgtatgaa gaataacaaa atctgaggca ttaatagttt tttctgattc aagcaggtat
     3481 tttttagtta taactgcatt ttcttttaaa aaagaaaaat ccagaacaca gccttttccg
     3541 aaaaaactag atatatcttt tttgtctgta ctttcgcccg attgaaaaag atgctccgga
     3601 cagtctacat gtgttccggt gtgtgtgaac attgtgacta aggtttcttt aaaaccgtct
     3661 ttttctaaag tacaggcagt agaaagtctt ggaccatcag tgccgggata tactggcatg
     3721 gattcttcta taacatgtgt aagatcgtat actgtcatct ttcctccaag agttaagaga
     3781 atatatcgta tattcttccg atttcgcgtt cgtatttaat gaaaagttca aggattttag
     3841 gattgaaatc tgtaggtttg gttctgctgt ctccattttt aagaatttct acagcatgta
     3901 tatgagacat ggcatcttta taaggtcttt tagatctcag ggcatcgtat acatctgtaa
     3961 tggaaacaag ttgggcttca ataggtatac tttctcctga taatccagaa ttataaccac
     4021 tgccgtcata tttttcgtgg tgataaagtg ctatgttgag tgctactgtg aaataatcat
     4081 catcctgtaa aatatttttc gcatataatg tatgattctt cataacttca tactcctgtt
     4141 cagaaagctt tgactttttc atcaggagct cttttggaat aaatatcttt cctatatcat
     4201 gtagaggacc gaatttttct atctgtcgaa cggtttcttt aggtagtccg tatttaagcg
     4261 caagaaaacc tgctatctta ccaactctgt taatgtgaaa gcctgtatct tcatcatagt
     4321 tctcggcaat aagagcaagt ttatgagcga aattagtata tgcattatta attctatctg
     4381 cataaatttt tatttttaaa agcagttctg ttattttttg aaaagaagta attattttaa
     4441 tatcttcggg cgaaaaaaat atccggttaa aaaagaaaaa ataaaactgc ccgaaggatt
     4501 ttcctccgtt ttttatgggg caggcaatta tgtcagaaac attggaagta tggtctttta
     4561 aaagtttttt catatagcca tactttgaag aggctaaaat ttcatcagga gtaatttttg
     4621 taactgaatc aaagatgtct atctccttta cagtaaaatt gataaaatca atgttcaaag
     4681 ctttaggacc gacagttgat atatctttta tgttcaaatc ttcatcaaaa agaatagata
     4741 tactatagtc attcttaagc atcgaagatg tgtttatcat aagacgcctt ataatctgag
     4801 agtcagaaag aaggagtata tccgaaaact ctccagtgac atttagaaag cccataaatc
     4861 tttctttact catggtttca agccgtaata gttcattaag tcttatgttg agcttttcca
     4921 gttcttgatt tttttcatct aaaatatttt tgttttttga aatcgtttca taaagagtcc
     4981 gtaattccat ttctttcgac tgtaaacttc tgtaagagct cgccagttcc tgatttttgt
     5041 cgttgagcag atcattcttc tggtttattt cctccatctt ttgaataagt gctttggaca
     5101 ttttatcaag ttctttagat ataagcgcta tctcgtcatt cctcccgctt tcaaaactaa
     5161 tatcaaaatc tccacggcca aatgcacgaa ctttctctgc gagctcaatt atcggagagg
     5221 ttattcgatt gcttagtata aaaataaaag tgatagagaa gataattgaa acaagaagca
     5281 ccataagaac aaaattaagt attttgtttg actggtaaaa aagtttatcg gtattcattg
     5341 tcataaaaag cgtaatcgaa gaagcatact ttgtaatttt tatagggact attatattaa
     5401 gagtgattga agattcaaag taagagtaat caaagaagga attcaatttt cctgaatgca
     5461 tagcagccag tattttcttt tcatctgtgt ttctaaaaga taaaaaggat tcaaaatttt
     5521 ttccatgaaa agtatcgtct ggatgaacca tgaactttcc ggttgaagaa agaataaaaa
     5581 ctttggaatc atttactgag tttaatccag aaacaaaatt tttcaattgg atatttgaaa
     5641 tatctactcc tgcgacgcct ataaaagtat tattataaaa gattggaaca gagtagctta
     5701 tgagaagaac tgtttcattt tttatagtgt attcaaaagg atctacaatt gtttcaaggc
     5761 ctgtcctttt aggaatgttg taccagtctg aaaatgaaga aggatcatcg tatccgtaac
     5821 agctttccag atgataccca tcaatactgt tgtagtatgg tacaaatctt ccatctttat
     5881 cagcaccggg agaatttatg tattcataat cccttgcatc gaaagcatct ggttcaaaaa
     5941 ctacccatgc tccaagaaat tcagagttgt tttcaacaat attcttaagt atttttatta
     6001 catcatctcg tgaaggatta ctttgagctt tagttgtttc cagtaaaaaa gacaaaaaac
     6061 gagcattgag caatgcccgt tcaaactgtt tttcaacata acttaccgta agcatggcag
     6121 cgctttctgc ttttttcacg gcttcttttt ttatatactc gttcagtgaa atatataaaa
     6181 agattgaaat gaggaaaata aaagaaacaa atacgaaaag tgtacttgta agaacaaatt
     6241 tattaaggct tttggctctt aacaaaactt tcacctcaaa tcatatacgt ataaagatta
     6301 tagcataact ttagttattt tattcaatta acgtatttat aaattgagga agagcaaaag
     6361 aagctttatg gatattaagg ttgtaataac gtgttttagt aagtatttca tgattttgaa
     6421 ttttgggttc tatacttcca ggggtcttga atgccaaagt aaatgaccag cagcccgggg
     6481 cgtactgcgg aatataagcc gtgtataagt atgcatttga gaaaacgttt tgtatacgct
     6541 tgtaagcatt tatcatccat tctttgtgga taaaaggatc ctcagtttcg gctgaaagta
     6601 ttccatcttt ctttaaggct tccaagcacg aaagataaaa atcctctttg aaaagttgac
     6661 tagcttgacc ttctgtcgga tctgtagaat caataagaat tatgtcaaag taatcatgaa
     6721 attttttgac aaatttcgca ccgtcttcaa tgactatatc taactttgaa ttatcaaatt
     6781 cgcatgatgt gaatggaa
//
LOCUS       DAOSHR010000113         6729 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_9885_length_6729_cov_5.436620, whole genome shotgun sequence.
ACCESSION   DAOSHR010000113 DAOSHR010000000
VERSION     DAOSHR010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 6729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6729
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9885_length_6729_cov_5.436620"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            265..882
                     /locus_tag="PLU28_07100"
     CDS             265..882
                     /locus_tag="PLU28_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34865.1"
                     /translation="MDREDVINRIVQEKGTDAIPILIEQLESPDPQVSDIADEALMLL
                     AYEGKDIIMNHFKERFSLKKRDDVTLLYLAQILAHIKSPEIVPYIQKMFDMFSDERAA
                     PLLIECLLKITGDKKYLDMLYAYSQDIEFEEIIIMALGDFPSKDSLDKLMKLYELSSD
                     KSIKSLILESTLKILFSDFELVSYVNQRNPEISEKLQWALKNTNF"
     gene            892..1791
                     /gene="murB"
                     /locus_tag="PLU28_07105"
     CDS             892..1791
                     /gene="murB"
                     /locus_tag="PLU28_07105"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOG34866.1"
                     /translation="MENFFEDLYINGCEIKKNEPMKNHTYFRIGGITQAMLLPKSTEA
                     MVYTCKKLLMEGKSFRIMGYGANLIVSDSELNFFVICTKYLSDITICGTGVTVQCGVP
                     TARLSYIAAEKGLSGLEFASGIPGSVGGAVFMNAGAYDGEFKSIVRSSYVFDKKNMEV
                     LSLNADQMDFGYRHSIFHQGRYVCLETTLELLQAQRDEVIWKTKELLEKRWKKQPLDL
                     PSAGSFFKRPRNDFFVGTAIENLGMKGLRVGGAEISKKHAGFIVNAANATFDDVMKLS
                     EVVKTKIKEAYNVDLETEPEIWK"
     gene            complement(1839..3086)
                     /locus_tag="PLU28_07110"
     CDS             complement(1839..3086)
                     /locus_tag="PLU28_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34867.1"
                     /translation="MKKILLSVTALIVIAVSVLGAGLSINPYFSTFKDASGNKYLVYS
                     ITPIVDLDFMKLGIGFNAYQTEIGGKFFYGYPGISPEASNSVLNALNLNFLELDFGLV
                     AGRYGLMSNQTFGLGTVMYRYNVPLAKAFDARLGEKNGLNIKAHAPFEIGSWIPFKLV
                     QSASLAYADAQIPLGLIKVSALGATNYLGSSNNKDMVASANAYFDLFGLALGVEGGYI
                     MTSNSNTSVNTTGYGVAAGAGLDLNFFEMRLFPVAYMSKNYVMGYIDGNYEKGSTNYN
                     ALIAADKERLGGIYDVAFTIPEVITANVRYSYDLAIGENFDISKVQISNEKIYGTLAA
                     VSPFEGYPFTAYGKYSRSFKGNEHIVDMIQNIFSANTEFEYGISYSVVQGLNVSYVTF
                     LDKLDGNNPVFGSRIDLGAELSF"
     gene            complement(3323..4318)
                     /gene="galT"
                     /locus_tag="PLU28_07115"
     CDS             complement(3323..4318)
                     /gene="galT"
                     /locus_tag="PLU28_07115"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HOG34868.1"
                     /translation="MVILQQMRKDPVSDRWVIIAQDRALRPGAGKKVVAQKSNDDHCP
                     FEPGMEQETPGSRLVYEKDGRWTLRVVDNKYPALTVQEPFVEKTRGVYVSSQGYGYHE
                     VVIESPDHEEKIWSMKPQKLDEIITAYIQRSIEISCDKNIKYIQIFKNYGRQAGATID
                     HPHSQIVATPTVPVSTSYELKGAERYYEDNKKCVFCDILAQEESEGERIIFQNPGFIA
                     FEPFASRFPYETWIMPRNHSDSFLCLRDDGQSVRNLSYALKGSFELLYRLLGDFPFNL
                     VLHTSPFCSETQRYYHWYFEIIPRLYNLAGFEWGTGYNINSIPPEKAAYELKSLK"
     gene            complement(4312..5763)
                     /locus_tag="PLU28_07120"
     CDS             complement(4312..5763)
                     /locus_tag="PLU28_07120"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HOG34869.1"
                     /translation="MKIAYVSFEVAPLVKVGGLADVAGALPKYIKKLGEEIYVIMPFH
                     KAIEKNCETWKIEKIKSGMKTQSHTFSGTFDVYETAMPDTDLKIYLIKNDSLFGTEEI
                     YGYKDLAYQSSFFCDASITLIKEIEPQTGVVNVNDWQTSLISVYLKTKYRDDPVLNKI
                     ATVLTIHNLGFKGEFTSQSITDAGLPSYLFNIDALEFYGSVNFLKGGIIFSDIINTVS
                     KTYAEEIQTEEYGLGMDGVLRIRSDKLFGITNGIDYEIYDPKQNRNIYKPITDYKSKM
                     ENKARLQKDLGLNINPNVPVISFVGRLYEQKGLDIINEIMDYLMLFDVQFVILGRGDP
                     NYESIFKNYEEKYKGRFSANIKFDGLLAQQIYAGSDIFLMPSRYEPCGLGQMYAMRYG
                     TVPVVRYTGGLADTVREYYPTTKLGTGFGFYDYEACRLLMSVAKALYTYSNSRDDWER
                     IFENCMSQDFSYENTARQYKELYKIAVSAKKKW"
     gene            complement(5781..6398)
                     /locus_tag="PLU28_07125"
     CDS             complement(5781..6398)
                     /locus_tag="PLU28_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34870.1"
                     /translation="MYIKNYINTNFQKAMLTEKVSALVERFSSYVDVMIVLREDETLY
                     NCISVAEMDYIKSFDKELKLMDILDPVESFLFENDMVEDALNLIQQNRITVLPVVDYD
                     LYPVGTVGIFEIIKAFKSVSGMDEPGTKITVSLNEKPGQLRTLLDIFTSKDINILSLL
                     TSEVNNGKRSVTLKVGMKDVNAVSDLLEANSIEYDGIFEEDGGNY"
     gene            complement(6498..6671)
                     /locus_tag="PLU28_07130"
     CDS             complement(6498..6671)
                     /locus_tag="PLU28_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34871.1"
                     /translation="MNNFDKQELLKRKRVLEIEKNAIAKYMGPYEHDDFLEEEWKKIN
                     DELLELDEKLKTL"
BASE COUNT         2051 a         1399 c         1162 g         2117 t
ORIGIN      
        1 attcttaccg cctgctcaac agcataagta tccaaaggat tcaactctga ctgcagatta
       61 tctctctgca taactccggt aacaggatct atttttatat tatctgttgc cggaacctgt
      121 tttacaagaa cagccatctt catttataaa caccgccttc agtcaggaat tataaaaact
      181 tatattttaa atcatttttt aaagatagta tagtatcatt atatattaga aatgaaaaaa
      241 gagctatttt ttcggaggta tgatgtggac agagaagatg tcataaacag aatagtacag
      301 gaaaagggaa ctgatgcaat tcccattctt attgagcagc ttgaaagccc tgaccctcag
      361 gtaagcgata ttgcagatga agcgcttatg cttcttgctt atgaaggcaa ggatataatt
      421 atgaatcatt ttaaagaaag attttctctt aaaaagagag atgatgtgac tctgctttac
      481 ctggcacaaa ttcttgcgca tataaagtca cccgaaattg ttccttatat ccagaagatg
      541 tttgatatgt tttctgatga aagggcagct cccttgctca ttgaatgcct gttgaaaatc
      601 accggggata agaagtatct tgacatgctt tatgcatact cacaggatat agaatttgaa
      661 gagataatta taatggctct gggagatttt ccatctaaag actctttaga taagcttatg
      721 aagctttacg aactttccag cgataaatca attaaatctc ttattcttga aagtacttta
      781 aagatattat tctcagattt tgaattggtt tcttatgtaa atcagagaaa cccggaaata
      841 tcggaaaaat tgcaatgggc acttaaaaat actaatttct aggagacact tatggaaaat
      901 ttttttgagg atctttacat caacggctgt gaaatcaaaa aaaatgagcc tatgaaaaac
      961 catacctact tcagaatcgg cggcataact caggccatgc tccttcccaa aagtacagag
     1021 gctatggttt atacctgcaa aaaactttta atggagggaa aaagcttcag gattatggga
     1081 tacggtgcta atcttatcgt atcagacagt gaactgaact tttttgttat ctgcacaaag
     1141 tatctttcag atataaccat atgtggaacg ggcgttactg ttcagtgcgg agtcccgaca
     1201 gcaagacttt cttatatagc ggcggaaaaa ggtctttcag ggctggagtt tgccagcgga
     1261 attcccggct ctgtcggcgg agctgtcttt atgaatgccg gtgcttatga cggggagttt
     1321 aaaagtatcg taagaagttc ctatgttttt gataaaaaaa atatggaagt gctttctctt
     1381 aatgcagatc agatggattt tggctataga cacagcatct ttcaccaagg cagatatgta
     1441 tgtcttgaaa ctaccttgga acttttacaa gctcaaagag atgaagttat ctggaaaacc
     1501 aaggaacttc ttgaaaaaag gtggaaaaaa caacctcttg acctgccttc tgccggaagt
     1561 ttttttaaaa gaccaagaaa tgattttttt gtaggaacag ccattgagaa cctcggtatg
     1621 aaaggattga gggtcggagg agcagaaata tctaaaaaac atgcaggatt tatagttaat
     1681 gcagccaacg ctacttttga cgatgtgatg aaactttcgg aagtagtaaa aactaaaata
     1741 aaagaagctt ataatgtaga ccttgaaaca gaaccggaaa tatggaaata aaaaaaccgt
     1801 gccttaaagt tttactttaa ggcacggtga ttttattatc agaagcttaa ctctgcacca
     1861 agatcaattc tactgccaaa taccgggttg tttccatcaa gcttgtcaag aaatgtaaca
     1921 taacttacat taagaccctg aaccacgctg tacgaaattc cgtattcaaa ctctgtattg
     1981 gcgctgaaaa tattctgaat catatcaaca atgtgctcat ttcccttgaa agatctggaa
     2041 tacttaccgt aagctgtaaa aggatatccc tcgaatgggc ttactgctgc aagtgttccg
     2101 taaatctttt cattggaaat ctgaaccttg gatatgtcaa agttttcacc tattgccaga
     2161 tcatacgagt atcttacgtt tgctgttata acttctggaa ttgtgaacgc cacatcgtat
     2221 attccgccca gtctttcttt atctgcagcg ataagggcgt tgtagttggt gcttcccttt
     2281 tcataattgc cgtcaatata gcccataaca taatttttgc tcatgtatgc aaccgggaaa
     2341 agtctcattt caaagaagtt taaatcaagc cctgctccgg cagcaactcc ataaccggtt
     2401 gtatttacag atgtattgct gtttgaggtc attatgtatc cgccttctac tcccaaagca
     2461 agcccgaaaa gatcaaagta tgcattggct gaagcaacca tatctttatt gtttgaactt
     2521 ccaagatagt tggtggctcc aagtgctgaa accttaataa gaccaagcgg tatctgagca
     2581 tctgcatatg ctaaacttgc agactgaacc agtttgaaag gaatccatga gcctatttca
     2641 aacggagcat gtgcttttat attaagtccg ttcttttccc cgagccttgc atcaaaagcc
     2701 ttggcaagag gaacattata tctgtacatg accgtaccca aaccaaatgt ctgattcgac
     2761 ataagaccgt atcttccggc aacaagtcca aaatcaagtt caagaaaatt gaggtttaat
     2821 gcatttaaaa cactgttgct tgcctcagga gatatcccgg gatatccata gaaaaacttg
     2881 ccgcctatct cagtctgata agcattgaac ccaatcccga gtttcataaa atccagatca
     2941 actataggag ttatgctata tacgagatat ttgtttccag atgcatcttt aaacgttgaa
     3001 aaatatgggt taatcgataa tccggctccc aaaacactta ctgcgataac gataagagca
     3061 gtaacgctta aaagtatctt cttcatttta ttacctcctt gaaaggctta tacctgatat
     3121 attgatgcaa aaattaatcg cagccgcagt aaatattaca actcttttag cctgtcagtg
     3181 cgtatgcttc aactcaatta tagcacacac ggtatgttaa tcatatattt tcaaaccctt
     3241 cttaatacgt aatatctatg aaaaaataca tttcactctt tcttcttctg tttttaaatt
     3301 taaaaaccat tataaaaaga aattatttaa gcgatttaag ctcataagca gctttctcag
     3361 gaggaatgga gtttatatta tatccggtac cccactcaaa ccctgcaaga ttatagagcc
     3421 ttggaattat ttcaaagtac cagtggtaat atctttgagt ttcagaacaa aaaggtgacg
     3481 tgtgaagaac cagattaaac gggaaatctc ccagcagcct atagagaagt tcaaacgaac
     3541 cttttaaagc atatgaaaga tttcttacac tctggccatc atcccttagg caaagaaagg
     3601 aatcagaatg gttcctcggc attatccatg tttcataagg aaaccgcgat gcaaaaggtt
     3661 caaaagcaat aaatcccgga ttctgaaata ttattctctc tccttccgac tcttcctgtg
     3721 caaggatgtc gcagaagaca cattttttat tatcttcata gtacctctct gctcctttta
     3781 actcataact ggttgaaaca ggaactgtag gcgttgcaac aatctgcgaa tgaggatgat
     3841 cgatggtggc tcccgcctgt ctgccgtaat ttttaaatat ctgtatatac ttgatattct
     3901 tgtcacaact tatttctatg cttctttgaa tatatgccgt tattatttca tcaagctttt
     3961 gcggtttcat gctccatatt ttttcttcat gatccggact ttctataacc acttcatgat
     4021 atccatagcc ctgagagctt acataaactc ctcttgtttt ctcaacgaac ggttcctgga
     4081 cagtcaatgc cggatacttg ttatcaacaa ctcttaatgt ccatctgccg tctttttcat
     4141 atacaagcct gctcccgggc gtttcttgtt ccattcccgg ctcaaaggga cagtggtcat
     4201 cattactttt ctgtgcaact acttttttgc ccgctcccgg cctcaaggct ctgtcctggg
     4261 caattatcac ccatctgtca gaaacgggat ctttcctcat ctgctgcaat attaccactt
     4321 ctttttcgcc gaaacggcaa ttttgtaaag ctctttatac tgacgtgcag tattttcata
     4381 agaaaagtcc tggctcatac agttttcaaa tattctttcc caatcatcgc ggctgttgct
     4441 gtatgtataa agagctttag ccaccgacat aagaagccta catgcctcat aatcgtaaaa
     4501 cccaaaacct gttcccagct tggtcgtagg ataatactcc ctcacagtat cagccaatcc
     4561 tccggtatag cgaaccaccg gaacagtacc gtacctcatg gcatacatct gtccaagacc
     4621 gcacggctca tatcttgaag gcataagaaa tatatccgac cctgcatata tctgctgggc
     4681 taaaagacca tcaaacttta tgttggcaga aaatcgccct ttatattttt cttcataatt
     4741 tttgaaaata ctctcgtaat tgggatctcc ccgtccaagt atgacaaact gaacatcgaa
     4801 aagcatcaga tagtccatta tctcatttat aatatccaat cctttctgct catagagcct
     4861 tcccacaaaa gatatcaccg gaacgttcgg attgatgttt agtcccaaat ctttttgaag
     4921 tcttgcttta ttctccattt tacttttata gtccgttatc ggtttgtaaa tatttctgtt
     4981 ctgcttagga tcgtatatct cataatcaat cccgtttgtt atcccaaaaa gtttatctga
     5041 gcgtattctt aaaacgccat ccatgccaag cccgtactcc tcggtctgaa tctcctcggc
     5101 ataggtttta ctcacagtgt ttattatatc cgaaaatatt attccaccct tgagaaagtt
     5161 gacactgcca taaaactcca aggcatctat gttgaaaaga tacgacggaa gtccggcatc
     5221 agttatgctc tgacttgtaa actctccttt gaacccaaga ttgtgaatag tcaaaacagt
     5281 ggcgatttta ttaagcacgg gatcatcacg gtacttggtc ttaagataaa cagatataag
     5341 cgacgtctgc cagtcgttta cattgacgac tccggtctga ggttctattt cttttatgag
     5401 agttatggag gcatcgcaga aaaaagaact ctgataggca agatctttgt atccgtagat
     5461 ttcttccgtt ccgaagaggg aatcgttttt tatgagatat atcttaaggt ctgtatcagg
     5521 catagctgtt tcatatacat caaaggttcc agaaaaagta tgggactggg ttttcatgcc
     5581 agactttatt ttttctatct tccatgtttc gcagttcttt tctattgctt tatgaaaagg
     5641 cataattaca tatatttcct cacctagttt ttttatgtac tttggcaggg caccggctac
     5701 gtcagctaaa ccccctacct ttaccagagg agccacttca aaagaaacat aagcaatctt
     5761 catccgcttt ccacctcctg ctaataattt cccccgtcct cttcaaaaat gccatcatat
     5821 tcaattgagt tagcttcgag aagatcagac acagcattaa catctttcat gcctacttta
     5881 agtgtaacag acctttttcc gttatttacc tccgaagtta aaagagaaag tatatttata
     5941 tccttgctgg taaaaatatc aagaagggtt ctgagctgac cgggtttttc gtttagagaa
     6001 acggttatct tggttccggg ttcatccata cccgaaacac ttttaaaagc ctttattatt
     6061 tcaaatattc ctactgtacc taccggatag agatcgtaat caacaaccgg taaaactgtt
     6121 atcctgtttt gctgtattaa gttaagagca tcttcaacca tatcgttttc aaacaaaaag
     6181 ctctctaccg gatctaaaat atccataagc ttgagttctt tatcgaaaga ttttatataa
     6241 tccatctctg caacagaaat gcagttatac agggtttcat cctccctgag aacaatcata
     6301 acatcaacat aagaactgaa tctttcaaca agagcactta ccttctctgt aagcatcgct
     6361 ttctgaaagt tcgtattaat ataatttttt atatacatgt ctttcctccg gtcgaaacat
     6421 agacttggtt tattaatatt ataacatcaa atgattcaaa aaaaaaagga ccgcattgcg
     6481 gtcccgtcaa tagtatacta taatgtttta agcttctcat ctaattccaa aagttcatca
     6541 ttaatttttt tccattcttc ttcaagaaaa tcatcatgtt catagggtcc catatacttg
     6601 gcaatagcgt ttttttctat ctccagaact ctttttcttt taagaagttc ctgcttgtca
     6661 aagttattca tatactcacc ccctttaata ttattttacc atataattac atttcacgca
     6721 acaaaaaaa
//
LOCUS       DAOSHR010000114         6707 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_9932_length_6707_cov_6.082532, whole genome shotgun sequence.
ACCESSION   DAOSHR010000114 DAOSHR010000000
VERSION     DAOSHR010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 6707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6707
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9932_length_6707_cov_6.082532"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(91..801)
                     /locus_tag="PLU28_07135"
     CDS             complement(91..801)
                     /locus_tag="PLU28_07135"
                     /inference="COORDINATES: protein motif:HMM:NF015962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HOG34872.1"
                     /translation="MKKIISYVVPYHEKKIEHEVILLIDVLRSTTVISYLFEYGARQV
                     SMVRSVSHAMSMKGENSMVLGERKGLKVKGFDFGNSPYELKVQYEKGFNFAAHDYVIT
                     TSNGAKAFSKVYENSDVMAASFQNYLKICQYLETADDIGILCSGTKSSVSTEDLYLAG
                     KIIQNFLKKGHYTLNDDSSICLALAGLTPSQIIEKSSHALTLKKLGFINDLNLCFAEE
                     KFSAVPFSKRGSLTFKCF"
     gene            complement(817..1458)
                     /locus_tag="PLU28_07140"
     CDS             complement(817..1458)
                     /locus_tag="PLU28_07140"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOG34873.1"
                     /translation="MNIYLIRHGVTQANIDGIWQGRTIDAPLSTKGIEQARLAAQRFR
                     NIDIKAVYSSTLIRAKTTAAIISQQFGLEVKEVKELEECAIDLWSGLTIDQVKEQFGE
                     EFEMWSKDPIVSIEGVESKADLQIRAVKAVKWIINKHDEADSIIIVTHGLFLRAFFSW
                     LMQISINSRVYGLENTGISHLTFEKPDHFRILSLNETWHLDGFFSLNKDEESF"
     gene            complement(1611..2510)
                     /locus_tag="PLU28_07145"
     CDS             complement(1611..2510)
                     /locus_tag="PLU28_07145"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HOG34874.1"
                     /translation="MDMQKSKDLNGQYVSCINKVQDYIESNLASELSLNVISDVANFS
                     PFHFHRVFKVVMGESLFQFIQRTRLERAAGLLIKDGQLHISRIAADCGFLNQSVFSKA
                     FKNHFGCSASDFRKTHSSGCIKNTCYFKKQEKPIQKLDYTVRIEELQDINVVYIRYTG
                     PYKGDSALFSYLFEKLYRWVNSKGLIHFPNTKAIVMYHDNYDITQAHKLRLSACFTVK
                     SHFEPEGEFGSMIIPGGKYGVGYFELDDTQYQQAWDLMYGEWLISSRYRPDDRNSFEL
                     YPGNGSAQNRKQKVAIHIPIKDF"
     gene            2599..2940
                     /locus_tag="PLU28_07150"
     CDS             2599..2940
                     /locus_tag="PLU28_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34875.1"
                     /translation="MKKVSEQLGIQYYTVFCWRRNRKAEKELLQPILGNNQEIKSENT
                     KLKKASSILNDVLFFYKCFDNDNYRIKRIHKALIQKEINVSHRTVYRTMKEKDFIYTR
                     RTSHTITKTTT"
     gene            3157..3798
                     /locus_tag="PLU28_07155"
     CDS             3157..3798
                     /locus_tag="PLU28_07155"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HOG34876.1"
                     /translation="MAVKKVAVMIYPNFSMQEISCLTDALTVWYGIKIDVFASSKEIM
                     LSEDGFQIIANKVFEDFIVEDYSCLIMPGIIDPLPALFDEKNINFLSCLKGKDIIIAA
                     ISSAPMLLAKAGILDNISYTSGIWEEINQYLDFTPSVNNLHKPLVHDKNIITAIGFAF
                     REFAAETLQALGLECEKNIFGSIQREYSKEELTFSMGEENFKEFLKEYSEYKK"
     gene            complement(3840..4274)
                     /locus_tag="PLU28_07160"
     CDS             complement(3840..4274)
                     /locus_tag="PLU28_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34877.1"
                     /translation="MEVYRRKLKRRIWLLSVLVLFSSALGVYDVFFQTDISKDGIFCF
                     QCGLNTAIGIIAAVQIIRCRGALSNDEKLKIQFNKENDERLIAIRAKAGMPMLMVTSI
                     TIIVVAIIAGYFNTVIFITLVSAALFQLIIGLLIKLLYMKKM"
     gene            complement(4287..4490)
                     /locus_tag="PLU28_07165"
     CDS             complement(4287..4490)
                     /locus_tag="PLU28_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003097697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOG34878.1"
                     /translation="MNTKIPDLRKEKKISQEELALAVGVTRQTITSIEVGKYTASLPL
                     AYKISRYFGLKIEEVFDFSEYND"
     gene            complement(4611..6311)
                     /locus_tag="PLU28_07170"
     CDS             complement(4611..6311)
                     /locus_tag="PLU28_07170"
                     /inference="COORDINATES: protein motif:HMM:NF019874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOG34879.1"
                     /translation="MKLSIAQKFIVPAILLVLALGGIILFNNYRETYSTIYAGKLKSL
                     TDLVDSAYGILEHYDNRVKKGEITLVQAQEEVQKVIPQMRFDNGNYFFGYDMQGMNTI
                     PFGTVKVGDDKINLKDSVGREIVRDMIVLLKRDGKGEYLYLWDRSEKEKQIPKLSYVK
                     LFSPWNWFIGTGIYIDDVRAETETEFFKQLILFGSIIIVIFLILFFIGKNIKKRIQKM
                     EKTVEVFGNGNLSVNFDEAGHDEVSKISKSLNIMASNLKNILKDITESSAQIGQSSQS
                     LASIAEQTNAGSEELSGQSSKIVSNTENASANVEEMTSGIEEVASSAQLLSKSAQELS
                     QAAGKTLNAAKTGVSSIKDISFTIDDAMKKSKITLSNTQQLNERSENVGKIVDTINSI
                     TEQTNLLALNAAIEAARAGEAGKGFAVVAEEIRKLAEESSKATSQIADILGEIKSKSV
                     EVNKATQENSGAVEKIDLEMKKMSSEFEKIEEMVTTMNAGIESITSTTEEQSASAQEM
                     SAAMVKVASLIKEINEQIKSMSSAIVEQSEGAQHVSASAQELSALAENLNANTNKFKV
                     "
BASE COUNT         2080 a         1323 c         1044 g         2260 t
ORIGIN      
        1 ctgatccgaa aaaactattt cagatagccg attccagaat gtacgaagaa aaaagaaaac
       61 tttatctgag aataaaaaat tcttagacag ttaaaaacat ttgaaggtca aacttcccct
      121 tttggaaaaa ggaacggcag agaatttttc ttccgcaaag cagaggttca gatcatttat
      181 aaaccctaac ttcttgagtg taagggcatg cgatgatttt tctattatct gtgaaggtgt
      241 aagccccgct agagcaagac atatgcttga atcatcattc agggtgtaat gccccttttt
      301 gagaaagttt tgaatgatct tacccgcaag ataaagatcc tcagtagata cactgctttt
      361 agtgccggag cagagtatcc ctatatcatc ggcagtttcc aggtactggc atatcttaag
      421 ataattttga aaagaagcag ccataacatc ggagttttca taaacctttg aaaatgcctt
      481 tgcaccgttg gaagtagtga tcacataatc atgggctgca aaattaaacc ccttttcata
      541 ctgtacctta agctcatatg gagagtttcc gaaatcaaaa cctttgactt taagaccttt
      601 tctttctcca agcaccatgg aattttcccc tttcatactc attgcatgtg acacactgcg
      661 caccatactt acctgccttg caccgtattc aaaaagatac gatattacgg ttgtcgatct
      721 aagcacgtct atgagcaaaa taacctcatg ctctattttt ttttcatgat aaggaacaac
      781 atatgaaata atttttttca tacttcacct tctttattaa aaggactctt catccttatt
      841 aagtgaaaag aacccgtcca gatgccatgt ctcattcaaa gaaagtattc tgaaatgatc
      901 cggtttttca aaggtcaaat gacttatacc ggtattttca agtccatata ctcttgaatt
      961 tatggatatc tgcatgagcc agctaaaaaa tgcccttaag aaaagaccat gggttactat
     1021 tatgatacta tccgcttcat catgcttatt tattatccat ttaaccgctt ttaccgctct
     1081 gatctggaga tccgcttttg attcaacacc ttcgatagat acgataggat ctttggacca
     1141 catttcaaat tcctcaccga actgctcctt tacctgatca attgtaagac cgctccaaag
     1201 atcaatggca cactcctcaa gctcttttac ctcttttacc tcaagcccga actgctgaga
     1261 tattatagca gccgtcgtct ttgccctgat taaggtcgaa gaatatacag cctttatgtc
     1321 tatatttctg aatctctgag ccgcaagccg agcctgctca atacccttag ttgaaagagg
     1381 tgcatctatt gtccttccct gccagattcc gtcgatgttt gcctgtgtta ctccatgcct
     1441 tataagatat atattcatat aatgctcctg ttaactccct gctccaagcg ggattattaa
     1501 ataaaatact ttccagcaga tctattatac tatacataaa tgatatacct atacccttta
     1561 tcttacaaaa aaagaaaatc ccgtgcataa aacacaggat agttttaata ttaaaaatct
     1621 tttataggaa tatgtatcgc aaccttctgt tttctgtttt gtgccgaacc atttcctgga
     1681 taaagctcaa aagagttccg gtcatcaggc ctgtatctgc tgctgataag ccattctcca
     1741 tacattagat cccacgcctg ttgatactgt gtatcatcaa gctcaaagta ccctactcca
     1801 tactttcccc ctggaataat catacttccg aactcaccct cgggctcaaa atggcttttt
     1861 acagtaaagc aggcacttaa cctgagcttg tgtgcctggg ttatatcata gttatcatga
     1921 tacatcacaa tagccttagt attaggaaaa tgaatcagcc ctttcgaatt tacccatctg
     1981 taaagcttct caaaaagata cgaaaacagt gccgaatcac ctttataagg acctgtatat
     2041 cttatataaa caacatttat atcttgcaac tcttctatcc tgacagtata atcaagcttc
     2101 tgaataggtt tttcctgttt tttaaaataa catgtatttt taatgcaccc tgacgaatga
     2161 gttttcctga aatcagaagc gctacagcca aaatgatttt taaaggcctt tgaaaaaact
     2221 gactgattta aaaaaccaca atctgcagct attcgagata tgtgcaactg cccatccttg
     2281 atcaaaagac ctgcagccct ttcaagccga gtcctctgga taaactgaaa caaagattca
     2341 cccatcacta ccttgaaaac tctgtgaaaa tgaaaaggag aaaagttagc cacatcagag
     2401 ataacgttaa gcgaaagctc tgaagctaaa ttagattcaa tataatcctg aaccttgttt
     2461 atacacgaaa catactgacc atttaaatcc tttgattttt gcatatccat tatttttacc
     2521 acccggtgtt taatactaaa attatatcaa atctattcct ctttttgctc tggatggaaa
     2581 ctatctgatg aaatcggaat gaaaaaagtc tctgaacagc taggtatcca gtattataca
     2641 gttttctgct ggagaagaaa ccgtaaagct gaaaaagaat tattacaacc tatactaggc
     2701 aataatcaag aaattaaaag tgaaaacaca aaattgaaga aagctagtag tattttaaac
     2761 gatgttcttt ttttttacaa atgttttgat aacgataatt acagaataaa aagaattcat
     2821 aaagctctta tacaaaaaga aataaatgta agccaccgta cagtatacag aactatgaaa
     2881 gaaaaagact ttatttatac gcgtagaact tctcacacaa taacaaagac aacaacataa
     2941 atcaaaacta tgctattttt tattttgagt tttttgtgac aattacaagc aaaaactgga
     3001 aaataagtta taatagcatt ataaataaag cagattagat actttgtcaa aaaaatttgt
     3061 tcctatgttc tgtatgtaat ataatgtcat ttgctttttt cagagtgttg ctttctataa
     3121 atacaaatat ttttttaaat gctgaggagg cgttatatgg cggtaaaaaa agttgctgtt
     3181 atgatttatc ctaatttcag catgcaggaa ataagctgcc ttacagatgc tttaactgtt
     3241 tggtatggta ttaagattga tgtgttcgca tcttcaaaag aaattatgct ttcggaagat
     3301 ggatttcaga tcattgcaaa taaagtattc gaagatttta ttgttgaaga ttacagctgt
     3361 cttatcatgc cgggtattat agatccttta cctgcgttgt tcgatgaaaa aaatattaac
     3421 tttttatcgt gtcttaaagg caaagatatt attatagcag ctatatcctc tgccccaatg
     3481 cttcttgcaa aagctggcat acttgataat atttcctata cctccggcat atgggaagaa
     3541 ataaaccagt atcttgattt cactccatct gttaacaatc ttcacaaacc cctggtacac
     3601 gataaaaata taattactgc cataggtttt gcttttcgtg agtttgcggc agagacttta
     3661 caggctttgg ggcttgaatg tgagaagaat atttttggta gtattcaacg tgagtattcc
     3721 aaagaagaac ttacattcag catgggagaa gaaaatttca aagaattttt gaaagagtac
     3781 tcagaatata aaaaataatt ccggaatttc cggaattatt tttttaagtt ttataaatgc
     3841 tacatctttt tcatatagag taattttata agcaacccga ttatcaattg gaacaaggct
     3901 gcacttacaa gagtaataaa aataacggta ttgaagtacc ctgctattat tgccacaacg
     3961 ataatagtta tagaagtaac catgagcatt ggcattcctg ctttagctct tatcgctatg
     4021 agtctttcat cattttcctt attgaactga atctttagct tttcatcatt acttaaagct
     4081 cccctgcatc gtataatctg tactgctgca ataataccta ttgctgtgtt taatccgcac
     4141 tgaaaacaaa agattccgtc tttgctgata tctgtctgaa aaaaaacatc ataaactcca
     4201 agagcagatg agaaaagaac tagtactgaa agtaaccaaa ttcttctttt tagttttctt
     4261 ctatatacct ccatatttcc accttctcaa tcgttgtatt ctgaaaaatc aaatacttct
     4321 tctattttta atccgaaata acgagatatc ttatacgcta acggcagtga tgcggtgtac
     4381 tttcctactt caatagaggt aattgtctgg cgtgtaactc ctacggccag cgcaagctcc
     4441 tcctgcgaaa tttttttctc ctttcttaag tcagggattt ttgtattcat ctactgctcc
     4501 tttttaaaga aagtctattt tttctagtat agcatgcttt gcattttttg tcaagtaaac
     4561 tttgcaaaac aaaactcttc cacatgggaa gagttttttt gggttatttt ttatactttg
     4621 aacttatttg tatttgcgtt aagattttcg gcaagagcag acaactcttg tgcgctggca
     4681 cttacatgct gcgctccctc tgactgttct actattgcgc tggacatact ttttatctgc
     4741 tcatttatct cttttatgag tgatgcaact tttaccattg cggcactcat ctcctgggcg
     4801 gaagcacttt gttcttcggt cgttgaagta atactttcta ttccggcatt catcgtagta
     4861 accatttctt caattttttc aaactctgaa gacatctttt tcatttctaa gtctatcttt
     4921 tcaacagctc cagaattttc ctgagttgcc ttgttcactt cgacagattt gcttttaatc
     4981 tcacctagaa tatcagctat ctgtgaagtg gctttggagc tttcttcggc cagtttacgg
     5041 atttcctcag ccacaaccgc aaaacctttt ccggcttctc ccgctcttgc agcttctatc
     5101 gcagcattaa gagctagcaa attggtctgc tcagttatcg aattgattgt atctactatt
     5161 tttcctacat tctcagaccg ttcattaagc tgctgtgtat tggataatgt gattttagat
     5221 tttttcatag catcgtctat ggtaaaagat atatctttta ttgaactaac accggtttta
     5281 gccgcattaa gcgtttttcc ggccgcttgg cttaactcct gtgcagattt tgaaagaagc
     5341 tgtgcactag atgcaacttc ttcaatccct gaggtcatct cttcaacatt agcacttgca
     5401 ttctctgtgt ttgaaacaat ttttgaactt tgccctgaaa gttcttcgct tccggcattt
     5461 gtctgctcag caatagatgc aagactttgg cttgactgac ctatctgagc tgaagactct
     5521 gttatgtctt taagaatgtt cttgagattt gatgccataa tattcagact ttttgatatc
     5581 ttcgatactt catcatgccc tgcttcatca aaattcacag aaagatttcc gtttccaaat
     5641 acttcgaccg ttttttccat tttttgaatt ctttttttta tgttttttcc tataaaaaat
     5701 aaaatcaaaa atattactat tattattgaa ccaaacaaga ttaactgttt aaaaaattct
     5761 gtttcggttt cagctcttac atcatctata tatattccag tacctatgaa ccagttccaa
     5821 ggagagaaaa gctttacgta tgagagttta ggaatctgct tttctttttc agatctatcc
     5881 cagagataaa gatattctcc tttaccatct cgctttagaa gtacaatcat atctctcact
     5941 atctctctgc caacagagtc tttgagattt attttatcgt ctccaacttt taccgtacca
     6001 aaaggaatag tattcattcc ttgcatatca tagccaaaaa aataattacc gttatcaaac
     6061 cgcatttgag gaatcacttt ctgcacttct tcctgagctt gaactaaagt tatttcacct
     6121 ttttttacac ggttatcata atgctcaagg ataccatatg cactgtctac aagatctgta
     6181 agagatttaa gcttaccggc atatatcgta ctgtaagttt ccctatagtt gttaaacagt
     6241 ataatgccgc ctagtgcaag aacaagtaaa atcgccggaa ctataaattt ttgcgctata
     6301 gatagtttca taaaaacacc tccgtaagaa atattaagga gaaaatacat ctgctactat
     6361 catatcacat ttgttaataa aaaagaaagg cttttatcaa aaaaacaaaa gcatataaat
     6421 gaaaaaaata atttcactta tatgctttct attacataaa gtagtaaatt gatatgattc
     6481 gtatcttata aatttagtcc acctagtaaa caacggacta aactcaaaac cttataggta
     6541 tgctaggaag ttgcgccggt gtttattttt aacaatgtac aatatagttt caaaacctta
     6601 taggtatgct aggaagtttt tcatactttg tctattattt gcagcttttt atgtttcaaa
     6661 accttatagg tatgctagga agtttttcat actttgtcta ttatttg
//
LOCUS       DAOSHR010000115         6669 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_10004_length_6669_cov_5.296946, whole genome shotgun sequence.
ACCESSION   DAOSHR010000115 DAOSHR010000000
VERSION     DAOSHR010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 6669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6669
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10004_length_6669_cov_5.296946"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..1326)
                     /locus_tag="PLU28_07175"
     CDS             complement(76..1326)
                     /locus_tag="PLU28_07175"
                     /inference="COORDINATES: protein motif:HMM:NF024941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4127 family protein"
                     /protein_id="HOG34880.1"
                     /translation="MKNRIVFLPVDERFCTRDYFLLLAKAAGLNVVTPDKRLLGMKKT
                     APDIQRLHEWIINNVRGDDILIASVDMIVHGGLIPSRMSLESDQTLNKRLEILDYLKS
                     IGTKIYAGVCVTRAPFYNSAEEEPDYWQYYGQDIYNISRELVKFYENQASASELAQSI
                     AKVPSWISEDFFIRRNRNFRFVSRVIDYVKKGTIDFLNLTLDDNTQGSLSLWESEKHQ
                     KKAGDLGILSKVSIHAGADESTLTLLAKVLCDEFKVSPKFKVVYTEPEYKNFIPAYEG
                     RPLEQGIKSHILSAGGLLCDDGEDITFYAHNPTNRKESNEQEYIESDRYTATVDVLKK
                     SESIVGIACIKYTNGSDNSMAKAVLNARLNWNIFNFAGWNTAGNTVGTVCAYSIIQYL
                     GKKGLLKIDSYEMLKLQFVFFLEH"
     gene            complement(1345..2376)
                     /locus_tag="PLU28_07180"
     CDS             complement(1345..2376)
                     /locus_tag="PLU28_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HOG34881.1"
                     /translation="MKKILVVLMLLTAVFSFASDFIISTGKPYITTNPVNESYPDNGG
                     IMMTDGNFRFMWADMVAVQKPTETSNPEFTIDLGQIYEEISYVAVKFMLSKPSAVKAP
                     DSFIVSSSEDDELYIVEGMGTDILEPVEDNKILTMYFVPDEPITAQFINVEIRRSNNS
                     GWVMVNEIQVGNGPLPQEILSALPVLDTSEKTYENISLGKPYLVNPASSEAYPDGGVK
                     ITDGKFAYSWADMIGFNAPVSNPTVTIDLGEVKNIDMVTGHFMRSNASAVPIPMCMII
                     SVSDDDEVYYEVGIAATWDPPAENEKINQIVWKNRGEKITGQYVMIEIIPRGKAWTML
                     AELSVFSAQ"
     gene            complement(2401..3234)
                     /locus_tag="PLU28_07185"
     CDS             complement(2401..3234)
                     /locus_tag="PLU28_07185"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOG34882.1"
                     /translation="MTKVKTRRLVLTYMFAVFMSILFLFPFLWMTTTSFKTADQVYDN
                     PMQIIPNPFDFSPYQRLFTENNFGQYLWNTVYVTVLTLIGVILSSSVVAFGFSYFRFP
                     KKELLFKILLGTMMLPAQVTMIPQYILFDKLGWIGSFKPLIIPTFFGGTWVIFLLRQF
                     FDTIPREYSEAAKIDGANEWTIFTKIYLPLAKAPISTSVLFVFMWTWTDFMNPLIYLT
                     DDKMYTLSIALQRISSGARSTDWPLMMAGSLVMALPIVILFFSAQKTFINGASMSGLK
                     G"
     gene            complement(3236..4144)
                     /locus_tag="PLU28_07190"
     CDS             complement(3236..4144)
                     /locus_tag="PLU28_07190"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOG34883.1"
                     /translation="MKRDISFSARSRRREVLMGLLFILPWILGLLIFTLYPVVASFIL
                     SFTRTGEGSAIKWVGLDNYITAFREPDFFRAILNTLILSLIGIPVAQIMSLVAALLLN
                     VKMKFQGVFRTIFFLPSVVPAVATGLLWKWILNPRLGPINTFLDSIGLAGPGWLADPA
                     WVKPSLIIMSFWGIGGTMVIYLAGLQNIPTEYYEAAKLDGAGSWACLKYITMPMLRPV
                     TLFNTIMAIITSFQSFSNIIIMLDMDGGMKKAGLVYGLYIYTNAFRDFKMGYSSALAW
                     ILLAFTFVSVMLTYKFFNRSGDQEVS"
     gene            complement(4141..5394)
                     /locus_tag="PLU28_07195"
     CDS             complement(4141..5394)
                     /locus_tag="PLU28_07195"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOG34884.1"
                     /translation="MRKMILVLLIMSVAVISFCAKEVVFWNAWGGDEGKALDSIIDRY
                     NKSQSKVYVRSVYVPIGMGEKIMTTLSGSTTPDVITVWDWMVVPLGYRGQIVPMNDRL
                     AKAGITSSDYIKGVWEYGTYKGKKYGLPTTLNVSAFMWNKNAFKEVGLNPDVPPKTIK
                     ELDEFTDKLNKYDKKGALKRLGFDPVISHIYCYVFGGQLWDPATNEITANHQKNIEAY
                     EWLSSYYKKVDLQKKRIFQAGWGDLFSSLNPFLRGEIAMKEGSQWEILIAKKYGKSTF
                     DYGISNFPAPQGGRNDVCNVSGSFWVIPTASKNQDEAFEFLTWLTADKQAAEFAAALY
                     NIPPKIKSLEDNAFKTIVDDKMKIYIDMLMNGYVYTFPMLPIGQAYLTELTQALMDVQ
                     LGNKTAKEALDYVQERINQELKNYK"
     gene            complement(5394..6278)
                     /locus_tag="PLU28_07200"
     CDS             complement(5394..6278)
                     /locus_tag="PLU28_07200"
                     /inference="COORDINATES: protein motif:HMM:NF025841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 domain-containing protein"
                     /protein_id="HOG34885.1"
                     /translation="MTGAFFQLENGYASYGEDFWEAELKSMKEIGMDSIIIQYTKTQK
                     DCFFSSTYSNCKIVNTIIRIAEKYSLKIFLGLGLFNFNYDSGFDLDTEIQININLFRQ
                     LNGIFKESPSLAGWYIPHELDDRNFNSLYAKKKVYKFFSTVSDFIHTNSSLPVMISPY
                     FGINPDSRSYSAWWNEFFKYSVIDILAMQDGVGTKKVDYISASMVFKDLEDTMKKNNV
                     EFWADIEVFEAVHGYPIDLQPWQAKYADFERVKKQLEYISPYVSKSVIFDYPHYFSPR
                     LKNGLYENYQNLLKGGTL"
     gene            complement(6300..>6669)
                     /locus_tag="PLU28_07205"
     CDS             complement(6300..>6669)
                     /locus_tag="PLU28_07205"
                     /inference="COORDINATES: protein motif:HMM:NF028444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5109 domain-containing protein"
                     /protein_id="HOG34886.1"
                     /translation="LFWEEIFKQAKIDIFAMQDGVGTKRTTAQESAQVYKALKPVMDK
                     YGVKFWANLEVFEQTHGWPVDDKPWQSTTAKIERVKTQMELMSPYVEKCVIFDYPSYM
                     SPRLENPLYDNYSKYYQEIK"
BASE COUNT         1972 a         1402 c         1205 g         2090 t
ORIGIN      
        1 cacagaccat ggcggaacac ctttttcgtc gtttgccttc ttaataagat ctattcggac
       61 attgctctga aaggctcaat gttccagaaa aaagacaaat tgaagcttaa gcatttcata
      121 actgtctatc ttcaaaagac ctttctttcc gagatactgt attatgctgt atgcgcatac
      181 agttccaaca gtgttcccgg ctgtgttcca gcccgcaaag ttaaagatgt tccagttaag
      241 acgggcattg agaacagctt tggccatcga attgtcagaa ccatttgtgt actttataca
      301 tgcaattcct acgatacttt cagatttttt aaggacatca acagttgcag tatacctgtc
      361 tgattcaata tattcctgtt cattgctttc tttacggttt gtaggattgt gggcgtaaaa
      421 agttatatct tcaccgtcat cgcataacag ccctcccgca ctcaatatat gactctttat
      481 gccctgctcc agaggtcttc cctcgtaagc aggaataaag tttttatatt ctggttctgt
      541 ataaaccact ttaaatttag gggatacttt gaactcgtca caaagaactt tagcgaggag
      601 tgtaagtgtt gactcatcag ctcctgcatg tattgaaact ttggagagta tgccaagatc
      661 accggccttc ttctggtgct tttctgactc ccacaaggaa agacttccct gcgtattgtc
      721 atcaagagta aggttgagaa agtcaatagt tccttttttt acataatcga tgacacgtga
      781 tacaaatctg aaatttctgt ttcttcgtat aaaaaagtct tctgatatcc agcttgggac
      841 tttggcgata ctttgtgcga gttcgctggc ggatgcctga ttttcataaa actttaccag
      901 ttcacgcgat atgttgtata tgtcttgccc ataatactgc caataatcag gttcttcttc
      961 ggcagagtta taaaagggtg ccctggttac acagactccg gcatagattt ttgttcctat
     1021 tgatttgaga taatcaagaa tttcaagacg tttattaagt gtctgatcac tttcaagact
     1081 cattctagaa ggaataagac caccgtggac tatcatatcc acagacgcaa tcagtatatc
     1141 atcgcctctg acattgttta tgatccattc atggagtctt tgtatatcag gagcagtttt
     1201 tttcattccg agcagtcgtt tatcgggagt aaccacattt aaacctgctg cttttgcaag
     1261 cagcaggaaa taatctctag tacagaatct ctcatctacc ggtaaaaaaa caattctgtt
     1321 tttcattttc aaactcctgt cagattattg tgcagagaaa acagaaagct ctgcaagcat
     1381 ggtccatgct tttcctcttg ggattatttc aatcataacg tactgaccag ttattttttc
     1441 tcctctgttt ttccagacta tctgattaat cttttcattt tctgccggag gatcccatgt
     1501 agcggctata ccaacttcgt aatatacttc atcatcatcg cttacggaaa ttatcataca
     1561 cattggtatc ggaactgccg aagcatttga tctcataaaa tgccctgtta ccatatcgat
     1621 gttctttact tcaccaagat caatagtgac agtaggattt gaaacaggtg cattgaaacc
     1681 gatcatatct gcccaagagt atgcaaactt tccatcggtt atttttacgc cgccgtctgg
     1741 gtaagcttcg ctggatgccg gattaacaag atatggtttg ccaagagata tattttcgta
     1801 tgttttttca gaagtatcca aaacaggaag tgctgagagt atctcctgtg gaagagggcc
     1861 atttccaacc tgaatttcat ttaccataac ccatccgctg ttattggatc ttcttatttc
     1921 aacattaata aactgtgcgg ttataggttc atcaggaaca aagtacatgg taagtatttt
     1981 attatcttca acgggttcaa gaatatcagt tcccatacct tcaactatat aaagctcatc
     2041 atcttctgat gaagatacta taaagctgtc tggagctttt acagccgatg gttttgaaag
     2101 cataaactta acagctacat agcttatttc ctcgtatatc tggcccaagt ctattgtaaa
     2161 ctcagggttc gatgtttctg taggtttctg tactgcaacc atgtctgccc acataaatct
     2221 gaagtttcca tcggtcatca ttattccgcc gttgtccgga tagctttcat ttaccggatt
     2281 tgtagttatg tacggcttgc ctgttgatat tataaaatct gaggcaaagc taaaaactgc
     2341 tgtgagaagc ataagaacaa ccaaaatctt tttcatactt acccctccta taaaatagtt
     2401 ttatcctttt aaaccactca ttgatgctcc atttatgaaa gttttttgtg ccgagaagaa
     2461 aagtattact ataggtaaag ccattacaag tgaacctgcc atcatcagcg gccaatccgt
     2521 gctcctggct cctgaagata ttctctgtaa tgcgatcgaa agagtataca tcttatcgtc
     2581 agtgaggtat atgagcggat tcatgaaatc tgtccatgtc cacatgaaaa caaaaagaac
     2641 cgaagtagag ataggcgcct ttgccaacgg taggtatatt tttgtaaaaa ttgtccattc
     2701 attcgcaccg tcaatctttg cggcttcgga gtactctctg ggtatagtgt cgaaaaattg
     2761 tcgcagaaga aatattaccc atgttccccc gaaaaaagta ggaattatga gaggcttaaa
     2821 agaacctatc catcccagct tgtcaaaaag aatatactga ggaatcatag taacctgagc
     2881 aggcagcatc atggttccga gcaaaatttt aaaaagcagc tccttttttg gaaatctgaa
     2941 gtatgagaag ccaaaagcca ccaccgatga agacagaata actccgataa gcgtaagtac
     3001 cgttacatat actgtgttcc acaaatactg accgaagtta ttttctgtga acaatctttg
     3061 ataaggagaa aaatcgaaag gattgggaat tatctgcatc ggattatcat aaacctgatc
     3121 ggccgtttta aaagatgttg ttgtcatcca tagaaaagga aaaagaaaca aaatactcat
     3181 aaataccgca aacatatatg tgagaactaa tcttctggtc tttacttttg tcatatcatg
     3241 acacctcttg atcgccggat ctgttgaaga atttatacgt aagcatcact gaaacaaagg
     3301 taaatgccag caaaatccat gcaagagcac ttgaatagcc cattttaaaa tctctgaaag
     3361 cattcgtgta aatatatagt ccataaacca agcccgcttt tttcataccg ccatccatat
     3421 caagcatgat tattatatta gagaatgact gaaatgaagt tattatagcc attatggtat
     3481 taaaaagggt aaccggcctg agcattggca tggtaatgta cttcagacag gcccatgatc
     3541 cggcaccgtc aagcttggct gcttcatagt attcggtagg tatattctga agtccggcaa
     3601 gataaataac catagttccg cctattcccc aaaaactcat tatgatcagc gacggtttaa
     3661 cccatgcagg atctgccagc caacctggtc ccgccaatcc gatggaatca aggaatgtgt
     3721 ttataggtcc aagacggggg ttgagaatcc atttccaaag caatcctgta gctactgccg
     3781 gaacaacgct gggaagaaag aatatggttc tgaatacccc ctgaaacttc atcttcacat
     3841 tcagcagcaa agcagctaca agactcatta tctgtgcaac aggaataccg ataagcgaca
     3901 gaatgagagt attcaatata gccctgaaaa aatccggctc tctgaaagct gttatgtaat
     3961 tatccagacc aacccattta attgcagagc cttcaccggt tctggtaaaa ctcaaaataa
     4021 aagatgccac tacagggtat aaagtgaata taagcagccc taagatccat ggaagtataa
     4081 aaaggagccc cataagcact tcccgccttc ttgatcttgc agaaaaagaa atatctctct
     4141 tcatttgtag tttttcaact cctggtttat tctctcctga acataatcaa gagcctcttt
     4201 ggcggtttta ttacccagct gcacatccat aagagcctgc gtaagttctg tgaggtatgc
     4261 ctgacctata ggaagcattg gaaaagtata tacatagccg ttcatcaaca tatctatata
     4321 aattttcatc ttatcgtcaa caatggtctt gaaagcatta tcttcaagag atttgatctt
     4381 tggaggaatg ttgtaaagag ctgcagcaaa ctcggctgct tgtttatctg cagtgagcca
     4441 tgtcagaaat tcaaaagctt catcttggtt ctttgaagcg gtaggaatca cccaaaaaga
     4501 accacttaca ttacatacat catttctgcc gccctgagga gcaggaaagt tggatattcc
     4561 gtaatcaaag gtacttttgc cgtatttttt tgctattagt atctcccact gtgatccttc
     4621 tttcatagca atttcacctc tcaggaaagg attgagagaa ctgaaaagat ctccccaacc
     4681 ggcctgaaaa attctctttt tctgaaggtc aactttttta tagtaagagg aaagccattc
     4741 atatgcttct atattttttt gatggttggc agtgatttca tttgttgcgg gatcccagag
     4801 ctgtccgccg aaaacataac agtatatatg acttattaca gggtcaaatc ctaatctttt
     4861 cagagcgcct tttttatcgt acttattaag cttgtcggtg aactcatcca attcttttat
     4921 ggttttaggc ggaacatcag gattgagacc tacttcttta aaagcatttt tattccacat
     4981 aaaagcactt acattcaaag ttgtaggcag accatatttt ttacctttat aggttccata
     5041 ctcccataca ccttttatgt aatcagaaga agttatccct gcttttgcca atctgtcgtt
     5101 catagggact atctgtcctc tgtatcccaa aggaacaacc atccagtccc atacggttat
     5161 tacatcaggg gttgtgcttc cggaaagagt tgtcattatt ttctcaccca taccgatagg
     5221 tacatacaca gatctgacgt atactttaga ctgggattta ttgtatctgt ctataatgct
     5281 gtcaagggct tttccctcgt ctcctcccca tgcgttccag aaaactactt cctttgcaca
     5341 gaacgaaatg actgctacag acataataag aagaacaaga atcatttttc tcattataaa
     5401 gtccctcctt taagcaagtt ttgataattt tcataaagtc cgttttttag tcgcgggctg
     5461 aagtaatgag gataatcgaa aataacactt ttgctgacat aaggagaaat gtactccaat
     5521 tgttttttaa ctctttcaaa gtctgcatat tttgcctgcc acggttgtaa atcaatagga
     5581 taaccgtgta ctgcttcgaa tacctcaata tccgcccaga actcaacgtt attttttttc
     5641 attgtatctt caagatcttt aaataccata gatgcactga tgtaatcaac ttttttcgta
     5701 ccaactccgt cttgcatggc cagaatatct atgacagaat atttgaaaaa ttcattccac
     5761 caggctgaat aagatctgga gtcagggttt attccgaaat aaggcgaaat cattaccgga
     5821 agagaactgt ttgtatgaat gaagtctgaa actgtactaa aaaacttata tactttcttt
     5881 ttggcataca aggagttaaa gtttctgtcg tccagttcat gaggtatata ccacccggca
     5941 agagaaggac tttccttgaa tattccgttc agctggcgga aaaggtttat gttgatctga
     6001 atttcagtat caagatcaaa cccgctgtca taattaaagt taaataaccc gagccccaga
     6061 aagattttta agctatattt ttctgcaatt ctgataatag tatttacaat cttgcagtta
     6121 gaataagtac tgctgaaaaa gcaatctttc tgggtcttgg tatactgtat tatgatactg
     6181 tccataccga tttccttcat gctttttaac tcagcttccc aaaaatcttc tccgtaagaa
     6241 gcataaccgt tttccaactg aaagaacgca cctgttattt ttattttgag tccctccgtt
     6301 tactttatct cctgataata ctttgaataa ttatcataga gaggattctc aagtcttgga
     6361 ctcatatagc ttggataatc aaagataaca cacttttcta cataaggaga catcagttcc
     6421 atctgggttt taactctttc tatcttagct gtggtagact gccatggctt atcgtcaacc
     6481 ggccatccat gagtctgctc aaaaacctcc agattggccc agaacttaac tccatactta
     6541 tccattacag gcttaagagc tttatatact tgtgccgatt cctgcgctgt agttcttttg
     6601 gtacctactc cgtcctgcat agcaaaaata tctatttttg cctgtttaaa gatttcttcc
     6661 cagaagagt
//
LOCUS       DAOSHR010000116         6495 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_10398_length_6495_cov_5.654037, whole genome shotgun sequence.
ACCESSION   DAOSHR010000116 DAOSHR010000000
VERSION     DAOSHR010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 6495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6495
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10398_length_6495_cov_5.654037"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="PLU28_07210"
     CDS             complement(<1..241)
                     /locus_tag="PLU28_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG34887.1"
                     /translation="MSLELKNIRKVFKSDGVENIAVEDFNLSINKGELVTFLGPSGCG
                     KTTTLRMVSGFEVPSSGKVILEGTDITNDPPNKRDI"
     gene            complement(247..1929)
                     /locus_tag="PLU28_07215"
     CDS             complement(247..1929)
                     /locus_tag="PLU28_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOG34888.1"
                     /translation="MTLIKEESKNRFKALLKNPTVLVMVILIFIALFTFIVYPLIKVL
                     QTSFTDNSGNFSLEVYKTVFKSGYIRQGFYNSILVAVLTAVIGTFLGFLYAYAINRAG
                     LPFKRFFKAVALMPMVFPPFIGALSIIMLFGFNGLITAGIFGVRNFPVYGLWGLMIAQ
                     TISFFPVAFITLDGVISTISPTLEDAAFNLKANRLQVFFKVILPLSVPGIASTMLVLF
                     IESLADFGNPLILAGSRFPILSVQAYLQITGMFDLKSGAAMAVWLLLPSLIAFILQKY
                     WIGKKKYITVSGKPTTSSIKSVSPFAKWLLFLFVLLVSLFILLIYVIIFFGAFSKIWG
                     MNNTLTLENFRYVLDVGIESIKDTLTLALTSTPISAVLGMIIAFLLVRKVFPGKKIME
                     FTSLLSFAVPGTVIGIGYILAFNNKPLLLTGTLTILVLNFVFRYIPVGIQSGMALLNQ
                     VDPAIEEAAYTLGADDRTVFGKVTLPLIIPAFFSACVYSFVRSMTAISAAIFLVSANW
                     NLMTVQILSQTDSGRLSEACAFSVILILIIFAFMGILKIFLKNKVSLINGQR"
     gene            complement(1969..2949)
                     /locus_tag="PLU28_07220"
     CDS             complement(1969..2949)
                     /locus_tag="PLU28_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOG34889.1"
                     /translation="MKKFLAFLGILLSVSALFAELIVYSSVDEANARKILNKFSEETG
                     VKVKFVFLSSGPALTRLEAEKTNPQADVWFGAPMSNHILAKEKKLTTPYNLKIENISQ
                     DFYDGEGYYHTFYMNPIGIGVNLKVMEQIKAAVPKTWQDLTKYVYTNMIQYPNPQSSG
                     TAYSFITGLVKIYGEDEAFNYLKKIAKNVQSYTQSGTGPSKTVGVGQAGIGIQFTPAF
                     FEFKDQGYPIEIVFPEDGVPYESACVSIVNGTKNMQEASKLVDWLMSKNGQQAIVDAK
                     TYFYPVRSDVNFGNLQPLSTIKLITVDEKWAADNKKRLTDKWIELVLPVK"
     gene            complement(3131..3592)
                     /locus_tag="PLU28_07225"
     CDS             complement(3131..3592)
                     /locus_tag="PLU28_07225"
                     /inference="COORDINATES: protein motif:HMM:NF024457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3919 family protein"
                     /protein_id="HOG34890.1"
                     /translation="MVCLNKKLFLAAMNFFLVLFLIFFFGLKSIYEKFDRPVFIDSVQ
                     SSSSRLEESIPLKIEFSMDGKKYKLENPEVIIDLWSRIRSLPYYTENTIPSDGEQSVT
                     GEFYFLDNNTSSFILNKDLRIDGYYYGSENNPVISYFKDILEKISEEQSLK"
     gene            complement(3576..4997)
                     /locus_tag="PLU28_07230"
     CDS             complement(3576..4997)
                     /locus_tag="PLU28_07230"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOG34891.1"
                     /translation="MRFSLRSKIIIQNAMILILLFSAIFFIITNTLYSTVINKTVESL
                     NSDLSFYQKYISGFIANKTLSQALDSFVSKSPFLCAYIGNKLGIQTEIYNNRKDLLGI
                     SYPKNLYYVYQDVYYASNGKRTYIIKNTGGTQTLFISCPIYHENEVIGCLRYVYPLVQ
                     EMELLYKNTYVILLLGVVSLLTGGVISYFYAYSITRPLVKLKDEADKIAEGTFNARIS
                     ISSNDEIQDLSSSFNVMAEKLSKYIDNLKQEKQKQKRFVDNVAHELKTPLTSIIGYSD
                     ILPKIKDKKDFDTATGYINQQGKRLLELVEEMLYMSNLGRNEFELNLSVFDIKKAVLE
                     CIQALKPRLDKFGIKTEAALENSYVLADYAKTQEVLMNILDNCIKHSDCTVIHINTES
                     SKDTYLLDIIDNGDGISKDDLEKIFEPFYTTGKNQKKSSGLGLFIAREIMKKQNGSIN
                     IISEENNYTRVTLSLRKGDGLSE"
     gene            complement(4999..5673)
                     /locus_tag="PLU28_07235"
     CDS             complement(4999..5673)
                     /locus_tag="PLU28_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOG34892.1"
                     /translation="MKILVVDDEQSILDLVRMSLILENYDVITLDSGKDFSSVIEHEN
                     PDFIILDVMLGIYDGLNLLKELRSSGVDTPVILLTAKSQINDRLMGLYCGADDYISKP
                     FDSRELILRIKAIEKRMDRNHSQKSCVLSSGFLRMDQSARKAFIDGSEVYLTKKEFDV
                     LFLLVKNNGTVFRREELLTQVWGYDSSIDTRALDALIQRIRKKLGIHGESIVTLYGIG
                     YKFEVQ"
     gene            6027..>6495
                     /locus_tag="PLU28_07240"
     CDS             6027..>6495
                     /locus_tag="PLU28_07240"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOG34893.1"
                     /translation="MKKAITVMVLVLLILAVPVKSQEILQDQNIEMVLVEKGSFLMGN
                     LFIEGDENEKPLHNVTFTYDFCIGKYEVTFDQYDIYCEETKKATPYDIGWGRGNRPVI
                     NVSWYDAIGYCNWLSEKEGLAKAYDSEGKFLDSNGNITTDPSLVEGYRLPTEAE"
BASE COUNT         2008 a         1211 c         1235 g         2041 t
ORIGIN      
        1 atatatcccg tttgttggga ggatcattgg ttatgtctgt tccttccaga atgacctttc
       61 ctgatgacgg aacttcaaat ccggatacca tccttagagt tgtggttttt ccacagcctg
      121 acggacccaa aaaagtgaca agttcacctt tgtttatgga aagattaaaa tcttcaaccg
      181 cgatattttc aactccgtcg cttttaaata cttttcttat gtttttaagt tcaagactca
      241 atgatatcac ctctgcccgt tgattaagga tactttattt ttaagaaaaa ttttcaggat
      301 acccataaaa gcaaaaatta tcagtatgag tataactgaa aatgcacatg cttcggaaag
      361 tctgccggaa tctgtctgtg aaagaatctg tacggtcatc agattccagt tggctgatac
      421 aagaaagatg gctgcactta tagccgtcat ggagcgtacg aaagagtaaa cacaggcaga
      481 gaaaaaagcc ggtattataa gcggcagggt tacttttccg aaaacagttc tgtcatctgc
      541 tcccaaagtg tatgcagcct cttcgattgc cgggtcaacc tgatttaaaa gagccattcc
      601 ggattgaata cctactggaa tatacctgaa tacaaagttc agcacaagta tggtaagagt
      661 tccggtaagc aggagaggct tattgttgaa agccaaaata tatcctatgc ctatgactgt
      721 tccgggaact gcaaaactca aaagggaggt gaactccata atctttttcc caggaaatac
      781 tttccgcaca agcaaaaagg caattatcat tcctaacacg gccgagatcg gtgtggatgt
      841 aagggcaagt gtaagagtat cttttattga ttcaatgcca acatcaagta catatctgaa
      901 attttccaaa gtaagggtat tgttcatacc ccatatcttg gagaatgctc cgaaaaaaat
      961 tattacatat atcagaagta taaataagct tactaaaagt acaaaaagaa aaagtagcca
     1021 ttttgcaaaa ggacttacgc tttttataga tgaggttgta ggttttccgg aaacagttat
     1081 gtattttttc ttaccgatcc agtatttttg cagtataaaa gctataaggc ttgggagcaa
     1141 aagccatact gccattgccg caccgctttt cagatcgaac attccggtta tctgaaggta
     1201 agcttgaact gataaaatcg gaaatcttga accggcaagt atcaaaggat ttccgaaatc
     1261 agcgagtgac tctataaaaa gaacaagcat ggtgcttgct attcctggaa cggaaagagg
     1321 aagtataact ttgaagaaaa cctgaagcct gttagctttt aagttaaagg ctgcatcttc
     1381 aagggtgggg gatattgtcg atataactcc gtccagagtt ataaacgcta ccgggaaaaa
     1441 cgaaatggtc tgtgctatca taagccccca cagtccatag accgggaagt ttctcacacc
     1501 aaaaattcct gccgttatga ggccgttaaa gccaaaaagc attattatgg aaagtgctcc
     1561 tatgaacggc ggaaatacca taggcataag ggcgacggct ttaaaaaaac gtttaaaagg
     1621 aagtcccgcc ctgtttattg catatgcgta gagaaaaccc agaaaagttc ctatcactgc
     1681 ggtaagcacc gctacaagga tagagttgta aaagccctgt cgtatatatc cgcttttaaa
     1741 aacggtttta tatacttcga gcgagaaatt accgctattg tctgtaaaac ttgtctgcaa
     1801 aacctttata agcggatata ctataaaagt aaaaagagct ataaagataa gtataaccat
     1861 aacaaggact gtcggatttt taagaagagc tttaaaccgg tttttacttt cttcttttat
     1921 caaggtcatc ttttcctcca aaaaaggggg aagttaatcc ccctgttttt attttaccgg
     1981 aagtaccaat tctatccatt tgtcagtaag gcgttttttg ttatctgcgg cccatttctc
     2041 atcaaccgtt ataagcttaa tggttgaaag tggctgcagg tttccgaagt ttacatcgct
     2101 tcttacagga taaaaatagg ttttggcatc cacaattgcc tgctgaccat ttttggacat
     2161 gagccagtca accagcttgg aggcttcctg catatttttg gttccgttca ctattgagac
     2221 acatgccgat tcgtaaggaa cgccgtcttc agggaagact atttctatag gatatccctg
     2281 atctttgaac tcaaagaaag caggagtaaa ctgtattccg atacctgcct gacctactcc
     2341 gacggttttt gatggtcctg ttcctgactg ggtataagac tgaacatttt tagctatctt
     2401 ctttaaatag ttgaaggctt catcctcgcc gtaaatctta accaatccgg ttataaaaga
     2461 gtatgccgtt cccgaagatt gaggattcgg atactgtatc atattggtat atacatattt
     2521 ggtgagatcc tgccatgttt ttgggacagc tgcttttatc tgttccataa ccttaaggtt
     2581 aactcctatt cctattgggt tcatataaaa agtgtgatag tatccttcgc cgtcataaaa
     2641 atcctggctt atattttcaa ttttgaggtt gtaaggcgta gtaagtttct tttcttttgc
     2701 aagaatgtgg tttgacatcg gagcgccgaa ccacacgtct gcctgtggat tggtcttttc
     2761 agcttcaagc cttgttaaag ccggtccgga agaaagaaaa acaaacttta cctttacacc
     2821 tgtttcttcg gaaaatttgt taagaatctt tcgtgcattt gcctcatcca cgcttgaata
     2881 aactataagc tctgcaaaca aagcacttac agaaagaagt attcctaaaa atgctaaaaa
     2941 ctttttcata tcagtattgc cccctttttt tagagtttat atagatttta acggaaaatt
     3001 ctaataaaaa tctttttgaa atgtattaaa attgtaataa aaaacatttt tgtaataaaa
     3061 aagaattttc cgtaataaaa acgaaaaatt ctctattaaa aggcttttat tgactgtaga
     3121 caatactata ttactttaaa gactgttctt ccgatatttt ttccagtata tctttgaaat
     3181 aacttattac agggttattt tcactgccgt agtagtaccc gtctattctc agatctttgt
     3241 taagaataaa gctgctggtg ttgttgtcaa gaaaataaaa ctctcctgtt actgactgtt
     3301 ccccatcaga agggatggta ttttcggtat agtaaggaag agatctgatt ctggaccata
     3361 aatctattat tacctcagga ttctccaact tatacttttt tccatccata ctgaactcaa
     3421 ttttgagagg gatactctcc tcaagcctgc tggaagatga ctgtaccgaa tctataaaaa
     3481 caggtctgtc aaacttttca tatattgatt tgagaccgaa gaaaaaaatt agaaacagaa
     3541 caagaaaaaa gttcatggct gccaagaaaa gttttttatt cagacagacc atctccttta
     3601 cgcaaagaaa gagttacacg tgtatagttg ttctcctcgc taatgatatt tatacttccg
     3661 ttctgttttt tcataatctc tctggcaata aaaagaccca gtccggaact cttcttctga
     3721 ttttttccgg tagtatagaa aggctcaaaa attttttcta ggtcatcttt acttatgcca
     3781 tcgccgttgt ctatgatatc cagaagatat gtgtcttttg atgattctgt gtttatgtgt
     3841 atcacggtgc agtcgctgtg ttttatgcag ttatcaagta tattcatgag tacttcctga
     3901 gtctttgcat aatcggcaag tacgtaagaa ttttccaaag ctgcttcagt ttttatgccg
     3961 aacttgtcaa gtcttggttt tagtgcctga atacactcaa ggacggcttt ttttatatcg
     4021 aaaacggaaa gattcagttc aaactcattc ctcccaaggt ttgacatata aagcatttct
     4081 tctacaagct caagaagtct tttcccctgc tggtttatat atccagttgc tgtatcaaaa
     4141 tcctttttat ctttaatctt gggcagaata tcagagtatc ctattatgga ggtgagagga
     4201 gttttaagct catgtgctac attatcaaca aatcttttct gcttctgttt ttcctgctta
     4261 agattatcta tgtatttgct gagtttttcg gccatgacgt taaaggaact gcttaaatcc
     4321 tgtatctcat cgttagaaga aatagaaatc cttgcattga aagtcccttc agcaattttg
     4381 tccgcttcat ccttaagctt tacgagaggt ctggttattg aataagcgta aaaatagctt
     4441 attactccgc ctgtcaaaag agaaacaact ccaagaagca gaatgacgta tgtgttttta
     4501 taaagaagct ccatttcctg tacgagagga tatacatatc tcaaacatcc gatcacttcg
     4561 ttttcatgat agatgggaca ggatataaaa agtgtctgtg ttccgccggt gttttttatt
     4621 atgtaggttc tttttccatt cgaagcatag tatacatcct gatatacata atacaaattc
     4681 tttgggtaag atatacccaa aagatctttt ctgttattat aaatctcagt ctgaatacca
     4741 agcttatttc ctatgtatgc acaaagaaaa ggactctttg aaacgaatga atcgagtgcc
     4801 tgtgaaagtg tcttgttggc aataaaaccg gagatatact tttgataaaa ggaaagatcg
     4861 gagttcaaag actcaaccgt cttgtttatg accgtagagt acaatgtgtt ggttattatg
     4921 aaaaatatgg ctgaaaagag aagaataaga atcatggcat tctggataat tatttttgat
     4981 ctgagggaaa aacgcatctt attgaacctc aaacttatat ccgatgccat ataaagttac
     5041 aatactctca ccgtgtattc ctagcttttt acgaattctc tgtatcagag catcaagtgc
     5101 acgtgtgtct atactgctgt cataacccca cacctgtgta agaagttctt ctctcctgaa
     5161 tacagttccg ttatttttta caagcaggaa cagaacatca aactccttct ttgtcagata
     5221 cacttcgcta ccgtctataa aagcctttct tgcagactga tccatacgta gaaaaccact
     5281 gctcagtacg catgattttt gagaatgatt tctgtccatt cttttttcaa tggcctttat
     5341 tctgagaatg agctctctgc tgtcaaaggg ctttgatata taatcgtcag ccccgcagta
     5401 aagacccata agtctgtcat ttatctggct tttggcggtc aacaggataa cgggtgtatc
     5461 tacaccgcta cttcttagtt ctttaagaag gttcagtccg tcgtatatgc ccaacattac
     5521 atcaaggatt ataaaatcag gattttcgtg ttcgatgacc gatgaaaaat ccttaccgct
     5581 gtctagggtt attacgtcat aattttcaag tatcaaggac atgcgtacaa gatcaagtat
     5641 gctctgctca tcgtcaacaa caagtatttt catattgtct ccttcggctt ttaatatcca
     5701 cattaattat accgtaaagg agaatttttc gtaacttttt ggtttaagag aaactatgca
     5761 taggctcagt tactatccgg aactgttctg actatacgga agaagccgcc tagatcgatg
     5821 tagcgatctt catcagattc agactgcttg gattttgaac ttcttctttt tacagtaaat
     5881 gaaaaggtat agtcactgta agtttcatat gtcgatttac ctcataaaaa tatggtaaga
     5941 tttattatag tggattgtta taaaaaataa aaagacgagt atatagaaaa taattgttaa
     6001 atcaaaagta tgaaaggaga gctgaaatga agaaagctat tacagtgatg gttcttgtac
     6061 ttcttatact ggctgtacct gtaaaaagtc aggagatatt gcaagatcaa aacattgaaa
     6121 tggttctggt ggaaaaaggc agttttctga tgggaaattt atttatagag ggagacgaaa
     6181 atgaaaaacc attacacaac gtaactttta catacgattt ctgtataggc aagtatgaag
     6241 taacctttga ccagtatgac atatactgtg aggagacaaa aaaagccaca ccgtatgaca
     6301 ttgggtgggg cagaggcaac agacctgtaa ttaacgtaag ctggtacgat gctataggat
     6361 attgtaactg gctgagtgaa aaggaagggt tggcaaaagc atatgacagt gagggaaagt
     6421 ttcttgactc aaacggaaat ataaccacag atccaagtct ggtcgaagga taccgtcttc
     6481 ctacagaagc agagt
//
LOCUS       DAOSHR010000117         6472 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_10450_length_6472_cov_5.053140, whole genome shotgun sequence.
ACCESSION   DAOSHR010000117 DAOSHR010000000
VERSION     DAOSHR010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 6472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6472
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10450_length_6472_cov_5.053140"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="PLU28_07245"
     CDS             <1..328
                     /locus_tag="PLU28_07245"
                     /inference="COORDINATES: protein motif:HMM:NF019903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOG34894.1"
                     /translation="IEIDKNIKVNNIISAVLSEFRLEKQELYSNSRKKSVAQARQLLA
                     YMLKVYTKLNVNEISKELKRNHSTISQSIKKVEQSLLVGNNILKSQLDEIKTKIEEED
                     LDFNVV"
     gene            509..1549
                     /gene="tdh"
                     /locus_tag="PLU28_07250"
     CDS             509..1549
                     /gene="tdh"
                     /locus_tag="PLU28_07250"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HOG34895.1"
                     /translation="MRAVIKKERAPGLTFQMMEEPQIVNPQDVKIKILKASICGTDLH
                     IYKWDQWSQERIKPPQIGGHEFVGKVMEIGPAVKGVKPGDYVACETHIPCGVCYLCKT
                     GNMHVCSDMKILGVHRDGVFADYVVVPEVVLWKVDKSIPLEYASVMEPLGNAIHTLSY
                     VDLRAKNVLITGAGPIGAMAIAVAKISGAASIMVSEVSEYRRNMAQSLGADLLINPAQ
                     ESLQDKVKSFTNGHGADVLIEMSGNVEALNSGMNAVRNCGQISILGVFKDARIPFEMN
                     TAVFKNLSIFTVTGRKMFETWHQAEQWLKYRKIDISKVVTHSFDLEDFEKGFELMASG
                     KSGKIVLNINKD"
     gene            1564..2748
                     /locus_tag="PLU28_07255"
     CDS             1564..2748
                     /locus_tag="PLU28_07255"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HOG34896.1"
                     /translation="MDLIKELCDEMNQLKENGLALNIRTLQTAQGPWLEIENKKVLNM
                     CSNNYLDFANRSELKEAAKKAVDEWGVGPGAVRSIAGTLTIHEKLEKELADFKNVEAT
                     LVLQSGFNANQAVIAPITGENDAILSDELNHASIIDGVRLSKAKRYVWAHKDHKDLEA
                     QLQKAKSDGARRMLVITDGVFSMDGDIAPLPQISEVCKKYEAMLMVDDAHGEGVLGKS
                     GRGIVDHFHLHGQVDIEVGTLSKAFGIVGGFVAGKKGLIEYLKNKARPFLFSSSLSPA
                     ETGAALAALEILKKSDEPVRRLWENADYFKTEMKKMGFDIGNSVTPITPVMLYEASTA
                     KEFSKKLFEEGVFASSIGFPTVPKGKARIRVMISAGHSKEDLKFALSKFEKIGREMKV
                     IK"
     gene            2873..4741
                     /gene="mnmG"
                     /locus_tag="PLU28_07260"
     CDS             2873..4741
                     /gene="mnmG"
                     /locus_tag="PLU28_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HOG34897.1"
                     /translation="MEYNKDMIFDIAVVGAGHAGIEAGIICAKMGMKTLILNINLDMV
                     GWASCNPAIGGPAKGVVTREVDVLGGVMARVTDDTMINVRMLNTSKGIAVRALRAQID
                     KYEYSREMKRILESTDNLYLRYGVVREILTESGKVKGLVTEFGMKYDCRAVILTTGTF
                     LGGKLFIGREVQYGGRLGELPANGLTKNLMDHGIKMERFKTGTPARIRKDSIDFSKMD
                     IQETSSEPLAFSFFSKKKILSPEYPCWLTRTNNNTHDVIRKYISFSPLYGDVKLVQGK
                     GPRYCPSIEDKVMKFDKQSHQIFVEPESRFSQEYYLNGLSTSLPIEAQIGIIRSINGL
                     ENAVIERPAYAVEYDYACPDQLFPTLESKKIENLYFAGQINGTSGCEEAAGQGLVAGI
                     NAAAKLKGMKPLILRRDEAYIGVLIDDIINKSVDEPYRLLTSRSEFRLLLRHDNAHLR
                     LAKYGYEYGLISQDFYRTVQEIEKEVNENIQRISRINVYPSAVNDILTLYSTEPIEHT
                     MKLGDILKRPQIPYEALKQFDSIPIQNDEIAEQTDIYFKYGGYLKKMEEEVKKMKKME
                     EIIISDSFDYDQVHNLAFEAREKLKKIKPATIAQAMRIPGINPSDILNLSVYVNRK"
     gene            complement(4794..6266)
                     /locus_tag="PLU28_07265"
     CDS             complement(4794..6266)
                     /locus_tag="PLU28_07265"
                     /inference="COORDINATES: protein motif:HMM:NF014073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HOG34898.1"
                     /translation="MDYFLSVFNLRFILILFSGSLIGLIIGALPGLSVTMATALLLSV
                     TYSWPFYDAIALMIGVYVVGVYSGAISSILVNIPGTPASVATSFDGFPMSCKGNSKKA
                     LWIATVYSFIGTVFGLIVLSLIAKPLSKIALSFSPIDYFLLAFFGLTTVGSLTSKNIL
                     KGHISAFIGVMISMVGIDSIMGVPRFTFGSLNLQLGIPIVPVLIGLFGFSEVLEQISS
                     QTSSQISLLNNNERVSIKEILKYWKISLISSAIGTIIGILPGAGAPVASLLAYDSAKK
                     YTKKPKIPFGQGAPEGIAASESANNACIGGALVPMLTLGIPGDAVTAVMLSAFYVHGL
                     RPGPLFIKENPQAFELIVLSTLIGSFFIIILGFLFIPVLSKVVLVPKKIMLPLIAILC
                     IVGAYSVNSSLYEVSIMTVFGVIGFFMRKTGFSTAAMVLGIVLGEMMDSNFRRALSLS
                     GSVRELITGNFFKPISIVIIVFIVLSVFLSIKNKTNAAKK"
BASE COUNT         2320 a          982 c         1348 g         1822 t
ORIGIN      
        1 catagaaata gataaaaaca tcaaggttaa taatatcatc agtgctgttc tgtcagagtt
       61 caggcttgaa aaacaagagc tttattctaa ttctagaaag aagtctgttg cacaggcaag
      121 acagctactg gcatatatgt taaaagtgta caccaagttg aatgtaaatg aaatttcaaa
      181 ggagctgaaa aggaatcatt caaccataag ccaatctatt aaaaaagttg agcaatcact
      241 gcttgttggc aataatatat tgaaaagtca gctcgatgag ataaaaacaa aaattgaaga
      301 agaggatttg gatttcaatg tagtataagg tgccgtctat tcggtgcctt tttttttata
      361 gttgttcaaa tagctggaat aaatcaaaat agatgcttca aatcaccatt ttttaactaa
      421 taatataaga cataggcatt gaaatttttt tcattatgta ctaaacttta tatatagaga
      481 gaaaaaaacg atataaagga gagatactat gagagctgtg ataaaaaagg aaagagctcc
      541 tgggcttaca tttcaaatga tggaagaacc tcagattgtt aatccgcagg atgtcaaaat
      601 caagattctt aaagcttcta tatgcggaac cgatttacat atatataagt gggatcaatg
      661 gtctcaggaa agaataaagc ctccgcagat tggaggacac gagttcgtag gaaaggtaat
      721 ggaaataggt cctgcagtaa aaggagttaa gcctggtgat tatgttgcct gtgaaacaca
      781 cattccttgc ggagtatgct atctgtgtaa gaccggtaat atgcatgtat gtagtgacat
      841 gaaaatacta ggtgtacaca gggatggagt ttttgctgat tatgttgtag ttcctgaagt
      901 ggtactttgg aaagtagata aatctattcc cttggaatat gcttcggtta tggaaccttt
      961 agggaatgca attcatacgc tttcgtacgt tgatctcaga gctaagaacg ttcttataac
     1021 cggtgccgga cccataggcg caatggcaat agcagttgca aagataagcg gagcggcatc
     1081 cataatggta agcgaggtat cggagtatag gagaaatatg gctcagtctt tgggagcaga
     1141 tcttctgata aatcctgctc aggaaagtct ccaggataaa gtaaaatcat ttactaacgg
     1201 acatggagca gatgttctaa ttgaaatgtc tggaaatgtt gaagctttga attctggaat
     1261 gaatgctgtt cgtaactgtg gtcaaataag catattggga gtatttaaag atgcaagaat
     1321 accttttgaa atgaacacgg ctgtattcaa aaatcttagt atttttactg tcacaggaag
     1381 aaaaatgttt gaaacatggc atcaggctga acaatggttg aaatatagaa aaattgatat
     1441 aagtaaggtt gttacccatt cctttgatct tgaggatttt gaaaaagggt ttgaactgat
     1501 ggccagtggg aaatcaggaa agatagtgct caatatcaat aaagactgat gaagggggat
     1561 aaaatggatt taataaaaga actctgtgat gagatgaatc aattgaagga gaatggtctc
     1621 gcacttaaca taagaacatt acagacagct cagggaccgt ggctggaaat agaaaacaaa
     1681 aaagtactta atatgtgttc aaataattat ttagactttg ctaacagatc cgagttgaaa
     1741 gaggcagcta aaaaagcagt tgatgaatgg ggagtaggac ccggagcagt caggtccata
     1801 gcaggaacgc ttacaatcca tgaaaaactt gaaaaagaac ttgcagattt taagaatgtt
     1861 gaagctacat tggttttaca atctggattc aatgctaatc aagcggttat cgctcctata
     1921 actggagaaa atgatgcaat tctatcagat gagcttaacc atgcaagtat tattgatgga
     1981 gtaagacttt caaaagcaaa gagatatgta tgggcgcaca aagaccataa agatcttgaa
     2041 gcacagctgc agaaagcaaa atcggatggt gcccgtagga tgcttgtaat aactgacggt
     2101 gttttcagta tggacgggga tattgccccc ttgcctcaga tcagtgaggt atgcaagaag
     2161 tacgaggcta tgctcatggt ggatgatgca cacggagaag gggttcttgg aaaaagtgga
     2221 agaggaatag ttgaccattt tcatcttcat ggtcaggttg atattgaagt aggaactttg
     2281 tccaaagctt ttggtatcgt aggtggattt gttgcaggta aaaaaggatt aatagagtat
     2341 cttaagaata aagctagacc ttttttattt tccagttctc tttcacctgc tgaaaccggt
     2401 gcagctttag ctgcattaga aatacttaaa aaatctgatg agcctgtgag aaggctgtgg
     2461 gaaaatgctg attacttcaa gacagaaatg aagaaaatgg gatttgatat tgggaatagt
     2521 gttactccta ttactcctgt gatgctctat gaagcttcaa cagcgaagga gttcagcaaa
     2581 aagctttttg aagagggagt atttgcttct tcaatagggt ttccaacagt tcctaaggga
     2641 aaggcaagga taagagtaat gataagtgca ggtcattcta aagaagatct taaatttgct
     2701 ctttcaaagt ttgaaaaaat aggaagggaa atgaaagtaa taaaataaat acttaaagat
     2761 tacgcgtaag cgtaatcttt tttttgtgga tgataaacta agttgttaat tagtaacata
     2821 ttaagttaca ttctaatgtt ataatctcct acgaaaaagg aggaagaagt ttatggaata
     2881 taataaagat atgatttttg atatagcagt tgtgggagcc ggtcatgcag gcatagaagc
     2941 cggaataatt tgtgcaaaaa tgggtatgaa aactcttatt ctgaacataa accttgatat
     3001 ggtcggatgg gcctcatgta atccggcaat aggtggacct gccaaaggag ttgttactcg
     3061 agaggtagat gttttgggcg gagttatggc aagagttacc gatgatacta tgattaatgt
     3121 aagaatgctt aatacaagta aaggaatagc tgtaagagca cttagagcac agatagacaa
     3181 gtatgaatac tcaagggaaa tgaagagaat tctcgaaagt acagacaatc tttatctccg
     3241 ctatggtgtt gtcagagaaa ttctcactga atcgggaaaa gtaaaaggat tagtaacgga
     3301 attcggtatg aagtatgact gccgtgcagt gatattaact accggtactt ttttgggagg
     3361 aaaacttttt attggcagag aggttcagta tggtggaaga cttggagaac taccggcaaa
     3421 tggtcttaca aagaatctta tggatcacgg gataaaaatg gagagattta aaaccggaac
     3481 acccgcaaga ataagaaagg actctataga tttttcaaaa atggatattc aagagacttc
     3541 ttcagagcct ctcgcatttt cgtttttttc aaagaaaaaa attttatctc ctgagtatcc
     3601 ttgttggctg acacgcacca ataataatac gcatgacgta ataagaaaat atatatcatt
     3661 ttcaccccta tatggtgatg taaagcttgt acagggaaaa ggtccaagat actgcccttc
     3721 tattgaagat aaagttatga agtttgataa acagtcccat caaatatttg ttgagccaga
     3781 atcaaggttt tctcaagagt attatcttaa cggattaagc acgagtctac cgattgaggc
     3841 acaaataggt attatacgat ctataaatgg tcttgagaat gcagttattg aaagaccggc
     3901 ttacgcggta gagtatgatt atgcttgtcc agatcagttg tttcctacac ttgaaagtaa
     3961 aaaaatagaa aatctctatt ttgcaggaca gataaacgga acaagtggat gcgaagaagc
     4021 tgcaggtcaa ggattggttg caggaattaa tgcggctgcc aagcttaaag ggatgaagcc
     4081 tttgatactc agaagagacg aggcttatat tggagtattg attgacgaca taattaacaa
     4141 aagtgtagat gaaccataca ggttacttac atcacgatca gaatttcggc tacttttgcg
     4201 tcatgataat gcacatttga gacttgcaaa gtatggatac gagtatggtc taatatcaca
     4261 ggatttttat agaactgttc aggaaataga aaaagaggtt aatgaaaata ttcaaagaat
     4321 atcaaggata aatgtttatc catcagctgt aaacgatatt cttacattat actctactga
     4381 gccgattgaa catactatga aactgggaga tatacttaag cgtcctcaaa ttccatatga
     4441 ggctttgaaa cagtttgaca gtataccgat tcaaaatgat gaaattgctg agcagactga
     4501 tatatacttt aaatatggtg gttatctgaa aaaaatggaa gaagaagtaa agaagatgaa
     4561 aaaaatggaa gagataatta tttctgatag ttttgattat gatcaggttc ataaccttgc
     4621 atttgaggca agagaaaaat taaaaaaaat aaaaccagca accattgccc aggcaatgag
     4681 aatccctgga atcaatccat ctgatatatt gaatctttct gtttacgtaa atagaaagta
     4741 acataaaatt ttaatataat cccttttgtt gagtaaaata ttagcaaaag ggattatttt
     4801 tttgcagcat tagttttatt ctttatactt aaaaatactg aaagaactat aaagacaata
     4861 ataactatac ttataggctt gaagaaatta cctgtaatca gttctcttac tgaaccgctt
     4921 aaagataaag ctctacggaa gttagagtcc atcatctctc ctagaacaat tcctaagacc
     4981 atagccgcag tagaaaaacc agttttccgc ataaaaaatc caataactcc aaaaactgtc
     5041 attatagata cctcataaag actcgaattt acagaatatg ctccgactat acatagaatg
     5101 gctattaaag gaagcattat ttttttaggt accagaacta cttttgataa tactggtata
     5161 aaaagaaatc ccaaaataat aatgaaaaag gatcctatca atgttgataa aacaatgagt
     5221 tcaaatgcct gaggattttc ttttataaaa agcggtccag gtcttaagcc atgaacataa
     5281 aaagcagaaa gcattacagc tgttactgca tcaccaggta ttcctagagt aagcataggt
     5341 acaagggctc ctcctatgca ggcattattg gcgctttcac tggcagctat accctcagga
     5401 gcaccttgtc caaaaggtat ttttggtttt tttgtatact tctttgcact gtcataagcc
     5461 aaaagagaag caactggagc accagctcca ggaagaatac ctattatagt tccgatagcg
     5521 cttgatatca acgatatttt ccaatatttt aatatctcct taatagaaac tctttcattg
     5581 ttatttaata gggagatttg agaactggtt tgagatgaga tctgctctag tacttcggaa
     5641 aatccaaaca agccaatcag aacaggaaca ataggaattc ccagctgaag atttagcgag
     5701 ccgaatgtaa atcttggaac tcccataata gagtcaattc caaccatgct gatcataact
     5761 ccaataaaag cacttatatg accttttaga atattttttg aagtgagcga acctactgtc
     5821 gtaaggccga aaaaagcaag aagaaagtaa tctatcggcg aaaatgagag tgcaattttt
     5881 gatagtggct tggcaataag cgaaagaaca attaaaccaa atactgtgcc gataaaagaa
     5941 tatacagttg caatccaaag agctttttta gagttaccct tgcagctcat tggaaatcca
     6001 tcaaatgatg ttgccacaga tgctggtgta cccggtatat tgacaagtat ggaacttatt
     6061 gcacctgagt atacgcctac aacgtatact cctatcatta gagcaatggc atcataaaaa
     6121 ggccatgaat aagtaacaga taaaagcaat gcggttgcca ttgttactga caaacccgga
     6181 agtgctccta taataagtcc tattaaagat cctgaaaata aaataagtat aaaacgcaaa
     6241 ttaaaaactg ataaaaaata atccacgaaa tcaccttatg ggagagctac attaagtatc
     6301 tttccgaata tgaaaaatat aactgctgaa gataaaaatg agatgataag gtttaaaagt
     6361 ttattttttt gtcctgaaag cattgacagt ataaaaacat acaaaattgt cgcataaaaa
     6421 aagtgtatat atggaactaa aaaaataaaa gcaaaggtaa gtataatagt aa
//
LOCUS       DAOSHR010000118         6399 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_10629_length_6399_cov_4.886034, whole genome shotgun sequence.
ACCESSION   DAOSHR010000118 DAOSHR010000000
VERSION     DAOSHR010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 6399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6399
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10629_length_6399_cov_4.886034"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(121..705)
                     /gene="rdgB"
                     /locus_tag="PLU28_07270"
     CDS             complement(121..705)
                     /gene="rdgB"
                     /locus_tag="PLU28_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HOG34899.1"
                     /translation="MEKIKVMLATTSKHKVAEVSEIIKEYAQVYSMQDSPDVEETGKT
                     FIENSVIKAVTVGNMLNQKVMADDSGLCIKALDGFPGIYSARYMSDRSFEEKMQSILT
                     KMDGISDRQAYFACAATFYDPVTLTLVSYEARVYGNISIQIMGNGGFGYDPIFIPQGE
                     TKSFGQLPAEFKNKCSHRAQAFTELMEKIKKIYG"
     gene            complement(708..1355)
                     /gene="rpe"
                     /locus_tag="PLU28_07275"
     CDS             complement(708..1355)
                     /gene="rpe"
                     /locus_tag="PLU28_07275"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOG34900.1"
                     /translation="MVKFFPSLLAANFFDLSGDIRKIEKISDGLHLDVMDGFFVPNIS
                     FGFPVIKSVRENTDMFFDTHLMIESPERYIDRFCELSDAVTVHYESTVHLHKAVSMIK
                     EKGCKAGVSLNPSTPVAVLQEILPYLDTVLIMSVNPGFTGQKFIETSVQKVARLAGLC
                     GILDCNVEIMVDGGVNSGNIQKLYDAGATSFVLGAAVFHADDPQTAIQELRKAVE"
     gene            complement(1349..2251)
                     /gene="rsgA"
                     /locus_tag="PLU28_07280"
     CDS             complement(1349..2251)
                     /gene="rsgA"
                     /locus_tag="PLU28_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOG34901.1"
                     /translation="MSWEKAVVIRFHSNMVSAVDIQTNKRLECVLPGKFKQQHIRPVV
                     GDYIEYSSEENAEYAKVENILPRKNELYRPKIANVDQVVLVTTIKNPSVDNIIVDKFL
                     IQVQKQRLECVIVVNKIDLLQTAQEKELLDRFVAAYSKILKVITVSVKQDIGIEEVRN
                     TLCGKITTTAGMSGVGKSSLINKLDSTLNLKVNEISDRLKRGRHTTTFSELIAFSFGG
                     FLADTPGFANLELRNFECATLKDFFPEFGIYEPYCAFNDCVHINEPGCAVKQAVQEKE
                     ISSERYNNYLSIYREIKESGEKKW"
     gene            complement(2255..2752)
                     /locus_tag="PLU28_07285"
     CDS             complement(2255..2752)
                     /locus_tag="PLU28_07285"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HOG34902.1"
                     /translation="MKKGILKGLIHILMVIFGLFAAGILSILIMMVYTSSAKKVEVPY
                     LIGETKNVGIQALKEKGLIPVVDGTGDTVLYTDPKAGVKVKAGHHVLIQLRSIDIQKV
                     PDLIGVPLEVADQFLVQYKITYTVQKIRTSDSTKHGIVLEMSPDVGEELNKNAIKLII
                     GVYEG"
     gene            complement(2733..3797)
                     /gene="rlmN"
                     /locus_tag="PLU28_07290"
     CDS             complement(2733..3797)
                     /gene="rlmN"
                     /locus_tag="PLU28_07290"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOG34903.1"
                     /translation="MTKKNILDFEYEELEVFLKNELNIEKFRTKQVADWIYKKHVFTF
                     SEMTNLSQQVKDKLEERLYIGIPEIVDMQVSKVDGTIKFLLQFMDGAKVESVIMYYAN
                     RVSACISSQVGCPLKCSFCWTGKGGFTRHLTSGEIVSQVLAMEKAKELNINNIVFMGM
                     GEPLLNYENTMKSIRIMNEENFRNIGARHITISTSGIADKIEELGDVKLDIRLSLSLH
                     APNNQKRDRIMPVNSKYPVEEVMQACRNYQKKTKNRVTIEYTLIRNFNETKEDALELA
                     TLLEGLKVMINLIPVNPNPKGYERPSRPFMIKFAEELRNKGFEVSLRAERGTDIDAAC
                     GQLRKRNIERTEVRDEKRNS"
     gene            complement(3790..5091)
                     /locus_tag="PLU28_07295"
     CDS             complement(3790..5091)
                     /locus_tag="PLU28_07295"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOG34904.1"
                     /translation="MERELAYKLLRQYEKKETISNESVKQARNMLDERSFGLFKSLVW
                     GSIRNIAKEDWLISKFVDKPKDLTPASLWLLRLGFYQLISGFDEHAAVYETVALVSSK
                     KSKSFINAVLRNYIRAKDTLGNEPENVKYLLPKWLYEYIRKRHTFSSSIIKFNTSTPR
                     RSIRVNSLKTSREELLQMLADSKIAAQTAKYSPYGIYIDEDSLALEETELYRKGYFYI
                     QNESSQLIPIILNPQIGEQLLDLCSAPGGKCTFSAELMKNTGSIKAVDNDISRLESVS
                     ANCKRHGITNVSVQLNDASEFRDAFEYDKLMLDVPCTSLGTAYAHPEVLFRVKKEDFE
                     RYAQIQRKILTNAMSNIKVKGEIAYSTCTLSWEENTGNMKWLKKNFNVSFLDLRERLG
                     SLGVKFHFDGYGAYIFHDEGLIPFYIAMFRKETLKTEEKND"
     gene            complement(5094..5780)
                     /locus_tag="PLU28_07300"
     CDS             complement(5094..5780)
                     /locus_tag="PLU28_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HOG34905.1"
                     /translation="MFWYDPSFLILIPAFFLTLWAQISVQSTFNTYSRKRSALGETGA
                     AFARRLLDSAGLYDVKVEFVNGFLSDHYDPSSKVLRLSKSTYGSLSVAALGVVAHEVG
                     HAIQHAKNYQPLMIRNISVPLANMGSTLSWVIFFMGLIFSTPLLLKAGIFLFIFVVLF
                     TLITLPVEFDASKRAIKMLPVVGMPSSEVDEAKKVLNAAAMTYVAAAFMAISQLLRMV
                     LIAGAVDRRD"
     gene            complement(5816..>6399)
                     /locus_tag="PLU28_07305"
     CDS             complement(5816..>6399)
                     /locus_tag="PLU28_07305"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOG34906.1"
                     /translation="RINAQNIAKYASNKKVLDAFAYTGNFGIHSLKGGAKFVTFIDYS
                     ERALKILEETLKLNKFSAERYELVNANTFDYLRVLDDTSRYYDITVIDPPSLAKSRDS
                     KANAIKGYKELNLRAMKITRSGGYLCTSSCTQIVYEEDFRKIIFEAAQDTGKKLTVVY
                     RGMQSPDHPLIYNIFETEYLKHYFIRVENLEEF"
BASE COUNT         1836 a         1407 c         1079 g         2077 t
ORIGIN      
        1 atgtctgttc tatcaaaaaa actcagtctg atacttaatc agttcaaaac ctgaaagtac
       61 ttacatataa tttaaatgca cccctttttc attgaaagct gaagtggggt gcattccatt
      121 ttatccgtat atttttttta tcttttccat aagttctgta aaagcttgag ccctgtgact
      181 gcatttattc ttaaactcag ccgggagctg cccgaaagat ttcgtttcac cctgaggaat
      241 gaaaatggga tcgtacccga atcctccgtt tcccataatt tgaatgctta tatttccgta
      301 aacacgggct tcatacgaaa ccaacgtaag cgtaacagga tcataaaaag tggcagcaca
      361 agcaaagtat gcctgtcggt cacttatacc gtccatcttg gtcaagatac tctgcatctt
      421 ttcttcaaaa gacctgtctg acatatatct ggcagagtat atcccaggaa aaccatccag
      481 cgcttttata caaagccctg aatcatcggc cattactttt tggtttaaca tgtttcctac
      541 agtaacagct tttatgacag agttttctat aaaagttttt cctgtttcct caacatcagg
      601 actgtcctgc atagaataca cctgagcata ttctttaata atctcagata cttctgcaac
      661 cttgtgcttg cttgtcgtgg caagcattac tttaattttc tccaaaatca ttctaccgcc
      721 tttcgtagct cctgaattgc ggtctgcgga tcatctgcat gaaacaccgc tgctccaagg
      781 acaaaggatg ttgctcctgc atcgtagagt ttttggatat tcccggagtt gactcctccg
      841 tctaccatta tttcaacatt acagtccaat atcccacaaa gtcctgctaa ccgtgctact
      901 ttctgaacag aagtttctat gaacttctgt cccgtaaagc ctggattcac actcattata
      961 agcactgtat ccagataagg aagtatctcc tgaagaaccg caaccggagt tgaaggattc
     1021 aaagaaacac cggctttaca gcctttttct tttatcatgc ttactgcttt gtgaagatga
     1081 actgtcgatt cgtagtgaac tgtaactgca tcagatagct cacagaatct atcgatatat
     1141 ctttctggac tttctatcat aagatgagta tcaaaaaaca tatctgtgtt ttctctgact
     1201 gactttatta caggaaatcc aaaggatata ttcggaacaa aaaatccatc catgacgtcc
     1261 aaatgaagtc cgtcggatat tttttctatt tttctgatat caccggacag atcaaaaaaa
     1321 tttgccgcca gcaaagaagg aaagaacttt accatttttt ctcaccactt tcttttattt
     1381 ctctgtatat gctcaaatag ttattatacc tttcagagga tatttctttt tcttgaacgg
     1441 cttgcttaac tgcacaaccg ggctcattta tatgaacaca gtcgttaaat gcacagtacg
     1501 gttcatatat tccaaactcc gggaaaaaat ccttaagtgt cgcacattca aagtttctga
     1561 gttcaagatt tgcaaaaccg ggtgtatctg caaggaatcc gccaaatgaa aaggctatga
     1621 gctcggaaaa ggttgtagtg tgtctgccgc gtttaagcct gtctgatatt tcatttacct
     1681 tcaaattcaa agtactgtcg agtttattta tcagacttga ttttccgact ccggacattc
     1741 cggctgtagt agttatctta ccgcaaagag tattccttac ttcttcaatc ccaatatctt
     1801 gcttaacaga taccgttatg actttaagta tttttgagta tgctgccaca aacctatcaa
     1861 gcagttcttt ctcctgtgct gtttgcagaa ggtctatttt gtttaccaca attacacact
     1921 caagccgttg tttctgaacc tgaataagaa acttatcaac tataatgttg tctacagaag
     1981 gattttttat tgtagttaca agcaccacct gatcgacatt ggctattttg ggtctgtaaa
     2041 gttcattctt cctcggaagg atattctcaa cctttgcata ttcggcattt tcctcagacg
     2101 agtactctat ataatctcct acaaccggcc gtatatgctg ctgtttaaac ttacctggca
     2161 agacacactc taaacgttta ttcgtctgaa tatccaccgc tgaaaccatg ttggaatgga
     2221 accttatgac aaccgctttt tcccaggaca ctcgtcaccc ctcataaaca ccgattataa
     2281 gttttatagc atttttattc aattcttctc caacatccgg agacatttca agaacaatac
     2341 cgtgcttggt actgtcactg gttcttatct tctgcacagt ataggttatc ttgtactgaa
     2401 caagaaactg atctgcgact tcaagcggaa cccctataag atcgggcact ttttgtatat
     2461 caatacttct tagctgtata agaacatgat gaccagcttt tacttttact cccgctttag
     2521 gatctgtata aagaacggta tctcccgttc cgtctacaac aggtataagg cctttttctt
     2581 taagagcctg gatgccgaca ttcttcgtct cacctataag ataaggaacc tcaacttttt
     2641 ttgccgagct ggtataaacc atcattataa gtatcgaaag aattcctgcg gcaaaaagcc
     2701 caaaaataac catcagaata tggatgagcc ctttaagaat tccttttttc atcacgcacc
     2761 tctgtacgtt ctatgttgcg ttttctaagc tgaccacaag ccgcatcaat atcggttcct
     2821 ctctctgctc ttaaactgac ttcaaatcct ttatttctaa gctcctctgc aaactttatc
     2881 ataaatggtc tcgaaggtct ttcatatcct ttaggattag gattgaccgg tatgaggttt
     2941 atcattacct tcaacccctc aagaagcgtt gccagttcaa gcgcatcttc cttggtttca
     3001 ttgaaatttc ttataagagt gtactctatg gtaactctgt ttttggtttt tttctggtag
     3061 tttctgcagg cttgcataac ctcttcaaca ggatactttg aatttacggg cattattcta
     3121 tcccttttct gattattagg agcatgaaga gaaagagaaa gccttatgtc aagttttaca
     3181 tctcccaact cttctatctt atcggctatt cctgatgtag aaatagttat atgccgtgcg
     3241 cctatatttc tgaaattttc ttcgttcatt atccttatgg atttcattgt attttcataa
     3301 ttcaaaagag gctcgcccat tcccataaaa actatattat ttatgttaag ctcttttgcc
     3361 ttttccatag caaggacctg tgaaacaatc tcaccgctgg taagatgcct tgtaaatcct
     3421 ccctttcctg tccagcaaaa gctacactta agaggacatc ctacctgtga agatatacag
     3481 gcagaaactc tgttggcata gtacatgatc actgattcca cttttgctcc gtccataaac
     3541 tgaagaagaa actttatggt gccgtctact tttgaaacct gcatatccac aatctccgga
     3601 atgcctatat agagacgttc ctccaatttg tccttgacct gttgagaaag attggtcatc
     3661 tctgagaatg taaagacatg ctttttgtat atccaatcgg caacctgttt ggttctgaac
     3721 ttctctatgt taagttcgtt ctttaaaaaa acttctaact cctcgtattc aaaatcaagt
     3781 atatttttct tagtcatttt tttcctccgt ttttagcgtt tcttttctga acattgcaat
     3841 ataaaaaggt atcaatcctt cgtcatggaa tatgtacgca ccatatccgt caaagtgaaa
     3901 ctttactcca agactgccga gtctttctct cagatccaaa aaacttacat taaagttctt
     3961 cttaagccat ttcatgtttc ctgtgttttc ttcccacgat agagtgcaag tcgaataagc
     4021 tatctcccct ttaaccttta tattactcat agcattggtg agaatttttc tctgtatctg
     4081 agcatatctt tcaaaatctt cctttttaac cctgaaaagc acttcaggat gagcataagc
     4141 agtaccaaga gaagtacatg gcacatcaag cataagttta tcatactcaa aagcatctct
     4201 gaactcggaa gcatcattga gctggacaga aacatttgta atcccatgtc tcttacagtt
     4261 ggcagaaaca ctttccagtc ttgaaatatc gttatctact gcctttatac tgccggtatt
     4321 tttcataagc tcagcagaaa aagtacattt tcctcccgga gccgaacaaa gatcgagcaa
     4381 ctgctctcct atctgcggat ttaagataat aggaatcagc tgcgaggatt cattctgtat
     4441 ataaaaatag ccttttctgt aaagttccgt ttcctccagg gccagagagt cttcatcaat
     4501 ataaatacca tatggagagt attttgctgt ttgtgcagct atttttgaat ctgcaagcat
     4561 ctgcaaaagc tcttcacggg aagtttttaa tgaattaacc cttatgcttc tgcgtggcgt
     4621 ggatgtatta aactttatta tggaagaaga aaaagtatgt ctctttctta tatattcata
     4681 aagccatttc ggaagaaggt acttgacatt ttccggttca ttgcccaaag tatcctttgc
     4741 tcttatataa ttcctgagaa cagcatttat aaaagatttg cttttcttac tggatacaag
     4801 tgcaactgtc tcataaactg ccgcatgctc gtcaaaacct gatattaact gataaaaccc
     4861 cagcctcagc aaccaaagcg aggccggagt aagatcttta ggcttgtcaa caaacttgga
     4921 gatcagccaa tcctctttgg ctatatttct tatgcttccc cagaccaaag atttgaaaag
     4981 gccaaaactc ctttcatcaa gcatatttcg tgcttgtttt acactttcat tggatatggt
     5041 ttccttcttt tcatactgcc tgagcagttt atatgccagt tctctctcca ttgttaatct
     5101 cttctgtcaa cagcacctgc aatcaaaacc attctcaaaa gttgtgaaat agccatgaat
     5161 gctgctgcaa catatgtcat agcggctgca ttaagtacct ttttggcttc atctacttca
     5221 gaagacggca ttccgactac cggaagcatc tttatagctc ttttgctggc atcaaactcc
     5281 accggcaacg ttataagggt aaataagacc acaaaaataa atagaaatat accggcctta
     5341 agcaacagtg gagtggaaaa tataagaccc ataaaaaata tcacccatga aagcgttgaa
     5401 cccatgttag caagaggaac agaaatattt cttatcatca agggctgata gtttttagca
     5461 tgctgtatgg cgtgtccgac ttcgtgggca actacaccca aagccgccac agataaagaa
     5521 ccatatgtgg attttgaaag tctaagaacc tttgaagatg gatcataatg gtcagaaaga
     5581 aaaccattca caaactctac ctttacatca taaagtccgg ctgaatccag cagcctcctg
     5641 gcgaatgcag cacctgtctc tccaagagcg gatctttttc tggaatatgt attgaatgtc
     5701 gactgaactg atatctgtgc ccaaagagtc aggaaaaatg caggtataag aatcaaaaac
     5761 gacggatcat accagaacat aatattcacc tccgatttat tttttttaat tattttcaga
     5821 actcttcaag gttttcaact cttataaaat aatgtttaag atactcagtt tcaaatatat
     5881 tatatatgag aggatgatcg ggagactgca tgcccctgta tacaacggta agcttctttc
     5941 ctgtatcctg tgccgcttca aagataatct tcctgaaatc ttcttcatag acaatctgtg
     6001 tacatgaaga agtacagaga taacctccgc tgcgggttat cttcatcgct ctcagattaa
     6061 gctccttata gccttttata gcgtttgcct tggaatctct ggacttggcg agggatggag
     6121 gatctattac agttatatcg taatatctgc tggtatcatc aagaactctc agataatcaa
     6181 aagtattggc attgacaagc tcatatcttt ccgcagaaaa tttgttcagc ttaagggttt
     6241 cttcaagtat tttgagagct ctttctgaat aatctataaa agtaacaaac ttagctcctc
     6301 cctttagaga atgtatgcca aaattgcctg tataagcaaa agcatctaac acttttttgt
     6361 ttgatgcata tttggctatg ttctgtgcat tgatacgct
//
LOCUS       DAOSHR010000119         6351 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_10761_length_6351_cov_5.198062, whole genome shotgun sequence.
ACCESSION   DAOSHR010000119 DAOSHR010000000
VERSION     DAOSHR010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 6351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6351
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10761_length_6351_cov_5.198062"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1398
                     /locus_tag="PLU28_07310"
     CDS             <1..1398
                     /locus_tag="PLU28_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34907.1"
                     /translation="IVVFTTLLLMRLPAFTQMSVMTIAGLFVFFIVMVFVVPAIMTIF
                     KSSLKVSRIEEYTNKFFKKMSYFIPKNGKIIVRIVVPIMIIFSIIGAFSTLNFSYTTP
                     GLVSASSESSKIGVLVLSKMGQTSFDSLKCAVRFDENIDDFKSRLLATGAVKSIESLP
                     DTIEKQVGKYADLKNKVQGLSEVIANPMVASILKKYNIYSDSLKIIDIISRSSDLNQF
                     SLNILEILPQELKSSFFMEKDGVKYIILSIQPAFDIWSNNGLKKFFDKIGKDNTDRIL
                     GNPKAMYKIMSIVSKRFILPTAVSFIFIAIFTYFSRKKNIKETLLTFFGLVSASLGTF
                     GVGYFMGIRPTFTTLLTYPLVSGVGVEGFIHIFHAIDEDPKHYWQSLNSVTFSIATTI
                     AAFISFNVSEGALLKQFATTMALSLVFSWVASAVLIPAINRKKIKEYWKKEKDMREEI
                     AEVEKNFEKDMEKQE"
     gene            complement(1575..1937)
                     /locus_tag="PLU28_07315"
     CDS             complement(1575..1937)
                     /locus_tag="PLU28_07315"
                     /inference="COORDINATES: protein motif:HMM:TIGR03987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsmA family protein"
                     /protein_id="HOG34908.1"
                     /translation="MLVFSVVLITLALICYSIGVWSEKIQGSLRAKHLVFFWLGFIFD
                     SSSTLIMGTISRSSSVSLHSISGIAAILLMLLHAVWASIVLLSGKEEKKKNFSRFSIL
                     VWCLWLIPYSTGAVMNML"
     gene            complement(1978..2523)
                     /locus_tag="PLU28_07320"
     CDS             complement(1978..2523)
                     /locus_tag="PLU28_07320"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOG34909.1"
                     /translation="MPKTIEDVENLILKNAFELLKTYGYHKLNMREIAKKSNIATGTI
                     YNYFPTKDELMISLIRFYWKDYGDKMDAAFSSEEDFLVKMHRIFEIIDTFIRTFEGFI
                     PEYAKKGNEKPEGRVQKFLNEFRKGIKNALDMDKTISIVPSIKTEELSAFIAANMISI
                     ALYRLYTYENFEKIVRIIILK"
     gene            complement(2678..3832)
                     /locus_tag="PLU28_07325"
     CDS             complement(2678..3832)
                     /locus_tag="PLU28_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34910.1"
                     /translation="MKKALVFLSVIALLVMITSCSAPAKQKTYFELQQELYKQNRTSD
                     VAIAPSVETVVVEGIGIDKDDALLDARLKALQQVVGLKLYSQSQVSNFTLVDKQTYTK
                     TQGYIKSEKILSESAFQISYYKVKCSFEVSKDIPDDDFFFVIKKMRKPKVGVWIPANL
                     YKGLLSFEDRSAEVNLLNRLKEYGFDVYDTERLQELMQIKADFTPDVKSFRKYGVDVL
                     ITGELFGESTGSISGLMGARATVNLKVFWTQTGQIIASDSVTEGKADVSAAIAVKNAV
                     GKAAYTIGESLSRLIVKDWMSQVANGLPLEMNIMNIDYDSYVDFVSMLRKASGVIDLG
                     STVFEQNTADIVVYTFLLPEEFYAKIIRTSFKNSPTMINQSLTQITFKLN"
     gene            complement(3837..4820)
                     /gene="dprA"
                     /locus_tag="PLU28_07330"
     CDS             complement(3837..4820)
                     /gene="dprA"
                     /locus_tag="PLU28_07330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOG34911.1"
                     /translation="MNTLDIIALKEVNKLKNREVIDMVDNNRDFLVIRTDFSQRKDEI
                     VHRLSDVQGLKILTYWDSDYPQIIRNIPDPPAVLYCLGDISLLNSECLSIVGSRKTTE
                     YGRQICKNLCSQLDDHTLVSGMAFGIDTIVHQNAKRTIAVLGCGVDVIYPQSNKELYR
                     RLVEEELVISEFFPGTPPAKYTFPYRNRIIAGLSSKTIVVEASKKSGSLITAAYAANF
                     SRDVYAVPGDITRLNSEGTNHLIYSGATPITSVERARKILGLQEKLVLGCSAYENGES
                     VLIGYIRKGISTLDELAEKTGRDLSELMAEISEHEINGLIYQESGKYYYKG"
     gene            complement(4894..5256)
                     /locus_tag="PLU28_07335"
     CDS             complement(4894..5256)
                     /locus_tag="PLU28_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOG34912.1"
                     /translation="MVKKAVIGKAEDIQPVIMNNDKVKNVEKRVLIGKKIGAGNFVMR
                     LFKVGPDGHSPRHSHVWEHEIYIIKGTAGIFDGEKEVAAPEGSYVFVPGGVEHQIKNI
                     GTGEMHFLCLIPSTADEE"
     gene            complement(5262..5921)
                     /gene="jag"
                     /locus_tag="PLU28_07340"
     CDS             complement(5262..5921)
                     /gene="jag"
                     /locus_tag="PLU28_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HOG34913.1"
                     /translation="MIKLDIKQYTAKTAELAYENALNEYKVPKEELSFTVVQEAAKTG
                     FLGLRTIPAIIEVYLNENHLLANLQDFLNIILDKFEGTFYSNLKIFGKTIVVSLEGEG
                     LEKLIGKYGRTLGALQHLLMIYVNRMTDTKIDVKLDIGEYRKNRKKHIEEIADSTARK
                     AIREGSTELAPMFSFERKIIHEYIRKNFPQLETKSVGLEPYRKVVVYAKELQTTGSKK
                     R"
     gene            complement(5927..>6351)
                     /locus_tag="PLU28_07345"
     CDS             complement(5927..>6351)
                     /locus_tag="PLU28_07345"
                     /inference="COORDINATES: protein motif:HMM:NF014186.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HOG34914.1"
                     /translation="GFNPASGCLFALIQLPIFIVLWQVIQYFGESFAYNPRFLIWQDL
                     SAGGWAENWLFIIISLIAYIVNAMLSSQDVKAAWQTILMSLLFPFMLIGLPSGVFIYY
                     TLNAILQVGITLLVNFLYKSKGIGFRDLFGLGPKPVRR"
BASE COUNT         1906 a         1332 c         1066 g         2047 t
ORIGIN      
        1 attgtcgttt ttacaactct tctgctaatg agactaccgg cattcacaca gatgtcggta
       61 atgaccattg cgggtctttt cgtattcttt attgtcatgg tttttgtggt tccggctata
      121 atgactatat tcaaatccag tcttaaggta agtagaatag aagagtatac caataaattc
      181 ttcaaaaaga tgagctactt tatacctaaa aatggaaaga taattgtaag gatagtagta
      241 ccaataatga ttattttctc tataatagga gctttttcaa cccttaattt ttcatatacc
      301 acacccggtc ttgtatcagc cagttcggaa tcttccaaaa tcggagttct tgttctgtca
      361 aagatgggac agacctcatt cgattcgctt aaatgtgcag tacggtttga tgaaaacata
      421 gacgatttta aaagccgtct gcttgcaacc ggagctgtaa aaagcataga aagcctgcct
      481 gataccatag aaaaacaggt ggggaagtat gccgacctaa aaaataaagt tcaaggattg
      541 tccgaagtaa ttgccaatcc aatggttgca tccatactga aaaaatataa tatctacagt
      601 gacagtctga agataattga tataataagc cgctcgtctg atcttaacca gttctcctta
      661 aatatactgg agatactacc tcaggaactt aaaagctcct ttttcatgga aaaagatggt
      721 gtaaaataca taatccttag tattcagcct gcttttgaca tatggagcaa taacggtctt
      781 aaaaaattct tcgacaaaat aggaaaagat aatacagaca gaatattggg aaatcccaaa
      841 gccatgtata agataatgag cattgtcagc aagagattca tacttccaac ggcagtgtct
      901 tttatcttca tagccatatt tacatacttt tcgagaaaaa agaatataaa agaaaccctt
      961 cttaccttct ttggacttgt aagtgcttcc ctaggaacct tcggtgtagg ctattttatg
     1021 gggataaggc cgacttttac cactcttctg acctatccgc ttgtatctgg tgtcggcgtg
     1081 gaaggcttta tacatatatt ccatgcaatc gatgaagatc ctaaacatta ctggcagtcc
     1141 ctcaactctg taacattttc tattgctaca actatagccg cttttataag tttcaatgtc
     1201 tctgaaggcg ctcttctgaa gcagtttgct acaactatgg ctctttcact tgtcttctca
     1261 tgggtagcaa gtgccgtgct aataccggca attaaccgta aaaagataaa agagtactgg
     1321 aaaaaggaaa aagatatgcg tgaagagata gcagaagttg aaaagaattt tgagaaagat
     1381 atggaaaaac aggaataaaa aagtcggatc ctcaaggatc cgataatggt tttaaactat
     1441 ctcaggggga tagaagtatt tcctatctgt ttataatgat aacatcatca ttattactat
     1501 tcaacttaag ttagtaaaaa aattatttcg ttaagttaac tatccgataa ggaaattttt
     1561 atcgagaaat ttttttaaag catgttcatt acagctcctg ttgaatacgg aataagccat
     1621 agacaccata ccagaatgct gaatctgctg aaattcttct ttttttcctc cttgccgctc
     1681 aagagcacta tgcttgccca tacggcgtgt aaaagcatta aaagtatcgc ggcaattccg
     1741 gaaatactat gcaggcttac agatgatgaa cggctgattg ttcccataat caaagtggac
     1801 gaactgtcaa atataaatcc taaccaaaaa aacacaagat gttttgccct gagagaaccc
     1861 tgaatttttt cgctccatac accaatgcta taacatatca gagcgagcgt aattaacact
     1921 accgaaaata ccagcattct ttttcctcct caatttttaa ctgcttatct ttcgttttta
     1981 cttcaaaata attattctta ctattttttc aaagttttca taagtataaa gacgatataa
     2041 ggctatgctt atcatattag cagctataaa ggctgagagc tcctctgttt tgatgctagg
     2101 aacgatactt atcgtcttat ccatatcaag agcatttttt ataccttttc ggaactcgtt
     2161 aagaaacttt tgtactctgc cttccggttt ttcattacct ttttttgcat actccggtat
     2221 aaatccttcg aaagttctta taaaagtatc aattatctca aatatcctgt gcattttcac
     2281 caaaaaatct tcttcagaag aaaaagcagc atccatcttg tctccgtaat ctttccagta
     2341 aaatcttata agacttatca taagctcatc tttagttgga aagtaattat agattgttcc
     2401 ggttgcaata ttggattttt ttgctatctc acgcatatta agcttgtgat acccatatgt
     2461 ttttaaaagt tcaaaggcat ttttcagtat taaattttca acgtcttcta tcgtctttgg
     2521 cattttctgt cacctcactg aatattttga tagacatcgt gtatattttt atgaaccatg
     2581 ttcataaaaa tatacagttt tttctttgca tgcatatatc tttacgatta agtattgaca
     2641 taaaaaagcc tgatctctta agagaccagg ctttttgcta attaagcttg aaagttatct
     2701 gagtaagtga ctgatttatc atagttggag agtttttgaa ggaggtcctt attatctttg
     2761 catagaactc ttcggggaga aggaaggtat aaaccactat atctgctgta ttctgttcaa
     2821 aaactgttga accaagatct ataacgccgg aagccttcct gagcatagat acaaaatcta
     2881 catagctgtc ataatcaatg ttcattatat tcatttcaag aggaagacca tttgcaacct
     2941 gactcatcca gtccttgaca ataagtcttg aaagactttc tcctatagta taggctgctt
     3001 ttcccactgc attttttact gcaattgcgg ctgaaacatc agccttacct tctgtgactg
     3061 aatcagaggc aataatctgt cctgtctgtg tccagaatac tttgaggttt accgtcgctc
     3121 gtgctcccat aagaccgctt atactgcctg tgctttctcc aaaaagctct ccggttataa
     3181 gaacatccac accgtacttt ctgaatgact tcacatccgg ggtaaaatca gcttttatct
     3241 gcataagttc ctgaagcctt tcggtatcat aaacatcaaa accgtattct ttaagcctgt
     3301 taagaaggtt cacttctgca gatctgtcct caaaactcaa aagacctttg taaaggttag
     3361 ccggtatcca gactccaact ttaggctttc tcattttttt aattacaaag aaaaaatcat
     3421 cgtccggaat atctttgctt acttcaaatg aacattttac tttataataa cttatctgga
     3481 aagcactttc actgagtatc ttttcgctct taatatatcc ctgtgtctta gtataagtct
     3541 gtttatccac aagggtgaag ttagatacct gtgattgaga gtaaagttta agcccaacta
     3601 cctgctgaag cgcttttaat cttgcatcta agagtgcgtc atccttatca attcctattc
     3661 cttcaaccac aactgtctct acagaaggtg caatggctac gtcagatgtt ctgttctgct
     3721 tatacagctc ctgctgaagc tcaaaatacg ttttttgctt tgccggagca ctgcaggaag
     3781 taatcataac caatagagct atgaccgaaa gaaaaactaa agcttttttc atcttatcac
     3841 cccttataat aatattttcc tgattcctga tagatcagac cgtttatctc atgttcagat
     3901 atttctgcca tgagctccga aagatcccgg ccggtttttt ctgcaagttc atcaagggtt
     3961 gaaatgcctt tacggatata ccctataaga acagactctc cgttttcgta cgcagaacag
     4021 cctaaaacta acttttcctg taaacctaaa attttccgtg ctctttccac ggaggtaata
     4081 ggtgttgcac cagagtatat gagatggttt gttccctccg aatttaatct ggttatatct
     4141 ccgggcacgg cataaacatc tctggaaaag tttgcagcat atgctgcggt tatgagcgaa
     4201 ccgcttttct tagatgcttc cacaactatt gttttacttg aaagacctgc aattatccta
     4261 tttctgtaag gaaaggtata cttagcagga ggtgttcccg gaaaaaactc ggaaataacc
     4321 agttcttcct ctaccaacct tcggtaaagc tctttattgc tctgcggata aataacatcc
     4381 acgccgcagc ccaaaactgc aatagtccgt ttggcatttt gatgtactat tgtatctata
     4441 ccaaaagcca taccgcttac aagtgtatgg tcatccaact gtgagcatag gtttttgcat
     4501 atctgccgtc catactcagt tgttttcctg cttccgacta tcgaaagaca ctctgaattt
     4561 agaagcgaaa tatctcccag acagtaaaga acagcaggag gatcaggtat attacgtatt
     4621 atttgaggat aatcactatc ccagtatgtc agtatcttta atccttgcac atcggaaagc
     4681 ctgtgtacta tctcatcttt cctttgactg aagtcagttc tgataaccag aaaatcacgg
     4741 ttgttgtcca ccatgtctat aacttctctg ttcttaagct tatttacttc cttaagcgca
     4801 atgatatcca acgtattcat aaaatcatcc cccgtgcaat tatatcatgt acagcctatt
     4861 aagaaaaatg cgcagtataa actgcgcatt tttttattct tcatcagctg tgcttggaat
     4921 aagacaaaga aagtgcattt ctccggttcc gatatttttt atctgatgct ccacaccacc
     4981 gggcacaaaa acataacttc cctcaggggc cgcaacttcc ttttctccat caaatattcc
     5041 tgctgttccc ttaataatat agatctcatg ttcccataca tgggagtgtc tgggagagtg
     5101 tccgtcggga ccaaccttga aaagcctcat aacaaaattt cctgcaccaa ttttcttacc
     5161 tataagtact cttttttcaa catttttcac tttatcgtta ttcattataa caggctgaat
     5221 atcttcggct tttccgataa ctgccttttt taccatagaa atcacctctt ttttgaacct
     5281 gtggtctgaa gctcctttgc atacactaca accttcctgt atggctcaag accgacagat
     5341 ttggtttcaa gctgaggaaa attctttctt atgtactcat gtattatctt tcgctcaaat
     5401 gaaaacattg gtgcaagctc ggtactccct tcccttatag ctttacgtgc cgttgaatcg
     5461 gctatctctt ctatatgctt tttcctgttt ttcctgtact cgcctatatc aagctttaca
     5521 tctatcttgg tatctgtcat cctgttcaca tatatcatga gaagatgctg aagagctccc
     5581 aaagttcttc cgtacttgcc tataagtttt tctagtcctt ctccttcaag cgatactact
     5641 atggttttac cgaatatctt aagattagaa taaaaagttc cctcgaattt atctaaaata
     5701 atatttaaga aatcctgaag gttggccaaa agatgatttt cattcagata tacctctatt
     5761 atggcaggaa tagtccgcaa acctaagaat ccagttttag ccgcctcctg tacaacagtg
     5821 aatgaaagct cctcctttgg aactttatac tcgttcaaag cattttcata tgccagttcg
     5881 gcagtctttg ccgtatactg tttgatatct aactttatca agcatttcac ctcctcacgg
     5941 gtttaggacc aagaccgaac agatccctga aacctatgcc ttttgactta tataagaaat
     6001 taacaagcaa tgtgataccc acctgcaaaa ttgcatttag agtatagtat ataaaaacac
     6061 cggaaggcaa gcctatgagc atgaacggaa acaaaaggct cataagtatg gtctgccaag
     6121 ccgcttttac atcctgggaa gaaagcattg catttactat ataagctatc aagcttatta
     6181 ttataaatag ccagttttct gcccatcctc cggcagaaag atcctgccag ataaggaatc
     6241 tggggttgta agcaaagctt tctccgaaat actgtataac ctgccaaagc acaataaata
     6301 tcggaagctg tattaaagca aaaagacaac cgctcgcagg attgaaccct t
//
LOCUS       DAOSHR010000120         6279 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_10937_length_6279_cov_6.422076, whole genome shotgun sequence.
ACCESSION   DAOSHR010000120 DAOSHR010000000
VERSION     DAOSHR010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 6279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6279
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10937_length_6279_cov_6.422076"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(19..285)
                     /locus_tag="PLU28_07350"
     CDS             complement(19..285)
                     /locus_tag="PLU28_07350"
                     /inference="COORDINATES: protein motif:HMM:NF015662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34915.1"
                     /translation="MVDSRGKLLYLIFAIGILILLTRAFYLQVFKWSTLNFEVQDLNT
                     RIIQSDSQRGSIYDRNGKLLAWNEKLFQVYNLSEEIDKQTGTYT"
     gene            complement(275..688)
                     /locus_tag="PLU28_07355"
     CDS             complement(275..688)
                     /locus_tag="PLU28_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34916.1"
                     /translation="MNILLSFLVAVSAIVSSSWDKWMGSQLVFSMPFLAVYFKHLDMN
                     DEKRFIFAIVYTLFYFANRYDIGFMAILFFVLYFLCDLFLSKFKHNFWTALLYFSMLS
                     FYLNFLSFSFFSFFTGIILISILYFLNLRLITHGR"
     gene            complement(692..1351)
                     /locus_tag="PLU28_07360"
     CDS             complement(692..1351)
                     /locus_tag="PLU28_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34917.1"
                     /translation="MKKKLFFLNLFIILAIFFNLIKSAENIFYWISYPVDFLFSKVQQ
                     KFLSAGISADSFDKILETIESSPIRVVDTMEIKLSIPYGIIMSDDLTSFTVVANKKPK
                     LNSLVIDENANLLGFVCGVFSNRVEVKKLGYGKNEFFGEIEGLDVLIKESEGNILVEL
                     PENFTFESTQVYINIPKYLLSVSESSTLMTGEFVSQYYANFFLFRSKKASNPVVYFLE
                     E"
     gene            complement(1354..2367)
                     /locus_tag="PLU28_07365"
     CDS             complement(1354..2367)
                     /locus_tag="PLU28_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOG34918.1"
                     /translation="MRAYLGIDLGTANTLVYLKGKGIIINEPSVIAIDKVSEDILQVG
                     TEAKKMIGKTPANIVAIRPLRDGVIADYEVAMAMLKYFINQALGGFNLIKPTVIVGIP
                     TNATEVERRALKNATLDAGAGKAFLIEESMATAIGAGLEVEEASGNMAIDIGGGTTEI
                     AVISLGNIVLSKSIRIAGDELDQAIVNYIKAKYALLIGDRTAEQIKIEIGNCFETPEY
                     NNLKIDVIGLDMLSGLPKRVVLSGIEIREAISLPVSKVTENIKLAIENTPPELLADIV
                     NKGIFLAGGGAMLKGMKELIEKETKIRVVVAEEPLTCVARGAGLVVDKINILENLEKN
                     RAI"
     gene            complement(2473..4638)
                     /locus_tag="PLU28_07370"
     CDS             complement(2473..4638)
                     /locus_tag="PLU28_07370"
                     /inference="COORDINATES: protein motif:HMM:NF019353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34919.1"
                     /translation="MNNLRKDFSGSVISIDLPKVLFPARIMKGFFKEPDGLALSDTLD
                     DQFQKKLKLFESYETAYSIDTFKVQFPDLGGINALINILKGKKFSESEIKRLRYELEV
                     VKKLNVSNYILNVKNIVDTAKKNQISVGPGRGSAVGSFLIYSLGITQVNPLSHDLLFE
                     RFLNFYRKEMPDVDIDVDARFRNKLILELSKFYGKNRVSEIRTYSRYKTKSALKRAGE
                     VIGNLIETSFLDFPLRSPSNIKKLGSFSRDQKILFYISYYLEGLHSARSTHAAGIVIS
                     EKDIAEALPIQAGAVPVIEWEMKELAFAGIEKFDILSLDTITFAKDLNIDYTKIEKKD
                     EKTFSEISKGFTAGLFQLDSYTGRKLSVKIRPRDFDELTVLLSINRPGPLDSGMVEEY
                     VSDSSPDFLRKIFPNTHGVLIFQEQIMFLAQNIANFSIEQSDMLRSALSKKNTSKILS
                     LKKDFLMNASLKIGKDDAQRLFGYIENFAQYSFPKSHAVAYSYLTYNLAREKFVNPEK
                     FFLCLIKYKGLSESIINEILALGMNIVAPDLFFPQGGIVQKDFILPMSCIKGIPQAID
                     LRTPQTTDFIGIFEYLENIGLNRVVIENFIRAGAFDKVDFNRKRLLRQMTAITQGRIS
                     ELEDIKNSIFGDIKSSEKKEKNISEEELILYETESLGYPITVIKQAGLPKNIYKKFFD
                     PSRKIEFYGYHYLKMLIDSSGIYFIRNFSKVPTKIFLRF"
     gene            5042..5317
                     /gene="rpsO"
                     /locus_tag="PLU28_07375"
     CDS             5042..5317
                     /gene="rpsO"
                     /locus_tag="PLU28_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOG34920.1"
                     /translation="MSRGLDVDIKKDIIEQFRINEKDSGSTEVQIALLSARIKHITEH
                     LKKHPKDYHSRKGLTSMVGKRRKMLNYLKSERPEVYQDLLKKLGIRG"
     gene            5424..>6279
                     /locus_tag="PLU28_07380"
     CDS             5424..>6279
                     /locus_tag="PLU28_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOG34921.1"
                     /translation="MKYWEKEIFGRKIRIETGKMAKQSLGSVLINFNESSLLVTANAN
                     EKSKPDVDFMPLTVEFQERFYAIGRFPGGFMKREGKPSDEAILASRLIDRPIRPLFPN
                     DFHNEVQIISTVFSMLPDDSIETWGIAGASLALNISPIPFEGFVAGVRIGYVDGNYIA
                     FPTQEQLKNSRMDVIVAGTKDAVTMVEGESLEVTEEEMVKAIMFAHDAIKQIISIQEE
                     IRSEIAVEKWVIKNIEYPSEFISDFKSIMNDDLLKKAILTKGKKARDNAISEYKEKVF
                     NDFEEKYLE"
BASE COUNT         2113 a         1121 c         1074 g         1971 t
ORIGIN      
        1 tcactttttt taagtatttc acgtataagt tcctgtttgt ttgtcgattt cttcactcaa
       61 attatatact tgaaagagtt tttcattcca tgccagaagc tttccgtttc tgtcgtatat
      121 acttcctctt tgcgagtcag actgtattat tctggtatta agatcctgaa cttcaaagtt
      181 tagggtactc catttgaaaa cctgaagata aaaagctctt gtcaaaagta taagtattcc
      241 tattgcaaaa ataagataaa gtaacttacc tctgctatct accatgagtt atcagcctca
      301 gattaagaaa ataaagtatg gagatcaaaa tgatccctgt aaaaaaggaa aaaaaggaaa
      361 aactcagaaa gttaagataa aaagagagca tggaaaagta tagtaatgct gtccagaaat
      421 tatgcttaaa ttttgacaga aaaaggtcac acaaaaagta caaaacaaaa aaaagaattg
      481 ccataaagcc tatatcgtac ctgtttgcaa aataaaacaa ggtataaaca attgcaaata
      541 taaaacgctt ttcatcattc atatcgagat gtttaaaata aacggctaga aaaggcattg
      601 aaaaaactaa ttgcgatccc atccatttat cccaggaaga acttactatt gcagatactg
      661 caactaaaaa agatagtaaa atattcatgg cttattcctc caaaaaatat accacaggat
      721 tggatgcttt ttttgatcgg aacaaaaaga aattagcgta gtattgtgaa acaaactctc
      781 ctgtcatcag agtgctgctt tcagacacag aaagcaggta ctttggtatg tttatataaa
      841 cctgagtaga ttcaaaagta aaattctcag gaagctcaac aagaatattc ccttcggact
      901 cttttatcag aacatccaag ccttcaatct ccccgaaaaa ttcatttttc ccatagccta
      961 gtttcttaac ctcaactctg tttgaaaata ctccgcatac aaaacccaga agattggcat
     1021 tttcatcgat taccagagag ttaagctttg gctttttatt ggctacgaca gtaaaagaag
     1081 taagatcatc ggacattatt atcccatagg gaatggaaag ctttatttcc atagtatcta
     1141 ccactcgtat aggtgaagat tcgattgttt caagtatttt atcaaagctg tctgcggaga
     1201 tgcctgccga taaaaacttc tgctgtactt ttgaaaataa aaaatctacc ggataagaaa
     1261 tccagtagaa aatattttct gcactcttta ttagattaaa aaagatagcc agaattataa
     1321 aaagatttag aaaaaaaagt ttttttttca tttttagata gctctatttt tctcaagatt
     1381 ttcaaggata tttattttat ctaccacaag acctgctccg cgagctacgc aagtcagcgg
     1441 ttcttcggca acaacaaccc gtatcttcgt ttccttttcg atcagttctt tcattccttt
     1501 aagcatagct cctccgcctg caaggaagat tcctttattg acaatatctg ccaatagttc
     1561 tggaggtgtg ttttcgatgg ctaattttat attctccgtt acttttgaga ctggaagcga
     1621 tatagcttca cgtatctcta tacctgaaag gacaacacgt ttaggaagtc cgctgagcat
     1681 atcaagaccg ataacatcaa ttttaagatt attatattca ggagtttcaa aacagttccc
     1741 tatttcaatt tttatctgct cagcagttct gtcgcctatc agtagtgcgt actttgcctt
     1801 aatgtagttg acaatagcct gatcaagctc atcaccggcg atacgtattg attttgaaag
     1861 aactatgtta ccaagcgaaa ttactgctat ttcagtagtt cctccgccga tatcaattgc
     1921 catatttcca gaagcttctt caacttcaag tcctgctcct atggcagtcg ccatagactc
     1981 ttctattaaa aaagccttcc cagccccagc atcaagtgtt gcatttttaa gcgcccggcg
     2041 ttcaacctca gtagcattgg ttggtattcc cactatcacc gtaggtttta taaggttaaa
     2101 accaccaagt gcctggttta taaagtattt cagcattgcc atggcaactt cataatcagc
     2161 aataactcca tctctcaaag gtcttattgc aactatattg gctggtgtct ttccaatcat
     2221 tttttttgcc tcggttccaa cctgaagaat atcttccgaa actttatcta tagcaataac
     2281 cgatggctca ttaattatta ttccttttcc ctttaagtat acaagtgtat tggctgttcc
     2341 aagatcaatt ccaagatatg ctctcattca aaaaatcctc cctgtcatgt catcaatata
     2401 aagtataaca cacttactgt aatacggata agtcgaaaat gattagaaac agaaaattaa
     2461 atgatcttct gtttaaaacc taaggaaaat cttagtagga acttttgaaa aatttcttat
     2521 aaagtatatt ccagaagagt caataagcat tttcaaataa tgatagccgt aaaactcaat
     2581 ttttctggat ggatcaaaga actttttata aatattttta ggtagaccag cttgttttat
     2641 aacagttatg ggatatccta agctttcggt ttcataaagt ataagctctt cttcagaaat
     2701 atttttttct tttttctctg aagatttgat atctccaaat atactgtttt taatatcctc
     2761 aagctcactt attcttccct gtgtgatggc tgtcatctgt ctgagtaatc tttttctgtt
     2821 aaaatccact ttatcgaaag caccggctcg gataaaattt tctatcacaa ctctgtttag
     2881 tccaatgttt tcaagatact caaagattcc tataaaatct gtagtttgag gagtcctcag
     2941 atctatggct tgagggatgc cctttataca actcataggg aggataaaat ctttttgaac
     3001 aatacctccc tgagggaaga aaagatcagg agcaacgata ttcataccca gtgcaagtat
     3061 ttcatttatt atagactcag atagtccttt gtattttata agacaaagaa aaaatttttc
     3121 aggattgaca aatttttctc tggctaagtt gtaggttaga taggagtatg caacagcatg
     3181 agatttggga aatgagtact gggcgaaatt ttcaatgtag ccaaaaagcc tttgagcatc
     3241 atcttttcca atcttcaaag atgcattcat taaaaaatcc tttttgagac tgagtatttt
     3301 tgaagtgttc ttctttgaaa gagcagaacg gagcatatca gactgctcaa ttgaaaaatt
     3361 agcgatgttt tgtgctaaaa acattatctg ttcctgaaaa atcagtacac catgagtatt
     3421 tggaaagatc ttccgcagaa aatcaggaga actatcggaa acatactcct ctaccatgcc
     3481 ggaatcaagc ggaccgggtc tatttataga taaaagaacc gtaagttcat caaaatctcg
     3541 tggtctgatt tttacggaaa gcttgcgacc tgtatatgag tcaagctgaa acaaaccagc
     3601 cgtaaagcct tttgatattt ccgaaaaagt tttctcgtct tttttttcaa ttttagtata
     3661 atcaatattc agatcttttg cgaaagttat tgtatccaaa gatagtatat caaatttttc
     3721 aatacctgca aaggcaagct ctttcatctc ccactcaata acaggaaccg ctcccgcctg
     3781 aatcggcagc gcttcagcaa tatctttttc agatattact atacctgcgg catgtgttga
     3841 cctggcgcta tgaagacctt ctaaataata tgatatataa aataatattt tctgatccct
     3901 tgaaaaagaa ccaagttttt ttatattaga aggactcctt aacggaaaat ccaggaaaga
     3961 agtctcaata agattcccaa taacctcacc ggccctttta agtgccgatt tggtcttgta
     4021 tcttgagtaa gttcttattt ctgagactcg gtttttccca tagaactttg aaagctccaa
     4081 gatcaattta tttctgaacc ttgcatcgac atcgatatct acatcaggca tttcctttct
     4141 atagaaatta agaaatcttt caaacaaaag atcatgagaa agaggattta cctgagttat
     4201 tcctaaggaa tatattaaaa aagatccgac tgccgagcct cttcctggtc ctacggaaat
     4261 ttgatttttt tttgccgtat caactatatt ttttacatta agtatatagt tcgaaacatt
     4321 aagtttctta actacctcca gctcgtatcg taaacgtttt atctcggatt ctgaaaactt
     4381 ttttcccttt aagatgttta taagagcatt gataccgccc aggtctggaa actgaacttt
     4441 gaaagtatca atactatatg ctgtctcata agattcaaag agtttaagtt ttttctggaa
     4501 ctgatcatca agagtatcag atagcgccaa accgtcaggt tctttaaaaa atcctttcat
     4561 aatgcgagca gggaaaagca ctttaggaag atcaatactg ataacgctac cgctgaaatc
     4621 ctttctcagg ttattcataa accttatgac agagagcaag tcatcggagt tgcgcattaa
     4681 aaaaatctgg tttttgtatt caaacagtat aatgggtcta aaaccgtttt tatgggacag
     4741 atataagaac tcagcccacg aaaaaacgtg atcctcacag agaatcacag tattaatttg
     4801 atgtttttta cagaaataaa aaacatctgt taattgaagg taagactttc ctaagctttt
     4861 tgatgtaaca actggtccaa gtagtataaa atcacctcca atcgatatta tatcataatc
     4921 aaaagagata aaagagtaca ttgacataaa tctcagaatt tagtataatt aatttacctt
     4981 aactcagtaa aaggaatatt cctttacttg gtttttggac taaaatcagg aggtaagtca
     5041 aatgtcaaga ggtttagacg ttgatatcaa gaaagatatc atagaacagt ttagaattaa
     5101 cgaaaaagac agtggttcca cggaggttca gatagctctg ctttcagcaa gaataaagca
     5161 tataaccgag catcttaaga agcatcctaa agattatcat tccagaaaag gtctgacaag
     5221 catggttgga aaaaggagaa agatgcttaa ctatctaaaa tctgaaagac ctgaagttta
     5281 ccaggatctt ttaaagaaac tcggaataag aggctaatca gaggtgggaa tcttcccacc
     5341 ttatttttat cattatatgt taaatgttct aaaactatgg taaaataggt ttatagagaa
     5401 aataaattcg tgaggtgaaa taaatgaagt attgggaaaa agagattttt ggtagaaaaa
     5461 ttagaataga gacaggaaag atggccaaac agtcgttagg atcggtttta ataaatttta
     5521 atgaatcttc gttgcttgtt acagccaatg caaacgaaaa atcaaaacca gatgttgatt
     5581 ttatgccgct tacagtagaa tttcaggaaa gattttacgc aatagggaga ttccccggcg
     5641 gatttatgaa aagagaaggg aaacccagtg atgaggctat tcttgcttca cgacttattg
     5701 atagaccaat aagaccattg tttcctaatg atttccataa tgaagttcag attatttcaa
     5761 cagtgttttc aatgcttcct gatgatagta ttgaaacatg gggaatagct ggggcttctc
     5821 tggcacttaa tatatctcct atcccatttg aaggctttgt tgctggggtt agaataggct
     5881 atgtagatgg taactatatt gcatttccaa cacaggagca gcttaagaac agcagaatgg
     5941 atgtgattgt tgcgggaaca aaagatgccg ttacaatggt tgaaggggaa tcacttgaag
     6001 taactgaaga agaaatggtt aaagcgatca tgttcgccca tgatgcgata aaacagataa
     6061 tatcaatcca ggaagaaata agaagtgaaa tagcagttga aaaatgggtt attaaaaata
     6121 tagaataccc ttccgaattt ataagtgatt ttaaatccat tatgaatgat gacctgctta
     6181 aaaaagccat acttactaaa ggtaaaaaag cacgtgacaa tgctatatca gagtataaag
     6241 aaaaggtttt caacgatttt gaagagaagt accttgaaa
//
LOCUS       DAOSHR010000121         6211 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_11100_length_6211_cov_4.754224, whole genome shotgun sequence.
ACCESSION   DAOSHR010000121 DAOSHR010000000
VERSION     DAOSHR010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 6211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6211
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11100_length_6211_cov_4.754224"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(107..1297)
                     /locus_tag="PLU28_07385"
     CDS             complement(107..1297)
                     /locus_tag="PLU28_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34922.1"
                     /translation="MKKFWFFAAIFLASFTLVFSNDAENQVISFRDPRAIFSHPTERD
                     NFIRYMAVMYNKGYNLSFESVKSISEGAGYTYDDFVLSYYKYFIGELNLVQVKDLKTY
                     VSVSTVTAVATPTAVSKPVETKPVEKQPEVLNYRDDVKEYVSRIFESFTDARSNCKSS
                     GMLDYFDEMVPVDNTYYRELYKLKYNPILRDDYPERTDLVFRMNKLMDYYFELSASSF
                     KSWFAYIFPNKEFTKQDINAYSKYLVDVAYAYSNSFLTFYSPVVPMESFFKQKVIVYD
                     VPPELILAFLVAESRLAPFSFRVEQKGNYAYAVSMGLSHILIDADLLDIDYPEFGNKN
                     VDRYTFDFLSSYYLGNTFGKEDIFSDMDLLTVRGSSLYCSIMLELIYQRLYDYYYGVY
                     FLGQ"
     gene            complement(1316..>2131)
                     /locus_tag="PLU28_07390"
     CDS             complement(1316..>2131)
                     /locus_tag="PLU28_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34923.1"
                     /translation="TLDSIKTRSEDRTEDKYDSRVADYIKEIYSKLSGSLSNQNSDGI
                     FNKFDDEVPVKANEYRTDHKNNFNPPLIPFSNRTFLVTRVKDLLDYYKTSSEFFKPHF
                     AKVFTESVDTFDTQKYSEMLVDLSYAYIGSNLSFTSSIDNSFFRSSVIIKDIPVEMVL
                     AFFVQESRLLPGSFRAEISSNKNIYAVSFGLAHTLIDADFLEIAAAHDDIGNNDAGGR
                     NFDIISSRYLGNTAKQKTYFSDYDLLTVRGSLLYSSIFMQLLYQKLITYKDSF"
     assembly_gap    2132..2231
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2232..2496)
                     /locus_tag="PLU28_07395"
     CDS             complement(<2232..2496)
                     /locus_tag="PLU28_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34924.1"
                     /translation="MLINVIKKPEEENFKGLTKTQEEKLVRYAYTAYNKGYNYTFEQI
                     KGFADNPSYTADDNVLAFYKYLNGEVTLDSIKTRSEDRTEDKYD"
     gene            2696..4648
                     /locus_tag="PLU28_07400"
     CDS             2696..4648
                     /locus_tag="PLU28_07400"
                     /inference="COORDINATES: protein motif:HMM:NF019912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34925.1"
                     /translation="MNKNHIPIMIVLVFMCQFLYAGDVKYVYTPAGPEGMIIYDASDI
                     SQMKVVSTFNLGGYVKDLVPGEKVVYAIADNVGVICIDISNVLKPRIIGKLNFADVLR
                     KIEISTEKAYIDYYDSILDKSGIAVIDIKDPRNMKFLYEIFEEDYVSSYVVNGDYIYL
                     SDGREGLKVIQAYEDTYKLVSRYKINSSKLFYNNNMLFAGEINSAIYIFDVTDPKKLL
                     FKSKYSFPRDFSELAVDGDRLFVLCKEQGLYMFKVNYESGVNYLTKLYSDDIKTFHIS
                     GNMLLLLINSDLDREDGLVVLDISDIYSPSIIKQDFSFINGEAKDIVVYGKYAYIADS
                     NKGIKIYDVSNVKKSKYVKTIDCTGSFEALFLEGKRLYAMLDRSGISIYDLSDPLTPK
                     KLGNVAEATLKNSIYLKADMLFMSQGNTVKVYNVSDPENIKQISSITDISEVNNGEII
                     KNIRISDKYLILSGYRTNSGGIKSGFIKIFDISEVEKPLCIKRFQDIGIGWVSPVSSP
                     YFDRYYLYMGLTDIQGYERMKTFTVLDIFDMRTMKVIGSADYEDNIGKKYTIGVQDLE
                     IYNNYALLACGEDGIKIFNIRDPKKPFYVTSVKTGGFASSIFVKSNYAYVADGENGFF
                     ILDIRNPNNPIISYDALWINMKKIKS"
     gene            complement(4674..5321)
                     /locus_tag="PLU28_07405"
     CDS             complement(4674..5321)
                     /locus_tag="PLU28_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HOG34926.1"
                     /translation="MEKPYPQESRLFDLVSPQEFKVPSLSLTELLKNRKSRRAFKNTP
                     LSLNELSFLLWSTQGIKEIIRNGYASLRTVPSAGARHAFETYLYIRNVEGFEEGVYRY
                     LPIEHKLFLSYTQPDLSQKIIEGCYGQHMAGESAVTFIWSVIPYRMEWRYAESSYKLL
                     LLDAGHVCQNLYLACENITCGTCAIGAYSQKLMDNFIKADGKEEFVIYMAPVGKY"
     gene            5565..6164
                     /locus_tag="PLU28_07410"
     CDS             5565..6164
                     /locus_tag="PLU28_07410"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOG34927.1"
                     /translation="MKTPDRKTAHDILNQASEMNPGKWIEHSKNTAYAAEKIARHIKG
                     LDPEKAYTAGLLHDIGRREGVRNLHHIICGYEYMMSLEYDGTAKISMTHSFALKNLNT
                     YLGEYDCTDAELDFIRTYIENVQYDDYDLLIQLCDCLALADGLCIIEQRLVDVAMRYG
                     FNELTIPKWKKIFEIKTYFEKIMGTSIYDVLGIMPRQMK"
BASE COUNT         2109 a         1044 c         1103 g         1855 t
ORIGIN      
        1 ttaccaacgt ttaaaatcct ttttgaaaat gcgttttagt tgcttttcga caatgaacat
       61 cactgcatca tctgcttctt cttatatata cataaacaga ataaacttac tgaccaagaa
      121 agtaaacacc ataataataa tcataaagtc tctgataaat aagttcaagc attatcgaac
      181 aataaagcga ggaacctctt acagtaagca gatccatatc actgaatatg tcctcttttc
      241 caaaggtatt tccaagatag taagaagata aaaaatcaaa agtatatcta tcaacattct
      301 tattaccaaa ctcaggataa tcaatatcta aaagatcagc atctataaga atatgtgata
      361 aacccatgga aacagcatag gcataattgc ctttctgctc cacacgaaaa gaaaaaggtg
      421 caagtctcga ctctgcaaca agaaaagcaa gtatgagctc tggaggaaca tcataaacaa
      481 taaccttctg cttaaaaaaa ctttccatag gaacaacggg actgtaaaaa gttaaaaaac
      541 tattactgta tgcatatgca acatccacga gatattttga ataagcgttt atatcctgct
      601 tggtaaattc tttattcgga aaaatataag caaaccaact cttaaaagaa gaggcactta
      661 actcaaaata ataatccata agcttattca ttctgaaaac caaatcggta cgttcaggat
      721 aatcatctct aagaataggg ttatacttaa gcttatacaa ctctctgtaa taagtattat
      781 ctacaggaac catctcgtca aaataatcaa gcatgccact ggatttgcag tttgaacggg
      841 catcagtaaa agattcaaat atcctgctaa catattcttt cacatcatcc ctgtaattta
      901 aaacttctgg ttgcttttcc acaggtttgg tttctacagg ctttgaaacg gctgtaggag
      961 tagctacagc tgtaaccgta cttacactga cataagtttt cagatcttta acctgaacaa
     1021 gattaagttc acctataaag tacttatagt atgagagaac aaagtcatcg taggtataac
     1081 ctgccccttc cgaaattgat tttaccgatt caaaagaaag attataacct ttattataca
     1141 ttacggccat gtatcgtatg aaattatccc tttcagtcgg atgtgaaaat atagccctgg
     1201 gatctctgaa acttatcacc tgattttccg catcatttga aaaaacaagg gtgaaggatg
     1261 ccaaaaatat agctgcaaaa aaccaaaact ttttcaaaaa acacctccga acagattaaa
     1321 aactatcttt ataagtgata agcttctggt aaagcaactg catgaatatc gaagaataaa
     1381 gcagtgatcc ccttacggtt aacaggtcgt agtcactgaa gtacgtcttc tgcttagctg
     1441 tattgccaag ataacggctg gatataatat caaagttacg tccacctgca tcattatttc
     1501 cgatatcgtc atgagccgct gctatttcaa gaaaatcggc atcgataaga gtatgtgcaa
     1561 gtccgaatga aacagcatat atatttttgt tggatgaaat ttccgctctg aaggatcccg
     1621 gaagaagcct tgattcctga acaaaaaaag caagaaccat ttcgaccggt atatccttta
     1681 taattacgct tgaacggaaa aaagaattgt ctatactgct tgtaaaggaa aggttcgaac
     1741 ctatatacgc ataagaaaga tccacaagca tttctgaata cttttgggta tcgaaggtat
     1801 caacagattc ggtaaatacc tttgcaaaat gtggtttgaa aaattcagaa cttgttttat
     1861 agtagtcaag aagatccttt actctggtca caaggaaggt acgatttgaa aaaggaatta
     1921 aaggcggatt aaaattattt ttgtggtcag ttctgtattc gtttgccttt acaggaacct
     1981 catcgtcaaa tttattgaaa ataccgtcac tgttttggtt tgacagagaa cctgaaagtt
     2041 ttgaataaat ctcttttatg taatcagcta ccctggaatc atacttgtct tctgttctgt
     2101 cttcagaacg tgtttttata ctgtctaagg tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn naatcatact tgtcttctgt tctgtcttca gaacgtgttt ttatactgtc
     2281 taaggttacc tctccattta ggtacttata aaaagccagt acgttgtcgt cggctgtata
     2341 agaagggtta tcggcaaatc ctttaatctg ttcgaaggta tagttgtaac ctttattgta
     2401 tgctgtataa gcatatctga caagcttctc ttcctgggtt ttggtaagac ccttaaaatt
     2461 ttcttcttca ggttttttta taacgtttat taacatatcg catgaagaaa aaaatagagc
     2521 ggtggagata agtaacagca gaaaaaaagt tttttttcat tttgcccacg tccctttcta
     2581 atatttttaa aacaatgatt ttccgatggt cattataatt atagcaaatt taataaaccc
     2641 ttcaaagttt taaattaata tatagtataa ggggaaaatg aaggtgatgg aaaaaatgaa
     2701 taaaaatcat atacccataa tgattgttct ggtttttatg tgtcaattcc tttatgccgg
     2761 tgacgttaag tatgtatata cacctgcagg tcccgagggg atgattatat atgatgcttc
     2821 agatatctca cagatgaagg ttgtgagtac attcaacctt ggaggatatg taaaagatct
     2881 tgtgccggga gaaaaggttg tatacgctat tgcggataac gtcggagtta tctgcattga
     2941 tatttctaat gttcttaaac ccaggattat aggaaaattg aattttgccg atgttcttag
     3001 aaaaatagag atttcaacag aaaaagctta catagactat tatgacagta ttcttgataa
     3061 aagcggaatt gcagttatag acataaaaga ccctaggaac atgaagtttt tatatgaaat
     3121 atttgaagag gactatgttt cctcttatgt ggtaaacggt gattacatat atctttcaga
     3181 tggacgtgaa ggtctcaagg taattcaagc atacgaagat acatataaat tggtcagccg
     3241 ttataaaatt aattcttcaa agctttttta caataataat atgctttttg ccggggaaat
     3301 aaacagcgct atatatatct ttgacgtgac tgatcctaaa aagctgcttt tcaaaagcaa
     3361 gtacagtttt ccaagggatt tttcggagct tgccgtggat ggtgacagac ttttcgttct
     3421 ttgcaaggag caaggattat atatgttcaa ggtaaattat gaaagtggag taaactatct
     3481 tacaaagtta tactccgatg atataaaaac tttccacatc agcggaaata tgttgttgct
     3541 gcttataaat tcagatttgg accgtgaaga tggacttgtt gtgctggata tttcagatat
     3601 ttattcacca tcaataataa aacaggattt ttcatttata aacggagagg caaaagatat
     3661 cgttgtatac ggtaaatacg cttatatagc tgattctaat aaaggaatca aaatatacga
     3721 tgtatcaaac gttaaaaaat cgaagtatgt aaaaacaatt gattgcacag gcagctttga
     3781 ggcacttttt cttgaaggga agaggcttta tgctatgctt gacagatccg gaataagtat
     3841 atacgatctg tccgacccgc ttactccaaa aaaacttgga aatgttgctg aagcaacatt
     3901 gaaaaatagt atttatttaa aagcagacat gttgtttatg tcacaaggca atactgtaaa
     3961 ggtatacaat gtatccgacc ctgaaaacat aaaacagatt tccagcatta ctgatataag
     4021 tgaagtgaat aacggagaga tcataaaaaa tataaggata agcgacaagt atcttatact
     4081 ttcaggttac agaaccaact caggcggaat aaagtcaggc ttcataaaaa tatttgatat
     4141 atcagaagta gaaaaacctt tatgtattaa aagatttcag gatataggaa taggatgggt
     4201 aagtcccgta agctctccat acttcgatcg ctattatcta tatatgggac ttacagatat
     4261 acaaggttat gaacggatga aaacctttac tgttttggat atttttgata tgagaactat
     4321 gaaggttata ggcagcgctg attatgaaga taatatagga aaaaagtaca ctataggagt
     4381 tcaggatctt gaaatataca acaactatgc cttgctggcc tgtggtgaag acggaataaa
     4441 aatattcaat ataagggatc caaaaaagcc attttatgtt acttctgtaa aaacaggagg
     4501 atttgcctca agtatatttg tgaaatcgaa ttacgcatat gtggcagacg gagaaaacgg
     4561 ttttttcatt ctggatataa gaaatcccaa taatccaata atttcatacg atgctttatg
     4621 gataaacatg aagaagataa aaagctgaga gaggttaacc tctctcagct tttttaatac
     4681 tttccaaccg gagccatgta tattacaaat tcttcttttc cgtcagcttt tataaaatta
     4741 tccataagct tttgagagta ggcgcctatg gcacatgttc cgcaggttat attttcacat
     4801 gccagataaa gattctgaca gacgtgtccg gcatccagaa gaagcagttt gtaagagctt
     4861 tcggcatatc tccattccat tctgtatgga ataacgctcc agataaaagt aactgcagat
     4921 tcgcctgcca tatgctgacc atagcaacct tcgattattt tttgcgaaag atcaggctga
     4981 gtataagaaa gaaacagctt atgctctatc ggaagatatc tgtaaacacc ttcttcaaag
     5041 ccttcaacat ttctaatata aaggtatgtt tcaaaagcat gccttgcgcc tgccgaagga
     5101 actgttctca aagatgcgta tccgtttctt atgatctctt ttataccctg agtactccac
     5161 agaaggaatg aaagctcatt taaagacaaa ggagtgttct taaaagctct tctgcttttt
     5221 ctgtttttca gcagctcagt cagtgaaaga ctcggaacct tgaactcttg cggagaaaca
     5281 aggtcaaaga gcctgctttc ctgcggataa ggcttttcca aaggcggctg aggtatcttt
     5341 ttttgctggt ctgtttcctc gtcatattca tcaaaaatcc cgttgctgac aaatttacgc
     5401 agttgttcga tattaaaccg gataaaaatc cctcccttaa aataatatat attttcccat
     5461 aaaaaattta tacagataaa agttccgcta catgtaaatt atagcaaaag tcaagttata
     5521 atatcactat aaaaaccata aaatataccc ggaggaaaac ataaatgaaa acaccagata
     5581 gaaaaaccgc tcacgatata ctcaatcagg cttcagaaat gaatccggga aagtggatcg
     5641 agcattcaaa aaacacagcc tatgccgctg agaaaatagc ccgacacata aaaggattag
     5701 atccggaaaa agcatataca gcaggtcttt tgcatgatat aggaagacgc gagggagtta
     5761 ggaatcttca tcacataatc tgcgggtatg agtatatgat gtcacttgaa tacgatggaa
     5821 cagccaaaat atctatgacg cattcctttg ctcttaaaaa tttgaacact tatcttggag
     5881 agtacgactg tactgatgct gagcttgatt ttatacgtac ttatattgaa aatgtacagt
     5941 acgacgatta cgatcttttg atacagcttt gcgactgtct tgcacttgcg gacgggcttt
     6001 gcataattga acagagactc gttgatgtag ccatgagata tggatttaat gaacttacta
     6061 ttccaaaatg gaagaagata tttgaaataa aaacatattt tgagaaaata atgggcacaa
     6121 gcatatacga tgttttgggt ataatgcccc gtcagatgaa ataaatctta atatatgtta
     6181 aagttataaa aaaaagaata gtatataata t
//
LOCUS       DAOSHR010000122         6188 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_11152_length_6188_cov_6.282896, whole genome shotgun sequence.
ACCESSION   DAOSHR010000122 DAOSHR010000000
VERSION     DAOSHR010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 6188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6188
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11152_length_6188_cov_6.282896"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..1936)
                     /locus_tag="PLU28_07415"
     CDS             complement(5..1936)
                     /locus_tag="PLU28_07415"
                     /inference="COORDINATES: protein motif:HMM:NF020660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1926 domain-containing protein"
                     /protein_id="HOG34928.1"
                     /translation="MKYLLFGTHNHQPHGNFTHVLEYAYEKSYKPFIETLSRCPDFKA
                     VIHFTGELLDWIEQKDNSFIEKLSLLIKHNQVEILSGGFFEPILPCIPSCDRISQLSM
                     LNNRIKELFGISPKGAWIAERVWNSEIAADLVKCGIKYVFLDDFQFEYSGCNSSSIVK
                     IQSQQDAQLAVIPISQPLRYSILNEPPKKVTELIENFPGNFVVFMDDGERFGMWGSSY
                     TYCYSEKYLMNLFNMISENPNIKPVLANELVELLEGSDGEIQPSSYPEMMIWSMDTDL
                     RTRFENSLVDMKTLKSNSVEWTNFIKKYSIVNVLYSKMLMISHEYDSNYLHGKDEYRS
                     LLAAQANDAYWHGMFGGCYLPNLRDALFSAVIKAESALSDSSRMISSYRGKFFYVKYS
                     EISVMADPDDGGKIVDLSYMPSCFCLSNVLTRQEEAYHKKMKQINPDLNNKIVLDWYN
                     KGLFIDHFPASEKDAHSWQTNDTREKGDFANQPYVLTCAEKSFTLERTGHVWVNSDWP
                     EVKIRKGFCISKNQLCADYSISSEYEFETDFSVEINMNFLAQDSPDRFITINEENFPV
                     NLKGKFSTDNLLITDLYKKVKVRLQGSSDNVILFPLYTFSCSDLGNDTIYQGSSIVFT
                     KKIKISSKVTNLSYSIDFL"
     gene            2009..2671
                     /locus_tag="PLU28_07420"
     CDS             2009..2671
                     /locus_tag="PLU28_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34929.1"
                     /translation="MAEKKPKKKRSLKTVIIFFFVGIIIAVFLLTFGYIGFEFNRLSY
                     MNMSLANDWRSYLSYITGKIPFLNNFIKYKYLEIGDPLYVQNEIVSMRLQAIKEQSDE
                     VTVKNSDLQKMMIDISAQASSLAVQKQELERKSTELSQRENILADYETRVQKLASWFG
                     EATPQQIAVALSRDEVGIDLLVDALYRLRSDSASDILTALSNVNPQKAAQVIAKLGEK
                     QR"
     gene            2682..4568
                     /locus_tag="PLU28_07425"
     CDS             2682..4568
                     /locus_tag="PLU28_07425"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HOG34930.1"
                     /translation="MSEEILLGNLLNRLKLNVEVKTSEKADLQTDNKEFEKVMQKFQD
                     YSSSAKEREVSENKDDNKNKHDALETIKKNELTKTEEKYVSQKVIPDTVTKESQEVIE
                     NEEDGIQKILKKLINQLLTLNNKKSDSEKEKEKVNKVCVLITQLINNGNTENKAQQNQ
                     VFKQLVQKAAEIGIKVKDVKKGSLGMLLVTDKNMNQSVVIPKVTIKKAVAVSKESEVK
                     NKDTQQSKVSLKNKTVAAEKKSEDISLVQTKSASSEKTSRREIKEAQMETKKATVIIP
                     RDSETVKQESPKISIKAENINKILSFASQALKNNETKKTENFSQVLFNASINKNFERR
                     QIFTENKNIGEKNWVPLRTPEKTNDFFNIKSEMPFIPLSDMKKESSFILQKKESIKKD
                     EMAVERVTEKDKEAPQKIQSIKEQPENEKPTEKRSELTQRQTNNEDIHKEITVEKTFE
                     DVYEHMQTQTAQTDSKSGSTIISDSTSTARKSVALGPETIRNTSVQNVPLERLNTDLH
                     RIIEQKLTQRVFTESMDLQITPPNLGKVNLELIKTGMAISINVITETDSSRESISRNL
                     QSLVGTLRNEGYNPVQVNIKSEHKNDLMQDNQQKQPNYKDQQEQKKDDSQEFEKILRG
                     EENV"
     gene            4561..5229
                     /locus_tag="PLU28_07430"
     CDS             4561..5229
                     /locus_tag="PLU28_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HOG34931.1"
                     /translation="MFNGIEKNNLYLSTSEASRNKTIKKELDKDAFLQLLVTQLKYQD
                     PTSPLDNKELIAQMSQLSTTEQIMNMSKSFQGMVDSQVSLQKMQASSLIGKSAVVEAN
                     TVQLTQGVSENFTYNLEKDSVISIEIQNADGKTVYSENLGTVKKGVSTYLWNGRDNSG
                     TNMPDGEYEVKLYSYENGKKTEVGGLRGGKVEAVQFIDKEFYVLIDGKRYPVKSIKEI
                     SNIA"
     gene            5246..>6188
                     /locus_tag="PLU28_07435"
     CDS             5246..>6188
                     /locus_tag="PLU28_07435"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HOG34932.1"
                     /translation="MMRAMYSGITGLKNFQLQMDVVGNNIANVNTTGFKASRVTFETT
                     LLQTLKAGKSPENQYGGTNPMQAGLGSKISSVDKIFSQGSFQNTGKKTDLAIQGDGFF
                     VLSDSQGNYFSRAGNFTLDSSGYLVNPSSGLKLQGWSAKIGTNGQRYVDTNEPIGDVK
                     IAAGLVMPAKQTSFVNLAHNLNADVGVQETTMVVKSTLGKELPIKFKFVRDMRAEYKE
                     RNVYTWESELVNTGSPAYEFAKSNYDKTLKGSVELDNAGNVVKWSTFKNGMETMGVDT
                     DSKLSIYDVSGNLRINRSNEAVDGSSAGMEIDFSTVTY"
BASE COUNT         2199 a         1022 c         1281 g         1686 t
ORIGIN      
        1 tggttcagag aaaatctata gagtaactga gatttgttac tttagaagat atttttattt
       61 tcttagtgaa cacaatgctt gacccctgat atattgtgtc attcccaaga tcggagcatg
      121 aaaaagtata taaaggaaaa agtattacat tgtcagaact accttgaagc cgtactttta
      181 cttttttgta aagatctgtg atcaaaagat tatctgtaga gaactttccc ttgaggttta
      241 caggaaagtt ctcttcgttt attgtaataa atctgtccgg tgagtcctga gccagaaagt
      301 tcatgtttat ttccacagaa aaatctgttt caaactcgta ttccgatgaa attgaataat
      361 ctgcacaaag ttggtttttt gatatacaaa aaccctttct tatttttact tccggccaat
      421 cgctgtttac ccatacatgc cctgttcttt caagagtaaa agacttttct gcgcaagtaa
      481 gtacataagg ctgattagca aaatcgccct tttctctggt atcatttgtc tgccagctgt
      541 gagcatcttt ttctgaagca ggaaaatgat cgataaaaag tcccttgtta taccaatcaa
      601 gtactatctt gttgttcaga tctgggttaa tctgtttcat ctttttatgg taggcttctt
      661 cctgtcttgt gagaacattg ctgaggcaaa aacaggaggg catataagac agatctacaa
      721 tctttccgcc atcatcaggg tctgccatga cagatatttc ggaatatttt acataaaaga
      781 actttccgcg gtatgagctg atcattctgg aggaatcgct taaagcagat tctgctttta
      841 taacagctga aaaaagcgca tctctgagat ttggcagata gcagcctccg aacattccgt
      901 gccagtaagc atcgtttgcc tgagccgcca gaagagatcg gtattcatct ttgccgtgaa
      961 gatagttact gtcatattca tgagaaatca taagcatttt tgaataaaga acattaacta
     1021 tggagtattt ttttatgaaa ttggtccatt ccacactatt tgatttcaag gttttcatat
     1081 ccacaagaga attttcaaaa cgggttctta aatctgtatc cattgaccaa atcatcattt
     1141 ccggatatga agagggttga atttcaccat ctgaaccctc aagcaactct acaagttcat
     1201 tggcaagaac aggctttata ttagggttct ctgaaatcat attaaaaaga ttcataagat
     1261 atttttcaga gtaacagtac gtatagcttg atccccacat accgaaacgt tctccgtcgt
     1321 ccatgaaaac tacaaaattt ccagggaagt tttctataag ttctgttact ttttttggtg
     1381 gttcattcaa gatactgtaa cgaaggggct gacttatagg tataactgct aactgggcat
     1441 cctgctgtga ctgtattttt actattgagg aagagttgca tcctgaatac tcaaattgga
     1501 agtcgtcgag aaagacgtat tttataccgc attttacaag gtcggccgca atttcagagt
     1561 tccatactct ttcggcaatc catgcccctt tgggtgagat tccaaagagc tctttaattc
     1621 tgttattaag catactaagt tgggaaatac ggtcacaact tggtatacat ggtaagatag
     1681 gttcaaaaaa tcctcccgag agtatctcaa cctggttgtg ttttataagc aaggaaagtt
     1741 tttctataaa agagttatcc ttttgttcaa tccagtcaag cagttcgccg gtaaaatgta
     1801 tgaccgcttt aaagtcgggg catctggaca atgtttctat aaaaggcttg taactttttt
     1861 cgtaagcgta ttcaagcaca tgggtaaaat ttccatgcgg ctggtggtta tgagttccaa
     1921 agagcaagta cttcatcagg gggacacctc aactttttta ggaatagttt ataatcaaat
     1981 agaaatataa aatgtgagga ggtacagtat ggctgaaaaa aaacccaaga aaaaaagaag
     2041 tttaaaaact gttattatat ttttctttgt cggtattatt atagcagtat ttctattaac
     2101 ttttggctac ataggatttg aatttaacag actttcatat atgaatatga gtctggctaa
     2161 cgattggagg agttaccttt catatatcac tggaaagata ccttttctga ataattttat
     2221 taaatacaaa tatcttgaaa ttggagatcc tctttacgta cagaatgaaa ttgtgagtat
     2281 gaggctgcag gcgataaaag aacagtcgga tgaagttaca gtcaaaaact ccgatcttca
     2341 gaagatgatg atagatattt ctgcacaagc aagttcactt gccgtacaga aacaggaatt
     2401 ggaaagaaag agcaccgaat taagtcagag ggagaatata ctggcagatt atgaaacaag
     2461 agtgcagaag cttgcctcgt ggtttggaga agcaactcct cagcaaatag cagtggctct
     2521 ttccagagat gaggtgggaa ttgatctgct ggttgatgct ttgtaccgtc taagatcaga
     2581 ttcggcatct gatattctta cggctctgtc taatgtaaat ccgcagaagg cagcacaggt
     2641 catagcaaag ttaggtgaaa aacaaaggtg aggtgagtga attgagcgaa gagattttac
     2701 tcggaaacct tctgaacaga ctgaagctga atgttgaggt taaaacttct gaaaaggctg
     2761 accttcagac agataacaaa gagtttgaaa aagtaatgca gaagtttcag gactattcat
     2821 cgtcagcaaa agaaagagaa gtttcagaga acaaggatga taataaaaat aagcatgatg
     2881 ctttggaaac gataaaaaag aacgagttaa ccaaaaccga agaaaagtat gtgagtcaaa
     2941 aagttatacc ggataccgtt acgaaagaat cacaggaagt gattgaaaat gaagaagacg
     3001 gtattcagaa gatacttaaa aaacttataa accagcttct gactcttaat aacaaaaaga
     3061 gtgacagtga gaaagaaaaa gagaaggtaa acaaggtatg cgtgctcata acacagctga
     3121 taaataatgg aaatacggaa aacaaggctc agcaaaacca ggtttttaaa cagcttgttc
     3181 agaaggctgc agagattgga ataaaggtaa aggatgttaa aaagggttct ctgggtatgc
     3241 ttttggtgac agataaaaac atgaatcagt cggttgtgat tcctaaagta actatcaaaa
     3301 aagcagttgc agtaagcaaa gagagtgaag tcaaaaacaa agatacacag cagtcaaaag
     3361 tttcattaaa aaataaaacc gtagctgcag agaagaaatc cgaagatatc tccttggttc
     3421 aaaccaaaag cgcgagttca gaaaaaacaa gcagaagaga gatcaaggaa gcccagatgg
     3481 aaacaaaaaa agcaacggta attattccaa gagattcgga aacagtcaag caggaaagtc
     3541 caaagatatc tattaaagca gaaaatataa ataagatact ttcttttgcc agtcaggctc
     3601 taaaaaataa tgaaaccaag aaaacggaaa atttttcgca ggtacttttt aatgcaagta
     3661 ttaataagaa ctttgaaaga agacagattt ttacagaaaa caaaaacatt ggtgaaaaga
     3721 actgggttcc cttaagaacg ccggagaaaa cgaatgattt ttttaacatt aaatcagaga
     3781 tgccgtttat accgctttct gatatgaaaa aggaaagttc ttttatattg cagaaaaaag
     3841 aaagcattaa aaaagatgag atggcagttg agagagtaac tgaaaaagat aaagaagcac
     3901 cacaaaagat acagagtatc aaagaacaac ctgaaaatga aaagcctaca gaaaaaagaa
     3961 gtgagttgac acaaagacaa accaataatg aagatattca taaggaaata accgttgaga
     4021 agacttttga agatgtttat gaacatatgc agacacagac tgcacagaca gacagcaaat
     4081 ccgggagcac aattatctca gacagcacat caacagcaag aaaatcggta gctttgggtc
     4141 cggaaaccat tagaaacact tctgttcaga atgttccgct cgaaagactt aatacagatc
     4201 ttcacagaat aattgaacag aagcttactc aaagggtttt cacagaaagt atggatcttc
     4261 agataactcc tcccaatctg ggaaaggtaa atctggaact tataaaaacc ggtatggcca
     4321 taagcattaa tgtcattact gaaactgata gctcaagaga aagtatttcc agaaatcttc
     4381 aaagtcttgt aggaacatta aggaacgaag gatataatcc cgttcaggtt aatataaaat
     4441 cagaacataa aaatgatctt atgcaggata atcagcaaaa acaacctaac tataaagacc
     4501 agcaggaaca gaaaaaagat gattctcagg agtttgaaaa gatattgaga ggtgaggaaa
     4561 atgtttaatg gaatagaaaa aaataattta taccttagta caagtgaggc ttcaagaaac
     4621 aagactataa aaaaagagtt ggataaggat gcttttttac agcttctcgt aacccagtta
     4681 aagtatcagg atccgacttc tcctcttgac aacaaggaac tgattgcaca gatgtcgcag
     4741 ctctctacaa ctgaacagat aatgaatatg agcaagtcat ttcagggtat ggttgattca
     4801 caggtttctc ttcagaagat gcaggcttcc tcacttatag gaaaaagtgc ggtggttgaa
     4861 gccaatacag ttcagctcac acagggagta tcagaaaatt ttacttacaa tcttgaaaag
     4921 gattcagtaa taagtataga gatacagaac gctgacggca aaacagttta ttctgaaaat
     4981 ttgggaacgg ttaaaaaagg cgttagcaca tatctttgga acggaaggga taattccggt
     5041 acaaatatgc ctgacggtga gtacgaagta aagctatata gctatgaaaa cggtaagaaa
     5101 accgaagtag gtggtcttag aggtggtaag gttgaagctg tacagtttat tgataaggaa
     5161 ttttatgttc tcatagacgg aaaaagatat cctgtgaaaa gtataaaaga aatttcaaac
     5221 atagcataac gggaggaata atactatgat gagagcaatg tattccggaa ttaccggtct
     5281 taaaaacttt cagcttcaga tggatgtagt cgggaataat atagccaacg ttaatactac
     5341 aggcttcaag gcttcaagag taacttttga aacaacactc ctgcagactc ttaaagcagg
     5401 aaaatcaccg gaaaaccagt atggaggaac taaccctatg caggctggac tgggatcaaa
     5461 aatttcatca gttgataaga tattcagcca gggttctttt caaaacacag gtaaaaaaac
     5521 agatcttgcc atacagggag atgggttttt tgttctttcc gactcacagg gaaattactt
     5581 cagcagagca ggtaacttta cgctggactc ttccggatat cttgtgaatc cttcgtctgg
     5641 gttgaaactt caaggatgga gtgcaaaaat aggcaccaac ggtcagagat atgttgatac
     5701 caatgaacct ataggtgatg taaaaatagc agcgggactt gttatgccgg caaaacagac
     5761 ttcctttgtc aatcttgctc acaatcttaa tgcagatgtc ggagttcaag aaactactat
     5821 ggtagtaaaa agtacacttg gaaaggaact tcccattaag ttcaaatttg tcagggatat
     5881 gcgggccgag tacaaggaga ggaatgttta tacatgggaa agcgaacttg taaatacagg
     5941 ttctccggca tacgaatttg ccaaatcaaa ttacgataaa acccttaaag gttccgtgga
     6001 acttgacaat gcgggtaatg ttgttaaatg gagtacattt aagaatggaa tggaaacaat
     6061 gggtgtagat acagattcca agctttcgat atatgatgta tcaggaaatc ttagaataaa
     6121 cagaagcaat gaagcggttg acggttcatc ggccggtatg gagatagatt tttccactgt
     6181 tacttatg
//
LOCUS       DAOSHR010000123         6169 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_11193_length_6169_cov_5.988224, whole genome shotgun sequence.
ACCESSION   DAOSHR010000123 DAOSHR010000000
VERSION     DAOSHR010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 6169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6169
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11193_length_6169_cov_5.988224"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..726)
                     /locus_tag="PLU28_07440"
     CDS             complement(<1..726)
                     /locus_tag="PLU28_07440"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34933.1"
                     /translation="MKIVYDPRHVFYSPKSEFDRTRMVNNPETPQRIEEIIKVLKFKY
                     GNSFVKPKDFPKSYIYLVHDSNYISWLKNRCDSLEDMQEYFPKVFGYDRVFDNGTPLM
                     KNSFEMAWMGVKCALTGAYELLNGGEDIVYVGTRPPGHHAGIDMGGGYCYMNNSAVAA
                     KFLQMKSGGTVAVLDLDFHHGNGTQEIFYEDSSVLYISLHADPAQHFPFISGYEWEIG
                     GGEGKGYNINFPLAAGTDGRTYLR"
     gene            complement(730..1167)
                     /gene="rpiB"
                     /locus_tag="PLU28_07445"
     CDS             complement(730..1167)
                     /gene="rpiB"
                     /locus_tag="PLU28_07445"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOG34934.1"
                     /translation="MKVAIGSDHAGYDMKENIKKYLISQNVEVVDCGTHSDSSVDYPD
                     YAKAVCTNVSDKETALGILVCGTGIGMSIAANKIHGIRAALCLYPTMAELSRKHNNAN
                     VLVLGGRIMGTDIARWTVDAFIRTSFEGGRHQNRLDKIERMEE"
     gene            complement(1182..1475)
                     /locus_tag="PLU28_07450"
     CDS             complement(1182..1475)
                     /locus_tag="PLU28_07450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOG34935.1"
                     /translation="MEIITKTVEDRSVVVLDGELEMNNVSEVKKQILGFDFKEMIIDF
                     EKVTYLDSAGIGMLISLHKTALLKGGRLEIINVDSKIKKLFEMVGLTKILNIS"
     gene            complement(1489..2103)
                     /gene="lexA"
                     /locus_tag="PLU28_07455"
     CDS             complement(1489..2103)
                     /gene="lexA"
                     /locus_tag="PLU28_07455"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HOG34936.1"
                     /translation="MKTLTKRQEQILEFIKSYVSKNGYPPTIRDINQNFSMKSPRAAH
                     KHLLVLEKKGYIERNGVSRGIKLTAKSGDIFTKEKLVPIVGKVAAGEAIEAIENVTDF
                     IPLPANFFTESADYFGLRVEGTSMIEAHIVTDDYVIIKKQDSASDGDIVVALIDDNYA
                     TLKRFKGGTDMIHLIPENKNMDIIEVEPQRLKIQGKMVGLIRKL"
     gene            complement(2106..4604)
                     /gene="gyrA"
                     /locus_tag="PLU28_07460"
     CDS             complement(2106..4604)
                     /gene="gyrA"
                     /locus_tag="PLU28_07460"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOG34937.1"
                     /translation="MSENEMNEKNNIDPQHFENITEKRFVDEMKNSYLLYSMSVLISR
                     AIPDARDGFKPVQRRILYGMNELGLKHNTSYKKCARIVGEVMGKYHPHGDGSIYDALV
                     RMAQDFSMRYMLVDGQGNFGSIDRDPAAAMRYTEAKMQSMSEYMLNDLDKDTVNFVDN
                     FDGSLREPEVLPTKLPNLLMNGASGIAVGMATNIPPHNLGELVKGFKYLIENPDCQTS
                     ELMQFIKGPDFPTGGIIVDAENILSMYETGRGKFTVRGRYEIAEDKSGQAIVVTEIPY
                     NVSKVDIIEQIVAYAERMRDNKQDSGIRDIRDESDRHGMRLVIELKRNANVNKLTNDL
                     LKHTSLQTTFAVQMNVVYNRKPVMMTLKEIMNTFVEHRVDVVTRRTQYELNKARKRAH
                     IVEGLIKAVQGIDTVIDVIRNSESDEAIISNLMSIIGVTEEQAKAIADLKLKSLSKLE
                     INKFRQELEQLNIEISRCIKVLQDRSSLMQIIESEFDEVDQKFGDERKTEIIYGKSEL
                     KSPEELIEDQEVVVVLTQWGYIKSINSVEYKLQGRGGKGAKAIKIGDNDTILQVIHTR
                     RLSKLMFITSAGKAYQIPTYEIDMSSKDTKGVHIANYLSMQEGEKIKTIVSISLDGDY
                     DKDVMIFTKKGKVKKTSLREFANSKRSGVRAVSLNEDDAVVDSIIVEKEEGKILTVTK
                     HGMAICFNISDVRLMGRTAIGVNAMKIRKDDEIVNAVLIEDSKKLLIMTQNGYAKRES
                     FDEYRIQIRGGIGVKTLKNIKKIGPIVSAIAVSDDNHLLAFTTNGKAIRFSVDSIPVL
                     SRVTQGVIALRLDENDTTMGLAIVKEEEELLKEE"
     gene            complement(4632..>6169)
                     /gene="metG"
                     /locus_tag="PLU28_07465"
     CDS             complement(4632..>6169)
                     /gene="metG"
                     /locus_tag="PLU28_07465"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOG34938.1"
                     /translation="CPQCKREVKWVEEENYFFRLSKYNDKLLKYYEEHPEFMEPDFRR
                     NEMLQIIRSGLKDLSISRTTFDWGIPMPDDPKHVVYVWVDALINYLSALGYAENDQKF
                     HNYWPADLHLIGKEINRFHSIIWPAMLMSAGLELPTKVFAHGWLTVNGEKISKSLGNA
                     ISPIELMDVYGRDPVRYYLLRDINFGRDGDFSEDNLISRFNADLVNDLSNLVHRTLSM
                     CEKYFDGVIPVFSGKDDSPDKDLYSIIQSNVKKYKDNMDHYQFTQALESIWEIIRYAN
                     KYIDLTEPWKLDKQDNGRERLSKVIYNLIDSIRIVSVLISPVMPDTSEQILKKIGYGQ
                     DHLAEENFELHRLSSNVKTDKGNPVFMRIDTAQWEKVIKIKSPDIKQNSFAPSIKKDE
                     TVNQEKKDNIISLIEIEDFKKVDLRVALVKSAENIIKSEKLLKLSLDMGELGIRQVVA
                     GIAKFYTPQEITGKKIVVLSNLKPTKLMGIESCGMLLAAKINDKLSVLIADRDLEPGA
                     KIS"
BASE COUNT         1722 a         1408 c          910 g         2129 t
ORIGIN      
        1 tctaagataa gttcttccat cagttccggc agcaagggga aaatttatat tataaccttt
       61 cccttcaccc ccacctatct cccattcgta tccacttatg aaaggaaaat gctgtgcagg
      121 atccgcatgg agactgatat aaaggacaga actgtcttca taaaagattt cctgagttcc
      181 gtttccatga tgaaaatcaa ggtcaagcac cgcaacagta cctccagatt tcatctgaag
      241 aaactttgca gcaacagctg aattgttcat atagcagtaa ccgccaccca tatctatgcc
      301 ggcatgatgg ccaggaggtc ttgtcccgac ataaacaata tcctctccac cattaagaag
      361 ctcataagct cctgtaagag cacattttac acccatccaa gccatttcga aagaattttt
      421 catcaaagga gttccgttat caaaaaccct atcataacca aaaacctttg gaaagtactc
      481 ctgcatatct tcgagagaat cacatctgtt cttaagccaa cttatataat ttgaatcatg
      541 tacaagataa atgtaagact ttggaaaatc cttaggtttt acaaatgaat ttccatattt
      601 aaattttaaa acctttatta tttcttctat tctttggggt gtttcaggat tatttaccat
      661 cctggtcctg tcaaactcgg atttggggct gtaaaaaaca tgcctgggat cataaacgat
      721 cttcattttt tactcctcca tacgctctat tttatcgagc ctattctgat gtcttcctcc
      781 ttcaaaagaa gttcggataa aagcatcaac cgtccatctt gctatgtctg tacccattat
      841 gcgtccccct aaaacaagaa cattggcatt attgtgcttt cttgaaagtt ccgccattgt
      901 gggataaagg cataaagcag ctctgattcc atgaatctta ttggctgcta tagacattcc
      961 tatcccggtt ccacagacca agattcctaa agctgtttcc ttatccgaaa cgtttgtaca
     1021 gacagccttt gcatagtcag gataatcgac actgctgtca gaatgagttc cgcaatcaac
     1081 aacctcgaca ttttgcgata tcagatattt ttttatattc tctttcatat catatcctgc
     1141 atgatctgag cctatggcaa ctttcataaa gaaaaccctc cttaggatat attgagaatc
     1201 tttgtaaggc caaccatctc aaacaacttt tttattttgc tgtccacatt tataatctcc
     1261 agacggcctc cctttaacaa ggcagtttta tgcagactta taagcattcc tattccggcg
     1321 ctgtcaagat atgttacttt ttcaaaatca ataatcattt ccttaaaatc aaatcccaga
     1381 atctgttttt ttacttccga tacattattc atttcaagtt ctccatcaag aacaacaaca
     1441 cttctgtctt caaccgtctt agttatgatt tccatgataa cctccagttc agagctttcg
     1501 gatcagtcca accatcttcc cctgaatctt aagcctttgg ggttcaactt ctattatgtc
     1561 catatttttg ttttcaggaa taagatgaat catatcagta ccacctttaa atcttttcaa
     1621 agttgcatag ttatcgtcta tgagagctac aactatatcg ccatcagatg cagaatcctg
     1681 tttttttatt attacgtagt catcagtaac aatatgcgct tctatcatcg aggtaccctc
     1741 caccctcaga ccaaagtaat ccgctgattc tgtaaaaaaa tttgcaggta aaggtataaa
     1801 atcggtaaca ttctcaatcg cttctatcgc ttctcctgca gccactttac caactatagg
     1861 aaccagtttt tcttttgtaa atatatctcc tgatttagcc gttagtttta ttccgcgtga
     1921 cacaccgttt ctttctatat agcctttttt ttccagtacc aaaagatgct tatgagctgc
     1981 tctgggactc ttcattgaga aattctgatt tatatctctt atggtaggcg gataaccatt
     2041 ttttgaaaca tatgacttaa taaattccag gatttgttcc tgccttttag tcaatgtttt
     2101 caattctact cctcctttaa aagttcttct tcttctttta caatcgccaa acccatggta
     2161 gtatcgttct cgtcgagcct aagtgcaatt actccctgtg tcaccctgct caaaacaggt
     2221 attgaatcga cagaaaatct tatagcttta ccgttggtcg taaatgccaa cagatgatta
     2281 tcgtcagaaa cagctatcgc tgaaactata ggacctattt tctttatatt tttcagagtt
     2341 tttactccga taccgcccct tatctgtatt ctgtactcat caaagctctc tctcttggca
     2401 tatccgttct gagtcattat aagaagcttt ttgctgtcct ctatcaggac tgcattgaca
     2461 atttcgtcat cttttcttat tttcattgca ttgactccga ttgcagttct tcccatgagt
     2521 cttacatctg atatgttaaa acatatcgcc ataccgtgct ttgttacagt cagaattttt
     2581 ccttcctctt tttcaactat tatcgaatct acaactgcat catcttcatt aaggctaact
     2641 gctctcactc cagaacgttt ggaatttgca aactcccgca gagatgtttt tttcactttt
     2701 ccctttttgg taaatatcat cacatcttta tcataatctc cgtctaggga tatacttaca
     2761 atagtcttaa ttttctctcc ttcctgcatg gaaagatagt tagcaatatg aacacctttt
     2821 gtatctttcg aactcatatc tatctcatac gttggtattt ggtatgcctt gccggcagaa
     2881 gttataaaca tcagtttgga aagccttctg gtatgaataa cctggagtat ggtatcgtta
     2941 tcacctatct ttattgcttt tgcgccttta cctccccttc cttgcagctt atattcgact
     3001 gaatttatag actttatgta tccccactga gtcaggacaa ccacaacttc ttgatcttca
     3061 ataagttcct ctggactttt taattcggat tttccatata ttatctcagt ttttctttca
     3121 tctccaaact tttggtcaac ttcatcaaat tcactttcaa ttatctgcat aagagaactt
     3181 ctgtcttgaa gtacttttat gcaacgcgaa atctcaatat tcaactgctc aagctcctgc
     3241 ctgaacttat tgatttccaa ctttgacaaa ctcttgagtt taagatctgc aatagctttt
     3301 gcctgttctt ccgtcacacc tatgatactc ataagattac ttattatagc ttcatcactt
     3361 tcggaatttc ttatgacatc tatcacagta tctattccct gtaccgcttt tataagaccc
     3421 tctactatat gagccctttt tctggctttg ttaagctcat actgcgtcct acgtgtgact
     3481 acatcgactc tgtgctcaac aaaagtattc ataatctctt taagagtcat catcacaggt
     3541 tttctgttat acacaacatt catctgaaca gcaaaagtcg tctgcagaga tgtatgtttt
     3601 aggagatcgt tggtaagctt atttacattc gcatttcttt taagctcaat aacaagcctc
     3661 ataccatgcc tgtcagattc gtcgcgtata tcccttatac cagaatcctg cttattatct
     3721 ctcattcttt cagcataagc aactatctgt tctattatat caacttttga gacattatag
     3781 ggaatctcag taacaactat agcctgaccg ctcttatctt cggctatctc atatctgcct
     3841 ctaactgtaa acttccccct gcctgtctca tacatactta aaatgttttc cgcgtcaact
     3901 attattccgc cggtaggaaa atcaggtccc ttaataaact gcataagttc tgaagtttga
     3961 cagtcaggat tttcaataag atacttgaat cccttaacca attcacccag attatgaggc
     4021 ggtatattgg tagccattcc tacggcgatt ccacttgcac cgttcataag aagattcgga
     4081 agtttagttg gaagtacctc cggctccctt agagaaccat caaaattatc tacaaaattt
     4141 acagtatcct tatcaaggtc attgagcata tactctgaca tactctgcat tttagcctcg
     4201 gtatacctca tggctgctgc cggatcccga tctattgaac caaaatttcc ctgtccgtca
     4261 acaagcatat atctcattga aaagtcctgc gccatacgta caagggcgtc gtatatagaa
     4321 ccatctccat gcggatgata ctttcccatt acttcaccga caatacgggc acattttttg
     4381 tacgaagtat tgtgtttaag tccaagttca ttcataccat aaagaattct cctctgaaca
     4441 ggcttaaaac catctcttgc atcaggaatg gccctgctta tcaaaacact catcgaataa
     4501 agcaggtatg aattcttcat ttcatcgaca aaccttttct ctgttatatt ctcaaaatgc
     4561 tgcggatcaa tattgttttt ttcattcatt tcattttctg acacaaaaat tcctcctgta
     4621 caaaataaat tctatgatat ttttgctccc ggctctagat ctctatccgc aatcagcact
     4681 gaaagcttat cgtttatttt agctgccaga agcattccac aggactctat ccccataagc
     4741 ttcgtaggtt ttagatttga aagcaccaca attttttttc ctgtaatttc ctgcggagta
     4801 taaaactttg ctatcccggc tactacctgc cggattccga gttctcccat atccaatgac
     4861 agtttcaaaa gtttttctga ctttattata ttctcagcac ttttcacaag tgccactctc
     4921 aggtctacct ttttaaagtc ttctatttca atcagagaaa taatgttgtc tttcttttct
     4981 tggttaactg tctcatcttt cttaattgaa ggtgcaaagc tgttctgttt aatatctggt
     5041 gattttattt ttataacttt ttcccattgg gctgtatcaa ttctcataaa cacaggattc
     5101 cctttatctg ttttcacatt agaagaaagt ctatgcaatt caaaattttc ttctgcaagg
     5161 tgatcttggc catatccgat ttttttaaga atctgttcag atgtgtctgg cataacagga
     5221 cttattaaaa ctgaaactat ccttatcgaa tctataagat tgtaaataac tttggaaagt
     5281 ctttcgcgcc cattatcctg tttatccagt ttccatggtt cggtaagatc gatatacttg
     5341 tttgcgtatc ttattatttc ccatatcgat tcaagagcct gggtaaattg atagtgatcc
     5401 atgttatctt tatacttttt tacattactc tgtattattg agtaaagatc cttgtctgga
     5461 ctatcgtctt tgcctgaaaa cacaggtatt acaccatcaa agtacttttc acacatagaa
     5521 agggttctgt gtacaagatt actcaagtca ttgactaagt cagcattaaa acggcttatc
     5581 agattatcct ctgaaaaatc accgtctctt ccgaaattaa tatcccttaa aagatagtat
     5641 cttacaggat ctctaccata aacatccata agctcaatag gacttatggc attgccaaga
     5701 gatttagaga tcttttcacc atttaccgta agccatccat gagcaaaaac ctttgttgga
     5761 agctctaatc cggccgacat aagcattgca ggccatatta ttgaatgaaa tctgtttatt
     5821 tcttttccta taaggtgaag atctgcaggc cagtaattat gaaatttctg atcattttca
     5881 gcatatccaa gagcactcag ataatttata agagcatcaa cccagacata aacaacatgc
     5941 ttgggatcat ccggcatagg aattccccaa tcaaaagtgg ttctgcttat acttaagtcc
     6001 ttaagcccag aacgtattat ctgaagcatc tcattccttc tgaagtccgg ctccatgaac
     6061 tccggatgtt cttcataata cttaagaagt ttgtcattat actttgatag tctaaaaaaa
     6121 taattttctt cctcaaccca ttttacttct cttttacatt gaggacaaa
//
LOCUS       DAOSHR010000124         5955 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_11749_length_5955_cov_5.443559, whole genome shotgun sequence.
ACCESSION   DAOSHR010000124 DAOSHR010000000
VERSION     DAOSHR010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 5955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5955
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11749_length_5955_cov_5.443559"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1534)
                     /locus_tag="PLU28_07470"
     CDS             complement(<1..1534)
                     /locus_tag="PLU28_07470"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HOG34939.1"
                     /translation="MKFSEMKYVRPDFNSTKAKFEQLIKSFSQASSFQEQSGFMEKIN
                     DLRRSFMTMQVLVNVRATINTKDSFYEEEMNFFDENGPLFEELNNSFYKELVKSNFRK
                     ELEEKWGKQLFALADLSLKTFSPEIINDLQSENKLTSKYTKLLGSAKIMFEGKERNLS
                     QMTPFEQSPDRQTRKKAVQAKFKWFEEHLEEFDGIYDELVKVRDTIARKLGYKNFVQL
                     GYDRLGRTDYNPVMVANYREQVRRYIVPMASKIFSKQQDRIGIDHLFYYDESFTFEGG
                     NPKPIGTYDDLVKKAQKMYRELSRETGDFFDFMIENELFDLVAKEGKAPGGYCTFIPD
                     YQSPFIFSNFNGTSGDVDVLTHEMGHAFQVYSSKDFKIPEYGWPTLEACEIHSMSMEF
                     ITWPWWELFYTENAEKARYMHLASALTFIPYGVSVDEFQHFVYENPQATPMQRREKWS
                     EIEKKYLPHRDYDAIGYLVNGGFWQRQAHIYKDPFYYIDYTLAQVCAFQFWSKFRQDR
                     EKA"
     gene            complement(1644..1718)
                     /locus_tag="PLU28_07475"
     tRNA            complement(1644..1718)
                     /locus_tag="PLU28_07475"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1684..1686),aa:Asn,seq:gtt)
     gene            complement(1813..3297)
                     /locus_tag="PLU28_07480"
     CDS             complement(1813..3297)
                     /locus_tag="PLU28_07480"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOG34940.1"
                     /translation="MIKDNKSSFSSKINKKITSTFIIYTLILLFIVHIVAAAVIKSYL
                     HENTLPPESMNFFILHLTAGIVFFSLVFSGFLFFLRHQIKKDISQPIDYILSYLQKIK
                     TGKKPSVEYQNNDEFKTILEEFEQLYENFNRSIQALQDSQERIKNSAGFKTNILKILS
                     HEIKTPLQSIIGFSEHLKSKLSDKMQLQMLGVMIDSSKELLAKFDRLIERSYLEENSI
                     NKFMLLEDFNPGNLVLEISSKYENLCFKKGLSFHFIHAPFTYDEKTIFVDSKKLRRIL
                     EELLDNAVKFTDTGDIWFEFNAQESFFDFSIQDSGSGINQEDIDRLSGSFSSSTRNIL
                     TRKTEGLGIGLSIVKDYTEILGGAVSFQKKKNGTLVSLKIPLALTMEKIWYYDLKKDF
                     TKFSMQGFDTIGELNSYNTDLKGVMSYIKKQFEEYQSIDISSKKTFVTKALTDLLRLS
                     KEKNLKTLEKITLQILSILKSDKDAYAYDLLDTLYKRINFSFSE"
     gene            3345..4085
                     /locus_tag="PLU28_07485"
     CDS             3345..4085
                     /locus_tag="PLU28_07485"
                     /inference="COORDINATES: protein motif:HMM:NF024230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HOG34941.1"
                     /translation="MFLERISDVLIYIAKNLSFYGASATEIEYYISKISKAYDIDVNI
                     AAIGTVITLTVVDDHGKSITRIERVSYSEINFEKLSLWENMIKRVTEEKPTIFTLKQW
                     IKDIEKKENLLGISFWKNIIAVFIASFGFCFVFRGNYTDAVASGISALLVYMATYKIT
                     SKIFRDFLAGLLVYSVIKLISKIVSISIFPSLAGGIMVFVPGLMLTNAIMEIGDRNLV
                     SGSSKLMEAIFMLGSLVLGAAMASALWG"
     gene            4095..4547
                     /locus_tag="PLU28_07490"
     CDS             4095..4547
                     /locus_tag="PLU28_07490"
                     /inference="COORDINATES: protein motif:HMM:NF018448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HOG34942.1"
                     /translation="MDTEIITRILMAFLATGGFSFLFKTPKRLLPIASLTGGLGWAAY
                     EVIKLFYGFESAIILSSAVVGFLSHIFAKVFKTNVHPFITAGIVPLVPGASAFFAVKS
                     FIDKNQSQASNFAYETFVSAFGIVIGIAISSYVSKILNKKDIDTEKTI"
     gene            4578..4652
                     /locus_tag="PLU28_07495"
     tRNA            4578..4652
                     /locus_tag="PLU28_07495"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4611..4613,aa:Arg,seq:cct)
     gene            complement(4725..>5955)
                     /locus_tag="PLU28_07500"
     CDS             complement(4725..>5955)
                     /locus_tag="PLU28_07500"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOG34943.1"
                     /translation="VIGMWFAGVPTKELDDSLRKGILSIILYISIISFALILVFVFFI
                     SRILKRPLNKLTASISALAGYDLSEKSVQEIKQYTKRKDEIGKISSCLSDLHENLLSI
                     LNNIKSSSEKINSSSSDLSKLAMNSGKASEELQEGAENILLNVKSTSESIEDANNGVS
                     EVASSAQNVSHLAQNLSENSVMVFEASKKGLGSIKTIVNRISQARDQAKDTIDAVKDV
                     EAKSQNIGDVVGKISSIAEQTNLLALNAAIEAARAGESGKGFAVVAEEIRKLAEESKR
                     TTQEIESILGQIRTGIKGVDAVTQSTVKTIYEISESSVDIEKEFGQIIENIKHISTSV
                     ENLSANSQEQSASSQEIAGSIDASVKASKEITEQVNEMTSSIRLQSESAQEVSVNSDN
                     LNSLASELAGIISKFKF"
BASE COUNT         1722 a         1109 c         1102 g         2022 t
ORIGIN      
        1 atgccttttc cctgtcctgt ctgaacttgc tccagaactg aaaagcacat acctgagcga
       61 gagtatagtc tatataatag aaaggatctt tatatatgtg agcctgtctc tgccagaatc
      121 ctccgttgac aagataaccg atagcgtcat aatctctgtg tggcagatat tttttttcaa
      181 tttcggacca cttttcacgc ctttgcattg gggttgcctg aggattttca tatacaaaat
      241 gctggaactc gtcgacagat acgccgtaag gaataaaagt caatgcactt gccagatgca
      301 tatatcttgc tttttcagcg ttttcagtat agaatagttc ccaccacggc caagttataa
      361 actccatgct cattgaatgt atttcgcatg cctcaagtgt aggccagcca tactccggaa
      421 tcttgaagtc ctttgaggaa tatacctgaa aagcatgtcc catctcgtgt gtgagaacat
      481 ccacatcacc ggaagttccg ttgaaatttg aaaatataaa cggtgactga taatcaggaa
      541 taaatgtgca gtatcctccg ggagcttttc cttcctttgc aaccagatca aaaagctcgt
      601 tttcaatcat gaaatcaaaa aaatctcctg tttctctgga aagttctcta tacatcttct
      661 gggctttttt cacgagatcg tcatatgtac ctataggctt tggattgccg ccttcaaaag
      721 taaaagattc atcataatag aacagatgat ctattcctat tctgtcttgc tgcttggaaa
      781 agattttaga ggccatagga actatatatc tgcggacctg ctctctgtag tttgctacca
      841 taacggggtt atagtcagtt ctccccagtc tgtcgtagcc taactgtaca aagtttttat
      901 agcctaactt tctggctatg gtgtctctca ctttcacaag ttcgtcgtat ataccgtcaa
      961 actcttcaag atgttcctca aaccatttaa atttggcctg tacagctttt tttctggtct
     1021 gcctgtcggg tgactgctca aaaggcgtca tctgtgaaag gtttctttcc tttccttcaa
     1081 acataatttt agctgatcca agaagcttgg tatactttga agtaagcttg ttttcactct
     1141 gcaggtcatt tattatttcc ggcgaaaagg ttttcaggga aaggtctgca agcgcaaaaa
     1201 gctgttttcc ccatttttct tccagttctt tgcgaaaatt tgatttaacg agctctttat
     1261 aaaaactatt gttaagttct tcaaaaagcg gcccgttttc atcaaagaag ttcatttctt
     1321 cttcataaaa ggagtctttg gtgtttattg tagcgcgtac attcactaaa acctgcatgg
     1381 tcatgaagct tcttctcaga tcatttattt tttccataaa accgctttgt tcctggaagg
     1441 aactggcctg tgaaaaggac tttataagct gttcaaactt tgctttggtg gagttgaagt
     1501 ccggacggac atacttcatt tcactgaatt tcatatttat cccccttttt tttgaatata
     1561 atgatattct atcataaaaa ttttaataat aaaaaaaaat ttattggata ataaaaaaac
     1621 tatcagtttt cactgatagt ttatggctcc ggatgagggg ctcgaacccc cgacctaatg
     1681 gttaacagcc atccgctcta ccgctgagct aatccggaat atataagtaa gacataaatt
     1741 attatagcac cggacacaac gatttgcaag aaaattttat gttaagtaat ctttaaatat
     1801 agtagtacca cattattctg aaaaagagaa gttaatccgt ttataaagag tatccagaag
     1861 gtcataagca taagcatctt tatctgattt taggattgaa agaatctgaa gagttatttt
     1921 ttcaagggtt ttaagatttt tttccttgct caggcgtaaa aggtctgtca aagcttttgt
     1981 tacaaaagtt ttttttgatg agatatctat actctgatac tcttcaaact gtttttttat
     2041 atatgacata actcctttta aatctgtatt gtaagagttg agttctccaa tcgtatcaaa
     2101 tccctgcatg gaaaatttgg taaagtcttt tttaaggtcg tagtaccata tcttttccat
     2161 agtaagtgcc aagggtattt ttaaacttac cagagtaccg ttttttttct tttggaagga
     2221 aactgctcct ccgagaatct ctgtataatc cttgacaatg ctcagaccaa tgcccaatcc
     2281 ttcggttttt cttgtaagta tgtttctggt acttgaagag aaggatccgc taagcctatc
     2341 tatatcctcc tgatttattc cgcttccgct gtcttgtatc gaaaaatcaa agaaagattc
     2401 ctgagcgttg aactcaaacc atatatcgcc ggtgtctgta aatttcaccg cattgtctaa
     2461 aagctcttcc agaatccttc ttagtttttt gctgtcgaca aagatggttt tttcatcgta
     2521 agtaaaagga gcatgaatga agtgaaacga aagtcctttt ttgaagcata ggttttcgta
     2581 tttggatgag atttccagta ccagattacc tggattgaaa tcttcaagaa gcataaactt
     2641 gtttatggaa ttttcttcaa gataggatct ttctatcagc ctgtcaaatt ttgccaaaag
     2701 ttcttttgaa ctgtctatca taacccccag catctgcagc tgcattttgt ctgagagctt
     2761 gctttttaaa tgctctgaaa aaccgattat agattgcaga ggggttttta tttcatgtga
     2821 aagtatcttt aaaatattgg ttttaaagcc tgcggaattt ttaattcttt cctgtgagtc
     2881 ctgaagtgct tgtatggagc ggttgaagtt ttcatagagc tgttcaaact cttcaagtat
     2941 agttttgaac tcatcattgt tctgatactc aacagaaggc tttttaccgg ttttaatttt
     3001 ttgaaggtaa gaaagaatgt agtctatcgg ctggctgatg tcctttttta tctgatgtct
     3061 tagaaaaaat agaaaaccgg agaataccaa actgaaaaaa acaatccccg ctgtcagatg
     3121 aagtatgaaa aagttcatac tctcaggtgg caatgtattt tcatgaagat aacttttgat
     3181 gaccgcagca gcaacaatat gaactataaa aagtaatata agagtatata taataaaggt
     3241 agaggtaatt tttttattga tttttgaaga aaatgagctt ttattgtctt taatcatata
     3301 taataacccc caaataataa tggatacttt cctcaggagg aaaaatgttt cttgaacgga
     3361 tatctgatgt acttatatat atagcgaaaa atctttcttt ttacggagct agtgcaacag
     3421 aaatagagta ctacatatca aaaatttcta aggcatacga tatagatgtg aatattgcag
     3481 ccataggtac tgtcataacc cttacagtgg ttgatgatca tggaaaatca ataacaagga
     3541 ttgaaagagt aagctattcc gaaatcaact ttgagaagct ctcactatgg gaaaacatga
     3601 taaaacgtgt aacagaagaa aaaccaacca tattcacact gaaacagtgg ataaaggata
     3661 ttgaaaaaaa agaaaattta ctgggaataa gtttctggaa aaatattata gccgttttca
     3721 ttgcatcttt tggtttttgt tttgtattcc gtggaaatta tacagatgca gtagcctcag
     3781 gtatttctgc tcttttagtt tatatggcta catataagat aacaagcaag atattcagag
     3841 actttcttgc cggattgctt gtatactctg tgataaaatt aatatcaaag attgtgtcaa
     3901 taagcatatt tcctagcctt gcaggaggta ttatggtctt tgtgccgggg cttatgctta
     3961 caaacgctat tatggaaata ggagacagaa atctggtttc cgggagctcc aaacttatgg
     4021 aggccatatt tatgcttgga tctttggttc ttggagccgc tatggcttct gcactatggg
     4081 gataaggagg agatatggat acggaaataa taaccagaat tcttatggct tttttggcga
     4141 ccggaggatt ttctttcttg tttaaaactc ctaaaagact tttgcctatt gcttctttga
     4201 caggcggact aggatgggca gcctatgagg tgataaaact tttctatgga tttgaaagcg
     4261 ctataatcct ttcttcagct gttgtaggat ttctttctca catatttgcc aaagtattta
     4321 aaaccaatgt acaccccttt ataacggcgg gtatagtacc gcttgttccg ggagcctccg
     4381 ctttttttgc tgtaaaaagt tttattgaca agaatcagtc acaggcctcg aacttcgcat
     4441 atgaaacttt tgtgtctgct tttggtatag taatagggat agcaatttca tcgtatgtat
     4501 ctaaaatact gaataaaaaa gatattgaca cagaaaaaac aatctgatat aatttaacgt
     4561 ataaaaaata ataccgtgcc ttcatagttc aacggataga acggtggatt cctaatccgc
     4621 agataggtgt tcgattcacc ttgggggcac catatcctta agtgcaaaag tgccggaata
     4681 ttccgacact tttgcactta taattatatt ttagcataat ttgttcagaa cttaaactta
     4741 cttatgatac ctgccagttc agatgcgaga ctgttgaggt tatcactgtt tacactgacc
     4801 tcttgtgcgc tttctgactg taatcttata gaggaagtca tttcattaac ctgttcagtt
     4861 atttcttttg aagctttaac ggaagcatct attgagccag ctatttcctg tgatgaggcg
     4921 ctctgttcct ggctgtttgc agagagattt tctaccgagg tacttatatg ttttatattt
     4981 tctattatct gtccgaactc tttttctata tcaacagagc tttcagatat ttcatatatg
     5041 gtttttactg tgctttgggt tactgcatca acacctttta ttccggttcg tatctgtcca
     5101 agaatgcttt ctatttcttg ggtagtccgt ttgctttctt cagccagctt tcttatttcc
     5161 tcagcaacca cggcaaagcc tttgcctgat tcgcctgctc ttgctgcttc tatcgctgca
     5221 ttaagtgcaa ggaggttagt ctgctcggca atagaactta tctttcctac tacatctcct
     5281 atattctgtg actttgcttc aacgtccttt actgcatcaa tggtgtcctt agcctgatct
     5341 cttgcctggc ttatcctgtt cacaatggtt ttaatgctcc ccaacccttt ttttgaagcc
     5401 tcgaatacca ttaccgaatt ttcagataga ttctgtgcca aatgcgaaac attctgagcc
     5461 gaagaggcta cttcagaaac tccgttattt gcatcttcaa tagattcaga ggtggattta
     5521 acgttcagaa gaatattttc ggcaccctct tgaagttctt cgctcgcctt acctgagttc
     5581 atagcaagtt ttgaaagatc cgaggaggag gagttaatct tttcagatga tgattttata
     5641 ttgttgagga ttgaaagaag attttcgtga agatctgaaa gacatgatga gattttaccg
     5701 atttcgtcct ttcttttagt atactgtttt atttcctgta cactcttttc agaaagatcg
     5761 tatcccgcaa gagccgatat ggaagcagtg agcttgttca acggtcgttt caatattcta
     5821 ctaatgaaaa atacaaacac aaggataaga gcaaaagaga ttattgaaat atataaaata
     5881 atggataaga taccttttct gagagaatca tcaagctctt ttgtaggtac tccggcaaac
     5941 cacattccta ttacc
//
LOCUS       DAOSHR010000125         5953 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_11757_length_5953_cov_5.489658, whole genome shotgun sequence.
ACCESSION   DAOSHR010000125 DAOSHR010000000
VERSION     DAOSHR010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 5953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5953
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11757_length_5953_cov_5.489658"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /gene="rplD"
                     /locus_tag="PLU28_07505"
     CDS             complement(<1..188)
                     /gene="rplD"
                     /locus_tag="PLU28_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOG34944.1"
                     /translation="MAKVDVINSNGQVVGAIELKDTVFNIEPNMDVMYRYVDMQLSNA
                     RSGTASTLTRAEVAGGGR"
     gene            complement(225..848)
                     /gene="rplC"
                     /locus_tag="PLU28_07510"
     CDS             complement(225..848)
                     /gene="rplC"
                     /locus_tag="PLU28_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOG34945.1"
                     /translation="MKGIIGKKVAMTRIYKDGKAIPVTVIKAGPCVVSQKKTVETDGY
                     NSIQLAFEEVAERKLTKPQLGHFKKANIKPMRKLREFRTDDVDSYQVGQIVDVSVFQE
                     GDIIDVTGWSKGRGYSGGMKMWNFSGGEASHGSKFHREGGSTGQHTYPAEVFKGKKMF
                     GQYGNERVTIQNSQVVKIDAENNLIAIKGGVPGARGGLVIIKDAVKK"
     gene            complement(877..1185)
                     /gene="rpsJ"
                     /locus_tag="PLU28_07515"
     CDS             complement(877..1185)
                     /gene="rpsJ"
                     /locus_tag="PLU28_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOG34946.1"
                     /translation="MGKKFIKIKLKAYDSRLLDESAKKIIEAVKEANAKVSGPIPLPV
                     ERSVYSVVRSPHKYSYSMEQFEKRVHKRVIYIYDASSETVTNLLKVNIPSGVAVDIKA
                     "
     gene            complement(1290..2489)
                     /gene="tuf"
                     /locus_tag="PLU28_07520"
     CDS             complement(1290..2489)
                     /gene="tuf"
                     /locus_tag="PLU28_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HOG34947.1"
                     /translation="MAKEKYLRNKPHMNIGTIGHIDHGKTTLTAAITKSLSLKGFADF
                     TPFDQIDKAPEEKARGITISVSHVEYQTDNRHYAHIDCPGHADYIKNMITGAAQMDGA
                     ILVVAATDGVMPQTREHVLLARQVNVPAIVVFVNKVDAVDDPELVDLVEMEVRDLLSS
                     QQFPGDTLPVIRGSALNALNANSQDDPWVKKIYELMDAVDNYFPEPKRLADQAFLMAI
                     EDVFTITGRGTVVTGRIERGTIKVGEEVEIIGIKEENKKSVCTGVEMFRKELDSGMAG
                     DNVGCLLRGVDKDDVERGQVLAKPGSIHPHKKFRAQVYVLKKEEGGRHTAFTVGYRPQ
                     FYVRTADITGSLVAFDGGAEMVMPGDNIIMTIELIHPIAIENNMRFAIREGGRTIGSG
                     VVTEIIE"
     gene            complement(2542..4626)
                     /gene="fusA"
                     /locus_tag="PLU28_07525"
     CDS             complement(2542..4626)
                     /gene="fusA"
                     /locus_tag="PLU28_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOG34948.1"
                     /translation="MKERKFALQNLRNIGIIAHIDAGKTTTSERVIFYTGRKHKIGNV
                     DEGNTTLDWMEQEKERGITVTSAATTCFWKGFRINLIDTPGHVDFTIEVERSLRVLDG
                     AVAVFDAQVGVEPQSETVWRQADKYQVPRLAYMNKMDKIGADFFNAINTMKVKLGANP
                     VAVQVPIGAEASFRGVVDLLKMKAIYWDNEDSTQMHEEEIPQDLVKICEEKREDLISA
                     VSEFDEEIMDLYISGEEIPTDKIKQAIRKGTIANSLVPVYCGSSFRNKGIQPILDGVI
                     DYLPSPLDLPPVKAFDARTKEFVQDIHPDENGPFIAMAFKIMVDPYVGKLTFARVYSG
                     KLEKGNYVLNTTKGVKERISRLMFLHADQREEVDYITTGDIVGIIGLKDTTTGETIAQ
                     EGSDLILEKLEFPEPVISVSIEPETKDDEQKLTKALQALLDEDPSLRVNVDHDTGETI
                     LHGMGELHLDIIVDRIKREHKVTVRVGKPQVAYRETIKVGADAEGKYIRQSGGKGQYG
                     HCKIKIEPLPANSEKTYEFVDKVVGGTIPKEYIPAIGEGIREAMQGGIVAGYPMVGVR
                     ATVYDGSYHDVDSSEMAFKIAGSMAFKDAAKRAKPVLLEPIMKVDVTTPEEYMGDIIA
                     DLNSRRGRIEGFDNVGNTTTRIIQAIVPFSELFGYATAMRSLSQGRATNTIQFSHYDE
                     VPASVAEKLITK"
     gene            complement(4642..5109)
                     /gene="rpsG"
                     /locus_tag="PLU28_07530"
     CDS             complement(4642..5109)
                     /gene="rpsG"
                     /locus_tag="PLU28_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOG34949.1"
                     /translation="MRRRRAEKRVVAPDPIFGDVLVTKLVNKIMQSGRKAKAESIVYG
                     AIDVLKQKTGEEAIVAFRKAIENVKPLIEVRPRRIGGATYQVPFEVPEEKSVALALRW
                     VVNSARSRHGKPMIEKLSQELLDAYNNTGSAVKKRDDVHKMAEANKAFAHYKW"
     gene            complement(5125..5526)
                     /gene="rpsL"
                     /locus_tag="PLU28_07535"
     CDS             complement(5125..5526)
                     /gene="rpsL"
                     /locus_tag="PLU28_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOG34950.1"
                     /translation="MPTINQLIRFGREKVVYKSKAPALQHNPQKRGVCVRVSTMTPKK
                     PNSALRKIARVRLSNGIEVTSYIPGEGHNLQEHSNVLIRGGRVKDLPGVRYKIIRGTL
                     DAAGVENRKQSRSRYGAKKPKENKAKAAPKK"
     gene            complement(5666..>5953)
                     /locus_tag="PLU28_07540"
     CDS             complement(5666..>5953)
                     /locus_tag="PLU28_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOG34951.1"
                     /translation="HGAKGLEWKVVILISVNPGDLPNAMAIKEMKLDEEERLFYVAVT
                     RAKDELYLLNQSTGVTNPYLKNSFVFVKSENDFMKKIPKEKVTKLKTGYDF"
BASE COUNT         1470 a         1417 c         1022 g         2044 t
ORIGIN      
        1 tttcgtccgc ctccggctac ttctgctcga gtcaatgttg aagctgtacc tgaccttgca
       61 ttcgaaagct gcatatcaac gtatctgtac ataacgtcca tattcggctc gatattgaat
      121 acagtgtctt tcaactcaat ggctccaaca acttgcccat tactgttgat tacatcaact
      181 tttgccattt tgttctcctc ctatacacgt tttcgggcgt ataattattt cttcactgca
      241 tccttaatta ttactaaacc gcccctcgct cctggaactc caccttttat tgcaataagg
      301 ttgttttctg cgtctatttt tacgacttga gagttttgta ttgtgactct ttcatttcca
      361 tactgcccaa acatcttctt gcctttgaaa acctcagcag gataagtatg ctgaccggtt
      421 gaaccgcctt ccctatggaa tttggaaccg tgagaagctt ctcctccgct gaaattccac
      481 attttcattc ctccggaata gccgcggcct tttgaccagc cggttacatc aattatatcg
      541 ccttcctgga atacggaaac atcaacaatc tgaccgacct gatagctgtc tacatcatct
      601 gttctgaact ctcttaactt tctcatcggt ttgatattag cctttttaaa atgaccaagc
      661 tgaggcttgg taagtttcct ttccgcaacc tcttcaaaag ctaactgtat tgaattgtaa
      721 ccgtcagttt ctacggtttt tttctgtgaa accacacagg gtccagcttt tataactgtg
      781 accgggatag cttttccatc tttgtaaatt cttgtcatag ctaccttttt acctattata
      841 cctttcatgt tttacacctc cagagcccga tctgttttat gcttttatat ctactgctac
      901 tcctgacgga atatttactt ttaataagtt ggttactgtt tcacttgagg catcataaat
      961 gtatataact cttttatgaa ctctcttttc aaactgctcc attgaatagg aatacttatg
     1021 gggtgatctc acaacagagt ataccgatct ttcaacggga agcggtatcg gacctgaaac
     1081 tttcgcattg gcctctttta ctgcttctat tatctttttg gctgattcgt ccaacaaacg
     1141 ggagtcatat gctttaagct ttatttttat aaactttttg cccatttctg ctttttccct
     1201 ccttaaaaat ccgaacaata gggggagcat aaccgctccc cggctgtcct cgaattattt
     1261 taactttcaa ctgttgcagt tacgttgtat tactctataa tttctgttac aactcctgaa
     1321 ccaattgttc ttccgccttc ccttatagca aatctcatat tattttcaat tgctatagga
     1381 tggatgagtt ctattgtcat tattatgtta tctccgggca taaccatttc tgctccgccg
     1441 tcaaaagcaa ccaacgaacc ggttatatcg gctgttctca cgtagaactg aggtctgtat
     1501 cctacggtaa atgcggtgtg ccttccgcct tcttctttct taagaacgta aacctgagcc
     1561 ctaaactttt tgtgcggatg aattgaaccc ggctttgcaa gaacctgtcc tctttctaca
     1621 tcatccttgt ctacacctct gagaagacat cctacgttgt ctcctgccat acctgaatca
     1681 agttcttttc tgaacatttc aacaccggta catactgact ttttattttc ttcttttata
     1741 cctattattt cgacttcttc tccaacctta atggttcctc tttcaattct tccggtaaca
     1801 actgttcctc ttccggttat ggtgaataca tcttcgattg ccataaggaa tgcctggtct
     1861 gcaagtctct taggctctgg gaagtagtta tcaaccgcat ccataagctc ataaatcttc
     1921 tttacccacg gatcatcctg cgagttggca ttaagagcat taagggcaga tcctcttata
     1981 actggaagtg tatcgcctgg gaactgctgg gaagaaagaa ggtctctgac ttccatttct
     2041 acgagatcaa cgagttctgg atcatcaaca gcgtcaactt tatttacaaa aacaactatt
     2101 gcaggaacgt taacctgtct tgccaaaaga acgtgttctc ttgtctgggg cataacaccg
     2161 tcggtagcgg caacaacgag aattgcaccg tccatctggg ctgctccggt aatcatgttc
     2221 tttatgtagt cagcgtgacc tggacagtca atatgtgcat agtgtctatt gtctgtctgg
     2281 tattcaacgt gggaaacact tattgtaata cctcttgctt tttcctcagg agccttatcg
     2341 atctgatcga atggggtgaa atctgcaaat cctttcaaag aaagagattt ggtaatagcg
     2401 gctgtcaaag ttgtctttcc gtggtcaatg tgtcctatgg ttcctatgtt catatggggt
     2461 ttatttctga gatacttttc ctttgccatt aggattccct ccttgttttt aaagaaatta
     2521 tatttataat agataaacca tttatttggt tataagcttt tctgccactg aagcaggtac
     2581 ttcatcgtaa tgcgagaact gtatcgtgtt tgtggctctt ccctgagaca atgatctcat
     2641 agctgttgca tatccgaaaa gttctgagaa aggtacaata gcttgaatta tcctagtagt
     2701 tgtattaccc acattatcaa aaccttctat tcttcctctt ctggaattga gatcggcaat
     2761 tatgtcaccc atgtactctt caggagtggt aacgtctact ttcattatcg gttcaagcag
     2821 aacaggcttc gccctttttg cagcatcttt gaatgccatg gatcctgcaa ttttaaaagc
     2881 catttctgaa gagtcgacat catgataaga accgtcataa acagttgctc ttacccctac
     2941 cataggatag cctgccacta taccgccctg cattgcttca cgtattcctt ctccgatagc
     3001 cggtatatac tctttgggaa tagtaccgcc aacaacttta tccacaaact cgtatgtctt
     3061 ttcactgttt gccggaaggg gttcaatctt tatcttacaa tgaccgtact gtccctttcc
     3121 tcctgactgc cttatatact ttccttctgc atctgcgcca acctttatgg tttctctgta
     3181 ggcaacctga ggctttccga ctcttacagt tactttatgt tctcttttta ttctgtcaac
     3241 aattatatca agatgtagtt cgcccatacc atgcagtata gtttcaccgg tatcgtgatc
     3301 tacatttacc ctgagactcg gatcttcgtc aagaagagcc tgaagtgctt tggtaagttt
     3361 ctgctcgtca tctttggttt caggttcgat agaaacggat ataacaggtt ccggaaattc
     3421 aagcttttca agaataagat cagaaccttc ctgagctatg gtttctccgg tggttgtatc
     3481 tttaagacct attataccga ctatatcacc ggtagtaata tagtcaacct cttccctctg
     3541 gtctgcgtgc aggaacataa gcctggatat tctttccttt acgcctttgg ttgtattgag
     3601 aacatagttt cctttttcaa gcttgcctga gtaaactctc gcaaaagtaa gctttcctac
     3661 atacggatca accattatct tgaatgccat agcaataaaa ggaccatttt catcaggatg
     3721 tatgtcctgt acaaactctt ttgtcctggc atcaaaggct tttacgggag gaagatccaa
     3781 cggagacggc aaatagtcga ttactccgtc aagaataggc tgaattcctt tgtttctgaa
     3841 tgacgaacca cagtatacag gaactaggct gttagctatg gttccttttc ttatggcttg
     3901 ttttatctta tctgtaggaa tttcttcgcc ggaaatataa agatccatta tttcctcatc
     3961 aaactcagaa acagcagaaa taagatcttc acgtttttcc tcgcaaatct ttaccagatc
     4021 ctgaggaatt tcttcctcat gcatctgtgt gctgtcctcg ttatcccagt agattgcctt
     4081 catcttaagg agatcaacta cacctctgaa agatgcttcg gctcctatcg gaacttgaac
     4141 agcaacagga ttagctccca gtttaacctt catagtattg atggcgttga agaaatctgc
     4201 tcctattttg tccattttgt tcatgtatgc gagacgggga acctggtact tgtcagcctg
     4261 tctccagact gtctcggact gcggttcaac ccctacctga gcatcaaaaa ctgcgaccgc
     4321 tccgtcaagt actctaagcg acctttcaac ttctatagta aaatccacgt gacctggtgt
     4381 atcaattaaa ttaatcctaa aacctttcca aaaacatgtg gtagccgcag aggtaacggt
     4441 aattcctctt tccttctctt gttccatcca gtcaagagtt gtattacctt cgtcaacatt
     4501 accaatctta tgtttacggc cggtataaaa tattactctt tcggacgtag tcgtcttacc
     4561 ggcatcaatg tgggctatta tcccaatgtt tctaaggttt tgcagggcga acttcctctc
     4621 tttcaaaaca accgcctcct attaccactt atagtgagcg aaggccttat tggcttcagc
     4681 catcttatgc acatcgtctc ttttcttaac tgctgagcca gtattattat aagcatccaa
     4741 taattcctga gacaacttct caatcatggg ttttccgtgt cttgatctgg ctgaattaac
     4801 aacccatctc aaagccaaag ctactgattt ttcttcagga acttcaaacg gtacctgata
     4861 cgttgctcct ccgattcttc ttggtcttac ttctattaat ggcttaacgt tttctatagc
     4921 ttttctgaat gcaactattg cctcttcgcc tgttttctgc ttcaacacat ctattgcacc
     4981 atacacaatg gactcggcct tggcctttct tccactctgc attattttat tgacaagttt
     5041 tgtaacaagg acatctccaa atataggatc gggagctaca actctttttt ctgctcttct
     5101 ccttctcatt tatccaatac ctccttattt cttaggtgct gctttagctt tgttttcttt
     5161 tggcttcttt gctccatatc tgcttctgct ctgttttctg ttttctactc cggcagcgtc
     5221 gagagttcct cttataattt tatatctgac accgggcaaa tccttaaccc ttccgcctct
     5281 tataagaacg ttggagtgtt cctggaggtt atgcccttca cccggaatat atgaagtaac
     5341 ttctattcca tttgaaagtc ttacccttgc aatttttctt aacgctgagt tcggtttctt
     5401 aggggtcatg gtcgaaaccc ttacacaaac tcctcgcttc tgcggattgt gctgaagcgc
     5461 aggcgctttt gatttgtaga ctaccttctc tctaccaaat cgaatcaatt gattaatcgt
     5521 cggcatctgt atttatgccc tcctttctat aatttttcgt aaaaatacca tgttctattt
     5581 tacctcatac ttaattaatt atatgttaaa atgtcataac aagttttgca gatacaataa
     5641 tgatgatcgc acaccagcaa gttcatcaaa aatcatagcc tgttttaagt tttgtaactt
     5701 tttctttcgg tatttttttc ataaaatcat tttcgctttt cacaaaaaca aaactattct
     5761 tcaaataagg atttgtaact cctgtactct gatttaagag gtacagttca tctttcgccc
     5821 tggtaacggc aacataaaaa agcctttcct cttcgtcaag cttcatttcc ttaatggcca
     5881 tagcattagg aagatctccg ggattgacag atataagtat gacaaccttc cattccagac
     5941 ccttggctcc atg
//
LOCUS       DAOSHR010000126         5943 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_11789_length_5943_cov_4.888757, whole genome shotgun sequence.
ACCESSION   DAOSHR010000126 DAOSHR010000000
VERSION     DAOSHR010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 5943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5943
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11789_length_5943_cov_4.888757"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="PLU28_07545"
     CDS             complement(<1..466)
                     /locus_tag="PLU28_07545"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF21 domain-containing protein"
                     /protein_id="HOG34952.1"
                     /translation="MDPSGSSSPYLVWQIISIFFLLLLSAFFSSAETTLSTIGKLKIK
                     EMMEKREDRFAGIEDYFAKQKQYLTTILIMNNIVNIVASSLTTITLVSIFPGDNGRVV
                     AISSGLMTLLILTFGEITPKVYARENAEKLFKFSYHIINILNIILTPVVWVFI"
     gene            complement(490..1389)
                     /locus_tag="PLU28_07550"
     CDS             complement(490..1389)
                     /locus_tag="PLU28_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HOG34953.1"
                     /translation="MGLKLRRPVDVRRTAADYIIITAGTFFTALGIVVFLVPFNIIAG
                     GVSGLSMVTNYLFNWWIGTQMLIYNMFLFILAFWLLGVGFGVKSIYSAVTLSVFIDLL
                     QQGFKLDKLMLGITSSHSVDMTLLSAVYGAVITGFGVGIVIWRGATTGGTDILAMIMN
                     KYLSISVGTSLIIVDSVITALSLIINPLIPMYGLITIFISSKVIDAVVDGFESTRTVL
                     IISDYYDKIKERIYTDLDRGVTYLKGTGTYTNKDKNIVMVTVPRTEIGMLKKIIKEVD
                     PVSFTTVLSTNETIGYGFKKITK"
     gene            complement(1431..2567)
                     /gene="dnaJ"
                     /locus_tag="PLU28_07555"
     CDS             complement(1431..2567)
                     /gene="dnaJ"
                     /locus_tag="PLU28_07555"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOG34954.1"
                     /translation="MPQKKDYYGTLGVSRDASLDDIKKAYRLLVKEWHPDRHFEDKKV
                     AEEKFKEIQEAYEVLSDPEKKKLYDRFGFVPENGGMPNSDGYSGSGGFEDVFKDIFGE
                     GRAGAGPFSDFFDMFFNESSSKSQRRTSQPPQKGEDIRVNVTMDMREVLEDTQKTIEY
                     NKYEACPACHGNGSEHGTSFRKCPRCNGEGTIHEEQRTFFGVFVKTYPCPTCNGTAKI
                     VEKKCSVCSGAGKTFKNEKFVITIPSGVEDGFILRVTGKGNSGKNGGPNGDLLVGIRV
                     NPDKRFIRKGTDLETQVDIDYLQALLGDRIKIPTLEGDVYEKISEGTSPNTVVRLKGL
                     GIPDFRTKKRGDIYVRINVKIDKPSIREKKLLKELAQMRGISAE"
     gene            complement(2567..3196)
                     /gene="grpE"
                     /locus_tag="PLU28_07560"
     CDS             complement(2567..3196)
                     /gene="grpE"
                     /locus_tag="PLU28_07560"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOG34955.1"
                     /translation="MSQDIKKEQEQVINEQSSVGETAEKKECQNNTPEYAELEKSKER
                     ISELEQEVMKFRNAAIQLKMDFESFKDIVDREKKNIEIKTKEDTLKKFIPVFEKFSIS
                     MKYSDGSDFAKAMEMIFRDFINAFEKCGLTFIHPEKSDQFDPFEHEVIDKCETSDVSE
                     YHVFETKTYGYKLNGTVLRAAQVVVAVKPKEKKEEVKTECTEKSEEGEK"
     gene            complement(3219..4238)
                     /locus_tag="PLU28_07565"
     CDS             complement(3219..4238)
                     /locus_tag="PLU28_07565"
                     /inference="COORDINATES: protein motif:HMM:NF013768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34956.1"
                     /translation="MGPDLTERQKDVLMAIVTLYIKNQKPVSSDDVLSNTRMNTSSAT
                     VRNDMQKLQHLKYIFQQHTSGGRIPTDRAVKLYFDVVKELYSNSSSVLDIPKEYKFYD
                     LNLMFENVSKMISNTLESLVIFEYPNPRYLYITRAVVTPVTESHCVITLLTSLGLTIS
                     RTMEIYDLPHPKELESLLNKGLTGKSLSEVFSAVKLQSVQAQDERVINMFKLVEGLVN
                     EFSRIKYTTNGFDILISKNRPPIESIEMLACIVGNDKIKEELFRNLTANRDINLCLGN
                     ELVTGETYRPLKNFAFFYTSYCNGVNPVGRIFVITNKFSDYEKNYKVINEYTSRFSEI
                     ISKNL"
     gene            4440..5942
                     /locus_tag="PLU28_07570"
     CDS             4440..5942
                     /locus_tag="PLU28_07570"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOG34957.1"
                     /translation="MITLNQVIECLKGQTVKKRVPEQLERELYGLTSNSKETRKGDIF
                     VAVKGPKVDGHDYIDIAVKHEAGLVILQNKSKIPPELQFDYILVKDTRKALADIAYLM
                     ADVDPKDFRFFTVTGTNGKSTCVCLLHHLMRESHRDSVLMSTVEIKVNDELIDEPYNT
                     TPGVLEIADVLKKAKENKIDFINLEISSHGIAQKRIENLKFDVISFTNITRDHLDYHK
                     DFEEYEATKLSLVDYLKPNGIASVNIDEIDVNKIRLPSERIITYGQSEKADYRIKDIN
                     CNIYQMNFKIQTPQGNDISVYTSIIGEFNAYNITNTVIAAKYFGLTDEEIKHGIITFK
                     GVPGRFQLVPTSKSLGFSVVVDFAHTPDALEKVLKTARMVSKGRVIIVFGAGGNADIG
                     KRLMMGEVTSRYADIVVITNDDPKDEEPEKIIEDIKQGLDVRKNFIVIPDRKTAIDAA
                     INFANRDDIVVIAGRGHEKFQLFSNGKKIKFNDYEVAGEIIENLKRSMKK"
BASE COUNT         1811 a         1242 c         1058 g         1832 t
ORIGIN      
        1 ttataaatac ccaaaccaca ggggtaagga tgatattaag aatatttatt atgtggtatg
       61 aaaacttaaa aagcttttct gcgttttctc tcgcatatac tttcggggtt atctcaccaa
      121 aagtaagtat cagcaaagtc atcagtcctg acgatatagc cacgactctt ccgttgtctc
      181 ccggaaaaat gcttacaagc gttatggtgg taagagacga agcaacaatg ttcactatat
      241 tgttcattat aagaatggtg gtaagatact gtttttgttt tgcaaagtaa tcctctattc
      301 cggcaaacct atcctccctt ttctccatca tctcttttat cttcagtttg cctatggtgc
      361 taagagttgt ttctgcagat gagaaaaaag ccgacaaaag caaaaggaaa aaaatggata
      421 taatctgcca tactaagtat gggctgctac tgcccgaagg atccatataa cacctcacct
      481 aatataaagt tatttggtta tttttttaaa gccgtatcct atggtttcat ttgtagaaag
      541 tactgttgta aacgaaacag gatcgacctc ttttattatt ttttttaaca tacctatctc
      601 tgtacgagga acagtcacca ttactatgtt cttgtccttg ttagtatatg ttcctgtgcc
      661 ttttagatag gtgacacccc tgtcaagatc tgtatatatt ctttctttaa ttttatcata
      721 ataatcacta ataatcaaaa cagttcgtgt ggactcaaat ccgtccacaa ctgcatcaat
      781 taccttactt gagataaaga tagttattaa cccatacatt ggtatcagag gatttatgat
      841 aagcgaaagt gccgttatca ccgaatctac aattattagg ctggttccta cggatatgct
      901 gagatactta ttcattatca tagccaagat atcagtacct ccggtggtcg ctcccctcca
      961 tatcactatt cctacgccaa agcccgttat tacagctccg tagaccgcac ttaacagggt
     1021 catatcaaca gagtgcgaag aggttatccc aagcatgagt ttatcaagct taaaaccctg
     1081 ctgaagcaaa tcaataaaaa cagaaagagt cacagcagag tatatactct taactccgaa
     1141 gcctactcca agcagccaga atgcaagtat aaaaagaaac atattgtaaa taagcatctg
     1201 ggtacctatc caccagttga aaagatagtt ggtaaccata gaaagtccgg atacaccgcc
     1261 ggcaattata ttgaacggaa ccaaaaaaac aactattccc aaagcagtaa aaaaagttcc
     1321 tgccgttatt ataatataat ccgcggcagt tcgtctgaca tcaacaggcc ttcttaattt
     1381 caaacccata tatccctccc gtctgtgcca aaaatacttc gtgcaagaag ctactcggcg
     1441 ctgattcccc gcatctgtgc aagctcctta agaagctttt tttcgcggat tgaaggctta
     1501 tctatcttta cgtttatcct cacgtaaata tccccgcgtt ttttagttct gaaatcaggt
     1561 attcctaagc ctttaagtct gaccacggta ttaggactgg ttccttcgga aattttttcg
     1621 tatacatcac cttcaagagt cggtatcttt atcctgtcac ctaaaagcgc ctgcagataa
     1681 tctatgtcaa cctgggtttc aagatctgtc cccttacgaa tgaacctttt atctggattt
     1741 actcttattc ccacaagcag atcaccgttt ggtccgccgt tctttccgga gtttcctttt
     1801 cctgttactc ttaatatgaa gccatcttca accccgcttg gaatagttat gacaaatttt
     1861 tcgtttttaa aggtctttcc ggcaccgctg cagacagaac actttttttc tactatcttc
     1921 gccgtgccgt tacaggtggg acaaggatat gtttttacaa acactccgaa aaaagttctc
     1981 tgttcttcat gtatagtacc ttcgccatta cacctcggac attttctgaa agatgtgcca
     2041 tgctccgaac catttccgtg acatgcggga caagcctcat acttattata ctctattgtt
     2101 ttctgagtat cctcaagtac ttctctcata tccatagtga cgttaactct tatatcctca
     2161 cctttctgag ggggctgaga agttcttctc tgacttttgg atgaagattc attgaaaaac
     2221 atatcaaaaa aatcagaaaa agggcctgct cctgccctgc cttcaccgaa tatatcctta
     2281 aagacatcct caaatcctcc ggaacctgaa taaccatcag aatttggcat tccgccgttt
     2341 tccggaacaa aaccgaatct gtcatatagc tttttctttt caggatcgct taaaacctca
     2401 tatgcttcct gaatctcttt aaacttttct tcagcaacct tcttatcctc aaagtgcctg
     2461 tcgggatgcc attccttgac aagaagccgg tacgccttct ttatatcgtc aagcgaagca
     2521 tctcttgaca ccccgagcgt accgtaataa tcttttttct gaggcattat ttttcacctt
     2581 cttcagattt ttccgtacac tccgttttaa cttcctcttt cttctctttg ggtttaactg
     2641 ccacaacaac ctgtgcagca cgtaaaaccg taccgttaag cttgtagccg taggttttgg
     2701 tttcaaagac gtgatactca cttacatcag aggtttcaca cttatcaatt acttcatgct
     2761 caaaaggatc aaactgatct gatttttcag gatgaataaa cgttaatcca catttttcaa
     2821 aggcgttaat aaaatctctg aatatcatct ccatagcttt ggcaaaatcc gaaccatcgg
     2881 aatacttcat tgatatacta aacttttcaa agacaggtat aaactttttc aaagtatcct
     2941 ctttggtttt tatctctata ttcttctttt ctctgtccac tatatccttg aaactttcaa
     3001 aatccatttt caattgtata gcagcgtttc tgaacttcat tacttcctgc tcaagttcgg
     3061 agatcctttc cttgcttttt tcaagctccg catactcggg tgtattgttc tgacattctt
     3121 tcttttcagc agtttcacct acgcttgatt gctcatttat aacttgttcc tgctcttttt
     3181 ttatgtcctg agacatactg cacctccaga tctatttttt aaagattttt agatattatc
     3241 tcggaaaatc ttgatgtata ttcattaata actttataat ttttttcgta gtcagaaaat
     3301 ttatttgtaa taacaaagat cctgcctaca ggattcacac cattacagta tgaagtatag
     3361 aaaaaagcaa aattctttaa tggcctgtat gtttcccctg tcaccagctc gtttccaaga
     3421 caaagattga tatccctgtt ggcggtcaga ttcctgaaaa gttcttcctt tatcttatcg
     3481 ttacccacaa tacatgcaag catctcgatg ctttctatgg gcggccggtt tttagatatc
     3541 agaatatcaa agccgtttgt agtatacttg attctggaga attcatttac aagaccttct
     3601 accagtttga acatgtttat cactctctca tcctgagcct ggacagactg caattttaca
     3661 gccgagaata cctccgagag agatttacct gtaagtcctt tattcaaaag gctttccagt
     3721 tctttaggat gaggaagatc gtatatctcc attgtacgcg aaattgtaag tccaagactg
     3781 gtaagcaggg ttattacgca atgactttca gtcaccggag ttacaaccgc tctggttata
     3841 taaagatacc tcggatttgg atactcaaat attacaagcg attcaagggt attggatatc
     3901 attttggaaa cattttcaaa catcaggttg agatcataaa acttatactc ttttggaata
     3961 tcaagaacgg atgaactgtt cgaataaagt tccttgacca catcgaagta gagtttgacg
     4021 gcacggtcgg taggaattct tcctccgctt gtatgttgct gaaatatata cttcaggtgc
     4081 tggagctttt gcatgtcatt cctaaccgta gcactgctgg tattcattct ggtattgctc
     4141 agtacatcat cagagctcac cggtttttga tttttgatat aaagcgttac tatggccatc
     4201 agaacatctt tttgcctctc tgttaagtca gggcccattt ttatcactcc tcttttttga
     4261 gtgctaatag tattatagca aaaactttta tttttgccaa ataaaaaggt taattattga
     4321 ttaaattatc ttaatactct ccgaatcacg attgaagata aaaaacttgt caaagtcaaa
     4381 aaatagatgt aaaattgaaa aggtgcggaa aattaaagat ttttgggagg gaatgactta
     4441 tgattactct taatcaggta atagagtgtc ttaaaggcca gactgtaaag aaaagagtac
     4501 ccgaacagtt agaacgagag ttatacggtc tgacatccaa cagcaaggaa acaaggaaag
     4561 gcgatatttt tgtagcagtt aaaggaccaa aagtcgacgg ccatgattat atagacatag
     4621 cagttaaaca cgaagccggt cttgtcattc ttcagaacaa atcaaaaatt ccgcctgaac
     4681 ttcagtttga ctatatcttg gtaaaggata ccagaaaagc tctcgcggat attgcatatc
     4741 tcatggctga tgtagatcca aaagatttca gattttttac cgtgacaggt accaacggaa
     4801 agtctacctg tgtatgtttg cttcatcatc ttatgaggga atctcacagg gattcggttc
     4861 ttatgagcac cgtcgaaata aaagtgaatg acgagcttat agacgaacca tacaacacta
     4921 ctccgggagt gctggaaata gcagatgtac ttaagaaggc aaaggaaaac aaaattgatt
     4981 ttataaacct ggagatatct tcccacggta tagcacaaaa aagaatcgag aatcttaaat
     5041 tcgatgtgat ctcatttact aatatcacaa gagatcatct cgattaccat aaagactttg
     5101 aagagtacga agcaaccaag ctttctctgg tggattacct taaacctaac ggaatcgcgt
     5161 ctgtaaacat agatgagata gatgtaaata agatacgtct tccgtccgaa cgcattataa
     5221 cttacggaca gtccgagaaa gcagattaca gaataaagga catcaattgc aatatatatc
     5281 agatgaactt caaaatacaa accccacagg gaaacgatat ctctgtatat acatccataa
     5341 tcggtgagtt taatgcctac aacataacta atacggtaat cgccgcaaag tacttcggac
     5401 ttactgacga ggagataaaa catggaatca taactttcaa aggtgtcccg ggaagatttc
     5461 agcttgtacc caccagcaaa tctctgggat tttcagttgt ggttgatttt gcacatactc
     5521 cggatgcact ggaaaaagtg ctgaaaaccg ccagaatggt ttcaaaaggc cgtgtaataa
     5581 ttgttttcgg agcaggaggc aacgccgata tcggtaaaag gcttatgatg ggtgaagtaa
     5641 caagcagata tgccgatata gtcgttataa ccaatgatga tccaaaggac gaagaaccgg
     5701 aaaagataat tgaagacatt aaacaggggc tggatgtaag aaagaacttc attgtcatcc
     5761 ctgacagaaa aacggccata gatgcagcca taaactttgc taacagggac gatatagttg
     5821 ttattgccgg aagagggcat gaaaaatttc aattgttttc aaacggtaaa aagatcaaat
     5881 tcaatgatta cgaggttgcg ggagagataa tagaaaatct caagaggagc atgaaaaaat
     5941 gag
//
LOCUS       DAOSHR010000127         5937 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_11805_length_5937_cov_5.825740, whole genome shotgun sequence.
ACCESSION   DAOSHR010000127 DAOSHR010000000
VERSION     DAOSHR010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 5937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5937
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11805_length_5937_cov_5.825740"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2192
                     /locus_tag="PLU28_07575"
     CDS             <1..2192
                     /locus_tag="PLU28_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34958.1"
                     /translation="SAFSKIDINLSHLEFLKDSFNIEGKYYTGYWIYADKTAGAYKHA
                     PAVSEGVTCVDDVARVAILYTDLYRQKSNSLYMQRAREALEFVLAMQDNDGDFYNFID
                     ENGTINRTGITSRKSKSWWAARAFWSISNGMEIFKSTDSQFYDRLSVAAKSSFDLLKG
                     SLDKSFLINGYTDVSSVFLLGLVKYYQNSPDEKTLELAQSIGKAIIDKQLDESAYKGV
                     YNESNSGFAWHSWGSRQAQALADLYTICGKEEYIASARKYADNWLEVLLSLGPIYEVK
                     EYFALYPQIAYGAEAASSSCSSIFRATGEERYGYLAALFAGFFFRNNILSVPMYGKNG
                     EGYDGLHSVYINSNAGAESTISALLTLMQINSLDEKFHRFIDAKVIGASKTILMEAEK
                     FDPGISSFDIDNSGNVRLASKEKLVIKSSVPLEKDIYDVYMLGSVQASDVAVKTYFGE
                     NIQETVLKRGSVIYSLAQGISYSSGKVTLSFTPSNGYVYADQILFVPQVHKFVIELDG
                     EFFLFSGGRMISTDYTLSAQSSKAYTDKFTVKTFENKGHINVDLSEVFNNDGIVDFKN
                     RREGNFDNPEGVFGAKYSQEEIEKKKAEGYILSGTVPFFIQTTTKDNIVCTGQELLFD
                     KPVYAKYMYIAGSCDHGSYESKLTIQYEDKSSSEVDLKFSDWCQEPIYGESTLLNGKY
                     RYTSLGIKENINPKIFIQKIELSPKGTERIYLPKSPTMHIFALTFSY"
     gene            2299..2841
                     /locus_tag="PLU28_07580"
     CDS             2299..2841
                     /locus_tag="PLU28_07580"
                     /inference="COORDINATES: protein motif:HMM:NF022636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HOG34959.1"
                     /translation="MINESFKKLSSFLSGFFSVCFYISIAAAVFLLGMGFFWMSKDRF
                     INFRIDYYGLSFNINEMVFSKELFASYILGTAVLILTIFWLKKIFSNIKKEKIFVKEN
                     FSLMSKLSVNIMIMSVIQGTIDFFNGRVIAEKFIKEGSDYFVNFRININLLILAVIIF
                     VFAQILKMAADMKEENDLTI"
     gene            2857..3078
                     /locus_tag="PLU28_07585"
     CDS             2857..3078
                     /locus_tag="PLU28_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOG34960.1"
                     /translation="MAIVVNLDVVMAKKKKNSKDLAAALDITQANLSVLKTSKAKAIR
                     FSTLEGICRFLDCQPGDILEYVPEKPSDQ"
     gene            complement(3072..3296)
                     /locus_tag="PLU28_07590"
     CDS             complement(3072..3296)
                     /locus_tag="PLU28_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34961.1"
                     /translation="MGKSKEEFLQGNVFSFRNTKDMKVFIYFKNKEARILKGEQAQKF
                     LCDISAASPSEQQLIMAKITGNFKHGNESH"
     gene            3392..4210
                     /locus_tag="PLU28_07595"
     CDS             3392..4210
                     /locus_tag="PLU28_07595"
                     /inference="COORDINATES: protein motif:HMM:NF022431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
                     /protein_id="HOG34962.1"
                     /translation="MDTILQLKNIKENNFKPFENTDIDDLTLFMLREIGNLDPYLRDT
                     LINDVFSMFIENGVYQDRKLEEILNVLLDQEHLFFNIGHEDDSSVFKRSFSMLIIAEI
                     LRVNTKNNFLSQEVLKIVQQDFISYYNMERDLRGYVKDAGWAHSVAHAADCLKMIVHS
                     PYVEKDFVNSALKGIITKFCIHNYVYINEEDERTVSAVVAMYQNGHVSDQELIDWLND
                     FEKAMYITAFPQRHYLLTNVKGLLRSMYFRFSKYSLSPEVMESIRQVLIKNSKF"
     gene            complement(4346..>5937)
                     /locus_tag="PLU28_07600"
     CDS             complement(4346..>5937)
                     /locus_tag="PLU28_07600"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOG34963.1"
                     /translation="YDAIAYCNWLSEKEGLAKAYDETGTLIDSKGKITTDPSLVEGYR
                     LPTEAEWEYAARGGNKSKGYKYSGSNKADDVAWYEKNSGGTTHEVGTKQPNELGIYDM
                     SGNIDEWCIDIYKWNIYWKDEHNYKNVPTVNQCYTDCVNDNFDRYELILRGSCYKTSE
                     FYLPLFFRDGEYPYDVGEYYGFRLARTEPHKTIWEKDSNTKLKIDASQEDAQVYLNGK
                     YIGTAPVSIDTKCMHNAQIKVVKEGYEEKIIKNVYIPKNETVQVYAELKKKEPQMVLV
                     EKGSFDMGGKDDSRDWLRKYAHKVTLTYDFYIGKYEVTHDQYYRYCEETRREMRLEYF
                     LIRKSNYPAAQVSWYDAIGYCNWLSEKEGLAKAYDEKGNFLDKNGNITTDPDRTEGYR
                     LPTEAEWEYAAKGGNKSNGYKYSGSNNPDDVAWYGEFFITGTAHEVGTKQPNELGIYD
                     MSGNVSEWCNDEDSYYTSSSDQINPYVFPKVTSEKKIFRITRGGDWHSDANIILNYYR
                     WNMYSARSDSYTGFRIVRVKP"
BASE COUNT         1733 a         1104 c         1160 g         1940 t
ORIGIN      
        1 tatcagcttt ttcaaagata gatataaatc tttcgcatct ggagttttta aaagatagtt
       61 tcaatataga ggggaagtat tacaccggct attggatata tgccgataaa acagccggtg
      121 catataagca tgctcctgcg gtatctgaag gggttacctg tgttgatgat gttgcaagag
      181 tggcgattct ttatacagat ttatacagac agaaaagtaa ctccctttat atgcaaaggg
      241 caagagaagc tcttgagttt gttcttgcta tgcaggacaa tgacggagat ttttataatt
      301 ttatagatga aaacggtaca attaaccgta ccggaattac aagcagaaag agcaaaagct
      361 ggtgggcagc gagagctttc tggtctattt ccaatggtat ggaaattttt aaaagtactg
      421 acagccagtt ttacgataga ctttctgttg ctgcaaaaag ttcttttgat cttttaaagg
      481 gtagtcttga caaaagtttt ctaattaacg gatatacaga tgtaagttca gtttttcttc
      541 ttggtcttgt gaagtattat cagaattccc cagacgaaaa aacacttgaa cttgcacaga
      601 gcataggaaa agccataatc gacaaacagt tggatgaatc ggcctataaa ggagtatata
      661 atgaatcaaa ctccgggttt gcatggcata gctggggaag ccgtcaagca caggctcttg
      721 cggatcttta tacgatttgc ggaaaggaag agtatattgc ttcggcaaga aaatatgctg
      781 ataactggct ggaggtactt ctctcacttg ggcctatcta tgaagtgaag gagtattttg
      841 cgctttatcc tcagattgcg tacggagccg aagcggcttc ttcttcgtgt tcaagtattt
      901 tccgtgcgac cggtgaggaa aggtacggct atcttgcagc actttttgca ggatttttct
      961 tcagaaacaa tattctttcc gttcctatgt acggtaaaaa cggcgaaggt tatgacggtc
     1021 tgcactctgt ctatatcaat tctaatgcag gtgctgagtc tacaatatcg gcacttttaa
     1081 cacttatgca gattaattcg ctggatgaaa aatttcatag gtttatcgat gccaaagtaa
     1141 taggagcgag caaaactatt ctgatggaag cagagaagtt cgatccaggc atatcttctt
     1201 ttgatataga taactccgga aatgtccgac ttgcatcaaa agagaagctt gtcattaaaa
     1261 gctccgttcc tcttgaaaag gatatatacg atgtttatat gcttggttct gttcaggcat
     1321 cggatgttgc ggtgaaaacg tattttggag aaaatattca ggaaactgta ttaaaaagag
     1381 gttctgtaat atacagtctt gcacagggta tttcctattc ttccggaaag gttactcttt
     1441 cgtttacgcc ttcaaatggg tatgtttatg ctgatcagat actttttgtt cctcaggtgc
     1501 ataagtttgt tatagaactt gacggagagt tctttctttt cagtggcgga agaatgatta
     1561 gtacggacta cacactttca gcacagagca gtaaagcata taccgataaa tttactgtaa
     1621 aaacttttga aaataaaggt catataaatg tcgatctttc ggaggtcttc aataatgacg
     1681 gcatagttga ttttaaaaac cggcgggaag gcaattttga taatccggaa ggggtatttg
     1741 gtgccaagta ctcgcaggaa gagattgaaa agaaaaaagc agaaggttat atactcagcg
     1801 gaactgtgcc gttttttatt cagacaacca caaaggataa tattgtatgc accggacagg
     1861 agcttctttt tgataaacct gtctatgcaa agtatatgta tatagccggt tcttgtgatc
     1921 atggaagcta tgaatcaaaa ttgaccatac agtatgagga taaaagcagt tcggaggttg
     1981 atctgaagtt ttcagactgg tgccaggagc caatatacgg tgaaagcact cttctgaatg
     2041 gaaaataccg ttatacgagc cttggaataa aagaaaacat aaatccaaag atatttatac
     2101 agaagataga actctctcca aaaggaactg aacggatata tctgcctaaa agccctacca
     2161 tgcatatttt tgcactaacc ttttcttatt agtgttgtat aacaacaaat aattattgtt
     2221 tgtcaataaa aaatagttga caaacaataa ttttttattg tataatagta aagaaaatta
     2281 aaagtatgga ggtatactat gataaacgaa agctttaaaa agctaagctc atttctatcc
     2341 ggttttttca gtgtatgttt ttacatttcg atagcagcgg cagtttttct tttagggatg
     2401 ggatttttct ggatgagcaa agacagattt atcaacttca ggattgatta ttacggtctt
     2461 tcatttaata taaacgaaat ggtgttttca aaggagcttt tcgcaagtta tattcttgga
     2521 acggcagtac ttattctgac aatattctgg cttaaaaaaa ttttttccaa tataaaaaag
     2581 gaaaagatct ttgtcaaaga gaacttctca cttatgtcaa agctttctgt aaacattatg
     2641 attatgtctg tgattcaagg aacgatagat ttcttcaacg gcagagtaat tgcagaaaag
     2701 tttataaaag aaggcagcga ttattttgtt aacttcagaa tcaatattaa tcttcttatt
     2761 ctggcagtaa taatttttgt ttttgcccag attcttaaga tggcagcgga tatgaaagaa
     2821 gaaaatgacc ttacaatata ggacggggag gatataatgg ctatagtagt taatcttgat
     2881 gtggttatgg caaaaaagaa aaaaaactcc aaggatcttg ccgcggcgct tgatattacc
     2941 caggcaaatc tttccgtgct taaaacatca aaagcaaaag ccataagatt ttcaactctg
     3001 gaaggaatat gcagatttct tgactgtcag cctggagata tacttgagta tgttccggaa
     3061 aaaccaagcg atcaatgact ttcatttcca tgcttaaaat ttcctgttat tttagccatt
     3121 ataagctgtt gttctgaagg agatgcagcg gagatatcac aaagaaactt ttgtgcctgt
     3181 tctcccttaa gtattctggc ttctttattc ttgaagtaga taaaaacctt catatccttt
     3241 gtgtttctga atgaaaaaac attgccttga agaaactcct ctttgctttt gcccattttt
     3301 agtgcctctt ctttatagta tttatcaaat atatgatatt attataatac aatatgagta
     3361 catttactat taagaaaagg gggaaaaacc aatggatacc attcttcagc ttaaaaatat
     3421 aaaagaaaac aactttaagc cgtttgaaaa taccgatatt gatgacctta cactgtttat
     3481 gctcagagaa atagggaatc ttgaccctta cctgcgcgat acactgataa atgacgtatt
     3541 ttcaatgttt atagaaaacg gagtttatca ggacaggaag cttgaggaga ttcttaatgt
     3601 tcttcttgat caggagcatc tgttttttaa cataggacat gaagacgaca gttctgtgtt
     3661 caaacgttcg ttttccatgc ttataatagc cgagatactc cgagttaata caaaaaacaa
     3721 cttcctttcg caggaagtat tgaagatcgt tcagcaggat ttcatatcgt actataatat
     3781 ggaaagagat ttgagaggat atgtaaaaga tgccggatgg gctcattccg tggcacatgc
     3841 cgccgactgt ttaaagatga ttgtgcatag tccttatgta gaaaaagatt ttgtcaactc
     3901 tgcacttaag ggcataatca caaagttctg catccataac tatgtgtata taaatgaaga
     3961 agatgaaaga acagtaagtg ctgtagttgc catgtaccag aacggacacg tgtccgatca
     4021 ggagctaatt gactggttga atgattttga aaaggcaatg tacataacag catttcctca
     4081 aagacattat cttcttacaa acgttaaagg gctgttaagg tcaatgtact tcagattttc
     4141 aaagtatagt ctttctcctg aggttatgga gtcgatacga caggtgctta taaaaaattc
     4201 aaaattttaa agattaagtc ccttgatatt tttcaaggga cttttgttcg gtctttcgtc
     4261 tctgttattg agcttttaac taaacaataa aagtcttatg cttaagcgca aagtaaacag
     4321 ggacatcgaa tgatgtccct gttttttatg gttttacccg tacaatccta aaaccagtat
     4381 aactgtcgct tctcgcggag tacatgttcc acctgtaata gtttagaata atgttagcat
     4441 cagagtgcca gtctcctccg cgtgtaatcc gaaaaatttt tttttctgaa gtaactttag
     4501 gaaacacata cgggttaatc tgatcagatg acgatgtata gtatgaatcc tcgtcgttac
     4561 accactcgct cacattgcct gacatgtcgt atattccaag ttcatttggc tgtttggttc
     4621 ccacttcgtg tgcagtaccg gtaatgaaga attctccata ccatgccaca tcatccggat
     4681 tattgctccc gctgtactta tacccgttgc ttttgtttcc gcccttggct gcatactccc
     4741 actctgcttc tgtaggaagg cggtatcctt ctgttctgtc cggatcggtg gttatattcc
     4801 cgtttttatc cagaaagttt cccttttcat cgtatgcttt tgccaaccct tctttttcac
     4861 tcagccagtt gcagtagcct atggcatcat accaactcac ctgtgcagca ggataattac
     4921 tttttctgat taagaaatat tctaggcgca tctctcttct tgtctcttca cagtatctgt
     4981 aatactgatc atgcgttact tcatactttc ctatataaaa gtcatatgtc agtgtcactt
     5041 tgtgtgcata tttccgcagc caatctctgc tgtcatcctt accgcccata tcaaagctcc
     5101 ctttttctac cagtaccatc tgtggttctt tcttcttaag ctctgcataa acctggacag
     5161 tttcattctt gggtatgtat acatttttta tgattttttc ctcgtatcct tctttgacta
     5221 cttttatctg tgcgttatgc atgcatttcg tatctatact cacaggagct gtgcctatgt
     5281 actttccgtt cagatacacc tgtgcatctt cctgacttgc atctattttc agttttgtat
     5341 tactgtcttt ctcccatatt gttttatgtg gctctgtcct tgcaagacga aacccatagt
     5401 attcgccgac atcgtaggga tattctccgt cacgaaagaa aagaggcaaa taaaactccg
     5461 aagttttata gcaactgccg cgtagtatca gttcataacg atcaaagtta tcattgacac
     5521 agtccgtata gcactggttc acggtcggaa cattcttata gttgtgttcg tctttccaat
     5581 atatattcca cttgtatata tctatacacc actcgtctat gtttccgctc atatcgtata
     5641 ttccaagctc gtttggctgt ttggttccta cttcgtgagt agtgccgccg gaattttttt
     5701 cataccatgc cacatcatct gctttattgc tgccgctgta cttatatccc ttgcttttgt
     5761 ttcctcccct tgctgcatac tcccactctg cttctgtagg aagacggtat ccttcaacca
     5821 ggcttgggtc tgtggttatc tttccttttg agtcaatgag ggttccggtt tcatcgtatg
     5881 cttttgccaa cccttctttt tcactcagcc agttacagta ggctatggca tcgtacc
//
LOCUS       DAOSHR010000128         5910 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_11875_length_5910_cov_6.272246, whole genome shotgun sequence.
ACCESSION   DAOSHR010000128 DAOSHR010000000
VERSION     DAOSHR010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 5910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5910
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11875_length_5910_cov_6.272246"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..440)
                     /gene="rpsH"
                     /locus_tag="PLU28_07605"
     CDS             complement(36..440)
                     /gene="rpsH"
                     /locus_tag="PLU28_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOG34964.1"
                     /translation="MWSDPIADMLTRIRNANSVEKESVEIPASNLKKAILDILKLEGY
                     ISDYKVIEDGKQGVLKVTMKYKGDRRNTQKVITGIVRVSKTGRRIYVKAEEIPLVKGG
                     MGIAIVSTSKGVLTDKQAREIGVGGELLCYVW"
     gene            complement(455..640)
                     /locus_tag="PLU28_07610"
     CDS             complement(455..640)
                     /locus_tag="PLU28_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HOG34965.1"
                     /translation="MARKGLVERMKTEPKFKTREYTRCLVCGRPRSVYKEFGLCRVCF
                     RKMALEGKLPGVKKASW"
     gene            complement(657..1208)
                     /gene="rplE"
                     /locus_tag="PLU28_07615"
     CDS             complement(657..1208)
                     /gene="rplE"
                     /locus_tag="PLU28_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOG34966.1"
                     /translation="MAEFIPLKEKYNSEAVAALKKEFNYTNPHEVPKIVKIVVNMGVS
                     EGSRNYEVLEKHANELAAIVGQKPVITKAKKSISNFKLREGMPIGAKVTLRGATMYNF
                     LYKLVNIVLPKLRDFRGLPVNSFDGRGNYTFGLTEQIVFPEIRPDQAKRVQGMDITIV
                     TTAKTDVEAKKLLEFVGFPFKKN"
     gene            complement(1225..1542)
                     /gene="rplX"
                     /locus_tag="PLU28_07620"
     CDS             complement(1225..1542)
                     /gene="rplX"
                     /locus_tag="PLU28_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOG34967.1"
                     /translation="MNIKKDDLVQIISGKDKGKKGKVLKVINKYSKVVVENVNVVKKH
                     QKPNQQMKEGGIIEQPSPLYACKVMVVCSSCGKPTRIGYKLLDDNKKARYCKKCGEII
                     DRV"
     gene            complement(1557..1925)
                     /gene="rplN"
                     /locus_tag="PLU28_07625"
     CDS             complement(1557..1925)
                     /gene="rplN"
                     /locus_tag="PLU28_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOG34968.1"
                     /translation="MIQLESYLRVADNSGAKVIKVIKVLGGFHKSTGSIGDVVVCSVR
                     EAIPHTDIKKGQIVKAIVVRTHKEIGRKDGTYIRFDDNAAVLIDKQNLPLGTRVFGPI
                     AREVREKGYAKIASLAAEVW"
     gene            complement(1922..2224)
                     /gene="rpsQ"
                     /locus_tag="PLU28_07630"
     CDS             complement(1922..2224)
                     /gene="rpsQ"
                     /locus_tag="PLU28_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOG34969.1"
                     /translation="MPKKVLVGKVLSDKMDKTVTVEVKSRFKHPVYNKIGEKTKKYHA
                     HDENNVCKVGDVVEIMESKPISKTKKWVVSKIVEENIFKSNETPETVEQILGGEEK"
     gene            complement(2229..2429)
                     /gene="rpmC"
                     /locus_tag="PLU28_07635"
     CDS             complement(2229..2429)
                     /gene="rpmC"
                     /locus_tag="PLU28_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOG34970.1"
                     /translation="MNTAEFKTLTEQELNLKFEETKKKLFDLRFQLEMGQLKNTATIK
                     QVKRDIARIKTILRERELGIRR"
     gene            complement(2429..2854)
                     /gene="rplP"
                     /locus_tag="PLU28_07640"
     CDS             complement(2429..2854)
                     /gene="rplP"
                     /locus_tag="PLU28_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018133712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOG34971.1"
                     /translation="MLMPKRVKYRKVQRGRMCGESKGAFQVDFGEWGLKALEPAWITS
                     QQIEACRLAMVRTLKRTGKVWMRVFPDKSYTTKGIGTRQGVGKGDIEGWAAVVKPGRV
                     MFEIGGIDEATAKEALQYAASKLPIKTKIVPRYQIGGEQ"
     gene            complement(2875..3507)
                     /gene="rpsC"
                     /locus_tag="PLU28_07645"
     CDS             complement(2875..3507)
                     /gene="rpsC"
                     /locus_tag="PLU28_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOG34972.1"
                     /translation="MGSKVNPLGFRVGVSKPWKSEWFNEKNYKEYLHEDKKIREYIKN
                     LYKASGIADIDIERPSDTLVKITIHASRVGIIIGKKGEEIASLREKIKTIINDRTVKV
                     YVEEVKNPSLNAQLVADDIKEQILRRVSHKVAMKRAITNSMKRGAKGIKIMVSGRLGG
                     AEIARTEWYMEGRLPLQSLKSDIDYSIVDAHTKYGTIGVKVWIYKGDVQI"
     gene            complement(3524..3958)
                     /gene="rplV"
                     /locus_tag="PLU28_07650"
     CDS             complement(3524..3958)
                     /gene="rplV"
                     /locus_tag="PLU28_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOG34973.1"
                     /translation="MALVSRVQDGKRVKKSVYHRLRKEQEATQPKVQASATAKFLRMS
                     ATKARAAANGVRGKDVGQALQTLMFSPKKSSRFIYKLLMSAIANAENNLGLKSDNLYV
                     SEIMVNEGPRMKRLWARSRGSGDIQQKRMCHVTLTVKDKNAK"
     gene            complement(3973..4266)
                     /gene="rpsS"
                     /locus_tag="PLU28_07655"
     CDS             complement(3973..4266)
                     /gene="rpsS"
                     /locus_tag="PLU28_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOG34974.1"
                     /translation="MSRSLKKGPYVHPSLLKKIREMNSKGEKKVVKTWSRASMIIPEM
                     VGHTIAVHNGMKHIPVYISEQMIGHKLGEFSPTRRFGGHPDKKAASATKGKVK"
     gene            complement(4283..5113)
                     /gene="rplB"
                     /locus_tag="PLU28_07660"
     CDS             complement(4283..5113)
                     /gene="rplB"
                     /locus_tag="PLU28_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOG34975.1"
                     /translation="MGLKKFKPTTPSRRFMIINDFAEITTDKPEKSLLAPLKSNGGRN
                     HHGRITVRHQGGGHKRRYRIIDFKRDKYDIPCKIASIEYDPNRSARIALLVYTDGEKR
                     YILAPKGVKVGQELLSSPKAEIKPGNALPLANIPLGTLVHNIEFEPNRGGKIARSAGV
                     YAQLMAKESGYALLKMPSGELRRVRETCYATVGMIGNEDHVNEVHGKAGRRRWLGIRP
                     TVRGMTMNPVDHPMGGGEGKCKGHIPQSPWGMPAKGYKTRRGKRRTDRLIVRRRNENK
                     "
     gene            complement(5127..5426)
                     /gene="rplW"
                     /locus_tag="PLU28_07665"
     CDS             complement(5127..5426)
                     /gene="rplW"
                     /locus_tag="PLU28_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOG34976.1"
                     /translation="MENNKLNYDIVLKPILTEKSYQLISESKYTFEVAKDATKTEVKT
                     AIEKLFNVKVEKVNIMNVKSKPKKMGRFEGYTRGWKKAIVTLAQGYVIKELQGNV"
     gene            complement(5416..>5910)
                     /gene="rplD"
                     /locus_tag="PLU28_07670"
     CDS             complement(5416..>5910)
                     /gene="rplD"
                     /locus_tag="PLU28_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOG34977.1"
                     /translation="GRKPWAQKRKGRARAGSSRSPIWRHGGVTFGPKPRDYDKSLNKK
                     MKKLALKSALSVRLRENNIVVIDDIKFDTPRTKQMKEVIEKISLTGKKVLFVLPFKNE
                     EYHNVKLSANNIPKVKVIIADNPNQGKMNIDGLNVFDLINNEKIVLTKELVKKIEEVL
                     DNGK"
BASE COUNT         1345 a         1426 c          942 g         2197 t
ORIGIN      
        1 gcaactcttg attttggcat ttgcagtcac cctccttacc atacatagca gagcagttct
       61 ccaccgacgc ctatttctct ggcttgttta tcggtaagaa caccctttga tgttgaaacg
      121 atggctatac ccattccgcc tttaaccaaa ggtatttcct ctgctttaac gtatattctt
      181 cttcctgtct tggagaccct tactattcct gtaattacct tctgagtatt tctcctgtca
      241 cctttgtact tcatggttac ttttaatact ccctgtttgc cgtcttcaat caccttgtaa
      301 tctgaaatat acccttcaag tttcaatatg tcgagaatgg ctttctttaa atttgaagca
      361 gggatttcaa ccgattcttt ttcaaccgaa ttggcatttc ttattcttgt aagcatatct
      421 gctattggat cactccacat cgttgcttcc ctccttacca gcttgctttt ttaactcctg
      481 gaagttttcc ctccagggcc atctttctga agcacactct gcaaagtcca aattctttat
      541 aaactgatct tggtcttcca cagactaagc atcttgtata ttctctagtt ttaaacttag
      601 gttctgtttt cattctttca actaagcctt ttcgtgccat ttatcaatcc ctccattcag
      661 ttcttcttaa atgggaagcc aacaaattcc agaagtttct tagcttctac atcagtcttg
      721 gctgtggtta ctatggttat atccataccc tgaacccttt ttgcctgatc aggtctgatt
      781 tcaggaaaca ctatctgttc tgtcaaaccg aaagtatagt tgcctcttcc gtcaaacgaa
      841 ttaacaggta aacccctgaa gtctctcaac ttaggaagaa ctatgtttac aagtttgtat
      901 aaaaagttat acatggttgc tcctctgaga gttaccttgg cacctatggg cataccttcc
      961 ctgagcttga agttggatat gcttttcttt gccttggtta taacaggttt ctgtccgact
     1021 atggcagcaa gttcattggc atgtttctca agaacttcat agtttcttga tccttcactt
     1081 actcccatgt taacaactat tttaactatc ttaggaactt catgaggatt tgtatagtta
     1141 aattctttct ttaaagcggc aacagcttca ctgttgtact tttcctttaa tggtataaat
     1201 tcagccattt ctattcctcc cgacttatac tctatcaatt atctcgccac attttttaca
     1261 gtaacgagct tttttgttgt catccaatag tttgtatcca attctcgtgg gttttccaca
     1321 gcttgagcaa actaccatta ccttgcaggc atataatggg gatggctgtt caataattcc
     1381 gccttctttc atctgctggt taggcttctg atgtttctta acaacattta cattctctac
     1441 aaccactttg ctgtacttat tgattacctt cagaactttt ccctttttac ccttatcttt
     1501 tcctgagatt atctgaacta aatcgtcttt ttttatgttc atgcctgtca cctcaatcac
     1561 catacttccg ctgctaagga agcaatcttg gcataaccct tttcccttac ttcccttgcg
     1621 ataggaccaa agactcttgt acctaacgga agattctgtt tatcgatcaa tactgcagca
     1681 ttatcatcaa acctgatata tgtgccgtct tttctgccaa tctctttatg tgtcctaaca
     1741 actatggctt taactatctg tcctttcttt atatctgtat ggggaatagc ttcccttacc
     1801 gaacagacca caacatcacc aatgctaccg gtagatttgt gaaaaccgcc aagtactttt
     1861 ataactttaa ttaccttggc tcccgaatta tcggctactc tgagatacga ttcaagctgt
     1921 atcatttctc ttcaccccca agtatttgtt ccacagtctc gggtgtctca tttgatttga
     1981 aaatattttc ttcaacaatc ttgctgacta cccatttttt ggttttggaa atgggctttg
     2041 attccattat ttctactacg tcccctactt tgcagacatt attctcatca tgggcatggt
     2101 atttttttgt tttttcgcct atcttgttgt agacaggatg cttaaatctg ctttttactt
     2161 caactgtaac tgttttatcc attttatcgc ttaagacctt tcctactaaa acctttttcg
     2221 gcatactcct acctccttat acccagttct ctttctctga gtatagtctt gatccttgct
     2281 atgtctctct tcacctgttt aatggtagca gtgttcttca actgtcccat ttcaagctga
     2341 aatctaagat caaacagttt cttctttgtt tcctcaaatt tcagattaag ttcctgctct
     2401 gtcaaagtct tgaattctgc agtattcatt actgttcacc ccctatttga tacctgggaa
     2461 ctatcttggt ctttatggga agtttgcttg cagcatactg caatgcttct ttagcagtgg
     2521 cttcatctat tccgccaatt tcaaacatta ctcttcccgg ctttaccact gctgcccagc
     2581 cttcgatgtc acctttaccg actccctgtc ttgttcctat acctttggtc gtataagact
     2641 tatcagggaa tactctcatc caaaccttac ctgttctctt aagtgttctt accattgcaa
     2701 gtctgcatgc ttctatctgt tgggaagtaa tccaggccgg ctcaagtgcc ttaagccccc
     2761 attcgccgaa atcaacctga aaagcccctt tagactcgcc gcacattctg ccgcgctgaa
     2821 cttttctata tttaactctc ttaggcatta acattttcta gctacctccc tatattatat
     2881 ctggacatcg cctttatata tccatacttt tacaccgatg gtaccgtact tggtgtgagc
     2941 atcaactatt gagtagtcga tatcggattt aagagactgt agaggcaatc ttccttccat
     3001 ataccattcg gttcttgcta tttcagcgcc gcccaatctt ccggaaacca ttattttgat
     3061 tcctttagct cctcttttca tggagttggt aatggctctt ttcatggcaa ccttatggga
     3121 tactcttcgg aggatctgtt cctttatgtc atcagctaca agctgagcat taagcgaagg
     3181 attctttact tcctcaacat acacctttac tgttctgtca ttaataatag tttttatctt
     3241 ttctctgagc gaggcgatct cctcaccttt cttacctatt attattccga ctctggaagc
     3301 atgaatagtt atcttgacaa gggtgtcgct aggtctttcg atatctatat ctgctatccc
     3361 cgaggcttta taaagattct ttatatactc tctgattttt ttatcttcat ggagatattc
     3421 tttataattc ttttcgttaa accattctga tttccaaggc ttagaaactc caactctaaa
     3481 acctaacgga ttaacttttg aacccacgga ttattcacct ctcttattta gcatttttat
     3541 ctttaaccgt taaggtaacg tgacacattc ttttctgctg aatatctccg cttcctcttg
     3601 atcttgccca aagccttttc attctgggac cctcgttgac cattatctct gaaacataaa
     3661 ggttgtctga tttgagtcca agattgtttt ctgcgttagc aattgccgac ataagaagct
     3721 tgtaaataaa tcttgaggat ttcttgggac tgaacataag agtctgtaat gcctgaccca
     3781 catcttttcc tcttactcca tttgctgcgg ctcttgcttt agtagctgac attctaagaa
     3841 acttagctgt cgcagatgcc tggactttag gctgagtagc ttcctgttct ttcctgagtc
     3901 tatgataaac ggatttcttt actctttttc catcctgtac tctacttacc aaagccataa
     3961 tttactccct cctcatttca cttttccctt agtagctgag gcagcctttt tgtctggatg
     4021 ccctccaaat ctcctagtag gggaaaattc acctaatttg tgtccaatca tctgttctga
     4081 tatatatacc ggtatatgct tcatgccatt atgaacggct attgtatgac ctaccatttc
     4141 aggaatgatc attgaggctc ttgaccaagt ttttacaact ttcttctcac ccttgctgtt
     4201 catttctctt atctttttca acaggctcgg gtgtacatat ggacctttct ttaatgatct
     4261 gctcacttat agacaccccc ctctatttat tctcgtttct tcttcttact attaatctgt
     4321 ctgttctcct cttgcctctt ctggtcttgt aacccttagc aggcataccc catggcgact
     4381 gaggaatatg gcctttacat ttaccttctc ctccgcccat cggatggtca accgggttca
     4441 tggtcatacc tcttacggtt ggtctgattc cgagccatct ccttcttccg gcctttccgt
     4501 gcacttcgtt cacatgatct tcatttccga tcatgcctac tgtggcataa catgtttccc
     4561 ttactcttct gagctcacct gaaggcattt tcaaaagagc gtaaccgctt tcttttgcca
     4621 taagctgtgc atatactccg gccgatctgg ctattttacc gcctctgttc ggttcaaact
     4681 caatattgtg aacaagcgta cccaacggaa tattggcaag cggaagagca tttccaggct
     4741 ttatttcggc cttggggctg ctgagcagtt cctgtcctac ctttacacct tttggagcaa
     4801 gtatgtatct cttttcgccg tcagtataga ctaaaagagc tattcttgcg cttctgttag
     4861 gatcgtactc tatcgaggct attttacacg gaatatcgta tttgtctctt ttgaaatcta
     4921 tgattctgta tcttctcttg tggccaccgc cctgatgtct tacagttatt ctgccgtggt
     4981 ggtttcgtcc tccattcgat tttaaaggag caagcagcga cttttccggt ttatctgttg
     5041 taatttctgc aaagtcgttg atgatcataa atcttcgtga aggagttgtc ggtttaaatt
     5101 ttttaagacc cattatttat cacctcttaa acgttgcctt gcaattcttt tattacatat
     5161 ccttgggcaa gcgtaactat agctttcttc catcctcttg tatatccttc aaatcttccc
     5221 attttcttag gttttgattt aacgttcatt atgttgactt tttccacttt cacattaaaa
     5281 agcttctcaa tggcagtttt aacttctgtt ttagttgcat cttttgccac ttcgaaagtg
     5341 tacttgcttt cacttatcag ctggtaactt ttttcggtga ggattggctt gagtacgata
     5401 tcgtaattga gtttattatt ttccattgtc cagcacctcc tcaatctttt ttactaattc
     5461 cttggtaagt acgatctttt cattgttgat aagatcaaaa acgttcaatc cgtcaatatt
     5521 catcttgccc tggttagggt tgtccgctat aatgaccttt accttaggaa tattattggc
     5581 agaaagcttt acgttatggt actcctcatt tttgaaagga agaacaaaaa gcactttctt
     5641 tccggtaagg cttatcttct caataacttc cttcatctgc ttggttcttg gggtatcaaa
     5701 ctttatatcg tcaatgacaa caatattgtt ttcccttagt cttacactca gtgctgactt
     5761 aagagcaagc ttcttcatct ttttgtttaa agacttatcg tagtctctcg gttttggtcc
     5821 gaaggtaaca ccgccatgtc tccatatagg tgatctggat gatcctgctc ttgctctacc
     5881 tttacgtttc tgcgcccaag gctttcgtcc
//
LOCUS       DAOSHR010000129         5851 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_12040_length_5851_cov_4.776743, whole genome shotgun sequence.
ACCESSION   DAOSHR010000129 DAOSHR010000000
VERSION     DAOSHR010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 5851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5851
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12040_length_5851_cov_4.776743"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(88..>1065)
                     /locus_tag="PLU28_07675"
     CDS             complement(88..>1065)
                     /locus_tag="PLU28_07675"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG34978.1"
                     /translation="QKYYDKARELEGIQKYEDAINYYIKAEKILTDHTPKNTYSDLYL
                     RRARLYYKIGDLNEAWYDYSTHINFFSQDYDAYNERGIVSYDSMDYETALGDYDAAID
                     ITQDKDYIFYNRALAYYALERYYEAIDDFDKVISFNSQDYEAISYKGLCYTALGEYEK
                     SISLQKEALEINPLYSIAYNEMGNASFYSFMDDDAIEYYTKAIGLEENPNYYFNRAKV
                     YLYNQRYDEAISDFIRTIELNPKDYEAFNQIGYCYYNMQEYIQAEEYFLSALDINYMY
                     TPACYNLGLVYENLKEYEKAVEYYNYVIDYAEPSDSLIEKAKERIKAIS"
     assembly_gap    1066..1165
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1300..3669
                     /gene="leuS"
                     /locus_tag="PLU28_07680"
     CDS             1300..3669
                     /gene="leuS"
                     /locus_tag="PLU28_07680"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HOG34979.1"
                     /translation="MYDFKSVQEKWQEYYNQVRPFEVDLKNAKKPFLNLMMFPYPSAA
                     GLHIGNMYSFMGSDIYGRYMKLKGYDVYEPIGFDAFGIHSENYALKVGEHPAVLTPKS
                     IEYFREKQLKKIGAIYDWKSEVNTSSPEYYKWTQWVFLQLYKAGLAEKRVSNVNWCPS
                     CKTVLADEQVIENKCERCSSEVEKREMSQWFFKITKYSEKLLDNLDKIDWTENTKNIQ
                     RSWIGKSKGASIKFRLDGSQKYIEVFTTRPDTIYGVTYIVVAPEYKNLEQLIKPEIKK
                     EFELFEESVRKMDMATRTSIKREKNGIFTGSYAVHPLTGKKIPVWIGDYVIADYGTGA
                     VMAVPAHDERDWVFAKKYDLPIKKVIECEDSQLPYTEYGVMINSDKYTQTKSIDFIEN
                     VGSVHPDFEAQTNFHLHDWCISRQRYWGPPIPVIYCDHCGTVPVREEDLPVMLPQTKD
                     YIPDGSGKSPLAKNEEFINTVCPKCGRPARRETDVSDNFLDSAWYFLRYCSPNDDKKA
                     FDTELVKKWYPVGMYIGGNEHANLHLMYSRFITMALHDLGYLPFEEPFSSFRGHGLIL
                     KDGAKMSKSKGNMINPNDYFETHGVDCLRTYLMFMGNFLEGGDFRDSGMDAIQRFLNR
                     VWDIASLPEGKCSDSVKTKMAQTINRFSYSIENMKYNAAVACLMEFSNEAVKESCMER
                     SVVVDLAKMLAIFAPFIAEEIHLMKGGKGTVLNAGFPSGYEKYLESASIDLPVQINGK
                     MRGIISVPKGLDSDALLCLLKENERFRSILEKPVKKVILVKDKIANIIM"
     gene            complement(3709..>4071)
                     /locus_tag="PLU28_07685"
     CDS             complement(3709..>4071)
                     /locus_tag="PLU28_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34980.1"
                     /translation="GLVDIAVAKDNVYAPTNKVNISDNVSGLKLVEFNQLTKLPEGGY
                     SFTKDIADAIPASGYTVSGAGVTSLKGNSRLNGNPTGTIVAPEFLIAGDINVPFDMGP
                     ADNYVVVFDIVVSPKGKF"
     assembly_gap    4072..4171
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4340..4852)
                     /locus_tag="PLU28_07690"
     CDS             complement(4340..4852)
                     /locus_tag="PLU28_07690"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HOG34981.1"
                     /translation="MILKYKDKEPKIGKNVFIAPGAIIIGDVEIKDGASIWFNCVVRG
                     DMAPVEIGENSNIQDNCTVHTAENAPVKIGNNVTVGHNAIVHACSIEDDVLIGMGAII
                     LDNAVIKSGTVIAAGSIVKEKNVMGPDELYAGIPASKKKEFDASHRKELKDHATEYSR
                     LAWEYIQESK"
     gene            complement(4836..>5851)
                     /locus_tag="PLU28_07695"
     CDS             complement(4836..>5851)
                     /locus_tag="PLU28_07695"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOG34982.1"
                     /translation="SNPPSIYKFNGIYFIFVKFIVFENSILTIGGPSLEYTAFIETFD
                     YVTVMMIIFGSFFGLFISYVLAVRTLRPVIHISRQISKIDVESLEQRIPQQETLEFQI
                     LSDRLNSMLQRLQNGYETQKQFVSDVSHELRTPLTTVSGYISMLKRWGLKDEAILNES
                     IQSLEKSTEYLKDLVEKLLLLSKPDYNMEMEVIDLSLVVKDVTDIYDKNSVNINTYGQ
                     PFEVLTSEKYLSIVLKVLIDNALKFSEKKQVDISFGDNRLSIRDYGIGIEKEKLDKIF
                     DRFYKGSFSRNEGGHGLGLSIAKKISEKLGIDIEVESEVNSGSTFTLIFKEKKEESDD
                     TQV"
BASE COUNT         1707 a         1042 c         1096 g         1806 t
ORIGIN      
        1 tccaaaaata acgtatatct atatcttaaa taaagaatct cctcactttt agggaggaga
       61 ttttttctat acatgactgc aaaaaattta gcttatagcc ttgattcttt cttttgcttt
      121 ttcaatcaga ctgtccgaag gctcggcgta atcaattacg tagttatagt attcaacagc
      181 tttttcatat tctttaagat tttcatatac aagacctagg ttatagcatg ccggagtata
      241 catatagttt atatccaatg cggacaggaa gtattcttcg gcctgaatgt actcttgcat
      301 attataatag cagtatccta tctggttaaa tgcttcataa tcttttggat ttagttctat
      361 ggttcttatg aaatcagaaa ttgcttcatc gtatctttga ttgtaaaggt atacttttgc
      421 cctgttaaag tagtagtttg gattttcttc aagtccgata gccttggtgt aatactctat
      481 tgcatcatca tccataaaac tgtagaaact ggcatttccc atttcattgt aagcaatcga
      541 ataaagggga tttatttcaa gagcttcttt ctgtaatgaa attgattttt catactctcc
      601 cagagccgtg tagcatagac ctttatagct tatagcttca taatcctgag aattaaagga
      661 aattacttta tcgaaatcgt ctattgcctc atagtatctt tcaagagcat aataggccaa
      721 agccctgtta tagaatatgt agtccttgtc ctgagtaatg tctattgcag catcgtagtc
      781 acctaaagca gtttcgtaat ccattgaatc gtagcttact ataccgcgtt cattgtaagc
      841 atcatagtcc tgactgaaaa agttgatatg agtgctgtaa tcataccatg cttcattaag
      901 gtctccgatt ttgtagtaaa gtcttgcccg tctgagataa agatctgagt aagtattttt
      961 cggagtatga tcggtaagta ttttttctgc tttaatataa taattgattg catcttcgta
     1021 cttttggata ccttcaagtt ctcttgcttt atcataatac ttctgnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnttacc tcacctccat atctttttat attattataa
     1201 catagcttta aggcttttat atccatatat ttgattttat caacaatgct ctggtataat
     1261 taaataatat caaattctgt ttttgtcggg aggcatgtta tgtacgattt taagtcagtt
     1321 caggaaaagt ggcaggagta ctataaccag gtcaggccat ttgaggtgga tctgaaaaat
     1381 gcgaaaaaac catttttaaa tcttatgatg tttccatacc cttccgctgc cggactccat
     1441 attggaaaca tgtactcttt catgggttcg gatatctatg gcagatacat gaagcttaag
     1501 ggatacgatg tttacgagcc gattggtttt gatgcttttg gaatccacag tgaaaactat
     1561 gcactcaagg taggagagca tcctgccgta ctaacgccca aaagtataga atatttcaga
     1621 gagaagcagc taaaaaaaat aggtgcaatc tatgactgga aaagcgaagt aaatacatct
     1681 tctcctgaat actataaatg gactcaatgg gtttttcttc agctttataa agcaggattg
     1741 gcagaaaaga gagtatcgaa tgtcaattgg tgtccatcat gcaagacagt gcttgcagat
     1801 gagcaggtaa ttgagaataa gtgtgaaaga tgctcaagtg aagtcgaaaa gcgtgaaatg
     1861 agccagtggt tctttaaaat aactaaatat tccgaaaaac ttttggataa tcttgataaa
     1921 atagattgga cagaaaatac caaaaacatt cagagaagct ggataggcaa gtctaaaggt
     1981 gcaagtataa agttcagact tgacggcagc caaaaatata ttgaggtctt tactacaagg
     2041 ccggatacta tatatggtgt tacctacatt gtggtcgccc ccgagtataa gaaccttgag
     2101 cagcttataa agcccgaaat caagaaagag tttgaacttt ttgaagagtc tgtaagaaag
     2161 atggatatgg ctacaagaac ttccataaaa agagaaaaga acggtatttt tacaggcagc
     2221 tatgcggtcc atccccttac aggcaagaaa ataccggtct ggataggaga ttatgtcata
     2281 gccgattacg gaactggtgc cgttatggca gttcctgccc atgatgaaag agattgggtt
     2341 tttgcaaaaa aatatgatct tcctataaaa aaagtaattg aatgtgagga ctcacagctt
     2401 ccatatacag agtatggagt tatgataaac agtgataagt atacgcaaac taaaagtatt
     2461 gattttatag aaaatgtagg ttcagttcat ccggattttg aagcacagac caactttcat
     2521 ctccacgact ggtgtatttc caggcagagg tactggggac cgcccattcc tgttatttac
     2581 tgtgatcact gcggtacagt tccggtaaga gaagaggatc tgcctgtcat gctgccccag
     2641 accaaagact atattcctga cggaagcgga aaatctccac tcgctaaaaa tgaagagttt
     2701 attaacacag tgtgccccaa atgcggaaga cctgccagaa gggagacaga tgtaagtgac
     2761 aactttcttg attcagcttg gtatttttta aggtattgtt ctcctaatga cgataagaaa
     2821 gcttttgata ctgaactggt gaaaaaatgg tatccggtgg ggatgtatat aggcggtaat
     2881 gaacatgcca acctccatct gatgtactca agatttataa ccatggcgct tcatgacctg
     2941 ggatacctgc catttgagga acctttttcc tctttcaggg gacacggact tatactcaaa
     3001 gacggtgcaa agatgagcaa atctaagggc aatatgataa atcccaacga ttactttgag
     3061 actcacggag tggactgtct cagaacttat ctaatgttta tggggaattt tcttgaaggc
     3121 ggagatttca gagattcagg catggatgcc attcaaagat ttcttaacag ggtatgggat
     3181 atagccagtc ttccagaggg gaaatgcagt gactctgtaa aaaccaagat ggctcaaact
     3241 ataaacagat tcagctattc aatagaaaat atgaagtata atgccgcagt tgcctgcctt
     3301 atggagttca gcaatgaagc agtaaaagaa agctgcatgg aaagatctgt ggttgttgac
     3361 ctggccaaga tgcttgccat atttgcaccg tttatcgctg aagagataca tcttatgaaa
     3421 ggcggtaaag gtacagttct taatgccggt tttcctagtg gatacgagaa gtacttggaa
     3481 tctgctagta tagatcttcc tgttcagata aacggaaaaa tgcgcgggat tataagtgtt
     3541 cccaaaggat tggacagtga tgcactctta tgcctgctta aagaaaatga aagattccgc
     3601 tcaatccttg aaaagcctgt caaaaaagtc attctggtta aagataagat agcaaatata
     3661 ataatgtaaa aaaactccct gagatcttct cagggagttt tgattatttt agaactttcc
     3721 ttttgggctt actactatat caaatacaac tacgtaattg tctgcaggtc ccatatcgaa
     3781 aggaacgtta atatctccgg ctataaggaa ttcaggggct actattgttc cggttggatt
     3841 tccgttaagt cttgagtttc ccttgaggct cgtcacacca gctccgctta ctgtatatcc
     3901 ggaagcagga attgcatcgg ctatatcttt tgtaaaactg tatccgcctt cgggaagctt
     3961 ggttagctga ttgaattcaa ctaattttaa accgcttaca ttatcgctta tattaacttt
     4021 atttgtagga gcataaacat tgtcttttgc aactgctatg tctacaagac cnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naataactgc cataagtttt ttcataatcc
     4201 gacacctccg ggtatcaagt tttattgttt tctgataaaa gaataccctg aatgtgttaa
     4261 cggtcaagca gatatattac gaaataaaaa aattataatt aagataaagc aacataaaac
     4321 aaagttagtt accaaaaagt tactttgatt cttggatata ctcccaagcc aaacgtgagt
     4381 actcggttgc atgatctttt agctcttttc tgtgggatgc atcaaactcc tttttctttg
     4441 aagccggtat gccggcataa agttcatccg gtcccataac gtttttttcc ttgacaatac
     4501 ttcccgcagc aataaccgtt ccggacttta tgactgcgtt atccaaaatt atggctccca
     4561 tccctattag tacatcatct tctatgctgc aggcatgtac gatggcatta tgtcctacag
     4621 taacattatt tcctatcttc acgggtgcgt tttcggcggt atgaaccgta cagttatcct
     4681 gaatatttga attttctcct atttcaaccg gagccatatc tccgcgtaca acacagttga
     4741 accatatgct tgctccgtcc ttgatctcaa catctcctat tataattgct cccggagcta
     4801 tgaaaacatt ctttcctatt tttggttctt tatccttata cttgagtatc atctgattcc
     4861 tccttttttt ctttgaagat aagtgtaaag gtgcttccgg aatttacctc tgattctact
     4921 tctatatcta tgccgagttt ttcggaaatt tttttggcga tggaaagtcc aagaccatgt
     4981 ccgccttcat ttcttgaaaa gcttccttta tagaatctat caaatatctt atcaagtttt
     5041 tctttttcta tacctatacc ataatccctt atactcaaac ggttatctcc gaatgagatg
     5101 tctacctgct ttttttcaga gaacttcagt gcattgtcaa taagaacttt aagaactatg
     5161 gaaagatact tctccgaggt cagtacttca aagggctgtc cgtatgtgtt tatatttacg
     5221 gaatttttat cgtatatatc agtaacatct tttactacca acgaaagatc tataacttcc
     5281 atttccatgt tatagtccgg tttacttagc agaagaagct tttcaacaag atccttgagg
     5341 tactctgttg acttttcaag actttggatg gattcgttta gtatggcttc gtctttaagt
     5401 ccccaacgct ttaacatgga tatatacccg gataccgttg taagaggagt acgtaattca
     5461 tgagaaacat ccgatacaaa ttgtttttgt gtttcatatc cgttttgcag tctttgaagc
     5521 atggagttga gcctgtctga cagaatctga aattctagtg tttcttgttg cgggatccgt
     5581 tgttcaagag attctacatc tatttttgaa atctgtctgg atatgtgtat tacaggtctt
     5641 aaagttctga ctgcaagtac ataggagata aaaagcccaa aaaaagatcc gaagattatc
     5701 atcatgacgg taacgtagtc aaaggtctct ataaaggctg tatactcaag agaaggtccg
     5761 cctatggtca gaatggagtt ctcaaaaaca atgaacttga caaaaataaa gtatattccg
     5821 ttaaacttgt atatggatgg tggatttgag a
//
LOCUS       DAOSHR010000130         5844 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_12070_length_5844_cov_3.509933, whole genome shotgun sequence.
ACCESSION   DAOSHR010000130 DAOSHR010000000
VERSION     DAOSHR010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 5844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5844
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12070_length_5844_cov_3.509933"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1892
                     /locus_tag="PLU28_07700"
     CDS             <1..1892
                     /locus_tag="PLU28_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34983.1"
                     /translation="VASTKVTAGKYVGGFVGLNEKLIDQCSVRYLQNATALNNSDAAG
                     GFAGRCDTFNGSIQNSYVKGSMTWNINASVGPSGFISSGNGATYYCYASVTGADGPRP
                     GTDTSIYGFSGQFFNTVDSYWDGRCQNTRAGNGGQVIESDKIALKTSYKNWDFGQVWF
                     MFEDLPFFERNIVKFEAGDGSAANPYEITTADQLMAMRFRLDKHYILKNDIDLSTAYW
                     IPIGKNKDGFIGVFDASNKTLRGLTIENSEVSEIGLFGCIGKGTVKNLVIENFNIAVK
                     SNAGLLAGTIEGETVADKNDFTKYAVIENIRAGGIINATDYTGGIVGKASNATMNNIE
                     STVQLTAASGQYNGGIAGKMERVNMQDVSYNGTLNGSEYVGGIAGYYYSLIPVNEPNN
                     ISKDLSSQGSIKGQKYVGGMFGTVYGGLFENLLSLAGVESKFDNTKDVNYISTGGIAG
                     MTGDATFTNLSCTGEVKGYKNVGGLFGIIQSGTVLESEFSGGVSGKDYTKNVGGVCGW
                     GNLALIERCASYNFGLAGKIESSTIKNSYSSTNKSYGGALIAYDAESTRIENCYSEQT
                     FISYRYNGCTGTSNYYTYSDVSAELAHKKTEAEMKNQATYVGWDFTNIWKIDPAQNNG
                     YPTLR"
     gene            2037..2882
                     /locus_tag="PLU28_07705"
     CDS             2037..2882
                     /locus_tag="PLU28_07705"
                     /inference="COORDINATES: protein motif:HMM:NF004862.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HOG34984.1"
                     /translation="MYKILYNNEVAKGIYEMRILAPLVARSAKPGQFVILIIDEKGER
                     IPLTVVDTDPIEGWVCVVVQSVGHSTNKLTALKESDYIRDFAGPLGKESEFVHKTLEE
                     LKDKKIVFVGGGVGAAPVYPQVKYLSERGIRTEVILGFKSAEYIILEKEFRMLNAAVH
                     ICTDDGSIGFRGLVTQKLKLLLEKEHIDEVVTIGPMIMMKNVSLLTKEYGVPTVASLN
                     TLMVDGTGMCGGCRVTVGGKTLFTCVDGPEFDAHLIDFDQAMSRQNIYKDEEKHSCRL
                     DGEIK"
     gene            2879..4264
                     /gene="gltA"
                     /locus_tag="PLU28_07710"
     CDS             2879..4264
                     /gene="gltA"
                     /locus_tag="PLU28_07710"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HOG34985.1"
                     /translation="MNKSRVPVNEQDPLTRKENFSEVCYGYSLEQAFEEACRCLNCKN
                     PLCVSKCPVGIDIPGFISHLKSRDLAGAYEVISRYTSLPAVCGRVCPQEEQCESGCVL
                     GKKSEPVAIGKLERYVGDWALEHDVKSMQTKEKNGHKVAVVGSGPAGLTCARELAAMG
                     YDVTIFEALHEPGGVLAYGIPEFRLPKDKVVRKEIRRIQEMGVRIETDVIIGRTLTID
                     ELLTHEKFEAVFIGSGAGLPRFMNIKGENSKGVFSANEILTRINLMKAYNKEYDTPVH
                     LGKKVVVVGGGNVAMDAARASLRMGCEVTVVYRRSEEELPARLEEVHHAKEEGIVFRL
                     LTNPVEIINGENGWVAAVRCIKMELGEPDSSGRRRPVQVKSSEFEIPADTVIMAIGTS
                     PNPLITSVTPDIQINLERCIVTDERCRTSKAAVYAGGDAASGAATVILAMQAGKKAAA
                     SIDEMIKSNNQ"
     gene            4298..5614
                     /gene="glmM"
                     /locus_tag="PLU28_07715"
     CDS             4298..5614
                     /gene="glmM"
                     /locus_tag="PLU28_07715"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOG34986.1"
                     /translation="MKRLFGTDGIRGIVNDELTVDLAMKLGNSVARLYNGKYKTLLIA
                     MDTRSSGQMFEAAITTGAISAGMNVIQCGVIPTPCLAFLTKQKNAVGVMISASHNPSI
                     YNGLKVMAQGYKIPDEEEVEIENLMLKDHMEYSPYGEIGTVEKDSVKELYFDYAISTF
                     KGIKSSFRFAVDTGNGAAGAIAGRIFSELGLDCDIYFNSPDGFNINDRCGSTAPETIS
                     KIVKEKGYDFGVLFDGDADRCLFVDRNGILVDGDKLMAVNAVKMKQQKRLTGETVVST
                     VMSNLGFEEYLHGHGVKLYRTQVGDKYVLEKMLEISSNIGGEQSGHIIMLDRSTTGDG
                     LITALETIDTMNFFSKTIDRLVGEIPEYPQVLKNVAVKDKKKIMTDERMTSALKKYET
                     NKEIRLLVRPSGTEHKIRVMTEGKNEKLVNEVAQEFAELIDIIDKE"
BASE COUNT         1943 a          919 c         1446 g         1536 t
ORIGIN      
        1 atgttgccag tacaaaggta accgccggaa agtatgtagg agggtttgta ggacttaatg
       61 aaaagcttat agatcagtgt agtgtaaggt atctccaaaa cgctactgct cttaataata
      121 gtgatgcggc cggtggattt gcaggtaggt gtgacacttt taacggctca atacaaaaca
      181 gttatgtaaa aggaagtatg acatggaaca ttaacgcatc agtaggaccc agtggtttta
      241 taagctcagg aaacggagca acgtactatt gttatgcaag cgttaccgga gctgatggtc
      301 caagacccgg aactgatact tcaatatatg gattttcggg acagttcttt aatactgttg
      361 attcatactg ggacggtcga tgtcagaata cacgtgccgg taatggcggt caggtaatcg
      421 aatcggataa aatagcttta aagacatcat ataaaaactg ggattttggt caggtatggt
      481 ttatgtttga agatcttccg ttttttgaaa gaaatatagt aaagtttgaa gctggagacg
      541 gttcagcagc aaatccgtat gaaataacga cagcagatca gctcatggct atgaggttca
      601 gactggataa gcattatatc ctcaagaacg atatagatct ttcaactgca tactggatac
      661 ctattggcaa gaataaagat gggttcatag gagtctttga cgcaagcaat aaaacattaa
      721 gagggttgac cattgaaaat tccgaagtgt ctgaaatagg attattcggt tgtataggaa
      781 aaggtactgt aaagaatctt gttatagaaa atttcaatat agcagtcaaa tcaaatgccg
      841 gacttcttgc cggaacaata gaaggagaaa ccgttgcgga taagaatgat ttcaccaagt
      901 acgcggtcat tgaaaatatc cgtgcaggag gcatcatcaa cgcaacagat tatacaggtg
      961 gcatcgtcgg taaagcgagc aacgcaacta tgaacaatat tgaatcaacg gttcagctta
     1021 ccgcagccag cggacagtat aacggcggaa ttgcaggaaa aatggaaaga gtcaatatgc
     1081 aagatgtatc gtataacggt accttgaatg gttcggagta tgtgggtgga attgccggat
     1141 attattactc gcttattcct gttaatgaac ctaacaacat atcaaaagac ttatcttcac
     1201 agggcagcat aaaaggacaa aagtatgttg gagggatgtt cggaacagta tatggaggtc
     1261 tttttgaaaa tcttttgtct cttgccggtg tggagtcaaa gttcgataat acaaaagatg
     1321 tgaactatat cagtactggt ggaatagcgg gtatgacagg agatgctact tttacaaatc
     1381 tttcctgtac tggagaagtt aaaggatata aaaatgtagg cggtctgttt gggattattc
     1441 aatcaggtac tgttctagaa tcagagtttt caggaggagt cagcggtaag gattatacaa
     1501 aaaatgtcgg tggagtatgt ggctggggaa atttagcttt gatagagaga tgcgcatctt
     1561 ataactttgg acttgccgga aagattgaat caagtacaat taaaaacagt tattcctcta
     1621 ctaataaatc atatggaggt gctttgattg cgtatgatgc agaaagtaca agaattgaaa
     1681 actgctactc tgaacaaact ttcataagct acagatataa tggctgtaca ggaacttcaa
     1741 attattacac ttactctgat gtttctgcag agctggcaca taagaaaaca gaagctgaga
     1801 tgaaaaatca ggcaacctat gtaggctggg actttacaaa catatggaag attgatcctg
     1861 cccaaaacaa tggttatcct accttaaggt aaagattcaa aaattaagcc tcccgaaagg
     1921 gaggcttttt attgacaata tatatattaa taaaaaaata aaatttattt agtttcaaaa
     1981 aacaaaatat tatgatatat ttttatatgg ataaaaagtt tgaaaaggag gaagtaatgt
     2041 ataaaatttt atataataat gaagtagcca aaggtatata tgagatgcgc atccttgctc
     2101 cgttggttgc cagatcggcc aagcccggac agtttgtgat tctcataatt gatgaaaaag
     2161 gcgaaagaat accgcttact gttgttgata ccgaccccat tgaaggctgg gtatgtgttg
     2221 ttgttcagtc agtaggtcat tctacaaaca agttgacagc tttgaaagaa tctgattata
     2281 taagggattt tgcgggaccg cttggtaagg agtcggagtt tgttcacaaa actttggaag
     2341 aactgaaaga taaaaagata gtatttgttg gaggaggagt tggtgctgct ccggtatatc
     2401 cgcaggtaaa gtatctgtcg gaaagaggta taagaactga agttatactg ggttttaaaa
     2461 gtgcagagta cataattctg gaaaaagagt tcagaatgct gaatgcagct gttcatatat
     2521 gtacagatga cgggagcata gggttcagag gcctggtcac tcaaaagctt aaactgttgc
     2581 ttgaaaaaga gcacatagat gaggtagtga ctataggtcc tatgattatg atgaaaaatg
     2641 tttcacttct gactaaagaa tacggtgttc caactgtggc cagtcttaat actcttatgg
     2701 ttgacggaac aggaatgtgc ggaggatgca gagtgactgt aggagggaaa acattgttta
     2761 cttgtgttga tggtccggag tttgatgcgc atctgataga ttttgaccag gcgatgtcca
     2821 ggcagaatat atataaagat gaggaaaagc acagctgcag gctggacggt gagataaaat
     2881 gaataagtcc agagttccag taaatgagca ggatccattg acacgaaagg aaaatttttc
     2941 tgaggtatgt tacggttatt cattagagca ggcctttgaa gaagcgtgca ggtgtctaaa
     3001 ctgtaaaaat ccgttatgtg taagtaaatg tcccgtagga atagatatcc ccggatttat
     3061 cagtcacctt aagagtagag atcttgccgg tgcatatgag gttatctcaa gatatacttc
     3121 tctgcctgcg gtctgcggca gggtttgtcc tcaggaggaa cagtgcgaaa gtggatgtgt
     3181 tcttgggaaa aaatctgaac cagttgccat aggcaagctt gaaaggtacg ttggggactg
     3241 ggcgcttgaa catgatgtaa aatccatgca gacaaaggaa aagaacggtc ataaggttgc
     3301 agttgtagga agcggtcctg caggacttac gtgtgcacgg gaacttgcag ccatgggata
     3361 tgatgtaact atatttgaag cacttcacga accgggagga gtgctggctt acggtatacc
     3421 cgagttcagg cttcccaaag ataaagttgt gaggaaagag attagacgta tacaagagat
     3481 gggagtaaga atagagacag acgttataat aggaagaaca cttacaatag acgaacttct
     3541 aacgcatgaa aagttcgaag ctgtttttat aggctccggg gcaggtcttc caaggtttat
     3601 gaatataaag ggtgaaaaca gcaagggagt tttttctgcc aacgagatac ttaccagaat
     3661 taatcttatg aaggcgtata ataaagagta cgatacacct gtacatcttg gaaaaaaggt
     3721 tgtagtggta ggaggcggca acgttgcgat ggatgctgca cgtgcaagct taagaatggg
     3781 ctgtgaggta acagttgttt acaggagaag cgaagaagag ctgcctgcaa gacttgagga
     3841 agttcatcat gcaaaggaag aaggcatagt attcagactg cttacaaatc ctgtagagat
     3901 tataaacggt gaaaacggct gggttgcagc agtgagatgt ataaagatgg aactcggtga
     3961 gccagacagt tccggtagga ggagacctgt acaggtaaag agctctgaat ttgaaatacc
     4021 ggccgatacg gtaataatgg caataggtac ctctcccaat cccctgataa cctctgttac
     4081 accagacata caaattaatt tggaaagatg tattgtaacc gatgaaaggt gcagaacctc
     4141 caaggctgcc gtttatgccg gaggagatgc cgcaagcggt gctgcaacgg tgattcttgc
     4201 catgcaggct ggaaaaaaag ctgcggcgtc tatagacgaa atgataaaaa gtaataacca
     4261 atagttattt aagattatac ttaggaggga agttatcttg aaaagattat tcgggactga
     4321 cgggataagg ggaatagtaa atgatgaact gacagtcgat cttgccatga agcttggcaa
     4381 ttcagtagca cggttatata acggtaagta taaaacactt cttatagcca tggatacaag
     4441 aagttcaggg cagatgttcg aggcggcaat aaccacaggg gctatatctg ccgggatgaa
     4501 tgtaatacag tgcggggtta ttccaacccc ttgccttgcc tttctaacaa agcagaaaaa
     4561 tgccgtagga gttatgatat ctgcttctca caatccgtcc atatacaacg gtcttaaggt
     4621 aatggctcag ggatacaaaa tacccgatga agaagaggta gaaatagaaa atcttatgct
     4681 taaggatcat atggagtaca gtccatatgg tgagatagga actgttgaga aggactctgt
     4741 aaaagaactt tactttgatt atgcgataag tacctttaaa gggataaaga gcagcttcag
     4801 atttgccgtt gatacaggca acggtgcagc cggtgcaatt gcaggcagaa tattcagtga
     4861 actgggactg gactgtgata tatactttaa ttcgcccgac gggttcaata taaatgaccg
     4921 ctgtggttca accgctccgg aaactatttc aaaaattgtt aaggaaaaag gatacgattt
     4981 cggcgtactt tttgacggtg atgctgacag atgtcttttc gtggatagga acggtatctt
     5041 agttgacgga gataagctta tggctgtaaa tgctgtcaaa atgaagcagc agaaaagact
     5101 taccggagag acagttgtat ccactgtaat gagtaatctt ggattcgaag agtatctgca
     5161 cggacatgga gtaaagcttt accgtacaca ggtgggagac aagtatgttc ttgaaaagat
     5221 gctggagata tcttcaaata tcggtggcga acagtccggg catataataa tgcttgacag
     5281 gtcaacaacc ggtgacggtc tgataacagc gcttgaaacc attgatacta tgaatttttt
     5341 ctctaaaacc atagaccggc ttgtgggaga gattccagag tatcctcagg ttctgaagaa
     5401 tgttgcagtt aaagataaaa aaaagattat gacggatgaa agaatgacct ctgcattgaa
     5461 aaaatatgag acaaacaaag agatccgtct tttggtaaga ccatcaggta cagaacataa
     5521 gataagggtt atgacggaag gaaagaatga aaagctggta aatgaagtag cgcaggagtt
     5581 tgcagaactt atagatatca tagataaaga atgaattgaa tccctagcgg gattcaattt
     5641 tttttacata gaaggtttaa acttaacgga aagttgaaaa aaaggtcaaa cagcacaaaa
     5701 gataagttta tctgcaatct tttcaaattt actccaaaat acagcgatga gataaggttt
     5761 tttgaagtgc tataatggta atatcacaca taaggcggtg taaaaatgtt agcaaaggat
     5821 tatctgaaaa gagaggtaaa agaa
//
LOCUS       DAOSHR010000131         5692 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_12518_length_5692_cov_6.360653, whole genome shotgun sequence.
ACCESSION   DAOSHR010000131 DAOSHR010000000
VERSION     DAOSHR010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 5692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5692
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12518_length_5692_cov_6.360653"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="PLU28_07720"
     CDS             <1..397
                     /locus_tag="PLU28_07720"
                     /inference="COORDINATES: protein motif:HMM:NF039996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6115 domain-containing protein"
                     /protein_id="HOG34987.1"
                     /translation="DKFVDENKKTLKTLESLNDEETLQKLKKLDKPKKTTSKKTAAAS
                     KKTSAPRKKPTALPTEQTVLESTTQEKESITPQAEISDIAPSDSVSGQSKKEKIFSLY
                     SRGIAPSDIAKELGLGTGEVNLYIDLYKK"
     gene            complement(412..1203)
                     /locus_tag="PLU28_07725"
     CDS             complement(412..1203)
                     /locus_tag="PLU28_07725"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK/PrmC family methyltransferase"
                     /protein_id="HOG34988.1"
                     /translation="MTLREIYKQIPGDLKVSRFSLAKAVSYIYNEDPSRYIFNDSIDI
                     PLSIAQKIVKHFVSGYPLAYIANRVSFCSSEFYVDERVLIPRQETQDLAEYAMKMIDS
                     CKIKSVADIGTGSGVIAVILKKFNPDLHVFASDISSQALEVAKYNACKNMVKIDFREG
                     MFMEPFVGEKVDMIVSNPPYVHSSYNNPDLQYEPANALYAGEKGQDFFIGLSKYEEVK
                     KVKYMLFETTEFFIEETCKILSQFGDIEKLTDRFGIYRFIKVLRN"
     gene            complement(1205..1510)
                     /locus_tag="PLU28_07730"
     CDS             complement(1205..1510)
                     /locus_tag="PLU28_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HOG34989.1"
                     /translation="MGKITQEDVFKALSNVYDMEIGFDIVSLGMVYEVKVDDEDNVKV
                     IMTLTTPMCPLAGFITEDARSKVKAVSGVNDVKVELSFDPPWSPDMAKEEVRRVLGI"
     gene            complement(1526..2524)
                     /locus_tag="PLU28_07735"
     CDS             complement(1526..2524)
                     /locus_tag="PLU28_07735"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOG34990.1"
                     /translation="MKKLFFLVFVTVLSVGLFAVVTYGDLGQNIAFKANGETVDNDLL
                     TARSQSLVILQSLKLNFPDFYDTLTKTATGIELLNEYSEYVRDKLINEVLFIQFVESK
                     GIDLQRTTTKDYFKQNIEQIFKDSSFTQEDIDMYLFTKGYLNIASYINDKFYSALYKN
                     AILKLHEKITLSYSFTDSEMKKEYDTNKKSYVSRPYASIKLLYFDKNDEAKQYYQKII
                     DGVYTFDEIYALLLKTSYAQETTVYLDEESELSKVLKARAPGSIVEPSAIQEKTVIIR
                     IEKKAVSKQLTYEEAKDTILINLRDIKAKDYFDKILPQEFEVFKNNASVVINSKNF"
     gene            complement(2521..3255)
                     /locus_tag="PLU28_07740"
     CDS             complement(2521..3255)
                     /locus_tag="PLU28_07740"
                     /inference="COORDINATES: protein motif:HMM:NF015758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HOG34991.1"
                     /translation="MDEKRKEVYSSEFIRLLEIMEQLRSEKGCEWDKKQTHQSLRPYA
                     IEEAYEVVESIDSGDTSELKKELGDLMFQCIFHAQIAYEDKEFDIGDVLCSVSDKMVR
                     RHPHVFSDGKGYSYEQWEMLKSKEDNEKMCRIGKYKRGAPPIIQLRRLFENSLAVGFD
                     PYFGYDVKKLISEDLYSGNYKGVLEKIIYMSVKESINLESLVNQASKEFHERFVSLEK
                     LAGEKFNEYSEEDKIKMWDKIRGEKG"
     gene            complement(3269..5482)
                     /locus_tag="PLU28_07745"
     CDS             complement(3269..5482)
                     /locus_tag="PLU28_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34992.1"
                     /translation="MKRVFFCVGIIICACSVFSIILWDGQAGKQDHFIRSIPRFYDKN
                     SYVFGSSFSEVQESGSLKIIDRTDGHTRYSFQTESPVFPCFCKDIDNDGESEIVAVLA
                     QGPVQVFKVIGGTLKMTTSFSPVFTDLPFNVTIQDGSIVCADDYMDVFSAGDRILKVQ
                     GRDVLSWWDIMQILKDMKKGEYVTVNVLDKNSKIKRSSVELTENEDYFMAPSSFFCDS
                     AEWQSDRYVISASMNGFIYLWDYLGNIVSRVYIGYEYYSVPPQPVDINYDGKYELLMT
                     SFEGVSTLLDASGEILWQFELGDDIFSLPLICDINSDGDFDIVYCTLSGKVIYSDKFG
                     NARLISELKSRVLSNPAASDLYLSGIINIITADESGKMSIFDPDGNLVKQKEIPSEAF
                     FSSPLVIGLYDNETAWSNKIFLVSSSGKVYRYDTLTDELKFKADIIDTCSANVVIDDV
                     DIDGVAELLIPCESGDVKAMQIGEYSRLTPWTEPNGDHFGSYVSYINTPLKVAQEFET
                     FEASDIRSCSVAISSFFRFSDNEEYVFSASGSIFYEDGESVYILTSLSACGIIPVMTK
                     LNEQNFSYMDVRLKVGDQRVAPDSIYINRKLKDLCIMKVSKDRIKQDISPASLAFSQS
                     NAYELYDFIGQRSIKLGLSESKTGYVNFFTYEYRDIISEEALLGGSGIFDDAAHICAF
                     IGYSPQGQMIAITADEVLESFQAQEYIKLVYSNEKNNDAAGGLLKKIAGYYGMYD"
     gene            complement(5495..>5692)
                     /locus_tag="PLU28_07750"
     CDS             complement(5495..>5692)
                     /locus_tag="PLU28_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOG34993.1"
                     /translation="QKYSQYGFEKHKGYPTKLHEQMVKKYGIFNEFRITFDPVRTLIV
                     NGQIIADRSEFSAQRLLRIGM"
BASE COUNT         1774 a         1240 c          870 g         1808 t
ORIGIN      
        1 cgataagttt gttgatgaaa ataaaaaaac tctgaaaact cttgaatcct taaatgatga
       61 agaaaccctt cagaaattaa aaaaattgga taaacctaaa aaaacaactt caaagaaaac
      121 agccgctgca tccaagaaga cctcagcacc aagaaaaaag ccaacagcgt tgcctactga
      181 acaaacagtc ttagaatcaa caactcaaga aaaagaaagt atcaccccac aggctgaaat
      241 atccgatatt gctccttcag atagtgtttc gggacagagc aagaaagaaa aaatattctc
      301 cctttattcc agaggcatag ctccttcaga tatagcaaag gagctgggat taggaaccgg
      361 agaagttaat ttgtatattg atctttataa aaaataatca gaaataactt ttcagttacg
      421 gagaactttt ataaacctgt aaatgccaaa tctgtcggtg agcttttcga tgtcaccaaa
      481 ttgggagaga atcttgcagg tttcttctat aaaaaattct gtcgtctcaa aaagcatgta
      541 ctttactttc ttgacttctt cgtactttga aagtcctata aaaaagtcct gacctttctc
      601 acccgcataa agcgcatttg cgggctcata ctgaagatcg ggattattat agcttgaatg
      661 tacatatgga ggattagaaa caatcatatc aactttctct cccacaaaag gctccataaa
      721 cataccttct ctaaaatcaa tttttaccat atttttgcag gcgttgtact ttgcaacctc
      781 aagggcctga gaagaaatat cggatgcaaa aacatgaaga tcgggattaa acttttttag
      841 aataacagct atgactcctg aaccagtacc tatatcggca actgatttta ttttacagct
      901 gtctatcatt ttcatggcat actcggcaag atcctgagtc tcctgtcttg gaataagaac
      961 gcgttcgtca acataaaatt ctgaagagca aaaactgacc ctattggcta tatatgcaag
     1021 cggatatccc gatacaaaat gctttactat tttttgagct atagataggg gaatgtctat
     1081 gctatcgtta aatatatatc tagaaggatc ttcattgtag atataagaaa cagcttttgc
     1141 aagagaaaaa cgggaaactt ttaagtctcc cggtatttgt ttgtatattt ctcttaaagt
     1201 cattttatat tccaagcacc cttcttactt cttcttttgc catatcagga ctccacggcg
     1261 gatcaaagct cagttccacc ttgacgtcat ttactccgga aacagccttg accttgcttc
     1321 ttgcatcttc ggttataaat cccgcaagtg gacacatggg tgttgtaagt gtcattataa
     1381 ctttcacatt atcttcatcg tcaactttaa cttcatatac cattcccaac gatactatat
     1441 caaaaccaat ctccatgtca tatacattag aaagagcctt aaaaacatct tcctgagtta
     1501 tttttcccat attattcttc ctccatcaga aattttttga attaattaca acagaagcat
     1561 tattcttaaa aacttcaaac tcctgtggca gaattttatc aaaataatct ttagccttta
     1621 tatctctgag gttaattaaa atagtatctt ttgcctcttc gtaagtaagc tgtttggata
     1681 cggccttctt ttctatcctg attataacgg ttttttcctg aatggcagaa ggttcaacaa
     1741 tacttccggg tgcccttgct ttaagaacct ttgaaagttc actttcttca tcaagataca
     1801 ccgtcgtttc ctgcgcatat gatgtcttta aaagcaaagc atatatctcg tcaaaagtat
     1861 aaactccgtc tattatcttc tgataatact gcttagcttc atcattttta tcaaaataaa
     1921 gaagctttat tgatgcataa ggtcttgata cataactttt cttgttagta tcgtactcct
     1981 ttttcatttc cgaatcggta aagctgtaag aaagagttat cttttcatgc agctttaaga
     2041 ttgcattttt ataaagagcc gaataaaatt tatcgtttat ataacttgct atgttaagat
     2101 agcctttggt aaaaaggtac atatcaatat cttcctgtgt aaaacttgaa tccttgaaaa
     2161 tctgttctat attctgcttg aaataatcct tggtggttgt cctctgaaga tcaataccct
     2221 ttgactcaac aaactgaata aaaagaactt catttatcag cttatctctt acatattctg
     2281 agtactcatt caaaagttca atacctgtag ccgtctttgt aagcgtgtca taaaaatcag
     2341 gaaagttaag ctttaacgac tgtaagataa ccaagctttg acttcttgct gtaagcagat
     2401 cattgtcaac tgtttcgccg tttgctttaa acgctatgtt ctgtccaaga tctccgtatg
     2461 tgaccacagc aaaaagacct actgacaaca ccgtaacaaa taccagaaaa aaaagttttt
     2521 tcatcctttt tcacccctta ttttatccca cattttgatt ttatcttctt cggagtactc
     2581 gttgaacttt tcgcccgcaa gtttttccag agaaacaaat ctttcatgaa actctttgga
     2641 ggcttggtta acaagacttt caagattaat gctttccttt acactcatat agattatttt
     2701 ttccagtact cctttataat ttccggaata aagatcttca cttataagtt tttttacatc
     2761 ataaccgaag taaggatcaa agcctacagc tagcgaattc tcaaaaagcc ttcttaattg
     2821 gattatggga ggagctcctc tcttatactt gcctattctg cacattttct cattatcttc
     2881 ttttgacttc agcatttccc actgctcata agaatagcct ttaccatcag aaaaaacatg
     2941 cggatgcctt ctgaccatct tgtcgctgac tgaacagagc acatccccga tatcgaactc
     3001 tttatcttca taggctatct gtgcatgaaa aatacattga aacatgaggt cgccgagttc
     3061 ttttttcaac tcggaagtat ctccgctgtc tatgctttca acaacttcat atgcctcttc
     3121 tatcgcataa ggcctaagag actgatgagt ttgcttttta tcccactcac aacctttttc
     3181 agatctgagc tgttccatta tctctaaaag ccttataaat tctgatgaat atacttcctt
     3241 tcttttctca tccatacttg tttctccctt agtcatacat tccgtaataa ccggcgatct
     3301 ttttgagaag acctccagcg gcatcattgt ttttttcgtt actgtataca agttttatat
     3361 actcctgagc ctgaaaactt tcaagtactt cgtctgcagt tatagctatc atttggcctt
     3421 gcggcgaata acctataaat gcacatatat gagctgcgtc atcaaaaata cctgaacctc
     3481 caagcaaagc ttcttcactg ataatgtcac ggtattcata tgtaaaaaag tttacatacc
     3541 ctgtttttga ctctgaaagt ccaagcttga tagatctttg tccaataaaa tcataaagct
     3601 catatgcatt ggattgtgaa aaagccagag aagccggcga aatatcctgt ttgatcctgt
     3661 ctttagatac tttcattata caaagatcct taagctttct gtttatataa atactgtcag
     3721 gtgcaactct ttgatctcct actttgagcc ttacatccat atatgaaaag ttttgctcat
     3781 taagcttggt cattaccgga attattccgc atgctgaaag ggatgtcagt atatacaccg
     3841 actccccgtc ttcatagaaa atacttcccg atgcagagaa aacatactct tcattatcag
     3901 aaaacctgaa aaaggacgat attgccactg aacagctcct gatatccgaa gcttcaaaag
     3961 tctcaaattc ttgagcaact ttaaggggcg tatttatgta actgacataa cttccaaaat
     4021 gatcgccgtt tggttcggtc catggcgtaa gtctgctata ctcgccaatc tgcattgctt
     4081 ttacatcacc gctttcacac gggattaaaa gctcagctac cccatcgata tccacatcat
     4141 caataacaac attcgcagaa caggtatcaa ttatgtcggc tttaaacttt aactcatcgg
     4201 taagagtatc gtacctgtat acttttccgg atgaagaaac caaaaagatc ttattgctcc
     4261 atgcagtttc gttatcatac agccctatca ccagtggtga agaaaaaaat gcttccgaag
     4321 gaatctcttt ctgcttgaca aggtttccgt ccggatcaaa tatgctcatc tttcccgatt
     4381 catccgcagt aattatgttg attattcctg acaagtaaag atcacttgca gcaggatttg
     4441 aaagaactct gcttttaagc tcacttataa gccttgcatt tccaaactta tcactataga
     4501 taacctttcc gctcaaggta cagtaaacta tatcaaaatc accatcacta tttatgtcac
     4561 atatcaaagg caacgaaaaa atatcgtcac ccagttcaaa ctgccacagt atttcaccac
     4621 tggcatccaa aagagtcgat actccctcaa aagatgtcat gagaagctca tactttccat
     4681 catagtttat atctacaggc tgcgggggca cagaataata ctcataacct atataaactc
     4741 tcgatactat gttacccaga taatcccaaa gataaataaa accattcatt gaagctgaaa
     4801 taacatatct gtcgctttgc cattctgcac tgtcacaaaa gaaagagctg ggagccatga
     4861 aataatcttc gttttctgtc aattctactg acgatctctt gatttttgag tttttatcca
     4921 gaacattaac ggtaacgtac tcgccttttt tcatatcctt tagtatctgc attatatccc
     4981 accagctgag aacatcacgt ccctgaacct taagtattct gtccccggct gaaaaaacat
     5041 ccatgtagtc atcggcacac actatacttc cgtcctgtat ggtcacatta aacggaagat
     5101 ccgtaaagac aggagaaaaa gaagtagtca tctttaaagt gcctccgatg actttaaaaa
     5161 cttgcaccgg gccttgagcc agcactgcca ctatttcact ctcaccatca ttatctatat
     5221 ctttacaaaa acatggaaaa acaggcgact ctgtttgaaa cgagtatctg gtatgaccgt
     5281 ccgtcctgtc aataatcttc aaagaaccgg attcttgtac ctctgaaaat gaagatccga
     5341 aaacatagct gtttttatca taaaaccgtg gaatgctcct aataaaatga tcctgctttc
     5401 ctgcttggcc atcccagaga ataattgaaa aaacactgca tgcacaaata ataattccga
     5461 cacaaaaaaa tactctcttc atacttaccc cctatcacat acctatcctc aaaagtctct
     5521 gagctgagaa ttccgaccta tcggcaatta tttgtccgtt tacaatcaaa gttctgacag
     5581 gatcaaaagt aattctgaat tcattaaaga ttccatattt ttttaccatc tgctcatgaa
     5641 gcttagtcgg atagccttta tgcttttcaa aaccatactg agaatatttt tg
//
LOCUS       DAOSHR010000132         5677 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_12563_length_5677_cov_6.144255, whole genome shotgun sequence.
ACCESSION   DAOSHR010000132 DAOSHR010000000
VERSION     DAOSHR010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 5677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5677
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12563_length_5677_cov_6.144255"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="PLU28_07755"
     CDS             complement(<1..474)
                     /locus_tag="PLU28_07755"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HOG34994.1"
                     /translation="MNIQQLIMALDKFWSDNGCVIDQPYDIEMGAGTYHPSTFFRSLD
                     PFEWKVAYIQPSRRPKDGRYGENPNRMQRFYQYQVLIKPSPENIQELYLQSLKTLGID
                     PMEHDIRFVEDNWESPTLGAWGVGWEVWLDGMEITQFTYFQQMGGIDVKPVSVEIT"
     gene            complement(517..2199)
                     /locus_tag="PLU28_07760"
     CDS             complement(517..2199)
                     /locus_tag="PLU28_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HOG34995.1"
                     /translation="MKKKLYLDGKDIYIGIKKGTEVLIKNKDEINALNVFPVPDGDTG
                     SNMSAAMLEACQWLDKVKDPTVTKDILKALKDGLLMGARGNSGVILSQIFRGFTEAIE
                     TKKKVNTNDFILALKRAKEVSYNAVIRPVEGTLLTLIRRLSERSEKELSEIEDFVEFM
                     DKLYIMSDEIVRETPKYLKKLRDANVVDAGAKGFSYVLKGFSDSLKGDTQVNLLKIET
                     ASAQEIASFAYEDIKYQYCTEVILNLKDAKIPKTDLEKLRAFLEENGDSIVLVHQNEF
                     LKCHVHSNHPGIVFEKFLEFGELMKVKVENMKVQHEHLIDDKNKTEEMEVQQQEEETE
                     TETEVFPQRNWGIVSVSPGKGLSEIFKSLGVNKIIFGGQTMNPSVKDIADALNAMPYS
                     KAIVLPNNPNIIMTAQKAAEMSDKEVLVLPTKYVQEGVAALLGFQDEMERQDLEQSMQ
                     GYIDSIVPIQVTYAVRDSEIGSEKIKKNENLVFVGKDLKAHGKDIYKETLKALTEVID
                     QGYEIVTIFYGEGVEEKHINQFVKRLTDKYSKIEVEVHYGGQQHYPMLISVE"
     gene            complement(2238..2579)
                     /locus_tag="PLU28_07765"
     CDS             complement(2238..2579)
                     /locus_tag="PLU28_07765"
                     /inference="COORDINATES: protein motif:HMM:NF015721.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HOG34996.1"
                     /translation="MTLKTEYGTLVIKPEVIRDIVYKAVSESYGTVDTGKQGFFNKIA
                     NLLAKEEAKGINVEESDRGIFVSVDLVLEYGLPIKKVAENAQENIHHRVTSMLNFQDV
                     VVNVHIAGLKS"
     gene            complement(2609..3421)
                     /gene="amrB"
                     /locus_tag="PLU28_07770"
     CDS             complement(2609..3421)
                     /gene="amrB"
                     /locus_tag="PLU28_07770"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOG34997.1"
                     /translation="MYSKKRMPAASGKFYPQDSSELSAKMKGYFPEGFHAGEKLIRCS
                     GAIVPHAGYVFCAQTAAKAYEKIFKDGTVDTVVIIGPNHSGLGESISVYPQGSWVTPL
                     GEVEVNSTLASDILSKLSRNGDYLAHIYEHSLEVQLPFIQYLTGNNFKILPICMKDQS
                     IEAASALSGILKEYKHDGVLFIASTDLNHYESQEVTLKKDAMVMDAVIKMDMQELYSS
                     LKHYDISMCGYGPVAVLMGLEFEKSVLTGHNTSGDVLDEYTSVVGYLSAVLT"
     gene            complement(3444..>4424)
                     /locus_tag="PLU28_07775"
     CDS             complement(3444..>4424)
                     /locus_tag="PLU28_07775"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34998.1"
                     /translation="KVIKVPLKVKDIFDRIIIKQGVKLTEISRTDSDIFYLIEYDESC
                     EEFVLEYIQSVLSDNSGVSQSSDIKELIVSVCRRNGLNCVCDLSKEIKIHVNHDAFTM
                     ESLFRLCFAKGISYNYLDNQTIRFFNKQKMLKYEIIVMNGSDLGSITLENLYEISRLN
                     ITSSRYIDEKLNILARQSFYVMQGEEAQINSNVSIPVLVDEKDGKKVYSKVQSSFKSR
                     VKGSYDEMTGSITTTFYVELTEAKEQKESALDSKTIETSFMTGNGGLIKIGGLNFSKT
                     YRMKKGVPLLKNIPLIGDFFSYYKNEVSSFELVFLIRAEVLTKPVAQDYK"
     assembly_gap    4425..4524
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4525..>5677)
                     /locus_tag="PLU28_07780"
     CDS             complement(<4525..>5677)
                     /locus_tag="PLU28_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG34999.1"
                     /translation="WIRMEPFPQEKLSLWSDEIFFEDFIRLMEKTYAVQVVFINQDTF
                     VAGKNAAEFENKLPYFIKEGVAQKSIFDIQSTIAGTLTQIPTDTVYLKSTLSEILSSA
                     IISSRGEKISVPASGTDADKDVTRRTGDLIQYRDRILQEFLRQEVLDMPGHDFEKYEL
                     VSKEETSVQTGPAGKKEKPEEYELYEKKPEKPSQNLTTEKKSVEEEKFFYFFNSRYDL
                     GQLEKFFDCKFQRFDDYFLTYTSALNIAQIIRIQDFLNDAYRAKSREKDRQDEEIQKI
                     HESYAASITYLQEQEKRLKESIQQAPDSKLSKDATAVDPQPTFSVEEPLLIESSNEST
                     AQAYNDEILVEPQKSSQEIKEEDGKNITKRLNKVIKVPLKVKDIFDRIII"
BASE COUNT         1495 a         1262 c          904 g         1916 t
ORIGIN      
        1 ggttatttca acagaaacag gttttacatc aattcctccc atctgctgaa agtatgtaaa
       61 ctgagttatt tccattccgt caagccatac ttcccatcct acaccccatg ctcccagagt
      121 aggtgactcc cagttatcct caacaaatct tatatcatgc tccatcgggt ctattcctaa
      181 tgttttaagc gactggagat aaagctcctg tatgttttca ggtgacggct ttataagaac
      241 ctgatattga taaaaacgct gcatcctgtt gggattttct ccgtaccttc cgtctttcgg
      301 ccttctgctt ggctgtatat atgcaacttt ccattcaaaa ggatcaagag acctgaaaaa
      361 tgtggacgga tgatatgttc ccgctcccat ttctatatca tacggctgat cgataacaca
      421 accattgtcg ctccaaaact tatcaagcgc cataataagc tgctgtatat tcaaaatatt
      481 ccctccagat caaaaagatt gtaattattt gtccgattat tcaaccgata taagcatagg
      541 atagtgctgc tgaccaccgt aatgaacttc aacttctatc ttgctgtact tgtcggtcaa
      601 tcttttgaca aattggttta tatgtttttc ctctacacct tcaccgtaga agatagtaac
      661 tatttcatac ccctgatcaa ttacctctgt aagtgctttt aacgtttctt tatatatatc
      721 ttttccatgt gctttgagat ctttacccac aaaaaccagg ttttcattct tcttaatctt
      781 ttcactgcct atctcagaat ctcttacggc atacgtaacc tgtattggaa ctatagaatc
      841 aatgtacccc tgcatggatt gttcaagatc ctgtctttcc atctcgtcct gaaagcccag
      901 cagagccgcc acgccttcct gaacatactt ggtgggaagt actaagactt ccttatcgct
      961 catttctgcc gctttctgtg cggtcattat tatattggga ttatttggaa gaactattgc
     1021 tttcgagtag ggcattgcat tcaaagcgtc ggctatatcc ttaacgcttg gattcatagt
     1081 ctgtccgccg aatatgattt tgtttacccc caggctcttg aatatttcac tgagtccttt
     1141 accgggtgaa actgaaacga taccccagtt tctctgaggg aacacttctg tttctgtttc
     1201 tgtttcttct tcctgctgct gaacttccat ctcttctgtt ttattcttat catcaatgag
     1261 atgctcgtgc tgtactttca tattttcaac tttaaccttc atcagttcgc caaactcaag
     1321 gaacttctca aaaactatgc ctggatgatt tgagtgcaca tggcatttaa ggaattcatt
     1381 ctggtgaaca agtactatgg aatcaccgtt ttcttcaaga aatgctctta gtttttcaag
     1441 atcagtcttt ggtattttag catctttaag gtttagtatg acttcggtac agtactgata
     1501 ctttatgtcc tcataggcga aggatgcaat ctcctgcgca gacgctgttt ctatcttaag
     1561 aaggtttacc tgagtatctc ccttaagaga gtcgctgaaa cctttaagca catatgaaaa
     1621 accttttgct ccagcatcca caacgtttgc atcccttaac ttttttaggt atttcggagt
     1681 ttctcttaca atttcatcgg acattatata gagcttatcc atgaactcaa caaagtcttc
     1741 tatttcagaa agctcttttt ccgaacgttc cgaaagcctt cttataagag ttagaagcgt
     1801 tccttctacg ggccttatta cagcattgta tgaaacctct ttggcccttt ttagtgcaag
     1861 aataaaatca ttcgtattta ctttcttctt ggtctctatg gcttcggtga atcccctgaa
     1921 tatctgggaa agtataactc ctgaatttcc tcttgcaccc atgagcagtc cgtctttcag
     1981 agcctttaaa atatcttttg tgaccgtagg atcttttacc ttgtccagcc actggcacgc
     2041 ctccagcata gctgcactca tgtttgaacc tgtatctccg tcaggaaccg gaaaaacatt
     2101 caatgcattg atctcgtctt tattctttat aagcacctcg gtaccttttt ttatgcctat
     2161 atatatatct ttaccgtcaa gataaagctt tttcttcaat cttctcacct ctacaagtcg
     2221 ctgaataaat tatgtgccta tgatttcaaa cctgctatat gaacattcac caccacatcc
     2281 tgaaaattca gcatggaagt tactctgtgg tgtatatttt cctgggcatt ttctgcaact
     2341 tttttaatag ggagcccgta ttcaagaaca aggtctacag acacaaatat gcctctgtca
     2401 ctttcttcta cgtttattcc cttagcttct tctttggcca gaagattggc tatcttattg
     2461 aaaaaaccct gttttccggt atctacagta ccgtatgatt cagaaactgc tttataaact
     2521 atatccctta taacctcagg ttttataacg agagtgccat attcggtttt taatgtcata
     2581 tcatacacct cctggcagaa tttttatttc atgttaatac cgctgacaga taacctacga
     2641 cactagtata ttcatccagg acatcaccgc ttgtattgtg ccccgttaga acagattttt
     2701 caaactcaag tcccatcagc acggctaccg gcccatatcc acacatgctg atatcataat
     2761 gctttaacga actgtaaagc tcctgcatat ccatctttat tacagcatcc ataaccattg
     2821 catctttttt taaagtaact tcctggcttt catagtgatt aaggtcggtc gatgctataa
     2881 aaagaacacc gtcatgcttg tattccttaa gaatacctga aagtgcggat gctgcctcta
     2941 tactctgatc cttcatacat atgggaagta ttttaaagtt atttcccgtc aagtactgta
     3001 taaatggcaa ctgtacttcg agcgaatgct catagatatg cgcaaggtaa tcgccgttcc
     3061 tactcaattt tgaaagtata tcagatgcaa gtgtgctgtt tacttcgact tctcccaaag
     3121 gagtaaccca gcttccctgc ggataaacag aaatactttc tcccaacccg ctatgattgg
     3181 gtcctataat aacaactgta tctaccgttc cgtccttaaa aatcttctca taagcttttg
     3241 cagctgtctg cgcacaaaaa acatacccgg cgtgaggcac tatggcacca ctgcatctta
     3301 tgagcttttc accggcatga aaaccctcgg ggaagtatcc tttcatcttc gctgaaagtt
     3361 ctgatgaatc ctgcgggtaa aactttccgc ttgctgcagg cattcttttt tttgaataca
     3421 tgctgcaccc cctgtcattt caatcatttg taatcctgtg caaccggttt ggtaagaacc
     3481 tccgccctta taagaaaaac gagttcaaaa cttgaaactt catttttata gtatgagaaa
     3541 aaatctccta ttaatgggat gtttttcaaa agtggaactc cttttttcat cctgtaggtt
     3601 ttggaaaaat taagtccgcc aatcttaata agaccgccgt ttccggtcat gaaggaagtt
     3661 tctatggtct tgctgtcaag agcgctttct ttttgttctt tcgcttcggt caattcaaca
     3721 tagaaggttg ttgttatact tcctgtcatc tcgtcataac ttccctttac cctggatttg
     3781 aaactagact gaactttgga gtaaactttt tttccgtcct tttcatccac cagtacaggt
     3841 atggatacat ttgaatttat ctgagcctct tccccctgca taacataaaa gctttgtctt
     3901 gcaagtatat taagcttctc atcaatatac cttgagctgg ttatattcaa ccgggatatc
     3961 tcataaaggt tctcaagagt tattgacccc aagtcacttc cgttcattac tattatctca
     4021 tatttaagca ttttttgttt attaaaaaaa cggatagttt ggttatccaa ataattataa
     4081 cttatacctt ttgcaaaaca tagccgaaac agtgattcca tagtaaaagc atcatggttg
     4141 acatgaattt ttatttcctt ggaaagatca catacgcaat taagtccatt gcgccggcat
     4201 acggagacga taagctcctt aatatctgaa gattgcgata ctccagaatt atctgacagc
     4261 acagactgaa tatattcaag tacaaactcc tcacaacttt cgtcatattc gatcagataa
     4321 aaaatatcgg aatcagttct cgaaatttca gtaagcttca ccccctgctt tataattatt
     4381 ctatcaaata tatctttaac tttaagaggc acttttatca ctttnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnttataa ttattctatc aaatatatct ttaactttaa
     4561 gaggcacttt tatcactttg tttaaccttt tggttatatt ttttccatcc tcttctttta
     4621 tttcttgcga tgatttttgt ggttctacca aaatttcatc attataagcc tgtgctgttg
     4681 actcattgga agattctatc aacaacggtt cttctacaga aaaagttggt tgcggatcaa
     4741 cagcagtggc atctttggaa agcttgctgt caggagcttg ctgaatactt tcttttagcc
     4801 ttttttcctg ttcctgcaga taggtgatgc ttgcagcgta actttcgtgt atcttttgta
     4861 tctcttcgtc ctgtctatcc ttctctctgc tcttagctct ataagcatcg tttaaaaaat
     4921 cctgaattct tattatctgc gctatgttca aagcagaagt atacgtcaga aaataatcat
     4981 caaatctctg aaatttgcag tcaaaaaatt tttccagctg tccaagatca tacctgctgt
     5041 taaaaaaata aaaaaacttt tcctcctcta cagatttttt ttccgtagtt aaattctgac
     5101 taggtttttc cggctttttt tcatacagtt catactcttc aggcttttcc ttttttccag
     5161 caggacctgt ctgtacactc gtttcttctt tggatacaag ttcgtacttt tcaaaatcat
     5221 gcccgggcat gtccagaact tcctgcctta aaaactcttg aagtatccga tcgcggtatt
     5281 gaataagatc tccggttctg cgggttacat ctttgtctgc gtcggtacca gacgccggaa
     5341 cagatatttt ttctcctctg gaactgataa tcgcactgga caaaatctcg gaaagagtcg
     5401 acttaagata taccgtatct gtgggaatct gagttaaagt tccggcaata gtgctttgga
     5461 tatcaaagat agacttctgt gcaactcctt cttttatgaa ataaggaagc ttgttttcaa
     5521 actctgccgc atttttcccg gcaacaaagg tatcttggtt tataaaaaca acctgaacgg
     5581 catatgtttt ttccataagc cttataaaat cttcaaaaaa aatctcatct gaccacaaac
     5641 tcaatttttc ctgaggaaat ggctccatcc ttatcca
//
LOCUS       DAOSHR010000133         5666 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_12594_length_5666_cov_5.612369, whole genome shotgun sequence.
ACCESSION   DAOSHR010000133 DAOSHR010000000
VERSION     DAOSHR010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 5666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5666
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12594_length_5666_cov_5.612369"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..859)
                     /locus_tag="PLU28_07785"
     CDS             complement(2..859)
                     /locus_tag="PLU28_07785"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HOG35000.1"
                     /translation="MNKKVLKEYIVSFFGTFLTAVGLVSFLMPNKIATGGANGIAIII
                     NGLTGFPVGIIMYAVNIILFILSFILIGKAFGLRSIICTFVLNFFVDFLDRMVPFPKF
                     TTDDYFLAVFLGVFISALGMAIVFSVNSSTGGTDILAKIASKYTGVSLGINLLFIDLT
                     IGIMAGGVFNMKVGMYSVVSVILNGIVVDYAVRMIKSNCILTIITDHREETLDFIMKN
                     LDRGASIIKATGAYTQQEKNMIYVAVNKKQKGEVVRKIHTIDPKAFVIVQESSDVIGY
                     GFSDFQNFF"
     regulatory      complement(966..1143)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            1287..>1762
                     /locus_tag="PLU28_07790"
     CDS             1287..>1762
                     /locus_tag="PLU28_07790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis isomerase"
                     /protein_id="HOG35001.1"
                     /translation="MKIKVFKMSSFTESISGGNPAGVVMEGLLSESQMKKTAAVVNCS
                     ETAFVSESDEADFKVRFFTPVSEVDLCGHATIATFALMFLKKKLQAGRYSQQTKAGVL
                     GIEIHPDGQIMMEQALPQFGEEIDKDEVAKVFGIDKKAFSDELPIQVVSTGLRDIIV"
     assembly_gap    1763..1862
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2009..3409)
                     /locus_tag="PLU28_07795"
     CDS             complement(2009..3409)
                     /locus_tag="PLU28_07795"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOG35002.1"
                     /translation="MVLKISGMIDDGSLAAGFRLPSSRTLAQTLRVSRTSVVAAYEEL
                     WSCGYIDTRQSAYSYVRDINTNPSFSMNDEILSDEKLNSSRIDFLKKEKSLYYNSSCI
                     DMMSLFPDERTYPVKEMKKCYRKVLERYGEKLFTYADKQGYQPLRLYLSQRLKTIGIK
                     VDAQDILITNGTQNSLDLIMRMFKKPHQRIAFESPTYANFLSLLKLHGYEYFEVPLKN
                     KQFDYKTFEKEVITNNNFSFIYLIPDFQNPTGLTMSKNERNNILQVCKENDIFIVEDA
                     YEEEMKYLGKIPLPLKSYDSAGNVIYCGSFSKILFPGIRISWIAAKKSIIEKLTYIKR
                     LSDLSSNLINQVAIYEFCKNGYYEKHINQMHRIYKKRMAFFINHIEKHVQPGKIELIV
                     PEGGYTLWISIIKSRFSADEVYALFVNKGLMVSPGKIFFHDDSDSLSFRLSVARLNEE
                     ELEAAAKILSEVLEDV"
     gene            complement(3469..4215)
                     /locus_tag="PLU28_07800"
     CDS             complement(3469..4215)
                     /locus_tag="PLU28_07800"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOG35003.1"
                     /translation="MIELIKQNLFSFYRIGIKERSRNDYKMLDYSYAGERWPTRVWIK
                     NKEVYDEEELELFFSDDYFKGKNISWFLENCSTGNQIRDFVIKKGYTFLYESAGMIKD
                     CQDFIFSPYADLSAEKVITEYKLEIFKEIVSKGLFERNPGEISESIFMNLFKNKNIEL
                     YLLSEKNNYIGTGGIFLDSSKSGGIYMICIDKDFRKRGYGKYITSLCMNRIRDLGYRY
                     CVLNATLLGEPIYKELGFVSPVSFYILRKK"
     gene            complement(4341..4751)
                     /locus_tag="PLU28_07805"
     CDS             complement(4341..4751)
                     /locus_tag="PLU28_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35004.1"
                     /translation="MSQTAEFLGWLNVSLVIINTMLYLTRLIYKKIVSKKNNGFWITY
                     KKMMAVLKKVHIFSGAAVIILGLVHGLMMLGGSLYIHTGLLVWINILFLFGIFALSKL
                     SKTFKKIWILPHRLLAITLWGLLILHLIKPWLLN"
     gene            complement(4762..>5666)
                     /locus_tag="PLU28_07810"
     CDS             complement(4762..>5666)
                     /locus_tag="PLU28_07810"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOG35005.1"
                     /translation="TAELDSSSVILKDKSQANIELSQKLKESLNMIQKNTENVAASVE
                     ETTASIEDVSSGAKLISTSALELNSKTDEIVNKIGASKNEITVMLENADKIKNLMSSN
                     NQNIQELSGKTESIKDITQSIHSIAEQTNLLALNAAIEAARAGDAGRGFAVVAEEVRK
                     LAEESKVAAAKIEDNIEYLVNDSMEVAKESEGVTNNVIGIVSSIKNIGKSLEEVLESI
                     SMISSMIDNTTSSAQNQGENIEQVGNAMNTINTAVANIVAEIDSINQEIDTQSDIIKE
                     VANISERINGTVLKLSEFEKKFRL"
BASE COUNT         1600 a         1027 c         1011 g         1928 t
ORIGIN      
        1 tctaaaagaa gttttgaaag tctgaaaatc cgtatcctat tacatccgag ctttcttgga
       61 caattacaaa ggctttggga tctatggtat gtatttttct tacgacctcg cccttctgtt
      121 ttttattcac tgcaacatag atcatattct tttcctgctg ggtatatgct cctgttgcct
      181 ttattattga agctcctctg tccaagtttt tcattataaa atcaagggtt tcttctctat
      241 ggtctgttat tatggtaaga atgcagttgg attttatcat ccttacagca taatctacga
      301 ctatgccgtt gagtattaca gatacaacag agtacatgcc tactttcata ttaaagacac
      361 cgcctgccat aattcctatg gtcaggtcaa tgaagagcag gttgattcca agagatacgc
      421 ccgtatactt tgaagctatt ttggcaagta tatctgttcc ccctgtggat gagtttacag
      481 aaaaaactat ggccatgccc agtgcagaga tgaaaactcc tagaaatact gcaagaaagt
      541 aatcatcggt tgtaaattta gggaacggaa ccatcctgtc aagaaaatct acaaaaaaat
      601 tcagcacgaa ggtgcatatt atggatctga gtccaaaagc ttttcctatt agtatgaagg
      661 acaaaataaa aagtatgatg tttaccgcat acattatgat tcctacagga aagcccgtaa
      721 gtccgttaat gattatcgct attccgtttg ccccgcctgt tgctattttg ttgggcatga
      781 ggaaagaaac cagtcctacg gcagtgagaa aagttccaaa aaaagaaacg atgtactctt
      841 tcaaaacctt cttattcaaa gagatcccct ccatgttaaa aaatctttct tatccgtgag
      901 caggaaaaga aagatttttt gtatataaat acatattatc tttttacttt ccaatccagg
      961 agaaagcagt aaaatatatt tcaggcagtt cttctgactt acagcttaac ctactcgtct
     1021 gccttcccga tcttgcgatc agtgacttcc tgactttcgt atctgtttac agcggccgga
     1081 ccgttccgga ttctcaccgg attatctatt aagactggta tcacctgaaa tggttattta
     1141 cttttctaaa agtattctat cataaacctt atggttttaa aagacagaat aaatgaagtc
     1201 agcgttctgt gtaaaggtat agttccaatg gcatcatttt taggtatact ttctgtagaa
     1261 agtcatttta ataaggggga gtagcaatga agatcaaagt ttttaagatg tcatcattta
     1321 cggaaagcat atctggagga aatcctgcag gggtagttat ggaaggtctg ttaagcgagt
     1381 cacagatgaa aaagacagcg gccgttgtta actgctctga gacagcgttt gtgtcggagt
     1441 cagatgaagc agattttaaa gtacgttttt ttactccggt gtctgaggtg gatctttgtg
     1501 gacatgcaac tatagcgacg tttgctctta tgtttttaaa aaagaagctt caagccggaa
     1561 ggtactctca gcagacaaag gctggggttc tgggtataga gattcatccg gacggtcaga
     1621 taatgatgga gcaggcatta cctcaatttg gagaagaaat tgacaaagat gaggtagcta
     1681 aggtttttgg catagataaa aaagcgtttt cggatgaact tcctattcag gtggtttcaa
     1741 ccggtctgag agatatcatc gtnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnggttggag ggaaagcagt ctttttagaa caggttgaag ttgaaatata aaagttactt
     1921 attttttctt tacaaaaagt gttttgtgtg gtataattta actgtacaat taaaactccg
     1981 gcttgccgga gtttgttttt ttaattcttc acacatcttc aagtacttcg gataaaattt
     2041 ttgctgcagc ttccagttct tcttcattaa gtctggcgac agaaagtctg aaactaagag
     2101 agtcggagtc atcatggaaa aatatttttc cgggggatac cattaatcct ttattaacaa
     2161 ataaagcata aacctcatcg gctgagaatc tactttttat aatacttatc cataatgtgt
     2221 atcctccttc cggtacaatt aattctattt ttccaggctg aacatgtttt tctatatggt
     2281 ttataaaaaa ggccattctt tttttatata tcctgtgcat ttgattaata tgtttttcat
     2341 agtagccgtt tttacaaaac tcatagatcg caacttggtt tataagatta gaagaaaggt
     2401 ctgaaagcct ttttatatag gtcagttttt cgataatact tttttttgcg gcaatccatg
     2461 agatacggat acccggaaag aggatttttg aaaaacttcc acagtagata acattcccgg
     2521 cgctatcgta tgacttcaaa gggagcggaa tctttcctaa atacttcatt tcttcttcgt
     2581 aggcatcttc tacgataaaa atatcgtttt ctttacaaac ttgcagaata ttatttcttt
     2641 cgttttttga catagtaaga cctgtagggt tttgaaaatc aggtataagg taaataaaag
     2701 aaaaattatt gtttgttatt acttcctttt caaaggtttt atagtcgaat tgtttatttt
     2761 tcaaaggaac ttcaaagtat tcatatccat ggagcttcaa aagagataga aaatttgcat
     2821 aggtaggcga ttcaaaggct atacgctgat gcggtttttt aaacatcctc attataagat
     2881 cgagagaatt ttgtgttcca tttgttatga gtatatcctg tgcatctact tttattccaa
     2941 tggttttcag cctctgtgat aaataaagtc taagaggctg atatccctgt ttgtctgcat
     3001 aggtgaaaag tttttctcca tacctttcaa gaactttccg ataacatttt ttcatctctt
     3061 taacaggata agttctttca tccggaaaaa gcgacatcat atctatgcag gagctgttgt
     3121 agtaaagaga tttttctttt tttaaaaaat ctatccttga agaattaagt ttttcatccg
     3181 acagtatctc atcattcatt gagaatgaag gattggtatt gatatctctg acatatgagt
     3241 atgcgctctg cctggtatct atgtatccgc atgaccaaag ttcttcatat gcggctacca
     3301 cagatgttct gctaactctt agagtttgtg cgagggtacg agatgacggc aatctgaaac
     3361 ctgccgccag acttccgtca tctatcatac ctgaaatttt taagactatc tgtttgtata
     3421 aaggtgtttt tgaactttta tcaatgtcaa aaaaaatatt tatccctcct attttttccg
     3481 caggatgtaa aagcttacag gcgaaacaaa gcccagttcc ttatagatag gttctcccaa
     3541 aagagtcgca tttaatacgc agtatctgta acccagatct ctaatcctgt tcatgcacaa
     3601 ggatgttata tacttgccgt atccacgttt tctgaaatct ttgtctatac agatcatata
     3661 aattccaccg ctcttggagg aatcaagaaa tattccgccg gttcctatat agttgttttt
     3721 ttctgaaaga agatataact ctatattctt gtttttaaac agattcataa aaatgctttc
     3781 gcttatttcc ccggggtttc tttcaaaaag cccttttgag acaatttctt tgaatatttc
     3841 aagtttgtat tctgtaatca ctttttccgc tgaaagatct gcatatggtg aaaaaatgaa
     3901 atcctgacag tctttgatca ttcctgctga ctcatataaa aaagtatatc cttttttaat
     3961 aacaaaatcg cgaatttgat ttccggtact gcagttttca aggaaccatg agatgttttt
     4021 tcctttgaaa taatcatctg agaaaaaaag ttcaagttct tcttcatcgt atacttcttt
     4081 gttttttatc catacccttg tcggccaacg ttctccggca taggaatagt caagcatttt
     4141 atagtcattc ctgcttcttt ctttaatacc gatcctgtaa aaagaaaaaa gattctgttt
     4201 aataagttct atcatattta tcacctcttc agatatttta tgactgagaa ggtattaaaa
     4261 aaatatccag attatatata ataaattaat accacatttt atataaaaaa atctgccgcc
     4321 ggtatggcag cagagtgtat ttaattcaga agccatggtt ttataagatg aagaatcaac
     4381 agtccccata atgtaatagc taagagtctg tggggaagga tccatatctt tttaaaagtt
     4441 tttgaaagct ttgaaagtgc aaatatgcca aaaaggaaga gtatgtttat ccagacaagc
     4501 aacccggtgt gaatatataa gctaccgcca agcatcatta gtccgtgtac cagtccaagg
     4561 attataacgg cagctccgct gaatatgtgc acttttttta gaactgccat catcttttta
     4621 taggttatcc aaaaaccgtt gtttttttta gatacgatct ttttataaat cagcctggtt
     4681 aagtagagca tagtatttat gatcacaaga gatacattta gccatcctaa aaattcggca
     4741 gtctgcgaca tatgttcccc cttataatct gaattttttc tcgaactcac taagcttaag
     4801 cacagtgccg tttattcttt cggaaatatt ggcgacttct tttattatat ccgactgggt
     4861 atcgatctcc tgatttatgg agtctatctc cgcaactata ttagctactg ctgtatttat
     4921 agtgttcata gcatttccga cctgttctat gttttctccc tggttttgtg cacttgatgt
     4981 tgtgttatct atcatggaag atatcattga gattgattca agcacttctt caaggctttt
     5041 tcctatattt ttaatgcttg atactattcc aattacattg tttgttactc cttcactttc
     5101 ttttgcgacc tccatggagt cgttgaccag gtattcgata ttatcctcaa tctttgccgc
     5161 tgcgactttg ctttcttccg cgagcttacg tacttcttcg gcaacaactg caaaacctct
     5221 cccggcatct cctgctctcg ccgcttcaat agcagcgtta agtgcaagaa ggttagtctg
     5281 ctctgcaata ctgtggatag actgggtgat gtcttttatc gactctgttt ttccggaaag
     5341 ttcctgaatg ttttggttgt ttgatgacat aaggttttta atcttatctg cattttcaag
     5401 cataacggtt atttcattct tgcttgcacc tattttattg acaatctcat ctgttttaga
     5461 gttgagctcc aatgccgagg tgcttataag ctttgcacca gatgaaacat cttctatgct
     5521 tgcagtggtt tcttcaacgg aggctgcgac attctcggta tttttctgaa tcatattcag
     5581 ggactcttta agtttttgcg aaagctctat gtttgcctgc gatttatctt ttaagatgac
     5641 cgaagagctg tcaagctcag ctgttt
//
LOCUS       DAOSHR010000134         5655 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_12631_length_5655_cov_4.596607, whole genome shotgun sequence.
ACCESSION   DAOSHR010000134 DAOSHR010000000
VERSION     DAOSHR010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 5655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5655
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12631_length_5655_cov_4.596607"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..710
                     /locus_tag="PLU28_07815"
     CDS             <1..710
                     /locus_tag="PLU28_07815"
                     /inference="COORDINATES: protein motif:HMM:NF020772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1957 domain-containing protein"
                     /protein_id="HOG35006.1"
                     /translation="HKISGKGVEIDRKDYYDIDEAKQAVKEHAADFLDKKIEQAKRLY
                     GIFDGEVPLITAPFDAELFGHWWYEGPLFLEELFRAMSAQQTVKPATPTSFLEKIDSV
                     QVTTPCQSTWGANGYNEVWLNGSNDWIYRHLHQATEIMVCCAKKYEQPDSLKRRVLNQ
                     MARELLLAQSSDWAFIMTTGTTVEYAVSRTKNHIKRFFDLKGMLETDTVDETFLKYLE
                     WIDKIFEDIDFSVYSKV"
     gene            823..>1916
                     /locus_tag="PLU28_07820"
     CDS             823..>1916
                     /locus_tag="PLU28_07820"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOG35007.1"
                     /translation="MRNWFVKHEKIISIVIVALFIVGIVWWSIAAYISSQPTGSGTSV
                     SSRENAVAVLTKDSTELSYPYWVMSDELDKAYQSQSQQYYQYYGQEIDPVFDSLSFKI
                     SIIDDLMDQKIARYYAEQNKLLPSKKDTEKELNKQVDDYLNQLKENKTNWDQIIQYYG
                     SEKQVRDILVNSIKDSIEIKLVVDRITEKVVSVTKDEAIDYITKNFDQLKTQYEEVKA
                     QHILVTDEATANKIKADILSGAMSFDDAAAKFSIDTSNATSSGELGWFKHGTMVQEFE
                     DASFAATVNELIGPVKSQFGSHLIKVEDKKVFSTPEDVLKYPDIYSQIETTLKQDKFK
                     TWFEAYKADQKFGKVYVDKAIGLAARLNSSV"
     assembly_gap    1917..2016
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2017..2481
                     /locus_tag="PLU28_07825"
     CDS             <2017..2481
                     /locus_tag="PLU28_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35008.1"
                     /translation="QYKKENETKSASITSQRTEVLKELFLQYPSSSRVVSEYYQVNPN
                     DNQVKIQYTRIQLNQLKGYISYIGQENLMAYFSKQINDMLRNVATVVGATDSSVGTKL
                     SALDLGLEIADTLGDKNLKIGYLEQIKAIDPNYITDIDKVIQDTKNATDVIN"
     gene            complement(2628..2701)
                     /locus_tag="PLU28_07830"
     tRNA            complement(2628..2701)
                     /locus_tag="PLU28_07830"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2667..2669),aa:Gln,seq:ttg)
     gene            2769..4430
                     /locus_tag="PLU28_07835"
     CDS             2769..4430
                     /locus_tag="PLU28_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HOG35009.1"
                     /translation="MTLTLRCSKTQQTYLIEKGRPFSELAKKFEKQTNRKVYGVKFNN
                     NIKELFKPVEENGEIEFLDLSTPDGVRIYQRGLLFILFLALKDIDPSEHYSVHYSVGN
                     GLYCEFKNSEASEEKLNALKNTMLQIISQDIKFEKLSIDKYKAMSLFSEQGLHSKALL
                     VKFRKKSTVNVYRAKGYYNYFYGYMPVSSAMIDKFDLQKFNGGFMLLHPNETCPDAVP
                     QYKHFEKLSNTFSEYKNWLKIMEIETVGELNEIILKGKEHSIELIRVAEALHEKKYAM
                     IADEIIKSQKARLILIAGPSSSGKTTSAKRLSLQLKVNGLKPVSISLDDYFVERSQTP
                     RDENGNYDFESINALDLELFNKNLVDLLDGKAIELPKFDFISGTRKPSGKVLKIGKDQ
                     PVIIEGIHGLNEQLTNRVPREYKFKIYVSALTQMSLDDVNRIPTTDTRIIRRIVRDFN
                     TRGHSALSTLKMWPSVRRGEEKNIFPFQEDADVMFNSHLAYELAVLKLFAEPLLLQIE
                     PSCAEFSEAKRLLRFLDYYLPITELEEIPRHSVIREFIGRSTFEY"
     gene            complement(4757..>5655)
                     /locus_tag="PLU28_07840"
     CDS             complement(4757..>5655)
                     /locus_tag="PLU28_07840"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOG35010.1"
                     /translation="SYMKLNYGANVAEITPVEKMLEIYQSSYESANADMARASRIVSD
                     LADDSPYGEIKSKMRSIDKNIYNIQKDWEKAIRKPFLRWVLNSVLVALITSLLTVAMT
                     SVAAYPFSRMRFKGRNQGLLFLMIIQMFPAVMYMVSMYGILKFVGDYVPFIGLDTMGG
                     LIFIYLGGIAYNMWLFKGYYDTIPDSLEESAMIDGATRFQTFIKIVLPLCLPIIAVVT
                     ILTFMGNFNEFVLAKIILQSEDKFTYAVGLQSFTAANGPFETEWGIFTAAALMGAVPM
                     MTIFLLMQKWIVGGLTQGSVKG"
BASE COUNT         1884 a         1053 c         1093 g         1525 t
ORIGIN      
        1 atcataaaat ttctggaaaa ggtgtggaga ttgaccgaaa ggactattac gatatagacg
       61 aagccaaaca ggcagtaaag gagcatgcag ctgattttct cgataaaaaa atagaacagg
      121 ccaaaaggct ttacggtatc tttgacggag aagtgccgct tataactgct ccttttgatg
      181 cagagctttt cggtcactgg tggtatgaag ggccgttatt cctcgaagag ctattcagag
      241 ccatgagcgc tcagcaaaca gttaagcctg caactcccac atcatttttg gaaaaaatag
      301 attcagtaca ggttacaacc ccatgtcagt caacgtgggg agccaacggt tataacgaag
      361 tatggttaaa tggctctaat gactggatat acagacatct tcaccaggca accgaaatta
      421 tggtatgctg tgccaaaaaa tatgagcagc cggactcttt gaaaagaaga gttttgaatc
      481 agatggcaag ggaacttctt cttgcgcagt caagtgactg ggcatttata atgacgacag
      541 gtaccaccgt ggagtatgct gtttccagaa ccaaaaacca tataaaaaga ttttttgatc
      601 tcaaaggcat gctcgaaacc gatactgttg atgaaacctt tttaaagtac ttggaatgga
      661 tagataaaat ttttgaggat atcgattttt cagtttactc aaaggtttaa acaaaaaacc
      721 atgtcgggca gtaatgaatt attgtaacac aattcagaaa cttttatgat aaaatactta
      781 aagcgaatgt gttttaacca aacgaatagg aggagcagaa agatgagaaa ctggtttgtg
      841 aaacacgaaa aaattatatc catagtcata gtagcactct ttatagtagg aatagtatgg
      901 tggtctattg ctgcttatat ttcatctcag ccgacaggtt cgggtacttc tgtaagcagc
      961 agagaaaatg cggttgccgt tcttacaaaa gattctacag agctcagcta tccctactgg
     1021 gtaatgagcg atgagcttga taaggcgtat caatcacagt ctcagcagta ctatcagtac
     1081 tacggtcagg aaatagatcc tgtttttgat tctctctctt ttaaaataag cataatcgat
     1141 gaccttatgg atcagaaaat agccagatat tatgccgaac agaacaagct tcttccttca
     1201 aaaaaagaca ctgaaaaaga acttaacaaa caggttgacg attacttaaa ccagctgaaa
     1261 gaaaacaaaa ccaactggga tcagattata cagtactatg gttctgaaaa gcaggtaaga
     1321 gatatacttg taaattccat aaaagattcg atagaaatta aactcgttgt tgacaggata
     1381 accgaaaaag tcgtatcagt taccaaagac gaggcaattg actatataac aaagaatttt
     1441 gaccagctca agactcagta tgaggaagta aaagcccagc atatccttgt gacagatgag
     1501 gctactgcaa ataaaataaa ggcagacata ctctccggcg ctatgagctt tgatgatgca
     1561 gctgcaaaat tctccataga tacatccaat gcaacatcat ccggtgaact tggttggttt
     1621 aaacacggaa caatggttca ggagtttgaa gatgcttcct ttgccgcaac tgtaaatgaa
     1681 ctgataggcc ctgtaaagtc acagtttggt tcccatctta taaaggttga agacaaaaaa
     1741 gttttcagca cacccgaaga tgtattgaag tatcctgata tatacagtca gatagaaacc
     1801 acattaaagc aggataagtt caagacatgg tttgaagcat ataaagcaga ccaaaagttt
     1861 ggaaaggttt atgttgacaa agccatcggt cttgcagcaa gacttaactc atcagtnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncagt ataaaaagga aaatgaaacc
     2041 aaatcagcat cgataacctc tcaaagaact gaagtgctta aggagctttt ccttcagtat
     2101 ccttcatctt ccagagtcgt ttctgagtac tatcaggtaa atcctaacga taatcaggta
     2161 aaaatacagt ataccaggat acagcttaac cagcttaagg gctatatttc atatatagga
     2221 caggaaaacc ttatggccta cttcagcaag cagataaacg atatgctgag aaatgttgca
     2281 actgtagtag gggcaactga ctcttcagtt ggcacaaagc tctctgctct tgaccttgga
     2341 cttgaaattg ccgatacact tggcgacaag aatcttaaga taggctatct tgaacagata
     2401 aaggccatag atcccaacta tattaccgat atagataagg ttatacaaga cactaaaaac
     2461 gcaacagatg ttattaactg aacgataagg tataaccggg ccttatatgg cccggttttt
     2521 tattttaatg tataatttta aaaaaactaa atctatgata taattaagta catgcaatgc
     2581 agggacaaga taaaaaaccg ggctgaatgc ccggttttaa atcccgatgg ctgggacggt
     2641 aggattcgaa cctacgatgt cggagccaaa gtccgatgcc ttgccgcttg gctacgtccc
     2701 aacaataatt ctgaataatt atagcacaca tataatctct tgtcaaatga aaaggaggaa
     2761 aaagaagtat gactttaact ttaagatgct ctaaaacaca gcagacatac ttgatagaaa
     2821 aaggaagacc tttttcggaa cttgcaaaaa aatttgaaaa acaaaccaac aggaaagtat
     2881 atggagtcaa attcaacaac aacataaaag aactttttaa gcctgttgaa gaaaacgggg
     2941 aaatagaatt tcttgatctg tctacccctg atggcgtgag gatatatcaa agaggcctgc
     3001 tctttatact ttttctagca ttaaaagata ttgacccctc agaacattac agtgtccact
     3061 actctgttgg aaacggactt tattgtgagt tcaagaattc agaagcatcc gaagaaaaac
     3121 ttaatgcgct taaaaacaca atgctgcaaa tcatttctca ggacataaaa tttgaaaaac
     3181 tttccataga caagtataaa gccatgtctc ttttttcaga acagggactc cattccaaag
     3241 cgttactggt taaattccga aaaaagagta ccgtcaatgt ttacagagcc aaaggatatt
     3301 acaactactt ttacggctat atgcctgttt cgtcggccat gatagataag tttgacctac
     3361 aaaaatttaa tggcggtttt atgcttttac acccaaatga aacttgtccc gatgctgttc
     3421 cacagtacaa acattttgaa aagctttcaa atactttttc cgagtataaa aactggctta
     3481 agataatgga gatcgaaacg gtaggtgaac taaacgagat aatacttaaa ggaaaagaac
     3541 acagcattga attaataagg gttgcagagg ctctccatga aaaaaaatat gccatgatag
     3601 cagatgagat aataaaaagc caaaaagcaa gactcatatt gatagcaggt ccgtcgtcct
     3661 ctggaaaaac gacttcagca aaaagacttt ctcttcagct taaggtcaac ggtcttaaac
     3721 cggtttctat ttcgctggac gattactttg tagaaagatc ccagacacct agggatgaaa
     3781 acggcaacta tgattttgag tctataaatg cattagatct ggagcttttc aacaaaaacc
     3841 ttgttgatct tcttgacggc aaagctatag agcttcctaa gtttgatttt ataagcggca
     3901 cacgcaaacc atccggaaag gttcttaaaa taggaaaaga tcaaccggtt ataatagaag
     3961 gaattcacgg actgaatgaa cagcttacca acagagttcc cagagagtac aagtttaaaa
     4021 tatatgtgag tgctcttacc cagatgagtc ttgatgacgt aaacaggatt ccaacgaccg
     4081 acactcgtat aataagacgc atcgtgagag attttaatac aaggggacat tcagcgttgt
     4141 ctacgttgaa aatgtggcca agcgtaagac gcggtgaaga aaaaaatata tttccttttc
     4201 aagaagatgc tgacgttatg ttcaattcgc atctggcata cgaacttgcc gttcttaaac
     4261 tttttgccga accccttctt ttacagatag agccttcctg tgcagagttc tcagaagcca
     4321 agaggctgct ccgctttctt gattactacc ttcccattac cgaactggaa gaaattccaa
     4381 gacactcagt aataagagag tttataggca gaagtacctt tgaatactag atgaattgaa
     4441 taataaaata tatagccaca aaaaataggg acatcaaatg atgtccctat ttgctttgcg
     4501 tataagcttg agacttttga tttttaggta aaagctcaat agcagagaca gaaggccgaa
     4561 gagttaagtc aggctgaagg atactgtctt cctaaaacag atactacaat gttgggataa
     4621 aaatagccag aacgtattca ccaaaaaaac catgctgatt gtttgtaaaa atcaccccgt
     4681 tgttattagc ttaatcagag gactaataag cggggtgatt ttgttatagt tttttataat
     4741 taacttttta tggaaatcat ccttttacgg agccttgtgt aagaccacct actatccact
     4801 tttgcattaa caggaagatg gtcatcatcg gtactgctcc cataagtgca gctgctgtaa
     4861 atatgcccca ctctgtttca aacggtccgt tggctgcggt gaaggactgc agacctactg
     4921 cataggtaaa cttatcttca ctctgaagta ttatcttggc cagtacaaac tcgttgaaat
     4981 tgcccataaa ggttagtatt gttactactg caataatagg caggcagagt ggaagaacga
     5041 tttttatgaa agtctgaaat cttgtggctc cgtctatcat tgcagactcc tcaagggaat
     5101 cgggaatcgt atcgtaatag cctttgaaaa gccacatgtt ataggctatt cctccaaggt
     5161 atatgaaaat gagacctccc attgtatcaa gtccgataaa aggaacgtaa tctcctacga
     5221 acttaagtat tccatacatt gagaccatat acattactgc cgggaacatc tgtattatca
     5281 taagaaacag taacccttgg tttcttcctt tgaaacgcat tcttgagaat ggatatgctg
     5341 caaccgaagt catggcaact gtgagcaacg aagttataag agctaccaaa acagagttga
     5401 gtacccatcg caggaatggt tttcttatag ctttttccca atccttctgt atattgtaaa
     5461 tatttttatc tatactcctc atcttgcttt ttatctcgcc gtaaggagag tcgtctgcca
     5521 gatcacttac tattcttgaa gctcttgcca tatctgcgtt ggcgctttca taagagcttt
     5581 gatatatttc aagcatcttt tctaccggag taatttcggc aacatttgca ccataattta
     5641 gcttcatgta actgg
//
LOCUS       DAOSHR010000135         5648 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_12654_length_5648_cov_5.387806, whole genome shotgun sequence.
ACCESSION   DAOSHR010000135 DAOSHR010000000
VERSION     DAOSHR010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 5648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5648
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12654_length_5648_cov_5.387806"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /gene="plsY"
                     /locus_tag="PLU28_07845"
     CDS             complement(<1..579)
                     /gene="plsY"
                     /locus_tag="PLU28_07845"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HOG35011.1"
                     /translation="MGEYILWIIIGYLCGSIPFSYILPLFRGVDVRKLGSGNVGGTNV
                     LRNMGPVMGALCMILDGAKAFVPVIVAINIYSADSWIVYLAGFFAVLGHNYPVWLKFK
                     GGKGVACTVGSYFGMSPLLGLFFFAVWIPATLITQYVSIASLVALGVDVLIGFIYNYK
                     LGVYGLVLLLLAVYRHRENIKRLLKGQENKTDL"
     gene            complement(586..1968)
                     /gene="der"
                     /locus_tag="PLU28_07850"
     CDS             complement(586..1968)
                     /gene="der"
                     /locus_tag="PLU28_07850"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOG35012.1"
                     /translation="MSGPIVLIVGKPNVGKSTLFNRMIGEKKSIVLDFPGVTRDHVYA
                     EARFCERAFTVIDTCGIFENPTDIIQTQQKKAVLDSVKEADLVLFVVDGKNGLSSEDY
                     HTAQFIRKAGVEVLLVANKAEGYDRYEDSVKPELYSLGLGDPIPVSAEHNKNLEELFE
                     TIIEKLEGKGYNLMYSTEEKNSSDIKVALIGRPNVGKSSLFNMLLGTEKAVVSDIPGT
                     TRDPVDQRVNIKDKNYLFIDTAGLRKKSIIEYGSIEMFSNVRTVKSIEKADVVAVLID
                     ATQGITVQDQKVAGLAEDRGKATVIVLNKWDAAPSELRDKKVLKKAIEKDLYFIDYSP
                     VVMTSCLERTGSADLMAAIDKAYASYTRQIPTSALNAALERFLMVTPPPVRGGKRIKF
                     TFVTQVGVKPPVFTFYVTTQDEVPKNYQQSLRNMIRNYIDPYPGSPLFLKFIYKEEKQ
                     LKSNNKSSRG"
     gene            complement(1989..3602)
                     /locus_tag="PLU28_07855"
     CDS             complement(1989..3602)
                     /locus_tag="PLU28_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOG35013.1"
                     /translation="MEDRKNMSDFEQMLNDNEFNELKKGKVFTGKVFESDTDGIWVAL
                     EGSNGDVFVKREDLTRDISEYKSGEEVTVKIVKTNDEEGLNHASEKQGMFDKLRNEIK
                     EGDVVKVIFKAKNLKGYDVFVNGVLKSFLPGSLSGLRQGDAMPKGLVEVKVIGIRGRS
                     ITVSRKDLIDEKLNKAYEDLRENMVVEGIVESIKEFGAFVRINDSVTALIPKSEATWD
                     RNMPLETVLKVGSKVRGMIIKVDKENRKISLSLKQLTEDPWIKIEEKYPVDCIVRAVV
                     TQILPFGFTVKLEPGVEGLIHESEIFWVKKGRISDVAKVGDRIEVKVLGVDTQNKKLT
                     LSYKQAIGDPWTDIDDKYSENEIYTGYVEKLLPNGAIIKLEEGISGFAHVSELSWNFV
                     DNPQEALKESEKVKVKVLNIDKENRKIKLSIKQAIDNPWKLVSQEVKKGDTVKGKVLR
                     YQGSGAVVLIDKYDVEAFLSVKNASVEKVESIQTILNPGDEVEAKVLKIDFETEERKG
                     NMNISIVDLLRDRENKETQEVLNQVNQEN"
     gene            complement(3626..4483)
                     /gene="ispH"
                     /locus_tag="PLU28_07860"
     CDS             complement(3626..4483)
                     /gene="ispH"
                     /locus_tag="PLU28_07860"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HOG35014.1"
                     /translation="MKVIVSDEIGFCYGVNRAYKECLMLDTSEKKTYIYGLLVHNDQV
                     VGKLQKEGISYFEDINNLPVDSRQSRVIVRAHGIGSHDMSVLHSSFAHVLDMTCPIVS
                     AMVEFAKKMQDSGYYIIAFGKKNHAEMIGLLGSIDPEKAVITDRAVIVQQSRVCILSQ
                     TTMDTQDFKEFLSDIIKKNDFSDIIVKNTICFHTVQREKQAAEIALKADLMLIIGSSN
                     SSNTRKLYDIACRGCSRSYLIQTSEDLKKLDFSQVKTIGVLSGTSAPLWEIEKIIDAV
                     HMVDCGNFS"
     gene            complement(4488..5156)
                     /gene="cmk"
                     /locus_tag="PLU28_07865"
     CDS             complement(4488..5156)
                     /gene="cmk"
                     /locus_tag="PLU28_07865"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOG35015.1"
                     /translation="MKKLFHIAIDGPAGSGKSTVAKEISKRLGILYLDSGALYRMIAY
                     YFQGNGVDLADVLYIQSHLDEISIVYADGHYILNGEVISAQIRKPQSGASASAVSRIG
                     QVREKVNSVIRSISEKCSMVVDGRDIGTVVLKDARYKFFLTASPQERAERRYRELIAK
                     NEDVSFEDVLNEINQRDRADSSRSIAPLKPAPDAVIIDTTGMEIERVCDIIAGYVDSG
                     DIRG"
     gene            complement(5200..5535)
                     /locus_tag="PLU28_07870"
     CDS             complement(5200..5535)
                     /locus_tag="PLU28_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOG35016.1"
                     /translation="MEFVISFKDSDVAFIIKVCGDIDAYHSATFKQKTLEHMPHTDKK
                     IVAFDLSEVPYIDSAGLGSIVSLIKESKKTEKELVLFSLQPQVKKIFEMTKLDKIIRI
                     VDTEEEIKK"
BASE COUNT         1508 a         1365 c          914 g         1861 t
ORIGIN      
        1 aaggtctgtc ttattttcct gccccttcaa aaggcgttta atattttctc tatgcctgta
       61 aacagccaaa agaagaagaa caaggccgta gacgccaagc ttataattat agataaagcc
      121 tataagtaca tccactccaa gggctaccag ggatgcaatc gaaacatact gggttattag
      181 agtggccggt atccatacgg caaaaaagaa cagcccaagc agcggactca ttccaaaata
      241 tgaacctaca gtgcatgcca caccctttcc gcctttgaac ttgagccata caggatagtt
      301 gtgccctaaa acagcaaaaa aaccggcaag ataaactatc cacgaatccg ccgaatagat
      361 atttattgct acaattacag gcacaaaagc ctttgcaccg tctagaatca tacaaagagc
      421 acccattaca ggacccatgt ttcttaatac gttagttccg cccacatttc cgcttccgag
      481 ttttcttaca tcaactcccc tgaaaagcgg aagaatgtag ctgaaaggta ttgaaccgca
      541 gagatagcct ataataatcc agagaatgta ctctcccata aaaactcacc cccgggaaga
      601 tttattatta ctttttaatt gtttttcttc tttatatata aactttaaaa aaagcggaga
      661 accgggatat ggatcaatat aatttcttat catattccta agactctgct ggtagttctt
      721 gggaacttca tcctgtgtgg ttacatagaa tgtaaagaca ggcggtttca caccaacctg
      781 tgttacaaat gtaaacttta ttctctttcc acctcttact ggcgggggcg taaccattaa
      841 aaatctttcc agtgcagcat taagagcact cgtaggaatc tgtctggtat atgaagcata
      901 cgctttgtct atggctgcca taaggtcggc agatcctgtc cgctcaagac acgaggtcat
      961 aactaccggg ctgtagtcta taaagtacaa gtctttttct attgcttttt tcagtacttt
     1021 cttatcccta agctcagagg gtgcggcatc ccatttgttt agaacaatta ccgtcgcctt
     1081 gcccctgtct tctgcaagtc ctgcaacttt ctgatcctgc accgttattc cctgagtggc
     1141 atctataagc actgctacca catcagcctt ttctatggac tttactgttc tgacatttga
     1201 aaacatttcg attgaaccgt attcaattat tgatttcttg cgcagtcccg cggtatcaat
     1261 gaaaagataa ttcttatctt ttatatttac tctctggtca acggggtcac gggttgtgcc
     1321 ggggatatct gaaaccacgg ctttttctgt tccaagaagc atattgaaaa gtgaagactt
     1381 accaacatta ggccgcccta taagagcaac ttttatatca ctgctgtttt tttcttccgt
     1441 actgtacata agattatagc cttttccctc aagcttttct attatagttt caaaaagctc
     1501 ctcaaggttt ttgttatgct ctgctgaaac aggtataggg tctcccaggc caagagagta
     1561 aagctccggt ttaacactgt cttcatacct gtcatatcct tcggccttat tcgcgaccaa
     1621 aagaacttca actcctgcct ttcttataaa ctgtgcggta tgataatcct ccgaagacag
     1681 tccgttttta ccgtctacca caaaaagcac aagatcagcc tctttcacac tgtcaagaac
     1741 agctttcttc tgctgggtct gtattatatc cgtcggattt tcaaatattc cgcaagtatc
     1801 tataacggta aaggcacgct cgcaaaagcg tgcctctgca tatacatgat cccgtgtaac
     1861 accgggaaaa tcaagtacta tagatttttt ttccccgatc atcctgttaa aaagtgttga
     1921 ttttcctacg tttggttttc ctacaatcag aactatcgga ccggacaaaa cttttcacct
     1981 caaataattt agttttcttg gttgacctga ttcagaacct cctgtgtttc tttgttttct
     2041 ctgtctctta aaaggtcaac tatacttata ttcatatttc cttttctttc ctcagtttca
     2101 aaatcaattt taagaacctt ggcttcaact tcatcacccg ggttaagaat agtctgtatg
     2161 ctttcaacct tttcaacgct tgcattctta acagaaagaa atgcttcaac atcgtacttg
     2221 tctataagca ctacagcacc tgaaccctgg tatcttaaaa ccttaccctt aaccgtatcg
     2281 cctttcttta cttcctggga aaccagcttc caaggattat ctatagcctg ttttatcgaa
     2341 agttttattt tcctgttttc cttgtctata ttaaggactt ttacttttac tttttcactt
     2401 tccttcaaag cttcctgagg attgtccaca aagttccatg aaagctctga cacatgtgca
     2461 aaacccgata taccctcttc aagcttaatt atagcaccgt ttggcagaag tttttcaacg
     2521 taaccggtgt atatttcatt ttcagagtac ttatcgtcaa tatctgtcca tgggtcgccg
     2581 atcgcctgtt tgtaacttaa ggtaagcttc ttgttctgag tatctactcc cagaactttc
     2641 acttcaattc tgtccccaac ttttgcaaca tcggagattc ttcccttttt tacccagaat
     2701 atttcagact cgtgaataag gccttcaact cctggctcaa gctttactgt aaagccaaaa
     2761 ggaagaatct gagttacaac tgctcttaca atacagtcta ccggatattt ttcttcaatt
     2821 tttatccacg gatcttcagt aagctgtttg agcgaaagag atatttttct gttttcctta
     2881 tcgactttaa ttatcattcc ccttactttt gaaccaactt tcagaaccgt ttccagaggc
     2941 atatttctgt cccatgtagc ttcacttttg ggaataagag ctgtaacact atcgtttatc
     3001 ctgacaaatg cgccaaactc ttttatgctc tcaacaatgc cttcaactac catattctct
     3061 ctaagatcct catatgcctt gtttagcttt tcgtcaataa ggtcttttct agaaactgtt
     3121 atacttctgc cccttattcc gattacttta acttccacaa gacctttggg catagcatcg
     3181 ccctgtctca gaccggaaag tgaaccagga aggaatgatt tgaggactcc gtttacaaaa
     3241 acatcgtatc ccttaagatt cttagcctta aagattactt ttacaacgtc tccttccttg
     3301 atttcgtttc tgagcttatc aaacattccc tgtttttcag aagcgtggtt taaaccctct
     3361 tcatcgttag tttttactat ttttaccgtt acttcctcac cggatttata ttcagaaata
     3421 tctcttgtaa ggtcttccct ctttacaaaa acatcaccgt tggaaccctc caacgctacc
     3481 catataccgt ctgtatcgct ttcaaaaacc tttcctgtaa aaacttttcc ttttttgagc
     3541 tcgttaaact cgttgtcgtt gagcatctgt tcaaaatcgc tcatgttttt tctatcttcc
     3601 atgttagata gacctcccct ttatcttaag aaaaattacc acaatcaacc atgtggactg
     3661 catctattat tttctctatt tcccacaaag gagcagaagt tccgctgagt actcctattg
     3721 tttttacttg tgaaaaatca agttttttaa gatcctctga cgtctgtatc agataactcc
     3781 tgctgcagcc gcggcatgcg atatcataaa gctttctggt gttagagctg ttactgctgc
     3841 ctataatcaa cattaaatca gcctttaatg ctatttccgc cgcttgtttc tctctttgca
     3901 ctgtgtgaaa acatatagta ttcttaacta ttatatctga aaaatcattt tttttgatga
     3961 tatcggataa aaattcttta aaatcctgcg tatccattgt cgtctgggat aaaatacata
     4021 cacgggactg ctgaactatt accgctctgt cagtaataac tgccttttca gggtcaatac
     4081 ttcccaaaag ccctatcatt tcggcgtggt ttttcttacc gaaagcaatt atatagtagc
     4141 cgctgtcctg catctttttt gcaaactcta ccattgcaga tactatagga catgtcatat
     4201 ccaacacatg agcaaaactg ctgtgtagaa cagacatatc atgcgatcct atgccatgag
     4261 ccctcactat tacacggctc tgccgggaat caaccggaag gttgtttata tcttcaaagt
     4321 aagatatacc ctctttctga agctttccga caacctggtc gttatgcaca agcaacccat
     4381 aaatgtaagt ctttttttct gatgtatcca gcataagaca ttccttgtac gctctgttta
     4441 ccccgtaaca aaatcctatt tcatcggata cgataacctt catacactca acctcttata
     4501 tctccagaat ctacgtaccc ggcaattata tcgcacaccc gctctatctc catgccagtc
     4561 gtatctatta tcacagcatc gggggcaggt ttaagcggag ctatgctcct gctcgaatct
     4621 gccctgtccc tctgatttat ctcatttagt acatcttcga acgaaacatc ctcatttttt
     4681 gcaattaact ccctgtatcg tctctcagcc ctttcctgtg gacttgccgt gagaaaaaat
     4741 ttgtaccgtg catcttttag cacaaccgta ccgatatccc ttccgtcaac aaccatactg
     4801 catttttcgg atatactgcg tatcacggag ttcacttttt ctcttacctg acctattcgt
     4861 gagactgcag aagcacttgc tccagactgc ggttttctta tctgggctga tattacctca
     4921 ccgtttaata tgtaatgtcc atccgcataa actatgctta tttcgtccag atgactctga
     4981 atataaagaa cgtcggcaag atcaacacca ttaccctgaa aataataggc aatcatacgg
     5041 tacaaagccc cgctgtcaag ataaagtatt cccagtctct ttgatatttc tttggccacg
     5101 gtagactttc ccgatccggc aggaccgtct attgctatat gaaacaattt tttcattctc
     5161 cttatttata ataccggtat ttatatttac ggttctgcac tattttttta tctcttcttc
     5221 ggtatctact attcttatta tcttgtcaag cttggtcatt tcaaagatct tcttgacctg
     5281 tggctgcaac gaaaagagaa ccagctcctt ttccgtcttt tttgattcct ttataagcga
     5341 tacaatcgaa cccaatcccg cgctgtctat gtacggaacc tccgaaagat caaaggccac
     5401 tatcttcttg tcggtatgag gcatatgctc aagggttttc tgtttgaaag tggcagaatg
     5461 ataggcatcg atgtctccgc atacctttat tatgaaagcc acatcagaat ctttaaaact
     5521 aatgacaaat tccatggtca caccctcctc ttttatggat taagagaaat tttaagatct
     5581 ctcagctgtc tttctcctac tatgtttggc gcatccgcca ttatatccgt tcccgtagcc
     5641 gtcttggg
//
LOCUS       DAOSHR010000136         5624 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_12736_length_5624_cov_4.098402, whole genome shotgun sequence.
ACCESSION   DAOSHR010000136 DAOSHR010000000
VERSION     DAOSHR010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 5624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5624
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12736_length_5624_cov_4.098402"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(316..1335)
                     /locus_tag="PLU28_07875"
     CDS             complement(316..1335)
                     /locus_tag="PLU28_07875"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HOG35017.1"
                     /translation="MKRIAVLNVGGDCPGLNAVLRALIVKGAEEDIEIIGVYDGFKGL
                     VEDRMFIMTKEHVSGKLPEGGIILGSSKFDPYNNSEDFKKLKRNVQDYQITALVLLTG
                     HTGAKIALRLKEDGISSIIIPATIDNDLNWTHMSIGFLTGLQTVTTALDYLHSTASSG
                     HRVIVVEVGGDEAGWLAAIGGMAGGADYIMVPEVEVNPQEMLANINRRYKFGKRFSII
                     VVEEKCKLPQEITKVADGIKAPHLITVCDVVAEYIRENMPKDFDCQTLNLGYMQRGGS
                     PLAFDRFLAFKFGISAIEYVKRGEVNVALGLNGFDISHMPYSEDILINREIRPGLYEM
                     AKLFF"
     gene            complement(1353..>3010)
                     /locus_tag="PLU28_07880"
     CDS             complement(1353..>3010)
                     /locus_tag="PLU28_07880"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HOG35018.1"
                     /translation="AVSSLGKALRVAFNGGAVMGMTVASLGIFGLGTVFFLSNGNSVL
                     LSGYAMGASFVALFARVGGGIFTKAADVGADLVGKTEAGIPEDDPRNPAVIADNVGDN
                     VGDVAGMGADLYESYVGSIFSASVLGTVAFSALGGVFPFTVAASGILSSIIGIVFVNL
                     TIKESEKINPSFMLNLGTYIANAIEVVFVLILSKLLLKSYTAGMIITLGMIVGILIGA
                     LTEYYTAKNPVKKLAKSATSGAAPLLINGLAVGMESTALSVILISIVTIISFRFLGLY
                     GIALGGIGMLATLGITLAIDAYGPIADNAGGIAEMSHLDPSVRSRTDKLDAVGNTTAA
                     IGKGFAIGSAALTALALFASYTQVTGINIVDIKESTVFIGVLIGAMLPFLFSAMTMKA
                     VGDSAQEMVAEVRRQFKEIPGIMDGKSDPDYARCVSIATGGALKKMILPSLLAVLSPI
                     IVYFILDKVAVAGLLVGTTVTGVLLAIFMSNSGGAWDNAKKHIEEGNYGGKGSMAHKA
                     AVIGDTVGDPFKDTSGPAINILIKLMSIVSIVIIPVIIKLIEG"
     assembly_gap    3011..3110
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3111..3493)
                     /locus_tag="PLU28_07885"
     CDS             complement(<3111..3493)
                     /locus_tag="PLU28_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HOG35019.1"
                     /translation="MDEFLRTIGISSGIIGLLFTFFLSKRVLKNDPGNEKMVKISNSI
                     QIGARAFLFSEYKIMYVLLIIFSLLLGFIQSWQMGLTFIFGATLSVLSGFIGMSIATK
                     ANCRTAQAAVSSLGKALRVAFNGGAV"
     gene            3764..4024
                     /locus_tag="PLU28_07890"
     CDS             3764..4024
                     /locus_tag="PLU28_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35020.1"
                     /translation="MQKFLAVTLSLFLTTPGFSVKSLLAGKNLPVLKKSNQSYLCAPK
                     YGTQNRISQKTADRIADAACIVDTRSIVDTRSIVDTRSIADT"
     gene            4149..4823
                     /locus_tag="PLU28_07895"
     CDS             4149..4823
                     /locus_tag="PLU28_07895"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOG35021.1"
                     /translation="MANNNAKAKKYVVVIDPGHGGSDPGTLGQLGSKEKDINLLVAEK
                     VKQLLSKYKVTVQLTRTDDSYVEVHERALMSNFYEADLFISIHLNSYTENREIRGSEI
                     YYFDYQREAYTKRLILKENFDEKKDKKVIEEWVNDKENSIKPSIKFANIIADNVEKNG
                     IKLRGVMPNLDLAVMAYGRAPTVLFELEFLSNLTVEAKFKNGIYVDLFAKIIKDSILQ
                     YFDIRQ"
     gene            4953..5029
                     /locus_tag="PLU28_07900"
     tRNA            4953..5029
                     /locus_tag="PLU28_07900"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4987..4989,aa:Pro,seq:tgg)
     gene            5042..5116
                     /locus_tag="PLU28_07905"
     tRNA            5042..5116
                     /locus_tag="PLU28_07905"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5074..5076,aa:Gly,seq:tcc)
     gene            5117..5193
                     /locus_tag="PLU28_07910"
     tRNA            5117..5193
                     /locus_tag="PLU28_07910"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5151..5153,aa:Arg,seq:tct)
     gene            5241..5315
                     /locus_tag="PLU28_07915"
     tRNA            5241..5315
                     /locus_tag="PLU28_07915"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5273..5275,aa:His,seq:gtg)
     gene            complement(5350..>5624)
                     /locus_tag="PLU28_07920"
     CDS             complement(5350..>5624)
                     /locus_tag="PLU28_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35022.1"
                     /translation="VTSTIIFSALPKERVAIINSLSNFMNNIVRGVASGLFGIIVSSG
                     LSGYKTLFLISAVFYILNIIMYLWTYFPIRNSTLVTELYKKENVHP"
BASE COUNT         1717 a         1130 c         1075 g         1602 t
ORIGIN      
        1 ttttcaatcc aaagccggca ccattggcaa aaggtgttct ctgggcacca tgggcgatat
       61 ggaaagactg acagataact gattgagtac aataagtgca ggtccttaag gggcctgcac
      121 tttttttatg tacgcataca tgactttggt aatgtttttt cattactttg ttcactaaaa
      181 aaaagatcat aaagaatata taatgaaagt acaacaaaat acttcgctct ccccagagtt
      241 ttgagttatt ttgctaatgg tctctgacca taaaaaacat cccgtaaaag ggatgttttt
      301 aatttttgta tttaatcaga aaaacaattt tgccatttcg tacagacccg gacggatttc
      361 acggttaata aggatatctt cgctgtaagg catatgtgaa atatcgaagc cgttgaggcc
      421 cagtgcaaca ttgacttcac cgcgttttac atactctatt gcgcttatac cgaatttaaa
      481 tgccaagaat ctgtcaaaag ccagcggaga tccccctctt tgcatatatc caaggttaag
      541 tgtctggcaa tcgaaatctt tgggcatatt ttcccttata tactcggcaa ctacgtcaca
      601 taccgtaatt aaatgaggag ccttgattcc atcggcaact ttggttattt cctgtggaag
      661 tttgcatttt tcttctacta caattattga aaatcttttt ccgaacttat atcttctgtt
      721 gatattggca agcatctcct gaggatttac ttctacctcc ggaaccatta tataatcagc
      781 tccgccggcc attccgccta tagctgccag ccatcctgct tcatctccgc ctacctcaac
      841 aacgattacc ctgtgacctg agcttgccgt agaatgcaga tagtccaaag cagttgtaac
      901 tgtctgaagt ccagtcaaaa agccgatact catatgtgtc caattaagat cattatcaat
      961 agttgccggg atgattatgc ttgatatccc gtcttcttta agtctaaggg caatttttgc
     1021 tccagtatgt cctgtgagca gaacaagagc cgttatctga tagtcctgaa catttctttt
     1081 gagtttttta aagtcctcgc tgttgttata aggatcaaat tttgaagaac caagaattat
     1141 accgccttca ggaagttttc cggaaacatg ttctttggtc attataaaca ttctgtcttc
     1201 aacaagccct ttaaaaccgt cataaacacc tattatttct atatcttcct ctgccccttt
     1261 tacgataagg gcccttaaaa ccgcgttaag tcccggacag tcaccgccta cgttcaaaac
     1321 agctattctt ttcatatttt ctacctcccg gattatcctt ctattagttt tattataaca
     1381 ggaataatga ctatagaaac tattgacatt aattttataa gaatattaat tgccggtcct
     1441 gatgtatctt taaaaggatc gccgactgta tctcctatta cagctgcttt atgtgccatg
     1501 gaaccttttc cgccgtaatt accttcttca atatgcttct tagcgttatc ccaagcacct
     1561 ccggagttcg acataaatat agcaagaagc acgcctgtga ctgttgttcc tacaaggagt
     1621 cctgcaacgg ctactttgtc cagaataaag tagactatta tgggtgaaag aacagctaaa
     1681 agagatggaa gtatcatttt tttaagggca ccgccggtcg ctattgagac acatctggca
     1741 taatcaggat cactttttcc atccataatt ccgggtattt ccttgaactg tcttcttact
     1801 tcggcaacca tctcttgtgc agagtcgcca acggccttca tagtcatagc tgagaaaaga
     1861 aaaggaagca ttgcgcctat aagcacgcct atgaatactg ttgactcttt aatatcaaca
     1921 atatttatac cggtaacctg tgtataagat gcgaaaagtg caagagcagt cagcgcggca
     1981 gaacctattg caaacccttt tcctattgca gcagttgtgt tgcctacagc atcaagctta
     2041 tctgttcgtg aacgtacgga tggatcaaga tgggacattt cagctattcc gcctgcatta
     2101 tcagctatcg gaccgtaagc atctatggca agggttattc ccagagtggc aagcatgccg
     2161 attcctccca gagcaattcc atatagcccc agaaatctga aagatattat agtaactata
     2221 gatataagaa ttaccgaaag agctgtagac tccattccca cagccaagcc gtttatcagc
     2281 agaggagccg ctccggaggt agcagatttt gcaagtttct taaccggatt tttagcagta
     2341 tagtactctg taagcgctcc gataagtatt ccgacaatca tccccaaagt gattatcatg
     2401 cctgcagtat aacttttgag aagcagtttt gaaaggataa gtacaaaaac tacttctatg
     2461 gcgttggcaa tataagtacc caggttaagc atgaaagaag gatttatctt ttcagactct
     2521 tttatcgtga gattgacaaa aactattcct attatggacg aaagtatgcc gcttgccgca
     2581 accgtaaatg gaaaaactcc tcctaaagca gaaaaagcta cagttccaag aacggaagct
     2641 gaaaatattg aaccgacata agattcataa agatcggctc ccatacctgc tacatctccg
     2701 acattatctc cgacattatc cgcaattacg gcaggatttc tagggtcatc ctccggaatg
     2761 ccggcctcag tttttccaac cagatcggct cctacatcgg cagcctttgt gaagataccg
     2821 cctccaacac gtgcaaaaag tgccacaaag gaagcaccca tagcatatcc gcttaataac
     2881 acggagtttc cgtttgaaag gaagaataca gttcctaatc caaagattcc aagggatgca
     2941 accgtcatac ccatgacagc tcctccatta aaagctactc tcaaggcttt tccaagagaa
     3001 gacacagccg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn acagctcctc
     3121 cattaaaagc tactctcaag gcttttccaa gagaagacac agccgcctga gccgttctgc
     3181 agtttgcttt tgtggctatg ctcattccta tgaatcctga gagtacagaa agagtggcgc
     3241 caaaaatgaa ggtcagtccc atttgccagc tctgtataaa tccaaggaga agtgaaaaga
     3301 ttatcagaag aacatacatt atcttatatt ctgaaaacag aaaagctcta gctcctatct
     3361 gaattgagtt agagattttt accatttttt cgtttccggg atcatttttc agtacccgct
     3421 ttgaaagaaa aaaagtaaaa agtaatccta tgattcccga agaaattcct atcgttctga
     3481 gaaactcgtc cataccaatc cctcccgaag aacatgtttt ataaagatat tttatcatat
     3541 aaaaagattt ctgtaaacat tttaaaaaga ttaattacaa taaatactga atcatatctc
     3601 acttaaacag gttcaaacta ctaattaccc caaaaaacaa ggtattctgg gcatacattt
     3661 gtttaaaatc aaaaattatg ataaaatgag taaaagagtt tataagaatt ttttaagttc
     3721 tttgaggtag gattttataa attcaaaaaa gggggaacag aaaatgcaga aatttttagc
     3781 ggtgactctg agccttttcc tcacaacacc gggtttctcc gtaaaatccc ttctcgccgg
     3841 taagaacctt cctgttctaa aaaaaagcaa tcaaagttat ctgtgtgctc caaagtacgg
     3901 tactcagaat cgcatctctc aaaaaacagc cgataggata gccgatgcag cttgtatcgt
     3961 agatacaaga agtatcgtag atacaagaag tatcgtagat acaagaagta tcgccgatac
     4021 ctagagtatc gccgataata tctaatagta tggatggtga atagttgcac tcctataaaa
     4081 atagaatttt tttgaaaagt ctttttatat tttgtgtttt atccttttgt actctaacgg
     4141 tttatggaat ggcaaacaat aatgcaaaag caaaaaaata tgtggtggtc atagatccgg
     4201 gacatggagg tagtgatccg ggaactttgg gtcagttagg ttcaaaggaa aaagatataa
     4261 atcttttggt ggctgaaaaa gttaaacaac tgctttccaa gtataaagtt actgttcagc
     4321 tgacacgtac agatgacagc tatgttgaag tgcatgaaag agctttgatg tcaaactttt
     4381 atgaagcaga tctttttata agtatccatc ttaattcgta taccgagaac agggaaataa
     4441 gaggaagtga gatctattat ttcgattatc agcgtgaggc ttacactaaa aggctaattc
     4501 tgaaagagaa ctttgatgag aaaaaagaca aaaaagtcat tgaggaatgg gttaatgata
     4561 aggaaaattc aataaaaccc agcataaaat ttgctaatat aattgcagat aatgttgaaa
     4621 agaacgggat taagctacgt ggggttatgc ctaaccttga tctggcagtc atggcctacg
     4681 gacgagcacc tacggtttta ttcgaacttg agtttttaag taaccttact gtggaggcta
     4741 agtttaaaaa cggcatatat gtagaccttt ttgcaaaaat aattaaggat tcaattctgc
     4801 aatattttga cataaggcag tagacgtaaa aagaaaaact cagtaagcag gtaaaaacct
     4861 ttacatgtaa catacactaa actattttga aaaaacttaa tattgacaaa ccctgtcagt
     4921 tgtgataata tatatttcgt aagagttgag tacggagcgt agcgcaggtt gggagcgcgc
     4981 cggtcttggg caccggagga cgctggttca aacccagtcg ctccgaccat attttccaag
     5041 tgcggctgta gttcaacggt agaataacgg ccttccaagc cgtgggttgc gggttcgaat
     5101 cccgtcggcc gctccagcgc ccgtagctca actggataga gcactggatt tctaatccgg
     5161 gggttgggag ttcgagcctc tccgggcgcg ccagtgtaaa gtataggcgg cttatagccg
     5221 tctgtatttt atgaggcatg gtggttatag ttcagcggta gaacgcctga ctgtggatca
     5281 ggatgtcgtg ggttcaaatc ccactagcca ccccataaaa aaaacaacaa ctaaaagttg
     5341 ttgttttttt tatggatgaa cattttcttt tttatatagt tccgtaacca aagtactgtt
     5401 tctgattggg aagtatgtcc aaaggtacat aatgatattt agaatataaa aaacagcgct
     5461 tatgaggaaa agagtcttat aaccagacag acctgaagat acgatgatgc caaacagacc
     5521 ggaagccaca ccacgtacaa tattgttcat gaagttgctt aaactgttta ttattgcaac
     5581 tctttcttta ggtagggctg agaatatgat ggtgcttgtt actg
//
LOCUS       DAOSHR010000137         5616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_12765_length_5616_cov_4.029491, whole genome shotgun sequence.
ACCESSION   DAOSHR010000137 DAOSHR010000000
VERSION     DAOSHR010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 5616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5616
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12765_length_5616_cov_4.029491"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..876
                     /locus_tag="PLU28_07925"
     CDS             <1..876
                     /locus_tag="PLU28_07925"
                     /inference="COORDINATES: protein motif:HMM:NF014026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HOG35023.1"
                     /translation="AFQNNHEYDILFEKGCEINSEDPASSVRAESIASQSDLVIAVMG
                     ESNNMSGENNNRSSIELPKPQRELLKKIYTINKNIILVVMSGRPIALEWEDEHMAAVL
                     QAWHLGDESGNAINEILTGLAVPSAKLTATVPRSTGQIPMYYNHKNTGRPSFRHYIDV
                     ADSELYPFGFGMSYTTFEYSNLRTDKKVLHEGESVTLKVDIKNTGKLQAYEICQIYMH
                     DPVSEFSRPVKQLVAFKKISILPGETQTLSFTFSTDIFAVLNSQYLPTVEKGTIELFA
                     GTNSRDLIKTTIEIL"
     gene            895..2001
                     /locus_tag="PLU28_07930"
     CDS             895..2001
                     /locus_tag="PLU28_07930"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOG35024.1"
                     /translation="MNIFVVGYEHPKTDKRVFRTVSSLAQKAENKIIYQYWTDNINEK
                     RQTQGSITYCPVFYDKNSSVSALYKLKRRKAFDNIIMDMILHEDYDVIIFNYFVISYL
                     TKAFSAAKKRNKKIIYDIHELHPENFLNLMTGFKAKLKQAILWKALKKQLKLSDKVVY
                     VSQEIKEEIIYKTKVEKAFLIVPNYASVKLDPDPKKKIKDILYVGGVKRSLEQERKLI
                     SFFQNEGYTFTILGMKYKMDMEKILYEEYKPYGEMMHRINQSRFSLVAYQTSGRKDYK
                     NDLYALPHKFYDSVAAGTPVIIKKEFVSMAKLVEKYQIGVVADTSDIQKTALAIHQAE
                     ENYPTLMENIRKFQDEFVWTDAKQKEYIDFITQL"
     gene            complement(2328..3626)
                     /locus_tag="PLU28_07935"
     CDS             complement(2328..3626)
                     /locus_tag="PLU28_07935"
                     /inference="COORDINATES: protein motif:HMM:NF014686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOG35025.1"
                     /translation="MKNVLVYPCGTEIALEFYRAMAYSTHYSIIGGVDSYDHGRFVFE
                     NVIEGLPFITDKSTKEDIMEFQHKIEPYDIDLIYPAMDGVIKKFSDYREYLKPILCIC
                     NKETNELTRSKGKTYLALEKYIHVPKTYENIESVKEEDFPVFVKPDVGQGAVGAKKIN
                     SKKELVNFFDGNDKKYVICEFLPGEEYTVDCLTNKSGKLIFSQARARKRIKDGISVNS
                     ISIDDTQLTTIAQTINSHVSFKGAWFFQVRKNNQGEYVLMEIASRIAGASSYTRAMGV
                     NLPELTLEIFSGSDIDFVLPNDYTVEQDRALYNAYDSNISFSKVYMDYDDTVTCRGKL
                     NDQIIQFIAKCKNHKIPVILLSRHDGDLNTELSNWGITGLFDKVVHMDRKKPKSDFIS
                     DKNSIFIDDSFGERKQVKEAVGIPTFEPSMVEFLINRRGF"
     gene            complement(4140..5018)
                     /gene="rfbA"
                     /locus_tag="PLU28_07940"
     CDS             complement(4140..5018)
                     /gene="rfbA"
                     /locus_tag="PLU28_07940"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016283109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HOG35026.1"
                     /translation="MKGIILAGGSGSRLYPITKSISKQLVPIYDKPMIYYPLSVLMLS
                     EIQEVLVISNPEYIELYRHLLGNGSSIGMSIEYKVQEQPRGLADAFIVGESFINGESV
                     CLILGDNIFYGQDFVPRLKRASKITEGAMIFGYYVNNPRDFGVVEFDKDNNVVSLEEK
                     PEKPKSNYAIPGLYYYDSTVVQKAKGLEPSKRGELEITDLNREYLKEGKLRVELLGRG
                     FAWLDTGTYDGLADASDFVRTMQKRTGLYVSCIEEIAYRNRWISKEQIISIGKEHEKT
                     EYGRYLLSIAKDIRVE"
     gene            complement(5015..>5616)
                     /locus_tag="PLU28_07945"
     CDS             complement(5015..>5616)
                     /locus_tag="PLU28_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOG35027.1"
                     /translation="MFSFHATKVFNTIEGGAVTFRDEKLIKILNDLKNFGITGPETVE
                     YAGGNAKMNEFQAAMGICNLRHVEEEINKRRKVYERYLSNLEGIKNIKIVRPAEGIKS
                     NYAYFPVIFDGYRCNRDEVFELLKENGIYARKYFYPITSTFECYTGVFAPQKTPAALE
                     ISEKVLTLPMYADLKMEEVDRICSVILKSSRYFQAGGEK"
BASE COUNT         1746 a         1156 c          886 g         1828 t
ORIGIN      
        1 gctttccaaa acaatcatga gtatgatata ctttttgaaa agggctgtga aataaactct
       61 gaagatcctg cttcttctgt cagagctgaa agcatcgctt cccaaagcga ccttgtgata
      121 gcggtaatgg gtgaaagcaa caatatgagc ggagagaaca acaacagatc ctccatagaa
      181 ctgcccaaac cacaaagaga gcttttgaaa aagatctaca ccattaacaa gaacattatc
      241 cttgttgtaa tgagcggacg tcccattgct cttgaatggg aagatgaaca tatggcggct
      301 gtgcttcagg catggcatct tggggatgaa tcaggaaatg caatcaatga aatactgaca
      361 ggtctggctg taccaagtgc aaagcttacg gccacagttc caagatcaac gggacagatt
      421 cctatgtact ataatcataa aaataccgga agaccgtctt tcagacatta tatagacgtt
      481 gccgattccg agctctatcc tttcggattc gggatgagtt acaccacttt cgagtactca
      541 aacctcagga cagataaaaa agtattacat gagggagaat cggtcactct gaaggttgac
      601 ataaaaaata ccggaaagct ccaggcttac gaaatatgcc aaatatacat gcacgaccct
      661 gtatccgaat tttcaagacc ggtaaaacag ctcgtggcgt ttaaaaaaat ctctatcctc
      721 cccggagaaa cccaaacact ttcttttacc ttttcaacag atatttttgc ggttttaaac
      781 tcacagtact tacccacagt tgagaaaggt acgatagagc tgtttgccgg aacaaacagc
      841 agagatctta tcaaaacaac gatagaaata ctctgaaagg agaaaattca aaatatgaac
      901 atttttgttg tcggatacga acatcccaaa accgacaaaa gagtcttcag aactgtcagc
      961 agtcttgctc aaaaagctga aaacaaaata atttaccagt actggaccga taatataaac
     1021 gaaaaaagac agacccaagg ctctataact tactgccctg ttttttatga taaaaacagt
     1081 agtgtgagtg ccctgtataa gctaaaaaga aggaaagcgt tcgataacat cataatggac
     1141 atgatactcc atgaggatta cgacgttatt atcttcaatt actttgtgat ttcatacctt
     1201 acaaaggctt tttctgcagc aaaaaaaaga aataaaaaga taatctacga tatccatgag
     1261 cttcatccgg aaaatttttt aaaccttatg acaggtttca aggcaaaatt aaaacaggct
     1321 attctctgga aagccttaaa aaaacagctg aaattaagcg ataaagttgt ttatgtttcg
     1381 caggaaataa aagaagaaat tatatataaa acaaaagttg agaaagcttt tcttatcgtt
     1441 ccgaactatg cttcggttaa acttgacccg gatccaaaga aaaaaattaa ggatatctta
     1501 tacgtcggag gggtaaaaag aagtctggaa caggaaagaa agctcatttc tttctttcag
     1561 aatgaaggat atacttttac catacttgga atgaagtata aaatggatat ggaaaaaatt
     1621 ttatacgagg agtataagcc atacggcgaa atgatgcaca gaataaacca aagccgcttt
     1681 tcgcttgttg catatcagac ttccggccgc aaagactata aaaatgactt gtacgccctt
     1741 ccgcacaagt tctatgactc tgttgcagca ggaacaccgg ttataataaa aaaagagttt
     1801 gtttcaatgg caaagcttgt agaaaagtat caaatcggag tagttgcaga tacctcagat
     1861 atccaaaaga cagcactggc catacatcag gccgaggaga actatcccac acttatggaa
     1921 aacatccgca agtttcagga tgagtttgta tggaccgatg ccaaacagaa agagtatatt
     1981 gatttcatca ctcaactctg atatcttttg cgatgcttat cggaactcag tgtttggtac
     2041 tgacttgtac ggtcactatg cagtatgcaa ttattaattt ttacgagccg tttgaccgta
     2101 tctatacata gctccttaaa ctcccgtata aacagatgtc gttttgatgt ttttacttct
     2161 tccagtcttt tacgaaaaaa ccaagtgcat cctttaagat atcgttagct tttaaacacc
     2221 tcttctatag attttattct atcaattttt ggggtgtttg tcgactctac ttttatggta
     2281 actctccata aattaatttc catttcttaa taattaagtt ctcctattta aaatcctctt
     2341 ctgtttatta agaattccac catcgatggc tcaaacgtag gtatgcccac tgcctctttt
     2401 acctgcttac gctctccaaa cgaatcatca ataaaaatcg aattcttatc agaaataaaa
     2461 tcacttttgg gcttttttct atccatatgc acaactttat caaagagccc ggttatcccc
     2521 cagtttgaaa gttctgtatt aagatcgcca tcgtggcggc ttaatagtat aaccggtatc
     2581 ttatgatttt tgcatttcgc aataaattgt ataatttgat cattcaactt tccacggcat
     2641 gtaactgtgt catcataatc catatatact tttgaaaaac taatatttga atcatacgca
     2701 ttatataatg ccctgtcctg ttcaacggta taatcattag gcaacacaaa atctatatca
     2761 gaaccagaga aaatttctaa tgtcagctct ggaagattta ctcccattgc ccgagtatat
     2821 gacgaagcac ctgcaattct tgaagcaatc tccatcaata catattcccc ttgattattc
     2881 tttcttacct gaaagaacca ggcacctttg aacgaaacat gactattaat tgtttgagct
     2941 attgtggtaa gttgcgtgtc gtctatactt attgagttca ctgaaattcc atcttttatt
     3001 ctctttcttg ctcgggcctg tgaaaatatt aatttgccag atttattagt aagacaatct
     3061 accgtatact cctctccagg aagaaattca catatcacat attttttatc atttccatca
     3121 aagaaattaa caagttcctt tttggaattt atctttttag caccaacagc gccttgtcca
     3181 acatcaggtt taacaaaaac tggaaaatct tcttctttta cgctttctat gttctcatat
     3241 gtttttggca catgaatata cttttctaat gccaaatagg ttttaccttt tgatcttgtt
     3301 aactcatttg tttctttatt acatatgcat agaataggtt tcaaatattc tctatagtca
     3361 gaaaacttct taattacacc atccattgca ggatatatta aatctatatc atagggctca
     3421 attttgtgct gaaactccat gatatcttcc ttcgtacttt tatcagtaat aaacggaagt
     3481 ccctcaataa cattttcaaa tacaaacctg ccatggtcat atgaatcaac acctccaata
     3541 attgaataat gtgttgaata tgccattgct cgataaaatt caagtgcaat ttcagttcca
     3601 catggataca ctaatacatt tttcatgata tttcctccga attctcaaca aatatactta
     3661 ccttgctaat ataaatactt tcatatacta ataattacga tccgtttcgc atgaagtcat
     3721 ctctttcttt tttccgggca tagttattat ttatgcttat tttgccccac aaccaaaaat
     3781 attgtatgga tttaaacagt cctgttacat caataattta tactcatctt ctttgatttc
     3841 ctagctactt ttctgtatct tcactttcat cttcctctgg aatcatatag caaaaacctt
     3901 caaagatacg atttcaacca ttgatttttc ataaaactat agatttttta tttccgtgct
     3961 taatccttaa aaataattag aaaacatttt atatatgtaa attatagcat gaatacagta
     4021 aaatataata actagtttgt agatacaaat ctttgttgcg ttttattctt ttttatactt
     4081 tacgataaaa cagatattaa ttaataactc ggaaaactaa aaagaatata ttgatttcat
     4141 cactcaactc tgatatcttt tgcgatgctt aaaagatatc tgccatactc tgtcttttcg
     4201 tgttctttac cgatcgaaat tatttgttct ttgcttatcc acctgtttct ataagctatc
     4261 tcttctatgc aggatacata aagacctgtt cttttctgca tagttcttac aaaatccgat
     4321 gcatccgcaa gtccgtcata tgttcctgta tccagccatg caaagcctct tcccagaagc
     4381 tccactctta gcttcccttc tttaagatac tctctgttca ggtcagttat ttcaagctct
     4441 ccacgtttgg atggctcaag acctttggct ttctgcacaa ccgtactgtc ataataataa
     4501 agtccgggaa tggcatagtt ggacttaggt ttctctggtt tttcttcaag cgatactaca
     4561 ttattatctt tatcaaactc cactactccg aagtctcttg ggttatttac atagtatccg
     4621 aatatcatgg ctccttcggt tatcttggaa gctcttttta accttggaac aaaatcctgt
     4681 ccgtaaaata tattatctcc tagaataaga cagacgcttt ctccgtttat aaaactttcg
     4741 cctactatga aggcatccgc aagtcctctg ggttgttcct gcactttata ctctatgctc
     4801 atccctatac tgcttccgtt gcccaaaaga tgtctatata actctatata ctccggattg
     4861 gaaatgacca gcacttcctg gatttctgaa agcatcagaa ctgacaaagg atagtatatc
     4921 atgggtttgt cgtatatcgg aacaagctgt ttgcttatcg atttggttat tggatataat
     4981 ctgcttccgc ttccgcctgc aagtattatc cctttcattt ctcacctccg gcctgaaagt
     5041 atctgctgct tttaagtatt accgaacaga tcctatctac ttcttccatc tttaagtctg
     5101 catacatcgg aagagtcagt accttttcgg atatttccag tgcagcaggc gttttttgag
     5161 gtgcaaacac tcctgtatag cattcaaatg tacttgtaat aggatagaag tatttacgcg
     5221 catatatgcc gttttcttta agaagttcaa acacctcatc cctgttgcac ctgtatccat
     5281 caaaaataac aggaaagtac gcatagttac ttttgattcc ttcagcaggc cttactattt
     5341 ttatattttt aattccttca agattactaa gatatctttc gtagacctta cggcgtttgt
     5401 taatttcttc ctctacatgc ctaagattgc atatccccat agccgcctga aactcgttca
     5461 tcttggcatt tcctcctgca tactctactg tttccggacc ggttatgcca aaatttttaa
     5521 gatcattcag gatttttata agtttttcat ctctaaatgt aacagctccg ccttctatcg
     5581 tattaaagac ttttgtggca tggaaactga acatag
//
LOCUS       DAOSHR010000138         5497 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_13121_length_5497_cov_5.632672, whole genome shotgun sequence.
ACCESSION   DAOSHR010000138 DAOSHR010000000
VERSION     DAOSHR010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 5497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5497
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13121_length_5497_cov_5.632672"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..919
                     /locus_tag="PLU28_07950"
     CDS             <1..919
                     /locus_tag="PLU28_07950"
                     /inference="COORDINATES: protein motif:HMM:NF013601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HOG35028.1"
                     /translation="DASEKERKEYFENEYANEANFLPVKDNLPKYSAILIDEVQDYDS
                     RWIAILRKFFLKENGELVLFGDEKQNLYGRAVDGAEKKPNTGIPGRWNKLKKLKRFKG
                     IISPMIINFQKQFLSGKYLVDEVESFQTSILDFKLEYLWVDEQSTVYGELLHEIGKAE
                     NNGENVVVLGSSIEYLRVFEKFYRQKTQKATKSSFETQQEYEEIKAKLDKEKKSNMIK
                     TLCEEIRKDRKLNFWSESRNGHELKISTIQSFKGMEMNNVILLIGENDQEELVYTGIT
                     RAREKLVILNNGNGKYHDFFAKYSILKAA"
     gene            936..1751
                     /locus_tag="PLU28_07955"
     CDS             936..1751
                     /locus_tag="PLU28_07955"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOG35029.1"
                     /translation="MEGYFLATGGGSEIGASSYCLSLGNNRVILDCGMRFGERKYPSI
                     DSLFKEGIFPGSSQTGSIIVSHSHKDHFGALPYFYQNGFQRIISTPQCKDNLIKRVSA
                     CLDTFNIDQYLNLKQVFMSIAAYDFFEPFSCGDLDCIFVPAGHIDGAAMTVIFYEGQK
                     LVYTGDFSFPLSSDEVENSCAFEFIKDADVLITESTYGYMKNKTVISSKHAQFEDLCH
                     LIEKIYPKTIFLVHGENQDKSWSIMDEIDERWPSIKAVNVRNFEKYYMGGIIE"
     gene            1748..2836
                     /locus_tag="PLU28_07960"
     CDS             1748..2836
                     /locus_tag="PLU28_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35030.1"
                     /translation="MKELEIITKVLNHSEELSKKSEKGIKEIILKENKVMYAVIKDPF
                     EHPRTIENELRKNGVFINVYRVFEDDFSSNPHKRVEMIKKSQDIAEKLFKFYFSEDFT
                     QDEYMNLKKMVFTFNKEKMRDKVILYLFLCKLKEVKSIDFLYKIELLGYSYSTRCLAK
                     LVDWMAEERKYRKKYDFGQDISIMNYGIGSVLKGNENDKSAEEAPTVNDLRNTLELVM
                     DALDEHKENIENFKQEEKAALVKEFFQTLNSKEYGYLLDNFVRCEKSIIALKKSGYEF
                     PEDVRSIPVLIRQYMKFIRDYGISEIDPSIERRLTLEQAKNSEYEGMPFMESSDEKQV
                     KTLSPGWKYKDVVISVPKYGEIIQEEGV"
     gene            2836..5478
                     /locus_tag="PLU28_07965"
     CDS             2836..5478
                     /locus_tag="PLU28_07965"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOG35031.1"
                     /translation="MGYYCAIDLGTTNTVFAYGMINPRSGNIEAKTLKMKMKSATGGI
                     ETSQLVPSCVFIENINEKEIIPVVGPYAKHMACINPQNVVRSVKSSMGTDRLYEMAST
                     KFSPSDISSFILKFVLNNAKELFKSMPDDVVITVPASFDSDMRLATIEAAKNAGIKTV
                     NEDGTERNILLDEPRAALFNFVRDIDMGNVPETVVDLSSPKNILVYDLGGGTLDVSLH
                     KVWRDENQKVKIEDYAISLHTLVGGDNFDSKIAEYLYSKIEKQVSRKNLNEFEKNRLF
                     SVLLNLAEKAKIEIAEKELNNAMMGIDEEVYAEVVQANIVDDIALDEVITKSDYEKIV
                     SDILANELTLESADSLRTSISIESKNIIYPVLDVMAKAKEKSGKDVKVDAILLNGGMT
                     KLSIIKSRLQRLFDLKPISMQDPDLSVACGGVYYHYELHNGTKFQKILNDSIGIETNG
                     QHVEHLAKAGVTLPYRSKVYDNFTIPQTGTNVIHFPIYIGSRNDTLRPNRKIAERFAR
                     FDKSLNAGDKVSVQVEINESGILSLKGFINNNENDRFVFEVDFNKKEGFADSGSINPK
                     PSRQISYKDTFENPVSKSSIKTLKNLCKNVVRVKDNNSKATFFKQIKDVEGCICRCTD
                     KNNAVNLLVSEISTYQEDLFVQRAVFIIGNLMKSTGLNNAFLDYSKKELNPMVLEVTY
                     SPQKFINKRFIIEALGKTEDSRCEEVISGVFNASHQMIKPIIESALISLGKTGTKVSS
                     FNEITGFLSSSETRQLISALWSFGKIASRSKQKPLGIKDIEPYIPQLVRIAKNHNHAC
                     VLHNCIYALVESCDSRYNGEKVSPATKNSVANLFEYVQKKYSYDLSFMRLVDVAKKAL
                     KGESLNSSEEKVLLDIRNNVSIND"
BASE COUNT         1933 a          844 c         1217 g         1503 t
ORIGIN      
        1 agatgcgtca gaaaaggaaa gaaaagagta ctttgaaaat gagtatgcaa atgaagctaa
       61 ttttcttcct gtcaaggata atcttcctaa gtactctgcc atactgattg atgaagttca
      121 ggactacgat agcagatgga tagcgatttt aagaaagttt ttccttaaag aaaacggtga
      181 gttggtcctt ttcggagatg aaaaacagaa cttatacggt agagctgttg atggagcaga
      241 aaaaaaaccc aacacaggaa ttcccggcag atggaacaag ctcaaaaaat tgaaacgctt
      301 taaaggaata ataagcccta tgattattaa ttttcaaaaa caatttctgt caggcaagta
      361 cttggttgat gaggttgaaa gctttcagac gagcattttg gattttaagt tggagtacct
      421 ttgggttgat gaacaaagca ctgtgtatgg agagcttttg cacgagattg gaaaggcaga
      481 aaacaacggt gagaacgtgg ttgttttagg tagtagtatc gagtatttaa gagttttcga
      541 aaaattttac agacaaaaga cccaaaaagc taccaagagc tcttttgaaa ctcagcaaga
      601 gtatgaagaa ataaaggcaa aattggataa agagaaaaag tccaacatga taaaaacttt
      661 atgtgaagaa ataagaaaag acagaaagtt aaatttctgg tcggaatcaa gaaatggtca
      721 tgagctaaag ataagcacaa ttcaaagttt taaaggtatg gagatgaaca acgttattct
      781 ccttatcgga gagaatgatc aagaagaact ggtatatacg ggaatcacca gagccagaga
      841 aaaattggta atactaaata atggcaatgg aaaataccat gatttttttg ccaagtacag
      901 tatattaaag gcagcataat gccgggggtg gcaatatgga gggttacttt ttggcgacag
      961 gtggaggcag tgagatagga gccagcagct actgcctttc actgggcaac aatagagtaa
     1021 ttctggactg tggaatgagg tttggcgaaa ggaagtatcc gagcatcgat tcacttttca
     1081 aagaggggat ttttccaggt tcttctcaaa ccggttcaat catcgtttcc cattctcaca
     1141 aggatcattt cggagctctt ccgtattttt atcaaaacgg ttttcaaagg atcatatcaa
     1201 ctccgcaatg taaagataac ctgataaaaa gagtatctgc ctgtttggat acttttaata
     1261 tagatcagta cctgaatttg aaacaggttt ttatgtcaat tgccgcctac gatttttttg
     1321 aaccgttttc atgcggggat ttagactgta tttttgttcc tgcaggtcat atagacggtg
     1381 cagctatgac cgttattttt tatgaagggc aaaaactggt gtatacagga gatttttctt
     1441 ttccgctttc ttctgatgag gttgaaaact catgtgcgtt tgagtttatt aaggacgcag
     1501 atgttttgat aacagagagc acctatggat acatgaagaa caaaacggtt ataagttcta
     1561 aacatgctca gtttgaagac ttatgtcatt tgatagaaaa aatctatcca aaaacaatct
     1621 ttttggttca cggggagaat caagataaaa gctggagcat tatggatgaa atagatgaaa
     1681 gatggccttc gataaaagcc gtaaatgtaa ggaactttga aaaatactac atggggggga
     1741 tcatagaatg aaggagttag agatcataac caaagtactt aatcattctg aggagttatc
     1801 aaaaaaaagc gagaaaggta ttaaagaaat tattttaaag gaaaacaaag ttatgtatgc
     1861 agttataaaa gatccgtttg agcatcccag aacaatcgaa aatgagctta ggaaaaatgg
     1921 agtatttata aatgtttaca gggtctttga ggatgatttt tcgagtaatc cacataaaag
     1981 agtagagatg ataaaaaaga gccaagatat agcagaaaaa ctttttaagt tttatttttc
     2041 agaggacttt acccaagacg agtacatgaa cttaaagaaa atggtcttca ctttcaataa
     2101 ggaaaagatg agagacaagg taatcctgta cctttttctg tgtaagttaa aggaagttaa
     2161 gagtattgat tttttatata aaatagaact tttaggatac agttattcca caagatgtct
     2221 tgccaagctg gttgattgga tggcggaaga aagaaagtac agaaaaaagt atgactttgg
     2281 tcaggatatt tctataatga actatgggat cggaagtgta ttaaaaggaa acgaaaacga
     2341 caaaagtgca gaggaagcgc ctactgtaaa tgatctgcgt aatacgctag agctggttat
     2401 ggatgctttg gatgaacaca aagaaaacat agagaacttt aagcaggaag aaaaagcggc
     2461 tttagttaaa gagttctttc aaacgcttaa ttccaaggaa tatggatatc ttctggataa
     2521 ttttgtaagg tgtgagaaaa gtattatagc tcttaaaaag tccggatatg agtttccgga
     2581 agatgttcgt tccattccgg ttcttataag gcagtacatg aagtttatac gtgattatgg
     2641 gattagcgag atagatccgt ccattgaaag aaggttgact cttgagcagg ccaaaaattc
     2701 agagtatgaa ggtatgccgt ttatggagag ttcagatgaa aaacaagtga agacgctgtc
     2761 acccggatgg aagtacaagg atgttgttat ttctgtgcct aaatatggtg agataattca
     2821 ggaggaggga gtttaatggg atattattgt gcaatagatc ttgggacaac taacacagtt
     2881 tttgcctatg gaatgataaa tcccagaagc gggaatattg aagctaaaac tcttaaaatg
     2941 aaaatgaaaa gtgccacagg aggaatagag accagtcaat tggttccttc ctgtgtcttc
     3001 atagaaaaca ttaatgagaa agagattatt ccggttgtag gaccttatgc caaacatatg
     3061 gcatgcataa atccccaaaa tgttgtacgg tcagttaaaa gctctatggg tacagatagg
     3121 ctttatgaaa tggctagcac caagtttagt ccttcggata taagttcttt tatactcaag
     3181 tttgttttaa acaatgcaaa ggaacttttc aaaagtatgc cggatgatgt tgtaataact
     3241 gtacctgcat cttttgattc agacatgcgc ttggctacca tagaggcggc aaaaaacgca
     3301 ggcataaaaa ctgtaaatga agacggaacg gagcgaaaca tacttctgga cgaacccagg
     3361 gctgcccttt ttaattttgt cagggacata gacatgggga atgtaccgga gactgttgtt
     3421 gatctgagtt ctcctaaaaa tattctggtt tatgatcttg gaggaggtac ccttgatgtg
     3481 tcgcttcaca aggtttggag ggatgaaaac caaaaggtga aaattgaaga ttatgctatc
     3541 tctctgcata cattggtagg gggagataat tttgactcta agatcgcaga gtatctttat
     3601 tctaagatag aaaagcaagt atcaagaaaa aatctcaatg agtttgaaaa aaaccggctt
     3661 ttttctgttt tgctaaacct ggctgaaaaa gcaaagatag aaatagcgga aaaagagctt
     3721 aacaacgcta tgatgggaat agacgaagaa gtatatgcag aagttgtcca ggcgaatata
     3781 gttgatgata tagctcttga cgaagtaatc acaaaaagcg attatgaaaa gatcgtttca
     3841 gatatattag ccaatgagct cacacttgaa agtgcagata gcctaaggac cagtatcagc
     3901 atagagtcta agaatataat ataccctgtt cttgatgtaa tggcaaaggc taaggagaaa
     3961 agcggaaaag atgtaaaagt agacgctatc cttctgaacg gaggaatgac caagctgagc
     4021 attataaagt cacgtttaca aagacttttc gacctaaaac caataagtat gcaagatccc
     4081 gatttatcag tagcctgcgg aggagtttac tatcattatg agcttcataa cggcacgaag
     4141 tttcagaaga ttttaaatga cagcataggc atagagacta atggccagca tgttgaacat
     4201 cttgccaaag caggggtaac tcttccttac agatcaaaag tttatgataa ctttacaatc
     4261 ccgcagaccg gtaccaacgt tatacatttt ccgatctaca tcggaagcag aaacgacact
     4321 ctccgtccta acaggaagat agctgaaagg tttgcgaggt ttgacaaaag tcttaatgcc
     4381 ggtgataagg tttctgtaca ggttgaaata aatgaaagcg ggatactttc attaaaaggg
     4441 ttcataaaca acaacgaaaa tgatagattc gtttttgaag tcgatttcaa taaaaaggag
     4501 ggatttgctg attcaggaag tataaacccc aagccttcca gacagatctc ttataaagac
     4561 acttttgaga acccggtttc gaagtcgagt ataaaaacgc ttaagaatct ttgtaaaaat
     4621 gtcgtaagag ttaaagataa caatagcaaa gcaactttct ttaagcaaat taaagatgtt
     4681 gaaggctgca tatgcagatg caccgacaaa aataatgccg tgaatcttct tgtaagcgag
     4741 atatcaactt atcaggaaga tctttttgtg cagagggcgg ttttcataat aggcaacctt
     4801 atgaaaagca caggccttaa caacgcattt ttagattaca gcaaaaaaga gcttaatcca
     4861 atggtcttgg aggtgacgta ctctcctcaa aaatttataa ataaaaggtt tatcatagag
     4921 gccttgggaa aaaccgagga cagcagatgt gaagaagtca taagcggagt cttcaatgca
     4981 agtcatcaaa tgataaagcc aataatagag tcggcgctca tttccttggg gaaaaccggt
     5041 accaaagtat cttcgtttaa tgaaattaca ggttttctta gcagcagtga aacacgccag
     5101 cttataagtg cactgtggag ttttggtaaa atcgcaagtc ggagcaagca aaaaccgttg
     5161 gggataaaag atattgaacc ttatattcct cagttggtac gtattgccaa gaaccataat
     5221 catgcttgtg tcttacacaa ctgcatatat gcccttgttg aaagctgtga cagccgctat
     5281 aacggcgaaa aagtatctcc ggcaacaaag aatagcgttg ctaatttatt tgagtatgta
     5341 caaaagaagt actcatatga tctttctttt atgagacttg tggatgttgc caagaaagct
     5401 ttaaaaggag aaagcttgaa cagcagtgaa gaaaaagttc ttcttgacat aagaaacaat
     5461 gtgagtataa acgattaaaa tcgaagcaca ccttcaa
//
LOCUS       DAOSHR010000139         5345 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_13633_length_5345_cov_5.957278, whole genome shotgun sequence.
ACCESSION   DAOSHR010000139 DAOSHR010000000
VERSION     DAOSHR010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 5345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5345
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13633_length_5345_cov_5.957278"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            213..1835
                     /locus_tag="PLU28_07970"
     CDS             213..1835
                     /locus_tag="PLU28_07970"
                     /inference="COORDINATES: protein motif:HMM:NF015515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HOG35032.1"
                     /translation="MEFGYWSLIPPVLAIGLALITKKVYISLFAGIAVGALIFTHGDI
                     ISAIGKIVTIFWDNLELKNFSSWENFNNSWNLFILFFLIFLGILVALVNRAGGALAYG
                     NWVIKKIKTREGASFATFILGVLIFLDDYFNCLTVGTVMKPLTDKHRISRAKLAYIID
                     STAAPVCIIAPVSSWVANVISQLTQSGVGKDTLAAFNPYNVFLTSVVFNSYALLTMFM
                     VVLLAFKSYDFGVMREHEIRAKKYGDLYNGGKEVSKIVDKEITPSKNGKVIDLIIPIL
                     FLIVMLIGFMLYTGGYKLLGGENSMFDAFQNMNSAKALFYSGLISVIFAVVYFAARKL
                     IPLGEYPKLFWAGFRLMLPANCILILAWGIGSIIKNDLQTGQFIGTLAAQNFPIELMP
                     AILFLIACLVAYSTGTSWGTFAILIPMAIPMVVSTGRTDLLMAMISAVLGGAVYGDHV
                     SPISDTTIMSSTGAGCNHLDHVNTQMPYATLVAGVCFVGYIIMGFTARYGLGVSGTVN
                     LLFCVPVLYILVRVLSKKASQKDDSIEDKQKA"
     gene            complement(1867..3645)
                     /locus_tag="PLU28_07975"
     CDS             complement(1867..3645)
                     /locus_tag="PLU28_07975"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOG35033.1"
                     /translation="MENSIDFKEILHRIPVGYALHKVLTDEKGKVVDYIILDVNKAFE
                     RIMGVRACDVIGKNRTELLNGKDDSMFDWINFYGEVALTGGEKEIQHYSSLWQKWFDI
                     KVFSPEKYFFITFFTDADKLVNEKEFFKRLLCSLNDGIIALDVHKKILYTNMAAGTFS
                     VILPKDAFGKDISDFFRFEQIKEKEVFLSFIDKAYEKKESFYAEFTTDVLSANNRSFS
                     LNFGISSISDPLGKFIGLNIIIKDVTQHLKYQDDLLYASFHDSLTGLYNRTFFNEKID
                     QIDTAQNLPLSLIIGDINGLKLANDAFGHLEGDRLIVNAARAIQKGCRKTDIIVRWGG
                     DEFIVLLAQTPLSSLDPIVSRIKEECLTQEKGKIELSISLGYATKTEESKSMDELLKS
                     AEEVMYTSKILESRSIKSKTMNLIITTLHERSPMDAQHSLNVSKLCKIFSKKLGLTEK
                     DCSTLEILGNIHDIGKITISDSILNKKTSLDESEWKEVKKHSEVGYHILSSTHDLAFL
                     ADCVLFHHERYDGKGYPKGIKEKEIPFFSRVLSICDAYDSMVSGRPYKKSMTKKQIIK
                     EFADCSGKQFDPELCIFFIEKVIENL"
     gene            3889..4758
                     /locus_tag="PLU28_07980"
     CDS             3889..4758
                     /locus_tag="PLU28_07980"
                     /inference="COORDINATES: protein motif:HMM:NF015944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOG35034.1"
                     /translation="MKKKFLVIVAVLSAILVSAWSVPDYYNYVNDFINVIAPAEEAEL
                     NSLAQKVEQEKGVQIAIVTVNVSQDITPKEMATELFNKWGVGSKGKDNGLVLLLALDP
                     DGTKGGNEIQIEVGVLIGVFWWFGTYNSLVSSEESVTSSWSEIDNQLLRRADLIPNLV
                     NTVKGFAAQEKTVIQSVSDARAKLAGASSAKEKMDASNELDGALSRLLVVVENYPELK
                     SDKVFISLMDELSGTENRLSVARKRYNDSVKAFNMQIKKFPSSIVAGASGFEQKDYFE
                     VPAEKKETPEVQF"
     gene            4864..>5345
                     /locus_tag="PLU28_07985"
     CDS             4864..>5345
                     /locus_tag="PLU28_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35035.1"
                     /translation="MHDWLYSFGIWGIVASLGLFLMFLYIKSLDNKSSVRKFIFAWLF
                     FTFKCSIDIFSFHVSFSGIPFISLFLIFMFYFLIFDGLCDFFDIKKFKTGISPVIFVL
                     IWLILSFIYRIPYFLTSGLVHLMSCIFCLLNSYILAKYFDKGTFEKKLLQIFFILLFF
                     "
BASE COUNT         1528 a          949 c         1080 g         1788 t
ORIGIN      
        1 gaagggtata aaaaagactc tttttatact tctgggtatg cgggaaatac ccgtataact
       61 gtcacttata gtggagagcc gtcaaatgga tgattttata gtaaatctat caaatcactc
      121 acagaaatct ctccctcgaa aagagggttt tgatttttat atgataattc tcctcacaag
      181 ggagaatttt ttaatttaat gggaggtgca tcatggagtt tggatattgg tcgctgattc
      241 caccggtttt agctataggg cttgctctta taaccaaaaa ggtatatatc tcactttttg
      301 ccggtatagc agtgggggcg cttatcttta cccacgggga tatcatttca gcaataggga
      361 agattgtaac cattttctgg gataatcttg agctgaaaaa tttttcttcg tgggaaaatt
      421 tcaacaacag ctggaatctt ttcattctgt tcttccttat ttttcttgga atactggtgg
      481 cacttgtcaa tagggctggc ggagcgcttg cttacggaaa ctgggttata aaaaagataa
      541 aaacccgtga aggagcgagt tttgcaacgt ttatcctggg tgttctcatt tttttggatg
      601 attactttaa ctgtcttaca gtaggaacag tcatgaagcc tcttactgac aagcatagga
      661 tatcgcgtgc caagcttgca tatataatag attctaccgc agcgccggta tgcataatag
      721 ctcctgtatc aagctgggtt gccaacgtta tatcgcagct tacccagtct ggagtgggaa
      781 aagatacgct cgccgcattt aatccataca atgttttcct tacttcggtt gtctttaact
      841 catatgctct tcttacaatg tttatggtag ttcttctggc atttaagagt tatgattttg
      901 gagtgatgag agaacatgag ataagggcaa aaaaatacgg tgatctttat aacggaggaa
      961 aagaggtcag caaaatagta gataaggaaa ttactccttc aaaaaacggt aaggtgatag
     1021 atcttataat tcctatactt tttcttatag ttatgctcat aggttttatg ctatatacag
     1081 gaggttacaa gcttttaggc ggagaaaaca gcatgtttga tgcttttcag aatatgaact
     1141 ctgcaaaggc tttgttctac agcggtctta tatcggttat ctttgcggta gtgtactttg
     1201 ctgcaagaaa actgattcca ttgggcgagt atcccaagct tttctgggca ggattcaggc
     1261 ttatgcttcc ggctaactgc atactcattc ttgcctgggg aataggctcc ataattaaga
     1321 atgatcttca gacagggcag tttataggaa cccttgctgc gcagaacttc cctatagagc
     1381 tcatgccggc aattcttttt cttatagcct gtctggttgc atactcaacc ggaacttcct
     1441 ggggaacttt tgctattctt attccgatgg ccatacctat ggttgtttcg acaggacgga
     1501 cagatctgct tatggctatg atttcggcag ttcttggcgg agcagtttat ggagatcacg
     1561 tttcaccgat ttcagataca actattatga gttcgacagg tgccggatgt aaccatcttg
     1621 accatgtaaa tacccagatg ccttatgcta ctttggttgc cggagtatgc tttgtgggct
     1681 atataataat gggttttacg gccagatatg gtcttggggt cagcggaacg gtaaatcttt
     1741 tattctgtgt gccagtactt tatatcctgg tgagggtact gagtaagaaa gcttctcaaa
     1801 aagacgacag tatagaggat aagcagaaag cataaatata ctataggctg tttcgtttga
     1861 aacagcctat aggttttcta taaccttttc tatgaagaaa atgcacagtt caggatcaaa
     1921 ttgttttccg gagcagtcag caaactcttt tatgatttgt ttcttagtca tagatttttt
     1981 gtatggcctg cctgaaacca ttgaatcata agcatcacag atgctcagga ctcttgagaa
     2041 aaaaggtatc tctttttctt taattccctt aggataaccc tttccatcgt agcgttcatg
     2101 gtgaaaaaga acacagtctg ccagaaaggc aaggtcatga gtcgaggata gtatgtgata
     2161 tcccacttct gagtgtttct tgacttcctt ccattcagat tcatccaatg aggttttctt
     2221 gtttaggata ctgtcactta tagttatctt acctatgtca tgaatattac ctagtatttc
     2281 aagtgttgag cagtcttttt cagtcaatcc cagttttttg ctgaaaattt tacacagttt
     2341 gcttacgttt aaggagtgct gggcatccat aggacttctc tcatgtagag tggttattat
     2401 aaggttcatg gttttactct ttatgctcct gctttcaagt attttagagg tgtacataac
     2461 ttcttcggct gattttaaaa gctcatccat gctttttgat tcttcggttt tagttgcata
     2521 tcctagggat attgaaagct ctatctttcc tttttcctgg gttaagcatt cttcttttat
     2581 ccggctaaca atggggtcaa gtgagcttaa aggagtctgt gcaagcaaga ctataaactc
     2641 atcaccgccc cagcggacaa tgatatcggt ctttctgcat cctttctgaa tagcccgtgc
     2701 agcatttact attaaacggt ctccttcgag atggccaaag gcatcgttag caagcttcag
     2761 accgttaata tctcctatta taagtgaaag cggaaggttt tgagctgtgt cgatctgatc
     2821 tattttttca ttgaaaaaag tgcggttgta aagacctgtc agactgtcgt gaaaagaagc
     2881 ataaagcaaa tcatcctggt acttcaggtg ttgggtaacg tctttaatta taatattcag
     2941 acctatgaac tttccgaggg gatcggagat gcttgatatg ccgaaattaa gtgaaaaact
     3001 tctgttgtta gctgagagta catccgttgt aaactcggcg taaaagcttt cttttttttc
     3061 atatgctttg tcaataaaag aaagaaaaac ttccttttct ttaatttgtt caaatctgaa
     3121 aaaatctgaa atatcttttc cgaaagcatc ttttggaaga ataaccgaaa aagtaccggc
     3181 tgccatgtta gtatacaaaa tttttttatg gacatccaac gctataattc catcattcaa
     3241 agaacataaa agccttttga aaaactcctt ttcattgacc agtttatctg catctgtaaa
     3301 aaaagtaata aaaaaatact tctccggaga aaaaaccttt atatcgaacc acttctgcca
     3361 taagcttgag taatgttgta tttctttctc accgccggtt aatgccactt caccataaaa
     3421 gtttatccaa tcaaacatac tgtcatcttt tccatttaaa agctcagtcc tgttttttcc
     3481 gatgacatca caggctctga ctcccataat tctttcaaaa gctttattga catcaagaat
     3541 tatgtaatcc acaacttttc ctttttcatc ggtaagcacc ttatgcaaag catatccgac
     3601 aggtatgcgg tgaagtattt ctttgaagtc gatagaattt tccatctttg cctcctgaag
     3661 agatattttt aaagttaata tatataaaaa gtgattaata ttattctata actttaaaac
     3721 ctagtatgca aacttataaa cagacgaaaa aaggcaaagg tattaaaaaa gcttttatct
     3781 atctgatata ttaaaggttt gtgcacaaaa aataaactta ggtatagtat aaatttaaca
     3841 ttatctgata taataaacct gaaaataaaa agcagaatga ggtggaggat gaaaaaaaag
     3901 ttcttggtta ttgtagctgt tttatctgcg atattggtta gtgcatggag tgttcctgat
     3961 tattataatt atgtcaacga ttttataaat gtgattgcgc ctgccgaaga agcagagctg
     4021 aatagtcttg cccaaaaagt tgagcaagag aagggagttc agatagcaat cgttaccgta
     4081 aatgtttctc aggatataac gcccaaggag atggccactg agctcttcaa caaatggggc
     4141 gtaggcagca aaggcaagga taacggactt gttttgcttt tggctcttga tcctgacgga
     4201 accaaaggcg gaaatgagat acagattgag gtaggcgttc ttataggagt tttctggtgg
     4261 ttcggtacct ataactcctt agtaagttca gaagagtctg ttacttccag ttggtcggaa
     4321 attgacaacc agcttctgcg cagggcagat ctcattccta accttgttaa tacggtaaaa
     4381 ggttttgcag cccaggaaaa aacagttata cagagtgttt cagatgcgag ggctaaactt
     4441 gccggggctt caagcgcaaa agaaaagatg gatgcgtcga atgaacttga cggagcactt
     4501 tccagacttc ttgttgttgt tgaaaactat ccggagctta aatcagacaa ggtattcata
     4561 tctctcatgg atgagctgtc gggtacagaa aacagattgt cggtggcaag aaagagatat
     4621 aatgattctg taaaggcatt caatatgcag attaagaagt ttccttcaag tatagttgcc
     4681 ggtgcctcgg ggtttgaaca gaaagattat tttgaggttc ccgcagaaaa gaaagaaact
     4741 ccagaagttc agttttaatt taccggccgt caggccggtt ttgttttgct tatggatggt
     4801 caattaacga ataaaacaac tttttatgat ataataataa caaaacagtg cggagggatt
     4861 attatgcatg attggcttta ctctttcggc atctggggaa tagtagcttc tttgggctta
     4921 tttttgatgt ttttgtacat aaaaagtctg gataacaaaa gctctgtgcg taagttcatc
     4981 ttcgcatggc ttttttttac ttttaagtgc tcaattgata ttttcagttt tcatgtaagc
     5041 ttttctggca ttccttttat ctctcttttt ttaattttca tgttttattt tttaattttc
     5101 gacggattat gtgatttttt tgatataaag aagtttaaga ctggaataag tcccgtgata
     5161 tttgttttaa tatggcttat tttaagtttt atataccgta ttccctactt tctgacctcc
     5221 ggacttgttc atcttatgtc ctgtatattc tgtctgctga attcttatat acttgcaaaa
     5281 tacttcgata aaggcacatt tgagaaaaaa cttcttcaga ttttttttat tctgcttttc
     5341 ttttt
//
LOCUS       DAOSHR010000140         5341 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_13648_length_5341_cov_4.994325, whole genome shotgun sequence.
ACCESSION   DAOSHR010000140 DAOSHR010000000
VERSION     DAOSHR010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 5341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5341
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13648_length_5341_cov_4.994325"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2249)
                     /gene="pheT"
                     /locus_tag="PLU28_07990"
     CDS             complement(<1..2249)
                     /gene="pheT"
                     /locus_tag="PLU28_07990"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOG35036.1"
                     /translation="MRLSIEWISEYIKVLKSPAELSKQIKLHTTNVESVQAVSRDLCN
                     VIVGKVEQVQKHPDADKLIVCRVNMGNEYKQILTADLSVKEGDVIPVAVNGAKLASGM
                     EIKDRKMRGIESQGMFCSLSELGLEEKSDGVYKLKEEVPLGTDFIEYFKLKDDIVEIE
                     VFPNRPDLLSYIGAAKELQVIKAAEGFKLPEYDELSRTDTFDVSINYEGCKRYTALEM
                     KNIKVGSSPLWLVRRLAACGIRSINNIVDITNYVMLESGHPVHAFDLDVIGRKIVVRK
                     AQKGEKTVLLDEKEYELNGTETLITGEKGILALGGVMGCQNSGITSETRNILLEVAYF
                     DPVITRKSAKYHKLSTDASYRFERGVDPNDAMFVMGRLAYLIKTIACGTVHEGVTDVY
                     PIVEMSKCIHLRKWYVDERLGKVIPIEEIESILGRYGFEYKVQGDGWDITVPTKRPDL
                     SIEEDLVEEVARAYGYYNISSALPVVKMFKGSKSEFVSFKDDAAYTLRQEGYHEAMTY
                     SMMNIKNMWMDKSDIRILNPLSAEYEYMRPILVYGILESASYNFRNQNKDIKLFEIGK
                     TFVRDSSFDTGIKETTKAAIVACGRENPYDYTDKREVSFFTVKGALEGLFESLNLNAR
                     YERAEITGLSKAQSADIFIGDKKIGFMGLLEKDISDKLYDIKSSVYIAEVDMDMLFEM
                     KKPISKKLKTFDYPAIKREYSFIAPVQAKFEDIHSIIQSSGKIIESINIFDIYKGKGI
                     DPDKMSITIT"
     gene            complement(2260..3279)
                     /gene="pheS"
                     /locus_tag="PLU28_07995"
     CDS             complement(2260..3279)
                     /gene="pheS"
                     /locus_tag="PLU28_07995"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOG35037.1"
                     /translation="MTIEKKKILDALEADLLNVSNIQDLQNIKARYLGKKGTVTDLMK
                     EIGRIPPESKKEYGMLVNELKNDIESLLETKEEQVRELEKKSLETKNWVDVSLPGARR
                     AVGSEHLVMKAIREISEVFINMGCTIVEGPEIEIPWYNFDALNIAPWHPARDMQDTFY
                     IESEKNLILRTHTSPVQVRTMLKSKPPLAIISPGRVYRKDEIDATHAAMFNQVEGLYI
                     DRQVTVAHLKMFLEILAKSMFGDRMQVLLRPSYFAFVEPGFEVDVSCMNCGGKGCNVC
                     KNTGWIEILGAGLVHPNVLRNSGIDPNEWQGFAFGMGAERIALLKYGVNNLREFTRND
                     IRVIE"
     gene            complement(3251..>3727)
                     /locus_tag="PLU28_08000"
     CDS             complement(3251..>3727)
                     /locus_tag="PLU28_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35038.1"
                     /translation="FFIVLCGYMLKPFSPGRYMLAGFKYYSRILSVIISFAVFCAVAY
                     FSSFISFFGIIDGLFPVIIGVVAVLAGNIFSYRILQWIISIAVLSLIFFDFASEYRYF
                     ILGGSFVLNIIVGYCMIKTHSYIRTIGSNTRKKENKNKEESVKEDNFYDNRKEEDS"
     assembly_gap    3728..3827
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3863..4477)
                     /locus_tag="PLU28_08005"
     CDS             complement(3863..4477)
                     /locus_tag="PLU28_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35039.1"
                     /translation="MPIEESGASTTVIRIVEPNNGFPEMVIEKGQSSGIFTSKVKETG
                     KLFFQILCEPNRYCRKLTINEIVANQSVWLGRAFTHLVIPDMWNESLLWILKNKPRDK
                     HFVIIAAGEKSVRQKDLSEISRLVSEYTKDGAVLTITDSRELIEAVCDSVTDEKGEEI
                     FFETGPFFFDLSDNTLIEDIGKIEQNIKDVRIKKILLENKLPER"
     gene            4609..5271
                     /gene="fsa"
                     /locus_tag="PLU28_08010"
     CDS             4609..5271
                     /gene="fsa"
                     /locus_tag="PLU28_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HOG35040.1"
                     /translation="MKIFLDTANIQEIKKGMEWGIIDGVTTNPTLVSKENAVFEDRIK
                     EICETVKGPVSAEVTAMDTEGMVSQAQELAKISPYVVVKIPMTTEGIKAVKILSSKGI
                     NTNVTLIFSPLQAMLAAKAGATYVSPFIGRLDDIGHEGMELVTQISEIFFNYDFATQI
                     IVASVRHPEHVLQAAMCGADISTIPFDVLKKLFNHPLTDIGISKFNEDWKKYQDYLKS
                     KK"
BASE COUNT         1537 a         1190 c          845 g         1669 t
ORIGIN      
        1 gttatggtta tgctcatctt atccggatcg attccttttc ctttatagat atcaaagata
       61 tttatgctct caattatttt tccgctggac tgtataatgc tgtggatatc ttcaaacttt
      121 gcctgaacag gagcgatgaa tgaatactcg cgttttatag ctggataatc aaaggtttta
      181 agctttttgg atatgggttt tttcatttca aagagcatat ccatatctac ttcagctatg
      241 taaacactcg actttatatc gtagagtttg tctgaaatat ctttttccaa aagacccatg
      301 aatcctattt ttttatctcc tataaatata tcggcacttt gggctttgct taatcctgtt
      361 atttcagccc tttcatatct ggcatttaaa ttaaggcttt caaaaagacc ttcgagcgct
      421 ccctttacgg taaaaaacga tacttccctc ttgtcggtat aatcataggg attttccctt
      481 ccgcaggcta ctattgcggc tttggtagtc tccttgatgc ccgtgtcaaa agaactgtct
      541 cttacgaagg tttttcctat ttcaaataac tttatatcct tgttctgatt cctgaaatta
      601 tatgatgccg attcaagaat accatacacc aggataggac gcatatactc atactcggca
      661 gaaagagggt taagaattct gatatccgac ttatccatcc acatattttt tatattcatc
      721 atcgagtacg tcatagcttc atgatagccc tcctgtctga gggtgtaagc tgcatcatct
      781 ttgaatgaaa caaactcgct ttttgatccc ttgaacattt ttactacagg taatgctgaa
      841 cttatattat aatatccgta tgctcttgca acttcttcta caagatcttc ttctattgaa
      901 agatccgggc gtttagtcgg aacggttata tcccatccgt cgccctgtac cttatattca
      961 aaaccatatc ttccgagtat actttcaatc tcctctatag gaatgacctt accaagcctt
     1021 tcatcaacat accacttacg caggtgaatg cacttggaca tctccacaat cgggtataca
     1081 tcggtaacgc cttcatgaac agtaccgcaa gctatggttt ttataagata agcaagtctt
     1141 cccattacaa acattgcatc atttggatct acacctcttt caaaacgata tgaggcatct
     1201 gttgaaagct tatgatactt agcagacttt ctggtaatta ccggatcgaa gtacgcaacc
     1261 tccagcaaaa tatttctggt ttcggaagtt ataccgctgt tctgacaacc catcacacct
     1321 ccgagagcaa gtatgccctt ttcaccggta ataagggttt cagttccgtt gagttcatac
     1381 tctttttcat caagcaaaac tgttttctca cctttttgtg cttttcttac aacaatcttt
     1441 cttcctataa catccaaatc aaaagcatgt acaggatgtc cgctttcgag catcacgtaa
     1501 ttggttatat caacgatatt atttatgctt cttataccac aggcggcaag tcttctgaca
     1561 agccacagag gtgaagatcc aacctttatg tttttcatct caagtgcagt atacctttta
     1621 catccctcgt aatttatgct tacatcaaaa gtatctgtcc tgctaagctc atcatactca
     1681 ggtaatttga agccttccgc cgcttttata acttgcagct ccttagccgc accgatatac
     1741 gaaagcagat ccggtctgtt gggaaatacc tctatttcaa caatatcatc cttaagcttg
     1801 aaatactcta tgaagtctgt ccccagagga acttcctcct ttagcttgta tactccatca
     1861 gatttttctt caagtccaag ctcagaaaga gaacaaaaca ttccctgaga ctctatgcct
     1921 ctcatctttc tgtcttttat ttccatcccg ctcgcaagct ttgcaccgtt tacggctaca
     1981 ggtattacat ctccttcttt aacagaaaga tcagccgtaa gaatctgttt atactcattt
     2041 cccatgttca ccctgcatac gataagttta tccgcatccg gatgcttttg tacctgttcg
     2101 acctttccga caattacatt gcacagatcc ctgcttactg cctgcacaga ctctacgttt
     2161 gttgtatgga gctttatctg tttggacagc tcggccgggc ttttaagaac ctttatatat
     2221 tcagaaatcc attcaattga aagtctcata agccacccct tattctatta cccttatatc
     2281 gtttctggtg aactctctca gattgttcac accatacttc aaaagtgcta ttctttccgc
     2341 tcccattcca aaagcaaaac cctgccattc attgggatct ataccgctgt tcctaagaac
     2401 attcggatgt acaagccccg ctcccagtat ctctatccag cctgtattct tacagacatt
     2461 gcaacctttt ccaccacagt tcatacagct tacatccact tcaaaaccag gttccacaaa
     2521 agcaaagtag cttggtctca gaagcacctg cattctgtct ccgaacatag atttggcaag
     2581 aatttcaaga aacatcttaa ggtgagctac cgtaacctgc ctgtctatat aaagaccctc
     2641 aacctgatta aacatagccg catgagtcgc atctatctcg tctttcctgt agaccctgcc
     2701 gggagatatt atagccaaag gcggcttact tttgagcatg gttcttacct gtacaggtga
     2761 cgtatgagtc ctgagaataa gattcttttc ggattctata taaaatgtat cctgcatatc
     2821 tcttgcagga tgccatggag ctatattcaa cgcatcaaag ttataccatg gaatctctat
     2881 ttccggacct tcgactatgg tacaacccat atttataaaa acttccgata tctctcttat
     2941 ggctttcatt acaaggtgct cactacctac agctctacgg gcacctggaa gtgacacgtc
     3001 aacccagttt tttgtttcca gagatttttt ctccagttcc ctgacctgct cttcctttgt
     3061 ttcaagaagt gattctatat catttttaag ctcattgacc agcatcccgt actctttttt
     3121 actctccggc ggaattctgc caatctcttt cataagatct gttaccgtac cttttttgcc
     3181 cagatatctg gcctttatat tttgcagatc ctgaatattt gaaacattga gaagatcagc
     3241 ttcaagcgca tcaagaatct tcttcttttc tattgtcata aaaattatcc tccttaactg
     3301 attcttcctt atttttattt tctttttttc ttgtattaga acctatcgtc cttatatagc
     3361 tatgagtttt tatcatacaa tacccaacta taatattcaa cacaaaactg ccacccagta
     3421 taaagtacct gtactccgaa gcaaaatcaa agaagatcaa agataaaacc gcgatactta
     3481 taatccattg caagattctg taagaaaaaa tatttcctgc aagaacagcc acaactccta
     3541 taataacagg gaaaagacca tctataattc caaaaaaact tataaaagag ctgaaatatg
     3601 cgacagcaca gaatacggca aaagaaataa ttaccgacaa aatccggctg taatacttaa
     3661 agcccgccag catatacctt cccggagaaa atggctttag catataacca caaagaacta
     3721 taaaaaannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntga tatactttgg
     3841 catatttatg gcctgcttta atttatcttt ccggaagctt gttttccaga agaatttttt
     3901 tgattcttac atcctttata ttttgttcta tcttacctat atcctcaatc aaagtattat
     3961 ccgaaagatc aaagaaaaat ggccctgttt caaaaaatat ttcttcaccc ttttcatctg
     4021 ttacactgtc acaaaccgct tctataagtt ctctgctgtc ggttatagtc agaaccgcac
     4081 catctttggt atattcagat accaaacgtg atatttccga aagatctttc tgcctgaccg
     4141 atttttcacc ggctgctata atgacaaaat gcttgtcacg cggtttattc ttaagtatcc
     4201 acaataaaga ttcattccac atatcaggaa taacaagatg cgtaaacgcc cgacccagcc
     4261 ataccgactg attggcgact atctcgttta tagtgagttt tctgcagtac ctgtttggtt
     4321 cgcagagtat ctggaaaaaa agctttcctg tttccttgac ttttgatgta aagattcccg
     4381 atgattgacc tttttctata accatctcgg gaaatccgtt gttgggttcc acaattctta
     4441 ttactgtagt tgatgcaccg ctttcttcta ttggcaaaaa gtattacctc cgtatcaaaa
     4501 ggctatttta gactaattta cattatacca aaaaattatt aaaaaataaa atttacattc
     4561 tgatgctaaa atactacagg aacaaattaa tttaaggagg tgcaccaaat gaaaatattt
     4621 ttggatacag caaatatcca ggagattaaa aaaggcatgg agtggggcat aattgacgga
     4681 gtaaccacta atccgacact tgtttccaaa gagaatgcag tctttgaaga cagaataaaa
     4741 gagatctgcg aaacagtaaa gggccccgtc agcgcagaag taaccgcaat ggatacagaa
     4801 ggaatggtaa gtcaggcaca ggagcttgca aaaataagtc cttacgttgt agttaaaata
     4861 cctatgacga cagaaggaat aaaagcagta aaaatactaa gttcaaaagg cataaataca
     4921 aacgtcaccc ttattttcag tcctctgcag gctatgctcg ccgccaaagc aggagcaaca
     4981 tatgtaagcc catttatagg cagacttgac gacattggtc atgaaggaat ggagcttgtt
     5041 acacagatat cagaaatttt ctttaactat gattttgcaa ctcagataat tgttgccagc
     5101 gtgagacatc cggaacacgt actccaggct gcaatgtgtg gtgctgatat atccaccatt
     5161 ccgtttgatg tgctgaaaaa gcttttcaac caccctctta cggatatcgg aatctcaaag
     5221 ttcaatgaag actggaaaaa atatcaggat tatcttaaat caaagaagtg aaaaaaaaag
     5281 cacaccttga aggtgtgctt cgattttaat cgtttatact cacattgttt cttatgtcaa
     5341 g
//
LOCUS       DAOSHR010000141         5271 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_13901_length_5271_cov_4.545437, whole genome shotgun sequence.
ACCESSION   DAOSHR010000141 DAOSHR010000000
VERSION     DAOSHR010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 5271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5271
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13901_length_5271_cov_4.545437"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="PLU28_08015"
     CDS             complement(<1..183)
                     /locus_tag="PLU28_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35041.1"
                     /translation="MKQNILGIIDALGLKSLPILRYFLDNIDVKDNEMQEYIRGLGYY
                     YNFEYEKAEQLFSTGFN"
     gene            352..1197
                     /locus_tag="PLU28_08020"
     CDS             352..1197
                     /locus_tag="PLU28_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35042.1"
                     /translation="MKKILLAVLILLSVMSFAAEKVCLINLDKNLSIGDMVYDQQNLY
                     YLSFDPSTILTEVVVFDYNEKIFTTRAIYKEEKYGNQIYVSDKYGNPRMHNFYGGQSN
                     AGIFVDSKNIYVGISKDFYVFDKNLKLKTVFEDMNTRFLRVKSINGKIYVIQDMVISI
                     YENNSLVTLYTDIPEIDLFSDMFIIDEAVFVVGRKYEERQNSIFKFNGDKFSKVYCSD
                     DDCGIIKADFYNDRIILQGKKLVKLSKEFVEISSLENPKIEYFMPCENFYVENINNVF
                     YKVLW"
     gene            complement(1357..2772)
                     /locus_tag="PLU28_08025"
     CDS             complement(1357..2772)
                     /locus_tag="PLU28_08025"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HOG35043.1"
                     /translation="MQAGIGYEIFPDRFFSSCPKNETREWNLELPLPEEFKEKKYKYG
                     TVFYGGDIKGITKKLDYIAGLSVDFIYLTPIFSSPSNHRYDCSDYFTIDPMLGTEKDL
                     KELCSAAMENKIRIVLDIALNHVGDTHPWFIKAAKGLKEKEFFRQINGSFTFWSNSKN
                     LPELALENSELIDLLWRSEDSVMKKWMKTGVNDWRLDCAYELGQELLSDISKNISSHS
                     EHHIYAEAWTYPKKWLLNGHTHGIMNYYFKNIIDDFLNNTIDGSQTAEILSYTVKDCT
                     EEKLFSCWNILSTHDTPRVKNQYTDLWKLAVVMQFTLPGSPLIYYGEEIGMTGENDPY
                     CRQPMRWELMESENETFNFYLKITKLFKDSPALREGKFTRAFSNNQNILAYSRSTSKI
                     DEYRLIVINPTHKDQEFTAYTTESFLMNRAQMQDIFSQERYTIVCSSIKGTIDPGSFL
                     ILQPLIDGTYTPYKRLINSNY"
     gene            complement(2785..>3057)
                     /locus_tag="PLU28_08030"
     CDS             complement(2785..>3057)
                     /locus_tag="PLU28_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35044.1"
                     /translation="TLLDGMPKLDFAQELPNIYLEYYNYYSEKKIDRKYFDELMLKLS
                     DRIFFFTGVIDNIMLAYSDDSSLPENVKAQMRKEFIERVDTYFAAQ"
     assembly_gap    3058..3157
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3158..3695)
                     /locus_tag="PLU28_08035"
     CDS             complement(<3158..3695)
                     /locus_tag="PLU28_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35045.1"
                     /translation="MLRITKYIKKLEKFDIQIIKKENLGDPFKYRHYGEKDFEIINLE
                     YLNGSKIKKASFVLKKYFRTDIPMILSSDNSFREIQFAKSKLMSDFKSFFSIPIIDVD
                     SEKKIIYMINKGDELKRFGPPEIPTYEKMSILVSRLAKKDAFINRNVEPFLGTYLTWV
                     LNLKKLFCEVCNADESSEL"
     gene            3972..5123
                     /gene="aspC"
                     /locus_tag="PLU28_08040"
     CDS             3972..5123
                     /gene="aspC"
                     /locus_tag="PLU28_08040"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004069 - L-aspartate:2-oxoglutarate
                     aminotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase"
                     /protein_id="HOG35046.1"
                     /translation="MLFSKNILAVEPSLTLALNAKAIELEKQGVDLVKLTAGEPDFPT
                     PEPICEAAFKAIKEGKTKYTDAKGILDLRKKISEYLNKRLDIKYDPNDIVVTNGGKQA
                     IYNILLAMINPGDEVMIFDPSWVSYDAQIKMVGGLPVHVPLDEEEGFLPTESKIRRYL
                     SPKTKVIMINTPNNPTGVVYPKSTLEMIAKISKENEILVLSDEVYELLVYDGQFTSIA
                     TIDDMKERTAIVNAFSKTWSMTGWRVGYCTGPQKLMKEVSKIQSHATSNINTPSQYAA
                     MVAFDYDVMPMYQEFKNRRDYITSRLSKMGLKFVKPAGAFYVFINVEEFGMEDSVFAQ
                     KLLEDAKIAVVPGSGFYKKGYIRISFATSMKMLEKAADRLEKFVGELRK"
     gene            5125..>5271
                     /locus_tag="PLU28_08045"
     CDS             5125..>5271
                     /locus_tag="PLU28_08045"
                     /inference="COORDINATES: protein motif:HMM:NF025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HOG35047.1"
                     /translation="MKADVLIAEIGSTTTVLTALTGINGKNPRIQAQGEHYTTVSEGD
                     VTIGI"
BASE COUNT         1695 a          846 c          919 g         1711 t
ORIGIN      
        1 gttaaaacct gttgagaaaa gttgttctgc tttttcatat tcaaaattat agtagtagcc
       61 aagtcccctt atatactctt gcatctcatt gtcttttaca tcaatattat ccaaaaaata
      121 tctaagtata ggcagagatt ttaagcctaa agcgtcaata atacctaaga tattttgttt
      181 catagtatcc ccctcatttt ttaaaagacc aataagaccc cacaaaaaaa tatttttact
      241 atataatata attagtataa aatattttat catataatta ttgttttttt tgctatcatt
      301 tctaaaaaaa caattatatg atattggata tccaatataa ggaggataag gatgaaaaaa
      361 atattattag cggttctgat acttctatca gtgatgagtt ttgcagcaga aaaagtttgt
      421 ttgatcaatc tggataagaa cctttctatt ggagacatgg tttatgatca gcagaatctt
      481 tactatctgt cgtttgatcc atctactata cttacagaag ttgttgtgtt tgattataat
      541 gaaaagattt tcactacacg tgcaatatac aaagaagaaa aatacggcaa ccagatatat
      601 gtgtccgata aatacggaaa tccgcgaatg cataattttt atggtggtca gagtaacgca
      661 gggattttcg ttgattcaaa aaatatatat gtaggtataa gcaaggattt ttatgttttt
      721 gataaaaacc ttaaactgaa gacagtgttt gaagatatga acacacgttt cttaagagtt
      781 aagtcaataa atggaaaaat ttatgttatt caggacatgg tcataagcat ttatgaaaat
      841 aacagtttag taacgcttta tacagatatt cctgaaatag atttattcag tgatatgttt
      901 atcatagatg aagctgtctt tgttgtagga agaaaatatg aagaaaggca aaacagtatt
      961 tttaaattta acggtgataa attcagcaaa gtttattgct cagatgacga ttgtggtatt
     1021 atcaaagctg atttctataa tgacaggata atacttcagg gcaaaaaact tgtcaaactt
     1081 tcgaaggagt ttgttgaaat aagtagctta gaaaatccta agatagagta ttttatgccg
     1141 tgtgagaatt tctatgtaga aaacataaac aatgtgtttt ataaagtttt atggtaagga
     1201 aaaacgggtt ataaaacaaa atctttttag atgtactata agccaagtca ggcgctttag
     1261 ccttgacttg gcttttgagt ttgtgtacgc tacttgtttg tctgtatagg ttggattatt
     1321 tttaatataa ataaagaata ttacagttaa acaacgctaa taatttgaat ttataagcct
     1381 tttatacgga gtataggttc cgtcaatcag gggttggagt atgaggaagc tacccgggtc
     1441 aatggttcct tttatagaag aacatacgat cgtatatcgt tcttggctaa aaatatcctg
     1501 catctgtgca cggttcataa gaaaagattc tgttgtgtat gcagtaaact cttgatcttt
     1561 atgagtaggg ttgataacta taagtctata ctcgtcaatc ttgcttgttg acctactata
     1621 ggcaagaata ttttggttgt ttgaaaatgc tcttgtaaac tttccttccc gtaaggcagg
     1681 agagtcttta aaaagcttgg ttattttaag ataaaaattg aaagtttcgt tctcgctttc
     1741 catcaattcc catctcatgg gctggcggca gtaaggatcg ttttctccgg tcattcctat
     1801 ttcttcccca tagtatatca aaggacttcc cggtaaagta aactgcatta ctactgcaag
     1861 cttccaaaga tcggtatatt gatttttaac tctcggtgta tcgtgtgttg atagtatatt
     1921 ccaacatgaa aagagctttt cttctgtaca gtctttgacg gtataagata atatttctgc
     1981 ggtctggctc ccatcaattg tgttattcaa aaaatcatct atgatatttt taaagtagta
     2041 attcattatg ccgtgagtat gtccgttcaa aagccatttt ttaggatatg tccaggcttc
     2101 ggcataaata tgatgctctg agtgagatga aatgtttttg cttatgtcac tgagcagttc
     2161 ttgacccagt tcataggcac agtcaagccg ccaatcgttt actcctgttt tcatccattt
     2221 tttcattacg gaatcttcgc tgcgccaaag caagtcaatc aattcagagt tttccaaggc
     2281 aagttctgga aggttttttg aattggacca aaatgtgaaa cttccgttta tttgtctgaa
     2341 aaattctttt tctttcagtc cttttgcagc ttttataaac catgggtgag tgtcgccaac
     2401 atgattcaga gctatatcaa gcactatgcg gattttattt tccatagccg cgctgcacag
     2461 ttcttttaga tctttttccg ttcccagcat aggatcaata gtaaagtaat cggagcaatc
     2521 gtatctatga ttggatgggg aactgaaaat aggagtaagg tagataaaat caactgaaag
     2581 cccagctata taatcaagtt ttttagtgat acctttgata tcgccaccat aaaatacagt
     2641 tccatatttg tatttttttt ctttgaactc ttcaggtaaa ggaagttcca aattccattc
     2701 ccttgtttca ttttttggac aggaagaaaa aaatctatcc ggaaaaattt catatcctat
     2761 tccagcctgc atatatcacc tcggttactg tgccgcaaag tatgtgtcta ctctttcaat
     2821 aaactcctta cgcatctgag cttttacatt ttccggcaaa gaactatcat cgctgtatgc
     2881 cagcataata ttatcaataa cgcctgtaaa gaaaaaaatt ctgtcactga gtttaagcat
     2941 aagttcatca aagtactttc ggtcaatctt tttttcggag tagtagttgt aatactccag
     3001 atatatattc ggaagttcct gtgcaaaatc cagtttgggc ataccgtcaa gcaaggtnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaaa gctcagaact ttcatctgcg
     3181 ttgcatactt cacaaaatag tttttttaga tttaataccc atgtaagata tgttccaaga
     3241 aatggttcaa catttctgtt aataaatgca tccttttttg ccaatctgga tacaagaata
     3301 gacatcttct cgtatgtcgg aatctcagga gggccaaatc gtttcaattc atcaccttta
     3361 tttatcatat aaataatctt cttttcagaa tccacatcta taataggtat tgaaaagaaa
     3421 cttttgaaat cactcataag ctttgatttt gcgaactgta tctctctgaa ggagttatca
     3481 gaggatagga tcatcggaat atctgttcta aagtatttct ttagcacaaa gcttgctttt
     3541 tttatttttg aaccgttaag gtactctaag ttgattatct caaagtcttt ctctccataa
     3601 tgcctgtatt taaaaggatc tcctaggttc tcttttttta ttatctgaat atcaaacttt
     3661 tcaagtttct taatatactt ggttattctt agcaaaatat cacctccata tatgccaata
     3721 tattatacac ataatttttt aaaactaccg cagagtaagt cagaacagta ttgtaataaa
     3781 aaatcttttt tttagaaaca cctttcaatt tacaatgaat atcaaagtta tttgtctgaa
     3841 aaacggcttt tcttaggcta cattaaaact aacaagtttg ttttgagaga aaaaataata
     3901 tagaatttaa gtgaaaaaaa tttcatgaaa gtactttcaa ataaattata ccttgaagga
     3961 ggagatcttg aatgcttttt tcaaaaaata ttttagcagt tgaaccttct cttactcttg
     4021 ctttaaatgc aaaagccata gaacttgaga agcagggtgt tgatctcgtt aaactgacag
     4081 cgggtgaacc ggactttcct acacctgagc ctatttgtga agctgcgttc aaagctataa
     4141 aggaagggaa aaccaagtat accgatgcaa aaggtatcct tgatctcaga aagaaaattt
     4201 cagagtatct gaataaaaga ctggatatta agtatgatcc gaatgatatc gttgtaacaa
     4261 acggtgggaa acaggctata tataatattt tgctggctat gataaaccca ggtgatgaag
     4321 taatgatttt tgatccttca tgggtaagct acgatgccca aataaaaatg gtaggcggtc
     4381 taccggttca tgttccgctt gatgaagaag aaggatttct tcctacagaa tcaaaaatta
     4441 gaagatacct ttcacctaaa acaaaggtta tcatgattaa tactccgaat aatcctacag
     4501 gagtcgttta tcccaaatcc acacttgaaa tgatagcaaa aatatcaaaa gaaaacgaaa
     4561 tacttgtttt aagcgatgaa gtatatgaac tgttggtgta tgacggacag tttacttcaa
     4621 ttgccacaat agacgatatg aaggaaagaa cagctatagt taatgcattc tcaaaaacat
     4681 ggtctatgac cggatggaga gtaggttact gtacaggacc tcagaaactc atgaaggaag
     4741 tatctaaaat acagtcacat gctacatcaa acatcaacac tccttcacag tatgcagcaa
     4801 tggttgcttt tgactatgat gttatgccca tgtatcagga gtttaaaaac agaagggatt
     4861 acataacctc aagacttagc aaaatgggtc tcaagtttgt aaaacctgcc ggagcatttt
     4921 atgtgtttat aaatgttgag gagtttggaa tggaagactc agtttttgcc caaaaacttc
     4981 tcgaagatgc aaaaatcgcg gttgtaccgg gttccggatt ctataaaaaa ggttatataa
     5041 gaatatcgtt tgctacttca atgaagatgc tcgaaaaagc tgccgacaga ctggaaaaat
     5101 ttgtaggcga actgagaaaa taacatgaaa gccgatgttc tgattgctga aataggaagt
     5161 acaactacag ttcttaccgc acttaccggt attaacggta aaaatccaag aattcaggct
     5221 cagggagaac attataccac ggtttccgaa ggcgatgtta ctattggtat a
//
LOCUS       DAOSHR010000142         5235 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_14037_length_5235_cov_6.089382, whole genome shotgun sequence.
ACCESSION   DAOSHR010000142 DAOSHR010000000
VERSION     DAOSHR010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 5235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5235
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14037_length_5235_cov_6.089382"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            92..2086
                     /locus_tag="PLU28_08050"
     CDS             92..2086
                     /locus_tag="PLU28_08050"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOG35048.1"
                     /translation="MKKYIILGITLFLINCNIFSSEQLRVLGDKNFPPYEFLNEDGQI
                     DGFLVDLMFSLSNETGRMFDIQLKDWTQAQEEVLSGKADFLMGMKVSENRLRNFDFGD
                     PYLKMDMVVFTRYGSAIRSIKDSTELIAAVEESTASHDWLVKNGYKNILKASDYKTAL
                     EYLLNESADVLVVGDYNAAQYFISAQDAGKKLVLTDRLDSNYYAFAVKKGNKELLKEL
                     NQALSRIKKDGTFDKIYKKWFGEDLYNYSRMKDIYNGIFMAIFAVLLVMIVFLMILFI
                     KLRRRTREIQTKNIELQTAYRHTEDLTLKLQTLIDISSKTIFKKGLSEQTFFDELLKA
                     SFKIVPEADYGSISLVDEDRWCFVSAVGHDIEKLRELDLKAEYMIDARDIKIVNHIFE
                     ENRKILPKELFEKINEASVRVKESMIVSMEIENRLVGNISLDIGENNPYVFTEESKKA
                     LRSFANIAASFMAYKNFLSMKNRVQEEVISAVLNILDMHDPYTKGHSKNVAILSRSIA
                     AEMGYAENECEKIYWAGLVHDIGKILISVGILNKPAKLTVDEYDEIKKHPVYGYEFFN
                     RNQGLKDIAEIVLYHHERYDGRGYPKGLREGEIPVFSRIIAVADAFDAMTNNRSYRRA
                     VSTDEAVAELKKESAKQFDPHIVDIAVRIIRSEAGLNIFS"
     gene            complement(2070..2570)
                     /locus_tag="PLU28_08055"
     CDS             complement(2070..2570)
                     /locus_tag="PLU28_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35049.1"
                     /translation="MYNSRNGSIWISLLLIFLGVTILLGVIFPQVKVWALIWPLFILI
                     PGIAFELSFFHRPAPLRAGLLVPGGILCTIGLLFLFLGFTDYAYMAFLWPVFILAPAV
                     GLFQLYLFGMRIPALMIPVSILSIISAFFFMLSMSSTAPGALVFGAFLIFVGIVILSG
                     VFRKKY"
     gene            complement(2604..3587)
                     /locus_tag="PLU28_08060"
     CDS             complement(2604..3587)
                     /locus_tag="PLU28_08060"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HOG35050.1"
                     /translation="MLITALFSAFLDNLITILFISPIIFLITDTLEIDAAPFIMLTIF
                     IDNIGGMSTLIGSPLNIVLGSISKLDFTQFMKIMSVIAFITFISTFTIFRKKYTKAQT
                     AEFSEKLKRLGNMKPEKAITNKKLLLISIIVFALVLTGFIFSSAIKINSALIAVFGGL
                     ILLFVFNHDFASFSSRIDWDTMFFYAGLFSISYALDKIGVTQSIAWIFKPLIGAPVIL
                     MLCFMWLSAFVIPFLSAVPGTLLLAPVVKILIGYGAPAELWYAYAVGANLGSNFTPLG
                     AVQNIVGVSLLEKHGGKSLSFSQFMNYSKKTFLVSLILGTFFVLGLYFVKF"
     gene            complement(3553..3885)
                     /locus_tag="PLU28_08065"
     CDS             complement(3553..3885)
                     /locus_tag="PLU28_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HOG35051.1"
                     /translation="MLNGALVIVIAIVAYYFIIFSKKVKKSVTTLLLSIILYIFNPVN
                     TLTLENIGKIISFETLGILFGMMIIVEFLKESGFFTFSAVWVIKASKVLGSFVFAHAH
                     YRSFFSFS"
     gene            3994..4881
                     /locus_tag="PLU28_08070"
     CDS             3994..4881
                     /locus_tag="PLU28_08070"
                     /inference="COORDINATES: protein motif:HMM:NF015132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOG35052.1"
                     /translation="MKKTLKGWIYLFVTIFFFSTIEVATKPFSSVFNPFQITFLRFFF
                     GGLILLIFPFLTGEIKKFKLTAGRFFLLMGVGALNSLIAMAFLQLAVKYSNASTAAIL
                     VSSNPVFVVLIAWLILGEKITAQKILSLIIGVAGLITIILSRSVGDSFKGIIFGLLSA
                     FSFGFYTVMSKKYIKDIPSTVFAPLSFLFSSIPYYFILKIGGISPFVFEVNLQVILML
                     VYVSLFVTGLAYITFFKALEILDASVSSFSYMLKPVISSIMALAFLGEKPNALKIFGM
                     VLVSMSVLVMILKKGKSKT"
     regulatory      4955..5122
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
BASE COUNT         1721 a          897 c         1074 g         1543 t
ORIGIN      
        1 ttcggggggg tatgatatgc taaaggaaat ttgacgtttt tagaggtgcc cataaataat
       61 attatatgta tgtttaatat tgggtgagat tatgaaaaaa tatatcatac tcggaattac
      121 tcttttttta ataaactgca atattttttc aagtgaacag ttaagagttc ttggggataa
      181 aaactttcct ccatatgaat ttttaaatga agacggtcag attgatgggt ttttagtcga
      241 tctgatgttt tctttatcta atgaaaccgg cagaatgttt gatatacagc tgaaagactg
      301 gactcaggca caggaggaag tgctttcggg aaaagccgat tttctaatgg gaatgaaagt
      361 atctgaaaac cgcctaagaa actttgattt tggtgatccg tacctgaaaa tggatatggt
      421 ggtttttaca cggtatggtt cagctataag gtcgatcaaa gacagtacag agctgatagc
      481 tgccgttgaa gagagtacag cttcacatga ttggcttgtg aaaaacggat ataaaaacat
      541 tttgaaggct tctgactata aaacggccct tgagtatctt ctcaacgaaa gtgccgatgt
      601 cctcgtagtg ggagattaca atgcggcaca gtattttata agtgcgcagg atgcagggaa
      661 aaagctggtg ttgacagacc gcctggactc aaattactat gcttttgccg taaaaaaggg
      721 taataaagaa cttcttaaag agcttaacca agcactttca agaataaaaa aagacggtac
      781 ctttgacaaa atatacaaaa aatggtttgg agaagatctg tataactact ccagaatgaa
      841 ggatatatat aacggcatat ttatggctat atttgctgtt cttttggtga tgattgtgtt
      901 tctgatgata ctttttataa agctgcgcag acgcacaaga gagatccaga cgaaaaatat
      961 tgaacttcag acggcgtaca ggcataccga ggaccttaca cttaagcttc agacacttat
     1021 agatatttct tccaagacca tattcaagaa aggtctgagt gagcagactt tttttgacga
     1081 gcttctgaag gcttctttca aaatagtgcc tgaggcagat tacggaagca tatccttggt
     1141 agatgaggac agatggtgct ttgtttctgc tgtggggcat gatattgaaa agctcaggga
     1201 acttgatctt aaggctgaat atatgataga tgcccgtgat ataaaaatag taaatcatat
     1261 atttgaggaa aacagaaaaa tacttccgaa ggaacttttc gaaaagatca atgaagcttc
     1321 cgtcagggta aaagagtcta tgatagtatc catggagatc gaaaacagac ttgtgggaaa
     1381 tatatctctt gatataggag aaaacaatcc ctatgttttc acagaggaat caaaaaaagc
     1441 gcttcggagc tttgcgaata ttgcagcttc cttcatggcg tacaagaact ttctgtctat
     1501 gaagaacaga gttcaggagg aagtgatatc ggctgtgctg aatatacttg atatgcatga
     1561 tccctatact aaaggtcatt ctaagaatgt tgcaattctc tccagaagta ttgccgctga
     1621 aatgggatat gccgaaaatg aatgcgaaaa aatatactgg gcgggtttgg ttcacgatat
     1681 aggcaaaatc ctcatatcgg tgggtatact gaacaagcct gccaaactaa ctgttgatga
     1741 atatgatgag ataaaaaagc atcctgttta cggatatgaa ttttttaaca ggaatcaagg
     1801 tcttaaagat attgccgaaa ttgttcttta tcatcacgag cgttatgacg gcagaggtta
     1861 tccaaaagga cttcgtgaag gtgagattcc tgtattttca agaataatag ctgtggctga
     1921 tgcttttgat gcaatgacaa acaatcggtc ctaccgaaga gcggtaagca ccgatgaagc
     1981 agttgcagag cttaaaaaag aatctgccaa acagtttgat cctcatattg tagatatagc
     2041 tgtcagaata attcgttctg aagcaggtct caatattttt tcctgaaaac tccggaaagt
     2101 attactattc ctacaaaaat aagaaatgct ccgaatacaa gcgctccggg agcggttgaa
     2161 ctcatactca gcatgaaaaa gaatgcggaa ataatactga ggatacttac cggtatcata
     2221 agagcaggta ttctcatacc aaacagataa agctggaaaa gtccgactgc cggtgccagt
     2281 atgaatacag gccataaaaa agccatatag gcgtagtcag taaatcccag gaaaagaaag
     2341 agcaatccta tagtacataa gatgcctcca ggaacaagca gtcctgcacg taatggtgcg
     2401 ggtctgtgga aaaagctcag ttcaaaggcg atacctggta taaggataaa cagaggccat
     2461 attaaagccc atactttcac ttgcggaaag ataaccccaa gaagaatagt gactccaagg
     2521 aaaataagca gtaaagagat ccaaatagaa ccgttgcgtg agttatacat ttttccctcc
     2581 ctcttttgat aaaaatcaaa aaattagaac tttacaaaat aaaggccaag aacaaaaaat
     2641 gttcccaaga tcaatgaaac taaaaaggtt tttttggagt aattcataaa ctggctgaaa
     2701 gaaaggcttt ttccgccgtg cttttccagc agtgacactc cgacgatgtt ttggacagca
     2761 cctaaggggg tgaagttgct tccgaggttt gcccctactg cgtacgcata ccatagctct
     2821 gccggtgccc catagcctat aagtatttta accacgggtg ctaaaagaag ggttccgggt
     2881 acagcactta aaaaaggtat gacaaaagcg gagagccaca tgaagcaaag catgagaatc
     2941 acaggagcac ctataagtgg tttaaatatc caggctatcg actgagtgac accaatttta
     3001 tccagagcat atgaaatact gaaaagccct gcataaaaga acatcgtatc ccagtctatc
     3061 ctagaggaaa aagatgcaaa atcgtgatta aaaacaaaaa gaaggattag ccctccgaat
     3121 accgctataa gagcagaatt gatctttatg gcagaactga aaataaaacc tgtcagaacc
     3181 aatgcaaaga ctataattga tattaaaaga agcttcttat ttgttatagc tttctccggc
     3241 ttcatatttc ccaaccgttt gagtttttct gagaactctg ctgtctgagc tttcgtatat
     3301 ttttttctga aaatagtgaa tgtggagata aaagtaataa acgctattac cgacattatc
     3361 ttcatgaact gcgtaaaatc aagtttgctt atgcttccca gaacaatatt aagaggactt
     3421 ccgataagcg ttgacatccc gccgatattg tctatgaata tagtaagcat gataaaaggt
     3481 gctgcatcta tctcaagcgt atcggtaata agaaagatta taggagatat gaaaagtatg
     3541 gttatgagat tgtcaagaaa agctgaaaaa agagcggtaa tgagcatgag cgaaaacaaa
     3601 gctgcccaaa actttgcttg cttttataac ccatacagcc gaaaatgtga aaaaaccgct
     3661 ttccttaaga aattctacta ttatcatcat cccgaaaagt atgcccaagg tttcaaagct
     3721 gatgatcttt ccgatgtttt ccaatgtaag ggtatttaca ggattgaaga tataaagtat
     3781 aatagacagt aaaagggtgg tgacggattt tttcaccttc ttggaaaaaa taatgaagta
     3841 gtaggccact atcgcaatta ctatgacaag agcgccgttt aacatttgaa ttcctcctta
     3901 taagatctga aacaattcta tcacggagaa caaaataatc tttttaaaat agattaaaat
     3961 attgtgtatt attacataag taggaggatt accttgaaaa aaactctcaa aggctggata
     4021 tacctttttg taaccatctt ttttttcagc acgatcgaag tagctaccaa accattctct
     4081 tctgttttta atccttttca gattaccttt ctcagatttt ttttcggtgg actgatactt
     4141 ttaattttcc ctttccttac cggtgaaata aaaaaattta aacttactgc aggcagattt
     4201 tttctgctga tgggtgttgg agctttaaac tcattgattg caatggcgtt cctgcagctg
     4261 gcggtaaagt attcaaatgc ttctactgct gccatactgg tgagctcaaa cccggttttt
     4321 gtggttttga ttgcctggtt aatactggga gaaaaaatta cagctcaaaa gattctttca
     4381 cttattatag gtgttgcagg acttatcact ataatacttt ccaggagtgt tggagattct
     4441 ttcaaaggaa tcattttcgg gttgctctct gcattttcat ttggtttcta tacagttatg
     4501 agcaagaagt atataaaaga cattccttca actgtatttg caccgctttc atttcttttt
     4561 tcatcgatac cgtattattt tattcttaaa ataggaggta tcagtccttt tgtcttcgaa
     4621 gtgaaccttc aggttatact tatgcttgtc tatgtgagtc tttttgttac aggtctggct
     4681 tacataacct ttttcaaggc gcttgagatc cttgatgcct cggtcagctc tttttcatac
     4741 atgctcaagc ctgttatttc aagtatcatg gcactggctt ttcttggtga aaagccaaat
     4801 gcgttgaaga tattcggtat ggttcttgtc agtatgagcg tgcttgttat gattttgaaa
     4861 aagggcaaaa gtaaaacata aaaaatattt tttgtaattt aattttaaac aaaaagcaac
     4921 atttttaagt taaaatgata taacaaagtt aataaatgat tacggaggag cggcacagaa
     4981 tagtaccctg cacacaaacg agtcttatta tgctggggaa aggcaagacc gccgaagggt
     5041 ataaaaaaga ctctttttat acttctgggt atgcaggaaa tacctgtata actgtcactt
     5101 atagtggaga gccgtcaaat ggatgattta atgcaaattt gccaaatcat ttatggaaat
     5161 ctctccctcg aaagagggtt tttgattttt atatgataat tctcctcata aaggagaatt
     5221 ttttaattta atggg
//
LOCUS       DAOSHR010000143         5186 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_14229_length_5186_cov_5.374391, whole genome shotgun sequence.
ACCESSION   DAOSHR010000143 DAOSHR010000000
VERSION     DAOSHR010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 5186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5186
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14229_length_5186_cov_5.374391"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1923)
                     /locus_tag="PLU28_08075"
     CDS             complement(<1..1923)
                     /locus_tag="PLU28_08075"
                     /inference="COORDINATES: protein motif:HMM:NF028500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOG35053.1"
                     /translation="MNEETLLNSFEEVLILKEKNLKKWEDIFPTFYSLVRTGISQLSS
                     NIDFMKDLKSVMEYAKPLAELPAERKAKRFSSLKEVVSEMKEKYLIDNTIQPPEKSLS
                     LYTDVKFLKGVGEKRAGLLNSLEIRNIYDLFYFVPREYEDRRNVKTIFNCSNNEPCVI
                     VAKVLQLEESKVNNSLKILNVVVSDDTGILTVSFFNQEYLKTMMTQDSWIAFFGKTEE
                     FYGRKIMKSPDFHILADKNSYIKMIHPVYPLTKGLSQQIAKNILMEALKYLSLCEEFI
                     PESIIEKYNILSITSRIKGVHFPKTFYHRDRALFSMKVQEAVTFETAVLLSKNNAKTG
                     FVGTAKVFSGELSERFVKSLPFSLTSAQKKAYSDIQNDLRDKLPMNRLLQGDVGSGKT
                     VVAQMAMLDTVQAGFQVAMMVPTSVLARQHYAKLTEEFSGLDINVSLLLGDTKDSEKN
                     EIKKNIETGQVDILIGTHALIQENVSFKNLGLAVIDEQHRFGVNQRLSLINKGISPDF
                     LVMTATPIPRTLALTVYGELDISTIDQMPKGRKNVITRTLDDSKRKEMYSFVREELSN
                     NNKVFFVYPLIEESEKVDLKSAQEMYSSELKNEFSDYGVGLIHGKMSSFEKNEIMEKF
                     SKGSINVLVSTTVIEVG"
     gene            complement(1926..2816)
                     /locus_tag="PLU28_08080"
     CDS             complement(1926..2816)
                     /locus_tag="PLU28_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOG35054.1"
                     /translation="MKRTAIIVDSGCDPSPVFIEKYKLNFMGMKIVIDEKDYTDGEDI
                     KKDDFYKIITKAKEFHTAHPSVGYVVKKYEDIVAQKYDEIIDIHFSSKMSGLINTCLL
                     AKNMVPAANVKIIDTESVSIESFLIAEKVVELIREKGWSYEQVMEVLPEIKASAFMQF
                     NVTTLNYLVKNGRIGKAAGLAGTLLNIKPILGVNDGYIAPIDKVRGMSKVYSVIVENA
                     INFLKDRPYNSKVFITYGGENNIEHMKETYNLFLEKQKEINLPSHRLIESRISPTIIC
                     HSGPEVFGFAVYGEKEPIGL"
     gene            complement(2820..3029)
                     /locus_tag="PLU28_08085"
     CDS             complement(2820..3029)
                     /locus_tag="PLU28_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35055.1"
                     /translation="MGLFIITSYVYYKITSFETGENDSLLEFYTYQNNIADVSKYLKA
                     GVLEQIIINDLIIKLENGSIIIQRR"
     gene            complement(3068..>5186)
                     /locus_tag="PLU28_08090"
     CDS             complement(3068..>5186)
                     /locus_tag="PLU28_08090"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOG35056.1"
                     /translation="LRTTIGNPNFVSDYLAQLLPISVYFILKKDSSKLLKVYNFINIF
                     LFYWVILLTQTRSIFLGFFVGLVFAFICLIINLRHIDDKKYFIGKNFLLKVFSIIAIL
                     VFLFAMFSMPTFFNKEGEIIPSNRFNAASTVSSWDERSLSWLASVEQFLDRDNPQNLF
                     IGGGIGTYQIYAVKYMSIVQNKNPERFLYAWNNFKRTHNDYLQVLGETGLFGFISIAG
                     LLISLLFMYFRSLSKLRDTNKLLLMCMFGWTAVIIALHSFTEFPLHLQPNLALSLFIL
                     SAAVSPQFMSLKTYKIKKINVLLSIVILFGIVSVIFKINSNLSEILFKYGNTEYAKLD
                     YYYDAAQNKIPQAIEEVNTNISQLKKKLQSSVPGSSDFIKTSNELKTAQAQVEKLEAM
                     KSEYLKNSYAAYSSAIDYFNKSLDFNPNFGKSAFYLAQMLTKSPFRYQDLTYDTLNTA
                     FELSTREYKHLVPYFKGNVSLMPFSTSNIRSTVKDLYGKSDSQEYKVILVSIQSIYDE
                     IDMLKTAFISFNEKNSYKLIPKLYFEVIRRYELLKGYCDQSTTEIIENMEKADLNEFM
                     HWYDKAIEILPGGWNRFPEWEQTYSEYLYFLTHMTGIFPQEVIMPKILEVIEKDGKAN
                     YYMAKKVRGIPDSSLTVLEEFLTSIQNSENKSSFIDGIISNYGQVYDFYKRAMENGDS
                     KYLSYKDRVDSFLSTYEFFLRRK"
BASE COUNT         1593 a          971 c          910 g         1712 t
ORIGIN      
        1 tccaacttca atcaccgtag tgcttaccag tacgtttata cttcctttgg aaaacttttc
       61 catgatctca tttttttcaa aagatgacat ttttccatga attaagccaa cgccataatc
      121 agaaaactcg ttttttaatt ccgatgaata catctcctgt gcagatttaa gatcaacctt
      181 ttctgactct tcaatcaaag gatagacaaa aaaaacttta ttattattgc tgagttcttc
      241 ccggacaaaa gagtacatct ctttacgttt tgaatcatca agagttcttg taattacatt
      301 tttgcggccc tttggcatct gatctatcgt gcttatgtca agctcgccat aaacagtaag
      361 tgcaagagtc ctaggaatcg gcgttgctgt cataaccaga aaatccggag atatgccttt
      421 gtttatgaga gataaacgtt ggtttacacc aaaacggtgt tgttcatcaa ttacagctag
      481 cccaagattc ttaaacgata cattttcctg gataagggca tgtgttccga taagtatatc
      541 aacttggccg gtttctatat ttttctttat ttcatttttt tcactgtctt ttgtatctcc
      601 aagcaaaagt gaaacattta tatcaagtcc gctgaactct tctgtaagct ttgcatagtg
      661 ctgacgtgcc agaactgatg tggggaccat cattgccact tgaaaaccgg cttgtactgt
      721 atcaagcatt gccatttgag ctacaacggt ttttcctgat ccgacatctc cctgaaggag
      781 tctgttcatg ggaagcttat ctctgagatc attctgtata tctgagtagg cttttttttg
      841 agctgatgta agagaaaacg gaagtgattt tacaaatctt tcactgagtt caccagaaaa
      901 aacttttgca gttccgacaa accctgtttt agcgttgttt ttggataaaa gtacggctgt
      961 ttcaaaagta acagcctcct gaactttcat cgagaaaagc gctctgtctc tatgataaaa
     1021 agttttagga aagtgaacac cttttatcct ggaagtaata gataaaatat tgtatttttc
     1081 gattatactt tccggaatga actcttcaca taaggaaagg tactttaaag cctccataag
     1141 aatattcttg gctatctgtt gtgaaagtcc cttggttaaa ggatatacag gatgaatcat
     1201 ttttatataa gagttcttat cagccagaat atgaaagtca ggagatttca ttattttacg
     1261 gccatagaac tcctctgtct taccaaaaaa agcaatccat gaatcttggg tcatcatggt
     1321 ctttagatac tcctgattga aaaaagaaac agtaagaatg cctgtgtcat cagaaacgac
     1381 aacgttaagt atttttagac tgttgttaac cttggactct tccagttgaa gaactttggc
     1441 tactataacg catggctcat tgtttgaaca gttgaagatt gtttttacat tacgcctgtc
     1501 ttcatactca cgcggaacaa aataaaaaag gtcgtatata tttcttatct caaggctgtt
     1561 cagcagacct gcccgtttct ctcctactcc tttgaggaat ttaacgtcag tatataagga
     1621 aaggcttttc tcaggaggct gtatagtatt gtcgataaga tacttttctt tcatctccga
     1681 aacaacttcc tttaatgaag aaaacctttt agcttttctt tcagctggaa gctcagccaa
     1741 tggtttagca tattccatga ctgattttag atccttcatg aaatctatat ttgaagataa
     1801 ttgagaaata cctgttctga ccaatgaata aaaagtggga aatatatctt cccacttttt
     1861 cagattcttc tcttttaaaa tcagcacttc ttcaaatgaa ttcagtaatg tttcttcatt
     1921 catgcttata accctatagg ctccttttca ccgtatacgg caaaaccgaa tacttccggt
     1981 ccgctgtggc atattattgt gggcgatatt ctgctttcaa taagtctgtg ggaaggaaga
     2041 ttaatctctt tctgcttttc aagaaatagg ttgtatgttt ccttcatatg ctctatatta
     2101 ttttctccac cgtaagttat aaagactttg ctgttatatg gcctgtcttt aagaaagttt
     2161 atagcgtttt ctactataac cgaataaacc tttgacatgc ctcttacttt gtctatagga
     2221 gcaatgtaac cgtcattgac accaagaata ggcttgatat tgagtaaagt cccagcaagc
     2281 ccggcagcct ttcctatacg tccgtttttc acaagataat tcagtgttgt aacgttaaac
     2341 tgcatgaaag ccgaagcttt tatttcggga agaacctcca tgacctgttc ataagaccag
     2401 cccttttctc ttatgagctc tacgaccttt tctgcgatca aaaaagattc aatggaaacg
     2461 gactcggtat caataatctt tacatttgct gcaggaacca tattttttgc cagcagacat
     2521 gtatttataa gaccggacat ttttgaagaa aagtgtatat ctattatttc atcgtatttc
     2581 tgagcgacaa tatcttcata cttctttaca acgtagccga ccgaaggatg tgcggtgtga
     2641 aattctttgg ctttggttat tattttataa aagtcatctt ttttaatatc ttcaccgtct
     2701 gtataatctt tttcgtcaat gactattttc attcccataa aattcaattt gtacttttca
     2761 ataaaaaccg gactcgggtc acatccgctg tcgacaataa tagccgtcct cttcatctct
     2821 tacctccttt gaataataat actcccgttt tcaagtttaa ttatcagatc atttattata
     2881 atctgctcaa gaactcctgc tttaagatac tttgaaacat cggctatatt gttctggtaa
     2941 gtataaaact caagcaaaga atcgttttcc cctgtttcaa aagaggtaat tttataatat
     3001 acgtaggaag ttataataaa aagtcccaaa gaaaccacaa gcaagaacga aagaacctta
     3061 taaattttta ctttctcctg agaaaaaact cataagttga taaaaacgaa tcaactctgt
     3121 ctttatatga gagatacttg gaatcgccat tttccattgc tcgtttatag aaatcgtata
     3181 cctggccgta attggatatt ataccatcta taaatgaact tttattctct gaattctgaa
     3241 ttgaagttaa aaattcttca aggacagtca gagaactatc cggtatacct ctaacctttt
     3301 ttgccatgta gtagttggct tttccatcct tttcaattac ctcaagaatt ttaggcataa
     3361 taacttcttg cgggaatatt cctgtcatat gggtaagaaa gtaaagatac tcactgtagg
     3421 tctgttccca ttcggggaat ctgttccatc ctccgggaag gatttctatg gctttatcat
     3481 accaatgcat aaactcattc agatctgctt tttccatatt ttctattatt tccgtagtac
     3541 tctggtcaca gtagcctttg agaagctcat aacggcggat cacttcaaaa tatagcttag
     3601 gtataagctt atacgagttt ttctcgttga acgatataaa agcagtttta agcatatcta
     3661 tctcatcata aatgctttgt atggatacaa gaattacctt atactcctgc gagtctgatt
     3721 ttccgtagag atcctttaca gtacttctga tatttgaagt tgaaaacggc ataagagata
     3781 catttccttt aaaataagga accagatgct tatactcacg tgttgaaagt tcaaaagcag
     3841 tattgagcgt atcgtaagta aggtcttggt acctgaaagg agactttgtg agcatctgag
     3901 ccaaatagaa ggcagatttt ccaaaattag ggttaaaatc cagcgactta ttaaaataat
     3961 ctatagcaga gctgtaagca gcatatgagt ttttaaggta ctcagacttc atggcttcaa
     4021 gtttttctac ctgcgcctgg gctgttttaa gttcattcga agtctttatg aaatcagacg
     4081 aacctggcac tgaactctgt aacttctttt ttaactgtga aatattggta ttaacctctt
     4141 ctatagcctg gggaatttta ttttgagcgg catcataata ataatcaagc ttggcgtatt
     4201 cggtatttcc gtatttaaaa agaatctcgg agaggttcga atttatttta aagataaccg
     4261 atactatgcc aaaaagtata acaattgata acagaacatt tatttttttg attttgtaag
     4321 tttttaaact catgaactga ggcgaaacgg cagccgaaag tataaacaaa gacagagcca
     4381 gattaggctg cagatgaagc ggaaactcgg taaaggaatg aagggctatt attactgccg
     4441 tccatccaaa catgcacata agaagaagtt tatttgtgtc tctgagcttt gaaagtgatc
     4501 tgaaatacat gaacaacaga gatatgagta atcccgcaat gcttataaat ccgaaaagac
     4561 cggtttcacc caacacctga agatagtcgt tgtgggttct tttgaagtta ttccatgcgt
     4621 agaggaatct ttcgggattt ttattctgta ctatactcat atactttact gcatatatct
     4681 gatatgtacc tatgcctcct ccgataaaaa ggttctgtgg attgtccctg tcaaggaact
     4741 gttctacgga ggcaagccat gaaaggcttc tttcgtccca ggaagaaacg gttgaagcag
     4801 cattgaacct gttcgaaggt attatctctc cttctttgtt aaaaaaggtc ggcatgctaa
     4861 acatagcaaa aaggaaaacc agtatagcaa taattgaaaa aacctttaaa agaaagtttt
     4921 taccaataaa gtatttttta tcgtctatat gtcttaaatt aataattaag cagataaaag
     4981 caaacactag accgacaaaa aaacctaaaa atatagagcg tgtctgagta agaagaatta
     5041 cccagtaaaa aaggaatatg ttaataaaat tatatacttt cagaagcttg ctggaatctt
     5101 ttttcagaat gaagtataca gaaatcggta gaagctgggc aagataatca gaaacgaagt
     5161 tcgggttgcc tatggttgtg cgcaga
//
LOCUS       DAOSHR010000144         5178 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_14257_length_5178_cov_4.996682, whole genome shotgun sequence.
ACCESSION   DAOSHR010000144 DAOSHR010000000
VERSION     DAOSHR010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 5178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5178
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14257_length_5178_cov_4.996682"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="PLU28_08095"
     CDS             complement(<1..310)
                     /locus_tag="PLU28_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HOG35057.1"
                     /translation="MDEYKRIKGTQDIYGLEIDYWNQVEQIAKSILEKYAFKELRTPV
                     IEPTELFNRGIGNETDIVKKEMYTFEDKGGRSITLRPEGTAPAVRAYIENSMINSGLP
                     I"
     gene            complement(427..801)
                     /locus_tag="PLU28_08100"
     CDS             complement(427..801)
                     /locus_tag="PLU28_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOG35058.1"
                     /translation="MVKGIESGETVKAKVVDIKKFGAFVKLDNGEEGFIHISKISKNY
                     VKNISDFLQVGQEIEGRIIGTTKDGKYELSLKEGSVPEDTSKETNEEFEKKLSQFMRD
                     SEKKISEYNKHLEKKRGVSKRR"
     gene            complement(1019..1234)
                     /gene="rpmE"
                     /locus_tag="PLU28_08105"
     CDS             complement(1019..1234)
                     /gene="rpmE"
                     /locus_tag="PLU28_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HOG35059.1"
                     /translation="MKKGIHPEMKFITVKCACGAEHKIYSTVDTFRLDVCSKCHPFFK
                     GEIGSQILDTEGRVKKFNNRYKNFTQA"
     gene            1434..3263
                     /gene="lepA"
                     /locus_tag="PLU28_08110"
     CDS             1434..3263
                     /gene="lepA"
                     /locus_tag="PLU28_08110"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HOG35060.1"
                     /translation="MYNPELIRNISIIAHIDHGKTTLVDRILEKTKTIELRKMKDQFM
                     DSMDIESERGITIKAKPVKIFYDSADGNRYEINIIDTPGHVDFNYEVSRSLAACEGAV
                     LLVDASQGVEAQTVTNTYLAIENDLEIIPILNKIDLPNANIEESLLELDDLIGIEQKD
                     VLLVSARTGKGIDDLLESIVKKIPSPSSKGSSSEKLKALIFDAQYDKYKGIIVYTRVF
                     SGSMKKGDKVRFMVSEMDYEILEVGLFHPQMEETDTLCAGEVGYIVAGIKDLQEAKVG
                     DTITNTQNPIDKPLHGYKEVKPNVFAGLYPGLPEYFEELRKSIDKLNLNDTSLVYSIE
                     NSPAMGFGFRCGFLGLLHMDVVRERLEREFEISVILTAPSVVYRAKLKNGETIEINNP
                     AEFPLQDRIDEVYEPYAKLDIITPPDYMGSLINLAQIEKRGNFISVSNAGKNRVVLHF
                     EIPLAELIFDFFDKMKAISRGYASMDYEILEYRKSDLVKITIFVNKEPIDALSFIEHR
                     SKAYDTARKVVDKLPDLIPRHQFEIPIQAYADGKIIARSTIKAFRKDVLAKCYGGDVT
                     RKMKLLEKQKEGKKRMRAIGTVDIPQEAFLALLRIGDEEDEKK"
     gene            3331..3696
                     /locus_tag="PLU28_08115"
     CDS             3331..3696
                     /locus_tag="PLU28_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35061.1"
                     /translation="MLEVSAKKGRFESLKKIGNGVSAFFKFLSKPAVLWITSIAVLLV
                     FSLVVDIQWLIIIPVIVATFSKQWLAAMLTSFVRGTGIFGMISVSMLWITFGLLVFFI
                     YGIYTFFFDSKKFLKIKRK"
     gene            complement(3722..>5178)
                     /locus_tag="PLU28_08120"
     CDS             complement(3722..>5178)
                     /locus_tag="PLU28_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOG35062.1"
                     /translation="LTKMQYTRKEDESSGGVFRFKGEVLEVFPPYEDYGIRIEFFDEE
                     IQSIKTFDLLNRKTIEIIDKVTLYPAKEYVTTEEKIRTAAEKIKADLADRIENFKSQG
                     KLLEAQRIEQRTSQDIEFLEVLGHCKGIENYSRYFDGRLPGDPAWSLLDYFEKDFITF
                     IDESHIAIPQIRAMWRGDYARKKNLVDYGFRLPSAFDNRPLKFEEFLQKTNQIIMVSA
                     TPGPFEYEVSQNIAEQIIRPTGLTDPLVEVKATEGQIDSFVDELSHVIKRGERSLAVV
                     LTKKDAEMLSEHLNLIGIKATYLHSELDTMERNEVVRKLRTGEVNVVVGINLLREGLD
                     LPEVSLIAIMDADREGFLRSETTLIQTIGRAARNVNGKVLLYADKITPAMEKAISETN
                     RRRAKQIEFNIQNNITPKTIIKEIPQEDIFAPFKEEMAPDKYILEAAKGTNAEDYMAL
                     LEEEMLKAASELRYEDAAKLRDEMNKARKKYNIK"
BASE COUNT         1484 a          975 c          968 g         1751 t
ORIGIN      
        1 ttattggaag tccggagttt atcatagaat tttcaatata tgcccttaca gcaggtgctg
       61 ttccctccgg gcgcaaggta attgaccgcc cgcctttatc ttcaaaagtg tacatttctt
      121 tttttactat gtctgtttcg tttcctattc ctctgttaaa aagctctgta ggttcaatta
      181 caggtgtacg aagttcttta aaggcatatt tttcaagaat gcttttagca atttgttcaa
      241 cctgattcca ataatctatc tccagaccgt atatatcctg ggtacctttt attcttttat
      301 actcgtccac attattcgtt caagagttac ttgaacttca cctcctaaaa aattatatca
      361 gtttctatta taacacaaaa gggagccgcc ggctccctgg taagtctatg tttgaattat
      421 cttttattat cttcttttcg aaacaccacg ttttttctca agatgcttat tgtactccga
      481 aatctttttt tcactgtctc tcataaactg actgagtttc ttttcaaact cctcattcgt
      541 ttctttcgaa gtatcctcag gtactgatcc ctccttcaag gatagttcgt acttaccgtc
      601 tttggttgtg ccaattattc tgccttcaat ctcctgacca acctgaagaa aatcagagat
      661 gttctttaca tagttcttgg aaatcttaga aatgtgtatg aatccttctt ctccgttgtc
      721 gagtttaaca aatgccccaa actttttaat atcaactact tttgccttta ctgtttctcc
      781 tgattcgata ccctttacca ctatgattcc ctgcccttct ttttaagatt tttcacgata
      841 accttgcgaa gtcatgatac gtttaaacgg cccgggtcca ggctatggtt tgtcacctcc
      901 aagtgttttt ttatttgata ttgccagaaa ttccgaaaga actgcaaggc tggcaatatc
      961 taagcttttc aatttttata aaattgaagc caaaaaaggg agataaaatc tcccttgatt
     1021 atgcctgagt aaaattctta tatctgttgt tgaacttctt tactcttcct tcagtgtcca
     1081 aaatctgaga acctatctca cctttgaaga aaggatggca cttggaacaa acatcaagcc
     1141 tgaaagtatc aacagtggaa tatatcttat gctctgctcc acaggcacat tttacagtga
     1201 taaacttcat ctcaggatga atcccttttt tcattctttt tccacctctt ggttattttt
     1261 gatttccgtg ttattataac acaatgattt ttcttgtgtc aagatgcaaa caaaagctta
     1321 atgtcaactt aacgttgaaa atataagcat tacctctcag aatattctat ttttaattat
     1381 tttttgctat aattattctg tcataattct ggttgcactt ggaggtgtct gttttgtata
     1441 atccggaact tataaggaac atttctatta tagcacatat tgaccatggg aaaacaactt
     1501 tggtggatag aattttggaa aaaactaaaa caattgaatt gaggaagatg aaagatcagt
     1561 ttatggattc catggatatt gaaagtgagc gtggaattac aataaaagcc aaacctgtaa
     1621 agatatttta tgattctgcc gacggaaata ggtatgagat aaatattatt gatactcccg
     1681 gtcatgtcga ttttaattac gaagtttcaa gaagccttgc tgcgtgtgaa ggtgctgtgc
     1741 ttttagttga tgcatcccag ggggttgagg ctcagacggt taccaataca tacttagcta
     1801 ttgaaaacga tcttgagatt attcccatat taaataaaat tgatttgcca aatgccaata
     1861 ttgaggagtc tcttttggaa cttgatgatc ttataggtat agagcagaag gatgtgcttc
     1921 ttgtaagtgc aagaaccggt aaaggaattg atgatcttct cgaatctata gtaaaaaaga
     1981 taccttctcc ttcgtccaaa ggtagttctt cggaaaagct taaagctctt atttttgatg
     2041 cacagtatga caagtataag ggaattatag tttatacgag agttttttca ggatccatga
     2101 agaagggcga taaagtaagg ttcatggtat cagaaatgga ttatgaaata cttgaagtag
     2161 gtctttttca tccacagatg gaagaaacgg atacactttg tgcaggagag gtagggtata
     2221 ttgttgctgg tataaaagat ctgcaggagg ccaaagtagg agacaccatt acaaatactc
     2281 agaatcctat tgataagccc cttcatggct ataaggaagt taagccaaac gtatttgcgg
     2341 gtctttaccc gggactaccc gaatattttg aggagttaag aaaatcaata gataagctca
     2401 accttaacga tacctccctt gtttattcca tcgaaaactc gcctgcaatg gggttcggtt
     2461 tcaggtgtgg ttttttgggt cttcttcata tggatgtagt gagggaacgt cttgaaagag
     2521 agtttgaaat aagtgttatt cttaccgctc caagtgtagt ttacagggct aagcttaaga
     2581 atggtgagac aattgaaata aacaatcctg ctgaatttcc tttacaagat aggatagatg
     2641 aggtttatga gccatatgca aaattagata taattactcc gcctgattac atgggaagtc
     2701 ttataaatct tgcacagatt gaaaaaagag gtaatttcat ctcagtttcc aatgccggaa
     2761 aaaatagagt ggttctccat tttgaaattc ctctggccga actgatattt gatttttttg
     2821 ataaaatgaa agccataagt cggggttatg catccatgga ctatgaaata cttgagtaca
     2881 gaaagtctga tttggtaaaa ataacgatat ttgtgaacaa agagccaata gatgcccttt
     2941 cttttataga gcacagatca aaggcctacg atactgcccg aaaggtagtt gacaagcttc
     3001 cggatcttat tcccagacat cagtttgaaa tacctattca ggcatatgca gacggtaaga
     3061 ttatagcccg ttcaaccata aaagctttca gaaaagatgt acttgcaaaa tgctacggag
     3121 gagatgtgac ccgaaagatg aaactgcttg aaaagcagaa ggaaggaaag aagagaatga
     3181 gagccatagg aaccgtagat atccctcagg aggctttcct tgccctattg agaattggtg
     3241 atgaagaaga tgaaaaaaaa tagtattgtt gcaaaagagt aaaatttaag gtataataat
     3301 tatagtagaa acataataac ggaggtgggg atgttggaag tttcagcaaa aaaaggcaga
     3361 tttgagtcct tgaaaaaaat cggcaacggt gtttccgctt ttttcaagtt tctttctaaa
     3421 ccagcagtct tatggataac atcaatagct gttctgcttg ttttcagtct tgtggttgat
     3481 attcagtggt taataatcat tcccgtgata gttgccactt tcagcaaaca gtggctggct
     3541 gccatgctta catcttttgt aaggggaaca gggattttcg ggatgataag cgtgagtatg
     3601 ctttggatta ctttcggtct tttagtattt tttatatacg gtatctatac attctttttt
     3661 gattctaaaa aatttcttaa aataaaaaga aagtaaaata aactcccata acgatgggag
     3721 tttattttat gttatatttt tttcgggctt tattcatctc atcgcgaagc ttggcagcat
     3781 cttcatatcg cagctcacta gctgctttta acatttcttc ttccagcaga gccatataat
     3841 cttccgcatt tgtgcctttt gcggcttcaa gtatatactt gtccggagcc atttcttctt
     3901 tgaacggagc aaagatatct tcctgaggaa tttcttttat tattgttttc ggagtgatgt
     3961 tgttctgaat attaaactca atctgtttcg cacgccttct gttggtttcg cttatggctt
     4021 tttccattgc cggagtaatt ttatcggcat atagcagtac ttttccgttt acatttcttg
     4081 ccgctcttcc tatagtctgt ataagtgtgg tttcagacct caaaaatccc tctctgtcag
     4141 catccattat agctataaga gatacttccg gaagatcaag accttctctg agaaggttta
     4201 ttccaacaac tacgttgact tctcctgttc tgagttttct gactacctca ttcctttcca
     4261 tcgtatcaag ctcggaatgc aggtatgttg cttttatacc gattaggtta agatgctcgg
     4321 agagcatctc agcatctttt tttgtcagta ctactgccag agatctttcc cctcgtttaa
     4381 tcacatgact cagctcatcc acaaaactgt caatctgtcc ctcagtagct tttacttcga
     4441 caagagggtc agtaagacct gtaggtctga taatctgttc tgcaatattt tgagatactt
     4501 catattcaaa aggaccggga gtagcagata ccataataat ttgattggtt ttttgtagga
     4561 actcttcaaa tttaagagga cggttatcaa aagctgacgg aagtctgaat ccgtaatcga
     4621 caagattttt ctttcttgca taatcgcctc tccacatagc tcttatctgc ggaatagcta
     4681 tatgcgactc atcaataaaa gttatgaaat ccttctcaaa gtaatcaagg agtgaccatg
     4741 caggatcacc gggaagtctt ccgtcaaagt atctggaata gttttcgatt cctttgcagt
     4801 gccccaggac ctcaagaaat tctatatcct gagatgttct ttgttctatc ctctgtgcct
     4861 ccagaagctt accctgactt ttgaaatttt ctattctgtc tgcaagatct gcttttattt
     4921 tttctgccgc tgtccgtatt ttttcttctg tggtgacgta ttctttggcc ggatagagtg
     4981 tcactttgtc aataatttca atagttttgc ggttcaataa atcaaaagtt tttatactct
     5041 gaatctcttc atcaaaaaac tcaattcgta ttccgtagtc ttcataagga gggaaaactt
     5101 ctaagacctc ccccttaaat ctgaaaaccc ctccggagga ctcatcctct tttcttgtgt
     5161 actgcatttt agtaagtt
//
LOCUS       DAOSHR010000145         5094 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_14582_length_5094_cov_4.668982, whole genome shotgun sequence.
ACCESSION   DAOSHR010000145 DAOSHR010000000
VERSION     DAOSHR010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 5094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5094
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14582_length_5094_cov_4.668982"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1253)
                     /locus_tag="PLU28_08125"
     CDS             complement(<1..1253)
                     /locus_tag="PLU28_08125"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOG35063.1"
                     /translation="MKKRSIIFLLLITLLCVSVLSAKTIELKFWTHEDPNRKEIEMRY
                     IQEFQKMYPDVVIKRVEYSSTKIQELILTAFAANEGPDIFNMSIEDEYAYIVNGRVAP
                     VNPKAAGYTSLKAIYAEYMQGMLDPVTFEGNLYGLPLEITNWCIFLNKKVFRDAGLNP
                     EKDYPKTWEDVVALSEKIAIRKGDIVQRRGFDFRYPYYLVEMVPLVEQLGGKLVSDDG
                     KTAIINDEAWIKFLKFMADFGPKGKNLGSPTYQAARKLFNNDNNDIAMCSTGLYQIAR
                     IKADNQKFFDSNEWMVIPFPQFKNAVRNVPGKYYGHYYMVNAQKPKETQEMAWKFIGY
                     MLSHAEEYLEKVAIIQPKLSLINSNLFKSYPYSNVFLSDLQKAEVVFYQANSAKIQKH
                     IEEAVQSVMFGNKTPEEALKILKQKA"
     assembly_gap    1540..1639
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1640..2790
                     /locus_tag="PLU28_08130"
     CDS             <1640..2790
                     /locus_tag="PLU28_08130"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOG35064.1"
                     /translation="VFVLKNLYKIKVKDDSSVSEKTLCILCADVQEIFRLIPANMKVS
                     SVSLEGIYPQNKRIPLKSMKKIVQSIYLMMNSGINLYDCLFMIAVGDEYDKFTRGVVC
                     RSYFHIVRGDDYKKCFELAEYDGYFSLGMGICETRDMFVSNLGSFLSYYTEKEKVQSE
                     LKKSLLYPFTVLLSLTVLLFFLRFYIIPSFSSLLNIEINFTVSSVLFVCFFLLLILIS
                     VSYVFARKNDSYLCKIPFLGEFYKTIVLYRFVRDINLLISSGLTLYEAMDKTVESIDS
                     VLAKKAFLSPGLDIRSGLDVKEAFCKVKDFKEISLAFALSGKVGGYRQVLDFLQKDCQ
                     SRIKESSEKILKMIEPVLIILISVLIMSIAYEVYSKVFIGNINFSVGE"
     gene            2798..3133
                     /locus_tag="PLU28_08135"
     CDS             2798..3133
                     /locus_tag="PLU28_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35065.1"
                     /translation="MKSVEFRKGFLMLELSIYLIAEVIMFSSLCAVSILPLYRYFERL
                     SDQVLAVKTINRMRTVSFISRKADIYASKTSVIAGFRNNYFTFKINQYSYRELTFDDG
                     YIKSRRDYS"
     gene            3191..3637
                     /locus_tag="PLU28_08140"
     CDS             3191..3637
                     /locus_tag="PLU28_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35066.1"
                     /translation="MKKTIFLKYNGKKIGVGHMDCYIQSYQTEQHLLKDLFKKGISKR
                     VGFFEMGSWDEKYYLIYDKSHWLKKRLSPIQDIFIPQQLLAVDTLLKMPSGEYVIKFP
                     CCSYFFERNKYYIKNGIVCDRHEYDVSFSVQDINKAVLEYAKKGGL"
     gene            3634..4032
                     /locus_tag="PLU28_08145"
     CDS             3634..4032
                     /locus_tag="PLU28_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35067.1"
                     /translation="MIFSVKSLSRKPFLLLLDLLIIVLAVMTAYSFFGGVSSHKKVAD
                     EKKYEKSKYEKYLKSIREEKNEISEFFRVLEKYDARLIEFTFVGGRIESTVMLELSEG
                     QEIITKYAVKKISELKTGEKSIVFLSIFTE"
     gene            4042..4638
                     /locus_tag="PLU28_08150"
     CDS             4042..4638
                     /locus_tag="PLU28_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35068.1"
                     /translation="MRIRALVALVLLAFFLILSMLSKEEPYEEQVFILYSIGSGSIDR
                     KFSNKELKKFMSAVLADDSKVDSIKLLRENSSVNNISTDFTLQVKQSLPSEGEFAYKT
                     LICAESAEIAESIFDGKVFSLEYLWSMKDSFKSFMPDCIRFAGVRVKNGVKSAYIYFN
                     DELLNVCEGEVIGDRQIMGIFDDGILVLTCGKEIEVIL"
     gene            4635..>5094
                     /locus_tag="PLU28_08155"
     CDS             4635..>5094
                     /locus_tag="PLU28_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35069.1"
                     /translation="MKKIFLVIGLLLLIGLSFTMSIDYDKKTNANVILFDFQKEFTVV
                     SNSELTVHKILFGEELFIPQSYKIEKGPLRSVEILSNMLEIRTLMPVKPLVDGKNRLI
                     FNGRGALNFEKLFLKEIKLGDLLNLFSQELKLNWIRMEPFPQEKLSLWSDE"
BASE COUNT         1535 a          815 c         1035 g         1609 t
ORIGIN      
        1 gctttttgtt taagtatctt caaagcttct tcaggtgtct tgtttccgaa cattaccgac
       61 tgtacggctt cctcaatatg tttctgtatt ttggctgagt tggcctggta gaaaacaacc
      121 tcggccttct gcaggtcaga caggaatacg ttagagtaag gatagctttt gaaaagattt
      181 gagttaatta gtgaaagctt aggctgtatt atggcaactt tctcaagata ctcttcggca
      241 tggctgagca tatatcctat gaacttccaa gccatttcct gtgtttcctt aggtttctgt
      301 gcgttaacca tgtaatagtg gccgtaatac tttccaggaa cgtttcttac tgcattttta
      361 aactgtggaa atgggataac catccattca ttgctgtcaa agaacttctg attgtcagct
      421 tttattcttg cgatctgata taatccggtt gaacacattg caatatcatt gttgtcgtta
      481 ttgaaaagtt ttcttgcagc ctggtatgta ggcgaaccaa gatttttgcc ttttggaccg
      541 aaatctgcca tgaacttaag gaattttatc catgcttcat catttattat tgcagtttta
      601 ccgtcatcgc tcacgagctt tccgccaagc tgttctacaa gcggaaccat ctcgaccaga
      661 tagtatgggt acctaaagtc aaatcctctt ctctgtacta tatcgccttt tcttatggct
      721 atcttttccg acaatgcaac aacgtcttcc caagttttag gatagtcttt ttccggattc
      781 aaacctgcat ctctgaaaac ttttttgttt aggaatatac accagttggt tatctcaagt
      841 ggtagaccgt aaaggtttcc ttcgaacgtt acaggatcaa gcatgccctg catgtactcg
      901 gcgtaaatgg cttttaaact tgtatatccg gcggccttgg gattaacagg cgctactctt
      961 ccgttaacaa tataagcata ctcatcttcg atagacatat tgaagatatc cggtccttca
     1021 ttggctgcaa aagcagtaag tataagctcc tgaatcttgg ttgatgagta ctctactctc
     1081 tttatgacaa catccggata cattttctga aattcctgga tgtaacgcat ttcgatctct
     1141 tttctgttag gatcctcatg tgtccagaac ttaagctcta tggtctttgc actgagaaca
     1201 gagacgcata acaacgtaat aagaagtaaa aaaataatgc tgcgtttttt catgtaagta
     1261 cctcccgtaa tagaatattt tcaagataac aataaataag caatccaatt ataactcaaa
     1321 gatatctctg acattaagtt cattctgcgg tgtatattta agattaaaga gtatgccatg
     1381 cagtaatccc ttaattttct taaaaaaact taaaatatct taaaataagc gtcaatgctt
     1441 gaacatgaaa attatgttat aattatacat ataaaaaagt attgaggtgt ttgttttgaa
     1501 aaatttgtat aaaataaagg tgaaagacga cagctctgtn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnna ggtgtttgtt ttgaaaaatt tgtataaaat aaaggtgaaa
     1681 gacgacagct ctgtttcaga aaaaacctta tgtatacttt gtgcagatgt tcaggagata
     1741 ttcagactga ttccggcaaa catgaaagtc tcatcagtat cgcttgaggg tatctatcca
     1801 caaaataaga ggatcccgct taagtctatg aaaaagatag ttcagagcat atatctgatg
     1861 atgaacagtg gtataaatct ttatgactgt ctttttatga tagctgtagg ggatgagtac
     1921 gataagttta cccgcggggt ggtatgcaga agttactttc atatagtacg tggtgatgat
     1981 tacaaaaagt gctttgaatt ggctgagtat gacggttatt tttcccttgg gatggggata
     2041 tgcgaaacaa gggacatgtt tgtatccaac cttggaagtt ttttatcata ctatactgaa
     2101 aaagagaaag tacagtcgga actaaaaaaa tcacttctgt atccttttac cgttctttta
     2161 tcgcttacgg ttctgctttt ttttctgagg ttttatatca taccttcatt ttcttccttg
     2221 cttaatatag aaataaactt tacggtatct tcagtcctct ttgtatgctt ttttcttttg
     2281 cttattctga tatctgtttc atatgttttt gcacggaaaa atgattcata cctttgcaaa
     2341 atccctttct tgggagagtt ttataagaca atagtcttat atcgtttcgt aagggatata
     2401 aatcttctta tttcgagtgg acttactctt tatgaggcta tggataaaac tgttgaaagt
     2461 attgattcgg ttctggcaaa aaaagcattt ttaagtcccg gtctggatat acgtagcggg
     2521 cttgatgtaa aagaagcttt ctgtaaggta aaagatttta aagagatttc gctcgcattt
     2581 gccctgtcgg gaaaagtagg cggttacaga caggttcttg attttcttca aaaggactgt
     2641 cagagcagaa ttaaggaaag cagcgaaaaa attcttaaaa tgattgagcc cgttctcata
     2701 atacttattt ccgtacttat tatgagtatt gcctacgagg tttacagcaa ggtatttatt
     2761 ggaaacataa acttttcggt cggagagtag gtgaaatatg aagtccgttg agttcagaaa
     2821 aggtttttta atgctggaac tgagtatata tcttattgca gaagtgataa tgttctcatc
     2881 tctttgtgcc gtaagcattc ttcctctgta taggtacttt gaaagacttt ctgaccaggt
     2941 actggccgta aaaacgataa accgcatgag gactgtatct tttatttccc gcaaagcaga
     3001 tatttatgca agcaaaacat cggttattgc cggattcaga aataattact ttacgttcaa
     3061 aattaatcag tactcctata gagagctgac gtttgatgac ggatacataa agtcccggag
     3121 ggattatagt tgacaggaag aatcaaaccg tgctgaaagt ggttcctgta accgcggcgg
     3181 tgacgttacc gtgaaaaaaa caatattttt gaagtacaat ggaaagaaga taggagtagg
     3241 tcatatggac tgttacatac agtcatacca gacagagcag caccttttga aggatctttt
     3301 caaaaagggt atcagtaaaa gagtcgggtt ctttgaaatg ggaagctggg atgaaaagta
     3361 ctatttaatc tatgacaagt cgcattggct taaaaagaga ttatcgccta ttcaggatat
     3421 attcatcccg cagcaactgt tggcagtaga taccttgctt aaaatgccgt ctggagagta
     3481 tgttataaaa tttccttgct gctcatactt ttttgaaagg aataaatatt acattaagaa
     3541 cggaatcgtt tgtgataggc atgagtatga tgtttcattt tctgttcagg atattaataa
     3601 agcagtgttg gaatatgcta aaaagggcgg gctatgattt tttctgtaaa gagtttatca
     3661 aggaaaccgt ttttactgct tcttgattta ttgataatcg ttctggcggt tatgactgca
     3721 tactcttttt ttggcggagt atccagccat aaaaaagtcg ccgatgaaaa aaaatatgaa
     3781 aagagcaagt atgagaagta tctcaaaagc atcagggaag agaagaatga gatatctgag
     3841 tttttcagag ttcttgaaaa atatgatgca agactgatag agttcacatt tgtgggtgga
     3901 aggatagaga gtacggttat gcttgagctc tcagaaggtc aggagataat cacgaagtat
     3961 gcggtaaaaa agatttctga gttgaaaaca ggagaaaaga gcatagtctt tctcagtata
     4021 tttactgaat aaggagagtt tatgagaata agggctttgg ttgccttagt actgctggct
     4081 ttctttttga tactttcaat gctgtccaag gaggaacctt atgaggagca ggtctttata
     4141 ctgtacagca tcggaagcgg ttcgatagac agaaaatttt caaataaaga gcttaaaaaa
     4201 tttatgagtg ctgttttggc tgacgactca aaggttgaca gtataaaact gctcagagaa
     4261 aacagcagcg tgaataatat ctctactgac tttaccctac aggttaagca aagtctgcca
     4321 tctgagggtg agtttgctta taaaacactt atctgtgctg aaagtgccga aatagcagag
     4381 agcatatttg acggaaaggt cttttctctt gagtatctgt ggtcgatgaa agattcattc
     4441 aaaagtttta tgccagactg tataagattt gccggagtaa gggtgaaaaa cggagtaaaa
     4501 agtgcataca tctactttaa tgatgaactc ctaaatgtat gtgaaggaga ggttatcgga
     4561 gacagacaga tcatgggaat attcgatgac ggaatacttg ttctcacttg tggaaaagag
     4621 attgaggtaa tcttatgaaa aaaatttttc tagtcatagg cttattgctt ttaatagggc
     4681 tttcttttac aatgagcata gattatgata aaaaaacaaa cgccaacgta atcctgtttg
     4741 attttcaaaa ggagtttaca gtggtttcaa acagtgagct gacggttcat aagatacttt
     4801 tcggagaaga actgtttatc ccacagtcat acaaaattga aaaaggtcct ttgagatctg
     4861 tcgaaatact ttccaatatg ttggagataa gaactcttat gccagtaaag cctttagttg
     4921 acggcaaaaa ccgtctgata ttcaacggaa gaggtgcatt gaactttgaa aagctttttt
     4981 taaaagaaat aaagctggga gatcttctta atcttttttc acaggagctg aaattaaact
     5041 ggataaggat ggagccattt cctcaggaaa aattgagttt gtggtcagat gaga
//
LOCUS       DAOSHR010000146         4958 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_15128_length_4958_cov_4.253722, whole genome shotgun sequence.
ACCESSION   DAOSHR010000146 DAOSHR010000000
VERSION     DAOSHR010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 4958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4958
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15128_length_4958_cov_4.253722"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            124..693
                     /locus_tag="PLU28_08160"
     CDS             124..693
                     /locus_tag="PLU28_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35070.1"
                     /translation="MTERYQNVLRYLGYTSIEKSEAFIEQVMALFDELVKDSVPRNIY
                     GIYDLELKDKWVSMGKGTVVFNSGHLAGHLAGCRECAVVAVTLGALVDRKISYYQNVD
                     MSKAVIYNACANVYVEEVLSSTAVQIRKDAGALQSTFPFSPGYGDLGLEVQKEIIELL
                     QTKKSIGLYVNESCLLIPEKSITAVIGLK"
     gene            710..3064
                     /locus_tag="PLU28_08165"
     CDS             710..3064
                     /locus_tag="PLU28_08165"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HOG35071.1"
                     /translation="MFEDNKIIVFDGAMGTMIQKYGLKGSKIPELYNITDPDIIQQIH
                     GKYVDAGCDIITTNTFGANSNKLLNSLYSVKDVIQAAVTNAKKVSQGRKVALDIGPLG
                     MLMEPSGPMSFEQAYELFKEQVLAGTEAKVDLIIIETISDIYEAKAAILAAKENSNLS
                     VICTMTLGQDGRTFTGTDILTMVNVLQGLGVEALGLNCSLGPDEMYNHVKKVLEYSSV
                     PVIVQPNAGLPKIRNGQTVYDITAGYFAQRLCDMVDMGVRIVGGCCGTDERFIKELAE
                     KVKNKVPKKTVPKEFTMISSYCRTVVVDDGFTIIGERINPTGKPKLKRALEEKDYSYI
                     LSEAVKQKEEGAEILDVNAGLPGIDEKTVLPHIIRQIQKVCDIPLQIDSSSYETLENA
                     VRVYNGKAIINSVNGKAESMEKVFPIVKKYGCCVIGLTLDEQGIPKTSGKRVEIAQKI
                     VSTAQKYGISKNNILIDCLVLTASAEQENIIETLKAVREVKQKLNVKTVLGVSNVSYG
                     LPSRELLNKTFFVMAMSYGLDCAIINTGNTEMMSVYDAFNVLTAKDKNSEKYIKKYAQ
                     KKIETFTDNNGNQFTLEYVLSKGLKEKIKEVTLAELNKRTPMQIIDEIISPLLQTVGK
                     KYEKAELFLPQLMQTAEAVKAAFEILKEHMKASGQTIRKEKILLATVRGDIHDIGKNI
                     VKVVLENHGYEVIDMGKDVSEQDILKTAIENKVRLIGLSALMTTTVSNMESTIKYIKN
                     NSDEFVFIVGGAVLNEEYASMIGADYYAKDALQAAEIVNSIFRE"
     gene            complement(3084..4898)
                     /locus_tag="PLU28_08170"
     CDS             complement(3084..4898)
                     /locus_tag="PLU28_08170"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG35072.1"
                     /translation="MKKPTFKSTKLFLKVLLKSFKLFFIAGKAQLILLLFFTVIQGLV
                     PIMIAQSTRTILDSLNILKDSSLTFAVVILVITWGFWNLINFIINPLSLHIQGNLTDK
                     LILSCNLKALETMRRINDLSTMEESRFFDEQQLLEQELAWRPVNLIVFTFSFIRGFIT
                     VLSMMVSLIPFHPLIPVLLLLSLMPQAVQLYRLQQEAFENMVERNPLSRKMQYWSHLL
                     LERTTFREAKLFGYESYAISKYQNLFSQIYEKSSADRKKKTVSTLMFFIIGAAGSTAC
                     FLWVISQFRTNNLSIGSISLFSTYIIISSQSFITLIQDGALLYDTCLFMDKYFSFIDR
                     KPALKNGNIKLKSISSIEFRNVTFYYPGAEHSALKNLSFKMEGKNQYAIVGENGSGKT
                     TILNLICRFYDPQDGQVLINGYPLSSLEINSYRNQISMIFQDYARYELTVKENISISD
                     TSKEPVVQKINDSIKNSGLYNKLSTFADGIDQQLGKKFENGTELSGGEWQKVALARSL
                     YRDCSLFILDEPSSSMDAISQAVFWKNISEYTKNKLSVMVTHKVSSLKNTNCIFVLKN
                     GSIAEQGRYSQLIEQNGLYSGLSNAESMEGVTSDHTDI"
BASE COUNT         1621 a          810 c         1083 g         1444 t
ORIGIN      
        1 agtatatttg ctctcttcag gttttgatgg gattcatctt tattttatga ataaacctca
       61 ggtagcaaga aaaataaccc agaacataaa aactgtcata aaatctttaa aaagtggtga
      121 agtatgacgg aaagatatca aaacgtcttg cgttatctgg gatacacatc tattgaaaaa
      181 tccgaagctt ttatcgaaca ggtgatggct ctttttgatg agctggtaaa ggattcggtc
      241 ccaagaaata tttacgggat ctatgacctt gaactgaagg ataaatgggt atccatggga
      301 aaaggaacag tggttttcaa ttcaggacat ctggcagggc atctggcagg ctgcagggaa
      361 tgtgcggttg ttgctgtgac tcttggtgct cttgtcgaca ggaagataag ttattatcag
      421 aatgttgata tgagtaaggc tgttatatat aatgcctgtg caaatgttta tgtagaagaa
      481 gttctgtcta gtacagccgt tcaaatcaga aaagatgccg gagctttaca aagtaccttt
      541 ccattcagtc cgggatatgg ggatcttggg cttgaggttc agaaggaaat aatcgaactc
      601 ctgcaaacaa aaaaaagcat aggactatat gtaaatgaaa gctgtctgct aatacctgaa
      661 aaatctatta ccgctgttat tggattaaaa taaagaaaga ggtggaagca tgtttgaaga
      721 taataaaata atagtctttg atggtgctat gggtacgatg attcagaagt atggactaaa
      781 aggatcaaaa ataccagagc tttacaatat aacagaccct gatataatac agcagattca
      841 tggaaagtat gttgatgcgg gctgcgatat cattacaacc aatactttcg gtgcaaacag
      901 taataagcta ttgaatagtt tatacagtgt gaaggatgtc attcaagcgg cagtgacaaa
      961 tgcaaaaaag gtctctcagg gcagaaaagt ggctctagac atcggtcctt tgggtatgct
     1021 tatggaaccg tcaggtccga tgagctttga acaggcttat gagcttttca aagaacaggt
     1081 tttagccggt acagaagcta aggttgatct tataataatt gaaaccatat cagatattta
     1141 tgaagctaaa gcagccatac ttgcggcaaa agagaactct aacctgtctg taatatgcac
     1201 gatgactctg ggacaggatg gaagaacctt tacaggaaca gatatactta ctatggtgaa
     1261 tgttcttcaa ggattgggag ttgaggcttt gggccttaac tgttctctgg ggccggatga
     1321 aatgtataat catgtaaaaa aagttctgga gtattcttct gttccggtta tagttcagcc
     1381 taacgcagga cttccgaaaa taagaaatgg gcagacggtt tatgacatta ctgccggata
     1441 ttttgctcaa aggctctgcg atatggtaga tatgggtgta cgtatagtag gaggctgctg
     1501 cggaacagat gaaaggttta taaaggagct ggcagaaaaa gtaaagaata aagttcccaa
     1561 aaaaacagtt cctaaggaat ttacaatgat ttcttcttac tgccgtacag tagttgttga
     1621 tgatgggttc actattatag gtgaaagaat aaatcctaca ggaaagccca agctgaaaag
     1681 ggctcttgaa gaaaaagatt atagttatat tctgagcgaa gctgtaaaac agaaagaaga
     1741 aggggcagag atactggatg taaatgcagg gttaccggga attgacgaaa agacggtttt
     1801 accgcatatt atcagacaaa tacagaaggt ttgcgatatt cctcttcaga tagacagttc
     1861 aagctatgaa accttagaaa atgctgtaag ggtttataac ggaaaagcca taattaattc
     1921 tgtgaacgga aaagcagagt ctatggagaa agtatttccg atagtaaaaa aatatggctg
     1981 ttgtgtgata gggctcactc tagatgaaca gggaatacct aaaacctctg gaaaaagagt
     2041 tgagattgcc caaaagatag taagtacagc tcaaaagtac ggcatatcta aaaataatat
     2101 tctgatcgac tgtctggttc ttacagccag tgccgaacag gagaatataa ttgaaacgct
     2161 taaggctgta agagaggtta agcaaaagct aaatgtaaaa acagtactgg gagtgagcaa
     2221 tgtttcgtat ggacttccct caagagagct tcttaacaag accttttttg taatggcaat
     2281 gagttatggt ttagactgtg caataattaa taccggcaat acagaaatga tgagtgttta
     2341 tgatgctttt aacgttttga ctgccaaaga taaaaattct gagaaatata taaaaaagta
     2401 cgcacagaag aagattgaaa cttttacgga taataatggg aatcaattta cacttgagta
     2461 tgtgctttca aaaggactca aggaaaaaat taaagaggtt acgcttgctg agcttaataa
     2521 aaggacaccg atgcagatta tagatgaaat catttcgccg cttcttcaaa cggttggaaa
     2581 aaagtacgaa aaggcagagc tctttcttcc gcagcttatg cagactgccg aggctgtaaa
     2641 agctgcattt gaaatactta aggagcatat gaaggctagc ggacagacca taaggaaaga
     2701 aaaaatactc cttgccacag tcagaggaga tatacacgat ataggcaaga atattgtgaa
     2761 agttgtactt gaaaaccacg gatatgaggt tatagatatg gggaaagatg tttctgagca
     2821 ggatatactt aaaacagcca ttgaaaataa agtcaggctc ataggattaa gcgcgcttat
     2881 gacaaccacg gtttcaaaca tggaaagcac tataaagtat ataaagaata actccgatga
     2941 gtttgtgttc atagtcggcg gagctgtgct gaatgaggag tatgcgtcca tgatcggagc
     3001 tgactactat gccaaagatg cactacaggc tgcggaaatt gtaaattcaa ttttcagaga
     3061 gtgaaatagc tttcatactt gacctaaatg tcagtatggt ctgaagttac accttccatt
     3121 gattcggcgt ttgacaagcc ggaataaaga ccattttgtt ctatcagctg gctgtacctt
     3181 ccctgttcgg cgatagagcc gttttttaat acaaaaatgc agttagtatt tttcaggctg
     3241 ctgactttat gggttaccat aacagatagc ttgtttttag tatattcgga gatatttttc
     3301 cagaaaacgg cttggcttat ggcatccata gaagaagagg gttcgtcaag aataaaaagg
     3361 gagcagtctc tgtataaaga tcttgccaga gcaacttttt gccattcgcc accggatagt
     3421 tcggttccgt tttcaaactt tttacctagc tgctggtcaa tgccatcggc aaaggtcgaa
     3481 agtttgttat atagtccaga atttttaatg gaatcgttta ttttttgaac tacgggttct
     3541 tttgaagtgt ccgaaataga tatattttcc tttacggtaa gctcgtatct tgcgtaatcc
     3601 tgaaaaatca tggaaatctg gtttctgtat gagtttattt caagagagct caaagggtat
     3661 ccgtttataa gaacctgtcc atcctgcgga tcgtaaaatc tgcatataag atttagaatg
     3721 gtcgtttttc cacttccgtt ttctcccact attgcatatt ggtttttacc ctccatttta
     3781 aagcttaagt tttttaaagc agaatgctct gctccgggat agtagaaagt aacatttcta
     3841 aattcaatgc tggaaatact ctttagcttt atatttccgt tttttaaagc aggtttgcga
     3901 tctataaatg aaaaatactt atccataaaa aggcaggtat cgtataaaag tgctccgtcc
     3961 tgtataaggg ttataaagct ctgtgagctt atgattatat aggttgaaaa taagctaata
     4021 cttcctatgg atagattatt agtccgaaat tgggagatta cccacagaaa acaagcagtt
     4081 gatcctgcgg caccgattat aaagaacata agggtagaaa cggtcttttt ctttctgtct
     4141 gctgaagatt tttcatatat ctgcgaaaaa agattttggt actttgatat ggcatagctt
     4201 tcatatccga atagctttgc ttccctgaag gttgtacgtt caaggagaag atggctccaa
     4261 tactgcatct ttcgcgaaag aggatttctt tcgaccatgt tttcaaaagc ttcctgttgt
     4321 aaccggtaaa gctgaaccgc ctgaggcata agcgacagaa gcaacaatac cgggataagc
     4381 ggatgaaaag gtatgagact taccatcatt gatagcacag tgataaatcc cctgataaaa
     4441 ctgaaggtaa atactatgag atttaccggc cgccatgcaa gctcctgttc caaaagctgt
     4501 tgttcgtcaa agaacctgct ttcttccatt gtagaaaggt cgtttattct gcgcatggtt
     4561 tcaagagcct tcagattaca agatagaata agtttatccg taaggtttcc ctgtatatgt
     4621 aatgataagg gatttataat gaagtttatc agattccaaa atccccaggt tatgactaaa
     4681 atcacaaccg caaaagtcag cgaactgtct tttaaaatgt tcaggctgtc gagaatagtt
     4741 ctggtacttt gggctatcat tatgggaacc agtccttgaa taacagtaaa aaaaagcaac
     4801 aggataagtt gcgcttttcc tgcaatgaaa aacagtttaa aagattttaa aagaactttt
     4861 aaaaataatt ttgtgctttt aaaggttggt tttttcatac attcatcctt ttttggtaat
     4921 tcatgatttc tctatatcgt acgactttct tatctata
//
LOCUS       DAOSHR010000147         4948 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_15169_length_4948_cov_6.338647, whole genome shotgun sequence.
ACCESSION   DAOSHR010000147 DAOSHR010000000
VERSION     DAOSHR010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 4948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4948
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15169_length_4948_cov_6.338647"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="PLU28_08175"
     CDS             <1..380
                     /locus_tag="PLU28_08175"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOG35073.1"
                     /translation="QDIIQMYECKTGSLFGLCLCLPSIICSESSECERLYHLGKQFGI
                     FFQIMDDLDDIHLSSEQAGKDTGKDGDKKTLLSQWSEEKVIEYSSSIYSNLVESFYNM
                     GFFKLSAFISDYGKNIIERVRQG"
     gene            377..2890
                     /locus_tag="PLU28_08180"
     CDS             377..2890
                     /locus_tag="PLU28_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35074.1"
                     /translation="MKKREEEVKNTELENEKEPEKTKNNPAVNFIKKIMLFTNRLKYK
                     IKRKTIIIAVIAIIVFIGIFIGVKVLLDLSAQGTNKVTQNIIANDIQVIIPSDAYTYK
                     KEVSISEINPNSTEYQNLKSMYNFYGKIYKIDPKDGKEDSSFVPMIIRYKIPNEIYFG
                     DNYTHFSLAYSSDSNPPVVSEFSGCRIIKEGNSYYVEAETFHFSKVGLVVKSAKESTY
                     GLRTITERVPGIEPDIIVIPGTDLNFSGYLSNTSTAENPYGQSLWAALFPSRSIWSYN
                     YPMVDTKSKVYYDSYSGYVNRTGNSSYIEFEAKRFIQELKRLPNREFDIIANSVGGLI
                     ARYALESDPTIHNVKNIVLIDVPNKGTNLANPLFYNFFYGKDPKLLAKLFSLTERTII
                     SVSHNVGSFLEQVNSYYRELLPNSKFIENLNSLGVRNDIRYMSITGNNPKIDEELSDS
                     EILRFYPEFQRDAGDGLVTHTSALLPQSSKTLISPDYSYDIYNNQEVLLSIKEFLDES
                     VPDLKVKPFEDDSFIESTKPVVLPTQPASKTDSQKPPVVPYKYFTTPKSYSYSELMSE
                     PTVVGAINESTVKLVYAQNTIFMETFNGIYSQSLKKILNKKLFGGSIIDGKYYAATDE
                     GVFVYDQTSAEFKKIDDIVPISDEAYYISKGNLLSVVHSGNISNIYLGNLLLSSDAKL
                     IKVKTIGQSVYLVFDSKISKLTENKLTTIVDKKTMALKLNVSIDSFSDFELSGSMIFV
                     LTNDYKLFMYDPQNNKQQLIGSSDIGRLKLSNYLNRKLYVFGGDITTYIDIQSRVFPG
                     VYNRMEKDIFDICLTPSGMLYGIYNSQEGKILWTSTLKE"
     gene            2866..4164
                     /locus_tag="PLU28_08185"
     CDS             2866..4164
                     /locus_tag="PLU28_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35075.1"
                     /translation="MDFNLKRVVLVGIFILMMCASVFCVEFDQAYSYYLSGMKAYRIG
                     DYKLSQNLLYKSIQVSPQLENYDANIKLYLGLSAFQNKDYAIALEYLPMFSENPLAVQ
                     ALESIKNNGISSEFHFKQATVPSTQEELDASEEKSSFPLLTFFLITFIIFVISAASAF
                     PIFIYFRKHGLSFSEKPKAEIQEIPALEEVASVPIEEVVNLKLDNIENIWKKSNALRM
                     LIGDIENEYESSSAPEIPEIPELVSTEHENLKTSDQTADIDNLQSIINDSLKTADLDS
                     IEKLLNSMENQNISESSQEITTEIPINDSIPNSPSYEAYASANDKEENQTSVNSDLTE
                     QDEKQSYPESENIQPVNPNKDVDILDAGSSLKTRFKDIMKGQEKMIFVEAQNTNDISE
                     IIERIESENSKNGSKIYSKIQLEKVLTNLFYQVNESELSK"
     gene            complement(4191..4265)
                     /locus_tag="PLU28_08190"
     tRNA            complement(4191..4265)
                     /locus_tag="PLU28_08190"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4231..4233),aa:Gly,seq:ccc)
     gene            4325..>4948
                     /gene="lgt"
                     /locus_tag="PLU28_08195"
     CDS             4325..>4948
                     /gene="lgt"
                     /locus_tag="PLU28_08195"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOG35076.1"
                     /translation="MFKDRTFTWVLIITISLFALVSFLFLPKAFSGEVVYSPEIFSIG
                     PLIVRWYGLLISSSILLCYFIGRHQAFKEKVSEDDLLNAVFIGIILGVIGARLYYVIF
                     NFSFFSDNPISILKIWTGGLAIHGGIIGAVVSTFLYTKYKKNCTLKFFQAMDLFTFVL
                     PLSQGIGRWGNFMNHEAYGTPTDFFLKMYVPLESRMPGLESFSFFHPT"
BASE COUNT         1660 a          903 c          876 g         1509 t
ORIGIN      
        1 cacaggatat aatacagatg tatgaatgca aaaccggttc acttttcggt ctttgcttgt
       61 gtctaccttc aataatttgt tctgaaagct ctgaatgcga acgtttatac catttaggga
      121 aacagtttgg tatattcttc cagataatgg atgatctgga cgatatccat ctttcatcag
      181 aacaggccgg aaaagatact ggaaaagacg gtgataaaaa gactctcctt tctcaatggt
      241 ctgaagaaaa agtcatcgaa tactcttcaa gtatctattc aaatcttgtg gagtcttttt
      301 ataatatggg tttttttaag ctttcagctt ttatatctga ttatgggaaa aatattattg
      361 aaagggtgag acaaggatga aaaaaaggga agaagaggtt aaaaatactg agctggaaaa
      421 tgaaaaggaa cctgagaaaa ccaaaaacaa tccggcagtg aattttatta aaaagatcat
      481 gctttttact aaccggttaa aatataaaat aaaaaggaaa accattataa ttgctgtcat
      541 tgcgataatt gtttttatag gtatttttat aggagttaaa gtacttcttg atctttcggc
      601 acagggtacg aataaggtca cccagaatat aattgcaaac gatatacagg tcataatacc
      661 cagtgatgct tacacctata aaaaagaggt tagtataagc gagattaatc cgaactctac
      721 agagtatcaa aatctgaaat caatgtataa cttctacggc aagatctata aaattgatcc
      781 taaagatggt aaagaagact cttcctttgt tcctatgata atacgctata aaatcccaaa
      841 tgaaatatat tttggggata actacactca cttctccctt gcttattctt cagattccaa
      901 tccacctgtt gtttctgaat tttcaggatg cagaattata aaagaaggaa acagttatta
      961 tgttgaggct gaaactttcc atttttccaa agttggatta gttgtcaaat cagccaaaga
     1021 aagcacctat ggactgagaa caataactga aagagtacct ggtatagaac ctgacataat
     1081 agtaattccc ggaacagatc tgaacttctc cggatacctt tcaaatacat ctactgccga
     1141 aaatccttat ggacaaagtt tgtgggcagc tcttttccct agccgaagca tatggtccta
     1201 caattatccc atggtagata ccaaaagcaa agtgtattac gactcttatt ccggttatgt
     1261 taaccgtacc ggaaacagta gttatataga gtttgaggcc aaacgtttca tacaggagct
     1321 taaacggctt ccaaacagag agtttgatat tattgccaac agtgtcggcg gtctgatagc
     1381 cagatatgcg cttgaatcag atcctacaat acataacgtt aaaaatatag ttctgataga
     1441 tgttcctaat aaaggaacca atcttgccaa tcctttgttc tataactttt tttatggaaa
     1501 agatcccaag cttctcgcaa aactgttttc acttacagaa aggactataa tatcggtttc
     1561 tcataatgtt gggtctttcc ttgaacaggt aaactcgtac taccgtgaac ttcttccaaa
     1621 ctcaaaattt atagaaaatc ttaactccct gggagtaaga aatgatatac gttatatgtc
     1681 aattacaggg aataacccca aaatagatga agaactgtct gattcagaaa tactacgttt
     1741 ttatcctgaa tttcaaagag atgcaggaga cggtttggtt acacacacaa gcgctcttct
     1801 gccacagtcg tcaaaaacac ttatatctcc ggattattcg tacgatatct ataataacca
     1861 agaagttttg ttgtccataa aagagtttct ggatgagtct gtacctgatc tgaaggttaa
     1921 gccttttgaa gatgattcct tcatagaaag taccaaacct gttgttttac caactcagcc
     1981 ggcctctaag accgattctc agaaaccacc ggtagtaccg tacaaatact ttactacacc
     2041 taagtcatac tcatattcag agcttatgtc agaaccaaca gttgtcggag caattaacga
     2101 atcaacagtc aaactggtat atgcgcagaa caccattttt atggaaacct ttaacggaat
     2161 atactctcag tctcttaaaa aaatacttaa taaaaaacta ttcggaggct caataattga
     2221 tgggaagtat tatgcagcaa ctgatgaagg agttttcgtt tatgatcaga cttccgctga
     2281 gttcaaaaag atagatgata ttgttcctat ctcagatgaa gcctactata tctccaaggg
     2341 taatttgtta agtgtagttc atagcggaaa tatttcaaat atataccttg gtaaccttct
     2401 tttaagttcg gatgcaaagc tcataaaagt taaaaccata ggtcagtctg tttatctggt
     2461 atttgactca aaaatttcca aacttacaga aaacaagctt actacaatag ttgataaaaa
     2521 aacgatggca ttgaagttaa atgtttccat agacagtttt tcggattttg aactttcagg
     2581 atctatgatc tttgtgctta cgaatgatta caagctgttt atgtacgatc ctcaaaacaa
     2641 taaacaacag cttataggaa gttcagacat aggaagatta aaactctcca actatttaaa
     2701 tagaaaactg tatgttttcg gcggcgatat tactacatat attgatattc aaagcagagt
     2761 atttccggga gtttataaca gaatggaaaa agatattttt gatatctgcc tcacaccatc
     2821 cggtatgttg tatggaattt ataattctca ggaaggtaaa attctatgga cttcaacctt
     2881 aaaagagtag ttttggtggg gatctttatc ctcatgatgt gtgcatcggt attttgtgtg
     2941 gagtttgacc aggcatattc atactatctt tcaggtatga aagcttatag gataggcgat
     3001 tacaaactat cccaaaatct tttgtataaa tcaatacagg tctcacctca attggaaaat
     3061 tacgatgcca atataaaact ttacttgggt ctttcagcat tccagaacaa agattacgca
     3121 atagcgctag aatatctgcc gatgttttcc gaaaaccctc ttgctgtcca agctcttgaa
     3181 agcataaaaa ataatggtat ttcttctgaa tttcatttca aacaggcaac tgttccatct
     3241 acacaggagg aattagatgc ttctgaagaa aaaagtagtt ttcctcttct tacttttttc
     3301 cttataactt ttataatatt tgttatatct gctgcatctg cttttccaat attcatttat
     3361 ttcagaaaac acggactatc cttttctgaa aaacctaaag cggaaattca ggaaatacca
     3421 gcacttgaag aagttgcttc ggtacctata gaagaagttg tcaacctaaa acttgataat
     3481 atagaaaata tatggaaaaa atcaaatgca ctgagaatgc tcatcggaga tatagaaaat
     3541 gaatacgaaa gttcctctgc tcctgaaatc cctgagatcc ccgaactggt ctctacagaa
     3601 catgaaaatc ttaaaacaag cgaccagaca gcagatatcg ataacctaca gagtataata
     3661 aatgattctc taaaaacagc cgatcttgac agtattgaaa agcttctcaa cagtatggaa
     3721 aaccagaata tttcagaatc ttctcaggaa ataaccacgg aaatccctat taatgatagc
     3781 attcccaatt ctccttcata cgaagcttat gcttctgcca atgataaaga agagaatcag
     3841 acttctgtaa actctgattt aactgaacag gacgagaaac aatcatatcc agagagcgag
     3901 aatattcagc ctgtaaatcc caataaggat gtagatatac ttgatgcagg ttcttccttg
     3961 aaaacaagat ttaaagatat aatgaaggga caggagaaaa tgatattcgt tgaagctcag
     4021 aataccaacg atatttctga gataatagaa cgtatagaat ctgaaaattc gaaaaacggc
     4081 tcaaaaatct attcaaaaat tcaacttgag aaggtactaa caaacctatt ctatcaagtc
     4141 aacgaaagcg aattaagcaa ataaaaacag tctcatttaa gagactgtcc tggagcgggc
     4201 aacggggctt gaacccgcga ccctcagctt gggaagctga tgctctaccg ctgagctacg
     4261 cccgcatgat acttacaata tgtatattat accaaatttt tttaaaaaat caaggaggaa
     4321 aactttgttt aaggacagga cttttacttg ggttctcatt atcacaattt ctctttttgc
     4381 attggtatca tttctttttc ttcccaaagc tttttcggga gaggtcgttt acagtcctga
     4441 aatattcagt ataggacctc tcatagtcag atggtacgga cttctaataa gctcttccat
     4501 acttttatgc tattttatag gtagacacca ggcctttaaa gaaaaggtat ctgaggatga
     4561 tcttttaaat gcggttttca taggtataat tttgggagtt ataggagcaa gactttatta
     4621 tgttatcttt aattttagtt ttttttcgga caaccccatc tctatattaa aaatatggac
     4681 cggtggactt gccattcacg gcggaataat aggcgcggtg gtttcaactt ttctatatac
     4741 caaatataaa aagaactgca cccttaagtt tttccaggca atggatctct ttacctttgt
     4801 gcttccactc tctcagggta tcggccgttg gggaaacttc atgaatcatg aggcttacgg
     4861 cactccaact gatttttttc tgaagatgta tgttcctttg gaaagcagga tgccaggtct
     4921 ggaaagcttc agttttttcc atcctact
//
LOCUS       DAOSHR010000148         4930 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_15252_length_4930_cov_5.584821, whole genome shotgun sequence.
ACCESSION   DAOSHR010000148 DAOSHR010000000
VERSION     DAOSHR010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 4930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4930
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15252_length_4930_cov_5.584821"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..894)
                     /locus_tag="PLU28_08200"
     CDS             complement(<1..894)
                     /locus_tag="PLU28_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOG35077.1"
                     /translation="MSLDNLTPKMIVNELDKYIVGQASAKKLVAIALRNRIRRMKLSE
                     DIRREVIPKNILMIGPTGVGKTEIARRLAMISDSPFVKVEATKFTEVGYVGKNVESII
                     RDLVDISIANIKSKMMQEVEDIAKGRVEERIAQSILPGKKEQKKQSMSDFMQMFTGQK
                     NEEVQYQNVDYDEIQKRDELIQRIKSNELDDQEIEVEVEEEQVPSIPGLGPDMEDMGL
                     QLGEMFSSFMPKKKKIRKMKVSQAKKVLLPIEEEKLIDKDRMIQQGIEAAQNRGIVFI
                     DEIDEIVSRGSSSGPDVSREGV"
     gene            complement(979..2406)
                     /gene="pyk"
                     /locus_tag="PLU28_08205"
     CDS             complement(979..2406)
                     /gene="pyk"
                     /locus_tag="PLU28_08205"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HOG35078.1"
                     /translation="MDSRKTRIVCTLGPATDSEENIKKLLLNGMNVARLNTSHGTVEE
                     HFSRVEKLKKIRKELGLPVAILIDLEGPKIRTGKFMRDTVELIENNEFILTSRDILGD
                     EKSVSVSYKELVRDLNKKDIILLNDGKIKLEVLSCDETDIKTKILVGGSITHRRGVNI
                     PGVDIKLAALTDKDMLYLENSVKWEVDYIAQSFVRKSSDIMQTKEILAKLGAPDMPVI
                     AKIETLQAIDNLESILEVSDGVMVARGDLGVEVPVQSVPILQKKIINMANIKAKPVIT
                     ATQMLETMIENPFPTRAEATDIANAILDGTDAVMLSAETSVGKYPFESVNVMVKVALE
                     TERYFGQFQNKINEQYIECGDKATNALSKSAVDISQTINAKVIVATTYSGYTARALSR
                     FRKNITIIAASPREETYNRLALVWGVTPVIMSKFVDTDAMLEKVSNIVKSLGLGVKGD
                     LIIVTAGIPYGFSGATNLLKVHQIQ"
     gene            complement(2421..3386)
                     /gene="pfkA"
                     /locus_tag="PLU28_08210"
     CDS             complement(2421..3386)
                     /gene="pfkA"
                     /locus_tag="PLU28_08210"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOG35079.1"
                     /translation="MPIKRIGIITSGGDAPGMNAALRSVVRTAVTNGIEVYGFMRGYA
                     GILDKDYKPLDYASVGGIMERGGTILRTARVPEFKLPEVRKQAADNIKEAGVEALVII
                     GGEGSLTGGKLFSEENGIPVVGIPGSIDNDISNTDMCIGVDTCLNTVVEVVQKIKDTA
                     TSHERAFIVEVMGRGSGYIALMSSLATGAEAAIIPEIPVDFKSISERIWEERKRGKLN
                     CLIIVAEGAASAYTVARHMENRIGYETRITILGHVQRGGSPTAYDRILASRMGYEAVK
                     SIMDNEFGVMIGLSGRNMVRVPIDKVLSEKRTIDVEVIKLAKILS"
     gene            3609..4046
                     /locus_tag="PLU28_08215"
     CDS             3609..4046
                     /locus_tag="PLU28_08215"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HOG35080.1"
                     /translation="MKTLDRGSILEILKDHNIKITPNRLVVAEVILNSISHPSIDEIH
                     TKLIRDGRNISFTSVYNIIKTFEHAGLLKEIQTHDRCRYDPNIKPHIHFICKTCSRVY
                     DIDSLSYNIKLPENILGMKVESYELTYYGYCSECLLDKLDKDE"
     gene            complement(4070..>4930)
                     /gene="gcvPB"
                     /locus_tag="PLU28_08220"
     CDS             complement(4070..>4930)
                     /gene="gcvPB"
                     /locus_tag="PLU28_08220"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HOG35081.1"
                     /translation="DLKMNMTDEVAAIMLTNPNTLGIFEKNICEIQKIAHEKNALLYY
                     DGANLNAIMGKVRPGDMGFDVVHLNLHKTFSTPHGMGGPGSGPVGVKKHLSPYLPKPV
                     VEMDIDGRYYFNNDMPLSIGKVRSFYGNFSVMVRAYTYILMMGKDGLRRASELAVLNA
                     NYMRVKLSKSFKISHENICKHEFVINGSALKEYGVKTLDFVKMLLDNGIHPPTVYFPL
                     IVEEAMMIEPTETENKETLDNTIKVFEDILKVAKTDPQKLHDAPFNTPVRRLDETKAT
                     KDLKVRYSIK"
BASE COUNT         1399 a         1075 c          734 g         1722 t
ORIGIN      
        1 tactccttca cgcgatacat caggacctga tgaacttccc cttgatacaa tctcgtctat
       61 ttcatctata aaaacaattc ccctgttttg tgctgcctct attccttgtt gaatcattct
      121 gtctttatct ataagttttt cctcttctat aggaagaaga actttcttag cctgtgaaac
      181 cttcatcttt cttattttct tttttttggg catgaaggaa gaaaacatct ctccaagctg
      241 caaacccata tcttccatat ccgggcctaa acctggtatg gaaggtacct gctcttcctc
      301 aacctcaacc tcaatttcct gatcatccaa ttcattactt tttatacgtt gaattaactc
      361 atcacgtttt tgaatttcat cataatcaac attctgatat tgaacttctt cgtttttctg
      421 accggtaaac atctgcataa aatcactcat tgactgcttt ttttgttcct tcttacctgg
      481 aagaatactt tgagctattc tttcttcaac ccttccttta gctatgtctt caacttcctg
      541 catcatctta gattttatat tggcaattga aatatcaaca agatctctta ttattgattc
      601 aacattcttg cctacatacc caacttctgt aaactttgta gcttcaacct ttacaaaagg
      661 tgagtcagat atcattgcca gtctccttgc gatttcagtc tttccgactc ctgtaggtcc
      721 tatcataaga atattttttg gaattacctc gcgtctgata tcctctgaaa gtttcatcct
      781 tcttatacgg tttctcagag ctattgccac taattttttt gccgatgcct gtcctacaat
      841 atatttatca agctcattta ctatcatctt tggagtcaga ttatccagag acatactatc
      901 acctccgcac tatattttat cataaataga tgaaaagaaa taaaggaccg atatgccatc
      961 gatcctttat tatttttttt attgaatttg atgtactttc aaaagattcg tggccccaga
     1021 aaatccatat ggaattccag cagtaacaat tatcagatcg ccttttactc caagtcctaa
     1081 agacttgact atatttgata ctttttcgag catagcatca gtatcaacaa actttgacat
     1141 tataaccgga gttactcccc agaccaaagc cagtctgtta taagtttctt ctcttggaga
     1201 agcggcaata atggttatgt tttttctgaa ccttgaaagc gcacgggcag tatagccact
     1261 ataagtggtt gctacaatta ctttggcatt tatcgtctga gatatgtcca cagcagattt
     1321 tgaaagagca tttgtagctt tatcgccgca ttctatatac tgttcattta tcttgttctg
     1381 aaactgtcca aagtatcttt cggtttcaag tgccaccttt accattacat tgacagattc
     1441 aaacggatac tttccgacag aagtttctgc agacagcata accgcatcgg taccatccaa
     1501 tatggcattg gctatatctg ttgcttcagc tcttgtggga aaaggatttt ctatcatggt
     1561 ttcaagcatc tgagttgcag ttataacggg tttggccttt atatttgcca tgttaatgat
     1621 ctttttttgt agtatgggaa cagactgtac gggaacttca actccaagat ctcctctagc
     1681 taccatcaca ccatcggaaa cttcaagtat gctttccaga ttatctatag cctgaagagt
     1741 ttctatttta gcaattacag gcatatcagg cgcacccagc ttggcaagaa tctctttagt
     1801 ttgcataata tccgaacttt tccttacaaa ggactgggca atataatcta cttcccattt
     1861 aacagaattt tcaagataca gcatgtcttt atcagtaagt gctgcgagtt ttatatcaac
     1921 tccgggaata tttactcctc ttcggtgagt aatacttcct ccaaccagta tcttcgtctt
     1981 tatatcagtt tcatcacaag ataaaacttc aagttttatc ttaccgtcat ttaataaaat
     2041 tatatctttt ttatttaggt ctcttacaag ttctttataa ctaacactta cagatttttc
     2101 atctcccaaa atatctcttg aagtaagtat aaactcatta ttttcaataa gctcaactgt
     2161 atctctcata aactttccag ttcttatttt gggaccctca aggtcaatca gtatagctac
     2221 aggaagtccc aactctttac gtatcttctt tagcttttcg actctggaga aatgttcttc
     2281 aacagtacca tgtgatgtat ttagtctggc cacattcatg ccgttcagca aaagtttttt
     2341 tatattctcc tcactgtcgg ttgccggccc taatgtacat actattctgg tctttcttga
     2401 gtccattact tttccccctt ttaggaaagt atttttgcaa gctttatgac ttccacatct
     2461 atagtccttt tttcggaaag aactttatcg ataggcacac gtaccatatt tctcccgctc
     2521 agtcctatca taacgccaaa ttcattatcc atgatgcttt tcactgcctc gtatcccatt
     2581 ctggaagcaa gtattctgtc gtaagcagta ggacttccgc cacgttgaac atgaccaagt
     2641 atggttattc ttgtttcgta tcctatcctg ttttccatat gtctagcaac cgtgtaagca
     2701 ctggcagcac cttctgcaac tattataagg caattaagtt ttcctctttt cctttcttcc
     2761 catatccttt cggagataga tttgaaatca acaggaatct caggaattat agcagcttct
     2821 gcacctgtag caagactaga cataagagca atataaccgg aacctcttcc cattacttct
     2881 acaataaatg ctctttcatg tgatgtagcg gtatccttta tcttctgtac aacttcaaca
     2941 accgtattaa gacaagtatc cacaccaata cacatatcag tattggaaat atcgttatct
     3001 attgaaccgg gaatacctac aaccggaatg ccgttttcct cagaaaaaag ctttccaccg
     3061 gtaaggcttc cttcccctcc gattattact aaagcctcta ctccagcttc tttaatatta
     3121 tctgcagcct gttttcttac ttcaggaagc ttaaattcag gaaccctagc tgttcgcaga
     3181 attgttcctc cacgttccat aattccgcca actgaagcat aatcaagtgg cttataatct
     3241 ttatcaagaa tacccgcgta acctctcata aatccatata cttctattcc atttgtaaca
     3301 gctgttctta ctaccgatct gagagcagca ttcattccgg gcgcatctcc tccgctggtg
     3361 attattccta ttctctttat gggcatactc gggcctccta aatataaaat atatgaataa
     3421 ttttctaagt gatattatat catcattttg ataacttttg gcattacaaa aattaagtct
     3481 tattttttat gatagcttta tgttttattt gatatgtaaa tatatttgcg cttaaatgta
     3541 aaattttaaa ttgatattta aaactgagct cctttatgct ataataagta aaaaggaggt
     3601 atagtaaatt gaaaaccctt gaccgcggct ctattcttga aatacttaaa gaccataata
     3661 taaaaattac tccaaacaga cttgttgtag cagaagtaat tcttaattct atctctcatc
     3721 cttcaataga cgaaattcat accaagctta tcagagatgg aagaaatatt tcttttactt
     3781 cagtttacaa cataataaaa acctttgaac atgccggact tttaaaggaa atacaaactc
     3841 atgacagatg tcgttatgat ccgaatatca aaccacatat ccattttata tgcaagacct
     3901 gttccagagt ttatgacata gattcactca gctataatat aaagcttcct gaaaatatct
     3961 tgggaatgaa agtggaaagc tatgaactaa cttactatgg atactgctca gaatgtcttc
     4021 ttgacaaatt ggataaagat gaataaaacc cgcatatatg cgggttttat tattttatac
     4081 tatatcggac tttaagatct tttgtagcct tggtttcatc taaacgtctt acaggagtat
     4141 taaagggagc atcatgcagt ttctgtggat cagttttggc aactttaagt atatcttcga
     4201 atacttttat agtattatca agggtttctt tgttctctgt ctctgtaggc tcaatcatca
     4261 tcgcttcctc aacaattagc gggaagtata ccgttggtgg atgaataccg ttatccaaaa
     4321 gcatttttac aaaatccaga gtctttacac catactcttt cagggcagat ccattgatta
     4381 caaattcatg cttacatata ttttcatgag atattttaaa tgattttgaa agctttaccc
     4441 tcatatagtt agcattgagt actgcgagct cacttgctct tcgtaatccg tcttttccca
     4501 tcataagtat gtatgtgtag gctctgacca taacactgaa gttgccgtaa aagcttctta
     4561 ccttacctat gcttaatggc atatcgttat tgaaatagta tcttccgtca atatccattt
     4621 caactaccgg tttaggcaag tacggactca aatgcttctt tacacctacc ggtcctgaac
     4681 caggtcctcc cattccgtga ggggtagaaa atgtcttatg aagattcaga tgcacaacat
     4741 caaaacccat atctcccggg cgtacctttc ccataatagc gttgagatta gcaccgtcat
     4801 aatacaaaag cgcatttttc tcatgagcaa tcttctgtat ttcacatata tttttttcaa
     4861 atattcctaa tgtattcgga ttggtaagca ttatagcagc aacctcatcc gtcatattca
     4921 tttttaagtc
//
LOCUS       DAOSHR010000149         4925 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_15277_length_4925_cov_5.085216, whole genome shotgun sequence.
ACCESSION   DAOSHR010000149 DAOSHR010000000
VERSION     DAOSHR010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 4925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4925
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15277_length_4925_cov_5.085216"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /gene="pyrR"
                     /locus_tag="PLU28_08225"
     CDS             complement(<1..432)
                     /gene="pyrR"
                     /locus_tag="PLU28_08225"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003549.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HOG35082.1"
                     /translation="MILKNKVMDSKDIQRTLMRLTHELLERNRGVENTVLLGIKKGGK
                     EICDRIWNNIYKIENIYLEKGYIDVSPYRDDEKKNQEDTSEINFALTDKTVVLIDDVL
                     FTGRTVRAAMEAIVNLGRPKNIQLMVLIDRGHREFPIKADYV"
     gene            complement(544..1578)
                     /locus_tag="PLU28_08230"
     CDS             complement(544..1578)
                     /locus_tag="PLU28_08230"
                     /inference="COORDINATES: protein motif:HMM:NF040257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35083.1"
                     /translation="MFREKIRVVQYGCGKMSKYILRYLYENGAEIVGAIDVNPKVVGM
                     DIGDFAELGVKLGVKIRSDADAVLDECDPDIAVVTLFSFMNDVYPHFEKVLSRGINLI
                     STCEEAIYPWTTSAALTNKLDRLAKENNCTAVGSGMQDIFWVNMIALVAGGVHKINKI
                     EGAVSYNVEDYGLALAKAHGVGLSPQEFEATLAHPQTLEPSYVWNSNEALCSKMGWTI
                     KSQTQKCVPYFYDKDLFSSTMGKVIPKGMCIGMSAVVTTETFQGPIIETQCIGKVYGP
                     DDGDMCDWKISGEPDTTFFVKKPATVEHTCATIVNRIPTVLKAPAGFYTAEKLDTIQY
                     LAYPMHFYLD"
     gene            complement(1676..2518)
                     /locus_tag="PLU28_08235"
     CDS             complement(1676..2518)
                     /locus_tag="PLU28_08235"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOG35084.1"
                     /translation="MEKLTIGKMAKINHISEQTLRLYDKLDLIKPDEINAENGYRYYS
                     IRQCARLDMIQYMKSLGMKLIDIKKLLDQNDISLFKSILERKSNQIDLQIKEMLYQKR
                     ALQRTIESYERYEKSPPDGSIVMEFISKRQMYCIDAGINFYEYGIEVYEEMLRKLKNK
                     LISDSLPQIYFCNAGTIMRKSELEKRRLYSSEVFVFVDEEFVDKNLITYIPADTYACI
                     YCDSFNKEKEYLLRILDTIEQKKFIITGDYLCEVIADLPVFEKQERGMYLKLQIPVKF
                     SEES"
     gene            complement(2579..3508)
                     /locus_tag="PLU28_08240"
     CDS             complement(2579..3508)
                     /locus_tag="PLU28_08240"
                     /inference="COORDINATES: protein motif:HMM:NF013292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HOG35085.1"
                     /translation="MKWGVIATWRMALDGIKKAGLMLDQGSSIFDALQQVITEVEDCP
                     DYTSVGYGALPNEKGIIELDAAFMDGTTLSFGAVSGIVNFKNPVKIARKLMDERFNIF
                     LSGQGAQEYARINGFEEKNMITESSYAIWQKKLLEKKDALKPYDGHDTVGAVGIDTSS
                     HMAAATSTSGLFMKKTGRIGDSPLPGCGLYSDSNIGSAAATGLGEDIMKGCVCYETVR
                     LMESGLSPQDAAKRAVENFNKKLMEKRGQAGDISVVCMNNDAQWGAATNTGHFSFVTA
                     TYSEKPTVYILSFHDGKQILLPATDEWIRNNKE"
     gene            complement(3526..4272)
                     /locus_tag="PLU28_08245"
     CDS             complement(3526..4272)
                     /locus_tag="PLU28_08245"
                     /inference="COORDINATES: protein motif:HMM:NF015866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HOG35086.1"
                     /translation="MKPILVEVCADSLESAQIAQKAGAQRIELCSALSCGGLTPSAAL
                     MEYTKKKVTIPIHVLIRPRSGDFLYSDDEIQIMCRDIEIAKSVGVNGIVCGVLTPQGT
                     VDIKKLQTLLEAAYPLKISFHRAFDMLKDPYACAAVLKDMGIERILTSGQKPSAVQGS
                     KMISDLINIFGNELIIMPGAGINEKNIKAVITATGATEVHLSGKTIKNSDMIFRNESV
                     AMGSPHSLSEFEHYIADEYIVSRAIENINR"
     gene            complement(4269..>4925)
                     /locus_tag="PLU28_08250"
     CDS             complement(4269..>4925)
                     /locus_tag="PLU28_08250"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HOG35087.1"
                     /translation="NSIVHVMNTSDTVTLRGPYKTWKGEGVLREEFDGFLFQIPPTSF
                     FQNNYNVTHKMLNYILKYFKTLNTASDSLLDLYCGVGLFSIYFAPLFKTVYGVETSKV
                     SIKAACSNSNINSVRNTRFSSSKSEDFILQSCIESKKFSYAIIDPPREGIGVLSAKNL
                     AKITEKAIVYISCDPTTLARDIRAFMESGFEVLSVQPFDMFPNTYHVETVVILEKKNV
                     "
BASE COUNT         1425 a         1017 c          836 g         1647 t
ORIGIN      
        1 cacataatcg gcctttatag ggaactctct atgaccacgg tctataagaa ccatgagttg
       61 aatattttta ggtcttccca ggtttactat agcttccatc gcagccctca cagtccttcc
      121 tgtaaaaagg acatcatcaa taagtactac tgttttgtca gtaagcgcaa aatttatttc
      181 agaggtatcc tcttgatttt tcttttcatc atctctatat ggcgatacat ctatataccc
      241 tttttcaagg tagatatttt caattttata aatattattc caaattctgt cacatatctc
      301 ttttccgccc tttttaatac caagaagcac ggtgttctcg acacctctgt ttctttcgag
      361 cagttcatgg gtaagtctca tgagagtgcg ctgaatatct tttgaatcca taactttatt
      421 ttttaagatc atatatcctc cgtaaacact taatgtaata caattatacc attagtaaag
      481 aaataataat ctaaaaataa gataaaaaaa gcagcggtat taaaccgctg cttgtgattg
      541 attttaatca agataaaagt gcatcggata agcaagatac tgaatagtat caagcttttc
      601 tgcagtgtag aatcctgcgg gagctttaag aaccgtaggt atcctgttta ctattgtagc
      661 acatgtatgt tcaacagtgg caggtttttt tacaaaaaat gttgtatctg gttctccaga
      721 gattttccaa tcacacatat cgccatcatc cggaccgtat acttttccta tacactgtgt
      781 ttcaattata ggtccctgga aagtttccgt ggtaaccaca gcgctcatcc ctatgcacat
      841 tccctttgga attactttac ccattgtgga agaaaaaaga tctttatcgt aaaagtacgg
      901 aacacatttc tgagtctgag attttattgt ccatcccatc ttagaacaca atgcttcgtt
      961 tgaattccaa acgtatgaag gctcaagagt ctgaggatgt gctaaagtag cttcaaactc
     1021 ctgaggtgaa agaccaacac catgagcctt ggcaagagca agtccataat cttcaacatt
     1081 atagcttacg gctccttcta ttttgtttat tttatgtact cctccggcta ccagtgcaat
     1141 catattcacc cagaaaatat cctgcattcc tgaaccgacg gcggtgcagt tgttttcttt
     1201 tgcgaggcgg tcaagtttat ttgtaagggc tgccgaagta gtccaaggat aaattgcttc
     1261 ttcacatgtc gaaataaggt ttattcctct gcttagtact ttttcgaagt ggggatatac
     1321 atcattcata aagctgaaaa gtgtaactac tgcaatatca gggtcacact catctaatac
     1381 tgcatcagca tcgcttctga ttttgacacc caattttact ccaagttcag cgaaatctcc
     1441 gatatccatg cctactactt tggggtttac atcaatagcg cctacgatct ctgcaccatt
     1501 ttcgtaaagg tatctcagaa tatacttgga catctttccg catccgtact gtacaactct
     1561 tattttttct ctgaacataa gaaacacctc actttttatt ttttttgaat caaaagatcc
     1621 gtgattctat attcaattat aatggttatt gtaagtataa agtcaagcga aatttttaac
     1681 tttcttccga aaattttaca gggatctgta atttaagata catcccgcgt tcctgttttt
     1741 caaataccgg aagatcggct attacttcgc atagatagtc accggtaata ataaattttt
     1801 tttgttcaat agtatccaaa attcttaaga gatattcttt ttctttgtta aaactatcac
     1861 agtatataca ggcataggtg tcagccggga tataagttat cagattctta tctacaaact
     1921 cttcatctac gaaaacaaat acctcacttg aatacaaccg tcttttctca agttcggatt
     1981 tgcgcattat agttcctgca ttacaaaagt atatctgcgg aagactatca gaaataagtt
     2041 tgtttttgag ttttcgcaac atctcttcgt aaacctctat tccatactca tagaagttta
     2101 ttccagcatc tatacagtac atctgtcttt tgcttataaa ctccataaca atgctaccgt
     2161 cggggggaga tttttcataa cgttcgtagc tctctatggt tctttgaaga gctcttttct
     2221 ggtacagcat ctcttttatc tgaaggtcta tctgattgct cttacgctct aaaatacttt
     2281 taaaaagaga tatatcgttc tgatccagaa gtttttttat atcaataagt ttcattccaa
     2341 gtgatttcat atactgtatc atatcaagac gagcacactg tctgatactg tagtaacggt
     2401 agccgttttc ggcatttatt tcatctggtt ttataagatc aagcttgtca tagagtctca
     2461 aagtttgttc ggaaatatga tttatctttg ccatcttgcc tattgtaagt ttttccatag
     2521 tccctccttg cctatataaa gtctatacat gcaataaggt ttcaatacac aaactgtatc
     2581 attctttgtt gtttcttatc cattcatcgg tcgctggaag taatatttgt tttccgtcat
     2641 ggaaggagag tatatacacg gttggttttt ctgaataggt tgccgtcaca aaagaaaaat
     2701 gaccggtgtt ggtggcagca ccccattgag cgtcgttatt catgcaaacc acagatatat
     2761 ctccggcttg acctcgtttt tccataagct ttttgttgaa gttttcaacc gctctttttg
     2821 cagcatcctg tggagataaa cctgattcca tcaaacgcac ggtttcataa cagacacagc
     2881 ctttcatgat atcctcacca aggcctgtag cggctgccga acctatattg ctatctgaat
     2941 aaagaccgca acccggtaag ggggaatcac cgattcttcc cgtttttttc ataaaaagac
     3001 cgctggttga agttgctgct gccatatgag atgatgtatc aatcccaact gcacctacgg
     3061 tatcatgacc gtcataaggt ttaagcgcat ctttcttttc aagaagtttt ttttgccata
     3121 tggcataaga agattcagtt atcatattct tttcttcaaa accatttatc ctggcatact
     3181 cctgcgcacc ttgaccagaa agaaagatgt tgaatctttc atccataagt tttctggcta
     3241 tttttacagg gttcttgaaa ttgacaattc cactcacggc tccgaaagat aaagtagttc
     3301 catccataaa agctgcatca agttctataa tacctttttc gttgggaaga gctccgtatc
     3361 ctacggaagt atagtccgga cagtcctcca cctctgttat tacctgttgt aaggcgtcga
     3421 agatggaact cccttgatca agcattaacc cggctttttt aatgccatct aaagccattc
     3481 tccatgtagc tatgactccc catttcataa aatacctcca aaaaattatc tgtttatatt
     3541 ttcaatggct ctacttacta tatattcgtc tgcaatataa tgctcaaact ctgatagtga
     3601 atgaggactc cccatagcaa cgctttcatt tctaaaaatc atatcgctgt ttttaatagt
     3661 ttttccagag agatgaacct cggttgcacc ggtggcggta ataacagctt ttatattttt
     3721 ttcatttata cctgcgccgg gcataataat tagttcatta ccaaaaatat ttattagatc
     3781 agagatcatt tttgatcctt gcacggcaga aggcttctgt ccggatgtaa gaatcctctc
     3841 gatacccata tcctttaaga cagcagcaca ggcatatgga tctttgagca tatcaaatgc
     3901 tctgtgaaaa cttattttta aagggtatgc agcttccaaa agagtctgta gcttctttat
     3961 gtcaactgta ccctgtggag taagtacgcc gcatactata ccgttaacac cgacggattt
     4021 tgctatttct atatctctgc acattatctg tatttcatca tcagagtaaa gaaaatcgcc
     4081 gcttctgggt cgtatgagca catgaatagg tatagttact ttctttttgg tatactccat
     4141 taatgcagca ctcggagtca gacctccgca agacagagca ctgcacagtt caattctctg
     4201 agctcctgct ttctgagcaa tctgtgctga ctctaaagaa tcagcacaga cttctaccag
     4261 gataggcttc atacattttt cttttctaaa ataacaacag tttctacatg ataagtgttt
     4321 gggaacatgt caaaaggctg aacagaaagt acttcaaaac cgctttccat aaatgctctt
     4381 atatctcgcg ccagagttgt tggatcacat gatatatata ctatggcctt ttcagttatt
     4441 tttgcaagat ttttagctga aagaacacct attccttctc taggaggatc gattatagcg
     4501 taagagaatt tttttgattc aatacacgat tgaagaataa aatcttctga ttttgaggac
     4561 gaaaagcgtg tattccttac cgagtttata tttgaatttg aacaggcagc ttttatagaa
     4621 acctttgagg tctctacacc gtaaacggtc ttgaaaagag gagcaaaata aatactgaaa
     4681 agccccacac cacaataaag atcaagcagt gaatctgatg cggtgtttaa agttttgaag
     4741 tatttgagaa tgtagttaag cattttatga gtaacgttat agttattctg aaaaaaagaa
     4801 gtaggcggaa tctgaaataa aaatccgtca aactcttctc ttaaaactcc ttcacccttc
     4861 catgttttgt atggtcctct taaggtaact gtgtcggaag tattcatcac atgaactata
     4921 gaatt
//
LOCUS       DAOSHR010000150         4880 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_15455_length_4880_cov_6.197306, whole genome shotgun sequence.
ACCESSION   DAOSHR010000150 DAOSHR010000000
VERSION     DAOSHR010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 4880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4880
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15455_length_4880_cov_6.197306"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /gene="whiA"
                     /locus_tag="PLU28_08255"
     CDS             <1..684
                     /gene="whiA"
                     /locus_tag="PLU28_08255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HOG35088.1"
                     /translation="RIGRMGEVLLNLEYVDKVLSGEGISIFEDSVPEAIKNDPVKFGV
                     FLKGMFLTTGSISMKSSYHMEFFTDISENYADDLIKVFQTLAGVKANYLVKNNKIKLY
                     IKSSQDIINVLELMQAHRTIEKLQETLRVRYLKGSVSRTINFISANAEKTAQSSSNQI
                     NDINYIKQTVGLNSLDEKSQIIAQYRLENEEDSLKDMADALGMSKSTLYNKLKKISEI
                     AQEIRKNKE"
     gene            708..1157
                     /gene="nrdR"
                     /locus_tag="PLU28_08260"
     CDS             708..1157
                     /gene="nrdR"
                     /locus_tag="PLU28_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HOG35089.1"
                     /translation="MKCPFCGYEDSKVLDSRPVGDGVSIRRRRECISCSGRFTTYERY
                     EDNKILVVKKDGKREQFDSKKVMNGIIKACEKRPVTYEQIEDMVAKIEDKLRKSGKIE
                     VQSKDIGDMIMEKLKIADQVAYVRFASVYREFRDIDQFLAVIRELKN"
     gene            1181..1681
                     /gene="greA"
                     /locus_tag="PLU28_08265"
     CDS             1181..1681
                     /gene="greA"
                     /locus_tag="PLU28_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOG35090.1"
                     /translation="MSDTVVKLTKEGYEKLKKERDDLKEKLMGEIAYRIKEARELGDL
                     SENSEYEEAKNEQGKIDSRIKEIEYILDHAEIIEEEEDSSVVGIGKRIKIRDMALEKE
                     KQEKIITLVTSQEANIREDKISEESPIGKAIKGKKVGETITVKTNSGSKKIKIVQIIN
                     EDAGKR"
     gene            1719..3230
                     /gene="lysS"
                     /locus_tag="PLU28_08270"
     CDS             1719..3230
                     /gene="lysS"
                     /locus_tag="PLU28_08270"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOG35091.1"
                     /translation="MDIRQQRIDAINELREKGINPFPYSFKKDMSSEQIQGKYDGVIQ
                     PEQMIEADKISFAGRIMTIRHHGKSAFLTIKDDTGRIQAYIRLDRVGAEKYEIFKKYV
                     DLGDWIGIEGFPFKTKTGELTILVENFELLSKSIRPIPEKWHGLKDREVKYRQRYVDM
                     ISNDDVIKTFRTRFEAIRLVREFMHSRNFVEVETPILESIMGGANARPFITHLNVFDA
                     DMYLRIAQELHLKRLVVGGMERVFELGRNFRNEGVSFKHNPEFTTIEIYQAYADYSDM
                     MELTENLLAYLAQNIHHSNKIVYDGTEIDFTPPFRRVDMRDFIKENLGVDILEDSDEK
                     IQQYLKGQNALPEILDKSHYIDALWDMVESKLIQPTFVMHHPVEISPLAKRHRKDPRV
                     TERFELIVYGREIANAFSELNDAVDQYERFKKQADLKEKGDDEAQMMDKDFVRALEYG
                     LPPTGGLGIGIDRVAMFLTNSPTIRDVIAFPIVKPIPFEDEEKDFEEEQGSNE"
     gene            3339..3530
                     /gene="rpmB"
                     /locus_tag="PLU28_08275"
     CDS             3339..3530
                     /gene="rpmB"
                     /locus_tag="PLU28_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOG35092.1"
                     /translation="MSKRCEICGKEPRGGNTVAHSKVTTRRWWKPNIQRVKVVLDDGT
                     VKRIHVCTSCLSAGKVNRG"
     gene            complement(3679..3756)
                     /locus_tag="PLU28_08280"
     tRNA            complement(3679..3756)
                     /locus_tag="PLU28_08280"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3719..3721),aa:Ile,seq:cat)
     gene            3859..4044
                     /locus_tag="PLU28_08285"
     CDS             3859..4044
                     /locus_tag="PLU28_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35093.1"
                     /translation="MLEGDIIKIAQIDMSDPVKLSINKYPRILVIRAAFEVLKAGKKI
                     TRKTLEKELIRNVKVIG"
     gene            complement(4065..>4880)
                     /locus_tag="PLU28_08290"
     CDS             complement(4065..>4880)
                     /locus_tag="PLU28_08290"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HOG35094.1"
                     /translation="ASVSMLNTVIDTSIATWTGDSGVSIISYALRIYQLPLSLFSVSV
                     ANSLLPKLSKAVIEKDKTSYSKNLRDSVNIILFFTVPSCIALLFLNRDIISLIFEHGS
                     FSSQDSIAVGAVLAAYAIGLPFYSLHTIFIRTYHSKLNTKYPMYVAVVLLAVNAVLDM
                     LFAKSLGPVGIALATSFSGIVGCILTGFKSLKYFSSKDFAEMGKILLASLCMLSTMTL
                     NLFISDSFFLKLIKIILSCIIFFAVAYFLKVRQLNSAITLINSKFRQKHSSSK"
BASE COUNT         1777 a          762 c         1087 g         1254 t
ORIGIN      
        1 cgcataggga gaatgggaga ggtactcctc aatcttgagt atgtcgacaa ggttctttcc
       61 ggagaaggaa tttctatatt tgaagattcc gttcccgaag ctataaaaaa tgatcctgtt
      121 aagtttggag tttttcttaa ggggatgttt cttacaacag gatcaatatc tatgaagtcg
      181 tcctaccata tggaattttt tacagatata agcgagaact atgcggacga tctgataaag
      241 gttttccaaa ctcttgcggg agttaaggcc aactatcttg taaagaacaa caaaataaag
      301 ctatatataa aatcaagtca ggatataata aatgttctgg aactgatgca ggctcacagg
      361 acgattgaaa agctccagga aactttaagg gtaaggtatc tgaagggaag tgtcagccgt
      421 accataaact ttatcagtgc caatgctgag aaaacagctc agagctcttc taatcaaata
      481 aacgatataa actacataaa acagactgtc gggctgaact cgcttgacga gaaatcgcaa
      541 attattgctc agtacaggct tgaaaatgaa gaagacagtc tcaaggatat ggcggatgcg
      601 cttggaatgt caaaatcaac tttatataac aagttgaaaa agatatctga aattgctcag
      661 gaaataagaa agaataaaga ataattcttt gcttgtgggg tgaaagtatg aagtgtcctt
      721 tttgcggata cgaagactct aaagttcttg actcaagacc cgtaggtgac ggagtatcaa
      781 taaggcgcag gagagaatgt ataagctgtt cgggaagatt cacaacatat gaaaggtatg
      841 aagacaacaa gatactggtg gttaaaaaag acggaaaaag agagcagttt gactcgaaaa
      901 aggtgatgaa cggaattata aaagcttgtg aaaaaagacc ggttacatat gaacagatag
      961 aggatatggt tgctaagata gaggataagc taagaaaatc cggaaaaatt gaagttcaat
     1021 ccaaggatat tggcgatatg attatggaaa aactaaaaat tgctgatcag gttgcttatg
     1081 tgcgttttgc ctctgtttac agagagttca gggacataga tcagtttctt gctgttatta
     1141 gagagttgaa aaattaacca gtctttaagg aggtacaagt atgagtgata cagttgttaa
     1201 acttactaag gaagggtatg aaaagctgaa aaaagaaaga gacgatctaa aagaaaaact
     1261 aatgggagag atagcctaca gaataaaaga ggcaagggag ctcggagatc tttctgaaaa
     1321 cagcgagtat gaagaggcta aaaacgagca gggaaagatt gattccagaa taaaagaaat
     1381 agagtatata ctggatcatg ctgaaataat agaagaggaa gaagactctt ccgtcgtagg
     1441 aatcggtaaa agaataaaga taagggatat ggctctggag aaagaaaagc aggaaaaaat
     1501 aattaccctt gtaacctcac aggaagccaa tataagagaa gataagataa gtgaagagtc
     1561 tcctatagga aaagccataa aaggcaaaaa ggtaggagaa actataaccg taaaaaccaa
     1621 ttcaggcagc aagaaaataa agatagttca gattataaat gaggatgcag gaaaaaggtg
     1681 aacagataaa ctgataataa ccggaggtgt tttctaatat ggatatcagg caacaacgta
     1741 tagatgcaat aaacgagctt agagaaaagg ggataaatcc gtttccctac agttttaaaa
     1801 aagatatgtc cagtgagcag atacaaggaa aatatgacgg agttatccag cctgagcaga
     1861 tgatagaagc tgacaaaata tcttttgccg gaagaatcat gacgataagg catcatggga
     1921 agtctgcatt tttaaccata aaggatgaca caggaagaat tcaggcttac ataagattgg
     1981 acagagtcgg agcagaaaag tatgaaattt ttaagaagta tgttgatctt ggtgactgga
     2041 ttggaataga aggttttcct tttaaaacca aaacaggaga acttaccata cttgttgaaa
     2101 actttgaact gctttccaaa tcaataagac cgattcctga aaaatggcat ggacttaagg
     2161 acagagaagt aaagtacaga cagagatatg ttgatatgat ttccaacgat gatgtgataa
     2221 aaaccttccg taccaggttc gaagctataa gacttgtaag ggagtttatg cactctagaa
     2281 actttgttga ggttgaaacg cctatactgg aaagcataat gggtggtgcc aatgcaaggc
     2341 cgtttattac ccatctcaat gtttttgatg ctgatatgta tctaagaata gcccaagaac
     2401 ttcatttgaa aaggcttgtg gtcggaggaa tggagagagt ttttgagctt ggcaggaact
     2461 tcaggaatga aggagtctct tttaagcata atcccgaatt tacaacaata gagatatatc
     2521 aggcatatgc agattattcc gatatgatgg agcttaccga aaatcttctt gcatatcttg
     2581 ctcagaatat acatcacagt aataagattg tatatgacgg cactgaaatt gattttacac
     2641 caccattcag aagagtagat atgagggatt tcataaagga aaacctgggt gtcgacattc
     2701 ttgaggacag cgacgaaaag atacaacagt atctcaaggg tcagaatgct cttccggaaa
     2761 ttcttgataa gtctcactat attgatgctc tttgggatat ggtagaaagc aagcttatac
     2821 agcctacctt tgttatgcac catcccgttg agatttctcc tcttgccaag aggcacagaa
     2881 aagatccgag agtaaccgaa agatttgagc tcattgtata tggaagagag attgctaatg
     2941 cattcagcga gctgaacgat gcagttgacc agtacgaaag atttaaaaaa caggctgatc
     3001 ttaaagaaaa gggtgatgat gaagcccaga tgatggataa agattttgtc agagctcttg
     3061 agtatggact tcctcctaca ggaggacttg gtataggcat agacagagtt gccatgtttc
     3121 ttaccaactc tcctacgata agagatgtca tagccttccc gatagtaaaa cctattccgt
     3181 ttgaagatga agaaaaggat tttgaagagg aacagggaag caacgaataa ttctgaatta
     3241 aaaattaact gaaagtaact aattgtaatc taagaacttc tttgttgtat aatagataat
     3301 gcgtataaaa tcaatagctg ttgagaggag gacataaaat gtcaaaaaga tgcgagatct
     3361 gtggcaaaga acccagaggc ggcaatacgg ttgctcactc caaggttacc acaagaagat
     3421 ggtggaaacc caatatacag agagtaaagg ttgttcttga cgacggtacg gtaaaaagaa
     3481 tacacgtatg cacatcctgt cttagcgcag gaaaagtaaa cagaggctaa aaccaaaatg
     3541 tgaccggtcc ttactgagga ccggtttttt tatgctaaaa tcatggtata ttaatttttt
     3601 ttgaaaatat tgacattata aggtaaaaat attaaaattg aatcaagatg aaaaaaaccg
     3661 ggattacccc ggttaatttg gtgggctcgg atggagtcga accatcgacc gcccggttat
     3721 gagccggaag ctctaaccaa actgagctac gagcccgaac gaccgaaatc aattatatca
     3781 tcagaatagc tatttgtcaa cagtcttact ttaagaatta aataagagaa ttttaaaata
     3841 aagatggggg ggagtcttgt gttggaagga gacataatca aaatagccca gatagatatg
     3901 tctgaccctg taaagctcag cataaacaag tacccgagga tactagtcat aagagctgct
     3961 tttgaagttc tcaaggccgg caaaaaaatc acacgtaaaa cccttgaaaa agaacttatt
     4021 agaaatgtaa aagttatagg ataaaaaacc cctgcagggg ttttttattt tgatgagctg
     4081 tgtttttgtc taaatttgct attaattaat gtgattgctg aattgagctg tctaaccttt
     4141 agaaagtaag caactgcgaa gaagatgata cagctaagaa taattttgat cagttttaga
     4201 aaaaaagaat cagaaatgaa aagattcagt gtcatagtag agagcatgca taaagaagcc
     4261 agtagtattt tgcccatctc ggcaaagtct tttgatgaaa aatacttcag agacttaaaa
     4321 cctgtaagga tacagcctac aattcccgaa aacgatgtag ctaaagctat tcccacagga
     4381 cccaagcttt ttgcaaaaag catatcaaga accgcattta cagccagaag aactaccgca
     4441 acatacatcg gatactttgt gttgagcttt gaatgatacg tgcgtataaa aatcgtatgc
     4501 agagagtaaa aaggaagccc tatggcatag gctgcaagaa ctgcaccaac ggctatactg
     4561 tcttgtgagc tgaagcttcc gtgttcaaat atcaatgaaa ttatatccct atttagaaac
     4621 aaaagtgcga tacatgaagg aaccgtaaaa aagagtatta tgtttacgct gtcacgaaga
     4681 ttttttgagt acgatgtttt gtctttttct ataactgctt ttgataattt gggaagtaat
     4741 gaatttgcaa cactcacaga aaagagagaa agcggtagct gatatattct cagtgcatag
     4801 gaaattattg aaacgccgct gtcacctgtc catgtagcaa tgctggtatc tattaccgta
     4861 ttgagcatag aaacagatgc
//
LOCUS       DAOSHR010000151         4876 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_15476_length_4876_cov_4.821614, whole genome shotgun sequence.
ACCESSION   DAOSHR010000151 DAOSHR010000000
VERSION     DAOSHR010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 4876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4876
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15476_length_4876_cov_4.821614"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3241)
                     /locus_tag="PLU28_08295"
     CDS             complement(<1..3241)
                     /locus_tag="PLU28_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOG35095.1"
                     /translation="MAKVSSFQRKISKIKVGLSSPERILSWSNGEVKKPETLNHRTSK
                     PEKDGLFCERIFGPMKDYECACGKYKGKKYEGTVCERCGVRVEPKEARRRRMGHIELA
                     TPVGHIWYLKSSPSIMSITLGIPVKDLENIIYYGSKRVMERVFLTLANQDKDTESELD
                     TYQPGDVLYQTEYDIYSNYMEIPVEHAVRIAKVKAQAVSEIEGTVHIETETTQTEREL
                     NWIIIKSDEGQIKRYPVFEGASLLVEEGQKIEAGVQLADRVLFEDDYLTQEEISIFTE
                     YYPGSVELEKDIERDTPIVVITDIDKDFSKRIGKTTGDILLEDEAFAYEEVMRILNQR
                     VSADREGIVDKILSQEVRTEEMFYEKGMQITDEIVDSMVSAGIKHIYAQDDTNLEKLY
                     LINEYEGFEAKYGSEAVKELLCNIDLEVLKAKLEADISKLDKKSQKAIKLLKRLKIIK
                     DFIKSSNKPEWMIMENLPVVPPDLRPLIQIDGGRFAATDLNDLYRKVINRNNRLKKLL
                     EMEAPDIIVRNEKRILQQAVDSLIYNGRVGKPITDRSKRPLRSLTDLLKGKKGRFRRN
                     LLGKRVDYSGRAVITVGPDLQIHECGLPKKMALELFKPFVLAELLKDSNVASKNARKL
                     KKTIIEKEMPQAWEILEEVIKGHPVMLNRAPTLHRMSIQAFIPKLIEGNAIRLHPLVC
                     TPFNADFDGDQMAVHVPLSAVSQAEAKFLMLSRYNIVSPANGKPISMPGKDIIVGAYY
                     LTTHDNKLFDESKIPQKVTDIGKDNNVRFCFSDSMHAVYAFEYLTVVTSGVFTHNNKL
                     NWKGTYYNEGGLGERIILSLHDPIVFRDENQKLIKTTVGKIIFNEVVPPELRDYKAKM
                     DKKGLRNVIDKTFKKYGIDRTADLLDDIKYFGFHYATVSGLTISIRDVIVAERKNEIV
                     ADSENKVLEIEKLYEEGYLTDHERYKTIVENWEETTKKVTEVTGEAYKNKTYNPIWIM
                     LDSGARGSIDQLKQLAGMRGLMADPSGRTIEVPIKSNFKMGLSELEFFTSTHGSRKGS
                     ADTALRTSTAGYLTRRLVDVAQSIVVMETDCGTSKGIEAEELWSDNSKVE"
     gene            complement(3304..>4876)
                     /locus_tag="PLU28_08300"
     CDS             complement(3304..>4876)
                     /locus_tag="PLU28_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOG35096.1"
                     /translation="GVEWLAARDSGYLIFAKHRGVIEHVDGRKIVIARTLDEGEDCRT
                     KEKDGKRYLVDNEGNIIKDEYQILKYIRSNQDMCINQRPVVSKDQTVEKGDILADGPA
                     TDMGELALGKNVLVGFLPWEGYNFEDAILVSEELLERDTFTSVHIEVYETKAMDTQLG
                     PEEITADIPNVKKELLKNLNEEGIVRIGAYVSSGDILVGKVTPRGESETSPEEKLIKS
                     VFGDRGRDVKDTSLTLPHGVEGRVIDVQKFTREDIPGLEIGVNTYVKIYIAAKKTLEA
                     GDKLAGRHGNKGVVSKILHKEDMPFMPDGRPLQMVLSPLGVPSRMNIGQVLELHLGWL
                     ADLSGKFYATPIFDGATEEEIMSELHKLRKEKKLDLGERDEDVTGKIILRDGRSGKPF
                     DFPVSIGYMYMLRLSHIAKDKIHARATGPYSLIHQQPLGGKAHFGGQRFGEMEVWALE
                     AYGAAHTLTEMLTYKSDDIRGRNEVYKAVLKGENLPEPGIPESFKVLVKELQGLALDV
                     KLFDEDGNELDVDKI"
BASE COUNT         1159 a         1089 c          915 g         1713 t
ORIGIN      
        1 tttcaacttt ggagttgtct gaccaaagtt cttccgcttc tatgcctttg gatgtaccac
       61 agtctgtttc cattacaact atcgactgtg cgacatcaac cagtctcctt gtaagatatc
      121 ctgctgttga cgttcgcaga gcggtatcgg ctgagccttt tcttgatcca tgagtagatg
      181 tgaagaactc aagttcagaa agccccatct taaagtttga ttttatggga acctctatgg
      241 ttcttccgga aggatctgcc ataagacctc tcatacctgc gagctgcttt agctgatcta
      301 tgcttcctct tgctcctgaa tcaagcatta tccatatagg attataggtc ttgttcttgt
      361 atgcctcgcc tgtaacttcg gtaacttttt tggtggtttc ttcccagttt tcaacaatag
      421 ttttgtatct ttcgtgatcg gtaagatatc cttcttcgta aagtttttct atttcaagta
      481 ccttattttc agaatctgca acgatctcat tttttctttc ggcaactata acatcgcgta
      541 tggatatagt aagtcctgaa actgttgcat agtggaaacc aaagtacttt atatcgtcca
      601 aaagatctgc cgttctgtct attccgtact ttttgaaggt cttgtctata acgtttctta
      661 agcctttttt gtccatcttg gctttgtaat ccctgagctc tggaggaaca acttcattga
      721 agattatctt tccgacagtt gtttttatca atttctggtt ttcgtctctg aatactatag
      781 gatcatgaag ggataagatt attctttctc ccaaaccacc ctcgttatag taagttcctt
      841 tccagttcaa cttgttatta tgagtaaaaa caccgctggt taccacggta aggtattcaa
      901 aagcatatac cgcatgcatc gaatctgaaa agcagaatct tacattattg tctttcccta
      961 tatctgttac tttctgaggt atcttcgatt catcaaaaag tttattgtca tgtgtggtaa
     1021 ggtagtacgc accaactatt atatcttttc cgggcattga tataggtttc ccgtttgccg
     1081 gagagactat gttgtatcgt gaaagcataa ggaactttgc ttctgcctgt gatacggctg
     1141 aaagcggtac atgcacggcc atctggtctc cgtcgaagtc cgcgttaaaa ggtgtgcaga
     1201 cgagcgggtg gagtcttatg gcgtttcctt ctatgagctt gggaatgaat gcctgtattg
     1261 acattctgtg gagtgtagga gctctgttaa gcataacagg gtgtccttta ataacttctt
     1321 ccagaatttc ccatgcctga ggcatctctt tttcaattat ggtctttttg agttttcttg
     1381 cattcttgct tgcaacgttt gaatctttga gaagctcggc gagaacaaaa ggcttaaaaa
     1441 gctcaagagc catctttttg ggcagtccgc attcgtgtat ctggagatcc ggtcctacgg
     1501 taataacggc tcgtccggag taatcaactc tttttcccag aagattcctt ctgaaacggc
     1561 cttttttacc ttttagaaga tcagtaagcg atcttaaagg cctcttgctc ctgtcggtta
     1621 ttggttttcc gactctgcca ttgtatataa gcgaatctac agcctgctgg agaattctct
     1681 tttcatttct tactattata tctggcgctt ccatctccag gagctttttc agtctgttat
     1741 tcctgttgat gacctttctg tacagatcat tcaggtctgt tgcggcaaat cttcctccgt
     1801 caatctgtat aagaggcctg agatcgggcg gaacaacagg aagattttcc ataatcatcc
     1861 attcaggttt gttggaagat tttatgaaat ccttgattat tttaagtctt ttaagtaatt
     1921 tgattgcttt ctggcttttt ttgtcaagtt ttgatatatc agcttcgagc ttggccttca
     1981 gaacttcaag atcaatgttg catagaagtt ctttaaccgc ttccgaacca tacttggctt
     2041 caaaaccttc gtattcgttt ataaggtaaa gtttttcaag attggtgtca tcctgggcat
     2101 agatatgctt tataccggcg gataccattg agtcaacgat ctcatcagtt atttgcatcc
     2161 ctttttcata aaacatttct tcagttctga cttcctgtga aagtatcttg tccactatac
     2221 cttcacggtc ggcggagact ctctggttca gtatcctcat tacttcttcg taggcaaagg
     2281 cttcatcttc aagtagtata tctcccgtgg tctttccgat tctttttgaa aagtctttat
     2341 cgatatctgt tatgactact ataggagtat ctctttctat atccttttca agttcaaccg
     2401 agccgggata gtattctgta aatatggaga tttcttcctg agtcagatag tcatcttcga
     2461 agagaaccct gtctgcaagc tgtactccgg cttcaatctt ctgaccttcc tctacaagaa
     2521 gggaagcgcc ttcaaaaaca ggatatctct ttatctgacc ttcatcggac tttattatta
     2581 tccagttgag ttctctctcg gtctgtgtgg tctctgtctc tatgtgaacg gttccttcaa
     2641 tctctgaaac tgcctgcgct ttcactttgg ctattcttac ggcatgctct accggtattt
     2701 ccatgtagtt ggagtagata tcatactcag tctggtaaag aacatctcca ggctgatatg
     2761 tatcaagctc tgattcagta tccttgtcct ggtttgccaa agtaagaaat acccgttcca
     2821 tgactctttt tgatccgtaa tatattatat tttcgagatc ttttacaggt attccaagcg
     2881 taatagacat tatagaaggt gatgatttta aataccatat atgtcccaca ggggttgcaa
     2941 gctctatgtg acccatacgt cttcttctcg cttcttttgg ttcaacacgg actccgcatc
     3001 tttcacatac ggttccttcg tattttttac ctttatactt accgcaggca cactcataat
     3061 ctttcattgg gccgaagatt ctttcgcaga aaagtccgtc tttttctggt ttgcttgttc
     3121 tgtgattaag agtttcgggt tttttaactt ctccgtttga ccatgaaagg attctctcag
     3181 gagaagacaa accaactttt attttcgata tttttctctg aaatgaagat actttcgcca
     3241 ttaatcttcc ctccttgagg gtataattaa tttctttatt ctttttaaca gatgcctgtt
     3301 agcctaaatc ttatccacat caagttcgtt gccgtcttcg tcgaaaagtt tgacatcaag
     3361 ggcaagaccc tgaagttctt ttacaagaac cttaaagctt tcgggtatac ctggctcagg
     3421 aagattttca cctttgagca cagctttata aacttcgttt cttccacgta tatcgtcaga
     3481 cttataagta agcatttctg taagggtatg agcggcaccg taagcttcaa gcgcccatac
     3541 ttccatttct ccgaatctct gtccgccaaa gtgggctttt ccgcccagag gctgttgatg
     3601 aatgagagag tatggaccgg ttgcccttgc gtgtatctta tctttggcta tatgtgacag
     3661 cctgagcata tacatgtaac cgattgaaac cggaaaatca aaaggttttc cgcttcttcc
     3721 gtctctgagg attatctttc cggttacgtc ctcgtctctt tcgcctaggt caagcttttt
     3781 ctctttcctt aatttatgga gttcactcat tatttcttct tctgtagcac cgtcgaatat
     3841 gggcgttgcg taaaattttc cgcttaaatc agcaagccat ccgaggtgaa gctctaaaac
     3901 ctgtcctata ttcatccttg aaggaacgcc caatggtgat agaaccatct gcagaggtct
     3961 tccatcaggc ataaaaggca tgtcctcttt atgcagtatc ttggaaacaa cacctttgtt
     4021 tccatggcgt ccggcaagct tatctccggc ttccaaggtt tttttagctg caatatatat
     4081 ttttacgtat gtatttactc ctatttcaag tcctggaata tcttcacgtg taaatttctg
     4141 aacatcaatt actcttcctt caacaccgtg gggcagagta agagaggtat cttttacatc
     4201 gcgtcccctg tctccaaaaa ctgattttat aagtttttct tcaggcgatg tttccgattc
     4261 tccccttgga gtaacctttc ctacaaggat atctcccgat gatacatagg ctccgattcg
     4321 gacaatacct tcttcattga ggttttttag aagttccttt tttacattcg ggatatctgc
     4381 tgttatttct tcaggaccta gctgtgtatc catagccttg gtttcatata cttctatatg
     4441 taccgatgtg aaggtgtctc tttccaaaag ctcttcactt acaaggatgg cgtcttcaaa
     4501 gttgtatcct tcccagggca aaaagccgac aagaacattt ttgccgagtg caagttcacc
     4561 catatcggta gctggcccgt ctgcaagaat atctcccttt tcaacagtct gatctttgga
     4621 aacaacagga cgctgattta tacacatatc ctgatttgaa cgtatatatt ttaatatctg
     4681 gtactcatct tttattatat ttccttcatt gtcaacaaga taacgtttac cgtctttttc
     4741 ttttgtacgg cagtcttcac cttcgtcaag agttcgcgca atgactatct tcctgccgtc
     4801 gacatgttca attactcctc tgtgctttgc aaatataaga tatccggagt ctcttgccgc
     4861 cagccactca acgccg
//
LOCUS       DAOSHR010000152         4840 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_15631_length_4840_cov_5.340857, whole genome shotgun sequence.
ACCESSION   DAOSHR010000152 DAOSHR010000000
VERSION     DAOSHR010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 4840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4840
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15631_length_4840_cov_5.340857"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="PLU28_08305"
     CDS             complement(<1..375)
                     /locus_tag="PLU28_08305"
                     /inference="COORDINATES: protein motif:HMM:NF013486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HOG35097.1"
                     /translation="MNKIRFEKLFDLMEKNSTGQILVSSPSYIYYLTGVWPECGERMV
                     VLSISLNRSVKLIINRLFPIEPIEGVQLVYFDDSEPAVEVLCKNLDNSRTGIDKNWPS
                     RFLIELMDKKPEMSFFNSTFMLD"
     gene            complement(458..2149)
                     /locus_tag="PLU28_08310"
     CDS             complement(458..2149)
                     /locus_tag="PLU28_08310"
                     /inference="COORDINATES: protein motif:HMM:NF028259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35098.1"
                     /translation="MRARKLTSLTLTLINEHYGISKKWYTYAVKKQRLWEAALIGGAV
                     IALIASLVPLYSELLNPLMIESIKMGMGKLMLSNAFLYSSIFGLFLGMFTLISSFFFS
                     KETAALIPLPLKPAEVLSAKLFTVTIDQLLVSLFMLVPVIVLYGINSNMQIGYYITGL
                     IVFLFSQLFPMLLVSIIIFPLSSVVKFNKNKDFMIFFISTVVLVGVIFGQYYLNQFAM
                     NQSTAGLSSQDMAKLLANPDMFINKISMAYPPAFLALNALTQSDASSILWLLSYIGVH
                     LAAFLLVLWLAEKFYFSTYSELQENAAGKKKLSDNEFEKVFSVKSTMYRALMKREWRY
                     FLRVPAFSFNGLANVVIFPILLVVFAAGAYNIPEFQALMTFIDQYKELVVPVGSLLAA
                     LAGSMSLLPASAFSREGRLLIELKTLPVNPTAVIKVKFVHSNLISGVGVFCSAIAVGF
                     ITKIGWLEVLWIIVTGYLLTIFLNNLQMIVDTAKPMLEWDNPIKAMKQNINGLLAIAI
                     VFGFVFGLGYLEYITREYIPNQLMNPVLMLIGIAGSLITWKTLLIQAKKLFEKDL"
     gene            complement(2103..2864)
                     /locus_tag="PLU28_08315"
     CDS             complement(2103..2864)
                     /locus_tag="PLU28_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG35099.1"
                     /translation="MIELSELTKVYNGTVKAVDDISLTVKPGEIFGFLGPNGAGKSTS
                     IKMLAGILKPTSGSIKICGIDRMKDPIKAKMNIGYVPDEPLLMEKITGAEYINFIADV
                     FDVPPKIRTERAQKLLEYFKLINAVKDPISTYSHGMRQKLSLVCALIHNPPVWVLDEP
                     IVGLDPESAFILKKMMVSHAKSGNTVFFSTHIMEVAEKICDRIAIISKGKILFIGTVQ
                     EIRKAKGEQSLEKIFLEVTDSESEKIDFSYLDTNQ"
     gene            complement(2876..3625)
                     /locus_tag="PLU28_08320"
     CDS             complement(2876..3625)
                     /locus_tag="PLU28_08320"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOG35100.1"
                     /translation="MWIKEFADMRIRFFVILFIFSAALILFFGMKDYITQTILNVSLD
                     QGAFPLMSEEQSRLFMTNIHRFDFYTVSQWYGKNFGQFIPFIAIILAFPVFSREVEKK
                     TIYFLITRKKRSSIFFIKFFCGAVFNALIIFLMVTVPYLMSLFTDYPMRTDIFLNYLL
                     AQVSGGSFWYSITILFSVLFNDQIKTALASFGVFIAVSVLPLLEVLRFLNIYSYILNP
                     LQHTRLYGLVLLTVSAILCLGGNYIFKKKDY"
     gene            complement(3615..4463)
                     /locus_tag="PLU28_08325"
     CDS             complement(3615..4463)
                     /locus_tag="PLU28_08325"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG35101.1"
                     /translation="MALVQINSLKKSYRSGVRALKKISFDLQDNEVLALLGPNGAGKT
                     TALKSIMGIIKSGGEILFKSRPLTTEDALQNIVFVPEEKNLYDHFSAKQLSKIAKQLT
                     PKFDSSKFDCYMEVFELNKNQKFSHLSNGMKTLVYLSLAFSTDSLIYILDEPTWGLDP
                     LMRNTVLREIRNLTFNGKSVIYTSHILSEVEKTADRIVILNKGKITEFDSMDEIKLKY
                     AALSSSQDLPSYISKKRVSESDFLYIVRSSELAMLKNSYPLARQEEISFEDIFEALVR
                     RDSDVD"
     gene            complement(4435..>4840)
                     /locus_tag="PLU28_08330"
     CDS             complement(4435..>4840)
                     /locus_tag="PLU28_08330"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOG35102.1"
                     /translation="LIVWFKVDYRSSKPVYEQIKERIKEQILTKKLNPGEYLPSIRTL
                     AQDIGVNLNTVSRSYRELEAEGFVLSEKGLGYTVKTVQDDKIETEIMDEFAKYVKKLK
                     RSGIPQNKAIETIKNIYGNGGEESGTCPDKQS"
BASE COUNT         1475 a          967 c          901 g         1497 t
ORIGIN      
        1 gtcaagcatg aacgtagagt tgaaaaagct catttccggt tttttgtcca taagttcgat
       61 aagaaacctt gaaggccagt ttttgtctat ccctgttctt gagttatcaa ggttcttaca
      121 cagaacttca actgcaggtt cagaatcatc aaagtaaacc agttggactc cttctatagg
      181 ttcaatcgga aataatctgt tgataataag ttttacactg cggttcagac ttatgctcag
      241 aactaccatt ctttcgccgc actccggcca tactcctgta agatagtaaa tataagaagg
      301 agaagaaact aaaatttgac ctgtggaatt tttttccata agatcaaaaa gtttttcaaa
      361 acgtattttg ttcataataa acctcccctg tataaatgct ttcagtagta ttataccctt
      421 aaaaagacag aaaaaaacag cggaaaagtc cgctgtttca taaatctttt tcaaaaagct
      481 ttttggcctg tataagcaaa gttttccagg taattaggct tccagctatt cctataagca
      541 taagaaccgg attcatcagc tgatttggga tatactctct tgtaatgtat tcaaggtagc
      601 ctaagccaaa gacaaatcca aaaacaattg caattgcaag taatccattt atattctgtt
      661 tcatggcctt tataggattg tcccactcca gcatgggctt tgccgtatcg acaatcatct
      721 gtaggttatt caggaatatg gttaggagat atcccgttac tataatccaa agaacctcaa
      781 gccatcctat ttttgtgata aatcctactg caatggctga gcagaaaaca cctacaccgc
      841 ttatcaaatt agaatgcacg aactttactt ttattacagc cgttggattc acaggcagcg
      901 ttttcagttc tattaggagc cttccctcac ggctgaaggc tgatgcggga agaaggctca
      961 ttgatcctgc aagagcagcc agaagcgatc ccactggtac tacaagctct ttgtactgat
     1021 ctataaatgt cataagtgcc tgaaattcag gtatgttata agcacctgct gcaaaaacta
     1081 caagcagaat aggaaaaatt accacattag cgagtccgtt gaaactgaaa gccggtactc
     1141 tgagaaagta tctccattca cgtttcataa gggcacggta catagtactt ttgacagaaa
     1201 aaactttttc aaactcatta tcagaaagtt ttttctttcc ggcagcattc tcctgaagtt
     1261 ctgagtaggt tgaaaagtag aatttttcag caagccataa aaccagtaaa aaggctgcaa
     1321 gatgaactcc tatataactc aggagccaca aaatgcttga tgcgtcgctc tgagtaagcg
     1381 cattaagggc aagaaaagcc ggaggatacg ccatagaaat tttatttatg aacatatcag
     1441 ggtttgcaag caatttagcc atatcctgtg aagaaaggcc tgcagtgctt tgattcatgg
     1501 caaactggtt cagatagtac tggccgaaga taactcccac aagaactacg gtgcttatga
     1561 aaaatatcat aaaatcctta tttttattga acttaactac agaggagagc ggaaaaataa
     1621 ttatacttac cagcagcatg gggaaaagct gcgaaaaaag gaatactata agccctgtta
     1681 tataatagcc tatctgcata tttgagttga tcccgtacag aacaataaca ggaacaagca
     1741 taaacaaaga gactaagagt tgatctatcg ttactgtgaa aagttttgcg cttaggacct
     1801 cagccggttt taaaggcaga ggaatcaacg ctgccgtttc ttttgagaaa aagaaggagc
     1861 ttattaatgt aaacattcca agaaaaagtc cgaagatcga tgagtaaaga aaagcgtttg
     1921 aaagcataag tttccccatc cccattttaa tgctttcaat catcaatggg ttaagaagct
     1981 cactgtaaag tggtactaaa cttgctataa gagcaatgac cgctccacct ataagagctg
     2041 cttcccataa acgctgtttt ttaaccgcat aggtgtacca tttttttgat attccgtaat
     2101 gttcattgat tagtgtcaag gtaagagaag tcaattttct cgctctcact gtccgtcacc
     2161 tccaggaata tcttttcaag actctgttct cctttggcct ttcttatctc ttggactgtt
     2221 ccgatgaaaa gaatttttcc tttgcttata atggctatcc tgtcacatat tttttcggca
     2281 acttccataa tgtgagttga aaaaaataca gtattgcctg atttggcatg gcttaccatc
     2341 atttttttaa gaataaatgc cgattccggg tcaagaccaa caataggttc atcaagaacc
     2401 catacaggcg gattgtgtat caatgcgcag acaagcgaaa gcttctggcg cattccgtgc
     2461 gaataggttg atatagggtc tttcacagca ttgataagtt taaagtattc caggagcttc
     2521 tgtgctcttt ctgttcttat ttttggcggt acatcaaaga catcggctat aaagtttata
     2581 tactctgctc cggtgatttt ttccataagc aaaggttcgt caggaacata acctatattc
     2641 attttagcct ttatgggatc tttcattctg tcgattccac atatttttat acttcccgaa
     2701 gttggtttga gaattccggc aagcattttg atgcttgtgg actttccggc tccgtttggt
     2761 ccgagaaaac cgaaaatttc accgggtttt actgtcaaag atatgtcatc aacagctttc
     2821 acagttccgt tatatacttt agtcagttcg gataattcta tcatgtgctg cctcctcaat
     2881 agtctttttt cttgaatata tagtttccgc ctagacatag tatggctgat acggttaaaa
     2941 gaaccaatcc gtaaagtctt gtatgctgca gaggattaag aatataagag tagatgttca
     3001 aaaatcttag tacttcaagc aacggtaaaa ccgaaacggc tataaaaact ccgaaagagg
     3061 ccagggcagt ttttatctga tcattaaaga gtactgaaaa taagattgtt atactgtacc
     3121 agaaagatcc gccggatacc tgtgccagaa gataattcag aaatatatcg gttctcatgg
     3181 ggtaatctgt aaaaagtgac ataagataag gaacagttac catgagaaag atgattaatg
     3241 cattgaaaac agctccgcaa aaaaatttta taaaaaaaat gctgcttcgt ttttttcttg
     3301 ttatcagaaa gtatatggtt tttttctcca cttctcttga aaaaacagga aatgcaagaa
     3361 taatagcaat aaaaggtata aactgaccga aattcttgcc gtaccattga cttacggtat
     3421 aaaaatcgaa tctgtgtata ttggtcataa aaagtctgga ctgttcttct gacatgaggg
     3481 gaaaagctcc ctggtcaagc gatacgttta atatggtttg agtaatgtaa tccttcatgc
     3541 caaaaaaaag tataagtgct gcagagaaga tgaaaagtat aacgaaaaac cttattcgca
     3601 tatcagcaaa ctctttaatc cacatcagaa tccctccgta ccagcgcttc aaaaatatct
     3661 tcaaagctta tctcttcctg tcttgccaaa ggatacgagt ttttaagcat cgcaagctca
     3721 ctgctgcgca cgatatataa aaaatcgctc tctgaaaccc tcttcttgga aatataactg
     3781 ggaagatcct gtgaagatga aagtgccgca tacttaagct ttatctcatc catgctgtca
     3841 aactctgtta ttttgccttt attcagtatt acaattctgt cagcggtttt ttctacttcc
     3901 gaaagaatat ggcttgtata tatcacactt ttaccgttga aggtaagatt ccgtatttca
     3961 cgcagtactg tattgcgcat taacggatca agcccccatg tcggttcgtc cagaatatag
     4021 atcaacgaat cggtggaaaa tgccaaagat agataaacca aggttttcat accgtttgaa
     4081 agatgagaga acttctggtt cttattcagt tcaaatactt ccatatagca atcaaatttt
     4141 gatgagtcaa acttaggagt aagctgttta gctatctttg ataactgctt tgcagagaag
     4201 tgatcataaa gatttttttc ctccggcaca aaaacaatgt tctgcaaagc atcttccgtg
     4261 gtcagagggc gtgacttgaa aagaatttcc ccgccacttt tgattatacc catgattgac
     4321 ttgagagcag ttgtttttcc ggcaccgttt gggcccagca gtgcaagaac ctcgttatcc
     4381 tgcaggtcga acgatatttt ttttagtgcc ctgacccctg acctgtatga ttttttaaga
     4441 ctgtttatct ggacaagtgc cactttcctc accaccgttt ccgtatatgt ttttaattgt
     4501 ttcaattgct ttgttttgcg gtatcccgct tcttttaagt ttttttacat actttgcgaa
     4561 ctcatccatt atttctgttt ctatcttatc gtcctgaaca gtttttacag tgtatcccag
     4621 acctttttct gaaagtacaa aaccttctgc ttccagctct ctgtaggatc tggacacagt
     4681 gttaagatta acccctatat cctgcgccag agtccgtatt gagggaaggt actccccggg
     4741 atttaatttt tttgtaagga tctgttcctt tattctttcc tttatctgtt catagacagg
     4801 cttggaagat ctgtaatcca ccttgaacca cactattagc
//
LOCUS       DAOSHR010000153         4787 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_15881_length_4787_cov_4.446746, whole genome shotgun sequence.
ACCESSION   DAOSHR010000153 DAOSHR010000000
VERSION     DAOSHR010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 4787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4787
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15881_length_4787_cov_4.446746"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1343)
                     /locus_tag="PLU28_08335"
     CDS             complement(<1..1343)
                     /locus_tag="PLU28_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG35103.1"
                     /translation="MKMKDLKFWLKDFWSEYRKVKFGIVGFVFLIIFLLLMIFEPYIV
                     PFSQASSRWRDITYWDDNPSGAPPVWTNWFSSKKSPESEKITKYQSEVIEGDLKFVNS
                     TFEYDYKYDKAPLDIIFHGRGTGNMIMIVKMERPDGETFDLVQMPIQTDGGNDIRITL
                     DKDSGAAAYDFAQKFENEENLQYVDKTSVKPLDIYFAQAKSDMLNKIEPLKGIYKFTV
                     TTILLDQESSYEDPYMVVSGRVSGILGTDNSKRDIWSGVVAGVKWALLIGLLTAVIAV
                     GIGVVYGVISAYYGGFVDSIMQRIFELFASTPLLPVLIVMSAVFKPSIWTMILMMCAF
                     FWTGPVKTVRSMALQIKEETYIEASKAVGASNARLIFKHMVPILVPYSFASMALYVPS
                     AIVYEATISLIGLGDATIVTWGQILHDALKGGAVLTGLWWWVVPPGIMIALMGMT"
     gene            complement(1340..2380)
                     /locus_tag="PLU28_08340"
     CDS             complement(1340..2380)
                     /locus_tag="PLU28_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG35104.1"
                     /translation="MYWKYAAKRIFYGIVMYIILIFIFSVLFNATNEATTKAQIEEAL
                     RAETTKMRNMDVQEVQKYIAERRAYKYHLYHLDQPIFQRIVWRTIDTITLNFGNSTNI
                     KSAKGERDVWTIVSETIPRTILLFTTAMILELILGVWLGLKKAQRAGGVLDKSTSIAT
                     MVVYGMPRWWLGMIMIMFFAYQVNIFPSGGLHTVPPPQGFAYFLDILYHMALPMLTLL
                     IIDFWGTAYLTRNIVLGTLQEDFVMSARARGIPENKVLFGHTMRTSAPPIVTMALLSL
                     LSSVSGNILFEGIFSWPGMGNLYWIAIEQNDVPVLMGLLAITTALYIAGLVILDLIYG
                     FLDPRIKVGGKA"
     gene            complement(2530..>4787)
                     /locus_tag="PLU28_08345"
     CDS             complement(2530..>4787)
                     /locus_tag="PLU28_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35105.1"
                     /translation="MVNPIPNAAPYTWTVKSGETFFNPLAMKEIRFALNFLISRKYLV
                     DEILGGAGGAMFTPMTPGQPGTYRYNLIATKYGMTAQGNEKKAITDINAAMEKAAALA
                     ENKGKLVKKDGWWMYNNKPVTIKFLIRVDDPQGRLKAGRYIADQIEKSGIKVERLEYD
                     RSKCVKLAYNGDPADYEWGLYTEGWGAGATRAFWDITIAQMYSPWYGYMAGGAVPEFW
                     NYKQNEIDALSQKIFNGLFLTEEEYWTDNLRATELGLEDSARIYLAYQDQYFAANKDR
                     FNNRVVYGLGDGLNRWAHITADVKPDSNGQKVLRLTQYSAKGSLFMSAWDPIGVDGFS
                     DTYSQVISDPLYDSSYFEAPNSAKDTAYRAFGKDVVTKVEMKGDKLVGTLQVPADAIK
                     YNSMTKKWEKVGPGVTSFSKATYSFKFGKWHSGRPIGIADIMYVQAFLVEWMNKDGAN
                     DKYYEPTYESLARSQMETFKGYVVNRDNTITVYFDFNFPLDPDRVAATGAPWLKLTPA
                     GVNVSVSWEIAEAMAKLVAEGSKSGTVYSFSSDPAFTEIDALSPKCVADIKAKLEEMK
                     SAKYVPASVKDYMTAADAVSNYEAAIKFIDTYGHAYISNGPFYLSKFDTTNNFAELTA
                     FRDESYPYEAGYWNKYFATTRTRIDKIEVPATASKGKDVKVTVKLSTVEYPSDVAKLA
                     DSKAKVNLIFITKDKEITYTAKYVSTGTFEVTISGADTKNLTAGSYTLVVEGQLLNEA
                     PAVESSSLVIF"
BASE COUNT         1274 a         1042 c          936 g         1535 t
ORIGIN      
        1 aatgtcattc ccataagcgc tatcatgatt cctggcggta ctacccacca ccacaatcct
       61 gtaagaacag ccccgccctt tagagcatca tgcagtatct gtccccatgt gacgatagtg
      121 gcatctccca gtccgataag acttatagtt gcttcataca caatggcgct tggaacataa
      181 agggccattg aagcaaacga atagggaaca agaatcggaa ccatatgctt gaatatgagt
      241 cttgcatttg aagctcccac agctttggac gcttcaatgt atgtttcttc ttttatctgc
      301 agagccatgc tccttactgt ttttaccgga cctgtccaga agaaagcaca catcattaat
      361 atcattgtcc atatgctggg tttgaaaact gctgacataa ctattaaaac tgggagaagc
      421 ggagtactgg cgaaaagctc aaaaattctc tgcattattg aatcgacaaa tccaccgtag
      481 taggcgctga ttactccgta aacaactcct atgccaactg cgataacagc agtaagtaag
      541 cctataagaa gagcccattt tactcctgca accacacctg accatatatc tctttttgaa
      601 ttatcggttc cgagtatccc tgaaactctt ccggaaacaa ccatgtaagg atcttcgtat
      661 gaagattcct ggtcaagaag tatggtagtc actgtaaatt tgtatatgcc ttttaagggt
      721 tcaattttat tgagcatatc actttttgct tgtgcaaagt atatatcaag aggctttaca
      781 gaggtcttat ccacatactg tagattctct tcgttttcaa acttctgggc aaaatcataa
      841 gccgctgctc cggagtcctt atcgagggta attctgatat catttccgcc gtctgtctga
      901 attggcatct gaaccagatc aaaagtttcc ccgtcgggtc tttccatttt tacaatcata
      961 atcatgttgc ctgtacctct tccgtgaaat atgatatcaa gcggtgcttt gtcgtatttg
     1021 taatcatatt caaaggtact gttgacaaac ttcaaatctc cttcaataac ttcagactga
     1081 tatttggtta ttttttctga ttcgggtgat tttttacttg agaaccagtt ggtccatact
     1141 ggaggtgccc ctgaagggtt gtcgtcccag taagttatgt ctctccagcg ggagcttgcc
     1201 tgtgaaaatg gaactatata tggttcaaag atcattagta agagaaatat tatcagaaat
     1261 acaaaaccga cgattccgaa cttaactttt ctatattcac tccaaaaatc cttaagccaa
     1321 aactttaagt ctttcatttt catgctttac caccaacttt gattctcgga tccaaaaatc
     1381 cgtagattaa gtccagtata accaaaccgg ctatgtataa tgctgttgta attgctaaaa
     1441 gacccattaa aacaggaaca tcattttgct caattgctat ccagtaaagg ttacccatgc
     1501 caggccaact gaagatgcct tcgaaaagga tattaccgct tacagatgac aaaagagata
     1561 acagagccat tgtcactata ggaggtgccg aggttctcat ggtgtgccca aacaaaactt
     1621 tattttcggg tatacctctt gctctggctg acattacaaa atcttcctgc aacgttccga
     1681 gcacaatatt tcttgtaaga taagctgtac cccaaaagtc aataatcaaa agcgtaagca
     1741 tcggtagagc catgtgataa aggatgtcta aaaagtaggc aaacccctga ggtggtggaa
     1801 ccgtatgcag tcctcctgaa ggaaagatat ttacctggta ggcaaagaac ataatcatta
     1861 tcattccaag ccaccatcga ggcattccat atactaccat ggttgctata gaagttgatt
     1921 tatctagaac accgcctgcc cgttgagctt ttttcaagcc aagccatacg cccaatatca
     1981 gctccaaaat cattgcagtg gtaaagagca gaatggttct tgggatagtc tccgatacta
     2041 tggtccagac atccctctcg ccttttgcag actttatgtt tgtggagttt ccgaagttaa
     2101 gggtgatagt atcgatggtt ctccagacta ttctctgaaa tataggttgg tcaaggtgat
     2161 acagatgata cttataggcc cttctttcgg cgatatactt ttgtacttcc tgtacatcca
     2221 tgtttctcat tttcgtagtt tctgctctaa gtgcttcttc aatctgagcc ttagtggttg
     2281 cttcattggt tgcattaaag agtacggaga aaatgaatat caagatgata tacattacta
     2341 ttccgtagaa aattcttttt gcagcatact tccagtacat attttttcac cccgacttcc
     2401 gtttataaaa aataagttgc attattattt tctttttatc aatggtttac aagtcttttt
     2461 attaaaagag tggcaccctg tagctagagt gccacccctc atcataaaat aataataatt
     2521 agaattcgat cagaatataa ctaaagaaga ggattctact gcaggagctt cattaagaag
     2581 ctgtccttca acaactagtg tgtaacttcc ggcggtaagg ttctttgtat ccgctccgga
     2641 gatagttact tcaaaggtgc ctgtggatac gtactttgca gtataggtaa tttccttatc
     2701 ctttgttatg aaaatgagat ttacctttgc ttttgaatcg gctaattttg ctacatcaga
     2761 aggatactct actgttgaaa gtttaactgt tacctttacg tccttgcctt ttgatgctgt
     2821 tgcaggaact tctatcttat caattctggt tcttgtagtt gcgaagtact tgttccagta
     2881 acctgcttcg taaggatatg attcatccct gaaagctgta agttctgcga agttgtttgt
     2941 agtatcaaac ttcgataggt agaaaggacc gttggatatg taagcatgtc cgtaagtatc
     3001 aatgaacttt atagctgctt catagttgct tactgcatcg gctgctgtca tgtaatcttt
     3061 tactgatgcc ggaacatact ttgctgattt catttcttca agtttggctt ttatatctgc
     3121 aacacatttt ggactgagag catcgatctc tgtgaaagct ggatctgaac tgaagctgta
     3181 aactgtaccg gatttgcttc cttcggcaac caatttggcc attgcttctg caatttccca
     3241 ggaaacactt acattgactc cagccggagt aagcttaagc catggagctc ctgttgcggc
     3301 aactctgtcc ggatcaagcg ggaagttgaa atcaaagtaa acggttatag tgttgtctct
     3361 gtttaccaca taacctttaa aggtttccat ctgcgatctt gccaacgatt cgtatgttgg
     3421 ttcatagtac ttgtcgtttg ctccgtcttt attcatccat tctacaagga aagcctgaac
     3481 atacatgatg tctgcaattc ctataggtct tccgctgtgc cattttccaa atttaaagga
     3541 gtaagttgct ttggagaaag atgtaacgcc tgggccaact ttttcccatt tcttggtcat
     3601 ggagttatac tttattgcat cggcaggaac ttgcaatgtt ccaacaagct tatctccctt
     3661 catttctacc tttgtgacaa cgtcttttcc gaaagctctg taagcagtat cttttgcact
     3721 gtttggggct tcaaaatatg atgaatcata caaaggatca gagataacct gtgagtatgt
     3781 atcgctgaac ccatctactc cgataggatc ccatgcgctc atgaaaagtg atccttttgc
     3841 tgaatactgg gtaagtctga gaactttctg accgtttgaa tccggtttta catcagctgt
     3901 tatatgggcc catctgttaa gaccgtcgcc caatccgtaa acgactctgt tattgaatct
     3961 gtctttgttg gccgcaaaat actgatcctg atatgcaagg tatattcttg cagagtcttc
     4021 aagtccgagt tctgtggctc tgaggttgtc ggtccagtac tcttcttctg taaggaagag
     4081 accgttaaat atcttctggc tgagagcatc gatttcgttc tgtttatagt tccagaattc
     4141 cggaactgct ccgcctgcca tgtaaccgta ccatggtgag tacatctgtg ctattgttat
     4201 atcccagaaa gctcttgtgg ctcctgctcc ccaaccttca gtgtaaagac cccattcata
     4261 atctgcggga tctccattgt atgccagctt gacgcacttg gatctgtcgt attcaagtct
     4321 ttcaaccttt ataccggatt tttcaatctg atctgctatg tatcttccgg ccttgagtct
     4381 gccctgagga tcgtcgactc ttataaggaa ctttatggta accggtttgt tgttatacat
     4441 ccaccagccg tctttcttaa caagtttgcc tttgttttcg gcaagtgcgg ccgccttctc
     4501 catagctgca tttatatctg ttatagcttt cttttcattt ccctgagctg tcattccgta
     4561 cttggtagct ataagattgt acctgtatgt gccgggctga cccggggtca taggtgtgaa
     4621 cattgctccg cctgctccac caagtatttc gtcaactaag tactttctgc ttataagaaa
     4681 gttaagtgca aatcttattt ctttcatggc aagaggatta aagaacgttt cgccgctctt
     4741 tacagtccat gtgtagggtg ctgcgttagg aatcgggtta accataa
//
LOCUS       DAOSHR010000154         4734 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_16140_length_4734_cov_4.606540, whole genome shotgun sequence.
ACCESSION   DAOSHR010000154 DAOSHR010000000
VERSION     DAOSHR010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 4734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4734
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16140_length_4734_cov_4.606540"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            35..>4734
                     /locus_tag="PLU28_08350"
     CDS             35..>4734
                     /locus_tag="PLU28_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain repeat protein"
                     /protein_id="HOG35106.1"
                     /translation="MKVLFNLGVGVHTIKIKAVDNAGNPSKEITVPYKRVPDITESTP
                     IIDYIVDPYIEPNADYSITIVATDTKAYITEIKLNDTTIPIIPAQPYVRKQIKQDKQS
                     KVQTLSYTAKAKNLLDTESSVSFKVNISNNNPPLVDVLADNATPNFGDIVNITVRATD
                     DLGLSLVRVNLGSEKIAEYTAKDFIKDKANGSVLNKTIQWTAKNGTFNIEAIAFDIKN
                     ASTSFKRNAFIKVNDTTPPVLNDLYFKVNVGGRGGDIRDILYNVEESTSIKKFSLAKG
                     DIVRFYAQAWDKEQQTDKYSGVSRVVFRVLGGSSDISYDSIYSSNDDWFYSNGQWTVP
                     ESGTYSISLTLTNQQGMVSTYDNYARIDAVGSYEDVYRPKIKNLYVNSTSPSAGVNDI
                     RISDTVTSGAIFADDGKLKIVQIKFYKWQDSNNNGIVDNGEISDIRENPYQLKYVQSN
                     ESTNYGKQDNLYLSWIASTPGKFVASAVATNTLNLTNSATVTFEVPNQYLDIIKPEIT
                     TPPQSRQMGILNDFWSVEVNTNLESDLNVYLFSDDNGNGIIEETSETSMTVRKLKDLV
                     DSKVITKIINGNKTSLNEYRFNVNLSDKDFLDNTKYLFEKTGRYRFVFEARFQDKLSR
                     KSVDVMITDPKPPELTSITCEPTGTSTVAGKSTYKVSDRFYVPIVYSASDPFSKNKTD
                     FDLTFKAVAYGNIDKAQIILQSGEVIEARRVSVADIPDGTGRREYTFNKEIEGSKFQQ
                     PVESKFTIKLIKSDAGIGGSSQNVSTYDYYIVPVEFNRPIINNYEFALSSEINVKKSE
                     VPNKTYIIYAGQGLNLNVYNLDVEDDFAVGGVSIYFKDKSGSILSPVPINKNTLSAFG
                     NRKRIVREDIVNAFGADALSSNYTAPSKADEYELIIRIKDRSYTYLEDANILDKFLLS
                     GDVNYTEEIINVKVVDRTPPAVSMSIQGNTRNIIETQNFYTVVNIDDKFDIVKPETIN
                     LFLIMPNGQRVGPLSPVTADKPYDGTGPYTLGRKIPEEYIGMEGPAKLYVTFDTIIAT
                     NVSYEKNPQDVIIDIDKDTEFIFDKPVRKISMVPDGHIIESNVSVSGRKENYTYDLKD
                     SVVYIGYKKRPATAWTVVKATKYQDKVVKELGDLGTGIYDVYVYAYDYAGNLFKSSTT
                     TFEVETVPPAVSRLIASDQDRNSFVFAFKTNQLFDEDIEKPYISITDNYGLFKVIAEY
                     AVKAGGYGLLEVPSYSANPKVYNIYEPQNPESIKTEERLYIRDFLKGAVGGADASINE
                     GNCIGLRIIATDLNNNNAVFPYKIKDSVYNDIKIFRDNTAPLDFSGNSNFNSTNYMTK
                     TNNKVYLTVDDNVSVEKLEILTFSRDSGGGTLLPLGTKFQQNTIVFGANDWKYEAFTP
                     PDNTEGIYDLVLKATDLAGNYRNSTIKINLDTKVPVLDTASFKVGTVTPISGTYYISD
                     DATPGNISFNVQDRFISSSVGSVNAIIDGVTVRPLVIGSTDSTFKSGASIKISDVLVS
                     GEKEYTMSFEITDGAGNKSVWPSSIRIKYDKSAPVLTAVSGTTTLSESSVYASGADAI
                     NINFTTNETIKYYRV"
BASE COUNT         1581 a          872 c          997 g         1284 t
ORIGIN      
        1 aataaagcga aattactatt ccatacaggg ggaattgaag gtacttttca accttggggt
       61 aggtgttcat accataaaga ttaaggctgt tgataatgcc ggtaatccga gcaaagaaat
      121 aaccgttcca tataaaagag ttcccgatat tacagaatca acacctatta ttgactatat
      181 agtagatccg tacattgaac caaatgcgga ttatagtatc acgatcgttg ccacagatac
      241 taaggcgtat ataacggaga taaaactcaa cgatacaact attcctatta ttcctgccca
      301 gccatatgta agaaaacaga taaagcagga taagcaaagc aaggtacaga ctctttctta
      361 cacagccaaa gctaagaatc ttcttgatac tgaaagcagt gtttccttta aagtaaatat
      421 ctcaaataat aatccgccct tggtggatgt tcttgcggat aatgcgaccc cgaactttgg
      481 ggatattgta aatataactg tcagggccac cgatgatctt ggtctaagtc ttgtcagggt
      541 aaatcttggt tctgaaaaga ttgcggagta tacagcaaag gatttcataa aggataaagc
      601 aaacggaagt gtgctcaata agaccataca gtggacagcc aagaacggaa cattcaacat
      661 tgaagctata gcttttgaca taaaaaacgc ttccacttct tttaagagaa atgcgtttat
      721 aaaggttaac gataccacac ctcctgttct caacgatctt tacttcaagg tgaatgtcgg
      781 tggaagagga ggagatatca gagatattct ttataatgtc gaagaaagca cttctataaa
      841 aaagttcagt ctggcaaagg gagatatagt aaggttctat gcacaggcat gggataaaga
      901 acagcagact gataagtact cgggagtatc aagggttgtt ttcagagttt tgggcggttc
      961 ttccgatatt tcctatgata gtatatactc ttcaaacgat gactggtttt actccaacgg
     1021 tcaatggact gttccggagt ccggaactta cagtatttct cttacgttga ccaatcagca
     1081 gggcatggta agtacgtacg ataattatgc acgtatagat gcagtgggaa gttacgaaga
     1141 tgtatacagg ccaaaaataa aaaatcttta tgtaaacagc accagcccga gcgccggtgt
     1201 gaacgatata cgtatatcgg atactgttac ttccggagcc atatttgccg atgatggtaa
     1261 gcttaagata gtgcagataa aattttataa atggcaggac tccaataata acggaattgt
     1321 tgataacggc gagatctcag atataaggga aaatccatat cagctcaagt atgtacagtc
     1381 taatgaaagt accaattatg gaaagcagga taatctatat ctttcatgga ttgcttccac
     1441 gcccgggaag tttgttgctt ccgccgttgc gactaatact ctcaatttga ccaattcagc
     1501 gactgttacg tttgaagttc ccaatcagta tcttgacata atcaagcctg aaataacaac
     1561 gcctccgcaa tccagacaga tgggaatact taatgatttc tggtctgtgg aagttaacac
     1621 caatcttgaa tcagatctta atgtctatct tttctcagac gataatggaa acggcataat
     1681 tgaagaaact tcggaaactt ctatgacagt gagaaagcta aaggatctgg tggactctaa
     1741 ggttattacc aagattatca acggcaacaa gacgtctctt aatgagtaca ggttcaatgt
     1801 gaacctcagc gataaagatt ttcttgataa taccaagtat ctttttgaaa agacagggag
     1861 ataccgcttt gtctttgagg caagatttca ggataaactt tcgcgaaaga gcgtagacgt
     1921 catgataacc gatccgaagc ctcctgaact caccagtatt acatgtgaac ccacaggaac
     1981 aagtaccgtt gccggaaaaa gtacctataa ggtttctgac agattctatg tacctatagt
     2041 gtactcggca tctgatcctt tttcaaaaaa caaaacagat tttgatctta cttttaaagc
     2101 cgttgcgtac ggcaatatag ataaagcgca gatcatactt cagtcaggtg aagtgattga
     2161 ggcacggaga gtttctgtgg cggatattcc tgacggaaca ggaagaagag agtacacctt
     2221 taataaggag atagaaggga gcaagtttca gcagcctgta gaaagcaagt tcaccataaa
     2281 acttattaaa agtgatgccg gaataggcgg ttcttctcag aacgtttcga catacgacta
     2341 ctatattgtg ccggttgagt ttaacagacc tattataaac aattatgagt ttgctctttc
     2401 ttctgaaata aatgtcaaaa aatctgaggt tcccaataaa acatatataa tatatgctgg
     2461 gcagggactt aatttaaatg tatataacct tgatgtggag gatgattttg ccgtaggagg
     2521 agtatcaata tactttaagg ataagagcgg tagcatcctt tcacctgttc caataaacaa
     2581 aaataccctt tcagcattcg gaaacaggaa aagaatagtt cgtgaagaca tagtgaatgc
     2641 ctttggtgcc gatgccttgt cctccaacta tacagctccc agtaaagccg atgaatacga
     2701 acttataata agaataaaag acagatcgta tacttatctt gaggatgcca atatactgga
     2761 taagttcctg ttgtcgggag atgtaaacta tactgaggaa attataaacg tcaaggtggt
     2821 tgacaggact cccccagcag taagcatgag tattcagggt aacacaagga acataataga
     2881 aacccagaac ttctacacgg tagtcaacat agacgataag tttgatatcg tcaaacctga
     2941 gactataaac ctttttctta taatgccaaa cgggcagagg gtcggacccc tttctcctgt
     3001 taccgccgat aagccttatg atggaacagg tccatatacc ttgggtcgaa aaataccgga
     3061 agagtatata ggtatggaag gtcctgcaaa gctatacgtt acctttgata ccataatagc
     3121 cacaaacgtt tcttatgaaa aaaatccaca ggatgtgata atagatatag ataaagacac
     3181 agagttcata tttgataagc ctgtgaggaa gataagcatg gttcctgacg gacatatcat
     3241 tgagagtaac gtaagtgtat caggcagaaa agaaaattat acttatgatc ttaaagattc
     3301 agtagtttat ataggctaca agaaaagacc ggctactgca tggacagttg taaaggctac
     3361 aaagtatcag gataaagtgg tgaaagagct cggagatctg ggaacaggga tatacgatgt
     3421 atatgtctat gcatatgatt atgccggcaa tcttttcaag agcagtacca caacatttga
     3481 agttgaaact gttcctcctg cagtttcaag acttatagcc tctgatcagg atagaaacag
     3541 ctttgtgttt gcatttaaaa ccaatcagtt gtttgacgag gatattgaaa aaccgtacat
     3601 ttctattaca gataattacg gtcttttcaa agtgattgca gaatatgcag ttaaggcagg
     3661 tggctatgga cttttagaag taccgtcata ctctgccaat cctaaagtgt ataatattta
     3721 tgaaccacaa aatcccgaaa gcataaagac agaggaacgt ctttacataa gagacttttt
     3781 aaagggtgcg gttggaggag ccgatgcgtc gataaatgag ggaaactgta taggtctgag
     3841 gataattgca acggatctga acaacaataa tgcagtattt ccatacaaga taaaagacag
     3901 cgtttacaac gatattaaaa tattcaggga taacacagct ccattggact tttcgggaaa
     3961 tagcaacttt aactcaacca actatatgac caagactaac aacaaggtat atcttacggt
     4021 tgatgataat gtaagtgtcg aaaagcttga gattcttacc ttcagccgtg acagcggagg
     4081 cggaacactg ctacccttag gaaccaagtt ccagcagaac accatcgtat ttggggcaaa
     4141 tgactggaag tacgaagcct ttaccccccc ggacaatacg gaaggaatat atgatctggt
     4201 tcttaaggct acggacttag caggaaatta cagaaattca accataaaaa taaatctgga
     4261 tacaaaggtt ccggttctcg atactgcttc gttcaaagta ggtactgtaa ctccgatttc
     4321 tggtacttac tatatttctg acgatgctac cccaggaaat ataagtttca atgttcagga
     4381 cagattcatt tcttcttctg ttggcagcgt taatgccata attgatggtg tcacagtcag
     4441 accacttgta ataggctcta cggattcgac atttaagtct ggggcatcaa taaagatttc
     4501 tgatgtactg gtatcaggtg aaaaagagta tacgatgagt tttgaaatta ccgatggtgc
     4561 aggcaataaa agtgtttggc cctccagtat aaggataaag tacgataagt cagcgccagt
     4621 tcttacagct gtttccggca ctacaacact aagtgaatca tccgtgtatg catcaggagc
     4681 tgatgctata aatattaact ttactacaaa tgaaaccata aagtactacc gtgt
//
LOCUS       DAOSHR010000155         4714 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_16232_length_4714_cov_5.005151, whole genome shotgun sequence.
ACCESSION   DAOSHR010000155 DAOSHR010000000
VERSION     DAOSHR010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 4714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4714
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16232_length_4714_cov_5.005151"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..986)
                     /locus_tag="PLU28_08355"
     CDS             complement(<1..986)
                     /locus_tag="PLU28_08355"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG35107.1"
                     /translation="MKPFFSQFMIDLIVARRNMFFIIVLSIALLYSMIVNFVIPSKTE
                     SYSINFYILDASVNAKYKQALMSSDYKTFTVSSLDSIKESMSKDKSALGLVLKDDGIE
                     IVFQGYESLQQRNIIKSIFSVLYDTLNNQNSIAAYKTTVLKKSAKKLPLNMKMIPVLL
                     ATDIIMLGFMFVAAMIFQEKKEGGLYAYRVSPCGTLNYILSKVIVNVMISVLFGVVFL
                     FFTLGFKTNYFYFLSMIFLGAFLISLLGIFLAQFYSSLNEFIVVALIFNAVMALPVVS
                     YFNPLIDIQIFKIIPSYSLIYASDEIISSVKNSYTLNTLLNLFIQISIFFPAT"
     gene            complement(986..1840)
                     /locus_tag="PLU28_08360"
     CDS             complement(986..1840)
                     /locus_tag="PLU28_08360"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG35108.1"
                     /translation="MISVQNLSHKYPGNSDYSVRGISFDIGKGEIFGFLGPSGAGKST
                     VQKILTGLLKKQEGHILIEGTEIDLLNKDFFEKIGVSFELPNLYEKLTGYENLKYYAG
                     LFSRKTFDPLQVLSWVGLENDAHKKASAYSKGMKQRLVFARAVINDPELLFLDEPISG
                     LDPATALSVKRVITDFKDRGKTIFLTTHNMFLADEICDNIAFLNEGQILAMDTPKKLK
                     LKYGSYSVNVEYRKDKKILTELFFLDKDEEKKRLKEVIDGYEIITMHSREATLEEIFI
                     KLAGRGLN"
     gene            complement(2081..2743)
                     /locus_tag="PLU28_08365"
     CDS             complement(2081..2743)
                     /locus_tag="PLU28_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35109.1"
                     /translation="MKKIITVVMTLVLAIGIFAAYPTYNGITDPAYEHVINGNNLELN
                     NKTVNVKIMQWISGNFINRATLNSTRGLKEGTNYISSDIVLTKPGLVKDQIFGAIAVI
                     SNGLVDISVSKNNEYAPSNKINISENVSGLKLVEFNRLNKFTQVAYTFTKDIADAIPA
                     TGYALSNSSVTGLKGNSKLDGRVVGPEFLIAGDIDVPFDMGPADNYIVAFDIVVSPKG
                     KF"
     gene            complement(2917..>4714)
                     /locus_tag="PLU28_08370"
     CDS             complement(2917..>4714)
                     /locus_tag="PLU28_08370"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOG35110.1"
                     /translation="HPKDNQEEIKKLMKSVFLGDRTSRLRPLITSENNIIYVETRIWA
                     AQWNGKESIFVISKDVTKENILLNKLKNVFENNPASMSIRDFPTGIFTDANEVFLNKY
                     GYKKTDILGKTLEEIGIFIDEATNKEIEQELLKNGKVSLREVRITTSSGRIIQNLFTG
                     AILDDLNKKSFLTVTIDVTGQKQAEDNLKEAEKRFSLALEGAEMGLWDWDMINNKVYF
                     SKLWKSMLGYDENEIENSFEGWKKLWHEDDVQKIEKAIKDYLNGKTEKYEIIHRLKTK
                     NNSWKWILTRGSLLRQKDGTPYRWVGTNVDITNEKERSDELERFFSVNLDLLCIADID
                     GNFLKVNSEWINVLGYSREYLEKSKFLDFVHPDDIPNTLEAMSKLSQQHEVVDFVNRY
                     RAKNDEYRYIEWRSHPYGKIIYAAARDITDKIETEKKYREIAIRDPLTEVYNRRHLFE
                     RLSSMISEHTRTKKDFSVAILDIDFFKKINDTFGHQAGDYILKEFTKTITHNLRPYDV
                     LGRYGGEEFMIILDWVSKETALIIVERILTDVRQKKYVYGDATICFTFSAGISDCKDA
                     EEDFTVEKLVEKADSRLYSAKHSGRNTIIITD"
BASE COUNT         1444 a          879 c          719 g         1672 t
ORIGIN      
        1 gttgccggaa aaaatatact tatctgaatg aaaagattca agagggtatt cagagtgtaa
       61 ctgtttttga cagagctaat tatttcatca gatgcatata ttaacgaata agacggtatt
      121 attttaaata tttgtatatc gatgagcgga ttgaaataac ttactaccgg taaagccatg
      181 actgcattga aaataagtgc aacaactata aattcattaa gtgagctata gaactgggca
      241 agaaaaattc ctaaaagaga tataagaaat gctcctagga atatcatact tagaaaatag
      301 aagtaatttg ttttgaagcc taaagtgaaa aaaagaaaaa caactccaaa caaaacagat
      361 atcataacgt ttactataac ttttgataaa atataattaa gagttccaca aggacttact
      421 ctataagcat ataatcctcc ttcttttttt tcctgaaaga tcatggctgc aacaaacata
      481 aacccaagca ttataatatc tgttgccagt aaaacaggaa tcattttcat atttaaaggc
      541 agtttcttag ctgatttttt gagtacggtt gttttgtagg ctgcaataga attctgattg
      601 ttgagagtgt catataaaac agaaaagata ctttttatta tatttcgctg ctgtaatgat
      661 tcatatccct gaaatactat ttctattcca tcatccttaa gaacaagtcc aagagccgat
      721 ttgtcttttg acatgctttc tttaatagaa tcaaggcttg aaactgtaaa tgttttataa
      781 tctgaagaca taagagcctg tttatatttt gcattgacgg aagcatctaa gatataaaaa
      841 ttaatggaat atgattctgt tttggaagga attacaaagt taactatcat ggaatagagc
      901 aaagctatgg aaagaacaat tatgaaaaac atatttcttc ttgctactat aagatcaatc
      961 ataaactggc taaaaaacgg tttcatcagt ttagccccct tcctgccagc tttatgaata
     1021 tctcttcaag cgttgcttct ctgctatgca tagtaattat ttcatatccg tctattactt
     1081 ccttcaatct ctttttttct tcatccttat ccaaaaagaa aagttcagtt agaatttttt
     1141 tatctttacg atattcgaca ttaactgagt agcttccgta cttcaacttc agttttttag
     1201 gtgtatccat tgccagaatc tgtccttcat taaggaacgc tatattatcg catatttcat
     1261 cggccagaaa catgttgtga gtggtgagaa aaatggtttt ccccctatcc ttaaaatctg
     1321 ttatgactct tttaactgat aatgcagttg caggatcaag tccagaaatc ggctcatcca
     1381 ggaaaagcag ttcaggatca tttattactg ctcttgcaaa aaccagcctt tgcttcatac
     1441 ctttagaata agctgatgct tttttatggg catcgttttc taggcctacc catgaaagta
     1501 cctgtagtgg atcaaaagtt ttccttgaaa acagtccagc gtaatactta aggttttcat
     1561 atccggtaag tttttcatat aaatttggca actcaaaaga aacacctatc ttttcaaaaa
     1621 aatctttatt tagtaaatct atttcagtcc cttctatcaa aatatgacct tcctgttttt
     1681 tcaacagtcc agtaagtatt ttttgaactg tgctcttacc tgcgccactt ggtccaagaa
     1741 agccaaatat ctcacctttg ccaatgtcaa agcttattcc tcgtactgaa taatctgagt
     1801 tcccgggata cttatgtgat aaattctgca cacttatcat atattcctcc ttcataccat
     1861 acggtctaga cctactagtc tagactggta ggtctattat atcatattgt tatttagttg
     1921 tcaaataaaa aaatccgtca gatttttgac ggatttacta atatatattt ttcaaaaatt
     1981 tatagccgta aaaaagctta gactcaagtg ctcacagtat ataaggcata gcactcataa
     2041 aaaaatctcc ctgaaaaata ctcagggaga gcaaatttgt ttagaatttt ccttttggcg
     2101 atactactat atcaaacgct acaatataat tatctgcagg tcccatatca aaaggaacat
     2161 caatatctcc agctataaga aattccgggc ctaccactct tccatcaagc tttgaattac
     2221 cctttagccc tgttacagaa gaatttgaca atgcatagcc tgttgcaggt attgcgtcag
     2281 caatatcttt tgtaaaggta taggcaactt gtgtgaattt gttcaatctg ttgaattcaa
     2341 ctaatttcaa accgctcacg ttttcactta tattgatttt gttcgaagga gcgtattcat
     2401 tatttttact tacactaata tcaacaagcc catttgagat aactgctata gctccaaata
     2461 tctggtcttt tacaagtcca ggtttggtta gtactatatc agaagatata taattggtac
     2521 cttctttaag tcctctggta ctgttaagtg ttgccctgtt tatgaaatta ccagatatcc
     2581 actgcattat ttttacatta acagttttat tgtttagttc cagattgttg ccattaatta
     2641 catgttcata agcgggatct gttattccgt tataggtggg atatgcagca aatattccaa
     2701 tagccaaaac aagtgtcata acaactgtga tgatcttttt cattagttat acctccatat
     2761 attttttcat cttaattctg actatagaat acgctatata tgttaacggt caagctctaa
     2821 aattacgttt ttataaaata aaaggaaacg ccaagaaaca tatagatgca ttttttactt
     2881 tctttttact tatgatcagc atatatgctt tttagatcaa tctgttatga ttattgtatt
     2941 tcttccggag tgcttggcgg agtaaagcct gctatctgct ttttcaacaa gcttttcaac
     3001 tgtaaaatct tcttctgcat ctttgcagtc agatattccc gcactgaagg taaaacagat
     3061 tgtagcatct ccatagacat acttcttttg ccgcacatcg gttaatattc tttcaacgat
     3121 aattaaggct gtttctttac ttacccagtc aaggattatc ataaattctt ctcctccata
     3181 tcttcccaaa acatcgtaag gacgaaggtt atgagttatt gtttttgtaa actcttttaa
     3241 tatataatct cctgcctgat gaccaaacgt atcgtttatt tttttaaaaa aatcaatatc
     3301 aaggatggca acagaaaagt ccttttttgt tcttgtgtgt tctgaaatca tcgaagataa
     3361 tctttcaaaa agatgtctgc ggttataaac ctcagtaaga ggatctctta tagcaatttc
     3421 tctgtacttt ttttccgttt cgatcttgtc ggttatgtcc cttgcagcag cataaatgat
     3481 cttgccataa ggatgggatc tccattctat atatctgtac tcatcatttt tggcacggta
     3541 gcggttaaca aaatcaacaa cctcatgctg ttgtgaaagc ttagacatag cttccagtgt
     3601 gtttggtata tcatcaggat gaacaaaatc aagaaacttg cttttttcaa gatattctcg
     3661 cgagtatccc agaacgttta tccattcact gtttactttt agaaaattcc catctatatc
     3721 ggctatacac aaaaggtcaa ggttaaccga aaagaatctt tccagttcat ccgaacgctc
     3781 tttttcattt gttatatcaa catttgttcc cacccatcgg taaggagtac cgtctttttg
     3841 tcttaacaag ctgccgcggg tgagaatcca tttccaggag ttgtttttcg ttttcagtct
     3901 gtggataatt tcgtattttt ctgtctttcc attgagataa tctttaattg ccttttcaat
     3961 tttctggaca tcatcttcat gccaaagttt tttccatcct tcaaaagaat tttcgatctc
     4021 attttcgtca taaccgagca tacttttcca taattttgaa aaatatactt tattattaat
     4081 catatcccaa tcccaaagtc ccatctcagc gccttcaagg gcaagagaaa atcttttttc
     4141 tgcttcttta agattatctt cagcttgctt ttgacctgtt acatcaatag taaccgtaag
     4201 gaagcttttt ttgtttaaat catccaaaat agctccggta aaaagattct gtatgattct
     4261 gccagaactg gtagttattc gcacctctct tagagatact ttcccatttt ttagaagttc
     4321 ttgttcaatc tctttatttg tggcttcatc tatgaaaatt ccaatctctt caagagtttt
     4381 cccaagtatg tcggtttttt tgtatccata tttattcaaa aaaacttcgt ttgcatctgt
     4441 gaaaatacct gtggggaaat cacgtattga catggaagca ggattgtttt caaaaacatt
     4501 tttcagcttg ttaagcagaa tattttcctt tgtaacatcc ttagaaataa caaaaatgct
     4561 ttctttccca ttccattgag ctgcccatat tcttgtctcc acataaataa tattattttc
     4621 tgaagtaata agaggacgca gacgggaggt tctgtcacct agaaaaacag atttcattaa
     4681 tttcttgatt tcttcttggt tatctttcgg atga
//
LOCUS       DAOSHR010000156         4685 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_16365_length_4685_cov_5.292009, whole genome shotgun sequence.
ACCESSION   DAOSHR010000156 DAOSHR010000000
VERSION     DAOSHR010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 4685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4685
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16365_length_4685_cov_5.292009"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..615)
                     /locus_tag="PLU28_08375"
     CDS             complement(64..615)
                     /locus_tag="PLU28_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35111.1"
                     /translation="MIDLIKVMSSERFFEVTEYLKLNPNVNASSISSNLNIHIITVQR
                     YLEVLEKYGFVSSREKRGIGRPSKTYTFLGGKFTLDIDELLKEYSLRSQKIREKENST
                     ISYNYDIDNERIKQVIIGGRKGTKVELEDKSGRFLWMLPPPDSDGESIEKLASQSGMA
                     VLDAVKFVRDMIEIGVMEVCSDE"
     gene            complement(721..795)
                     /locus_tag="PLU28_08380"
     tRNA            complement(721..795)
                     /locus_tag="PLU28_08380"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(761..763),aa:Val,seq:cac)
     gene            complement(841..2445)
                     /locus_tag="PLU28_08385"
     CDS             complement(841..2445)
                     /locus_tag="PLU28_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35112.1"
                     /translation="MKKLLVVLAVLAMIVSGFAGASIATSGPNFTASLVSGGSVSFGL
                     YFDENGLDLGNAISMKYSASFTIGLNVSASDSISYPATLTLGLSATEPEWQESLNKKP
                     LSFNNGRYEDNLLNLYFKNGAYYGSWSKYTLFNEDANGDGKMDYTPVGRQYITTNFKP
                     ITLDSIYITMSKETTYSPHATTNNATQLLFADFFAFKKTFDTDMAKLNAYAAFWAANK
                     GNPAGNKARPATIEASSLTLDGKWLGGSAEAELTAKGILEGLTANFAAGAESKVKAAD
                     FKDFAYYAMVNYSKGYKVLETPSVTVTPHAKFQYNTLMNVLPFVKDTIAASWVNAGAD
                     LALDMGAVGTFGLKDTVTFAFGKNVYGTDKNLKYYYGLTYTNKTLAFANVYGEFAKDE
                     GTELATPFGLNVKLNGSYTMAPVTVSYLAEVAKVNAGGASKLVPLSDISKVTEKYFAY
                     QATLKVDIIPSLVNLEAKVINYTTKDGEYTAIHKIAEPVYYGTLAYTPNKFWKVAAHV
                     GTDDGSSWGKFGNIHWNLSAVCSVSF"
     gene            complement(2630..4030)
                     /locus_tag="PLU28_08390"
     CDS             complement(2630..4030)
                     /locus_tag="PLU28_08390"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOG35113.1"
                     /translation="MKKLLTLMLILSLVALAFAGPFKDVREDNRYYDYIVNLVDKGVI
                     KANPGTEFKGTSPLTKEEAVVWMTKSVSYVENSPLIAKSVDVQDLKETMEKDVNSKLT
                     NYDTKIANYDSKFADLDSKINALAAKIAVAEKSLSGYNDRFDNIEKTIKYHSDLMGIL
                     MDRMAAVDAQSKENTANFAELVDKVMYIDEKAVSAQANIMVLKNKMEELSKTFDEKHQ
                     ESVAALSEEIMVSKYFVLGKVDEVNARFDDVYLKLNELNTKAAEWKKYEATLKTVDTT
                     YTEVKKSAFIGKNTADSVAEDMLSLVDDIDDLNAKIEELNTKAAEWKKYEATLKTVDT
                     TYAEVKKSAFMGKNTADSVATDMLDLMDTMDENNEITNIRFEGLEEGVTINSYDIVEI
                     KSKLGILNDLDTRLTALEAKASTDVATLGERVTALETKLPEVEKKIPDTTFLWVLSIL
                     GVGLGIVGVLTGIVIK"
     gene            complement(4113..>4685)
                     /gene="tyrS"
                     /locus_tag="PLU28_08395"
     CDS             complement(4113..>4685)
                     /gene="tyrS"
                     /locus_tag="PLU28_08395"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOG35114.1"
                     /translation="PQVVLTMPLIEGTDGNLKMSKSYDNYIAFNDTSRDMFGKIMSIP
                     DTLIIKYMRLLTDLGKTEIDLYESQMKDGLVNPRDIKMKLGQEVVAVFYGREEGVKAQ
                     EEFIKIFRKKDLPDEMETLKATSPISVLDLVMLSGETPSKSEAKRLIAQGAVKLNEEK
                     LDDFSRMLEVKGSEVLKIGKRKFFRIDLAF"
BASE COUNT         1268 a          971 c          846 g         1600 t
ORIGIN      
        1 cgtaatagta tctggagtat gaacataaag gatacaaaaa ataaaacaaa aaaaatatta
       61 tcttcattca tcgctgcata cctccattac tccaatctct atcatatcac gtacaaactt
      121 aactgcgtca agcacagcca tgccgctttg actggctagt ttttctatac tttcaccgtc
      181 cgaatcaggc ggaggaagca tccacaagaa ccttcctgat ttatcttcaa gctcaacctt
      241 agttcccttg cgtccaccaa ttatcacctg ttttatacgt tcattgtcta tatcataatt
      301 atagcttata gtggaatttt ctttttctct tattttttgg cttctcagac tgtactcttt
      361 taaaagctca tcaatatcaa gagtaaactt cccccctaaa aaagtatagg ttttggatgg
      421 ccgtcctatt cctctttttt cgcgtgaaga aacaaatcca tacttttcaa ggacttcaag
      481 atatctttga accgtaatta tgtgtatgtt caggttgctt gaaatacttg aagcatttac
      541 attagggttt agcttcaaat actctgtaac ttcaaaaaaa cgttctgatg acataacttt
      601 tataaggtct atcatattca gcctgcctat tatttcactt cgagtgaaat aatatcatat
      661 ttatagttat atgtcaagga cgcacgaaaa aaaagaataa cctttaaggt tattcttttt
      721 tggtgggcac agcagggatc gaacctgcga cctcttccgt gtgaaggaag cgctcccccg
      781 ctgagctatg tgccctaaaa aggacggtct tgcgaccgtc ctgattatac ttttttcaga
      841 ttagaacgat actgaacata ctgctgagag attccagtga atgtttccaa acttacccca
      901 gcttgaaccg tcatctgttc ctacgtgtgc agcaactttc cagaatttat taggagtgta
      961 tgcaagtgtt ccgtagtaaa caggttcagc aatcttatga atagctgtgt attcgccgtc
     1021 tttggttgtg tagttgatta ctttagcttc aagatttact aatgaaggaa ttatgtctac
     1081 cttcaatgtt gcctgatatg caaaatactt ctcggttacc tttgaaatat cagaaagagg
     1141 aaccaatttg cttgctccgc ctgcatttac ctttgcaact tcagcaagat agcttactgt
     1201 tacaggagcc atagtatatg aaccgttcaa ctttacattt aatccaaacg gagtagcaag
     1261 ttctgttcct tcgtctttag caaattcgcc gtaaacattt gcaaaagcga gagtcttgtt
     1321 ggtatatgtt aaaccgtagt agtactttaa gtttttatct gttccgtaaa cattctttcc
     1381 aaaggcaaaa gtaactgtgt ctttaagacc gaatgttcct acagcaccca tatcaagggc
     1441 aagatcggca ccggcattta cccatgatgc agcgattgta tccttaacga aaggaagaac
     1501 gttcataaga gtgttgtact ggaactttgc gtgaggagta actgttactg aaggtgtttc
     1561 aagaaccttg taaccctttg agtaattaac cattgcatag taagcaaagt ctttgaaatc
     1621 tgctgctttt actttgcttt ctgcaccggc tgcaaagtta gctgttaagc cttcaagtat
     1681 tccttttgca gtgagttctg cttctgctga tccgccaagc catttaccgt ctaaggttaa
     1741 agaacttgct tctatagtag caggtcttgc cttatttcct gcaggattac ctttgtttgc
     1801 tgcccagaat gctgcataag cattcagctt tgccatatct gtatcaaaag tctttttgaa
     1861 tgcaaagaag tcagcaaaaa gaagctgagt tgcattgttt gttgtagcat gtggtgaata
     1921 agttgtttcc tttgacatgg ttatgtaaat agaatcaaga gtgatgggct taaagttggt
     1981 tgttatatac tgtcttccaa caggcgtata gtccattttg ccatctccat tagcgtcttc
     2041 gttgaaaaga gtatactttg accagctacc ataatatgca ccgttcttga agtagaggtt
     2101 taacaaattg tcttcgtatc ttccattgtt gaaagacaga ggttttttat tgagagactc
     2161 ctgccattca ggttctgttg ctgagagacc aagtgtaaga gttgcagggt aagatattga
     2221 atcagaagcg cttacattca aacctattgt gaaagaagca ctatacttca ttgatatagc
     2281 atttccaaga tcgagaccgt tttcatcaaa gtaaagtccg aatgaaactg aaccgcctga
     2341 aaccaagctt gctgtaaagt ttggaccgct tgtggctatc gaagcaccgg caaaaccaga
     2401 aactatcata gctaataccg ctaatactac taaaagcttc ttcatgaaac caaaccccct
     2461 ttaaaaaatt tttttaggtt aaaaacttca aactatttga ttataccaaa gtcattagct
     2521 ctgtgtcaat agttttaatc ttgttacata agacggttta ccggtattag aggattatac
     2581 gtttacatac agcgataaac cgtctctaat taaggttttt cagtaagtat tactttatta
     2641 cgataccagt gaggactcct actattccaa ggcctactcc aagaatcgac agcacccaga
     2701 ggaaggttgt gtcaggaatc tttttttcta cttcaggaag cttggtttca agagcagtta
     2761 ctctttcacc gagtgtggca acatctgttg atgctttggc ttccagagct gtgagtcttg
     2821 tatcaagatc attaagtatg ccgagtttgg actttatttc tactatatcg taagagttta
     2881 tggtgactcc ttcttcaagg ccttcaaatc ttatgttggt aatttcgtta ttctcatcca
     2941 tggtatccat aagatcgagc atgtctgtag ctactgagtc tgctgtattc ttgcccataa
     3001 atgcactctt tttaacttct gcataggtgg tgtcaactgt cttaagagta gcttcgtact
     3061 ttttccattc tgctgctttg gtgttgagct cttcgatctt ggcattcaga tcatcaatat
     3121 cgtcaacaag cgaaagcata tcttctgcaa cggagtcagc ggtgttcttg cctatgaatg
     3181 cgctcttctt aacttctgta tatgtagtgt caactgtttt aagagtagct tcatactttt
     3241 tccattctgc tgctttggta ttgagttcat tgagcttgag gtatacgtcg tcaaatcttg
     3301 catttacttc gtcaacctta ccaagaacaa agtactttga taccataatt tcttcggaaa
     3361 gagcagcaac tgattcctga tgtttttcgt caaaagtttt ggaaagttct tccattttgt
     3421 ttttcagaac cattatattg gcctgtgcgc ttactgcctt ttcatcgata tacataactt
     3481 tatcaacaag ttcggcaaag tttgcggtgt tttctttcga ctgagcatca actgctgcca
     3541 ttctgtccat aagtattccc attaaatcgg agtgatactt tatggtcttt tctatattgt
     3601 caaacctatc gttgtatccg ctaagacttt tctctgcaac agcaatcttt gctgcaagag
     3661 cgtttatttt tgagtccagg tctgcaaact ttgaatcata gttggctatc ttcgtatcgt
     3721 agtttgttaa cttggagttc acatcttttt ccatggtttc tttaagatcc tgaacatcaa
     3781 ctgattttgc tataagaggt gagttttcaa cataagaaac agatttggtc atccatacaa
     3841 cagcctcttc ctttgtaaga ggagatgttc ctttgaactc ggttccggga ttagctttta
     3901 tcactccttt gtcaacaagg ttaactatat aatcatagta tctgttgtct tcccttacgt
     3961 ctttgaaggg accagcaaaa gccaatgcta ccaaacttaa gattaacatg agcgtcagta
     4021 actttttcat ccttttttac cccctttatt aagttatgta tcttttttat ttcattatat
     4081 acaaatttat tatactacaa cagcgcgctt tttcaaaatg ctagatcgat tctaaaaaac
     4141 tttcttttgc cgatttttaa tacctctgat cccttcactt ccagcattct ggagaaatca
     4201 tcaagctttt cttcgttcaa ctttactgca ccttgtgcga taagtctttt ggcctcactt
     4261 ttgctgggag tttctccgct gagcatcacc agatccagaa cacttatagg agaggtggct
     4321 ttcaaagttt ccatttcatc gggaaggtct tttttacgga atattttgat gaactcttcc
     4381 tgtgccttta ctccttcctc ccggccgtaa aacacagcca caacttcctg tccgagcttc
     4441 atcttaatat ccctgggatt tacaagacca tctttcatct gtgactcgta aagatctatc
     4501 tcagtttttc caaggtcggt cagcagtctc atatacttta ttataagagt gtcgggaatg
     4561 gacattatct ttccgaacat atctctggag gtgtcattga aagctatgta attatcgtag
     4621 cttttgctca tctttagatt gccgtctgtt ccttctatca aaggcatggt aagaaccacc
     4681 tgcgg
//
LOCUS       DAOSHR010000157         4666 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_16442_length_4666_cov_4.122316, whole genome shotgun sequence.
ACCESSION   DAOSHR010000157 DAOSHR010000000
VERSION     DAOSHR010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 4666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4666
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16442_length_4666_cov_4.122316"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="PLU28_08400"
     CDS             complement(<1..337)
                     /locus_tag="PLU28_08400"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HOG35115.1"
                     /translation="MRIFSPVTLDQALLLRSELKCTVLSGGTDLMLKKQFLLSDEHDL
                     LMLQKIEELHEISVKDGITHIGACATQSDILESSLLWGVFRDVVSCMSCPSIRNLATI
                     GGNICNASPA"
     gene            complement(340..822)
                     /locus_tag="PLU28_08405"
     CDS             complement(340..822)
                     /locus_tag="PLU28_08405"
                     /inference="COORDINATES: protein motif:HMM:NF013923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOG35116.1"
                     /translation="MKINFFLNHIFCTVDTDPLRSLADVLRNDFNQTDVKQGCKEGEC
                     GACAVLIEGVTVHSCIYPVVMAQDKKILTLSGILDTAEYQAIKKGFSDAGSVQCGFCT
                     PGIVIVCYDMFKRNIKPSRDMLMEHLSGNLCRCTGYNSIFKGVELAFEYYKKNMVSGG
                     "
     gene            complement(812..2953)
                     /locus_tag="PLU28_08410"
     CDS             complement(812..2953)
                     /locus_tag="PLU28_08410"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HOG35117.1"
                     /translation="MSASISDPVLRNDFEEKISAAAMYVDDLTFKDMLYAKTVRSQRT
                     RAKIKNITVPYLDDGYFTVDYKDIPASNIVKEILDDQPFFANGEVNFIGEPIMLIVGT
                     DPHKVAELAEKVVIDYEDLTPVLSYEDSLRKDIIPIYGLDNCFAEYELCRGNAKKAFA
                     TAEKVVERTYETGYQEHVYMEPQGMIAIYENRTITVYGSMQCPYYIKHALEKAFGWGG
                     ERIRIVQTATGGAFGGKEEFPSIIAGHAAFAAFKSGKPVKLVYEREEDIISTTKRHPS
                     VITVKTALDLNHKITAVDIDIKLNAGAYAGLSSVVLQRAMFCSTGVYNFENFIIKGKA
                     YATNTVPTGAFRGFGGPQAIFAIETHMSALAFMLHQDVVAFKSIYIVKTGDKTTTGGT
                     FRGPLKLTEIINRVKEMADYKKLCSEKNSEDHIKIGVGFSVFLHGCGFTGSGEQQVIK
                     ARALLKKNHDGTVGIFISNVEMGQGLGTVMRKIVASSLGIDISKVIYAPVDTDKVPDS
                     GPTVASRSTMIVGGLLVKACEELKQRWDEEGIVEVQADYQQPSWLEWDNELLKGDAYP
                     SYSWGADIALVSVDTLTYELSIEKIWTVMDIGIPIDTNTLHGQIEGGILQGVGYSSCE
                     VMSMKDGKILQKNLTDYIIPSSKDSVEISLDYIINPYENGPFGAKGAGEIPFLGVGPA
                     VAGAVLDAVKKPVCKLPLTAEYLMEVIENED"
     gene            3222..4622
                     /locus_tag="PLU28_08415"
     CDS             3222..4622
                     /locus_tag="PLU28_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOG35118.1"
                     /translation="MEKIPVIGPTFEEMLHPWKIEETIRRTAWRKRTEDPLHPINLYN
                     ITWRDINNHFYFFEVPHELTGIKANIVVMYAKDFPSESHKVGATYAILAEKTVTGEVD
                     PAVHTLVWPSTGNYGIGGAWVGSRMNYDSIVLLPEKMSRERFEIIRSYGAKAVATPGC
                     ESNVKEIYDKSKELYNSDREHVRVLNQFEEFGNYRFHWYVTGNSMIELVRENKIGNQK
                     IAAVVLGVGSAGTLASADRVKQEFADCKCVAMEPVQCPTVSLNGYGEHDIQGIGDKHV
                     TWIHNVMNTDAVVLVDDTDSKKILKDFTDESVFPYLVQTAGESNARMISHNFGISGVA
                     NLIAAIKTARFYNLGEDENIMIIATDSIGRYRSVMQEMPAITIESAVRDIERILLKQD
                     TCGVYEGTRENRMRWHNLKYFTWVEQQNKTVAQLNAQKEQAYWREQQEKTFETDKQIK
                     EYRKSHEKVLRNIMEY"
BASE COUNT         1464 a          942 c          928 g         1332 t
ORIGIN      
        1 cggcaggtga agcattgcag atattgccgc ctatcgttgc caagtttctt atggacgggc
       61 atgacataca ggatacaacg tccctgaaaa ctccccaaag caaagagctt tcaaggatat
      121 cactctgtgt tgcgcatgcg ccgatatgcg ttatgccatc tttaacagaa atttcatgaa
      181 gctcttcaat tttttgaagc ataagaagat catgctcatc acttaacaga aattgttttt
      241 taagcataag gtcggttcca cctgaaagta ccgtacactt caactctgat ctcagtaaaa
      301 gagcttggtc aagtgtaaca ggagaaaata ttctcaaagt tatccccccg agaccatatt
      361 ttttttataa tattcaaaag caagctctac tcccttaaag atgctgttat atccagtaca
      421 tctgcacagg tttccggaaa gatgttccat gagcatatcc ctggagggct taatattcct
      481 tttgaacatg tcatagcata cgattactat tcctggtgta caaaagccgc actggacgct
      541 tcccgcatcc gaaaagcctt tttttatggc ttgatactcc gcagtatcaa gtattccgct
      601 gagggtaaga atttttttat cctgtgccat aacaacagga tatatgcaag aatgcactgt
      661 aactccttct atcagaactg cgcaggctcc acattcacct tctttgcagc cctgtttgac
      721 atcagtctgg ttaaagtcat ttctcaggac atccgcaaga cttcttaaag gatctgtatc
      781 aacagtacaa aatatatggt taaggaaaaa attaatcttc attttcaatg acctccatga
      841 gatattctgc tgttaacggg agtttgcata ccggcttttt tacagcgtca agaacagccc
      901 cggcgactgc cggtcctact cccaaaaacg gtatttctcc ggcgcctttt gctccgaaag
      961 gcccgttttc ataggggttt ataatataat ccaaagatat ttcaacactg tctttagaag
     1021 aaggaataat ataatcagtt agattttttt gaaggatttt cccatccttc atactcataa
     1081 cctcgcaaga gctgtaaccg actccctgaa gaataccgcc ttctatctgg ccgtgaagag
     1141 tgtttgtatc tatcggtatg cctatatcca ttaccgtcca tattttttct atgctcagct
     1201 cataagtcag cgtatcgacg gataccaaag caatatcggc tccccatgag taagaaggat
     1261 aagcatcacc ctttaaaagc tcgttgtccc attcaagcca ggatggctgt tgataatcag
     1321 cttgaacctc aactataccc tcttcatccc atcgttgttt aagctcttca catgccttca
     1381 caagaagtcc tcctacgatc atagtgctgc gtgaagctac cgtaggtcct gaatccggaa
     1441 ccttatcagt atcaacagga gcgtatatta ctttagaaat atctatccca aggcttgaag
     1501 caactatttt acgcataacg gttccaagtc cctgacccat ctcaacgttt gatataaaga
     1561 taccgacggt gccgtcatga tttttcttca ataaggcacg tgcctttatt acttgctgtt
     1621 ctccgcttcc tgtaaaaccg catccgtgaa gaaaaaccga aaaaccaaca cctattttta
     1681 tgtgatcctc agagtttttt tcagaacaaa gttttttata atcagccatc tcttttactc
     1741 tgtttataat ttcagtaagt tttaaagggc ctcggaatgt tccgcctgta gtagttttat
     1801 ctccagtttt gacgatgtaa atgcttttaa aagcaacaac atcttggtgg agcataaatg
     1861 caagagcact catatgagtt tctatggcaa atatagcctg tggaccccca aaacctctaa
     1921 aagccccggt aggaacagtg ttggtagcgt aagcttttcc ttttataata aagttttcaa
     1981 agttatatac cccagtactg caaaacatag cccgttgaag gactacggac gatagtccgg
     2041 cgtaagcacc tgcattcagt tttatgtcaa tatcaacggc agttattttg tgattgaggt
     2101 ccagtgcggt ttttacagta ataacggaag gatgtctctt tgtggttgaa ataatgtctt
     2161 cttccctttc atagacaagt tttacgggtt tgccagattt aaaagccgca aatgcagcat
     2221 ggccagcaat tatagatgga aattcttctt ttcctccaaa agctccgccg gtagctgtct
     2281 ggactatgcg gattctttct ccgccccagc caaaagcttt ttccaaagca tgcttaatat
     2341 agtacgggca ttgcattgaa ccatacacgg ttatggttct gttttcataa atggctatca
     2401 ttccctgagg ttccatgtaa acgtgttcct gatatccagt ttcataagtt ctttcaacta
     2461 ctttttctgc agtagcgaaa gcttttttag catttcctct gcataactca tactcagcaa
     2521 aacagttgtc caaaccatat atgggtataa tatccttccg gagcgaatct tcataagaaa
     2581 gaacaggggt aagatcttcg taatcaataa cgaccttttc agccagttcg gctactttat
     2641 ggggatcggt tcccacaata agcattatgg gctctcctat aaagttcact tcgccgtttg
     2701 caaaaaaagg ctgatcatca agtatttctt ttactatgtt ggatgccgga atatctttat
     2761 agtcaactgt aaagtatccg tcgtcaagat aaggaaccgt aatattttta atttttgccc
     2821 gtgtcctttg agaacggacg gtcttggcat aaagcatgtc cttaaaagtc aaatcgtcaa
     2881 catacatggc cgcagcagaa attttttcct caaaatcatt tcttaaaaca ggatcagata
     2941 tacttgcaga catgccgcac ccccgtttac aaaccgcaaa aataagtaat ataataataa
     3001 catatttttt atataaaacc aaatactgta attaaaaaaa gaactcaaat attaattaaa
     3061 tacatagttt ataactataa gatacttaat ttatcttttt tatgactaag atatgaaaag
     3121 aagttaaaaa aattcatttt tgtcctttgc ttttatgaga aaaagtaaca taatattaat
     3181 actgaataat acttttcaaa ttctaaatac gggggtgaaa aatggaaaag attcctgtga
     3241 tagggccaac ctttgaagag atgcttcatc catggaagat agaagaaaca ataaggcgaa
     3301 cagcatggag aaaaagaacg gaagacccat tgcatcctat aaatctttac aacataacgt
     3361 ggagagatat aaataatcac ttctattttt ttgaggttcc gcatgagctt acaggtataa
     3421 aagcaaatat tgtagtaatg tatgcaaaag attttccgtc tgaaagccat aaagtaggag
     3481 caacgtatgc tattcttgct gaaaagacag ttaccgggga agtagatccg gccgttcaca
     3541 cccttgtatg gccctcgaca gggaattacg ggattggcgg tgcgtgggta gggtcacgca
     3601 tgaactatga ttccatagta cttctcccgg aaaagatgag ccgggaaagg tttgaaatca
     3661 taaggtcata tggtgcaaaa gcagtagcta caccaggctg tgagtccaat gtgaaggaaa
     3721 tatacgataa aagtaaagag ctctacaact cagacaggga acatgtgaga gttctgaacc
     3781 agtttgagga gtttggcaac taccgtttcc actggtatgt aaccggaaac tccatgattg
     3841 aactggtaag ggaaaataag attggaaatc agaaaattgc cgctgtagtt cttggagtag
     3901 gatcagcggg aactcttgca agtgctgata gggtaaagca ggaatttgca gattgtaaat
     3961 gcgttgcgat ggagcccgta cagtgtccca ccgtatcgct taatggatat ggcgagcacg
     4021 atatccaggg aataggagac aagcacgtaa cctggataca taatgtaatg aatactgatg
     4081 ctgtggtttt agttgatgat acagacagca aaaaaatact caaggacttt acagatgagt
     4141 ctgtttttcc atatcttgtg cagacagcag gtgagtcaaa tgcccgcatg atatcgcata
     4201 actttggaat atcaggcgtt gcaaacctta ttgcagctat aaagacagca aggttttaca
     4261 acttaggaga agatgaaaat atcatgataa tagcaaccga cagtataggc aggtaccgtt
     4321 cagtaatgca ggaaatgccg gctataacaa ttgaaagtgc agtaagagat atagaaagga
     4381 tactgctcaa gcaggatacc tgcggagtat atgaaggaac aagggagaac cgcatgagat
     4441 ggcataatct taagtacttt acatgggttg aacagcagaa caaaacagtt gcccagttaa
     4501 atgcccagaa agagcaagct tattggcggg agcaacagga gaaaactttt gaaacagata
     4561 agcagataaa agagtacaga aaatcacatg aaaaggttct cagaaatata atggaatatt
     4621 aaggaggagt aagatgaatc ctgtgatgca aagagcaaaa gagttg
//
LOCUS       DAOSHR010000158         4636 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_16590_length_4636_cov_4.838463, whole genome shotgun sequence.
ACCESSION   DAOSHR010000158 DAOSHR010000000
VERSION     DAOSHR010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 4636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4636
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16590_length_4636_cov_4.838463"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..178)
                     /locus_tag="PLU28_08420"
     CDS             complement(<1..178)
                     /locus_tag="PLU28_08420"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP synthase (glutamine-hydrolyzing)"
                     /protein_id="HOG35119.1"
                     /translation="MKKLLIIDFGSQYTQLLARRVREIGAYSEVVQHDEKLDLSEVQG
                     IILSGGPKSIYDTDA"
     gene            complement(156..>1352)
                     /locus_tag="PLU28_08425"
     CDS             complement(156..>1352)
                     /locus_tag="PLU28_08425"
                     /inference="COORDINATES: protein motif:HMM:NF017452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOG35120.1"
                     /translation="QRGEPAIVDKFARTQMALSYGADVVFELPFVYCVQDAGGFACGS
                     VGVLHRTGAVTDIVFGSESSDIGILNEISGILSSQPQEYTIILKKYLKEGLSFPNARK
                     YAILDYLSSQSYPIENIQLLEKSNDILGLEYLNYLKQYGSSIQAHCIARQGSSYNDET
                     VTNHLASATAVRNIIKEENIEFLKELIPEKSFEIILNEFKKGKGPVFFDSMREIILSK
                     IRLMNKNDLLKIYGIKEGLEDRFYNYSRRSMDLECLLKNLKTKRFTYSRLKRALVHIL
                     FNLSEKEIKTYNSQGPQYLRVLGFNTKGKELLAHIKKKSRYPLIPTASQYYQIYKYLL
                     KNQNDPDKKYDSDPIIYMDQINRDFDASNIYSLLYPEKEHAMFEPDKKNQIIIMEEKD
                     EKTSHY"
     assembly_gap    1353..1452
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1453..1633)
                     /locus_tag="PLU28_08430"
     CDS             complement(<1453..1633)
                     /locus_tag="PLU28_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOG35121.1"
                     /translation="MRVLGLIVEYNPFHNGHLYHIKKSVELINPDYTVAVMSGNFVQR
                     GEPAIVDKFARTQMAL"
     gene            complement(1638..2735)
                     /gene="ychF"
                     /locus_tag="PLU28_08435"
     CDS             complement(1638..2735)
                     /gene="ychF"
                     /locus_tag="PLU28_08435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HOG35122.1"
                     /translation="MLNVGFVGLANVGKSTLFNALSKQHVPAENYPFCTINPNIAIVE
                     YNDERLDLMTKILNSEKKVNPSIEFVDIAGLVKGASKGEGLGNLFLENIRNVDCIAHV
                     IRIFSDPNVTHVNGKIDPIADADIVNLELIAKDFETVEKRIEKTSKIARVGDKEARTE
                     TEILSELKEHLNSEKPSITFLKGERTEKQQKIIESLFLLTEKPVIYVANIDESPNETT
                     NEKIKAFREYAFSKGAQFTEIHAKLHSDLSDLDEQEQKVFLNEYGIEKSALDYFILKC
                     KQALNLVTFITGNEKESKSWNVVNGTNALQAAGKIHSDIQEGFIKAEVINFEKILEIP
                     DMKKLKELGYINAEGKDYLIKEGDYIYFHFR"
     gene            2870..3991
                     /locus_tag="PLU28_08440"
     CDS             2870..3991
                     /locus_tag="PLU28_08440"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HOG35123.1"
                     /translation="MSYAFSYFIPTRVFFGRDCLNNNYATLSGLGEKALIISGRKSAK
                     ACGAYDEIVEVLNKLNIESVLYDQVEENPSFSSVQKVVEFGLENDADFVIGIGGGSPI
                     DAAKAVAVLIANPFMKIEELFDGDFDIALPIVAIPTTSGTGSEVTEFSVLNSPENRKK
                     SFKSDVIFPDISFIDPKYTLDLPTELTITTACDALSHSIEGSLSLKSTILTDLYAAKA
                     IELIKENLPKLVNSSLVLSEEERYECRQALSLASMLGGMVISQTGTVLPHSFGYNMTT
                     FYGIRHGVATIIFLPSVLRKAERTNPEKLRVFKESFKSVDKFDDFLEKLGVYKNIPKL
                     SEKDLVDFSQFVAKSMHVSVTPGNITYEDIISIYKEVCG"
     gene            3988..4227
                     /locus_tag="PLU28_08445"
     CDS             3988..4227
                     /locus_tag="PLU28_08445"
                     /inference="COORDINATES: protein motif:HMM:NF036808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35124.1"
                     /translation="MIAHLRKDIKSRVLNGHPWIYENEIESVESGVKEGDILSVFFGK
                     NFIGKGYYNPNSVIRIRILTRKNEEIKFIFFIKKY"
     gene            4401..>4636
                     /locus_tag="PLU28_08450"
     CDS             4401..>4636
                     /locus_tag="PLU28_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HOG35125.1"
                     /translation="MKILNPKGIYEKDDERSAQKEKFVPYQGWVYANGPELIEFAIEG
                     VKFICDVQGQKTGFFLDQRINAQNIAKYASNKKV"
BASE COUNT         1316 a          768 c          887 g         1565 t
ORIGIN      
        1 gtgcatcagt gtcatatatg cttttgggtc ctccggaaag tataattccc tgtacttccg
       61 ataaatcaag tttttcatcg tgttgaacaa cctcagagta tgcgcctatt tctctcactc
      121 ttctggccaa aagttgtgtg tactgtgaac cgaaatcaat aatgagaagt tttttcatct
      181 ttttcctcca tgataatgat ctgatttttt ttgtctggtt cgaacatagc atgctctttt
      241 tctgggtaaa gcaggctgta tatatttgaa gcatcaaaat ccctgtttat ttgatccata
      301 tagataatgg gatcagagtc gtattttttg tccggatcat tttgattttt gagcagatat
      361 ttatatatct gatagtactg tgaagctgtc ggtataaggg ggtaacggga ttttttctta
      421 atatgtgcaa gcagttcctt gccctttgta ttaaagccta agactcttaa atattgtggt
      481 ccttgtgaat tataggtttt aatttccttt tctgaaaggt taaacaaaat atgtaccagt
      541 gctctcttta agcggctata tgtaaagcgt ttagttttta aatttttaag aaggcattcg
      601 agatccatag atcttctgct atagttataa aacctatctt ccaatccttc ttttatgccg
      661 tatatcttca aaaggtcatt tttgttcata agtctgattt tgctcagtat gatttcacgc
      721 atagaatcga aaaaaacagg tccttttcct tttttaaatt cgttcagaat tatttcaaag
      781 gatttttcag gaataagttc ttttagaaat tctatatttt cttctttgat gatatttctg
      841 acagctgttg cgcttgcaag gtgattggta actgtttcat cgttgtatga tgaaccttgt
      901 ctggcaatac agtgagcctg tattgaggaa ccgtactgtt ttagataatt aagatattca
      961 agtccaagga tatcgtttga tttttccaac agttgtatgt tttcaatagg ataactttgg
     1021 ctggaaagat aatcaagtat ggcgtatttt ctggcatttg gaaatgaaag tccttctttg
     1081 agatattttt ttaggataat ggtgtactct tgaggctgtg aactcagtat tccggaaatc
     1141 tcatttaaaa ttcctatatc agatgattca cttccgaaaa ctatgtctgt tacagctcct
     1201 gttctgtgca atactcctac ggagccgcat gcaaaccccc cggcatcctg tacgcaataa
     1261 acaaaaggga gttcaaatac aacatctgca ccgtaagaaa gagccatctg ggtacgtgca
     1321 aacttgtcga caatagccgg ctctcccctc tgnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnaaagagcc atctgggtac gtgcaaactt gtcgacaata gccggctctc
     1501 ccctctgaac aaagttgccg ctcatcactg caactgtgta atcagggttt ataagttcta
     1561 ctgatttttt aatatgataa agatgaccgt tgtgaaatgg attatactca actataagac
     1621 ccagaactct cacggtttca ccggaaatgg aagtatatgt aatcgccttc ttttattaaa
     1681 taatcttttc cttctgcgtt tatatagcca agttctttta attttttcat atcgggaatt
     1741 tcgagtattt tttcaaaatt tataacttcg gcttttataa agccttcctg tatatctgag
     1801 tgtatcttgc cggctgcctg cagagcgttt gttccattca cgacattcca tgatttagat
     1861 tctttttcat tgcctgtgat aaatgtgaca agattcagag cctgtttgca ctttaaaatg
     1921 aaatagtcaa gtgcgctttt ttctatgccg tactcattta aaaacacttt ctgttcctgt
     1981 tcatcaagat cagaaaggtc ggaatgaagc ttagcatgaa tttcagtaaa ctgtgcacct
     2041 ttagaaaagg catactctct gaatgcttta attttttcat tggttgtttc attgggagat
     2101 tcatcaatat ttgcgacata tattacaggc ttttcggtaa gaagaaatag ggattcgatt
     2161 atcttttgct gtttttcggt tctttctccc tttaggaagg tgatggaagg tttttcgctg
     2221 ttaagatgtt ctttcagttc ggaaagtatt tctgtttcgg tccgcgcttc tttgtctccg
     2281 actctggcaa tcttggaagt tttttcaatt cttttttcta ctgtttcaaa gtcttttgct
     2341 attagttcaa gatttactat atctgcatcc gctatgggat caatttttcc attaacgtgt
     2401 gttacgttag ggtctgaaaa aattcttata acatgagcaa tacagtcaac attacggatg
     2461 ttttccagaa acagattgcc tagtccttct cctttgcttg ctccttttac aagacctgca
     2521 atatcaacaa attcaatgct gggatttact ttcttttctg aatttagtat tttagtcatt
     2581 aagtccaaac gttcatcgtt gtactcaaca atggcaatgt ttgggtttat tgtgcagaaa
     2641 ggatagtttt cagcaggaac atgctgtttg gaaagagcat tgaaaagggt tgacttccct
     2701 acgttggcaa gacctacaaa tcctacgttt agcataatgt cctccttgaa aatctttttg
     2761 taatcatttt tccgtatcaa ttataacagt agaatttatc tttttctgta aaatttctta
     2821 aacaaaatca tgttaaaata gattaaacta tataaggggg gagtatcgaa tgtcgtatgc
     2881 cttttcttat tttattccga caagagtttt tttcggcagg gactgtctta ataataacta
     2941 tgcaacttta agcggtttag gggagaaagc tcttataata agcggacgaa aatcagctaa
     3001 agcctgcggg gcttatgatg aaatcgttga agttctaaat aagctgaata tagaatctgt
     3061 tttgtatgat caagttgaag aaaatccgtc tttttcttcg gttcagaaag ttgtagagtt
     3121 tgggcttgaa aatgatgcag actttgtaat aggtattgga ggaggaagtc ctatcgatgc
     3181 agcaaaagcc gttgcggtat taattgcaaa tccttttatg aaaatagaag agctgttcga
     3241 tggtgatttt gacatagcgc ttccgatagt tgcaattcct accaccagtg gaacaggaag
     3301 cgaagtaact gaattttccg ttttgaactc tcctgaaaat aggaaaaaaa gctttaaaag
     3361 cgatgtaatt tttcctgata taagctttat agatcctaaa tatactcttg atcttccgac
     3421 tgaacttaca ataacgactg cctgtgatgc tctttcacac agcatagaag gttctttatc
     3481 tctgaaatcc actatactta ctgatcttta tgcggcaaag gctattgagt taataaaaga
     3541 aaatcttcct aagcttgtga actcttcctt ggttttatcc gaagaagaaa gatatgaatg
     3601 caggcaggct ctatctctgg catcgatgct tggaggtatg gttatatctc agacagggac
     3661 ggttttgcct cattcgttcg gttacaacat gacaactttt tacgggataa gacacggagt
     3721 tgcgactata atctttttgc ctagtgtttt gcgtaaggct gaaaggacta atcctgaaaa
     3781 gttgagagtc tttaaggaaa gttttaaatc agtagataag tttgatgatt ttcttgaaaa
     3841 attgggagtt tacaagaaca ttccaaagct ttctgaaaag gatttagtcg atttttcaca
     3901 gtttgtggca aaatcgatgc atgtctccgt tactccgggt aatataacgt atgaagatat
     3961 aatatccata tataaggagg tttgtggatg attgctcatt taaggaaaga tataaagtcg
     4021 agggtgctta acggtcatcc atggatttat gaaaacgaga tagagtctgt agaatctggg
     4081 gtaaaagaag gagacatact cagcgttttt tttggtaaaa attttatagg caaaggctac
     4141 tataatccta attctgttat aagaataagg attctgacca gaaaaaacga ggaaatcaaa
     4201 tttatttttt ttataaaaaa atactaaagg cttatgaata cagaagatca ctttttccgg
     4261 gatcgaacac ttacagactt gtctttgcag agtctgacgg tcttcctggt ctggttgtag
     4321 acagatttga cgactacgtt atattccaga taaatacttt gggaatatac gaacataaag
     4381 atgatattat agagagtata gtgaaaatac ttaatcccaa gggaatatat gaaaaggatg
     4441 atgaaaggag cgcccagaaa gaaaagtttg taccgtatca aggctgggtg tacgcaaacg
     4501 gtccagaatt gattgaattt gcaatcgaag gtgttaagtt catatgtgat gtgcagggtc
     4561 agaagaccgg tttttttctt gatcagcgca tcaatgcaca gaatatagcc aagtatgcat
     4621 cgaacaaaaa agtgtt
//
LOCUS       DAOSHR010000159         4601 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_16772_length_4601_cov_4.489221, whole genome shotgun sequence.
ACCESSION   DAOSHR010000159 DAOSHR010000000
VERSION     DAOSHR010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 4601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4601
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16772_length_4601_cov_4.489221"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            215..973
                     /locus_tag="PLU28_08455"
     CDS             215..973
                     /locus_tag="PLU28_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35126.1"
                     /translation="MLLCEKLNFIFEFRNKIKDIHPLLNKCFPVVVSSVDKVDIYDVT
                     PEEKYTLVKTDKNMFGHIPHIRASFPFESYGNRPVCIITEDVFDEPNGYAVIFHEFIH
                     CQQYELCETRLKKRLSVYNQSMLEKNYMWELNYDFPYSSIEFMSYCNTDASENNLLKT
                     LAEKRRLIKNNYGIANYEYMTWQEWKEGFAEYIETKIIEKTGKNVEQSDSLSMSRVCL
                     YKTGARFIGALKTKYHNCDENIESLFEMIYENNN"
     assembly_gap    1169..1178
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1179..1516)
                     /locus_tag="PLU28_08460"
     CDS             complement(<1179..1516)
                     /locus_tag="PLU28_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35127.1"
                     /translation="MKLIKPCEELAAEYLEICIEYSHENNSRHGGINTLEKAVQRIKK
                     DICLEDSNNHSEEVKTSTRWLVDQKNMLIGTCRLRTELRNVESNLDGHIGYDIRPSCR
                     KMGYGKVILKL"
     gene            1639..2499
                     /locus_tag="PLU28_08465"
     CDS             1639..2499
                     /locus_tag="PLU28_08465"
                     /inference="COORDINATES: protein motif:HMM:NF014749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35128.1"
                     /translation="MNFIRVTDMTIQDIKRIFTVADSLRSGGAITSLSGRTAVMFFPE
                     SSIRTRITFEKGLHCMGITPILFPSSTLDKREDIADVTGYIENWADLLIIRHPSIEKI
                     ESISKYSHIPVINAMTSQNHPCEILSDIYSIRDRNTNYSNLIYTFVGENSNILHSWIE
                     ASEVFGFRLNHVSLPENEVDDAGKNYFFFNDLNDIISQTDVLLTDPLTEKYRNEEYYR
                     KFQITLKLLQKGKNDLLFNPCPPFYRGEEVSSDAISSEYFVGYSFKKNLLYVQQAIMA
                     YVSETVGGLL"
     gene            2496..3209
                     /locus_tag="PLU28_08470"
     CDS             2496..3209
                     /locus_tag="PLU28_08470"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOG35129.1"
                     /translation="MRKLETKYAELSTVKLAYSEYGNGENLLLLHGNSANKSIFGKYQ
                     TEYLKNFHTYAIDSRGHGKSISVDEKYTIKQYSEDVIEFCRYLNIDKAFVLGYSDGGN
                     ISLFLAAQDPKRFSKIYAISPNYLACGTTDSSLSFLKKIYRMLNFMNRIGLNTKKAIM
                     RFDLMLNDIGLSKQQLQSISTQVRIAYAQKDMIKHEHIIEISQLIPDASLKKIDGCTH
                     ISEFKSSELISDMQHFFAV"
     gene            complement(3266..3823)
                     /locus_tag="PLU28_08475"
     CDS             complement(3266..3823)
                     /locus_tag="PLU28_08475"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOG35130.1"
                     /translation="MYAYEAIQNSLDFIECHLKDSLNAEDIAAETGLSVYYFQHLFSR
                     LIKKTLNEYIKLRRLAKASEILKDKEIRIIDVAVEYGFSDHANFTRAFREAYGITPEE
                     YRSFPVVLNHFIKPDLTLDYKDTEENVPLIADGIVIEIIRMCGKGLGEYIVCCVEAEN
                     FEEMIGSAIFKAYSFIGKWIKKHEN"
     gene            complement(4059..>4601)
                     /locus_tag="PLU28_08480"
     CDS             complement(4059..>4601)
                     /locus_tag="PLU28_08480"
                     /inference="COORDINATES: protein motif:HMM:NF024769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HOG35131.1"
                     /translation="FPNRTAFREWLDKNNSKNEGVWLLFGKDGGPITLSASQALEEAL
                     CYGWIDGQLKNLDEKTYKKYFSRRRADSKWSEKNKALVAKLEQQGKMTDYGRKKVEEA
                     KKNGQWDKPKVLPVTEEQIASLSEVLKKYEPAYTNFLSMSPSVKKTYTRAYYDAKTDS
                     GRTSRILWMVERLNKNLKPM"
BASE COUNT         1441 a          868 c          760 g         1522 t
ORIGIN      
        1 gattgaagga atggcatgtg aaggcggatg tatagccggt cccggtgtta tagtgaatcc
       61 taaagtttca tcttcaaacg ttaaaaaaat tgcaaatata aaataaagtg tagaggcaat
      121 gcctctacac tttatttttt taccggtata aattgtttaa ctctgttcaa gtatgataga
      181 attgaatcat ctaaaacaat gaaaggagcc gcttatgttg ctttgcgaaa aattgaactt
      241 tatatttgaa ttccgcaata agataaagga tatacatcct ttattaaaca aatgctttcc
      301 ggtagtagtt tctagtgttg ataaagttga tatatacgat gttactcctg aagaaaaata
      361 tacacttgtt aaaactgata aaaatatgtt cggtcacata cctcatattc gtgcttcatt
      421 cccttttgaa tcttacggta acagacctgt atgtattatt actgaagatg tctttgatga
      481 accgaacggt tatgccgtta ttttccatga gttcatacac tgccagcagt atgagttgtg
      541 cgaaacccga ctgaaaaaaa gactttctgt ttataaccaa tcaatgcttg aaaagaatta
      601 tatgtgggag cttaactatg attttccgta cagttcgata gagttcatga gctactgtaa
      661 cactgatgct tcagaaaata atctacttaa aactcttgct gaaaaaagaa gacttataaa
      721 aaataattat ggaattgcca attatgagta tatgacatgg caggaatgga aagaaggctt
      781 tgccgagtat attgaaacca agatcattga aaaaaccgga aaaaacgttg aacaatctga
      841 ttcactatct atgtcacggg tctgcttgta taaaaccggt gcaagattta ttggtgcctt
      901 aaaaaccaag taccacaatt gcgatgaaaa tatagaaagt ttgtttgaaa tgatctatga
      961 aaataacaac tgattaaaac actaaggtac acttaaatac tttacttttc cagattgatc
     1021 cagtaccttc tcacattctt gtgatcagaa tcatcaaaaa ctgttctttc ataaattccg
     1081 tcattgcttt ctattatctt tcgtgagcct atgttatctt catcacaagt tataagaata
     1141 ttttttagtc catacagctg tatttcatnn nnnnnnnnag ttttagaata acttttccgt
     1201 accccatttt tctgcaggaa ggacgaatat cgtatcctat gtgtccgtcc aggttagact
     1261 caacattcct tagttcagtc ctaagtctgc atgtcccaat gagcatattt ttttgatcaa
     1321 ctaaccatct ggttgaagtt ttaacttctt ctgaatggtt attgctatct tcaagacaga
     1381 tatccttttt tattctttgt actgcctttt ccagagtatt tataccaccg tgccttgaat
     1441 tattttcatg cgaatattca atacagatct ccaggtattc agccgctagt tcttcacatg
     1501 gttttataag cttcaacagt actcctcctc tttttgaaca gcattaatat aatattagca
     1561 ctaaatccgg aattttaaaa aatatttgag gcttatgata aaattgaact actaaaattt
     1621 tttattacgg aggataagat gaattttata agagtcacag atatgaccat tcaggatatt
     1681 aaaagaatat tcacagtagc tgacagctta agaagtggtg gggctataac cagtttatcc
     1741 ggcaggactg ccgtaatgtt ttttcctgaa tccagcatac gaacacgcat aaccttcgaa
     1801 aaaggtctgc actgcatggg aattactcct atactctttc cttcttctac tttggataaa
     1861 agagaagata tcgcagatgt caccggatac atagaaaact gggctgatct gctcataata
     1921 aggcatcctt ctattgaaaa aatcgaaagt atctcaaaat actctcacat acctgttata
     1981 aatgctatga cctcacaaaa ccatccgtgt gagattcttt ccgatattta ttcaatacga
     2041 gataggaata ccaattatag taatttgata tatacttttg taggtgaaaa ttcaaatatt
     2101 cttcattctt ggattgaagc ctcagaagtt tttggtttca gactgaacca tgtttccctg
     2161 ccggaaaacg aagttgatga tgcaggaaaa aactacttct tctttaatga tcttaacgat
     2221 atcataagtc agaccgatgt gcttcttacc gatccgctta cagaaaagta caggaacgaa
     2281 gaatactacc gtaaattcca gattacactc aagcttctgc aaaaaggaaa aaacgatctt
     2341 ttatttaacc catgcccacc tttctataga ggtgaggaag tttctagtga tgctataagt
     2401 tcagaatatt ttgtaggata cagcttcaaa aaaaatctgc tctatgtaca gcaggcaatc
     2461 atggcatatg tatctgaaac agttggaggt ttattatgag aaaacttgaa accaagtacg
     2521 ctgagcttag tactgttaaa ctagcttatt ctgagtatgg aaacggggaa aatcttctgt
     2581 tgctgcacgg taattctgcg aataaaagta ttttcggtaa gtatcagact gaatatttga
     2641 aaaattttca tacttacgca atcgacagca gagggcacgg aaagagcata tctgttgatg
     2701 aaaagtacac aataaagcag tacagcgaag atgtcatcga attttgcaga tacttaaata
     2761 ttgacaaagc ttttgttttg ggatacagcg acggtggaaa tatttcatta tttcttgctg
     2821 cacaagaccc gaaaagattc tctaaaatct atgccatatc ccctaattac cttgcctgcg
     2881 gtacaaccga cagctcttta agttttttga agaaaattta ccgcatgctc aacttcatga
     2941 acagaattgg gcttaataca aaaaaagcta taatgcggtt cgatttaatg cttaatgata
     3001 tcggtttatc caagcaacag cttcagagta taagcacaca ggttcgaatt gcttatgcgc
     3061 aaaaggatat gataaaacat gagcatataa tagaaataag ccaattaatt ccggatgcat
     3121 ccttaaaaaa aattgacggc tgtactcata tcagtgagtt taaaagctct gagcttatct
     3181 ctgatatgca gcatttcttt gcagtgtgac tcacaaacct ataagatcgg tcagattggt
     3241 ttttgctgtt tacaaatagt aaagcctagt tttcatgttt ttttatccat tttcctataa
     3301 atgagtatgc cttaaagatt gctgagccta tcatttcttc aaagttctcc gcttctacac
     3361 agcatacaat atattcgcct aatccttttc cacacatacg gataatctct ataacaattc
     3421 catcggcaat taacggaacg ttttcttcag tatccttata atccagtgta agatcaggtt
     3481 ttataaaatg attaagaact acgggaaagc ttcggtactc ttctggggta attccatatg
     3541 cttctctaaa ggcacgtgta aagtttgcat gatctgaaaa tccatattcg acagccacat
     3601 ctattattcg tatctcttta tctttaagta tttcagaagc ttttgcgagt cttctcagtt
     3661 taatgtattc gttcaatgtt ttttttatta aacggctgaa cagatgctga aaatagtata
     3721 ctgacagtcc tgtttcagca gcaatatctt ctgcatttaa gctatccttt agatgacatt
     3781 ctatgaaatc cagagaattt tgtattgctt cgtatgcata caccttacca cctccctaga
     3841 gtatagaata ccacatctat tactttttcg cttgtcaccc aatgctcttt taaagtttct
     3901 gctgaaaaaa ctgcacaagc attatcttgt gcagtagttg atacatataa gtttttatag
     3961 taaagctatt ttttctccaa actctacagc tgctttttga ccattattat caggattcca
     4021 ttggtttttt agtccctcat ctatgatact acttagaact acatcggctt caaattttta
     4081 tttaaccttt caaccatcca gagtatacgg cttgtgcgcc ccgagtcagt tttagcatca
     4141 taatatgccc gtgtatatgt cttttttaca gatggcgaca ttgataaaaa gttcgtataa
     4201 gccggttcat atttttttaa cacctcagat aaagaagcta tttgttcctc cgtaacaggc
     4261 agaactttag gcttgtccca ctgcccgttt tttttggctt cctcaacttt ctttctacca
     4321 taatctgtca tcttcccctg ttgttcaagt tttgcaacta aagccttatt cttttcagac
     4381 catttactat cggcacgccg cctggaaaaa tattttttat atgttttttc atcaagattt
     4441 ttaagttgac cgtcaatcca accatagcat aaagcttctt ccaaagcctg gctggcagag
     4501 agcgtaatag gtccgccatc tttaccaaag agcaaccata ctccttcatt ttttgaatta
     4561 ttcttatcca accattcacg aaatgccgtt ctatttggaa a
//
LOCUS       DAOSHR010000160         4462 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_17594_length_4462_cov_4.894032, whole genome shotgun sequence.
ACCESSION   DAOSHR010000160 DAOSHR010000000
VERSION     DAOSHR010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 4462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4462
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17594_length_4462_cov_4.894032"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="PLU28_08485"
     CDS             <1..347
                     /locus_tag="PLU28_08485"
                     /inference="COORDINATES: protein motif:HMM:NF013056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HOG35132.1"
                     /translation="IDGKNAPRNSLLRTVIFSPLAAAIPSAATAPALIFVGSLMVMSL
                     KEIEWEDTTEGLPAFITMVSMPLTYSIATGITLGMIFYPIIKLFTGKYKQVNVMTWIL
                     AALFILYLIFLH"
     gene            complement(391..1455)
                     /gene="glpX"
                     /locus_tag="PLU28_08490"
     CDS             complement(391..1455)
                     /gene="glpX"
                     /locus_tag="PLU28_08490"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HOG35133.1"
                     /translation="MKQLNYPEITLDLVRVTEAASLTSSLYLGFGNKEAVDGAAVDAM
                     RGMLDYMDMKGIIVIGEGEKDEAPMLYIGEHVGCWADDSPELDIAVDPVDGTRLVSYG
                     LPNAVSVMVAAERGKIAYLPTFYSYKLAVGPELKGRLDINASIRENLRIAAAALGVPI
                     SEITVVVLNRDRHNPIIEEIRKVGARIKLIGDGDIAAAIATAMPDTGVDIYIGIGGSP
                     EAILAAAAMKTLNGEMQVKLWAKDGAERERFEKEGWNFDKIYYTDDLIGGDDVIFAAT
                     GVTDGDFLKGVKFLKGTAITESLVMRSSSATIRKIQTHHDLSRKTIRIKSLNGDEKIL
                     NINKRSISNPNLGFSNMMFR"
     gene            1641..3041
                     /locus_tag="PLU28_08495"
     CDS             1641..3041
                     /locus_tag="PLU28_08495"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOG35134.1"
                     /translation="MDVKVLKDQLLRKKETVWKKRKIEEINEYCKSYAEFMDNSKTER
                     LSVKNIIALLEKNGYKSFESIKKTGKLPKNAKVYFSNRGKSLFAIRVKGDIKNGVNIV
                     GAHVDSPRLDLKPEPIIEDYEIAMAKTHYYGGIKKYQWLNIPLQMYGTIVKKDGSIID
                     ISIGDKPEDPIFVISDLLPHLDRREGDVKKVFEAEKLNILLGSVSVSYDEDIKEGVKL
                     NVLKILNEKYGIVEEDFISAEIEVVPAFNSRDVGLDRSLIASYGHDDRVCAYTALSAI
                     IEAEPKFKSSALIIADKEEIGSEGNTGSKNHYWIGILKELLSLQKEEVTLAIEETLRN
                     SVLLSADVSAAVDPNYKDVHDLTNAPKLGYGIVITKYTGSGGKGATNDANAELVGKIR
                     KILNDSNVTWQTGELGRTDLGGGGTIAKFFAEKGLDVIDAGVPLLGMHAPYEIASKAD
                     IYETYLAYKAFMESMD"
     gene            3038..3616
                     /locus_tag="PLU28_08500"
     CDS             3038..3616
                     /locus_tag="PLU28_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35135.1"
                     /translation="MKLYAGNPINAIEFMNDDLNLIISTSPWSFKDQGMAVIIIDKKI
                     KLYTGEQVYEINTESRFLNLNEYKLCFGFGHDCLNYDFFFMNWIKNTDLIILSINIEK
                     KENYEKFFDHIASNISIVKIPTLLLFCIDEKFFTAIFDETNSYKTEKNNYLLYCYTGI
                     LKKEKRLVLKDKHIRELTSSLKTIRKRIINNK"
     gene            3613..4305
                     /locus_tag="PLU28_08505"
     CDS             3613..4305
                     /locus_tag="PLU28_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35136.1"
                     /translation="MRSYFNKIRWHFLKKKVLKILEKIKTPDYDPQKLNIEIIRTTNL
                     RYILNSIKTGLNIGCMLFLIISGKELTDSELEVMAELFNTFGINFILSDKKNLYRIYE
                     NSVKKISEYDNIKLRSFFIKSFNVNFILFEDSISPAQIMNLKINKTDFIFFDNRISAV
                     TMKLFADSLFCFCTNGNEIVSPHMISNNQDTNMIYKSILSFDYTMLNSVKRNYITWDE
                     QIISEIEKNLNT"
BASE COUNT         1500 a          822 c          764 g         1376 t
ORIGIN      
        1 ctattgacgg aaaaaacgca ccgcggaatt ccctgctgag aactgttatt ttttcacctt
       61 tggctgctgc aattccttct gcagcaacag cacctgcact tatttttgta ggttcgctca
      121 tggttatgag tcttaaggaa atagaatggg aagacacgac agaagggctt ccagcattta
      181 ttaccatggt aagtatgcct ttgacctact ctatcgctac tggaattact ttgggaatga
      241 ttttttatcc tataataaaa ctgttcaccg gaaagtataa acaagtaaac gtaatgactt
      301 ggattttagc agctttgttt atactctacc ttatcttcct tcattaaaaa tttacttcca
      361 caaaatcatc tgattttgtg gaagtatttt ttatctgaac atcatattgg aaaaaccaag
      421 gttaggattg cttatacttc ttttgtttat attaagtatc ttctcgtccc cgttaagtga
      481 ttttatacgt atagtcttac gactcagatc atgatgagtt tgtatcttac gtatcgttgc
      541 actggagctt ctcattacca aagattctgt tatggcagtt cctttaagga actttactcc
      601 cttaagaaaa tctccatccg ttacccccgt tgctgcaaat attacgtcgt cgccacctat
      661 aagatcatca gtataataaa tcttatcaaa attccagcct tctttttcaa atcgttctct
      721 ttcagcaccg tctttggccc aaagttttac ctgcatctct ccgttaagag tcttcatagc
      781 cgctgcagca agtattgctt ccggagaacc tcctatacct atataaatat ctaccccggt
      841 atccggcatt gcagtggcaa ttgcagctgc aatatctcca tcacctatga gtttgatcct
      901 ggcaccgact tttcttattt cctctattat aggattatgt ctgtcacggt tgagaactac
      961 aactgttatt tcacttattg gcactccgag agcggcagcg gcgatacgaa gattttctct
     1021 tattgaagca ttgatatcaa ggcgaccttt cagttccggc ccgacggcaa gtttgtaaga
     1081 ataaaacgta ggaaggtaag ctatctttcc tctttcggct gcgaccataa ctgatactgc
     1141 attcggcaaa ccgtacgaaa caagtcttgt tccgtcgaca ggatccacag ctatatccag
     1201 ttcaggtgag tcgtcggccc agcatccaac atgttctcct atgtacaaca taggagcctc
     1261 atccttttct ccttcaccta ttacaatgat tcctttcata tccatgtaat caagcattcc
     1321 tctcatggca tctactgcag ctccatcaac agcctctttg ttcccaaaac caagataaag
     1381 actgcttgtt aatgatgcag cttctgttac tcttacaaga tccagagtga tctccggata
     1441 gttgagctgt ttcactttta aaaacctccc cttgatggta taattgtagt gagtacccaa
     1501 ataccaaact atattttcat gttgaaaaaa ataaacttcc ttttacaact taattataaa
     1561 ctaaaaactg ataaaactga aattaatatt tccaataaaa agatggttta accacaaaat
     1621 tagcccgggg gtgcaaataa atggatgtaa aagtattaaa agaccaatta ttaagaaaga
     1681 aagaaactgt atggaagaaa cgaaagattg aagaaataaa tgagtactgc aagagctatg
     1741 ctgagtttat ggataactca aaaactgaaa ggctatctgt aaaaaatatt atagctttat
     1801 tagaaaaaaa tggttataag tcctttgaat ctattaagaa aaccggtaaa ctccccaaaa
     1861 atgccaaagt atacttttct aatagaggaa aatctctttt tgccatacga gtaaaaggag
     1921 atattaaaaa cggagtaaat atagtggggg cacatgttga ttcaccacgc cttgatctta
     1981 aaccagagcc tattattgaa gattacgaga tcgctatggc taaaacacat tactacggtg
     2041 gaattaaaaa gtaccaatgg ctcaatattc ctcttcagat gtacggaact atagtcaaaa
     2101 aagatggctc aattattgat atttcaattg gagataagcc cgaagatccc atatttgtta
     2161 taagcgacct gcttcctcat cttgataggc gggaaggcga tgtaaaaaaa gtttttgaag
     2221 cagaaaaatt aaatatactt ttgggttcgg tttctgtaag ttatgacgaa gatattaaag
     2281 aaggagtcaa gcttaatgtg cttaaaatcc ttaatgaaaa gtacggtata gttgaagaag
     2341 attttattag tgcggaaata gaagttgttc cggctttcaa ttcaagagat gttgggcttg
     2401 acaggagtct tattgcttct tacgggcacg atgacagagt atgtgcctac actgctcttt
     2461 cagctattat tgaagctgaa ccaaagttta aatcctctgc gcttataatc gctgacaaag
     2521 aagaaatagg aagtgaagga aataccggat caaaaaatca ttactggata ggaatactaa
     2581 aggagcttct aagccttcaa aaagaagaag ttaccttagc cattgaagaa accttaagaa
     2641 attccgttct tttatcagct gatgtttctg cagcagttga tccaaactac aaagatgttc
     2701 acgaccttac caatgcccct aagttaggat acggcatagt aataaccaag tacactggca
     2761 gtggtggaaa aggagcaacc aacgatgcaa atgctgaact tgttggaaaa ataagaaaaa
     2821 ttcttaatga ctccaatgtt acatggcaga ctggagaact cggcagaaca gatcttggcg
     2881 gaggcggtac catagcaaaa ttctttgcag aaaaaggtct tgacgttatc gatgctggtg
     2941 ttccacttct tggaatgcat gcaccgtacg aaattgcttc aaaggctgat atatacgaaa
     3001 cttatctcgc ttacaaagct tttatggaga gtatggattg aagctttatg caggcaaccc
     3061 aataaatgcc attgaattta tgaatgatga tttaaacctt ataatcagca catcaccctg
     3121 gtcttttaaa gaccagggaa tggctgttat aataattgat aaaaaaataa agctttatac
     3181 cggagaacaa gtttatgaga taaataccga atcacgcttt cttaatctta atgaatacaa
     3241 gctctgcttc ggtttcggac acgactgttt gaactacgat tttttcttta tgaattggat
     3301 taaaaatact gatctaataa tactttctat caatattgaa aaaaaagaaa attatgaaaa
     3361 attctttgac catatagctt caaatataag cattgtgaaa atacctacct tgcttctatt
     3421 ctgcatcgat gaaaaatttt ttacagcaat ctttgatgag actaactctt acaaaactga
     3481 aaaaaacaac tatctgctct actgttacac cggaatactt aaaaaagaga aacgattagt
     3541 tcttaaagat aagcatatca gagaacttac gagcagtttg aagactatac gcaaaaggat
     3601 tataaataac aaatgagaag ctattttaat aaaataagat ggcacttcct aaaaaagaaa
     3661 gttctgaaaa ttctcgaaaa aatcaaaact ccagattatg acccacaaaa attgaacatt
     3721 gaaataataa gaaccaccaa cttaagatat attttaaatt ctataaaaac aggccttaac
     3781 atcggttgta tgcttttttt aataatatct ggaaaagaac ttacagatag cgagcttgag
     3841 gttatggcag aactttttaa cacttttgga atcaatttta ttttaagcga taaaaaaaat
     3901 ctctacagaa tatatgaaaa ttcagtaaaa aaaatatcag aatacgataa tataaaatta
     3961 cggtcttttt ttattaaatc atttaacgtt aactttatac tttttgaaga tagtatttct
     4021 ccagcacaga taatgaatct taaaataaat aaaacagatt ttattttttt tgacaaccgt
     4081 atttccgcag tcaccatgaa gctttttgcg gattcgcttt tttgtttctg tacaaacgga
     4141 aatgaaattg tctcacctca tatgatctcc aacaatcaag atactaatat gatttataaa
     4201 agtattcttt cattcgatta cacaatgctt aactctgtca aacgaaatta tattacgtgg
     4261 gatgaacaga ttatatcgga gattgaaaaa aatttaaata cctaattttc aatttaaaaa
     4321 cctgcggcat agccgcaggt tttttatcaa aactcatctc tcatgaatgg atacttaaag
     4381 tcatttggcg aaatgtatgt ttcttttact gttcgagggg atgtccagcg tagtagattc
     4441 agatagcttc cggccttatc gt
//
LOCUS       DAOSHR010000161         4441 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_17726_length_4441_cov_4.606247, whole genome shotgun sequence.
ACCESSION   DAOSHR010000161 DAOSHR010000000
VERSION     DAOSHR010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 4441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4441
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17726_length_4441_cov_4.606247"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(18..524)
                     /locus_tag="PLU28_08510"
     CDS             complement(18..524)
                     /locus_tag="PLU28_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35137.1"
                     /translation="MKKNIISLSFSFIFCLTTLLGLNYFSFPQDFPDNKFFIKIFDFL
                     KISSIPSQSITVAECSSNLEFYDITSLEYSYYGAVFIPEMNSIFTVPFSKLIKKEIFY
                     QTLAHEVMHFYLHNYFDLTHAEEEGFIIFYLEQKNLIPEYKEYYLTFMNVWEGNIHKF
                     LNSRRKKI"
     gene            complement(526..1272)
                     /locus_tag="PLU28_08515"
     CDS             complement(526..1272)
                     /locus_tag="PLU28_08515"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOG35138.1"
                     /translation="MIMKTFVVEDEPHSLSRLKTLLSEVQEISIIGQADNGIQAVSEI
                     NKLRPELVFMDINLPGKNGFEVISALEYRPKIIFITAYQEYAIKAFEENAVDYLLKPI
                     SLERLQSAVNKVIQSNKTLEDNVIDIIKKYMNSEKYLTRFSIKLEDEILILNQQDVFY
                     FKAEDKYVFLCTYDKEYYYDNTLKSLEEMLDPSVFLRIHKSYIVSISKIERLKKWFLS
                     EYSVELSDKAKSSLKVGRNFLPGLKDKLHF"
     gene            complement(1276..2331)
                     /locus_tag="PLU28_08520"
     CDS             complement(1276..2331)
                     /locus_tag="PLU28_08520"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOG35139.1"
                     /translation="MFKLKKFLLMLLLSELSGIAISFLIYLFSKTFDYFEINLTISLI
                     FSNSIGLIYYLISSIFKYYIADKLQNTLIKFFLKFFVLFASLFLSFEIAYFIIRYFFK
                     MAIFPDYFEIQNLLLVINIILFSVLIIFVIIYQKLRIEIEKKNRENENLRRLQTESKL
                     VALQSKINPHFLFNTLNSIIELAYESPKKVETVVLNLSTIYRKILYLADKQFFSVSEE
                     IELVKSYLEIEKIRMGIRLNYELNIDPQTLSIKIPPLIIEPIVENSVIHGIGPKPEGG
                     KISVTIIKKENNVIISVTDTGVGIKENNLKQGYGTSSVKERLGILYKNKFTFEIKNHS
                     MNDSGTDVTIIIPYKEA"
     gene            complement(2346..3026)
                     /locus_tag="PLU28_08525"
     CDS             complement(2346..3026)
                     /locus_tag="PLU28_08525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35140.1"
                     /translation="MKKLHMIILLLLGTIISFAVNPEDIYDLKAKIRDLEVTVSKNPK
                     DATALYQLALSYEVLSENGDEKYLENTRINFQNYLKLDPYNSVARVKYGKSLVMSGQK
                     NWFLPAKMWFIMSGTSEMTKAAEVDPENFNVRYIRGSTCFELTEYSFCIQTAITDFEY
                     ILYNKELFRPYFDSETINELNYKLGYCYIFTKNYEKAFLYLDDVINNHPYSIYYSPSI
                     ALRRKYTK"
     gene            complement(3050..3754)
                     /locus_tag="PLU28_08530"
     CDS             complement(3050..3754)
                     /locus_tag="PLU28_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35141.1"
                     /translation="MIEKWWVFPFIAILGISLLAFIPSPSNTLNPNKYIDSYENIFIK
                     ASTIDLYFDDTSDKIFLNDKLFVEEKKDAVYLTAPSQFSISSQSTKIVIGTKNRIQKL
                     RVDGISIKISGIAAITSIIIDGIGINIDSDISCESIDMKGTGITVKGLMDCTTAKING
                     TSVNLNIAMANNKDMDINCTSINATIKYFEDSPYFKDLRVSGLSGAMSLILPKNDFFK
                     VEKNISKNIAVNTFFY"
     gene            complement(3812..>4441)
                     /locus_tag="PLU28_08535"
     CDS             complement(3812..>4441)
                     /locus_tag="PLU28_08535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35142.1"
                     /translation="FTFIIILSLSFAALAANFGIGGPFGGFMPSDQMQNPLSGVKFEN
                     GLFYLGGMGFGKISDTFYMGGEGYDGSSSASDGRYMYKSSYGAFVVGTDINILSNLSL
                     NIGLGIGGMTDTIQALSAASGTSINNVQDGTARSYIKISRDYYTLAPQASLYFKFVDF
                     FGLNVTGRYMVGYSPFPWKLETNDNVSGIVDNSPLRFSYSIGAGLFFGF"
BASE COUNT         1482 a          728 c          720 g         1511 t
ORIGIN      
        1 aatagtttta aataaattca tatttttttc cttctactat taagaaattt gtgtatattc
       61 ccttcccaga cattcataaa agtcagataa tattctttat actcggggat aagattcttc
      121 tgttcaagat aaaaaattat aaagccttct tcttctgcgt gagttaaatc aaagtaatta
      181 tgaagataaa aatgcatgac ctcatgtgcc agagtctgat aaaagatctc ctttttaatg
      241 agcttagaaa aaggaacagt aaatatagaa ttcatctcag ggataaaaac tgctccgtag
      301 tacgaatatt caagagatgt gatatcataa aactccaggt tgcttgaaca ttctgccact
      361 gtaatacttt gtgatggtat agaggatatt ttcagaaaat caaatatctt tatgaaaaat
      421 ttattatccg gaaaatcctg cggaaaagaa aagtagttaa gtcccagaag agttgtcaag
      481 caaaatataa atgaaaaaga gagagagata atgttttttt tcattttaaa aatgtaattt
      541 atcctttagg ccaggtaaaa aatttcttcc gaccttcaga gatgattttg ctttatcgct
      601 tagttctaca gagtattcac tcagaaacca ctttttaagc cgttcaattt ttgaaattga
      661 aacaatgtag ctcttgtgta ttctgaggaa tactgacgga tcaagcattt cttcgagact
      721 tttgagagta ttgtcatagt agtattcttt atcgtaggta cataaaaaaa catatttatc
      781 ttcagcctta aaataaaaca catcctgttg attgagaata agtatttcat cttcaagctt
      841 tatggaaaat ctagtaagat acttttcaga gttcatatat ttttttatta tatcaataac
      901 gttatcttca agagttttat tggattgtat gactttattt actgcagatt gaagtctttc
      961 aagagagata ggcttcaaaa ggtagtccac cgcattttct tcgaaggctt ttattgcata
     1021 ctcctgatat gcagtaatga aaattatctt aggcctatat tcaagggcag atataacttc
     1081 aaagccattc ttgccaggaa ggtttatatc cataaaaacc aattccggtc taagcttgtt
     1141 tatttcagaa acagcttgga tgccgttatc cgcctgtccg ataatactta tttcctgtac
     1201 ttcagacagc aaagttttca gtcgcgaaag tgagtgaggc tcatcctcaa caacaaaagt
     1261 tttcattatc ataaatcagg cctccttata aggaattatt atagttacat cggttcctga
     1321 atcgttcata gaatggtttt ttatttcaaa agtaaactta tttttataga gtattccaag
     1381 ccgttctttt acacttgaag ttccgtaacc ttgttttaaa ttgttttcct taattccgac
     1441 accagtatca gttacagata ttatcacatt gttttctttt tttattattg taacactaat
     1501 ttttccgcct tctggttttg gtcctatacc gtggataaca gaattttcta ctataggctc
     1561 tattatcaac ggcggtatct taatgctgag agtctgcggg tctatattca gttcatagtt
     1621 cagtcttatt cccattcgta ttttttctat ttccagatag ctctttacca gttcaatctc
     1681 ttcacttact gaaaaaaact gtttatctgc aagataaagt attttcctgt atatagtaga
     1741 aagattaagt acaacagttt caactttttt tggtgattca taagcaagtt caattataga
     1801 attgagagta ttaaatagaa aatgagggtt gattttagat tggagagcaa caagctttga
     1861 ttcggtctga agtcttctga gattttcatt ttccctgttc tttttttcaa tctcaattcg
     1921 gagtttctgg taaataataa caaaaattat taaaactgaa aatagaataa tgtttattac
     1981 cagcagaaga ttttgaattt caaagtaatc tggaaaaata gccatcttaa aaaagtatcg
     2041 tataataaaa tatgcgattt caaaggatag aaataaagaa gcaaataaca caaaaaattt
     2101 cagaaagaat tttataagtg tattctgaag cttgtcggca atataatact taaaaataga
     2161 agatataaga tagtatatca agccaattga atttgaaaaa atcagggata ttgtaagatt
     2221 tatttcaaag tagtcaaaag ttttactgaa aagataaatc aaaaacgata tggcaattcc
     2281 ggataactca cttaatagaa gcattaaaag gaattttttt agtttaaaca tattttcacc
     2341 actgattatt tagtgtattt tcttcttaag gcgatactag gagaatagta aattgagtat
     2401 ggatggttat ttattacatc atcaaggtat aaaaaagctt tttcataatt tttggtaaat
     2461 atataacaat acccaagctt gtagttaagc tcgtttatag tttcactatc aaaatatggt
     2521 ctgaaaagtt ctttgttata aagtatatat tcaaagtccg taattgcggt ttgaatacaa
     2581 aaagagtatt ctgtaagttc aaagcacgtg gatcctctga tgtatctgac attgaaattt
     2641 tccggatcaa cctccgccgc tttagtcatt tcagaagtac cagacattat aaaccacatt
     2701 tttgctggaa gaaaccagtt tttctgacct gacataacaa gagattttcc atactttaca
     2761 cgagctacgg aattgtaagg atctaattta agatagtttt gaaagttaat ccgggtattt
     2821 tcaagatatt tttcgtcacc attttctgaa agaacctcat aacttaatgc cagctgatat
     2881 aaagctgtgg catctttggg attttttgag acagtgactt ccagatcacg tatttttgct
     2941 tttaaatcgt aaatatcctc agggttgaca gcaaatgaga taatagttcc caataacaac
     3001 aaaattatca tatgaagctt tttcatcggt accctccaac aagagcaaat caataaaaga
     3061 aagtgttaac agcaatgttt ttgcttatgt tcttttcaac tttaaaaaaa tcatttttgg
     3121 gaagtataag agacatagcc ccgcttaatc cgcttacacg taagtccttg aagtaaggag
     3181 aatcttcaaa gtacttgata gttgcattta tgcttgtgca gtttatatcc atatccttat
     3241 tattagccat cgcaatattc agattgacag aagttccgtt tatttttgct gttgtacagt
     3301 ccataagacc ctttactgtt attcctgtac ccttcatatc aatactttca caggaaatat
     3361 cagaatcaat gttaatgcct attccatcaa taataatact tgttatggcg gctatgcctg
     3421 atatttttat ggaaataccg tctactctga gcttttgtat tctatttttg gttcctatga
     3481 caatttttgt actctgggaa gaaatagaaa actgtgaagg tgcggtaaga taaactgcat
     3541 cttttttttc ttcgacaaaa agtttatcgt ttaaaaagat tttgtcagaa gtatcgtcaa
     3601 agtatagatc aatggtgctt gcttttataa aaatattttc ataagaatca atatacttat
     3661 ttggatttaa ggtatttgat ggagaaggta tgaaagccag aagagaaatg ccaagtattg
     3721 caataaaagg gaaaacccac catttttcta tcatgcgtac acctcgtaag gcaaaataga
     3781 cgggtcacaa gacccgtctc agtttttatt attaaaaccc aaaaaataat ccggcaccta
     3841 tactgtaaga aaatcttaat ggagagttgt ctactattcc agaaacgtta tcgtttgttt
     3901 caagtttcca tgggaatgga ctatacccta ccatgtatct tccggttaca tttagcccga
     3961 aaaagtcaac aaacttaaag taaagtgatg cttgaggagc caaggtatag tagtctctgc
     4021 tgatttttat gtaacttctg gctgttccat cttggacatt gtttatactg gttccggaag
     4081 ctgctgataa agcttgtata gtgtctgtca ttccgcctat gcccagtcca atattaagac
     4141 tcagatttga aagaatgttt atgtctgttc caacaacaaa agcgccataa gaacttttat
     4201 acatataacg tccatcggaa gctgaagaag aaccgtcata cccttctcca cccatataaa
     4261 aggtatctga aatttttcca aaacccatac ctccgagata aaaaagaccg ttttcaaact
     4321 taacaccaga aagggggttt tgcatctggt cactaggcat aaaaccacca aaaggtccgc
     4381 ctattccaaa atttgcggct aatgctgcaa agcttaaaga taaaatgata ataaaagtaa
     4441 a
//
LOCUS       DAOSHR010000162         4438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_17746_length_4438_cov_4.731462, whole genome shotgun sequence.
ACCESSION   DAOSHR010000162 DAOSHR010000000
VERSION     DAOSHR010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 4438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4438
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17746_length_4438_cov_4.731462"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..695)
                     /locus_tag="PLU28_08540"
     CDS             complement(<1..695)
                     /locus_tag="PLU28_08540"
                     /inference="COORDINATES: protein motif:HMM:NF019880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35143.1"
                     /translation="MTVDINKYFVEYYRFLTGDTLEEKKRIKCINPMHQDKRPSMVFY
                     TTRTPHLYCFGCQRLFNIYDIGSILAGKSPEEVETDIYTHIDPSADPQKIKDLSSWML
                     MILNEITDMASQELNTKLSLEGRSYLAKKGINQELCMKYSIGHINKADAFINRLKQKI
                     SDDTYAQIDKHFYIKNNLKNNSIIFPLKEEGKTIGLCTRLLDDKTQKYISSKNSILFQ
                     KKKFLYNNQVLKN"
     gene            complement(703..966)
                     /gene="cas2"
                     /locus_tag="PLU28_08545"
     CDS             complement(703..966)
                     /gene="cas2"
                     /locus_tag="PLU28_08545"
                     /inference="COORDINATES: protein motif:HMM:TIGR01573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HOG35144.1"
                     /translation="MFIVMTYDIGEKRVSKVHKITKRYLNWVQRSVFEGEITQSKYNE
                     LRNKILNVINKKEDSVNYYKTKSKAYVSITNDGTEKNHYESMI"
     gene            complement(1018..2352)
                     /locus_tag="PLU28_08550"
     CDS             complement(1018..2352)
                     /locus_tag="PLU28_08550"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOG35145.1"
                     /translation="MDKSLLVVPCFDNPYICATEKEKNVVLSIKVQPEEGLEGGTTTS
                     TGIDFCIVLDTSGSMTERISDDKTSERKRDLAIKATKNLYDFIRPNDTVSMIFYDSFS
                     RTILDHEKNISRAIFDEKVEESRCYSGGTNITNALMAATDLMTKKLSPNMKKVIFLTD
                     GKTVDDTEADAIKAAILLAEKKISITTLGIGKDFNFNFILELVKPCKGTSDSIEKEEE
                     AIQVFEKTLKRSQNIVLNDVELELNVSNEVRVNEYFRSVPQTTYMGKVPLKSDRKFVI
                     RLDDIEKNRYYEYLFEITIPTPNKEYEGKFRVAKSVLKYTVVSTNQPVEKSCDILIDI
                     TKDEELAEKEYSTIKTTYKRCIIQKLDNQLNDFLANKDHSNVIRILKELISKTQEIDE
                     NERSDYYKSMYEEYITTNQISQQKLNSATKSSTIINGDGLINENQQEPPDIF"
     gene            complement(2385..3935)
                     /locus_tag="PLU28_08555"
     CDS             complement(2385..3935)
                     /locus_tag="PLU28_08555"
                     /inference="COORDINATES: protein motif:HMM:NF025053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HOG35146.1"
                     /translation="MSFINRREDTVLMYTYENINWSFEYTIFKVEPHINGLNYPIDTK
                     KPNEAKFVVSKGYKSIYDLHKQSIEDKLQFVHKLYSILSEIESQGYKVYNFDLESVVY
                     DEKGDPCILLFYPVYSIKDNKEIVNNLDGFIKVDPNKATNSFILAEIFGEILFPKEYK
                     KISDKKMKIKFFKEKMCEVPYSKYLRDFYNWHKKAVSNKYSLTDSYEEFKKAYKKMKE
                     RNEKKTQKLSLIKRTGRTSEGIGKLKESDETKSEEEVNQDAFYMDVDQKEKIAITVVM
                     DGVSTASVGNGNMASSILKETVTKLWGNLKKGDINDSTVEKFVRKIIYEASGKIIEYA
                     NEYKTENTKANDIMASTIAGVIINGSKAYIFSAGDSKIYIKGKDYFFPLNPEHNKQTE
                     ELLKGKEVQNESSPITECVGKALLKNNNFVFNPVKCYISAFDILADEDIIVCSDGVFD
                     FWKGFDWSEKEDNFFSDYTKMEEQFKQVKKISSRILSTLDQNMAGDNITITILRPVFE
                     TEQRSNVD"
     gene            complement(3953..>4438)
                     /locus_tag="PLU28_08560"
     CDS             complement(3953..>4438)
                     /locus_tag="PLU28_08560"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOG35147.1"
                     /translation="YEEEFCECGGTYTVIDDGMQEPLLKEDNNTTVVVEAPEPSENSE
                     RPEEYLKDFGFMPMIEVYKEKNKLFTFNIEYDEITIGRKTSRSFPEIDLSIYDQNKYL
                     SRNHLAVLRLNDKYYARLLSSQNISYLNNSVMKYDEDYELKDGDKIILTKELGIIVRN
                     I"
BASE COUNT         1110 a          816 c          724 g         1788 t
ORIGIN      
        1 atgtttttca aaacttgatt attgtataaa aatttctttt tctgaaaaag aattgaattt
       61 ttggaagaga tgtatttttg agttttatcg tccaacagtc ttgtgcaaag acctatggtt
      121 tttccttctt ccttcagagg aaagataatg ctgttgtttt taagattgtt ttttatgtag
      181 aagtgtttat ctatctgtgc atatgtatca tctgatattt tttgttttaa tctgtttatg
      241 aacgcatctg ctttgttgat gtgacctatg ctatatttca tgcacagttc ctggtttata
      301 ccttttttgg caaggtagct tcgcccttca agagatagtt tggtgttgag ctcttggctg
      361 gccatatctg ttatttcgtt aagtatcata agcatccagg atgatagatc tttaattttc
      421 tgaggatctg cagatggatc aatatgtgta taaatgtcgg tttcaacttc ttccggactt
      481 ttccctgcaa gaatactgcc tatatcatag atattaaaaa gtctttggca tccaaaacag
      541 tataggtgag gtgttctggt ggtatagaaa accattgaag gtcttttgtc ctggtgcatc
      601 gggttaatgc actttattct ctttttttct tcaagcgtgt ctccggttaa aaaacggtag
      661 tactccacaa agtacttatt tatgtctact gtcatttttc acctaaatca tgctttcata
      721 atggtttttt tctgttccat cgtttgtaat ggatacgtat gctttggact tggttttgta
      781 gtagttaacc gagtcttctt ttttgtttat tacgtttagt attttatttc tcagttcgtt
      841 gtacttggac tgggtgatct caccttcaaa tactgaccgt tgaacccagt taagatatct
      901 tttggttatt ttatgaacct tggatactct tttttctccg atatcgtacg tcataacaat
      961 aaacattcat tgcctcctga aaaagaagcc gtgctttggg cacggctaga ggtttgttta
     1021 aaatatatcg ggtggttcct gctgattttc gtttataagc ccgtctccgt ttattattgt
     1081 tgagcttttg gttgcactgt taagcttttg ctgggagatt tgattggttg ttatatactc
     1141 ttcgtacata ctcttgtagt agtctgatct ttcattttcg tctatttcct gggtctttga
     1201 tataagctct ttcaaaatcc ttattacatt actgtggtct ttatttgcca ggaagtcgtt
     1261 aagctggtta tccagctttt gtattatgca tcttttatac gtggttttta tggtactgta
     1321 ctctttttct gcaagttcct cgtctttggt tatatctatg agtatgtcgc agcttttttc
     1381 aacgggttgg tttgtcgaaa caactgtgta tttgagaact gactttgcca ctctgaactt
     1441 tccttcatac tctttgttag gagtgggaat agttatttca aagaggtact cgtaatatct
     1501 gtttttctcg atatcgtcaa gtcttattac aaactttctg tctgatttaa gtggaacctt
     1561 ccccatgtac gttgtctgag gcacagaacg gaagtactca ttgactctta cttcgtttga
     1621 aacattaagt tcaagctcaa catcatttaa aacaatgttc tgacttctct ttaatgtttt
     1681 ttcaaatacc tggatggctt cttcttcctt ttctattgaa tcgcttgttc ctttgcaagg
     1741 ttttacaagc tccagaataa agttaaagtt gaagtctttt cctataccaa gcgttgttat
     1801 tgaaattttt ttctctgcaa gcaatattgc agcttttatt gcatcagcct cggtatcgtc
     1861 aactgttttt ccgtctgtga gaaatattac ctttttcatg ttagggctta atttcttcgt
     1921 catcagatct gtggctgcca tgagtgcatt tgttatgttt gtgcctccgc tgtaacaacg
     1981 agattcttcc actttttcat caaatattgc tctgctgata tttttttcat gatccagtat
     2041 tgtccgtgaa aaagagtcgt agaaaatcat agatactgta tcgttaggtc ttatgaagtc
     2101 atataagttt ttggttgctt taatagcaag atcccttttt cgttcagatg tcttgtcatc
     2161 tgatattctt tctgtcatag aaccgcttgt atcaagtaca atacaaaaat ctatcccggt
     2221 agatgttgtt gtgcctccct ccaacccttc ttcaggctga acctttatag ataaaaccac
     2281 atttttctct ttttctgtag cacatatgta cggattatca aagcacggaa caaccaataa
     2341 tgatttatcc aatttttatc cctcctgtgt gtatgttcaa gttattaatc gacatttgac
     2401 ctctgctctg tttcaaaaac gggtctgagt atggtaattg tgatattatc gcctgccata
     2461 ttttgatcaa gagtactgag aattcttgag gaaatttttt ttacctgttt gaactgctct
     2521 tccatttttg tatagtcact gaaaaagttg tcttcttttt cagaccagtc aaaacctttc
     2581 caaaaatcaa agaccccgtc tgagcaaact attatatctt catcagcaag aatatcaaat
     2641 gctgagatat agcattttac agggttaaaa acaaagttat tgttttttag caaggctttc
     2701 ccaacacact ctgttatggg ggagctttca ttttgaactt ctttgccttt taaaagctct
     2761 tctgtttgtt tgttatgctc gggattcagc gggaaaaaat aatctttccc ttttatgtat
     2821 atctttgaat ctcctgctga aaatatatac gctttactgc cattaattat caccccggca
     2881 attgtgcttg ccattatatc gtttgcttta gtgttttctg tcttatactc gtttgcatat
     2941 tctattattt tccctgaggc ttcatatata atttttctga caaatttttc aactgtactg
     3001 tcgttaatat ctcctttctt gagattgccc cagagttttg tcactgtttc tttaagaatg
     3061 ctgctggcca tattgccatt tcctacggaa gctgtgctta caccgtccat aacaactgtt
     3121 atagcaattt tttctttttg atctacatcc atataaaatg catcttggtt tacttcctct
     3181 tcacttttgg tttcatccga ttctttcagt tttcctattc cctctgaagt tctaccggtt
     3241 ctcttgatca gacttaattt ttgcgttttt ttttcatttc tttctttcat ttttttgtat
     3301 gcttttttaa actcttcgta agagtctgta agagagtatt tgttggaaac agcctttttg
     3361 tgccagttat aaaaatctct cagatatttg ctgtaaggta cttcacacat tttttcttta
     3421 aagaatttta ttttcatttt tttatcgctt atctttttat actctttagg aaaaagtatt
     3481 tctccaaata tttctgcaag aataaaactg tttgtggctt tatttggatc aactttaata
     3541 aaaccatcca gattgtttac tatctctttg ttgtctttta tagaataaac aggataaaaa
     3601 agcagtatgc aaggatcgcc tttttcatca tatactacgc tttccaagtc aaaattatat
     3661 actttgtatc cttgactttc tatttctgac agtatcgaat aaagtttatg cacaaactga
     3721 agtttatcct ctatgctctg cttatgaaga tcataaatac ttttatatcc ttttgaaact
     3781 acgaactttg cttcattagg tttttttgta tcaatagggt aatttaaacc gtttatgtgc
     3841 ggttctactt taaatattgt atattcaaag ctccagttaa tattttcgta tgtgtacatt
     3901 agtacggtat cttccctacg gtttataaaa ctcaagctta taccctcctt ttttagatgt
     3961 tcctgactat tatccccagt tctttggtga gaatgatctt atctccgtct tttaattcgt
     4021 agtcttcatc atacttcata acagagttat ttaaatagga aatattttga gatgacaata
     4081 accttgcgta gtatttatcg tttaatctta aaacagcaag gtgattgcgg cttaagtatt
     4141 tattctgatc atatatagaa agatcaatct cgggaaatga tctggaagtc tttctgccta
     4201 tcgttatctc gtcgtactca atattaaatg taaataattt gtttttttcc ttgtacacct
     4261 ctatcatagg cataaaacca aagtccttta aatattcttc aggcctctca gagttttcac
     4321 tcggttcagg agcttcaacc acaacagtcg tattgttatc ttcttttagg agcggttctt
     4381 gcataccatc atcaataaca gtataagttc ctccgcattc gcagaactct tcttcata
//
LOCUS       DAOSHR010000163         4433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_17772_length_4433_cov_5.738693, whole genome shotgun sequence.
ACCESSION   DAOSHR010000163 DAOSHR010000000
VERSION     DAOSHR010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17772_length_4433_cov_5.738693"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(96..1328)
                     /locus_tag="PLU28_08565"
     CDS             complement(96..1328)
                     /locus_tag="PLU28_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOG35148.1"
                     /translation="MNIESKLSTTSKNIKSSIIRELLKISDPAMISFGGGVPDPETFP
                     REELAAIASEVVSKEYKFTLQYGTTEGDDVLKKQYIRLLEEHENIKGLNNDNLLITVG
                     SQQALYLAGLTFLDEESYCGVTRPVYLAAASAFAQRNPKFISVNLSEKGIDTEYLESE
                     LIKLRERNELHKFKFMYVVTNFHNPAGITMPIENRKKLIELAEKYDFIILEDDPYGAL
                     RFEGTKVPSIFSMNSERVIMLNTFSKILSPGMRIGVIIGNTQAIRRMAMAKQAVDLCC
                     SPVTQRLTARFIERYDLFEQIKPTIELYKHKKDVFMKAMQDNLGDIKGASWTRAEGGL
                     FTWLTLPQQIDTMEMFEMAKKEKVLYIPGEAFFVDNPLKNTMRLSFCLPTEDQLVEGM
                     KRLRRTIDKYCAAKGIKL"
     gene            1700..2974
                     /gene="glyA"
                     /locus_tag="PLU28_08570"
     CDS             1700..2974
                     /gene="glyA"
                     /locus_tag="PLU28_08570"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOG35149.1"
                     /translation="MWENVKKTDSEIFGVIEKELKRQEYGLELIASENFASKAVMEAM
                     GSILTNKYAEGYPKKRYYGGCIFVDEIETIARERAKKLFNVKYANVQPHSGSQANMGA
                     YFSVINPGDTVMGMSLSNGGHLTHGAPVNFSGKLYNFVSYGVSIETEMIDYNEVEKLA
                     KEHKPKLIVAGGSAYGRIIDFKKFREIADMVGAKFMVDMAHFAGLVAAGIYPNPCEYA
                     DIVTSTTHKTLRGPRGGIILTNSEDLYKEINKAIFPGIQGGPLMHVIAAKAVAFNEAL
                     SPEFVQYQKNVVENAKVLSSELAKNDLTIVSGGTDSHLMLVDLNKINITGKALEIALG
                     KCDITVNKNTVPKETRSPFVTSGIRVGTPAVTTRGMNKDDMTVIAKLIATVAKNVKDE
                     EGNIESSVIEEVSSKVHSLCSKYPLYKDLIER"
     gene            3049..3678
                     /gene="upp"
                     /locus_tag="PLU28_08575"
     CDS             3049..3678
                     /gene="upp"
                     /locus_tag="PLU28_08575"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HOG35150.1"
                     /translation="MNNFHVVEHPLIKHKLSIMRNKETGPKEFRELLNEITLLLTYEA
                     LRKLPTVEVDVQTPITQIKGEMVEDKKVTVIPILRAGLGMLDGVLNLVPNASVGFIGI
                     FREPTTLNPVEYYAKFPNFTESHQIFILDPMLATGHSMKHAIDVVKKNGGVNITVMSL
                     ITAPEGLKIVQQAHPEVDIYTASLDEKLNDHAYIVPGLGDAGDRLFRTK"
     gene            3686..>4433
                     /locus_tag="PLU28_08580"
     CDS             3686..>4433
                     /locus_tag="PLU28_08580"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HOG35151.1"
                     /translation="MSELLKHSIIFACATFISRILGLVRDGFFAHYFGTAPEYSVYLL
                     AIGLPFYLRQMFAEGALSSAFIPLFTRKEGKEKQVFLSTAFWLLLIVTIILYDIVLIF
                     SGQISFVLGSGLTPEMRDLMSFLLKITFPFITFISLWAIISGVLNTYNMFFVTAISPA
                     LSNIVIILAVVMSGFFIPKILGPTIGFVLGGVLQLVFVLFFLRRTNMRITLDYNRTYT
                     KEILNLFGPAFLGASVSMLNTVIDTSIATWT"
BASE COUNT         1318 a          846 c          831 g         1438 t
ORIGIN      
        1 ttcccggctg agaatatcat cagtaaacaa agaaaccttg gttgatgtgg aaccgggatt
       61 tataaccaat atttttttca tgtgaactcc tcctgttaca gttttattcc cttagcagca
      121 cagtacttgt ctatagtcct tctcaacctt ttcatgcctt ctacaagctg atcctcggtt
      181 gggagacaga aagaaagcct catagtattt ttaagaggat tatccacaaa gaaagcttcg
      241 ccagggatat aaaggacttt ttcctttttt gccatttcaa acatttccat agtatctatc
      301 tgctgtggaa gcgtaagcca tgtaaaaaga ccaccttcgg ctcttgtcca gcttgcacct
      361 tttatatctc cgagattatc ctgcatagcc ttcataaata cgtccttttt atgtttataa
      421 agttcaattg tgggttttat ctgttcaaag agatcataac gttcaataaa ccttgctgta
      481 agcctttggg taactggaga acagcataga tcgacagcct gctttgccat ggccattctt
      541 ctgattgcct gagtatttcc tattattacg cctattctca tgccaggtga aagaatcttt
      601 gagaatgtat tgagcattat tactctttcg ctgttcattg agaagatact cggtactttg
      661 gtgccttcaa atctcaatgc tccgtaagga tcgtcttcaa gtattataaa atcgtacttt
      721 tcagcaagct caataagctt tttcctgttt tcaataggca ttgttattcc ggcagggtta
      781 tggaagttgg ttactacata catgaatttg aacttgtgca gctcgtttct ttccctgagt
      841 tttataagtt ctgattccag atactccgta tctatacctt tttcactcaa atttacgctt
      901 ataaactttg gatttctctg tgcaaaagca cttgctgctg caagataaac cggtcttgta
      961 acaccgcaat aactctcctc atcaagaaat gtaagtccgg caagataaag agcctgctgt
     1021 gaaccaactg ttattaaaag attgtcatta tttaagcctt ttatattttc atgttcttca
     1081 agaagcctta tatactgttt tttcagtaca tcgtcccctt cggttgtacc atactggaga
     1141 gtaaatttat actcttttga tacaacttcg ctggctattg cagctagttc ttcacgcgga
     1201 aaagtttccg gatccggtac tccccctccg aaagatatca tggcgggatc gcttatcttc
     1261 aacaactctc ttatgatcga agatttaatg ttcttggatg ttgttgagag cttactctct
     1321 atgttcatac ttacacctcc gttatctatt aaaatctttt actttacatc tattaaaatc
     1381 ttttattttt gggtcttatc tgctttcatt atcttatata attcaattcc ctactatatt
     1441 ttattatcaa tacctatatt ttccaaaatg atttttagat gtatatgctt gattatcctc
     1501 aaataacttc caatatctgc gattataaac aatatgaaaa aaatttcatg aaatctattt
     1561 catacagttt ctactcttct ttatacaagc agtttacaaa aaaatgtcct tttaccttta
     1621 tgatttgaaa taattgtctg ctttagtata taatataagt aagtgtcgtt aaaaaagcaa
     1681 acagacagga ggactcaaca tgtgggaaaa cgttaaaaaa accgacagtg aaattttcgg
     1741 agtgatcgaa aaggagctga aaaggcagga atacggtctt gagctgatag cttcggaaaa
     1801 ttttgcctct aaagctgtaa tggaagctat gggcagtatt cttactaaca aatatgctga
     1861 aggatatccc aagaaaagat attacggcgg atgtatattt gttgatgaga tagaaacaat
     1921 tgcacgcgaa agagcgaaaa aactttttaa tgtaaaatac gccaatgtcc agcctcattc
     1981 cggttcacag gcaaatatgg gagcctattt ttcagtaatc aacccaggtg ataccgtaat
     2041 gggaatgtct cttagcaacg gcggacatct tactcacggc gccccggtaa acttttcagg
     2101 taaattatat aatttcgttt cctatggtgt ttcaattgaa acagagatga ttgattacaa
     2161 tgaagtagaa aaattggcta aagaacataa gccaaagcta attgtagcag gcggcagtgc
     2221 ttacggaaga attatcgatt ttaaaaagtt cagggaaatt gccgacatgg taggagccaa
     2281 gtttatggta gacatggctc attttgccgg tcttgtcgca gctggaatat atccaaatcc
     2341 ttgcgaatat gctgatattg tcacatctac cacccacaag actctgaggg ggccaagagg
     2401 cggtataatt cttaccaaca gcgaggatct ctataaagag attaacaaag ccatattccc
     2461 aggcattcag ggcggtccgc tcatgcatgt aatagcagca aaagccgttg cgtttaatga
     2521 agctctttca ccagaatttg tccagtacca gaaaaatgtt gtcgaaaatg caaaggttct
     2581 ttcatcagag cttgccaaga acgacttgac catagtatcg ggcggtacag actcacatct
     2641 tatgcttgta gaccttaaca agattaatat taccggtaag gcacttgaaa tagctttggg
     2701 aaaatgcgat atcactgtca ataagaatac tgtccccaaa gaaaccagat caccgttcgt
     2761 aacaagcggt ataagagtag gaacacctgc tgttactacc agaggaatga ataaagacga
     2821 tatgacagta attgcaaagc ttatagcaac agtagcaaaa aatgtaaaag atgaagaagg
     2881 caatattgaa agctcggtta ttgaagaggt ttcttcaaag gttcattctt tgtgttctaa
     2941 atatcctctt tataaagatt taattgaaag ataatattta atcccccggt ttatggggga
     3001 ttttttttga aaatgtgata aaatacttac agtctggagg tgcatataat gaataatttt
     3061 cacgttgttg aacatccttt gataaagcat aagctttcca taatgagaaa caaggaaaca
     3121 ggtccaaagg agttcagaga acttcttaac gaaatcactc ttctgcttac ttatgaggca
     3181 ctgagaaagc ttcctacagt tgaggtcgat gtacagactc ctataacgca gattaagggt
     3241 gagatggttg aagacaaaaa ggttaccgtg atacctatat tgcgagcagg tcttggtatg
     3301 cttgatggag tacttaatct ggttcctaat gcaagcgttg gttttatagg aatattcagg
     3361 gaacctacca ctcttaatcc tgtggagtac tatgcaaagt tcccgaattt tacagaatca
     3421 catcagatat ttattcttga tcccatgctt gcgacaggtc attccatgaa acatgccata
     3481 gatgttgtca aaaaaaatgg tggtgtaaac ataacagtca tgtctctcat aacggcccct
     3541 gaaggtctga aaatagttca gcaggctcat cctgaggttg atatatatac agcatcgctc
     3601 gatgaaaaat taaacgacca tgcctatata gttcctggtt tgggtgacgc aggcgacaga
     3661 cttttcagaa caaaatagac cttatatgtc tgaactgctt aaacattcaa taatatttgc
     3721 ttgtgccact tttataagca ggatactcgg acttgtgcgc gatggctttt ttgcccatta
     3781 cttcggcact gcaccggagt attcggttta tcttttagcc attggtcttc cattttatct
     3841 tagacaaatg tttgctgagg gagctctttc ctcagcattt attcctttat tcacaagaaa
     3901 agagggtaaa gaaaagcaag tgtttttaag tactgcattc tggctcttac ttatagttac
     3961 aataattctt tacgatatag tactgatctt ttccggtcag atctcttttg ttcttggttc
     4021 aggattaact ccagaaatga gggatctgat gagttttctt ctgaagatta cttttccgtt
     4081 tataactttt atttccctat gggcaataat ttcaggtgtt ctgaacactt acaacatgtt
     4141 tttcgttact gccatatctc cggcattaag caatattgtt attatattag ccgttgtaat
     4201 gtcaggtttt tttattccga agattttggg tcctactatc ggttttgttc tcggaggagt
     4261 tcttcagctt gtttttgtcc tatttttcct tagaaggaca aacatgcgca taactcttga
     4321 ttataacaga acctatacaa aagaaatctt aaaccttttc gggccggcat ttttgggagc
     4381 atctgtttct atgctcaata cggtaataga taccagcatt gctacatgga cag
//
LOCUS       DAOSHR010000164         4404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_17915_length_4404_cov_4.568866, whole genome shotgun sequence.
ACCESSION   DAOSHR010000164 DAOSHR010000000
VERSION     DAOSHR010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 4404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4404
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17915_length_4404_cov_4.568866"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..986)
                     /locus_tag="PLU28_08585"
     CDS             complement(<1..986)
                     /locus_tag="PLU28_08585"
                     /inference="COORDINATES: protein motif:HMM:NF037626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOG35152.1"
                     /translation="MLKEINHELVNIIKEKSLENEGSTVFVLPEYSNFDINIENAEIF
                     PDFDIFPFEEMDISSKVKSLRLRTLFSAATSSRVTVITSLHALTRFSIPKESFYIKKY
                     RIGDSFEENLYDIGYQSSFRSAVSAVRSHGEYARRGFIRDIFIPIYDNPLRLELFDNQ
                     IERISFFDPNTQKSVSEIEEFIMVAGSESFKSPENISLFEKRAGMALSMSGEKEAITV
                     DMINALPGILFKDKKTILDYLDKQKLEIYIVNKEKVMSSFAEKERENLEMCSFSAARD
                     IYRRYGGSGLEVLSLFKSIEIDVDVPTKYYTKELDAQQDITTIPLIDWEDISS"
     gene            complement(964..1695)
                     /locus_tag="PLU28_08590"
     CDS             complement(964..1695)
                     /locus_tag="PLU28_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35153.1"
                     /translation="MLFRRQLTVKEIDLEVYSMLNTLKFDNISFADIRDVSPFFVFID
                     RSEELDIDLIDAGFEDGENAVVIAQPDIFPSEVNEGCNFNDTTLKDAVLDVQTKVMNE
                     ENISCPKILAQDEPKVMIKSFSDIRTTIKEKKVKCIVVKNPVKELKISIEKKQILQMV
                     EIIVKEHNVVISDLAFSGFYMQVPVARDVRYSWLGKKLCCESDSVPKVYMNAAGFYNS
                     RRNERYLKFIRDPATEEKSVKRNQS"
     gene            complement(1674..2174)
                     /locus_tag="PLU28_08595"
     CDS             complement(1674..2174)
                     /locus_tag="PLU28_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35154.1"
                     /translation="MLGYFSVYKSGDEKYSGGVLIINEKGMPVSFKYTSPIKPTKIQK
                     IIYGNNLKSYIASEIIGKKISEIAKDAEVLLVQENETLDFLSTDKPVAQLVSSKALSE
                     SSSFNGKEGIITLGQNYSAKISFSSEVSQELFKKISDLAEIFDILEPFGRLNEALSFV
                     CSSEDS"
     gene            complement(2191..2499)
                     /locus_tag="PLU28_08600"
     CDS             complement(2191..2499)
                     /locus_tag="PLU28_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35155.1"
                     /translation="MPPIHDLQVVVVKGVDVSNSIQAVNSASNSGRMVELVKQVQEFE
                     NRMKKVKDNTKEEKVNKDGHKGSMPANSSVEAKKGPEKASSEKSDFDDYRGKILDIRY
                     "
     gene            complement(2502..3200)
                     /locus_tag="PLU28_08605"
     CDS             complement(2502..3200)
                     /locus_tag="PLU28_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HOG35156.1"
                     /translation="MKYSLNKDQIIKNFLPKIKIIALNLLSTLPKSVELDDLIQEGLI
                     GLLQAYDRYDPSMGANFYTYGMNRIRGAMLDYLRRIDWLPKELRHLIKGYENFMADNP
                     DKHFTDEEIMDNLKISHDDFEKLKFSVRKSQILELDAYLTDKSYEEGMERAIADDADD
                     PEVNAYKEILNEKLKEAVEELSEKDQLILSLYYEKGLTFREIGEVIGVSESRISQIHT
                     AIIARLKRSLKGDE"
     gene            complement(3224..3709)
                     /gene="cheD"
                     /locus_tag="PLU28_08610"
     CDS             complement(3224..3709)
                     /gene="cheD"
                     /locus_tag="PLU28_08610"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemoreceptor glutamine deamidase/glutamate
                     methylesterase CheD"
                     /protein_id="HOG35157.1"
                     /translation="MNKKIIGIGEYALDNNPIVIVTLGLGSCVGVCVRDKTKNIGAMV
                     HVMLPDSSGNIGPKPGKYADSGIDLIYSELLKIGASASNLEAKIAGGAAMFGTSSQTM
                     DIGKRNIEAVKLKLKQKGVRIIAEDVGGNRARSIEFNIATGELMVKKVGGGEKVELYN
                     I"
     gene            complement(3702..4334)
                     /gene="cheC"
                     /locus_tag="PLU28_08615"
     CDS             complement(3702..4334)
                     /gene="cheC"
                     /locus_tag="PLU28_08615"
                     /inference="COORDINATES: protein motif:HMM:NF041093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CheY-P phosphatase CheC"
                     /protein_id="HOG35158.1"
                     /translation="MSLYDDIDNTRLDALKELGNIGTGNAATSISIMLDRKVEISVPD
                     VKIVPIEHIWQRFKDPEEIVAGANIGIGGELNGSILFLMGTDSIKKMLELMMLPKPDD
                     LTQLDEMTRSAIGELGNIMCSSYIVSLSTFTGLNIFSMPPGVVVDMLTAIVSEVSLMN
                     TEGEDFIIFIETSMNVGEDIGDISGYIIYIPDNESLKKILKKIGLGIDNE"
BASE COUNT         1244 a          984 c          647 g         1529 t
ORIGIN      
        1 gatgagatat cttcccagtc tataagcgga atagttgtta tatcctgctg agcatccaac
       61 tcttttgtat aatacttagt tggaacatca acatctattt cgatactttt aaataaagaa
      121 agaacctcca aaccacttcc gccataacgc ctatatatat cccttgcagc agaaaaactg
      181 cacatttcaa gattttcacg ttctttttca gcaaaactac tcattacttt ttctttgttt
      241 actatgtaga tctctagctt ttgcttatca agatagtcaa gaatagtttt tttatcctta
      301 aaaaggattc cgggcaaagc gtttatcata tcaaccgtta ttgcttcctt ttctccgctc
      361 atggagagtg ccattccggc tcttttttca aacaaactta tgttttccgg acttttaaac
      421 gattcgcttc ccgcaaccat tataaattct tcaatctcag aaacagattt ctgggtgttc
      481 ggatcaaaaa agctgatcct ttcaatctga ttgtcaaaaa gttcaagtcg taaagggtta
      541 tcgtatatcg gtatgaatat atcccttata aagcctcttc tggcatactc tccgtggctg
      601 cgaacagcac tcacagcact tctgaatgat gactggtatc cgatatcgta aagattttct
      661 tcgaaagaat cccctattct atattttttg atataaaaac tctctttggg tattgaaaat
      721 cttgttaatg catgtaaaga agtaatcaca gtaaccctgg acgaggttgc tgcagaaaaa
      781 agagttctga gtcttaggct cttgactttt gaagatatat ccatttcttc aaaagggaat
      841 atatcaaaat ccggaaatat ctctgcattt tctatgttaa tatcgaagtt tgaatactca
      901 gggagcacaa aaacagttga gccctcattt tccaggctct tttctttaat tatattgaca
      961 agctcatgat tgatttcttt taacactttt ttcctccgtt gccggatccc ttataaactt
     1021 aagatatctc tcatttcttc tggaattata aaaaccggct gcattcatat aaaccttagg
     1081 cacactgtca gactcgcagc aaagcttctt tcctaaccaa gaatatctga catctcttgc
     1141 caccggaacc tgcatgtaaa accccgaaaa tgcaagatct gatattacta cattgtgctc
     1201 cttaactata atctctacca tctgcagtat ctgttttttt tctatagaaa ttttcaattc
     1261 cttcacagga ttttttacta caatacattt tactttcttt tctttaatag ttgtgcgtat
     1321 gtcagaaaat gattttatca taacttttgg ttcatcctga gcaagtattt taggacagct
     1381 tatgttttct tcattcatta ccttggtttg aacatcaaga acagcatctt ttaacgtagt
     1441 atcgttaaaa ttacagcctt catttacctc tgaaggaaaa atatcaggtt gtgcaataac
     1501 aactgcattc tctccatctt caaatcctgc atcaataaga tcaatatcta attcttcaga
     1561 cctgtcgata aaaacaaaaa acggactaac atctctaatg tccgcaaagc ttatattatc
     1621 aaactttagt gtgttgagca tggaatatac ttccagatca atttctttta ccgttaactg
     1681 tcttctgaag agcatacgaa tgaaagtgcc tcatttaacc ttccaaaagg ctccagtata
     1741 tcaaatattt ctgcaagatc ggatatcttt ttaaacaact cctggcttac ttcagaggaa
     1801 aaagatattt tagcagagta attctgtcct aatgtaataa tgccttcttt gccgttaaaa
     1861 ctgctgcttt ccgataaagc cttgctgctt acaagctgag ctacaggttt atcagtgctt
     1921 aaaaaatcca gagtctcatt ttcctgaaca agtaatactt cagcatcttt tgcaatctcg
     1981 ctgattttct tacctattat ttccgaggct atatagcttt ttaaattatt accgtatatg
     2041 attttttgta ttttagttgg ttttattgga gaagtatact taaaagagac aggcattcct
     2101 ttttcattta tgatgagtac cccgcccgaa tatttttcat ccccggactt ataaacactg
     2161 aaatatccca acatcggtta ccctcctcat tcagtatctt atatccagta ttttgcctct
     2221 gtaatcatca aaatctgatt tttcactgct agctttttct ggtccttttt ttgcctcaac
     2281 agaagaattg gcaggcatac tgcccttatg gccatccttg tttacttttt cttctttagt
     2341 gttatcttta acttttttca ttctgttctc aaactcctga acttgtttca caagctctac
     2401 catacgtccg gaattagaag ccgagtttac agcctgaata ctgtttgata cgtcaacacc
     2461 tttgaccaca acaacctgaa gatcgtggat aggaggcata tttattcatc tcccttcaaa
     2521 gatctcttca gccgtgctat tatcgccgta tgtatctgag aaattctaga ttcgcttact
     2581 cctataactt cacctatttc cctgaaagtg aggcctttct cataataaag cgacagtatc
     2641 agctgatcct tttcgctaag ctcctcaaca gcttctttaa gcttttcatt aagaatctct
     2701 ttgtaggcat ttacctcagg atcgtcagca tcatcagcta ttgctctttc cattccttct
     2761 tcataacttt tatccgtaag gtaggcatca agttcaagta tctgactttt cctcacagaa
     2821 aattttagtt tttcaaaatc atcgtgactt attttaagat tatccattat ctcttcatct
     2881 gtaaaatgct tatcggggtt gtctgccatg aaattctcgt atccctttat aagatgcctc
     2941 agttcttttg gcaaccaatc aattctcctc agataatcaa gcattgcacc acgtattcta
     3001 ttcatgccat aagtatagaa attagccccc attgaaggat catacctgtc ataagcctga
     3061 agaagaccta taagtccctc ctgtatcaga tcatcaagtt ccacactttt tggaagagta
     3121 ctgagaagat tcagagctat gatctttatc ttaggcagaa agttttttat tatctgatct
     3181 ttgttcaatg agtatttcat atataccacc ctttaaaata aaactatata ttatagagtt
     3241 ctactttttc accaccaccc acttttttta ccataagttc tcctgtagct atgttaaatt
     3301 ctatacttct ggccctgttg ccacctacat cttcagcgat aatccttact cccttttgct
     3361 tgagcttaag cttgacagcc tctatatttc ttttcccaat atccatggtt tggctagaag
     3421 tgccaaacat tgcggctcct cctgcaattt tagcctcaag gttagacgct gatgcgccga
     3481 ttttcaaaag ttcgctgtag atcaagtcta tacctgaatc agcatactta ccgggttttg
     3541 gccctatatt cccagaactg tcaggcaaca tcacatgaac catagcccca atgtttttgg
     3601 ttttatcacg aacacacaca cctacacagg agcctaaccc cagggtaaca atgactatgg
     3661 gattattatc caatgcgtat tcgcctattc cgattatttt tttattcatt atctatcccc
     3721 aaccctattt ttttaagaat cttcttaaga ctttcgttat cgggaatgta gattatataa
     3781 cccgaaatgt ctccgatatc ctcccctacg ttcatactgg tctcaataaa aattataaaa
     3841 tcttctcctt cagtattcat aagggaaact tccgaaacaa tagccgtaag catatcaact
     3901 acgacaccag gaggcattga aaatatattc agtccggtaa atgttgacaa tgacactata
     3961 taagaactgc acattatatt cccaagttca cctattgcag acctagtcat ctcatcaagc
     4021 tgtgtaagat catccggctt agggagcatc atgagttcaa gcatcttttt tatagagtcg
     4081 gttcccataa gaaaaagtat tgatccgttc aattcgccgc caatcccgat atttgctcct
     4141 gccacaattt cttcggggtc tttaaatctc tgccaaatgt gctctatagg tacaattttc
     4201 acatcaggca cacttatttc cacttttctg tctagcatta tggatattga agtagcggca
     4261 ttgccggttc ctatgttccc cagttccttt aacgcatcta gtctggtatt atcaatatcg
     4321 tcgtataaag acactttctt cagcctccca ttaccttctg tttttcttgg tggattctat
     4381 ttcatacctg aaaacaaact taac
//
LOCUS       DAOSHR010000165         4379 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_18069_length_4379_cov_4.535615, whole genome shotgun sequence.
ACCESSION   DAOSHR010000165 DAOSHR010000000
VERSION     DAOSHR010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 4379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4379
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18069_length_4379_cov_4.535615"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            40..705
                     /locus_tag="PLU28_08620"
     CDS             40..705
                     /locus_tag="PLU28_08620"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin fold domain-containing protein"
                     /protein_id="HOG35159.1"
                     /translation="MKKGISFLLILFGAVLTMAATSLKDFSVIDFDNAFKISELTGKK
                     VVVMFTSDDCYYCKKFEDETLTDPTIIQWLKTSFIFTQITSSNIKTAKYNGKTYTYSQ
                     LFGAFGVRGTPTFAFFSKTEYFGTVSGFMDATNFMNILKYLQYYEKNKDVSMADFIKS
                     KTEVPLTKKILSLPQQDIDLLLKIDPNVKAYAPGLDKYTNVIAESSEQAEKLENYLIF
                     IKK"
     gene            826..3246
                     /locus_tag="PLU28_08625"
     CDS             826..3246
                     /locus_tag="PLU28_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HOG35160.1"
                     /translation="MRLNPNDFTEKALQAIQTAQEKTASSHSGTINPEHLLYGILQTD
                     DESVEKLFENNDLTALNRKTEELIYSSTGSTYYSSQNQLYISPALNRVFEISRSESGK
                     LKKKKINLLFLLLGILLEGTSQASKLLSSYTDQNSIYSMLNQTEDSEDSADEDDPIKK
                     YTTDLTKQAKDKKLMPVIGRNEEIERLIEILCRKTKNNPVLIGDPGVGKTAIVEGLAQ
                     RIVSGKVPASLKNKQLLQIDLSSIIAGSKYRGEFEERLKKLTDKVKKSKDSMILFIDE
                     LHNIIGAGSTEGGSMDAANILKPSLAKGELKVIGATTIDEYRKYIEKDKALARRFQVI
                     QVDEPDSEQCIEILKGLKGNYEKHHNVVITDDAVNAAVKLSQRYITDRFLPDKAIDLI
                     DEACSKKSIEFENKPSEVLDLEEKIKRTEDDINDLTIKSAYQEAAYKKALLFELQSEL
                     EKTKKEKNTANSNIVGEEDIAGIIEKWTGIPAKKMLSSERKKLMNLEEEIHKRVISQD
                     QAIKAVAQTIRKARAGLKDPKRPVGSFLFLGPTGVGKTELAKTISEVLFESEDHLIRI
                     DMSEYMEKHSVSRLIGSPPGYVGYEEGGYLTGAVRKKPFSVILFDEIEKAHPDIFNIL
                     LQLLDDGRLTDSQGYVVDFRNTIIIMTSNIGSENILRMLEQGKKEDDIEKQVKEDLKR
                     YFKPEFLNRIDSIVFFRPLNLMNVKSIVDIMIRNLEKLLQEKNIKIEMSEDAKLEVAK
                     NGFDPLYGARPLRRAIEKEIEVPLSEKIISGDINPGDTVKIDISADGTLFIRNGSSQI
                     VKSDTQTS"
     gene            complement(3293..>4379)
                     /locus_tag="PLU28_08630"
     CDS             complement(3293..>4379)
                     /locus_tag="PLU28_08630"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOG35161.1"
                     /translation="MKKKVLKVLSGIILIVIVGLIGFVVYINIQMNKLPGMTFEESFA
                     YTLKDNKNAVVTIGIYKNGESSYEVYGKDGQKLEKEPHVYEIGSLTKTMTAALIARAV
                     NEGRISLEARIDEYLSLPHDKAYPTIHQLLTHTSGYKGYYCELPMIGNFFMGRNSFYD
                     IKDSMILYKLSSKNVSVKKERFEYSNFGYAVLGLVLENIYGKEYTELLNNYLKKDLGL
                     VGTQVSEGKGDLKNYWDWQAGDAYISAGAVISNIDDMLSYLKMQIDEAGEFAECHKAL
                     EKIDKVNGQHQKMGIRTDEIGMAWILDTYNGFIWHNGGTGNFEAYLAFHPNSKTGIVI
                     LSNLKPSERIPASLYGIKLLQELVNSK"
BASE COUNT         1550 a          896 c          774 g         1159 t
ORIGIN      
        1 ggacttttta acaaactaca gataacttga ggtgatacta tgaaaaaagg cataagtttt
       61 ttacttattt tattcggagc ggttttaaca atggcagcaa ccagcctaaa ggatttttct
      121 gtaatagatt ttgacaatgc cttcaagatt tctgaactta ccggtaaaaa agttgtggta
      181 atgtttactt cagatgactg ttattattgc aaaaagtttg aggatgaaac tcttacagat
      241 cccaccatta tacagtggct caaaacatct ttcatcttta cgcagataac ctcctcaaac
      301 attaagaccg caaagtacaa cgggaaaact tatacctact cacagctttt cggagcattc
      361 ggagtaagag gcacgcctac ctttgcgttt ttttccaaga cagaatactt cggaacagtt
      421 tcaggtttta tggatgcaac caactttatg aacattctaa agtacctgca gtactatgaa
      481 aaaaacaaag acgttagcat ggcagacttt ataaaatcca agacagaagt accacttacc
      541 aaaaaaatac tgtctttgcc gcagcaggat attgatctgc ttttaaaaat agatccgaat
      601 gtaaaagcat acgcacccgg tttggacaag tacaccaatg taattgcaga aagctccgaa
      661 caggccgaaa aattagaaaa ctatcttatt tttataaaga aataaccttt actttgaact
      721 tttttgatta tctcaagtct aatatataag gaaagcgtga aaacgctttt cttattacaa
      781 gataattatc agtcaaacat tacaattgat taacgaggtg atactatgag actgaaccca
      841 aatgatttta cagaaaaagc actgcaggcg attcaaaccg ctcaggaaaa aacagcttcc
      901 tcacactccg gaactattaa tccggaacat cttctttacg gtatacttca aaccgatgat
      961 gaaagtgtgg aaaagctctt tgaaaataac gacttgaccg ctcttaaccg aaagacagaa
     1021 gaacttatct attcttcaac aggatctacc tactactcat cgcagaacca gctttatatc
     1081 tctcctgcgc tcaacagagt ttttgagata tcaaggtctg aatccggaaa acttaagaaa
     1141 aaaaagataa accttctttt ccttctttta ggaattttac ttgaaggaac atcgcaggca
     1201 tcaaaactcc tgtcttcata tacagaccaa aacagtatat actcaatgct taaccagaca
     1261 gaagattctg aggattctgc cgatgaagac gatcctataa aaaagtacac tacagacctt
     1321 accaaacagg caaaagacaa aaagctcatg cctgtaatcg gaagaaatga agaaatagaa
     1381 agacttatag aaatactctg tcgaaaaacc aaaaataatc cggttcttat cggcgatccg
     1441 ggagtaggaa aaactgctat tgtcgaagga cttgctcagc gtatcgtatc aggaaaggta
     1501 cctgcctcac ttaaaaacaa gcagttgtta cagatagacc tttccagcat aattgcaggt
     1561 tccaagtaca gaggggagtt tgaagaacgg cttaaaaaac ttacagacaa ggtcaaaaaa
     1621 tccaaagatt caatgattct tttcatcgac gagctacaca acataatagg cgcaggaagc
     1681 accgagggcg gctctatgga tgctgcaaat attcttaaac catcgcttgc aaaaggagaa
     1741 ctcaaggtta taggcgcaac cactattgat gaatacagaa agtacataga aaaagataag
     1801 gcgcttgcac gaagatttca ggtcatacag gtagatgaac ctgactccga acagtgcata
     1861 gaaatactga aaggtcttaa aggaaattac gaaaagcatc ataatgttgt gataaccgat
     1921 gatgctgtaa atgctgcggt gaaactatcc cagagatata taacagacag atttttgcct
     1981 gataaggcca tcgaccttat agatgaagca tgttcaaaaa aaagtataga gtttgaaaac
     2041 aaaccctcag aagttcttga tctagaagaa aaaataaaaa gaactgaaga cgatataaac
     2101 gaccttacta taaagtctgc ctaccaagaa gcagcctaca aaaaagctct tttgtttgaa
     2161 ttacagtcag agcttgaaaa aacaaaaaaa gaaaagaaca cagccaactc aaatatcgta
     2221 ggtgaagagg acattgccgg aattatagaa aaatggacag gtattcctgc taaaaaaatg
     2281 ctctcaagcg aaagaaaaaa acttatgaat cttgaagaag agatacataa aagagtcatt
     2341 tctcaggatc aggctataaa agcggttgca cagactatac gtaaagcacg tgcaggactc
     2401 aaagacccca aacgccctgt cggctctttc ctttttctcg gccctacagg agtaggaaaa
     2461 accgaactgg cgaaaaccat atcagaagtg ctctttgaat ccgaagatca ccttatccga
     2521 attgatatga gcgagtacat ggaaaaacac agcgtatcaa gactgatagg ttctcctccc
     2581 ggctatgtgg gttacgaaga aggaggatac cttaccggtg cggtcagaaa aaaacccttc
     2641 tcggtaatcc tatttgatga aatagaaaaa gcacatccgg atatttttaa tatattgctt
     2701 cagcttctgg atgacggcag gcttaccgac tcacaaggct atgtggtgga cttcagaaac
     2761 actataataa ttatgacaag caacattggc tctgaaaata ttctccgcat gcttgaacaa
     2821 ggaaaaaaag aagatgacat agaaaaacag gtcaaggaag atctcaaaag atactttaaa
     2881 ccggagtttc ttaacagaat agactccatt gtattcttca gacctctcaa tcttatgaac
     2941 gttaagtcaa tcgtagatat aatgatcaga aaccttgaaa aactgctcca ggaaaagaac
     3001 ataaaaattg aaatgagcga ggatgctaaa cttgaagtag ccaaaaacgg atttgacccg
     3061 ctatatggtg caagacctct gagaagggct atagaaaagg aaatagaagt tccgctttcc
     3121 gaaaaaataa tctccggaga tataaacccc ggcgatactg taaaaattga tatatcggca
     3181 gacggaacat tgtttatacg aaacggcagc tcccagatag tgaaatctga tacccagaca
     3241 tcctgaaacc accggccgca tccaaggata aacccttgaa gcggccggat ttttattttg
     3301 aatttactaa ctcctgcaac aatttgatac cgtatagact agccggtata cgttccgacg
     3361 gctttaagtt ggacagaatg acaataccgg ttttactatt tggatgaaag gcaagatatg
     3421 cttcaaagtt accggttccg ccgttgtgcc atataaaacc gttatatgtg tccaatatcc
     3481 aagccatgcc aatctcatct gtacggatac ccatcttctg atgctggccg ttaaccttat
     3541 caattttctc caatgcttta tgacattcag caaactcacc cgcttcatca atctgcattt
     3601 tcaggtagct cagcatatca tctatattag aaattacagc acccgcagaa atataagcat
     3661 ctccggcctg ccagtcccaa taatttttta aatcaccctt accttcagat acctgcgtac
     3721 caacaagtcc taaatccttc ttaagataat tattaagaag ctctgtatat tcctttccat
     3781 aaatattttc aagcacaaga ccaagtacag cgtagccaaa gttagagtac tcaaatcttt
     3841 cctttttaac acttacattt tttgaagaaa gcttgtataa aatcatactg tccttaatat
     3901 cataaaaaga atttctgccc ataaaaaaat tacctatcat aggaagttca caataatagc
     3961 ctttatatcc cgatgtatgc gttagtagct gatgaatcgt aggatatgcc ttatcatgcg
     4021 gcaaagaaag atattcgtca atccttgcct caagagaaat tctcccttca ttaaccgctc
     4081 ttgctatcaa agccgccgtc attgtttttg tcagagaacc gatttcatat acatgcggct
     4141 ccttttcaag tttttgacca tccttcccat agacttcata cgagctttcg ccgttcttat
     4201 agattccgat tgtaacgaca gcgttcttgt tatctttaag agtataagca aaagattctt
     4261 caaatgtcat accgggaagt ttattcatct gaatatttat atagacaaca aaaccaatta
     4321 accctacaat aacaattaaa ataatacccg acaaaacttt cagaaccttc tttttcata
//
LOCUS       DAOSHR010000166         4328 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_18340_length_4328_cov_4.772993, whole genome shotgun sequence.
ACCESSION   DAOSHR010000166 DAOSHR010000000
VERSION     DAOSHR010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 4328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4328
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18340_length_4328_cov_4.772993"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..149)
                     /locus_tag="PLU28_08635"
     CDS             complement(<1..149)
                     /locus_tag="PLU28_08635"
                     /inference="COORDINATES: protein motif:HMM:NF013396.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HOG35162.1"
                     /translation="MKYFFSGIGGIGMSSLALYANYCGHKVYGSNNEKNERVDYLKSK
                     GIEIS"
     gene            complement(161..1219)
                     /locus_tag="PLU28_08640"
     CDS             complement(161..1219)
                     /locus_tag="PLU28_08640"
                     /inference="COORDINATES: protein motif:HMM:NF015022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HOG35163.1"
                     /translation="MKKDKTYNIVIAGGGTGGHYYPALAFVNELSKKYRTRIIYFATK
                     GRIEEKYLPVDIPWAQIVTVDLTGLKRPIYSLENFSVLKKMYSEKLRIKKIIKDFNPD
                     FGFLTGGYVSAPVGLNLRDLKIPFFLHEQNSIVGFTNQKLSKYAQKVFVSFQETVLSN
                     KFLLTGNPVRLPKSSISREYLYTMGIKDPGKRTVLVFGGSLSSEKIDELMLRVYPMIT
                     QTNFIHVTRQPDLFEQFKNVFTREYIPDIYNLLAICDGVICRAGATSIAEIKYFDLKA
                     LLVPWRGAAEDHQFRNAISLEKLGLADIIAEDEKDISKIIGIIEKMPNKGSSFVWNIY
                     EDTSAKKIIDSIEQLIQF"
     gene            complement(1197..2351)
                     /locus_tag="PLU28_08645"
     CDS             complement(1197..2351)
                     /locus_tag="PLU28_08645"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOG35164.1"
                     /translation="MKPVAMKRELMIYFIVLCFTIIIGVLFVYSSLFASELSENINGP
                     DIKFRTHLLGIAIGFFAGFIGFTGADFFIRNKYFMWAMYAFMLGILILALVSKPVNEV
                     HRWFNIGSFQVQPSEYVKFILPAFISFYFLTYVKEKQNIINSFFLPMGLCGIPTVLVF
                     LEPDLSTSAVLFFISFFVCVFNIKNKKIGLVYFIFIFTLIAGAVLFKDQIINNDKFLK
                     PYQLERLAQTEDFQTEQALRALQNGGLLGTAPFGGTMKYNVPVSFSDFIIAVIGEEWG
                     KLGVFVVISLFFFLASQLTRLAYRTSDQAVFSYCATVSLWLVLQSSINAMVGLGVKFI
                     PVTGVTLPLMSYGTNSMVSSIAALGIGMGLIFSSIKKTEEVHSDEKRQDI"
     gene            complement(2348..3637)
                     /gene="murD"
                     /locus_tag="PLU28_08650"
     CDS             complement(2348..3637)
                     /gene="murD"
                     /locus_tag="PLU28_08650"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOG35165.1"
                     /translation="MKVCLVGYGSSNKEMVQFLTEGGNTVFVSNNKPLRPEDIKFLSD
                     RGIMYEEEHSTLLESSELAVVSPGVPPNSSASKIISEKNIRYTTEVEYAWMNIKRANP
                     ACIFVGITGTDGKSTTTSLIGHLLREAGYSTFVGGNIGIPLITAPLNLQFYVAEISSF
                     QLYWGKRFCPEVSVLLNLAPDHLNWHTSLEEYYGCKISMINNTLSSGGLAIVNQNIKN
                     AMYNGKTIHFSGEMLENGYVNFEDRRIKVMNDFLNLKIYSEDTVSAVIACLNLGISEN
                     LIEQGLKSFKPLSHRIELCGNVNGISFYDDSKATNVHSAYNAYLSFRGKGYAALLSGI
                     PKNEDLTELIEEVRNFSKITLVFGAMTEEVKKYPLDEKFVFCNNMEEAVKIGMNALSP
                     GDSIIMSPAGASFDLYQDYKERGNHFKNIAQRFGGDS"
     gene            complement(3639..>4328)
                     /gene="mraY"
                     /locus_tag="PLU28_08655"
     CDS             complement(3639..>4328)
                     /gene="mraY"
                     /locus_tag="PLU28_08655"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOG35166.1"
                     /translation="VGFGSIGFMDDITKILKKQSMGLSAPQKLIMQFAVSFLIYLLIS
                     FFNNHTQMLIPFTLKKLDLGIFYVVWAMVYMTGLSNASNLTDGLDGLAAGVYVISALL
                     TVLVSGLHISLAFTIIFPVLAYVIFNLKPAKVFMGDTGSLALGGIIASIAVYTGTEIF
                     TLFTCFIFLSEMFSVIIQVGSFKLRKGKRVFLMAPIHHHFELLGWHEERVVMNFWIFN
                     IIAGLIALGGR"
BASE COUNT         1320 a          877 c          805 g         1326 t
ORIGIN      
        1 atgcttatct ctataccttt ggacttgagg taatcaacac gttcgttctt ttcattattt
       61 gagccgtaaa ctttatggcc gcagtagttt gcatacagtg caagactgct catgcctata
      121 ccgccgatgc ctgaaaaaaa gtacttcatt attactcctc ctaaaattgt attaactgtt
      181 cgatactatc aataattttt ttagcggatg tgtcttcata aatattccat acaaaagaag
      241 aacctttgtt gggcattttc tctattattc caattatctt tgaaatatcc ttttcatctt
      301 ccgctattat atcggccagc ccaagtttct caagtgaaat ggcatttctg aactgatggt
      361 cttcggccgc accccgccaa ggtacaagaa gtgctttaag atcaaagtac tttatttcag
      421 caatgcttgt tgctcctgct ctgcatataa cgccgtcaca tatggcaaga agattgtata
      481 tatcaggtat atattcgcgt gtaaaaacat ttttaaactg ctcaaaaaga tcaggctgcc
      541 ttgtgacatg gataaagttg gtctgagtga tcataggata aactcttagc ataagttcgt
      601 ctattttttc agagctaagg cttcctccga aaacaagaac cgttctttta cccggatctt
      661 ttatccccat tgtataaaga tactctctgc ttatactgct tttaggaagt cttacaggat
      721 ttccggtaag cagaaattta ttgcttagga cagtttcttg gaaggataca aagacttttt
      781 gcgcatactt tgaaagcttt tggtttgtaa atcctactat agagttttgc tcatgcagaa
      841 aaaaaggtat ttttagatct ctgagattaa gtcctacggg ggcactgacg taacctccgg
      901 taagaaaacc gaaatcagga ttaaaatcct ttattatttt tttgatcctt aacttttcac
      961 tatacatttt ttttaggaca gaaaagtttt caagactgta aatgggtctt ttaagacctg
     1021 taagatcgac tgtgacaatc tgtgcccatg gaatatcaac gggcagatac ttctcttcta
     1081 tacgtccttt ggtagcaaag taaataattc ttgtccggta tttttttgaa agctcattga
     1141 cgaaagctaa agccggatag taatgccccc cggtgcctcc gcctgctatt actatattat
     1201 atgtcttgtc ttttttcatc agaatgtacc tcttccgttt ttttaatact gctgaaaata
     1261 agtcccattc ctatacccaa agcagcaatg gagcttacca ttgagttggt tccatagctc
     1321 atcagaggta aagtaacgcc ggttacaggt ataaatttta ctccaaggcc gaccatagca
     1381 tttattgagg attgaagtac aagccaaaga gatactgtag cgcagtatga aaaaactgcc
     1441 tggtcagaag tcctgtatgc gagtctggtg agctgcgagg ctaaaaaaaa gaaaagggag
     1501 attacaacaa aaacgcccag cttgccccac tcctcaccaa taacggctat tatgaaatca
     1561 ctgaaagaaa caggaacgtt gtacttcatg gttccgccaa agggagcggt accaagaagg
     1621 ccaccattct gaagggctct gagagcctgt tctgtctgaa agtcttctgt ctgtgcgagt
     1681 ctttccagct ggtaaggctt caaaaacttg tcgttgttta ttatctggtc tttgaataaa
     1741 acagctccgg caattaaggt aaagataaaa ataaagtaaa ctagacctat ctttttattt
     1801 tttatattaa aaacacatac aaaaaacgat ataaagaaca gtacggcact tgtgcttaga
     1861 tcaggttcaa gaaagaccaa aaccgtgggt atgccgcaaa gacccattgg caagaaaaaa
     1921 gaattgataa tattttgttt ttcttttacg taggtcagaa aataaaacga tataaacgcc
     1981 ggaagtatga acttgacata ctcagagggc tgaacctgaa aagaacctat attaaaccat
     2041 ctgtgtacct cattaacagg ttttgaaaca agtgccagta tgagaatgcc aagcatgaag
     2101 gcatacatcg cccacatgaa gtacttgttt ctgataaaaa aatctgctcc ggtaaagcct
     2161 atgaatccag caaagaaccc tatagcaata cctaaaagat gtgttctgaa ctttatgtca
     2221 ggaccgttta tattctcaga aagctcactt gcgaaaagcg aactgtaaac aaacagaact
     2281 cctataatga ttgtaaaaca cagtacaata aaatatatca taagttcacg tttcattgca
     2341 acaggcttca agaatctcct ccaaatctct gggctatatt tttgaagtga tttccccttt
     2401 ctttgtaatc ctggtaaaga tcaaagctgg cacctgcagg ggacattatt atgctgtcac
     2461 cgggcgaaag ggcattcata cctattttga cagcttcttc catattattg cagaatacaa
     2521 acttttcatc aagaggatac tttttgactt cctctgtcat tgccccaaag accagggtga
     2581 tttttgaaaa gttcctgact tcttcaatga gttcggtaag atcttcattt ttaggaattc
     2641 cgcttagaag agcagcatat ccttttcccc tgaagctaag ataagcattg tatgcggaat
     2701 gcacattggt ggccttagaa tcgtcataga agcttatacc gtttacattt ccacacagtt
     2761 ctattctgtg ggaaagaggt ttgaagcttt taagcccctg ttctatgaga ttttcactta
     2821 ttcccagatt taaacaggca attacagcgc ttactgtatc ttcactgtat atttttaagt
     2881 ttaagaagtc attcatgact ttaattcttc gatcttcaaa gttgacataa ccgttttcta
     2941 gcatctctcc tgaaaaatgt atggtctttc cgttatacat ggcattttta atattttggt
     3001 ttacaattgc tagtcctccg gatgaaagtg tattgtttat catggatatt ttgcatccgt
     3061 agtactcttc aagcgaggta tgccagttga gatgatccgg agcaaggttc aaaagaacag
     3121 atacttcggg acagaacctt ttcccccagt acagctgaaa agagcttatt tctgccacat
     3181 agaactgaag gtttaatggt gcagttatca gtggaattcc tatattgcct cctacaaaag
     3241 tagaataacc tgcttccctc aaaagatgtc ctataagaga tgtggttgtt gattttccgt
     3301 ctgttccggt tataccgaca aagatacatg ccggatttgc tctttttata ttcatccagg
     3361 catactcgac ttcagtagta taccttatat ttttttcaga tattatcttt gacgcactgc
     3421 tgtttggggg aaccccaggg cttacaactg ccagctcaga gctttcaagc agagtggaat
     3481 gttcttcctc atacattata cctctgtctg aaaggaactt aatatcttca ggacgtaaag
     3541 gcttattatt gcttacaaat acagtgttgc caccttccgt caaaaactga accatctctt
     3601 tattgctgct gccgtaacct acaagacaga ctttcatgtt atcttcctcc aagggctatc
     3661 agccctgcta taatattaaa aatccaaaaa ttcattacaa ctctttcctc atgccagcca
     3721 aggagctcaa aatgatgatg aataggagcc atcagaaata ctcttttacc ctttcggagt
     3781 ttgaaactgc ctacctgtat tataacgctg aacatttcgc tgagaaatat aaagcacgta
     3841 aataaagtaa aaatttctgt tcctgtatat acggcgatag aagcaattat tcctcccagg
     3901 gcaagggaac ctgtatctcc catgaaaact ttggcaggtt tcaggttaaa tatcacataa
     3961 gccagtaccg gaaagattat agtaaaagca agggaaatat gcagtcctga taccagcacc
     4021 gtaagaaggg cagatataac gtatacccct gcggcaagcc cgtcaagtcc gtctgtgagg
     4081 tttgaagcat ttgacagtcc tgtcatatag accatagccc agactacata aaagatgcct
     4141 aggtcaagtt ttttcaaagt aaacggaata agcatctggg tatgattgtt gaaaaaagat
     4201 ataagaagat agataagaaa agaaacagcg aactgcatta tgagtttttg aggagcacta
     4261 agacccatag actgtttttt cagtatcttg gttatgtcat ccataaagcc tatggatccg
     4321 aagcctac
//
LOCUS       DAOSHR010000167         4318 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_18402_length_4318_cov_5.407225, whole genome shotgun sequence.
ACCESSION   DAOSHR010000167 DAOSHR010000000
VERSION     DAOSHR010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 4318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4318
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18402_length_4318_cov_5.407225"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="PLU28_08660"
     CDS             <1..581
                     /locus_tag="PLU28_08660"
                     /inference="COORDINATES: protein motif:HMM:NF012412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35167.1"
                     /translation="MYSSIPVINAGDGGHQHPTQTLTDLLTIRSVKNSLENLTVGCCG
                     DLKFGRTIHSLIKALGRYKNNSFILISPKELKVPEYILNEVLIKNNIQFKQVEKLEDV
                     ISQLDILYMTRVQRERFFNEEDYIRLKDSYILDNEKIKNARQDMIVLHPLPRVNEIAY
                     EIDNDPRAFYFKQAKYGMYVRMALIMKLLGVD"
     gene            583..1014
                     /locus_tag="PLU28_08665"
     CDS             583..1014
                     /locus_tag="PLU28_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HOG35168.1"
                     /translation="MLTITSIKKGIVIDHIKAGTGIKIFHNLNLDKADYKVALIMNVP
                     SKKMGKKDIIKIENEIDIDYAVLGLIDPNITVNIIEDEKITEKRNLQLPERVINIIKC
                     KNPRCITSVEKYINHEFYLSNKSKGEYRCIYCDEVYSVQEN"
     gene            1016..2227
                     /locus_tag="PLU28_08670"
     CDS             1016..2227
                     /locus_tag="PLU28_08670"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HOG35169.1"
                     /translation="MELLIKNVTIIDHDKNIYGDVFIQNGKIKDIQSSISKDCKTVDG
                     MGLILMPSFIDLHSHFRDPGYTYKEDIKSGSMAAVKGGYTYVSLMANTNPICSDMETV
                     RYVIDQAKKIGLIDVHQCISITQGMDGKTLSHLNSIETNIVKMISDDGKGITSNMTMY
                     KAMMIAKEMGLIVNSHVEDKEIAEKDSRLSENIMTIRDIELAKATGCRLHIDHVSTLQ
                     AMQAVVQAKQKGYNNITCEVTPHHIFLTDDTDYKVNPPLRKKEDVTDLINALKYGFVD
                     AIATDHAPHSQEDKQKGFPGISGIETSFSVCYTALVETGYINLNKLSELMSKTPAKIL
                     GLCKGEIKIGNDADLVLVDIKKEVTVNPDTFLSKGKNTPLKGRKLSGEVCMTFKEGKI
                     VYEKEMQNHDN"
     gene            2217..3077
                     /gene="pyrF"
                     /locus_tag="PLU28_08675"
     CDS             2217..3077
                     /gene="pyrF"
                     /locus_tag="PLU28_08675"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR02127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOG35170.1"
                     /translation="MIIDRLYELVAEKGHVCVGLDTCIDYIPKSFSFNFDSIEDTLFY
                     FNKKIIDSTFDVVSCYKVQIAFYEALGIKGLSAYSRTLKYLKSIGAFVIADIKRGDIA
                     STAQMYAKAHFEGDFESDMITLSPYMGMDSLTPYIPYIKEKEKGAFVLVKTSNPGSKD
                     IQFLTCENGTKVYEEVANKIIENGRELCGKYNFSSIGAVVGCTNLEQAISIRKQMENT
                     FFLIPGYGAQGGTAADIKHLMINKNGGVVNSSRAILLAHKNPMFVDISFDMAARKEAL
                     KMRSEIADAL"
     gene            3067..3810
                     /locus_tag="PLU28_08680"
     CDS             3067..3810
                     /locus_tag="PLU28_08680"
                     /inference="COORDINATES: protein motif:HMM:NF000798.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HOG35171.1"
                     /translation="MPYEYLKSNIILNCKVSEDIFEMKVEFKSNTEPGQFFMLRKNDS
                     PTFLSRPLSVYYADKETISFLYQVKGKGTQLFSLMNPGEKIDIMGPLGNSFSTAFCGK
                     KTAVISGGIGIAPMVYLAQELLKRDNSVDFYCGFKGMPYCTDILDKKIKVFVSSQEGV
                     AGHHGLITEIFNPSAYENVMTCGPVPMMKIVANMCKEKNIPCQVSMEQHMACGMGACL
                     VCTCETEHGIKRVCKDGPVFDGKELIMND"
     gene            3803..>4318
                     /locus_tag="PLU28_08685"
     CDS             3803..>4318
                     /locus_tag="PLU28_08685"
                     /inference="COORDINATES: protein motif:HMM:TIGR01037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HOG35172.1"
                     /translation="MTKTKICGINFKNPVIAASGTFGFGREFSQAYDLSKLGGICTKG
                     LTFNPRQGNKGIRCCETASGMINSVGLQNPGIQSFLDNDLAFMSSLNTVIIANLSGSS
                     IQEYVQGAELLNSSNVDMIELNISCPNVKEGGMNFGIKTQSAVQVLGQVRKAVSKPLI
                     VKLSPNAEDIVD"
BASE COUNT         1557 a          840 c          864 g         1057 t
ORIGIN      
        1 caatgtactc ttctattcca gtaatcaatg ccggtgacgg cggtcatcag caccctacac
       61 aaactcttac cgatcttcta accatccgtt cagttaaaaa ctcccttgaa aatcttacag
      121 taggttgttg cggcgatctt aagttcggac ggacaattca ctcactcata aaagctctgg
      181 gccgctacaa aaacaactcc tttatactta tctcacctaa agaactaaaa gtcccggaat
      241 atatcctgaa tgaagtcctt atcaaaaaca atatacagtt caaacaagta gaaaaacttg
      301 aagatgttat ttcacagctt gatattcttt atatgacaag ggtacagaga gaacgttttt
      361 tcaatgaaga agattatata cggctaaaag acagttatat ccttgataat gaaaagataa
      421 aaaacgcccg tcaggacatg atagtccttc atcctctgcc aagggtaaat gaaatagctt
      481 atgaaataga caacgatccc agagctttct acttcaaaca agcaaaatac ggtatgtatg
      541 taagaatggc acttataatg aaattattgg gggttgattg atatgcttac tataacaagt
      601 ataaagaaag gtattgtgat agaccacata aaagccggta caggaatcaa gatcttccat
      661 aacctcaacc ttgataaagc agattacaaa gttgccctta taatgaatgt accaagcaaa
      721 aaaatgggaa aaaaggatat aataaagata gaaaatgaaa tagatattga ttacgcagtt
      781 cttggactca tagacccaaa cataaccgtc aacatcattg aagatgaaaa aataacagaa
      841 aaaagaaatc ttcagcttcc cgaacgtgta ataaatatta taaaatgcaa aaatccaaga
      901 tgcataacat cggttgaaaa gtacataaat catgaatttt atctttcaaa caaatcgaaa
      961 ggagagtatc gatgcatcta ctgcgacgaa gtatactcgg tacaggaaaa ttaacatgga
     1021 acttctgata aaaaacgtta ccattattga ccacgataag aacatctacg gcgatgtttt
     1081 tatacagaac ggaaaaatta aagatattca gagcagcata tccaaagact gcaaaactgt
     1141 tgatggcatg ggtcttatac tcatgccgtc gtttatagac ctgcactctc acttcagaga
     1201 cccaggatac acatataaag aagatataaa aagcggatca atggctgcgg taaagggcgg
     1261 atacacctac gttagcctca tggccaacac aaatcccata tgctcggata tggaaacggt
     1321 cagatacgtt attgaccagg caaaaaagat aggacttatt gatgtacatc agtgcataag
     1381 cataactcag ggtatggatg ggaaaacctt atcacatctg aacagcatcg aaacaaacat
     1441 agtaaaaatg atttcagatg acggaaaggg tattacaagc aacatgacca tgtataaagc
     1501 catgatgatc gcaaaagaaa tgggacttat tgtaaactca catgttgaag ataaagaaat
     1561 agcggagaaa gattccagac tatctgaaaa cataatgacc atacgggata ttgagcttgc
     1621 caaagccaca ggctgccggc tccacataga tcatgtaagc actttgcagg ctatgcaagc
     1681 agttgttcag gcaaaacaaa aaggatacaa caatataacc tgtgaagtaa ccccacatca
     1741 catatttctg acagatgata cagactataa agtcaatccc ccactgcgga aaaaagaaga
     1801 tgttactgac cttataaatg ccttgaagta tggttttgta gatgcgattg caaccgatca
     1861 cgccccacat tcccaagaag ataaacaaaa aggattccca ggaatttcag gcatagaaac
     1921 atctttttca gtatgctata ccgcactggt tgaaacagga tacattaacc tgaacaaact
     1981 ttctgagctg atgtcaaaaa cacctgcaaa aatactggga ctgtgcaaag gagaaataaa
     2041 gataggaaac gatgcagatc ttgtccttgt agacatcaaa aaagaagtaa ccgttaatcc
     2101 agacaccttc ctatcaaaag gaaaaaatac tccgctcaaa ggcaggaaac tttccggaga
     2161 agtctgcatg acttttaaag aaggaaaaat agtatatgaa aaggagatgc aaaaccatga
     2221 taattgacag actgtacgaa ctcgtggccg aaaaaggaca cgtatgcgtg gggcttgaca
     2281 cttgcattga ttacattccg aaaagttttt cctttaactt tgattcaata gaagatactt
     2341 tattctactt caataaaaag ataatagact caacctttga tgttgtttca tgctataaag
     2401 tacagatagc attttacgaa gcacttggta taaagggact ttcagcctac agcagaactc
     2461 tgaagtacct taaaagcatt ggagctttcg ttatagccga tataaaaaga ggcgatatag
     2521 cctcaacagc ccagatgtat gcaaaggcac atttcgaagg cgactttgaa tccgacatga
     2581 taacactcag tccttacatg ggcatggaca gcctcactcc gtatattccg tacataaaag
     2641 aaaaagaaaa gggagcattt gtcctagtga aaacatccaa tccgggctca aaggacatac
     2701 agttccttac atgtgaaaac ggaacaaaag tatatgaaga agttgccaac aagattatag
     2761 aaaacggcag ggagttgtgc ggaaagtata atttcagctc cataggcgca gttgtcgggt
     2821 gcaccaatct tgaacaagcc ataagcataa gaaagcagat ggaaaatacc tttttcctta
     2881 tacccggtta tggtgcccag ggaggaacgg cagctgatat aaaacacctt atgatcaata
     2941 aaaacggcgg cgttgtaaac tcatcaagag ccatactgct tgcacataaa aacccgatgt
     3001 tcgttgatat ttcttttgat atggcagcaa ggaaagaagc gttgaagatg aggagcgaaa
     3061 tagcagatgc cttatgagta tcttaagagc aatataattt taaattgcaa agtatccgaa
     3121 gatattttcg aaatgaaagt tgaatttaaa agcaatacgg aacccggaca gttttttatg
     3181 ctgagaaaaa atgacagtcc cacatttctt tcgcgcccac tcagtgtgta ttacgcagac
     3241 aaagagacaa tctcattcct ttaccaggtt aaaggaaaag gaacacaatt attttctttg
     3301 atgaaccccg gggaaaaaat agacataatg ggacctctgg gaaactcctt cagcactgcc
     3361 ttctgtggaa aaaagacagc tgtcataagc ggcggcatag gcatagctcc gatggtatat
     3421 cttgctcagg agcttctaaa aagagataat tccgttgatt tttactgcgg cttcaagggc
     3481 atgccttact gcacagatat tcttgacaaa aagattaaag tctttgtatc ttcacaggag
     3541 ggagttgcag gacaccacgg acttataacc gaaattttca acccctcggc ttatgaaaat
     3601 gttatgacct gtggacccgt acctatgatg aagatagttg caaatatgtg caaagaaaaa
     3661 aatattccct gccaggtctc aatggaacaa catatggcct gtggcatggg ggcatgcctg
     3721 gtatgcacat gtgaaacgga acacgggata aagagggtct gtaaagacgg ccctgtattt
     3781 gatggaaaag agctgattat gaatgactaa aaccaagatc tgtggtataa acttcaaaaa
     3841 tccggtaatt gcagcatccg gaactttcgg attcggcaga gagttttcac aggcatatga
     3901 cctgtccaaa ctcggaggca tctgcaccaa aggacttacc tttaatccga gacagggcaa
     3961 caaaggaata cgctgctgtg aaacagcaag cggaatgatc aacagtgtcg gactacaaaa
     4021 tcccggaata caaagctttc tggacaacga ccttgctttt atgagctctc ttaatacagt
     4081 tataatagct aacctcagcg gttccagcat acaagagtat gtccagggag cagagcttct
     4141 taacagttcg aatgtagata tgatagaact caacatatca tgcccgaacg tcaaagaagg
     4201 cggtatgaac ttcggaataa agacccaaag cgctgtacaa gtactcggac aggtaagaaa
     4261 ggcagtttca aaacctttga ttgtaaaact ctctcctaat gctgaggata tagtagat
//
LOCUS       DAOSHR010000168         4290 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_18559_length_4290_cov_4.831641, whole genome shotgun sequence.
ACCESSION   DAOSHR010000168 DAOSHR010000000
VERSION     DAOSHR010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 4290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4290
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18559_length_4290_cov_4.831641"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..787)
                     /locus_tag="PLU28_08690"
     CDS             complement(<1..787)
                     /locus_tag="PLU28_08690"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOG35173.1"
                     /translation="MKKLVLIALLVSLSAIAIFGAKLVVWTSEKQVPFMKKMGEQFTK
                     KYGVTLDVQMVNFGDIKSKFLTAAQAGEGPDIFVLAHDNMGELVANGLVEPFEFSAVD
                     RKNYYDVAIDAFTMNGKLYGIPYAIESILMFYNKDYIKDPPKTAEQLLKYQKDFTTKD
                     TFGLLWDVNNLYFSYPFLSGFGGYVFKWDGKQYNVKDVGLANAGAIKGGNLIKTIIEN
                     GMPSSSNYGTMDSAFKDGLCAMIINGPWAIPDYNNAGINYGVWP"
     gene            complement(885..>2237)
                     /locus_tag="PLU28_08695"
     CDS             complement(885..>2237)
                     /locus_tag="PLU28_08695"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase subunit B"
                     /protein_id="HOG35174.1"
                     /translation="RDGQQSLIATRMKTSEFSDVLGTFDNIGFYSMEVWGGATYDSCI
                     RFLDEDPWERLSILRKGLKNTKIQMLLRGQNLVGYRHYSDDVVELFIKKMADYKMDIV
                     RVFDALNDIRNLEKSIETAKKYKMEVQAAISYTVSPVHNLEFYRSYAKELVERGIDSI
                     CIKDMAGLITPKQAYELIKDLKSNFNIPIQLHSHNTAGLADLSYYAAVEAGVDVLDLA
                     LSPFAMGSSQPALEPFNHSMEMGLDGAGIIKLVDHFWKVRKNHAADDVNMQSINSKML
                     LSQIPGGMISNLVSQLKQQKAEDKLDLVMEEVPRVRKDLGYPPLVTPTSQIVGVQATL
                     NVLTGERYKMITNEVKNYMKGEYGRSPAPVDPQLLKKALGDEKPITHRFADDIAPELE
                     KARKESGVLALTDEDLLTYVLFGEVGKKFLTKKYEKEIKVDQDILKKYKNEEVSIYPV
                     "
     assembly_gap    2238..2337
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2438..2575
                     /gene="rpmH"
                     /locus_tag="PLU28_08700"
     CDS             2438..2575
                     /gene="rpmH"
                     /locus_tag="PLU28_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008650850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOG35175.1"
                     /translation="MKRTYQPSKVKRARTHGFLERSKTSGGRAVLRRRRARGRKRVSV
                     Q"
     gene            2579..2932
                     /gene="rnpA"
                     /locus_tag="PLU28_08705"
     CDS             2579..2932
                     /gene="rnpA"
                     /locus_tag="PLU28_08705"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOG35176.1"
                     /translation="MKKMSFRQRERLRLKNDFDSLFDTGGRVESESFVIVYKANCKGC
                     CRLGFSIRKKFGKAHLRNRLRRCLKEIYRTNKPLFPTSMDIIFIPRKGLSKRFLQVSY
                     RELESIVLGMIEALR"
     gene            2929..3168
                     /gene="yidD"
                     /locus_tag="PLU28_08710"
     CDS             2929..3168
                     /gene="yidD"
                     /locus_tag="PLU28_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOG35177.1"
                     /translation="MKYLFLGIIRFYQRFISPLKPATCRFYPTCSAYTYEAVKRFGSI
                     KGLYLGLRRISRCHPFNPGGIDPVPENFSFFKKKR"
     gene            3193..>4290
                     /gene="yidC"
                     /locus_tag="PLU28_08715"
     CDS             3193..>4290
                     /gene="yidC"
                     /locus_tag="PLU28_08715"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HOG35178.1"
                     /translation="MKKTVTLILFIMLCLSVFALPEYRITQDPTSQSVTLEMRLYKVT
                     VDNYGRIQDYSIINTQTKSMVSVYKYTNDSYDLLDSDGKEMLPVSFEVKESEDKSKVE
                     FVYGFENGGTKGYIFHNNPNFEFDLSVKGLSGTVVLPNISLPGKVSSFSVENLSSYGI
                     SSATNSYVSYKAKPFKISLIVSSDVPIDRKSLTVSFTGKLDLKNFMGPMKLTLIRELY
                     QGEEYKTVNGLLKDVKAISWTTVIFYWFVEFLWWLNNVTGNFGWAIIIFTLIINVIFL
                     PLYMKQKKSMIRMKELQPELDKIKKKYPNNPQKMQEAQMALYKEKGFNPASGCLFALI
                     QLPIFIVLWQVIQYFGESFAYNPRFLIWQDLS"
BASE COUNT         1229 a          824 c          819 g         1318 t
ORIGIN      
        1 gtggccatac tccgtagttt ataccggcat tgttgtagtc cgggattgcc catgggccgt
       61 tgattatcat agcgcaaagt ccgtctttga aagctgaatc catagtacca tagtttgaag
      121 atgatggcat tccgttttca attatcgttt taataaggtt tccgcctttt atagcaccgg
      181 cgtttgcaag acctacatcc ttaacattat actgttttcc gtcccacttg aaaacatatc
      241 ctccgaatcc tgagaggaaa gggtatgaaa aataaaggtt gtttacatcc cagagaagac
      301 cgaaggtatc cttggttgtg aaatctttct ggtatttgag gagctgttcg gctgtttttg
      361 gcggatcttt tatataatcc ttgttataga acatcagtat agactcgatt gcataaggga
      421 taccgtagag tttgccgttc attgtgaaag catctattgc tacatcgtaa tagttctttc
      481 tgtcaacagc ggaaaattca aaaggttcga ccagaccgtt agctaccagt tcacccatat
      541 tatcatgagc aagaacaaat atatccggtc cttctccagc ctgcgcggcg gtaaggaact
      601 ttgactttat atctccgaaa ttgaccatct ggacatcgag tgtaacaccg tactttttgg
      661 tgaactgctc gcccattttc ttcatgaacg gaacctgctt ttcggatgtc catactacca
      721 acttagcgcc aaatattgca atagcgctta aagaaaccaa caatgctatg agaactaact
      781 tcttcatact tttaccccct tttcactctt ttagatagaa ttactacata acaaattata
      841 ccataaaatt aatttaagtt taactctcca aacgatttta ttttttacac ggggtatatg
      901 cttacttctt catttttata cttcttgagt atatcctgat ctactttaat ttctttttca
      961 tattttttgg taagaaattt ctttccgact tcaccgaaaa gaacgtatgt gagaagatcc
     1021 tcatccgtca atgcaagtac tccactttcc tttctggctt tttcgagttc aggggctata
     1081 tcatctgcga atctgtgggt gatgggtttt tcatcgccaa gagccttttt tagaagctgt
     1141 ggatcaacag gagccggcga ccttccatac tcgcctttca tatagttttt aacctcattg
     1201 gttatcatct tatatctctc gcccgtcagg acatttaagg ttgcctgaac gccgacaatt
     1261 tggcttgtag gagtaacaag aggaggatag ccaagatctt ttcgcactct cggaacttct
     1321 tccataacca ggtcaagctt atcttcagct ttttgctgtt tcagctggga cactagattg
     1381 gagatcattc ctcctggtat ctgagaaaga agcattttgc tgtttatgga ctgcatattt
     1441 acatcatctg cagcatgatt ttttctaacc ttccagaaat gatcaacaag cttaattata
     1501 cctgctccgt ccagtcccat ttccattgaa tggttaaaag gctcaagtgc cggctgggaa
     1561 gatcccattg caaaaggact aagtgcaagg tcaagtacat ccactcccgc ttcaactgcc
     1621 gcatagtatg aaagatcagc cagtccggca gtattatgtg aatgcagctg tatgggaata
     1681 ttgaaattgg atttaagatc tttaataagc tcgtatgcct gtttaggagt tataagcccg
     1741 gccatatctt ttatacatat tgaatctatg cctctttcta caagttcttt agcgtatgat
     1801 ctgtaaaatt caagattgtg gacaggactc actgtatatg aaatggctgc ctgaacttcc
     1861 attttgtatt ttttggctgt ttctattgat ttttctaggt ttcttatatc gttcagagca
     1921 tcaaataccc ttacgatgtc cattttataa tcagccattt tctttatgaa aagctctaca
     1981 acatcatctg agtaatgtct gtagcccaca aggttctgtc cacgcagaag catctgtatt
     2041 ttggtgtttt taagaccctt tcgcagaata ctcagcctct cccacggatc ttcatcaagg
     2101 aaacggatac aactgtcata agtggctcct ccccagactt ccatagaata gaaacctata
     2161 ttgtcaaatg tgcccagtac atcagaaaat tcagaagttt tcatccttgt ggctatgagt
     2221 gactgctgac cgtcacgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncct
     2341 ttaaaaattt gttacttcta tttattgaca gaaaaacctt ttacggttat aattaaggat
     2401 agtcttttta atcaatatac ccaaaggagg tacggcaatg aaaagaacat atcagccttc
     2461 aaaagtaaaa agagccagaa cccacggttt tcttgagaga tcaaaaacca gcggaggaag
     2521 agcagtttta agaagaagaa gagccagagg aagaaaaaga gtgtcagtcc agtaaaaaat
     2581 gaaaaagatg tcattcagac aacgggaacg tctgagacta aaaaatgact ttgattctct
     2641 cttcgatacc ggaggaaggg tagaatcaga gagttttgtg attgtctata aggccaattg
     2701 taaaggttgt tgcagattag gcttttccat aagaaaaaag tttgggaaag cccatctgcg
     2761 gaatcgatta agaagatgcc tcaaggaaat ttacagaact aacaaaccat tgtttccaac
     2821 aagcatggat atcatcttta taccaaggaa gggtctgtca aaaagatttt tacaggtctc
     2881 ttatcgtgag cttgaaagta ttgttttagg catgatagag gctttgagat gaagtacttg
     2941 tttcttggaa taatcaggtt ttaccagcgc tttatatctc ctttaaaacc tgctacctgc
     3001 aggttttatc ccacatgctc ggcttatacc tatgaggctg tgaagaggtt tggaagtatc
     3061 aagggcttgt atttaggtct caggcggatt tcacgttgtc atccttttaa ccctggcggc
     3121 atagacccgg tacctgaaaa ttttagtttt tttaaaaaaa agaggtaaac tttataaaaa
     3181 ggggtgcttg atttgaaaaa aacagtaact ttaatacttt ttattatgct gtgtctgtcc
     3241 gtattcgcat tgcctgaata caggataacc caagacccga caagccagag cgtgactttg
     3301 gagatgagac tttataaggt caccgttgat aattacggaa ggattcagga ttattcgata
     3361 ataaataccc aaactaaaag catggtatca gtttataagt acacaaacga cagttatgat
     3421 cttttagatt ccgacgggaa agagatgctt cctgtttctt ttgaagtcaa agaatcggaa
     3481 gataaaagca aagttgaatt tgtctatggg tttgaaaatg ggggaacaaa aggttatatc
     3541 ttccataata accccaactt tgagtttgac ctttcggtaa aggggctttc aggaactgta
     3601 gttctcccca atatctccct ccccggtaaa gtttcttcgt tttcagttga gaatctaagc
     3661 tcatacggca ttagttcagc aaccaattct tacgtttcat ataaagcaaa accgtttaaa
     3721 atttcactca tagtatcttc tgatgttcct atcgacagga agagccttac cgtaagtttc
     3781 actgggaagt tggatcttaa aaattttatg ggtccgatga agcttacttt gataagggaa
     3841 ctctatcaag gcgaggagta caaaactgtt aacggcctat tgaaggatgt aaaagccata
     3901 agctggacta cagtcatatt ctactggttt gttgagtttt tgtggtggct taataatgta
     3961 actggtaact ttggttgggc tataataata tttacactta tcataaatgt tatcttcctt
     4021 ccgctttaca tgaagcagaa aaaatcgatg ataagaatga aggagcttca gcccgagctt
     4081 gacaaaataa agaaaaagta tcctaacaat ccgcagaaaa tgcaggaagc ccagatggca
     4141 ctttacaaag aaaaaggctt caatcctgca agcggctgtc tttttgcttt aatacagctt
     4201 ccaatattta ttgtgctttg gcaggtcata cagtattttg gagaaagttt tgcttacaac
     4261 cctagattcc ttatctggca ggatctttct
//
LOCUS       DAOSHR010000169         4278 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_18633_length_4278_cov_9.838503, whole genome shotgun sequence.
ACCESSION   DAOSHR010000169 DAOSHR010000000
VERSION     DAOSHR010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 4278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4278
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18633_length_4278_cov_9.838503"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="PLU28_08720"
     CDS             <1..660
                     /locus_tag="PLU28_08720"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOG35179.1"
                     /translation="PLYIEKYLYRTPVEVIKRFPEYYNIAFSESKVNFSNRVFDVLIS
                     SLVLILSSPIILLSIILIKLEDGKEIFFKQKRIGMNGKSFYAYKLRTMNRVQENGEYV
                     DKPTKIGNILRKFRINELPQIINVFNGDMSLVGPRPDVERTMFFCEEEIQFYHFRRTV
                     PQGITGNAQVLYKYVDKLEKETFSKRLSYDIYFVKNYSFSLYFTTLLRTVETVAFGRG
                     K"
     gene            665..1501
                     /locus_tag="PLU28_08725"
     CDS             665..1501
                     /locus_tag="PLU28_08725"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOG35180.1"
                     /translation="MIYSINLEEWIDKNFLNQIENYKNLQNLFNKSIVQSIIENEDND
                     SATLLIGYYNPFSYSYIGQIMDSIELLVAFKKISKIIYFSSFGVYKPKFEGKYREDDT
                     VQPYNSIGLNALLYEDFFTFLNTRYSTDIYILRNFSIYGPMQNDISLIPNLMKGFIKN
                     EEVYIGDMKKVRDYLYIEDFIFLVKKMIEEKKSHEKKVYNIGSGKGTDIKTLINIMNC
                     ISNRKVNAIFDPSKIRKEYDYDYVVADIEKISTDYGFSQKISLEEGLKLTYQWASGRV
                     CI"
     gene            1498..2211
                     /locus_tag="PLU28_08730"
     CDS             1498..2211
                     /locus_tag="PLU28_08730"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOG35181.1"
                     /translation="MMKNSIILAGGLGTRLRPLTDEIPKPLLPIGDKPILERIIENMT
                     AQGIENIFISVNYKKDMIKNYFKDGKMMKTKIKYIEEERSTGTAGCLTLLPENFCDDI
                     IVSNGDLLSDVDYNGIDKLLHVDGADFVLTTIEKSYKIDFGVINSDESTKKLIDWVEK
                     PTYTYNVNAGIYGVSAKVIKFIKNNFKQNTRIDMPELWKILTENNFTLLIYKHPGDWY
                     DIGRIDDYVNINSTFSNNN"
     gene            2224..3195
                     /locus_tag="PLU28_08735"
     CDS             2224..3195
                     /locus_tag="PLU28_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent 4,6-dehydratase LegB"
                     /protein_id="HOG35182.1"
                     /translation="MKVLVTGAGGFIGSHLTEMLVQKGFTVKAFVRYNSKNNWGWLES
                     SEYKKEIEVYTGDIRDFDSVNDAVKGTDAVFHLAALIGIPYSYVSPLAYIKTNTEGTY
                     NVLQAARNNNVSRIVHTSTSEIYGTAQYVPIDEKHPYNPQSPYAASKTGADHMALSYY
                     RSFGTPVTVIRPFNTYGPRQSARAVIPTIISQIMAGKKEIKLGNLLPTRDLNYVKDTA
                     AGFIHVGLNEHTIGDVYNLGTGKEISIGDLAQKIIELIGTQVSIKTDNQRLRPEKSEV
                     ERLLSDPSKTFRVTGWKSAYTLEEGLRETISWIEKNIDVFKTDLYNV"
     gene            3209..>4278
                     /locus_tag="PLU28_08740"
     CDS             3209..>4278
                     /locus_tag="PLU28_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOG35183.1"
                     /translation="MQEILLDAPNLGNVEREYLLKCLDSTFVSTAGPFVPEFEEKFAA
                     FTSSKRAVACQSGTAAIYMSLYEAGIKPGDEVIVPVITFIASVTPIVHIGAVPVFVDV
                     DINTWTIDPSKIEEHITSKTKAIIPVHIYGNPCDMDKIKEIADKHNLKIIEDATESLG
                     ATYKGIMTGTFGDYGCFSFNGNKVITTGGGGMIVSDKEKEMDHIKFLINQARDASKGY
                     YHPEVGFNYRMTNLEASLGLAQFSRLPEFLSKKRGYAEIYKNAFKDYTNIKFQQTLPQ
                     CESSYWLPSAVVESKHTIPEIQQLLKEKGIPTRRIFMPIVEFPPFMKYKQESYANAYY
                     IYENGINLPGSTVNSFEQTEYIA"
BASE COUNT         1555 a          728 c          809 g         1186 t
ORIGIN      
        1 ccgctttata tagaaaaata cctctacagg acgcctgttg aagtgataaa aagatttcca
       61 gagtattata atatcgcttt ttcggaatct aaagttaatt tttcaaacag agtatttgat
      121 gtactcataa gttctcttgt tttgattcta tcttccccca taattctttt aagcataatt
      181 ttgattaagc tggaggatgg aaaggaaata ttttttaaac aaaaaagaat aggaatgaac
      241 ggaaaatcat tttatgccta taagctccgt acaatgaaca gagtacaaga gaacggagag
      301 tatgttgata aacctacaaa aatcgggaat attttaagaa aattcagaat aaacgaactt
      361 ccacagatta taaatgtttt taatggggat atgtctcttg taggtcctag gccagatgtt
      421 gaaagaacca tgttcttctg tgaggaggag atacagtttt atcatttcag aaggactgtt
      481 ccgcagggaa ttacaggaaa tgcacaggtt ttatataaat atgtggataa attggaaaaa
      541 gaaacatttt caaagcgttt gagctacgat atatattttg taaaaaacta ctctttttca
      601 ctgtacttta caacactttt aagaacagtg gaaaccgttg cgtttggaag ggggaagtaa
      661 tgtaatgata tactccataa acctggaaga atggatagat aaaaactttt taaatcagat
      721 tgaaaattac aaaaatctcc agaatctttt taataaaagt attgtacaga gtatcataga
      781 aaatgaagat aacgattcag ccacattact cataggatat tataaccctt tcagctattc
      841 atatataggg cagatcatgg actctataga gcttcttgta gctttcaaaa aaatctccaa
      901 aatcatttat ttttcatctt ttggagtata taaacccaaa tttgagggaa agtaccgaga
      961 agacgatact gttcagccat ataattcaat aggtctaaac gcactacttt atgaggactt
     1021 ctttaccttt ctgaatacaa gatactccac ggatatctat atactcagaa atttcagcat
     1081 atatggacct atgcagaacg atatttccct cattccaaac ctgatgaaag gttttataaa
     1141 gaacgaagaa gtatatatag gcgatatgaa aaaagtacgt gattatctct atatagaaga
     1201 ctttatcttt ctggtaaaaa agatgatcga agaaaaaaaa tctcatgaaa aaaaggtata
     1261 taatatagga agcggaaaag gtacggatat taaaaccctt ataaacatta tgaactgtat
     1321 ttccaacaga aaagtaaatg caatattcga tccttcaaag atccgaaaag aatatgacta
     1381 tgactatgtt gtggcagata tagaaaaaat ctctaccgac tatggatttt cacagaagat
     1441 tagtcttgaa gaaggcctta aacttactta ccaatgggca agcgggagag tatgcatatg
     1501 atgaaaaact ctattatact tgccggaggt cttggaacgc gtcttcgtcc ccttacggat
     1561 gaaataccca agccgctgct tccaatagga gataaaccta ttttggagcg gataatagag
     1621 aatatgacag ctcagggcat agaaaatata tttatttccg tcaattacaa aaaagatatg
     1681 ataaaaaact actttaaaga cggaaaaatg atgaaaacta aaataaagta cattgaagaa
     1741 gaaagatcaa ccggaaccgc cggatgcctt accctcttac cagagaactt ttgcgatgat
     1801 attatagtat caaacggaga tcttctgtct gatgttgact ataacgggat agataagctc
     1861 ctgcatgttg acggagcaga ttttgtgctt accacaatag agaaaagcta taaaattgat
     1921 tttggagtta taaactctga tgaatccacg aaaaaactta tagactgggt tgaaaaaccg
     1981 acttacacat ataatgtaaa tgctggaata tatggagtat ccgcaaaagt tatcaagttt
     2041 ataaaaaaca atttcaaaca gaatacacga attgatatgc ctgaactatg gaaaatactc
     2101 actgaaaata attttacact tcttatatat aaacatcccg gtgattggta cgacataggg
     2161 agaatagacg attatgtaaa cataaacagc accttttcca acaacaacta acgggaggta
     2221 tttatgaaag tacttgttac aggagcaggg ggattcatag gttctcatct taccgaaatg
     2281 cttgttcaga aaggctttac agtaaaggcc tttgtaagat ataactccaa aaataactgg
     2341 ggctggcttg aaagctcaga atacaaaaaa gaaattgaag tatatactgg cgatataaga
     2401 gattttgatt ctgtaaatga tgcagtaaag ggcacagatg cagtatttca ccttgctgcc
     2461 cttataggaa ttccatattc gtatgtatcg cctctggcat atataaaaac caatacagaa
     2521 ggaacgtaca atgttcttca agctgcaaga aataacaatg tttcacgcat agtccatacc
     2581 tccacaagcg aaatatacgg aaccgcacag tatgttccca tagatgaaaa acacccttac
     2641 aatccccagt ctccatatgc tgcaagtaaa acaggagcag accatatggc gctttcatat
     2701 tacagatcct ttggaacacc tgttaccgta atacgacctt ttaatacata cggtcccaga
     2761 cagtcggcac gggcagttat tcctactata ataagccaga taatggctgg taaaaaagaa
     2821 ataaaacttg gaaatcttct tcccacaaga gatcttaatt atgtaaaaga tacagccgcc
     2881 ggttttattc acgtaggtct taatgaacat accataggcg atgtgtacaa tctcggaaca
     2941 ggaaaggaaa taagcatagg tgatcttgca caaaaaataa tagaacttat aggaacacaa
     3001 gtaagtatta aaacagataa tcaaagactc aggcctgaaa aatcagaggt tgagagattg
     3061 ctcagcgacc cttcaaaaac ttttagagtg acaggctgga agagtgctta tactcttgaa
     3121 gaagggctta gagaaacaat ttcttggata gaaaaaaaca tagatgtttt taaaaccgat
     3181 ctgtacaatg tatgaggaga tgacaagaat gcaggaaata cttttggatg ctcccaatct
     3241 gggaaatgtc gagagagagt accttcttaa atgccttgac agtacgtttg tctcaacagc
     3301 ggggcctttt gttcctgagt ttgaagagaa atttgcagct tttacctcaa gcaaacgggc
     3361 agtagcctgt cagagcggaa cagcagcaat atatatgtcg ctgtatgaag ccggaataaa
     3421 gcctggcgac gaagttatag tacccgtaat tacatttatt gcttcggtga ctcccattgt
     3481 gcatattggg gcagttccgg tatttgtgga tgtagatatt aatacatgga ccattgatcc
     3541 ttccaagatt gaggaacata taacttccaa aaccaaagcc attatccctg tccatatata
     3601 tggaaatcca tgcgatatgg ataagataaa agaaattgca gataaacata atcttaaaat
     3661 catagaagat gcaaccgaaa gccttggggc tacgtataaa ggcatcatga ccggcacatt
     3721 cggagattac ggctgttttt cgtttaacgg caacaaggtc attacaaccg gaggaggagg
     3781 aatgatcgtc tctgataaag aaaaagagat ggatcatata aaatttctta taaatcaggc
     3841 cagagatgca agtaagggct actatcatcc tgaggtagga ttcaattacc gtatgactaa
     3901 tcttgaagct tcacttggat tggctcaatt ttcaagactg cctgaatttt taagcaaaaa
     3961 aagaggatac gctgaaattt ataaaaatgc ctttaaagat tataccaata taaaattcca
     4021 gcagactctt ccccagtgtg aaagttctta ttggctgcct tcagcagtgg tagaatccaa
     4081 acacaccata cctgaaatac aacagctttt gaaagaaaaa gggataccaa cgagaagaat
     4141 ctttatgcca atagtagaat ttccgccttt tatgaagtat aaacaggaat cttatgcgaa
     4201 tgcttactat atatatgaaa atggaataaa tcttccaggt tctacagtta actcatttga
     4261 acagacagag tatatagc
//
LOCUS       DAOSHR010000170         4204 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_19070_length_4204_cov_5.537961, whole genome shotgun sequence.
ACCESSION   DAOSHR010000170 DAOSHR010000000
VERSION     DAOSHR010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 4204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4204
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19070_length_4204_cov_5.537961"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(114..1025)
                     /locus_tag="PLU28_08745"
     CDS             complement(114..1025)
                     /locus_tag="PLU28_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG35184.1"
                     /translation="MLAINVSGLTKKYKKFTAVDNISFSVEQGRIYGLLGPNGAGKST
                     IMKVLSTLISPCAGHVEIMGYSLEKDKEKIKSIISLVSDYTVLEDDLTPYENLKMFAE
                     ISVSMNSQVEGRISSLIEDFGLSMYKDKLTKNLSSGNKQKLNIARALIKKPQILLLDE
                     PTNAIDVETSRFIREYIVRENLESNTTVVISSHYIWEVEQIASEIGVIVNGKLAVNKK
                     SSEIFSDYKQFLRIFELAFPKEKFSEVSGFLDVSSGVSNFRQTGHDKLIVQTDGSEVA
                     FPDFVSVTEKELSLEDIYSHIVKKGAV"
     gene            complement(1035..1487)
                     /gene="smpB"
                     /locus_tag="PLU28_08750"
     CDS             complement(1035..1487)
                     /gene="smpB"
                     /locus_tag="PLU28_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOG35185.1"
                     /translation="MKIVANNKKAFHEYSMSDFMEAGIELAGSEVKSVRNGKVSFKDS
                     FCKVENYEIFLYNLHISPYENASIFNHDPERPRRLLLHKKEILRLNERVRKEGFTIIP
                     TKIYFNESGLIKVEIAIAKGKKVFDKREDLAKKDLERRMRKTEKYENI"
     gene            complement(1484..2566)
                     /gene="disA"
                     /locus_tag="PLU28_08755"
     CDS             complement(1484..2566)
                     /gene="disA"
                     /locus_tag="PLU28_08755"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:NF010009.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning diadenylate cyclase DisA"
                     /protein_id="HOG35186.1"
                     /translation="MKEMLPELLTMMAPGKPLRKGLDRIMDANLGVLMFFVEDAQKYI
                     DTNVIQLGFKIDVDFEPEKLYELAKMDGAIVLSKDGSRILYANVQLNTDSSIPSSETG
                     MRHRTAHKLAIQSQKLVIAVSKRRSTISAFYGKEKYELLSEAVLFSRLNQEITIAQRY
                     KQNFYELIEVLNSEESSNTVSLYDVSEIISKGLLTVRITQRSEIYLKEMGDLAGSAYL
                     EHREILSTVPKVLGAIIIDYTASVPEFDSCEEALSMFDSIKTENLLDLFLIAQRLGYK
                     KDSDEGLKETILTPRAFRLLYSTKLPQTSIKTIIGKYENLTNLLNVSLDELSAIGGIG
                     KRKARIITSALMRKKMQNEFEGGSYQ"
     gene            complement(2563..3921)
                     /gene="radA"
                     /locus_tag="PLU28_08760"
     CDS             complement(2563..3921)
                     /gene="radA"
                     /locus_tag="PLU28_08760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HOG35187.1"
                     /translation="MKKKEGKTYFICDECGYESQKWFAKCPECNVIGSAVEYTADSLY
                     ENGKASSDFHEVSVENLNSVTQIVPRIAVGMNELENILSGGLVKGGIYLVSGEPGIGK
                     STLVTQIFRNTGFPSIYISGEESKDQVFLRFKRLGISGENISIIFDTHLESILKAIES
                     KKLSPQLIVVDSIQTMKTDSFDSIAGSMIQVRECARILTEFGKRHGITVLLIGHVNKE
                     GNIAGPKVLEHIVDCVLQFELEVTTATRCLRVTKNRFGPTDEIAIFEMTQNGLIPIEN
                     IGNFFISDYSNQSGNTLSILQQGSKLIPIEVQALVSKPVYGQPRRITSGVALDRVLMI
                     TAVLSKKLKLPLESKDIFVNTSGGFRAFDNSTDLAIAVSLLSSLFDIKPSQSTVAIGE
                     IGLDGVVRPVGLNEKRAEFAVKLGIESILVPQKCASKNKKIQIIRDVRDILMMFNQEE
                     MK"
     gene            complement(3918..>4204)
                     /locus_tag="PLU28_08765"
     CDS             complement(3918..>4204)
                     /locus_tag="PLU28_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpC"
                     /protein_id="HOG35188.1"
                     /translation="IQMKELTKRLKQRDFEIKIKDQALSFLLEKGYDPIFGARPLKRA
                     IQRLLEDPLSEEILKGRFPPDSKISIELNKTSDALIFKKIARRVKKVPNA"
BASE COUNT         1187 a          937 c          674 g         1406 t
ORIGIN      
        1 tctggggacg ttataactgt tccgttgatt ttaacttttt ttcctgaaat tgctttttta
       61 cactcactcc tgcttatcga caaagaatcg catatatact tatcaagtct caatcatacg
      121 gctccttttt tcactatatg cgagtatata tcttccaatg aaagttcttt ttccgtaaca
      181 gatacaaaat ccggaaaagc aacttcagat ccgtcagtct gaacaattaa tttatcatgt
      241 cctgtctgcc tgaaattcga aacacccgaa ctaacatcta aaaaaccaga tacttcagaa
      301 aatttttctt tcggaaacgc gagttcaaaa attctgagaa attgtttata atctgaaaat
      361 atttccgatg actttttgtt gactgcgagt tttccgttta ctatgactcc tatttcagaa
      421 gctatctgtt caacctccca tatatagtgg gaagaaatta caaccgtagt gttagattca
      481 agattttccc ttacaatata ctcgcgtata aaccgtgaag tctcaacatc aatagcattt
      541 gtaggttcat ccaaaagaag tatctggggc ttttttatga gagcacgtgc aatattgagc
      601 ttttgcttat taccggatga aagatttttt gtaagcttat ccttgtacat gcttagccca
      661 aaatcctcta taagagaact gattctccct tcaacctgtg aattcataga cacagatatt
      721 tcggcaaaca tcttaagatt ttcataaggt gtgaggtcat cctcaagcac cgtatagtca
      781 gataccagac ttattattga cttgatcttc tccttatcct tttcaagaga atagcccatt
      841 atttcgacat gacctgcaca aggcgatatc aaagttgaca agactttcat tatagtggat
      901 ttccctgcac cattaggacc caaaagtcca tatatcctgc cctgctcaac actaaaagat
      961 atattgtcaa ctgcagtaaa tttcttatat ttttttgtga gaccagacac atttatagct
     1021 agcataagca cacctcatat attttcatac ttctcagtct tcctcattct tctttcaaga
     1081 tctttttttg caagatcttc tcttttgtca aaaacctttt ttccttttgc tatagctatt
     1141 tcaactttta taagaccgct ctcattaaaa taaatcttgg tcgggattat agtaaatccc
     1201 tccttgcgca ccctttcatt aagcctaagg atctcttttt tatgtagaag aagtcttctg
     1261 ggtctttcag gatcatgatt aaatatagaa gcattttcat atggacttat gtgcaggtta
     1321 tataaaaaaa tctcatagtt ctcaactttg cagaaactgt ccttgaaact taccttcccg
     1381 ttccgtacag acttgacttc tgatccggca agttctattc ctgcctccat aaaatctgac
     1441 atagaatact catgaaatgc tttcttgtta ttggcaacta ttttcattga tagctccctc
     1501 cttcgaactc attctgcatc ttcttgcgca taagtgcgct tgtaattatt ctggcctttc
     1561 tcttaccaat gcctccaatc gcagaaagct catcaagaga tacatttaaa agatttgtaa
     1621 gattctcata ttttccgatt atcgttttga ttgaagtctg cggtagcttc gttgaataaa
     1681 gaagtctgaa agcccttgga gtaagtatag tttctttaag accttcatct gagtcttttt
     1741 tatatcccag cctttgagct atcaaaaaaa gatcaagcag attttccgtc ttgattgaat
     1801 caaacatgga taaagcttct tcgcagctgt caaactcagg aacactggca gtataatcta
     1861 ttattatagc acccaggact ttaggcactg tagaaaggat ctccctgtgc tcaagatacg
     1921 cacttccggc aaggtcgccc atctctttaa gataaatctc tgatctttga gttatgcgaa
     1981 ccgttaagag acctttggat ataatctcgg aaacatcgta aagactgact gtattgctgc
     2041 tttcttcaga attaagaacc tcgataagct cataaaaatt ttgcttgtat ctttgggcaa
     2101 tggttatctc ctggtttaac ctgctgaaaa gaaccgcctc agaaagcagc tcatactttt
     2161 ctttgccata aaatgcagag atggtgcttc ttctctttga gactgcaatg acaagttttt
     2221 gagactgtat tgcaagctta tgggctgtcc tgtgacgcat tccagtttca gagctcggaa
     2281 tactgctgtc cgtgttaagc tgaacattgg cataaagtat cctgctccca tccttgctga
     2341 gcactattgc cccatccatt ttggccagtt cataaagttt ttccggttca aaatcaacat
     2401 ctatcttaaa tcctaactga attacatttg tatcgatata cttctgggca tcctccacaa
     2461 aaaacatcaa tactcccaga ttggcatcca ttatcctgtc aagtcccttt ctcaaaggtt
     2521 tccctggcgc catcattgta agaagctcgg gaagcatctc cttcatttca tctcctcctg
     2581 attaaacatc ataagaatgt ctctgacatc tcttatgatc tgtatttttt tatttttgga
     2641 tgcacatttt tgaggcacaa gaatactttc tattccaagc ttcactgcaa actccgccct
     2701 tttttcattc aacccgacag gcctgacaac tccgtccagt cctatttcac ctattgccac
     2761 ggtgctttga gaaggcttta tatcaaaaag agaagataaa agtgatacgg ctatagcaag
     2821 atccgtagag ttgtcaaaag ctctgaaacc tccagaagta ttaacaaaaa tatcttttga
     2881 ttccaacgga agcttcagtt tctttgacag cactgccgtt atcataagaa ctctgtccag
     2941 cgctacaccg gatgtaatac gccttggctg cccgtatacc ggtttactta cgagagcctg
     3001 cacctctatg ggtataagct tactgccctg ctgtaaaata gagagagtat ttccggactg
     3061 attagagtaa tccgaaataa aaaaattacc aatgttttca ataggaatca atccattctg
     3121 agtcatttca aatatcgcaa tctcatcagt tggtccaaac ctgtttttag ttaccctcag
     3181 acaccttgtc gccgttgtta cttcaagctc aaactgaagc acgcaatcaa caatatgttc
     3241 tagaactttt ggtccggcta tgtttccctc tttattaacg tgtccaataa gtaatactgt
     3301 tattccatgc cttttcccga attcggtgag aattcttgca cattctcgca cctgtatcat
     3361 gctacctgct attgaatcaa acgaatcggt tttcatagtt tggattgaat ccaccactat
     3421 aagctgagga gaaagttttt ttgattcaat agccttcaaa atgctttcta aatgggtatc
     3481 aaatattata gatatatttt cgccagaaat accgagccgt ttaaatctta gaaaaacctg
     3541 atccttcgat tcttcacccg agatataaat agatggaaaa cctgtatttc taaaaatctg
     3601 agtaacaaga gtgctctttc ctatccctgg ttctccactc acaagataga ttccgccctt
     3661 gacaaggcct ccgctcagaa tgttctccag ttcattcata cctacagcta ttcttggcac
     3721 tatttgagta acgctgttta aattttcaac gctgacttca tgaaaatccg atgaagcttt
     3781 tccattttca taaagactat ccgcggtata ctctaccgcg gatcctatta cattacactc
     3841 tggacatttg gcgaaccact tttgtgattc ataaccgcat tcatcacata taaagtacgt
     3901 tttgccttcc ttttttttca agcgttaggc accttcttga ctctccttgc aattttctta
     3961 aagatcaacg catcagaggt cttgtttaat tcaattgaaa ttttggaatc aggcggaaaa
     4021 cggcctttaa gaatttcttc agataacgga tcttccaaaa gtctttgtat tgcacgcttt
     4081 aatggcctag ctccgaatat tggatcgtaa cctttttcaa gcaggaaact taatgcctga
     4141 tccttgatct ttatctcaaa atctctctgt ttaagccttt tggtaagctc tttcatctgt
     4201 atgt
//
LOCUS       DAOSHR010000171         4200 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_19097_length_4200_cov_3.895537, whole genome shotgun sequence.
ACCESSION   DAOSHR010000171 DAOSHR010000000
VERSION     DAOSHR010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 4200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4200
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19097_length_4200_cov_3.895537"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            156..1751
                     /locus_tag="PLU28_08770"
     CDS             156..1751
                     /locus_tag="PLU28_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35189.1"
                     /translation="MPILTVYDVTGIQKFIFSSSKLKENFGGSLLVARVMDELLVAAV
                     KQANSKAYTDWKNSPDFKMLTDHDADAEIVYIGGGNAYVAYKDKATSVRTTKAFSKSV
                     FEETYSLTVLSASVEADFEKDDYKIKMKELQKTMDKKKNEFIRTSPLKNISITKESSN
                     LKPVIKKEKDEFLDTESIIKRNEFMDNHDIYNDKFLDGKNYIFPKDLENMGQRKGENH
                     IAVVHIDGNNMGKSIEKALENIKTFRQAVPRMRQISCNIALEYKDAFDQLIKFIIDKI
                     ENNESLKNRLNLSKDNNSTFLPIRPVILNGDDTTFVCDARIAMCLTEKFLQIIDEKQI
                     SPLGTTSACGGIAIIGSHFPFDKAYELAEECCSSAKRTAKEYATAHNENVKSWFDFHI
                     VYGGLTSELQTIREKTYNVVDLPSIKDSKIKKYHLLNRPFIVTEKDHNNSFEKFAVTY
                     ERFFGKKTVWARSNLKELRNVMIQGKQATELYLKELKSRGKELPDTYQNIKNGFFEQD
                     TTPFFDILEASDYFIDISDGRRA"
     gene            1754..3826
                     /locus_tag="PLU28_08775"
     CDS             1754..3826
                     /locus_tag="PLU28_08775"
                     /inference="COORDINATES: protein motif:HMM:NF015728.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RAMP superfamily CRISPR-associated protein"
                     /protein_id="HOG35190.1"
                     /translation="MRIKLELISDLCTSSGDTVSGIIDTEICYDENGIPYIPAKRVKG
                     CLKEAALELKDALGNEFDEETFKKLFGKSGDDRSASLVISNGYIENYNNISKEIKMMK
                     ISDKKYYYQKQFIVENYTSLRTNTRVDRETLTAEEDTLRITRVIDRGNTFYFDINDLD
                     KQEKDFLEKVCKVFRRMGLARTRGLGEVKCSLDSASNTSGGISMVDQNKLNDQNIYTV
                     KFHFQNISDLVISAVGGEGTLDYIPGSTIMGYFANSYIQKKKMDKQKAYKDKNFNELF
                     ISGKVIFSNAYISDEIGNTYLPCPRTVVKQKDGEKVYNKASDNIEEQVRNMDATYISF
                     QPNGTHYKKQTEKAVHFHHQRPKDRSIGHATENEGVFFQYETIKEGQYFVSSITATGA
                     NLKKLLDTIPNNKTISVGKSRSSQYGNLKITKTEITNNTPLTLKNNQEYVLTLTSALI
                     NTNENNDYTSTVQQLKTELEPIIGKFEIMGTYLKFAETSVYNAVSKTSSVRYNTLDRS
                     TAIKIKMNQDKTINTDTLWAGLMNNMGFGQIKVLNANMGNIQLINYEPLPAANPQINF
                     TQSIYKTIQEKTVISHIKVIAASEASKKAKTVNSTSVNQIIQFVKISQTYQEIMDKIN
                     TIKITDKKNECLSLIKEPGKFKTEVFEKLPIYQQLDPALKTDINKFHMHYIKAYLMQI
                     KYELRKVS"
     gene            3829..>4200
                     /locus_tag="PLU28_08780"
     CDS             3829..>4200
                     /locus_tag="PLU28_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35191.1"
                     /translation="MDKIIKRIYIKALAVLESPAIITAGENLNTDKDINIDKKGNAVI
                     QGTALAGIFRQYLAGSCKIKDEPPELKELFGNDHSRTADSTLSNIYISDSYITRPKVG
                     KRDGVKLELFNKIAVDQSKYDY"
BASE COUNT         1739 a          832 c          703 g          926 t
ORIGIN      
        1 gagttcacat taataattaa aaagatacta aaataaaaaa tataacaata attaacttat
       61 caactgatac aataataaat agagatttta aaaaagaaat aaaaatgata taattatatt
      121 tgacgtataa caaaaaaatt tgaaaggaga acattatgcc tatacttaca gtttatgatg
      181 taacgggtat tcaaaagttt attttcagtt cttccaagct taaggagaat tttggaggtt
      241 ctctccttgt ggcaagagta atggacgaac ttcttgttgc tgctgtcaaa caagctaatt
      301 caaaagctta taccgactgg aagaacagcc ccgatttcaa gatgcttact gaccatgatg
      361 cagacgcaga aatagtttat ataggcggag gaaatgccta tgtggcttat aaagacaaag
      421 caacttctgt aagaaccacc aaagcatttt caaaaagtgt atttgaagaa acatactctc
      481 tcactgtttt aagcgcatcg gttgaagcgg actttgaaaa agatgactat aaaataaaaa
      541 tgaaagaact ccaaaaaact atggacaaaa aaaagaatga attcataaga acctctccat
      601 taaaaaatat atccataact aaagaatcat caaacctaaa accggtaata aaaaaagaaa
      661 aggatgagtt tttagataca gaaagcataa taaaaagaaa cgaattcatg gacaaccacg
      721 atatttataa cgataaattt cttgacggga aaaactacat attcccaaaa gatctggaaa
      781 atatgggaca aagaaaagga gaaaaccata tagcggttgt acacatagac ggcaataata
      841 tgggaaaatc aatagaaaaa gctcttgaaa atattaagac attccgccag gccgttccca
      901 ggatgagaca aatatcttgt aatatagcgc ttgagtacaa agatgcgttc gaccaactca
      961 taaaattcat aatagacaaa atagaaaata acgaatctct caaaaataga cttaaccttt
     1021 caaaagataa taacagcaca tttcttccca taagacccgt aatactcaat ggagatgaca
     1081 caacattcgt atgcgacgcc agaatcgcca tgtgtctcac agaaaagttc ctccagataa
     1141 tcgatgaaaa acaaatctct ccattaggga caacttcagc ttgcgggggc atagccataa
     1201 taggatcaca cttcccgttt gacaaagcgt atgagcttgc cgaagaatgc tgctcatctg
     1261 caaaaagaac tgccaaagag tatgccacag cacacaatga aaacgtaaaa agctggtttg
     1321 atttccatat agtttacggc ggacttacat cagaactgca gaccataaga gaaaaaacct
     1381 acaatgttgt tgatctcccg tctataaaag actcaaaaat aaaaaagtac catctcctca
     1441 acagaccatt tattgtaacc gaaaaagacc ataacaactc atttgaaaag tttgccgtaa
     1501 cctatgaacg ctttttcggt aaaaaaacag tttgggcacg atcaaatctt aaagaactga
     1561 ggaatgtcat gatacaggga aaacaagcaa ccgaactata tctgaaagaa ctgaaatcaa
     1621 gaggaaaaga acttccggat acatatcaga atataaaaaa cggcttcttt gaacaagata
     1681 ctacaccatt ttttgatatt cttgaagctt cagattactt catagatatt tcagatggaa
     1741 ggagagcata aaaatgcgaa taaaacttga actaatatca gatctatgta caagctcggg
     1801 agacacagta tccgggataa tagacacaga aatatgctac gatgaaaacg gtataccata
     1861 tatacccgcc aaaagagtaa aaggctgcct gaaagaagct gctctggaac ttaaagatgc
     1921 tctgggaaat gaatttgatg aagaaacctt caaaaaacta ttcggaaaat ccggagacga
     1981 cagatcagca tcacttgtaa tatctaacgg gtacattgag aactacaaca atataagcaa
     2041 agaaataaaa atgatgaaaa tcagtgacaa aaaatactac tatcaaaaac agttcatagt
     2101 tgagaactac acaagtctga gaaccaacac cagagtagac agagaaacgc tgaccgcaga
     2161 agaagatacc ctcagaataa caagagtaat cgatagagga aataccttct actttgacat
     2221 caatgacctt gataaacaag aaaaagactt ccttgaaaaa gtatgcaaag ttttcagaag
     2281 gatgggactt gcaagaacca gaggtctggg agaagtcaaa tgctctctcg acagcgcaag
     2341 caacacttcc ggcggaatca gcatggtaga tcaaaacaaa ctcaacgacc agaacatata
     2401 caccgtaaag ttccattttc aaaatatatc agatcttgtt atttcagcag tcggaggaga
     2461 gggaacactt gactacattc ccggctccac tataatggga tactttgcca acagctacat
     2521 acagaagaaa aaaatggaca aacaaaaagc atacaaagac aagaacttta acgaactttt
     2581 catctcagga aaggtaatat tctcaaacgc ttacatatcc gatgaaatag gcaacaccta
     2641 tcttccgtgc cccagaaccg ttgtaaaaca aaaagacgga gaaaaagtat acaataaagc
     2701 ctcagacaac atagaagaac aggtaagaaa catggatgca acatacatat cctttcagcc
     2761 caacgggaca cactacaaaa aacaaacaga aaaagctgtt cactttcacc atcaaagacc
     2821 gaaagacaga agcataggcc atgcaacaga aaacgaaggc gtgtttttcc agtatgaaac
     2881 cataaaagaa ggacagtact ttgtttcaag cataacagca acaggcgcaa accttaaaaa
     2941 actgcttgat actattccca acaacaaaac catatccgtt ggaaaatccc gttcatcaca
     3001 gtacgggaac ctaaaaataa ccaaaactga aataaccaac aataccccac tgacccttaa
     3061 aaacaaccaa gaatacgttc ttacacttac ctccgctctt ataaacacaa acgaaaacaa
     3121 cgattacaca tcaactgtac aacagctaaa aacagaactg gaaccgatca taggaaagtt
     3181 cgaaataatg ggcacatacc ttaagtttgc tgaaacatca gtatacaatg ccgtttcaaa
     3241 aacaagctcc gttagataca ataccctgga cagatcaaca gccataaaaa taaagatgaa
     3301 ccaggataaa accatcaata ccgatacact ctgggcagga ctgatgaata atatgggatt
     3361 tggacagata aaagtactga atgcaaacat gggcaacata caactaatca actatgaacc
     3421 tttacctgca gcaaatccgc agattaattt cacccaaagc atctacaaaa ccatacagga
     3481 aaaaacggtt atcagccaca taaaagtcat agctgcaagt gaagcatcca aaaaagccaa
     3541 gacagtcaac tccacttcag ttaaccagat catacagttc gtaaaaatat cccaaaccta
     3601 ccaagagata atggataaaa taaacaccat aaagattaca gataagaaaa acgagtgcct
     3661 aagtcttata aaagaacccg gaaagtttaa aaccgaagta tttgaaaaac taccaatata
     3721 ccagcagctt gatccggctc ttaagacaga tataaacaag tttcacatgc actacataaa
     3781 agcatacctt atgcaaataa agtatgaact caggaaggtg agctgaaaat ggacaaaata
     3841 ataaaaagaa tatacataaa agctttagct gttttagaat ctcctgccat aataaccgca
     3901 ggcgaaaacc taaacacaga taaagatata aacatagata aaaaaggaaa cgcagtaata
     3961 caagggacag ctcttgcagg catattcaga cagtaccttg ccggatcatg caagataaaa
     4021 gatgaacctc ccgaattaaa agagcttttc ggtaatgacc acagtcggac cgccgactct
     4081 actctcagca acatatacat atcagactca tatataacca gacccaaagt cggaaaacgt
     4141 gacggcgtaa aacttgaact ttttaataaa atagccgttg accaatccaa gtacgactac
//
LOCUS       DAOSHR010000172         4153 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_19417_length_4153_cov_4.991947, whole genome shotgun sequence.
ACCESSION   DAOSHR010000172 DAOSHR010000000
VERSION     DAOSHR010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 4153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4153
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19417_length_4153_cov_4.991947"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(182..1204)
                     /locus_tag="PLU28_08785"
     CDS             complement(182..1204)
                     /locus_tag="PLU28_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOG35192.1"
                     /translation="MSDKITIKDIAKKSGVSISTVSRVINKTYPVRKEVAEKVQQTIQ
                     ELNYTPDLMAKSLKTGKTSTIGLIIPDISNPFFASLVKGAESYLSKKGYSLIICNSNQ
                     DMKEETRLINILLSKKIDGLLFTGSANHNKFLQEKVIDMLPVVFMDRAFVKTNGSYVL
                     SDSYNGMLDLLNYMYGANHRSYIYISGNKDTLSAGERTKAFSDFVKEKKIEKYRIYQA
                     QYDYESGYKAIKAMDCGKGYDAVICGNDLIAYGVIEALKNSGVNVPYNISVTGYDDIM
                     FSRMFSPSLTTVRQPIYDIGSKSAEKLLKIIENNKKIKNGIILKNELVIRESTMIRSD
                     CSRQKD"
     gene            complement(1382..2563)
                     /locus_tag="PLU28_08790"
     CDS             complement(1382..2563)
                     /locus_tag="PLU28_08790"
                     /inference="COORDINATES: protein motif:HMM:NF024601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA-like domain-containing protein"
                     /protein_id="HOG35193.1"
                     /translation="MDDILQKAWIELSKESVFYSYLKMYFDSLATDAVRTIKVSITPN
                     GNFRITYNPYKLQNKGLAFTKALLKHEIYHIIFGHFFIKIKNKREKGLWNICMDAAVN
                     QYIPELDALSMPMDALLKEGCASDNENLFVAPPMNYINKTAEEYFKWAVNFMEDEKMA
                     DLEEVEDKRSETDSHDFNSEISQEMAVDIVSEVISQAYDKSAGDIPSGIEFAVQLMIK
                     KPKVSWANVLRRFFGSSTVVDRYRSILKPNRRYEDQPGWQSEYGPKIAVILDTSGSII
                     EEEYNSFFSEIDSIAKITGGKVFLIQSDQDVRSVIQYSRGSWKDIVLKGKGSTDMQPA
                     VDYVQSVIRPEGIVVFTDGYVDVPVIKRRVMFVLSQKNNPEFYNESVEIYGKENVCII
                     Q"
     gene            complement(2580..3680)
                     /locus_tag="PLU28_08795"
     CDS             complement(2580..3680)
                     /locus_tag="PLU28_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOG35194.1"
                     /translation="MRPKDIKYLSEKVMSSGEIPLLWGHFGVGKTDIAKEIAQQTGRE
                     LIILVISQMEPGDLIGMPSRYKSSGMDSDRTVFLKPDWWPDNENTIIMIDEVNRAHRS
                     IRNAVMQLLLDRRIHNHILPPGTWIMAAANPPDEEYDQVDLITDPAFMSRFFHFDISP
                     DISDWTQWAKENKLADPVINFVNDYPEYLSSDLNVSMRLNLRPSPRSWYKLAKVFENL
                     SDQEIKDYGYIISASIIGSEAARAFTNHLNNKKNLPTAQDILKEGKFIQRLSSLNQDE
                     KITLVLRINKYIENISETDMIKIIDISEHQTYAKNIKLLSEYVPKDSIFAILRNLENL
                     IQKSSGLKRAFYDKLFEAVAIELNDESWFQNL"
     gene            3848..>4153
                     /locus_tag="PLU28_08800"
     CDS             3848..>4153
                     /locus_tag="PLU28_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35195.1"
                     /translation="MKKSLLLICFLVFPLILSLGYKTDIYLSKGDYINISDMDTPSYM
                     PPGSIYADRNFFFRASSSQATYTLFTAQDSWNIFINDPSRDATVLFYDFDTQQKNLSN
                     "
BASE COUNT         1141 a          834 c          685 g         1493 t
ORIGIN      
        1 agatcaagtg catagctcaa tgtaaaaaca gaaaacagaa ctgcaataac agctataaat
       61 ctctttaaaa acatgtttgc ccctccttta ttgatagaaa acgttttcat aagtgattaa
      121 aataaatctt ttaaaatgca agttatacag gtaacgattg atatttttaa aaaaatgatt
      181 actaatcttt ttgtctgctg cagtcacttc taatcatcgt gctttcacgt attactagtt
      241 catttttcag gattataccg tttttaatct ttttattgtt ttcgattatt ttcaacagtt
      301 tttctgccga tttacttcct atatcgtaga taggctgcct taccgttgta agggaaggag
      361 aaaacattct gctgaacatg atgtcatcat atcccgtgac acttatattg tatggcacgt
      421 tcacgccact gtttttaagt gcctctatta cgccataggc tataaggtca tttccgcata
      481 taactgcatc atatccttta ccgcagtcca ttgcttttat agctttataa ccgctttcat
      541 aatcatactg agcctggtat atgcggtact tttctatctt tttttctttt acaaagtcag
      601 aaaaggcttt ggttctctcc ccggcactca gagtatcttt gttgccgctt atgtatatgt
      661 atgaacggtg atttgctcca tacatatagt tcaagaggtc aagcatgcca ttgtaactgt
      721 cagaaagaac ataagatccg tttgttttca caaaagccct atccataaat acaacaggaa
      781 gcatgtcaat tactttttcc tgtagaaact tattgtgatt ggcacttccg gtaaacaaaa
      841 ggccatcaat tttttttgaa agcaaaatat ttatgagtct tgtttcttct ttcatatcct
      901 gatttgaatt acagataata agtgagtaac cttttttgct gagatacgac tcggcacctt
      961 ttacaagaga agcaaaaaaa gggtttgata tatccggtat tataagcccg attgttgatg
     1021 tcttacctgt tttgagactt tttgccatga gatctggagt atagttaagc tcctgtatgg
     1081 tctgctgaac tttttcagcc acttctttcc ttaccggata cgttttgttt ataactcttg
     1141 aaaccgtaga tattgatact cctgattttt ttgcgatgtc tttgattgta attttgtccg
     1201 acatgtctcc acctttctga aaacgtttgc gaaaacgttt tctaaattat tgtaatcatt
     1261 tttacaaatt aaagcaatcc tgattttcat ggtattaaca aagaatattc ttttttatgt
     1321 gtagttttct cttgttttct tgaaaaaccg aaaataaata cggccgcaga ttcggccgta
     1381 tttattgaat tatgcacaca ttttcttttc cgtatatttc aacagattca ttataaaatt
     1441 ccggattgtt cttttgggag agaacaaaca taacccgcct ttttatcaca ggtacgtcca
     1501 catatccatc ggtaaatact actattcctt cgggtcttat cacgctttgg acatagtcga
     1561 ccgcaggttg catatctgta gaacctttcc ccttaagaac tatatctttc cagcttcctc
     1621 tgctgtactg gataaccgaa cgcacatcct gatcagactg tatcaaaaaa acctttcctc
     1681 cggttatttt ggctatagaa tcaatttccg aaaagaagct gttgtactct tcttcaatta
     1741 tagaaccgct tgtatcaagt attacggcta ttttcggacc atattccgac tgccatccag
     1801 gctgatcttc ataccgtctg ttgggtttta atatgcttct gtatctgtcc accacggtag
     1861 agcttccgaa aaatctgcgt aaaacatttg cccacgatac tttgggtttt tttatcataa
     1921 gctgcaccgc aaactctatt ccgctgggaa tatcgccggc tgatttatcg tatgcctggg
     1981 atattacttc gcttactata tcaacagcca tttcctgaga aatctccgag ttaaagtcgt
     2041 gtgaatctgt ctcgctccgt ttatcctcca cctcttccaa atcggccatc ttttcatctt
     2101 ccataaaatt tactgcccat ttaaaatact cttctgcagt cttgttgata taattcatag
     2161 gcggtgctac aaaaagattt tcattatctg atgcgcaacc ttcttttaat aatgcatcca
     2221 taggcatgga aagtgcatcc agctctggta tatactgatt aacagctgca tccatacaga
     2281 tattccataa gcctttctcc cgtttgtttt ttatttttat aaaaaaatgc ccgaatataa
     2341 tgtggtatat ttcgtgtttt aacagtgctt ttgtaaaagc aagccctttg ttttggagtt
     2401 tatagggatt ataggttatt ctgaaattac cgtttggcgt tatgcttacc tttatggttc
     2461 tgacagcatc agtagcaaga ctgtcaaaat acatcttaag gtacgagtaa aaaacactct
     2521 cttttgaaag ctctatccat gctttctgta atatatcgtc cattttccca ccgctttatt
     2581 tataagttct gaaaccagct ttcgtcgttg agctcaatgg ctacagcctc aaaaagttta
     2641 tcgtaaaatg ctctttttaa accagaactt ttttgaataa ggttttcaag gtttcttaaa
     2701 atagcaaaaa tagaatcttt gggaacatat tccgaaagaa gcttgatatt tttggcatat
     2761 gtctgatgtt cagaaatatc aattattttt atcatatcag tctcgcttat attttcaatg
     2821 tacttattta ttctgagaac aagggtgatt ttttcgtcct gattcaatga cgaaagcctt
     2881 tgtatgaact ttccttcttt aagtatatcc tgcgcagtag gaaggttctt tttgttgtta
     2941 aggtggttgg taaatgctct ggccgcttct gacccgatta tcgaagcaga aataatatat
     3001 ccgtaatctt taatttcctg atcagaaaga ttttcaaaaa cctttgcaag tttgtaccag
     3061 cttctgggac taggtctcaa atttagtctc attgatacgt taagatctga cgataggtat
     3121 tcaggataat cgttcacaaa attaataacc ggatcagcaa gtttattttc tttggcccat
     3181 tgcgtccagt cgcttatgtc cggtgagatg tcaaaatgaa agaatcgtga cataaaagcc
     3241 ggatctgtta taaggtcaac ctggtcatac tcttcatccg gaggattagc cgccgccatt
     3301 atccatgtac caggtggcag tatgtgatta tggatacgcc tgtcaagcaa aagctgcatt
     3361 actgcatttc ttatcgaacg atgagctctg ttgacctcgt ctatcattat tattgtgttc
     3421 tcgttatcag gccaccagtc aggcttcaga aaaaccgtcc tgtccgaatc cattccagat
     3481 gatttgtatc tgcttggcat acctataaga tcgccaggtt ccatctgcga aatgaccaga
     3541 attatcagtt cccgtcctgt ctgctgggca atttctttgg ctatatcagt ttttcctact
     3601 ccgaaatgtc cccataaaag aggtatttcg ccgctgctca ttactttttc tgaaagatat
     3661 tttatatctt ttggtctcat ttctgcctcc tgatactttt tcttattttg gatattatct
     3721 ttattttaag tcttaaacac tgttgcattt tcattatatt atactacctg aagatatttt
     3781 tttctaaccg tatatttttt tcaccaaaat gatataattt tataaaaata ttattttgag
     3841 gtgtcatatg aaaaaatcct tattgttgat ctgttttttg gtttttccac tcatcctaag
     3901 tcttggatac aagacggata tctatctttc aaaaggagat tatataaata taagcgatat
     3961 ggacactcct tcatatatgc ctccgggctc aatctacgca gatcggaact tctttttccg
     4021 tgcgtcaagc tcgcaggcaa cctacactct ttttaccgca caggacagtt ggaatatctt
     4081 tataaatgat ccttcacggg atgcaactgt tcttttttat gattttgaca ctcagcaaaa
     4141 gaatctttca aat
//
LOCUS       DAOSHR010000173         4149 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_19447_length_4149_cov_6.009038, whole genome shotgun sequence.
ACCESSION   DAOSHR010000173 DAOSHR010000000
VERSION     DAOSHR010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 4149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4149
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19447_length_4149_cov_6.009038"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2075
                     /locus_tag="PLU28_08805"
     CDS             <1..2075
                     /locus_tag="PLU28_08805"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOG35196.1"
                     /translation="SMLKKILWYKRLLPIFVLIGLIPVLFFSYKISKNTYDSQLKNIQ
                     HYLQISNADKITSVKIFIQKFLDLSDMLGRESYFKTDLSEAESLEVKLKFKNIIETFS
                     ELNLLYFGAADGSFISYPQISLPDDYDHRERLWYEKAVRNPGTSIMTDPYEDIYNQQN
                     IISVARAVYNNEGKLLGVIGIDFVMNELIKLVESDKNYETDYSFAILHDGMTVLHTQA
                     DKIGAYTFYKKILEQSTDISGFITYGEKSNTRIAYYEKIPELKWIFYTVISEKEALEN
                     ARYVAGGMVLFAVLSVVVAFILELSFMFLVKKYTQNESMSAVNEELIAMNEEIEQSYN
                     DIHDLSTKLEVLLEVITSTINRIGESEKDYFTYIFNRLFDIVTEADYGLLCLVRDDDI
                     CFIASRGHSIEKLRSVGYTASDLIETEDVMIVDPQSAYKFFDEEKARKSLEVIEPCKQ
                     RMIITIYTDKKAVGNFILDIAKDSTKGFSQDSVKAMKVFSATASAFLTYNRYTDLKGR
                     MQKDIIMTMINILEVHDVYTKGHSNSVADIAVKIARAMDMNRQDVLMVYWAGLVHDIG
                     KILIPQAILNKPSKLTDAEYEEIKKHPEYGYIFLKKQGELKQIAEIVRYHHERFDGKG
                     YPKGLKGEEIPLFSRIISVADAYDAIISLRPYRAPLSAEQAREEIFKNSGTQFDPKIV
                     SVFFGLKL"
     gene            2103..2987
                     /locus_tag="PLU28_08810"
     CDS             2103..2987
                     /locus_tag="PLU28_08810"
                     /inference="COORDINATES: protein motif:HMM:NF025858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4438 domain-containing protein"
                     /protein_id="HOG35197.1"
                     /translation="MLKSNKEKVVMVSVQGKIHHPTGGNYRVSHEGFPMMLPATGGIS
                     YNVKVGDCAFGLSGDHIEPGVSIRNEDKNESDALMMLACIGNYARVVSGDAKGAVGYV
                     TGTHGGIEHVLVDFDRDDLEKMAVNDQILIKAYGQGLKLLDYPDIAVMNIDPVLFDKI
                     EIKEKESKIYVPVTAKVPAYLMGSGIGSLSAYSGDYDIMTADRKEVERLGLDKLRFGD
                     LVLLEDCDNTFGRGYLKGAVSIGVVVHSDCVKMGHGPGVTTILSCKSSQIEGYIDANA
                     NISVLMKKKEVQPKKAKK"
     gene            3235..>4149
                     /locus_tag="PLU28_08815"
     CDS             3235..>4149
                     /locus_tag="PLU28_08815"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG35198.1"
                     /translation="MAEIIIDNITFFYETPYKMIFQDLNLRIDTMWKTGLIGRNGRGK
                     TTLLNIIQKKSDVQKGRIINNIDCVYFPYQNFNPDADTFSVIKNAVAPYELMEKEMEE
                     LLSKNDEKSILRYMEVLNGYQAAGGYEIDSEILKEAQSIGIGAEILNRPFCTLSGGEK
                     TRSLLISVFLKKGSYALIDEPTNHLDMKGREILGEYLKSKSGFLMISHDRYFLDLCVD
                     HIITINKSQVEVMQGNYSDWEYNFTNRLKMEEKQNEKLTREIRSLQGASSLYRQVSGS
                     KEKEKYKGSQSDTSMIDRGFVGAKSAKLA"
BASE COUNT         1381 a          672 c          923 g         1173 t
ORIGIN      
        1 aaagtatgct gaaaaaaata ctatggtata aaagactcct gcctatattt gttcttatag
       61 gtcttattcc tgtattgttt ttttcgtata aaatctcgaa aaatacatat gattcacagt
      121 tgaaaaatat tcagcactat ctgcagataa gcaatgctga taagattact tctgtcaaaa
      181 tctttattca gaagtttttg gatctcagcg atatgctggg cagggagagt tactttaaaa
      241 cagatctttc tgaggccgaa agccttgaag tgaagttaaa atttaagaat attattgaaa
      301 cattttcaga acttaatctt ttatattttg gagctgccga cggaagtttc atatcctatc
      361 cacagattag tctgcctgat gattatgatc acagggaaag attatggtat gaaaaggctg
      421 tccgtaaccc cggaacatct ataatgacag atccctatga ggatatatat aatcagcaaa
      481 atataatttc ggtggccaga gctgtataca acaatgaagg aaagcttttg ggagttatag
      541 ggatagactt tgtgatgaat gagctgataa agcttgtcga gtctgataaa aactatgaaa
      601 cggactactc gtttgctatt ttgcacgacg ggatgactgt acttcatacg caggctgata
      661 aaatcggagc atacactttt tacaaaaaga tacttgaaca atccacagat atatccggct
      721 ttataaccta cggtgagaaa agcaacacca gaattgctta ttatgaaaag attccggagc
      781 ttaaatggat tttttatact gtaataagtg aaaaggaagc tttggaaaat gccagatatg
      841 ttgcaggagg aatggtttta tttgctgtgc tttccgtagt cgttgctttt atactggagc
      901 tttctttcat gttccttgtc aagaagtata cccaaaatga aagtatgagt gctgttaatg
      961 aagaacttat agctatgaat gaagaaatag aacagtcgta caacgacatt catgatcttt
     1021 cgactaagct tgaggttctt cttgaagtaa taacaagtac gataaacagg attggcgaat
     1081 cggaaaaaga ttatttcaca tatattttca acaggctttt tgatatagtt acagaagccg
     1141 attacggtct tttatgtctg gtaagggatg acgatatatg ttttatagcc agcaggggac
     1201 atagtattga aaaacttcgg agtgtagggt ataccgcttc tgatcttatt gaaactgaag
     1261 atgtgatgat agttgatccc cagtcagcat acaagttctt tgatgaagaa aaagcgcgta
     1321 aatcccttga ggtgatagaa ccgtgcaaac agcgcatgat aataaccata tatacagata
     1381 agaaggctgt cggaaatttt atactcgata ttgccaaaga tagtacaaag ggtttttctc
     1441 aggactctgt taaagcaatg aaggtatttt ccgcaactgc gtcagcgttt cttacgtata
     1501 accgctatac agatcttaaa ggaagaatgc agaaagatat aatcatgacc atgataaata
     1561 tactggaggt tcatgatgtt tataccaaag gccattcaaa cagcgtagcc gatatagctg
     1621 taaagatagc cagggcaatg gacatgaaca gacaggacgt tttgatggtt tattgggcag
     1681 ggcttgtaca tgatataggt aagatactta taccgcaggc tattctgaat aagccttcca
     1741 agcttacaga tgcagagtat gaagaaataa aaaagcatcc tgagtacgga tatatatttc
     1801 ttaaaaaaca gggagagctt aagcagatcg cagagatcgt acgctatcat catgaacgtt
     1861 tcgacggaaa aggttaccct aaaggactta agggcgagga gattcctctt ttttccagga
     1921 taatatcggt tgctgatgcc tacgatgcca ttatcagtct aagaccatac agagcacctt
     1981 taagtgctga gcaggcgcgt gaagaaattt ttaaaaattc tggaactcag tttgatccta
     2041 aaattgtttc ggtgttcttc ggtctgaaat tataatattt tatatactgc ggaggattaa
     2101 ttatgttaaa atcaaacaaa gaaaaggttg taatggtgtc ggttcagggt aagatacatc
     2161 atcccacagg cggaaactac agggtgagtc atgaaggttt tccaatgatg cttcccgcaa
     2221 caggaggcat atcatataat gtgaaggttg gagactgtgc ttttggtctg agcggcgacc
     2281 atattgagcc gggagtgtcc ataagaaatg aagataaaaa tgaatctgat gcccttatga
     2341 tgcttgcatg tatcggaaac tatgcaagag ttgtaagcgg tgatgccaaa ggtgctgttg
     2401 gttatgtaac aggtactcat ggcggaatag aacatgtact tgttgatttt gaccgtgacg
     2461 atctggaaaa aatggctgtc aatgaccaaa tacttataaa agcatacgga cagggtctca
     2521 agcttctgga ctatccggat atagctgtga tgaacataga tccggtactt tttgataaga
     2581 tagaaataaa agaaaaggag tccaagatat atgtacctgt cacagcaaag gttcctgcat
     2641 atcttatggg atcaggtatc gggtctttgt cagcatacag cggagattac gatataatga
     2701 ccgctgacag gaaagaggtg gaaagacttg gactggataa actccgattt ggtgatttgg
     2761 ttcttctgga ggattgcgat aatacattcg gaagaggata ccttaaggga gccgtatcaa
     2821 tcggagtggt ggttcattca gactgcgtga agatgggtca cgggccggga gttaccacta
     2881 tactgtcatg caaatcctct cagatagaag gatatataga tgccaatgcc aacatatctg
     2941 ttttaatgaa aaaaaaggaa gtacagccca aaaaagcaaa aaagtaacat aaaatcaatg
     3001 gttgtgtatt aaaaaagttc catatctttt ttataatata ttcatcatac taaaaatatt
     3061 aggagtgtta agatgaaaat ttcccaaacc gttatcagaa aaattaataa tacgtatcaa
     3121 caaggatagc ttaccggttt gtggaactgt ttgtcttaag gattatctat agataattac
     3181 aatggaaacc gcgggtttgc tgcctgcggt ttttttattt ttatggagga agatatggca
     3241 gaaataataa ttgataatat aacttttttt tatgaaacac cgtataaaat gatctttcag
     3301 gatctaaatc tcagaataga cactatgtgg aagaccggtc ttatcgggag aaatggacgg
     3361 ggaaagacga ctttgctaaa cataatacag aaaaaatcag atgttcaaaa gggtagaatt
     3421 atcaacaata ttgattgtgt ttactttcct tatcagaact ttaaccctga tgccgacacc
     3481 tttagcgtta taaagaatgc cgttgcccca tatgagctta tggaaaaaga gatggaggag
     3541 ctgctttcaa aaaacgatga aaagtcaata ctccggtaca tggaagttct gaacggctat
     3601 caggctgcag gaggatacga gattgattcg gaaattttaa aagaggcaca gagtattgga
     3661 atcggggcag aaattctcaa ccggcccttt tgtacattga gcggcggtga aaagacaaga
     3721 agcctgttga tttcagtttt tctaaaaaag ggctcatatg ctcttataga cgagccgacc
     3781 aatcatctgg acatgaaagg cagagagatt cttggcgagt atctgaaaag caaaagcggt
     3841 tttctgatga tatctcacga caggtacttc cttgatttgt gcgttgatca cataatcacc
     3901 ataaataagt ctcaggttga ggttatgcag ggaaactact ccgattggga gtataacttc
     3961 accaacaggc tgaagatgga agaaaagcag aatgaaaagc ttacccgtga gataagatct
     4021 ttgcaggggg catcatcact ttacagacag gtatccggct caaaagaaaa agaaaagtat
     4081 aaaggcagtc agtccgacac atcaatgata gacagagggt ttgtaggtgc taaatcagct
     4141 aaacttgcg
//
LOCUS       DAOSHR010000174         4149 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_19451_length_4149_cov_4.952125, whole genome shotgun sequence.
ACCESSION   DAOSHR010000174 DAOSHR010000000
VERSION     DAOSHR010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 4149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4149
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19451_length_4149_cov_4.952125"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="PLU28_08820"
     CDS             <1..329
                     /locus_tag="PLU28_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HOG35199.1"
                     /translation="DKMSPAVRMVESTQFEADVVLRDSNAIAYMGIGYVDSNVKVLKV
                     EGVMPSKTTVLDSTYIMSRPLYMFINATEGFPMTGLVKEYFSFATSKKGQELVEKAGF
                     IAAYGN"
     gene            411..1277
                     /gene="pstC"
                     /locus_tag="PLU28_08825"
     CDS             411..1277
                     /gene="pstC"
                     /locus_tag="PLU28_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HOG35200.1"
                     /translation="MNKREIKNNSKNVMVVCVACFSILILVMIFGFIIKESLPAISSE
                     GFKLFSGMLWYPTHSEPEYGLAAMFIGSLLITLLTAVIVLPLGYIIAFFMYGYAMPWE
                     KNIIKSSIDLLSGVPSVIIGSFLLVYVAPIFFKLGAWSRENILLGAVGLTILSLPYTA
                     SLMEEAMSSVDKSMKEGALALGATKFRAGFSVISKAAMPGLMNAVILTLNRIIGETMV
                     VLMASGGAAIIPMSLWDPVRPLTAAIASEMGEVELGSIHYSALFAAGLVLLTISFILT
                     LISKKISRRSSK"
     gene            1274..2107
                     /gene="pstA"
                     /locus_tag="PLU28_08830"
     CDS             1274..2107
                     /gene="pstA"
                     /locus_tag="PLU28_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HOG35201.1"
                     /translation="MKRDIAISIIFRVISYAAFLVVIAIFLTFILEGFMYFSPEFFTQ
                     FPQMGMTKGGIFPAIVGSVSVLMISLAVAIPLGVFTGVFLSEYAGKGFFSKIVDTSIT
                     ALSGVPSIVYGLFGLSIFCITLGFRTSILSGSLTLAIMTLPVISSSVREALQAVPREL
                     RESAYALGAKKSEVVFKVVLVAAKERIITAVLIGGGRVIGETAPILLTGAIFYSTMMP
                     KGILDPVMTLPSHIYYITMAYGQSAQWMAKGASAFLMIFILIIYSTAFYIRRKISNAH
                     K"
     gene            2094..2870
                     /gene="pstB"
                     /locus_tag="PLU28_08835"
     CDS             2094..2870
                     /gene="pstB"
                     /locus_tag="PLU28_08835"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HOG35202.1"
                     /translation="MPINDDKPSIVISVRNFNAYYSDKKALKEINLDFYKNKITAIIG
                     PSGCGKSTLLRSINRMNDEIAGFRTEGEILYNGKNVNDKKLDLTLYRKNVGMVFQKPT
                     PFPMSIYDNVSFGPRIHGIKNKSKIEDIVESSLKKAALWEEVKDELHKSALSLSGGQQ
                     QRLCIARALAVDPEVILLDEPTSALDPIATQKIEILLEELSQNYSIIIVTHNLSQAVR
                     ISDYMAYMFQGDLIEYDSTSKIIRSPKNELTEQYLTGRIG"
     gene            2883..3545
                     /gene="phoU"
                     /locus_tag="PLU28_08840"
     CDS             2883..3545
                     /gene="phoU"
                     /locus_tag="PLU28_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HOG35203.1"
                     /translation="MENTRHYDNEMLFLRAEVSKMLSLVLESFELAVKSLEDVDTFIA
                     DKVIAGDDTIDDLNRQIEETVYMIVARYSPQSKDLRYAMTMVKFANNLERIADLSCNI
                     AEMTKDIYEKNIKINLIKDIKRMFGICLQMIKDTYEAFGGKNIKLAIEVWRRDNDIDN
                     LEKIVNSSVIDGICNEQFDKRLAILYILLARDLERIADHSTNLCEEIIYIETGKDIDE
                     FI"
     gene            3565..>4149
                     /locus_tag="PLU28_08845"
     CDS             3565..>4149
                     /locus_tag="PLU28_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOG35204.1"
                     /translation="MASVLIVEDDEDIRDILKTYLTIEKIDVFEADSLNEMREKLKKF
                     AVDLILLDIMLADGDSIDELPNIRAKYPEIGVIIVSAKGTDRDKIFGIESGADDYVTK
                     PFNPREVVARVRALLKRTKKENDVLKFGSLEIYPNNYSVKYKTEVIELTGKEFEVLHL
                     LARNPDRIFSREEIIDKVWYDDDYITDRVVDVHIS"
BASE COUNT         1342 a          677 c          868 g         1262 t
ORIGIN      
        1 ctgataagat gtctccggca gtaagaatgg ttgaatcaac ccagtttgaa gcagatgtgg
       61 ttctcagaga ttccaatgct attgcatata tgggtattgg atatgtggat agcaatgtaa
      121 aggttcttaa agtagaagga gttatgcctt caaaaactac tgttcttgat tctacatata
      181 taatgtccag acccttgtac atgtttatca atgcaactga aggttttccg atgactggtc
      241 ttgttaaaga gtatttctcc tttgcaactt caaaaaaagg tcaggagtta gtggaaaaag
      301 ccggattcat agctgcctac ggaaactaac aatagattat aaactgcagc aggggatagt
      361 tttcccctgc ttatgcctat gatcatgaaa taagaggtgt ttaattttat atgaataaaa
      421 gagagataaa aaataattct aaaaatgtta tggtagtttg tgtagcttgt ttttcaattc
      481 tcatcctagt aatgattttt ggctttataa taaaggaatc actgcctgca ataagcagtg
      541 aaggttttaa gctctttagc ggaatgttat ggtatccaac tcattcagaa ccggagtatg
      601 gtcttgctgc aatgtttata ggttctttgc ttataacgct actcacagca gttattgttc
      661 ttccgctagg ctatataatt gcttttttta tgtatgggta tgccatgcct tgggaaaaaa
      721 atataataaa atcctcaata gatctccttt caggagttcc ttcagttatt ataggctctt
      781 ttttgcttgt atatgtcgca cctatatttt ttaaattggg agcatggtca agggaaaaca
      841 tccttcttgg ggcagtaggt cttaccatac tttcactgcc ttatacagct tctcttatgg
      901 aagaagctat gagctctgtt gacaaatcca tgaaagaagg agcactcgcc ttgggagcaa
      961 ccaagttcag ggcaggattt tcggttatct caaaagccgc tatgcctggt ttgatgaatg
     1021 cagttatcct aactttgaac agaataatag gagaaactat ggtagtgctt atggcaagcg
     1081 gaggtgcagc aataattcca atgtctttat gggatccagt ccgtcctctt acggcagcaa
     1141 ttgctagtga gatgggagaa gtagaactgg gaagtattca ttattcagct ctttttgccg
     1201 caggtcttgt ccttttaacc atctctttta ttctgacgtt aatatccaag aaaatatccc
     1261 ggaggagttc aaaatgaaaa gagacatagc aatttccatt atatttagag ttatcagtta
     1321 tgctgcattt ctagttgtaa tagcaatatt tcttaccttc atacttgaag gctttatgta
     1381 cttttctcct gaatttttta cccagtttcc acagatggga atgacaaagg gaggtatatt
     1441 tccggcaata gtcggttctg tatcagtact gatgataagt cttgcggtgg caattcccct
     1501 tggtgttttt acaggagtat ttctttcgga gtatgcagga aaaggttttt tttcaaagat
     1561 tgttgataca tcaataactg ctctttcagg agttccttca atagtgtacg gactgttcgg
     1621 tttatctatt ttttgcatta cgttagggtt cagaacctct atactttcag gaagcctaac
     1681 acttgcaata atgacactgc ctgttatctc atcatcggta cgcgaagctc ttcaagctgt
     1741 tcccagagaa ctgcgtgagt cagcctatgc attgggcgca aaaaagtctg aggtggtttt
     1801 taaggttgtg cttgttgcgg caaaagaacg tataataact gcagtattga taggaggcgg
     1861 tagggttata ggggaaacag cacctatact tcttacagga gcaatatttt actctacaat
     1921 gatgcctaaa gggatactcg atccagtaat gactttgccg tcacatatat actacattac
     1981 aatggcttat ggtcaatctg cacagtggat ggctaaaggc gcatcagcat ttcttatgat
     2041 ttttattctg attatatatt caacggcttt ctacataagg aggaagatta gcaatgccca
     2101 taaatgatga taaaccaagt atagtgatat cagtaaggaa cttcaatgct tattattctg
     2161 ataaaaaagc tcttaaagag ataaatcttg acttttacaa gaataaaatc acagctataa
     2221 ttgggccttc cggatgcgga aagtcgacac ttctgcgtag tataaacaga atgaacgatg
     2281 agattgccgg tttcagaacc gaaggagaaa ttttgtataa cggtaagaac gttaacgata
     2341 agaagctgga tcttacacta tacaggaaaa atgtcggaat ggttttccag aagcctacgc
     2401 cttttcctat gtctatatac gataatgtat cttttggtcc aagaatacac ggtattaaaa
     2461 ataaatctaa aattgaggat attgttgaaa gctcactcaa aaaagcagcg ctctgggaag
     2521 aggtaaagga tgagcttcac aagtcggcat tgtcactttc aggtgggcag cagcaaaggc
     2581 tgtgcattgc gagagctctt gcggtcgacc ctgaggtaat ccttctggat gaaccaacat
     2641 ctgcgcttga ccctatagcg acgcaaaaga tagaaatcct tctcgaagaa ttatcacaga
     2701 attatagtat aattatagtc acacataact tatcacaggc tgtaagaata tctgattata
     2761 tggcatatat gtttcagggt gaccttattg agtatgacag tacatccaag ataataagat
     2821 cgcccaaaaa cgaattgacc gaacagtatc ttacaggaag aataggttaa gcggaggttc
     2881 ttatggaaaa taccagacat tatgataatg aaatgctttt tttaagagct gaagtatcaa
     2941 aaatgctttc tttggttctg gagtcttttg agcttgctgt aaaatccctt gaagacgtcg
     3001 atacatttat agctgataaa gttatagctg gagacgatac cattgatgat cttaacaggc
     3061 agatagagga aactgtatac atgatagtgg caagatacag cccgcaatct aaagatctga
     3121 gatatgctat gacgatggtg aaatttgcaa acaatcttga gcgcattgct gatctttcgt
     3181 gtaatattgc tgaaatgaca aaagatattt atgaaaaaaa tatcaaaata aatcttatta
     3241 aagacataaa aaggatgttt ggaatatgtc ttcagatgat aaaggatacg tacgaagctt
     3301 tcggaggcaa gaatattaag cttgccattg aagtgtggcg aagagataac gatattgaca
     3361 atctcgaaaa aatagtaaac agcagtgtaa tagacggaat ctgcaacgaa cagttcgata
     3421 aacgtttagc tatactttat attcttcttg ccagagacct tgaaagaata gccgatcatt
     3481 ccacgaatct atgtgaagag ataatatata tagaaacagg taaagatata gacgaattta
     3541 tatgaatgaa aacaggaggc tcttatggca tcagttttaa tagttgagga tgatgaagat
     3601 ataagagata ttctcaaaac ctatcttaca atagaaaaaa tagatgtatt tgaagcagat
     3661 tctctaaatg aaatgagaga aaaattaaaa aaatttgctg tcgatcttat tcttctggat
     3721 ataatgcttg ctgacggtga ttcaattgac gaacttccaa atataagagc aaagtatcct
     3781 gaaataggtg taataatagt ttctgcgaaa ggaacagaca gagacaaaat attcggtata
     3841 gagtccggtg ccgatgacta tgtaaccaag ccatttaatc ccagagaagt tgttgcaaga
     3901 gtaagggcgc ttctgaagag aactaaaaaa gaaaatgatg ttcttaagtt cggaagcctt
     3961 gagatatacc cgaataatta ttctgtaaaa tataaaactg aagttataga acttacgggt
     4021 aaagagtttg aagttctaca tcttcttgca cgtaatccgg ataggatatt ctctagagaa
     4081 gagattatag ataaggtctg gtatgatgat gactatataa cagacagagt ggttgatgtg
     4141 catataagc
//
LOCUS       DAOSHR010000175         4129 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_19593_length_4129_cov_4.786451, whole genome shotgun sequence.
ACCESSION   DAOSHR010000175 DAOSHR010000000
VERSION     DAOSHR010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 4129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4129
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19593_length_4129_cov_4.786451"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..783)
                     /locus_tag="PLU28_08850"
     CDS             complement(<1..783)
                     /locus_tag="PLU28_08850"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HOG35205.1"
                     /translation="MKEKIRGSIIGAALGDALGAPIEKLSHSKIKEMYGKVTDLNVES
                     YKSKSKTHGKGYGRITDDTLLSIRLCEMYNEKIDHIDTYDVFTLIKKIFFQKMWVPEY
                     QKEIPLIERLFYPEKYIFLSNYLASRDPRSGGTGNMVNCGAAMYMTPVGLVNACDPLR
                     AYEEAINFGLSHQHSYGLEAAGVFAACVAAAMIPGIKMSEVASLAIDLAKDGTQLAIK
                     DIVKETVLMDKNEDNADKFHEIIKKYSPMGDNVERFEDQVGLP"
     gene            complement(793..2190)
                     /locus_tag="PLU28_08855"
     CDS             complement(793..2190)
                     /locus_tag="PLU28_08855"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOG35206.1"
                     /translation="MKLKALLVLCMIISAVVVFAKPTVIRLLEQDFLPSNPDDEQYVK
                     LIETEFLKATGIEIDIQLVPIPSGKYADKVNVLLASGDFPDIIWFRDNVDIPYADQGL
                     LMDLRALVSKSETLKKAMPSWDMARLNSYPYLLWITPINPKIAVVREDWMKELGISAP
                     KTVEDYYDMMKKLKTKAPNAITITGNLERLDSIFNAAFGTDQTFIKQKDGTFTYSRIT
                     QGEKEKLAFYNKLFKEGLLDPEFITVNWQSMEDKLYSSKVAMVVGGSGIVVDMYQQRM
                     YDSGNNTKLIALEPPVGISGKAGFPAVNVNRQSRGFAITTVSKVKDVAFKLLEFMATE
                     RGQFIDRLGVEGREYKVVNGKIEKTEKGLVWYPRFYENPNWNSPIELYGEVGKKSLEI
                     VNKYYVEDNYFEMPAKYSTSWDEMNNLYKEYSIKIVTGEYAIEKFDEFVTKWRKAGGD
                     TYIKLANEFFKKSGK"
     gene            complement(2214..>4129)
                     /locus_tag="PLU28_08860"
     CDS             complement(2214..>4129)
                     /locus_tag="PLU28_08860"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOG35207.1"
                     /translation="AVIAVLFSVFTLSYALDLKGTTESSSIDSKILGHAFKYKVYLPA
                     EYTSQKQFPVIYLLHGSNGNENSWDELLGTLDTLISCGEIPPFAAVVPCSGYSWWIDG
                     REKYESAFINELMPLVEKKYNFSQYRETRIISGFSMGGYGALRYALVYPELFSGAILL
                     SPALYDTLPPIDSSARETGNFGMPFDIVKWNTLNYPQALKKYRQQSSTVSLFIAAGDD
                     DWHNPEGFEFNIEQQAVRAYGILCKENKIPGELRILNGGHDSGVWKPAFSEGLKYLGS
                     IVSKEIKKLSSSPAIKTEVKIDKVWSFKAAGAINSSPAVGNDGTIYFGCDGKKIYALT
                     PDGNKKWEYATDDAVYATPAIDNDGTVYFACWDGFLYAMNPDGTLKWKNNTGCGDSSP
                     CIGVDGSIYIGSSDGKLICIDKNSTIKWQVQLGSIVTSSPITDTRMIFVASEDNKFFA
                     FSLDGQKIWQAELDSWITSSPSFYDENIVMAVSGSSLYVFEKSTGKVIKQIAVSGGRS
                     SAVTDKNGNIYIGTREDKKLYAFNKNFEKIWDFATGARVYSTPLTVSGGLIMFGSRDK
                     SFYCVDSFGNMIYELKTGGEVNSSPVMAEDGTLYIGSADSQLYAIRFTDASFQESLWP
                     MFRGNKRRNGSTK"
BASE COUNT         1118 a          946 c          690 g         1375 t
ORIGIN      
        1 cgggagtccg acctgatctt caaatctttc aacattatct cccatagggc tgtatttttt
       61 tatgatttca tggaacttat ctgcattatc ttcattttta tccattaata ctgtttcttt
      121 tacgatatct tttattgcaa gctgtgttcc gtcttttgcc agatctattg ctaacgaggc
      181 aacttcggac atttttattc cgggtatcat ggcagccgcc acacatgccg caaaaacacc
      241 tgcggcttca agaccatagc tgtgctgatg agacaaacca aaatttatgg cttcctcata
      301 tgctcttaac ggatcacatg catttacaag tcctacaggt gtcatataca ttgctgctcc
      361 acagttcacc atatttccgg ttccgcccga ccttggatcg cgtgatgcaa gatagttgct
      421 gagaaaaata tatttctccg ggtaaaaaag tctttctata agaggaatct ctttttgata
      481 ctcgggtacc cacatctttt ggaagaaaat ttttttgata agtgtgaaca catcgtacgt
      541 atcaatatga tcaatttttt cattatacat ctcgcatagc ctgatactca acaatgtatc
      601 gtctgttatt cttccgtaac cctttccgtg agtctttgat tttgacttgt agctttcaac
      661 attcagatct gttactttgc cgtacatttc ttttattttt gaatggctaa gcttctcaat
      721 gggcgccccg agagcatctc ccagggcagc accgattatt gaacctctta tcttctcttt
      781 cattattctt tcctacttac cggatttttt aaaaaattca ttagcaagct ttatatatgt
      841 atctccacct gcttttctcc atttggtgac aaactcgtca aatttctcta ttgcatactc
      901 cccggtaaca atctttatgg agtactcttt ataaagattg ttcatttcat cccagctagt
      961 cgaatacttt gcaggcattt caaaataatt atcttctaca taatatttat taactatttc
     1021 aagagatttt ttgccaactt cgccataaag ctctattggt gagttccagt taggattttc
     1081 atagaatcga ggataccata caagaccttt ttctgttttt tcaatctttc cgttaactac
     1141 cttgtactct ctgccttcaa ctcccagcct gtctataaac tgaccccttt cagtagccat
     1201 gaattcaaga agtttaaacg caacatcttt tacctttgag acggttgtta ttgcaaaacc
     1261 tcttgactgc ctgttaacgt tcacagccgg gaaacctgcc ttgccggaga ttccgaccgg
     1321 aggttccaag gctataagct ttgtattatt tcccgaatcg tacattctct gctgatacat
     1381 atccacaact attcccgatc ctcctacaac catcgctact tttgaagaat agagtttatc
     1441 ttccatactc tgccagttaa cggtgataaa ttccggatca agcagccctt ccttaaaaag
     1501 cttgttataa aaagccagct tttctttttc accctgagtt attctcgaat acgtgaatgt
     1561 tccgtctttt tgcttaataa aggtctgatc ggttccaaat gcagcattaa aaattgagtc
     1621 aagtctttca agatttcctg ttatagtgat tgcattaggt gctttagttt taagtttttt
     1681 catcatatca taataatctt ctactgtctt aggagcagaa attccaagtt ctttcatcca
     1741 atcttccctt acaacggcga tctttgggtt tataggagtt atccatagca gatacgggta
     1801 tgaatttaat cttgccatat cccatgaagg cattgctttt ttcagagttt cggattttga
     1861 taccagtgct ctgagatcca taagaagccc ctgatcggca tatggtatat caacattgtc
     1921 cctgaaccat attatatccg gaaaatcacc gcttgcaagc agaacgttga ctttgtcggc
     1981 atacttacca cttggaatcg gaaccagctg tatatcaatc tctataccgg ttgcttttaa
     2041 aaattccgtt tctataagct ttacatactg ctcatcatca ggattggagg gaagaaaatc
     2101 ctgctccaga agccttatga ctgtcggttt tgcaaaaacc acaactgctg aaatgatcat
     2161 acacagcacc aataacgctt ttaacttcat acatccacct ccgaaataga attttatttt
     2221 gtacttccgt ttcttctttt atttcctctg aacatcggcc ataaagactc ctgaaaagac
     2281 gcatctgtaa atcttatagc ataaagctgt gaatctgcag atcctatgta aagcgtcccg
     2341 tcttctgcca ttacaggtga tgaatttact tctccccccg ttttgagttc atagatcata
     2401 ttcccaaaag aatctacaca gtaaaaagac ttgtcccgtg aaccgaacat tatcaaccca
     2461 ccggaaactg tcagtggagt tgaataaact cttgctcctg ttgcaaaatc ccagattttt
     2521 tcaaaattct tattaaatgc atataatttt ttatcttcac gggttccaat atatatgtta
     2581 ccgtttttat cagttaccgc cgaggatctt ccgccactga ccgcaatctg cttgatgact
     2641 ttgcccgtgc tcttttcaaa gacatataat gaacttcctg atactgccat aacaatattt
     2701 tcatcataaa atgaaggcga tgaagtaatc caactgtcaa gctctgcttg ccatatcttt
     2761 tgtccatcca aggaaaatgc aaaaaatttg ttgtcttcac ttgccacaaa tatcatgcgt
     2821 gtgtctgtaa tcggagaaga agtaactatc gatccaagct gcacctgcca cttgattgtg
     2881 gagtttttat ctatacatat aagttttccg tcagaactgc ctatatatat gcttccgtcc
     2941 actccgatac aaggcgagga atctccgcat ccggtattat ttttccattt aagcgtacca
     3001 tccggattca tggcatacaa aaatccgtcc cagcaggcaa aatagactgt cccgtcatta
     3061 tctatggccg gtgttgcata tacagcatca tctgttgcat actcccattt tttgtttccg
     3121 tctggagtca gggcatatat tttcttgccg tcgcatccaa agtatatggt tccgtcattt
     3181 cctaccgctg gagaactgtt tatagcgcct gcggctttaa aactccatac cttgtctatt
     3241 tttacttctg tttttatggc cggagaagaa gaaagctttt taatttcttt tgaaacaata
     3301 cttcccagat actttaaccc ttctgaaaat gcaggcttcc atactccgct gtcatgtcct
     3361 ccattaagga ttctcaactc acctggtatt ttgttttcct tgcataaaat gccgtatgcc
     3421 cttactgcct gttgttcgat gttaaattca aagccttcag gattatgcca gtcatcatcg
     3481 cctgcggcta taaaaagact gacggtgcta ctttgctgtc tgtacttctt aagagcctgc
     3541 ggatagttga gagtgttcca cttaacaata tcaaaaggca tgccgaagtt gcctgtttcc
     3601 ctggcacttg aatctattgg cggtaaagtg tcataaagtg ctggacttaa aaggatggca
     3661 ccgctgaaaa gttccggata taccagagcg tatctcaaag caccatagcc ccccatggaa
     3721 aagcctgata ttatccttgt ttctctatac tgtgaaaagt tatacttttt ttctaccaaa
     3781 ggcataagtt catttataaa cgcactttca tacttttctc ttccgtcaat ccaccagcta
     3841 tagccgctgc atggaacaac agctgcaaac ggaggaattt ctccgcaaga aataagcgtg
     3901 tctagtgtac ctaaaagttc atcccagcta ttttcgtttc cattgcttcc gtgaagaaga
     3961 taaattacag gaaattgttt ttgagaggta tactcagccg gaagatatac tttatactta
     4021 aatgcatgtc cgagaatttt ggaatctatg cttgaacttt ctgtggttcc cttcagatca
     4081 agtgcatagc tcaatgtaaa aacagaaaac agaactgcaa taacagcta
//
LOCUS       DAOSHR010000176         4059 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_20069_length_4059_cov_4.671828, whole genome shotgun sequence.
ACCESSION   DAOSHR010000176 DAOSHR010000000
VERSION     DAOSHR010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 4059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4059
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20069_length_4059_cov_4.671828"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1415)
                     /locus_tag="PLU28_08865"
     CDS             complement(<1..1415)
                     /locus_tag="PLU28_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HOG35208.1"
                     /translation="MQDYINSSIDKSMKIKLEDKLPPMNDFLPGIRRAPKRQSDLTAE
                     QKVLAVKNALRYIPVQFHEQMMQEFLQELETTGRIYGYRYRPAGNIKALPINEYEGKC
                     LEGKAFQFMIDNNLDFDVALYPYELVTYGETGQVCQNWMQYRLIKKYLQELTKDTTLV
                     VESGHPLGLFRSHEYAPRVIITNGLMVGMFDNQKDFNVSAALGVANYGQMTAGGWMYI
                     GPQGIVHGTFNTILNAGRLKLGIAHDKDLKGKLFVSSGLGGMSGAQGKAGVIANAVAI
                     IAEVDISRVNTRKEQGWIDECVSGCKEAFDLAYKYVLAGQAKAIAYHGNIVDLLEYAV
                     NNNIHIDLLSDQTSCHAAYDGGYCPAGIDFEQRTFLLRENKNRFAELVNQSLKRHFEA
                     IKALTKRGTYFFDYGNSFMKAIFDAGVKEISKNGIDEKDGFIFPSYVEDILGPELFDY
                     GYGPFRWVYLSNDPKDLEKTD"
     gene            complement(1430..2959)
                     /gene="hutH"
                     /locus_tag="PLU28_08870"
     CDS             complement(1430..2959)
                     /gene="hutH"
                     /locus_tag="PLU28_08870"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HOG35209.1"
                     /translation="MEKIIVDGGRLKIEDVVNVARFMYEVGISQDALDKISQARQVVD
                     RYVEEERVVYGLTTGFGKFCDVFISKEQSIQLQKNLIMSHSCGVGNPLDQEIVRAIML
                     LRVNALSVGHSGIRASTVQTLIDMINKNVVPVIPEKGSLGSSGDLAPLSHMVLTMMGM
                     GEAFYENQRMDSEKAMKLAGIKPVELTSKEGLALINGTQVMTAIASLAVYDAENLSKS
                     ADVVSSLTIQALKGIVEAFDPKVHDIRNQQGQIKCAANLRKLLQGSELTTNQGEERVQ
                     DPYTLRCIPQIHGASKDAVEYVRTIVEREINAVTDNPLIFAEEDQVISGGNFHGQPIA
                     LAMDFLAIALSEFANVSERRIERLVNPQLSQLPAFLTKNGGLNSGFMITQYAAAALVS
                     ENKVLAHPASVDSIPSSANQEDHVSMGTIAARKAREILFNVTNVLAIELMASLQALDI
                     QEAADKLSPVTREVYDTCREQIKAVDKDRVMYTDINSAYRILKNGSIVDKSVKASGEI
                     Y"
     gene            complement(2976..>4059)
                     /locus_tag="PLU28_08875"
     CDS             complement(2976..>4059)
                     /locus_tag="PLU28_08875"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF013436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HOG35210.1"
                     /translation="TAASEIMAILCLATDMEDLKQRLGNIVVAYDADGKPVTARDLNA
                     QGALALLLKEAIKPNLVQTLENTPAIIHGGPFANIAHGCNSILATKMGLKLGEYLITE
                     AGFGADLGGEKFLDIKCRYGGLNPSAVVLVATIRALKMHGGVLKTELEMENVQAVQKG
                     FANLRKQVENIRKFNLPVLVAINKFSADTDAEIQALEEMCRSQNIDVVLNECWEKGGE
                     GGIEMAEKLVEMVQSGTCEYKPLYDLSSSLQQKVETIAQSIYGADSVVFSAQARKEIK
                     KLEKYGYDKLPVCMAKTQYSLSDNPDLLGEPKGFKITVKDVRVSAGAGFVVCLTGDIM
                     TMPGLPKIPAANVMDIDQDGKITGLF"
BASE COUNT         1087 a          995 c          711 g         1266 t
ORIGIN      
        1 ttatcggttt tttcaagatc tttgggatca ttgctgagat aaacccatct gaatggtccg
       61 tatccgtaat caaacagctc aggtccgagt atatcttcaa cataggaagg gaagataaag
      121 ccatcttttt catctatacc gtttttggat atctccttta ctccggcatc aaaaatcgct
      181 ttcataaagc tgttcccgta atcaaaaaag taagttcccc ttttggtcaa agcttttata
      241 gcttcaaaat gacgtttcag actttgattt acaagctctg caaacctatt tttattttcc
      301 cggagtaaaa atgtacgctg ttcaaagtct attccggcag gacagtaccc tccgtcataa
      361 gctgcatgac aggaagtctg atctgaaaga agatctatat gtatattatt attaacagcg
      421 tattccagaa gatctacaat atttccgtga taagctattg cctttgcctg tccggcaagt
      481 acatacttgt atgcaagatc aaaagcttcc ttacatccgc taacacattc atcgatccaa
      541 ccctgttctt ttcttgtatt tactctggat atgtcaacct cggcaattat agcaaccgca
      601 ttcgctatta cccctgcttt tccctgtgca ccgctcattc ctcccaaccc tgaagaaaca
      661 aaaagttttc ccttaagatc tttgtcgtgc gctatgccca atttaagcct tcctgcatta
      721 agaatagtat tgaacgtacc gtgaacaata ccctgcggcc ctatgtacat ccatcctccg
      781 gccgtcatct gcccgtaatt ggcaacaccc agtgcggcag aaacattaaa atccttttga
      841 ttatcaaaca ttcccaccat aagaccgttt gttattatga ccctaggtgc atactcatga
      901 gaacggaata acccaagcgg atgaccggat tcaaccacaa gggttgtatc ctttgtaagt
      961 tcctggagat atttttttat tagtctgtac tgcatccagt tctgacatac ctgacccgtc
     1021 tcgccgtagg ttaccagttc atacggatac agagcaacat caaaatcaag gttattatca
     1081 atcataaact gaaaagcttt tccttcaaga cattttccct catattcatt aatcggaaga
     1141 gcttttatat tgcctgcggg cctgtatctg tagccgtata tcctgcctgt tgtttcaagc
     1201 tcctgcagaa actcttgcat catctgttcg tgaaactgca caggaatata cctcagagca
     1261 tttttgaccg caaggacttt ctgttcagca gtcagatcgg actgcctttt aggtgccctt
     1321 cttattccag gcaaaaaatc attcatagga ggaagcttat cctccaattt tatcttcata
     1381 cttttgtcaa tactgctgtt aatataatcc tgcattctcc acccccattt taataaatct
     1441 cccctgatgc ttttacactc ttatctacaa tagagccatt tttcaatata cggtatgcac
     1501 tgtttatatc ggtatacatt actctgtctt tatctactgc ttttatctgt tcacggcagg
     1561 tatcataaac ctctctggtt acaggtgaaa gcttgtcggc agcttcctgg atatcgagcg
     1621 cctgaagcga tgccataagc tctattgcaa ggacattggt tacattaaat aaaatttctc
     1681 ttgcctttct tgcagctata gttcccatac tgacatgatc ctcctggttg gccgaagaag
     1741 gaattgagtc aacactggcc ggatgtgcaa gcactttatt ttcagatacg agcgcagctg
     1801 ccgcatactg ggttatcata aaacctgagt taagtccccc gttttttgta agaaaagccg
     1861 gaagctggct caactgtggg ttaaccaaac gttctatcct tctttcagat acatttgcaa
     1921 actcggaaag ggctatagct aaaaagtcca ttgccagagc tataggctga ccgtggaagt
     1981 tacctccgga aataacctgg tcttcctcag caaatataag cggattatcg gtaactgcat
     2041 ttatctccct ttcgacaatg gttctgacat actctacagc atccttgctt gctccgtgta
     2101 tctgaggaat acatctcaga gtataaggat cttgtactct ttcctcaccc tgattggtgg
     2161 taagttcact accctggaga agttttctga gattggcggc acactttatc tgaccctgct
     2221 ggtttcttat gtcatgtacc ttgggatcaa aggcttccac tattccctta agagcctgta
     2281 tggtaagtga agatacaaca tctgcacttt tggaaagatt ttcggcatca tacacagcca
     2341 gagaagctat agccgtcatt acctgtgtac cgtttataag tgcaagacct tccttggatg
     2401 taagctccac aggctttatg cctgcaagct tcatagcctt ttcggagtcc attctctgat
     2461 tttcataaaa agcttctccc atacccatca tagtcagaac catatgagaa agaggtgcaa
     2521 gatcgccaga tgaacccagt gagccttttt ccggaataac aggaactaca tttttattta
     2581 tcatatctat gagcgtctga actgtcgagg ctcttattcc cgaatgacca acagaaaggg
     2641 cattgactct caaaagcatt attgccctta ctatctcctg gtcaagagga ttacctacac
     2701 cacacgaatg actcattata agatttttct gaagctgaat gctctgttct tttgaaataa
     2761 atacatcaca aaactttcca aagccagtgg taagaccgta aacaactctt tcttcctcta
     2821 catacctatc aactacttgt cgggcctgtg aaatcttatc caatgcatct tgcgatatcc
     2881 caacctcgta cataaacctt gccacattaa ctacatcttc tattttaagc ctgccgccgt
     2941 caactattat tttttccact tagaacaact ccttttcaga ataatccggt aatcttaccg
     3001 tcttggtcta tatccataac atttgctgca ggaattttag gaagtcctgg catggtcatt
     3061 atatctcccg taagacaaac cacaaatcct gctccggcag ataccctcac gtccttgaca
     3121 gttattttga atcccttagg ctcacctaaa agatcgggat tgtctgaaag agagtactgt
     3181 gtctttgcca tacataccgg aagtttatca tatccgtatt tttcgagttt tttgatctct
     3241 ttccttgcct gtgcggaaaa aaccacactg tcagctccgt atatactttg tgcaatagtc
     3301 tcaaccttct gctgcagact actactcaga tcatataaag gcttgtactc acatgttccg
     3361 gactgaacca tctcaacgag tttttcggcc atctcaattc cgccttcccc gcctttttcc
     3421 caacattcgt tcaaaaccac gtctatattc tgtgatctgc acatctcttc caatgcttgt
     3481 atttcagcat ctgtatcagc cgaaaatttg tttatggcaa ccaaaacagg gagattgaac
     3541 tttcttatat tttcgacctg ctttctgaga ttggcaaaac ctttttgtac agcctgaaca
     3601 ttctccatct caagttctgt ctttagtact cccccatgca ttttcagtgc tcttatggtt
     3661 gcgacaagca caacagctga aggattaagc cctccatagc ggcactttat atcaagaaat
     3721 ttttctcctc ccagatcagc accgaatccg gcttcggtta taagatactc accaagctta
     3781 agacccattt ttgtggccag tatgctgtta caaccgtgag caatattagc aaaaggccct
     3841 ccatgaatta tagcgggagt attttccaga gtctggacaa gattaggctt tatggcttct
     3901 tttaacagaa gagctaatgc cccctgtgca ttgagatctc ttgccgttac aggtttgccg
     3961 tctgcatcgt atgccacaac aatattaccg agtctctgtt taagatcttc catatctgtg
     4021 gcaagacaga gaatcgccat tatctctgaa gctgcagtg
//
LOCUS       DAOSHR010000177         4052 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_20107_length_4052_cov_5.990243, whole genome shotgun sequence.
ACCESSION   DAOSHR010000177 DAOSHR010000000
VERSION     DAOSHR010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 4052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4052
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20107_length_4052_cov_5.990243"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1146
                     /locus_tag="PLU28_08880"
     CDS             <1..1146
                     /locus_tag="PLU28_08880"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HOG35211.1"
                     /translation="KLFFISGRAGTGKTTLVLSYLKGLTGKGIITQNDIMLLAPTGKA
                     ADRLKIVTGFQARTIASVLYENGWYVSLKNGFLMKRRNGGKKVRKRIIIVDESSMIDM
                     ASFRAVLSTVDLSYLMQVIFVGDKNQLPPVGAGKVFSQLIHELPGNLFELKVVNRFDD
                     QSPQSGLARAVLEGTSKTGMDFSSFAKKYSSVRELGSLILEQISQYEKKREEWIILTP
                     NNIGPFGVNALNLMIKKSIFGYDIKGYNLNERVILLENLPERKVYNGQIGYVEKVFDP
                     FQDEFYIQVSFENGIKANFGAYEAGLLLNSAYAVTIHKAQGSEFENVIVVLPARSRLL
                     SKELIYTAFTRFRKDLRVYIQQEDLKSFIDGLPSALQEIKSRKLIDG"
     gene            1247..2443
                     /locus_tag="PLU28_08885"
     CDS             1247..2443
                     /locus_tag="PLU28_08885"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOG35212.1"
                     /translation="MLILFVWILTFRNIRMFVFLLIFLVPFIYADISRIEGDVGVLGR
                     VVDRRNGSYTLISTHIYNGAWKKYRDYIKFDYYKFSTEKISCGKNVYVSGQLKEGYIN
                     ATYAAPSVDSSFFRIKEKALENLSLAVKDKEAFDIISASFMGSLKDKEVFKSSGTLHL
                     FAVSGMQVYLFSATVFFILRYLVRKRSLRLLINMGMVWVYMILTGFSPSSMRAGLLII
                     GVCLFKILDIGVSSFNILGLIGYLSLVFMPLNIYNVGFQMSYSSAFMILFSVRATAGL
                     KKKKLFPLFRAAVIAVGAFLGVIPFCIAYFGSFSILGPFLTPLVEPVLDILMILCMVM
                     VLVPLEQLGVVASFLALACKAFLKTVSFMPLTTVENRLYGLLISCTILWAYIFMLIRI
                     EKRYKR"
     gene            2515..3222
                     /locus_tag="PLU28_08890"
     CDS             2515..3222
                     /locus_tag="PLU28_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35213.1"
                     /translation="MGRTFLKKIVNGIAFKISALFTVIIISLLILTGFFTDKMIKKTI
                     ELTVVNRSEEISEKSSGMIDKAKFSEIIKLIENQKDTEDISATANFSDTLNLIRTIRD
                     ANGLKFLYTMTKTDNSYVYVVSSDYGQENGSSPGDVEENYVETFEKVYKTGKSQLDIS
                     NDSYGYLISTISPIFDERGSIIGLLGADFDVSQIMKMIDKERLVLNLSMVLALLISVA
                     VAVFFSLRLTEPIKALV"
     gene            3256..>4052
                     /locus_tag="PLU28_08895"
     CDS             3256..>4052
                     /locus_tag="PLU28_08895"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOG35214.1"
                     /translation="MSADMSRKDEVGELAQSFCLMISDIKRVIVSIKENASEILSLSE
                     ELSDSAQTTTDASNSIAENVQKRVSDIELLLDKSDYAGSLLSEMSEKAQKISQKTFLV
                     SQVSQNTKDQATAGNTQIYRSIEQMNEIDKALSESRSIISEMSALYREISEFVQTISQ
                     IAAQTNLLSLNASIEASRAGEHGKGFAVVADEIKKLAQQSKEAALMIQDIISKVGENS
                     DKSIQSMEKLSTEVKKGINSIGQSSEAFKSIFESSVRTAQEIKEVTVS"
BASE COUNT         1230 a          649 c          883 g         1290 t
ORIGIN      
        1 aagctttttt ttataagcgg aagggcaggc acaggaaaga caactttagt tctttcgtat
       61 ctcaaaggtc tcacgggtaa aggaattata actcaaaacg atataatgct tttggctccg
      121 actggcaagg ccgcagacag gcttaaaata gtaaccggct ttcaggccag gacgatagca
      181 agtgttttat atgaaaacgg atggtatgtc tcgctaaaaa acggattttt aatgaaaaga
      241 agaaacggcg gaaaaaaggt gcgtaaaaga atcataatcg tagacgagtc gtctatgatt
      301 gatatggcat cttttagagc ggttttaagc actgttgacc tttcgtatct tatgcaggta
      361 atctttgtag gcgataaaaa tcagcttcct cctgttggag caggaaaggt gttcagtcag
      421 cttatacatg agctacctgg caatctgttt gaactcaagg tagttaaccg ttttgatgat
      481 caaagtcctc agagcggact tgctagggct gtgcttgaag gaacatctaa aacggggatg
      541 gatttttctt cttttgcaaa aaaatactct tcggttaggg agcttgggtc tctcattttg
      601 gaacagatat cgcaatatga aaaaaagagg gaagaatgga taattttgac tcctaataat
      661 ataggtccct ttggggtcaa tgcgcttaac ctgatgataa aaaaaagtat tttcggatac
      721 gatataaagg ggtacaacct gaatgaaagg gtaatacttc tggaaaatct tccggaacgt
      781 aaagtttaca atggccagat aggatatgta gaaaaggtat ttgatccttt tcaggatgag
      841 ttttacatac aggtttcctt tgaaaacggt ataaaagcaa actttggagc atacgaggct
      901 ggacttttgc ttaacagtgc ctatgcggtt acaatacaca aggctcaagg aagtgagttt
      961 gaaaacgtta tagtggtact tcccgccagg agcagacttt tgagtaaaga gctcatatat
     1021 acagctttta ccaggttccg taaagatctc agagtatata ttcagcagga ggatttgaag
     1081 agcttcatag atggtctgcc ttcagcattg caggagataa aatcaaggaa gctgattgat
     1141 ggttaaaggc agagggctac ctcctttctt ttttcagttg tttttaattt tttctgtttt
     1201 gcttttacta aaaagtataa atttttgggt ttcattggct gtatgtatgc tgatactgtt
     1261 tgtttggatt ttgactttca gaaatatacg tatgtttgtt tttcttctga tcttcctggt
     1321 gccttttata tatgctgata tttcaaggat tgaaggagat gttggtgttt taggcagagt
     1381 ggtagacaga agaaacggct cttatacgct tataagcact catatataca acggtgcatg
     1441 gaagaagtac agagactaca taaagtttga ttattataag ttcagtacag aaaagatcag
     1501 ctgcggtaag aatgtatatg tgtcaggtca actgaaagaa ggttatatta atgccacata
     1561 tgcggcacct tcagtcgatt cttctttttt cagaataaag gaaaaggcac ttgaaaatct
     1621 ttctttggcg gtaaaggaca aagaggcatt tgatataatt tctgcttctt ttatggggag
     1681 tcttaaggat aaagaagttt ttaagagttc gggtacgctt catctttttg cggtttcagg
     1741 gatgcaggtt tatctttttt ctgcaacagt attctttatt ctgaggtatc tggtcagaaa
     1801 acgaagtttg cgtttgttaa taaatatggg tatggtatgg gtttatatga ttttgacggg
     1861 tttttctcca agttctatgc gtgcggggct tcttattatc ggggtatgtc tttttaaaat
     1921 tcttgacata ggcgtaagtt catttaacat acttggtctt ataggctatc tttctttggt
     1981 gtttatgccg ctgaacattt ataacgtagg atttcaaatg agttattcat cagctttcat
     2041 gatactgttt tcagttcggg ccacagctgg tctcaaaaag aaaaagttat ttcctttgtt
     2101 tcgcgctgca gtaatcgctg tcggtgcctt tcttggagtg attccttttt gtattgcata
     2161 ttttgggagc ttttcaattt taggaccatt tttaacacca cttgtggagc ctgttcttga
     2221 tattctaatg attctatgta tggttatggt tttggttccg cttgaacaac tgggggtagt
     2281 ggcttctttt cttgccctgg catgcaaagc ttttttgaaa actgtttctt ttatgcctct
     2341 taccacagtt gaaaacaggc tgtatgggct tttgatatcc tgcacaattc tttgggcgta
     2401 tattttcatg cttattagga tagaaaaaag atataagcgt tgaaaatatc tttataaaaa
     2461 ctgcaaaaag ttttattatg ttgagcttca ctttaaaatt cgggagctga tattatgggc
     2521 agaacttttt tgaaaaagat tgtgaacggc atagctttta aaatttcggc actttttacg
     2581 gttattatta tttctttact tattctgact ggatttttca ctgacaagat gattaaaaaa
     2641 acgattgagt tgacagttgt caatcgttct gaggagatat ctgaaaaatc ttccggaatg
     2701 attgataagg ctaaattttc agaaatcata aaacttatag agaatcagaa agatacagaa
     2761 gatatatctg cgacagctaa tttttcggat acgctgaact taatcagaac cataagagat
     2821 gcaaacgggc taaaattctt atacaccatg accaagacgg ataacagtta tgtttatgtt
     2881 gtttccagcg attatgggca ggaaaacgga tcttcgccgg gtgatgtgga agaaaattat
     2941 gttgagactt ttgaaaaggt ttataaaact ggtaaatctc agcttgatat atcaaatgac
     3001 agttatggtt atcttataag cactatatcg cctatttttg atgaacgggg cagtattatc
     3061 gggcttttgg gcgcagattt cgatgtgtct caaataatga aaatgataga taaagagaga
     3121 ttagttctta atttgagcat ggtacttgct cttcttattt ccgtggctgt agcagtattc
     3181 ttctctttgc gcctgacaga accaataaaa gcgctggttt agaagtcaaa gctcataagt
     3241 accggagacc tttccgtgag tgctgatatg tcaagaaaag acgaagtcgg agaacttgca
     3301 caatcattct gtttaatgat atctgacata aagagagtaa ttgtttctat aaaagaaaat
     3361 gcttctgaaa ttctttcgct ttctgaagag ctttcggatt cagctcaaac tactacagat
     3421 gcaagcaaca gtatagcaga aaatgttcag aagagagttt ctgatattga actgctatta
     3481 gataaatcag attatgcagg cagtctcctc agtgaaatgt ctgaaaaagc acagaaaatt
     3541 tcacaaaaaa cctttttggt ctctcaggta tcccaaaaca ccaaggatca ggccacggca
     3601 ggaaacactc agatataccg ttcaatagaa cagatgaatg aaatcgataa ggcgctttcc
     3661 gaatcacggt caataatatc tgaaatgtcc gcactttaca gggagataag tgaatttgtc
     3721 cagactattt cgcagattgc cgcacagact aatctgcttt ctcttaacgc atctattgaa
     3781 gcttctagag ccggtgagca cggaaaaggg tttgcagtag ttgccgacga aataaaaaaa
     3841 cttgctcagc agtcaaagga agcggcatta atgatacagg atattatttc caaggtagga
     3901 gagaactcag acaagtctat acagtccatg gaaaagctga gtacggaagt aaaaaaaggt
     3961 attaactcta tagggcagag cagcgaagca tttaaatcca tttttgaatc ttcagtccgg
     4021 acagcccaag aaataaaaga ggttacagta tc
//
LOCUS       DAOSHR010000178         4051 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_20116_length_4051_cov_6.023023, whole genome shotgun sequence.
ACCESSION   DAOSHR010000178 DAOSHR010000000
VERSION     DAOSHR010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 4051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4051
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20116_length_4051_cov_6.023023"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            188..1675
                     /locus_tag="PLU28_08900"
     CDS             188..1675
                     /locus_tag="PLU28_08900"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOG35215.1"
                     /translation="MSGFSAVDTAIGYMEKNIFEDIDFSVLCENVGYSISYFYEVFYK
                     KTGQTPAAFLRNKRLAYSCWDLAFSNLKILDISVKYRFSSQESFTRAFSKKFEITPGR
                     FRKEIKKNFDKKKFSTEAEKNMEETLKSFPDKKVLKDVMKVGFYSGSDICPEDVPFPS
                     CLASVLRYAGEDYPWIPVLEHNRTWKLNYPYVLFMGVSGMAFGFLWKKGWHQDNVDHM
                     FIADPSQIIDRAFRAAGYDYETVDKSGVQNDEKLFRQKIMYSINKNLPVLAFGVVGPP
                     ECSIITGYDKNCDKVLGWSFFQNFEVFNDGVSFDENGYFIKSDWFANTWTLLILNGKN
                     PGKKEINFSDTMDWIVKVLTTEDLYGRKAGIAGLQAWREQILDGNDFIEISEEKLKMM
                     HEIHNSAVGMFAECRWIAGLFLRDLAAKNTKFEYELDQAASIMKLQHDLMYNMWDICG
                     GIYNPNSWEFFGKSDVRRELSKVIEEVISNEKKMIDIFRKISSRY"
     gene            1746..2621
                     /locus_tag="PLU28_08905"
     CDS             1746..2621
                     /locus_tag="PLU28_08905"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HOG35216.1"
                     /translation="MTYTIKNEYLQVSLDSKGAEMTSLVDKAGINYLWTADPKHWARH
                     APVLFPVVGKLKNNRYTYRQKEYSLGQHGFARDLEFEALSNFNSSVEFLLKSSPYTKE
                     KFPFDFELSIRYALEKNKIHIFYNVRNKDTLAMLFSIGAHPGFRCPLYDNESIKDYYI
                     QFNKSETAYIMLLNHETGLFERETALLLKDEDKIFLDQTTFEHDALVFKGLRSTQATI
                     KSPKNQHSVTVGFEGFPYLGLWSKPGAPFVCIEPWFGHADFKDSDGNFESKEGLISLK
                     PLQSFECSFFIQLDS"
     gene            complement(2699..>4051)
                     /locus_tag="PLU28_08910"
     CDS             complement(2699..>4051)
                     /locus_tag="PLU28_08910"
                     /inference="COORDINATES: protein motif:HMM:NF024683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FN3 associated domain-containing protein"
                     /protein_id="HOG35217.1"
                     /translation="EPSTSSSLYTGAINISSSKILKARAFKHGWVESDIVTQTYTFSV
                     SLALSKCFGGIYNDYLNSIIQTSDGGYMAVGDMNSTDGDFSANHGDYDGWIIKVDSNG
                     NKLWIKCFGGNSTDLLRSIIQTSDGGCIAVGYTKSSDGDLSGSRDSLDGWIIKLDSNG
                     NKLWSNCFGGSGNDYLNIIVQTADGGYIAAGETLSSDGDFSEHHGGYTDGWIIKLDSN
                     GNKLWSKCFGGSDDDSLKGIIQLSDGGYIAIGSTMSTDGEFSGHNGGRTDGWIIKVSS
                     AGNKLWSKCFGGNGNDYLSNITQTSDGGYIAAGETSSTDGDFSGNHSGKTDGCMIKFD
                     AADNKVWSKCFGGTDYDSLTDIIQTIDGGYMASGYAKSNDGDVSDSHGDYDGWIIKVN
                     SSGNKEWSKCFGGSNIDYLYKLLQTSDNGFCTVGYTKSNDGDVSGYHSNRDGWILKLE
                     "
BASE COUNT         1284 a          776 c          755 g         1236 t
ORIGIN      
        1 cgtgtcacag ggtaatagga actaacggga agctgacagg ctacagcggt gggttatgga
       61 gaaaaaaatt cctgatagaa cttgaaaaag agatctctgc ccaaaaactt ttttaagacc
      121 ggaaaaaaaa tcaaaaattc ctgaattatt tattttataa tctatgtgtc cggacaagga
      181 ggcagaattg agcggttttt cagcagtaga cactgcaata ggctatatgg aaaaaaatat
      241 tttcgaagat attgactttt cagttctttg tgaaaatgtt ggttacagta tatcctactt
      301 ttatgaggtg ttttataaaa aaaccggtca gactcctgcc gcttttttga ggaataaaag
      361 gcttgcctat tcatgttggg atctggcttt ttccaatctt aagatacttg atatttcagt
      421 taagtatcgc ttttcatctc aggagtcttt tacaagagcg ttttcaaaaa aatttgaaat
      481 cactccggga agattcagaa aagaaatcaa aaaaaacttt gataaaaaaa agttttcaac
      541 ggaggcagaa aaaaatatgg aagaaactct taaatccttt ccggataaaa aggttttaaa
      601 agatgtcatg aaggttggtt tttactcagg ctcggatatc tgtcctgaag atgttccctt
      661 tccgagctgt cttgcaagcg tgctacgcta tgctggtgaa gattatccat ggatacctgt
      721 tctggagcat aacagaactt ggaagctcaa ctatccttac gttttattta tgggtgtaag
      781 tggaatggct tttggatttt tatggaaaaa gggctggcac caagataatg tagatcatat
      841 gttcatcgcc gatccttcac agataataga tcgtgcattc agagcagcgg ggtatgatta
      901 tgaaaccgta gacaaatcag gagtacaaaa tgatgaaaaa cttttcaggc aaaaaataat
      961 gtactccata aacaaaaatc tcccggtact tgcttttgga gtagtcggcc cgccagaatg
     1021 ttcaataata acaggatatg acaaaaactg cgataaagta ttaggctgga gtttttttca
     1081 gaactttgaa gtttttaatg acggggtcag cttcgatgaa aacggctatt ttataaaatc
     1141 tgattggttt gcaaatactt ggacactttt aattcttaat ggaaaaaatc ctggaaaaaa
     1201 agaaattaac ttctctgata ctatggattg gattgttaag gtacttacca cagaagacct
     1261 ttatggaaga aaagcaggaa tagctggatt gcaggcatgg agagagcaaa tactcgatgg
     1321 aaatgacttt atagagattt cagaagaaaa acttaaaatg atgcacgaaa ttcataactc
     1381 tgcagttgga atgtttgctg aatgccgatg gatagccgga cttttcctga gagatcttgc
     1441 agctaaaaat acaaaatttg agtacgaact tgatcaggcc gcttcaatca tgaaactgca
     1501 acatgatctg atgtacaata tgtgggatat atgtggagga atatacaatc ctaactcatg
     1561 ggagtttttt ggcaagtctg acgtaagaag ggagctttca aaagtcatag aagaagtaat
     1621 ttcaaatgaa aaaaagatga tcgatatatt taggaaaata tcttcacggt actgaaaaac
     1681 acaggctaag cctgtgtttt tcagtgcatt gtgtaatata ttaatcaaaa tttttgaggt
     1741 ggaagatgac atatactata aaaaatgagt atctgcaagt atctttggac agcaagggcg
     1801 ctgaaatgac atctctggta gataaagccg gtatcaacta cctgtggact gctgatccaa
     1861 agcactgggc aagacatgct ccggttcttt tcccggttgt gggaaagctt aagaataaca
     1921 ggtatactta ccgccagaaa gagtattctc tgggtcagca tggttttgca agagatctgg
     1981 agtttgaagc tctatctaat ttcaacagtt ccgttgaatt tcttctaaaa agcagtccct
     2041 atacaaaaga aaaatttcct tttgattttg agctttctat ccgttatgct cttgagaaaa
     2101 ataaaatcca tattttttat aacgtaagaa acaaagatac tttggctatg ctcttttcta
     2161 taggagcaca tcccggcttc agatgtccct tatacgataa cgaatctatt aaagattact
     2221 acatacagtt caataaatcg gaaacggcat atattatgct tttaaaccat gagacgggac
     2281 tttttgaaag agaaactgct cttttgctta aggatgaaga taaaattttt cttgaccaaa
     2341 ccacttttga acacgatgca cttgtattta aaggacttag atcaacccag gccactataa
     2401 aaagccctaa aaaccaacac tctgtaactg ttggctttga aggatttcca tacctaggat
     2461 tatggtcaaa gccaggtgca ccattcgtat gtattgagcc atggttcgga catgctgact
     2521 tcaaagattc tgacggcaat tttgaaagta aagaaggact tatctcctta aaacctttac
     2581 agtcatttga atgcagtttt tttatacagc tggattcata gccgaataac ttaagttaac
     2641 gcatcgataa aaatcaacct ccaactggag ttatctcaag ttggaggttg attgtttact
     2701 actctaattt aagtatccag ccatcacgat tactgtgata accagaaaca tctccgtcat
     2761 ttgattttgt atatcctaca gtacaaaaac cgttatccga tgtttgaagt aacttatata
     2821 aataatcaat gttacttccg ccaaagcatt ttgaccattc tttattgcct gatgaattaa
     2881 ccttgattat ccacccatca tagtcaccat gactatctga aacatctcca tcgtttgact
     2941 ttgcatatcc tgacgccata tacccgccgt ctattgtctg gattatgtcg gttaaagaat
     3001 catagtcagt tcctccaaag cattttgacc acactttatt gtctgctgca tcaaacttga
     3061 tcatacaacc gtctgttttc ccgctgtgat tacctgaaaa atctccatca gtcgacgagg
     3121 tttccccagc agctatatat ccgccatctg atgtttgagt tatattgctt aaatagtcat
     3181 tgccatttcc gccaaaacat tttgaccata gtttatttcc agctgagctg actttaatta
     3241 tccacccgtc ggttctgcca ccgttatgcc ctgaaaactc tccatcggtt gacattgtgc
     3301 ttccaatagc aatatacccg ccgtctgata attggattat acctttcaaa gaatcatcat
     3361 cgcttcctcc aaaacatttc gaccagagct tattgccgtt tgaatccaat ttaattatcc
     3421 acccgtcagt atagccacca tgatgctctg aaaaatctcc atcgctagat aatgtttccc
     3481 cagcagcaat atagccaccg tcagctgtct gaactataat atttaaataa tcattaccgc
     3541 ttcctccaaa acaattcgac caaagcttat tgccgtttga atcaagcttg attatccagc
     3601 catcaagact gtcacggcta ccagaaagat ctccatcact tgatttggtg taaccaactg
     3661 caatacagcc gccgtctgat gtctggatta tactgcgcaa aagatcagtg gagtttcctc
     3721 caaagcattt tatccagagc ttattgccgt ttgaatcaac tttaattatc cacccgtcat
     3781 aatctccgtg attggccgaa aaatctccat cggttgagtt catatctcca acagccatat
     3841 aaccaccatc cgatgtctga attatactat ttaaatagtc gttgtagatt cctccaaagc
     3901 atttgcttaa agctaaagat actgagaagg tataagtttg tgttactata tcgctttcaa
     3961 cccatccatg tttaaaagct cttgctttta atatcttaga agaagatata ttgattgctc
     4021 cggtgtataa gctgctggat gtagagggtt c
//
LOCUS       DAOSHR010000179         4020 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_20351_length_4020_cov_3.900883, whole genome shotgun sequence.
ACCESSION   DAOSHR010000179 DAOSHR010000000
VERSION     DAOSHR010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 4020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4020
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20351_length_4020_cov_3.900883"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="PLU28_08915"
     CDS             complement(<1..472)
                     /locus_tag="PLU28_08915"
                     /inference="COORDINATES: protein motif:HMM:NF014500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HOG35218.1"
                     /translation="MSIWFFILAAVTVAMVVLLTAWLKVHPFLSMLGACFFFAIASGI
                     PTQSIGKYITDGFAETFGSIGIVIIIGSIIGTFLQRSYGSMAIAQSILKLTGKKHVPL
                     AMSLMGYFTSICVFCDSGFVMLSPLNRELSRRSGQSIALTSTALSLGLYATHTLV"
     gene            689..1753
                     /locus_tag="PLU28_08920"
     CDS             689..1753
                     /locus_tag="PLU28_08920"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOG35219.1"
                     /translation="MDFYLNLSINSKEKNELIIEDYFRWFRKGDNVNLTMFFSGDTQM
                     EVYKRKVHDLCTRKDFPKYSIVTEKVSFSGLKEFYSKPVLLNEIINYNRDDIFRIYKL
                     MNPTVKECDFENQPISIQYDIFYDRHLFGDARGYSPETTVQIKDMIDFLFPVKILDAG
                     CGCGANYFYLKDALKDGTSYHGVDFSRYNIIKAIDYFSGDNVSFSFGDIRSLEFDDGF
                     FDFSFTESVLPYVSDPLRAVKELYRVSEKGFYAAFYSVEQKIKGCRFNKDTFCYELDT
                     GASWKFYKNTPNTFYLPRFSDAIRVLEGMEDKLSIRINQKDQFFGKIGINTKNIFIYP
                     KAWYESSCNTYKKWNNKPLM"
     gene            1785..2714
                     /locus_tag="PLU28_08925"
     CDS             1785..2714
                     /locus_tag="PLU28_08925"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOG35220.1"
                     /translation="MKTETFKFKDPHGYEIFCYKWYEPEQRPRAVVQIAHGMAEHALR
                     YEKFAGFLTAKGFIVYANDHRGHGQTAGELGNVGYLGDDDGFEWMVKDLHQLSGIIKK
                     EYQLLPVFLFGHSMGSYIVRRYIEIFSKDINGAVLCGSGAIGMLEKIGSFLAKNEVRR
                     NGPKTPSPKLNNLTFGSYNKKFVPNRTEFDWLSRDTEQVDKYVADRYCGGVFSCKFFY
                     DLAQGLVNLNKLENMEKINKELPLLFISGSEDPVGKFSKAMPKILHRYHKAGMKDIQH
                     RLYLGARHELLNEINKDEVMNDIYEWIDKKISR"
     gene            complement(2725..3327)
                     /locus_tag="PLU28_08930"
     CDS             complement(2725..3327)
                     /locus_tag="PLU28_08930"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF014228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HOG35221.1"
                     /translation="MKKILCLTGAGISKASGIPTFTEMEDIRVKLSRTYYKSNPEDFF
                     EVLIKMKDNIDSAQPNPAHIALARFKVPIVTMNIDGLHQKAGSTDVIELHGNLDFVEC
                     KKCGSRYPFSAVRKSILCPVCGGVLDTNVVLYGDMIRDYDKACTRASQADEVLIIGTS
                     FYTSTAIGISQVAQLNQIPTTIINENAEENVEKYLISVFG"
     gene            3477..3755
                     /locus_tag="PLU28_08935"
     CDS             3477..3755
                     /locus_tag="PLU28_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensing transcriptional repressor"
                     /protein_id="HOG35222.1"
                     /translation="MNKIHHEEALKTLKTAKGQIESTIKMIEENRYCIDISKQVLAVT
                     ALLKKANIQILKKHLESCVLDAALSKDSAEKSIKIKELEDTLEYINRV"
     gene            3770..>4020
                     /locus_tag="PLU28_08940"
     CDS             3770..>4020
                     /locus_tag="PLU28_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HOG35223.1"
                     /translation="MENEYKKSFSVQGMTCASCVRNVENTLKKMPGVKYVSVNLATEQ
                     AYLISEKEISFQEIKKAVEESGYTAMSKVPDGSVIEKRF"
BASE COUNT         1397 a          685 c          848 g         1090 t
ORIGIN      
        1 gaaccagtgt gtgggttgca tacaggccaa gacttaaagc agttgaagtt aaagcaattg
       61 attgacccga tcttctgctc aactctctgt ttagaggcga aagcattaca aaaccagaat
      121 cacaaaatac acatattgat gtaaagtaac ccatcaaaga catggcaaga ggcacatgtt
      181 ttttccctgt aagttttagg atgctttgag caatggccat tgagccgtaa gaccgttgaa
      241 gaaaagttcc tattatcgat cctataataa ttacaattcc tatggaacca aatgtttcag
      301 caaaaccatc tgttatgtat ttccctatgc tctgtgtcgg gataccgctt gcaattgcaa
      361 agaaaaagca agctccaagc attgaaagaa acgggtgaac ctttaaccaa gcggtgagta
      421 aaacaaccat cgcaacagta actgcagcaa gaataaaaaa ccatatgctc attttttgcc
      481 tccgtagatg aatcattcac ttatatttta tctgataatt atactcaaag caaccatgaa
      541 gttaaaaaaa gtacttagac gcatgaccat agaacccaaa agaaaaagaa ataagttata
      601 ataagtataa gagtagtttg ataagaacca tgaaacaaaa atagataata aggctataca
      661 atactataaa gggttaaagg atgggaaaat ggacttttat cttaatcttt ccataaacag
      721 caaagagaaa aatgagctta taattgagga ttactttcgt tggttcagaa aaggagacaa
      781 tgtaaatttg actatgtttt tcagcggaga tacccagatg gaagtataca agcggaaagt
      841 tcatgatctt tgtactcgaa aggattttcc gaagtacagc atagtcacag agaaggtaag
      901 tttttccgga ctgaaagaat tttattccaa gccggtactc ctaaatgaga ttataaacta
      961 caaccgggac gatatattta gaatttataa gctgatgaac cccaccgtaa aggaatgtga
     1021 ctttgaaaac cagccaatct caatacagta cgatattttt tatgaccgcc atctgtttgg
     1081 ggatgcacgt ggatacagtc ctgaaacaac agttcagata aaagatatga tagactttct
     1141 ttttccggtg aaaattttgg atgccggctg cggatgcggt gccaactact tctatctcaa
     1201 ggatgcactc aaagatggaa cttcctatca tggagtggac ttcagccggt ataacataat
     1261 aaaagcaatt gattactttt caggtgataa tgtcagcttt tctttcggag atataaggtc
     1321 gttggagttt gatgacggct tctttgattt ttcttttacc gaaagtgtgc tgccgtatgt
     1381 ttcagatccc ttacgggcgg ttaaagagct ttacagggta tccgaaaaag gtttttatgc
     1441 tgcattctac tcagtagaac agaaaataaa agggtgccgt ttcaataaag atacattttg
     1501 ctatgagctg gataccgggg caagctggaa gttctataag aatacaccca acacctttta
     1561 tctgccaaga ttttccgatg ccataagagt tttagagggg atggaagaca agctaagtat
     1621 aagaataaac cagaaggatc agttttttgg caaaataggt atcaatacca aaaatatttt
     1681 catatatccc aaggcatggt atgaaagcag ctgcaataca tataaaaaat ggaataataa
     1741 gccgctgatg taaaaaagcc aaaaggtgaa atcggggagg aagaatgaag acagaaactt
     1801 ttaaatttaa agatcctcat ggatatgaga ttttctgtta caaatggtat gagccggagc
     1861 agagacccag ggcagtggtt cagatagctc atggaatggc cgagcatgca cttagatatg
     1921 aaaaatttgc gggtttttta actgcgaaag gttttattgt gtatgccaac gaccacagag
     1981 gacacggtca gacggcaggg gaacttggaa atgtaggata tcttggtgat gatgatggat
     2041 ttgaatggat ggtaaaggat ctgcatcagc tgagcggaat aataaaaaag gagtatcaac
     2101 ttcttcctgt gtttcttttc ggacacagca tgggatctta tatagtaagg cgctatattg
     2161 agattttttc caaagatatt aatggtgcag tactttgcgg aagcggtgcc ataggaatgc
     2221 tggaaaaaat aggcagcttt cttgccaaaa atgaggtcag gaggaacgga cccaaaactc
     2281 caagtccaaa actcaataac cttacttttg gcagttataa taaaaaattt gtacccaaca
     2341 ggacagagtt tgactggctg agccgtgata ccgagcaggt agataagtat gtagcggaca
     2401 gatactgtgg cggggttttt tcatgcaaat ttttctatga tcttgctcag ggactggtta
     2461 atctcaataa acttgaaaat atggaaaaaa taaataaaga gcttccctta ctgtttatct
     2521 caggaagcga agaccctgtc ggcaagtttt caaaagcgat gcccaaaata cttcacagat
     2581 accataaagc cggtatgaag gatatccagc acaggctgta tctcggagca agacatgagc
     2641 tactgaacga aataaacaaa gatgaggtca tgaacgacat atatgaatgg atagataaaa
     2701 aaataagccg ttaggcttat ttttttatcc gaataccgaa ataagatact tttcaacatt
     2761 ctcttctgca ttttcattta tgatggtggt gggaatttga ttaagttggg caacctgaga
     2821 tattcctatt gcggttgagg tatagaaaga agttccgatt ataagaactt catctgcttg
     2881 tgaagctctt gtacaggcct tatcgtaatc acgtatcata tcgccataca aaacgacatt
     2941 tgtatcaaga actccgccgc agacaggaca taaaatactt ttcctgacag ctgaaaaagg
     3001 atatctggaa ccacattttt tacactccac aaagtcaaga ttaccatgca actctatgac
     3061 atctgtactt cctgcttttt ggtgaagacc gtcgatattc atggtgacta tgggcacttt
     3121 gaatctggct aaggcaatat gggcaggatt aggctgtgca gagtcgatat tatccttcat
     3181 ctttataaga acctcaaaaa aatcttccgg attggatttg tagtaggtgc ggctaagctt
     3241 aacacggata tcctccattt cggtgaaggt cggaatgccg ctggccttag atattccggc
     3301 tcccgtaaga cataaaattt ttttcataaa tatcacctca taacgattat agcagaaata
     3361 aataccagag tgaaaaatat tccagataaa aaaaactata atgtaacctg aaaataatac
     3421 taaaaaggta ggatatatta acccccctag gtagggtagt atgatatgga ggagatatga
     3481 ataaaataca tcatgaagaa gctttaaaaa cgctgaaaac tgcaaaagga cagatcgaat
     3541 cgactataaa aatgatagaa gagaacaggt actgcataga tatttccaag caggtgctgg
     3601 cagtaacggc acttcttaaa aaagccaata ttcagatact gaaaaagcac cttgaaagct
     3661 gtgttttaga cgctgctctc tctaaggatt cagcggagaa atcaataaaa ataaaagaac
     3721 ttgaagatac tttggagtat ataaacaggg tataaaccgg aggtggaaaa tggaaaatga
     3781 atataaaaaa agtttttctg tccagggaat gacatgcgca tcgtgcgtaa ggaatgtaga
     3841 aaacacttta aaaaagatgc ctggagtaaa atatgtatca gttaaccttg cgacggaaca
     3901 ggcatatctt atttcagaaa aggagatttc ttttcaagag ataaaaaaag ctgttgaaga
     3961 gtcgggatat acggccatgt ctaaagtacc agacggttct gtaatagaga aacgattttt
//
LOCUS       DAOSHR010000180         4019 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_20359_length_4019_cov_4.982341, whole genome shotgun sequence.
ACCESSION   DAOSHR010000180 DAOSHR010000000
VERSION     DAOSHR010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 4019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4019
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20359_length_4019_cov_4.982341"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="PLU28_08945"
     CDS             <1..516
                     /locus_tag="PLU28_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35224.1"
                     /translation="LKLGSVIEKAFGKKVFNIDIYLSEFKKEFGVKPHNARNFDFVSF
                     YQIVLLKDVLTQKTEQLMLEYILNKDNGIYYIYNKKLSQLPAEFKSLQTVRYMDAVEL
                     IAEYRYSSDCLAFVSDWLKHHVEEDGSWDLGAKAKDNISFPISDSWRSPNSRKKDCTA
                     RVTRLLRMIQK"
     gene            complement(528..2834)
                     /locus_tag="PLU28_08950"
     CDS             complement(528..2834)
                     /locus_tag="PLU28_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOG35225.1"
                     /translation="MKQVRLSVPKIDSSGLAMGKAAYVDDLAPQNTLIVKILRSPYSY
                     AKVQKIDTSVAENVEGVVCVLTKKDFPRKIFTRAGQGEPEPSPYDKFALDDYVRYIGD
                     EVAVVAAHDEKIAETALKLIKVEYEKFESINDFEKAYESKVVIHPEEEAHPTFDIGYD
                     AKKNLAASYSMSTDDVEKELSRSQIIVHSTYYTQAQAHVMMEPHACATYFDYQGRLTV
                     LSTTQTPFHVRRILSRVLEYPMHKIRVIKPRIGGGFGGKQAIHGEVYCAAVTIKTGLP
                     AKLVYTRREVFQATYTRHPMRIDVTIGAGKNGIINAIDFKILSDTGAYGEHALTVLMV
                     AGSKVLPLYNKASAVSFDGKVVYTNNTPAGAYRGYGAVQGNFALESAVDELAQKLGMD
                     PVEIRKINMIKQGETSPIFRIMGEGREGTDTIINSCKLDYCISRGMELIRWKEKYPCR
                     KISANKVRSVGMAIAMQGSAIANYDMASAAIKLNDDGFFNLTVGATDLGTGSDTILAQ
                     IAADELEVPTEKIIVYSSDTDLTPFDKGAYASSTTYASGNAVKKAAEKIKESIMAKAA
                     EYFKCGISQIKYCEGVVYGPDNRQITLKELSTIIYYSGSQEQIYSCASYVPTVSPPPY
                     MAGFVEVETDLLTGKVEVIEYVAVVDCGTPINPNLAKVQVEGALVQGIGMALYEQVIK
                     SRSGKLLSDSMMTYHIPSRKDIGKITVEFAESYEPSGPFGAKSVGEIGIDTPPAAIAN
                     AIFNGTGKRIRKLPITPEDIIFFKEKHE"
     gene            complement(2831..3283)
                     /locus_tag="PLU28_08955"
     CDS             complement(2831..3283)
                     /locus_tag="PLU28_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOG35226.1"
                     /translation="MIIKINLNNISKELLISPDDTLLDVLRKTGCLSVKKGCESSSCG
                     VCTVLLDGTPILSCSYLAAKADGHSVTTVEGIAEQAREIGEFIAAQGADQCGFCLPSL
                     IISTYYLKNTIGNPTLKDIKQVLQANLCRCSGYESQEAAVARYLGVKI"
     gene            complement(3280..>4019)
                     /locus_tag="PLU28_08960"
     CDS             complement(3280..>4019)
                     /locus_tag="PLU28_08960"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HOG35227.1"
                     /translation="LKESPNNEIIAGALFIRLASKEINTAIDLSECGLRFINEHKDRV
                     EVGAMTSYLDIIQSDCFDTPVLKTVKDALSEIMGIAFQNMATVGGTIYPAYPFSDLIT
                     VLMPLNCWVKLYKKDPVRLSEFIEKKQDGREILEKIIILKGSGKNAFRSFRKTSQDFS
                     VLNLSVAVEHGDCKIAVGARPSVAQYAKSAMQFISGGKVNESIIEKTAEIACQEISFF
                     SDIKASQQYRKHICNVLLKDALREVFV"
BASE COUNT         1070 a          884 c          748 g         1317 t
ORIGIN      
        1 ctaaaactgg gcagcgttat tgaaaaagct tttggaaaaa aagtttttaa tattgatatt
       61 tatctttcag agtttaaaaa agaatttggt gtaaaaccgc ataatgcaag aaactttgat
      121 tttgtaagtt tttaccagat tgttctgctg aaggatgttc ttacacagaa gacagagcag
      181 ttgatgctgg aatatattct gaacaaggat aacgggatat attatatata caataaaaaa
      241 ctcagccagc ttccggccga gtttaaatct ttgcaaactg taagatatat ggatgcagta
      301 gagctcatcg cagaatatcg ttactcttca gactgtcttg cttttgtatc tgattggctt
      361 aagcatcatg ttgaagaaga tggttcctgg gatttgggag caaaggcaaa agataatatc
      421 agctttccga tatctgattc gtggagaagt ccaaactcca gaaaaaaaga ttgtactgcg
      481 agagttacta gactcttacg catgattcaa aaatagatta gtactcatta ttcgtgtttt
      541 tctttaaaaa atataatatc ctcaggcgtt ataggaagtt ttcgtatccg cttgcctgtc
      601 ccattaaata tggcgttggc tattgcagcc ggtggtgtgt ctattcctat ctctcctaca
      661 gattttgcgc cgaaaggacc ggaaggctca taactctctg caaattctac tgttattttt
      721 cctatatctt tgcgtgatgg aatatgataa gtcatcatac tgtctgaaag caactttccg
      781 gatcggcttt ttatgacttg ctcatacagc gccattccta ttccctgtac aagagcccct
      841 tctacctgta ccttggcaag gtttggattt ataggtgtcc cgcagtctac aaccgctaca
      901 tactctataa cttcaacctt tccggtaaga agatctgttt ctacctcaac aaatcctgcc
      961 atatacggcg gaggagaaac ggttggaaca tagcttgcgc aggagtatat ctgttcctga
     1021 ctgccgctgt agtatattat tgtagaaagt tccttcagcg ttatctgacg gttgtctggt
     1081 ccataaacga caccttcgca atacttaatc tgagatatac cgcacttgaa atattcagcc
     1141 gcctttgcca taatactttc cttaatcttt tctgctgctt tttttacagc attaccagaa
     1201 gcatacgtag tgcttgaagc atatgctcct ttatcaaaag gggtaaggtc ggtatctgaa
     1261 gagtacacta taattttttc agttggtact tctaactcat ctgccgctat ctgagccagg
     1321 atggtatcac ttcccgtacc aagatctgtt gctccgacag ttaggttaaa aaaaccgtcg
     1381 tcattaagct ttatggctgc acttgccata tcgtagtttg ctattgccga gccttgcata
     1441 gctatagcca ttccgacact tcggactttg tttgcagata tctttctgca tggatacttt
     1501 tctttccatc ttatcagctc cataccacgg cttatacaat agtccaactt acatgaattt
     1561 ataatggtgt cagttccttc tctgccttct cccataattc tgaatatagg agaagtttct
     1621 ccctgtttta tcatattgat ttttcttatt tctacaggat ccatacctag tttttgtgca
     1681 agctcgtcaa ccgcagattc gagggcaaaa tttccctgaa ctgctccgta tcctctgtaa
     1741 gcaccagccg gtgtattgtt agtataaaca acttttccgt caaagcttac agcactggct
     1801 ttgttataaa gaggcagtac tttggaaccg gctaccataa gcacggtaag tgcgtgctca
     1861 ccgtaagctc ctgtatctga aaggatcttg aaatcaattg catttattat accgtttttt
     1921 ccggcaccta tagtcacatc tatcctcatg ggatgccttg tatatgttgc ctggaaaact
     1981 tctcttctgg tatataccag cttggctggc aatcctgttt ttatggttac agccgcacag
     2041 tagacctctc cgtgtattgc ctgcttgcct ccaaatcctc cgcctatacg gggttttatg
     2101 acccttatct tgtgcattgg atattccagg actctgctga gtattctccg cacatgaaag
     2161 ggagtctggg tagtactgag aacagtaagc cttccctgat agtcaaagta cgtcgcacag
     2221 gcatgcggtt ccatcatgac atgagcctga gcctgtgtat aatatgtcga gtgcactatt
     2281 atctgtgaac gggaaagctc tttttcaaca tcatctgtac tcatgctgta agaagcagca
     2341 agattttttt tggcatcgta accgatatca aaagtaggat gcgcttcttc ttccggatga
     2401 ataacaactt tgctttcata tgctttttca aaatcattta tactctcaaa cttttcatac
     2461 tctactttta ttagttttaa cgctgtttct gcaatttttt catcatgtgc tgccacaact
     2521 gctacttcat caccgatata gcgtacataa tcatcgagtg caaacttatc atatggagag
     2581 ggttcaggct ctccctgacc tgctcttgtg aatatctttc ttggaaaatc ttttttcgtc
     2641 agaacacaga caactccttc aacgttctca gctaccgaag tatctatttt ttgtactttg
     2701 gcatagctgt aaggacttct cagaatcttt actataagag tattttgggg tgcaagatca
     2761 tcaacatagg cagcttttcc catagcaagt cccgatgaat caattttcgg aacagaaagc
     2821 ctgacctgct tcatattttc acccccagat atctggcaac tgctgcctcc tggctttcat
     2881 accctgaaca tctgcacagg ttagcctgga gaacctgttt tatatcctta agcgttggat
     2941 ttccaatcgt atttttcagg taatacgtgc ttattattaa agatggaaga cagaaaccac
     3001 attgatcggc accctgcgcc gcaataaact ctcctatttc tctggcttgc tcagcaattc
     3061 cttccaccgt tgtaacactg tgaccgtcag ctttggcagc taaatatgaa catgacagta
     3121 tgggcgttcc gtcaagcagg actgtgcata caccgcagct tgagctttca caaccttttt
     3181 ttacactcag gcaaccggtt ttcctaagaa catccaaaag agtatcgtcc ggacttataa
     3241 gcagttcttt tgaaatgttg ttcaaattta tttttattat catacaaaaa cctccctgag
     3301 agcatctttc agaaggacgt tgcagatatg ctttctgtac tgttgactgg cttttatatc
     3361 agaaaaaaac gaaatctcct ggcatgcgat ctccgcggtt ttttcaatta tgctttcatt
     3421 tacttttccg cccgaaataa actgcatggc actcttggca tactgtgcaa ccgaaggtct
     3481 ggcacctacg gctatcttgc aatctccatg ttcaacggct acactgagat taagcaccga
     3541 aaagtcctgg ctggtctttc tgaatgatct gaaagcattt ttccctgagc cttttagtat
     3601 tattattttt tcaagtattt cccggccatc ctgttttttt tcaatgaact cagaaagtct
     3661 taccggatct tttttataaa gttttaccca gcagtttaac ggcatgagaa ccgttataag
     3721 atcagaaaaa ggatatgccg gatagatagt tcctccgacg gtagccatat tttgaaatgc
     3781 tatgcccata atttcactta gagcatcttt cacagttttt aacacaggag tgtcaaaaca
     3841 atcagactgt attatatcca ggtatgatgt catggctcca acttccaccc tgtctttatg
     3901 ctcattaata aatcttagtc cgcattctga aagatctata gcagtattta tttctttaga
     3961 ggcaagtctt ataaaaagag ctcctgcgat tatttcatta tttggagatt ctttgagag
//
LOCUS       DAOSHR010000181         4003 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_20485_length_4003_cov_7.094478, whole genome shotgun sequence.
ACCESSION   DAOSHR010000181 DAOSHR010000000
VERSION     DAOSHR010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 4003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4003
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20485_length_4003_cov_7.094478"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="PLU28_08965"
     CDS             <1..607
                     /locus_tag="PLU28_08965"
                     /inference="COORDINATES: protein motif:HMM:NF019940.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1732 domain-containing protein"
                     /protein_id="HOG35228.1"
                     /translation="KIKTDLSMNDDITLDILTRFKEIIRINLDEQSMDEVWSGLSQCL
                     NDVVVEVKKFQNREGLNLKLLLADYIKQLSDIVENIYAKSLEMKEVFMQKLTEDLNQL
                     LKPDTKINPDRLELEVAMLAERSDISEEIERLKIHLKRFREILESPEELKGQNLDFLS
                     QEMHREFNTIASKSKIAEITNCSIDGRVIVNKVREQVQNIH"
     gene            629..922
                     /locus_tag="PLU28_08970"
     CDS             629..922
                     /locus_tag="PLU28_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF370 domain-containing protein"
                     /protein_id="HOG35229.1"
                     /translation="MYGLINIGFGNVVVGDRVIAIVNPGSQPLKRLRDVAEQQGKLLE
                     VNHGRKTRAFIITDSGHVIASAIQPETITSRFFQSFYDIEKALERIRKEAFNE"
     gene            915..1547
                     /gene="gmk"
                     /locus_tag="PLU28_08975"
     CDS             915..1547
                     /gene="gmk"
                     /locus_tag="PLU28_08975"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOG35230.1"
                     /translation="MNDETKNGILYVVSGPSGAGKSSIIKRALQSVQGFSYSVSFTTR
                     PARPDETDGIDYFFVDNTGFDAIIEKNDFLEWANVHGYRYGTSKSHVEKKLDEGYNII
                     LDVDVQGAVNIKNVLPERTVTIFVAPPSYDDLSRRLSQRATENERDLKKRLEDAKAEL
                     AKIPYFDYLIVNSVLAESISQLISIIIAEQLKVKRVAEHLGQYTFFRNNP"
     gene            1564..1800
                     /gene="rpoZ"
                     /locus_tag="PLU28_08980"
     CDS             1564..1800
                     /gene="rpoZ"
                     /locus_tag="PLU28_08980"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HOG35231.1"
                     /translation="MKTNVNYDKIMKEVNFKYAVPIIAAKRAEILKDSEEMQNSTIKT
                     QDGNYVTIALREIELGIIKVKDIDKLNSLRTEMK"
     gene            1797..2369
                     /locus_tag="PLU28_08985"
     CDS             1797..2369
                     /locus_tag="PLU28_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35232.1"
                     /translation="MNSLRRGLIILFSVLLISTGLFAGTFYDLGFSVLNDVSPFKVAI
                     RGGIESPNINFSFDINPNLTPLSLVSVTDINALLYGFNETTSIHGGMIWYYDRATLPA
                     TDTVSPNRSQPDLYLGISYKLDFLKADLNFCYPLVKDFKPSQNILDYFMIKTVFTFPR
                     PHESFIDDLKFELRFSSLRRDFSIYISEPF"
     gene            2369..2824
                     /locus_tag="PLU28_08990"
     CDS             2369..2824
                     /locus_tag="PLU28_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35233.1"
                     /translation="MKSIKEGFILLQSVFEIFFVFMGTMTVLACFARTVFVLKQSLDY
                     MLQNGIMANSVKTVILICKNEVERVDVKLETPFYIRLYRQDGFSRIGFYYNKNQHKLY
                     RYSKNDTSSTGETYIADNIDMRYEDRFLKVSFGNSPEICLYLPDNTTED"
     gene            2826..>4003
                     /locus_tag="PLU28_08995"
     CDS             2826..>4003
                     /locus_tag="PLU28_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOG35234.1"
                     /translation="MANNIIESYKDLQEKIKFENSQIFLGMLESPYPAFRSKAINELA
                     KMKTETPIIKDFLDDSDFSVRFSALKYLEKMGKLELEQTEKLLTDINAVIRKEAVRLY
                     IEQDFEPTERVFVCCSDPDPSVRYQLFTSYIQTFPDETSVIVEKMRNDPYIKIQQLIK
                     ALDDISGTLLSPDADSNVKRAALSRYYESSDSITFFNTIKEIYPKSDRQTKTIIIRFF
                     SGLPGDIIKGFLEQKIEEEKDYSLLLELAKVARKACGKDFVPSWMVDKLIKSSDSIMI
                     SYGLKLAADKDDMTYVSFCRELLNSIEDSLVIACINYLVYFQDYSLSERIPDFIQSLS
                     SKRISAALKVVKKLKLDQYTYEIAAIAKEKKFPVSIRKNALNILRYFKVKEMWDVPYN
                     "
BASE COUNT         1335 a          734 c          781 g         1153 t
ORIGIN      
        1 aaaaataaag actgatcttt ctatgaatga tgatattacc ttggatattc ttacaagatt
       61 taaggagata ataagaataa atctcgacga gcagtccatg gatgaagtgt ggtcagggct
      121 aagccagtgt ctcaatgatg tggttgtgga ggtaaaaaaa ttccagaaca gagaaggctt
      181 aaaccttaaa ttactgcttg ctgattatat aaaacagctt tccgatatag ttgaaaatat
      241 ttatgccaaa tcacttgaaa tgaaggaagt atttatgcaa aagctgaccg aagaccttaa
      301 ccagctcctt aaaccagaca ccaaaataaa cccagaccgt ctggagcttg aagttgcaat
      361 gctggctgaa cgctctgata tctctgaaga aatagaacga ttgaaaattc atctcaaacg
      421 cttcagagaa atcctggaat cccctgaaga acttaaggga cagaatcttg actttttgtc
      481 gcaggaaatg cacagagagt tcaatactat agcttcgaaa tccaaaattg cagaaataac
      541 taattgctcg atcgatggac gggtgattgt taacaaagta cgggagcagg tacagaatat
      601 ccattaacaa atttttggag gtgcttatat gtacggtctt ataaacattg gtttcgggaa
      661 tgttgtggtt ggagatagag ttattgcaat cgttaatcct ggttcccagc cgcttaaaag
      721 acttagggat gttgcagaac agcagggaaa acttttggaa gtgaaccacg gcagaaaaac
      781 aagggcattt attataaccg actcaggtca cgttatagcc agtgctattc agcctgaaac
      841 cattaccagc cgttttttcc agagtttcta cgatatagaa aaggctttgg aaagaatacg
      901 aaaagaggct ttcaatgaat gatgaaacaa aaaacggtat tctttatgta gtcagcgggc
      961 cttcaggtgc cggaaaatct tctatcataa aaagggcttt gcaatctgtt caaggtttct
     1021 cttactctgt atcgttcaca acaaggcctg cgcgtccgga cgaaactgac gggatagatt
     1081 atttttttgt tgacaatacc ggatttgatg cgatcataga aaagaacgat tttcttgaat
     1141 gggcaaatgt gcacggttat agatacggaa cttcaaaaag ccacgtcgaa aaaaagcttg
     1201 atgaaggcta caacataatt cttgatgttg acgttcaagg agctgtaaat attaaaaatg
     1261 ttttgcctga aagaacagta accattttcg tcgcacctcc ttcttacgat gatctcagca
     1321 gaaggctttc tcagcgggct acagaaaacg agcgtgatct gaaaaaaaga ctggaggatg
     1381 ccaaagctga attagctaaa attccttact ttgattacct catagttaat tcggttctcg
     1441 ctgaatctat aagccagctg atttcaatta taatagctga gcagcttaaa gttaaaagag
     1501 tcgctgaaca tctcggacag tacacatttt tcagaaataa tccctgaatc ttcggaggta
     1561 aaaatgaaaa caaatgtaaa ctacgacaag ataatgaaag aagtgaactt caagtatgcc
     1621 gttcccataa ttgcagctaa gagagctgaa attctaaagg acagcgaaga aatgcagaac
     1681 tccactatta aaacccaaga cggaaactat gtcactatag ctctgagaga aatagagctt
     1741 ggaataataa aagtaaagga tatcgataag cttaactctc taaggactga aatgaaatga
     1801 actccctgcg cagaggcctg atcatattat tctctgtttt actgatttct acgggacttt
     1861 ttgcgggaac cttttatgat cttggatttt ctgttctcaa cgacgtttcg ccgtttaagg
     1921 ttgctataag aggcgggata gaatctccaa acatcaattt cagctttgat ataaacccta
     1981 atctgactcc actgagcctt gtttccgtta cggacataaa tgctcttctt tatggtttta
     2041 acgaaaccac aagtattcac ggcggaatga tctggtacta tgacagggct actcttcctg
     2101 ccacagatac ggtttctcca aacaggagcc aaccggatct atatcttgga atatcttata
     2161 agctcgattt tttaaaggca gatcttaatt tttgttatcc gcttgtaaag gattttaaac
     2221 ccagtcagaa catattggac tactttatga taaagactgt attcactttt cccagaccac
     2281 acgaatcctt tatcgacgat ttgaagtttg aattacggtt cagcagtctc agacgcgatt
     2341 tttcaatata tatttccgaa ccgttctgat gaaaagcatc aaggaagggt ttattctttt
     2401 acagagcgtt tttgagatat ttttcgtttt tatgggaaca atgacagtcc ttgcttgttt
     2461 tgcaaggact gtgtttgtgt taaagcaatc gcttgattat atgcttcaga acggaataat
     2521 ggctaattca gttaaaaccg tcatccttat atgcaaaaac gaggttgaac gagtagatgt
     2581 aaagcttgaa acgccttttt atataaggct ttaccgtcag gatggtttca gccgcatcgg
     2641 cttttactac aataagaacc aacataaact ttacagatat tctaaaaacg atacttcttc
     2701 aacaggagaa acttatattg cagacaatat tgacatgaga tacgaggacc gatttttaaa
     2761 ggtttctttc ggcaacagtc cagagatatg cttatactta ccggacaaca ctacggagga
     2821 ttaatatggc aaataatatt attgaatctt ataaagatct tcaggaaaaa ataaagtttg
     2881 aaaattcaca gatttttctc ggtatgctcg aatctcctta tcctgctttc agatccaaag
     2941 ccataaatga acttgcaaaa atgaagaccg aaactcctat aataaaggat tttttagatg
     3001 acagtgattt ttctgttaga ttttcagctc tgaagtatct tgaaaagatg ggaaaactgg
     3061 aattggagca aacagaaaag ctattgactg atattaatgc cgtgatccga aaggaagctg
     3121 tcagactcta catagaacaa gattttgaac ctacagagcg tgtctttgta tgctgcagcg
     3181 atcctgatcc gtctgtcagg tatcagcttt ttacatctta tatacaaact tttccggacg
     3241 aaacttctgt tatagttgag aaaatgagaa acgatccgta cattaaaatt caacagctca
     3301 taaaagcact ggacgatatt tcaggaacac ttctatctcc agatgctgat tcaaatgtaa
     3361 aaagagctgc tctttcaaga tattacgaaa gttcagattc aataactttt tttaacacca
     3421 taaaggaaat ataccccaaa tctgacagac agaccaaaac catcattata cgtttctttt
     3481 ccggactacc tggagacata ataaaaggat ttcttgaaca gaaaattgaa gaagaaaaag
     3541 attactcact tctgcttgaa cttgcaaaag ttgccagaaa agcctgcgga aaagattttg
     3601 tgccgtcatg gatggtcgat aagctcataa aaagctctga cagtataatg ataagctatg
     3661 ggcttaagct tgcagcagat aaagatgaca tgacctatgt gtccttttgt cgtgagcttt
     3721 taaacagcat tgaggattcg cttgtaattg cctgcataaa ctatctggtt tactttcagg
     3781 actattcact ttcggaaaga attcctgact ttatacagag cctatcctcc aaaagaataa
     3841 gcgccgcact taaggtggta aaaaaactga agctagatca gtacacatac gaaatagctg
     3901 ctattgccaa agaaaaaaaa ttcccagtat ccatacgtaa aaacgcattg aatattcttc
     3961 gttactttaa agtcaaggag atgtgggatg taccttataa tat
//
LOCUS       DAOSHR010000182         3965 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_20795_length_3965_cov_5.661893, whole genome shotgun sequence.
ACCESSION   DAOSHR010000182 DAOSHR010000000
VERSION     DAOSHR010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 3965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3965
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20795_length_3965_cov_5.661893"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..853
                     /locus_tag="PLU28_09000"
     CDS             <1..853
                     /locus_tag="PLU28_09000"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HOG35235.1"
                     /translation="LKIKAIDKKLSPSNIDIDFGEVYDTIMEKNFQFHEISSLKETLD
                     ITIKEFLKLFENLAASNEEIKASNEELENLYKELEESYNQVESSYWKFSKQLSNIAEK
                     YDDVTGNHIDRVAEYALFVSEKMGLSKKVQEDLYYFSPLHDIGKLLVKREILDKPGKL
                     TSAEFEEMKKHTIYAAEILGDDKQFSIAKNIALYHHERFDGTGYPFGLKGSDIPIEAR
                     IVSICDVYDALRSKRPYKKGFTHEQACEIILCGDEKTSPSQFDPAVLKIFKENHTVFK
                     EIFDRQN"
     gene            922..2334
                     /locus_tag="PLU28_09005"
     CDS             922..2334
                     /locus_tag="PLU28_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HOG35236.1"
                     /translation="MADKVIVFSKSNEEIKVALIEDEILSEIFFEDVETEKNSGKIFI
                     GRIENDVPSLEAFFVNIGINKNGFLRYKDLIGLPNEYKRGDKVIVQVKKDGTIRKGPQ
                     LSMQINLPGRYIVYLPEGESSIGISRKITDDLTRQDLRELGEDIINEREGIIFRTNCT
                     SIDKKTIREEFESLRESWKNIVASYKSTQKPILMYSDQNFVEYILRERLDKDTRKIIL
                     DDKSVNETVEQVLKEMNYSINVELTDRDAFSYMNIYGDMNDIFARKITLKNGAIITID
                     RAEAMTVIDVDSASNVQEKNIEETSFKTNLSAAKEIARQLRLRNISGIIIIDFIDMMD
                     RQHQEAVKTFLEQETYRDKAKVSVVGFTSLGLLELTRKRTSSSIESYMFSPCPVCHGT
                     GRVASPRVVFDRMIKDIHDSMRLFEDDNIAEVMLNVYHNLSGYITPEIKSCLEDKYGI
                     KLKFEFSWNDPNSYNIRFRK"
     gene            2348..3592
                     /locus_tag="PLU28_09010"
     CDS             2348..3592
                     /locus_tag="PLU28_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35237.1"
                     /translation="MKADKTLITLYGLLAFFSLVFILSVAFLYKSDPVEKLIPHSSSR
                     EFIEYISSGEKTEKLLGSTFIKLFDVKGGEFLKICASEKNSYGVLMKSYDGYRIYTID
                     SRGAVLYSFAAERSSAKINDIKYKDGLYLLAGEKEGFAYIGAYGAADGQLRWSNFLNL
                     KGEFKTLRPVSQGYLAGGYLINAQSKQALTTELNLNGDTVWTDTYTRGDNEYISSIVP
                     LADGFFAIGSSNSNPLKDFNVLILRYDIHGIKRFDGALIIEHHDETPLSAVSDKEENL
                     FICGLRTDASSYAQKSLILKLPFPKDDTDRLKETFLDIKAIDKQSSLNSIIFHNNRAY
                     CVGSISADDGKLCGFLKIINSDGSLYYERVYDINEKSWFSDIIPTQENTLLIVGAVVK
                     NGVKTPAVFSTDLKGGISETPE"
     gene            3677..3749
                     /locus_tag="PLU28_09015"
     tRNA            3677..3749
                     /locus_tag="PLU28_09015"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3710..3712,aa:Lys,seq:ttt)
     gene            3812..3901
                     /locus_tag="PLU28_09020"
     tRNA            3812..3901
                     /locus_tag="PLU28_09020"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3846..3848,aa:Leu,seq:tag)
BASE COUNT         1322 a          753 c          816 g         1074 t
ORIGIN      
        1 tctaaaaatc aaggcaattg ataaaaaact ttctccatca aatatagata ttgattttgg
       61 agaagtttat gacaccataa tggaaaagaa cttccagttt catgaaatct caagcctcaa
      121 ggaaaccctt gatattacaa taaaagagtt tttaaagctt tttgagaatc ttgctgcttc
      181 aaacgaagag ataaaagcct ccaacgaaga gcttgaaaat ctttataagg agcttgagga
      241 aagctacaac caagttgaaa gctcttattg gaagttctca aagcagcttt caaatatagc
      301 cgagaaatac gatgatgtaa ccggaaatca tatagaccgt gtggccgaat acgcactgtt
      361 tgtttctgaa aaaatgggat tatcaaaaaa agttcaggaa gatctctact acttttctcc
      421 tctgcacgat ataggcaagc ttctggtgaa aagagagata cttgacaaac ccggaaaact
      481 taccagtgcg gagtttgaag agatgaagaa acatactatc tacgccgcgg aaattctggg
      541 tgatgacaag cagttctcta tagccaaaaa tatagctctt taccaccatg aaagatttga
      601 cggtaccgga tatcctttcg gacttaaagg ttcggatatt cccatagaag cacgtatagt
      661 cagcatatgt gacgtctacg atgcattacg aagcaaaagg ccttacaaaa agggctttac
      721 tcatgagcag gcctgtgaga ttatactttg cggtgatgaa aaaacatcgc cttcacagtt
      781 tgatcctgcc gtactcaaga tattcaaaga aaatcatact gttttcaaag aaatttttga
      841 caggcaaaac taaatacccg gtaaaacggg tgttttttta tgatagaatt gtcttatact
      901 acatacttaa aggggtttaa aatggcggac aaagttattg tattcagcaa atccaacgaa
      961 gagattaaag ttgccttaat agaagacgag atactttctg aaattttttt tgaagatgtt
     1021 gaaaccgaaa aaaattccgg aaagattttt ataggacgta tcgaaaacga tgttccgagt
     1081 ttggaagctt tctttgtaaa cataggcata aataaaaacg gttttctcag gtacaaggat
     1141 cttattggcc ttccaaacga gtataaacgc ggtgataagg tcatcgtaca ggtaaaaaag
     1201 gacggaacca tacgcaaggg accacagctg tcgatgcaga taaatcttcc aggcagatat
     1261 attgtatatc ttccggaagg agagtccagt ataggcattt cacgcaagat aaccgatgat
     1321 ctcacacgtc aagatttaag agagcttgga gaagatataa taaatgagcg cgaaggaata
     1381 attttcagaa ccaactgtac ctccatagat aaaaaaacca tacgtgaaga gtttgaatca
     1441 ctcagagaaa gctggaaaaa catcgttgct tcttataaat ccactcaaaa acctattctg
     1501 atgtattctg accaaaactt tgtggagtat atactgcgag aacggctgga caaagacaca
     1561 cgaaaaataa ttcttgatga taaatccgtt aatgaaaccg tagagcaggt cctaaaagag
     1621 atgaactact ccataaatgt cgaacttacg gatcgtgatg ccttttcata catgaacata
     1681 tatggcgata tgaacgatat atttgcacgg aagataactc ttaaaaacgg tgccatcata
     1741 acgatagaca gagcagaagc tatgactgtt atcgatgttg actccgcaag caacgttcag
     1801 gaaaaaaata ttgaagaaac ttcatttaaa accaatcttt cagctgccaa agaaatagcc
     1861 aggcagctgc ggttaaggaa catatcagga ataatcataa tagacttcat agatatgatg
     1921 gacagacagc atcaggaggc agttaaaact tttttggaac aggagaccta cagagacaag
     1981 gcaaaagtgt ctgttgtagg tttcacaagt cttggactgc ttgaacttac aaggaaacgc
     2041 acttcttcat caattgaatc gtatatgttc tctccctgcc cggtgtgtca tggcacaggc
     2101 agagttgctt cacccagagt tgtatttgac aggatgataa aagatattca tgactcaatg
     2161 agactgtttg aggatgacaa catagcagag gtaatgctca atgtctatca taatctttca
     2221 gggtacataa ctcccgaaat taaaagctgc cttgaagata agtacggtat caagctcaaa
     2281 tttgaattca gttggaatga tccgaactcc tacaacataa gattcagaaa ataaggggga
     2341 caatactttg aaagcagata aaacccttat aaccctttac ggactgttag cctttttttc
     2401 gctggtcttt attctttcgg tcgcttttct ttacaagagt gaccctgttg aaaaactcat
     2461 tcctcacagc tcgtctcgag agtttataga gtacatttcg tcaggagaaa agactgaaaa
     2521 gctccttggg agtaccttta taaaactttt tgacgtcaaa ggaggagaat ttctgaaaat
     2581 atgtgcctca gaaaagaact cctacggcgt gctcatgaaa tcatatgacg gatacagaat
     2641 atatactata gacagcaggg gcgctgtgct ttattctttc gccgctgaaa gatcttcagc
     2701 caaaatcaac gatataaagt ataaagacgg tttatacctt cttgcaggag aaaaagaagg
     2761 atttgcatat atcggtgcat atggtgcagc agacggacag ctcagatggt caaactttct
     2821 gaatcttaag ggcgagttca aaactctcag acctgtctca cagggctacc ttgcaggagg
     2881 ctatttaata aacgcacaat ctaaacaggc tttgaccacc gaactgaatc tgaacggcga
     2941 tacagtatgg acagatacct acaccagggg tgataacgaa tacatatcct ctattgtccc
     3001 gctcgcagac ggattttttg ccataggctc ctctaattca aatccactga aggatttcaa
     3061 tgttttgata ctacgatatg atatccatgg aattaagaga tttgatggcg cacttatcat
     3121 tgaacatcat gatgaaactc ctttatccgc tgtttcagat aaagaggaaa atctttttat
     3181 atgcggattg agaaccgatg cttcttctta tgcccaaaaa agtctcatac taaagctccc
     3241 ttttccaaaa gatgacacag ataggcttaa agaaacattt ttggacataa aagccataga
     3301 taaacagtct agtctgaaca gcataatatt tcataacaac cgtgcttact gtgtcggtag
     3361 catatccgca gatgacggaa aactgtgcgg atttctgaaa ataatcaact ccgatggttc
     3421 gctttactat gaacgtgtgt atgatatcaa tgagaaaagc tggttttcag atatcatccc
     3481 gacgcaggaa aatactctgc tgatagtggg agcagttgta aaaaacggag taaaaactcc
     3541 cgctgttttc agtacagatc ttaaaggcgg aataagtgaa acacccgagt gaacaactgt
     3601 taagatttcc gtatataaac tttaacttga ctattttcca tacatgaggt ataatatttt
     3661 tcagttatgt agcagtgacc tgctagctca gctggtagag catttgactt ttaatcaaag
     3721 ggccctgggt ccgaatccca ggcgggtcac tattttttgt tgcaaacacg tctactctgt
     3781 tttgcaacat tttgtttttt gaagcaaacc ggcgtgagtg gcggaattgg cagacgcgct
     3841 ggacttagaa tccagtggac cttataagcc cgtgtgggtt caagtcccac ctcgcgcacc
     3901 ataataaaaa tgcggatgta gctcagtggt agagcacctg cttgccaagc agggggtcgc
     3961 gggtt
//
LOCUS       DAOSHR010000183         3902 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_21272_length_3902_cov_4.865870, whole genome shotgun sequence.
ACCESSION   DAOSHR010000183 DAOSHR010000000
VERSION     DAOSHR010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 3902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3902
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21272_length_3902_cov_4.865870"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            82..414
                     /locus_tag="PLU28_09025"
     CDS             82..414
                     /locus_tag="PLU28_09025"
                     /inference="COORDINATES: protein motif:HMM:NF015602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlaG"
                     /protein_id="HOG35238.1"
                     /translation="MDINHVASKPLFDTQKTIQAPVEKKNEKNTEGQQLSLDNVKDIL
                     QKKMENVKLLFNGEAKFEIDREADLIVVKIIDKESKELIRQIPAEEAVKLAKAMNEIE
                     GIIFDQKA"
     gene            complement(440..2125)
                     /locus_tag="PLU28_09030"
     CDS             complement(440..2125)
                     /locus_tag="PLU28_09030"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOG35239.1"
                     /translation="MDLKVYRWVRTVFFAIAATVTLIIFPVHFIFDNFFPIKLLIVTV
                     LTFFSDNLPIYWKKDRKVVSNAIGFLAAYLFGKEYILAAAVTTTFRINFKQDASVIIY
                     RFLAYFMMYYPAAIISGFFSNKIFSAIIYLHLVIIFNIIIVNIIGRRRIDKSSTLIEY
                     LLFMSLLPAIVVFYNTTSILINVMIFIMEIIVLFFFFFSSKYFYERREEKMKNDRLKK
                     YNTVVFDFSQILKALALKIEVEEILDKVADICKNQLGFQKVLISLFNLQDGKVRRITH
                     KGLTDEQYEQIKNREVAIEEIRAFMDEKNRYLETYFIPSEQIDPANQVTFKFDETKKV
                     YEDDGLSHESWTADDMLLLSFRNSNDRIIGYASLDSPVNGLRPTQEDLTILSVFAQTI
                     SLTLVNSQVYNQALDVAFKDGLTGLYNHSKLFEDLSVNWGKGVLASIAFMDLDNFKTV
                     NDTKGHVYGDKILKSIADLFMIHVRIQDKVYRYGGDEFVIIFSGVDKSSCRVVLERLL
                     VKMKESINENISFSVGITDSNERPDYREALNLADRRSYISKFTGKGKIISEEK"
     gene            complement(2190..3083)
                     /locus_tag="PLU28_09035"
     CDS             complement(2190..3083)
                     /locus_tag="PLU28_09035"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HOG35240.1"
                     /translation="MLNGKVGLAPMADYSDYPFRKICSKFGAEFTFTEMISSEGIVRN
                     NSRTYEMLPQDDEKNIGVQIFGSSISVMVESARKVQKYGDWIDINAGCPVTKVVRRGA
                     GSALLLYPEKLAYMIREIRKSVSKPLGVKVRIGYEKNNIQQIMESLKKVDLDYIIVHP
                     RTREQLYSGKAYREILSYVRENTQAMLGASGDIFTRQDIDECFNKYKVDFVIVARGAI
                     GNPWIFCDRTPTPDEIKTVLLEHLRMNISFYKIESMAVRMFTKFLVRYVNGMQGAREI
                     RRHLNDLKSYSDVENLLTMLR"
     gene            complement(3129..>3902)
                     /gene="tsaD"
                     /locus_tag="PLU28_09040"
     CDS             complement(3129..>3902)
                     /gene="tsaD"
                     /locus_tag="PLU28_09040"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOG35241.1"
                     /translation="DIDAIAVTYGPGLVGALLVGLNYAKGLSISLNRPLITVNHLMGH
                     IYANFLAIDGLKPPFIILLVSGGHTMLIKALDYMQFEIIGQSVDDSAGEAFDKVARIM
                     GLDYPGGPVIDKLAREGEEKFSFPRPMYDNGYDFSFSGLKTAVLYFIKDNSGYRKEDV
                     CASFQKSIIETLVHKTIKCAEDSKIRKILIAGGVAANSKLRGEMDKYKNRFDIFYPPM
                     GLCTDNAAMIARAGYEKYKRGLFSDLAVDVVPNLSIDKL"
BASE COUNT         1262 a          806 c          589 g         1245 t
ORIGIN      
        1 tttattatat acttttaaag taacatccat tatcagacga taagaaatcg ttaggaggaa
       61 aaattccagg gagggatctt tatggatatc aatcacgttg cttcaaaacc cttgtttgat
      121 acgcagaaaa ccattcaggc accggttgag aaaaaaaatg aaaaaaacac tgaaggacag
      181 cagctgtctt tggataatgt aaaagacatc cttcagaaaa aaatggaaaa cgtcaagctt
      241 cttttcaatg gagaagcaaa gtttgaaatt gacagagaag ccgaccttat tgtagttaag
      301 ataatcgata aggagtcaaa agagcttata cgacagattc cagcagagga agctgttaaa
      361 ctggccaaag caatgaacga gatagaagga attatctttg atcagaaggc ataatgaaaa
      421 ctccccatat ggggagcttt catttttctt ctgatattat tttacctttg cctgtaaatt
      481 tagatatata agatctcctg tcagcgagat tcaatgcttc cctgtaatca ggtctttcgt
      541 tggaatcagt tattccgacg ctgaaagaga tattttcatt tatagactcc ttcattttta
      601 ccagaagcct ttccaataca actctacagc tcgatttatc tactccgctg aaaattatta
      661 cgaactcatc tccaccatat ctgtaaactt tatcctgaat ccttacatgt atcataaaaa
      721 gatctgctat tgattttaat attttatctc catatacatg tcccttagta tcattgactg
      781 ttttgaagtt gtcaagatcc ataaaggcta ttgaagccaa aactcctttt ccccaattaa
      841 ctgagagatc ttcaaaaagc tttgaatggt tataaagtcc agtcaatcca tctttaaaag
      901 ctacatcaag agcctggtta tagacctgtg aatttacaag ggtaagagat atcgtctgtg
      961 caaaaaccga tagaattgta agatcttcct gcgttggacg cagtccgttt acaggggagt
     1021 ccagcgaagc atatcctata attctatcat tagaatttct gaaggaaagc aacagcatat
     1081 catctgctgt ccaactttca tgggatagac catcgtcttc ataaaccttc tttgtttcat
     1141 caaatttgaa agtaacctga tttgccggat caatctgttc tgaaggaatg aagtaagttt
     1201 caagatacct gtttttttca tccataaaag ctcttatctc ttctatagcc acttctctgt
     1261 tttttatctg ctcatactgc tcatcggtaa gccctttatg agtaattctc ctgacctttc
     1321 cgtcctgtaa attaaacaaa cttataagaa ctttttgaaa cccaagctga ttcttacata
     1381 tatcagccac cttatccaga atctcttcta cttcaatttt cagtgccaga gccttaagta
     1441 tctgtgaaaa atcgaaaaca acagtattat attttttaag tctatcattt ttcatctttt
     1501 cctctcttct ttcataaaaa tactttgagg aaaagaagaa aaaaaatagt actattatct
     1561 ccataataaa gatcattaca ttaattagaa tagaagtagt attataaaaa acaacaattg
     1621 ctggcaacaa cgacatgaaa agaagatatt caatcaaagt agaactttta tcaattctac
     1681 gtctgcctat tatgttcact ataataatat tgaagataat tacaagatga agataaataa
     1741 tagctgaaaa aattttgttg ctgaaaaaac cgcttatgat ggcggcagga tagtacatca
     1801 taaagtatgc aagaaatctg tatattatta ctgacgcatc ttgcttgaaa tttattctga
     1861 atgtagttgt aaccgctgct gcgagaatat attccttacc aaaaaggtac gcggctaaaa
     1921 atcctattgc attgctcaca acttttctgt cctttttcca gtatatagga agattatcag
     1981 aaaagaacgt aagcactgtc acaattaaca actttatagg aaaaaaatta tcaaatataa
     2041 agtgcaccgg aaaaattata agagtcacag ttgcagctat agcaaaaaat actgtcctca
     2101 cccaacggta taccttgaga tccattgaag tacccccatt ttgaaaatgc tttattaatt
     2161 aattcttaat atattatatc ataatttttt catctaagca tagtaagaag attttcaaca
     2221 tcagaataac tcttcaggtc gttaagatgt cttctgattt ctcttgctcc ctgcattccg
     2281 ttcacatatc gcacaagaaa tttagtaaac attcgtacag ccatgctttc aattttataa
     2341 aaagagatat tcattctcag atgctccaaa agaacggttt ttatttcatc aggagtcgga
     2401 gtcctgtcac agaaaatcca aggattacca attgctccac gtgcaacaat tacgaaatca
     2461 accttatact tgttaaagca ttcatcaata tcctgcctgg tgaatatatc cccacttgca
     2521 ccaagcatag cctgagtgtt ttctctgacg tacgacaaaa tctctctgta tgctttgcca
     2581 gaatacaact gttctctggt tcttggatga actataatgt aatctaaatc aaccttcttt
     2641 aatgattcca taatctgctg tatattattt ttttcatacc ctattcttac tttcactcct
     2701 aatggtttgg acacactctt tcttatctcc cgtatcatat atgcgagctt ttccggatat
     2761 aaaagcaatg ctgatcctgc accccttcgc actaccttag taacgggaca tcctgcatta
     2821 atgtctatcc aatcaccata tttttgaacc ttcctggcac tttctaccat aacactaatg
     2881 cttgaaccga atatctgtac tccaatattt ttttcatcat cctgcggaag catctcatat
     2941 gttctggaat tattccttac tattccttca gaagatatca tttctgtaaa agtaaactct
     3001 gcaccgaatt tagaacagat cttccggaaa ggatagtccg aatagtctgc cattggggct
     3061 aaaccaactt ttccattaag catgaattct tacaactcct tacaaaaaaa gagcgcataa
     3121 ctctcctttt ataatttatc tatactgaga tttggaacta catcaactgc aagatctgaa
     3181 aaaagacctc ttttatactt ttcatagccc gccctggcta tcatagcagc attgtctgtg
     3241 cataacccca taggcggata aaaaatatca aatctgtttt tatacttatc catctcaccc
     3301 ctgagcttag agtttgctgc aactcctcca gcgataagaa tctttctgat cttagaatct
     3361 tcggcacatt tgatggtttt atgaacaagc gtttctatta tcgatttctg aaatgaagca
     3421 cagacatctt ctttcctata tccgctgtta tccttaataa agtatagaac agccgttttt
     3481 aaacctgaaa aagaaaaatc atatccatta tcgtacatag gacgtggaaa gctgaacttt
     3541 tcttctcctt cccttgcaag cttatctata accggtcctc ccggataatc aagccccatt
     3601 attctggcta ctttatcaaa ggcttctccc gctgaatcat caacactctg acctattatt
     3661 tcaaactgca tgtaatcaag agcctttatc agcatagtat ggcctcctga aaccagcaga
     3721 attataaacg gaggttttaa tccatctatt gccaaaaagt tggcatatat gtgacccata
     3781 agatggttca cagtaatcaa aggtctgtta agacttatgc tcaatccttt tgcatagttg
     3841 agtccgacaa gaagtgcacc aacaagtccc ggaccatatg taacagctat cgcatcaata
     3901 tc
//
LOCUS       DAOSHR010000184         3872 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_21528_length_3872_cov_5.274823, whole genome shotgun sequence.
ACCESSION   DAOSHR010000184 DAOSHR010000000
VERSION     DAOSHR010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 3872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3872
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21528_length_3872_cov_5.274823"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..788)
                     /locus_tag="PLU28_09045"
     CDS             complement(<1..788)
                     /locus_tag="PLU28_09045"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOG35242.1"
                     /translation="MKKISILILSTLIVGLLLTSCTGITKSESATKNEPVTTISYPVT
                     PFEGSIQDGILIVGYNDKNLAMEKLTGIFGKLTIRADIPQLDAFSVNISCSVDQAITM
                     IKKLESEDKEFASSLKYVEPSYKRDLIRPFDKSSIIKDAPGGFTTEYATTTRLTDPHR
                     LWGIYKINADKAWDNSADGENVKVAVVDTGIDGSHPDLSGNFLGGYAPYSDTVLATNT
                     DHSQNAETHGTHVMGTIAALDNDIGVTGVAPKAKVMNIRIFDTDA"
     gene            complement(802..2745)
                     /locus_tag="PLU28_09050"
     CDS             complement(802..2745)
                     /locus_tag="PLU28_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35243.1"
                     /translation="MKKNLIVLFVVILSLALVLTSCMVDKDPEINVTDQSVLEGDTLT
                     VDLSTKVTDPEGKPITITKVSGVGTLTGKVYTYTPSFSDAGVKEVTLKASDGKREVQK
                     TFKITVRNAVAGSVVVRVAEYTTGPGLEDADVKIDKDGTVIGSGKTDKDGNVTIVFKY
                     EGSSDASFPVKISAKKTGYTAPSVITGKCLNVSNSGTPANNGIIDIQARKALTSTETS
                     NLPDLEIKLYDLSKTIVDPTNISTNTILVYVKNKNMLDGKPDTIEVGYVGLGYVPTAT
                     ARNAAFTGTSEATMTISLIGYDKKTTDLNIVAFDLNDARIQYTIPLTVNYGSAPVTLE
                     KYAPKVIGAVGFTSDVDLRYYSTGLISKLSKDLPFDNLNEVFLDARNSDENIFMQVQW
                     TTPATKTGIVGYNLYRSEDKVNWEFIKNRVGNTVTSLTESCFGVVPGKKYYYNVKSLY
                     SDGTESLASNTLEVIPLKSFDVRLNSPANNAIDVGLTPQFSWTPVESQDHSKTVNLSN
                     GLTNDASILFHYTPWIYDTTQDESGIFVIDSFGDQFDFVTAGAQEVTVDFLCSSWGGA
                     STRWVLNSVIYPYKGLEQGKTYEWGHDFAYAQYQSARDENSAYFIWTSVSIDLGFGLN
                     WLNNPSLKNRTEYYNRFTTGNGL"
     gene            complement(2781..3578)
                     /locus_tag="PLU28_09055"
     CDS             complement(2781..3578)
                     /locus_tag="PLU28_09055"
                     /inference="COORDINATES: protein motif:HMM:NF025701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease complex subunit PrcB family protein"
                     /protein_id="HOG35244.1"
                     /translation="MKRMFLIVAVFLLVFTLMFSSDTWQGSAASDFFIQNSFYEKIGE
                     YYFQCLNYELADNFFLSELKSSIDSYRNADFSKKGSLKTFYERVEALYNLSLNASSNV
                     SSRYALIKLNEILILHSDIKTMGENIIGMSDGPTSIYLTDTKTQQPASPVFKYEAMQP
                     DQVYAEIGIIKEKSRGFKILKQEKNLFYVLVSAGEKSTGGYSLNLEDITFKDSTVTIK
                     IKETSPARGKPVTQAFSYPQLVLKFYFDYSAVKNILVTINNVELSNL"
BASE COUNT         1165 a          761 c          687 g         1259 t
ORIGIN      
        1 gcatccgtat caaaaattct tatattcata accttagctt ttggtgccac tccggtaact
       61 cctatatcgt tatcaagtgc agctatggtt cccattacgt gtgtaccatg agtttctgcg
      121 ttctgtgaat gatcagtatt ggttgcaaga actgtatcgg agtatggagc atatccgcca
      181 aggaagttgc cggaaagatc cggatgtgaa ccgtcaatac cggtatctac cacagcgact
      241 tttacatttt ctccgtcagc agagttgtcc catgctttat cggcattgat tttatatatt
      301 ccccaaagtc tatgaggatc tgttaaacga gttgtagttg catattcggt tgtaaaacca
      361 cccggagcat cttttattat agaactctta tcaaatggtc ttattaaatc tcttttgtat
      421 gaaggttcta catacttaag actcgaagca aattccttat cttctgactc aagctttttt
      481 atcatagtaa tggcctgatc aacgctacat gagatattta cactaaaagc atccaactgt
      541 gggatatctg ccctaatagt cagctttcca aaaattccgg tcagcttttc cattgccaga
      601 tttttatcgt tgtagccgac tataagtatt ccatcctgta tcgaaccttc gaaaggagtt
      661 accggatagc ttatggttgt tacaggttca ttttttgttg cactctctga cttagtgata
      721 ccggtacagc ttgtcaaaag caatccgaca atcaatgtac ttaaaattag gattgaaatc
      781 tttttcattc agctcacctc cttataatcc atttccggtt gtaaatctgt tataatactc
      841 ggtgcggttt ttaagactcg gattgtttag ccagttaagt ccgaagccaa gatcgataga
      901 aacacttgtc catataaaat acgcactgtt ttcatctctt gcactctgat actgtgcata
      961 ggcaaagtcg tgaccccatt cataggtctt gccctgttct aatcctttat aaggatatat
     1021 cacagagttt aaaacccatc tggtagatgc tcctccccat gatgaacaca gaaaatctac
     1081 agttacttcc tgtgcgcctg cggtaacaaa atcaaactga tctccaaatg agtcgataac
     1141 aaatattcct gattcatcct gagtggtatc atatatccac ggagtatagt gaaatagtat
     1201 agaagcatcg ttagttagac cgtttgaaag gtttacagtt ttagaatgat cctgcgattc
     1261 aaccggtgtc cagctaaatt gtggagttaa gccgacatct atggcgttgt ttgcaggaga
     1321 gttgagtctt acatcaaagc ttttcaaagg aataacttca agggtattgc ttgctaaaga
     1381 ttctgttccg tcagagtaca gggactttac attgtagtag tattttttac ccgggactac
     1441 tccaaaacag ctttcagtca atgaagtaac agtatttcct actctgtttt ttataaattc
     1501 ccagttaacc ttatcttcgg atctgtagag attatatcct acgatacctg tttttgttgc
     1561 aggcgtagtc cactgaacct gcatgaaaat attttcatca gagtttcttg catcgagaaa
     1621 tacttcattg aggttatcaa acggtaaatc ttttgaaagc ttgcttatca gaccggtaga
     1681 ataataccta agatcgacat ctgatgtaaa accgacggct cctataacct ttggtgcata
     1741 cttctccaaa gtgaccggag ctgagccata attgacggta agcggtatgg tatactgtat
     1801 cctggcatcg ttaagatcaa aggcaacaat gttaagatct gtggtctttt tatcgtatcc
     1861 gataagagaa attgtcatcg tagcttctga agtacctgta aaagctgcat ttctggcagt
     1921 ggcagtggga acatatccca atccgacata acctacttca atagtgtcag gttttccatc
     1981 aagcatattt ttgtttttta catacactaa gatagtattt gtggaaatat tggttggatc
     2041 aacaatagtt ttgcttagat cataaagttt tatttcaaga tctggcaggt tggaagtttc
     2101 tgtgctagtc agagcctttc tggcctgaat gtcaataata ccgttgttgg ctggagttcc
     2161 ggagttgctt acgttaagac atttccctgt aataacgctt ggtgcggtat atccagtctt
     2221 tttagcagat atttttacag ggaatgaagc atcagagctt ccttcgtatt tgaaaacaat
     2281 agtaacattt ccatctttat cagttttccc gctgccgata actgttccgt ctttatctat
     2341 tttgacatcg gcatcttcga gtcctgggcc tgtagtgtat tctgctactc ttacaactac
     2401 cgatcccgca actgcatttc gtacagtgat tttaaaggtt ttctgtactt cccttttgcc
     2461 atcgctggct ttaagcgtta cctctttaac gccggcgtcg ctgaatgaag gggtataagt
     2521 ataaacttta cctgttagag tacccacacc gcttactttg gttattgtaa tcggttttcc
     2581 ttcaggatcc gttaccttgg tagaaagatc taccgtaaga gtgtctcctt caagtactga
     2641 ctgatccgta acatttattt ccggatcttt gtctaccata caagaagtaa gaaccagagc
     2701 aagacttaag ataacaacaa acagtacaat caagttcttc ttcatctttt taatccctcc
     2761 ttgttaagat aataaagttt tcataagttt gaaagctcca cattatttat tgtaacaagt
     2821 atgttcttta cagcggaata atcaaaataa aacttcagaa ccagttgcgg ataagaaaat
     2881 gcctgtgtaa caggtttacc tcttgccgga gacgtttctt tgatctttat agtaactgta
     2941 gagtctttga aagtaatatc ctcaagattc agcgaataac cccccgtact cttttcgccg
     3001 gccgatacca gaacatagaa taaattcttt tcttgtttca atatcttgaa acctctggat
     3061 ttttctttaa ttatacctat ttctgcataa acctgatccg gttgcatagc ctcgtactta
     3121 aaaactggag aggcgggttg ctgagttttt gtatcagtta aataaatcga agtaggaccg
     3181 tccgacatac caattatgtt ttcacccatg gtttttatgt ctgaatgaag aatcagtatc
     3241 tcattcagtt taatcagcgc atatctcgaa gaaacatttg aagaagcatt taaagaaaga
     3301 ttatacaaag cttctactct ttcatagaaa gtttttaaag atcccttttt actgaaatct
     3361 gcattcctgt aagagtcaat ggaactttta agctctgaaa ggaaaaaatt gtcggcaagc
     3421 tcatagttaa gacattgaaa ataatactct ccaatctttt cataaaaaga gttttgaatg
     3481 aaaaaatccg atgcagcaga accttgccaa gtatcagaag agaacatcaa cgtaaaaacc
     3541 aataaaaaaa cggcaacaat caaaaacatt cttttcatgc ttattcctcc taaaaaaaat
     3601 acctgtctca tataaaaaga gatcaggtgt tttgatataa acaaaaaaat ccccgctctc
     3661 cccagaacag aataataaat aaaataataa atatccgtat aaattatata atacttattt
     3721 ctcttttgca acaaaaaaat attgtttgta acaaaaagta aaactcaaat attctacaat
     3781 ataattaaac gataaagaat cagtttgttt ttttaaaaaa atgatgatag aatgatttaa
     3841 agagtacgaa aaggggggat atttctgaac gt
//
LOCUS       DAOSHR010000185         3858 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_21659_length_3858_cov_5.121220, whole genome shotgun sequence.
ACCESSION   DAOSHR010000185 DAOSHR010000000
VERSION     DAOSHR010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 3858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3858
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21659_length_3858_cov_5.121220"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="PLU28_09060"
     CDS             <1..358
                     /locus_tag="PLU28_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35245.1"
                     /translation="EKRLHEKLSVGIGGHINPSDDAIQPTMTFFNGLNREINEEMHIK
                     NIQRLDYKGVIYDTENSVSRVHIGILFLAQAESVEIKETENFSGRWVSESELRNMDIS
                     KMETWSQIALKALDKI"
     gene            361..1140
                     /locus_tag="PLU28_09065"
     CDS             361..1140
                     /locus_tag="PLU28_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HOG35246.1"
                     /translation="MQIKAYAKVNLFLDVLDKRADGMHNILSLMHTINLYDTIYLNFS
                     DREHFETDVKFDCLWEKNSIKKAIDTFKRLTGYTDFNLSIRLVKRIPFAGGLGGSSAD
                     AAAVLRFLGNTYKIPEKDLLMISSQTGSDVPFMLNGGTAIVSGKGDIVESLETLQLNI
                     RIHPQNFQLQTSEMYKFLDETQRKQRSSDEPLKLYELLKTGNNIAARDLAFNAFEQKA
                     FELFPLLKKLKSTYSAMPDCIFCLMSGSGSSLFSVLKKGAY"
     gene            1140..2609
                     /locus_tag="PLU28_09070"
     CDS             1140..2609
                     /locus_tag="PLU28_09070"
                     /inference="COORDINATES: protein motif:HMM:NF003715.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HOG35247.1"
                     /translation="MDNLSVVLLIFSLMLILSIFSSKISSKIGLPLILLFLVIGMLWG
                     SDGPGGIYFDDPYLAQYISTVALIFILFSGGLDTEYRAIRPVLKEGILLSTLGVAITA
                     LITGIFASIIFKLPLKTGLLIGSIVSSTDAAAVFSVLRSKKISLKGNLKPLLELESGS
                     NDPMAILLTMAFTMINTGGNMTGWGFFLFFTQQILLGLICGFILGKLSLRVINRLTLD
                     YEGLYLVLIIAFTILIYSVTSVLGGNGYLAIYLSGVIFGNSNFIHKRSVLKFYEGLGW
                     LMQIVLFLTLGLLVFPSRMPPFAFNGILLSLVLIFFSRPIAVFATLLFSKLSTKEKTL
                     ISWVGLRGSVPIVLGIIPLVSNVNNADTIFNTVFFVVLTSFIIQGTTIPFMAKKLNLQ
                     NSFKQDRKQEMSFEPKNHTENTIFEMIVPEKSFAHGKEILNLGIPDKLLIILITRGKE
                     YIVPYGRTIIQSGDIITALGEKKDIDFLQGLIESKILDS"
     regulatory      2639..2740
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            2814..>3858
                     /locus_tag="PLU28_09075"
     CDS             2814..>3858
                     /locus_tag="PLU28_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HOG35248.1"
                     /translation="MKDIFGVKAAGSSVKTELYGGIATFLTMAYIIFVNPSVVVNAIP
                     GTASDPVLYNQYFGAIMVATIIASAIGTLLMGLFANYPFALAPGMGLNAYFTYTVCLK
                     LGIDWKVALGAIFMEGLIFILLTVLGVRTKITNAMPKTIKIATGAGIGLFIAYIGLKG
                     AGIVVSDSATFVTLGNMTSPQALTAVFGLIIIAVLFALKVPGSVMIGILAATIFGAFM
                     GITKIDGIVGPIPDISPTFMKLQFNWESIISPTFWMVVLTFFFVDFFDTAGTLTGLGH
                     SAGYTDKEGNLPRSERAYLADSFGTFIGAIFGTSTCTTYIESGAGIAQGARTGLASVF
                     TSAFLLLMVFFSPL"
BASE COUNT         1137 a          728 c          727 g         1266 t
ORIGIN      
        1 tgaaaaacgg ctccatgaaa aactttctgt ggggatagga ggacatataa atccttccga
       61 tgatgctatt cagccaacaa tgaccttctt taacggtctt aacagagaaa taaatgagga
      121 aatgcatatt aaaaatattc aacgtcttga ttataaaggt gtgatttacg atactgaaaa
      181 ctctgtctct agggtacata ttggtattct ttttttagct caggcagagt cggttgaaat
      241 aaaagaaaca gaaaattttt ccggcagatg ggtttcagaa agcgaattga gaaatatgga
      301 tataagcaag atggaaacat ggtctcaaat tgctttaaaa gctttagata aaatataaat
      361 atgcagataa aagcgtatgc aaaagtaaat ctttttcttg atgttcttga taaaagagct
      421 gacggcatgc acaacatttt atcccttatg cacactatca atctttacga caccatttat
      481 ctgaattttt cagatagaga acattttgaa acagatgtga agtttgattg cttatgggaa
      541 aaaaattcaa ttaaaaaagc tatagacact ttcaaacggc ttaccggtta taccgatttt
      601 aatctttcga taagattggt caagaggata ccatttgcag gaggattagg cggttcaagt
      661 gcagatgccg ctgcagtatt gagattttta ggaaatactt acaagatacc ggaaaaagat
      721 ctactaatga tttcatctca gacaggaagc gatgttcctt tcatgttaaa tggtggaacg
      781 gctatagtaa gtggaaaagg cgatattgtc gaatctcttg aaacacttca actgaatatc
      841 agaattcatc cgcagaattt tcagctccag acttcagaaa tgtacaagtt tcttgatgaa
      901 acccaaagaa aacagagatc atctgatgag ccactcaaac tttatgaact tcttaaaacc
      961 ggcaacaata ttgctgcacg agatttggca ttcaatgctt ttgaacaaaa ggcttttgaa
     1021 ctttttcctc tgctgaaaaa gcttaaatct acttattctg ccatgccaga ctgtatattc
     1081 tgtcttatga gcggttcagg aagctcattg ttttcggtac ttaagaaagg agcctactga
     1141 tggataacct ctctgttgtt ttgcttattt tctcacttat gctgatatta agtatatttt
     1201 caagcaagat ctcttcaaaa attggtcttc cgcttatact tttatttctg gttataggaa
     1261 tgctctgggg ctctgacggt cccggcggca tatactttga tgatccatac cttgctcaat
     1321 acatatctac ggtagcgctt atctttatac ttttttccgg agggcttgat actgaataca
     1381 gagctatacg tcccgttctt aaagaaggaa tccttctgtc aaccttaggt gttgcaataa
     1441 ctgccttaat aaccggaatc tttgccagta ttatattcaa gcttccgttg aagactggtc
     1501 tgctcatagg ctctatagtc tcttcaacag atgcggcagc tgtattttct gttcttagat
     1561 caaaaaaaat aagtttaaag ggaaacctga aacctcttct tgaattagaa tctggaagta
     1621 acgacccgat ggccatcctt ctgacaatgg cttttactat gataaacact ggcggaaaca
     1681 tgactggctg gggctttttc ctttttttta cccaacagat tctcttagga cttatatgcg
     1741 gctttattct tggcaagctc tccctaagag ttataaatcg tctcacgctt gattacgaag
     1801 gcttgtatct tgttctcata atagctttta caatactgat atactcggtt acatcagttc
     1861 tgggaggaaa cggatacctt gcaatatacc tttccggagt catctttgga aattcaaatt
     1921 ttattcataa acgcagtgtt ctaaagtttt acgaagggtt gggatggctt atgcagatcg
     1981 tgcttttcct tactctggga cttttggtat tcccatcccg tatgcctcct tttgctttca
     2041 acggcatact actctctttg gtacttatat ttttttcaag gcccattgca gtttttgcta
     2101 cacttttatt ttctaaactt tccaccaagg aaaagactct tatttcctgg gtaggtttaa
     2161 gaggctccgt tccgattgtt ctgggaataa ttcctctagt tagtaatgta aataacgcag
     2221 atacaatttt caacactgta ttttttgtcg tcttaacatc atttataatt caaggaacca
     2281 ccattccttt catggcaaaa aaactcaatc ttcaaaattc tttcaaacag gacagaaaac
     2341 aggaaatgtc ttttgagcct aagaatcata ctgaaaatac tatttttgaa atgattgttc
     2401 ctgaaaaatc ttttgcccac ggaaaagaaa ttctgaacct aggcattcca gataaactat
     2461 tgataatcct cataacaagg ggtaaagaat acattgttcc ttacggaaga actattattc
     2521 aatccggcga tataataact gctcttggag aaaaaaagga tattgatttt ctgcaaggac
     2581 ttattgaatc gaaaattctt gacagctgat attatttctg ataaaataat aaagtaaaaa
     2641 tttaatacgt tttttctcct atataatcct gataatatgg ctcaggagta tctaccagat
     2701 aaccgttaat tatctgacta taggaagtga agtttataaa atctgcttct cttcctttca
     2761 ttaggaagag aagtttctat ttttatctca tttctctaag gaggctgacc aaagtgaaag
     2821 atattttcgg tgtaaaagct gcgggaagtt ctgttaaaac tgaactttac gggggtattg
     2881 ccacatttct gacaatggca tacataatat ttgtaaaccc atctgtcgtt gtcaacgcta
     2941 tacccggtac agcaagcgat cctgtacttt ataaccagta tttcggggct atcatggttg
     3001 caacaataat agcttctgca ataggcactc ttttaatggg actatttgca aactatcctt
     3061 ttgcgcttgc tcccggaatg ggcttaaatg cttactttac ctatacagta tgccttaagc
     3121 tcggaattga ctggaaagtt gcactaggtg caatttttat ggaaggtctc atctttatct
     3181 tgcttacagt gctaggagta aggaccaaaa ttaccaatgc gatgcccaaa acaataaaaa
     3241 ttgcaacagg agcaggcatc ggccttttta tagcctatat tgggcttaaa ggagcaggca
     3301 tagttgtaag tgattcggca acatttgtaa ctttggggaa tatgacttct ccgcaagcac
     3361 ttacagctgt cttcggcctc ataataattg ccgtactttt tgctctcaag gttccaggct
     3421 ctgttatgat aggaatactt gcagcgacca tctttggtgc ttttatgggc ataaccaaga
     3481 ttgacggtat tgttggccct atacctgata tatctcctac ttttatgaag cttcagttta
     3541 attgggagtc tattatcagt cccacatttt ggatggtggt tcttacattc ttcttcgttg
     3601 attttttcga tacggctggt actttgaccg gtttaggaca ttctgcaggc tacacagaca
     3661 aagaaggtaa ccttccaaga tcggaaagag catatcttgc tgattccttt ggaacattca
     3721 tcggagcaat ttttggtact tccacatgta caacttacat tgaaagcggc gcaggaatcg
     3781 cacagggtgc cagaactggt ttggcttcag tatttacatc tgctttccta cttctgatgg
     3841 tgtttttttc acctttgg
//
LOCUS       DAOSHR010000186         3840 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_21810_length_3840_cov_4.305945, whole genome shotgun sequence.
ACCESSION   DAOSHR010000186 DAOSHR010000000
VERSION     DAOSHR010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 3840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3840
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21810_length_3840_cov_4.305945"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="PLU28_09080"
     CDS             <1..473
                     /locus_tag="PLU28_09080"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG35249.1"
                     /translation="SPDELMNFFGELNHISKEQLGSTKEQLVHYLGMGDFVSKPVEKL
                     STGMKQKTSIAVSLIHNPQVIIFDEPTNGLDIISSKVVTDFLKDFKKQGKTVIISTHI
                     MPEAEKLCDTIGLIFEGHLIECGTKEELFKKYSQDSIEDIFFALAEERGLIKNV"
     gene            466..1629
                     /locus_tag="PLU28_09085"
     CDS             466..1629
                     /locus_tag="PLU28_09085"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG35250.1"
                     /translation="MFKDSMIIFKKEMKNIFKDKRSFFSIIILPFILMPIIFGTIGFV
                     SNSQEKGAQETTYILQIKNAPDEFKNILSQFIKFEETQSISEEGNFAALEFPQTYNTD
                     SLEKINVKVLFRSTSQKSSFALGQIKAALNAYQNLILQKNLEKHDLSLDDLKPINISE
                     VDIAPKESQGTQFLSMMVPYMLLIYIFAGAMGIGMDTTSGEKERGGLAALLVNQVSRT
                     SIAIGKILYVIVTGLLNSVSTFGGLIVGISLIGNFSGGSSELYIPAFSAFSILGLLFT
                     ILSMSAVAASMIILMGSFARNVKEASGYIMPVYIIAIVVGVATMNMDFTSSIGISVIP
                     LIGSVFSLKGLLSNQISLLQVLLSIFSNFAVSFLFAYVTAKLFNSEKIMNTVG"
     gene            1639..2391
                     /locus_tag="PLU28_09090"
     CDS             1639..2391
                     /locus_tag="PLU28_09090"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOG35251.1"
                     /translation="MSILKTFTIGKTGKRIVGITERPDNPGKYPVILMFHGFSGDHIV
                     SGFKFPRVSRLLVEQGFATVRFDFRGSGDSEGNFEDMSILTELSDALEVFGYIKKKDF
                     YNHKLGIIGYSMGGAVASLFAPKVQDTNAVCLWSPAIFNKEVFQSGLMIKKLSEQSFI
                     DIGGLKISTVFAQDCEKTDAFENLLKYRKKLRIIHGSKDESVDFTKVKNFCIQNSINF
                     HQIENADHKYLSVGYMNELFNQTVDFFCASMK"
     gene            2404..3084
                     /locus_tag="PLU28_09095"
     CDS             2404..3084
                     /locus_tag="PLU28_09095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HOG35252.1"
                     /translation="MDYTVDFKGSYSMLRVRLNGGESIKSEPGSMLSMSGNIEIQTDT
                     GGGFLKAVKRSFLGGESFFMNTFYASSEAEVCLAPELPGDIEVVDINNELFVQSTSFL
                     ACDTGIDMDTRFGGMKSFFSGEGLFMLHLRGSGRVAVSSFGAIFKKYIAPGEVLTVDT
                     GHIVAFDGSVSYSIDKFGSWSSTILGGEGLVCRFTGPGNVYIQTRNVPAFINWIKGYI
                     PTRETGSR"
     gene            3207..>3840
                     /locus_tag="PLU28_09100"
     CDS             3207..>3840
                     /locus_tag="PLU28_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35253.1"
                     /translation="MEKRVFLICALFVCFMGILPANPFKLQLEYEKGFVGPVFHQIQF
                     GKTENGNTKFDYVKDGGQDILFPAEKFTLRFEPAQSHLITFVYQPLELQTKAYLTSDA
                     TINTVKFEKNSGLSLKYSFPFYRVSYMYKFINEKSFDIGAGLSLQLRNASISFESLDG
                     KKIIINQNLGPVPILKFAANYRFENGIFLGTEIDGFYASSAFINGSSFDFE"
BASE COUNT         1200 a          732 c          774 g         1134 t
ORIGIN      
        1 tttcacctga tgaactgatg aattttttcg gtgaactgaa tcatatttca aaagaacagc
       61 tcggttcaac taaggaacag cttgttcatt accttggaat gggagatttt gttagtaaac
      121 ccgtagaaaa actttccacc ggtatgaaac agaaaacatc tattgcggta agccttatac
      181 ataatccgca ggtaattata ttcgatgagc ctacgaacgg tcttgacata atttcatcca
      241 aagtggtcac agatttttta aaggacttca aaaaacaggg gaaaacagtc ataatttcaa
      301 cccacataat gcctgaagcc gaaaaacttt gcgataccat aggtctcatc tttgaaggtc
      361 atcttattga gtgcggaacc aaagaggagc tttttaaaaa atactcccaa gacagcattg
      421 aagacatttt ctttgctctt gcagaagaaa ggggactgat taaaaatgtt taaagattcc
      481 atgataattt tcaaaaaaga gatgaagaat atttttaaag ataaacgttc ttttttctcc
      541 atcatcattc ttccgtttat tcttatgcct ataatcttcg gcactatagg atttgtatca
      601 aactcacagg agaaaggtgc acaggaaaca acctatatcc ttcagataaa aaatgctccg
      661 gatgagttca aaaacattct ttcacagttc atcaaatttg aagaaaccca atccataagc
      721 gaagagggaa actttgcggc acttgagttt ccgcaaacgt acaacacaga ctcccttgaa
      781 aagataaacg taaaagtgct tttcagatcc acatcgcaaa aatcatcttt tgcactcgga
      841 cagattaaag ctgccctaaa cgcatatcaa aatctgatac tgcagaaaaa tcttgaaaag
      901 catgacctat cactggatga ccttaagcct attaacatat ctgaagttga cattgctcca
      961 aaagaatccc agggaaccca gttcctgtca atgatggttc cttatatgct ccttatctat
     1021 atttttgccg gagcaatggg tataggcatg gatacaactt caggtgaaaa ggaacgcggc
     1081 ggtcttgctg cccttttagt caaccaggta tccagaacat ctatcgctat aggaaaaatt
     1141 ctttatgtta tcgtaaccgg actgcttaac agcgtttcca cttttggagg gctaattgtg
     1201 ggaatatctc ttataggaaa tttttctgga ggaagcagcg aactttatat accagccttt
     1261 tcggcatttt ccatactcgg actcttgttc accatacttt caatgagtgc agtggcagct
     1321 tcaatgataa ttcttatggg aagctttgca agaaacgtta aagaagcctc cggctatata
     1381 atgcctgtgt atattatcgc aattgttgtc ggcgtagcta ctatgaatat ggattttaca
     1441 agttcgatag gcatctctgt cattcctctc ataggatcag tgttttccct caaaggactt
     1501 ctgtcaaatc agatatcact tttgcaggta cttttatcca tattcagtaa ctttgccgta
     1561 tcttttcttt ttgcatacgt aacggccaag ctgttcaact ctgaaaaaat catgaacact
     1621 gtagggtgag gcggtggaat gtcaatatta aagaccttca caattggaaa aaccggaaaa
     1681 agaatcgttg gaataacaga acgtcctgac aatcccggaa agtacccggt aattttaatg
     1741 ttccatggct tttccggcga ccatatcgta tcaggcttca aatttccaag agtatcaaga
     1801 cttcttgttg agcaaggctt tgctaccgta aggtttgatt tcaggggatc tggcgatagt
     1861 gaaggaaatt ttgaagatat gagcatactt acagagcttt cagatgctct tgaggtattt
     1921 ggatatatca agaaaaaaga tttttacaac cacaagcttg gaattatcgg atacagtatg
     1981 ggcggagcag tcgcgtctct attcgctcca aaggttcaag ataccaatgc agtatgctta
     2041 tggtctcccg ccatattcaa caaggaagtt tttcaatctg gtctgatgat aaaaaaactc
     2101 tcggaacaat cttttataga tataggcgga ttaaaaatat caacagtgtt cgcacaggac
     2161 tgcgaaaaga ccgatgcatt tgaaaacctt ctaaaatacc ggaaaaaact gagaataata
     2221 cacggttcaa aagatgaaag cgtagatttt acaaaagtaa agaacttctg catacagaac
     2281 agtatcaact ttcatcagat tgaaaatgca gatcacaaat atctaagcgt tggctacatg
     2341 aatgaacttt tcaaccagac agttgatttt ttctgcgcaa gcatgaaata aaaggaggaa
     2401 aagatggact atacggtaga tttcaaaggt tcctacagta tgctaagagt aagactgaac
     2461 ggcggtgaat ccataaaaag cgaaccggga tcaatgctca gcatgtcagg gaatatagag
     2521 attcagactg atactggagg tggattcctt aaagctgtca aaagaagctt ccttggcggc
     2581 gaaagttttt tcatgaatac attttatgct tcgtcagagg cggaagtatg tcttgctcct
     2641 gagcttccgg gagatatcga ggtagtcgat atcaataatg agctttttgt acagtcaact
     2701 tcatttcttg cctgtgatac cggcatagat atggataccc gtttcggcgg catgaaatcc
     2761 tttttttctg gtgaagggtt gtttatgctc catctgcgcg gcagcggcag agttgctgta
     2821 tcttctttcg gcgctatatt taaaaaatat attgctcccg gtgaagtgct aaccgtcgat
     2881 accggtcata tagttgcatt tgacggctct gtcagctact ccatcgataa gttcgggagc
     2941 tggtcctcaa ctatacttgg cggtgaaggt cttgtgtgca gatttaccgg accagggaac
     3001 gtctatattc agaccagaaa cgttccagca ttcataaact ggataaaggg atatattcct
     3061 acacgtgaaa ccggaagcag ataatcttta ataaaatatc cgtcctgata cagatgaagg
     3121 acgggtattt tattttttat gtttattaat gtataatatt aatatatgta actcaacaat
     3181 aatctaaaga caaaggggtg atccgcatgg aaaaaagagt tttcctgata tgcgctttat
     3241 ttgtgtgctt tatgggaata ctgccggcaa atccttttaa gcttcagctg gaatatgaaa
     3301 agggttttgt agggccagta tttcatcaga ttcagtttgg taaaacagaa aatgggaata
     3361 ccaagtttga ttatgttaaa gacggcggtc aggatatact tttccctgcc gagaagttca
     3421 cactgcgctt tgaaccggct cagagccatc tcattacctt tgtgtaccag ccgctggaac
     3481 tgcagactaa agcctatctc acatcagatg ccacaattaa cactgtgaag tttgaaaaaa
     3541 actccggtct gtctttaaaa tactcttttc cgttttacag agtcagctat atgtataaat
     3601 ttataaatga aaagagtttt gatattggag ccggtctttc tctacagtta aggaatgcca
     3661 gtatatcctt tgaaagcctg gacgggaaaa aaattataat aaaccaaaat cttggacctg
     3721 ttcctattct taagtttgct gctaactacc gctttgaaaa cggtatcttt ttgggaacag
     3781 agatcgatgg gttttatgct tcatcagcat tcataaacgg aagttcattt gattttgaag
//
LOCUS       DAOSHR010000187         3839 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_21817_length_3839_cov_5.219345, whole genome shotgun sequence.
ACCESSION   DAOSHR010000187 DAOSHR010000000
VERSION     DAOSHR010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 3839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3839
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21817_length_3839_cov_5.219345"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..564
                     /locus_tag="PLU28_09105"
     CDS             <1..564
                     /locus_tag="PLU28_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35254.1"
                     /translation="LFFFFLCRSDNFFSNNHWFSLVVTAVMLFLIIISSIYNSVKKRD
                     SIKRYLYSILVYIPLFMMNFFQDNPIVINILAFIPLPMAVFYFVFLYRYPFYSQYWVE
                     GSIDEILSQVEKGCGYSPKPITVFKSTGKRFITGCRGLKIMFREEKTIVRMSRKYHKA
                     CGSPNLKEFSDRITEKIIERYRGEADE"
     gene            557..754
                     /locus_tag="PLU28_09110"
     CDS             557..754
                     /locus_tag="PLU28_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35255.1"
                     /translation="MNDWFLIIVCVAALGLLIFRQAIKYKQYKEEDRVKDFFKELLFM
                     LLRMAVILAVLFLISFLTGTH"
     gene            776..1525
                     /locus_tag="PLU28_09115"
     CDS             776..1525
                     /locus_tag="PLU28_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG35256.1"
                     /translation="MIEVKGLVKKFGEFTAVNKVDLSIKGGEIYGFLGPNGAGKTTTI
                     RMLTGTLNPTEGDIRILDKDIRKDDIQIKSEIGVVPDEPKMYENLRGFEYVEFIMGIY
                     KVDKTEAKKRLDEICEAFDIDFLNDYIGDYSHGMKQKLMVATVLMRKPKVIFLDEPTV
                     GLDAKSAKILKMLIQKYRDEGSTIFLTTHILEIAEKMCDRIGIIKDGSIIAQGTMEEL
                     RSKSTQHNKSLEDIFLELTGDEEIDNIVKEL"
     gene            1536..3134
                     /locus_tag="PLU28_09120"
     CDS             1536..3134
                     /locus_tag="PLU28_09120"
                     /inference="COORDINATES: protein motif:HMM:NF021374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35257.1"
                     /translation="MSDLSLLLKYSFKNKVRPAKNKKGVSKASKPITTVIQYLLPAVI
                     FGMTIAPVMFITMKDLNIPMQSLGIDSSYSFLDIIFSLNFLGMGIMFFMNYSPSIIMN
                     LYDSEMTQAFLTMPIKKSTIFWAGSIDSLLMTGLPLGMLLPIAVVYAIIMKANIFLSI
                     LAIVGFVIVLLGISMLGGILMSFFMSRTSAKRFTMFAYFGSIILYVFAANYFNPSNFN
                     SGNVEVIAQTFKNALGFILNPVFPHTWMINSTKGNLWSLIILFGAGILLFCFTYFLSQ
                     KLDFSISGKKAKKSKKITFKTGRFPLIKKDLKLSFRDSQTLFLLIYPAVLPVIFFFAG
                     NTKGSSLGVMSSLMFSAIAAFYAAYLAIMMLAEETKIWPVPKLYPIKTRTLVSNKVNI
                     PFAIFMIEYILIVILLTVISGFSFVNIISILPMGVILYYSSLLGARLFLKNPKREVKQ
                     KNFLSGKEVFELEGATMGVAFGVFFLLNFFSTVKNRPFWIFVGREWLIYLVFIGVPIL
                     LIGLTVLGIFKELKKIKVLIENWE"
     gene            complement(3162..>3839)
                     /locus_tag="PLU28_09125"
     CDS             complement(3162..>3839)
                     /locus_tag="PLU28_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35258.1"
                     /translation="GLVALFGAGEFIFKSSKQGPIQRIENDFPSNSMIKVYTLNSDTD
                     WTKFNLSCGNGEQAFRMKLSMDKDIFRMKTSYEEGTLNIIDKLKVSNVKVPERSNLEL
                     FMDKNLEYKIDSTFNVSDVLLDMRELNIKNAKLNTTATKISIVPTDVADSVIDMNIEI
                     SALTIKVPKNVSLVLNHVGDLTFKTLEGLVKREDGSYVSSNYSQTSATCYLNISSEMS
                     RLNIVLI"
BASE COUNT         1209 a          675 c          719 g         1236 t
ORIGIN      
        1 ttgttctttt tctttttgtg ccgcagcgat aactttttca gcaataatca ttggttttct
       61 ttggtagtta cagcggtaat gctttttctt attataataa gtagcatata caactctgtt
      121 aaaaagagag acagtataaa acgatatctc tattcaatcc ttgtatatat tcccttattt
      181 atgatgaatt ttttccagga taatcccata gtaattaaca ttctggcttt tatcccgctt
      241 cctatggctg tattttattt tgtttttctt tacagatatc ctttttacag ccagtactgg
      301 gttgaaggtt caattgatga gattcttagt caggttgaga aaggatgtgg atactcacca
      361 aagcctatca cagtttttaa gagcaccgga aaaagattta taacaggctg cagaggtctg
      421 aagatcatgt tccgagagga aaaaacgata gtacgtatgt cccggaagta tcataaagcc
      481 tgcggaagtc caaaccttaa agaattttca gaccgaataa ccgaaaaaat aatcgaaagg
      541 tacagaggtg aagcagatga atgactggtt tcttattatt gtatgtgttg cggcattagg
      601 actgttgata ttcagacagg ccataaagta caaacagtat aaagaggaag acagggtaaa
      661 ggattttttc aaagaacttt tgttcatgct cctgcgtatg gcagttattt tagcagtact
      721 gtttcttata tcgtttctta ccggtacaca ttaaaattat agaaggagga aaaagatgat
      781 agaagtaaaa ggacttgtta aaaaatttgg tgagttcaca gcagtaaaca aagtagatct
      841 ctcaataaaa ggtggggaaa tatacggttt tctcggtcca aacggtgcgg gtaaaaccac
      901 tacaatcaga atgcttaccg gaacccttaa tcctaccgaa ggagatatta gaatactgga
      961 taaggacatt cggaaagacg atattcagat taagagtgag atcggtgtag ttcctgatga
     1021 acctaagatg tatgaaaacc tcagaggctt tgaatatgtc gagtttataa tgggtattta
     1081 taaagtagat aaaactgagg caaaaaagag attggacgag atctgcgagg catttgatat
     1141 cgatttttta aatgactata taggtgatta ttcacatggt atgaaacaaa agctcatggt
     1201 agcaacagtg cttatgagaa aacccaaagt catctttctt gatgaaccta ccgttggtct
     1261 tgatgccaaa tctgcaaaaa ttctcaagat gcttattcaa aagtacagag atgaaggatc
     1321 aaccattttc cttactactc atatcctgga aatagctgaa aagatgtgtg acagaatagg
     1381 aataataaaa gacggttcaa taattgcaca gggaaccatg gaagaactac gttcaaaatc
     1441 aactcagcac aataagtcgc ttgaagatat cttccttgaa cttaccggcg acgaagagat
     1501 agacaatatc gtaaaggaac tttaggagga agatcatgtc agacctttca ttattactca
     1561 agtattcatt taaaaataaa gtacggccgg caaaaaacaa aaaaggcgta tccaaggcat
     1621 ctaaacctat tacaaccgta atccagtacc ttcttccggc ggtcatattc ggtatgacaa
     1681 ttgctccggt aatgtttata accatgaagg atctcaatat acccatgcag agccttggta
     1741 tcgacagcag ttattcattt cttgatatta ttttttctct taactttctg ggtatgggaa
     1801 taatgttttt catgaactac tctccaagca taataatgaa cctttacgac agtgaaatga
     1861 ctcaggcatt tctcactatg cccataaaaa aatcaactat attctgggcc ggctccatag
     1921 atagtcttct catgaccggt ctgcctttag ggatgcttct tcccatagca gttgtttatg
     1981 ctataataat gaaagccaat atatttctat cgattttggc tatagtaggc tttgttattg
     2041 ttcttcttgg aatttcaatg cttggaggca tactgatgtc attttttatg agtagaacat
     2101 ctgccaagcg gttcacaatg tttgcatact ttggaagtat aatactgtat gtttttgcag
     2161 ccaattactt taacccctca aacttcaact caggaaacgt cgaagtaatt gcacagactt
     2221 ttaagaacgc actgggtttt atcctaaatc ctgtctttcc gcatacatgg atgattaaca
     2281 gtacaaaagg taatctatgg tcactgataa tacttttcgg agcaggtata cttttgtttt
     2341 gttttactta ttttttatct cagaagctgg acttttcaat ctcaggaaaa aaagccaaaa
     2401 aaagtaaaaa gattactttt aaaaccggaa ggttccctct gataaaaaaa gatctcaagc
     2461 taagcttcag agacagtcag acattatttc ttcttatcta tccggccgtt ttacctgtta
     2521 tctttttctt tgcaggcaat acaaaaggtt cttctctcgg agtaatgtct tcgctcatgt
     2581 tttcggctat agcagctttt tatgctgcat accttgccat aatgatgctt gcggaagaaa
     2641 ccaagatatg gcctgttcca aagctttatc ctataaaaac cagaactctt gtaagcaaca
     2701 aggttaatat tccctttgct atctttatga tagaatatat tcttatcgta atactgctaa
     2761 cagtaatttc gggattttcg tttgtaaaca taataagcat cctgcctatg ggagttatac
     2821 tctactactc gtccttactt ggagcaaggc tttttcttaa aaatcctaaa agagaggtaa
     2881 aacagaagaa cttcctttcc ggaaaagaag tatttgaact ggaaggagca actatgggag
     2941 tagcttttgg ggtatttttc cttcttaatt tcttttcaac agtaaaaaat aggccatttt
     3001 ggattttcgt cggacgggag tggcttatct atcttgtatt tataggtgta ccaattcttc
     3061 tgataggact gacagtactg gggatattta aagaactgaa gaaaattaaa gtacttatag
     3121 aaaactggga ataaaaaaca gaatctttat gatcctgttt tttatataag tacaatattg
     3181 agtcttgaca tttcagaaga tatattcaga taacaagtcg cactggtctg tgagtagtta
     3241 gaagaaacat atgaaccgtc ctcccgtttt acaagccctt caagggtttt aaaggtaaga
     3301 tcccccacat ggtttagaac caatgaaaca ttcttgggaa cctttatcgt aagggctgat
     3361 atctctatat tcatgtctat cactgaatct gcaacatctg tcggtacaat tgaaatcttg
     3421 gttgctgtgg tattgagctt ggcattttta atattaagtt ccctcatatc caaaagcaca
     3481 tcagagacat taaatgttga atcgatttta tactcaaggt ttttatccat gaagagttca
     3541 agattggatc tctctggaac tttaacgttt gaaactttga gcttgtctat tatgttcaaa
     3601 gtaccctctt catatgatgt tttcattctg aagatatctt tatccattga aagtttcatc
     3661 ctgaaagcct gttctccgtt cccgcatgaa agattaaact tggtccagtc agtatcagag
     3721 ttaagtgtat atacctttat catcgagttt gaaggaaaat cgttttctat tctctgtatg
     3781 ggaccttgct ttgatgattt gaaaataaac tctcctgctc caaaaagtgc gaccagtcc
//
LOCUS       DAOSHR010000188         3823 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_21953_length_3823_cov_4.569268, whole genome shotgun sequence.
ACCESSION   DAOSHR010000188 DAOSHR010000000
VERSION     DAOSHR010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 3823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3823
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21953_length_3823_cov_4.569268"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..984
                     /locus_tag="PLU28_09130"
     CDS             <1..984
                     /locus_tag="PLU28_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35259.1"
                     /translation="KDLSLFALTDAGAHASVHFWPIGLGTYAYLKGSMDLSVPNRLID
                     LIANGNKINETYTASSQIFGDLYIDAGAYGALRSDSFALGVKYGYFMPIAKSQNSVYN
                     FKFDTNSQNSTMAATLTGDFSYVSPFSYSQLKNFSAEDIQHEFRNKAGQKLDIGFVTG
                     ENRKPFLGVSLNNITLVPAKLDRKNNVTATATVSYNNPLFGGKVDISDPVIKTDETVL
                     YEPILADAPLNMSAFIRLPFILDWIPYIQYYFNDSRVDWGVNAQGNLLWILPFNVGLE
                     SINNSWKASAGMGIDIYLVQLYAQVSSRSRNFDMLMNTNNMAFRVDIGVGF"
     gene            1157..1411
                     /gene="acpP"
                     /locus_tag="PLU28_09135"
     CDS             1157..1411
                     /gene="acpP"
                     /locus_tag="PLU28_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOG35260.1"
                     /translation="MTREELFTKVKDIVVETLNVDEADVTLDASFIDDLDADSLELVD
                     LTMAIESELGITIEDEELEGIKTVEDVVQAIAEKLDIDEE"
     gene            1498..3078
                     /locus_tag="PLU28_09140"
     CDS             1498..3078
                     /locus_tag="PLU28_09140"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOG35261.1"
                     /translation="MAQKYIIVSGGVLSGIGKGVVSSSIARIMKEAGRKVSSLKIDPY
                     LNVDAGTMNPNQHGEVFVTDDGYEADLDLGHYERFSGINMKRCNNITAGQIYKHVIEK
                     EREGMFLGGTVQMVPHVTNEVKNHIKSIDTDLLIIEIGGTVGDIEAEIFLEAVRELSV
                     ETGREDFMFIHVTYVPFLKVTNEFKTKPTQQSIQTLRKIGIQPDMIVVRTESEIDRQS
                     MEKVSLFGGVAKEYVINLPDLGNIYEVPEKLHNLNIHKLISQKLRIDVSDEFSWNPPR
                     VFDDLKIALIGKYLGTDDAYKSILESIFLCGARKPELIDAELLEDKSEDEVKRMLEQF
                     KGIIIPGGFGKRGTEGKINAIKYARKNKVPILGICLGLQTMVIEYARNVAGLEGANST
                     EFDPDTKYPVIDIMEEQKKILKLGGTMRLGSQETRLFKNTKVYDIYGEERIFERHRHR
                     FEVNMERFAEIFTTADKNEPGKLMVSGISDFVEVVELKDHPFFIGVQYHPELKSKVGN
                     PGPLFAALVDTIRKMEKK"
     gene            3075..>3823
                     /gene="rsmA"
                     /locus_tag="PLU28_09145"
     CDS             3075..>3823
                     /gene="rsmA"
                     /locus_tag="PLU28_09145"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOG35262.1"
                     /translation="MNTLQWIRKYGLHLKKSFGQNFLNSEETVQKIVDTALIKQDETV
                     IEIGPGAGTMTEKLAASGANIIAYEADLSLKELLTERFCSYKNVQILFEDFLRADLAV
                     HPPKVKYVANIPYNISSPILQKIFSSGLVFEKAVLMVQKEFGERMAAVQGKEYSPLSI
                     FVQYYCTVKEEFIVPRTEFIPSPKVDSMVVSLTPYRGFSPDDKDFFGFIHVCFSQRRK
                     NIKNNLRPILDDPTDFLLRCGIDPLLRPEQIP"
BASE COUNT         1247 a          703 c          831 g         1042 t
ORIGIN      
        1 aaagaccttt cacttttcgc acttaccgat gcgggagcac atgcatctgt acatttctgg
       61 ccgatcggac tgggtactta tgcttatctg aaagggagca tggatctgag cgtaccgaac
      121 aggctcatag atctaattgc aaacggcaat aaaataaatg aaacatatac tgcatcatct
      181 cagatcttcg gcgatttata catagatgcc ggcgcatacg gtgcattaag aagcgacagc
      241 tttgcattgg gagtaaagta cggttacttc atgcctattg caaagtctca gaactctgtt
      301 tacaacttca aatttgatac caattcacag aacagcacca tggctgcaac cctgactgga
      361 gacttctcat acgtaagtcc tttttcgtac tctcagctta agaacttttc agcagaggat
      421 atccagcacg agttccgtaa caaagcaggt cagaaacttg atatcggttt tgtcaccgga
      481 gaaaacagaa agcctttcct aggagtttct ttgaataata taactcttgt tcctgctaaa
      541 cttgacagaa aaaataatgt aacagctacc gcgacagtat cttataataa tcctcttttc
      601 ggaggaaagg ttgatatttc tgatcctgtt ataaaaactg atgaaactgt actttacgaa
      661 ccgatacttg ccgatgctcc acttaacatg tctgcattca taagactgcc atttattctg
      721 gattggatac cttacattca gtattatttc aatgactcca gagttgactg gggagtaaac
      781 gcacagggta atctgttatg gatacttccg ttcaatgttg gattggaaag cattaacaac
      841 agctggaaag cttcggccgg aatgggaata gatatttatc tcgtacagct ttatgcacag
      901 gtttcaagca gaagccgaaa cttcgatatg cttatgaata ccaataatat ggcctttagg
      961 gtagatatcg gagtaggttt ctaaacaaaa aaatcttgtt catatattca gcttttaaat
     1021 ttataaaaat tttacttatc tgtaggattc atacttgaca aaccaaaaaa gaataaatat
     1081 aattaagtag aatttggatt aaaaattaag atcggttttt taacaacaaa agcagttttt
     1141 tgggaggctg aacgttatga caagagaaga actttttaca aaggttaaag atatcgttgt
     1201 ggagacacta aacgttgatg aggctgatgt cacccttgac gcctcgttca tagatgatct
     1261 tgacgctgat tcgcttgaac tggttgatct tacgatggct atagaatccg agcttggaat
     1321 aaccatcgaa gatgaggaac ttgaaggaat aaagactgtg gaagatgtag ttcaggcaat
     1381 tgcagaaaaa ctggacatcg acgaagaata accgagaatt cgggggagcc ttgctcccct
     1441 tttttatttt aaatttttcg gaccaccata aggcataata aacacgaggt gaaaagtatg
     1501 gcgcagaagt acattattgt atccggcggg gttctcagcg gaatcggaaa aggggtggta
     1561 tcttcttcga tcgcccgtat aatgaaggaa gccggaagaa aggtaagcag tttaaaaatc
     1621 gatccttatc tcaacgttga tgcaggaact atgaatccca accagcacgg tgaagttttc
     1681 gtaaccgatg acggatacga agctgatctt gacttgggtc attatgagag attttcaggc
     1741 attaacatga agcgttgcaa caacattacc gcaggacaga tatataaaca tgtaattgaa
     1801 aaagagcgtg aaggaatgtt tctcggtggc acagtccaga tggttccgca tgtcacaaac
     1861 gaagtaaaaa accatattaa aagtattgac acagatcttc ttatcataga gattggagga
     1921 actgtcggtg atatcgaagc agagattttt ctggaagcgg taagagagct ttcagtagaa
     1981 acaggcaggg aagactttat gtttatccat gtaacgtatg ttcctttcct caaggttacc
     2041 aatgagttta aaaccaaacc cactcagcag tctattcaga ctctgagaaa aataggaata
     2101 cagccggata tgatagttgt caggacagag agcgaaattg accgtcagag catggaaaaa
     2161 gtttctcttt ttggtggtgt agcaaaagaa tatgtaataa acctacctga cctaggcaac
     2221 atttatgagg taccggaaaa acttcataac ctgaatatcc ataagcttat ttcacagaaa
     2281 ctgagaatag atgtttcgga tgagttctca tggaatccgc cacgcgtatt cgatgatcta
     2341 aaaattgctc tcataggaaa gtatcttgga acagacgatg cttataaaag cattctggaa
     2401 agcatctttc tctgcggtgc caggaaacct gagctcatag atgccgaact attagaggac
     2461 aaaagtgaag acgaagtaaa aaggatgctt gagcagttca agggaatcat cattccggga
     2521 ggtttcggaa aacgcggaac cgaaggtaaa ataaatgcca tcaagtatgc aaggaaaaat
     2581 aaagttccca tcctcggaat ctgtttagga ttacagacaa tggttattga gtatgcaagg
     2641 aatgtcgcag gacttgaagg agccaattca acagagtttg atcctgatac taagtatccg
     2701 gtcatagata ttatggaaga acaaaaaaag attcttaagc taggaggaac catgagactt
     2761 ggctctcagg aaaccaggct tttcaaaaac accaaagtat acgatatata tggtgaagaa
     2821 agaatctttg aacggcacag gcacagattt gaggtgaata tggaaaggtt tgcggaaata
     2881 tttaccacag cagacaaaaa cgaacccgga aagcttatgg tgagcggtat ttcagatttt
     2941 gtagaagttg tggagcttaa agaccatccg ttttttatag gtgtacagta tcatcctgaa
     3001 cttaagtcca aggtaggaaa tccgggtcct ctctttgctg cccttgtaga caccataaga
     3061 aagatggaga aaaaatgaac actttacagt ggataagaaa gtacggactt catcttaaaa
     3121 aatcattcgg gcagaacttc ctaaattctg aggaaacagt ccaaaaaata gttgatactg
     3181 ctctgataaa gcaagatgaa acggttattg aaatcggtcc cggagccgga actatgacag
     3241 aaaaacttgc cgcaagcgga gcaaatatta ttgcatacga ggctgacctg tcattgaagg
     3301 agcttctcac tgaaagattt tgcagttata aaaatgtaca gatactcttt gaagactttc
     3361 taagggcaga tctggcagta catcctccaa aagttaagta tgtggccaat attccatata
     3421 atatatcctc acctatcctc cagaaaattt tttcatctgg gcttgttttt gaaaaagctg
     3481 tactgatggt tcaaaaagag ttcggagaac gtatggctgc agtacaagga aaagaataca
     3541 gtccgctgag tatatttgta cagtactact gcaccgtaaa agaggagttc attgttccgc
     3601 gtaccgagtt tattccgtct ccaaaggtcg attccatggt ggtttctctg actccttacc
     3661 ggggatttag cccagatgac aaggattttt tcggatttat tcatgtttgt ttttcccaga
     3721 gaaggaagaa tattaagaat aatctcagac ctatcctgga cgatcctacg gattttcttt
     3781 taagatgcgg aatagatcct cttctcagac cggagcagat tcc
//
LOCUS       DAOSHR010000189         3816 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_22016_length_3816_cov_5.637596, whole genome shotgun sequence.
ACCESSION   DAOSHR010000189 DAOSHR010000000
VERSION     DAOSHR010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 3816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3816
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22016_length_3816_cov_5.637596"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="PLU28_09150"
     CDS             complement(<1..308)
                     /locus_tag="PLU28_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HOG35263.1"
                     /translation="MPINLKGRSFLTLRDFTTEEIMYMLNLAKDLKAKKRMGVRGESL
                     KGKNIVLIFDKASTRTRTAFEVATFDEGGCVTFLTNSQMGKKESIEDTAKVLGRSYDG
                     "
     gene            complement(351..935)
                     /locus_tag="PLU28_09155"
     CDS             complement(351..935)
                     /locus_tag="PLU28_09155"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HOG35264.1"
                     /translation="MKTGKRCPWSDSSELYRIYHDTEWGVPVHDEKKHFEFILLEGVQ
                     AGLSWITILKKRENYRSAYDNFDYEKIALYDEPKVLQLMGNTGLIRNERKIRASITNA
                     QAFVRIQKEFGSFDKYIWGFVNFKPIINHFETLSEIPVKTELSDKISSDMKKRGFTFF
                     GSVICYAHMQAIGIVNDHLTCCFRHQELSEKFNF"
     gene            complement(937..2154)
                     /gene="arcA"
                     /locus_tag="PLU28_09160"
     CDS             complement(937..2154)
                     /gene="arcA"
                     /locus_tag="PLU28_09160"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HOG35265.1"
                     /translation="MNLNVYSEIGNLKKVMLHRPSKELENLAPEYLQELLFDDIPYLE
                     KAQQEHDEFASILRNEGVEVVYLLDLLQQTLSSWELKSEFLREFIDMSEITNPYISQA
                     LYDFLVSMDTRAMIEKLVCGLRTEELGLKKDIFSVQVRKSKLNHPFFLDPLVNLYFQR
                     DPVAVVGRGATINRMNTPARRRESMFMEYVLKYHPDFSRSPFYYSKDMPYSIEGGDIL
                     ILNRNTLAIGISQRTEPEALEIIAKKVFYEYGDEFDTIIGVLIPSLRAFMHLDTVFTM
                     VDYDKFVVHSKLEKNMKFFTIRKKEKDEFEIAEETAPLNVVLEKYLKIKKVTLIKCGG
                     SDDIAASREQWNDGSNSLAIRPGVVVVYDRNRITNAELKKYGITTLEMPSSELSCGRG
                     GPRCMSMPLFRQD"
     gene            2388..>3816
                     /locus_tag="PLU28_09165"
     CDS             2388..>3816
                     /locus_tag="PLU28_09165"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOG35266.1"
                     /translation="MKSIIKSVSRYFLFTRIMLLCFGIVIILVVYKYFQMKEIGNVYY
                     RIGNEFSKMLEEGYLAKGNNIITVNSSDEIYKKLESIFSSVGQINKTLSEPVSLDEIE
                     YFFNRSSFDIDELDRAIRKVKDYMYESYLYVNKGFTFIFTISVLIALVISGIRLFFEL
                     KAKKVFVSYNDYVKNMSEKVLGLIKQNAPSQNIPIRWLEEKELYDNVSSIEKELGLEK
                     RIISLPVYGTLEDLVPQLMPFLKEYIQCDRVGVAFIDSVENVVAETAASYTENLKLGA
                     GFIQNINDTSLKWIVENRSPRIINDLEYYYKNFHKSRATELILQEGYRSSLTVPIITN
                     DKVVGFLFVNSVNKDAYTNECSQIMLRVMNILKGTTFYSYITQQIVSTSAQAFVSLVE
                     KKDNETGFHISRVSQYSYTIVNQMSRVFPMITPKFRREILWFASLHDIGKIGIPDNIL
                     LKPGKLTPEEFDIMKQHVLFGENILK"
BASE COUNT         1150 a          705 c          727 g         1234 t
ORIGIN      
        1 attccatcgt aagatcttcc gagaaccttg gcggtatctt ctattgattc ttttttgccc
       61 atctggctgt tggtaaggaa ggtaacgcat cctccctcgt caaaagtggc aacttcgaag
      121 gctgtcctgg ttcttgtgga agctttgtca aatataagga cgatattttt tcccttcagt
      181 gactctcccc ttacacccat tctttttttt gccttaagat cctttgcaag gttaagcatg
      241 tacatgatct cttccgtggt gaaatccctg agagtcaaaa aacttcttcc tttgaggtta
      301 ataggcattt tcttctcctc ctgtatagta ttttaaaatg cgcttatccg ttaaaaatta
      361 aatttttcag agagctcctg atgtctgaag cagcaggtta aatgatcatt tactattcct
      421 attgcctgca tgtgggcata gcatattaca gaaccgaaga aagtaaaacc gcgttttttc
      481 atatcactac tgattttgtc tgaaagctcg gtttttacag ggatttctga aagcgtttca
      541 aaatggttta tgatcggttt gaaattcaca aaaccccata tatacttgtc aaaagaacca
      601 aactctttct gtattctcac aaaagcctgt gcattggtta tgcttgcccg tatctttctt
      661 tcgtttctta tcaatcctgt gtttcccatg agctgcagta ccttcggttc atcataaagg
      721 gcaatttttt cataatcaaa gttatcgtat gcactgcggt agttttccct ttttttcaga
      781 atggttatcc agctcagtcc ggcttgtact ccttcaagaa gtataaattc aaaatgtttt
      841 ttttcgtcgt gcaccggaac tccccactcg gtatcatgat agattctgta cagttcggac
      901 gaatcggacc acggacatct tttaccggtt ttcatatcag tcctgcctga aaagaggcat
      961 actcatgcat cttggaccgc ctctgccgca cgaaagttcg cttgaaggca tttcaagagt
     1021 tgttattccg tattttttta gttcggcatt ggttattctg ttacggtcat agaccacaac
     1081 tactcccggt cttatggcaa gagagtttga accgtcattc cattgttccc ttgaagcagc
     1141 aatatcatca gagcctccgc attttataag ggtgactttt ttaatcttaa ggtatttttc
     1201 cagtacaacg ttcagaggtg cagtttcttc agctatttcg aattcatcct tctctttttt
     1261 gcgtatagta aaaaacttca tatttttttc aagcttggag tgtacgacga acttatcgta
     1321 atcaaccata gtgaaaacgg tatcaagatg cataaaagct cttaaagacg gtataaggac
     1381 acctattatg gtatcaaact catctccgta ttcataaaag actttttttg cgattatttc
     1441 aagtgcctca ggttcggttc tctgagatat tcctatcgca agcgtattac ggttaagaat
     1501 caggatatct ccgccttcaa ttgagtaagg catatccttt gaatagtaaa acggcgaacg
     1561 tgaaaaatcc ggatgatatt taagcacata ctccataaac attgattcac gtcttcttgc
     1621 cggagtattc atcctgttta tggtagcacc tcttcccacg actgcaaccg gatctcgctg
     1681 gaagtaaagg ttaacaagcg ggtcgagaaa gaagggatga tttagctttg attttctgac
     1741 ctggacggaa aagatgtctt ttttcagacc gagttcttca gttctcaaac cgcagaccaa
     1801 cttttcaatc atggccctag tatccatgga aacaagaaaa tcatataaag cctgggatat
     1861 gtaaggattg gttatttccg acatgtcaat gaactctcgc aaaaactcag atttaagttc
     1921 ccatgaggat aaggtctgct gcagaagatc tagcagataa acaacttcaa ccccttcatt
     1981 ccggaggatt gatgcaaact catcatgttc ctgttgcgct ttttccaagt aaggtatatc
     2041 gtcaaatagt aattcctgta agtattcagg ggctagattt tcaagttcct tggaaggtct
     2101 gtggagcatt acttttttta agtttcctat ttcagagtaa acattcagat tcatagtatg
     2161 accccctcac attaatatgg caattacata ttttactcca aataattata atttattttc
     2221 cggataatga caagtgtatt tcataattgt tataggcgga atggaatgac taacatgaaa
     2281 aaaatttcat tagataatgt ttttatgaaa atttcagcaa gcaacaaagg taataaaatt
     2341 atgataaaat taatagtcat aataaatatg ctaaggagag aggcaaaatg aaaagtataa
     2401 taaaatctgt cagcagatac tttcttttta ccaggataat gctcttatgt ttcggcatag
     2461 taatcatact cgttgtttat aagtactttc agatgaagga aatcgggaat gtatattata
     2521 ggatcgggaa tgaattttca aaaatgcttg aagaagggta tcttgcaaaa ggaaataata
     2581 taattacggt taattcttcc gacgaaattt ataagaagct tgaaagcatc ttttcttctg
     2641 tcggacagat aaacaaaact ctttctgaac cggtgagttt ggatgagata gaatattttt
     2701 tcaatcgcag ttcttttgat attgatgagc tggatcgtgc aataaggaaa gtaaaggatt
     2761 atatgtatga gtcatatctt tatgtgaata aaggttttac gtttattttc actataagtg
     2821 ttctgattgc ccttgtcata agtggaataa ggctgttttt cgagttgaaa gcaaaaaagg
     2881 tgtttgtttc atacaacgat tacgtaaaga atatgtccga aaaggttcta ggtcttataa
     2941 agcaaaatgc accttctcag aatattccaa taaggtggct tgaagaaaag gagctttatg
     3001 ataatgtttc ttcgattgaa aaggaacttg ggcttgaaaa aaggataata agcctgcctg
     3061 tttacggaac tctggaagat cttgtacctc agcttatgcc ctttctgaaa gagtatatcc
     3121 agtgtgacag agtgggggtg gcatttatcg acagtgtcga aaatgtggtg gctgagacag
     3181 cggcttctta tactgaaaat cttaaacttg gcgcaggatt tattcaaaac ataaacgata
     3241 cttcacttaa atggatagta gaaaatagat ccccaagaat aataaatgat ttggagtact
     3301 actacaaaaa ttttcataaa tcaagagcta cagaactcat tctccaggaa ggatacagat
     3361 ccagtctgac tgttccgata ataaccaacg ataaggtcgt aggctttctt tttgtcaaca
     3421 gcgttaacaa ggatgcttat accaatgaat gttcccagat aatgctaaga gttatgaata
     3481 ttcttaaggg gacgactttc tattcttata taacccagca gatagtatct acaagtgctc
     3541 aggcatttgt ttcacttgta gaaaagaaag ataatgagac aggctttcat atatcaagag
     3601 tgtcgcagta ctcatatact attgtaaatc agatgtccag agtatttccc atgattacgc
     3661 caaagttccg cagagagata ctttggtttg cttcacttca tgatatagga aagataggta
     3721 taccggacaa catacttctc aagcctggca aactcactcc tgaagagttt gatataatga
     3781 agcagcatgt gcttttcgga gaaaatattc ttaagg
//
LOCUS       DAOSHR010000190         3798 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_22184_length_3798_cov_5.406359, whole genome shotgun sequence.
ACCESSION   DAOSHR010000190 DAOSHR010000000
VERSION     DAOSHR010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22184_length_3798_cov_5.406359"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..850)
                     /locus_tag="PLU28_09170"
     CDS             complement(80..850)
                     /locus_tag="PLU28_09170"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOG35267.1"
                     /translation="MILFDYHTHTNFSDANYGFDELLEELKKTDIKIIGITDHFEKNL
                     MQSVKVSKSEYEKAFGVFRDKCFDCGITAVLGAEVGMKEKEFLPSDNLPDVSYIIASF
                     HQMPEKGSMTEKEYWEYYMNVVEYGLKNFRFDIIGHIEGYMPVTPFMHEEASFDERRS
                     TEKLFAKKYFTLEWYEKISKIMKERNIAIEIHGGSKSPRIEVLSIMKKNNVKFSFGSD
                     THSLEQLKKHREYFDYAVKALDLKETDFIKLGGEVCRK"
     gene            complement(875..1987)
                     /locus_tag="PLU28_09175"
     CDS             complement(875..1987)
                     /locus_tag="PLU28_09175"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOG35268.1"
                     /translation="MKVPLLFVTIDALGPDLVQKADMPFLKQMISQGASVKNATSCFP
                     TLTTPMMSTILTGMYPQNHGIPANYRLFFEKKEAAGTLRNLKSITISEILMNQGYKTL
                     SAQHFMLADYDGKKRCSRYIEPGGGDPQILTQAIIKAFETEKYDAVFTIYQSVDNTGH
                     SHGPLSDTNIKAAEDIDRNMKELYEFLKKEWGEFLTVVSSDHSMSRADKHSNFSIEKI
                     LEKYSLKYEFYQPGDKISNEIDCALLNFPMVPIFLMTDKARSSGEEIIKDLRNDKEVE
                     VVYDKKQMKDLGNELYGDISYSLVKGHSTSKLLLIPFAKFGYHGTVHEKPAVINYFGP
                     MVCKRQIESSELVDIVPTVLKYLGMSSEIKFDGTAK"
     gene            complement(2002..2859)
                     /locus_tag="PLU28_09180"
     CDS             complement(2002..2859)
                     /locus_tag="PLU28_09180"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOG35269.1"
                     /translation="MRKLAVILAVICLVLCSCCCNSPERLADKGSNGHTGGNGGEDVQ
                     KNTLRVMTYNIHGGKDSYDNDSMERIIELVKRIDPDVLVLNEVLGTRGFPDENQAYKI
                     AAECKMKYYFGKSINTGFMGIQEFGNAVLTKFSIKKAYKKHLFYYDNSPGYSEQRTCS
                     CVCIDYFGKDLWIYATHLGLKDIKTERGKQLDNIFEQILANGGSSILAGDMNEKWEVL
                     QKYDHKVYKNNFYSSNHKLLNKSIQTFSKSTEQIDYIFLSNDIKINSCQNFISDASDH
                     YPLVAEVEI"
     gene            complement(2856..3626)
                     /locus_tag="PLU28_09185"
     CDS             complement(2856..3626)
                     /locus_tag="PLU28_09185"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOG35270.1"
                     /translation="MRKLFFTLVVLLSILSMGATVNLKITTYNIRHGLGVDNVFNFQR
                     VKETIQKINPDILILNEVDQGNARSGDVYQAKEIAALMGMEYYFYATEGKTNYGIAVL
                     SKYPVIKAEGFNLPQPRFMKAVQRGCINMIVNVCGKELSIYGAHFGLGGFQEVQKELE
                     EIYKRYQNNPITSVIGGDFNLEWAPLRSAVPQMFKSDFKSSNHSIGKDLYTIPADSPG
                     PQIDFLLVTNDVIVKDTYTYDSWASDHLPVIAEIELEL"
BASE COUNT         1044 a          842 c          627 g         1285 t
ORIGIN      
        1 ttcacgcata tatcaaggtc tttcatattc tgataagctt ttgaatgtgt tttacccatc
       61 atgccgcagc ctattatggc tattttctgc atacttctcc tcctaatttg ataaaatctg
      121 tctctttaag gtcaagtgct tttaccgcat aatcaaagta ctctctgtgc tttttcaatt
      181 gttcaagcga atgggtatct gagccgaaag agaactttac attgttcttt ttcatgatag
      241 aaagaacctc aattcttgga gatttcgaac ctccgtgtat ttcaatggct atatttcttt
      301 ctttcattat ttttgaaatt ttctcatacc attcaagcgt aaagtacttt tttgcaaaaa
      361 gcttttcagt actcctgcgt tcgtcaaaag aagcttcttc gtgcataaaa ggcgtgactg
      421 gcatgtaacc ttctatatgg cctattatat caaacctgaa gtttttgagc ccatactcaa
      481 ctacattcat atagtactcc cagtactctt tctccgtcat actacccttt tccggcatct
      541 gatgaaagct tgctattata tagctgacat ctggaagatt atcagacgga agaaattcct
      601 tctccttcat cccgacttct gctcctaaaa cagcagttat tccacaatca aaacatttgt
      661 ccctgaaaac tccaaaagct ttttcatact cgctttttga aaccttaacg ctttgcataa
      721 gatttttttc aaagtgatcg gttattccaa ttatttttat gtcggttttt ttcagttcct
      781 ccaaaagctc atcaaaccca taattagcat cggagaaatt agtgtgggta tggtagtcaa
      841 acagtatcaa ctttatctat ctccttttac tgctttattt tgcagtacca tcaaatttaa
      901 tttcagaact cattccgaga tatttaagga ccgtcggaac tatatcaacc agttctgagc
      961 tttcaatctg tcttttgcaa accatgggtc caaagtagtt tattacggca ggcttttcgt
     1021 gtacagtacc gtgatatccg aacttcgcaa acggtatcaa aagcagttta gatgtactgt
     1081 gtccttttac caggctgtag ctgatatctc cgtaaagctc atttcccaga tctttcatct
     1141 gttttttatc gtatacgacc tctacttctt tatcgttacg aagatcttta ataatttctt
     1201 ccccggaact tctggcttta tccgtcatca ggaatatagg aaccatagga aaattaagaa
     1261 gggcacaatc aatctcattg cttatcttat ccccgggctg ataaaactcg tacttcaaag
     1321 agtacttttc aagaattttt tctatggaaa agttagaatg tttatcggct ctgctcatgg
     1381 aatgatcaga ggaaactaca gtcagaaact ccccccattc ttttttcaaa aattcataaa
     1441 gctctttcat attcctatct atatcttcgg ctgctttaat gttggtatcg gaaagcggac
     1501 cgtgtgagtg tcccgtatta tccaccgatt gatatatcgt aaagactgca tcgtactttt
     1561 ctgtttcaaa agccttaatt atagcttgtg taagaatctg aggatctcct ccgcccggct
     1621 caatgtatct actgcatctc ttttttccat cataatcggc aagcataaaa tgctgagcag
     1681 ataaagtttt gtatccttga ttcataagta tctctgaaat agttatgctt ttcaagttcc
     1741 taagtgtgcc tgcagcctcc tttttttcaa aaaaaagtct gtagttggcc ggaataccgt
     1801 gattctgcgg atacataccc gtgagtatgg tactcatcat aggcgttgta agcgtaggga
     1861 aacaggacgt tgcatttttc acgcttgcac cttggcttat catctgctta agaaagggca
     1921 tatcggcttt ctgaaccaga tccggcccca gggcatctat cgtaacaaac aataacggta
     1981 ctttcattat tttcatccct tttatatctc aacttcagca acaagaggat aatgatcgga
     2041 agcatcagat ataaagtttt gacagctgtt gattttgatg tcgttgctca gaaatatgta
     2101 atctatctgc tccgtgcttt ttgaaaatgt ttgtatgctt ttgttgagta gtttatggtt
     2161 cgatgagtaa aaattattct tgtagacctt atgatcatat ttttgaagta cttcccattt
     2221 ttcgttcata tcaccggcta aaattgaact tccgccgttt gccaatatct gctcaaatat
     2281 attatcaagc tgcttcccac gttcagtctt gatatccttt agtccaagat gggtagcata
     2341 tatccaaaga tctttgccaa agtaatctat gcatacgcac gaacatgtcc tctgctcact
     2401 gtatcccgga ctgttgtcat aataaaaaag atgcttcttg tatgctttct ttatactgaa
     2461 ctttgttaaa acagcattac caaactcctg gatgcccata aaacctgtgt ttatactttt
     2521 gccaaaatag tacttcatct tgcactctgc ggcaatttta tatgcctggt tttcatccgg
     2581 gaaaccccta gttcccaaaa cctcgttaag cacaagcaca tcaggatcta tcctttttac
     2641 aagctctata atcctttcca tagagtcatt gtcataagaa tcttttccac catgtatatt
     2701 atatgtcatt actcttagcg tatttttctg cacatcttca ccaccgtttc cgcctgtatg
     2761 tccatttgaa cctttatctg caagacgttc cggagagtta cagcaacagc tgcagagcac
     2821 aagacaaatc acagccaaaa ttactgccag tttcctcata attcaagttc tatctccgca
     2881 attaccggaa gatggtctga agcccatgaa tcataagtat aggtatcttt cactataaca
     2941 tcattggtca ccagaagaaa gtctatctgc ggaccgggac tgtcagccgg aattgtgtat
     3001 aagtctttac ctatagaatg gtttgatgat ttaaaatcac ttttaaacat ctgtggaaca
     3061 gctgaacgca aaggtgccca ttcaaggttg aagtctccac ctattaccga ggttatagga
     3121 ttattctgat accttttgta tatttcttca agttccttct gaacttcctg aaaacctccc
     3181 agaccaaagt gtgcgccata tatgctcagt tcttttccgc aaacgttgac aatcatattt
     3241 atacagcctc tctgaaccgc cttcataaaa cgcggctgag gcaaattgaa gccttcggct
     3301 tttatcacag gatactttga caaaacggct atcccataat tggtcttacc ttcggttgcg
     3361 tagaaataat actccatacc cataagagct gctatttcct tagcctggta tacatcaccg
     3421 cttcgtgcat tcccctgatc cacttcattg agtataagaa tatctgggtt gatcttctgt
     3481 atagtctctt taactctctg aaagttgaaa acattatcaa ctcccagtcc atgtcttata
     3541 ttatatgttg ttattttaag atttactgtt gcacccatgg aaaggatgct caataaaaca
     3601 actaatgtaa aaaaaagttt tctcatacaa actcctccta ttcagccata gcttgcaaaa
     3661 ttctatcgtg aagctccgaa agatggtact taccgttgaa cagctgagaa ttcggcccct
     3721 tggcaagaat cggtatatat gcagcggtat ggtctcctat agtccagcct attttaatgt
     3781 ttgttccgtc attcgctc
//
LOCUS       DAOSHR010000191         3787 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_22273_length_3787_cov_5.643623, whole genome shotgun sequence.
ACCESSION   DAOSHR010000191 DAOSHR010000000
VERSION     DAOSHR010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 3787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3787
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22273_length_3787_cov_5.643623"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /locus_tag="PLU28_09190"
     CDS             <1..724
                     /locus_tag="PLU28_09190"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOG35271.1"
                     /translation="TRTIDDSKRKEMYSFVREELNNNNKAFFVYPLIEESEKVDLKSA
                     QEMYSSELKNEFSDYGVGIIHGKMSSSEKNEIMENFSKGSINVLVSTTVIEVGIDIPQ
                     ATVMVVEHAERFGLSQLHQLRGRVGRSNRQSYCFLIISDNTGDETKQKIIEFASTTDG
                     FKVADMDLAWRGPGKFFDIQQHGIDEFKFIDLSQDILLISSIRNDISNLLSDDPNLQK
                     YPDLKKEVYAKYGKKLKLIKAI"
     gene            735..1301
                     /locus_tag="PLU28_09195"
     CDS             735..1301
                     /locus_tag="PLU28_09195"
                     /inference="COORDINATES: protein motif:HMM:NF015559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HOG35272.1"
                     /translation="MALKIETGSLRGKSFSPVDDKRTRYTTGQLRMSIMNMFDFNGAI
                     IFDFFGGSGSFSFEAISNGAESSYIIDISKKAVSTIIRNAKTIGIDNKITVLNIDYSK
                     AIEKFLSENTTADVIFADPPFNLGFCSELLQHIDSNPDILNDNGFILLEKSDKEEINY
                     SCNCFILEQKRNYGDIELLIYTKKNRHE"
     gene            complement(1382..2770)
                     /locus_tag="PLU28_09200"
     CDS             complement(1382..2770)
                     /locus_tag="PLU28_09200"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOG35273.1"
                     /translation="MYEKSKVIGLNLKVVVIFLILTLFSTVALSALPVYVQWRELDPK
                     NVRVENDITYIQLKEVAGKFGKVIDVENEALVLIKHDKWKVFLFPKSGMCVINNLESF
                     NIQETDARFYDGSIFLSPQLLAKILDLELIVSSKGIYFNRSLSKVQSIKTIIQKSLVR
                     TIIELSEDAESEISPLVSTYGYLIKIFGAAVPETFYYEEFNDKIKYIKAYHYSETEVW
                     VKIVLNYKAQVNKIIEDKRIVLDFTFEEVDEPVVVIDAGHGGYDPGTVGFSKLYEKDV
                     ALAVAQKAKELLKEYSIDVKLIREDDTYIDLHKRATMSNDFEADLFISVHLNSFPDDR
                     TVRGSEVYYFDFSKTDYARKIAWRENLDQKKDKQLIETWVHDKESSISASKKFGEVLE
                     SNLKLKGIKSRGVSPAEFAVLAYTRSPAVLFELEFLSNNVVEENFKNSKYTKLFAEII
                     ADSVIKYFDLKK"
     gene            complement(2787..3629)
                     /locus_tag="PLU28_09205"
     CDS             complement(2787..3629)
                     /locus_tag="PLU28_09205"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOG35274.1"
                     /translation="MMIRKVLLSIIICACMLSFSAAGYTYEIKSGDTVYSILKSTGIS
                     LGVLMDYNQSLSESKYLIVGKKLFIPSENSFSYKVVAGDNLSTIAQKYFTHPDLLATY
                     NNIKNSQIIRSGQELKIPYKFVGACINKASNIVWPLVGYITSEYGYRKHPIDGITKFH
                     SGIDIAAPSGTPIVSASNGMVIDVGCDDGYGKFVKVSSGARVYMYAHLNSMNVVKGYA
                     VKQGEIIGRVGSTGISTGPHLHFQVEDLKGKTYDPGVYLDNIKYAYNLDYIPEEFKTY
                     MGGK"
     gene            complement(3626..3784)
                     /locus_tag="PLU28_09210"
     CDS             complement(3626..3784)
                     /locus_tag="PLU28_09210"
                     /inference="COORDINATES: protein motif:HMM:NF013502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HOG35275.1"
                     /translation="MKHYGGKVIAESQETCVVYGMPKAVVEEGYADYILPNYKISEKI
                     TDILEGRG"
BASE COUNT         1220 a          757 c          603 g         1207 t
ORIGIN      
        1 tacaagaact attgacgatt caaagcgcaa agaaatgtac tcttttgtac gggaagaact
       61 taacaataat aataaagctt tttttgtcta ccccttgatt gaagagtcag aaaaagttga
      121 tctaaaatca gcgcaggaga tgtactcatc agaattgaaa aacgaatttt ctgattacgg
      181 cgttggaata atccatggaa aaatgtcatc ctctgaaaaa aacgaaataa tggaaaattt
      241 ttccaaagga agtataaacg tactggtaag cactacggta attgaagtcg gtatagatat
      301 cccacaggca accgttatgg ttgttgaaca cgcagagaga ttcggtttat cacagcttca
      361 tcagttacgc ggaagagttg gcagaagtaa ccgtcaatcc tattgctttt taatcatatc
      421 agacaacacc ggggatgaaa ctaaacaaaa aataattgag tttgcttcta ctaccgacgg
      481 tttcaaagtg gcagatatgg atctggcatg gagaggtccg ggaaagttct ttgatattca
      541 gcagcacgga atagatgaat ttaagtttat tgatctttca caggatatct tgcttatatc
      601 ttccataaga aacgatatat ctaatttact atccgatgat cccaatctgc agaagtatcc
      661 ggacttgaaa aaagaggttt atgcaaagta cggaaaaaag ctaaaactaa taaaagctat
      721 atgaggtgat tattttggct cttaaaatag aaacaggatc acttagaggt aaaagttttt
      781 cccctgtgga tgacaaacgt actcgatata ctacaggaca gcttagaatg tctattatga
      841 atatgtttga tttcaatgga gcaattattt ttgatttttt cggaggttct ggctcttttt
      901 cgtttgaagc tatcagcaat ggagcagaga gttcttatat tatagatatt tctaaaaaag
      961 ctgtttcaac tataataaga aacgctaaaa ctatagggat agataataaa attacagttt
     1021 tgaacatcga ttattccaaa gctatagaaa aatttctttc tgaaaataca acggctgacg
     1081 ttatttttgc cgatccccct tttaaccttg gattttgcag tgaactttta cagcatatag
     1141 actctaatcc tgatatactt aacgacaacg gatttattct acttgaaaaa tctgacaagg
     1201 aagagattaa ctattcctgc aactgcttta tccttgagca aaaaagaaac tatggcgata
     1261 tagaacttct catatacacc aaaaaaaaca ggcatgaatg aagtgaaccc ctttagttag
     1321 attctgtcta actaaagggg ttcacttcaa ataacgtatg cctgtaatta tttttattat
     1381 tttacttttt taaatcgaaa tactttataa ccgaatccgc tattatttca gcaaaaagct
     1441 ttgtgtactt tgagttctta aaattttcct caactacatt gttacttaaa aattcaagtt
     1501 caaaaagtac agctggactt ctggtatatg ccagaactgc aaactcggcc ggagatactc
     1561 ctcttgattt tataccctta agcttgagat tactttccaa aacctctccg aacttcttac
     1621 tagcacttat agagctttct ttatcgtgaa cccaagtttc tataagctgc ttatcttttt
     1681 tctgatcaag attttcccgc caggctatct ttcttgcgta gtcagtttta gaaaaatcaa
     1741 agtagtatac ctcacttccc ctcacagttc tatcatctgg aaaagaatta aggtgaacac
     1801 ttataaaaag atcggcttca aaatcatttg acatcgtagc cctcttatga agatcgatgt
     1861 aagtatcatc ttctcttatg agcttaacat caatactgta ctccttcaaa agttctttcg
     1921 ccttctgagc aacagccagt gcaacatctt tttcatacaa cttcgaaaaa cctacagttc
     1981 ccggatcata tccaccatgt ccggcatcga ttactaccac cggctcatca acttcttcaa
     2041 aagtaaaatc aagaactatt ctcttgtctt caataatctt attcacttgg gctttataat
     2101 taagaactat cttaacccat acctctgttt ccgaataatg ataagccttt atgtacttta
     2161 tcttatcgtt aaactcttca taataaaatg tttccggcac agctgcaccg aatatcttta
     2221 taagataacc ataagtgctt accaatggtg agatctctga ttcagcatct tcagaaagct
     2281 caataatagt ccttacaagc gatttttgta ttatagtttt tatcgactga acttttgata
     2341 aagatctgtt aaaatagata ccctttgagc ttactataag ctcaagatca agtattttag
     2401 caagaagctg aggagaaaga aagattgaac cgtcatagaa ccttgcgtca gtttcctgaa
     2461 tgttgaacga ttcaagatta tttattacac acattccact ctttggaaaa agaaagactt
     2521 tccatttatc atgttttata agcacaagag cttcgttttc gacatcaata acttttccaa
     2581 actttccggc tacttctttc aactgtatat aagttatatc attttcaacc ctaacattct
     2641 tcggatcaag ttctctccat tggacatata ccggaagggc acttaatgct acagtcgaaa
     2701 aaagagtcaa aatcaaaaaa ataactacta ccttcagatt cagccctatg actttacttt
     2761 tctcatacac cacattcacc ttctaattat ttaccaccca tataggtttt gaactcttcg
     2821 ggtatgtaat caagattata cgcatacttt atattgtcaa gataaactcc cggatcatat
     2881 gttttacctt taagatcttc aacctggaag tgaagatgag gcccggtgga tatccctgta
     2941 ctgcctactc ttcctataat ctcaccttgc ttaacagcat agccctttac aacgttcatg
     3001 gagttcaaat gtgcatacat atagactctg gctcctgaag atacttttac aaattttccg
     3061 tatccgtcat cacatccaac atcaataacc ataccgtttg aagctgatac aataggtgtt
     3121 ccagaaggag ctgcaatatc aatacctgaa tggaacttgg ttataccgtc tatcggatgt
     3181 ttcctgtagc catattcgga ggttatataa cctacaagag gccatacaat attggatgct
     3241 ttattaatac atgctccgac aaatttgtat ggaatcttta attcctgtcc agatcttatt
     3301 atctgagagt tttttatgtt attatacgtt gccaagagat ccggatgcgt aaagtacttc
     3361 tgggcgatcg tagacagatt atcaccggca actaccttat aagaaaatga attttcacta
     3421 ggtataaaaa gcttcttccc gacaataaga tacttacttt ctgaaagcga ctgattgtag
     3481 tccatcagta caccaagcga tattcccgtg cttttaagaa tagaataaac agtatctcca
     3541 ctcttgatct catatgtgta tcccgcagca gaaaaagaaa gcatacaggc acatatgatt
     3601 atacttaaca gaactttcct gatcatcatc cacgcccctc tagaatatct gtaattttct
     3661 cagagatctt atagttggga agtatgtagt ctgcgtaacc ttcttccacc acagcctttg
     3721 gcatcccata aactacacat gtctcctgag attcagcaat tacctttccg ccataatgct
     3781 ttatttt
//
LOCUS       DAOSHR010000192         3775 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_22389_length_3775_cov_6.199194, whole genome shotgun sequence.
ACCESSION   DAOSHR010000192 DAOSHR010000000
VERSION     DAOSHR010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 3775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3775
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22389_length_3775_cov_6.199194"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="PLU28_09215"
     CDS             <1..404
                     /locus_tag="PLU28_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35276.1"
                     /translation="TVYGLDTDVDEYIQREGSQGIRKLIKENTYSWSEYYAAFSPALK
                     KKNQYDQIIKTMAMEAPEKFENLSKILSQSWQICHKEYKNEQEIKQDLIHQKTLLINE
                     KKKKLETEICYYQQEMKELLNQQLELIQEVR"
     gene            406..1377
                     /gene="cas1b"
                     /locus_tag="PLU28_09220"
     CDS             406..1377
                     /gene="cas1b"
                     /locus_tag="PLU28_09220"
                     /inference="COORDINATES: protein motif:HMM:TIGR03641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated endonuclease Cas1b"
                     /protein_id="HOG35277.1"
                     /translation="MESIKIFSSGELKRHDNTLVLITKDQKKSIPVENVEDIKVFSEI
                     ILNKRTLEFLTKKNITMHFFNFVGNYIGTYYPLEFNQNGEVFLKQAEHYVELNKRMEL
                     ARSFVVGEMMNLEYNLQTSGIKKESIKISPKILQIEKSRTVEELMIVEAGIRQEYYKE
                     FDKLTNNPDFKFEKRTKQPPKNEVNALISFGNCMMYSDILKCIFKSHLDPRIGYLHST
                     NQRGFTLNLDISEIFKPIVVDQTIFHITGQRLLTKKDFRTFENGVYLNEEGRKKFITE
                     YEKQLKKTVFSREKGRYMSYKLLMQSEVYKIENHLLQIKPYKPYIHI"
     gene            1397..1579
                     /locus_tag="PLU28_09225"
     CDS             1397..1579
                     /locus_tag="PLU28_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35278.1"
                     /translation="MAAIIALIFLGAIISGIKEIFFSSPEVKTHTQNKPQENRELTIK
                     EKNISVLTKKTTTYLV"
     gene            1594..2196
                     /locus_tag="PLU28_09230"
     CDS             1594..2196
                     /locus_tag="PLU28_09230"
                     /inference="COORDINATES: protein motif:HMM:NF025840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DarT ssDNA thymidine ADP-ribosyltransferase
                     family protein"
                     /protein_id="HOG35279.1"
                     /translation="MYKEVVERKGYLIHVTERKNLAGILECKNILSLQNLKKNDIKPA
                     FMTDEISRNLDHKKGFDEYVHLAFDESYPMFSAKLYYLQLNDPAIIKIDPKIITDHEK
                     DILLSDTNSSASLAKTGSINELLSSLDFQIIYFPKDRITNTNIYQNLKKKKQSEILVK
                     EKIPLEYVKEIYVSTAKGLNITDNMKITETDTCKKLRLNF"
     gene            2212..3501
                     /locus_tag="PLU28_09235"
     CDS             2212..3501
                     /locus_tag="PLU28_09235"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HOG35280.1"
                     /translation="MLEDNIKGMMIGAAIGDALGYPLEIMGNKNTDGTKIKSVQTFTD
                     WTRVHKNRYFKCTEEIKAGTYSDDTQLTLCVSRSYKNGVFSPQTMALELSEWLAYQTG
                     GGRAVKAAAKNINFKNADESIWYKNFYEGYTYSGGNGALMRITPHIAANVKNKEFTAG
                     LKNAIKDCLITHGHPHAIIAQIFYFTFLWVLTNKLPAEKAFKVAKEECTKRSLKNIGL
                     LPDEWFEKYLENNGCSYEKIEEITMKEFLNKALIVENAIKSNMPEKNLYEKLQATGKF
                     KGSGINTALAAAYTVIKNDRSSFAETVLIPANIFQCDTDTTACVAGAAKGLMLGLAGI
                     EKTLVSTLQDTEYIKLCAQALYNGSGHHAFQLSLTDKPLEYYRKNLTEINHGEVLKTR
                     LFGSAVIVEKNMNETLLTARIKTQNPEFTLIIKKIPK"
BASE COUNT         1549 a          661 c          629 g          936 t
ORIGIN      
        1 ataccgtata cggactggat acagatgtag atgaatatat tcagcgtgaa ggatcgcagg
       61 gaataagaaa actcataaaa gaaaatacct atagctggtc agaatactat gcggcattca
      121 gtcctgcttt aaagaaaaaa aatcaatatg atcaaataat aaaaaccatg gcaatggaag
      181 caccggaaaa gtttgaaaac ctttcaaaaa tactttctca aagctggcaa atatgccata
      241 aagaatataa aaacgaacaa gaaataaaac aagatctcat ccatcaaaag acattgctca
      301 taaacgaaaa gaaaaagaaa cttgaaacag agatatgcta ttaccagcaa gaaatgaaag
      361 aactacttaa tcaacagctg gaactcatac aagaggttag ataaaatgga aagcataaaa
      421 attttttcat ccggagagct taaaagacat gacaatacac tggttttaat cacaaaagat
      481 caaaaaaaga gcatccctgt agaaaatgtt gaagatataa aagtgttttc agagattatc
      541 ctaaataaaa gaactttaga atttttaacc aaaaaaaaca taacaatgca ttttttcaat
      601 ttcgtaggga actacatagg tacatactat ccccttgaat tcaatcaaaa cggcgaagta
      661 tttcttaaac aagccgaaca ctatgttgaa ctgaacaaaa gaatggaact tgcaagatca
      721 tttgttgtag gagaaatgat gaaccttgag tacaaccttc aaacttcagg tattaaaaaa
      781 gaaagcatta aaataagccc caaaatactc cagatagaaa aaagccgtac tgtagaagaa
      841 ctaatgattg tggaagccgg tataagacaa gagtattaca aagagtttga taagcttacc
      901 aacaatcccg acttcaagtt tgaaaaaaga accaaacaac ctccgaaaaa cgaggtaaat
      961 gcgcttataa gctttggcaa ctgcatgatg tactcagaca tactaaaatg cattttcaaa
     1021 tcacatcttg atcctagaat cgggtacctt cactcaacaa atcaaagagg ctttacactt
     1081 aacctggaca tatcagagat attcaaacct atagttgtcg atcaaactat atttcacata
     1141 accggtcaaa gattactaac caaaaaagat ttcagaacct ttgaaaacgg agtgtacctt
     1201 aacgaagaag gcaggaaaaa attcattacc gagtatgaaa aacaactcaa aaaaacagta
     1261 ttttcaagag aaaaaggaag atacatgagc tacaaacttc ttatgcaaag cgaagtatat
     1321 aaaattgaaa accatcttct acaaataaaa ccttacaaac cctacatcca catttgatac
     1381 aggaggtgtt gcataattgg ctgcgataat agccttgatt tttttaggag cgataatttc
     1441 tgggataaaa gaaatttttt tctcctcacc agaagttaaa actcatacgc aaaacaaacc
     1501 ccaagaaaac agagaactaa ctattaaaga aaaaaatata tcagttctta caaaaaaaac
     1561 gacaacttat ttggtataaa caaatcagaa tacttgtata aagaagtggt tgaaagaaaa
     1621 ggttatctta tccatgttac agaaagaaag aatctagccg gaattcttga atgcaagaac
     1681 atattatccc tgcaaaacct aaaaaaaaat gatattaaac ccgcttttat gacggatgaa
     1741 atatctagaa atcttgatca taaaaaagga tttgatgaat atgtacacct tgcttttgat
     1801 gaaagttatc ccatgttctc agccaaactt tactacttac agctaaatga tcctgcaata
     1861 ataaagatag accctaaaat aattacggat cacgaaaaag acattcttct ttcagataca
     1921 aactccagtg cctctttggc aaaaaccggc tccataaatg aacttctctc ttcacttgac
     1981 tttcaaataa tttactttcc taaagatcga ataacaaaca caaacatata ccaaaatctt
     2041 aaaaaaaaga aacagtcaga gattcttgtc aaagagaaaa ttcctcttga gtatgtaaaa
     2101 gaaatctatg tatcaaccgc aaaaggacta aatattacag acaacatgaa aataaccgaa
     2161 accgatacat gcaaaaaact aagactgaac ttttaaaaag gtggtgagct gttgttggaa
     2221 gataatataa aaggaatgat gataggagca gctataggag atgccttggg atacccgtta
     2281 gagattatgg gaaataaaaa tacagacggt actaaaataa agagcgttca aacctttact
     2341 gactggacaa gagtacacaa aaacaggtac ttcaaatgta cagaagaaat aaaagccggc
     2401 acatactcag acgataccca gctaacactc tgtgtttcaa gaagctataa aaacggagtt
     2461 ttcagtcccc aaaccatggc tcttgaactc agtgaatggc tggcatatca gacaggaggc
     2521 ggaagagctg tcaaagccgc cgccaaaaat ataaacttta aaaatgcaga tgaaagtata
     2581 tggtataaaa atttttacga aggctacacc tattcaggag gaaatggtgc attgatgaga
     2641 attacacctc atattgcagc taatgttaaa aacaaagagt ttaccgccgg tcttaaaaat
     2701 gcgataaaag actgccttat tactcacgga catccacatg ccatcatcgc ccagatattc
     2761 tactttacat tcttatgggt tctcacaaat aaacttccgg cagaaaaagc atttaaagtt
     2821 gcaaaagaag agtgcaccaa aaggagcctg aaaaatatag gactacttcc cgatgaatgg
     2881 tttgaaaagt atcttgaaaa taacggttgc tcttacgaaa aaattgaaga gataaccatg
     2941 aaagagtttt taaacaaagc acttattgta gaaaatgcca ttaagagtaa tatgcccgaa
     3001 aaaaatctgt atgaaaaact ccaggctacc gggaagttta aaggctcagg cataaacacg
     3061 gcacttgcag ctgcctatac agtcataaaa aatgacagat caagcttcgc tgaaacagtt
     3121 ttaattcctg ctaacatatt ccagtgtgat acagacacaa cagcttgcgt tgccggtgcc
     3181 gctaagggat tgatgctagg actggctgga attgaaaaaa cacttgtaag cacactgcaa
     3241 gacactgaat atataaaact ttgcgcacag gcgctttaca atggatctgg acatcatgct
     3301 tttcagctat cccttacaga caagccgctg gagtattaca gaaaaaattt aacagaaata
     3361 aaccatggtg aagtattaaa aaccagactt tttgggtctg ctgtaatagt agaaaaaaat
     3421 atgaatgaga cgcttctgac agctagaatc aagacccaaa accccgagtt cacattaata
     3481 attaaaaaga taccaaaata aaaaatataa taataattaa cttatcaact gatataaaaa
     3541 taaatgtaga tttaaaaaaa gaaataaaaa tgatataatt atatttgaag cataacaaaa
     3601 aattttgaaa ggagaaaagc atgtctatac ttacagtcta tgatgtaaca ggtattcaga
     3661 agtttatttt cagttcttcc aaacttaagg aaaattttgg aggctcactt cttgtagcca
     3721 gagtaatgga agaacttctt gtaaacgcag taaagcaagc caattcaaaa gctta
//
LOCUS       DAOSHR010000193         3762 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_22514_length_3762_cov_5.265444, whole genome shotgun sequence.
ACCESSION   DAOSHR010000193 DAOSHR010000000
VERSION     DAOSHR010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 3762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3762
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22514_length_3762_cov_5.265444"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1111
                     /locus_tag="PLU28_09240"
     CDS             <1..1111
                     /locus_tag="PLU28_09240"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG35281.1"
                     /translation="SCIIAGVLGDRLNRKHIMVNMDFARGILIFIMSVVSIYGGLNIT
                     VLIIGQIILAVLDAIFNYSTGSVMLPDLVRSGDLRKANSLVSSFDWTARILGPAVGGL
                     IYSAVGISGVFLINAVSFVLSAISEIFIVYKRPVIRDKKLSVKIFFEDIKEGLGYIKK
                     SQNLMILFLFAMFINFMSTPMFSVVIPFVMRSTIKFDAVQYGAVEMFFSVGILLGNFA
                     LVTILSKKESKKIIKSGLFFQTIYFVATSVLMFPAVIMFLNGPSVTMFGLFSLSMAIM
                     GFFNALINTPLMTIMQKLIPADMRSRVFSVLDFMGQIAVPVGIFIVGMLSDKIGVHYI
                     YLVDNIIVFFVVMLFIFSGKEEILSDEPSPVQASK"
     gene            1218..2471
                     /locus_tag="PLU28_09245"
     CDS             1218..2471
                     /locus_tag="PLU28_09245"
                     /inference="COORDINATES: protein motif:HMM:TIGR03916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA modification/repair radical SAM
                     protein"
                     /protein_id="HOG35282.1"
                     /translation="MDIVEKLKILSQSAKYDASCASSGSERENSGGMGNASCAGICHS
                     WADDGRCISLLKVLFSNQCMYDCAYCVNRKSNDVRRASFTQEELVQLTMNFYKRNYIE
                     GLFLSSAVFVSPDVTMEHMLGVVKRLREVEKFYGYIHLKAIPGADLSIIRQAGMYADR
                     MSVNIELPSESSLKMLAPDKKKDDILKPMVFMGKNWLESTQERQKYRKAPLFIPGGQS
                     TQLIVGATPDNDLQIIKLAEGLYKKFSMKRVYYSAYIHTNSSDDRLPVLAYPPLRREN
                     RLYQADWLLRFYKFSANEILDSSFPYLDEEIDPKSSWALRHMEFFPLEVNRAGYDELL
                     RVPGMGVKSILKILNARRYGKIMPDDLKKFGVVLKRARFFITCNGRKISDLCTDPFII
                     RAFLMSKAENKPKKLKLPFLYEVGT"
     gene            2468..3211
                     /locus_tag="PLU28_09250"
     CDS             2468..3211
                     /locus_tag="PLU28_09250"
                     /inference="COORDINATES: protein motif:HMM:TIGR03915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03915 family putative DNA repair protein"
                     /protein_id="HOG35283.1"
                     /translation="MKVFLYDGSFEGMLSTFFHILKSRESFCSIEKSGLDRPVLFSES
                     EFIPTDNLNAQYIAKAICSKISVFSFQNIIYSFLSEKNRIEDLLFRYIICGFRIGADT
                     DRHLSNKTVYSVNEISRKVSFECHKLKGLLRFSELRDGSLYAPVEPDYNIVSILSKHF
                     ERRLCNEVWMINDVKRGHAVFYRNHNLEYLEVKDIEITKNTYSEQESTYRQLWKAYFE
                     SIAIRERTNPKLQRAFMPQRYWKYLTEKN"
     gene            complement(3208..>3762)
                     /locus_tag="PLU28_09255"
     CDS             complement(3208..>3762)
                     /locus_tag="PLU28_09255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOG35284.1"
                     /translation="TPIRIVGSHTDISDWKIREEELRKCATTDSLTNAYNRLYGLNFL
                     ARQMQMSINNGEKLTICYIDVNDLKKINDTYGHGEGDELIINIINILRSSIRSSDIIS
                     RLGGDEFIVIFPKTDLITAYEIRKRIHVNLQKYNFISQKNYKISFSMGFIEYDHQKHI
                     SPENLISEADKEMYKDKFTYKTIL"
BASE COUNT         1120 a          620 c          781 g         1241 t
ORIGIN      
        1 ttcgtgtata attgcgggtg ttttgggaga tcggttgaac agaaaacata tcatggttaa
       61 tatggacttt gcacgaggca tacttatatt tattatgtcg gtagtaagta tctacggcgg
      121 tcttaatata acggttctta taatcggaca gataatactt gctgttttgg atgccatatt
      181 caattattcg acaggttctg taatgcttcc cgatctggtg cgttccggtg atcttagaaa
      241 ggctaactct ttggtaagtt cctttgactg gacggcaagg attctcggtc ctgcggtcgg
      301 aggccttata tattcagcag tcgggataag tggagttttt cttataaacg cagtttcttt
      361 cgtgctttct gccataagtg aaatattcat agtttacaaa agaccggtta tcagagataa
      421 aaaacttagt gtaaagatat tttttgaaga tataaaggaa ggactgggtt atataaagaa
      481 gtctcaaaat cttatgatac tttttttgtt tgctatgttt ataaacttta tgagtacccc
      541 gatgttttca gtggtaatac cttttgtaat gcgaagtaca attaagtttg atgcagttca
      601 gtatggtgca gttgaaatgt ttttttctgt aggtatcctt ttaggaaact ttgcgctggt
      661 aactatactt tcaaaaaagg aaagcaaaaa aattataaag tcaggactgt tttttcagac
      721 catttatttt gtagcaactt ccgttcttat gttcccagcg gtcattatgt ttttaaacgg
      781 tccttcagtt acaatgtttg gcttgttttc actttcaatg gcaataatgg gctttttcaa
      841 tgctcttata aatacaccac ttatgaccat tatgcaaaag cttattcctg cagatatgcg
      901 ttccagagtt ttttctgttc ttgattttat ggggcagata gccgtaccgg ttggtatatt
      961 tatagtagga atgctttctg ataaaattgg agttcattat atttatctgg tagacaatat
     1021 cattgtgttt tttgtagtta tgcttttcat attttctggg aaagaagaaa ttctttctga
     1081 tgagccttcc ccggttcaag caagtaaata gactttccca ataaacccct gcatttgcag
     1141 gggttgactt ttttaaataa ataaggtata cttaattata ccataaaaga aaaacaggga
     1201 taaaaagcag ggataatatg gatatcgttg aaaagctaaa aattttatca cagtctgcaa
     1261 agtatgatgc atcatgcgct tcatccggaa gtgaacgtga aaattccgga gggatgggga
     1321 atgcaagctg tgccggtata tgccatagct gggcagacga cggcagatgc atttcacttt
     1381 tgaaagtgct tttttccaac cagtgtatgt acgactgcgc ttactgtgta aaccgaaaga
     1441 gcaatgatgt cagaagggca tcctttaccc aagaggagct tgtacagctt acaatgaatt
     1501 tttataagcg aaactacata gaagggcttt tccttagctc tgccgtattt gtcagtcctg
     1561 atgttaccat ggagcatatg ctgggtgtag tcaaaaggct gcgtgaggtt gaaaagttct
     1621 atgggtatat tcatcttaaa gctattcccg gtgcagatct ttctattata agacaggcgg
     1681 gcatgtatgc cgacagaatg agtgttaata tcgaacttcc ttctgaaagc agtttgaaaa
     1741 tgcttgctcc ggataaaaaa aaggatgata ttcttaagcc aatggttttt atgggtaaaa
     1801 actggctcga aagtacacag gaacgtcaaa aatatagaaa ggctccgctt ttcatacccg
     1861 gaggacagag tacacagctt attgtaggag caacgcccga caatgatctt cagataataa
     1921 aactggccga aggtctttat aagaagttca gtatgaagcg ggtgtactac tcagcctata
     1981 ttcataccaa cagtagcgat gataggcttc ctgttcttgc atatccgcca cttaggagag
     2041 aaaataggct ttatcaggca gattggctct tgagatttta caaattcagt gccaatgaaa
     2101 tacttgacag ttcatttccg tatcttgatg aagaaattga tcccaaaagc tcttgggcac
     2161 taaggcatat ggagttcttc ccgttggagg taaacagggc tggatatgac gaactgttga
     2221 gagtcccggg aatgggggtt aaaagtatat taaaaatact caatgcaaga agatacggta
     2281 agataatgcc tgatgacctt aagaaatttg gtgtagttct gaaacgggca agatttttta
     2341 ttacatgcaa tggcagaaaa atttctgatc tttgcacaga tccctttatt atcagagctt
     2401 ttcttatgtc taaagccgaa aacaagccca aaaagcttaa acttcctttt ttatacgagg
     2461 tgggtacatg aaagtttttc tatatgatgg ctcatttgaa ggtatgttaa gcacattttt
     2521 tcatatactc aaaagcagag aaagtttttg ttcgattgaa aaatcaggat tggacagacc
     2581 tgtacttttt tcagaatcag agtttattcc cactgataac ttaaatgccc agtacatagc
     2641 aaaagccatc tgttcaaaaa tctctgtctt ttcatttcag aacattatat actcttttct
     2701 ttcggaaaag aatagaatag aggatctttt attcagatac attatctgcg ggttcagaat
     2761 aggtgcagat acggataggc atctttctaa caaaacagtt tacagcgtta atgagataag
     2821 caggaaggta agttttgaat gtcataagct taagggcttg ctaagatttt cggagcttag
     2881 ggatggcagt ctttatgcac cggtggagcc tgactataat attgtttcta ttttatcaaa
     2941 acattttgaa agaaggcttt gcaacgaggt ctggatgata aatgatgtaa aaagaggaca
     3001 tgctgttttt tacagaaacc ataatcttga atatttggaa gtaaaggaca ttgagataac
     3061 taaaaatact tactccgagc aggagagtac ttatcggcag ttatggaagg catactttga
     3121 aagcatagcc ataagagaga gaaccaatcc aaaacttcaa agggctttta tgcctcaaag
     3181 atactggaag tatcttacgg agaagaacta aagaatagtt ttgtaggtga atttatcttt
     3241 atacatctct ttgtctgctt ctgagatgag attttcagga gatatgtgtt tttgatggtc
     3301 gtattctata aagcccatgc tgaagctgat tttgtaattt ttctggctta tgaagttata
     3361 cttctggagg tttacatgta ttctttttct tatttcatat gctgtaataa gatctgtttt
     3421 agggaaaatt actataaatt catctccacc caaccgtgaa attatatcgc tgctacggat
     3481 gcttgatctg agtatgttta taatatttat aattaattcg tccccttctc cgtgaccata
     3541 ggtatcattt atttttttga gatcattcac atctatatag catatggtga gcttttcacc
     3601 gttatttatg gacatttgca tctgccgtgc cagaaagtta agaccgtata atctgttata
     3661 tgcattggta agcgagtctg ttgtggcgca tttccttaac tcttcctcac gtatcttcca
     3721 atcggagata tcagtatggc ttcctacaat tcttatcgga gt
//
LOCUS       DAOSHR010000194         3717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_22879_length_3717_cov_6.402512, whole genome shotgun sequence.
ACCESSION   DAOSHR010000194 DAOSHR010000000
VERSION     DAOSHR010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 3717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3717
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22879_length_3717_cov_6.402512"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="PLU28_09260"
     CDS             complement(<1..494)
                     /locus_tag="PLU28_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35285.1"
                     /translation="MPRFFSVSRKGFDWEPYVFLILFFAIFVPIASVMGISNFFRTIM
                     STSFDLLINTVFFIMAVAVITGALASVFSEFGVVSLLDKILSPLMMPLYRMPGAASLG
                     IVTTFLSDNPAILSLAKDNEFIRYFKKWQVPLLCNLGTSFGMGLIVSTIMLTYSNKMG
                     VNLFV"
     gene            complement(598..1401)
                     /locus_tag="PLU28_09265"
     CDS             complement(598..1401)
                     /locus_tag="PLU28_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG35286.1"
                     /translation="MSITADSISYTYGINTPFEKTAIKNVSIEISNGDLWLFVGHTGS
                     GKSTLINTFNGLILPQKGSVSVDGEVTGNKNTNMKKIRNKVGLIFQYPEAQFFLPTIY
                     EELMYGPKNFGMSLGKEELKFYMEMFSLPLGYLNKSPFNLSGGETRKVAIISVLCCNP
                     QYVVFDEPTVGLDYTSRTEVVKAIKRLNTMGKAIVLVTHWISDFIHLKPKVLVMNDGE
                     VLFKGSFDDFIRLDRPVLEQAGIRFDQKLELYRCALVHNRDIKDKISII"
     gene            complement(1392..2144)
                     /locus_tag="PLU28_09270"
     CDS             complement(1392..2144)
                     /locus_tag="PLU28_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG35287.1"
                     /translation="MFFGCENLNFEYADQKILKDLTLNFNKGEFVGLVGDNGSGKSTL
                     MKVLSGILLPQSGKVLFDDTVMSPKNKQYVGYIFQNPENQIVGVTVEEDVAFGLENIG
                     IQRESMIELISWALDVVGLSKYAKADPNTLSGGQKQRLAIAAIVAMKPEIILMDEPTT
                     MLDPIGRSEVYEVIKKLVSLKKTIIIASHHSDDLSLVNRIIGLKEGKIIFDGSRDSFY
                     SSGKLAVEVPFDIKLKKNFNKSFEQMVDHLCQ"
     gene            complement(2147..2896)
                     /locus_tag="PLU28_09275"
     CDS             complement(2147..2896)
                     /locus_tag="PLU28_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOG35288.1"
                     /translation="MDKKKKKKVEKTEEVQVMGPEELAHLSVKDVLGIFSSKKEEKPV
                     PEEKVEKFEHYYTAEPTSELHTKIIQIELKNGHVYNFTAPSGVYGKKRVDKATDLLVN
                     TAEISGKKLLDIGCGYGVIGITLKKENPFMQVYMSDINKRAVEFSKINAKDNNAQVTI
                     KWGNIFDPWEDEKFDVIISNPPIVAGKNIWMNIIKGSFIHLNPEGSLQLVAFHNKGGE
                     RISQFMKDTFGNMTELIKSGGIRVYKSVKEE"
     gene            complement(2911..3582)
                     /locus_tag="PLU28_09280"
     CDS             complement(2911..3582)
                     /locus_tag="PLU28_09280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35289.1"
                     /translation="MKKTFLLVFIMILSSFSIAGTFLSLGTYDGLGVGYADQISYLRV
                     SMKDMSFSSKVKFFNHYEAYLAVNFLFNKPVENNNIGAGLKIISENFSLDGGFWNSFF
                     EKQKVGEDEIVGQFGMKIGTDFRLNNLLAGVSLNYAMMNVTKNESGLNYYMQALPIEL
                     GFIRGFSGYIGYRLFSKDDFKTDFIINFSGKYSVIPGGMVFYKISSNNDINYSFSLKV
                     DGLRF"
     gene            complement(3603..3702)
                     /gene="ffs"
                     /locus_tag="PLU28_09285"
     ncRNA           complement(3603..3702)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLU28_09285"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
BASE COUNT         1147 a          756 c          603 g         1211 t
ORIGIN      
        1 acaaaaagat ttacacccat tttatttgaa taagtcagca ttatggtgct tactataagt
       61 cccattccaa aagatgttcc aagattgcaa agcagtggaa cctgccattt tttgaagtat
      121 cgtataaact cattatcctt tgccagcgaa aggatggcag gattatcgga aagaaaagtc
      181 gttactattc caagcgatgc tgctcctggc atgcggtata agggcatcat gagcggagag
      241 aggatcttgt ccagaagcga taccactccg aactcggaga agactgaggc aagtgctccg
      301 gttattacag ctactgccat tataaaaaaa acagtattta tcaacagatc aaaagatgtc
      361 gacatgatgg ttctgaaaaa attggatatg cccatgacag atgcaatagg aacaaaaatc
      421 gcaaaaaaca gaattaagaa gacgtatggt tcccagtcaa aaccttttct gctaacgcta
      481 aaaaaacgcg gcactttaat ccctcctgaa aaaacatatt caaaaatagt atattctact
      541 atgaaaaaaa tttcaataac aaaaaagcaa aaagaaaagg cttttaaaag ccttaatcta
      601 gattattgat attttgtctt ttatatctct gttatgaaca agagcacatc tataaagttc
      661 aagcttctgg tcgaacctta ttccggcctg ttcaagaact ggtctgtcca gtcggataaa
      721 atcatcgaat gatcccttga aaaggacttc accatcattc ataactagaa ctttaggttt
      781 aagatgtata aagtcactta tccaatgagt gacaagaaca atggctttac ccatagtgtt
      841 gagtcttttt attgccttta caacttctgt acgtgacgta taatcaagac ctacggtagg
      901 ttcatcaaat accacatatt gaggattaca acaaagaaca gatattatag ctactttccg
      961 agtctctcct ccagacagat tgaaaggact tttattgaga tagcctaatg gtagagaaaa
     1021 catctccata taaaacttaa gttcttcttt tccaagggac atcccaaaat ttttcggtcc
     1081 atacataagt tcctcgtata tggttggaag gaaaaactga gcttctggat actggaaaat
     1141 aagacctact ttatttcgta tctttttcat atttgtattt ttattgcctg ttacctcgcc
     1201 atcaacgctc acgctacctt tttgcggaag gataagtccg ttgaaagtat ttatgagtgt
     1261 acttttccct gaaccggtat gacctacaaa aagccataag tctccatttg atatttcaat
     1321 agaaacattt tttatggctg ttttttcaaa aggtgtattg attccgtaag tataacttat
     1381 actatctgct gttattgaca taaatgatcc accatctgct caaaactctt attgaagttt
     1441 tttttgagtt ttatatcaaa aggtacttcg actgctaact ttcctgagct ataaaaagaa
     1501 tccctgctac cgtcaaagat aattttgcct tctttaagcc ctattatccg gtttaccaag
     1561 gaaagatcat ctgaatgatg tgaagcaatt attatagttt ttttaagaga caccaatttt
     1621 ttaataacct catatacttc agaccttcct ataggatcaa gcatggttgt gggttcatcc
     1681 ataagaatta tttcgggctt cattgctact atcgctgcaa ttgcaagtct ttgcttttga
     1741 ccgcctgaaa gcgtattcgg atcagctttt gcatattttg ataatccaac gacatccaat
     1801 gcccatgaga taagctctat catactttct ctttgaatac caatattttc aagtccaaaa
     1861 gctacatctt cttcaactgt tactcccact atctgatttt cgggattctg aaatatgtag
     1921 cctacatact gtttgttttt tggagacatc acggtatcat caaaaagtac ctttccgctt
     1981 tgaggcagga gtattccact cagtactttc ataagagtag actttccgct tccgttgtcg
     2041 cctaccagac caacaaactc tcctttgtta aagttaagag ttaaatcttt aagaattttt
     2101 tgatctgcat attcaaaatt taaattttcg catccaaaaa acatgattat tcttccttta
     2161 cggatttata tactcgtatt ccgcccgatt ttataagttc agtcatattt ccgaaggtgt
     2221 ccttcataaa ctgcgaaatc ctttctccac ctttgttatg aaatgctaca agctggaggg
     2281 aaccctcagg gttgagatgt ataaatgaac cttttattat attcatccaa atgttttttc
     2341 cggcaactat aggaggattt gaaattatta cgtcaaactt ttcgtcttcc cacggatcaa
     2401 aaatgttgcc ccattttatt gtaacctgtg cattgttgtc tttagcattt atttttgaaa
     2461 attcaactgc tcttttgttt atgtcactca tataaacctg catgaaagga ttttcctttt
     2521 ttaaagtaat acctattacc ccatagccac aaccgatatc taaaagtttt ttaccgctta
     2581 tctcagcggt attgacaaga aggtctgtag ccttatcgac tcttttttta ccgtaaactc
     2641 cactgggggc tgtaaaatta taaacatggc catttttcag ttctatctgt attatcttgg
     2701 tgtggagttc cgaagtgggt tctgcagtat agtagtgttc aaacttctca actttttcct
     2761 ctggaacggg tttttcttct tttttagaag aaaaaatacc gagtacatcc ttaaccgaga
     2821 gatgagcgag ttcttccggt cccataacct gaacttcttc agttttttca actttctttt
     2881 tctttttttt atccataaga tcactccgta ttaaaatctt aagccgtcaa ctttaagtga
     2941 gaaactatag tttatgtcgt tattgctact tattttgtaa aaaaccattc caccgggaat
     3001 aaccgagtac tttcctgaaa aattgattat aaagtcagtt ttaaaatcgt cctttgaaaa
     3061 caatctgtat cctatatatc cagagaatcc tcttataaag cccaactcta taggaagagc
     3121 ctgcatatag tagttcaaac ctgattcgtt tttagttaca ttcatcattg cataattaag
     3181 acttacacct gcgagtagat tattcagcct gaaatctgtt cctatcttca ttccaaattg
     3241 accgacaatc tcatcttctc ccactttctg tttttcgaaa aaggagttcc agaagccacc
     3301 atcaagagaa aaattttcgg atataatctt aaggccggcg ccgatgttat tattttcaac
     3361 aggtttatta aataaaaaat taactgccag gtaagcttca taatgattaa aaaactttac
     3421 tttagaggaa aatgacatgt ctttcatgga aactctaaga taactgattt gatcagcata
     3481 cccaacacct aagccatcat aggtgcctaa agacaaaaag gtgcctgcta tgctaaaaga
     3541 gctcagaatc attataaaaa ctaaaagaaa agtttttttc atgactatcc tccaaaaata
     3601 aaggtggccg agtatcccca cggcacacaa agaattctgc ttatggctgc tcccttccgg
     3661 gcctgaccag attcacagct ccccgttgcg tgggacccgg ccacgcgtag gtccact
//
LOCUS       DAOSHR010000195         3713 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_22916_length_3713_cov_4.974030, whole genome shotgun sequence.
ACCESSION   DAOSHR010000195 DAOSHR010000000
VERSION     DAOSHR010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 3713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3713
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22916_length_3713_cov_4.974030"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..407)
                     /locus_tag="PLU28_09290"
     CDS             complement(57..407)
                     /locus_tag="PLU28_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HOG35290.1"
                     /translation="MGDFDEIYDIILRFRKEREWEKFHSPKNLAVSISLEAAELLEIF
                     QWDNSGAQSSETAQKRIGEVKDELADILVYSMFMAKDLNIDIKNAIKEKMEKNALKYP
                     VEKARGSSKKYNQL"
     gene            complement(400..1119)
                     /locus_tag="PLU28_09295"
     CDS             complement(400..1119)
                     /locus_tag="PLU28_09295"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOG35291.1"
                     /translation="MEKEFYRQVLGGINAGNILDVGCREGYFIGIMLESFQSVASVSA
                     IDISDKGYLKEKDFFEKNHVDFKVMDGANMEFKESTFDTVSISNSIHHLTDVSAVLAE
                     MKRVMKKDGLFILNEMICDTSDVSRKKDVSVHHWFARTDSIMGTFHRMTYTSSELFGF
                     VNSLGFGKIHIRYFLDDEYSDVQNNEKLIASIEKRLLQKEWDDDVVTEGRELIKMLKT
                     GCFKQAPVMTIIARGKDEKNG"
     gene            1451..2593
                     /locus_tag="PLU28_09300"
     CDS             1451..2593
                     /locus_tag="PLU28_09300"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOG35292.1"
                     /translation="MSYTLSANEENIVRLVRTRGLISRADLSRETGLSKPIVSNIVSK
                     LINEGAIVEKEIGMSSEKGGKKPILLSFASTYKYIVGVDVGGNKLIAILTDLDGNIIN
                     KVRKVTKGIKSKNALIEIIFQGIEEVITVDKAMILGVGIGVPGTTDIKTGFVYYMPAF
                     ELSDIPLGEIVSSHFGIPVMISNDVTVNALGELSKGSARNIKDLCLIAIGTGTGAGLI
                     LNGSIYSGSHNMAGEIGYMITDWNRENKSRNRTFGNYESWFSGNGLEIKVKEKLGLNL
                     TTKEIFDNLDKSRDIKEIFLQGCEHLALGIANIITLLDPQMIIITGGVGYNQYDMILR
                     YLMPFLEVIVPPEILRNISFSRGELGDLGVSVGGVSLIHDNYFLKM"
     gene            2643..>3713
                     /locus_tag="PLU28_09305"
     CDS             2643..>3713
                     /locus_tag="PLU28_09305"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOG35293.1"
                     /translation="MKRVTLFLIFVCVLFTLSVSAKTTIVYWQYFYQTKVDTINQLIK
                     EFEKLNPDIEVKQVTFPYENYNQKVAASIPAGIGPDVINLFYGWLPMYVNSGYLQELP
                     KNEFNEKYFKDNFFPFVEESVKFNNKYYSVPTAVRTLALFWNKKLFKQAGLDPEKPPK
                     TLTEAVEMAKILSKYDSKGNLLQAGFTMQPDGQHHHWIREVLVRQFGGTPYSTDNKKV
                     LYDKTGAAGEVLKWYTDRITKDKIGYPGFLNDDVTAFKSQKAAMTIDGSFRIGTLNTV
                     KDLEYGIAELPEHNGIKSNFASFWTHAITTNAQGERLTASIKFLKFITSKEAMELWLK
                     NVGELPANPEIANKYSEDKLYGP"
BASE COUNT         1183 a          713 c          683 g         1134 t
ORIGIN      
        1 ggcacatttt ttgtactttt ttcattttac cccttgactt ttaatagtta gtctctttat
       61 agttgattat atttttttga acttccacgt gccttttcaa cgggatactt taatgcattt
      121 ttttccattt tttcttttat agcgtttttt atatctatgt tcaggtcttt tgccataaac
      181 atactgtata caagaatatc ggcaagctcg tcctttactt ctcctattct tttttgagct
      241 gtttcagagc tctgagcacc gctgttgtcc cattgaaata tctcaagcag ttctgccgcc
      301 tcaagtgaaa ttgataccgc aagattttta ggactgtgaa acttctccca ctctctttct
      361 tttcgaaacc tcaaaatgat gtcgtaaatt tcatcaaaat cacccatttt tttcatcctt
      421 tccacgagca attatagtca ttaccggagc ctgcttaaag caacctgtct taagcatttt
      481 aattagctct ctcccttcgg ttacaacatc atcatcccat tctttctgta aaagcctttt
      541 ttcaatgctc gcaatcagtt tttcattgtt ttgaacatca gaatactcat catccagaaa
      601 gtacctgatg tgtatttttc caaatccgag actattaaca aacccaaaaa gctccgaaga
      661 ggtataggtc attctgtgga aagtacccat aatactgtcg gttcttgcga accagtgatg
      721 aacgctaaca tctttctttc ttgatacgtc gctggtatca catatcatct cattaagaat
      781 gaaaagacca tcttttttca ttacacgttt catctcagct aaaacggctg atacatctgt
      841 aagatgatga attgagtttg aaatactgac agtatcaaaa gtactttctt taaactccat
      901 attcgcgccg tccatgactt tgaaatcaac atgatttttt tcaaaaaaat ccttctcttt
      961 aagatatcct ttgtctgata tatctatggc tgacacagat gcaacactct gaaaagattc
     1021 aagcattata cctataaaat agccctccct gcagccgaca tcaagtatgt tgccggcgtt
     1081 aataccccca agcacctgtc tgtaaaattc tttttccatt ttttcctcct gtcctccgaa
     1141 aggttaaaaa acttctgttt aaggtatata aactgtatcc tgtatgtaaa tattataaca
     1201 aaaaatatgc tttttattgt taaaaagact tcttattatt attagtactt tatacaaact
     1261 atatgatcct ttggaataca ataacttaaa aagttcgatg ttcttattta aaaaaatact
     1321 ttttatatat atgaatgact ttaacaatta gtaatcacac atgaaggccg aagaaaaggt
     1381 tttgatttat atgtaacata ttttaaaatt tgtttgttag taactttaac taactaacgg
     1441 aggtttttcg atgtcatata ctttatctgc taatgaagaa aatattgtaa ggctggtaag
     1501 aacaagaggt ttaatatcac gcgcggacct ttcaagggag acgggacttt caaaaccaat
     1561 tgtttcaaat atagtttcaa aattaataaa cgaaggcgct attgttgaaa aagaaatagg
     1621 catgagttcc gaaaaaggag gtaaaaagcc gatacttctt tcattcgcaa gcacctacaa
     1681 gtacatcgta ggagtagatg tgggaggaaa taagcttatc gcgattctta ccgatcttga
     1741 tggaaatatc ataaataaag tcaggaaagt tactaaaggc ataaaaagca aaaatgcctt
     1801 gattgaaata atatttcagg gaatagaaga agtgataact gttgataaag ccatgattct
     1861 gggagtaggc atcggagttc cgggaacaac cgatataaag actggttttg tctattatat
     1921 gcccgcattt gagctcagcg atattccatt gggagagata gtgagcagcc atttcggaat
     1981 acctgtcatg atatccaatg atgttacagt caatgctttg ggagaacttt caaaaggttc
     2041 ggcccgaaac ataaaagatc tttgtcttat agctataggc actggcaccg gggcaggact
     2101 catcctcaat ggttccatct attccggaag ccataatatg gctggagaaa tagggtatat
     2161 gataacggat tggaacaggg aaaacaaatc cagaaaccga acttttggta actacgaaag
     2221 ctggttttcc ggcaatgggc ttgaaattaa agttaaggaa aaattaggct taaatcttac
     2281 aaccaaagag atttttgata atcttgacaa aagcagggat ataaaagaaa ttttccttca
     2341 gggttgtgaa cacctggctt taggcattgc aaacataatc actctgcttg atccccagat
     2401 gattataatt accggcgggg tgggatacaa ccagtacgac atgattctca ggtatctcat
     2461 gccttttttg gaagtaatcg tgccgccgga aattctcagg aatatctctt tcagcagggg
     2521 agaactcggt gatcttggag tctctgtcgg aggagtgagc ctaatacacg acaattattt
     2581 tctgaaaatg tgaatgtttt ttatacaata agggtatatt ttaaaactca ggaggtgttg
     2641 gtatgaaaag ggttacatta tttttgatct ttgtttgtgt tttatttact ctaagtgtgt
     2701 ctgcaaaaac gactatagtt tactggcagt acttctatca gacaaaggtg gatactataa
     2761 accagttgat aaaagagttc gaaaaactga atccggatat agaagtaaag caggttacct
     2821 ttccctatga aaattacaat cagaaggtag ccgcttcaat acctgcagga atagggccgg
     2881 atgtaataaa cttgttctac ggttggctcc caatgtacgt taactcagga tatctccagg
     2941 agcttccgaa aaatgagttt aatgaaaagt actttaaaga taatttcttt ccttttgtcg
     3001 aagaaagtgt caaatttaac aataaatact actccgtacc cactgccgta agaacccttg
     3061 cacttttttg gaacaaaaaa ctgtttaaac aggccggtct tgatccggaa aaaccgccca
     3121 agacccttac agaagcggta gaaatggcga aaatcctttc aaaatacgac tcaaagggaa
     3181 atcttctcca ggccggattc accatgcagc cggacggaca gcatcaccat tggataagag
     3241 aggttcttgt acgacagttt ggtggaactc cgtactcgac agacaacaaa aaagtcctct
     3301 acgataaaac gggagcagcc ggagaagttt taaaatggta tacagataga ataaccaaag
     3361 ataaaatcgg ttatccgggc tttttgaacg atgacgtgac tgctttcaag tcacagaaag
     3421 ctgctatgac tatcgacggt tccttcagaa taggaactct taatacggta aaagaccttg
     3481 aatatggcat tgccgagcta ccagaacaca atggaataaa atccaatttt gcatccttct
     3541 ggactcatgc cattaccacc aatgcccagg gagaaagact gacagcctca ataaaattcc
     3601 tcaagttcat aacctccaaa gaggctatgg agctttggct caaaaatgtc ggagaactgc
     3661 cggccaatcc ggaaattgca aataagtact ctgaagataa actttacgga ccg
//
LOCUS       DAOSHR010000196         3694 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_23081_length_3694_cov_5.375927, whole genome shotgun sequence.
ACCESSION   DAOSHR010000196 DAOSHR010000000
VERSION     DAOSHR010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 3694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3694
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23081_length_3694_cov_5.375927"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..725
                     /locus_tag="PLU28_09310"
     CDS             <1..725
                     /locus_tag="PLU28_09310"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG35294.1"
                     /translation="YTPYPVTQMNIKERFSPPSPKHFFGTDNFGRDILSRVMIGSQTA
                     FFVGALAVFIGLFFGVLIGALSGYIGGIFDEITMRIMDAMLAFPGILFALMFVAIFGV
                     GIKNTITAIGIMSIPSFARVTRSSYLQHKEFEYVKSAKAKGASPLRIIFFHILPNVTS
                     PIIVSSTLGFARAVLSEAGLSYLGLGVQPPYPSWGRMLNESQVFISIAPWYAIAPGVF
                     ITLLVLGFNFLGDGLQDLESLK"
     gene            734..1720
                     /locus_tag="PLU28_09315"
     CDS             734..1720
                     /locus_tag="PLU28_09315"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG35295.1"
                     /translation="MNEKLFEIKDLSISFKKKDEQFFAVKSVDFDIHKGEIVGLVGES
                     GCGKTLTGLSIIGLTPGDAVISGQINFKGMNLLSKNKEERRSTRGKSISMIFQEPMTS
                     LNPLMKVGDQIRETLLINMPIDKLQAKQKVYEIMKSVGFADVCNLYKKYPHELSGGMR
                     QRVVICIAIACNPDLIIADEPTTALDVTVQAQILELLKKINLETNSSILFISHDFGVI
                     KEMCSQVIVMYAGYIVEKSCIERIFSNPIHPYTQALLKCIPAPEKKGNPLFSIKGKVP
                     SIYDNSEGCPFFNRCSYALERCRKELPELREYEPEHLTRCFYAEEKNGKTDS"
     gene            1701..2672
                     /locus_tag="PLU28_09320"
     CDS             1701..2672
                     /locus_tag="PLU28_09320"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HOG35296.1"
                     /translation="MEKPILEVRNLKKYFPLRRNNIFSQIKYLKAVNDVSFSVQKGEI
                     FGIVGESGCGKSTLGQTLMHLYKPTSGRIIFNGQDISFVDGRILKELRKKIQIIFQDP
                     YGSLNPRKKIGWILEEPLIIHGMKDKKKRMDKVSELMELAGFNTSYLNRYPSELSGGQ
                     RQRICIISSLILEPELIIADEPVSALDVSVQAQILNLMTDLQKNYNLTYIFISHNLNV
                     VHYISTRIAIMYLGEFVEYGQAERIYTAPLHPYTTALFSAVPTLDQNKKERIIIEGEL
                     PDPVNPPVGCPFNTRCRHAFERCFKEKPEFTKIDDNHYVKCHLYGKE"
     gene            2674..3477
                     /locus_tag="PLU28_09325"
     CDS             2674..3477
                     /locus_tag="PLU28_09325"
                     /inference="COORDINATES: protein motif:HMM:TIGR03729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOG35297.1"
                     /translation="MKAAVISDIHIDINHDEKEPVIDSLKKVLEQDMPEILIIAGDIS
                     NDYRKTLEFLKQTDRTIPTLFVPGNHDLWSIENQQHDTWKIYDALGTYPHNLCTQNYI
                     INDEWAVCGECGWYDYSFADREFSTELIQEGSYMQRKWKDKDYIKWGKNDAEVFDIFY
                     DKLEKKISEVKDRKIIMVTHMLTHKEFTVKDHPMWKFFNAFLGSSRYGELIKKYDVKY
                     SIMGHVHYRKELEENGTRYICACLNYTNEWEGSCSPYYQIKQTIKYINI"
BASE COUNT         1269 a          693 c          730 g         1002 t
ORIGIN      
        1 tctatactcc gtatccagtt acacagatga acattaaaga aagattcagt ccgccttctc
       61 ctaaacattt ctttggaact gacaactttg ggcgtgatat actatcaaga gttatgatcg
      121 gatcacagac cgcttttttt gtgggagcac tcgccgtttt catcggattg ttttttggag
      181 tgcttatagg agcacttagt ggatatatag gcgggatttt tgatgaaata acaatgagaa
      241 taatggatgc catgcttgca tttcccggaa tactttttgc actaatgttt gtggcaattt
      301 tcggggtagg tatcaaaaat accataacag caataggaat aatgtctatt ccatcttttg
      361 ccagagttac aagaagcagt tacctgcagc ataaagagtt tgaatatgta aaaagtgcta
      421 aggcaaaggg agcctctcct cttaggatca tattttttca tattcttccg aacgtgacct
      481 ctcccattat agtgtcatct accctcggat ttgcacgggc agttctttct gaggccggat
      541 taagctatct cggtctggga gttcagcctc cgtatcccag ctggggaaga atgcttaatg
      601 aatcgcaggt atttataagc atagctccat ggtatgcaat agcccccgga gtatttataa
      661 cccttcttgt cctgggattt aattttcttg gagacggact tcaggatcta gaaagcctaa
      721 aataaggtgt catatgaatg aaaaactatt tgagataaaa gatttaagca taagctttaa
      781 gaaaaaggat gaacagtttt ttgctgtaaa gtcggttgac tttgacatac ataaaggcga
      841 aattgtagga ctggtgggtg aaagcggttg cgggaaaacc ctcacaggac tttcgataat
      901 aggacttacg ccaggtgatg ctgtcataag cggacagatc aacttcaaag gtatgaacct
      961 tctgtcaaaa aacaaagaag aacgaaggag tacccgcggt aaaagtatat ctatgatctt
     1021 ccaggagccg atgacctctt tgaaccctct tatgaaagtt ggagatcaga taagggaaac
     1081 ccttcttata aatatgccga tagataagtt acaggctaag caaaaagtat acgaaataat
     1141 gaaaagtgta ggctttgcag atgtatgtaa cctatataaa aaatatccgc atgaactttc
     1201 cggaggtatg cgccaaagag tagtgatatg tatagcaata gcctgcaatc cggatcttat
     1261 aatcgcagat gaaccaacga cagcgcttga tgttaccgtt caggcacaga tacttgagct
     1321 actgaaaaag atcaacctgg aaaccaacag ttcaatactc ttcatatcac atgatttcgg
     1381 agtaataaaa gaaatgtgca gtcaggttat agtgatgtat gccggatata tagtagaaaa
     1441 atcctgtata gaaagaatat tttcaaatcc cattcatcca tatacccagg ctcttcttaa
     1501 atgtattccg gctcccgaaa aaaaaggaaa tccacttttc agcataaaag gcaaagtacc
     1561 ctctatttat gataattctg aaggatgccc gtttttcaac cgttgttctt atgctcttga
     1621 aagatgcagg aaagagcttc ccgaactaag agagtatgaa cctgaacatc ttacaagatg
     1681 cttttacgcg gaggaaaaaa atggaaaaac cgattcttga agtcagaaat ctcaaaaaat
     1741 actttccatt gagaagaaac aacatatttt cccagataaa gtatcttaaa gccgttaatg
     1801 atgtaagctt ttcagtacag aagggagaaa tattcggcat agtcggagag agtggctgtg
     1861 gaaagtcaac tctcggtcag acacttatgc atctttacaa acccacatcc ggcagaataa
     1921 tatttaacgg tcaggatata tcctttgttg acggcagaat actcaaagaa ctgagaaaaa
     1981 aaatacaaat aatttttcaa gatccttacg gctcactcaa cccacggaaa aaaataggct
     2041 ggattcttga agaaccgctt ataatacacg ggatgaaaga caaaaaaaag agaatggaca
     2101 aagtttctga acttatggaa cttgcaggct ttaacacctc ttatcttaac agatatccaa
     2161 gtgaactttc cggaggtcag agacaacgga tatgtataat aagctcgctt atacttgaac
     2221 ctgagctgat aattgccgat gaacccgttt cagctcttga cgtatcggtt caggcgcaga
     2281 ttctcaatct gatgacagac ctacagaaaa attacaacct tacatatatt ttcatctcac
     2341 ataaccttaa cgtggttcac tacataagta ccagaatagc cataatgtac ctcggagagt
     2401 ttgttgaata cggacaggca gaaagaatat atactgcccc gcttcatccg tatacaaccg
     2461 ctcttttttc ggcagttccc acattagatc aaaataaaaa agaaagaata attattgaag
     2521 gagagcttcc tgatccagtt aacccacctg tcggatgccc attcaatacc cgttgccggc
     2581 atgcttttga aagatgtttc aaagaaaaac cggaatttac aaaaattgat gacaaccatt
     2641 atgtaaaatg ccatctctat gggaaggaat aatatgaaag ccgctgtcat atctgatata
     2701 catattgaca taaatcatga cgaaaaagaa ccggttattg actcgctcaa aaaagttctt
     2761 gaacaggata tgccggaaat acttataatt gcgggagata tctccaacga ttaccggaaa
     2821 acccttgagt ttttaaaaca aacagaccga actattccaa ccctttttgt tcctggcaac
     2881 catgatctgt ggagtataga aaatcaacag catgacacat ggaagatata cgatgcttta
     2941 ggaacttatc cgcataacct gtgtacacag aactatataa taaacgacga atgggccgtt
     3001 tgcggagaat gtggatggta cgactactca tttgcagaca gagagttttc tacagagctt
     3061 atccaagaag gcagctatat gcaaagaaaa tggaaagata aagattacat aaaatggggg
     3121 aaaaatgatg ctgaagtctt tgatatattt tacgataagc ttgaaaaaaa aataagtgaa
     3181 gtaaaagaca gaaagataat aatggttaca catatgctta cacataaaga atttacagtt
     3241 aaagaccacc ctatgtggaa gtttttcaac gcttttcttg ggagcagccg ttacggagag
     3301 cttataaaaa aatatgatgt gaagtactct attatggggc atgtacatta ccgtaaagag
     3361 cttgaagaaa atggaaccag gtatatctgt gcctgcctga actacacaaa cgaatgggaa
     3421 ggctcctgtt ccccctatta ccagataaaa cagaccataa aatatataaa catataaaaa
     3481 atactcatca gaactgtgtg aaacaggtgt gtatgaaata gatatttttg tatgtaaggc
     3541 aggttattcc aaccatacga catacggtct tgagatagaa aagaataaag tgaacctatt
     3601 tcgggtacct attcccagtg aactataaaa tcaatactaa aattaaaggc ggacaactac
     3661 tgtccgcctt ttcactactc ttttatccaa tcat
//
LOCUS       DAOSHR010000197         3680 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_23193_length_3680_cov_5.878069, whole genome shotgun sequence.
ACCESSION   DAOSHR010000197 DAOSHR010000000
VERSION     DAOSHR010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 3680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3680
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23193_length_3680_cov_5.878069"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="PLU28_09330"
     CDS             <1..387
                     /locus_tag="PLU28_09330"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HOG35298.1"
                     /translation="EEFDIMKQHVLFGENILKDMDGKIQNMTGFSFMKTAIDIAGGHH
                     EKWDGSGYPRKLSGHDIPLAGRIVALADVFDALTSARPYKKAFDFDLSVRMIIEQSGS
                     HFDPDVVHAFTTGLDSIRRIYDRYRE"
     gene            690..1202
                     /locus_tag="PLU28_09335"
     CDS             690..1202
                     /locus_tag="PLU28_09335"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HOG35299.1"
                     /translation="MELKEYVRDILDFPQKGIIFKDITPILKDPEAFGYTIKILQELL
                     KDWDFDFIVAPEARGFLFASPLAYTMKKGLIPVRKPGKLPYKTVNISYNLEYGSAELQ
                     MHVDALKKGDRVVIVDDVLATGGTLDAIRRMVNNVGADVEGVLCMAELGFLKGRERLK
                     DLKVASILKY"
     gene            1217..2584
                     /locus_tag="PLU28_09340"
     CDS             1217..2584
                     /locus_tag="PLU28_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HOG35300.1"
                     /translation="MEGISFDFTNLFSPVLKGGLTKKDINSYKEKASEAVENILNSNP
                     GFMGVPFESKWLESVRELKEWILSFESLVVLGIGGSALGNIALQSALNPADYNNQKNR
                     KTPRIYIVDNVDPDLLSSVLDQIDIKTTLFNVISKSGTTAEAMANYMVARGLLETNGL
                     KVQDHILFTTDPQNGILRKMSRDENIRAIEIPPQVGGRFSVLTPVGLVSAMAGGIDIQ
                     ELFLGARDMCEKCKNKDIWKNPAALNAVIHYLYYLKGYDLSVMMVYSNRLYLLADWYR
                     QIWAESIGKKLNLKKETVNVGQTPVKALGATDQHSQVQLYNEGPKDKIITLIKLENFE
                     REVSIPKIHEDIAELKYLGGRKLSELLNTELAGTEYALLEHERPNMRVTFPRISAYNI
                     GEFIFMYEFSTAVMGGLLQVDPYDQPGVELGKKVTYALMGREGYEDLAAEVMKKIQVK
                     NRVIM"
     gene            2581..3186
                     /gene="coaE"
                     /locus_tag="PLU28_09345"
     CDS             2581..3186
                     /gene="coaE"
                     /locus_tag="PLU28_09345"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOG35301.1"
                     /translation="MIIGITGLAGSGKSYVSKIMKEKAESALMKKVLVIDADRVGHDV
                     LSLFFIRQELVKAFGNDILNENGEIDRKALGRKVFSSRAKLAVLNAVVHPVIRQEVEK
                     MLKEKEKSYDIIIIDAALLDQIKLAEMCNIIVLVECSEDKRVDRLILNRSIPIEKAHA
                     IVQSQKELNLGRSDFKIINEGNFESTCRQVEDFLSQLTSVE"
     gene            3262..>3680
                     /locus_tag="PLU28_09350"
     CDS             3262..>3680
                     /locus_tag="PLU28_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOG35302.1"
                     /translation="MKKLLIVLLVALFATFAFADKVTVEFWHAMSGDLGKVLQNLVDQ
                     FNKENPTIEVKSVYVGNYNALNQKLLATITAYNQGTKSGLPAMAQSYGNWTAKYLYSD
                     VVKALNSYITKDPEMKKAWDTQIYPVFKDIVSWGDMI"
BASE COUNT         1220 a          576 c          848 g         1036 t
ORIGIN      
        1 gaagagtttg atataatgaa gcagcatgtg cttttcggag aaaatattct taaggatatg
       61 gacggcaaga tacagaacat gaccggtttt tcttttatga agactgctat agatattgcc
      121 ggggggcatc atgagaaatg ggacgggagt gggtatccgc gaaagctaag cgggcatgat
      181 attcctcttg ccggaagaat agtagctttg gcagatgtgt ttgatgctct gaccagtgct
      241 cgcccatata aaaaagcttt tgattttgat ctttcggtaa ggatgataat agagcagtcc
      301 ggtagtcact ttgacccgga tgtggttcat gcgtttacta ccggcttgga tagtataaga
      361 agaatctatg accggtacag ggaataaaaa ttttctagta ttgacatgtc cggagaaagg
      421 tactataatt ttattataaa aaaattctgt cagaataatt attgctcaaa aaagggggcg
      481 gtataaatgg cggaaaaaaa agaagtgaaa aaagaagtta aaaaggaacc agaaaagaaa
      541 gaaactccga aaaaagaaaa gaaatagttt ttaagagccg ccattttatg gcggctttat
      601 atattttatt aaaaaaaagt tacaatataa tctaaaaaaa tatggtacaa tatacgatgt
      661 cattatttat tctttagagg aggaaaagta tggaattaaa ggagtatgta agagatatac
      721 ttgattttcc acagaaaggg attattttta aggatattac gccaatacta aaagatccgg
      781 aggcattcgg atacaccata aaaatacttc aggaactgtt aaaggactgg gattttgatt
      841 tcatagtcgc accggaagcc aggggttttt tatttgcctc tcctttggca tatactatga
      901 aaaaaggtct gataccggta aggaaaccag gaaagcttcc ttataaaaca gttaatatat
      961 cttataatct tgagtacgga agtgccgagc ttcagatgca tgttgacgca cttaaaaagg
     1021 gtgacagggt ggtaatagtt gacgatgttc tggcaaccgg gggaacactt gatgcgataa
     1081 gaagaatggt caacaatgta ggcgcagatg ttgaaggtgt actctgcatg gctgaactcg
     1141 gtttcttaaa aggtagggaa cgtcttaaag atcttaaggt tgcttcaatc cttaaatatt
     1201 aagaaggagg cggagtatgg aagggatctc ttttgatttt accaatctgt tttcaccagt
     1261 tttaaaaggc ggacttacca aaaaggatat aaacagctac aaagaaaaag cttctgaagc
     1321 ggttgagaat atacttaaca gtaatccggg atttatggga gtacctttcg aatcaaaatg
     1381 gcttgagtca gtcagagagc ttaaagaatg gatactttct tttgaaagtc ttgtagtttt
     1441 aggaataggt ggatctgctc ttggtaatat tgctttgcaa agcgccctta atcctgcaga
     1501 ctataataac cagaaaaaca gaaaaacccc taggatatat atagtggata atgttgatcc
     1561 ggacctcctt tcaagtgttt tggatcagat agatattaaa acaactcttt ttaacgttat
     1621 ttcaaaatcc ggaaccacag ccgaggctat ggcaaactat atggtggcca ggggactgct
     1681 tgagaccaat ggtcttaaag ttcaggatca tatattattt acaaccgatc ctcaaaacgg
     1741 catacttcgg aagatgtcac gtgatgaaaa tataagagct attgagattc ctccgcaggt
     1801 aggtggaagg ttcagcgtgc tcactcctgt cggacttgtt tcagctatgg caggaggaat
     1861 tgatatacaa gagctttttc taggtgcacg cgatatgtgt gaaaaatgta aaaataaaga
     1921 tatatggaag aatccggcgg ctcttaatgc ggtaatacat tatctttact atttaaaagg
     1981 atatgactta tccgtgatga tggtttactc caacagactg tatcttcttg ccgattggta
     2041 caggcagata tgggctgaaa gtataggaaa gaagcttaat ctcaaaaaag aaacggtcaa
     2101 tgtggggcag actccggtaa aagctcttgg tgctactgac cagcattcac aggttcagct
     2161 ttacaacgaa ggaccaaaag ataagataat aactctcata aagcttgaga attttgaaag
     2221 ggaagtttcg attcccaaaa tacatgaaga tattgccgaa ctgaagtatc ttggcggaag
     2281 aaagctttcc gagcttctta atacagagct tgcaggtacg gagtatgcac ttttggaaca
     2341 cgaaagaccc aatatgagag ttacatttcc cagaatcagt gcttataaca tcggggagtt
     2401 cattttcatg tatgagttct caactgctgt gatgggaggt cttttgcagg ttgatcctta
     2461 tgaccagccc ggtgtggagc ttgggaaaaa ggttacgtat gctcttatgg gacgtgaagg
     2521 gtatgaggat cttgccgcag aggttatgaa aaaaatacag gtaaaaaata gggtgattat
     2581 gtgataattg gaataacagg tttagcaggc agcggaaaat cttatgtttc aaaaattatg
     2641 aaagaaaaag cagagtccgc gcttatgaaa aaggttttag tcattgatgc agacagggtc
     2701 ggacacgatg tcctttccct gttttttatc cgacaggaac ttgtaaaagc ttttggaaac
     2761 gatattttaa atgaaaacgg agagatagac agaaaagctc tgggcagaaa agtattttca
     2821 agccgtgcca agcttgcagt tttaaatgct gttgtgcatc ctgttataag acaggaagta
     2881 gagaaaatgt taaaagaaaa agaaaaaagt tatgatataa ttataatcga tgcagcattg
     2941 ttagatcaga taaaactagc tgaaatgtgt aacataatag ttcttgtgga gtgttccgaa
     3001 gataagcgtg tggacaggct gattctgaat aggagtatcc ctatagaaaa agcgcatgct
     3061 attgtgcagt ctcagaaaga actcaatctg ggcagatcag atttcaaaat tataaatgaa
     3121 ggaaattttg aaagcacttg caggcaggtt gaagattttc taagtcagct tacgtcggtg
     3181 gagtaagggg tttttatttt ctggataaat agaaataact taaaaaagtt atccaattct
     3241 tttattacgg aggtgctaaa ggtgaagaaa cttcttattg ttctgcttgt tgctcttttt
     3301 gctacatttg catttgcaga caaggtaacg gttgaattct ggcatgctat gagcggtgat
     3361 ctcggaaagg ttcttcagaa tcttgtagac cagttcaaca aggaaaatcc taccattgaa
     3421 gttaagtcag tttatgtagg caactacaat gctctgaacc agaaacttct tgccactata
     3481 acagcataca accagggaac caagtccggt cttcctgcaa tggcacagtc ttatggaaac
     3541 tggactgcca agtatctata ttcggatgtt gtaaaagctc ttaacagcta cataaccaaa
     3601 gacccggaaa tgaagaaagc atgggatact cagatttatc ctgttttcaa agacatcgtt
     3661 tcatggggag atatgatata
//
LOCUS       DAOSHR010000198         3670 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_23291_length_3670_cov_4.804979, whole genome shotgun sequence.
ACCESSION   DAOSHR010000198 DAOSHR010000000
VERSION     DAOSHR010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 3670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3670
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23291_length_3670_cov_4.804979"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="PLU28_09355"
     CDS             complement(<1..260)
                     /locus_tag="PLU28_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35303.1"
                     /translation="MYNKKVIKNKYVVLDSKLDINSKKIEEEILSAEDNQQNLLVDQQ
                     VKDRLVETEEKAKEIIFQANKRSEEIIKKANQQAQAIVEEGK"
     gene            complement(253..1263)
                     /gene="fliG"
                     /locus_tag="PLU28_09360"
     CDS             complement(253..1263)
                     /gene="fliG"
                     /locus_tag="PLU28_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HOG35304.1"
                     /translation="MAKNEDITGVKKAAVLLVLMGSDRAATILKKLPDSDVEQLTLEI
                     ANLGKIEESLKNSVSSEFFEFAKIKEFINKGGIDYARKLLEDAFGPEKAIEIIEKLVS
                     SLQVRPFDFLRRIDVAQMVNVLQNEHPQTIALVLCYLSPTIASQVITNLNESLQVEVV
                     KRISIMNSATPDVVKDVETKMKDRLSSFATQTFSQVGGIETAAEIMNNIDRVVSKNIF
                     DRLSERDPKLSEEIRKRMFVFEDIVKLDDRSIQRIIREIDNKDLTIALKGASEEVKLT
                     IFKNMSKRSSQIVQEELDYMGPVRVRDVDEAQQKIVATIRRLEESGEIIIGSGGGEEL
                     IV"
     gene            complement(1274..2824)
                     /gene="fliF"
                     /locus_tag="PLU28_09365"
     CDS             complement(1274..2824)
                     /gene="fliF"
                     /locus_tag="PLU28_09365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HOG35305.1"
                     /translation="MEEFLKKILGWWKNLEKKKKITYTIYALSVIIVMIVLFVVISLP
                     HYTLLVSGLNETQAGQVIAKLDEQGVKYKAGPNGSIYVSNSQKYELRMKLASEGVLGN
                     NSRGYELLQNQGFGATSFDKQVNYQIALEGELERSISAITGVSFARVHLVIPPKTYYT
                     VGDNAIPKASVMISLATGAQISQEQVKGIVELISGAVQGLEPKNVRVVDNYSRDLSSS
                     IYNDDIGSANTKFQMKQMIESYYTKKIQQTMQTVFGFGNVVVMSDVELNWQKLEQEER
                     TVQPVNKSSGIILSQQQESEQTINSSNNSGAPGTTSNIPPYDYQTESSDQNTGYSKSK
                     VITNYDINEIYKKTIDDRTGEIAKKSFTIYIDFLKSGVTETEEIIKNIKSSITTATGS
                     GQDNISVLSLTFNREMDNEKSKIEAENLRRRKLITYVILIMVAIVMLLFLLYVALLYM
                     RRKKTYTLIEERKKKLEERVKEVVGEVKEEPMELSPSQEMQKRLEAIVDSRPNDVVEI
                     IKIWINTQ"
     gene            complement(2842..>3670)
                     /locus_tag="PLU28_09370"
     CDS             complement(2842..>3670)
                     /locus_tag="PLU28_09370"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HOG35306.1"
                     /translation="EFLKAVWSQERSMIGGQIDVESDFYTHAISSSKALGLMQIKYQT
                     ASDMHIENLFDPYDNLKGALEYHGYLRRLFNDEKKQIAAYHDGPGSIQRQGMSITGDQ
                     YYNQVKSSQKKYKSIMLESPSVFGVSISFENGTILKTHFYNSAAYMKFEFYNYADIYI
                     NKKNDSFMLDYEINSSVLYFPRTVIAFGTGLTFEDKGFKNGDYILRLGFPWRSLIVRL
                     GEENSFTFKEEYKEFFYYLKLKNSSAIVAAGMKFNDFKVSVTFDLLKKSFGSYISIF"
BASE COUNT         1037 a          795 c          543 g         1295 t
ORIGIN      
        1 cttttacctt cttcaacaat agcctgggct tgctgattag ctttttttat tatttcctca
       61 gaccttttgt tagcctggaa aataatttcc ttagctttct cttctgtctc aacaagtcta
      121 tctttgacct gctggtccac caaaagattc tgctgattgt cttcagcaga aagtatttct
      181 tcctctattt ttttagaatt tatatctagc tttgagtcaa ggaccacgta cttatttttg
      241 ataactttct tattatacaa tcaattcctc tcctccgccg cttcctatta ttatttctcc
      301 tgattcttca agtcttctta tagtggcgac tatcttctgc tgagcctcat caacatcgcg
      361 aactcttaca ggtcccatat agtcaagctc ttcctgtaca atctggcttg aacgcttgga
      421 catattttta aatatagtca acttaacttc ttcagaagct ccctttagag caatagtaag
      481 atccttatta tctatttctc ttattattct ctgaatagat ctgtcatcaa gttttactat
      541 atcctcaaat acaaacattc tctttcgtat ttcttccgaa agctttggat ctctttcaga
      601 aagtctgtcg aatatatttt tacttacaac cctatcaata ttattcatta tttctgcagc
      661 agtttctatt cctccaacct gagagaatgt ctgtgtagca aaagaagaaa gtctgtcttt
      721 catcttggtc tcaacatctt ttacaacatc aggagtcgct gaattcatta tggaaattct
      781 tttaacaact tctacctgaa gactttcatt aaggttggtt attacctgag aggctatagt
      841 tgggctcaag taacatagta caagggcaat agtctgcgga tgttcattct gaagcacatt
      901 gaccatctga gcaacatcaa tcctcctaag aaaatcaaaa ggcctcactt gcaagcttga
      961 aacaagcttt tctattattt caattgcttt ttctggtccg aaagcatctt caagaagctt
     1021 acgggcatag tctattccac ccttatttat gaattctttt atcttagcaa actcaaaaaa
     1081 ctcagaactc acagagttct tcaaactttc ctctatcttt cctagattgg caatttccaa
     1141 tgtgagctgt tcaacatcgg aatcgggaag ttttttgagg atggttgcag ctctgtccga
     1201 tcccattaag accagcagca cagcagcttt ctttacacct gttatatctt cgtttttagc
     1261 cactgtaatc acctcattgt gtatttatcc aaatcttaat tatctcaaca acgtcattag
     1321 gtctcgagtc aactatagct tcaagtctct tctgcatttc ctggcttgga gaaagttcca
     1381 taggctcctc tttaacctct ccaacaactt ctttcacacg ttcttcaagc ttcttttttc
     1441 tctcttctat cagagtatag gttttctttc tgcgcatata taataaagca acatacaaga
     1501 ggaaaagcaa cattacaata gcaaccatta taagtataac gtaggttatc agtttccttc
     1561 ttctaaggtt ctctgcttcg atcttactct tttcattatc catttccctg ttaaaagtaa
     1621 gagaaagaac gcttatatta tcctgtcccg atcctgttgc agtggttata gaggatttaa
     1681 tattttttat gatctcctcg gtttcagtta caccggattt caagaaatct atatatattg
     1741 taaagctttt ctttgcaatt tctcccgttc tatcatcaat agtttttttg taaatctcgt
     1801 ttatatcata attagttatt actttcgatt tggagtatcc agtattctga tcagagcttt
     1861 ctgtctgata atcgtacgga ggaatatttg atgtcgttcc aggtgctccg ctgttgttag
     1921 aagagtttat agtctgttcg ctctcctgct gctggctgag tataataccg ctagacttgt
     1981 ttacaggttg aacagttctt tcctcctgtt caagtttctg ccagttaagt tcgacatcag
     2041 acatgactac aacatttccg aatccaaaaa cggtttgcat ggtctgttgt attttttttg
     2101 tgtagtaaga ttctatcatc tgcttcatct ggaatttagt gttggctgat cctatatcat
     2161 cattgtatat agaggagcta agatcgcgag aatagttatc aacaactctt acatttttgg
     2221 gttcaagccc ttgaacagca cctgaaataa gttctacaat accttttacc tgctcctgac
     2281 ttatttgagc acctgttgca agcgatatca ttacagaagc cttcggaatg gcattatctc
     2341 caactgtata gtatgtttta ggaggaataa caagatgcac tctggcaaaa gaaacaccag
     2401 ttatagctga tatagatctt tcaagctctc cttcaagtgc tatctgataa tttacctgtt
     2461 tatcaaaact agtagctcca aatccctggt tctgcaaaag ctcataccct ctggaattat
     2521 taccaagcac tccttctgaa gccagcttca tcctcaactc atacttttga ctatttgaaa
     2581 catagatgct cccattcgga ccagccttat actttacacc ctgctcgtca agctttgcta
     2641 taacctgccc tgcctgagtc tcattaagac cacttaccaa aagtgtatag tgtgggagag
     2701 atataacaac aaaaagaact atcataacta taattactga tagtgcatat attgtatatg
     2761 taattttctt ctttttttcg aggtttttcc accagccaag aatttttttc aaaaattctt
     2821 ccattttgtt ttttccaccc ctcagaatat cgatatgtac gaaccgaaag actttttaag
     2881 aagatcaaat gtcacagata ccttgaaatc attaaacttc atccctgcag caactattgc
     2941 cgaagaattc ttcagcttaa gatagtagaa aaattcttta tactcctctt taaatgtaaa
     3001 ggaattctct tctcctaacc ttacaataag actcctccag ggaaaaccca atctgagtat
     3061 ataatctcca tttttgaatc ccttgtcttc aaaagtcaaa ccggttccaa aagctatcac
     3121 tgtacgtgga aaatataaga ccgaggagtt tatctcatag tcaagcataa agctatcgtt
     3181 ctttttattt atatatatat ccgcataatt atagaactca aacttcatat aagctgcaga
     3241 gttataaaaa tgtgtcttta aaatagtccc attttcaaaa cttatactaa ccccaaatac
     3301 agaaggtgat tcaagcatta tgctcttata ttttttttga ctagatttaa cctgattata
     3361 atattgatca ccagttatag acattccttg acgctgtata ctccctggac catcatgata
     3421 tgcggctatc tgcttttttt catcattaaa aagccttctg agatatccat gatactccag
     3481 tgcccccttt aaattatcat atgggtcgaa taaattttca atatgcatat cagaggctgt
     3541 ctggtactta atctgcatta accctaaagc tttagagctg gagatagcat gggtataaaa
     3601 atcagattcc acatcaattt ggccaccaat catgcttctt tcctgagacc aaactgcctt
     3661 taaaaactca
//
LOCUS       DAOSHR010000199         3637 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_23572_length_3637_cov_5.302345, whole genome shotgun sequence.
ACCESSION   DAOSHR010000199 DAOSHR010000000
VERSION     DAOSHR010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 3637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3637
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23572_length_3637_cov_5.302345"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..689)
                     /locus_tag="PLU28_09375"
     CDS             complement(<1..689)
                     /locus_tag="PLU28_09375"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: protein motif:HMM:NF019073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008417 - fucosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0036065 - fucosylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HOG35307.1"
                     /translation="MKILHVIFDSNYTRTFYAFIQKNFRSCEHDFLIYGSISNDSVFM
                     GKEFHKITSFRKDSKKLRIIFSRADKIIFHGMFSESLNIFLFTHRNILKKAVWMIWGA
                     DIYPKNYSGKLKYFIFEKVIRTSVVRSFHTISALVPQDYEKLKEIYNTKTPYIQAFYP
                     NPVDFEMFSDYKVQSYKNQTLTVLCGNSADPANNHFQMFDMLGRFRNENIKIICPLSY
                     GGSKDYIQKTI"
     gene            complement(686..2098)
                     /locus_tag="PLU28_09380"
     CDS             complement(686..2098)
                     /locus_tag="PLU28_09380"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOG35308.1"
                     /translation="MAESARTFLKRFLGFSIGPLVSSVIGFFTVPITTWFIMPDEFGK
                     ASMYALASTFLAMVIFMGMDQAFAREFHARTDKKTLYFNAVLIPLSLSIIAGFFILIF
                     YKPFSMLLYASVQLDAMIMLAVSLPVSVFYRFNLLLIRMQERARLYSLLNILDRVLGF
                     TFLMLFIFLYEKSFKSIVLANFCSYISISAINVALTSDYFKKGVHIDKALLKVLVKFG
                     LPLIPTSLLAWVFESMDRIAMRQWSDFIQIGLYSGAFKIVTVLQLFSSAFNTFWAPTV
                     YRWYETNVEKDLFHKVSTVLFGMMSICFAFIVLFRNIIIMLLSKSYAGSAVLVPFLIY
                     IPIMYTTSETTGMGIGFTRKTSYSIIVSGVSAGINFIGNYLLVPKYGALGACISTAVS
                     YMSFFWVKTMISRKLWWKFNLKPYILNSFFMGVYSFITLFTNNFFLELPLALLILMYN
                     FPSIKYIYSVAFNKKTGYRP"
     gene            complement(2118..3491)
                     /locus_tag="PLU28_09385"
     CDS             complement(2118..3491)
                     /locus_tag="PLU28_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35309.1"
                     /translation="MRRFLVFCIVLLAGISTFSFEADFQFTQNFYDSVFPNKVRVALD
                     HSYNDFDFTAALYASNDGKYQSSMGDYYKGYYFFLDKGGLIFTPDKLSFSFGRLSHKD
                     EVDSPYSLFISSNYLPAVIGDIRFEDTVFFYNSRWIRLNVRSRYGYPDRGANYKTYGL
                     KFGNIRFGFQDSTVYVGRSFDEEHFLSPIPGFFTQYVNTSQGKPWSATGDENSILGFF
                     MDYRDSLSYYYWQILVDDLNVNRFMFPDRYQNPDKVAWSLGGNKQTVIGRIGFYHAGS
                     TKYTFEPSGGAAVDTKYGYTYYPDTVYTADSKERALEYEDNYIGFKYGENTAAFILNY
                     ANTFDRFSLYADCEYRVSGSKSPANPWQEYLTWDQGGQGTHFLDESILEKAFLSRIKL
                     STVLYGLKLSAGCDLGMIFNKLELTDIPPEYYNSAVNKIKYFKPSSKNESILKFHLSL
                     EYNLNLL"
BASE COUNT         1229 a          673 c          669 g         1066 t
ORIGIN      
        1 tctatagtct tttgaatata atctttactg cctccgtagg aaagggggca gattattttt
       61 atattttcgt tacggaacct gccaagcata tcaaacatct gaaaatgatt gtttgcagga
      121 tctgcagagt taccgcaaag tacagtcaag gtttgatttt tgtaggattg gactttataa
      181 tcactaaaca tctcaaaatc aacgggatta ggataaaaag cttgtatata gggtgttttg
      241 gtattgtata tttctttcag tttttcgtaa tcctgaggaa caagtgccga aatggtatga
      301 aaagaacgga ctacagaagt tcttataacc ttttcaaaaa taaagtattt caacttacca
      361 gaataatttt tgggatatat atctgctccc cagatcatcc atacggcttt tttaagaata
      421 tttctatgtg taaaaagaaa aatattgaga ctttcggaga acattccgtg gaatattatt
      481 ttatcagctc tggaaaaaat aattcggagt tttttggaat cttttctgaa agaagtgatt
      541 ttgtgaaact cctttcccat gaagacagaa tcgttagaaa tacttccata tatcagaaaa
      601 tcatgttcac atgaccggaa atttttttgt ataaaagcat aaaaggttct tgtatagttg
      661 ctgtcaaaaa tgacatgaag tattttcatg gtctgtaccc cgttttttta ttgaaggcta
      721 cggaatatat gtactttata cttggaaagt tgtacataag aatcagtaaa gcaagaggaa
      781 gctccaaaaa gaagttgttg gtaaaaaggg ttatgaaaga ataaactccc atgaaaaatg
      841 agttaagtat atatggtttc aaattaaact tccaccaaag ctttctggag atcatagtct
      901 ttacccagaa aaaagacata tacgaaaccg ccgtagatat acatgcgcca agagccccgt
      961 atttaggcac aagcagatag ttccctataa aattgattcc ggccgatact ccagaaacaa
     1021 tgatgctgta tgaggttttt cgtgtaaagc cgatacccat tccggttgtt tccgatgtgg
     1081 tatacattat agggatatat ataagaaagg gtaccagtac ggcagagccg gcatacgact
     1141 tgctcaaaag cataattata atgtttctga acagaacgat aaaagcaaag catatgctca
     1201 tcatgccaaa aagaaccgta cttactttat ggaaaagatc tttttctaca ttagtctcat
     1261 accatcgata cactgtagga gcccaaaaag tattgaaagc actgctgaaa agctgcaaaa
     1321 cggtaacaat cttaaaagct cctgaataaa gtcctatctg aataaaatca gaccactgtc
     1381 gcatggctat cctgtccatg gattcaaaaa cccatgccaa aagagatgta ggtatcaaag
     1441 gcagtccaaa tttgacaaga actttaagta aagctttgtc tatgtggact ccttttttga
     1501 agtaatcact tgtaagggct acatttattg cagatatgga gatataggaa caaaaatttg
     1561 caagcactat acttttaaaa cttttctcat acagaaatat aaaaagcatc agaaaagtaa
     1621 aacccaaaac acggtcaaga atattaagca gtgaatatag ccttgctctt tcctgcattc
     1681 ttataagaag aaggttgaaa cggtaaaata cagaaactgg cagggaaaca gccagcatga
     1741 tcattgcatc aagctggaca cttgcatata aaagcattga aaaaggttta taaaagataa
     1801 gaatgaaaaa accggctata atggaaagtg acagcggaat gaggacggcg ttaaaataaa
     1861 gagttttttt atccgtacgt gcatggaact cccttgcaaa agcctgatcc attcccataa
     1921 atatgaccat tgcaagaaag gtcgatgcaa gggcatacat cgaagccttt ccgaattcat
     1981 cgggcattat aaaccatgtt gttatcggta cagtgaaaaa gcctattaca gaagagacta
     2041 aaggaccaat ggaaaatcca agaaatcgtt taagaaaggt acgtgcgctt tcagccatct
     2101 ataaaaaacc gcctttttta aagtaagttt aggttgtact ccagtgaaag atgaaatttt
     2161 aaaatacttt cattttttga agaaggttta aagtacttta ttttattgac agcactatta
     2221 taatactcag gcggtatatc tgtgagctca agcttattaa aaatcattcc aagatcacag
     2281 ccggcactaa gcttcaaccc ataaagaact gttgaaagct ttatcctgct caaaaaagct
     2341 ttttcaagaa tactttcgtc cagaaaatgg gttccctgtc ccccctgatc ccaagtgaga
     2401 tactcctgcc atggattggc gggtgatttt gaaccagaaa ctctatattc acagtcagca
     2461 taaagactga aacggtcaaa agtatttgca tagttcagga taaatgcagc ggtattttct
     2521 ccatatttaa agcctatata attatcctca tactctaggg ctctttcttt ggaatcggca
     2581 gtatatacag tatccggata gtaggtatat ccgtattttg tatcaacagc cgctcctccg
     2641 gaaggttcaa aagtgtattt ggtcgaaccg gcatgataaa aacctattct tcctataact
     2701 gtctgtttgt ttccacccaa agaccaggct accttgtccg gattctgata tctgtctgga
     2761 aacatgaagc ggttgacatt aaggtcgtct acaagaatct gccagtaata ataagaaagg
     2821 ctgtctctgt aatccataaa aaaacccagt atggagtttt cgtcacctgt tgcgctccat
     2881 ggctttcctt gggaggtatt tacgtactgt gtgaagaatc cgggaattgg acttaaaaaa
     2941 tgctcctcat caaaacttct tcccacatat actgtcgaat cctgaaatcc aaaacgtata
     3001 ttgccgaact ttagtccata agtcttataa ttagctcccc tgtcaggata gccgtatctt
     3061 gaacggacgt tcaggcgtat ccatcttgaa ttgtagaaaa aaacagtatc ttcaaaccgt
     3121 atatcgccga taaccgccgg cagatagttt gaagaaatga aaagcgagta aggtgaatca
     3181 acctcatcct tatggcttaa tcttccgaat gagaatgaaa gcttatccgg agtgaatatg
     3241 agccctccct tgtcaagaaa aaagtagtaa cccttgtagt aatctcccat agaagactgg
     3301 tactttccgt cattgcttgc ataaagggct gcagtaaaat caaagtcgtt gtatgaatga
     3361 tccagagcta ctcttacttt gtttggaaag acagaatcat aaaaattttg cgtaaactga
     3421 aaatcagctt cgaaagaaaa tgtagaaatt ccggcaagca gaacaataca aaaaactaaa
     3481 aatcttctca tcttttttcc tccatgttca actgtttgat aagttcattg cgaaatcttt
     3541 cttctttttc gctgttccag aaaaaatcat ccctatatac ggcaatatga tttaaaatac
     3601 gttcatactc tcttttttgt gagcttctca taaaaac
//
LOCUS       DAOSHR010000200         3609 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_23843_length_3609_cov_3.929657, whole genome shotgun sequence.
ACCESSION   DAOSHR010000200 DAOSHR010000000
VERSION     DAOSHR010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 3609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3609
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23843_length_3609_cov_3.929657"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="PLU28_09390"
     CDS             complement(<1..279)
                     /locus_tag="PLU28_09390"
                     /inference="COORDINATES: protein motif:HMM:NF024636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2TM domain-containing protein"
                     /protein_id="HOG35310.1"
                     /translation="MDEKQKFEIAKKKVQDIKDFYVHLMVYVVVNAFLFIINMLTSRS
                     YLWVVWPMLGWGIGVFFNWLSVFGMNGMLGRDWEEKKVEEIMKKMDDKN"
     gene            complement(477..677)
                     /locus_tag="PLU28_09395"
     CDS             complement(477..677)
                     /locus_tag="PLU28_09395"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HOG35311.1"
                     /translation="MKYTYTVNDMACGHCKMHIEKALKALEGVESFNVDLKTKKVEVQ
                     TELNPELIEGAIKDAGYTPEKV"
     gene            complement(691..2853)
                     /locus_tag="PLU28_09400"
     CDS             complement(691..2853)
                     /locus_tag="PLU28_09400"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HOG35312.1"
                     /translation="MENEYKKSFFVQGMTCASCVRNVENALKKMPGVKYVSVNLATEQ
                     AYLISEKDVSFQEIKKTVEESGYVALSKAPDGSAIEKRFLRSRKNFILSISVTAVLML
                     LMILHMSGVHIPYFNWYEIILAGFVLFFTGRDTFKGAWIALSHKHTNMDTLVSIGAVA
                     SYITVWLELSGLNILSFASISAMLVSLHLTGRYIEARLKQKASEEIKAIIGLQAQTAN
                     IQTAQGILKVPIQTVKVGTQVIVRQGERIPLDGKIILGEGYADESMITGEPLPVSRKS
                     GDNVIGGSILSGGSITVEVLKTGEDTFLSQMIKLVQQVQGSKVPIQAFADRITLYFIP
                     IVLSLAVIASIAWFFGYQSMQPFMMKLSSFFPWINYDMGALSTAVFVFVSTMVIACPC
                     ALGLATPMALLAGTSSAARKGLIIRNGEAIQSSKNINVLLLDKTGTVTQGKPKVVQID
                     MDPEDLNKIANIEKNSIHPLAKAIWEYNLPNYSEISIEDVREEVGHGISGRHLSDTYF
                     IGKPEYPSKYTELLNQGYTVLQALKNGQNIGCIAISDPIKEDSAHAVGELKSMGIKVV
                     MVTGDDEKTAAIISKKVGIDEFEAGVKPEGKADIVKKYQIQGYKVGMVGDGINDAAAL
                     KSADIGIAVGTGTDLAIESADIVIVKGELSKVADAINISKITFLKIKQNLFWAFFYNV
                     VAIPLAMTGVLHPIISELAMTFSSINVILNSLSIRKKI"
     gene            complement(2868..3146)
                     /locus_tag="PLU28_09405"
     CDS             complement(2868..3146)
                     /locus_tag="PLU28_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensing transcriptional repressor"
                     /protein_id="HOG35313.1"
                     /translation="MNKMHHEEALKTLKTAKGQLESTIKMIEENRYCIDISKQVLAVT
                     ALLKKANIQILKKHLKSCVLDAALSKDSTEISVKIKELEDTLEYINRV"
     gene            3297..>3609
                     /locus_tag="PLU28_09410"
     CDS             3297..>3609
                     /locus_tag="PLU28_09410"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF014228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HOG35314.1"
                     /translation="MKKILCLTGAGISKASGIPTFTEMEDIRIKLSRTYYRSNPDDFF
                     SVLIKMKDNIESAQPNPAHIALARFKVPIVTMNIDGLHQKAGSTEVIELHGNLDFVEC
                     KK"
BASE COUNT          997 a          759 c          614 g         1239 t
ORIGIN      
        1 atttttatcg tccatttttt tcattatttc ttcgaccttt ttctcttccc agtcacggcc
       61 cagcataccg ttcatgccaa atacggaaag ccagttgaag aaaactccta ttccccagcc
      121 gagcatgggc catacaaccc ataagtagct cctggaagtc agcatgttga ttataaaaag
      181 gaaagcattc acaacaacgt agaccatgag atgtacataa aaatccttga tatcctgaac
      241 ctttttcttt gcaatttcaa acttctgctt ttcatccata agagataaca ccccctgata
      301 atttatacag tcttattata caacaaagaa tatttttttt tatttcctct ggacaaagtg
      361 cattttttaa aggacaacca acatattaaa actgacctcc gaaagttggt tttctcatcc
      421 aactttggaa agtcagttca aagtataaat acgtcgtttt taaatttttt atggttttat
      481 actttttccg gggtatatcc ggcatctttt atagcgcctt caatcagctc tggattaagc
      541 tctgtctgta cttcaacttt cttggtttta agatcaacat taaagctttc aacgccttcc
      601 aacgccttta atgctttttc aatgtgcatc ttgcagtgtc cgcatgccat atcgtttaca
      661 gtatacgtgt acttcataat ccacctccgt ttaaattttt tttcttatgc taagcgaatt
      721 aagtataacg tttattgagc tgaaggtcat tgccagttca gaaattatgg ggtgaagcac
      781 acctgtcatt gcaagaggaa tagcaacaac attatagaaa aaagcccaga aaagattttg
      841 ttttatcttt aaaaacgtaa tttttgatat atttatggca tcagctactt ttgacagttc
      901 accttttaca attacaatat ctgcgctttc tatggcaaga tctgttcctg ttccgacagc
      961 tattcctata tccgctgatt tcaaggctgc tgcatcgttt attccatctc cgaccatccc
     1021 tactttatat ccctgaatct ggtatttttt aactatatct gcctttcctt caggcttgac
     1081 tcctgcttca aactcatcta tccctacttt tttgcttatt atggctgctg ttttttcatc
     1141 atctccggtt accataacta cttttattcc catactctta agctctccaa ccgcatgggc
     1201 agaatcttcc tttattggat cagaaatagc tatacagcca atattctgac cgttttttaa
     1261 agcttgcaaa acagtgtagc cctgatttaa aagttctgta tactttgacg gatattcagg
     1321 cttgcctata aagtatgtgt cgcttaaatg tctgccagag atcccatgtc cgacttcttc
     1381 acgtacatct tcaatagata tttctgaata gttgggaagg ttatactccc aaatggcttt
     1441 agccagcgga tgaattgaat ttttttctat gtttgctatt ttattaagat cttcaggatc
     1501 catatcaatt tgcactactt tcggttttcc ctgagttacc gttccggttt tatctaaaag
     1561 cagaacattt atgtttttgg aagattgtat agcttctccg tttcttatta taaggccttt
     1621 gcgtgccgct gaggaagttc ccgcaagaag tgccatggga gtagcaagcc ccaaagcgca
     1681 tggacaggct attaccatag tcgatacaaa tacaaacaca gccgttgaaa gcgcacccat
     1741 atcgtagttt atccatggaa aaaaagagga gagcttcatc ataaaaggct gcatactttg
     1801 atatccgaag aaccaggcta tactggctat gaccgccaat gaaagaacta taggtataaa
     1861 gtacagggta atcctgtcag caaatgcctg aataggaact tttgagccct gaacctgttg
     1921 aaccagtttt atcatctgac ttaaaaatgt atcttctccg gttttgagca cttcaaccgt
     1981 tattgagcca cctgaaagta tacttcctcc tatgacgtta tcgccggatt ttctacttac
     2041 cggaagtggt tctcccgtta tcatagactc atctgcatat ccttcaccaa gaataatttt
     2101 tccgtccagc ggtattcttt ctccctgtct tactataact tgtgttccga ctttaacagt
     2161 ctgaatagga acttttaata tcccttgtgc cgtctggatg ttggctgtct gtgcctgaag
     2221 tcctatgatc gcttttatct cttcagaagc cttttgtttt aatctggcct caatatatct
     2281 tccggtaaga tgaagagaaa caagcatggc ggatatactt gcaaaggaaa gtatattaag
     2341 tcctgagagc tcaagccata ccgtaatgta tgaagcaacg gctccaatag atacaagagt
     2401 gtccatgttg gtatgcttat gacttaaggc aatccacgca cccttgaaag tatctctgcc
     2461 tgtaaaaaaa agtacaaacc ccgccagtat tatttcatac cagttgaaat aaggaatatg
     2521 tacaccgctc atatgaagta tcatcagtag cataaggact gcggtgacag agatggagag
     2581 tataaaattt tttctgctcc ttaaaaatcg tttctctatt gcagatccgt cgggtgcttt
     2641 ggataaggct acatatcctg actcttcaac agtttttttt atctcttgga aagaaacatc
     2701 tttctcggaa ataaggtatg cctgttctgt tgccagattg acggatacat acttgactcc
     2761 cggcatcttt tttaaagcat tttccacatt tctgacacat gatgcgcatg tcattccctg
     2821 aacgaaaaag ctttttttat actcattttc cattttgcac ctccgcttta taccctgttt
     2881 atatactcta aagtatcttc aagttctttt atttttactg atatttctgt ggaatcctta
     2941 gaaagagctg catctaaaac acagctttta agatgctttt ttagtatctg aatgttggct
     3001 tttttaagga gggctgttac tgccaatacc tgtttggaaa tatctatgca gtacctgttt
     3061 tcttctatca tctttatagt tgattcgagc tggcctttcg cagttttcag tgtttttaac
     3121 gcttcttcat gatgcatttt attcatattt cctccatatc ataccaccct acctaggggg
     3181 gatatatata tcctaccttt ttagtattat tttcatgtta cagagaagtt ttttttgaca
     3241 aagaaatttt ccattgtact ctttttttct gctataatct ttatgaggtg atatttatga
     3301 aaaaaatttt gtgtcttaca ggagcaggaa tatctaaggc aagcggaatt cctactttca
     3361 ccgaaatgga ggatatccgt atcaagctga gccgcaccta ctatagatcc aatcccgacg
     3421 attttttttc tgttcttata aaaatgaagg ataatattga gtctgctcag cccaatccgg
     3481 ctcatatcgc cttagcaagg tttaaagtac ctatagtaac gatgaatatt gatggtcttc
     3541 atcaaaaagc agggagcaca gaggttatag aactgcatgg taatcttgat tttgtggagt
     3601 gtaaaaaat
//
LOCUS       DAOSHR010000201         3573 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_24191_length_3573_cov_5.330301, whole genome shotgun sequence.
ACCESSION   DAOSHR010000201 DAOSHR010000000
VERSION     DAOSHR010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 3573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3573
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24191_length_3573_cov_5.330301"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="PLU28_09415"
     CDS             <1..542
                     /locus_tag="PLU28_09415"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOG35315.1"
                     /translation="KNGIKKYVLEVLKENEKAFNIYTQKGFQVSRGFNCYRVAKEDYS
                     SAKKLKDTDLRVEKLSYINWREFRSFWDVQPSWQNSESSVNRSEDKKAFLGVYKKQEL
                     MAYAVLYPSTGDLAQLAVKKNFRGNGAGTLLLKACLAVSDKPLSILNVEDSDKDINGF
                     LNSFGCKLFTMQHELIKKI"
     gene            555..908
                     /locus_tag="PLU28_09420"
     CDS             555..908
                     /locus_tag="PLU28_09420"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HOG35316.1"
                     /translation="MLYDEFISWREEMNKKILEKGTTNTKRFFNIDGAVYQPGAISTK
                     YKELMGLVASMVLRCEGCIDYHIMTCIENGVTDEEFFEAFDIALIVGGSIVIPHLRQA
                     VSFLEDARRHKLSDK"
     gene            905..1387
                     /locus_tag="PLU28_09425"
     CDS             905..1387
                     /locus_tag="PLU28_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35317.1"
                     /translation="MTSSGDLIELHKHLNFLFSISEDTFKIIASKQNRVSYGNYSGHY
                     TKIDGRYEYQKYPLPVITVADKGDIGFDLFCVWFEFFIDPLSLRYEKITKISSLYRTE
                     VYTQDCAKDIKLSNNPREFGETMASITDRKIGISVYIDYSAPESIVHVFSFICKILEI
                     "
     gene            1413..2576
                     /locus_tag="PLU28_09430"
     CDS             1413..2576
                     /locus_tag="PLU28_09430"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF003705.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HOG35318.1"
                     /translation="MIDRDEMEELFHKRFGQVPFKKEFFFSPGRVNLIGEHIDYSGGF
                     VFPMAISLGISACVSFNDSDIIRIFSDNATDEVHIDIKKEIAPGAPVWGDYPKGVIKL
                     IIEEGYSFKGFDMVIKSDLPQGAGLSSSASLEVLIAYIIFSSILEESKIDMLYIARLC
                     QKAENMFVGVKCGIMDQFAVACSRKGFAVKLDCASMAYEYVPADMGEYSLIIMNTNKK
                     RELSDSKYNQRRDECDQAILELSRKYGEKALTGYTLGQIEEAIKDPVIKKRCRHAITE
                     NQRVLKAVESLKCGDIMNFSNLMIHSNISLKYDYEVTGFELDTIVDSALKQKGCLGAR
                     MTGAGFGGCAIALVIKDLSDEFISEVSIQYAEKTGIQAGFYTISSDDGTRKVY"
     gene            2585..3520
                     /locus_tag="PLU28_09435"
     CDS             2585..3520
                     /locus_tag="PLU28_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35319.1"
                     /translation="MRIFQGSYQEFDSIFIENNHIKMTIIPEIGGKIASIIYKPQAFE
                     ILFQPSEPYRMPIYGDSFEKYDTSGIDEMFPNIDISDYPYGISNMVTLKDHGELWSVP
                     WGASIDRSRVITQVAGTELEYSFKRIVEVKNNQIFLDYVVTNNSKKSILGIWAFHGLL
                     ACDEMTQIFLPTDKVINVHDSSVLGKAETLHSFPVTKDLQARDYRLDRIGSPLLKKAE
                     KYYVCGKLKTGQASLTLNKNQLLCKMNFNEKKIPYLGVWINEGGYKNQYNCALEPSTG
                     YYDSIMRAYNNNAVMEILPGRRISWGLELEIMPNY"
BASE COUNT         1206 a          570 c          780 g         1017 t
ORIGIN      
        1 aaaagaacgg tataaaaaaa tatgttcttg aagtactcaa ggaaaatgaa aaagctttta
       61 atatatatac tcaaaaaggc tttcaggttt cacgtggctt taactgttat agagtggcaa
      121 aggaagatta cagttctgca aaaaagttaa aggatacgga tctgagggtt gaaaagcttt
      181 cttatataaa ctggagagag tttcggtctt tttgggatgt tcagccttca tggcagaact
      241 ctgagagttc tgtcaacaga agtgaggata aaaaagcttt tcttggggtt tataaaaaac
      301 aggagcttat ggcctatgct gttctttatc cttcaaccgg agatcttgca cagcttgccg
      361 tgaagaaaaa cttcagaggc aacggtgccg gaacactcct gcttaaagca tgtctggccg
      421 tttctgataa gcctttaagt attctgaacg tggaagattc agataaggac ataaacggtt
      481 tcctaaactc tttcggatgc aaacttttta ccatgcagca cgaacttata aaaaaaatat
      541 aaggagcgac aaatatgctt tatgatgaat ttataagctg gcgtgaagag atgaacaaaa
      601 agattttgga aaaaggaacc accaatacga aaagattttt caatatcgac ggagcagttt
      661 atcagcccgg agcaataagc acaaagtata aagagcttat gggacttgtg gcttcaatgg
      721 ttttaagatg cgaaggctgt atagactatc atataatgac gtgtatagaa aacggagtta
      781 cagacgaaga gttttttgaa gcatttgata ttgctctcat tgtaggaggg tcgatagtaa
      841 tacctcattt aagacaggca gtaagctttc ttgaggatgc gcgcagacat aagctgtcag
      901 ataaatgaca agttccggtg acctgataga gcttcataaa catttaaact ttttgttctc
      961 tatatctgaa gatactttta aaataattgc ttcaaaacaa aacagagtaa gctacggtaa
     1021 ttattccggg cattatacaa agatagatgg aaggtacgag tatcaaaaat atcctcttcc
     1081 tgtgattaca gttgcagata aaggtgatat agggtttgac ttgttttgtg tgtggtttga
     1141 gttttttatt gatcctttaa gcttgaggta tgaaaaaatt accaaaatat cttcacttta
     1201 tcgtacagag gtatatacac aagattgtgc aaaggatata aagttatcaa ataacccccg
     1261 ggagtttgga gaaacaatgg cctcaatcac ggacaggaag atagggataa gtgtatatat
     1321 cgattattca gctccggaaa gtatagtaca tgtattttct tttatatgca aaattttaga
     1381 aatatagctt ttgcaaaaag gaagtgaggt acttgataga cagagatgag atggaagaac
     1441 tttttcataa aagattcgga caggtgccat tcaaaaaaga gttcttcttt tcccccggaa
     1501 gagtaaatct tataggagaa catatagact acagcggagg ctttgttttc cctatggcta
     1561 tatctcttgg gataagcgcc tgtgtttcat tcaatgactc ggatattata agaatatttt
     1621 ccgataatgc tacagatgaa gtacatatag atataaaaaa agagatcgct ccgggagctc
     1681 ccgtatgggg agattacccc aagggcgtta ttaaacttat aattgaagaa ggatacagct
     1741 tcaaaggatt tgatatggta ataaaaagcg atcttcccca gggagccgga ctatcttctt
     1801 ccgccagttt ggaagtgctg atagcataca ttatatttag ttcaatttta gaagaatcaa
     1861 agatcgatat gctttatatt gcccggctat gccagaaagc tgaaaatatg tttgtcggtg
     1921 taaaatgcgg aataatggat cagttcgctg ttgcttgctc aagaaaaggg tttgctgtta
     1981 aacttgactg tgcaagtatg gcttatgagt atgttcctgc tgacatggga gagtacagtc
     2041 ttattataat gaacacaaat aaaaaaaggg agctgtccga ctcaaagtac aaccaaagaa
     2101 gggatgaatg tgatcaggca attctggagc ttagcagaaa gtatggggaa aaagcattga
     2161 ccggatatac tcttggacag attgaagaag ctataaaaga tccagttata aagaaaaggt
     2221 gcaggcacgc cataacagaa aatcaaagag tgctaaaagc agttgaaagt cttaaatgcg
     2281 gggatataat gaatttttca aacctaatga tacattccaa tatttccttg aaatatgatt
     2341 acgaagtaac gggttttgaa cttgatacta tcgttgattc tgcacttaaa cagaaaggct
     2401 gcctaggagc cagaatgaca ggggcaggtt ttggcggttg tgccattgct ctggtgatta
     2461 aagatctttc tgacgagttt ataagtgaag taagcataca gtacgcagaa aagacaggaa
     2521 tacaagccgg tttttataca atcagcagtg atgatggcac tagaaaagtg tactgaggtg
     2581 tagtgtgaga atatttcagg gaagttatca ggaatttgac agtattttta ttgaaaacaa
     2641 ccatataaaa atgacgatca ttccggaaat aggcggcaaa atagcttcga tcatctataa
     2701 gccacaggct tttgaaattc tttttcagcc gtcagagcct tacagaatgc caatttatgg
     2761 agacagtttt gaaaaatacg atacttcggg aatagatgag atgttcccta acatagacat
     2821 atcagattac ccttatggta tttcaaatat ggttacattg aaagatcacg gcgaactatg
     2881 gtctgttcct tggggagcga gcatagaccg ttcacgtgtt ataacccaag ttgcaggtac
     2941 tgagcttgaa tattctttta aacgtattgt tgaagtaaaa aacaaccaaa tttttcttga
     3001 ctacgtagtt acaaataatt caaaaaagag tatattaggc atatgggctt ttcatggtct
     3061 gcttgcctgt gatgaaatga cacagatctt tcttcctacc gataaagtta taaatgtaca
     3121 tgacagttct gttcttggaa aagcggagac acttcacagc tttccggtga ctaaagatct
     3181 gcaggcaaga gattacaggt tggacagaat aggttcaccg ctacttaaaa aagctgaaaa
     3241 atactatgtt tgcggaaagc taaagacagg acaggcatct cttacactta acaagaacca
     3301 acttttgtgc aagatgaact tcaacgaaaa aaaaattccg tatcttggag tttggataaa
     3361 cgaaggtggg tacaagaacc agtacaactg tgcgcttgaa ccatctacag gttactatga
     3421 ttctataatg cgggcctata ataacaatgc tgtcatggaa attcttccag gacgcagaat
     3481 ttcatggggt cttgaactgg agataatgcc gaattattaa aaatccccgt tctcagaagc
     3541 agtagaacgg ggatctgttt ttatagaata cag
//
LOCUS       DAOSHR010000202         3571 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_24216_length_3571_cov_4.329636, whole genome shotgun sequence.
ACCESSION   DAOSHR010000202 DAOSHR010000000
VERSION     DAOSHR010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 3571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3571
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24216_length_3571_cov_4.329636"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            172..1371
                     /locus_tag="PLU28_09440"
     CDS             172..1371
                     /locus_tag="PLU28_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HOG35320.1"
                     /translation="MNPVMQRAKELEPLITSFMQRLIRARSFSSEEKEVTDLICAQMN
                     QNGFDEVYKDGLGNVIGRIGNGKTKIAIDAHIDTVEEGDVSLWTKPVFEGFRDGGYIY
                     GRGASDQKGGICSAVYAAKIIKEMDLCGDFTLYVTATVMEEDCDGMCWRYIIEKDGIK
                     PDIVLITEPTSLNIYRGHRGRLEFRIRTSGVSAHASAPQRGQNAIYKMAKIINEIEKL
                     NECLEEDEFLGKGTIVVSQIFFTSPSANAVPDSCEIQLDRRIIPSDSKEKIFGQIRQA
                     IAKAGVDGATIKELTYEKPSYTSQVYPVEKFFPSWVISEQSMAVKAAVHAYTELFGTQ
                     PVIDKWTFSTNGTVTAGVYNIPTIGFGPGEEKYAHAPDERVEIDHLIKACAFYASFPM
                     ACMKFIT"
     gene            1392..>2146
                     /locus_tag="PLU28_09445"
     CDS             1392..>2146
                     /locus_tag="PLU28_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HOG35321.1"
                     /translation="MSTFFRGRHFIDTQDYTREEIDIMLELSKDLKRKFAFSELTPYL
                     LYKTLFLIFFDQSTRTRNSMEAGIAQLGGNGIYLSPDKMQLSHGESPKDTGIILSRFG
                     DAIGIRHCAFGQGNRYIRNIADHSKAPVMNLQDDVYHPFQALADLMTIQERFGNNLRG
                     LKVGISWAYAESHLKPLSVPQSQILLFTRYAMDITVAAPKGFDLMPDIVEQANKNAYE
                     NNCKITFTESMDEAFEGAHIVFPKNWGGFFISQ"
     assembly_gap    2147..2246
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2247..3563
                     /locus_tag="PLU28_09450"
     CDS             <2247..3563
                     /locus_tag="PLU28_09450"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOG35322.1"
                     /translation="MYDLAILNGDVYLNGEFVNTNVYIKNGRVALIGEELMRAAEVYD
                     AYNKLVLPGFIDPHVHFSLKTERGMSADTFFSGSISAAFGGITTFIDFLDPIRQDREL
                     DPYFENRMNLASDSVVDYSFHSCLGNFRGDVSKVLKKIKNFGIPSVKVFTTYSQSDRM
                     CQDYVIDEIMSFSRKQKILLMAHAENDSLIRKEQDIEVKDLYESRNCLCEATEVVKLA
                     QMNEYHDGNLYIVHTGCGEIIQILREKYCKLVSKSILLESAPHYFIFDNNVYNMENGF
                     LFTMTPPLKPKKQRSLLWENFDAISVIGTDHCPFRVAEKKQKYVSEIPMGVGGVEHSF
                     EIMYNILGKKVIDKFTINPAKIFGLFPQKGTIFPSSDADIVIFDPNKEHTIKESHSGS
                     DYDIYKGITVKGAVECTICRGKFVVKNNIFTGKRGNGHFVKRNEYE"
BASE COUNT         1160 a          592 c          794 g          925 t
ORIGIN      
        1 atgggttgaa cagtagaata agacggttgc ccacctaaat gcacagaaag aacaagccta
       61 ctggcgtgag caacaggaga gaacttttga aacggataag cagataaaag agtacagaaa
      121 atcacatgaa acagttctca gaaagataat ggaatattaa ggaggggtaa gatgaatcct
      181 gtgatgcaaa gggcaaaaga gttggaaccc cttataacca gttttatgca gagactgata
      241 agagccagga gtttttcttc agaagagaaa gaggttacag atctgatttg cgcacagatg
      301 aatcagaatg ggtttgatga agtttataaa gacggtctgg gaaatgtgat aggaagaatc
      361 ggcaacggga agaccaaaat agcaatagat gcgcatattg atacagtcga agaaggggat
      421 gtgtctttat ggaccaagcc cgtattcgaa ggctttcgtg acggagggta catctacgga
      481 aggggtgcaa gtgatcagaa aggtggtata tgcagtgcgg tctacgccgc aaagataatt
      541 aaagagatgg atctttgcgg tgattttact ttatatgtga cggccactgt tatggaagaa
      601 gactgtgacg ggatgtgctg gagatacata atagaaaagg acggcataaa acctgatatt
      661 gttttgataa cagaaccgac ttcacttaat atttacagag gacacagggg aagattggag
      721 ttcagaatca gaaccagcgg ggtgagtgca catgccagcg ctccacaaag aggacaaaac
      781 gccatataca aaatggcaaa aattataaac gaaattgaga agttaaatga atgccttgaa
      841 gaagatgagt tccttggcaa aggaaccata gttgtatcac agattttttt tacctcacct
      901 tcagcaaatg ccgtgccaga cagctgtgaa atacagcttg acagaaggat tataccttca
      961 gattccaagg aaaaaatatt tggacagatc aggcaggcca ttgcaaaagc cggggttgac
     1021 ggtgcaacaa taaaagaact tacctacgaa aagccatcat atacttcgca ggtatacccg
     1081 gtggaaaaat tctttccgag ctgggttatt tctgagcaaa gcatggcggt aaaggccgca
     1141 gttcatgcat acacagaact ttttggtacg cagccggtta tagacaaatg gacattcagt
     1201 actaacggta cagttaccgc cggtgtatac aatattccaa ccatcggatt cggacctgga
     1261 gaagagaagt atgcacatgc tcctgacgag agggtagaga ttgatcatct gattaaggca
     1321 tgtgcttttt acgcatcttt tccaatggcg tgcatgaaat tcataaccta gagggagcag
     1381 aggaggttga aatgtcgact tttttccgtg gcaggcactt tatagatacg caggattata
     1441 caagggaaga aatagatata atgcttgaac tttccaaaga tctaaaaaga aagtttgctt
     1501 tttcagaact tactccttat cttttgtaca aaactctgtt tctcatcttt ttcgaccaat
     1561 caacaagaac gcggaacagt atggaggcag gtatagccca gcttggagga aacggaatat
     1621 acctttcacc cgacaagatg cagctctctc acggagaatc gccaaaagat acaggaatta
     1681 tcctatcccg ttttggtgat gctatcggaa taaggcactg tgcattcggg cagggtaaca
     1741 gatatataag aaatatagcc gatcattcca aagccccggt tatgaatctt caggatgatg
     1801 tataccatcc ttttcaggct cttgcagatc ttatgaccat tcaggaaaga ttcgggaata
     1861 atcttagagg tcttaaggtc ggtataagct gggcttatgc agaatcacac cttaagcctc
     1921 tttctgttcc acagtcacag atacttcttt ttacaagata tgcaatggac ataacagtag
     1981 ccgctcccaa aggctttgat cttatgcctg atattgtaga gcaggcaaat aaaaatgctt
     2041 atgaaaacaa ctgtaagata acgtttactg aatccatgga tgaggctttt gaaggtgcac
     2101 atattgtttt tcctaagaac tgggggggat tttttatttc acaagannnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnatgt atgatcttgc cattttaaac ggagatgtct
     2281 atttaaacgg agagtttgtc aacacaaatg tttatattaa aaatggaaga gttgcgctca
     2341 taggtgaaga actaatgagg gcagcggaag tttacgatgc atacaataag cttgttcttc
     2401 ctggatttat agatccacat gtccattttt cacttaaaac ggaaagagga atgagtgccg
     2461 atacattctt ttctggttct ataagtgctg cttttggagg gataacgaca tttatagatt
     2521 ttcttgatcc aataagacaa gacagagagc ttgatccgta ctttgagaac cgtatgaatc
     2581 tagccagcga cagtgttgta gattactcat ttcattcatg tttgggaaat ttcagaggtg
     2641 atgtatccaa agttttaaaa aaaataaaaa attttggtat tccgtccgtg aaggtgttta
     2701 caacatacag ccagagcgac agaatgtgtc aggattacgt tattgatgaa ataatgagct
     2761 tttcacgtaa acaaaagata cttcttatgg ctcatgccga aaatgattcg cttataagga
     2821 aggagcagga tattgaagtt aaagaccttt atgagtcaag aaactgtctg tgcgaagcaa
     2881 ctgaggttgt aaagctggca cagatgaatg agtatcatga tgggaatctt tatatcgtac
     2941 ataccgggtg cggagaaata atacaaatac ttagagaaaa gtactgtaag cttgtgagca
     3001 aaagtatact ccttgaaagt gcccctcatt actttatttt tgacaacaat gtatacaaca
     3061 tggaaaacgg ttttcttttc acgatgacac cgccgttaaa acccaaaaaa caacgttcgc
     3121 tgctttggga aaatttcgat gctatatctg taataggaac agaccactgt cctttcagag
     3181 tagcagaaaa aaaacagaag tatgtttctg aaataccgat gggtgttggc ggagtagagc
     3241 attcttttga gataatgtac aatattcttg gaaaaaaggt gattgacaag tttacgatca
     3301 accccgccaa aatttttgga ctttttccac agaaaggaac aatttttccg tcttcagatg
     3361 cagatattgt aatatttgac cctaataaag agcatactat aaaagaaagt cacagcggct
     3421 ctgattatga tatatacaaa ggtataactg taaaaggggc agttgaatgt accatatgca
     3481 gaggaaagtt tgtggttaaa aacaatattt ttacgggcaa aagaggaaac ggacattttg
     3541 tgaaaaggaa tgaatatgaa tgaaggccat a
//
LOCUS       DAOSHR010000203         3570 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_24227_length_3570_cov_4.450640, whole genome shotgun sequence.
ACCESSION   DAOSHR010000203 DAOSHR010000000
VERSION     DAOSHR010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 3570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3570
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24227_length_3570_cov_4.450640"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..748
                     /locus_tag="PLU28_09455"
     CDS             <1..748
                     /locus_tag="PLU28_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ExsB family protein"
                     /protein_id="HOG35323.1"
                     /translation="AQMGLRLFFIPGEDEQTALMYHGPNCNSCTKNIKMGQVRKFVYK
                     GIIASGANLSDSWGKTGIKFMDGFYSPLIGLEKVEIRKILEYYGVTIPKIGENYNREG
                     CKLKHLLKMNVSKDFHARAVCFSNEVIHDILDQFEYERSIANVKIIGPLSKNIALVNI
                     KPFPDKAIQDEIFSVLSAESSINEVWFADKPIELEILANPGIYNNMDSKYWVLNGRLA
                     PEFSCPVTAVWKKSRNNKLHTFAVTGFRYI"
     gene            841..>1097
                     /locus_tag="PLU28_09460"
     CDS             841..>1097
                     /locus_tag="PLU28_09460"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOG35324.1"
                     /translation="MRLKYFILGYIFLSATFLWAFEKINVAVTPAYVPYVLEIEQGKL
                     SGLSIDLWNEWEKQTGIEVEFVPVTLKEGLAMLKNNEIDVM"
     assembly_gap    1098..1197
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1198..2686
                     /locus_tag="PLU28_09465"
     CDS             <1198..2686
                     /locus_tag="PLU28_09465"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOG35325.1"
                     /translation="PAVLYYLNKYESLDLFKISEPLYTEKVRRGVPQGRQDLLQVVED
                     GFAKIPSKKVQDILKKWKGQTDPSNIMRQILYIGMVACGIIFAVFLLWSYFLKRLVDI
                     KTKELNEKNRELTYANNELVAINKELEHSFEQIEELSEKFEDLINMASVAFFDTINKD
                     ETFFKRLLETAMRIIPESDYGSISLVDGDQWTFVAAVGHDLEKLRLIPLKKSYLVFFC
                     EPVIVEKLRDQNRELPDGIYQKIEEASMPVRYSLIAPLVVNGEYLGNIDLDIDENSEK
                     AFSKDSIKILKAFSNIANAFLAFKRYSDVRSSFQKEVITSMVNILEVHDEYTKGHSSN
                     VAEISLNIAKKMMMSQEDMEKIFWAGLLHDIGKILIPTDILNKPAKLTYQEYEEVKKH
                     PAYGYDILSKSKNLREIADIVLYHHERYDGNGYPNKIKGVDIPLLSRIICVADSIDAM
                     YSKRSYRKPLTWEQINQELKDNTARQFDPDIIEVVLKMSKEEIFR"
     gene            2739..3023
                     /locus_tag="PLU28_09470"
     CDS             2739..3023
                     /locus_tag="PLU28_09470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35326.1"
                     /translation="MKKSFYASAVINGLCGGAVQLKDDAFYFMCQKLTVPEEYKQLRL
                     VYSDISSINFKRVLWILPMTTIELKSGKTYRFFIFNQKGFIKSMNEMSKK"
     gene            3086..3550
                     /locus_tag="PLU28_09475"
     CDS             3086..3550
                     /locus_tag="PLU28_09475"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOG35327.1"
                     /translation="MDFFSEALAVMDSQMGYDVCISLATLSDSGPSVRVVDGYYHGGS
                     LYVVSHTASHKMKDIAKDPRVAGCYKIHQFWGKGVNMGNPKKPENLELARTLRKVFFK
                     FYDRHVNEDDPECCILRIVLSRAVVFSQDAKYIVDFQNRSAEKIAMKLDIIE"
BASE COUNT         1147 a          547 c          758 g         1018 t
ORIGIN      
        1 ggctcaaatg ggtctgagac tttttttcat tcccggggag gatgaacaga ctgccctgat
       61 gtatcatgga cccaactgca acagctgtac caaaaacata aagatgggtc aggtcaggaa
      121 gtttgtgtat aaaggcataa tcgcatcagg ggcaaacctt tcggacagtt ggggtaaaac
      181 cggaataaag tttatggatg gtttttattc tccgcttata ggcttggaaa aagttgaaat
      241 aagaaaaatt cttgaatatt acggagtaac cattcccaaa ataggcgaga actacaaccg
      301 cgagggatgc aagctgaagc atcttcttaa gatgaatgtg tcaaaagatt ttcatgcaag
      361 agcggtctgt ttttccaacg aagttattca tgacatactt gatcagtttg agtatgaaag
      421 aagtattgct aacgtaaaga taatagggcc actttcaaag aatatagctc tggtaaatat
      481 aaaacctttt ccagacaaag ccatacagga tgaaattttt tctgttttgt cagcagagag
      541 tagcataaac gaagtgtggt ttgccgataa gcctatagag cttgaaattc ttgccaatcc
      601 agggatatat aataatatgg attcaaagta ctgggttttg aacggcaggc ttgcgccgga
      661 gttttcttgt ccggttacgg ctgtatggaa aaaaagcagg aacaataaac tgcatacatt
      721 tgcagttaca ggattcagat acatataagt aaaacaaaat aaatcaaaaa tttatgatat
      781 aatatatttt gtaacctggt tatcaggatt aatatattat ttttaatatt atggtggagc
      841 atgagactaa aatactttat cttagggtat atttttctgt ctgcaacgtt tttgtgggct
      901 tttgaaaaaa taaatgttgc cgttaccccg gcatatgttc cgtatgttct tgaaatcgag
      961 cagggtaaac tttccgggct gtcaattgac ctttggaacg aatgggaaaa gcagaccggt
     1021 atagaggttg agtttgttcc agtcaccctt aaagagggtc ttgcaatgct taaaaacaat
     1081 gaaattgatg ttatggannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngcc
     1201 ggcagtgctt tattatctca acaaatatga atcacttgac ctgtttaaaa tatcagagcc
     1261 gctttatact gaaaaagtaa gaagaggagt gcctcagggg cgccaagact tgctccaggt
     1321 tgtggaggat ggttttgcta agataccgtc caaaaaggtt caggatatcc tgaaaaagtg
     1381 gaaaggtcag acagatcctt caaatataat gagacagatc ctttatatag gaatggttgc
     1441 ttgcggtata atatttgcag tatttttgct ctggtcttac tttcttaaaa gacttgtaga
     1501 cataaaaacc aaagagctga acgaaaaaaa ccgtgaactt acatatgcca ataacgaatt
     1561 agtggctata aataaagagc ttgaacattc tttcgagcag atcgaagagc tttcggaaaa
     1621 gtttgaggat cttataaaca tggcatcggt agcgttcttt gatactatca ataaagatga
     1681 aacttttttt aaaagacttt tggaaacagc tatgcgtata attcccgagt ccgattatgg
     1741 gagcatctct ctcgtagacg gtgatcaatg gacttttgtt gccgctgtcg gacatgatct
     1801 ggaaaagctt aggcttatac cgttaaaaaa atcatatctg gtctttttct gtgaaccggt
     1861 tattgttgaa aagctgagag atcagaatag agagcttcca gacggtatat accaaaaaat
     1921 agaggaagct tctatgcctg ttaggtactc gcttatagca ccacttgtgg ttaacggaga
     1981 gtatcttgga aatatagatc ttgatattga tgaaaattct gaaaaagcat tttcaaaaga
     2041 ttctataaag attctaaaag ccttttccaa tattgccaac gcatttcttg ctttcaaaag
     2101 atactctgat gtaagaagct ccttccagaa ggaagttata acttccatgg taaatattct
     2161 ggaggttcat gacgaatata ctaaaggtca ttccagcaat gttgccgaga tatctctaaa
     2221 catagctaaa aagatgatga tgagtcagga agatatggaa aagatcttct gggcgggact
     2281 tctgcatgac ataggaaaga tactcattcc aactgatata cttaacaaac ccgccaagct
     2341 tacatatcag gagtatgaag aggttaaaaa gcatcctgca tacgggtatg atatcctctc
     2401 aaaatcaaaa aacctcagag agatagccga tattgtcctt taccaccacg aaagatacga
     2461 tgggaatgga tatcccaata aaatcaaagg tgttgatatt ccgctgcttt caagaataat
     2521 atgtgttgct gattcaattg acgcgatgta ttccaaaaga tcttacagaa agccgcttac
     2581 ttgggaacag ataaaccaag agcttaagga caacaccgca agacagtttg atccggatat
     2641 tatagaagtg gtacttaaaa tgagcaagga agaaattttc aggtaggact gcagagcagt
     2701 catcttgaaa gtgtgaaata catagaacgg aggctgatat gaaaaaaagt ttttatgcaa
     2761 gtgcagttat aaatggtctg tgtggcggag cggttcaatt gaaagatgat gcattttatt
     2821 tcatgtgtca gaagcttaca gtacctgaag agtataaaca gttaaggctt gtttacagtg
     2881 atatcagttc tataaacttt aaaagggtct tgtggatact tcctatgact acaatagaat
     2941 taaaaagcgg taagacttac agatttttta tttttaatca aaaaggcttt attaagtcta
     3001 tgaatgaaat gtctaaaaag taaatgttaa taaaacgatc cggatatttt tttagagaat
     3061 ttcagcagaa gagggggaga aaaggatgga ttttttttca gaggctttgg ctgttatgga
     3121 ttctcagatg gggtatgatg tttgtatttc tcttgcaact ctttctgatt ctggtccgag
     3181 tgtgagagtg gttgatgggt actatcatgg cggaagtctt tatgtggttt cacatacggc
     3241 atcgcacaaa atgaaagata ttgccaaaga tccaagggtt gcagggtgtt ataaaattca
     3301 tcagttctgg ggaaaaggtg ttaatatggg taatcccaaa aaaccggaaa atttggagct
     3361 tgccagaact ctcagaaagg tttttttcaa attctatgac aggcatgtga acgaagatga
     3421 tcctgagtgc tgtattctta ggatcgttct ttccagggca gtagttttca gtcaggatgc
     3481 aaagtatata gttgattttc agaacaggtc agcggaaaag atagctatga agcttgacat
     3541 aatagaataa acatcctttc ttaaaaagaa
//
LOCUS       DAOSHR010000204         3568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_24251_length_3568_cov_4.392257, whole genome shotgun sequence.
ACCESSION   DAOSHR010000204 DAOSHR010000000
VERSION     DAOSHR010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 3568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3568
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24251_length_3568_cov_4.392257"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1077)
                     /locus_tag="PLU28_09480"
     CDS             complement(<1..1077)
                     /locus_tag="PLU28_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35328.1"
                     /translation="MCTPSGRNYTSAEYNNARRLAKREFGLKISKGGSSGYLPALEDI
                     IKNTEIVSDSDLGLVEIPLKKVNGTFQRARSTSFAKNFLPLLDEHSEFARKWCDLFEM
                     HQNEGIRDPISVYEYLNWFYVIEGNKRVSVLKYLDAFSITGQVKRLIPKYDPLDSDIR
                     IYYEFLEFYKATKINMIWFSQTGSFKKLLSILFNNYGPQTTEATAGYRAFMNDVYFPF
                     RKIYLALSGQRLAVTTADAFLKFTDRYGFFPQITEKEHKAFIQNIIQLLDEDSYRSQQ
                     NVIVEPPKIILPRISDILNPPKKLKAAFLYLGNKDDYSWNYSHEAARQKVQKTFQERL
                     ETTFYERADTDERVHDLLQQFEKES"
     gene            complement(1086..1817)
                     /locus_tag="PLU28_09485"
     CDS             complement(1086..1817)
                     /locus_tag="PLU28_09485"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOG35329.1"
                     /translation="MLEIICISDEEKNLINKNHKKYDLLISCGDLSPRYMDYVIEEFN
                     PSFALMINGNHDEKYYPAGVKEKSGFSDIFRGIYVLKQGYINLNRYIKKNIGIMGFSG
                     ALSWGAQPFHFQEKNVSKFRKSVDRRKKIGLIQELDFFISHTPPYIQDAFKNMGTYHQ
                     PSYELGSFYEKYFPKIWFYGHIHSSYTSQNLDFKVRKNSGYSFLINACPVKFILYDEE
                     YKKLMDVTSIQKVMFSPLDLSVKIK"
     gene            2038..>3568
                     /locus_tag="PLU28_09490"
     CDS             2038..>3568
                     /locus_tag="PLU28_09490"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOG35330.1"
                     /translation="MKKIAITVILVITAVLSVFPATYGLSVAGGGSYVFQSMGYLDAL
                     MNKGFFPDQIVGTSMGSVVAYYMAGGYYPAQVYNMFSILDFSKFFEMSLPINGGIVNT
                     AGFGDLLYYTTKIDSFDKFMVPVKFGAVNLNKFDSEYINKGDPIRAVRAAVAIEGLFE
                     PLKIGNCYYADIGIRDVNVVYPKSQFTTEKTVSIRFDSPKPDFTGMNFSNIINVLYTS
                     VRMGSYVSNSLYASDKLDYDVSWILAEPAFDLKFFTNGIDLYKTGNDLGLKYLKDNPK
                     FFENNKLTKKTIQTKVIDVYDVYAKVSNESYYRPREFYSTLRMNPGTGRDWHVLDSYI
                     YAEIMNSRFYGGIKWDLFTMKLYPYIRMKQYYLPFESNRLDIQMVMEDYFSTVLRTYI
                     MEGFRRNIYIDASYKFDFVQRKNMFEAAIGYDGMFKTFKNEDGIKAQAAVRTNMQTND
                     YKLELTGVLDLNDKWFKTTLGYIENSDGFFDFTEMTDFGSCSQKVYMRNRLKIGVLDN
                     LN"
BASE COUNT         1073 a          631 c          719 g         1145 t
ORIGIN      
        1 actttctttt tcaaactgct gaagaagatc gtgtaccctt tcgtcagtat ctgcccgttc
       61 ataaaaagtt gtttcaagtc tttcctgaaa ggtcttttgt actttctgcc tggcagcctc
      121 gtgagagtag ttccaactat aatcatcctt atttcccaga tacaaaaaag cagccttgag
      181 ttttttgggt ggattgagta tatcagatat tctgggcagg attattttag gaggttcaac
      241 tattacattc tgttgagagc ggtatgaatc ttcgtcaagt agctgtatga tattctgtat
      301 aaaagcttta tgctcttttt cggttatctg tgggaaaaaa ccgtatctgt cggtaaactt
      361 cagaaaagca tcagctgtag ttacagcaag ccgttgacca gaaagtgcca gataaatctt
      421 tctgaacgga aagtacacat cattcatgaa ggctctgtag ccggcagtag cttctgttgt
      481 ttgaggaccg taattattga agagtataga cagcagcttt ttgaaggaac cggtctgtga
      541 gaaccagatc atatttattt tggtggcttt gtaaaactca aggaactcat agtaaattct
      601 gatatcgctg tcaagaggat cgtactttgg aataagccgt tttacctgtc cggtaatgga
      661 aaatgcatcc agatacttaa gtacgctaac ccttttattt ccttcgatga cataaaacca
      721 gttaagatat tcatagacac ttataggatc acgtattcct tcattttgat gcatttcgaa
      781 aagatcacac catttacgtg cgaactcaga gtgttcatcc agaagaggta aaaaattctt
      841 tgcaaaagaa gtacttcttg ctctctgaaa agttccgttt acttttttaa gaggaatttc
      901 tactaatcca aggtctgaat cagaaacgat ttcggtgttc tttattatat cttcaagtgc
      961 aggaagataa ccgctggaac cacccttgga tattttgagt ccgaactctc tcttggcaag
     1021 tcttcttgcg ttattgtatt cggcgctggt atagtttctt ccggaaggag tgcacatttt
     1081 tcacctcact ttatttttac tgataggtcc aatggactga acatgacctt ttggatggaa
     1141 gttacgtcca taagtttttt atactcctca tcatatagga taaactttac aggacacgca
     1201 tttataagaa aagagtaccc gctgttcttt ctgaccttaa aatcaagatt ttggctggtg
     1261 taagaagaat gaatgtggcc atagaaccat atttttggga agtatttttc ataaaagctt
     1321 ccaagctcgt atgaaggctg atggtaagtt cccatgttct taaaagcatc ctgaatataa
     1381 ggtggagtat gggatataaa gaaatccaac tcttgtatca gaccaatttt ttttcttctg
     1441 tcaacagact tgcggaactt tgaaacattc ttttcctgaa agtgaaatgg ttgagctccc
     1501 catgaaagcg cacctgaaaa tcccattatt cctatgtttt tcttaatata cctgtttagg
     1561 ttgatatagc cctgttttag aacgtagatc cccctgaata tgtcggaaaa accgcttttt
     1621 tcctttactc ctgcgggata gtacttctcg tcgtgatttc cgtttatcat cagggcgaac
     1681 gaaggattga actcctctat aacatagtcc atgtatctgg gcgaaagatc gccgcagctt
     1741 ataagcaggt catacttttt gtgattttta tttataagat ttttttcttc atctgaaata
     1801 caaataatct caagcatgct ttccctcttt tcaaagcttt gtacaatgtt atcataattt
     1861 ttttgtctcg gtcaagataa ggtaatccaa agaacataaa ataatcatta gatgccacaa
     1921 tatacggagg tagagatttg aaatcttttt tcaacaagat catcagttta ttgttcggtt
     1981 ttttgacagg tgcgtatctt ttttttcttg taagcggtat aatgggggga taaacttttg
     2041 aaaaaaatag cgattacagt tattttagta attaccgccg ttctttccgt ttttccggct
     2101 acttatggtc tttcagttgc cggaggaggt tcatatgtat ttcagagtat gggatacctg
     2161 gatgcattga tgaacaaggg tttctttccg gatcagatag ttggaacttc tatggggtca
     2221 gtggttgcat actatatggc tggaggatac tatcctgcac aagtatataa catgttctcc
     2281 attcttgatt tttcaaaatt ttttgaaatg agcctcccga ttaacggagg gatagtaaat
     2341 acagctgggt tcggagatct gctctactat actacaaaaa tagattcttt tgataaattc
     2401 atggttccag ttaagtttgg tgcagttaac ctgaacaagt ttgacagcga gtatataaat
     2461 aaaggcgatc ctataagggc tgtgagagcg gcggttgcca tcgaaggtct ttttgaacct
     2521 ttgaaaatag gaaactgcta ctatgctgat atagggataa gagatgtaaa tgtcgtctat
     2581 cctaaaagtc agtttacaac agaaaaaact gtttcaataa ggtttgacag ccccaagcca
     2641 gattttacag gaatgaattt ttccaatatc attaatgttc tctatacttc ggtcagaatg
     2701 ggaagctatg tctctaacag cctttatgca tcggataagc ttgattacga tgtttcctgg
     2761 atacttgcgg aacccgcttt cgatctaaag ttcttcacaa acggaataga tctgtataag
     2821 accggaaatg accttggatt gaagtatctg aaagataatc cgaaattttt tgaaaataat
     2881 aaacttacaa aaaagacaat tcagaccaaa gtaatcgatg tatacgatgt ttacgcaaaa
     2941 gtttcaaatg aaagctacta taggccaagg gagttttata gtacgctgag gatgaaccct
     3001 ggaaccggca gagactggca tgtccttgac tcatatatct atgcggaaat aatgaacagc
     3061 aggttttacg gcggtataaa atgggatctg tttactatga agctctatcc gtatataaga
     3121 atgaagcagt actatctacc ttttgaatca aacaggctgg atattcagat ggtaatggaa
     3181 gactacttca gtaccgttct cagaacttac atcatggaag gcttccgcag gaatatctat
     3241 atagatgcaa gttataaatt tgatttcgta caaagaaaaa acatgtttga agcagccatc
     3301 ggatacgacg gcatgtttaa gacttttaaa aatgaggatg gtattaaagc acaggcagca
     3361 gtaagaacca atatgcagac caacgactat aagcttgaac ttacaggagt gctggatctt
     3421 aacgataaat ggtttaaaac cactttggga tatattgaaa attcagacgg attctttgat
     3481 tttacagaga tgacggactt cggaagctgc agccagaaag tttatatgag gaacagactt
     3541 aagataggcg tattagataa tctcaatg
//
LOCUS       DAOSHR010000205         3538 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_24517_length_3538_cov_4.467700, whole genome shotgun sequence.
ACCESSION   DAOSHR010000205 DAOSHR010000000
VERSION     DAOSHR010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 3538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3538
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24517_length_3538_cov_4.467700"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /locus_tag="PLU28_09495"
     CDS             complement(<1..730)
                     /locus_tag="PLU28_09495"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG35331.1"
                     /translation="MNEAKDPILTLNNYSLKIEDKLILSDINLKIYPGEIILFYGPRN
                     SGKSSLLRTFVHLNEELYNHVKGGGDLLYNGKDMRKMDRKFLRSQITYCDTSFVENLN
                     FLTLNELLKISIDLKIKDLTKEHFETLDKLGISHIFANNESLKYYKNLSNWSIGEKIS
                     LITFLALARNPRIFIFDSILDHLDDYLLRQVKDLFASIKENITLMVSTRNIALFSDMA
                     EKIALIDKGKLEYYGSITKFMLNFP"
     gene            complement(742..903)
                     /locus_tag="PLU28_09500"
     CDS             complement(742..903)
                     /locus_tag="PLU28_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35332.1"
                     /translation="MKNNICVPMLNLKLASQYEFIKFFKQAKNPKELEKIARLRRGTV
                     KDNLNSEKK"
     gene            complement(1086..2345)
                     /locus_tag="PLU28_09505"
     CDS             complement(1086..2345)
                     /locus_tag="PLU28_09505"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HOG35333.1"
                     /translation="MTIFLSKIYFPIIVLILVYVLIAIRQIGKFRIQPWQSMMLGAVL
                     VLVTGQIKPANAFKAVNFDVLIFLFGMFVIGEAFAQSGYIYHLSYKAFNKAKTINQLL
                     WLIIISMGLLSPFFINDTMVIVSTPLIIYLAKKQNIPAKPLILAMMFAVTTGSVFSPI
                     GNPQNLLIAMNGNFSNPFIVFFKYLLIPTLINLFIIFFFIKLYYKKNLSNVKELIHSE
                     EKPTDGNLYKISKFSFYLTFALIGLKMLSFLFGLGDSFKLTYISLIASSPILLFSSKR
                     FSVLKKIDWYTLIFFSSMFILMESVWETGIFQKMVNGYNLSSIPVILVLSILTSQLIS
                     NVPFVSLYMPLILGLNATAKELTALAAGSTIAGNLLILGAASNIIAMQSAERNGEVLT
                     FSDFAKIGIPLTAINAFVYWFFFNIIP"
     gene            2470..>3538
                     /locus_tag="PLU28_09510"
     CDS             2470..>3538
                     /locus_tag="PLU28_09510"
                     /inference="COORDINATES: protein motif:HMM:NF015563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35334.1"
                     /translation="MAEENFGIKIGKKAFLSSFFILLGLIVFAGILTLAVPTGSYERQ
                     IVNGSEVFNWESFSFTQEDKLPVYRWFTAPLEVLGTSDGVKALALIILLLIIGGSFAV
                     IDKANVLKSIVSSIVKKFRNKRYFLIAIITFIFMGFGSVLGIFEEAVPLVPIIIALSY
                     LMGWDALVGLGMSLLAITFGFSSAISNPFTIGLAQKLTDLPAFSGTWYRLIIFAVIYT
                     VLVFFLVRYAKKIDKDAKKSLVYEEDLVERKKYSSADGFINENFKDKKTVNAVRCFLI
                     MIFLMLFVIVLGIFIPQVSDMALILMALLYLIAGICTGFLAGMGVLNTFKVFAKGMAG
                     ISASAVLILMGISVKHIIVSGN"
BASE COUNT         1106 a          533 c          664 g         1235 t
ORIGIN      
        1 taggaaaatt tagcatgaac ttagtgatgc ttccgtaata ttcaagtttt cctttgtcta
       61 ttagagcaat tttttctgcc atatctgaaa acaaagcaat atttcttgtt gaaaccatta
      121 aagtaatgtt ttcttttata cttgcaaaaa gatcttttac ttgtctaaga aggtagtcat
      181 ccagatgatc caatattgaa tcaaaaataa atattcttgg atttcttgcc aatgccaaaa
      241 aagttataag ggaaattttt tctccgatag accaattgga taggttttta tagtatttaa
      301 gcgattcatt gttagcaaat atgtgagaga tgcctaattt gtcaagcgtt tcaaagtgtt
      361 ccttagtcaa gtcttttatc tttagatcaa tggaaatctt aagaagttcg tttaaagtaa
      421 gaaaattaag attttcaaca aaagaggtat cacagtatgt aatttgtgat ctaagaaact
      481 ttctgtccat ctttctcata tctttaccgt tgtatagtag atcaccgccg ccttttacat
      541 ggttgtacag ttcttcgtta aggtgaacaa atgtccttaa taaagaagat tttccggaat
      601 tacgaggtcc ataaaaaagg atgatctctc ccggataaat tttcagattt atatcactca
      661 gtattaactt gtcctctatt tttaaggagt agttattgag ggtaagtata ggatctttgg
      721 cttcattcaa ggaaaccact cttacttttt ttctgaattt agattgtctt tcacagtacc
      781 tctgcggagt ctagctattt tctcaagttc tttggggttt ttggcttgtt tgaaaaactt
      841 tatgaactcg tactgcgagg ccaactttaa gttcaacata ggaacgcata tattgttttt
      901 catcttaccc ctcctccaaa tttgaactac aaaaatatga tacacaaaaa actatacagg
      961 aataagaagg ttctttaagg aagttttaag aatagaaata caatgatgtg ctaaaaagag
     1021 ttttaataat aatatgtgta taatgcatta aataaaaaaa ccattttagt tttaaaaaga
     1081 ttatattatg gtattatatt gaaaaaaaac cagtatacaa aggcatttat ggcagtcagc
     1141 ggaatgccaa tttttgcaaa atcagaaaaa gtgagcactt ctccgtttct ttcagcagac
     1201 tgcattgcta taatattgct tgctgctcca aggattaaaa gatttccggc tattgtactt
     1261 cctgccgcaa gtgctgtaag ctctttggca gtagcgttaa gtcctagaat cagaggcata
     1321 tagaggctta caaaaggaac gtttgatata agctggcttg taagtatgct taacacaagt
     1381 attacaggta ttgatgaaag attgtagccg tttaccattt tttgaaatat tccggtttcc
     1441 catacacttt ccattagaat aaacattgaa gaaaagaaga taagtgtgta ccagtctatt
     1501 tttttcagaa cgctgaatcg tttggagctg aacagtaata tcggcgagga cgcaatcagc
     1561 gaaatatatg tcagtttaaa gctatctcca agtccaaaga ggaaggagag catttttaat
     1621 cctatgagtg caaaagtcag gtaaaaagag aatttagata ttttatatag gttcccgtct
     1681 gttggttttt cttcagagtg tatcaattct ttgacatttg aaagattctt tttgtaataa
     1741 agttttatga agaaaaatat aataaaaaga tttataagag ttggtatcaa cagatattta
     1801 aaaaaaacta taaaagggtt tgaaaagttt ccgttcatag cgatgagcaa gttctgggga
     1861 tttccgatcg gactgaatac gctgcctgtg gttactgcaa acatcatagc cagaataaga
     1921 ggttttgccg gaatattttg tttttttgca agatatatta tcagaggggt tgacactatg
     1981 accatggtgt cgtttataaa aaacggagaa agcaagccca tgcttatgat tatgagccac
     2041 aagagctggt ttattgtttt ggctttatta aaggccttgt atgaaagatg gtatatatat
     2101 ccgctttgtg caaaagcttc cccaataaca aacataccga aaagaaagat cagaacatca
     2161 aaattgacgg ctttaaaagc atttgctggt tttatctgtc ctgtaacaag aacaagaact
     2221 gctccgagca tcatgctttg ccatggctgt atccggaact tccctatttg tcttattgct
     2281 atcaaaacat agactaagat caatactatt attggaaaat atatttttga taaaaatatt
     2341 gtcattttct gccatctccg ttatataatt tattcagtag atactatcag aaatacttta
     2401 agggttggtt atttatttaa taaatatgga atataaataa gtttaataaa tattaccggg
     2461 ggttaaatca tggcagaaga gaattttggt ataaagattg ggaaaaaagc ttttctttca
     2521 tcttttttta ttttactggg actgatagta tttgccggta ttctgacttt agcagtgcct
     2581 accggcagct atgaacgcca aattgttaat ggaagtgaag tatttaactg ggagtctttt
     2641 tcttttacgc aggaagataa gcttcctgtt tatagatggt ttacagctcc tttggaggtg
     2701 ctgggaactt ctgatggagt aaaagctctg gcgcttataa tacttcttct gatcataggt
     2761 ggatcttttg cagtaataga taaggcaaat gttttaaaat caatagtatc gtctatagta
     2821 aaaaaatttc ggaataaaag atactttttg atagctataa taacctttat ttttatgggt
     2881 ttcggatctg ttcttggaat ttttgaagag gccgttcctt tggttccgat aataattgct
     2941 ttatcgtatc ttatgggttg ggacgctctt gtggggctcg gaatgagtct tcttgccata
     3001 acctttggtt tttcttctgc catatctaat ccttttacaa taggtctggc tcaaaaactt
     3061 accgatttgc ctgctttttc agggacttgg taccggctga ttatatttgc tgtaatatat
     3121 acggttctgg ttttctttct tgtaaggtat gcaaaaaaaa tagacaaaga cgccaaaaaa
     3181 tctttggtat atgaagaaga tctcgttgaa agaaaaaaat acagttctgc cgatgggttc
     3241 ataaatgaaa attttaaaga taaaaaaaca gttaatgccg taaggtgttt tctgattatg
     3301 atatttctta tgctttttgt tattgtactt ggaattttta ttcctcaagt ttctgatatg
     3361 gcgcttattc ttatggcgct tttatatctt atagctggta tatgtacagg ttttcttgca
     3421 ggtatgggcg tgcttaacac ttttaaggtt tttgccaaag gcatggcggg catatctgct
     3481 tctgctgttc ttattcttat gggtataagc gtaaagcata taattgtaag cggcaaca
//
LOCUS       DAOSHR010000206         3494 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_24966_length_3494_cov_3.846758, whole genome shotgun sequence.
ACCESSION   DAOSHR010000206 DAOSHR010000000
VERSION     DAOSHR010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 3494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3494
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24966_length_3494_cov_3.846758"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..150)
                     /locus_tag="PLU28_09515"
     CDS             complement(<1..150)
                     /locus_tag="PLU28_09515"
                     /inference="COORDINATES: protein motif:HMM:NF017941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HOG35335.1"
                     /translation="MKTKRIVYSGLVAALYVVLCTVFQPISFRAVQFRVAEALTVLPF
                     LDPVFI"
     gene            317..1810
                     /locus_tag="PLU28_09520"
     CDS             317..1810
                     /locus_tag="PLU28_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35336.1"
                     /translation="MKKLFFISIVFILSLSGFSKVYTGSDGLIFDFYPSYIQENSDIK
                     IKSVSVLAGFNQYDIAEGRMIELQGFWRYSKKLWDGPVWFYYLVETAEGKTFEIIDPD
                     VFSLSDRNYFFLYEDKINRAHNIFGYDYMYRLNSDQGVTLQHVLGGQSFSDWYFQARL
                     KLNSAFFQAGSQLSFGIQTDLKEFDPYIIFSGYAALGNRAFNAGYKNTAYTGSGDYLK
                     YFTPLNSIRDRYFMLTKDFAFSDKVKKQMLLSDTESYSNIPAVFLNLDFPIFTGGARM
                     YDLEPYRKISLSNKYGYDLFVVFKNEGFFLGGQWLSENIKDLNYFSPISPPQYNGDFV
                     LLTAGYNENAVNAVFYDYTQFYSVAVKTLFKFNSSNIYDYFVYDHMHKLASLYLTHNW
                     QQELYAMYNFSGLYPSFFIDFMISQPLGEIMLKALGHFTYDELLVNRFGTNTAFYYKL
                     GMQYSSGTITAEVSGGNTSFFSVWDGWGIKEEDPSQMSINLSFKHSI"
     gene            1825..>3494
                     /locus_tag="PLU28_09525"
     CDS             1825..>3494
                     /locus_tag="PLU28_09525"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG35337.1"
                     /translation="MKEPKYAIVYLTLKADYAKLNNLPVRLPLLAEDFQKSRITKKVE
                     LEYIINGLAAQYETDRNDEYYRSYLVFYYYEGLKAAMSSGDFLKARLYLEKAEKIHKD
                     YRFFFYSGLISKNEKDYVRAELELKECISLKKEFVPGHFELADLMHITGNDDEAVEAC
                     LTINELEPSFIPAYLKAADIYAENGITDKAFEYLKKCLEKDSRFAPAYLRIGVLLNQM
                     QQFAQALAAFEKGCAYDPQNYEFYYNMAFSLGRLGRHLQAVQVLEKACSINPEDFVYH
                     ELAIEYKNLGMFLKAFEAEKKAFDIASQENKDLITLSLMKMAVFVEDYDTIEQIYKTS
                     VDKEIKTGVMTFKMFFDLGQGEVHKAAVQLSKLTAEGLFPVLEQRIYQVEKIVQKLED
                     RCDEGLAQSLLESIDEDGEINCQQLYEKLRDGKYSGEYIGSLIENSNTAKERPSGLEL
                     VITGLFLSGMNYGLAERVSATLARLLWKDGNGIAFARFILRLYQAKVFGEYTDLDTFV
                     QDVILEITDLSYKIGSMLTQYNEKLMDFDTLVHTKIKKFEDAFMVLISA"
BASE COUNT         1093 a          639 c          753 g         1009 t
ORIGIN      
        1 aatgaatacc ggatcaagaa acggcaggac tgtaagggct tctgcaactc tgaactgaac
       61 agccctaaag cttatcggct gaaaaacggt gcacaaaaca acatacagag ctgcaaccag
      121 ccctgagtag acgattctct tagttttcat ataaaaacct ccagtttttt tagagcgggt
      181 gctgcgaccg cttttatctg cattgagaat taaaacatat tttggttttg tttttattgt
      241 ggagttgtaa ataatgatgt ataataaaaa tgaatactaa atccataaaa aaagagaaaa
      301 ccgggggtga aatccgatga aaaaattatt tttcataagt atcgttttta ttttgtcttt
      361 gtccggattt tcaaaggtat acaccggcag cgacggactg atatttgatt tctacccttc
      421 atatattcag gagaattcag atataaagat aaaaagcgta agtgttttgg ctggatttaa
      481 ccagtatgac atagccgaag gacgcatgat cgaacttcaa ggcttttgga ggtactccaa
      541 aaagctttgg gatggaccgg tctggtttta ctatttagtt gaaaccgctg aggggaaaac
      601 ttttgaaata atagatccgg atgttttttc actgtcagac aggaattatt tctttctgta
      661 cgaggacaaa ataaacaggg cacataatat ctttggatat gattatatgt atcggttgaa
      721 ctccgatcag ggcgttactt tacagcatgt tctaggaggc cagagttttt cagattggta
      781 ctttcaggcc aggctgaaac tgaattcagc tttttttcag gcaggatctc agcttagctt
      841 tggtatacag accgatctta aggagtttga tccgtatatt attttttcag gttatgctgc
      901 tttaggtaac agagcattta atgcaggcta taaaaacaca gcctataccg gctctggcga
      961 ttatctgaaa tattttactc cgcttaacag catacgggac agatacttta tgctgacaaa
     1021 ggattttgca ttcagtgata aagttaagaa acagatgctt ctttcggata cagaaagcta
     1081 ttctaatata ccagccgtat ttcttaatct tgattttcct atttttaccg gcggtgcgag
     1141 gatgtacgac cttgaaccct acagaaaaat ctctctttca aacaagtatg gatacgacct
     1201 gtttgttgtg tttaagaacg aaggcttttt tttgggtggc cagtggctta gtgagaatat
     1261 aaaagatctc aactatttca gccctataag tcctccgcag tataacggtg attttgtact
     1321 gcttactgca ggctataacg aaaatgccgt gaacgctgtc ttttatgatt acacacagtt
     1381 ttattccgtt gccgtaaaaa cccttttcaa attcaacagt tcaaatatct acgactattt
     1441 tgtatacgac catatgcata aacttgccag tctgtatctg acgcacaact ggcagcagga
     1501 gctttatgcc atgtataatt tttccggtct ttatccatcg ttttttattg attttatgat
     1561 cagccagcct ttgggtgaaa taatgcttaa agctctgggg cattttacgt acgatgagct
     1621 tcttgtgaat agattcggaa ctaatactgc tttttactat aaacttggta tgcagtacag
     1681 tagcggaacg ataacggctg aagtaagcgg cgggaacaca tcgttcttct ccgtatggga
     1741 cggttggggg ataaaagagg aagatccttc tcaaatgagc attaacttaa gtttcaaaca
     1801 ttctatttaa aggacggagg aaaagtgaaa gaacctaaat atgcaatagt gtatctaact
     1861 ctgaaggctg attatgctaa actcaacaac cttccggtac ggcttcccct tctggccgag
     1921 gacttccaga agtcaagaat aaccaaaaaa gtggagcttg aatatataat aaacgggctt
     1981 gccgcccagt atgaaacaga caggaacgat gaatactatc gctcctatct tgttttttac
     2041 tattatgagg gtttgaaagc agcgatgagc tcgggcgact ttcttaaggc aagactgtac
     2101 cttgaaaaag ctgaaaaaat acataaggac tatagatttt tcttttattc cggtcttatt
     2161 agcaaaaatg aaaaagatta tgtaagagct gagctggagc ttaaagagtg catatcccta
     2221 aagaaagagt ttgttcccgg acatttcgag cttgccgatc ttatgcacat aaccggcaac
     2281 gatgatgaag cagttgaagc atgtctgact ataaatgaac tggaaccttc gtttataccg
     2341 gcttacctta aagcagcaga tatttatgct gaaaatggca taacggacaa agcctttgaa
     2401 taccttaaaa aatgtcttga aaaggattct aggtttgctc cggcatatct gagaataggt
     2461 gtgcttttaa accagatgca gcagtttgca caggcactgg ccgcctttga aaaggggtgc
     2521 gcatatgatc ctcaaaacta tgagttctat tataacatgg ccttcagtct tgggcggcta
     2581 ggaaggcatt tacaggcggt acaggtgctt gaaaaggctt gcagtattaa tcctgaggac
     2641 tttgtatatc acgagctggc cattgaatat aaaaatcttg gaatgtttct gaaagctttt
     2701 gaagccgaaa aaaaagcctt cgatatagct tcacaggaaa acaaagatct tataacactc
     2761 tctttgatga aaatggcggt ttttgttgaa gattacgata cgatagaaca gatatacaaa
     2821 acatctgtcg ataaagagat aaaaacaggg gtaatgacat tcaaaatgtt ttttgacctt
     2881 ggtcagggtg aggtacacaa ggctgccgtc cagctcagca aattaacagc cgaaggactc
     2941 tttccggtac tggaacaaag aatataccag gtagaaaaaa tagttcagaa gcttgaggac
     3001 aggtgcgacg aaggccttgc ccagagtctc cttgagagta tcgatgagga tggagaaata
     3061 aactgtcaac agctttatga aaagctcaga gacggcaaat actccggcga gtatatagga
     3121 agccttatag aaaactcaaa tactgccaaa gaaagacctt cggggcttga gcttgtcata
     3181 accgggcttt tcctgagcgg tatgaactac ggactggccg aaagggtaag cgcaactctt
     3241 gcacggttgc tctggaaaga cggaaacggt atagcctttg caagattcat actcagactc
     3301 taccaagcaa aagtattcgg agagtataca gatcttgata cttttgtgca ggatgtaatt
     3361 cttgagataa cagatctcag ctataaaata ggaagcatgc ttactcagta caatgaaaaa
     3421 ttaatggact ttgacacact ggttcataca aagataaaaa aatttgaaga tgcttttatg
     3481 gtgctcatct ccgc
//
LOCUS       DAOSHR010000207         3476 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_25143_length_3476_cov_4.305466, whole genome shotgun sequence.
ACCESSION   DAOSHR010000207 DAOSHR010000000
VERSION     DAOSHR010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25143_length_3476_cov_4.305466"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            339..566
                     /locus_tag="PLU28_09530"
     CDS             339..566
                     /locus_tag="PLU28_09530"
                     /inference="COORDINATES: protein motif:HMM:NF021452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2164 domain-containing protein"
                     /protein_id="HOG35338.1"
                     /translation="MGSIEIDQQKKKVLIEKIKVFYSKEMEEEIGEFKAKKLMDFFMN
                     ELAVQIYNMAVEDAKEYMSQRIEDLLSIQKY"
     gene            complement(667..1419)
                     /locus_tag="PLU28_09535"
     CDS             complement(667..1419)
                     /locus_tag="PLU28_09535"
                     /inference="COORDINATES: protein motif:HMM:NF019359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOG35339.1"
                     /translation="MKYYTIKQIADMAGITVRTLHHYDHVKLFCPDGKGANGYRIYTS
                     TQLQKLRQILFFKELGFTLAQIKEILDSENFDRRKALQIHKELLLNKRQRLDELINNV
                     DRNIKALEGGLEMNEEEMFSPFEDKKIREYTQQAREKYSNSLVEQSIEKVSAYSKKEW
                     EDIMEYGDSLMKEMKGLMNMDPADKTVQNVISKHYQMINDNFYDCSIEIYSGLADLYI
                     SDERFTAFFEKYAQGLAKYMHDAIKIFCSEKR"
     gene            complement(1518..>3476)
                     /locus_tag="PLU28_09540"
     CDS             complement(1518..>3476)
                     /locus_tag="PLU28_09540"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG35340.1"
                     /translation="LLDTSFNYSTFIFSLSGILIFIALFKLKLFGIQPIAKNILIENL
                     SDGVLVLDRENTVIDMNIITKKIFGLNDSCIGKKMGSVFYDFPLFLESIGINVALSDE
                     MPIGEKFYDIRITPINDFKNIFLGRLIVLRDITERKSFEKEIVKAKEEAEYANEAKSR
                     FLANMSHEIRTPLNGIIGYLSLLSTTELSVSQKEFVQNARLSSNILMEIINNILDISK
                     IEAGKIELEKTDFSLDELIDEIQVMFTPKVEEKGLLFSIKKEFQPKNTFIGDTLRIKQ
                     ILINLINNAVKFTSNGSIVLYVHVFEPEDGKYRLHFEVTDTGIGMTHEQQEKIFSPFT
                     QADNSTTRKYGGTGLGLSIAKSLVELMHGKIWVHSTPNEGSVFMFDILLAASEKTGLH
                     STSKKNYMNNLYDFKHTVKILLAEDNTINQGLMKNIFELSGLQYSIVGDGLSAVEKCK
                     AESFDLIFMDIQMPIMDGCQASQKIRECEKMKGSGPVPIIALTASASSKETKSFVNYG
                     ISDFLTKPFELPDFYMILFKYIKDAQYELRPLQKNEKIHSNETEKAIDYIAENIVFDR
                     AEAEKLFQNYINEAETIIENMRECLKKNDYETLDKLAHKLKGSSGFFKLDAIVTMCLD
                     LREYSKQKNAEQADFILKNLTGKILSIK"
BASE COUNT          979 a          627 c          670 g         1200 t
ORIGIN      
        1 actatagaaa gcaccatgct tattacactg agcagagcag aagttataag cttgtaccta
       61 tgctttcccg cctgatgcag cagaaatgaa aaatcacttt cctttttgaa agtatgcttc
      121 attaaaatgc actcctttac cgttttgagc ggcgagcagt attaagtatt ttatgtatac
      181 tagcctacat tatatcaaag aattataaag aagtcaagct ttgaaaaaga taactataaa
      241 agacatctta gtagtttaaa ctactaagat gtcttttata gaagattata tagctgtgat
      301 ataattttcc catgtacata aaaccggagg tgacagccat gggtagtatt gaaatagacc
      361 agcaaaaaaa gaaagttctg attgaaaaaa taaaagtttt ttattccaaa gaaatggaag
      421 aagaaatagg cgagtttaag gcaaagaagc ttatggattt ttttatgaac gaacttgccg
      481 tccagatcta caatatggca gtcgaagatg ccaaagagta catgtcccaa aggatagaag
      541 atttactcag cattcaaaaa tactgaatga gcattcaaaa gtactgaatg agtattcaaa
      601 aatatttaaa aaccaactac cgggttattt tccggtagtt ggtttaaact taacacggta
      661 tgggtattat cgtttttcag agcaaaaaat ttttattgca tcgtgcatat acttggcaag
      721 accctgagca tatttttcaa aaaaggcggt gaatctttcg tcggagatgt aaagatccgc
      781 aaggccgctg tatatttcga tggaacagtc ataaaagtta tcgtttatca tctggtagtg
      841 ttttgagata acattctgta ccgttttatc tgcaggatcc atgttcataa gccctttcat
      901 ttctttcata agtgaatcgc catattccat gatgtcctcc cattcttttt tggagtatgc
      961 tgaaactttt tcaatacttt gctcaacaag agagttagag tacttttctc ttgcttgttg
     1021 agtgtactct cttatttttt tatcttcaaa tgggctgaac atctcttctt cgttcatctc
     1081 taagcctcct tcaagcgctt ttatgttcct atctacgtta tttataagct catcaagcct
     1141 ctgccttttg tttaagagaa gctctttgtg tatctggagt gccttacggc ggtcaaagtt
     1201 ttcactgtcg agtatctctt ttatctgagc aagagtgaag cccagttctt taaaaaatag
     1261 tatctgccgg agtttttgca gttgagttga agtatatatt ctgtatccgt ttgctccttt
     1321 tccgtctggg caaaaaagtt tcacatggtc ataatgatgg agggttctta cggttattcc
     1381 tgccatgtcg gcgatctgct ttatggtata gtacttcatc cttcacctca gttttattat
     1441 atactattac gtaacgtcat agtcaagtaa aaaaatctgc tatccaaagt tagaatagca
     1501 gataaattta gagtttttta tttgatggaa agaattttgc cggtcaggtt tttaagtatg
     1561 aagtctgcct gttcggcatt cttctgtttt gaatactctc taagatcaag gcacatagta
     1621 acaatggcgt ctagtttgaa aaaaccactg cttcccttaa gcttgtgagc aagtttatca
     1681 agggtctcat agtcattttt ctttaagcat tcacgcatat tttcaattat ggtttcagct
     1741 tcatttatgt agttctggaa aagtttttcg gcttcagctc tgtcaaaaac aatattttca
     1801 gctatatagt caatggcttt ttcagtttca tttgaatgaa tcttttcgtt tttctgaagt
     1861 ggacgcagtt catactgggc atcttttatg tacttgaaaa gtatcatgta aaaatccgga
     1921 agttcaaagg gtttagttaa aaagtccgat attccgtagt ttacaaagct tttagtctcc
     1981 tttgagctgg cgcttgcggt cagggcgatt ataggaacag gtccggaacc tttcattttt
     2041 tcgcattcgc ggattttctg cgaagcctgg cagccgtcca ttataggcat ttgaatatcc
     2101 ataaatataa ggtcaaagct ttctgccttg cacttttcca cagccgaaag tccgtctccg
     2161 actattgagt attggaggcc tgaaagctca aatatgtttt tcatcagccc ttggtttatg
     2221 gtgttgtctt ctgcaagcag aattttaacg gtatgcttga agtcataaag attattcatg
     2281 taattctttt tagaagttga atgtagaccg gtcttttcag atgcagcaag taaaatatcg
     2341 aacataaaca ccgagccctc gtttggggtg ctgtgaaccc agatttttcc atgcatcagt
     2401 tcaacaaggc ttttggctat actaaggcca agaccggttc cgccgtactt cctggttgtt
     2461 gagttatctg cctgggtaaa agggctgaag attttttcct gctgttcatg tgtcattcct
     2521 attcctgtat cggtaacttc aaagtgtagc ctgtactttc cgtcctctgg ctcaaagaca
     2581 tggacatata agacgatact tccgtttgag gtaaacttta cagcgttgtt tataaggttt
     2641 ataagaatct gttttattct cagggtgtct ccaataaagg tgtttttggg ttgaaattct
     2701 ttttttatgc tgaaaagcag tcctttttct tcaacctttg gagtgaacat gacctgaatt
     2761 tcgtcaataa gctcatcaag gctgaaatcg gttttttcta gttctatctt tccggcttct
     2821 atcttggata tatcaaggat gttgtttata atttccatga gaatattgga ggaaagcctg
     2881 gcattttgca caaactcttt ctgagagaca gagagctccg tggtggaaag aagactcagg
     2941 tatcctatga taccgttaag aggtgttctt atctcatggc tcatatttgc aagaaatctg
     3001 cttttggctt cgttggcgta ctctgcttct tccttggctt ttactatttc tttttcaaag
     3061 ctttttcttt cagttatatc tctaagtaca ataagtctgc caagaaatat gtttttaaag
     3121 tcatttatgg gagtaatccg tatgtcgtaa aatttttcac cgattggcat ctcatcgcta
     3181 agggctacgt ttatccctat agactcaaga aagagcggga aatcgtaaaa tacacttccc
     3241 atttttttgc ctatacagct gtcgtttaat ccgaagattt ttttggttat tatattcatg
     3301 tctataacgg tgttctccct atccaaaaca agaactccgt cggaaaggtt ttcaatgaga
     3361 atattttttg cgataggctg tatcccgaac agcttgagtt taaaaagagc tatgaaaata
     3421 agtattccgg aaagtgaaaa gataaacgtg ctgtagttaa aagaggtatc aagcag
//
LOCUS       DAOSHR010000208         3447 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_25465_length_3447_cov_5.545991, whole genome shotgun sequence.
ACCESSION   DAOSHR010000208 DAOSHR010000000
VERSION     DAOSHR010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 3447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3447
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25465_length_3447_cov_5.545991"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1538)
                     /locus_tag="PLU28_09545"
     CDS             complement(<1..1538)
                     /locus_tag="PLU28_09545"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOG35341.1"
                     /translation="MKKTLAVFAVLLSVVFMFGAITNPDTIVKATIGGPDTFDVHQAY
                     DNASGEVLSNVYEPLYVFKGSSLSEFEPRLCTDIPTVANGGIKDGGKTYVFKIRKGVK
                     FHEGGDLSPEDVEYTIERGIIASPTGGPMGMFTQYMFGNPDFKDLVAKYVGVAEYKEM
                     FNDAAFQDLKPEYTEKLVKFYKEVVDPAVEVKGDEVYVHLQVPFGPFLTIIGGEAYWG
                     SIIDKEWSISKGLWDGKAEGWWKYHDVQKEKSPIYNVCNGTGPFKMLEWNTTTKQVTL
                     ERFDGYWRAPAKIKTVIIKGIDEWSTRKAMLEKGDADIITVDATYIDQIRNREKEGIK
                     VIDNIPELGVRSISFCWSVDPKSKYIGSGKLDGEGVPPDFFTDENVRQAFSYSFDYVA
                     YIKDTVNGLGITIPTLIPQGMLGFNPNLPTYKLDVAKATQYFKRAFKGQLWLKGFKMQ
                     ITYNTGNVVRQKALEQLRDNLLKINPKFKVEVVGIQWASLLDARKRSELPLFMMGWGA
                     DFPDP"
     gene            1777..1851
                     /locus_tag="PLU28_09550"
     tRNA            1777..1851
                     /locus_tag="PLU28_09550"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1809..1811,aa:Val,seq:gac)
     gene            complement(1891..2574)
                     /locus_tag="PLU28_09555"
     CDS             complement(1891..2574)
                     /locus_tag="PLU28_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35342.1"
                     /translation="MYYHTLKTLYSFLGKDLTKNENIRKIKEEIFSKLQTKTDFLQII
                     SFLEETDDYEVIDCLLDMLAVVIFSKINIVYDHKTLKYGTESIGLKITISEIKEFIDI
                     LDYSDIETQNIFYLFSNDLQKRITGFKNILKREKKFAWREDEIKIYIKNLKPLSMDFL
                     KLLTVYGEVDSDFIVSQLKLKNKKSVSALVSAITRNAPKDKEKFIFHEGDKITMNKEY
                     RSFFATLIK"
     gene            2663..>3447
                     /locus_tag="PLU28_09560"
     CDS             2663..>3447
                     /locus_tag="PLU28_09560"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOG35343.1"
                     /translation="MIKQVTVDEKNYYKRLDKFLRNNFSELKLGTIFMLIRKGNVKIN
                     GKKAKQNETELFIGDDVQIFINDQLEDISRPEVPQFKPRYMDLKVLYEDEYVIAVDKP
                     PKVSMHPGRSEEMVTLIEALIFRASQEYEPHLVHRLDKHTSGVVVVAKTKQIARELTE
                     IIMNRETDKYYIALLSKNLKNAKGTLNSIVDEKDAALEYTVENVYKNLMGSFPLVNVH
                     LLTGRKHQIRVQFSQISSPVLGDDIYGDKDYNRQAKNLFGLKR"
BASE COUNT         1083 a          640 c          587 g         1137 t
ORIGIN      
        1 ggatctggga agtcagcacc ccaacccatc ataaagagag gaagttctga tctctttctg
       61 gcatcaagga gtgaagccca ctgtatacca acaacttcaa ccttgaactt aggattgatc
      121 ttaaggagat tatccctgag ctgttcaaga gctttctgtc ttactacgtt tcctgtattg
      181 tatgtaatct gcattttgaa acccttaagc caaagctgcc ctttgaatgc tcttttaaag
      241 tactgtgttg cctttgccac atcaagtttg taagtcggga ggttcggatt gaatccaagc
      301 attccctgag gaataagagt aggaatggtt atacccaaac cgttaactgt atcctttatg
      361 taagcaacat aatcaaagct gtaagaaaaa gcctgtctta cattttcgtc agtgaagaaa
      421 tctggtggta cgccttctcc gtcaagctta ccggaaccaa tgtactttga tttcggatcg
      481 actgaccagc agaaggaaat agatcttacg ccaagctcag gaatattatc aataactttt
      541 attccttcct tttctctgtt tcttatctga tcaatgtatg ttgcatcaac agtaattata
      601 tctgcatcgc ctttctcaag cattgctttt cttgttgacc attcgtctat accttttatt
      661 ataactgtct ttatctttgc aggagctctc cagtaaccgt caaatctttc aagtgtaact
      721 tgcttagtag tggtattcca ttcaagcatt ttgaaaggac cggtaccgtt acaaacattg
      781 tatataggtg atttttcttt ctgcacgtcg tgatacttcc accaaccttc tgctttcccg
      841 tcccaaagac ctttggatat ggaccattct ttgtcaatta ttgatcccca ataggcttct
      901 cctccgatta ttgtaaggaa aggtccgaat ggaacctgaa ggtgtacata tacttcgtcg
      961 ccttttactt caacagcagg atctacaacc tctttgtaaa acttaacgag cttttcggtg
     1021 tattcaggct ttaaatcctg aaaagctgca tcattgaaca tttctttata ctcagcaact
     1081 ccaacatact tggcaaccaa atctttgaaa tctgggtttc caaacatata ctgagtaaac
     1141 attcccatag gaccaccagt aggagatgct atgattcctc tttctattgt gtattcaaca
     1201 tcttcaggag aaagatctcc accttcgtgg aacttaacgc cctttcttat cttaaataca
     1261 tatgttttgc ctccgtcttt gattccaccg tttgcaactg taggaatatc agtacagagt
     1321 ctgggttcga actcacttaa gcttgaccct ttgaaaacgt aaagaggttc gtaaacgttt
     1381 gataatactt cacccgaagc attatcgtaa gcctggtgca catcgaaagt atccggaccg
     1441 ccaatagttg ctttaactat tgtatcagga ttggtaatag caccaaacat gaaaactact
     1501 gacaacaata ccgcaaatac cgctaaagtt ttcttcattc aactttcccc ccttatatca
     1561 gtctttgtgt ttacattccc taaaatattt catcatgaat attttaccat aactcaaatt
     1621 taattgcaac agttaaatca acaaagttga aaatatgaca tgaaagtttt cgggaagatt
     1681 gacaattgta ataagataca aatttttaat agtatgtaat aaaaaagtat attgacttga
     1741 ctggaaaaat atgatatact tttcatcgga acttaagggt gtatagctca gtgggagagc
     1801 gcttccctga cgcggaagag gccgctggtt caatcccagc tgcacccacc aacgaaagac
     1861 cgggatagta tcccggtctt ttttatattt ttattttata agagttgcga aaaaactcct
     1921 gtattccttg ttcatagtta ttttatctcc ttcgtgaaag atgaactttt ctttatcctt
     1981 gggagcattt cttgttatgg cacttaccag tgcagataca gattttttat ttttaagctt
     2041 taattgtgaa actataaaat ctgaatcaac ttcgccataa acagtaagaa gtttaagaaa
     2101 atccatggaa agcggcttaa gatttttaat ataaattttt atctcatctt cgcgccatgc
     2161 aaattttttt tctcttttca gaatattttt aaaacctgtt attctttttt gaagatcatt
     2221 ggaaaaaaga taaaaaatat tttgagtttc aatatcagaa taatcaagta tatctataaa
     2281 ctccttaatt tcagatatgg taattttcag gccaattgat tcggttccat acttaagggt
     2341 tttatgatca taaacaatat ttatttttga aaaaattaca actgccagca tatccaaaag
     2401 acagtctatt acttcataat catctgtttc ttcaagaaaa gatattattt gaagaaaatc
     2461 tgttttggtc tggagctttg aaaaaatttc ttctttaatc ttccggatat tttcattttt
     2521 ggtcagatcc tttcctagaa aactgtaaag ggtcttcaga gtatggtagt acattttatc
     2581 cccctgtctg caaaaataac tgccatttat tgtataatct attataacac atatataaca
     2641 tataaaattc ggaggaattt ccatgattaa acaagttacg gtagatgaaa aaaactatta
     2701 caagcgactg gataaatttt tacgaaataa tttttccgaa cttaaactgg gaactatatt
     2761 tatgcttata agaaaaggaa atgtaaaaat aaacggtaag aaagcaaagc agaatgaaac
     2821 cgaacttttt ataggcgatg atgtccaaat atttattaat gatcagcttg aagatatttc
     2881 tagaccggaa gttccgcagt ttaagccaag atatatggat cttaaagttc tttatgaaga
     2941 tgagtatgta attgctgtgg ataagcctcc gaaagtttct atgcatcctg gtagatcaga
     3001 agagatggta acccttatag aagctcttat attcagagca tctcaagagt atgagcctca
     3061 tctagtacac cgtttggaca agcatacttc tggagtggtt gttgtagcaa agacaaagca
     3121 gattgcgcga gagcttactg aaataataat gaatagagaa acagacaaat attatatagc
     3181 tttattgagc aaaaatctta aaaacgctaa ggggactctt aactccattg ttgatgaaaa
     3241 ggatgctgct cttgagtata ctgttgaaaa tgtctataaa aatcttatgg gaagttttcc
     3301 tctggtcaat gtacatcttc ttactggaag aaaacatcag ataagagttc agttttctca
     3361 aatatcgtct cctgttcttg gagatgatat ttatggagat aaagattata acagacaggc
     3421 aaaaaatctt tttggtttga aaaggta
//
LOCUS       DAOSHR010000209         3396 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_26016_length_3396_cov_5.696199, whole genome shotgun sequence.
ACCESSION   DAOSHR010000209 DAOSHR010000000
VERSION     DAOSHR010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 3396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3396
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26016_length_3396_cov_5.696199"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2984
                     /locus_tag="PLU28_09565"
     CDS             <1..2984
                     /locus_tag="PLU28_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35344.1"
                     /translation="DVQGVSLLIRNPTEAKVIDLGRNDLEYLFHTTKGATATVMLIYD
                     KNGDNTYDEPPTEIIKLDTSKAKETQIPEVASTVYEYSGSMSTADLKRFPNTGKYRVK
                     VISKIGKFEVERYSNINVRDTYAPVLKKNDGLLLVSKNIRSVYPKLSEGMENGTLFEK
                     TESTGLKRYYYIPIDFKNGKNNTDVDITIKALDYGNITKAEYYIDSVLQPLPELTSVE
                     LVDSQADVGAKQRMFTYKATIPKDKLDYQVKELKIKLYDVEHPDGIEEIFYVKGVEIE
                     KPVLNEFKVNFSEGPSDLKELTINKSDTGTFAISAGGEYKVSITNIDFSDNLYIGAVV
                     LKIFDNDRNTERTLKTWTASIDSPQIKTPISFEPDGLSSGALKIKMPDLIGNYTLKVE
                     LLDKTYLQVKGTKFEKDSQNLNRDIAEFKVNVIDTRPLAVSLKLENKTYKNDMRIISS
                     PFNLYINAQGRRDMIETSSIKVNMVMPDGTTKILDKTELTPIGEELLYSVGASTLRPG
                     VDKDGICTLYINYATKNRPSEIKKSPEYKVVLDLNQNPYIGDIVMENTSGRLVLKSTI
                     ANTLSYDIDDVMLFYKPIGSAYYKSIPGSISANEVFFEIGVLSIGRYDYYVRVTDKSG
                     RTRFSPDKQFSVDSKTPTIDNFQAFGNNTGKYSFRSTDTEKIITARDSYGISKVLVEA
                     YYKDDSIPKEYSYIKVFTASIPSEAVFDKDGKSVKILTKAFQDNLKLQSGKPVEAYNM
                     TVTVFNTQSLSKSFSLVINDDDLKPSAGVLSLPSAGTIISESNYGQNEIRALITEDTG
                     ISYVTLEISHPNFLNVDNSFKTQYMQAQESNSANQYPYSYKITNLHKTDLYEPVTEGE
                     FDVTIHAWDLAGNTESFNLGKIVVDLKPPVIQSFTLNNPQITFAQYNSSDEILTAELR
                     DFTISNSQAYIIIDTVQYDISSLMHCVGDTAGISFTAVNLKNILGNISSRRVVTFKIK
                     VIDGAGHETVYRDNLTLVIAP"
     gene            2999..>3396
                     /locus_tag="PLU28_09570"
     CDS             2999..>3396
                     /locus_tag="PLU28_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35345.1"
                     /translation="MNKKNILIIIICSVFIFLFYGCMLFNDVQSPVFLSDPVLRVIPD
                     RAGAFMEFDLKVQDNVAISKIELFADNSSVPFTVPLGADKPYGQKEYRGSIKVPAPVS
                     DAGRFYEVTVKVYDTAGNPPVSRKLDKRLMT"
BASE COUNT         1118 a          608 c          716 g          954 t
ORIGIN      
        1 tcgatgttca gggtgtttcc cttctcataa gaaatccaac agaagcgaag gtaatagatc
       61 ttggcaggaa tgatcttgag taccttttcc atacaaccaa aggagctacc gctacagtta
      121 tgctcatata cgataaaaac ggtgacaata cctatgatga gcctcctacg gaaattataa
      181 agcttgatac atctaaagct aaggaaacac agattccgga agttgcgtca actgtatatg
      241 agtattccgg gtctatgtct acagccgatc tgaaaagatt ccccaataca gggaagtata
      301 gggtgaaggt tatttcaaaa ataggaaagt ttgaggttga gagatactca aacataaatg
      361 tcagagatac ctatgctcct gtgttaaaga aaaatgacgg cttgcttctt gtatccaaaa
      421 atatccgttc agtctatccg aagctttcgg agggaatgga aaacgggaca ctttttgaaa
      481 agactgaaag tacaggtctg aagaggtact actacatacc tatagacttt aaaaacggaa
      541 aaaataatac cgatgtcgat ataaccataa aggctcttga ttatggaaac ataaccaaag
      601 cagagtacta tattgacagt gttttacagc ctcttcctga gcttacatct gtagaactgg
      661 ttgattctca ggctgatgtg ggggcaaagc aaaggatgtt tacatataaa gctactatac
      721 ctaaagataa gttagattat caggtaaaag agcttaaaat aaagctttat gatgttgagc
      781 atcctgatgg aatagaggaa atattttatg tcaagggtgt agagatagaa aaaccggttc
      841 ttaacgagtt taaggttaat ttctcagaag gcccttcaga tcttaaggaa ctgaccataa
      901 acaaatccga taccggaacg tttgccattt cagctggcgg cgagtacaag gtaagtatta
      961 ccaatataga tttttctgat aatctgtata taggagccgt agtactcaag atttttgata
     1021 atgacagaaa cacagaaaga actcttaaga catggacagc gtcgatagac agtcctcaga
     1081 taaaaacgcc tatctctttt gagcctgacg ggcttagttc gggggcgctg aagattaaga
     1141 tgcctgatct tataggtaat tacaccctga aggttgagct tttggataaa acgtatcttc
     1201 aggtaaaagg aaccaagttt gaaaaagatt ctcagaatct gaacagagat attgccgaat
     1261 ttaaggttaa tgtgattgat acaagacctt tggcggtaag tctgaagctt gagaataaaa
     1321 catataaaaa cgatatgaga ataatatctt ctcccttcaa tctttatata aacgcacagg
     1381 gcaggaggga tatgatagaa acttcaagta taaaagtaaa tatggtgatg cctgacggaa
     1441 caaccaaaat actggataag acagaactga cccccatagg cgaagagttg ctctactctg
     1501 taggtgcaag cactttgcgt ccgggcgttg ataaggacgg aatatgtact ctttatatta
     1561 actatgcgac aaaaaacaga ccgtctgaga taaaaaaatc accggagtac aaggtagttc
     1621 ttgatctcaa tcaaaatccg tacattggag atattgtcat ggagaatact tcaggcagac
     1681 tggttctaaa atcaaccatt gccaatacac tttcatacga tatagatgac gtgatgcttt
     1741 tctataagcc tataggatca gcatactata aaagcatacc cggaagcata agtgcaaatg
     1801 aagtgttttt tgagatagga gtactgtcaa tcggaagata tgactactat gtaagagtta
     1861 ccgataaatc cggaagaaca aggttttctc cagacaaaca gttttcggtt gactccaaaa
     1921 ctcctacgat agataatttt caggcatttg gaaataatac agggaagtat tcattcagat
     1981 caaccgatac tgaaaaaatc attacggcca gagacagcta tggaatatca aaagttctgg
     2041 tggaggctta ttacaaagat gactccattc ctaaagagta cagttatata aaggtattta
     2101 ccgccagcat tccttcagaa gcagtctttg acaaagacgg taaatctgta aaaattttaa
     2161 ccaaagcatt tcaggataat cttaagctgc agtcaggcaa gccggtggaa gcttataata
     2221 tgacggtaac ggtttttaat actcaaagtc tgtcaaaatc gttcagtctt gtaataaacg
     2281 atgatgactt aaaaccatct gccggtgtcc tctcgttgcc ttcggcaggt actataataa
     2341 gcgaaagcaa ttacggccag aatgagattc gggctcttat aaccgaagat accggtattt
     2401 catacgttac tctggagata tcacatccga acttcttgaa cgttgataat tcttttaaaa
     2461 ctcagtacat gcaggcacag gagagtaatt ccgctaatca gtatccttat tcatacaaaa
     2521 taaccaatct gcataagaca gatctttatg aaccagttac agaaggcgag tttgatgtta
     2581 ctatacatgc ttgggatctt gctggcaata ccgaaagttt caatctgggt aagatcgttg
     2641 tagaccttaa gcctcctgtt atacagagtt tcactcttaa taacccgcag atcacttttg
     2701 cacagtacaa ctcctccgat gagattctta cggcggaact gagagatttt acaatatcaa
     2761 attcacaggc atatataata attgatacgg tgcagtacga tatatcttca ctgatgcatt
     2821 gtgtaggaga tactgcaggt atatccttta ccgctgtgaa tctgaaaaat attctgggaa
     2881 atatctcctc cagacgtgtg gttactttta aaatcaaagt aatagacggt gcggggcacg
     2941 aaacagttta ccgtgataac cttactttgg ttattgcgcc ttaggcgaga ggtgaggtat
     3001 gaataaaaaa aatatactga ttattattat ctgttcggtt tttatatttt tattttacgg
     3061 ctgtatgctc tttaatgatg ttcagagtcc tgtcttttta agcgatcctg ttttgagggt
     3121 tattcctgat agggctggag cttttatgga gtttgattta aaagtccagg acaatgtggc
     3181 tataagcaag atagagcttt ttgccgataa ctcttcggtt cctttcacgg tgcccttggg
     3241 agcagataag ccttatgggc agaaggagta caggggctca ataaaagttc ctgcgcctgt
     3301 atcggacgcc ggaagatttt atgaggttac agtaaaagtt tacgatactg ccgggaatcc
     3361 tccagtaagc agaaagcttg ataaaaggct tatgac
//
LOCUS       DAOSHR010000210         3388 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_26094_length_3388_cov_4.423942, whole genome shotgun sequence.
ACCESSION   DAOSHR010000210 DAOSHR010000000
VERSION     DAOSHR010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26094_length_3388_cov_4.423942"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="PLU28_09575"
     CDS             <1..594
                     /locus_tag="PLU28_09575"
                     /inference="COORDINATES: protein motif:HMM:NF019869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOG35346.1"
                     /translation="KLMHPFMPFLTEELWHALPGIGEEQMIISALWPQYDEKFSFEDE
                     TSVFSKAMEVVRGIRNVKAEMNILQTKSVDITYVSDHKETVIDQMKEQIMRLGFVSNI
                     ERSQAKPSRAATAYVGEGMEVYIPLGELIDFDAEKERLEKAKEKALKDVEKYQKKLQN
                     KDFLERAEEEVIEETRTKLEDSQSQYDKVLKILQELT"
     gene            597..1910
                     /locus_tag="PLU28_09580"
     CDS             597..1910
                     /locus_tag="PLU28_09580"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HOG35347.1"
                     /translation="MTFKEAVDFLYVKRGETKIKLGLERITQLCELIGNPQNTFKSVH
                     ITGTNGKGSVTKALTDIFIGQGYTVGIYTSPHLMSVRERIRVNNRNISENDFVRILED
                     MLPSIEEMDAMPGNMSPSFFEITTAMAFKYFAEKKVRFGMIEVGMGGRFDATNVLNSD
                     VSVITTIQRDHMKILGETPEEIAFEKAGIIKNNNYVVLGDISESSKGVILRKAASVQS
                     AKVFQFNKDYRFENPRYTLNWNTMDYYGLGFNLKDLSYKANGTYQPHNITVALAAAEC
                     LAQKNGVSLNEEKLRASMKQFYWEGRFEILEYKGKKIVLEGAHNEDGAKVFAKTVGIY
                     MPLNRKVAMIGILNDKDYLHMADYLSKLFEKTIITRVPSERSNDPKDVYDTFKNLGTK
                     NISFENDFEIAFEKLLSESADYYFISGSLYLVGAIRALIMGVKGP"
     gene            1912..2475
                     /locus_tag="PLU28_09585"
     CDS             1912..2475
                     /locus_tag="PLU28_09585"
                     /inference="COORDINATES: protein motif:HMM:NF025873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOG35348.1"
                     /translation="MLRRLNGIIVEVLEDSVLIQTGDFTFQAVPSFRVLKEFSPDERF
                     NYEAILEINEYGTALFLFRDTLERETFDTLRKVSKLGPKTCGKIIKSVDSEVLRNIIA
                     GGDIDKLSKVPGVGKKTAERIVTELKGEFENLDIHQREDFSADAVEAMLALGFDKYSV
                     LKALKGIKTENLPTEEIIKLLLTKVKR"
     gene            complement(2541..2996)
                     /locus_tag="PLU28_09590"
     CDS             complement(2541..2996)
                     /locus_tag="PLU28_09590"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOG35349.1"
                     /translation="MKALRKYGNYNEEDYLTPFDLLRKEVDSLFGSFPFESAYQSDFM
                     VPKMDIYENENAVVVETEMPGVDKKDIKIKLEGDILSVSAEKKSDDEKKNRNFFRKER
                     YYGKFERTLRLPEYIDPQKISAKYEGGVLEIQIEKKPEAQKVSKEITIQ"
BASE COUNT         1098 a          616 c          654 g         1020 t
ORIGIN      
        1 aagcttatgc atccgtttat gccgtttctg acagaagagc tctggcatgc acttcccggg
       61 ataggcgaag aacagatgat aatttctgcc ttatggccgc agtacgatga aaaattcagc
      121 tttgaggatg aaacatcagt attttctaag gctatggaag ttgtacgcgg aataaggaac
      181 gtcaaagccg agatgaacat acttcagact aagagtgtag atataacgta tgtctcagat
      241 cataaagaaa cagttataga ccagatgaag gaacagataa tgagactggg ttttgtatca
      301 aatatagaaa gatcccaggc aaagccttca agagctgcta ccgcttatgt cggagaagga
      361 atggaagttt atatacccct tggcgagctt atagacttcg atgcagaaaa ggaaagactt
      421 gagaaagcta aggagaaagc tctcaaggat gttgaaaaat atcagaaaaa acttcagaat
      481 aaagatttcc ttgaaagagc cgaggaagag gtaatagaag aaaccagaac caaactggaa
      541 gactctcaga gccaatacga taaagttcta aaaattttac aggagttgac ctaaatatga
      601 catttaaaga agctgttgac tttctatatg taaaaagggg agaaaccaaa ataaagctcg
      661 gtttagaacg gataacacag ttatgtgagc ttataggcaa ccctcagaac actttcaaat
      721 ctgtccatat aaccggcact aatggaaaag gcagtgtaac caaagctttg accgatatat
      781 ttatcggaca gggctatacg gttgggatat atacttctcc tcatcttatg agtgtacgcg
      841 aacgtataag agtaaataac agaaatattt ccgaaaatga ctttgtaaga atactcgagg
      901 atatgcttcc ttctatagaa gagatggatg ccatgcccgg aaatatgtct ccttcatttt
      961 ttgaaataac gacagccatg gccttcaaat attttgctga aaaaaaagta aggttcggca
     1021 tgattgaagt aggaatgggt ggacggtttg atgccacaaa cgtacttaat tcagatgttt
     1081 ccgtaataac cacgattcag cgtgaccata tgaaaatact cggcgaaacc cctgaagaaa
     1141 tcgcatttga aaaagctgga ataataaaaa ataataacta tgtcgtcttg ggagatatct
     1201 cggaatcctc caaaggagtg atacttagaa aagctgcctc agtacagtct gccaaagtat
     1261 ttcagttcaa caaagattat aggtttgaaa atcccagata taccctgaac tggaacacca
     1321 tggactacta cggattgggt ttcaatctta aagacttgtc ttataaagcc aacggtacat
     1381 atcagccgca taatataaca gtcgctttag ccgccgcaga atgtcttgca cagaaaaatg
     1441 gcgtttcttt gaacgaggaa aagttacgag ccagcatgaa acagttctat tgggaaggac
     1501 gatttgaaat acttgaatat aaaggaaaaa agatcgttct tgaaggggct cacaacgaag
     1561 acggtgcaaa ggtttttgcc aagacggtcg ggatatatat gcctttgaac aggaaagttg
     1621 ccatgatagg tattctcaac gataaggact atctgcatat ggctgactat ttatccaaac
     1681 tttttgagaa aaccataata acccgtgttc ccagtgaacg ttcaaatgat ccgaaagatg
     1741 tgtacgatac ttttaaaaat cttggaacaa aaaatatatc ttttgaaaac gattttgaaa
     1801 tagcttttga aaaactgctt tcagaaagtg cagattatta ttttataagc ggatcacttt
     1861 accttgtcgg agctatacgg gcgctcatca tgggcgtaaa gggaccttga catgctccgc
     1921 aggctcaacg gtataattgt tgaggttctt gaggattctg ttctgataca gacaggtgac
     1981 tttacctttc aggccgtacc ttcattcagg gttttgaaag aattttctcc tgatgaaagg
     2041 ttcaattatg aagccatact tgaaataaat gagtacggga cagctctttt tttattcaga
     2101 gataccttag aaagagagac tttcgatact ttaagaaagg tttccaagct tggtccgaag
     2161 acctgcggca agataataaa atcagtggac tcggaagttc tgcgcaatat catagctggg
     2221 ggagatatag ataaactgtc aaaagtaccg ggagtaggga aaaaaactgc tgagagaatc
     2281 gtaacagaac ttaaaggtga atttgaaaat cttgatatcc accaaaggga agatttttcg
     2341 gctgatgctg tagaggctat gttggctctg ggctttgata aatattccgt tcttaaggca
     2401 ctcaaaggaa taaaaaccga aaacctgccc accgaagaaa taataaagct gcttttgacc
     2461 aaagtaaaga gataatccgg caattattaa taaaaagaac ggcatacgat gccgttcttt
     2521 ttgttgcttt aagcttatcg ttactgtatg gttatttctt ttgaaacctt ctgtgcttct
     2581 ggcttttttt ctatctgtat ttcaagcaca ccaccttcat actttgcact tatcttctga
     2641 ggatcaatat actcaggcag tctgagagtt ctttcaaact ttccgtaata tctttctttt
     2701 ctgaagaaat tcctgttttt cttttcatcg tcacttttct tttcggctga tacactcagt
     2761 atatctcctt caagctttat ctttatatcc tttttatcga ctcctggcat ctctgtttca
     2821 accacaactg cattctcatt ttcatatata tccatcttcg gaaccatgaa atctgactga
     2881 taagccgatt caaaaggaaa agagccgaaa agactgtcaa cttcttttct gagcagatca
     2941 aacggtgtca aataatcctc ttcgttatag tttccatact ttcttaatgc tttcatactc
     3001 aatccctcct tatattttta ggttgcttct ttatattgtt atcttttcac tttcaatata
     3061 atattaccat tatcactcta tattgtcaag tgctaattgt gatttttgag aattgttata
     3121 cgtgcctttt tattagcgat ataattcaat aaatattttt gtatgaatat atttaagcat
     3181 acgttactta taattgccaa aaaacattaa aattataccc gccgtttgaa ataaaaacga
     3241 cgggtattga ttattcttca ataatttctt ctgtatattc ctttaattcc atctcttgaa
     3301 gtatctcgga tatttttctg ccatcaactg attttatgta ctctgaggca taatacacct
     3361 tgtatccgaa ctttatggca tcctttat
//
LOCUS       DAOSHR010000211         3385 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_26130_length_3385_cov_5.060661, whole genome shotgun sequence.
ACCESSION   DAOSHR010000211 DAOSHR010000000
VERSION     DAOSHR010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 3385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3385
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26130_length_3385_cov_5.060661"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="PLU28_09595"
     CDS             <1..408
                     /locus_tag="PLU28_09595"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG35350.1"
                     /translation="DIYEIDRVEYKYRLKRIIADFQLEELMNIPVRKLSLGQRIRCEI
                     AVSILHEPKILFLDEPTIGLDVVVKNQIRELIHDMNLKTGMTVFLTSHDVGDIEKLCK
                     RILIIDKGKVILESTTYRLKHEFLKKKDSFAQA"
     gene            612..1400
                     /locus_tag="PLU28_09600"
     CDS             612..1400
                     /locus_tag="PLU28_09600"
                     /inference="COORDINATES: protein motif:HMM:NF017946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HOG35351.1"
                     /translation="MAKLKKYFKIFSITFLNDIKYSKNFISQFLFFGLFIYVFSQLWS
                     AVYSERASIEGFDFYKMLWYFMFTESIMLSRPALHREMSDAIKSGDIVYTLNKPYSYI
                     LFCYSKFMSSALVRMFINMVVGSLLLFMILRTCPLTLLGTIRGVVSAFLGLSLNFLFF
                     FLIGLSSFWFEDNSAFVFIYSKFLFTVGGLFVPLEFFPSWIKGFAQILPFQYILYAPS
                     KLFMSGGTDFFGIIGLQIINIIIYSAIVTGIFRYALKRLNVNGG"
     gene            1403..2239
                     /locus_tag="PLU28_09605"
     CDS             1403..2239
                     /locus_tag="PLU28_09605"
                     /inference="COORDINATES: protein motif:HMM:NF017946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HOG35352.1"
                     /translation="MKAFKLMQSIKTFRLVQSIKTFRLIIRYLKFNLSSQMEYRGNFI
                     LQTIGMVINDVFLLFFWWILFQKVGTIKDYGMNEMLLVYSITTFSFGISMTLWGNQSL
                     ISRMIMNGEIDSFLILPRPVLSHILVSRLSLSALGDLVFGFVLFFFSGYLSLLNFLLF
                     ILLGVMGAVLLSAYNVLFHSFTFWYGDFTAVAEVIPDSIITFSLNPEGIYTSVSKLII
                     QTVIPAMYFSFLPVRLIKEFSMVSFLFLLIADMSFVLAAYAVFYKGLKRYESGNLISK
                     KI"
     gene            2296..2946
                     /locus_tag="PLU28_09610"
     CDS             2296..2946
                     /locus_tag="PLU28_09610"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HOG35353.1"
                     /translation="MFCPNCGAQVENGDRFCKNCGKDLSTSVFKGDVQNTSVNENHYN
                     PVRAKSYGGFWKRLVACIIDGLIIGVINWFVSAFMGLGIGFRFLSDIFNEMTYYNDYN
                     WGYNYGNVPNFFEAALRIVVGSVTISLVTTVVSWVYYAGFESSSFKATPGKLVLGMEV
                     TDLDGQRISFLRATGRYFAKIISGLILFIGFIMAAFTEKKQALHDIIAGCFVVNKN"
     gene            3036..>3385
                     /locus_tag="PLU28_09615"
     CDS             3036..>3385
                     /locus_tag="PLU28_09615"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HOG35354.1"
                     /translation="MANYYEILGIPKDAKYNEIENAYSEKIKEYLHKLNDHSYAKSYK
                     KLEEELTAVNKAYKTISDPKLKTAYDKKIPNVVIINTRKPKGKIPSALVMSTFLITFF
                     LGVIGHGANPVYYI"
BASE COUNT         1035 a          536 c          693 g         1121 t
ORIGIN      
        1 gatatatacg agatagacag agttgagtat aagtaccgtc taaagagaat aattgcagat
       61 tttcaacttg aagaattaat gaatattcct gtgaggaagc tgtctctggg tcagcggata
      121 agatgtgaaa tagcggtcag cattcttcat gaaccaaaga ttcttttcct tgacgaacct
      181 accataggac tggatgtagt tgtaaaaaat cagatacgcg agcttataca tgatatgaat
      241 cttaaaaccg gtatgactgt ttttcttaca agccatgatg tgggggacat tgaaaagtta
      301 tgtaagagga tacttataat agacaaaggg aaagtgattc ttgaaagcac aacttacaga
      361 cttaagcatg aatttttgaa aaaaaaagat tctttcgctc aagcttgaca gcgagtatat
      421 accccagcac gacggaataa gcgttctaaa atcaaaaggt acagggttaa agctgtcggt
      481 tgacacatca aaagttcaga tacagtatgt tatacaaaat attctgtctc gaactggcat
      541 tcaggatatg aatatttcgg atacgcccat ggaagatgta ataagaaaga tctataccgg
      601 agatgattca gatggccaaa ctcaagaagt actttaaaat tttcagtatc acatttttaa
      661 acgatataaa gtactccaaa aattttataa gccagtttct tttcttcgga cttttcatct
      721 atgttttttc acagctatgg agtgcggtct attcagaaag ggcaagcatc gaaggatttg
      781 acttttacaa aatgctttgg tatttcatgt ttacagaatc tataatgctt tcaaggcctg
      841 ctctgcacag agagatgtct gatgccataa aatcgggcga tatcgtttat actttaaaca
      901 agccgtactc atatattttg ttttgctaca gcaagtttat gtcatcagcg cttgtaagaa
      961 tgtttataaa tatggttgtc ggatctttgc ttctttttat gatactcagg acatgtcctc
     1021 tcactttgct tggaacaata aggggagttg tgagcgcctt tttggggctg agtctgaact
     1081 ttcttttttt ctttctgata gggctttcct cattttggtt tgaagacaac agtgcctttg
     1141 tattcatcta ctcaaagttt ttgtttacag taggagggct ttttgtgccg ctggaatttt
     1201 ttccgtcatg gataaaaggt tttgctcaaa ttctgccctt tcagtatatt ctatatgctc
     1261 cgtcaaaatt atttatgtcg ggtggaacag atttttttgg aataatagga ttgcagatta
     1321 taaatattat aatatactca gccatagtga cgggtatttt cagatatgcc ctgaaaaggc
     1381 ttaatgtaaa cggagggtaa ggatgaaagc atttaaattg atgcaaagca taaagacatt
     1441 tcgactggtg caaagcataa aaacatttcg actaattatc aggtatctta agttcaacct
     1501 ttcttctcag atggagtaca gaggtaactt tattctccag acaatcggga tggtaataaa
     1561 tgatgtgttt cttctttttt tctggtggat tctttttcag aaagtaggaa caataaagga
     1621 ttacggtatg aatgagatgc ttttggtata tagcataacc actttttcgt tcgggataag
     1681 catgacgcta tggggtaatc agtctctgat aagccgtatg ataatgaacg gcgagatcga
     1741 ttcatttttg attcttccaa gaccggttct ttcccatatt cttgtgagca gactttcact
     1801 gagtgcttta ggtgatttgg tattcggatt tgttcttttc tttttttctg gatatctgtc
     1861 gttgttaaac tttctgcttt ttatactact tggagtaatg ggggcggttc ttctttccgc
     1921 ctataatgtg ctttttcatt cttttacctt ctggtatgga gattttacgg ctgttgcgga
     1981 agtgataccc gattccataa ttactttttc ccttaatccg gagggaattt atacatctgt
     2041 ttcaaagctg ataattcaga cggttattcc ggccatgtac ttttcctttc ttccggttcg
     2101 acttataaaa gagttcagca tggtttcctt tctgtttctt ctgatagccg acatgagctt
     2161 tgtacttgct gcatatgcag ttttttataa agggctgaaa agatatgaat ccggtaatct
     2221 tatatctaaa aaaatataaa caaccaataa atatgataca atttagtata taaaacttta
     2281 agagggaggt aaaaaatgtt ttgtcctaac tgcggtgcac aagtagagaa tggagacaga
     2341 ttctgcaaaa actgtggaaa agacttatca acatctgttt ttaaaggaga tgtccagaat
     2401 acttctgtca atgaaaatca ttataatcca gttcgtgcga aaagctacgg aggtttctgg
     2461 aaaagacttg tagcttgtat aattgatggt ctgataatag gagtgataaa ctggtttgtc
     2521 tcggctttta tgggtttagg aatagggttc aggtttctta gcgatatttt caatgagatg
     2581 acatactata atgattataa ctggggatac aattacggga atgttcccaa tttttttgaa
     2641 gcagctctga ggattgttgt gggatctgtt actataagtc ttgttacaac ggttgtttcg
     2701 tgggtatact atgcaggatt tgaaagcagc agttttaaag cgactcccgg taagcttgtg
     2761 cttggaatgg aagtaacgga tcttgacggg cagcggatat catttttaag agctacagga
     2821 aggtatttcg caaaaatcat atccggatta attcttttta taggttttat aatggctgct
     2881 tttacggaga aaaaacaggc tctccacgat ataattgccg gatgttttgt cgttaataaa
     2941 aattagaggc caagcctctg atttttattt ttaggcaaag atttataatt taatagtagt
     3001 atgctataat agagcagaaa aggggggaag aatttatggc aaattactat gaaatacttg
     3061 gaattcctaa ggatgctaag tataacgaaa tagaaaatgc ttacagcgaa aagataaagg
     3121 agtatttgca caagctgaat gaccacagtt acgccaaatc gtataaaaaa cttgaagaag
     3181 aacttacggc cgttaacaaa gcatacaaaa cgatttcaga tcccaaactg aaaacggcat
     3241 acgataaaaa aattccaaat gttgtcatca taaacacccg taagcctaaa ggaaaaatcc
     3301 cttcagcact tgtgatgtca acatttttga taactttttt tcttggagtc ataggtcatg
     3361 gtgcaaatcc tgtttattat ataag
//
LOCUS       DAOSHR010000212         3362 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_26385_length_3362_cov_4.998186, whole genome shotgun sequence.
ACCESSION   DAOSHR010000212 DAOSHR010000000
VERSION     DAOSHR010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3362
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26385_length_3362_cov_4.998186"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1081)
                     /locus_tag="PLU28_09620"
     CDS             complement(<1..1081)
                     /locus_tag="PLU28_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG35355.1"
                     /translation="MTIGQFLFGTGVLHSVEKPSRYIGGELNQVKKENPQVRIALCFP
                     DLYDVGMSHTGFHILYSLFNQNEAVACERVFLPWMDMIEKLEKSDIPLYTLESLTPVK
                     DVDMLGITLQYELVYTNVIKVLQLSKIPVLSSDRTQNDPIVIGGGPCVSNPLPVKDFF
                     DAFFIGDGETVYEKIAEIFRSTSCREERLQLLSKTPGIYVPKYYSTIKVTAQKVTDLD
                     RYSDLTRQVIPHFEVVHDRAIVEVMRGCNRGCRFCHAGMFYRPVRERDPKNIIDNAMK
                     SLKNTGYEEISFLSLSTLDYSELSVVLDTLMPYFRENRISVSLPSSRVDKFGIEITDK
                     LGGGRRAGLTFAPEAGSQKMRDVINK"
     gene            complement(1127..2065)
                     /gene="fba"
                     /locus_tag="PLU28_09625"
     CDS             complement(1127..2065)
                     /gene="fba"
                     /locus_tag="PLU28_09625"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HOG35356.1"
                     /translation="MSYVNTKVILDRANKEYYGVGAFNINNLEFAQSILEACDEKKSP
                     CILQTSEGAVKYAGNGDALRGARFFVGMVELYAQTLDIPVALHLDHGTDLKYIMACIK
                     AGYSSVMIDASHFKFQENLEKTKEIVKIAHAAGVSVEAELGQLAGIEDNIQAAESVLV
                     NPNEAKVFVEETEVDFLAPAIGTSHGAFKFKGDAKLDYERLAKVKEITGMPLVLHGAS
                     SVVQEMVAIAQKYGADFGGSKGVPSDVLKETVKFGINKVNTDTDLRMSFIAGLREFLA
                     NNPKEFDPRKYQKTAKEYVKKVVMDRIDVLGSFGKA"
     gene            2241..>3362
                     /locus_tag="PLU28_09630"
     CDS             2241..>3362
                     /locus_tag="PLU28_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35357.1"
                     /translation="MPKIKREKAVEVTQEKEDVVINEELLMDFVKTFLTYDLLMCKVK
                     EEVIEEALKRINFSEVKNNKDKFLSDIQDKSDSLVVDYNRYEYMNKYYNSDGFFLPDD
                     DDNEGWDEIYQEEMSKIDEQMHNRDDQENDYEDEAFEETIEIYDKNKEAKYYDEFYKN
                     IEELFYYYYVYFELIYIYSKRTKLCYGNQSTEVWFDKELIKLDDVYNGLTVDDYLNES
                     NPKPKRSFVANAFSDKIDNLEELAINTAKKLATNHLLALGIDKDSIEKRLSKYTKTPT
                     PEELEEEICSFRHLSDHFADEYKNSVDSNFIENYNDSFWSKHNRVVKTKKSHLKKPLL
                     KPLIYDDDIISSDIPELDLFLPADENITETTKTEQKNKDR"
BASE COUNT         1122 a          642 c          567 g         1031 t
ORIGIN      
        1 ccttatttat aacgtctctc atcttttgag aacctgcttc aggtgcaaaa gtaaggcctg
       61 cacgccttcc accaccgagt ttatcagtta tttctattcc gaacttatct actctgcttg
      121 aaggaaggga aacgcttatc ctgttttctc tgaagtatgg catcaatgtg tccagaacca
      181 cagacagttc ggaataatca agtgtgctta acgaaagaaa ggaaatctct tcataccctg
      241 tattttttaa tgatttcatg gcattgtcta taatattttt cgggtctctt tccctgacag
      301 gtctgtagaa catgccggca tggcagaacc tgcagccgcg gttacagcct ctcattactt
      361 caacaattgc tctgtcatga accacttcaa aatgaggtat tacctgtctg gtaagatcag
      421 aatacctatc caaatcagta accttctgcg cagttacctt aattgtcgaa taatattttg
      481 ggacatatat tcctggagtt ttagacagaa gctgaagcct ttcctccctg caggaagtac
      541 ttctgaaaat ttcagcaatc ttttcataca cagtctcccc gtcacctata aaaaaggcgt
      601 caaaaaaatc ttttaccggc aaaggattag aaacacaagg tcccccacct attacaatag
      661 gatcgttttg cgttctgtcc gaactaagaa ctggtatttt tgaaagctga agaaccttta
      721 taacgtttgt atacaccaac tcatactgaa gagttatacc aagcatgtcg acatctttta
      781 ccggggtgag actttcgagt gtataaaggg gtatatcgct cttttcgagt ttttcaatca
      841 tatccatcca cggaagaaat accctttcac aagccacagc ttcattctgg ttgaaaagac
      901 tgtaaagaat atgaaagcct gtatgagaca tgcctacatc ataaagatcc ggaaaacaca
      961 aagctatcct gacctgcgga ttctcttttt ttacctgatt taactctccg ccgatatatc
     1021 ttgacggttt ttccactgag tgaagaacac ccgttccaaa aagaaactgt ccgattgtca
     1081 tactctctcc attctacaaa aatccccctc aacaaggggg atgattttag gcttttccaa
     1141 aagaaccaag tacatctatt ctgtccatga ccactttttt tacatactct ttggctgttt
     1201 tctgatactt tctgggatcg aactccttcg gattgttagc aaggaattct cttaatccgg
     1261 ctataaaaga cattctaaga tctgtatctg tattcacttt attgattccg aacttaaccg
     1321 tttctttcaa aacatctgaa ggaacacctt tggatccgcc aaagtctgcc ccgtacttct
     1381 gcgctattgc aaccatttcc tgaacaacac tcgaagcgcc atgaagaacc aaaggcatac
     1441 ctgttatctc ttttactttt gccaatcttt cataatcaag tttagcatca cctttaaatt
     1501 taaaagctcc gtgtgatgta cctatagcag gagccagaaa gtcaacttcg gtttcttcaa
     1561 caaaaacctt agcctcgtta ggatttacaa gaaccgattc ggcagcctgt atgttgtctt
     1621 ctattcccgc cagctgaccg agctctgcct caacagatac tcctgctgca tgggctattt
     1681 ttacaatttc ctttgttttt tcaagattct cttgaaattt aaaatgcgag gcatcaatca
     1741 tgaccgaaga gtatccggct ttaatacatg ccattatata ctttagatct gttccatggt
     1801 caagatggag ggcgaccgga atgtccagtg tctgcgcata aagttccacc attcctacaa
     1861 aaaatcttgc tcctctaagt gcatcaccgt ttcctgcata ttttactgca ccttcgctgg
     1921 tttgaagaat acaaggagat tttttttcgt cacaggcttc gagtatcgac tgggcaaact
     1981 caagattgtt tatattgaat gctcctactc cataatactc tttgttagct ctgtccaaga
     2041 ttaccttcgt attgacgtaa gacattatat tacctcctat actatttttt acaatggaat
     2101 tatagcatac tatttttaac gttgtatatc gacggaaaag attaaaaatg gagctcgcaa
     2161 aaaaataaat aatacgctat tggtttttag gcaattgtct actaaactgc aacagtaaag
     2221 ccttatcatg aggaggaact atgccaaaaa taaaacgcga gaaagctgtt gaagttactc
     2281 aagaaaaaga agatgttgta atcaacgaag agttacttat ggatttcgta aaaacttttt
     2341 taacatatga tttgcttatg tgcaaagtaa aagaagaagt tatagaagaa gctttaaaaa
     2401 gaataaattt tagtgaggtc aaaaacaata aagataaatt cctttcggat atacaagata
     2461 aatcagattc tcttgttgtt gattataacc gatacgaata tatgaataaa tattataatt
     2521 ctgatggttt ctttcttcct gatgatgatg acaatgaagg ctgggatgaa atatatcaag
     2581 aagaaatgag caaaatagat gaacaaatgc ataatagaga tgaccaagaa aatgattatg
     2641 aggatgaagc atttgaagaa acgatagaaa tttatgataa aaataaagaa gctaaatatt
     2701 atgatgagtt ctataaaaac atagaagagt tattttatta ctactatgta tactttgaac
     2761 ttatctatat atattcaaaa agaacaaaac tttgttatgg gaatcaatct actgaagtat
     2821 ggtttgataa agaactgatt aaattagatg atgtttataa tggtttaaca gttgatgatt
     2881 atttaaacga gtcaaatcca aaaccaaaaa ggagcttcgt ggcaaatgct ttttctgata
     2941 aaatcgataa tctggaagaa ctggcaataa acaccgcaaa aaaacttgca acaaatcatt
     3001 tattggcttt agggatagat aaagattcaa tagaaaaaag gctcagtaaa tatacaaaaa
     3061 ctccaacacc agaagaatta gaagaagaaa tatgtagctt tagacatcta tcagatcatt
     3121 ttgctgatga gtataaaaat tctgttgata gcaactttat tgaaaattat aacgatagtt
     3181 tctggtctaa acataatcgt gttgtaaaaa ctaaaaaaag tcacttaaaa aagcctttgt
     3241 taaaaccact aatttatgat gacgatataa tttcaagtga tatacccgaa cttgatttgt
     3301 tcttacctgc tgatgaaaat ataactgaaa caacaaaaac agaacaaaaa aacaaagata
     3361 gg
//
LOCUS       DAOSHR010000213         3356 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_26464_length_3356_cov_4.271433, whole genome shotgun sequence.
ACCESSION   DAOSHR010000213 DAOSHR010000000
VERSION     DAOSHR010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 3356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3356
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26464_length_3356_cov_4.271433"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1170)
                     /locus_tag="PLU28_09635"
     CDS             complement(<1..1170)
                     /locus_tag="PLU28_09635"
                     /inference="COORDINATES: protein motif:HMM:NF025841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 domain-containing protein"
                     /protein_id="HOG35358.1"
                     /translation="MKKLGVKALLILFIAFSAVGFSAGENVKPLTGAFFQINGAIANM
                     NYDEMYKELQSMRNIGMDTIIVQYSAYNDVYYYPSKYKETSDELFADESLTNISWNGK
                     VRSRYVKISLEVPAGIEWVITPEIQVMYDSKTLLDGCSYTVNPKPASQYSDANNKLID
                     GKFRYSWEDMVGWQNRKNIEVTFDLKSEKDINFINIMFMNSKVSGVSVPKNGYKVSFS
                     TDGSAFSSAVAVVPDMNKPKDESYGDVIGTILKTADALDMHVYLGLALDSSYWEGKMD
                     PQAQAVYNKNILGELYSMYKSHKSLAGWYLPEEIDDRNFLTKERKDAIVYYLKQMSSY
                     AKMLTKKPVMISPYFGINPDGNAYAIFWEEIFKQAKIDIFAMQDGVGTKRTTAQES"
     gene            complement(1193..2713)
                     /locus_tag="PLU28_09640"
     CDS             complement(1193..2713)
                     /locus_tag="PLU28_09640"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative N-acetylmannosamine-6-phosphate
                     2-epimerase"
                     /protein_id="HOG35359.1"
                     /translation="MILDFKHGLIVSCQAEETEPLYGSEIMQKMAVAAIQGGAKGIRA
                     NTPQDIKAIRQITDLPIIGIFKDRRPDYEAYITITCEEIDEVINAGADIVAIDSIYRS
                     RPEPLEKLYRHCREKYPHIKILADISSVEDVKKILTLKPDYISTTLSGYTAQTCDRSK
                     PDIHLIREITELTDIPVIAEGNYFYPEQVRMAILNGAYAVVVGGAISRPQQTTARFVK
                     HISDLPSSDITAVGVDLGGTWTRSVLTDMYGNILKSYRQETPKNAASVLACIEKCIEK
                     VIDGKTTAIGIASAGRIDFKNGKVLYASDNLPGWTGTEVKKEIEKVFKIRTVVDNDAN
                     MAAYMQWFKEKDGCILYITVGTGIGSGIVVNGKVMRGVTGSAGEIGHIVYPLSSNSCT
                     CGKKGCIETVLSGRYLSENLSDINNPINKKDQRILSEYCKTFAWLVDTLKNTIDFDAV
                     YIGGVLPRYGSFMLESIRREFEKINLRNPGDIIKYSTVGEEAGSVGAALCSLFIDE"
     gene            3123..>3356
                     /locus_tag="PLU28_09645"
     CDS             3123..>3356
                     /locus_tag="PLU28_09645"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOG35360.1"
                     /translation="MLEEKNLSFGVLSDIHSDGIKLKRAIKDLYKIDMKTDALILNGD
                     TVDLGYNREYKKIQKALKSSKNQIPGKIIISLGN"
BASE COUNT         1051 a          670 c          564 g         1071 t
ORIGIN      
        1 cgattcctgc gctgtggttc tttttgtacc aactccgtcc tgcatagcaa aaatatctat
       61 ttttgcctgt ttgaaaattt cttcccaaaa aattgcatag gcatttccat ccggattaat
      121 tccaaaataa ggagagatca taaccggttt tttagtaagc attttagcat aggaactcat
      181 ctgcttaaga taataaacga tagcgtcttt tctttctttg gttaaaaaat tgcggtcatc
      241 aatctcttca ggtagatacc atccggcaag ggatttatgg gatttataca tgctgtaaag
      301 ctcacccaga atatttttgt tataaacggc ctgtgcctgg ggatccattt tcccttccca
      361 gtatgaagaa tctaaagcca atccaagata aacatgcata tctaaagcat cggcagtctt
      421 aagaatagta ccgatcacat caccatagct ctcatccttg ggcttattca tgtcgggaac
      481 aacggcaaca gcagaagaaa aagcagatcc atctgtagaa aaagatactt tataaccgtt
      541 tttcgggaca gaaacacctg atactttgga gttcataaac ataatattta taaaattgat
      601 atccttttca gattttagat caaaagttac ttctatattt tttctgttct gccatccaac
      661 catatcctcc caggagtatc tgaactttcc gtcaataagc ttattatttg catccgaata
      721 ctgggaagca ggcttaggat ttacagtata agaacatcca tcaagaagtg ttttggagtc
      781 atacatgacc tgtatttccg gagtgataac ccattctatg ccggcaggca cttccagaga
      841 gatttttacg tatcttgatc tgaccttgcc gttccagctg atgtttgtaa gcgattcatc
      901 tgcgaaaagc tcgtcggaag tttccttgta cttggatgga taataataga catcgttata
      961 tgcggaatat tgaacaataa tggtatccat tccgatattt ctcatggact gaagttcttt
     1021 gtacatttca tcatagttca tatttgcaat tgctccgtta atttgaaaga aagcaccggt
     1081 aagaggtttt acattttctc cggcagaaaa tccgactgcg gagaaagcta taaataaaat
     1141 caataaagcc tttacaccaa gttttttcat actttcacac tcctaaataa gattattcat
     1201 caataaataa agaacataat gctgcgccta cagaccctgc ttcttctcct acggtactgt
     1261 actttattat atcccccgga tttctcaggt taattttttc aaactccctg cggatgcttt
     1321 caagcataaa gctcccgtaa cgtggtagta caccgcctat atatactgca tcaaaatcaa
     1381 tagtattctt taaggtatca acaagccagg caaatgtttt acaatactcc gataatattc
     1441 tctgatcctt tttgtttatt ggattattga tatcagaaag attttccgac agatacctgc
     1501 ctgaaagaac agtctctata cagccttttt tcccgcacgt acatgagttg gaacttaagg
     1561 gatacacaat gtggccgatt tcccctgctg agcccgttac tcctctcatt acttttccgt
     1621 ttacaacaat tccggaacct atgcccgtac ctacagtgat ataaagtata caaccgtctt
     1681 tttctttgaa ccactgcata tatgcagcca tatttgcgtc gttgtccact actgtacgta
     1741 ttttaaaaac tttttcaatc tcttttttta cttccgttcc tgtccacccc ggcagattgt
     1801 cagaagcata taggacttta ccgtttttaa aatcaattct gccggccgag gcaatgccaa
     1861 ttgccgtagt ttttccatca attacctttt caatgcattt ttctatgcac gccagaacag
     1921 aggcagcgtt tttaggagtc tcctgtctgt aagacttgag aatatttccg tacatatcag
     1981 taagaaccga tctggtccat gttcctccaa gatcaacacc tactgcggtt atgtctgaag
     2041 aaggaagatc gcttatatgc tttacaaatc tggctgtagt ctgctgtggt ctggaaatag
     2101 ctcctccgac aacaactgcg tatgctccgt ttaaaatagc cattcttacc tgttcgggat
     2161 aaaaataatt accttcagcg ataactggaa tatctgttaa ttctgttatt tcacggataa
     2221 gatgtatatc gggttttgat ctgtcacaag tttgtgcagt ataaccagac aatgtagtgg
     2281 aaatatagtc gggtttcaaa gtaagaattt tttttacgtc ctcaacgctt gaaatatcag
     2341 caagaatttt aatatgagga tacttttccc tgcagtgtct gtaaagcttt tcaagaggtt
     2401 ccggtctgct cctgtatatg ctgtctatag ctacaatatc tgctccagca tttattactt
     2461 cgtctatctc ttcgcaagta atggtgatat atgcctcata atccggcctt ctgtctttaa
     2521 atatgcctat tatgggcaga tctgttatct ggcgtatagc tttaatatct tggggagtat
     2581 ttgcccttat gccctttgct cccccctgta tggcagcaac agccattttt tgcattatct
     2641 ccgaaccgta aagaggttca gtttcttctg cctgacagga cactataagt ccatgcttaa
     2701 aatcaagaat catgcgattt cctccaataa aagaaaaagt ctaatgaaat tttacttcaa
     2761 aaaaattcaa aataaaaaaa atattatttc ttatattggt attatataac ataaaggtgc
     2821 ttaaaaaaaa cagactaaaa ttagaaaaat atctgtttat actaaattaa tagccataat
     2881 tggcaataat cagccgagaa taactttgaa aactaaaaac aaccaagata ccgtactgat
     2941 tactataatt ctatgtatac aaaaaaattt ttacaagtat ataagtcaca attcatggta
     3001 tctcagtaag gactaacaac aatgttgatt gtccataaaa caagtagtat aatcgagaag
     3061 aaaaaatatt ttttcataaa gagataaatg tgaagtaaaa tttcaagaaa tgggggtaat
     3121 gtatgcttga agaaaaaaat ctgtctttcg gagtgctgag cgatatacat tcagacggta
     3181 taaaactcaa aagagctatc aaagatttat ataaaattga tatgaaaacg gatgctctca
     3241 tactcaatgg agatacggta gatttaggct ataaccggga gtataaaaaa atacaaaaag
     3301 ctttaaaatc atccaaaaac cagattcctg gaaaaataat catttctctg ggaaac
//
LOCUS       DAOSHR010000214         3342 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_26629_length_3342_cov_4.892911, whole genome shotgun sequence.
ACCESSION   DAOSHR010000214 DAOSHR010000000
VERSION     DAOSHR010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 3342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3342
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26629_length_3342_cov_4.892911"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="PLU28_09650"
     CDS             <1..365
                     /locus_tag="PLU28_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35361.1"
                     /translation="PSYRAPFYLGGEGLAGVTEVGQNLYAVANAGFAFGLNEDYFDII
                     NLDIGATVGVYTESVLEKLTFNIYDYKLYSVLTPKLTLHIFFDRYFSLAITGRYMIDI
                     MQVSPIGYNFSAGFMFGY"
     gene            complement(500..3061)
                     /gene="glgP"
                     /locus_tag="PLU28_09655"
     CDS             complement(500..3061)
                     /gene="glgP"
                     /locus_tag="PLU28_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HOG35362.1"
                     /translation="MEFLKKVTVIPSLPERIKDLKELSMNLYWTWNFNAQKLFENIDP
                     ILWGKTGRNPVTFLKSVSQKELKKATESSDYLELYDNEMKKFSSYKDEKSTWFNTTHK
                     DYTGGKIAYFCAEFGLHESLPIYTGGLGILAGDHVKSASDLGIPFVAIGMIYRQGYFI
                     QKIRSDGTQEDLYEYYNYENFPIQPAKNERNEEIYVTVNILNRTVYIKAWELAVGRVK
                     LYLLDTDIPQNEPEDRHLTYKLYGGDIEMRIKQEIVLGIGGIRMLRKLGINTSVYHMN
                     DSHPNFLVLERIRELIEEKAVTPRQAIEYVRSTSIFTTHTPVPAGNDSFHPNLMERYF
                     KEYVQNLNLTWKEFLDLGKEVRTDGTQMFSMTILALKLAGRANGVSKLHGEVSRNLWK
                     EVWPGLEAVEVPITHVTNGVHSETWIANELQDLYDKYLSPCWRMKQDDPEIWNKIDDI
                     PDEELWAVHKKLKKKLITFTHEKLKEQRMRHGETVEQIQEIEGILDENALTIGFARRF
                     ATYKRALMIFKDLNRLDAILNNPEKPVQIIFAGKAHPADKPGQELIRRINEISRMPNF
                     KNKIVFIEGYDMNVARHLLAGVDVWMNTPRRPHEASGTSGEKAGMNGSINFSVLDGWW
                     VEGYKGNNGWAIGDNRDYTDHELQDKIDSVSIYNTLEKIIVPLYYSGKMDLSYEWTDT
                     IKESIKSVSVFFNTSRMLKEYTQRLYMPAYFQGRKFEDNDFKVSREFSRWREILEKNW
                     NSVKIKVIGTQDITNNLLAGTKVEIQSEIYLPGIGPDSIKVEAVVAKIEGQKVTHLKS
                     YDFRLLKEVSPDTYLYSGEIEFDERGTYGYNIRVAAHHNFMPHRNYIMNLVKYPG"
     gene            complement(3156..>3342)
                     /locus_tag="PLU28_09660"
     CDS             complement(3156..>3342)
                     /locus_tag="PLU28_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35363.1"
                     /translation="KLGRPKTIQLMVLIDRGHREFPIKADYVGKNIPTSKNNEEVKVF
                     IGKESDNPENGVFIYNR"
BASE COUNT          913 a          645 c          591 g         1193 t
ORIGIN      
        1 aaccatcgta tagagctccg ttttatcttg gtggtgaagg attggcgggt gtaaccgaag
       61 tagggcagaa cttgtacgca gtagcaaatg ctggctttgc attcggctta aatgaggatt
      121 actttgatat tataaatttg gatatcggtg caaccgttgg agtatatacc gagtctgttc
      181 ttgaaaaact tacatttaat atctatgact ataagcttta ttcggtactg actcccaagc
      241 ttacgttgca tatatttttt gacaggtatt tttctctggc tattacaggc agatatatga
      301 tagacataat gcaggtatct ccgataggat ataatttcag tgcaggattc atgtttggat
      361 actgattttc ttcacttgat ttcatcggcc ccccgatgat aaataggcag attttatatc
      421 tgcctatttt tgttgataag gattttttat tatctattgt agtaataaca aagtctgccg
      481 gaagttccgg cagactttgt tagccagggt atttaacaag attcattatg taatttctgt
      541 gaggcataaa gttatgatgg gctgcaactc ttatgttata tccgtacgtt cccctttcat
      601 caaactcaat ttcgccagag tataagtatg tatcaggact gacttcttta agaagcctga
      661 aatcatagga ttttaaatga gttaccttct gaccttcaat ttttgcaacc actgcttcaa
      721 ctttaataga atctggtcct attccaggaa gataaatttc tgactgaatc tccaccttag
      781 ttccggctag aaggttgtta gttatatctt gtgttcctat aactttgatt ttgacagaat
      841 tccagttttt ctcaagaatt tctcgccatc ttgaaaactc tcttgatact ttaaagtcat
      901 tatcctcaaa ctttctccct tggaaataag ctggcatata aagcctttgt gtgtattctt
      961 taagcattct tgaagtgttg aagaaaacag aaacactttt tatagattcc tttatggtat
     1021 ctgtccattc gtaggagaga tccattttac cggagtagta aaggggaaca ataatttttt
     1081 ccaaagtatt atatattgat acactgtcaa ttttgtcttg aagttcgtga tcagtatagt
     1141 ctctgttgtc tccgattgcc caaccgttat tgcctttata gccctctacc caccatccgt
     1201 ctaaaacgct gaaatttata gaaccgttca ttccggcttt ttctcctgat gttcctgaag
     1261 cttcatgagg tctacgcgga gtgttcatcc atacgtcgac tcctgcaaga agatgtcgtg
     1321 caacattcat atcgtatcct tctatgaaaa cgatcttgtt tttaaaattt ggcattctgg
     1381 atatttcatt gattctcctt atcaattcct gacctggttt atccgcaggg tgtgccttgc
     1441 ctgcgaagat tatttgcact ggtttttcag gattgttgag tatggcatca agcctgttga
     1501 gatctttaaa aatcataagg gctctcttat aggttgcaaa tcttctggca aagcctatcg
     1561 taagagcatt ttcgtcaaga atgccttcta tttcctgtat ttgttctacg gtttcaccat
     1621 gacgcatcct ctgttcttta agtttttcat gtgtaaaagt aataagcttt tttttaagtt
     1681 ttttatggac agcccaaagt tcttcatccg gtatatcgtc aatcttgttc catatttcgg
     1741 gatcatcctg tttcattctc caacatggag aaagatactt atcatagaga tcctgtagtt
     1801 cattagctat ccatgtttca gaatgaacac cgttagttac gtgagttatt ggaacctcta
     1861 cggcttcaag tcctggccat acttctttcc aaagatttct tgatacttca ccatgaagtt
     1921 ttgaaactcc gtttgctcta ccggcaagtt ttaaagcaag aatagtcatt gaaaacatct
     1981 gtgttccgtc tgtacgtact tcctttccta ggtcaagaaa ctctttccat gtaagattca
     2041 gattttgtac gtattcttta aagtatcttt ccataagatt cggatggaat gaatcgtttc
     2101 cggcaggtac gggagtatgg gtagtaaata tggaagtaga acgtacatac tcaatagcct
     2161 gtcttggagt aaccgctttt tcttcaataa gctcgcgaat tctttccaga accagaaagt
     2221 tcggatgact gtcattcata tgatagacag atgtatttat acctaactta cgtagcattc
     2281 ttataccgcc tattcccagt actatttcct gttttatacg catttcaata tctccgccgt
     2341 aaagcttgta agtaagatgt ctgtcctcag gctcgttttg cggaatatct gtgtcaagaa
     2401 gataaagctt cactcttcct acagcaagct cccatgcttt tatataaact gttctgttta
     2461 aaatatttac agtcacataa atttcttcat tgcgctcatt ttttgccggt tgtataggaa
     2521 aattttcata gttgtagtac tcataaagat cttcctgtgt cccgtcggaa cgtatttttt
     2581 gtataaaata accctgtcgg tatatcattc ctatggcaac aaatggaata cccagatcgc
     2641 tggcggactt tacatgatct cctgcaagga tacccagtcc acctgtgtat atgggaagcg
     2701 attcatgaag tccaaattcg gcacaaaaat aagcgatctt tccgcctgta taatctttgt
     2761 gggtggtatt gaaccatgta cttttttcgt ctttgtatga gctgaatttt ttcatttcgt
     2821 tgtcatacag ttcaagataa tcagatgatt cagtagcctt tttcaactct ttctgagaaa
     2881 cacttttcag aaaagtaacc ggatttctgc ccgtttttcc ccataagata ggatcaatat
     2941 tttcaaaaag tttttgtgca ttgaagttcc atgtccagta caggttcata gacaactctt
     3001 taagatcttt gattctttca ggaagcgatg ggataaccgt tactttttta agaaattcca
     3061 tataaaattc ctcctttaga tttaaaggtt aagaattaca aacagaagta ttatatcaaa
     3121 aaacagggaa aaatcataat atagtatttt aaagactatc tattgtatat aaataccccg
     3181 ttttcaggat tatcagattc ttttcctata aatactttta cttcttcgtt attttttgat
     3241 gtaggaatat ttttacccac ataatcggcc tttataggga actctctatg accacggtct
     3301 ataagaacca taagctgaat agttttaggt cttcccagct tt
//
LOCUS       DAOSHR010000215         3305 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_27040_length_3305_cov_3.725846, whole genome shotgun sequence.
ACCESSION   DAOSHR010000215 DAOSHR010000000
VERSION     DAOSHR010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27040_length_3305_cov_3.725846"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..>3305)
                     /locus_tag="PLU28_09665"
     CDS             complement(133..>3305)
                     /locus_tag="PLU28_09665"
                     /inference="COORDINATES: protein motif:HMM:NF036531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HOG35364.1"
                     /translation="FDFKCTLIKEGGYTFIYKYQESQTEVYAYCSERSSYDEDVEIAD
                     VTLKDRTISFNIKGYNNANAYLYLAGKKLYDNKNEIIDSKRTISLSYPEKIIENSLFI
                     QGFIFSKGKLLRISKEVRIRENKDLSFDFTVNADKNSYKPGEEVTITINSAEDGIFSL
                     SVVDEAIFKMIDRQTDPVKELYGDYFYPEVSIESSSRYYYGYMAKNPDLTQTHKFASY
                     KGAGEIKNNTREYFPDTALWLPTVKTTDGKAVIKFKNPDSVTSWRITAEGISNYKLAS
                     QKSNFISTKNFYARPLLPNFCYRGDNFSFPVVVYNNTEEELFFVYKAMIDNPGLSVTP
                     TNGGVIVGANSSKVLNFNVDALNFGNFNFTFHFDSWKVSDSIKLPFEVKLNQLKTDIR
                     TIQYVQDGDTLKKGVQARKVNLEKIIRENIEYLISYPYGCTEQTMSSFYPALMSKSLN
                     LEIENTQDIILKSVQRLYKYQHDDGGWGWWMNDLSDPGISAYVMEGLLRLKNTGYYLP
                     QTVLKNALSYLKSNILDGYIYHVLKLWGEAPEDFVPTSLYDKLFFAYHDDTTYNEVLS
                     MMQTHSEAYFINYSDESYYHTQIEMNAILLDLLLTKNAQEDKVIGMVKYLLANKKSLF
                     WNSTKDSARTLLALIKFIQNYSDSFSGDVSLTDEFDFILSDTLIKGNGLIETKKTEYL
                     APVKNNSGIDIVRSIFKRYELPVSFNSNDYIADAFIPIESNKVPSKLEIISLDDFYRD
                     RSADTLIWNISSTKPGTFIEHMGKTVSYTEEMNFTINGIQFPYPQRFEIISDKGFIYS
                     YDSLICYNFTDSSTCLISDSAIKDYKIYNGKKYILQEDSEKKQDTLMIEDRFYQFGKS
                     FQAIDIFEDKVFLHGDGLYLFDESSQEKLKKIFPVSGQKILSLDENSITLYGNIRFEG
                     NSSYAGKTGIYKITFKTEKPSLYKGDILKTQIFFKTEIPSYIAVEDYLSGSTQVLTNY
                     SEKVINTSNKFDYYWYKPWDNWYSSKEIRKDRVAFFQNGFETGSFEYYSRLTHSGLFR
                     IMPAQAYSMYWITNFGTSDSIEMNVK"
BASE COUNT         1019 a          574 c          542 g         1170 t
ORIGIN      
        1 aaagtaaata tcctcttttt cattgtcgtt cctcctttta aataagttca ggaagattat
       61 acaacattaa agccaagtaa aaaagctgtt cttgacaaac tgcttttaaa acagaataag
      121 atgtcaaaac tattatttta cattcatttc tatagaatct gatgtaccaa agttggttat
      181 ccagtacatc gaataagcct gagccggcat aatacgaaat aagccactgt gagttaatcg
      241 tgaatagtat tcaaaacttc cggtttcaaa accattttgg aaaaaggcca ctctgtcttt
      301 tcttatttct tttgagctat accagttatc ccaaggctta taccaatagt aatcgaattt
      361 atttgatgta tttattactt tttctgaata attcgtaagc acttgtgtgc ttccgctcaa
      421 ataatcctca acagcaatat aagatgggat ttcagtttta aaaaagattt gagtttttaa
      481 aatatctccc ttatacaaag acggtttctc tgttttgaaa gttattttat agataccagt
      541 ttttcctgca tagctcgaat taccttcaaa tcttatattt ccgtaaagcg taatagaatt
      601 ttcatctagt gaaaggatct tctgaccgga gacaggaaag atttttttaa gtttttcctg
      661 ggaagattca tcaaatagat ataggccatc gccatgtaaa aatactttgt cttcaaatat
      721 atcaattgcc tgaaaagact ttccaaactg gtaaaaacga tcttctatca taagagtatc
      781 ctgttttttc tctgaatctt cctgaagtat atatttttta ccgttgtata ttttataatc
      841 ttttatagct gagtcggata taagacaagt gctggaatca gtgaaattat aacatattag
      901 ggaatcataa gagtatataa atcctttgtc tgaaataatt tcaaaacgtt gaggatacgg
      961 aaactggatt ccgtttatgg taaagttcat ttcttctgta taggatacag tttttcccat
     1021 atgttctatg aaagttcctg gcttagtgga gctgatattc catataagag tatctgcact
     1081 tctgtctctg taaaaatcat caagagaaat aatctccagt ttggaaggaa ctttattgct
     1141 ttcgattggt atgaaagcgt cggcaatata atcattgctg ttgaatgaaa ccgggagttc
     1201 gtatcgtttg aagatacttc gcactatatc tattccacta ttgtttttca caggagcaag
     1261 atactcagtt tttttggttt ctattaatcc gtttcctttt atcaaagtat ctgaaagtat
     1321 gaaatcaaat tcatctgtta aggagacatc ccctgaaaaa ctgtcagagt agttttgaat
     1381 aaattttata agagccagaa gtgttcttgc actgtccttt gtcgagttcc agaaaagaga
     1441 ttttttattt gcaagaaggt actttaccat tccgatgact ttatcctctt gagcattttt
     1501 ggtcagcagc agatccaaga gtatagcatt catctcaatt tgtgtatgat agtaactctc
     1561 atcagagtaa ttaataaaat aagcttctga atgagtctgc atcatcgaaa gcacttcatt
     1621 ataggtagta tcatcatgat aagcgaaaaa caatttatcg tataatgaag tcggaacgaa
     1681 gtcctcaggg gcttcacccc agagttttaa tacatgatat atatatccat cgagaatatt
     1741 tgattttaaa taagacaaag cgtttttaag aactgtctga ggcagataat atccggtatt
     1801 tttcagcctt aaaagacctt ccattacata tgcacttata cctggatccg aaagatcgtt
     1861 catccaccat ccccatccgc catcatcatg ctgatattta taaagccgtt gtactgattt
     1921 tagtattata tcctgagtat tttcaatttc caagttaagt gattttgaca tcagtgcagg
     1981 ataaaaggat gacatagtct gttctgtgca tccgtaagga taagaaataa gatactcaat
     2041 attttctctt ataatctttt ctagattcac ttttcttgcc tgcacaccct tttttaaagt
     2101 atctccatcc tgaacatact gaatggttct tatatcggtc ttaagctggt ttagctttac
     2161 ttcaaaaggc agcttgatac tatcagacac tttccatgaa tcaaagtgaa aagtaaaatt
     2221 aaaatttcca aagttcaagg catctacatt aaaattaaga actttagaag agtttgcacc
     2281 tacaataact ccaccattag tgggagtaac tgaaagtcct ggattgtcaa tcatagcttt
     2341 ataaacaaag aaaagttctt cttctgtgtt attgtaaaca actaccggaa aggagaagtt
     2401 atctcctctg tagcaaaagt ttggaagaag cggtctggca taaaaatttt tcgttgatat
     2461 gaagttactt ttttgggatg cgagtttgta attgcttatt ccttctgctg ttatccgcca
     2521 tgaagttact gaatcaggat ttttaaattt aataaccgct ttaccatcag ttgttttgac
     2581 tgttggaagc caaagcgcgg tatcaggaaa gtactctctg gtattgttct taatctcacc
     2641 ggctcctttg tatgatgcaa atttatgagt ctgtgtaaga tcgggatttt ttgccatgta
     2701 tccgtagtag tatcttgaag aactttctat tgaaacctct ggataaaaat aatcaccgta
     2761 taattcttta acaggatcag tctgcctatc tatcatttta aatatagctt cgtctaccac
     2821 acttaatgaa aaaataccat cctctgcaga attaatggtt atggttacct cttcgccggg
     2881 cttataagag tttttgtctg cattcactgt aaagtcaaaa gaaagatctt tattttcgcg
     2941 tattcttact tctttgctta tccttagaag ttttcccttt gagaagataa agccttgtat
     3001 aaaaagcgag ttttctatta ttttttccgg atacgaaagc gaaatagtac gtttgctatc
     3061 aattatttca tttttattat cataaagctt ttttcctgca agatacaaat aggcatttgc
     3121 gttattgtat ccttttatgt taaaggaaat tgttctatcc tttaatgtaa cgtctgcaat
     3181 ttcaacgtct tcatcatagc tgcttctttc actgcagtaa gcatatactt ccgtttgact
     3241 ttcctgatac ttgtagataa aagtatatcc gccttctttg ataagtgtac atttgaagtc
     3301 aaaag
//
LOCUS       DAOSHR010000216         3304 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_27056_length_3304_cov_6.409357, whole genome shotgun sequence.
ACCESSION   DAOSHR010000216 DAOSHR010000000
VERSION     DAOSHR010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 3304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3304
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27056_length_3304_cov_6.409357"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="PLU28_09670"
     CDS             complement(<1..469)
                     /locus_tag="PLU28_09670"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOG35365.1"
                     /translation="MKKAITVMVLVLLILAVPVKSQEILQDQNIEMVLVEKGSFEMGD
                     TFEIVCLAVNNVHTATFTYDYYIGKYEVTFDQYDRYCQETKKATPDDNGWGRGSRPVI
                     NVSWYDAIAYCNWLSEKEGLAKAYDSEGKFLDSSGKITTDPSLVEGYRLPTEAE"
     gene            complement(757..1236)
                     /locus_tag="PLU28_09675"
     CDS             complement(757..1236)
                     /locus_tag="PLU28_09675"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HOG35366.1"
                     /translation="MFVKFQKRESFKVAGINVKTSMKTASVDCPSAWDKFNPFCQSME
                     SLKNKNEYFGICNAYNQKEGTFSYMACTTVNENDDVKGLDTLKIPAANYAVFKHQGAL
                     CDISKTYDEIFQKWMPSSGYMYDPNGNDFELYNEEFDCKEEKNCILYICIPVVKASK"
     gene            1358..2272
                     /locus_tag="PLU28_09680"
     CDS             1358..2272
                     /locus_tag="PLU28_09680"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HOG35367.1"
                     /translation="MGNFHRIQWIDGAIKEKRYPCIKTISERFEISDRQVYRDIEYMK
                     YSLGAPIEYSAKEKGYLYTDSTFTLPSFFISEEEKAALKFLSVQYSKIKEESSQNLAR
                     LFSRLSGQDDENISQISIPFYQFSVGNAKNFSTVQSAISQDKKLDMSYVNYQGIFQQR
                     TVSPYKIFKNGDRYYFVGYCHLKQDLRVFRLDRAGSMAVREEKRYKCPYYDPEKYVDD
                     MTYFEYIRPYKCTIEFCEEMDEQNIKFKVTSKNGKRYGIEFFSSYDLLGWLFTVSSKF
                     KIIEPVWLKEKLKQKIKKIYSMNEGEPL"
     gene            2269..3237
                     /locus_tag="PLU28_09685"
     CDS             2269..3237
                     /locus_tag="PLU28_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35368.1"
                     /translation="MSSKVLEGVKRVGLWNSFMGSVKAVLEYTNMKNIEDYMLMGSTG
                     MAFRFVMKSNCDASGPTVYDWASEHFTMLDRIGIFSEITLLFREQGMNTFKIAQASAM
                     EKIKSAIDRNTPVIIWAPSSIMEFGIICGYDDEQRVFFVRDVGEDEPDPLLYDNLGIS
                     MIPYLYVHVLESCTDVPQEKMFRSSLVYGIKEWNKKFHVSSSYTCGNAAYQSTIDSLS
                     SERFNDFGFCYCMAVYAQSKELLAEYIKELSIRSKEIKIPSKTVEYCMDISDGFKEIS
                     MKTPFLGPKKVHKVDCKKELALYLKQLREKEEYVMKSLEKAIGSAN"
BASE COUNT         1011 a          560 c          679 g         1054 t
ORIGIN      
        1 actctgcttc tgtaggaaga cggtatcctt cgaccagact tggatctgtg gttatctttc
       61 cgcttgagtc aagaaacttt ccctcactgt catatgcctt tgccaaccct tctttttcac
      121 tcagccagtt acagtaggct atggcatcgt accagcttac attaattaca ggtctgctgc
      181 ctcttcccca cccattgtca tccggtgtgg cttttttggt ctcttgacag tacctgtcat
      241 actggtcaaa ggttacttca tacttgccta tataatagtc gtatgtgaag gttgccgtat
      301 gtacgttatt tacagctaaa catacaattt caaatgtatc tcccatttca aagcttcctt
      361 tttccaccag aaccatttcg atgttttgat cttgcaatat ctcctgactt tttaccggta
      421 ctgccagtat aagaagtaca agaaccatca ctgtaatagc tttcttcatt tcagctctcc
      481 tttcatactt ttgatttaac aattattttc tatatactcg tcgttttatt ttttataaca
      541 atccactata ataaatctta ccatattttt gtcattaact gtcaagctac aaacaacaag
      601 tttttcagat ttttgtggag tacaagggat atgaaactta tggaacggta ggagtaaatt
      661 atctatacgg atacagaggc aaagaaaagg actgttattg agtagtagga tcatgtaata
      721 agaagaccgg ggttcataac cccggtcttt gttctgttat tttgaagctt ttacaactgg
      781 tatacatata taaagtatgc agttcttttc ttctttacag tcaaactcct cgttgtaaag
      841 ttcaaaatca ttaccgttgg gatcgtacat gtatcctgag gaaggcatcc atttctgaaa
      901 tatttcatca taggttttgg aaatatcaca aagggcgccc tggtgtttaa aaacagcata
      961 gtttgctgcc ggtattttca gtgtatccag acctttcaca tcatcatttt cattaaccgt
     1021 ggtgcatgcc atgtagctga aggtgccttc cttctggttg taagcgttgc atatgccaaa
     1081 gtattcgttt ttgtttttaa gactttccat actctggcag aaaggattaa acttgtccca
     1141 cgcacttggg cagtctacag aagcggtttt catacttgtt tttacattga tgccggcgac
     1201 cttgaaactt tctctttttt ggaattttac gaacatacca tcacctccgt tatttttgtt
     1261 cactgatttt attatagcgt atagggactg acacagtctg tcagcatgaa tatgtgttat
     1321 aattatggta tatctttata taattaagaa gtgggatatg ggcaattttc ataggataca
     1381 gtggatagac ggtgcgataa aagaaaaaag atatccttgc ataaaaacga tatccgagag
     1441 gtttgagatt tcagacagac aggtctacag ggatattgaa tatatgaaat actctctggg
     1501 tgcacctata gagtactctg caaaagaaaa agggtatctg tatacggata gtacattcac
     1561 acttccttct ttttttataa gcgaggaaga aaaagccgca cttaagtttt tgagtgttca
     1621 atactcaaaa ataaaagagg aaagcagtca aaatcttgcc aggctttttt cacgtctttc
     1681 ggggcaggat gatgaaaaca tttcccaaat aagcattccg ttttatcagt tcagtgtggg
     1741 gaacgcaaaa aactttagta ctgttcagtc ggctatttct caggataaaa aattagatat
     1801 gtcgtatgtg aattatcagg gaatttttca gcagagaaca gtttctccct ataaaatatt
     1861 taagaacggt gacagatatt attttgtagg atactgtcat cttaagcagg atcttcgtgt
     1921 attcaggctt gacagggcag gcagtatggc agtaagagaa gaaaaaaggt ataaatgtcc
     1981 ttactatgat ccggaaaagt atgtagatga catgacttac tttgaatata taagaccgta
     2041 taaatgtacc atagaatttt gtgaagagat ggatgagcaa aacataaagt ttaaagtaac
     2101 ttccaaaaat ggaaaaagat acggtataga gtttttcagt tcatatgatc ttttgggatg
     2161 gctttttacg gtttcatcaa agttcaagat catagagcct gtatggctga aagaaaagct
     2221 gaaacagaaa atcaaaaaaa tatacagtat gaatgaaggt gagccgctat gagttcaaag
     2281 gttcttgaag gagttaaaag ggtcggtctg tggaattctt ttatgggaag tgtcaaagct
     2341 gttcttgagt atacgaatat gaaaaatatc gaagattata tgcttatggg ctcgacagga
     2401 atggcgttca gatttgttat gaagagtaat tgtgatgcca gcgggccaac tgtttatgac
     2461 tgggcgagcg aacattttac gatgctcgac agaataggta tattttcaga gataacgctt
     2521 cttttcagag aacagggaat gaacactttc aagatagcgc aggccagtgc tatggaaaaa
     2581 ataaaaagtg ctatagatag aaatactcct gttataatat gggctccaag cagcattatg
     2641 gagtttggta ttatatgtgg ttatgatgat gaacagcgag tattttttgt cagagatgtg
     2701 ggtgaggatg aacctgatcc attgctttat gataatctgg gtatttccat gattccatat
     2761 ctttatgtgc atgtgcttga aagctgtact gatgtgccgc aggaaaaaat gttcagaagc
     2821 tctttggtat acggtataaa agaatggaac aaaaaatttc atgtctcttc atcttatacg
     2881 tgtggaaatg cggcgtatca aagtaccata gattctcttt cttctgaaag gtttaatgat
     2941 ttcggattct gttactgcat ggcagtatac gcacagtcta aagagctttt ggcggaatat
     3001 ataaaagagc tttccataag atcaaaggag ataaagattc cgtccaaaac ggtagagtac
     3061 tgtatggata tatccgacgg attcaaggag atcagtatga aaacaccttt ccttggtccc
     3121 aaaaaggttc ataaggttga ttgtaaaaaa gagcttgctt tatatctcaa gcagttgaga
     3181 gaaaaggaag agtatgttat gaaatcactc gaaaaagcaa ttggaagtgc taattaggtg
     3241 atactcctgc cgcagaaaac attttgatgg cgagttccgt attctcgtat acgcctgtaa
     3301 aaag
//
LOCUS       DAOSHR010000217         3292 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_27198_length_3292_cov_5.008959, whole genome shotgun sequence.
ACCESSION   DAOSHR010000217 DAOSHR010000000
VERSION     DAOSHR010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 3292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3292
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27198_length_3292_cov_5.008959"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="PLU28_09690"
     CDS             <1..295
                     /locus_tag="PLU28_09690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35369.1"
                     /translation="DIAKDPRVAGCYKIHQIWGKGVNMGNPKKPENLEFASTLRKVFF
                     KFYDRHVNEDDPECCILRIIISNAVVFSPDAKYIVDFHNRSAQKIAMKLDIIE"
     gene            448..1029
                     /locus_tag="PLU28_09695"
     CDS             448..1029
                     /locus_tag="PLU28_09695"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOG35370.1"
                     /translation="MQDKKELIYNCARKIFGSKGFKDTAISDIAQSAGISVGTFYNYY
                     SSKENLFMEIFLHENVKLKKECFEKLDKSMSPMDTVKQMMELNFQGMKANTILSEWYN
                     KAVFEKIEKTYRQENGVNAVDFLYGDFLKIVEQWQAQGKMRADIDSRMIMMIFASIIN
                     IDTHKQEIGLEYFPQLVHIITELIMQSLTPPQK"
     gene            1078..2037
                     /locus_tag="PLU28_09700"
     CDS             1078..2037
                     /locus_tag="PLU28_09700"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOG35371.1"
                     /translation="MVYAMDTEVTCKIFSDERSRESDLVTNELKKLEKKLSRFVKKSE
                     VCKINSNAGKKPVKVSRDTFELLAESKRYSELSGGLFDISIAPLMNLWDYKNSLSVPE
                     NDRIEKTVSLVNYMDLILNERKQTVMLRKPGQAIDLGAIGKGFAADRCTALLKNKGID
                     SAFISIGGSVSLIGRKPGGSLWSVGIRHPRKTGELIGVLQSESKHIVTSGDYERFFID
                     KNGRCWHHILNPKTGYPMNSGIASVTVVAHSAVMADALSTIIFLSGINEVQRYIEQFT
                     DIQIIIIDEKLNIYITQGLKDFFSSVQNSEICIIGGRKDDEKN"
     gene            2024..2479
                     /locus_tag="PLU28_09705"
     CDS             2024..2479
                     /locus_tag="PLU28_09705"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HOG35372.1"
                     /translation="MKKTKKRKGKPKMLITVAVIIGVVVVGLTAGILWSLPGMREIEG
                     MKIESIDFKEFKNGVYTGKYIGSKDHLRDTELQVSIEEGKISDIKVIKGALDKNGQPV
                     KLTGGKTVEDLFDRVLSSQSLQVDVISGATLTSKTHLKALEDALKKAQE"
     gene            2676..>3292
                     /locus_tag="PLU28_09710"
     CDS             2676..>3292
                     /locus_tag="PLU28_09710"
                     /inference="COORDINATES: protein motif:HMM:NF019976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="HOG35373.1"
                     /translation="MIVYNNNKATPKKDQPTEGEVELLNFLENNLDDSYEIYYKPWIN
                     DLHPDIMIMKKDTGVIVIEVKDWDLNDYFVKKENEKVSWYLKKDSMKVLSPFEQVKKI
                     KDCFYEGEIKDLRSKKAKNQSFYGIVKTLVYFKNVTKDELISFGQGCLAFKNNLVCEG
                     GDKYRNAITQDMLNASYLEKILNSLHFKLNKLFDKELYESFREKVL"
BASE COUNT         1213 a          472 c          691 g          916 t
ORIGIN      
        1 agatattgcc aaagatccaa gggttgcagg gtgttataaa attcatcaga tctggggtaa
       61 aggtgtgaat atgggcaatc ctaaaaagcc cgaaaatttg gagtttgcca gcactctaag
      121 aaaggttttt ttcaaattct atgacagaca tgtgaatgaa gatgaccctg aatgttgtat
      181 cctaagaatc attatttcaa atgctgtggt tttcagcccg gatgcaaagt atatagttga
      241 ttttcacaac aggtcagccc aaaagatagc tatgaagctt gacataatag aataaacatc
      301 ctttcttaaa aagaaaggat gttctttata tatatagcaa agtttttaaa ttaaagctta
      361 ggtacattca aaaaattgac aaaataaaaa cattatgata ctatatgaat gaataaaaaa
      421 atattcattc attttttgga ggaaagtatg caggacaaaa aggaactaat atataactgt
      481 gcaagaaaaa tatttggcag caagggtttt aaggatacgg ccatttctga tatagcacaa
      541 agtgcgggta tttctgtcgg aactttttat aattactact cttcaaaaga aaatcttttt
      601 atggaaattt ttttacatga aaatgtcaaa ctaaaaaagg aatgttttga aaaacttgat
      661 aaatccatga gtcctatgga cactgtaaaa caaatgatgg agcttaattt tcagggcatg
      721 aaagccaata cgatactttc ggagtggtac aacaaagctg tttttgaaaa aattgaaaaa
      781 acatataggc aagaaaacgg agtgaatgct gttgattttc tctacggaga ttttctgaag
      841 atagtagaac aatggcaggc gcagggaaaa atgcgtgcgg atatagacag caggatgata
      901 atgatgatct ttgcctcaat aataaatatt gatacgcata agcaagaaat aggcctggaa
      961 tactttccgc agttggttca tatcataacg gagcttataa tgcaaagctt aactccacca
     1021 caaaaataga tatacggacg gcatataagt atacagacta caaaacaggg agataaaatg
     1081 gtgtatgcca tggacacgga agtaacctgc aagatatttt ctgatgaaag atcacgggaa
     1141 agtgaccttg tgactaatga attgaaaaaa ctggaaaaaa aactcagtcg gtttgtaaag
     1201 aaaagcgaag tatgtaaaat taacagcaat gccggaaaaa aacccgtaaa agtaagcagg
     1261 gatacttttg agcttcttgc agaatctaaa agatattcag agctttcagg cggacttttt
     1321 gatatttcta ttgctccgtt gatgaacctt tgggactata aaaattcttt gtctgttccc
     1381 gaaaatgata ggatagaaaa gactgtttct ttagtaaact atatggattt gattctgaat
     1441 gaaagaaaac agacggtgat gctcagaaag cccggacaag caattgatct tggagcaata
     1501 ggaaaagggt ttgctgcgga tagatgtaca gcgcttttaa aaaataaggg aatagattct
     1561 gcatttataa gtattggcgg gagcgtatcc cttataggca gaaaacctgg aggttcttta
     1621 tggagtgttg gaataaggca tccacgtaaa accggggagc ttataggagt gctccaatca
     1681 gaaagtaagc atatcgtgac ttccggcgat tatgaaaggt tttttattga taagaacgga
     1741 aggtgctggc accatatact gaatcctaaa acaggctacc ccatgaactc aggtatagca
     1801 agcgtaacag ttgtcgccca cagtgctgtt atggctgatg cactttcaac cataatattt
     1861 ctttcaggga ttaatgaagt acagagatat attgagcagt tcacagatat tcaaataatt
     1921 ataattgatg aaaaattaaa tatttatatt actcagggat tgaaagattt ttttagctct
     1981 gttcaaaatt ctgaaatatg tattatagga ggaaggaagg acgatgaaaa aaactaaaaa
     2041 aaggaagggg aaaccaaaaa tgcttattac ggtagcggtt attatcggag tagttgtagt
     2101 cgggctaaca gctggaatat tatggagtct tcccgggatg cgcgagatag agggaatgaa
     2161 aatagaaagc atcgatttta aggagttcaa aaatggagta tacactggga aatacatagg
     2221 aagcaaagat catttgaggg ataccgaatt acaggttagt atagaagaag gaaaaatttc
     2281 tgatattaag gtaataaaag gagcattgga taagaacggc cagcctgtta agctgacggg
     2341 cggtaaaaca gttgaggatc tttttgatcg ggtattgagt tcacagtcac ttcaggtgga
     2401 tgttataagt ggagcaacgc ttacatctaa aacacatctt aaagcgctgg aagatgcact
     2461 caaaaaagct caggaataaa aaatactctc cgttatgaag cgatcccaaa gtttagacaa
     2521 aattgaatat taaaccattt gtgaatgagt tcggtattgt accgggctca ttcttttttt
     2581 attaagcatt ggtagtatgg cgcatatagc aatcatttct aaatgtcgaa taataatttg
     2641 gaagaacgca taaaattctg acggaggtga atagtatgat cgtttataac aacaataagg
     2701 ctacccccaa aaaagatcaa cctaccgaag gagaagtcga gctcttaaat ttcttagaaa
     2761 acaatctgga tgattcttat gaaatatact ataaaccctg gataaatgat ttacaccctg
     2821 acataatgat aatgaaaaag gatactggag ttattgttat agaggttaaa gattgggatc
     2881 taaacgatta ctttgtaaaa aaagaaaacg aaaaagtatc atggtatctt aagaaggatt
     2941 ccatgaaggt gctttcgccg tttgaacaag ttaagaagat taaagactgc ttttatgaag
     3001 gcgaaattaa agatcttaga agcaaaaaag ctaaaaatca atccttctac ggtatagtaa
     3061 aaactcttgt ttactttaaa aacgtcacta aggatgagct tataagcttc ggacaagggt
     3121 gtcttgcttt taaaaacaat cttgtttgcg aaggcgggga caagtataga aacgctatta
     3181 cacaagatat gttaaacgca tcgtatcttg aaaaaatctt aaatagtctg cactttaaat
     3241 tgaacaagct ttttgataaa gaactttatg aaagcttcag agaaaaagtt ct
//
LOCUS       DAOSHR010000218         3285 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_27293_length_3285_cov_4.119814, whole genome shotgun sequence.
ACCESSION   DAOSHR010000218 DAOSHR010000000
VERSION     DAOSHR010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27293_length_3285_cov_4.119814"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="PLU28_09715"
     CDS             complement(<1..442)
                     /locus_tag="PLU28_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35374.1"
                     /translation="MIDRVYAYKRIQDFFPQSKGSDIIFNEEGSVNLIAFLNNERVFK
                     FPREEWGEIYLKREVILSKNIARISSINVPEFDIVENDFGSYKMIHGTDLSSESIKRL
                     SVAEQKNIAWELALFLKTINELKIDGPWFSDSYRSKEDWIKLYED"
     gene            complement(534..1199)
                     /locus_tag="PLU28_09720"
     CDS             complement(534..1199)
                     /locus_tag="PLU28_09720"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOG35375.1"
                     /translation="MYEKTEAFWDDIFGKEELKDLSKPFGIEKMDSCIGWLCANTGSI
                     IDYGCGNASMLLRCAYLNVKKGLGIDISCQAVRTAVDYISKKDLSGFSVIQGSLEALK
                     SIKDASFEGAILSNIIDNILPDDALCVLKNIYRILTHGGRVFVKLNDFMDKEKLKESI
                     DFFEKEISDNFFLEKSGLYFHNLSDEKFERWIMEYFKVVEKGRIEFKTFDCFNRFYLL
                     QKL"
     gene            complement(1394..2218)
                     /locus_tag="PLU28_09725"
     CDS             complement(1394..2218)
                     /locus_tag="PLU28_09725"
                     /inference="COORDINATES: protein motif:HMM:NF014049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF92 domain-containing protein"
                     /protein_id="HOG35376.1"
                     /translation="MLNFVIGLVLSFIISFYAYRKKSLTISGVVGAMALGSSLYFFGG
                     FLFFAILIAFFISSSLLTKFKKSEKKDTEIITEKSGKRDYSQVIANGLPAFLTAFLYY
                     ITQNHVFMLGFAVSMASSNSDTWASELGVLSKNQPVSILTWKRIKKGMSGGISLMGTL
                     ASLLGSLVISLIFTVGYALKYETVITFKNIAFVFTVCFFGGFIGSIIDSLLGASVQAL
                     YYCEKLKIYTERRFFAESENKLVKGIKFMNNNMVNFLSGLLASLLSMVLFILTVNI"
     gene            complement(2232..2888)
                     /locus_tag="PLU28_09730"
     CDS             complement(2232..2888)
                     /locus_tag="PLU28_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35377.1"
                     /translation="MNFIGILASIILVFLIIGISGLLEKKKILSKEGSRKFVHIGVSN
                     WWILAMMFFDSNIWAAVVPALFIIINYISYKKNIFKSMERGAGKKDLGTVYFAVSLFV
                     LSLMTFTKSSSPFIGAMGILIMGYGDGFAAIIGSKYGKHSFKIIDSTKSLEGSAAMFV
                     FSFIVSLIIMLIHADQNFLVYSFIIALVAMFTELFSVFGLDNLTVPLITSYLYYLLSF
                     "
     gene            complement(2885..>3285)
                     /locus_tag="PLU28_09735"
     CDS             complement(2885..>3285)
                     /locus_tag="PLU28_09735"
                     /inference="COORDINATES: protein motif:HMM:NF012705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HOG35378.1"
                     /translation="AELGKAMQITNILRDIGEDFAMKRVYLPLKVMKMFKYSVDDLSK
                     STINRNFKNLWDYEAKIAEKYYAKFLSAVDYLDEKSKKPVLASALLYQKILDKVRENG
                     YDCFGSRNYVTQDEKKIIISSIFLREERRV"
BASE COUNT         1075 a          631 c          516 g         1063 t
ORIGIN      
        1 catcctcata taactttatc caatcttctt ttgaccggta tgaatccgaa aaccacgggc
       61 cgtctatttt tagttcgttt atggttttta aaaataaagc aagctcccaa gcaatatttt
      121 tctgttcagc aacggaaagt ctttttattg attctgaact caaatcggta ccatgaatca
      181 ttttgtaaga accaaaatca ttttcgacaa tatcaaactc cggaacattg attgaagata
      241 tccgggcaat attttttgat aagattacct ctcttttaag ataaatttct ccccattctt
      301 cacgcgggaa cttaaaaact ctctcattgt taagaaaggc tataagattt acgcttcctt
      361 cttcattaaa gataatatcg cttccttttg actgtgggaa aaaatcttgt attcttttat
      421 atgcgtatac tctgtcaatc atttttttcc ctcctataat tatatctgtg gataagtaaa
      481 tgatactaca ttttataaaa taatgctata tgcacagcga tatattaatt tttttaaagt
      541 ttttgcaata aataaaatct attgaagcaa tcaaaagtct tgaactctat ccttcctttt
      601 tctacaactt taaagtattc catgatccat cgttcaaact tttcatcaga aagattatga
      661 aagtaaaggc cgcttttttc aaggaaaaaa ttatctgaaa tttctttctc gaaaaaatca
      721 atactttctt ttagcttttc tttatccata aaatcattaa gctttacaaa aactcttcct
      781 ccatgcgtaa gtatcctgta tatattttta agaacacaga gtgcatcatc tggaagaatg
      841 ttgtcaataa tatttgaaag tatggcgcct tcaaaggaag catctttgat gcttttcaaa
      901 gcttccagac ttccctgtat cacagaaaaa cccgaaaggt ctttttttga aatataatca
      961 acggcagttc ttacagcctg gcagcttatg tctattccta accctttttt tacattgaga
     1021 tatgcacatc ttagaagcat agaggcattt ccgcatccat agtctataat gcttcccgta
     1081 ttggcgcaaa gccagcctat acagctatcc attttttcaa ttccaaacgg ttttgaaagg
     1141 tcttttaatt cttcttttcc aaaaatatca tcccagaagg cttctgtttt ttcatacact
     1201 gttgtccacc tctttttact ttttttgttc tgacccaaag aaaccggcaa gctgttgatg
     1261 ggtattgatt ttgaatagca aaaagacttt ccactgcctg cataggataa tcaacaggtg
     1321 cggaaagctt ctgtttataa aaaataatct ggtttaattt tgaagtgctt attaacatag
     1381 ttcctccaaa tacttatata ttaactgtca atatgaacag aaccattgat aacaacgaag
     1441 cgagcagtcc gctcagaaag ttgaccatgt tgttattcat gaattttata ccttttacaa
     1501 gcttgttttc actttcggca aagaatctcc tttcggtata tatcttaagt ttttcgcagt
     1561 aataaagagc ctgaacggat gcccctagaa ggctgtcaat tatagagcct ataaatcctc
     1621 cgaaaaaaca tacggtaaat acaaaggcta tgtttttgaa agttatgaca gtctcatact
     1681 tcagagcata tccgactgta aaaataagag atataaccaa ggagcccagt aaagaagcca
     1741 aagttcccat aaggcttatt cctccggaca ttcctttttt aattcttttc catgtaagga
     1801 tggagacagg ctgatttttg ctcagaaccc ccaactctga agcccacgtg tctgagtttg
     1861 agcttgccat tgaaaccgca aaaccaagca taaaaacatg attctgggtt atatagtata
     1921 aaaaagctgt cagaaatgcc gggagaccgt tggcaataac ctgagaatag tctcgctttc
     1981 cggatttttc tgtaataatt tctgtatctt ttttttcaga ttttttaaat ttggtcagaa
     2041 gactcgagga tataaaaaaa gcaataagta tggcaaaaaa aagaaagccg ccgaaaaagt
     2101 agaggcttga gccaagagcc attgctccga caacgccgct tatggtaagt gatttttttc
     2161 tgtaagcata aaaagaaata ataaaactta aaaccaaacc aataacaaaa ttaagcatga
     2221 gacactctcc ttcagaaaga aagcagataa tataagtaag aggttataag cggtacagta
     2281 agattatcaa gaccaaatac ggaaaataat tctgtgaaca tagctaccaa agcaattatg
     2341 aaagagtaga ctaaaaagtt ctgatctgcg tggataagca ttattataag cgatacaatg
     2401 aaagagaaaa caaacattgc cgcacttccc tcaaggcttt tggtgctatc aataatttta
     2461 aaactatgtt ttccgtactt gctgcctatt atggctgcaa agccatctcc gtatcccata
     2521 atcagtatac ccattgcacc gatgaaaggg gaagaacttt tagtaaaggt cataagggaa
     2581 agcacaaaaa gagaaactgc aaaataaacc gttccaaggt ctttctttcc ggcacctcgt
     2641 tccatagatt taaaaatatt ctttttgtag gaaatgtagt taatgataat gaaaagtgcc
     2701 gggacaactg ctgcccagat gttactgtca aaaaacatca tagccagaat ccaccagttt
     2761 gatacaccta tatgcacaaa ctttcgggaa ccttccttgg aaaggatctt ctttttttca
     2821 agcaatccgg aaatgcctat aataagaaaa actaaaatta tagaagccaa aattccgata
     2881 aagttcatac tcttctctcc tctcttaaga aaatgctgct tataatgatc ttcttttcat
     2941 cttgagtcac gtaatttctt gatccaaaac aatcataacc attttctcta actttatcaa
     3001 ggattttttg atataaaaga gctgaggcca gtaccggttt ttttgatttt tcatccagat
     3061 agtctacagc agataagaac ttagcatagt atttttcagc aattttagct tcataatccc
     3121 ataaattttt aaaatttctg tttattgtgc tttttgaaag gtcatcaacg gagtatttga
     3181 acattttcat aactttaagt ggcagataaa ctcttttcat tgcaaagtcc tcgccgatgt
     3241 cccgcagtat gttggttatc tgcatagctt ttccaagttc cgcag
//
LOCUS       DAOSHR010000219         3275 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_27414_length_3275_cov_5.234161, whole genome shotgun sequence.
ACCESSION   DAOSHR010000219 DAOSHR010000000
VERSION     DAOSHR010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 3275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3275
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27414_length_3275_cov_5.234161"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..884)
                     /locus_tag="PLU28_09740"
     CDS             complement(<1..884)
                     /locus_tag="PLU28_09740"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOG35379.1"
                     /translation="MIAVTGATGHIGNVLVRELCGKGLEVRAILPPYESAHPISDTQA
                     QIFRADIRDAQSVNKAFEGCETVFHLAGYISIMPFKKKKLFEINVTGTRNVINSCIYN
                     KVDKLIYTSSVHAFSEPQEGRVIDEKTPFDPEKVVGNYAKTKALATLEVLEASTKGLI
                     KAMVLCPSGVIGPYDHRVSEMGELMVRYAAKRMHTYIKGGYDFVDVRDVVQGLINAEK
                     NFKNGEVFILSGYKITVDELLDKLEEITGIKRPKRYIGKTMAYVASLFSCSYYWLTKT
                     KALLTPYSLYTLNSNSNI"
     gene            complement(1064..3061)
                     /locus_tag="PLU28_09745"
     CDS             complement(1064..3061)
                     /locus_tag="PLU28_09745"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOG35380.1"
                     /translation="MKSVRGKMLLSLIASMLVMFVFMAFFLAYQTQNNVVPMTKELVF
                     SVAGARADEVGRWIQGKIDEVKSFAQRDDVQTLDWEKMKDDIIQAAKDRKDTYDIFFV
                     ADLKGSSWNTTGGTANISDRDYFMAIVNQGKDYFVGNPVISRATNAAIVNIAHAIKDE
                     NNRTIGLFGGSVQLTTLSQIISEMKTEDSYGIIVDGTGMVIAHPDTEKVMKFNVADSS
                     KAGYIGLEELGRKMNNNETGFGEITEPDKTKNLILYTNIPNSPNWSLIVSVTTEFLNK
                     AVNQLTLNIVFIIVVIMAMVILISILIGNSVSKPVKRVTNFLLKVSEGDYSLKVDEDM
                     LKKKDEFGTLTKAVQKMIESVNTSMKTIGDHAKDLNSSASSLAAISEETGATSEELAS
                     QSESMAGNAQSSSAAISELSASVEEVASSAQNVSKASQSLSTNSETVTQAAKDGEQSV
                     ENIVSIAQETGKASEHTKTVIRKLSENTENVGKIVESINSITEQTNLLALNAAIEAAR
                     AGEAGKGFAVVADEIRKLAEESKKATQNIANILEETRRHSVQTEEAFTVIDSYIKKIN
                     EEAEKTQGKIKGILHSVEDISAMIQNVSASAQEQSAAAEEMASSMTSAVRMIEDISNQ
                     AKSMSLAVSQQSSSAQQMSMDSQELNTLSESLFEIVKKFKL"
BASE COUNT          795 a          719 c          578 g         1183 t
ORIGIN      
        1 cttatattac tgttgctgtt cagagtatat aacgaatatg gtgtaagaag agctttagtt
       61 ttggtcagcc agtaatatga acaggaaaac aatgaggcta cgtatgccat ggtttttcca
      121 atatatcttt taggtctttt tattccagtt atctcttcaa gcttatccaa aagctcatca
      181 accgttatct tgtacccaga aagaataaaa acctcaccat ttttgaaatt tttttccgca
      241 tttataagtc cttgaaccac atctcttaca tctacaaaat catacccgcc ctttatgtac
      301 gtatgcattc tctttgctgc atatcttacc atcagctccc ccatttcgga cactctgtgg
      361 tcataagggc ctataacccc gctcggacac aaaaccatcg cttttataag accttttgtt
      421 gaagcttcta aaacttccag tgtggcaagg gctttagttt tagcataatt cccgacaact
      481 ttttcgggat caaacggggt tttttcatcg atcacccttc cttcctgcgg ctctgaaaaa
      541 gcatgaactg agctggtata tatcagttta tcgactttgt tatatatgca ggagtttata
      601 acatttctgg ttcctgtaac gtttatctca aagagctttt tctttttaaa aggcattatt
      661 gatatatatc ccgccaggtg aaagactgtt tcgcagcctt caaaagcttt gttaacagat
      721 tgagcatctc ttatatcagc acgaaatatc tgtgcctgag tatcacttat ggggtgggcg
      781 ctttcatacg gcggaagtat ggcacgaacc tctagacctt ttccacataa ttcccgtaca
      841 agaacattgc ctatatgtcc tgttgctccg gttacagcta tcataatttc acccctttcg
      901 ttataaatat taataatcaa atgatttaac tattaatagt ttatactatt ttctatagca
      961 tagtcaaatt ttatagaaac agtataagtc agtaaataca ataaaaaacc cggttatctg
     1021 gaatattcca agataccggg ctttcatgag tttaaagtaa tttttacagt ttaaattttt
     1081 taactatttc aaaaagactt tcagaaagcg tgttgagctc ctgactgtcc atgctcatct
     1141 gctgggcact tgagctctgc tgcgatactg caaggctcat cgatttggcc tgattgctta
     1201 tgtcttctat catccttact gcacttgtca tactgcttgc catctcttct gccgccgcac
     1261 tctgttcctg cgcacttgca gatacgttct gtatcatggc tgagatgtct tcgacactgt
     1321 ggagaattcc ttttatcttt ccctgtgtct tttcggcttc ttcgtttatc ttttttatgt
     1381 atgagtctat taccgtaaat gcttcttctg tctgtactga atgtcttctg gtttcttcga
     1441 gtatgttggc tatgttctgc gtggcttttt tgctttcttc tgcaagtttt cttatttcat
     1501 ctgccactac cgcaaagcct tttcctgctt ctcctgctct ggctgcttct atcgcagcat
     1561 taagcgcaag aaggttggtc tgttcggtta tgctgtttat ggattcaact atcttgccta
     1621 cgttctctgt gttttctgat agttttctta ttactgtttt ggtatgttct gaagcttttc
     1681 ctgtttcctg ggctatgctg actatgtttt caacagactg ttctccgtct ttggctgcct
     1741 gggttactgt ttcggagttg gtcgaaagtg actgcgatgc tttggaaaca ttctgtgcac
     1801 ttgacgctac ctcttcgacg gatgccgaca gctccgatat cgctgctgat gagctctggg
     1861 catttcctgc catgctttct gactgtgatg caagctcttc acttgtggct ccggtttctt
     1921 cagatatcgc tgccagactt gaagctgatg agttcagatc cttggcatga tctcctatgg
     1981 tcttcatgga ggtgttaacc gattctatca tcttctgcac cgctttggta agtgttccga
     2041 actcgtcttt ctttttcagc atatcttcgt cgactttgag cgagtagtct ccttccgata
     2101 ctttgagaag gaagttcgta acccttttta ccggtttgga tacgctgttt ccaattagta
     2161 ttgatatgag gatcaccatc gccataataa ctactatgat gaatactata ttaagtgtca
     2221 gctggttgac tgctttgttt aaaaattcag ttgtgacaga tactataagc gaccagttgg
     2281 gagagttggg gatatttgta taaagtatta gattcttggt tttatccggt tctgttattt
     2341 ctccgaatcc tgtttcatta ttgttcatct ttctaccaag ttcttcaagt cctatatagc
     2401 ctgctttgga tgaatctgca acattaaact tcataacctt ttccgtatcc ggatgggcta
     2461 tgaccattcc tgttccgtcc actattattc cataactgtc ttccgtcttc atctcgctta
     2521 ttatctgaga taatgtggta agctgcaccg agcctccaaa aagccctatg gttctgttgt
     2581 tttcgtcttt tatagcatgg gcgatattga ctattgcagc attggttgcc cttgatatta
     2641 ccggatttcc aacaaaataa tcctttccct gattaactat ggccatgaag taatcacggt
     2701 cacttatgtt tgctgttcct cccgtagtat tccatgaact gcctttaaga tcagctacaa
     2761 aaaatatatc gtatgtgtcc tttctgtcct ttgcggcctg aatgatgtca tccttcatct
     2821 tttcccagtc aagggtctgt acatcatccc tttgtgcaaa gcttttcact tcgtctatct
     2881 ttccctgtat ccatcttccg acttcatcgg cacgtgctcc cgcaacgctg aaaacaagct
     2941 ctttggtcat aggaaccaca ttgttttgtg tctggtaagc caaaaagaac gccatgaata
     3001 caaacatcac gagcatactc gctataagcg agagtaacat ttttcctcta acgcttttca
     3061 tattatgcta ccccctaaca tattaaaaag ttctgagccc ttaccaataa ttataacact
     3121 ttaattttgc tttgtaaaat atttgtttac aacagtaatt aaattatgac tatttaatag
     3181 caagatgtac attcaagaag gttttttaac gatcatttta ttgcatatat gaaatatatt
     3241 aacttttatt attatcactt aataatactt agaac
//
LOCUS       DAOSHR010000220         3255 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_27653_length_3255_cov_6.789375, whole genome shotgun sequence.
ACCESSION   DAOSHR010000220 DAOSHR010000000
VERSION     DAOSHR010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 3255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3255
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27653_length_3255_cov_6.789375"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            416..1144
                     /locus_tag="PLU28_09750"
     CDS             416..1144
                     /locus_tag="PLU28_09750"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOG35381.1"
                     /translation="MNQVIECLKSRASVRAFSEEKIPRHIKEVVLNMAMAAPTAGNQQ
                     LYTIIEVNDQEKKDILAESCDHQPFIAQAPWVLLFCADQLKWYEAYKLANLCPREPGP
                     GDFLLAVEDTMIAAQNAVIAAQSLGIGSCYIGDILEHKEVHQKMFNLPQIVVPVSLIV
                     FGYPKSKAEAPKNRFPLNCVVYEDSYRSLSRKEWENMTEFRRRGRDFDSWMKAFFERK
                     YQSDFSEEMNRSVSEYLKGFMDKR"
     gene            complement(1163..1549)
                     /locus_tag="PLU28_09755"
     CDS             complement(1163..1549)
                     /locus_tag="PLU28_09755"
                     /inference="COORDINATES: protein motif:HMM:NF022158.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HOG35382.1"
                     /translation="MFGGSDPFGSFGMFNFIFYGVFFLVIGMFAVILISIVLGWFKNQ
                     RSPLIEVQAKVVAKRDHTSIHSRNRSTSSINTYSSFTTYYVTFQFDTGDRIELKVPYD
                     EFGLLIEGDNGKLKFQGTRFIQFERL"
     regulatory      1767..1898
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            2030..2677
                     /locus_tag="PLU28_09760"
     CDS             2030..2677
                     /locus_tag="PLU28_09760"
                     /inference="COORDINATES: protein motif:HMM:NF024231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOG35383.1"
                     /translation="MNEKARWMTAAAMLCALSYASVFIARVPVVLFLKYEPKDVIITI
                     GGFIFGPMTAVFVSAVVSLLEMITISSTGVIGFIMNVISTCAFACTAAYIYKKHQTAK
                     GALAGLITASLAMTAVMLLWNYLITPIYMNYPRQAVVKLLLPAFLPFNLLKSGINTAL
                     VLILYKPVVAALRQTKMISEGQVKTEKKINAGVAFFSVFVLITCVLVVMVMNKTI"
     gene            2773..3216
                     /locus_tag="PLU28_09765"
     CDS             2773..3216
                     /locus_tag="PLU28_09765"
                     /inference="COORDINATES: protein motif:HMM:NF016356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HOG35384.1"
                     /translation="MNILVSACLLGLNCRYDGKGQPEKKVLDLIEKYNLIPVCPEVYG
                     GLKTPRKPAEIKEGKVVTVLGEDVTENFLRGARETLKIAELFKCPYAILKERSPSCGF
                     GRVYDGTFSGKIIFGNGITADLLDKKGIKVFNENTFEKFLTEIEK"
BASE COUNT         1049 a          540 c          672 g          994 t
ORIGIN      
        1 attcactgtt ttcacgttcg agttcaacaa tatgtaatcg tatctcatca tctgtaagta
       61 taagctttaa taattctttc tccagcatga tattgtctaa tacttaatac ctagctgttc
      121 agatgctttt tttactccta tttcatctga tagtttcaat acttcttctt tgaattcttt
      181 actgtacttt ctcattttta aacacctctt ttgaggtgtt tgtcgactct acttttatgg
      241 tttctatcca gtcaatatgt tttataatat ataatatata aaagcataaa acatacaaat
      301 atcattttat tttaaaatcg ggaaattttt tttaagaaaa ccattgacag ttctatcaag
      361 caggaaacgg atgataagcc ataacaagct taaatattga aagggggaag gttttatgaa
      421 tcaggttata gaatgtctaa aaagcagagc ttccgtgaga gctttttcag aagaaaagat
      481 acccaggcac ataaaagaag ttgttcttaa catggcaatg gctgcaccta cagcaggaaa
      541 tcagcagctt tataccataa ttgaagtaaa cgaccaggag aaaaaggata ttttggctga
      601 aagttgtgat catcagcctt ttattgcaca agctccgtgg gttttgctct tttgtgctga
      661 tcagcttaaa tggtatgaag cgtacaagct tgcgaacctc tgtccacgag aaccgggacc
      721 gggagatttt ttgcttgctg ttgaggatac tatgatagca gcgcaaaatg cggtaatagc
      781 agcccaaagt cttggaatag gttcatgcta tataggagat atccttgaac acaaagaagt
      841 acatcaaaaa atgttcaatc ttccacagat agttgttcct gtgagtctga tcgtgtttgg
      901 gtatccaaaa agtaaggctg aagctccaaa aaacagattt cccctgaact gtgttgtata
      961 tgaagacagc tacaggagtc taagcaggaa agaatgggag aatatgaccg agttcaggcg
     1021 cagggggcgc gattttgaca gctggatgaa agcttttttt gaacggaagt accaatcaga
     1081 tttctctgag gagatgaaca gatccgtcag tgaatatctg aaaggtttta tggataagcg
     1141 gtaaaaacag agttttagta gattataatc tttcaaactg tatgaatctg gtgccttgaa
     1201 acttcagttt tccattgtcg ccttcaataa gaagtccgaa ctcatcatac ggtactttaa
     1261 gttcgatcct gtcaccggtg tcaaactgaa aagtaacata atatgtcgta aatgaactgt
     1321 aagtattaat agaagaagtt gatctgttcc gtgaatgaat ggatgtatgg tctcttttgg
     1381 caacaacttt ggcctgtact tctataagag gacttctctg atttttaaac cagccaagaa
     1441 caatacttat aaggataacg gcgaacatgc ctattaccag gaaaaacact ccgtagaaga
     1501 tgaagttaaa cataccaaaa gaaccgaaag gatctgatcc accaaacata ttttcacctc
     1561 acagaaagtt ataagaatat tatatcacac ttataaaagg agtatttcaa aggaagcttg
     1621 aggtatctgt ggatagatat gatctattaa atgctgttat agctgaaata aatgcgttat
     1681 cgtctttaaa tactttgttt tttattgcac aaagtattga caaaaatatt ctgaagcggt
     1741 ataataaaaa taacatttga atagagattt gtcttcaggg cagggtgaga ttcccgatcg
     1801 gcggtaaagt ccgcgagcgc tttaagcgca ggagcgtaag ctcaggattt ggtgaaattc
     1861 caaaaccgac agtaaagtct ggatgaaaga agatgtgtta aaagcttaag cttgtgcagt
     1921 taccgttaaa ggtaattgta tcggcggcag ataagacggg ctcttataat tttaacacct
     1981 gaaagacaac caatctttca ggtgttaatt tattttagga ggctttgata tgaatgaaaa
     2041 agcaaggtgg atgactgcgg cggctatgct gtgcgcactc tcctatgcat cggtgtttat
     2101 tgcaagggtg cctgtggttc tatttttaaa gtatgaaccc aaagatgtta ttattaccat
     2161 tggtgggttt atcttcggac ctatgactgc cgtttttgtt tctgccgtgg tttctctttt
     2221 ggagatgatt actattagca gcacaggggt gatagggttt ataatgaacg ttatatctac
     2281 gtgtgcgttt gcatgcacag cagcctatat atataaaaaa catcagactg ccaagggggc
     2341 actagccggt cttataaccg ccagtcttgc aatgacagct gttatgcttt tgtggaatta
     2401 ccttataacc cctatatata tgaactatcc tcggcaagca gttgtcaagc ttttgctgcc
     2461 agctttcctg cccttcaatc ttttaaaaag cggtatcaat acagctcttg ttcttattct
     2521 ttataaacct gtggtagcgg ctctaagaca gactaaaatg atctctgaag gacaggtaaa
     2581 aactgaaaaa aagataaacg caggagttgc ttttttttca gtctttgtac ttattacatg
     2641 tgtacttgta gttatggtta tgaacaagac gatataaaaa acttcctcat aaagaggaag
     2701 ttttttgttg aatacagaga aaaaagttat aatatattat atatgttgaa aaacatagat
     2761 aaaggagaaa gcatgaatat actcgtaagc gcatgcctgc ttgggcttaa ctgccgttat
     2821 gatggaaaag gccagcctga gaaaaaagtt ctagacttga ttgaaaaata caatcttatt
     2881 ccggtctgtc ccgaggtgta cggaggctta aaaactccta gaaaaccagc cgaaataaaa
     2941 gaaggaaaag tcgttacggt cttgggagaa gacgttacgg aaaacttctt aagaggtgca
     3001 cgggaaaccc ttaaaattgc agagctcttt aaatgtccgt atgccatact caaagaaaga
     3061 agtccttcat gcggatttgg aagagtttat gatggcactt tcagcggaaa aattattttt
     3121 gggaacggta taactgctga tcttttggac aaaaaaggga taaaagtttt caatgaaaat
     3181 acttttgaaa aatttttaac agagatagaa aagtaagctt atatttgagg ctttataaac
     3241 ggttattata gtata
//
LOCUS       DAOSHR010000221         3218 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_28125_length_3218_cov_3.508062, whole genome shotgun sequence.
ACCESSION   DAOSHR010000221 DAOSHR010000000
VERSION     DAOSHR010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28125_length_3218_cov_3.508062"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2594
                     /locus_tag="PLU28_09770"
     CDS             <1..2594
                     /locus_tag="PLU28_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35385.1"
                     /translation="SPLDLESGVDPKTIFRWSVDQEDNYRYKVYVSENPFVLEKDLKV
                     SNLIAKFFTGEILRPDTTYYWKVVALKENEVYESPIFRFSTRSLNNGDISWLHFAEYD
                     QMLFFNNMMVGRKGDRVDILSENKNILYSKIFNEKITGLYALDRLYIVLENMVVRIEP
                     DLKENTEEIEEKVKAIAGRVYYSDKYLYILSTSGKFNRLCFEPIKKVLFLNDFFYILS
                     DKKVYMYEPMKGQVTQKDFEDDLCDMVLSTYQDRIIVMGKNGLYTLDMKLGLKNKYIF
                     QIDMERKIYPFFDKIAMISGKNRLTFFDSDLKWTQENMFDEDIYYFIPINPTAYMLAG
                     NYIKTFDTQGGLLWAYSSLNPVQLISEPQYGAKGILAGIKDFVSRYIIFYEKMDAAII
                     NNPVYKPLPKVIIPKEYEPEEVQSVKTDPQPFTQPEKPVSKTEQPVEVTPQPTNTQQP
                     TNQDIQPVQPQPKTEDTPASTHTKAQDKPTEPATSSTAPIVPINEIPQTEPTKAATPQ
                     EIKKDYTEIPNALSNYGYTIKTYENIMYIAGYEISDNNWQAKVTQIASTLEVEKEFLF
                     GGKGTEFFKDVLITKDASEVISITAIGDTTSVGYSGDAYLVSFLPDGTVLYESNYGDL
                     GRDNGVKIKEVDKNGYAIIGNYYVNNKLSDIFVSRYSKDGTRIWLSTFGGKNIEMAVD
                     CEVTPDQGLLITGATKSFGYGKYDVYAIKVDFYGREKWTNTFGDDKDNLPVGIITFSN
                     KKYILYETKEELRKNSHNIVEVDHEKGFKLSFKYIPDYEENLLGFSNLGGTVYAYGYR
                     MVKGKKAGLIYTVDMKNSLFTDPKVFEMDGDFEITSMTVLTNDVYALGNLNNKKLSMI
                     LMNINKK"
     gene            2627..>3218
                     /locus_tag="PLU28_09775"
     CDS             2627..>3218
                     /locus_tag="PLU28_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HOG35386.1"
                     /translation="MDVCSAFNKCGGCQNLGIDYQIQLETKETKVLQLFEQSGIIIEK
                     YEGITPSPNIYHYRNKMEYSFGNEYKDGPLNIGLKGRKRLIDVYYAPDCMIAPHDFNT
                     IVSFSREFFLSENLTFRNFLRHQGYLRHLSVRQGTHTHEILVHLSTDKDYEKDHIVDR
                     WAQMLTGLKLEGTIKGILHSRSDTRSNMFAVENTRTL"
BASE COUNT         1160 a          645 c          627 g          786 t
ORIGIN      
        1 tttcaccttt agaccttgaa agcggggttg atcccaagac aattttcagg tggagtgtcg
       61 atcaggaaga caactacaga tataaagtgt atgtatcaga aaatccattt gtactggaaa
      121 aggatttgaa agtatcaaat ctgattgcca agttttttac aggggagata ctcagaccgg
      181 acaccacgta ttactggaag gttgtcgcat tgaaggagaa cgaagtatat gaaagtccca
      241 tattcagatt ttcgaccaga tcgctgaaca atggggatat aagctggctg cactttgctg
      301 agtacgatca gatgctgttt tttaacaata tgatggtcgg acgcaaagga gatagagtag
      361 acatactttc cgagaataaa aatattttgt actcaaaaat atttaatgaa aaaataaccg
      421 ggctttatgc tctggacagg ctttatatag tacttgagaa tatggttgtg agaatagagc
      481 cggatctcaa agaaaatacc gaagaaatag aagaaaaggt aaaagccatt gccggaagag
      541 tttactactc agacaaatac ctatacatac tttccacatc cggaaagttc aacaggcttt
      601 gctttgaacc gatcaaaaag gttttgttcc ttaatgactt tttctatatt ctttcagata
      661 aaaaagtcta tatgtatgaa cctatgaaag gacaagttac ccagaaagat tttgaagatg
      721 atctttgtga tatggtgctt tcaacttatc aggacagaat catcgtaatg ggaaaaaacg
      781 gtctctacac ccttgacatg aagcttggcc ttaagaacaa gtatatcttc cagatcgaca
      841 tggaaagaaa gatataccca ttttttgata aaattgccat gatcagcggt aaaaacagac
      901 ttactttttt tgattccgac ctgaagtgga cacaggaaaa tatgtttgac gaagacatat
      961 actactttat tcctatcaat ccgacagctt atatgctggc tggtaattac atcaagacct
     1021 ttgataccca aggtggactt ttgtgggcat actcatcgct taatccggtt cagctcatct
     1081 ctgaacccca gtatggagca aaaggtatct tagcaggaat aaaagacttt gtatcaagat
     1141 acataatatt ctatgaaaag atggatgcag ctattataaa caatccggtg tataaaccgc
     1201 ttcccaaagt tattattcca aaagagtacg agcctgaaga agtacagtcg gttaaaacag
     1261 atccgcagcc ttttacacaa ccggaaaaac ctgtttcaaa aactgaacag cctgtcgagg
     1321 taacaccaca gcctaccaat acacagcaac caactaacca agatattcag cctgtacagc
     1381 cacagccaaa aacagaagac acaccggcat ctactcacac caaagcacag gacaaaccga
     1441 cagaaccggc aacaagcagc accgctccga tcgtaccgat aaatgaaata ccacaaaccg
     1501 agccgactaa agcggctact ccgcaagaga taaaaaaaga ttatacagaa attcccaatg
     1561 cgctttcaaa ctatggatat actatcaaaa catacgaaaa tataatgtat atagccggct
     1621 atgaaatcag cgacaacaac tggcaggcta aggttacaca gattgccagc acccttgaag
     1681 ttgaaaaaga gttcctcttc gggggcaaag gcacagagtt tttcaaagac gttcttataa
     1741 ccaaggatgc ctccgaagtt atcagtataa cagcaatcgg tgacactact tccgtaggat
     1801 acagtggaga tgcctatctt gtatcgttcc ttcctgacgg aactgttcta tatgaatcta
     1861 actacggtga tctgggaaga gacaacggag taaaaataaa agaagttgac aaaaacggtt
     1921 atgccataat aggaaattac tatgtgaaca acaaactttc agatatattc gtttccaggt
     1981 attcaaagga cggaacaaga atatggctaa gcacctttgg cggtaaaaac atagagatgg
     2041 ctgttgactg tgaggtaacc ccagaccagg gactgctcat aaccggtgcc accaaatcat
     2101 tcggatacgg taaatacgac gtttatgcca taaaagtaga tttctacgga agagaaaaat
     2161 ggaccaacac ctttggtgac gataaagata acctacccgt aggaataatt acattctcaa
     2221 acaaaaagta tatactctat gaaaccaagg aggagcttag gaaaaactcc cacaacattg
     2281 tggaggtaga tcatgaaaaa ggattcaaac tttcattcaa atacattcct gattatgaag
     2341 aaaaccttct gggtttttcc aatcttggag gaacagttta cgcttacggc taccgcatgg
     2401 taaaaggcaa aaaagctggc cttatatata ctgttgatat gaaaaacagc cttttcaccg
     2461 atccgaaagt ttttgaaatg gacggagatt tcgaaattac aagcatgaca gttctgacaa
     2521 acgatgtcta tgctcttgga aaccttaaca ataagaaact aagcatgata cttatgaaca
     2581 taaataaaaa atagaactgc aaaaaaacaa ataaaaggag aacaacatgg acgtatgctc
     2641 ggcctttaac aaatgcggag gatgccaaaa tcttggcata gactaccaga ttcagcttga
     2701 aaccaaagaa acaaaagtgc tgcagctctt tgaacaaagc ggtataatca tagaaaaata
     2761 cgagggaata accccatcgc cgaatatata ccactacaga aacaagatgg agtactcttt
     2821 cggaaacgaa tataaagacg gacctctcaa tatagggctt aaaggaagaa aaagactcat
     2881 agatgtatac tatgcacctg actgcatgat agctccgcat gatttcaaca cgatcgtaag
     2941 cttttcaagg gagttctttt tgtccgaaaa tcttaccttc agaaactttc tcagacatca
     3001 gggctacctc agacaccttt cggtaagaca aggaacccat acccacgaaa tccttgtaca
     3061 cctttccaca gacaaagatt atgaaaaaga ccatatcgtt gacagatggg cccagatgct
     3121 cacaggactt aaacttgaag gaaccataaa aggaatactt cacagcagat cagataccag
     3181 atcgaatatg tttgccgtgg aaaacacaag aaccctgt
//
LOCUS       DAOSHR010000222         3210 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_28232_length_3210_cov_5.051664, whole genome shotgun sequence.
ACCESSION   DAOSHR010000222 DAOSHR010000000
VERSION     DAOSHR010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3210
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28232_length_3210_cov_5.051664"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..856
                     /locus_tag="PLU28_09780"
     CDS             <1..856
                     /locus_tag="PLU28_09780"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOG35387.1"
                     /translation="EGSFIKSGMFISTSVSQADICISRHNSQPGKYSLVMTAQTIEFY
                     DTADNLFYTLNVTDEMTDIEIAIIKTISIVNGRKPFDLYASKARYVNKLSKFLQGLVV
                     TLEVEDERTNMSHSQRVALYSVEFSRYLGLDEDEQHKIKELCMLHDIGRIGLEQLMLF
                     SPTRISEFESWDLEHTITGSIFLSSLDELWFAVPVVRSHHENWNGSGYPDGLKENEIP
                     FYARLVGIVDWFDIATHTASSEFDGIMSVEDAIVSIDKNSGKLFDPVLGPKFVLFMKQ
                     YLQTNTFL"
     gene            941..2245
                     /gene="asnS"
                     /locus_tag="PLU28_09785"
     CDS             941..2245
                     /gene="asnS"
                     /locus_tag="PLU28_09785"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HOG35388.1"
                     /translation="MKADWIYIHDMKNYIDKKVEFRGWLANRRSSGKIQFLQLRDGSG
                     FIQAVVELSRVGEDVFNSSSALKMESSLTLIGTVVKNEKAPNGIELLAEDVKVVGIPN
                     EDYPISKKDHGIDFLMDNRHLWLRSKRQFHILNIRHNIIKATRDFYNNRDFTLVDTPI
                     FTGSIGETAGNLFEIDYFDYGKVYLTQTGQLYLEAAAMSLGKVYNLGPTFRAEKSKTR
                     RHLIEFWMNEAEVAYYRHEDNMKLQEDLVSYIVKHVLENCAESLTALERNTSVLEKVQ
                     APFPRITYTDAVKLLQKKGFDIKWGDDIGADEETGVAKEFDKPVIVEKYPRELKAFYM
                     QPDPENPQVVMCDDMLAPEGYGEIIGASERIWEKDVLIDSLKSKKLPVEGYQWYLDLR
                     EYGSVPHSGFGMGIERVVAWLCGLEHIREAIPFARTLYRVYP"
     gene            2336..>3210
                     /gene="ruvB"
                     /locus_tag="PLU28_09790"
     CDS             2336..>3210
                     /gene="ruvB"
                     /locus_tag="PLU28_09790"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOG35389.1"
                     /translation="MIFGNNNESTQRILNSTELGDEVSIVSLRPKLLNEYIGQSKIKE
                     RLNVSIQAAKKRKDVLDHVLLAGPPGLGKTTMANVIANEMKSNIQVTSGPVLEKAGDL
                     AAILTNLAGGDILFIDEIHRLNRSVEEILYSAVEDFKLDIVIGKGPSARSIRLDLKPF
                     TLIGATTRTGLIAAPLRSRFGIILEMSFYTDDDLKLIVCRSAELLKAAISEEAALLIA
                     RRSRGTPRIANRLLKRVRDTAQVKGKSRIEVSDVDETMSMLDIDTYGLDEMDRKLLNT
                     VTQNYKGGPVGLKALA"
BASE COUNT         1015 a          547 c          711 g          937 t
ORIGIN      
        1 tgaaggttct tttataaagt caggtatgtt tataagcact tcggtttctc aagccgacat
       61 atgtataagc agacataatt ctcaacccgg aaagtactct cttgttatga cggctcaaac
      121 aattgagttt tatgacactg cagataatct tttttatact cttaatgtga cagatgaaat
      181 gactgatatc gaaattgcaa taattaaaac catatcgata gtaaacggca gaaaaccttt
      241 tgatctttat gcctcgaagg cccgttacgt aaacaaactt tccaaattcc tccaaggact
      301 ggttgtaacg cttgaagtag aagatgaaag aaccaatatg agccattctc aaagagttgc
      361 tctgtatagt gtcgagttct ctaggtatct tggtcttgat gaggatgaac agcacaaaat
      421 aaaagaatta tgtatgcttc atgacatagg aagaattggg ctcgaacagc ttatgctttt
      481 ttctcctacc agaataagcg agtttgaatc atgggatctg gaacatacga ttacaggatc
      541 aattttttta agttctcttg atgagctatg gtttgctgta cctgtagtcc gtagtcatca
      601 cgaaaactgg aacggctcag gctatcccga cggtcttaag gaaaatgaaa taccttttta
      661 tgcacggctg gtaggaatag ttgactggtt tgatattgcc actcatacag cttcttctga
      721 atttgacggt ataatgagtg tggaagatgc tatcgtttcg attgacaaaa attcaggaaa
      781 gctttttgac cctgttttag gcccaaagtt tgttttgttt atgaaacagt accttcaaac
      841 taatactttt ttatagcccg tatgagagta cgggttttta tttactacaa tgtttatggt
      901 ataattgaat ttataataac ttatgatcag gaggggtttt atgaaggctg attggatata
      961 tatccatgac atgaaaaact atatagataa aaaagttgaa ttcagaggct ggctggctaa
     1021 caggcgatcc agcggaaaga ttcagtttct gcagctcaga gatggctcag gattcataca
     1081 agccgtggta gaactttcca gagtcggaga agatgtattt aactcttcat ccgctttgaa
     1141 gatggaatca agtcttactt taatcggaac tgtcgtaaag aatgagaaag ctccgaatgg
     1201 aatagaactt cttgcagaag atgtaaaagt agtaggtatt ccaaatgagg attaccctat
     1261 atcgaaaaaa gatcacggaa ttgattttct tatggataac aggcatctct ggctgagatc
     1321 caagagacag tttcatattc tcaatataag gcacaacata ataaaagcaa cacgtgattt
     1381 ttacaacaac cgagatttca ctcttgtaga tactccgata tttaccggtt caataggaga
     1441 aactgccgga aatctttttg aaatagatta ctttgattat ggaaaggtat atcttaccca
     1501 gacaggacag ttatatcttg aagctgccgc catgtctctg ggaaaggttt ataaccttgg
     1561 tcctaccttc agggctgaaa aatccaaaac cagaagacat cttattgagt tctggatgaa
     1621 tgaggcagaa gttgcttatt acaggcatga agacaatatg aagctccaag aggatcttgt
     1681 ttcatatatt gttaagcatg tgcttgaaaa ctgtgcagag agtcttacag cacttgaaag
     1741 aaatacttca gttttggaaa aagtacaggc tccttttcca aggataacct ataccgacgc
     1801 tgtgaagctt cttcagaaaa aaggtttcga tataaaatgg ggagacgata taggcgccga
     1861 tgaagaaaca ggtgtggcta aagagtttga taaaccagta attgtggaaa aatatcctag
     1921 ggaactcaag gcgttttata tgcagcctga tcccgagaat ccgcaggttg ttatgtgtga
     1981 cgatatgctt gctcccgaag gttacggaga aataatagga gcatcagaaa gaatatggga
     2041 aaaggatgtt ctgatcgata gtcttaaatc aaaaaaactt ccggttgaag gatatcaatg
     2101 gtatcttgat ctgagggaat acgggagtgt tcctcacagt ggattcggaa tgggaataga
     2161 aagagtcgtt gcatggcttt gtggcttgga acatataaga gaggctattc cgtttgcaag
     2221 gactctttat agagtctatc cgtaatttat tacagaattt tcaggatatt gaactgtttt
     2281 tttgattctt ctgttccagt tgttttttat aatatccgac ggatcggggg aaaagatgat
     2341 atttggaaat aataatgaat caacgcagcg aattttaaat tctaccgagc ttggtgacga
     2401 ggttagcata gttagccttc gtcccaagct cttaaatgaa tatataggtc aatcaaagat
     2461 aaaagaacgt cttaatgtca gcatacaggc tgctaaaaaa cgcaaagatg tcttggatca
     2521 tgttctgctt gcaggacctc ccggtctggg aaaaaccact atggcgaatg taattgccaa
     2581 tgagatgaaa tctaacatac aagtaaccag cggacctgtt cttgaaaaag ccggggatct
     2641 ggcagctatt ttgaccaatc ttgccggcgg ggatattctg tttattgatg agattcacag
     2701 actaaacaga tctgtggaag agattttgta ctctgcagtt gaagacttca agcttgatat
     2761 agtaattgga aaaggaccgt ctgccagatc aataagactt gatctcaagc cttttactct
     2821 tattggggca accacaagaa caggattgat agctgctcct ctcagaagca ggtttggcat
     2881 cattctcgaa atgagttttt atacggacga tgatttaaaa cttattgtat gcagaagtgc
     2941 cgagcttcta aaagccgcca tatccgagga ggctgctttg cttattgccc gcagatcgcg
     3001 tggtactcct agaatagcca acagactgct aaaaagagtc cgagatactg cgcaggtgaa
     3061 aggaaaaagc agaattgaag tttctgacgt tgatgagaca atgagtatgc ttgatataga
     3121 tacatacggt cttgatgaaa tggatagaaa gcttcttaat actgtaacac agaattataa
     3181 aggtggtccg gtgggactta aagcattggc
//
LOCUS       DAOSHR010000223         3202 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_28334_length_3202_cov_6.177312, whole genome shotgun sequence.
ACCESSION   DAOSHR010000223 DAOSHR010000000
VERSION     DAOSHR010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 3202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3202
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28334_length_3202_cov_6.177312"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="PLU28_09795"
     CDS             <1..489
                     /locus_tag="PLU28_09795"
                     /inference="COORDINATES: protein motif:HMM:NF039826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5685 family protein"
                     /protein_id="HOG35390.1"
                     /translation="DAFISRYEELIYSQTEAEKGKSDFDDFARPSSEIMYFIAQKEFS
                     GMEFDVELFSKACGTLVYLIDAVYDFKTDIKKSAFNGIAKSFGITDITRIAAKDFERL
                     VFTYDICVKSIYESLNTTEFKNNQLVHKLLSYSFTYHRNRIIEIFKGEKNENNRRACK
                     KR"
     gene            455..676
                     /locus_tag="PLU28_09800"
     CDS             455..676
                     /locus_tag="PLU28_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35391.1"
                     /translation="MKTTEELVKKGDFKRLFSEYKEKTELKQKMDEFRQGYYNYRRND
                     CMDCLACFGGLACADTLCECSGGDLCRCI"
     gene            722..1210
                     /locus_tag="PLU28_09805"
     CDS             722..1210
                     /locus_tag="PLU28_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35392.1"
                     /translation="MKNPAHELALTGIMLAGSYLVYYFSQIFRVAPNIIPLTIPLALF
                     LLKRRHALLFSLAFFLLLTVSGFLSKAAGFFFLLLIPVAFGRFSENKKKMVLLIIFAG
                     SLGFWTVYSFFSFLLPAFFTAKLSLVFLLFLLYTFAIQIYNLILSLVYARIKNYIGRD
                     KK"
     gene            1207..3168
                     /locus_tag="PLU28_09810"
     CDS             1207..3168
                     /locus_tag="PLU28_09810"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOG35393.1"
                     /translation="MTKKIFSAESLFDFSFISELEFSPDGKEYLYVRNSLNRKDNAYV
                     KEIFLGRKNRTPLKIAGGNSFSPSHSPDGKYIVFSSLREKEFRGSELYLIERDGGEAM
                     LLCKLEKEFIRIKQWDKHSKGLFLLTSKQIVEKDKKDDAVEINEVPFWTNGGTFTNDT
                     EFFFYYISLNGKKSEIPLKKILSEGLNKQSNKKIRINEFSLSPDNNMVAFTVCEEPEK
                     FPYKSSIFIYNTKEKSATKLSIKGTSAFNLCWKDDSSKIAYLGENEELGLDGNSKLYI
                     YDFAKMKEECISKKFDKTLTICSAVNSDVFSSKNMKMRWKNDKIYFTILDKDSNAIYS
                     IDEKKNLKIESIEHGSYSAYDVNNNGEIIYSYDNFTVPQEVYILKNQKITRLTSINKK
                     ITNEYRILSYQKNTVKTDSSEIDSWIVMPSSHEEKIPAILEIHGGPKTAYGNSFMLEF
                     QILAAQGYGVIFSNPRGSDGYGEEFCDIRKKYGTIDYEDLMAVMDFYEKNYDIDSKRT
                     GVTGGSYGGFMTNYIVTKTDRFKAAVTQRSISNWFSMYGTSDIGYFFTRDQIGFEPWE
                     NKEEYIEKSPLFQVKNVKTPLLLIHSFEDYRCWVVEAMQIFTSLRVLGVKSKLVIFKG
                     ENHELSRSGKPFNRVKRYNEILKWFRENL"
BASE COUNT         1134 a          481 c          618 g          969 t
ORIGIN      
        1 gatgctttta tcagcagata tgaagagctt atttattcac aaactgaggc ggaaaaagga
       61 aaaagtgatt ttgatgactt tgcaagacca tcttccgaaa taatgtattt tattgctcaa
      121 aaagagttca gtggcatgga gtttgatgtg gaactctttt caaaggcctg tggaactctt
      181 gtatatctta tagatgcagt ctatgacttt aaaactgata tcaagaaatc agcatttaac
      241 ggtatagcaa aaagctttgg aattacagat attacaagaa tagctgcaaa agactttgaa
      301 aggcttgttt tcacctacga tatctgtgta aagagcatat atgagtcttt aaatactaca
      361 gagtttaaaa ataaccagtt ggtgcataag cttttaagct attcctttac atatcacaga
      421 aatagaataa tagaaatttt taagggggaa aaaaatgaaa acaacagaag agcttgtaaa
      481 aaaaggtgat tttaagaggc ttttcagtga gtacaaggaa aaaacagagt taaaacaaaa
      541 gatggatgag ttcaggcaag gttattacaa ttacagaaga aatgactgta tggattgtct
      601 tgcgtgtttc ggaggacttg cctgcgccga tacgttgtgc gaatgttcgg gcggagatct
      661 ctgcagatgt atataaagag cctaagggct cttaattttt atttgacgga agtgtgatat
      721 tttgaaaaat cccgcccatg agcttgcttt gacaggaatt atgcttgcag gttcatattt
      781 agtatattat ttttcacaga tatttagagt agcccccaat ataatacctc ttaccatacc
      841 tcttgccctt ttccttctga aaagaaggca tgctttgctt ttttctcttg ctttttttct
      901 gctccttaca gtaagcggat ttttaagcaa agcagccggt tttttctttc ttttacttat
      961 accagttgct ttcggtagat tttctgaaaa caagaaaaaa atggttttac taattatttt
     1021 tgcaggcagt ctgggcttct ggactgtata tagtttcttt tcatttcttc ttccagcgtt
     1081 ttttacagcc aaactttcct tggtttttct gctctttttg ttatacacct tcgcaataca
     1141 gatatataat ctgatacttt cactagttta cgcacggatt aaaaattata tagggaggga
     1201 caaaaaatga ccaaaaaaat tttttcagca gaatctttgt ttgacttcag ctttatttcc
     1261 gaactggagt tttcccctga cggaaaagag tatctgtatg tacggaactc attaaataga
     1321 aaagataacg catatgtaaa agaaatattt ctgggcagga aaaacagaac tcccttaaaa
     1381 attgccggtg gcaatagttt ttctccttcg catagtccgg atggcaagta tatagtcttc
     1441 agctctttaa gagaaaaaga gttcagaggt tctgaacttt atctgataga acgtgacgga
     1501 ggagaagcaa tgcttttgtg taagcttgaa aaagagttca taagaatcaa gcaatgggat
     1561 aagcactcta aggggctttt tcttctcaca agcaaacaaa tagtcgagaa ggataaaaaa
     1621 gatgatgcag ttgaaataaa tgaagttcct ttttggacga acggcggtac ttttaccaac
     1681 gatacggaat ttttctttta ttatataagt ctgaacggta aaaaaagtga gataccgcta
     1741 aaaaaaatat tatctgaagg tttgaataag caaagcaata aaaaaataag gataaatgaa
     1801 ttttcacttt cgccggataa caatatggta gcttttacgg tatgtgagga gccggaaaaa
     1861 tttccttaca aatcatctat ttttatatac aatacaaaag aaaagagtgc aacgaagttg
     1921 agtattaaag gaacctcagc atttaacctg tgctggaaag atgactcttc aaagatagcg
     1981 tatcttggag aaaacgaaga acttggactt gacggaaact ccaaacttta tatatatgat
     2041 tttgctaaaa tgaaggaaga atgtataagc aaaaagttcg acaaaacttt aacaatatgt
     2101 tctgctgtta attcagacgt attttcaagc aagaatatga aaatgcgttg gaaaaatgac
     2161 aaaatatact ttacaatact tgataaagat tcaaatgcaa tatatagcat cgatgaaaaa
     2221 aagaacctta aaattgaaag tattgagcat ggttcttatt ctgcgtatga tgtaaataat
     2281 aacggggaaa taatatattc atacgataat tttactgtac ctcaggaagt ttatattctg
     2341 aaaaatcaaa aaataacaag gcttacatct ataaataaga aaataaccaa tgagtatcgt
     2401 atactctcat atcaaaaaaa tactgtaaaa acagattctt ctgaaataga ttcatggata
     2461 gtaatgccaa gctcacatga agaaaagatt ccggccatac tcgaaataca cggaggtccc
     2521 aagactgcat atggaaattc ttttatgctt gaatttcaaa tacttgcggc acaggggtat
     2581 ggtgttatat tttctaatcc aagaggaagc gatggatacg gcgaagagtt ctgcgatata
     2641 agaaaaaaat atggaacaat tgattatgaa gatctgatgg cagttatgga tttttatgaa
     2701 aaaaactatg atatagacag taaaagaaca ggtgtgacag gcggctccta tggaggtttt
     2761 atgaccaact acatagtgac aaaaactgac agattcaaag ctgctgtaac ccaacgttct
     2821 atatcaaatt ggttcagtat gtacggaaca tctgatatag gatacttttt tacacgagat
     2881 cagataggat tcgaaccgtg ggagaacaaa gaagagtata tagaaaaatc cccgcttttt
     2941 caggtaaaaa atgtaaaaac tccactgctt cttatacatt catttgaaga ttacagatgt
     3001 tgggtagtag aagcaatgca gatctttacg tcattaaggg ttttgggggt aaaatccaag
     3061 ctcgtgattt tcaaaggaga aaaccacgag ctttcaagat cgggaaaacc tttcaacaga
     3121 gtaaagagat acaatgaaat attaaagtgg ttcagagaaa atctatagag taactgagat
     3181 ttgttacttt agaagatatt tt
//
LOCUS       DAOSHR010000224         3154 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_28926_length_3154_cov_5.072281, whole genome shotgun sequence.
ACCESSION   DAOSHR010000224 DAOSHR010000000
VERSION     DAOSHR010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28926_length_3154_cov_5.072281"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            352..612
                     /locus_tag="PLU28_09815"
     CDS             352..612
                     /locus_tag="PLU28_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35394.1"
                     /translation="MYRYIGYTGGMTGIGMFFVVVAVVLLVVILSVVLYKVLANKSSK
                     NEKHEVPTKEEDIAIELLRQEYAKGSINEEEYVKRKTFLINN"
     regulatory      708..886
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            1059..2585
                     /locus_tag="PLU28_09820"
     CDS             1059..2585
                     /locus_tag="PLU28_09820"
                     /inference="COORDINATES: protein motif:HMM:TIGR03458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008775 - acetate CoA-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate CoA transferase"
                     /protein_id="HOG35395.1"
                     /translation="MIKQLIKNSFVMSKVMSAYEAALMIKDGMTVATSGFTPSGYPKE
                     VPKALADIVESTGKKIKLTLLTGASVGEEIDGRWTQAGIICKRMPYQTNNLLRNSINR
                     GECEFSDIHLSNFAQNIRYGFYGEIDIAIIEAVGITEEGFIIPSTSVGASPVYVQKAR
                     KVIVEINTSLSKQLEGFADVYMPDDPPHRLPIPLFSAQQRIGTKYIPCNKEKIAAVVI
                     SEIQDKHTVFSECDDTALKISNHLLNFLHNEIIMNRVPKTLLPLQSGVGNIANAVLSG
                     IEKSNFSNISFYTEVIQDSMLKLIKKGKAVFASGTALTLSTDCFKEFENNISFFKERV
                     VLRPQEISNSPEIIRRLGVIAINTAIEADIYGNINSTNLFGTRMMNGIGGSGDFARNA
                     YLSIFTMPSAAKNGDISSIVPMVSHHDHTEHDVMILITENGVADLRGLSPKQRAMAII
                     NNCSHPYYRPMLKDYFLRSQKSGSLHTPHRLEEALSWHVRFLRYGTMKTNAADSVVVG
                     "
     gene            complement(2678..>3154)
                     /locus_tag="PLU28_09825"
     CDS             complement(2678..>3154)
                     /locus_tag="PLU28_09825"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HOG35396.1"
                     /translation="WFSDSYRSKEDWIKLYEDVQTFILPIVPNQYTRSKINYIFAPLI
                     NDELDFNSDLCVINGDINPYHIKFDKFKNKITGIIDLGTIGLGDPAYDLSVLMYNYGE
                     NFISMMSEVYPKLKYLIKRARFMRNTMEMQWLLNGLKLNEKSWFTLVLNKTFDDIL"
BASE COUNT         1089 a          480 c          629 g          956 t
ORIGIN      
        1 aaaatttttt atcaacatca tgttgatagt tgataaaaaa attaaaaaga gatgttgaag
       61 gtttttgaat gttcaaaagt tatgaacagt ttttgattat tcgaagttgt tatttagttt
      121 atcgacagtc catgttgata aatggttgct agtaatgttg gtaagttttt tattacagcg
      181 taaactctct ttttataatg cttacatact atgaacatat gttggtgttc gttgttaaaa
      241 agaatgttga ttgttgaaaa aaaaatacac ttctgccaca cttttctaag acctgcttaa
      301 ggaaaaagct ttattatgaa tgtaagcaca attagaagag aggtggaaaa tatgtacaga
      361 tatataggat atacaggtgg tatgacagga ataggtatgt tttttgtagt ggtagcagtt
      421 gttttactgg ttgtaatact ttcggtcgtt ctttataaag ttcttgcaaa taagagttca
      481 aaaaatgaaa aacatgaagt accaacgaag gaagaagata tagcgattga gcttttaaga
      541 caggagtatg caaaaggcag cataaatgaa gaagagtatg taaaaaggaa gacatttctt
      601 ataaacaact aagtatccgg cggactttgc cggatatttt ttgataaaac cgttacttta
      661 atatatgcag atagataata aaaaagatat aaaataacat ttgaaaaagt taatacttgc
      721 ttttcggtgc tgttttcaca cagcttaata gggaatccgg tcaaatgccg gagcggtccc
      781 gccactgtaa gaaggagtca gaatgcagta agccactgtt ttacgggaag gcgcattcag
      841 atgatgattt caagccagga gacctgccgg aaagaacaaa accactatga cttacgagga
      901 taaggaggtt ttaaaggtga gctgtaaaag tattttttat atatgctctt cttaaaaccc
      961 tttttctttt gaaaaagggt tttaacattt tatgtgtgtt gaatgaatgc ttaagttttt
     1021 tattaaaaca ctctttaaaa atcaaaggag agctaataat gataaaacag ttgataaaaa
     1081 acagttttgt tatgtcaaag gtgatgtcgg cttatgaggc ggcattaatg ataaaagacg
     1141 gaatgacagt tgcgaccagc ggttttaccc ccagtggcta tcctaaagaa gttcccaaag
     1201 cacttgcaga tattgtggaa agtacaggaa aaaaaataaa gcttactctt ttaacagggg
     1261 catcggtagg agaggaaata gacggcagat ggactcaggc aggaataata tgtaaaagaa
     1321 tgccttatca gaccaacaac cttttaagaa attcaataaa caggggagaa tgtgagtttt
     1381 cagatataca tctgagtaac tttgcccaga atataaggta tggattttat ggagaaattg
     1441 atattgccat aattgaagct gttggaataa ccgaagaagg ctttattatt ccgtcaactt
     1501 cagttggtgc gagccctgtt tatgtgcaaa aagcaaggaa ggtgattgta gagataaaca
     1561 catctcttag taaacaattg gaaggttttg ccgatgttta tatgcctgat gatcctccgc
     1621 acagacttcc tattccttta ttcagtgccc agcagagaat aggaacaaag tatattccat
     1681 gcaataaaga gaaaatagca gccgttgtta tttcagagat acaagataag catactgttt
     1741 tttcagagtg tgatgacaca gcattaaaaa tttccaatca tctgttgaat tttcttcata
     1801 acgaaataat aatgaacaga gtcccaaaaa ctctgctgcc tttacagtcc ggagtgggaa
     1861 atatagctaa tgctgtactt tccggtatag aaaaaagtaa tttttcaaat atctcctttt
     1921 atacggaagt aatacaggat tcgatgctaa agcttattaa aaaaggtaaa gctgtatttg
     1981 catcaggaac agctcttaca ctttcgactg actgtttcaa agagtttgaa aataatattt
     2041 cattttttaa ggaaagagtg gttttaagac cgcaggaaat aagcaacagt cctgaaataa
     2101 taagaagact tggggttata gctattaata ctgcgataga ggctgacata tacggtaata
     2161 taaactccac caatcttttc ggaacacgta tgatgaacgg aataggagga tcaggagatt
     2221 ttgcacgaaa tgcatatctt tctattttta ccatgccctc ggccgccaaa aacggggata
     2281 tatcttccat agtccctatg gtatcacatc atgaccatac agaacatgat gtgatgattt
     2341 tgattacaga aaatggcgtg gccgatctta gggggctttc acccaagcaa agggctatgg
     2401 ctataataaa caattgttct catccgtatt atagacctat gttaaaagac tattttttaa
     2461 ggtcacagaa gtccggatca ctgcatactc cacataggct tgaagaagct ctttcgtggc
     2521 atgtaagatt tctcagatac ggaacaatga aaaccaatgc tgcggacagc gttgtagtag
     2581 gatagaatac tttaaaaaga taaaatggtc ataaaaaatg gagtaaataa taaagccccg
     2641 aaaataaaat tcggggcctt atttaattat agacatatta tagaatatcg tcaaaagttt
     2701 tattgagaac caaagtaaac cagctttttt catttaactt taacccgtta agtagccact
     2761 gcatttccat ggtattgcgc ataaaacgag ctctttttat gagatattta agtttcggat
     2821 atacctctga catcatagaa atgaagtttt caccgtagtt gtacatcagg acagacaggt
     2881 catatgcagg atcacctaag ccaatggttc caagatctat gataccggtt attttatttt
     2941 taaatttgtc aaacttaata tgatatgggt ttatatcacc gtttattaca cacaggtctg
     3001 agttgaagtc aagctcgtca tttataagtg gagcaaagat atagtttatt tttgaacggg
     3061 tgtactggtt aggaactata ggaagaataa aagtctgaac atcctcatat aactttatcc
     3121 aatcttcttt tgaccggtat gaatccgaaa acca
//
LOCUS       DAOSHR010000225         3143 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_29083_length_3143_cov_7.036917, whole genome shotgun sequence.
ACCESSION   DAOSHR010000225 DAOSHR010000000
VERSION     DAOSHR010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 3143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3143
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29083_length_3143_cov_7.036917"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            276..704
                     /locus_tag="PLU28_09830"
     CDS             276..704
                     /locus_tag="PLU28_09830"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HOG35397.1"
                     /translation="MIRLMSVKDYEKVYALWTGTKGMGMRSLDDSQEGIKKFLNRNPN
                     TCFVYEEEGSIIGCILCGHDGRRAYIYHAAVAENYRRKGIGSLLLKQVYDSLEKEGIN
                     KAALVVFSNNTIGNSFWKNQGWEKREDLNYYNKVINPENK"
     gene            complement(812..1162)
                     /locus_tag="PLU28_09835"
     CDS             complement(812..1162)
                     /locus_tag="PLU28_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOG35398.1"
                     /translation="MAFFQRKIKKEETKSCSCGGKCDSESIDKAETVKAQEASVKILG
                     SGCIKCNKLEEATKEALKQLGMDTAVDHVTDFSKIAEYGVMSTPALVIDGKVVSCGKV
                     LKTEEVVKILQKFR"
     gene            complement(1205..2218)
                     /locus_tag="PLU28_09840"
     CDS             complement(1205..2218)
                     /locus_tag="PLU28_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOG35399.1"
                     /translation="MEVLKGLWDFFQNQILGMKWLNELIGNVLSTFGYDLNNRWIGSI
                     NFFIYDVIKIVILLCFLIFTISYIQSYFPPERSKKILGRFHGLGANIIAALLGTVTPF
                     CSCSSIPLFIGFTSAGLPLGVTFSFLISSPMVDLGSLVLLMSIFGPRVALVYVVVGII
                     IAVLGGTLIEKLHMEKYVEKFILNAAGVDIESPDLTVKDRLIYAKEQMGSTFKKVFPY
                     ILIGVGIGAVIHNWIPQEWISKVLGSHNPFGVIVATLIGVPVYADIFGTIPIAEALFS
                     KGAQLGVILSFMMAVTTLSLPSMIMLSKAVKPRLLGLFAAICTFGIIIVGYLFNIFQN
                     FII"
     gene            complement(2253..2579)
                     /locus_tag="PLU28_09845"
     CDS             complement(2253..2579)
                     /locus_tag="PLU28_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOG35400.1"
                     /translation="MKISNQKYAKIFKAFCEEQRLEILELLRSGEKCACKLLESIDIG
                     QSSLSYHMKILIESGIVESREEGKWTYYRISEKGSKNAIDILKELTTPNSADSNEETF
                     NCFCEK"
     gene            complement(2712..>3143)
                     /locus_tag="PLU28_09850"
     CDS             complement(2712..>3143)
                     /locus_tag="PLU28_09850"
                     /inference="COORDINATES: protein motif:HMM:NF024988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOG35401.1"
                     /translation="ICFALYVCSKEIIKKYRPLLEPLNLTYTSYIAMLSLWEKDGVTV
                     KELGDTLFLDSGTLTPLLKKLEITGYISRKRDTHDERNLRIYLTEKGKELKVRAEEIP
                     GNLLCSIYTGNYCDSSEMIKLRDTLHKLMRKFLASEKSDKT"
BASE COUNT         1023 a          603 c          481 g         1036 t
ORIGIN      
        1 ggcacatttt ttgtactttt ttcattttac cccttgactt ttaatagtta gtctccatac
       61 ttgtataata gcattatttt cctgtaagtt caatttgaaa ttctggtaat atttcatttt
      121 taatctgaga tacaccactt tcatgctgaa aagattaaaa aaacactggt tatgtctgaa
      181 acgcacaaaa atgaaagtta atgtggtatg atatcttttc gggggggcga attgatactt
      241 agtcttttat ttactattct acagggagga tgcatatgat tcgtcttatg tctgtgaaag
      301 attatgaaaa ggtttatgct ttatggacag gaacaaaggg aatgggaatg agaagtcttg
      361 atgattcaca ggaaggtatt aaaaaatttt tgaatcgaaa tccaaatact tgctttgtat
      421 atgaagaaga aggcagtatt ataggatgta tactttgcgg acatgacgga agacgcgcat
      481 atatatatca cgcagccgtg gcagaaaact atcgtagaaa aggtataggt agcttgcttc
      541 tcaaacaagt ttacgactct cttgaaaaag aaggcatcaa taaagcagct cttgtagttt
      601 tttcaaacaa cactatcggt aactcatttt ggaaaaacca gggttgggaa aaaagagaag
      661 acctcaacta ttataacaag gtaattaatc ccgaaaacaa gtagttattt taattaacag
      721 aatactgcgg cggtcaatct taatcaaaga taaccccgcc gcttgattca atctttaata
      781 cagcatcatc ttaaataagt atttataaca gttatctgaa cttctgcaga atttttacaa
      841 cttcttctgt ctttaggact ttaccgcaag ataccacttt cccgtctatg accagcgcag
      901 gtgtactcat aactccatac tctgctattt ttgagaaatc agttacatga tcaacagccg
      961 tatccattcc aagctgtttc aaagcctctt ttgtcgcttc ttcaagttta ttacacttaa
     1021 tgcatccact tcccagtatt tttacggatg cttcttgagc tttaacagtt tctgctttgt
     1081 caatactttc agaatcacat tttccgccgc agctacaaga tttagtttct tcctttttaa
     1141 tttttctttg aaaaaaagcc atagtaactc ctcctcatat aatatgacta ccgtttttta
     1201 gtttctatat tataaaattt tgaaatatgt tgaatagata accgacaatt atgattccaa
     1261 aggtgcagat tgctgcaaaa agacctaaaa gtctaggctt aactgctttg ctaagcatta
     1321 tcattgacgg taaagaaaga gtagtaactg ccatcataaa agaaagtata actccaagct
     1381 gtgctccttt agaaaataga gcttccgcta ttggtatggt accaaaaata tctgcataga
     1441 caggaacgcc tattaatgta gctactatca ctccaaatgg attatgactt ccaagaactt
     1501 tagaaatcca ctcttgtgga atccagttat gaataaccgc tcctatacca actcctataa
     1561 gtatataagg gaagaccttt ttgaaggttg aacccatctg ctcttttgca tagataagcc
     1621 tgtcctttac tgtcaaatcc ggagattcta tatctactcc agcagcattg agaataaact
     1681 tctcgacata cttttccata tggagctttt caataagagt acctccgagt acagctataa
     1741 taatacctac aacaacgtat actaatgcaa ccctcggtcc gaatatactc ataagaagaa
     1801 ccaaagagcc tagatccacc atgggagaag atataagaaa agaaaaagtc actcctaaag
     1861 gcagcccagc tgatgtaaat ccgataaaaa gaggaattga agaacatgag caaaaaggcg
     1921 taaccgttcc aagcaaggca gcgattatat tcgctcctaa tccgtgaaaa cgtcctaata
     1981 ttttcttact tctctcaggc ggaaagtagc tttgaatata acttatagta aaaatcaaaa
     2041 agcatagcag tataacaatt tttattacgt cataaataaa aaaatttatg ctacctatcc
     2101 aacgattatt taaatcatat ccaaaagttg acaaaacatt tcctattaat tcgttcagcc
     2161 atttcatgcc aagtatctga ttttgaaaaa aatcccataa acctttaagt acctccatgt
     2221 tctttcatcc tctcatatag attaattttt attcatttct cgcaaaaaca gttaaaagtt
     2281 tcttcatttg aatcagcact gttaggtgta gttaactctt tcaaaatatc aatagcgttc
     2341 ttacttccct tctcagaaat tctgtagtaa gtccatttac cctcttctct gctctcaaca
     2401 atacccgatt caataagtat cttcatatga tacgaaagag aggactgtcc gatatcaata
     2461 ctttcaagaa gtttacatgc acacttctcc ccacttcgaa gtaattccag tatttccagt
     2521 ctctgctctt cacagaaagc tttaaatatt ttcgcatact tttggtttga tattttcaaa
     2581 agatcacctc atatctattt tttttgatat gataaattat acttttcata tcgaaatttg
     2641 tcaatatgtc ctgtagttac tttaccataa gattcgctat aaataataaa aactatgcca
     2701 ttggcatagt tttaggtttt atcagatttc tcagaagcta aaaactttcg cataagctta
     2761 tgaagagtat ctctaagctt tatcatttca gagctgtcac agtaattccc cgtataaatt
     2821 gagcataaaa gattgccggg aatttcttca gctcttacct taagctcttt tcctttttct
     2881 gtcaaatata ttcttaaatt tctttcatca tgcgtatccc ttttcctgct tatataacca
     2941 gttatctcaa gctttttcag aagaggagta agtgttcctg aatcaagaaa cagtgtatct
     3001 ccaagctctt taacggtaac tccatccttt tcccataaac tgagcatggc aatgtatgag
     3061 gtatatgtaa gatttaatgg ttcaaggagg ggtctgtact tttttattat ttcttttgaa
     3121 catacatata aagcaaaaca aat
//
LOCUS       DAOSHR010000226         3128 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_29292_length_3128_cov_3.062154, whole genome shotgun sequence.
ACCESSION   DAOSHR010000226 DAOSHR010000000
VERSION     DAOSHR010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29292_length_3128_cov_3.062154"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="PLU28_09855"
     CDS             <1..415
                     /locus_tag="PLU28_09855"
                     /inference="COORDINATES: protein motif:HMM:NF013811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35402.1"
                     /translation="TAYVMEKTIEKAMEMENGFICTNVQETDLAGHKEDSFLYADRLS
                     VADKYISRLMPLLNPQDIMVVMADHGNDPTIGHSHHTRENVPILIYKKGVQNIFIGDR
                     KTLSDIGMTVADYFETEKPENGESFLKLLSKKEEC"
     gene            418..678
                     /locus_tag="PLU28_09860"
     CDS             418..678
                     /locus_tag="PLU28_09860"
                     /inference="COORDINATES: protein motif:HMM:TIGR01003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HOG35403.1"
                     /translation="MKSIEIILQNETGLHARPASEFVKLAKSFEAKITLVKGGKEYNG
                     KSILNVLGMGAGKGTQIELRAEGKDEEAALERLSSLIKAFSE"
     gene            687..2402
                     /gene="ptsP"
                     /locus_tag="PLU28_09865"
     CDS             687..2402
                     /gene="ptsP"
                     /locus_tag="PLU28_09865"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HOG35404.1"
                     /translation="MKATAVSPGIAIGKVFLLKKIEVESLQKYSDSMEKELSILETAL
                     EKSAFELQELIETSEAKLGKDDAEIFQAHLMILQDDEFVQTVRDHIESEKLCAAYAVM
                     KTADEFYIQFMNIEDEYIRERATDVKDVGMRVVKSILGTGTGLEAMPDGSVIVARELS
                     PSDTASMDSQKVCGIITEGGGRTSHAAIIARNLGIPAIVGATGITEQVETGSFIVFDG
                     ESGEVFIRPDEKIIQRYEKIKISRENYKKLLMQFKDKKTFTIDSHQVELYANIASQND
                     TSAALENGAQGVGLFRTEFLYMNRDSEPSEEEQLEVYKKVLLNMKSKPVIIRTLDIGG
                     DKDVPYLGLEKEDNPFLGFRAIRICLEKKEMFKKQLRAILRASVFGRARIMFPMISCL
                     EELRAAKQIVSECMQELDKKSIEYDRSIQTGIMIEVPSAAVISDILAEECDFFSIGTN
                     DLIQYTTAVDRMNHKISELYNQYNPAVLRLIKTVIENGHKKGIDVGMCGEAAGDEVMA
                     YLLLGLGLDEFSMSPPCILNIRRLFSLVGMNEARNDAQKVLEMKDAQEIKKFLEERNK
                     KIAGK"
     gene            complement(2452..>3128)
                     /locus_tag="PLU28_09870"
     CDS             complement(2452..>3128)
                     /locus_tag="PLU28_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG35405.1"
                     /translation="SFSSPHCTTTALIGPSGSGKTTITSLIARFWDTNKGKITLGGQD
                     IRDINPDSLLKHISMIFQEVYLLNDTVENNIKLGNPQATHEEVVQAAKLSRCHDFILQ
                     MDQGYKTVIGEGGSTLSGGEKQRISIARALLKDAPVILLDEATASLDADNEHEIRKAL
                     KKVTANKTVVVIAHRLNTIKDADQIIVLNEGRIEEAGSHAELMKNHKRYFNMFTEMEN
                     AQSFSL"
BASE COUNT         1018 a          565 c          706 g          839 t
ORIGIN      
        1 taccgcatat gtgatggaaa agaccataga aaaggcaatg gaaatggaaa atgggtttat
       61 atgtactaat gtacaggaaa cagatcttgc cgggcataaa gaagacagct ttctatatgc
      121 tgacaggctc agtgttgcag acaaatatat cagtaggctt atgccgctac ttaatccaca
      181 ggatatcatg gtagtcatgg cagatcatgg aaatgatccg acaataggac attcacacca
      241 tacaagagaa aatgttccga tactgatata caaaaaagga gtacagaaca tttttattgg
      301 agatcgtaaa accctttcag atataggaat gactgttgct gattactttg aaacagaaaa
      361 acctgaaaac ggggagtcct tcctgaaact tttatcaaaa aaagaggagt gctgagtatg
      421 aaatcaatag agataatctt acagaatgaa accggtctcc atgcaaggcc ggcatcggaa
      481 tttgttaagc ttgccaagag tttcgaggcc aaaataactt tggtaaaggg cggaaaagaa
      541 tacaacggca aaagcattct gaacgttttg ggaatgggag ctggcaaagg tactcaaata
      601 gagctccggg cagaaggaaa agatgaagaa gctgccttgg aaagattaag ctcactcata
      661 aaggctttta gcgagtaggt gataatatga aggccacggc agtttcacct ggtatagcca
      721 taggaaaagt attccttttg aaaaaaatag aagttgaaag tcttcaaaaa tactccgaca
      781 gtatggaaaa agaactctca atccttgaaa cggcacttga aaaaagtgct tttgagctgc
      841 aagagcttat agaaacttcc gaggcgaaac tcggaaagga tgacgcagaa atttttcagg
      901 cccatctgat gattttgcag gacgatgagt ttgttcagac tgtcagagac catattgaat
      961 ctgagaaact ctgcgcagcc tatgctgtaa tgaaaacagc ggatgagttt tatatacagt
     1021 ttatgaatat agaagatgag tacatacgtg aaagagctac cgatgtgaag gatgtcggaa
     1081 tgagagtggt gaaaagtatt cttggtacgg gaacggggct tgaagctatg cctgacggat
     1141 ctgtaatagt tgcccgtgag ctttcacctt cggacacagc gtctatggac agtcaaaaag
     1201 tctgcggcat aataacagaa ggcggaggca gaacctctca cgcagccata atagcaagga
     1261 atctcggaat tccggccatc gtaggtgcaa cagggataac agaacaggta gaaacaggat
     1321 ctttcatagt atttgacggt gaaagtgggg aggtttttat ccgcccggat gagaagatta
     1381 tacaaagata tgagaaaata aaaatttccc gggagaacta taaaaaactg ctaatgcagt
     1441 ttaaagataa aaaaactttt accattgaca gtcaccaggt tgagctttat gcaaatattg
     1501 cttcacagaa cgatacctcc gctgcacttg aaaacggtgc acagggagtc ggactgttcc
     1561 gaacagagtt tttatatatg aacagagaca gtgaaccctc tgaggaagaa cagcttgagg
     1621 tatataaaaa agtactttta aatatgaaat caaaacctgt cataataaga acccttgata
     1681 taggaggaga taaagatgtc ccgtatctcg gacttgaaaa agaggacaat ccttttctgg
     1741 gttttcgcgc cataagaatc tgccttgaaa aaaaggagat gtttaaaaaa cagctcaggg
     1801 cgatactcag ggcgagtgtt ttcggaaggg caagaataat gtttcccatg ataagctgtc
     1861 ttgaagaact aagggctgcc aagcaaatag taagcgaatg tatgcaagag cttgataaaa
     1921 aatctataga atacgaccgt tcaattcaga ccggaataat gattgaagta ccttcggctg
     1981 ctgtgatctc ggatattctg gctgaagaat gcgatttctt cagtataggt acaaacgatc
     2041 ttattcagta tacaacggcg gttgacagaa tgaatcataa aatatcggaa ctgtacaatc
     2101 agtataatcc agcagtgtta aggcttataa aaacagttat agaaaatggg cacaaaaaag
     2161 gaattgatgt gggaatgtgc ggggaagcag ccggagacga ggttatggct tatcttctcc
     2221 ttggacttgg tctcgatgaa ttttcaatgt ctcccccttg tatactcaat attcgccgtc
     2281 ttttctcact ggtcggtatg aatgaagccc gaaacgatgc tcaaaaagtt ttagaaatga
     2341 aggatgccca ggagattaaa aaatttcttg aagaaagaaa taaaaaaatt gcaggaaaat
     2401 agaaaaatct cacggaagtt ctttccgtga gatcttttta tggcaggggt actaaaggga
     2461 aaagctttgg gcattttcca tttcagtaaa catattaaag taccttttgt ggtttttcat
     2521 aagttcagca tgacttcctg cttcttctat ccggccttcg ttcagtacaa ttatctggtc
     2581 ggcatctttt atcgtattaa gcctgtgggc tataaccaca acggttttat ttgcagttac
     2641 ctttttcaga gcttttctta tttcgtgttc attgtctgca tcaagagaag ctgtagcttc
     2701 atcaagaaga atcaccggag catccttcag aagagcacgt gctattgaaa tcctctgctt
     2761 ttctccacca gaaagagtgg atccgccttc tcctataact gtcttgtatc cttgatccat
     2821 ctgtaaaatg aagtcatgac accttgaaag tttggctgcc tgaacaacct cttcatgtgt
     2881 agcttgagga ttgcccaact taatattatt ctcaacagta tcgttcagca ggtatacttc
     2941 ctggaagatc atacttatat gctttaaaag agagtccgga tttatatctc ttatatcctg
     3001 ccctcctaga gttatttttc ccttgtttgt atcccagaat cgtgcaataa gacttgtgat
     3061 ggttgttttt cccgaccctg acggccctat aagtgcagtt gtcgtgcagt gtggggaaga
     3121 aaagctta
//
LOCUS       DAOSHR010000227         3101 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_29656_length_3101_cov_3.662837, whole genome shotgun sequence.
ACCESSION   DAOSHR010000227 DAOSHR010000000
VERSION     DAOSHR010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29656_length_3101_cov_3.662837"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2195)
                     /gene="lon"
                     /locus_tag="PLU28_09875"
     CDS             complement(<1..2195)
                     /gene="lon"
                     /locus_tag="PLU28_09875"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOG35406.1"
                     /translation="MEEKGTYIEKYIDEITKSAIPENMPCIPTRTKGLLFPNTVLPMF
                     IGRDKSLLALEKSLEAFNSYIFVTSQKVLENENPSIEEIYHTGTVGRVVQISKTPNGE
                     YKILLEGIKRGRIKKVIDDKSFFLFDIEVLEDKFKKTKKLEALVRKVKALFQKYMELT
                     KKFPPEAILAIDETSEPDVISDLVASVLPIELEEKQGLIESYQTQKRLEDELEILTRE
                     IELLELEENLESKVKEKIEKTQKEFYLREKLKTIQQELDGGTDEEISELKQMLLNKEY
                     PKFVKEKAEKEIEKLSKMSPYSPESTVVRNYLDWILNMPWNTETKDSIDIRMAKKILD
                     SNHYGLSEPKERIIEFLAVKKLSDLARTPILCFVGAPGVGKTTLGKSIADSLGRKFGR
                     ISLGGCRDEAEIKGHRKTYVGAMPGRIIQTIRKLEVNNPVIVLDEIDKMGISYQGDPA
                     AALLEVLDPGQNATFSDNYLEIPFDLSKVIFVATANVVHTIPPALLDRMELLEIAGYT
                     DFEKYNIAKNHIIKNVLPQNGLKAEDVQLTPNALKTLISMYTREAGVRDLERTVSRLM
                     RKVAVKYVKNRKKVVVKEEELKEYLGTPPFFESEKNGRSESGLVTGLAWTAYGGSILN
                     VEALTFPGKGNLILTGKLGEVMQESAKISLSLCRRILSSNNIVDEKYFEKNDFHIHLP
                     EGAVPKDGPSAGVTLTTALVSAISNKKVRNDIAMTGEISLRGKVLAVGG"
     gene            2441..>3101
                     /locus_tag="PLU28_09880"
     CDS             2441..>3101
                     /locus_tag="PLU28_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HOG35407.1"
                     /translation="MNIVQLQNITFLTGKKLDILEHIQECIQQNKKVWIMTVNALMFM
                     EYLKNDIYTQAIKKASFSVPDGIGVVKYLKKNGYKTDRCPGIETMDFLCKLASHKKYT
                     VYLLGSRGDTVSIAAQKLMEAYGITVAGFHDGYFSSSEETDIVNDINSKNTDMVFVGM
                     GIPKQEEFILRNFDYLNVKAIMGVGGSIDVFAGQVKRAPIFFQKLGMEWLFRMLEDPK
                     RF"
BASE COUNT          903 a          600 c          513 g         1085 t
ORIGIN      
        1 atacctccaa ctgcaagcac ttttcctctg agacttatct ctcctgtcat tgctatatca
       61 tttctgactt ttttattact tattgcagaa accaaggctg ttgtaagtgt aactccggcc
      121 gaaggaccat ccttgggaac agctccttca ggaagatgaa tatgaaagtc attcttttca
      181 aagtactttt catctacgat attattactg cttaaaattc tacggcatag gctaagagaa
      241 attttagctg attcctgcat cacttctccc agtttacctg taagaataag atttccttta
      301 ccaggaaaag taagtgcttc aacgtttaaa atgcttccgc cgtaagcagt ccacgcaagg
      361 cctgtaacaa gtccgctttc agatcttccg tttttttcgg attcaaaaaa tggaggagta
      421 cccagatact ctttaagttc ttcttccttg actactactt tttttcgatt tttaacgtac
      481 tttacggcta cttttctcat cagccgcgaa actgttcttt ccagatccct tactccggct
      541 tctctagtgt acattgatat tagagtttta agtgcgttag gggttagctg tacatcctct
      601 gctttaagcc cgttttgagg gaggacattt tttattatat gattctttgc aatattatat
      661 ttttcaaaat ctgtataacc tgcaatttca agaagttcca ttctatcaag caaagctggt
      721 ggaattgtat gtacaacatt ggcagtagct acaaagatta cctttgaaag gtcaaaagga
      781 atctcaagat agttatcaga aaaggttgca ttctgacctg ggtcaagaac ttccagaagt
      841 gctgcagcag gatcaccctg ataagaaata cccatcttat ctatttcatc taatactatc
      901 actggattgt taacttccaa cttccttata gtttggatta tcctaccagg catagctcct
      961 acataagttt ttctgtgccc ttttatctct gcttcatctc tgcatcctcc aagcgatatt
     1021 cttccaaact tcctgcccag cgaatcagct atggactttc ccaaagttgt tttcccgact
     1081 cctggcgctc caacaaaaca aagaattgga gttcttgcca gatcagaaag cttctttact
     1141 gccagaaatt ctattattct ttcctttggt tcagaaagac cataatgatt cgaatccagg
     1201 attttttttg ccatccttat atctattgaa tctttggtct cagtattcca aggcatatta
     1261 agtatccaat caagataatt ccttacaaca gttgattcag gtgagtatgg ggacatcttt
     1321 gaaagcttct ctatttcttt ttctgccttt tctttaacaa actttggata ctccttattc
     1381 aaaagcattt gcttaagttc ggagatctct tcatctgtac ctccatcaag ctcttgctgt
     1441 atagttttta atttttcccg aagataaaat tccttttgag tcttctctat cttctctttt
     1501 acttttgatt ctaaattttc ctcaagttca agaagttcta tttctctagt cagtatttca
     1561 agctcatcct cgagtctttt ctgagtctga taactttcta taagtccctg tttttcctca
     1621 agctctatcg gaagcacaga agcaaccaga tcgcttataa cgtcaggttc actggtctca
     1681 tctattgcaa gtattgcttc aggaggaaat ttttttgtaa gctccatata tttctggaaa
     1741 agagctttta cttttcgtac aagtgcctca agctttttag tttttttgaa cttatcctct
     1801 aaaacttcta tatcaaaaag aaaaaaagat ttatcatcaa tgaccttctt tatacgacca
     1861 cgctttattc cctcaagaag aattttatac tcaccgttcg gtgttttaga aatttgaaca
     1921 accctgccta ctgtcccggt atgatatatt tcttctatcg aaggattctc attttccaag
     1981 accttctgag atgtcacaaa tatatagctg ttgaaggctt caagagactt ttccaatgca
     2041 agaagcgatt tatctcttcc tataaacatt ggcagaactg tattaggaaa caaaagccct
     2101 ttggtacgtg ttggaataca tggcatattt tcaggaatcg cagacttagt tatctcatca
     2161 atatattttt caatataagt acctttttct tccataaaat cactctccac cactcatttt
     2221 ctttgataac aattttatca atttgatatt tagataagat gtcggaagtg aaaatagaat
     2281 tatctgtaat acttccaacg tattgtaaac aagagttact tttaaattaa tttagagttc
     2341 ggatatataa aaaagtgtta gaatattaag tgataataaa tttatgatta ctttgtgtta
     2401 gcttatactt tttttagatt actgaggtga tttttcataa atgaatatag ttcagttgca
     2461 gaatataacc tttttaaccg gtaagaagtt ggatatactt gagcatattc aggaatgtat
     2521 ccagcagaat aaaaaagttt ggataatgac cgttaatgca cttatgttta tggaatatct
     2581 taaaaacgat atatacaccc aggctattaa aaaagcttct ttttctgtac ctgatggaat
     2641 aggtgtggtt aagtacctta aaaaaaacgg atacaaaacc gatcgctgtc cgggaataga
     2701 aactatggat tttttatgca aacttgcctc tcataaaaaa tatactgtat accttcttgg
     2761 aagccgtgga gatactgttt ccatcgcagc acagaaactc atggaagcgt atggaattac
     2821 tgttgcggga tttcatgatg gttatttttc ttcttcggaa gaaactgata tcgtcaatga
     2881 tataaattcg aaaaatactg acatggtctt tgtaggtatg ggaataccca aacaggaaga
     2941 gtttattcta cgaaacttcg attatttaaa cgtaaaggct ataatggggg tgggaggaag
     3001 tattgatgtt tttgccggac aagttaagcg tgctccgata ttcttccaga agcttggaat
     3061 ggaatggctt ttcagaatgc ttgaagatcc aaaaagattt a
//
LOCUS       DAOSHR010000228         3091 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_29794_length_3091_cov_4.951252, whole genome shotgun sequence.
ACCESSION   DAOSHR010000228 DAOSHR010000000
VERSION     DAOSHR010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 3091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3091
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29794_length_3091_cov_4.951252"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="PLU28_09885"
     CDS             <1..409
                     /locus_tag="PLU28_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35408.1"
                     /translation="VFYRVWYPRAETERYFKFLGYLKVTVDFSVLNLLKYTLSFILTI
                     SFILLGLTLSIVNIYNSRNIVRPFKRIMDEIDQIADGKREKEINDSEYVSIKEYQKAM
                     ETVKKIEDKISSQIIDSNSLQRYVKTLKKDKDA"
     gene            746..2614
                     /locus_tag="PLU28_09890"
     CDS             746..2614
                     /locus_tag="PLU28_09890"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOG35409.1"
                     /translation="MSEYLLLDNLQKSSAFESFITPLMKTTGFITTDSELISSLEDKS
                     KREVVVGVFSDLQKKFPEIQLAYAGYPDKAFVSYPVVDLGKDYDPTIRVWYTQAKSSP
                     KNVILTDPYIDAVTKLMMVSITKAVVDPSSNDVVAVFGVDFSLDALKDILFKESSYKT
                     AYSFVVNEAGVTVIHKDNSLVGKSVKDQEFFIKASSSSGYINYTSDKKKYLAFYEKLP
                     STKWVVYTVIEEAEVLSEAKKSLIIMLILSGSIIAVAIVIALIFVKTLIAKPIAAIVK
                     DIAVFGKGDLTVRFSEKGGSEIEDMAKSLNSMAGSLGESMKSIQEASQQINSSAGDLA
                     SVSEETSATSEELSSQAESIQNNVQNTSASIEELSSSVDEVATSAQNVSKASLELAKS
                     AQQVSETVNSGLKDIKGISDIIKEAVVQTDNTAALVTALATQSVNVGKIVDSINSITE
                     QTNLLALNAAIEAARAGEAGKGFAVVADEIRKLAEESKKATQNITTILTDVQQKASQA
                     DLATKQTKSIVEKVNTGGNNISVQFTEIAKQVDNITSMTENVSASAQQQSAAAQEITS
                     AMENASKMTTSIYEEIKNVVIAINQQSEAAQQVSANGEELNALAEGLESQVKKFKV"
     gene            complement(2639..>3091)
                     /locus_tag="PLU28_09895"
     CDS             complement(2639..>3091)
                     /locus_tag="PLU28_09895"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOG35410.1"
                     /translation="KELIKNNGVGIVHCPQSNFNLSSGIMPAAEYLNNGINVFLGSDI
                     AAGHSMYIPDIIVSAVQASKIIHMKNPQYMPLTLAQVFYMASTGPENLFPGSGKLQKG
                     MSFDALIIDDERKDGTVEERLQRFIYTGGKEHIKERFLRGISLKKSRV"
BASE COUNT         1017 a          543 c          654 g          877 t
ORIGIN      
        1 ggtattctac agggtatggt atccccgtgc agaaacggaa agatacttta aatttctggg
       61 atatctgaag gttacagttg atttctcggt tctgaacctt ctcaagtaca cgctgagttt
      121 tattcttact ataagcttta tacttcttgg tcttacgctt agtattgtta atatttacaa
      181 ttcaaggaat attgtaaggc cttttaaaag aataatggat gagatagacc agattgctga
      241 tggaaagaga gaaaaagaaa taaatgacag tgagtatgta agtattaagg agtatcaaaa
      301 ggctatggag acagtaaaaa agattgagga taaaatttct tcacagataa ttgactcaaa
      361 ctctcttcaa agatatgtaa aaacattgaa aaaggataaa gatgcctgaa agggcatctt
      421 tttttatgga accgatagta ggtatgtatt taattaatca gattttacgt aactgtcgaa
      481 ttattgagta tatttaatat gttctgttaa ccattatgta agtttttatt attttaaata
      541 ccctagttgt tgcatttcaa aagtaaaaaa tgtataattg ataaaaagaa ttaattatag
      601 gttaattata ttaagaaagg ggtgagaata tgaaaaaagc ataggcggtc ttatcattct
      661 cctgatcctt ttggtatcag ctcttcctct tggagtgctg actgcgtata caagcataaa
      721 ctcctacagt aaggatttca aatcggtgag tgagtatctt ttgctggata atcttcaaaa
      781 gtcatcagca tttgaatctt ttatcactcc tttgatgaaa actacgggct ttataactac
      841 agacagtgaa cttataagct ctcttgaaga taaaagcaag cgtgaagtgg tggtaggagt
      901 tttttctgat cttcagaaaa aatttcccga aatacagctt gcatatgccg gttatcccga
      961 taaagctttt gtctcttatc cagtagtgga tctgggaaaa gactatgatc ccacaataag
     1021 agtatggtat acacaggcta agtcatcacc caaaaacgtt atactcactg atccttatat
     1081 tgatgccgtt accaagctta tgatggtttc aataaccaaa gcagttgtag atccttcttc
     1141 caatgatgtg gtagcggttt ttggagtgga cttttctctg gatgccctta aagatattct
     1201 ttttaaggag agttcttata aaactgcgta ttcctttgtt gtaaacgaag ctggagtaac
     1261 agtcatacac aaggataact ctttggtagg aaaaagcgtg aaagatcagg aattttttat
     1321 taaagcatcc tccagcagcg gatatataaa ctacaccagt gataaaaaga agtatcttgc
     1381 attttatgaa aagcttccaa gtactaaatg ggttgtctat acggtaattg aagaagcaga
     1441 ggttctgagc gaagccaaaa aatccctgat aataatgctc atactttcag gatcaataat
     1501 agcagttgca atagtaatag cactgatatt tgtaaaaacc ttgatagcta agcctatagc
     1561 cgctatagtt aaagatatag cagtgtttgg aaaaggggat cttactgtac ggttttcaga
     1621 aaaaggcggt tctgaaatag aagatatggc aaaaagcctc aacagtatgg caggttcatt
     1681 aggggaatca atgaagtcca tacaagaagc ttcccagcag ataaactctt ctgccggaga
     1741 tcttgcaagc gtttccgagg aaaccagtgc tacaagcgag gagctttctt ctcaggccga
     1801 aagtatacaa aataatgtac agaatacatc ggcctctata gaagagttaa gttcatcggt
     1861 agatgaagtt gcaacaagcg ctcagaatgt ctccaaagcc tctcttgagc ttgccaagag
     1921 tgcccagcag gtaagcgaga ctgtaaatag cgggctgaag gatataaagg gtatttcgga
     1981 tataataaag gaagcggttg ttcagacaga taatactgca gctcttgtaa cggctctagc
     2041 cacgcagtct gtcaacgtcg gaaaaatagt cgattccata aacagcataa ccgaacagac
     2101 caaccttctg gcattgaatg cagctattga agcagccaga gcgggtgagg cgggaaaagg
     2161 ttttgctgtg gttgccgatg agataagaaa actggcggag gaaagtaaaa aagctaccca
     2221 gaatattacc acgatactta cagatgtcca gcagaaagct tcgcaggcag atttggcaac
     2281 caaacagacc aaaagtatag ttgaaaaggt aaacaccgga ggaaacaaca taagtgttca
     2341 gtttaccgaa attgccaaac aggtggacaa tatcacctca atgaccgaaa atgtttctgc
     2401 cagcgcccaa cagcagagtg cggcagccca ggagattact tcggcgatgg aaaatgcctc
     2461 aaagatgact acgagcattt acgaagaaat aaaaaatgtg gtgatagcaa ttaatcagca
     2521 gagtgaggca gcgcagcagg taagtgctaa cggagaagaa cttaatgctc ttgccgaggg
     2581 tcttgagtca caggttaaaa aattcaaggt ataatataaa aaaagagcgg ggtgttttct
     2641 aaaccctgct ctttttaagt gatatgccac ggaggaatct ttcctttata tgttcttttc
     2701 ctccggtata tataaacctc tgaagtcttt cttcgacagt tccatcttta cgttcatcat
     2761 ctataatcag ggcatcgaag gacatacctt tttgaagctt tcctgagccg gggaatagat
     2821 tttccggtcc ggtgcttgcc atataaaaaa cctgcgctaa agttaagggc atatactgcg
     2881 gatttttcat gtgaattatc ttggaagcct gaactgcact tacgattata tcaggaatat
     2941 acatgctgtg gcctgcggca atatcactcc ctaaaaaaac attgattcca ttattcaagt
     3001 actcagcagc aggcattata ccgcttgaga gattgaagtt agactgaggg cagtgcacta
     3061 tacccactcc attatttttt ataagctctt t
//
LOCUS       DAOSHR010000229         3087 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_29848_length_3087_cov_5.326847, whole genome shotgun sequence.
ACCESSION   DAOSHR010000229 DAOSHR010000000
VERSION     DAOSHR010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29848_length_3087_cov_5.326847"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            106..1359
                     /locus_tag="PLU28_09900"
     CDS             106..1359
                     /locus_tag="PLU28_09900"
                     /inference="COORDINATES: protein motif:HMM:TIGR03986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03986 family CRISPR-associated RAMP protein"
                     /protein_id="HOG35411.1"
                     /translation="MYNKHHESVFVLKKDDIIRVTEEDVENLRKTLKLYNDENINLKL
                     KEERKQDKGRKWYKEYEVKEGALVYYNKERDKDKDKDKDKDKDKDKDKDKDGIYMTPA
                     CISKEVFNNQLKQILKENGGYNPCSSKEVCPACALFGMIANDGKENTAISSRLRFTDA
                     QAELKSNKNEYYDKITILEELASPKVSATEFYMEEPSIRPDLWNYDYAIKWKGKIPER
                     VKDYKPRIRGRKFYWHSKKVLTSNETTERNSTVRPVRAGVTFTFKVYFDRINEEELKK
                     LIWTLNLGSNENTNCHKMGKGKPIGLGSVKITVDNVMERTVEIKNNTLLRTETKSTVD
                     LENAVDVNSTNIKEILKITDFSKAPNNVRYPMGYTKKDGKNGVGPHQWFIANKSFSNP
                     PMNPKINRTLPKITDSKTTLNDYEM"
     gene            1421..2554
                     /locus_tag="PLU28_09905"
     CDS             1421..2554
                     /locus_tag="PLU28_09905"
                     /inference="COORDINATES: protein motif:HMM:TIGR02619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative CRISPR-associated protein"
                     /protein_id="HOG35412.1"
                     /translation="MKDTMICTVGASTIGNIKRNELLNALYEKEKYEEIAKNFLNQPG
                     QPQDIQLLGAEINSVASIVDLGHIDETKNIYFLHSQTPEGHSMAKILKYYFTNSPSLN
                     FKNVQTIEIEGLSDSNKEDFKRKGLKNLVITMSRISRNHYAKTIINATGGYKAQIAFA
                     LALGQGMQIPVYYRYEKFPSVIELPPLPLTLDFQLYLNHRKIFDIIQDTEEISGFTLF
                     SDVESIYNALEDKVKLLFEIEKMENKKYITINAMGLTFIESAKTYYKMQKKDITLKPR
                     QNKDIQFLSSESEKNSLGLIKKYRIQEILNQVPYIELARVRKYSKIEINGGVVVDTLD
                     KDLKVSIAKTEGVIRFLIETTATTPEELERAKNELQEYLNNKL"
     gene            2659..3021
                     /locus_tag="PLU28_09910"
     CDS             2659..3021
                     /locus_tag="PLU28_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35413.1"
                     /translation="MKKILIILLAVLTTSMTMLAQDQYLDKDMNVNIGMIQWAERNSE
                     GQITGYKGFNLGMGLSVTKYFDPLEVGKATPFWHWGTVMLLVPYIGVGIHYQVNEVFY
                     LELLTFYLVPTLQIGLTF"
BASE COUNT         1277 a          574 c          548 g          688 t
ORIGIN      
        1 tttcacttgc attatctaac caactttttc ttaaaaatcc aaaataatac atcaaagagc
       61 aatgataatc cgattattgt cggtcacgtt cacatatgag aagagatata taacaaacac
      121 catgaatccg tatttgtttt aaagaaagat gatattataa gagtaacaga agaagacgtt
      181 gaaaacctca gaaaaacact gaaactctac aatgatgaaa acataaattt aaaactaaag
      241 gaagaaagaa agcaggataa aggaagaaaa tggtataaag aatatgaagt aaaagaagga
      301 gctctagtat actacaataa agaaagagac aaagacaaag acaaagacaa agataaagat
      361 aaagataaag acaaagataa agataaagac ggaatctaca tgaccccagc ttgcataagc
      421 aaagaagttt ttaacaacca gcttaaacag atcctaaaag aaaacggcgg atacaacccc
      481 tgcagctcaa aagaagtatg cccggcatgc gccctgtttg gaatgatagc caatgatgga
      541 aaagaaaaca ccgctatctc ctcacgcctg agatttacag atgcacaagc cgaactaaag
      601 tccaataaaa acgaatacta cgataaaata accatacttg aagagcttgc atcaccaaaa
      661 gtatccgcaa cagagttcta tatggaagaa ccctctattc gccctgatct gtggaactat
      721 gactatgcca taaaatggaa ggggaaaatt ccggaaagag taaaagatta caaacccagg
      781 ataagaggca gaaaattcta ctggcactcc aaaaaagttc ttacatcaaa tgaaaccaca
      841 gaaagaaaca gcactgtcag acctgtgaga gcaggagtaa cctttacatt taaagtatac
      901 tttgaccgta tcaacgaaga agaactcaaa aaactcatat ggacattgaa cttaggatca
      961 aacgaaaata ccaactgcca taaaatggga aaaggaaagc ccatcggttt gggaagcgta
     1021 aaaataacag tagataatgt aatggaaaga accgtggaga taaaaaataa tacccttctc
     1081 cgcacagaaa ccaaaagtac cgtagaccta gaaaatgcag tagacgtaaa ttcaaccaac
     1141 ataaaagaga tccttaaaat aaccgatttc tcaaaagctc cgaacaatgt cagatatccc
     1201 atgggataca ccaaaaaaga cgggaagaac ggagtaggac cacatcagtg gtttatcgcc
     1261 aataaaagct tttcaaatcc accaatgaac ccgaaaataa ataggactct gcccaaaatt
     1321 acggattcaa aaaccactct gaacgactac gaaatgtgaa aaagcctccg atgagcttta
     1381 aacagctcat acaaagtata tccaaaggat ggtgaaataa ttgaaggata ccatgatatg
     1441 cacagtagga gcaagcacaa taggaaatat aaaaagaaat gaactgctta atgcgttgta
     1501 cgaaaaagaa aaatacgaag aaatagctaa aaattttctt aaccagcccg gacagccaca
     1561 agatattcag cttctgggag ccgaaataaa ctcggttgca agcatagtcg accttggaca
     1621 tatagacgaa accaaaaata tctactttct ccactcacaa acacccgaag gtcattcaat
     1681 ggcaaaaatt ttaaaatact actttaccaa ctcaccatcc ttgaacttta aaaatgttca
     1741 aaccatagag atcgaaggac tgtcagattc aaacaaagaa gatttcaaac gcaaaggact
     1801 taaaaatctt gtaataacca tgtcacggat atccaggaac cattacgcaa aaaccataat
     1861 aaatgcaacc ggaggataca aagcccagat agcgtttgca ctggcgctgg gtcaaggcat
     1921 gcagataccc gtttactacc ggtacgaaaa gtttccgtca gttatagagc tgccgccttt
     1981 accactcaca ctggattttc aactctatct gaaccacaga aaaatatttg atatcataca
     2041 agatactgaa gaaatctcag gatttacact tttcagcgat gtagaaagca tttacaacgc
     2101 acttgaagac aaagtaaagc ttctctttga aatagaaaag atggaaaaca aaaagtacat
     2161 taccataaac gccatgggac tgacctttat agaatctgcc aaaacatact acaaaatgca
     2221 aaagaaagat ataacactaa aaccaagaca aaacaaagac atacagtttt taagcagtga
     2281 aagtgaaaaa aactcccttg gacttataaa aaaatacaga atacaggaaa tactgaatca
     2341 agttccatac atagagctag cacgagtaag aaagtactca aaaattgaga taaacggagg
     2401 agtagttgta gacaccctgg acaaagatct gaaagtttca atagccaaga cggaaggagt
     2461 aataagattt cttattgaaa caactgccac aaccccggaa gaactggaaa gagctaaaaa
     2521 tgaacttcaa gaatatctga ataacaaact gtaaaaaaac taaccgcagg agataatcct
     2581 gcggtttctt attacctttt tttaaatgtc gaataataac ttgaggaaga gaacaaccat
     2641 taaaaaggag aagaaatcat gaagaagata ctgattattt tactggcagt actgacaaca
     2701 agcatgacta tgcttgctca agaccaatat cttgataaag atatgaatgt caatattgga
     2761 atgattcagt gggcagaaag aaattcagaa ggacagataa ccggttacaa aggatttaat
     2821 cttggcatgg gattatctgt aacaaagtac ttcgatccgt tggaagtagg aaaagctact
     2881 ccattttggc attggggaac tgtaatgctt ttggttccat atatcggtgt aggaatacac
     2941 tatcaggtaa atgaagtctt ctaccttgaa cttctgacat tttatctggt tccaacgttg
     3001 caaataggat taacttttta agaaacccct ttaaaaacaa ataagcattc ggcgtcaaaa
     3061 acggatactt aaatatatta aactaaa
//
LOCUS       DAOSHR010000230         3079 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_29945_length_3079_cov_7.753307, whole genome shotgun sequence.
ACCESSION   DAOSHR010000230 DAOSHR010000000
VERSION     DAOSHR010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3079
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29945_length_3079_cov_7.753307"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1274)
                     /locus_tag="PLU28_09915"
     CDS             complement(<1..1274)
                     /locus_tag="PLU28_09915"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HOG35414.1"
                     /translation="MNNDFNESSELTLQDILRVILRRKWLIFWIVFIAGILSVIYALS
                     LPKIYEAQMYLEFKDTAMQQYGSLTSSALAGLTGGSSFSASPENEIQKMKNREVLRKV
                     VQKLELVEIFNDGKLDDKKSITELENEVIGDISEELSIQNMKNTSIIKLSYQYKDKKL
                     VSEIIHWVYYYYEEFNRQLTNSDYNEFLMAYSPMFTDISAQYEEINKKVIDYELKNKV
                     SSDFNAISPLFDAYVTNYSGVLGIDKSKKDLEISIKAFEKQYLEVDDKFKEVLSTETN
                     PNLQSIKNQIITSKIELESVKVLYPNSGKIGEIETKISTLQQNFNEILKNILSNNLYY
                     LSNIDPSLLQKYIDMNIQYQTLDMVKLSSEELLKKFDKEIADKSPVLYDYFLLKRDQS
                     VLRSQYEYLRTSIEQARLKDTTYQSKFRILQQA"
     gene            complement(1287..>3079)
                     /locus_tag="PLU28_09920"
     CDS             complement(1287..>3079)
                     /locus_tag="PLU28_09920"
                     /inference="COORDINATES: protein motif:HMM:NF022007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HOG35415.1"
                     /translation="DFGLKKGDYLFITPSKNNVYVYSDFATKVLEYTDMMTLKDVFNS
                     LGVTYEEVDDEISILRGDNLLKFSLSDIIFGLKADGKDFKIEKSDTVFFKRYEKYVFA
                     MINQNVTKVDFKKKDEFTVLMLLSKLAMNPKAVEYIKCEEKELSVSDTLTAKAYIIIE
                     PLKNTVYLNGSFKSTGKISFDFDESLTLARIFSVAGFKEDFSGRLMVINSGGESFEYR
                     IDAKDMSKYIDITVEPQSVLVAFPGERIIYSVGEVADVIAYKDGDTLYDIAMRYKLDE
                     SYEIRYSFEATQSTVNAGNADLLKSIELSGKAFMEVIKKVSDYVIVYKDGNSKTFEAK
                     KPVKLIEAFDSMQGFSPSDRGVIKVFTGDTISATYTNYDIINHPLTVVPNGSYVVIQP
                     DVKYSYINVFGSVGFNSIRTDIPISLVEILARSGNNWSNQYEVVIYRPDDTRERIKIE
                     DIDLLSRTFVEPGSIVYLPTYDKQIIYMLGQVGAPGVVQYVKGMTLFDALIRAGGVRK
                     DGEPANVFLFKDGPDNPPITLDVSPLFRNSVSLKSSMNPELKPGDIVYVPKSLMTNVV
                     EAMSTVTTFLEFINSTTNSVYNLVNIFGKK"
BASE COUNT          904 a          647 c          515 g         1013 t
ORIGIN      
        1 gcctgctgaa gaatcctgaa cttggactgg tacgtagtat ccttaagcct ggcctgttca
       61 atagaggttc tgagatactc atactgactt cttagaacac tctggtcacg cttgagtaaa
      121 aaataatcgt aaagaacagg agatttatct gctatctcct tgtcaaactt ttttagcagc
      181 tcctctgagc tgagttttac catatcaagc gtctggtact gtatgttcat atcaatatac
      241 ttttgaagca aggacggatc tatattggaa agatagtaca ggttgtttga taggatattt
      301 ttcagaatct cattgaaatt ttgctgcaaa gtacttatct tggtctctat ttcgcctatt
      361 ttgccagagt taggataaag caccttaacg ctttcaagct caattttgga ggttattatc
      421 tgatttttta tactctgtag attaggattg gtttctgtac ttaaaacctc tttaaacttg
      481 tcatccactt caagatactg tttctcaaag gcttttatgg atatttcaag atccttttta
      541 gacttatcta tccccagtac gccggagtag ttggtaacat acgcatcaaa caaaggagaa
      601 atggcgttaa aatcactgga taccttgttt ttaagctcgt aatctattac ttttttgttt
      661 atctcctcat actgagcgga tatatctgta aacatcggac tgtaagccat taaaaattcg
      721 ttgtagtcac tgttggtaag ctgacggttg aactcttcgt agtagtagta aacccagtgg
      781 attatctcag acaccagctt cttatctttg tactgatagc tcagcttaat tatagatgta
      841 tttttcatgt tttggattga aagttcttct gaaatatctc ctattacctc attctcaagc
      901 tctgttatac tttttttatc gtcaagcttt ccatcattaa aaatttctac aagctcaagc
      961 ttttgcacta ccttgcgcag aacctccctg ttcttcatct tctgaatctc attctcagga
     1021 gacgcactga aagaagaacc tcccgtcaga ccggcaagag cacttgaagt aagagaaccg
     1081 tactgctgca tggcagtgtc tttaaattca agatacattt gggcttcata tattttaggc
     1141 aagcttaatg catatattac agataatatt ccggcaataa aaacaatcca gaatataagc
     1201 cacttccttc ttaatatcac ccgtaaaata tcctgcaagg taagttctga gctttcatta
     1261 aaatcgttat tcataagcta atccccctat ttcttaccaa aaatattgac tagattgtag
     1321 acagagttgg tagtagagtt tatgaactca agaaatgtgg ttacagtaga catagcttcc
     1381 actacattag tcatgaggct cttgggtaca tatactatat ctccgggctt aagctcgggg
     1441 ttcatactgc tcttaaggct cacgctgtta cggaaaagag gtgaaacgtc aagagtaatc
     1501 ggcggattat ccggcccgtc tttaaaaaga aatacatttg caggctctcc gtccttcctg
     1561 actccccctg ctcttatcag agcatcaaaa agtgtcatac cttttacata ctgtacaaca
     1621 cccggagcac ctacctgtcc gagcatatat attatctgtt tatcgtatgt tggcagatat
     1681 actattgacc caggctccac aaaagttctg cttaaaagat ctatgtcttc tatttttatt
     1741 ctctctctgg tatcgtccgg acgatagata accacttcgt actggtttga ccagttgttg
     1801 cctgaacgcg caagtatttc aaccaatgaa atcggaatat ccgttcttat actgttaaat
     1861 ccaacacttc caaaaacatt tatatagctg tactttacat ccggctggat aaccacataa
     1921 gaaccatttg gaacaaccgt gagaggatgg tttattatat cgtagttcgt atatgttgca
     1981 gaaattgtat ctcctgtaaa taccttgata actcccctgt ctgaaggaga aaagccctgc
     2041 atggaatcaa aagcttctat aagctttaca ggttttttgg cttcaaatgt ctttgagtta
     2101 ccatctttat atacgataac gtaatcagat acttttttta ttacctccat gaatgcctta
     2161 ccgcttaact cgatactctt aagaaggtca gcattacctg cattaaccgt actttgagta
     2221 gcttcaaaag agtaccgtat ttcataactt tcatcaagct tgtatctcat ggctatatcg
     2281 taaagggtat cgccgtcttt ataggctata acatcggcaa cctccccgac agagtatata
     2341 atacgttctc cgggaaaagc aacaagaacc gactgcggtt caaccgttat atcaatatac
     2401 ttagacatat ccttcgcatc tatcctgtac tcaaaagatt ctccacctga atttatgacc
     2461 ataagccttc ctgaaaaatc ttccttgaac cctgccacag aaaatattct tgccaaggta
     2521 agggattcgt caaagtcaaa actgatttta ccggtggatt tgaaagatcc attcagataa
     2581 accgtatttt tcaagggttc tataataatg taagcttttg ctgtaagggt atccgaaaca
     2641 gaaagctcct tctcttcaca cttaatatac tcaactgcct taggattcat cgcaagtttg
     2701 ctcaaaagca tcagtacggt gaactcatcc ttctttttaa aatcaacttt cgtaacgttt
     2761 tgattaatca ttgcaaaaac atacttttcg tatcttttaa agaaaaccgt atcggatttt
     2821 tctattttaa aatcttttcc gtcggctttc aaaccgaata taatatctga aaggctgaat
     2881 ttaagtaggt tatcccccct caggatactt atctcatcat caacttcctc atatgtaact
     2941 ccaagagagt tgaaaacatc ctttaaagtc atcatatccg tatactcaag cactttggtt
     3001 gcaaagtctg aatacacgta aacattgttt ttggaaggag ttataaaaag ataatctcct
     3061 ttcttaagtc caaagtctt
//
LOCUS       DAOSHR010000231         3035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_30542_length_3035_cov_4.691611, whole genome shotgun sequence.
ACCESSION   DAOSHR010000231 DAOSHR010000000
VERSION     DAOSHR010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3035
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30542_length_3035_cov_4.691611"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1008)
                     /gene="secY"
                     /locus_tag="PLU28_09925"
     CDS             complement(<1..1008)
                     /gene="secY"
                     /locus_tag="PLU28_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOG35416.1"
                     /translation="MANNMGNAIKNIFKIPELRDRVIFTILALIAFRIGIFIPIPGIN
                     IESWKGALSGLQSGAAGGFINFFDVFAGGALSNFSIFVLSVNPYINASIILQLLGSII
                     PSLKEMLKEGEQGRKKFGFYTRVLTVVLAVLQGLMMAFTSMNYRAVNSSGEPISAFAY
                     IILATTSIVAGSMFVLWIGEMITEKGIGNGISVLIFAGIVSRYPSYAAQALVALTPFE
                     WILLLVIAVVIVITSIAVQQAERRIKVQYAKRVVGNKVMGGSTTYIPIKVNGGGVIPI
                     IFASVIVSLPSMISSFTPWQWDDSFFKLGGAFYLIVYAILIIFFTYFYSSVVMDPKEV
                     SD"
     gene            complement(1008..1463)
                     /gene="rplO"
                     /locus_tag="PLU28_09930"
     CDS             complement(1008..1463)
                     /gene="rplO"
                     /locus_tag="PLU28_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOG35417.1"
                     /translation="MALRIEDLKPTPGSRTKSIRVGRGPGSGIGKTSGRGMNGQGKRG
                     RGKVRIGFEGGQTTIYRRTPMIGFTNAKFRVTFAEVNLLTLEARFESNEEVTIEKLVE
                     RNILKDLKDGVKILGKGELTKPLVVKANAFSESAKQKIEAAGGKVEVIK"
     gene            complement(1478..1663)
                     /gene="rpmD"
                     /locus_tag="PLU28_09935"
     CDS             complement(1478..1663)
                     /gene="rpmD"
                     /locus_tag="PLU28_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HOG35418.1"
                     /translation="MKSLKIKLVRGRAGKNYNQLATLDALGLKKKDREVVKPDNAQIR
                     GMIRVVEHLVTVEEIDN"
     gene            complement(1666..2199)
                     /gene="rpsE"
                     /locus_tag="PLU28_09940"
     CDS             complement(1666..2199)
                     /gene="rpsE"
                     /locus_tag="PLU28_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOG35419.1"
                     /translation="MSFSKNVKATDVAKEMEEKIIEIRRVAKVTRGGKTMSFRVVAVV
                     GNKNGKVGVGIGNAREVPQAIRKAIQDAKKNVKEIPIKNGTIPHEVIGRHDSSDVILI
                     PAGPGTGVIASTPVRAVVELCGATNILTKSLGSTTVLNLAKATMNGLEQLKSPLMIAQ
                     LRDITVKQIFSGAHKEA"
     gene            complement(2218..2586)
                     /gene="rplR"
                     /locus_tag="PLU28_09945"
     CDS             complement(2218..2586)
                     /gene="rplR"
                     /locus_tag="PLU28_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015302640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOG35420.1"
                     /translation="MIKQLDRKEERAKRHLRIRRTLSGTAERPRLVVFKSGKHIYVQI
                     IDDVQGHTLAAASTSDKELKGKLEKTWTVEASKIVGKLIGERAKAKGISKVAFDRAGY
                     KYHGRIKALADSAREAGLEF"
     gene            complement(2619..>3035)
                     /gene="rplF"
                     /locus_tag="PLU28_09950"
     CDS             complement(2619..>3035)
                     /gene="rplF"
                     /locus_tag="PLU28_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOG35421.1"
                     /translation="EGLIDVTENENTIKRASDKKQITALRGTYAALIRNMIKGVTVGF
                     SKELEIVGIGYRAAMQGEKLVISVGYSNPVEYVAPKGIKLEVPAPNKIVIKGNDKYLV
                     GEVTAIIRKFRQPNSYSGKGIKYVNEVIVKKEGKKV"
BASE COUNT          770 a          710 c          504 g         1051 t
ORIGIN      
        1 atccgaaact tcttttggat ccattacaac tgaactgtag aaataggtga agaagattat
       61 cagtattgcg taaacgataa gataaaatgc tcctccgagt ttaaagaacg aatcatccca
      121 ctgccatgga gtaaatgacg atatcatgct tggaagagat actattaccg atgcaaatat
      181 tatcggaatg actcctccgc cattgacttt tataggaatg taggttgttg atccgcccat
      241 aactttattt cctacaactc tcttggcata ctgtactttt attcttcttt ccgcctgctg
      301 aaccgcaata gatgtaataa ctatcacaac agctattaca agaagaagaa tccattcaaa
      361 aggagtaaga gctaccagtg cctgagcggc atagctggga tacctggaga cgataccggc
      421 aaatataagc actgaaatgc cgtttccgat acccttttca gtaatcattt caccaatcca
      481 cagaacaaac atcgaacctg caactatcga tgttgtggca agtattatgt aagcaaaagc
      541 agaaataggt tctcctgatg aatttacagc tctatagttc atagaggtga aagccatcat
      601 caaaccctga agtactgcta acacaacggt caatactctt gtataaaaac caaacttctt
      661 tctaccttgt tcgccttctt taagcatttc tttaaggctt ggaattattg atcccagcaa
      721 ctgaagtatt attgatgcgt ttatatatgg gttaacgctt aaaacaaaaa tggagaagtt
      781 acttaatgct cctccggcaa atacatcaaa gaaatttatg aaaccccctg ccgcaccact
      841 ctgcaacccg cttaatgcac ctttccaact ttctatgttg attccaggaa ttggtataaa
      901 aataccaatc ctgaaagcaa tcaaagctag tattgtaaat attactcgat ctcttaattc
      961 aggtattttg aagatgtttt taatggcatt gcccatattg ttggccatta ctttatcacc
     1021 tcaaccttac cgcctgctgc ctcaattttt tgcttcgctg attcagagaa tgcattagct
     1081 tttacaacga ggggtttggt gagctctcct ttacctaata tttttactcc gtctttcaga
     1141 tctttaagta tatttctttc tacaagcttt tctattgtta cttcttcatt tgattcaaat
     1201 cttgcttcta aggtaagaag gtttacttcg gcaaacgtta ctctgaactt tgcattggta
     1261 aaaccaatca tcggagttct tctgtaaata gttgtctgtc cgccttcaaa acctattctg
     1321 acttttcctc ttcctctttt accctgaccg ttcatacctc ttccagaagt cttgccaatt
     1381 cctgatccag gacctcggcc aactcttatg ctcttagttc ttgaacctgg tgtgggtttt
     1441 aggtcctcta ttctgagtgc cataattgct taccccctca attatctatt tcctcgactg
     1501 taacgaggtg ttctactacc cttatcatgc cacgaatctg ggcattatcc ggctttacta
     1561 cttctctatc tttctttttg agtccgagtg catcaagtgt cgcaagttgg ttataatttt
     1621 tgccagctct tcctctgact aattttattt ttaagctttt catcattaag cctccttgtg
     1681 agcaccgctg aaaatctgtt ttaccgttat atctctgagc tgagcaatca tgagcggcga
     1741 tttaagctgt tcaagaccgt tcatcgtggc ttttgcaaga ttaagaaccg ttgtagaacc
     1801 caatgatttt gtaagaatgt ttgttgctcc gcaaagctca accacagctc tcacaggtgt
     1861 tgaagctata actccggtac caggaccggc aggaattaat ataacatctg aggagtcgtg
     1921 tcttcctatt acttcgtggg gaattgttcc attttttatc ggaatttcct tgacattctt
     1981 cttggcatcc tgaattgctt ttcttatagc ctggggaact tctcttgcat tacctatgcc
     2041 cactccgact ttaccgttct tgtttccaac aacggcaaca accctgaaag acatggtttt
     2101 tccgcctctt gtaacctttg caactcttct gatttcgatt atcttttctt ccatttcttt
     2161 tgcaacatct gttgccttaa cattcttgga gaatgacatt gctttaagca cctcccgtta
     2221 aaattcaagt ccggcttctc ttgctgaatc tgcgagagcc ttgattcttc catgatactt
     2281 gtagcctgct ctgtcaaagg caactttgct tattcccttg gcctttgctc tttctcctat
     2341 aagctttcca actatcttcg atgcttccac tgtccatgtc ttctccaact ttcccttgag
     2401 ttccttgtcg cttgttgaag cagctgcaag agtatggccc tgaacatcgt ctattatctg
     2461 aacgtatatg tgctttccgc ttttgaaaac gaccagtctt ggtctttcag cagttccgga
     2521 aagagtcctt ctgattctca aatgtctttt agctctttct tcttttctgt cgagctgttt
     2581 tatcataaaa gtctgccctc cttattacta tccagttatt aaactttctt tccttctttc
     2641 tttacgataa cttcattaac atactttatt ccttttccgg agtaagagtt cggctgcctg
     2701 aactttctga tgatagcagt tacttctcct acaaggtatt tgtcattgcc cttaattact
     2761 atcttgttag gtgcgggaac ttcaagcttt attcccttgg gggcaacata ctccacagga
     2821 tttgaatatc ctacggaaat gacaagtttt tcaccctgca tagccgctct gtaacctatc
     2881 ccgactattt caagttcttt ggaaaaacca acggttactc cctttatcat atttctgata
     2941 agtgcagcat aagtacctct taaggctgta atctgtttct tgtctgaagc tcttttaatt
     3001 gtattttcat tttcagtaac atctataaga ccttc
//
LOCUS       DAOSHR010000232         3031 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_30610_length_3031_cov_6.165323, whole genome shotgun sequence.
ACCESSION   DAOSHR010000232 DAOSHR010000000
VERSION     DAOSHR010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3031
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30610_length_3031_cov_6.165323"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..709)
                     /gene="sdaAB"
                     /locus_tag="PLU28_09955"
     CDS             complement(50..709)
                     /gene="sdaAB"
                     /locus_tag="PLU28_09955"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HOG35422.1"
                     /translation="MSIINVIGPVMVGPSSSHTAGAVKIGKFAYNYIGGIPQTVRFIL
                     HGSFATTYMGHGTDKALLSGIIGFNVDDIRIKDIFEIIKKYDLRYEFLFEDLGDVHPN
                     TVRIIAEKDSVEYKIQACSVGAGEILVNEIDGNSVKLNGHTPALSILNKDITGALSEI
                     LKIISAHGINVANVTLNRVSKIFEEASCIIELDEAPSYMLVQELQKSSYVISCRYIPE
                     I"
     gene            823..1938
                     /locus_tag="PLU28_09960"
     CDS             823..1938
                     /locus_tag="PLU28_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35423.1"
                     /translation="MAYIKKYLFILVFLCFSSLIFSFNFLSLKWSFIDTQNENIIVKG
                     TEKDGNYIAFQSISKEQYTNILSFTKEGTVTSKVKISNFNFIDYQILEENLYMLGTYN
                     SAGTNFLSLICMDKDYNVLFRKYYSKGNISPINIRTYGNELIITANSVSSEKSTLYLF
                     TVDKYTNIIQENSYEFSMTLEIKDSVITNDMLYMTGFLDNKESKLDLFVKSINLKTKK
                     FVLEKVWGAKYNEVGTKIIPSEGYLIIVGTNESFNEIKTRDVWVMKISATGDTVWSRI
                     YDSSFIDEVNDACVDEMENIYITGTSITQESQDLFVLKLNKFGEKLYFDIDKDKETNL
                     SSFIFEDSSNIILGGYFINTDTGSDTSVSTGIIMVLN"
     gene            1962..>3031
                     /locus_tag="PLU28_09965"
     CDS             1962..>3031
                     /locus_tag="PLU28_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HOG35424.1"
                     /translation="MKKNIFLVIFLAVSVLIFSSVLTPISQLKDMRDLTTVTIEGIVT
                     VEPGPFENNIFFLQDNTGGVNIYSEGIDISSYGIKRGQSVRVNGYVWTHKMNTEVVVN
                     TSNSNFYIEILSHNQTELLPRLISTNEVNDNKNEGDLIKIKGKITRIVPPEVYVNDGS
                     GEALVYIRENTGIDVSFFKENIDFECIGVLGQYLTKHELWPRSIDDITTSDIVPPVHL
                     LSSLKDEYTVSILFSEPLKQIPAESFRVQSNQIVSISYSFFDRIVTIKTQNPILNQKI
                     FIRGLEDMNNNLVNLYTVYIDSKKDSKENKVLFDESHGQKSGNADWIAEGGYSDFADA
                     LKAEGFTIFSLKEDFSSDILNM"
BASE COUNT         1042 a          531 c          489 g          969 t
ORIGIN      
        1 atataagcac agaaaaacac agcacaagcc ttttcataac acgcctcctt tatatttctg
       61 gaatatatct gcaggaaatt acataagaag acttctgaag ttcttgaaca agcatatatg
      121 atggagcctc atccagttca attatacagc tggcttcctc gaatatttta cttactctgt
      181 tcaaagtaac gtttgcaaca tttattccat gagcagatat tattttgaga atttctgaaa
      241 gtgcacctgt tatatctttg tttagtattg acaaggcggg agtatgtccg tttagtttta
      301 cgctgtttcc atcaatctca ttgacaagga tctcccccgc tcctactgag catgcctgta
      361 ttttatactc gacagagtcc ttttctgcta taattcttac tgtattaggg tgaacatctc
      421 ccagatcctc aaaaagaaac tcatatctta aatcatattt tttaattatt tcaaatatat
      481 cttttattct tatatcatca acattaaatc ctattatgcc gctaagaaga gccttatctg
      541 ttccgtgtcc catgtatgtg gttgcaaaag aaccgtgaag tataaacctt actgtctgtg
      601 gaatacctcc gatatagtta tatgcaaact tacctatctt tactgctcca gccgtatgcg
      661 agctggaggg accaaccatg accggaccga ttacatttat tatggacatt tttctcctcc
      721 aaaaacgaat tttttaaaat gttttagtaa cataattcaa ttatagctta ttttgttgta
      781 taataaaata tgataacata aaatatcggg aggatacggt taatggctta tataaaaaaa
      841 tacctcttta tcttggtttt tctttgcttc tcgtcattaa ttttttcatt taattttctt
      901 tccttaaaat ggtccttcat agatacacaa aatgaaaata taatcgtcaa gggaacagaa
      961 aaagacggca actatattgc atttcaaagc ataagtaagg aacagtatac taatatactc
     1021 tcattcacca aagaaggcac tgtgacttcc aaagttaaaa tctccaattt taattttata
     1081 gattatcaga tacttgaaga aaatctctac atgctgggta catataattc tgccggtacc
     1141 aacttccttt ccctgatatg catggataaa gattataacg tacttttccg aaaatactac
     1201 agcaaaggaa atatttcacc cattaatatt cggacttacg gcaatgagct tataataaca
     1261 gctaactctg tttcaagtga aaaaagtaca ttatatcttt tcacagttga taagtacaca
     1321 aatataattc aggagaacag ctatgaattc tcaatgactc ttgaaataaa agattctgta
     1381 ataacaaatg acatgttgta tatgacagga tttcttgata acaaagaatc taagctggat
     1441 ctttttgtaa aaagcataaa cctgaaaacc aaaaaatttg ttcttgaaaa ggtttggggg
     1501 gctaaataca atgaagtcgg aacaaaaata ataccttcag aaggatatct tattatagtt
     1561 ggaacgaacg aatctttcaa cgaaataaaa accagagatg tttgggtaat gaaaatttcc
     1621 gcaaccggag ataccgtatg gtcgagaata tatgacagtt catttattga cgaagtaaac
     1681 gacgcatgtg ttgatgaaat ggaaaatatt tatataaccg gaacatctat aacccaggaa
     1741 agtcaggatc ttttcgtctt aaaacttaat aaattcggcg aaaaacttta ttttgacatc
     1801 gataaggata aagaaaccaa tctttccagt tttatatttg aagattcttc aaatataatc
     1861 ttgggagggt atttcataaa caccgatacc ggcagcgata cttctgtttc aaccggaatt
     1921 attatggtac taaattaata aagagaaagg acaaaataat tatgaaaaag aatatttttt
     1981 tagtgatttt cttagcagtt tctgtattaa ttttttcttc tgtacttact cctataagcc
     2041 agcttaaaga tatgagagat ctaacaacag ttacaataga aggtatagtc actgtcgagc
     2101 caggaccttt tgaaaacaat attttctttt tgcaggataa caccggtggt gtaaatattt
     2161 actccgaagg cattgacata agcagctatg gaataaaacg cggtcagagc gtacgagtca
     2221 acggatatgt ttggacccac aagatgaata ccgaagttgt tgtaaatact tcaaattcaa
     2281 acttttatat tgaaatactt tctcataatc aaacagaact tcttcctaga cttatctcaa
     2341 caaacgaagt aaatgataac aaaaatgagg gtgatttgat aaagataaaa ggaaagatca
     2401 ccaggatagt tcctccagaa gtttatgtta atgacggttc cggtgaagct ttagtctaca
     2461 tccgcgaaaa cacagggatt gatgtttctt ttttcaaaga gaatattgat tttgagtgta
     2521 tcggagtttt aggtcagtat cttaccaaac acgagctttg gccaaggagt attgatgata
     2581 tcaccaccag tgatattgtt cctccggttc atctgctttc ttccctcaaa gatgagtaca
     2641 cagtaagcat tcttttttct gaaccactaa aacaaattcc tgccgaatct ttcagagtac
     2701 agtccaatca aattgtatca atttcatact ctttcttcga taggattgtt acaataaaaa
     2761 ctcaaaatcc aatccttaat cagaaaatat tcatacgtgg acttgaagat atgaataata
     2821 atcttgtcaa cctttacaca gtatatattg attcaaaaaa agattctaaa gaaaacaaag
     2881 ttcttttcga tgaatctcac ggacagaaat caggaaatgc cgattggata gccgaaggag
     2941 gatactccga ttttgccgat gccctcaaag ctgaagggtt tactatattt tctctaaaag
     3001 aggatttttc atcagacata cttaatatga t
//
LOCUS       DAOSHR010000233         3015 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_30845_length_3015_cov_4.335135, whole genome shotgun sequence.
ACCESSION   DAOSHR010000233 DAOSHR010000000
VERSION     DAOSHR010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30845_length_3015_cov_4.335135"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(87..2150)
                     /locus_tag="PLU28_09970"
     CDS             complement(87..2150)
                     /locus_tag="PLU28_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35425.1"
                     /translation="MKKLFFVSFLLLICISAFPFSVLNEEPFFSKTSVLSFLKDVFGY
                     TDSKRITLNIQKAYDSDANVWNIYSDFYSFSAEIDADTGTLRRYYFNGTTKRFLPSFT
                     RKQAYEKAIVFLKKIGLYDQQKFNMEYTLPENSSYSKNQVHLYYDFAFPRIIESIRFL
                     PDFSQDGITISIDPSDGLIQSFNYNWSDFDDSMIHTKKITSLQAEKEFQKKIMVLPTV
                     KSFWNDDEGVTKYILCYDFPTFNSNIPKIDSINGMLIDEYGDEINLLYNSDSTVYSVK
                     NEVHFESTALKTPMTKEEALHFTKNFFNSTLNVFMNVDYEVNYSGLPDERIWNISIYD
                     QHSSHPANANISIDAIYGKINNLYYSSFPLNGEAPLKTSSQALERSEEILKSLSVQND
                     FKPFYISSDDKSYVIDFKRILKNGVIFGPDSLIIRLDKYTLQCLYINLSYSSKFDIEA
                     SEALISQEEAFSILSAERNFGLYLTKSPSYNYWGEYIQPKTFAVYTVIPYSEIYLKTD
                     GKLINPNGYVVKETIWSSNNKLPESISHTAQILYDNNLIDYEFIKTLNNSITIHDALK
                     VLSAVTNSKNMKLAGINSEYNIFNDPLYAKELNMSQDTILTKELMSGIIGKFLNLKPY
                     VNCPEIFGTIFTDQEEISQEYLPYSMLLYASKIIPVTQNTYKPKQPITGHELITAIFR
                     ALELM"
     gene            complement(2162..2932)
                     /locus_tag="PLU28_09975"
     CDS             complement(2162..2932)
                     /locus_tag="PLU28_09975"
                     /inference="COORDINATES: protein motif:HMM:NF018387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 family protein"
                     /protein_id="HOG35426.1"
                     /translation="MNLFKTISLLSIILVTLILIFFIFTLQIKINQNERFYRLDLNGN
                     GFPDVAELNYDDSENFRNWFTSIILNVVVNRSTVLPENYSDCAGLVRYAYKEALKKHD
                     PIWIEQSGYRGIVFEDIQNYNYPNVPALKQLLFKINNNKYYYDEVPSDFSVFASARHM
                     LEYNTVFVSKDIYAAKSGDVIGFFHPEDPEFPYHIMVYLRNNTESYILYHTGPVDERG
                     GELRIVRLEDMFFADPSWLPSYQNKYFMGVFRFKILSY"
BASE COUNT          975 a          471 c          523 g         1046 t
ORIGIN      
        1 ttaattatat tttctataaa aaatcacaat ggtataagaa gagagttcat taaaaatccc
       61 ggcattttat ctatgccggg attttttcac attaattcaa gagctctgaa gattgctgtt
      121 ataagttcat gacctgttat cggttgcttg ggtttgtatg tgttttgtgt aacgggaatt
      181 atctttgatg catataataa catgctgtag ggtaaatact cctgagatat ttcttcttgg
      241 tcagtgaaga tcgtaccgaa tatttccgga cagttgacat aaggctttaa gtttagaaat
      301 ttacctatta ttccggacat taattctttt gtgagaatag tatcctgaga catgtttaat
      361 tctttcgcgt ataaaggatc gttaaatatg ttgtattctg agtttatacc agccagtttc
      421 atgttttttg agttagttac tgctgaaaga actttaagtg catcgtgaat ggttatcgaa
      481 ttattaagag tctttatgaa ttcatagtcg attaagttat tatcgtataa aatctgagct
      541 gtatgactta tgctttcagg aagcttattg ttggaactcc atatagtttc ttttactaca
      601 taaccatttg gatttataag tttaccatct gttttaaggt atatctcgga ataaggaata
      661 actgtatata ctgcaaaagt ttttggttgt atatactctc cccagtaatt gtaactagga
      721 cttttggtaa gataaaggcc aaagtttctt tcggcagaaa gtattgaaaa agcttcttcc
      781 tgagatataa gcgcttcaga tgcttcaata tcaaactttg agctatagga taagtttata
      841 taaagacatt gaagggtgta cttatctaat ctaataataa ggctatcagg accgaagatt
      901 acgccatttt tcaaaattct tttgaaatct attacataac ttttatcgtc ggatgatata
      961 taaaaaggct taaaatcatt ctgtaccgat aatgatttaa gaatttcttc tgatctttca
     1021 agtgcttggc ttgatgtctt taagggagct tctccgttta aaggaaaaga gctgtagtat
     1081 agattattaa tttttccata tattgcatca atacttatgt ttgcatttgc aggatgtgat
     1141 gagtgctgat catagatact tatattccaa atgcgctcat cgggaagacc cgagtaattt
     1201 acttcataat ccacgttcat aaatacattt agagtactat tgaaaaagtt ttttgtgaag
     1261 tgaagtgctt cttccttggt cataggcgtt ttaagagctg tactttcaaa atgaacttca
     1321 ttttttacag aataaacagt tgaatcacta ttataaagca gatttatttc atctccgtac
     1381 tcatcaatca acataccgtt tattgaatct attttaggaa tgttagagtt gaaagttggg
     1441 aaatcataac ataaaatgta tttggttact ccttcatcgt cattccaaaa ggatttcaca
     1501 gtaggaagta ccataatttt tttctgaaat tctttttctg cctgtaaaga ggtaattttt
     1561 tttgtgtgaa tcattgagtc atcaaaatct gaccaattat agttaaagct ttgtatcaga
     1621 ccgtcagaag gatcaatcga tatagttata ccatcctggc tgaaatcagg aagaaatcta
     1681 atactttcta ttatacgcgg aaatgcaaaa tcatagtaaa gatgcacttg attttttgaa
     1741 tatgaactgt tctccgggag agtatactcc atgttaaact tctgttgatc atagagtcct
     1801 atctttttta aaaatactat tgctttttca taagcttgct tacgtgtaaa tgaaggtaaa
     1861 aaccttttgg tagtgccgtt gaaatagtac cgtctgagag taccggtatc ggcatcaatc
     1921 tcggcactaa atgaatagaa atccgagtaa atattccata cgtttgcatc cgaatcataa
     1981 gccttttgaa tattcagagt tattcttttg gagtcagtat agccaaagac atcctttaaa
     2041 aaagatagca cggaagtctt tgagaaaaaa ggttcttcgt taagaacgga aaaagggaaa
     2101 gcagagatac agatcaatag taaaaaagag acaaaaaaaa gttttttcat gattcacctc
     2161 ttcaatatga aagaatttta aatctgaaaa cacccataaa gtatttattt tgatatgacg
     2221 gtagccagga agggtcggcg aaaaacatat cttcaagtct tacgattcta agttctcccc
     2281 ctctctcatc tactggacct gtgtggtaaa gaatatagct ttcggtatta ttccgcaagt
     2341 ataccattat atgataagga aattctggat cttcagggtg gaaaaagcct ataacatcac
     2401 cggattttgc tgcatatata tccttggaaa caaaaaccgt attatattcc agcatatgtc
     2461 gtgcagaagc aaaaactgaa aaatctgatg gaacttcgtc ataataatac ttattattat
     2521 taattttgaa gagcagttgt tttaaagcag gtacattagg ataattataa ttctgtatat
     2581 cttcaaagac tatgcccctg tagccactct gttctatcca gattggatca tgctttttca
     2641 aggcttcttt atatgcgtat ctgacaagcc cagcacagtc agagtaattt tctggaagaa
     2701 ctgtactgcg gtttacaacc acattaagta taattgatgt gaaccaattt ctgaagttct
     2761 cagaatcatc ataattaagt tcggcaacat cagggaaacc gtttccgtta agatcaagcc
     2821 tgtaaaagcg ttcattctgg ttaattttta tctgcagtgt gaatatgaaa aaaataagta
     2881 taagagtaac taaaataatt gaaagtaacg aaatagtttt aaataaattc atattttttt
     2941 ccttctgtca ttaagaaatt tgtgtatatt cccttcccag acattcataa aagtcagata
     3001 gtactcttta tactc
//
LOCUS       DAOSHR010000234         3008 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_30949_length_3008_cov_4.359296, whole genome shotgun sequence.
ACCESSION   DAOSHR010000234 DAOSHR010000000
VERSION     DAOSHR010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30949_length_3008_cov_4.359296"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..672)
                     /locus_tag="PLU28_09980"
     CDS             complement(<1..672)
                     /locus_tag="PLU28_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35427.1"
                     /translation="MKKMWLTVFILCLFGLVFSAVTLEEPYYKKYKYWTSNGYADAAG
                     VFIAPPLNASVLDKGQSIEFEVCVPYSGLYNIQLVYTVLSDELAENEAQVKINGEYQY
                     YESRRVILPQYWKSQSTGFAVDRYGNQLVPLQTKYKGRIVYTLRDSAFYKPEPLRFFL
                     KEGINNINLAIESGKILLEGLALKSYTPLLPYESYISKYGYLKNENNFFLSIEAESID
                     YKNDTS"
     gene            complement(686..2002)
                     /locus_tag="PLU28_09985"
     CDS             complement(686..2002)
                     /locus_tag="PLU28_09985"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOG35428.1"
                     /translation="MLKKVLPVLLVFMVFAGVLVGKTVITYANWNLGLELETNIVKEF
                     MKAYPDIEVRFAENIDYGKYIDSLNAAAASGKLPDVIMIPNIPMALTNEWALNIKDMV
                     RKDIEWMNVPTPLRAAASFEDGVYAVPAGMYFMGYFVNDDLFKKYNVSSLSFSPTWQS
                     FMNAVRTLSNPAEKILGLCEEVQIPEWYPASLNADLGWFTWDGSKYNLDGKEFITAVK
                     LAKQLYTNKYVFDSLTEQEKKLFNAGWYGEAWDQGEIAVRWSGSWDVNSFKNLKFNSR
                     FIGVPGGRTPVVGDFMVISSSTKNAEAAYKFIRYITFAREGMLKRLEIDEKKQWSSLP
                     LTTEKAIINKYFEKNAAFPGIKEAFATIDSGIIEGVKIVPGYIESRWTSPTGVKIGSN
                     DNANIGDVIWNAMRGNVNIADYASALNTLAGKAHQSGYNTIISIIK"
     gene            complement(2031..>3008)
                     /locus_tag="PLU28_09990"
     CDS             complement(2031..>3008)
                     /locus_tag="PLU28_09990"
                     /inference="COORDINATES: protein motif:HMM:NF028476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 36"
                     /protein_id="HOG35429.1"
                     /translation="RGVPLMGDNDWNDGLSAVGTDWKGESFWMSEFFYMVLGQFMDLG
                     SYAKDSVFLKKVEDVMQTLKESFEKYGWDGKWYLQATTDGFEKIGSNENEEGKIFLNP
                     QLWSLISSIGDSQRAVTAMENVTEYLLRDYGALLLAPAYTAPRTDIGYITRYAPGLRE
                     NGGVYTHAATWAVWAYSLLKEGNLAYEAYKRICPPNRCSDIDLYSAEPYVTAGNSDGP
                     LSPYFGRGGWSWYTGSAQWLERVALNHILGIQSRTDGLLVEPCLPEGWDGFSCTRKFR
                     NACYIIEVSNPKKVSTGIKEVYIDGAVYGSNLLPVFNDGKDHHVRVVMG"
BASE COUNT          877 a          722 c          510 g          899 t
ORIGIN      
        1 agaagtatcg tttttataat ctatactttc tgcttctatt gaaaggaaaa aattattttc
       61 attttttaag tacccgtatt ttgaaatata agattcataa ggtaaaagag gagtataaga
      121 ctttaaagcc aaaccctcaa gaagaatctt tccgctttca atagcaaggt ttatattatt
      181 aataccctct ttcagaaaga atcttaaagg ttccggcttg taaaaggcag agtcacgcag
      241 agtataaact atccttccct tatactttgt ctgaagcggt acaagctggt ttccatacct
      301 gtcaactgca aaccctgttg actgtgattt ccaatattga ggcagaataa cccgtcttga
      361 ttcataatac tgatactcac cgtttatttt cacctgggct tcattttcag caagctcatc
      421 tgaaagaacg gtgtacacaa gctgaatatt ataaaggcca ctgtacggaa cacaaacttc
      481 aaactcaata ctctgacctt tatcaagaac tgatgcattt aagggcgggg cgataaaaac
      541 ccccgccgca tctgcatacc cattggaagt ccagtattta tattttttat aatacggctc
      601 ctccagcgta actgctgaaa aaaccaaacc gaataaacaa agaataaaca ccgtcagcca
      661 catctttttc aaagtaacct tctccctact ttattatgga tattatagta ttatatccgc
      721 tttggtgagc cttgccggca agagtgttca gagccgatgc gtagtccgct atgtttacat
      781 tacctctcat ggcattccat attacgtctc ctatgttggc gttatcgttt gatccgatct
      841 taacacctgt tggcgatgtc catcttgact caatataacc gggaacgatc tttacacctt
      901 cgattatacc tgagtctatg gttgcgaaag cctcttttat gcccggaaat gcagcgtttt
      961 tttcaaaata cttgtttatt atggcttttt ccgtggtaag cggcagtgat gaccactgtt
     1021 tcttttcatc aatttcaagt cttttcagca tgccttcacg cgcaaaggtt atatatctta
     1081 taaacttata ggctgcctct gcatttttgg tagatgaaga gataaccatg aaatctccga
     1141 ctacaggtgt tcttccgcct ggcacaccta taaaccttga attaaattta aggtttttga
     1201 agctgtttac atcccatgag cctgaccatc tcacggctat ttcaccctga tcccatgctt
     1261 ctccatacca tccggcatta aaaagctttt tctcctgttc agtaagactg tcaaaaacat
     1321 acttgttggt ataaagctgt ttggcaagtt ttacagccgt tataaattcc tttccgtcaa
     1381 gattgtactt tgaaccgtcc catgtgaacc atccaagatc agcgttaaga gaggcaggat
     1441 accattctgg aatctgcact tcttcacaaa gtcccagtat cttttcggca ggattagaga
     1501 gtgtgcgtac agcattcata aaactctgcc atgtaggtga gaatgaaagt gaagaaacat
     1561 tgtacttctt aaaaagatca tcgtttacaa agtatcccat gaagtacata cccgccggaa
     1621 ccgcatatac tccgtcctca aaagaagcgg cagcacgtaa aggcgtcggt acattcatcc
     1681 actcaatatc tttccttacc atatctttta tattcagtgc ccattcgttg gtaagagcca
     1741 taggaatatt cggtatcatt atgacatccg gaagcttgcc ggaagctgca gcggcattaa
     1801 gagaatcaat atactttccg taatcaatgt tttctgcaaa tcttacctca atatccggat
     1861 acgctttcat aaactccttg actatgttgg tttcaagttc aaggccaagg ttccagttag
     1921 cataagtaat gactgtctta cctacaagca cgcctgcaaa taccataaat actaataata
     1981 ccggtaatac cttttttaac atacatatcc ctccctgtaa aattatgata ctatcccatt
     2041 actaccctta catgatgatc ttttccatcg ttaaaaaccg gaagaagatt agaaccatag
     2101 acggctccgt ctatataaac ttctttaatc ccggtggata ccttcttagg attagatacc
     2161 tctataatat agcaagcatt tctgaacttt cttgtacagc tgaaaccatc ccatccttcc
     2221 ggcaaacatg gttccacaag caacccgtct gttctggact gaatacccag aatatggttt
     2281 aaagcaaccc tttcaagcca ctgagccgag ccagtatacc aactccaccc gcctctgccg
     2341 aaatacggag aaagaggacc atcggaattt ccagcggtaa cataaggctc tgcagagtaa
     2401 aggtctatat cgctgcaacg gttcggcgga cagattctct tataagcttc atatgcaaga
     2461 ttaccctctt tcagtaaaga gtaagcccat actgcccatg ttgctgcatg agtatagact
     2521 ccaccgtttt ccctaagccc tggtgcatat cttgtaatat atcctatatc ggttctagga
     2581 gcagtatatg caggagccag aagtaaggcg ccataatccc taagcagata ttccgtaaca
     2641 ttttccatag ccgttacagc tctctgactg tctcctattg agcttataag cgaccatagc
     2701 tgtggattaa gaaatatctt cccttcttca ttctcattag acccgatctt ctcaaaaccg
     2761 tccgtagtag cctgcaaata ccattttccg tcccacccgt acttttcaaa agattccttt
     2821 agagtctgca taacatcttc cacttttttt aaaaagacac tgtccttggc ataagaaccc
     2881 aaatccataa actgcccaag aaccatatag aaaaattctg acatccaaaa actttcacct
     2941 ttccagtcag tgccaactgc gctcaggcca tcattccagt cattatcgcc cataagcggc
     3001 acacctcg
//
LOCUS       DAOSHR010000235         2993 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_31174_length_2993_cov_5.684139, whole genome shotgun sequence.
ACCESSION   DAOSHR010000235 DAOSHR010000000
VERSION     DAOSHR010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31174_length_2993_cov_5.684139"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..138)
                     /locus_tag="PLU28_09995"
     tRNA            complement(48..138)
                     /locus_tag="PLU28_09995"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(101..103),aa:Ser,seq:cga)
     gene            complement(182..766)
                     /locus_tag="PLU28_10000"
     CDS             complement(182..766)
                     /locus_tag="PLU28_10000"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOG35430.1"
                     /translation="MIKKIFFSCVTLLIACLIIFLAQKALTKISRGEKLDPMQVYYIC
                     QKNKPQTGNIPEELIFSIAMTESSGYIKARTEYAYGLTGLSRIALREINAEFKMNIQY
                     SDLENPETAVIASYAYMQSLYNRWKNINQSMDDYTVLQLSVISYVWGIGNTLKWLNNT
                     NTSNRYIDEAIPQDAKDYLYNISFWSFYGKKNFD"
     gene            complement(841..2388)
                     /locus_tag="PLU28_10005"
     CDS             complement(841..2388)
                     /locus_tag="PLU28_10005"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOG35431.1"
                     /translation="MKKTLSLLFFLVLLTVSVLTAATKFTDVPINHWAYDAVQRITSI
                     KILEGYPDNTFKGVSTVTRYELTLTVDRTISYIQEALLNPLTEKINELDKKIKNIPTT
                     TSTPSKDYSGDIAVLKSDLSELTKRFDNMQTSLTDIRDLKNSYEILGYTVTKIKEVDT
                     RLTTLESKVSGLELSNANSEELSNTKKTVDSILVQYSEMNNSIKSATNKITALETKVA
                     ALEKNSVTESFSEKLAMYDSKITLLETKTATIETKILNNDAKMTSIDTKMAGYDTKIS
                     SIDSKVSGHDTKISSHDSKLSTIEKIIAAHETKLSSYDSKFTANDSKIIALEGGLNAL
                     DARMSAIEKNTLTEDMSKVKDLLTNTNTKLDAKADKITVNAVESRLNTLEQSFQVFAV
                     NGSETSKSIAAIKNDIAALTGKYDEMNKRVMDNTAATDSNTKNIESLTAKTNENSAKT
                     DSLTAKLDESTLKLDQKIDSKVAELDSKITGVDTKWPGINFLDILLSVGLSVGIVYAL
                     NYFGVLK"
     gene            2560..>2993
                     /locus_tag="PLU28_10010"
     CDS             2560..>2993
                     /locus_tag="PLU28_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HOG35432.1"
                     /translation="MLKRTHTCGELSSQDIGKEIVLNGWVDRVRDLGGMLFVLVRDRY
                     GKTQVYFDPAADKQLYESALKLGNEYTVGIKGIVNKRPEKDVNTNMSTGEIEVMAKEL
                     VVFSESETPPIYVNKEDDANENLRLKYRYLDLRKEKLQKNLML"
BASE COUNT          815 a          535 c          650 g          993 t
ORIGIN      
        1 gtgcaaaata aaaacgccag cattgtatca atactgacgt tcttatatgg cggaggcggt
       61 gggattcgaa cccacacgag gctataaacc tcaccggttt tcgagaccgg ccccttagcc
      121 agttcggaac acgcctccaa caatgcataa ttatatcaca aaaaaaatca taaatctaga
      181 gtcagtcaaa atttttcttt ccgtaaaaag accagaagct gatgttgtaa aggtagtctt
      241 tggcatcctg tggaatggcc tcatcgatat aacggttaga ggtgttggtg ttattaagcc
      301 atttgagggt gttcccaatt ccccacacat acgaaatcac gcttaactgc aggacggtat
      361 aatcgtccat actctggttg atatttttcc atctgttata gagcgactgc atatatgcat
      421 acgaagctat aacagccgtt tctggatttt caagatcact gtactgtata ttcattttga
      481 actctgcatt tatttcccgc aaggctatcc tgctcagacc tgtgagacca taagcgtatt
      541 cggtacgtgc tttaatatat cctgagcttt cggtcatggc gattgaaaag ataagttctt
      601 caggaatgtt tcctgtttgc ggcttgtttt tctggcagat atagtacacc tgcataggat
      661 ccagtttctc cccgcgggat attttggtta gtgccttctg agctagaaat attatcaggc
      721 aggctatcag aagtgttaca caggaaaaga aaatcttttt tatcatataa cgccccgcac
      781 gtcttaaaaa taaaccaatg ccttatggca taaagccaga aggcattggt cagtcattga
      841 ttatttaagt actccaaagt agtttaaagc atatactata ccgacagata gtccgacaga
      901 cagaagaatg tcaaggaagt ttattcctgg ccatttggta tctactccgg ttattttgga
      961 atcgagttct gcaaccttgc tgtctatttt ctgatcaagt ttcagagttg attcatcgag
     1021 cttggctgtg agagaatcag ttttggcact gttttcgttt gtttttgctg taagggattc
     1081 gatgtttttg gtatttgaat ctgtagctgc tgtattatcc attactcttt tgttcatttc
     1141 gtcatacttt ccggtaagag cagcaatgtc gttctttata gcagctatgg acttgcttgt
     1201 ttccgaaccg tttactgcaa atacctggaa agactgttca agcgtattga gtcttgactc
     1261 gacggcgtta actgttattt tgtcggcttt agcgtcaagc ttggtattag tattggtgag
     1321 tagatccttg acttttgaca tatcttcggt aagggtattt ttttcaattg cggacattct
     1381 tgcatcaagt gcgttcaaac cgccttccaa tgcaatgatt ttggaatcat tagcagtaaa
     1441 cttggagtcg tatgaactca acttggtttc atgtgcggcg attatctttt caatggttga
     1501 gagcttggag tcatgggacg atattttggt atcatgtcct gagactttgg aatcaattga
     1561 agatatcttg gtatcgtatc cagccatttt ggtgtctatt gaagtcatct tggcatcgtt
     1621 attgagaatt ttggtttcga tagttgccgt cttggtttcc agtagggtaa ttttactatc
     1681 atacattgca agcttttctg aaaaactttc tgttacgcta tttttttcaa gcgctgcaac
     1741 tttggtttca agagctgtta tcttgttggt tgccgattta atggagttgt tcatttccga
     1801 gtactgtacc agaatgctgt cgacagtttt ctttgtgttt gagagttctt cggagttggc
     1861 attgcttaat tcaagtccgg atacttttga ttcaagggta gtaagtcttg tgtcaacttc
     1921 ctttattttg gttaccgtat atccgagtat ctcataggag ttcttaagat cacggatgtc
     1981 agtgagactc gtctgcatat tgtcaaatct tttggtcagt tctgaaagat cggattttaa
     2041 aacagcaatg tctcccgaat aatccttgga aggtgtggaa gtagttgtgg gaatattttt
     2101 aatttttttg tctagctcat tgattttttc ggttaatggg ttcaggagag cctcctgaat
     2161 gtaactgatg gttctgtcta cggtaagggt gagctcgtat ctggttactg ttgatactcc
     2221 tttaaaagtg ttgtctggat atccttcgag tatctttatc gaggtaatcc tctgtactgc
     2281 gtcatatgcc cagtggttta tgggcacatc ggtaaacttt gtggctgccg taagcactga
     2341 aactgtcaaa agcaccagga aaaacaaaag tgataaggtt tttttcacta tgaacacctc
     2401 catgattttc ataagtttga tttttaattt agttataatc taacttctat tttatcataa
     2461 ataatggtaa aatatataga gtgaataaaa gttctacagg atttttaaca ctaggacaca
     2521 catttaattt tttaactctt caaatacgga ggtgcggttt tgcttaaaag aacacacaca
     2581 tgcggagaat tgtcttcaca ggatatagga aaggagatag ttctcaacgg ctgggtagac
     2641 agagtaagag atcttggggg aatgcttttt gtacttgtga gggataggta tggcaaaacc
     2701 caggtttatt ttgatcccgc cgcagataag cagctttatg aaagtgcact caagcttggc
     2761 aatgaatata ctgtaggaat aaagggtata gtcaacaaaa gacccgagaa agatgtaaac
     2821 acaaatatga gcacagggga aatagaggtc atggctaagg aactggtggt attttcagag
     2881 tcagagactc ccccaatata tgtaaacaaa gaagacgatg ccaatgaaaa tcttaggtta
     2941 aagtacagat atcttgacct gcgaaaggaa aagcttcaga agaatcttat gct
//
LOCUS       DAOSHR010000236         2975 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_31435_length_2975_cov_5.376370, whole genome shotgun sequence.
ACCESSION   DAOSHR010000236 DAOSHR010000000
VERSION     DAOSHR010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 2975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2975
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31435_length_2975_cov_5.376370"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1309)
                     /locus_tag="PLU28_10015"
     CDS             complement(<1..1309)
                     /locus_tag="PLU28_10015"
                     /inference="COORDINATES: protein motif:HMM:NF021033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM1802 family CRISPR-associated protein"
                     /protein_id="HOG35433.1"
                     /translation="MQEAVYEIGKIVKSESSIIEGIMLNNKKGEPKRVLKMDFSTQKT
                     ELTVDVSEEIDADSAERYFHIGSASGPNSPQWFSVSKVVDYHLTETLCNLTEVFDDSS
                     TELGKKLKDIFENYTFDMGADVPKKYRYPIDLQKVGIIKESMSDIKKLASNGDGKVEG
                     KKLRSILSGEFLNYLKEEKGLKKDDIGLCVITIDQKPISEYPEYIDKATAEKTGRKEK
                     SEDEGKKQNNGSGYCYFCSEKKDVSSDLKKLDIKYFVNDKISFASFFTEKGFDKNILV
                     CQECFESLMAGEKQLKERLKTKIGYFDVYVVPQFILSTNISKEEFEKISDKIIHTLKV
                     SQNLDSLDKVQGRLQDYILFEDDTNDFYINFVFFEKSNSATKIMKLIKDVKPSRLAKI
                     FEKKNEIMKIFIEYFPENLVKGFNLNYVYFFSVQKIAGKNSYSD"
     gene            complement(1311..1943)
                     /gene="cas6"
                     /locus_tag="PLU28_10020"
     CDS             complement(1311..1943)
                     /gene="cas6"
                     /locus_tag="PLU28_10020"
                     /inference="COORDINATES: protein motif:HMM:TIGR01877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HOG35434.1"
                     /translation="MHDNGFSYEKRQYKMFVFSRLYGNYEMNIKDRSITFKEFCDLYI
                     SSIDDAFIKYFLDGVFEKEILNISGQNITIDKITAVDCVPKKEIKVKTLSPIVVYSTL
                     RTEDGRKKTYYYSPKEEEFEAQIKNNLIKKYIAFYNSEPTDSSFRMDISDRGALKERV
                     ITYKSIILKGWDGVFKVNASEEMMEMMFCTGLGAKNSIGMGFVTPLTKEV"
     gene            complement(2211..>2975)
                     /locus_tag="PLU28_10025"
     CDS             complement(2211..>2975)
                     /locus_tag="PLU28_10025"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOG35435.1"
                     /translation="KGLTGETVKACLKRLRLEKAAYEILRTDKTITEIAFESGYETLE
                     AFTKAFKKAHGISPSRIRKLQDWKGLLYSQAGIHYSPGQRQPYYFFIKPRGGNEMEIK
                     IVNLQPMKLLGLRGTGDYWNMPALWKKFHEILRANKLFSTEAQFLTVFHDHTDDIPQE
                     QKRYDVSISVHKEVSDIDPLSIYQFEGGLYAVLVHVGSCEEIGPVWDKWRSEWLGSSG
                     WKLDKKRPSLEWYQNNPSAVPPELAVTFLCDPVIKE"
BASE COUNT          792 a          596 c          482 g         1105 t
ORIGIN      
        1 aatccgagta actgttcttg cctgctatct tttgcactga gaaaaagtat acatagttta
       61 aattaaaccc ttttaccaaa ttttcgggaa agtattcaat aaaaattttc atgatttcgt
      121 ttttcttttc aaatatcttc gcaagtctgc ttggttttac atcctttatg agtttcatta
      181 tcttagtagc actattgctt ttttcaaaga aaacaaagtt gatgtaaaaa tcatttgtat
      241 cgtcttcaaa aagaatataa tcttgaagcc gtccctgtac tttatccaaa gaatcaagat
      301 tttgagatac ttttagagta tgaataattt tgtctgagat tttttcaaac tcttccttac
      361 ttatattagt actcagtata aactgcggaa caacataaac atcaaagtac ccgatcttgg
      421 tcttaagtct ctctttgagt tgcttctcac cagccataag actttcaaag cattcctgac
      481 agacaaggat atttttatca aatccttttt ctgtaaaaaa acttgcaaaa ctgatcttat
      541 cgttcacaaa gtactttata tccagttttt taaggtctga agatacatct tttttttcac
      601 tgcaaaagta gcagtatcca gaaccgttat tttgtttttt tccttcatct tcagactttt
      661 cttttctgcc ggtcttttct gccgtggctt tatcaatgta ttcaggatac tctgagatcg
      721 gtttttggtc tatggttatg acacatagcc ctatatcgtc tttttttaaa cctttttctt
      781 ctttcaaata atttaaaaac tcaccgctga gaatgctcct aagctttttt ccttctactt
      841 ttccatcgcc atttgaagcc agctttttta tatctgacat agattctttt atgattccaa
      901 ctttttgaag atctatagga tatctatact ttttcggaac atctgcaccc atgtcaaatg
      961 tgtagttttc aaagatatct ttgagctttt ttccaagctc agtactgctg tcatcaaaca
     1021 cctctgtaag attgcacaga gtttcggtaa ggtgataatc aacaacttta gaaaccgaaa
     1081 accactgcgg cgaatttggg ccgctggcac ttccgatatg aaagtacctt tccgccgaat
     1141 cagcatctat ctcctcacta acgtcgacgg taagttctgt tttttgggtg ctaaagtcca
     1201 tctttaaaac tctttttggc tctcctttct tattattgag cataatacct tcaataatag
     1261 agctctcact ctttacaatc tttcctattt catataccgc ttcctgcatc ttatacctcc
     1321 tttgtcagcg gagtaacaaa gcccattccg atggagttct tggctcctaa ccctgtgcag
     1381 aacatcattt ccatcatttc ttcgctggcg ttgactttga aaacaccgtc ccatccttta
     1441 agaattatgg acttgtatgt aattactctt tctttaagag caccacggtc agagatatcc
     1501 atcctaaagc tgctgtcggt tggttcgctg ttgtaaaaag ctatatattt ttttataagg
     1561 ttatttttta tctgagcttc aaactcttcc tcttttggag agtagtagta ggtctttttc
     1621 cggccgtctt ccgttctgag tgtgctgtaa acaactatcg gggaaagggt tttaactttt
     1681 atttcttttt tcggaacgca gtccactgct gttattttat caattgttat gttctgccct
     1741 gaaatattta gtatctcttt ttcaaaaaca ccgtccagaa agtactttat aaaagcatca
     1801 tctatgcttg agatataaag atcgcaaaac tccttaaagg ttatcgagcg gtctttgatg
     1861 ttcatctcat aattcccgta aagtcttgaa aagacaaaca ttttgtactg tcttttttca
     1921 tagctgaatc cgttatcgtg tataaatttg ttatactttt cgtcattaat cagattcagt
     1981 attacggcct gaattatgtg gttgtactgg gtgggaagtt ttatgaaagg gttatcaata
     2041 tttctgagtc ttaattttat ttgtatggca acacttcctt tcttataatt ttatgataat
     2101 aaaattatat catttttcta tattttattt tcaaaaaaat ttataagtac atattaatgt
     2161 atcacttgat atattaataa aaaaacttcc ccttttcggg ggaagatttg ttattctttg
     2221 attactggat cacacaaaaa ggtaactgca agttctggcg gaactgcgct ggggttattc
     2281 tgataccatt cgagcgaagg tcttttttta tcaagtttcc agccgctaga gccaagccat
     2341 tcactgcgcc atttatccca caccggtcct atttcttcgc atgagcctac atggacaagc
     2401 acggcataaa ggcctccttc aaactggtat atgcttaatg gatctatatc agaaacttct
     2461 ttatgaaccg aaatcgatac gtcgtaacgt ttttgttcct gtggaatatc gtcggtatga
     2521 tcgtgaaaaa cagtcaggaa ctgtgcttct gtagaaaaaa gcttgtttgc acgaagtatt
     2581 tcatggaatt ttttccataa tgcaggcata ttccagtaat cgcctgttcc tcttaatccc
     2641 aagagtttca ttggctgtaa gtttacaatt ttaatttcca tctcattacc tccccgtggt
     2701 tttataaaaa agtagtacgg ttgtctttga ccgggactgt agtgtattcc cgcttgtgaa
     2761 taaagaagac ctttccagtc ttgcagcttt cttatccggc ttggacttat accatgagct
     2821 tttttgaatg ctttggtaaa ggcttcaagc gtttcatacc ctgattcaaa agctatctct
     2881 gttatggttt tatccgtccg taggatttca tatgcggctt tttccagtct gagccttttt
     2941 aggcaggctt ttacagtttc acccgtaagt ccttt
//
LOCUS       DAOSHR010000237         2951 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_31831_length_2951_cov_3.689227, whole genome shotgun sequence.
ACCESSION   DAOSHR010000237 DAOSHR010000000
VERSION     DAOSHR010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31831_length_2951_cov_3.689227"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /locus_tag="PLU28_10030"
     CDS             <1..704
                     /locus_tag="PLU28_10030"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOG35436.1"
                     /translation="QSELNPLDTYAVEQAVRIKKILPQSTVTAVSMGIPAAQAIVRQA
                     VAMGCDEGVLLSDRAFAGSDTWATAHVLAKGLQKLGGFDIIFAGERATDGETGQVGPC
                     VAVQLDIPVLTFVSEVKIEEDGKILATRSVEGGYELLRVSAPVLISVVKEINEPWIPD
                     YNGMMKAKSSSIKIMTAQDIGVDKTDCGLEGSPTRVSKVFYPKLTRNGEVFSDINPEK
                     SAQKILDFLKVKNIL"
     gene            715..1731
                     /locus_tag="PLU28_10035"
     CDS             715..1731
                     /locus_tag="PLU28_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOG35437.1"
                     /translation="MSKDIWTFARVINGRLTETSFELLTWGRSLADSLGSSLCSVFIS
                     SKIQNDEVDKLFLYGADKVYTVQDKKLENFIPDIYMKISCHMIKKYSPHVVIAPADTF
                     GKTLMPMIASKLRTGLTADCTDLSIEDETGLLMQTRPAIGGNVMATIKTPYTLPQMAT
                     VRPMAKKPLKHKGSTREIVEEIVGEEFFASCYEYVGFRQDESSEIALNQAEVIVGGGR
                     GMKDEKNFSRLYELAALLGGGVGASRAAVDMGWVTYPHQIGLSGKTVSPKVYFAFGIS
                     GAVQHIAGISGAEYVISVNKDPDSNIFNISDLSIIGDCNEVLEKLVKKLQETGAMIKN
                     ERIQ"
     gene            1715..>2951
                     /locus_tag="PLU28_10040"
     CDS             1715..>2951
                     /locus_tag="PLU28_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOG35438.1"
                     /translation="MKEYNKITSENIRKLRDICTDNAVIYEDLSALQAYSHDEAGGSF
                     YAHMPQAVVKPCSSEQVSQIVRFAASQNIPITPRGAGSGLAGACVPLYGGIVLSLERM
                     NKILEIDTQNMVAVVEPGVVTNDLCKKAQEKGLYYAGYPMSVHSSFIGGNVATNAGGS
                     KVIKYGNTSHHVLGLEVVLPDGSIVQYGGKRRKDSSGYDMVHLFIGSEGTLGVITKIF
                     LNLVPLAGMTVDLMVPFESVAKAIASIEPVIRSSSVLPSAVEFLDGTSVKYSCSYNNM
                     ELPFMQEAQAYMIVQFEAENSEQIQTLYEKAGKLFLDMGALNVYIADNRNNSEKIWRI
                     RRNWLESLKAIDPSVPTGDVVVPISEIPAMVEYIKAVGKKYEVDIPVAGHAADGNLHP
                     APLNNGSSCEQWREVSEKIL"
BASE COUNT          924 a          521 c          730 g          776 t
ORIGIN      
        1 tgcagtcaga gttgaatcct ttggatactt atgctgttga gcaggcggta agaataaaga
       61 aaatccttcc tcaaagcaca gtgacggctg taagtatggg aataccagcc gctcaggcta
      121 tcgtacgcca ggcagttgca atgggctgcg atgaaggtgt tctgctttca gatagagctt
      181 ttgccggttc agatacatgg gctacggcgc atgtactggc caaaggactg caaaagttgg
      241 gaggctttga cattatattt gcaggtgaaa gagctaccga cggagaaaca ggacaggtag
      301 gaccgtgtgt ggctgttcag ttggatattc cggttttgac ctttgtttcg gaagtcaaaa
      361 ttgaagaaga tggaaaaata cttgcaacca gatctgtgga aggcggctat gaacttctca
      421 gagtgtcagc cccggttctc ataagtgttg tcaaagaaat aaatgagccg tggattcctg
      481 attataacgg aatgatgaaa gcaaaatctt catccataaa gataatgaca gcacaggata
      541 taggagtgga taaaactgat tgtgggctgg aaggatctcc gacaagagtg agcaaagtat
      601 tttatcccaa gctgacaagg aacggagaag tattttcgga tatcaatcct gaaaaatcag
      661 cgcagaagat attagatttt ttaaaagtaa agaatatttt ataggaggaa taatatgagt
      721 aaagatatat ggacctttgc cagggtaatt aacggcaggc tgaccgaaac ttcattcgaa
      781 ctgctcacgt ggggaagatc tctggccgat tctctgggct catcactttg ttcggtgttt
      841 ataagttcta aaattcaaaa tgatgaagta gataagctgt ttttatacgg agcagataag
      901 gtgtatacag tacaggataa aaagctagaa aactttattc ccgatatata tatgaagatt
      961 tcatgccata tgataaaaaa atactctccg catgtagtta ttgcccctgc ggatactttt
     1021 ggcaaaaccc tcatgcctat gattgcctct aagcttagaa caggccttac agccgactgc
     1081 acggatcttt ccatagaaga tgagaccggt ttgctcatgc agaccagacc tgcaattggc
     1141 gggaatgtaa tggctacaat aaaaacacct tatacactgc ctcagatggc tacggtaagg
     1201 cctatggcaa aaaaaccgct taaacataaa ggatcaacca gagagatagt tgaggagatt
     1261 gtgggagaag aattttttgc aagctgctat gagtatgtag gattcaggca ggatgaaagt
     1321 tcagagattg ctttaaacca ggcagaagta attgtcgggg gaggcagggg tatgaaggac
     1381 gaaaaaaatt tttcccgatt atatgagctg gcagctcttc tgggcggagg agtcggtgct
     1441 tcaagagcgg cagttgacat gggatgggtt acttatccac atcagatagg actttccgga
     1501 aaaacggtga gtccaaaagt atactttgca tttggtatct ccggagcggt tcaacatatt
     1561 gcaggtattt ccggtgcaga gtatgtaatt tctgtaaata aagatccgga cagcaatata
     1621 ttcaatattt cggatctttc catcataggt gactgtaacg aagtgttgga aaagcttgtt
     1681 aaaaaacttc aagaaacagg agcgatgata aaaaatgaaa gaatacaata aaatcacatc
     1741 agaaaacata agaaaactca gagatatatg tacagacaat gcagtcatat atgaagatct
     1801 ttcagcactg caggcctata gtcacgatga ggcgggcgga agcttctatg cacatatgcc
     1861 ccaggcggta gtaaaacctt gttcgtccga acaggtttca cagattgtaa gatttgcagc
     1921 gtcacagaat atcccgataa ctcccagagg agccggaagc ggacttgcag gcgcatgcgt
     1981 tccgttgtac ggtggtattg ttcttagtct tgaaagaatg aacaaaatat tggagattga
     2041 tactcaaaat atggtagccg ttgtagaacc aggagtggtg actaatgatc tatgcaaaaa
     2101 agcccaggaa aaagggcttt attatgcagg atatccaatg agtgtccatt ccagctttat
     2161 aggcgggaac gtagcaacca acgcaggagg aagcaaagta ataaagtatg gaaatacttc
     2221 ccatcatgtt ctgggtctgg aagtagttct gccagacggt agcatagttc agtacggtgg
     2281 gaaaagacgg aaagactcaa gcggttatga tatggttcat ctttttatcg gatcagaggg
     2341 aaccttagga gttataacaa aaatttttct taatcttgtt ccgcttgcgg gtatgacagt
     2401 tgaccttatg gttccttttg aaagtgtagc aaaggccata gcctcaatag aacctgtgat
     2461 aaggagttcg tccgtgctgc ccagtgccgt tgagtttctt gacggcactt ctgtgaagta
     2521 tagctgttca tataataata tggagcttcc gtttatgcag gaggcacagg catatatgat
     2581 agtacagttt gaagctgaaa attcagagca gatccaaacg ctttatgaaa aagctggaaa
     2641 actttttctt gatatgggag cgttgaatgt ttatattgcc gataacagaa acaactccga
     2701 aaaaatatgg cgcataaggc ggaactggct cgaaagtctt aaagctattg atcctagtgt
     2761 tccgacaggt gatgttgtgg ttccgatatc tgaaattcct gctatggttg agtatataaa
     2821 agccgtagga aaaaagtacg aagtcgatat acctgttgct ggtcatgcgg cagatggcaa
     2881 tcttcaccct gctcccctta ataacggcag tagttgtgag caatggaggg aagtatcaga
     2941 aaaaattctt t
//
LOCUS       DAOSHR010000238         2948 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_31881_length_2948_cov_3.714829, whole genome shotgun sequence.
ACCESSION   DAOSHR010000238 DAOSHR010000000
VERSION     DAOSHR010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31881_length_2948_cov_3.714829"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="PLU28_10045"
     CDS             <1..302
                     /locus_tag="PLU28_10045"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOG35439.1"
                     /translation="SGDMLYTNVYDPKIFYTLNMKSKPVGRKKPNEIGLFDLFGNVKE
                     WCMTIPAKGKEAPVKGGSYISLESELSFESVALTDKETRSSELGFRIVRTVRTKN"
     gene            complement(299..2908)
                     /locus_tag="PLU28_10050"
     CDS             complement(299..2908)
                     /locus_tag="PLU28_10050"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOG35440.1"
                     /translation="MPKIIEYFFRTSNESEEFKKTKRIYIYLFVGILSLWCAFLPFTR
                     QVSEHGNLIIHSIVLCSLTVAGLILYLLKKHEIGVDIILATSGIYIIFYNGYFSDTVY
                     LAALMILSLLAFLLPGKRYQYFIFTAAVLIGVYIKYLRNISDENIVFSVMGLIFLLIM
                     FIVKENLNRITVSGSKLEEKILENQSISLQLEKAEQNVQRMRTMLNIGTWKYDMFEKI
                     FEGSSEFYKILGLSEKRKKVSVHEIFQKVIDEDKSLFRSIILKNGSDDIHKVEIRLRD
                     ESFGSKTVTMVMQREVNNIGVAVGITGVISDNACSRKPEIDFTMAHKIFEVSKDSIVI
                     SNGDDHVIYANKAYMENTGYSRNEIIGKKVKAFESPKNNVEIYNFIKRSVSQNYFWEG
                     EMWTRKKNGEDFMQLVSINLINNETDGGTYTVFVSRDLTEIMEKNESLEKLLKYDLLT
                     GLPNQNSIKERLCIELENMKKESMFMGFIVIDVKNFKRINDVLGHNEGDRILVQIAER
                     IAKFVVNIDCSSRLSSDEFGVIISGIHDLQEFLIRFESLNLVLNKPYFLNDKELDISF
                     KYGLSFFPDDSDDPRIIFQNAITALHYAKKSEESFYEFFTKEMSIRSTEKMQLEEEMR
                     QALINNEFRLVYQPQVDLKTKEIIGFEALVRWNSRNFGLIPPPKFIPLAEESKIIIDL
                     GDWILEQACLQNKKWQEIYGKRVVMAVNLSIIQFEERNFVEKIKKVLEKVRLRPEYLE
                     IEITEGIVAKNMEEVLKKLSELKNIGVRIAIDDFGTGYSSLSYIKKFPIDKIKIDRSF
                     IKDYPHYDNGSIAKTVIELSHNLDFSVIAEGAETEEQIEFLEKNNCDSVQGYYFFKPL
                     SESDVARLFENKN"
BASE COUNT          850 a          582 c          491 g         1025 t
ORIGIN      
        1 actccggaga catgctttat acaaatgttt atgatcctaa gattttttat actttaaata
       61 tgaaatcaaa accggtagga agaaaaaaac caaatgagat aggccttttc gatttgttcg
      121 gcaatgtaaa agagtggtgt atgaccattc ctgccaaagg taaagaagct cccgtaaagg
      181 gtggttctta tatttctctt gaatcggaac tttcctttga gtcggttgcc ctgaccgata
      241 aggaaactcg ttcttcagag cttggattca gaattgtacg gactgtcaga accaagaatt
      301 aatttttgtt ttcaaagagt ctagccacat cactttcaga cagaggctta aaaaagtaat
      361 agccctgtac tgaatcgcag ttgttttttt ccaaaaactc tatctgttct tcggtttctg
      421 caccttcggc tataactgaa aagtcgaggt tgtgcgaaag ttctataaca gttttggcaa
      481 tacttccgtt gtcatagtgc gggtaatctt ttataaagga tctgtctatt ttaatcttat
      541 ctataggaaa tttttttatg tagctcagcg atgagtaacc tgttccaaag tcatcaattg
      601 ctatgcgcac tcctatattt ttaagttcag aaagcttttt cagaacttct tccatattct
      661 ttgctacaat accttcagtt atttctattt cgagatactc cggtctgagt cgaacctttt
      721 cgagaacttt ttttatcttt tctacaaagt ttctttcttc aaattgtatt atcgaaaggt
      781 ttactgccat aacaaccctt ttaccgtaaa tttcctgcca ctttttattc tgaagacatg
      841 cttgttcaag tatccagtct ccaagatcta ttattatctt tgattcttct gcaagaggta
      901 taaacttcgg gggaggtata agtccgaaat ttctgctgtt ccatctcacc agtgcttcaa
      961 acccaattat ttctttggtt ttaagatcca cctgcggctg atatacgagc ctgaactcat
     1021 tatttataag tgcctgacgc atttcctctt caagctgcat cttttcggta gagcgtatgc
     1081 tcatttcttt ggtaaaaaac tcatagaagc tctcttcgga ttttttggca taatgcagtg
     1141 cggttatagc gttctgaaat atgatcctgg gatcatccga atcatccgga aaaaagctta
     1201 atccgtattt gaaggatata tcaagttctt tatcgttcaa gaagtacggc ttgttaagaa
     1261 caaggttgag ggattcaaac cttataagaa actcctgaag gtcatgtatg cctgaaatta
     1321 tgactccaaa ctcatcagag ctcaagcgtg atgagcagtc gatattaaca acaaattttg
     1381 ctatgcgttc ggcaatctgt actaatatcc tgtctccctc gttatgtcct aatacgtcgt
     1441 ttatgcgttt gaaatttttt acatctatta ctataaaccc cataaacata ctttcttttt
     1501 tcatgttttc caactcaatg caaagtctct cttttataga gttttgattg ggcaggcctg
     1561 tcaaaagatc gtatttaaga agcttttcaa ggctttcgtt tttttccatt atttcggtaa
     1621 ggtccctaga aacaaaaaca gtataggtac ctccatctgt ttcgttatta ataaggttta
     1681 tagaaaccag ctgcataaag tcttcgccgt tctttttcct tgtccacatt tcgccttccc
     1741 agaagtagtt ctgactgacc gaacgtttaa taaaattgta tatttcaacg ttatttttag
     1801 gggattcaaa tgctttgact tttttcccta ttatctcatt tctgctatat cctgtatttt
     1861 ccatgtaggc tttattggca taaatcacgt ggtcatcacc gttgcttatt actattgagt
     1921 cctttgaaac ttcaaatatt ttatgagcca tagtgaagtc tatttcgggc tttcgtgaac
     1981 atgcgttatc ggaaattact cctgtgatgc ctacggcaac acctatatta tttacttccc
     2041 tctgcatgac catagtaaca gttttactcc caaaagactc atctctcagt ctgatttcaa
     2101 ccttgtgtat atcgtcactt ccatttttaa gaataataga tctgaaaaga cttttgtctt
     2161 catcgataac tttctgaaaa atttcatgaa cacttacttt tttgcgtttt tcagagagac
     2221 ctaaaatttt atagaactcg gaagagcctt caaaaatttt ttcaaacata tcgtacttcc
     2281 aagttccgat atttagcatt gttctcattc tttgtacatt ttgttcggct ttttcgagtt
     2341 gcaaggagat actctgattt tcaaggattt tttcttcaag cttgctgccg ctaacggtta
     2401 ttctattaag attctccttt actatgaaca ttataagcaa aaaaataaga cccataactg
     2461 aaaaaacgat gttttcatct gatatgttcc gtaaatactt tatataaact cctatcagta
     2521 cagctgctgt aaaaataaag tactggtatc ttttgcccgg caacagaaaa gcaagcaggg
     2581 aaagaatcat caatgcagcc aggtagacgg tatcactgaa atatccatta taaaagataa
     2641 tgtatattcc acttgtggct aagattatat caactccgat ttcatgtttt tttaaaaggt
     2701 acagtataag tcctgccacc gtcagactgc aaagtactat agaatgaata attagatttc
     2761 catgctcgga aacttgtcgc gtgaaaggca gaaaagcaca ccataatgaa agtatgccca
     2821 caaacaggta tatatatata cgctttgtct ttttgaactc ttcagattcg ttggaagtac
     2881 ggaaaaaata ctctataatt ttgggcaacc ctcaccactt cctaaaaatc ggtctccctt
     2941 aagaggaa
//
LOCUS       DAOSHR010000239         2934 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_32090_length_2934_cov_4.937826, whole genome shotgun sequence.
ACCESSION   DAOSHR010000239 DAOSHR010000000
VERSION     DAOSHR010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 2934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2934
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32090_length_2934_cov_4.937826"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1012)
                     /locus_tag="PLU28_10055"
     CDS             complement(<1..1012)
                     /locus_tag="PLU28_10055"
                     /inference="COORDINATES: protein motif:HMM:NF016817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HOG35441.1"
                     /translation="MKNIVLMMFIVIISVSIFAVPFYSDVRPGYGVTEEKWLSDYFSG
                     LKDTWGDTKVYVLRGKEPGASALLLGGTHGNEISGIMAAMMYVERANVQKGTLYVIVR
                     ANNSNATYHEKLVAGTPDWVEFETQSGKRVVSYGSRRTNPMHQGNDPEKYRYPGTELE
                     FEGAEARNLNRVHPGNPDGTLTEKISYGIFELIRKENIDMVLDMHEAGTKSRLAYTLV
                     CNPKNIDMGAMVMLDLEQHDLGFKLEQSSKEFRGLSHKEIGDQTDAIAFLSETPNPGQ
                     DYGDYDGWSRQGLADVVNDAQYPLSHRVAVQVETFRLLCDYLPMFYEKEIIIQNIPSY
                     DEI"
     gene            complement(1056..1598)
                     /locus_tag="PLU28_10060"
     CDS             complement(1056..1598)
                     /locus_tag="PLU28_10060"
                     /inference="COORDINATES: protein motif:HMM:NF040376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6305 family protein"
                     /protein_id="HOG35442.1"
                     /translation="MKKIIAVITVIFALALVWAGDYPVFSGPLLITSAGQSAGAVQLK
                     VLSTKAKIKFEYDGVAKAEKLSSFKSVMIVVGASTKGLGAAGLDNDKEMARVQTILDK
                     AKELGIPVILAHIEGASRRGSTSDKLAELIAPYSSLYIIVNSGNEDGKFNKLAEPKKT
                     PIAFFDKVSAIQPILSEIIK"
     gene            complement(1628..>2934)
                     /locus_tag="PLU28_10065"
     CDS             complement(1628..>2934)
                     /locus_tag="PLU28_10065"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HOG35443.1"
                     /translation="APTIKLAREGFVISTTFAQTLSDNYKKLLDDPGEGTDIFTNEGL
                     LYNEGDIFKNPVLADTLEILAKGGIDAFYKGEIADKIADFYAKNGGAITKEDLAGYKA
                     IVKEPVVGTYRGYKVITAPPPVGGVTLVEVLNILENFNLSKFSWDDPLFVHLVADSIF
                     LGQVDKRMYLGDAPNMPVKGLLSKEYAKSMFVKIPLDKALPRKDKIDVLTMAGEPMLY
                     ENNETFLENILAKANQKSVASEVVYESPSTTHYSVVDKDGNAVSVTQTLSSFFGGGNV
                     IPGTGFAMNNEMQNFSTYSGSPNELISGRRPLTIIVPTIVENPEGEVLMIAGSPGSSR
                     ITSTLIQIIVNVVDLKMPVALAMKTPKFTTYDTEDSLTIEGGYSKSTIDALKNYYGYK
                     IIEKGPLDLYFGGPNIVYRYSDGTLLGIGSFRREGGASAPQW"
BASE COUNT          805 a          680 c          507 g          942 t
ORIGIN      
        1 atatttcatc gtatgagggt atattttgaa taattatctc cttttcatag aacatcggaa
       61 gataatcgca caaaagcctg aatgtttcaa cctgtacggc tactctgtgg ctcagaggat
      121 actgggcatc atttacaaca tctgcaagac cctgccttga ccaaccatca taatctccat
      181 aatcctgtcc gggattagga gtttccgaaa gaaaagctat tgcatctgtc tgatcaccta
      241 tctctttatg agaaagccct ctgaactcct tgctggactg ctcaagctta aaaccaagat
      301 catgctgttc aagatccagc ataaccattg ctcccatatc tatattctta ggattacata
      361 cgagagtata cgctaatctt gactttgtac cagcctcatg catatcaagc accatatcta
      421 tattttcttt tcttataagc tcgaatatgc cgtaacttat cttttccgta agtgttccat
      481 caggatttcc cggatggact ctattcagat ttcttgcctc ggcaccttca aattccagtt
      541 cagttcctgg atatctatat ttttcaggat catttccctg gtgcatcgga ttggttcttc
      601 tgcttccata acttaccact ctttttccgc tctgcgtttc aaactctacc caatcaggag
      661 ttccggcaac aagtttttca tgataagtgg cattactgtt gttagccctt actattacat
      721 aaagcgtacc tttctgaaca ttagctcttt caacatacat catggctgcc attatccctg
      781 aaatctcatt accatgagtg cctccgagca gtaatgcact tgctccgggt tcttttcctc
      841 taagcacata tacttttgta tcgccccacg tatccttaag accggaaaaa tagtctgaaa
      901 gccatttttc ttctgtcaca ccgtagcctg gccttacgtc gctgtaaaaa ggaacagcaa
      961 aaattgaaac tgatattatt actataaaca tcattaatac aatgtttttc atttattctc
     1021 ctcctaaaaa acatgggggc acttggcccc ctattctatt ttattatttc tgaaagtata
     1081 ggctgtatgg ctgaaacctt atcaaaaaat gcgataggag ttttctttgg ttcagcaagc
     1141 ttattgaact tcccgtcttc atttcctgaa ttcacaatta tatagagtga tgagtacgga
     1201 gctattaact ctgcgagctt gtcacttgta gaacctcttc ttgaagctcc ttcgatatga
     1261 gcgagaataa ccggaatacc caactctttt gctttatcaa gtatagtctg aactcttgcc
     1321 atttccttgt cattgtcaag tcctgcagca ccaagtcctt ttgtgcttgc gccgaccaca
     1381 atcattaccg atttgaatga tgaaagcttt tctgcttttg ccacaccatc atactcaaac
     1441 tttatctttg ctttggtaga aagaacttta agctgtacgg ctcctgcact ctgacccgca
     1501 gaggttatga gcagcggacc tgaaaaaacc gggtaatcgc ccgcccaaac caaggctaaa
     1561 gcaaaaatta ccgtaataac tgctataatt tttttcatca aaatcactcc ttatctataa
     1621 ttgcttttta ccactgaggt gcggaagctc cgccttctct tctgaatgaa cctattccaa
     1681 gaagggttcc gtcagagtat ctgtatacta tgttcggacc tccaaaataa aggtcaagcg
     1741 gtcctttctc aattatttta tatccgtaat aatttttaag tgcatctatt gttgacttag
     1801 aatatccgcc ttcgatggtc agcgaatctt cggtatcgta tgtcgtaaat ttaggtgtct
     1861 tcattgccaa agccaccggc atcttcagat caaccacatt tacgattatc tgaataagag
     1921 tagaagttat ccttgaagaa ccaggtgaac cggctatcat aagaacctcg ccttctggat
     1981 tttccactat tgttggaact attattgtaa gcggccttct tccggaaata agttcattcg
     2041 gacttccgga atatgtgctg aagttctgca tctcattgtt catagcaaag cctgttccgg
     2101 gaataacgtt tcctcctccg aagaaagaac taagggtctg ggttaccgat actgcatttc
     2161 catctttgtc aaccacgctg taatgagtcg tggaaggaga ttcatagact acttcacttg
     2221 caacagactt ttgatttgct tttgcaagaa tattctcaag gaatgtttca ttgttttcat
     2281 aaagcatagg ttcacccgcc atggtaagca cgtcgatctt atcttttctg ggaagagctt
     2341 tgtccagagg aatctttaca aacattgact tagcatattc ctttgaaagc agtcctttta
     2401 ccggcatatt cggagcatca cccagataca ttctcttatc aacctgacca aggaatattg
     2461 aatcagcaac caagtgaaca aataacggat cgtcccagct gaatttcgac agattaaagt
     2521 tctcaagaat gtttaaaact tcaacaagag ttactccacc tactggaggt ggtgcagtaa
     2581 taaccttata acctctgtaa gttcctacaa ccggttcttt tacaatggct ttatatccgg
     2641 caagatcttc tttggttata gcgccgccgt ttttggcata aaaatcagca attttatccg
     2701 ctatttctcc tttataaaaa gcatctatac cgccttttgc aagaatttca agcgtatcgg
     2761 caagcactgg atttttaaat atatctcctt cgttatacaa aagaccttcg ttggtgaaga
     2821 tgtctgtacc ttcgccagga tcatcaagaa gcttcttata attatccgat aacgtctgtg
     2881 caaaggtagt gcttattaca aacccttcac gtgccagctt tattgtagga gcaa
//
LOCUS       DAOSHR010000240         2925 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_32248_length_2925_cov_4.056794, whole genome shotgun sequence.
ACCESSION   DAOSHR010000240 DAOSHR010000000
VERSION     DAOSHR010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32248_length_2925_cov_4.056794"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2015
                     /locus_tag="PLU28_10070"
     CDS             <1..2015
                     /locus_tag="PLU28_10070"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOG35444.1"
                     /translation="AQQDITTIPLIDWEDISSGDLIVHEDFGIGIYRGFEKKKTALGV
                     REYIILEYESSTKTLIPVERIDKLSKYIGDAQTVKISTPGGARWKNTKKKVEENIRER
                     INELVKIYAIRQEQKGLIIEGDIETERKFRETFPYIETADQEKAIEQIERDLSSEKTM
                     DRLLVGDAGFGKTEVAMRAAFKTVLSDYQVLMLAPTTILAKQHFDSFYERMNEFGVRI
                     ELLDRYKTQREKEEVYKQVRDGVVDIVIGTHALFSKKLIFKKLGLVIIDEEQRFGVLQ
                     KERFKSISEGVNFLLMSATPIPRTLYMSVSGLRDISTISTPPIGRLPVQTFVGSYSER
                     IVRMAVLREKLRGGQILYVHNRVQDIDKIYRKLKDAIPEVSIACAHGRMKKSEFSKIL
                     QEFYSGKLDMLVATTIVENGIDIPNVNTLIVDDSQRYGISQLYQIKGRVGRSNKRAFA
                     YFMHTSSALNNDARKRLEAIKEFNEPGSGLRLALRDLEIRGYGDILGLDQKGHINSVG
                     LQLYKSILDRVLSQLYNDKDIVRDSDFVTEIKGIKGSIIIPEEYIENPIERMRIYRRI
                     SVARNIIEIDELYSEIADRFGRMPLETERLIEYARIKVLAASKNIREIEIGDAYIKMK
                     YVESFEPDPLKIRKFSKKSFQDKKNEEYIVYGNFDEVKFLKEILGE"
     gene            2020..>2925
                     /gene="mnmE"
                     /locus_tag="PLU28_10075"
     CDS             2020..>2925
                     /gene="mnmE"
                     /locus_tag="PLU28_10075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HOG35445.1"
                     /translation="MIFDTITAISSPMGQGAVAVVRVSGSESRNIAEKIFNKKNPSIK
                     HMYYGMLYDGSILIDEVTWVFHESPSSFTGEDMLEVFCHGGRLITQKVFETIIKSGAR
                     QALPGEFSFRAVLNGKMDLIKAESINDMINAKTDNALKVASANLSNGISAQIKEIKEM
                     LMYFAASIEVELDYPEDFEQDDSSEKISYLKQIISKTGNILKNAQNGIAAVQGVKTCI
                     VGKPNSGKSTLLNSLLKTERAIVSPFAGTTRDTIEENISLFGINIRLIDTAGIRQTDN
                     EIENLGIVRSKKAIEQSEMIIFVIDG"
BASE COUNT         1045 a          442 c          657 g          781 t
ORIGIN      
        1 atgctcagca ggatataaca actattccgc ttatagactg ggaagatatc tcatcgggcg
       61 atcttattgt gcatgaggat ttcgggatag gtatatacag aggatttgaa aaaaagaaaa
      121 ccgcacttgg ggtacgtgaa tatataattc tggagtatga aagttctacc aaaacgctta
      181 ttcctgttga acgtatagat aagctttcca agtatatagg tgatgctcag accgtaaaaa
      241 tatcaactcc cgggggagca agatggaaga atactaagaa aaaagttgag gaaaatataa
      301 gagaaagaat aaatgaactg gttaaaatat atgctatacg tcaggaacag aaaggcctga
      361 ttatagaagg agatattgaa accgaaagaa agttcagaga gacctttccg tatattgaaa
      421 ctgcagatca agaaaaagca atagagcaga ttgaacgcga tctcagctca gaaaaaacga
      481 tggacaggct tttagttgga gatgcaggat ttggaaaaac agaagttgca atgagagcag
      541 catttaaaac ggtgctttct gactatcagg tacttatgct agcgcctacc actatattag
      601 ccaaacagca ttttgacagc ttctatgaac gtatgaatga gtttggggta aggatagagc
      661 ttcttgacag gtataaaacc caacgtgaaa aagaagaagt ttataagcaa gtaagagatg
      721 gagttgttga tatagttata ggcacacatg cgctattttc aaaaaagctt atcttcaaaa
      781 aactaggatt agtcataata gatgaagagc aaaggttcgg agttctacaa aaagaaaggt
      841 ttaaaagcat ttccgaagga gtgaactttt tgcttatgag cgcaactcct attccgcgta
      901 cattatatat gtctgtaagc ggattacgag atatttcgac cataagtact cctcctatag
      961 gtagactacc ggttcaaact tttgtcggga gctattccga gcggatagtc agaatggctg
     1021 tattgagaga aaaacttaga ggcggacaga tactttatgt gcataacaga gttcaggata
     1081 tagataaaat atacagaaag cttaaagatg ctatacctga agtaagtata gcctgtgctc
     1141 acggaagaat gaaaaagagt gagttttcca agatacttca ggagttctat tccggtaagc
     1201 ttgatatgct tgttgcgaca accatagttg aaaacggtat agatattcca aacgtcaata
     1261 ccctaattgt agatgattct caaagatatg gaatatcgca gctttaccag ataaagggaa
     1321 gagttggaag gtctaacaaa agagcctttg cttactttat gcacaccagt tcggctctta
     1381 ataatgatgc aaggaaacga ctggaagcca ttaaagaatt taatgaaccg ggaagcggtc
     1441 tcagacttgc actgagagat ctggagatac gcggatatgg agatattctt gggcttgatc
     1501 aaaaaggtca tatcaactct gtcggacttc agctttacaa aagtattctt gacagagttt
     1561 taagtcaact ttacaacgat aaggatatag ttagggacag cgactttgtt acagagatta
     1621 aaggtataaa aggttctata ataattcccg aggagtatat tgaaaatcca attgagagaa
     1681 tgcgtatata cagacgaata tcggtagcga ggaatatcat agagattgat gaactataca
     1741 gtgagatagc tgatagattt ggtcgtatgc ctcttgaaac agaaagactt attgagtacg
     1801 caaggataaa agtactggca gcatcgaaaa atatcagaga gatagagata ggcgatgctt
     1861 acataaagat gaagtatgtg gagagttttg agccagatcc gcttaaaatc cgtaagtttt
     1921 cgaaaaaaag ttttcaggac aaaaagaatg aagagtatat agtatacggt aactttgatg
     1981 aagtgaaatt tcttaaagaa attctcggag agtgattcta tgatctttga tacaataacc
     2041 gcaatttcgt ctcccatggg tcagggagcg gtagctgtcg tgagagtaag cggaagtgaa
     2101 agtcgcaata tagctgaaaa aattttcaat aaaaaaaatc ccagtattaa gcatatgtac
     2161 tatggaatgc tttatgacgg ttcgattttg atagacgaag taacatgggt ttttcatgaa
     2221 agtccctcta gttttaccgg agaagatatg cttgaagtat tttgtcatgg gggaagactt
     2281 attacccaaa aggtttttga aacaataatt aaatcaggtg caagacaggc gctgccagga
     2341 gagttttctt ttagggctgt tcttaacggc aagatggatc tcataaaagc ggagagcatt
     2401 aacgatatga taaacgcaaa aaccgataat gccctcaaag ttgcatcagc aaatctcagt
     2461 aatgggattt ctgctcagat taaagaaata aaagagatgc tgatgtactt tgccgcatcc
     2521 atagaggtag aacttgatta tccagaagat tttgaacaag atgacagtag tgagaagata
     2581 tcctatctca aacaaattat ctctaaaaca ggaaatattt taaagaatgc gcaaaatggg
     2641 atagccgctg ttcagggtgt aaaaacctgt atcgtaggaa agccgaactc tgggaaaagt
     2701 acgcttttaa actcactttt aaaaacagaa agggcaattg tgtcgccatt tgctggaact
     2761 acaagagata ctattgagga aaatataagt ctttttggta taaacatcag acttatcgat
     2821 actgccggaa taagacagac tgataatgag attgaaaatc ttggtatagt acgttcaaaa
     2881 aaagcaatag agcaatcaga gatgattatt tttgttatcg atggt
//
LOCUS       DAOSHR010000241         2902 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_32624_length_2902_cov_3.441166, whole genome shotgun sequence.
ACCESSION   DAOSHR010000241 DAOSHR010000000
VERSION     DAOSHR010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 2902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2902
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32624_length_2902_cov_3.441166"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1214)
                     /locus_tag="PLU28_10080"
     CDS             complement(<1..1214)
                     /locus_tag="PLU28_10080"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HOG35446.1"
                     /translation="MKKICFFVAVLFMIAAVYGYDIRKGDVLGVWVYGYPDLTSSSIY
                     VGPQGEITIPPLGRIKAEGMSIEALQELLEQKMQEYIRSARVTVGVVQYAPFKVSVFG
                     NTKVQGYVDIKSESVRLSELVASLGGLADPMKTSYVLIRYPDGKESKVSMDWIFNGTK
                     GDDPLITENSYVLFPLEYTNNVSVFTEQISKSLEYRNGMTLSDIISGLGISISQVDDS
                     ITVLNSKEIKSYSLRSVIEGEKVFLEKGDTVIINRLKKFVYLFSPAFTGKMDFEKNEP
                     MSLKKVILSVNLQLSQANDMITVIRGSQKESYSLERVINGFDVELQSGDVIQLEKYSK
                     YVYLFSSAFSGKMDFEKSDEMTMKKLIAKSGIVTDQYEDNVVVIRDSLKLEYQIQQIK
                     QGGDFQLLNGDIV"
     gene            complement(1232..1693)
                     /locus_tag="PLU28_10085"
     CDS             complement(1232..1693)
                     /locus_tag="PLU28_10085"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG35447.1"
                     /translation="MENRSIQESNVYNNIANKHLENGEYEKALSFFLKAISLNSENSV
                     LYHNAGVCLMLMNEYERAVSLFEKALEKKIHLSETRFYRIQCLYEAGFYKKVIDAELP
                     FDNNQKLECLVLQIRAAIKNGDPVTAKDILKKIKMMGYNSQELEFLENMIG"
     gene            complement(1683..>2902)
                     /locus_tag="PLU28_10090"
     CDS             complement(1683..>2902)
                     /locus_tag="PLU28_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35448.1"
                     /translation="GTDKGIYYMEPFSDYITFESQQASPVENEVKLKELRAYENNLKP
                     LLEGFPAVIFGLDKLGRVVAYDPSKNDLMIIGFDGTVLVKYKFSDKEVLLNGGIWQNY
                     LYVHEGISNSIYIYDMTDNKILKKYPLDITGVQSLAVLPWGMPLISSKDGVFIFNLLN
                     LKSFPLFDKKSQFGSFRGILRLKNGVAVFLDQKKYTATLCRFSSETQYQPYVMNVYGI
                     NVNDLQIRAKFSVTDISGNKTDFITKDLLVFDSGGRMSFEYEREYSKADIYEIKGVDQ
                     FFSSALSQIKTDSFVILSGTLASKLNQSQSVPLILSGCTVFYVNDNSESIDPTLRDII
                     YSTGGYILRSSDVSYLKRYLSGSYKLVDYVEYKLVAPIVEGIKQTKLSVILPGGVLSD
                     SLYYYSEGVGVGK"
BASE COUNT          856 a          617 c          489 g          940 t
ORIGIN      
        1 actatatctc cgtttaggag ctggaaatct ccaccctgtt ttatctgctg tatctgatac
       61 tcaagcttaa gactgtctct tatgacaaca acgttatcct cgtattggtc tgttacgatt
      121 ccggactttg caataagctt tttcatagtc atttcatcac ttttttcaaa gtccatcttt
      181 cctgagaatg cagatgaaaa aagatacaca tacttactgt acttctcaag ctgaataaca
      241 tctccgctct gcagctcaac atcaaaaccg tttatgactc tttccagcga gtagctttct
      301 ttttgggaac cgcgtataac tgtaatcata tcgtttgcct gagaaagctg taggttgacg
      361 cttaagataa cttttttaag actcatgggt tcattttttt caaagtccat ctttcctgta
      421 aaagccggag agaatagata tacaaatttt ttaagtctgt taattattac ggtatctccc
      481 ttttcaagaa agactttttc gccttcgatg acagatctga gggagtacga ttttatttcc
      541 ttgctgttaa gcactgtaat gctgtcatct acctgtgaaa tagagatacc cagccccgaa
      601 atgatatccg aaagagtcat tccgtttctg tactctagtg attttgagat ctgttcagta
      661 aatacagaca cattattggt gtattcaaga ggaaaaagca catatgagtt ttcggttatc
      721 agaggatcat cacctttggt accgttaaag atccaatcca tagaaacctt agactcttta
      781 ccgtcggggt accttataag cacataggag gttttcatcg gatcggcaag tccgcctaaa
      841 gaggctacca actccgaaag tcttacagat tcagatttta tatcaacgta tccctgcacc
      901 ttggtattgc caaagacact aaccttaaat ggggcatact gcaccactcc tacggttact
      961 cttgctgaac gtatatactc ctgcatcttt tgttcaagaa gctcctgaag agcttcaata
     1021 ctcattcctt ctgcttttat ccttcccaga ggaggaatgg ttatttctcc ctgcggacca
     1081 acatatatgg agcttgaggt aaggtcagga tatccgtata cccacactcc cagtacatcg
     1141 ccttttctta tatcgtatcc gtagactgcg gctatcataa aaagtaccgc aacaaaaaaa
     1201 caaatttttt tcattcgtcg tacctcctga atcatcctat catattttct aaaaattcta
     1261 attcctgaga attatatccc atcatcttta tctttttcag aatatctttt gctgtaacag
     1321 gatctccgtt ttttatagct gctctgatct gaagaacaag acattccaac ttctgattgt
     1381 tatcaaacgg aagctctgca tcaataactt ttttataaaa acccgcctca taaagacact
     1441 gaatacggta aaatcttgtc tcactgagat gtatcttttt ttcaagagct ttttcaaaaa
     1501 ggctgaccgc tctttcatat tcattcataa gcatgaggca tactcccgca ttatggtaaa
     1561 gcactgaatt ttcactattt agagaaatag ctttaagaaa aaaagaaagt gccttttcgt
     1621 actctccgtt ttcaaggtgt ttgttagcta tgttgttata gacattggat tcttgaatac
     1681 ttctattttc caacgccaac accttccgaa tagtagtata gtgaatcgct cagtactccc
     1741 cccggaagaa taacggaaag tttggtctgt tttattcctt cgactatggg agcaacaagt
     1801 ttatactcca cataatccac aagtttgtat gaaccggaaa gatacctttt aagatagctt
     1861 acatccgagg aacgcagaat gtatcctccc gtactataga ttatgtccct cagcgtggga
     1921 tctatacttt cggagttatc gtttacataa aataccgtac accctgaaag aataagtgga
     1981 acagactggg actggttgag tttggaagcg agcgtcccgc tgagaatgac aaacgaatct
     2041 gtttttatct gtgaaagtgc cgaagaaaaa aactgatcga ctccctttat ttcatatata
     2101 tcagcttttg aatactctct ttcatattca aagctcatcc ttccgccgga atcaaaaacc
     2161 agaagatctt tggtaatgaa atcggtctta tttcctgata tgtcggtaac actaaattta
     2221 gctcttattt gaagatcgtt tacatttatc ccataaacat tcattacata cggctgatac
     2281 tgtgtttcgc tgctgaatct gcaaagagtg gcagtatatt ttttttgatc caaaaaaaca
     2341 gcaacaccgt ttttcagtct caaaattcct ctaaaagaac caaactggct ttttttgtca
     2401 aaaagaggaa aggattttaa attaagaagg ttaaagataa aaactccatc tttggaagaa
     2461 ataagaggca ttccccatgg taagaccgcc aaagactgta ccccagttat atccaaagga
     2521 tattttttta agattttatt gtctgtcata tcataaatgt agatgctgtt gcttattcct
     2581 tcgtgaacat aaagatagtt ctgccatatt ccaccgttta aaagcacttc tttatctgaa
     2641 aatttgtact ttacaagtac tgtaccgtca aaaccgatta tcatgaggtc atttttagag
     2701 ggatcataag ctactaccct tccaagcttg tccagtccga atattacggc aggaaagcct
     2761 tccaaaagag gcttaagatt attttcataa gctctgagct ctttaagctt tacttcgttt
     2821 tccacaggac ttgcctgttg actctcgaag gtaatataat ccgaaaaagg ctccatataa
     2881 tatattccct tgtcagttcc ca
//
LOCUS       DAOSHR010000242         2885 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_32913_length_2885_cov_3.893993, whole genome shotgun sequence.
ACCESSION   DAOSHR010000242 DAOSHR010000000
VERSION     DAOSHR010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32913_length_2885_cov_3.893993"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..950)
                     /locus_tag="PLU28_10095"
     CDS             complement(<1..950)
                     /locus_tag="PLU28_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HOG35449.1"
                     /translation="MTVYQINGPVVIATGATDTFLNEMVKVGNKKLTGEVIAINGDMA
                     TIQVYEETSMLKPGEVILPSGKMLSAVLGPGMIGNIYDGIQRPLKELYSMSGDFIGRG
                     LDAWPIDIQRIWDVQIFKDIGDKVSEGEIYAALKESPSIEHRLLVPQGVNGIVKSVEK
                     SSRHKVTDTLMTVETDETTVELKMYQEHPVRIPRKLKEQYSTNTPLITGQRAIDIFFP
                     ISKGGTAAIPGGFGTGKTMTQHQLAKWADADVIVYIGCGERGNEMTDVLTDFPKLIDE
                     RNGRPLIERTVMIANTSNMPVPAREVSIYTGVTIAEYYRD"
     gene            complement(947..1525)
                     /locus_tag="PLU28_10100"
     CDS             complement(947..1525)
                     /locus_tag="PLU28_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35450.1"
                     /translation="MIQMDEKVQKLLNLIQTKYHTDEEALKKQYEKRISELEKKYEKD
                     IQTETIEIYLNEQETVRTIEKKGFRESEMFYNKEKLKLINTFIERLMAEIKEKIINSN
                     FEKKRNIYLLLFNQALLLTKDVYTISCSKDDYELIKSFSEDKKILIDSTLSGSIVLDC
                     GKYKIKSTIDMFLNQYTPMIYDFVIKWMEEYR"
     gene            complement(1509..1832)
                     /locus_tag="PLU28_10105"
     CDS             complement(1509..1832)
                     /locus_tag="PLU28_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HOG35451.1"
                     /translation="MKYFLLSDNIDTHVGMNLAGVEGVIVHSVPEALKAFSDIIKDKD
                     IGVLLITELVQSFIKEEIKIHKLSGKIPLIFIIPDRHGWRGDKNFITSHVENAIGVKI
                     NDTDG"
     gene            complement(1834..2265)
                     /locus_tag="PLU28_10110"
     CDS             complement(1834..2265)
                     /locus_tag="PLU28_10110"
                     /inference="COORDINATES: protein motif:HMM:NF012365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit C"
                     /protein_id="HOG35452.1"
                     /translation="MVGLITVSCVFLFTVGTGIFLGKKPHLAKKIGKLILKINITVFF
                     FAVFMSAFLLFPSTGFSQEQTVQTASASSVSSMAFLAAAVAVSASSIGAAIAVAAVGS
                     AAMGAISEKPELFGRSILYVGLAEGIAIYGLIIAIMILGKV"
     gene            complement(2285..>2885)
                     /locus_tag="PLU28_10115"
     CDS             complement(2285..>2885)
                     /locus_tag="PLU28_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HOG35453.1"
                     /translation="YNINYFLVTSIVIGFVIIITARLTHTVNMFIIGKAKEAVFDPNG
                     ISGFGLYIIILWAAVYFFMTGKFLYFLIYPAGVFVFMILLKEIIFGEGSFLMRFIQGF
                     FELFEVLISYLTNTLSFIRLGAFALNHAGLFMAFYMMSQMTQNKGLSFLILVLGNILI
                     IVLEGVIVFIQALRLQMYEYFSRFYNGNGRFFNPDRYKL"
BASE COUNT          883 a          582 c          454 g          966 t
ORIGIN      
        1 atgtcccggt aatattcggc gatggttacc cccgtgtaga tactgacttc acgcgcggga
       61 accggcatgt tggaggtgtt ggcaatcatc acggttcttt cgatcagcgg ccgtccattg
      121 cgctcatcaa tcagttttgg aaaatccgta agaacgtcgg tcatttcatt ccctcgttct
      181 ccgcagccga tataaactat tacatcagca tctgcccatt tagcaagttg atgttgagtc
      241 atggtttttc ctgttccgaa tcctcctggt atggcagcag ttccaccttt acttatcggg
      301 aaaaaaatat ctattgctct ttgtccggta ataagtgggg tatttgttga atactgttct
      361 tttaactttc taggtatcct taccggatgc tcctgataca ttttcaattc aactgttgtt
      421 tcgtctgttt ctacagtcat tagtgtatct gtaactttgt gtcgcgaaga tttttccaca
      481 gatttaacta ttccattaac tccttggggt accaaaagcc tgtgttcaat gctgggactt
      541 tctttcaaag ctgcatagat ttctccctca gaaactttgt caccaatatc tttgaatatc
      601 tgcacatccc atatcctttg aatgtcaatt ggccatgcat caagacctct tccaatgaaa
      661 tcacccgaca tagagtacag ttctttcaaa ggacgttgta ttccgtcata aatatttcca
      721 atcattccag gtcccaaaac agcactaagc atcttcccgc ttggtaatat aacttctccc
      781 ggtttaagca ttgaagtttc ttcataaact tgtattgttg ccatatctcc atttatagcg
      841 attacttctc cggtaagttt tttgttgcca acttttacca tttcattaag aaaagtatct
      901 gttgctccag tggcaataac aacaggacca tttatctgat aaactgtcat cggtattcct
      961 ccatccattt tataacaaaa tcatatatca ttggagtata ctggttcaaa aacatatcta
     1021 tggtgctttt gatcttgtat ttgccacaat caagtactat agagcccgaa agtgtactgt
     1081 caataagtat ttttttatct tccgaaaaac tttttataag ctcataatca tctttgctgc
     1141 aagatatagt gtatacatct tttgtaagca atagagcctg gttaaaaagg agaagatata
     1201 tatttctttt cttttcaaaa ttgctgttaa tgatcttttc tttaatctca gccataagtc
     1261 tttctataaa agtattaatg agttttaatt tttctttatt atagaacatt tcagattcac
     1321 gaaagccttt cttttcgata gttctcacag tttcttgttc atttagatat atttcaattg
     1381 tttctgtttg aatatctttt tcatattttt tttccaattc agatattctt ttttcatact
     1441 gcttttttaa agcttcttcg tctgtgtgat actttgtttg tataagattc agaagtttct
     1501 gaactttctc atccatctgt atcatttatt ttcactccaa tagcattctc tacatgggat
     1561 gtaatgaaat ttttatctcc gcgccaccca tgtctatccg gaataataaa tataagtgga
     1621 atttttcccg aaagtttgtg aatttttatt tcttccttaa taaaagactg tactagctct
     1681 gttatcagga gtactccaat atctttatct tttataatgt ccgaaaaagc ttttaacgcc
     1741 tctggtacac tgtgtacaat aacaccttca actcctgcaa gattcattcc aacatgagta
     1801 tcgatattat cacttagtaa aaagtacttc atattaaacc tttcccagaa tcattattgc
     1861 tattatcagt ccatatatag ctattccttc tgccaaacca acgtatagaa ttgatctgcc
     1921 aaaaagttcc ggtttttcac ttatagcgcc cattgctgca gagccaacag ctgctactgc
     1981 aattgcagca cctatagaag atgctgacac ggctactgct gcagctaaaa atgccatgga
     2041 ggaaacagaa gatgccgaag ctgtctgaac cgtctgttcc tgcgagaagc ctgttgaagg
     2101 aaatagaaga aaagccgaca taaaaacagc aaaaaagaag actgtaatgt ttattttaag
     2161 aattaactta ccgatctttt tcgcaagatg cggtttttta cccaagaaga taccagttcc
     2221 tactgtaaaa aggaaaacac atgaaaccgt tatcagacca accatatccc acctccgatg
     2281 agtgttagag tttatatctg tcaggattaa aaaatcttcc atttccgtta tagaatctcg
     2341 aaaagtattc gtacatctga agacgcagag cctgaataaa aacaatcaca ccttcaagaa
     2401 cgattataag gatattgcct aatacaagga ttaaaaaaga gagtccttta ttctgagtca
     2461 tttgtgacat catataaaat gccataaaaa gtcctgcatg attaagggca aaagcaccaa
     2521 ggcgtataaa tgaaagggta tttgtaagat acgatatcag cacttcaaaa agttcaaaaa
     2581 atccttgaat gaagcgcatc aaaaatgacc cttctccaaa aataatttct ttaagtaaaa
     2641 tcataaatac aaatactccg gcaggataga taagaaaata caaaaacttt cctgtcataa
     2701 aaaaatatac ggcagcccat aaaatgataa tgtaaagtcc aaatccgctt atgccgttag
     2761 gatcaaacac agcttctttt gcttttccta taataaacat gttaactgtg tgagtaagcc
     2821 tggctgtaat tataattaca aaacctatta cgatagatgt aaccaagaag taattgatat
     2881 tatac
//
LOCUS       DAOSHR010000243         2829 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_33857_length_2829_cov_5.070656, whole genome shotgun sequence.
ACCESSION   DAOSHR010000243 DAOSHR010000000
VERSION     DAOSHR010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33857_length_2829_cov_5.070656"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(68..595)
                     /locus_tag="PLU28_10120"
     CDS             complement(68..595)
                     /locus_tag="PLU28_10120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOG35454.1"
                     /translation="MIDPLYKNMEMKIIFSLLENSEQNVNELMRSLKTGAGSTLKAIN
                     DLEDLGIIKKEKRANAHFYSLNKSFSPLEAFFNSLFMVNKAFNDEFDIFIKKIFSAYP
                     LTLSIFENPKIINSFTCVMKKTQPFTETFEGAELRFLGLKDFFNDVQKYRDLVCHGLS
                     IARLEQLDSLKSYFE"
     gene            752..2083
                     /locus_tag="PLU28_10125"
     CDS             752..2083
                     /locus_tag="PLU28_10125"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOG35455.1"
                     /translation="MKKYLLILMVFIFSSVLYTRQIEVWHTYGGCKKEFLQKTCTYYA
                     QDNTQVTIKLVGFSDYASMMTALNLSIKSSIPPDVLICPDSLSKILIDSGAVLTVQKF
                     IDTDTDFSQKNLFPFILEKFKYKQTIQALPFNPVILMSFYNSSLSKKLGLDSTSIPST
                     LSEILQNAKSAAMSELYIQEDISYVSMPLDSIMLENFLCMTNLPFTDHDNGRSESPDS
                     IMPDSSQIYSFLDFWLSMQNLGYAKLYGYDSWQEAMDSFINGKTCLTFSWSDNIKSIL
                     NQSEKRKFKTALGFVPVFKRMEGGPLVSCEGLWLVSGHTQDEIKETWNFLKFLVSEKI
                     QAECFLSCGSIPVNTLSIDYLLDKDFTAQTFAYYLPLMKLLVSNTSFQSAGAFFSGHI
                     YARKTLNDFLKKSLSLKTYSSDISLNELILKAKENIDSHISFYNDLNSYTR"
     gene            complement(2085..2396)
                     /locus_tag="PLU28_10130"
     CDS             complement(2085..2396)
                     /locus_tag="PLU28_10130"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOG35456.1"
                     /translation="MEVVRWEDAQVKIDKSDIKARKLFEYDASEFIVIKLSGFSELKK
                     HKTPVDALFFVIQGEGEFYVGSESLSASQGTVVYSPKNIEHGFKNISEKELQVLVVKL
                     K"
     gene            complement(2453..>2829)
                     /locus_tag="PLU28_10135"
     CDS             complement(2453..>2829)
                     /locus_tag="PLU28_10135"
                     /inference="COORDINATES: protein motif:HMM:NF024107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOG35457.1"
                     /translation="DWYIAGHSSGGRLACSYIKDRTEKISGLILLGAYPDSQNSLEGE
                     NIRVLSIYAGKDKMSPPLKVEKAKEYLPKETEYYFIPEGDGVQFSCSLQSFESSAGIG
                     MIQQQNEIFRQMLEFILEKDGM"
BASE COUNT          862 a          580 c          452 g          935 t
ORIGIN      
        1 taatgtcaag taccctgaag attttctcgt cacccatggc tgcctcccca aacggcactt
       61 tatccgctta ttcaaaataa gacttaagac tgtcaagctg ctccaacctg gctatgctta
      121 agccgtggca tacaagatcc ctgtacttct gaacatcatt aaaaaaatcc ttcagcccaa
      181 gaaatcttag ttcagcacct tcaaaagttt cagtaaacgg ctgtgttttt ttcatcacac
      241 atgtgaagct gtttattatt ttaggatttt caaaaatgct taaggtcaaa ggatacgcag
      301 aaaaaatctt ttttataaaa atatcaaact catcgttaaa cgctttattt accataaaaa
      361 gactgttaaa aaacgcctcc aatggagaaa aacttttatt gagactatag aagtgcgcat
      421 ttgccctttt ttcttttttt attataccaa gatcctcaag atcatttatc gcttttaaag
      481 tacttccagc accggttttc aatgagcgca taagctcgtt gacattctgc tcgctgttct
      541 caagcaagga gaaaattatc ttcatttcca tatttttata caatggatct atcatgaaca
      601 acctcctgac ggaacattaa attctttttt tggaatattt tattactatt ataataaaat
      661 actattactc ttgtcaagta tgacattttt tccccgaaat gtttttttgt tgtataatta
      721 aacttgaaaa ctgtcaatac aggaggtaaa aatgaaaaaa tatttattga tccttatggt
      781 ttttatattt tcttcagtgc tttatacaag acaaattgaa gtctggcata cttatggagg
      841 ctgcaaaaag gagtttttac aaaaaacatg tacttattac gcacaagaca acacacaggt
      901 cacgataaag cttgtaggct tttctgatta tgcttcaatg atgacagcac ttaatttatc
      961 cataaaaagc agcattccgc cggatgtcct gatatgtcca gacagtcttt ctaaaatact
     1021 tattgacagt ggagcagtgc tgaccgtaca aaaatttata gataccgata cagacttctc
     1081 tcaaaaaaac ctgtttccgt ttatacttga aaaattcaag tataaacaaa ccatacaggc
     1141 tttacccttc aatcctgtta ttttgatgag tttttacaat tcttcacttt caaaaaaact
     1201 tggattggac agcacatcta taccaagtac cttgagtgaa attcttcaga atgctaagag
     1261 cgctgctatg tcagaactgt acatccagga agatatttca tatgtaagca tgcctcttga
     1321 cagcattatg cttgaaaatt ttctgtgcat gacaaatctg ccttttaccg accatgataa
     1381 tggtagatcg gaaagccctg attcaattat gcccgacagc tcacagatat attctttctt
     1441 agacttctgg ctcagcatgc agaaccttgg atatgcaaag ctatacggct atgatagctg
     1501 gcaggaagcc atggactctt tcataaacgg gaaaacttgt ctgacctttt cctggtcaga
     1561 caacattaaa agcatcctga atcaatctga aaaaagaaag tttaaaactg ctctaggctt
     1621 tgtacctgtt tttaaaagaa tggaaggcgg tcctttggta agttgtgaag gtctttggct
     1681 ggtaagcggc catacccaag atgagattaa agagacatgg aactttttga agtttcttgt
     1741 ttccgaaaaa attcaggctg aatgtttttt atcctgtgga agcattcctg taaatacttt
     1801 gagcattgat taccttctag ataaagactt tactgcccaa acctttgcat actacctgcc
     1861 tttaatgaaa ctgcttgttt caaatacttc attccagagt gccggtgcat ttttttcagg
     1921 tcatatatat gcacgaaaaa cgctaaatga ttttttaaaa aaatctcttt cgcttaagac
     1981 gtacagctcg gatatttctt tgaacgagct gatcctaaag gcaaaagaga atattgacag
     2041 tcacatatct ttttataatg atcttaacag ttatacgaga taactcattt aagtttcaca
     2101 accaatacct gaagctcttt ttctgaaatg tttttaaatc catgctctat gtttttgggt
     2161 gaataaacga ctgttccctg ggatgcggat aaactttcac tacctacgta aaactcccct
     2221 tcaccctgta taacaaaaaa taaagcatcc acaggagttt tatgtttttt cagctctgaa
     2281 aatccactca gttttatgac tataaactcg cttgcatcat attcaaataa cttccttgct
     2341 tttatatcgc ttttgtcaat tttaacctgt gcatcttccc atctgacaac ttccatacta
     2401 tcacctccgt ttcaattata tccatacgga ggggttcttt ccgtaaccta tgttacatac
     2461 cgtctttttc cagtataaat tcaagcatct gcctaaaaat ttcattctgc tgttgtatca
     2521 ttccgatacc tgcactgctt tcaaaacttt ggagagaaca gctgaactgt acaccatcgc
     2581 cttcgggaat aaaataatac tctgtttcct ttggaagata ctctttggct ttttccacct
     2641 tcaggggagg agacattttg tctttccctg cataaataga aagcaccctg atgttctcac
     2701 cttcgagact gttctgagag tctggataag ctcccagaag aatcagtcct gatatttttt
     2761 cagttctgtc ttttatataa gaacatgcca gtctgcctcc agaggaatgt cctgctatgt
     2821 accagtctt
//
LOCUS       DAOSHR010000244         2813 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_34146_length_2813_cov_3.418419, whole genome shotgun sequence.
ACCESSION   DAOSHR010000244 DAOSHR010000000
VERSION     DAOSHR010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34146_length_2813_cov_3.418419"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="PLU28_10140"
     CDS             <1..502
                     /locus_tag="PLU28_10140"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOG35458.1"
                     /translation="CDYCGYKVKSVETHGLAQRGGTVSSHLRLGTNVYSPIIMSNSAD
                     MVVSLERNEAMRGMNDQLKDNGTLVYYDTSWQPLNVRLTKEKNVSTIDIQNIAEERGI
                     KVFRTFKKDIPDIRMQNIVLLALIAKEKLIPQLATEDYRSAMSDIMKDSTLELSMALF
                     DQIQKE"
     gene            complement(520..1935)
                     /locus_tag="PLU28_10145"
     CDS             complement(520..1935)
                     /locus_tag="PLU28_10145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35459.1"
                     /translation="MKRFSFFLSVLIISFFLISCSANIIPIEKTLKPNNITEYSAMAS
                     STSYEQAFAFTQAILVNAVSESVMLDFEDKYDKELYFSYPYITKELFLKEFQEYCSSQ
                     TVKYFEETDFSVIENKVYGQSIVLKTRLSLSQTKYDFIFYSLVCEYLVNKNLIFTGSR
                     LITEHSLNVSELISKKVKEEMSELNILEKSIIAKINIKDYYGAYSDLLSLKLAAADNP
                     NYDKYLSDIKAFFDQIQVDISYPQGILLDDETSAYIRVSAPEYTGLIGFDITGHNVEF
                     ASKKVFFADSSKDFTFRISKYSPEYSVTLSIGEIFRKSFTLTPDIHILRQRQIINKVF
                     FIPHSSSKTDTLPTDTLVTTPQISPPVEEVLIDDYYNFPKNDYTDLKVTGKPRQYVYI
                     FSYKNDKIYLIQNELINNSYTYLSNSSETLLRFKLEPQSNTLFIIFTFSAFKSTSDYS
                     LVEFRNLLFQNMADYIEIRIE"
     gene            complement(1993..>2813)
                     /locus_tag="PLU28_10150"
     CDS             complement(1993..>2813)
                     /locus_tag="PLU28_10150"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOG35460.1"
                     /translation="VIINMILDPLLIFTFKMGVLGAAWATFISVVIISLYSVYVMLKG
                     KYGLKMHIRDFKPDLKIIKKVIAIGLPPSLGNSVTSFAFSIIMAIVSGFGPSFISAYG
                     IFDRVNGFIAMFASGIGMAVTTMVGQLLGADMIKKAEDTVKKASTLTFTVVLVFSVLL
                     FFTGDTITRFFINDSEVIAIGKTIFMLVSFSNPFFSVMLIYMGAISGSGHTIQSTFID
                     VARLWAVRVPLIAFMASAYGINGIFYAMIISNIMALIISLLFYYLGNWRQKVIN"
BASE COUNT          959 a          456 c          504 g          894 t
ORIGIN      
        1 atgtgattac tgtggctata aagtaaaatc cgtcgaaacg cacggtcttg cccagcgggg
       61 aggtactgtc agttctcact tgcgtcttgg aacaaatgta tattctccta taataatgtc
      121 caactctgca gatatggttg tttctttgga acgtaatgaa gccatgagag gcatgaatga
      181 tcaactgaaa gataacggta cccttgtata ttacgataca tcatggcagc cgcttaatgt
      241 gaggttgact aaagaaaaaa atgtaagcac gatagatatt caaaacatag ctgaagaaag
      301 gggaattaaa gttttcagaa ctttcaagaa agatattccc gatataagaa tgcaaaatat
      361 agtactctta gcattgattg ctaaagaaaa actcattcca caactggcta cagaagatta
      421 tcgcagtgca atgagtgata taatgaaaga ttcaactctt gaattaagta tggcattatt
      481 cgatcaaatt cagaaagaat aatatttcta tcaagcgagt tattctatcc tgatttctat
      541 ataatctgcc atattttgga aaagtaagtt tctaaattcc accaatgagt aatcacttgt
      601 agatttaaaa gctgaaaaag tgaatattat aaaaagtgtg ttgctttgag gttcaagctt
      661 aaacctaaga agcgtttcag atgaatttga aagataagta tagctgttat ttatgagttc
      721 gttttgtata agataaattt tatcgttttt ataagaaaat atatagacat actgtctcgg
      781 ttttcctgtg actttaagat cggtatagtc attttttgga aaattgtaat aatcatcaat
      841 aagaacctct tccacaggtg gggaaatctg cggagtagtt accaatgtat ctgtaggaag
      901 agtatctgtt tttgatgatg agtgaggtat aaaaaaaacc ttatttatta tctgtctctg
      961 tctgagaatg tggatatcag gagtaagcgt gaaacttttt ctgaaaatct cccctatgct
     1021 taaagtaact gaatactctg gactgtactt tgaaatccta aaagtaaaat cttttgaaga
     1081 atctgcaaaa aatacttttt tgcttgcaaa ctcaacattg tgtcctgtaa tatcaaatcc
     1141 aatcaaacct gtatactctg gtgcgcttac tcttatatat gctgatgttt catcatcaag
     1201 cagtattccc tgaggatatg agatatcaac ttgaatctga tcaaaaaaag cttttatatc
     1261 agataaatac ttatcgtaat tgggattatc tgctgctgcc aatttcagtg aaagaaggtc
     1321 tgaataagca ccataatagt ccttgatatt tatttttgca ataatgcttt tctcaagtat
     1381 gttaagctca gacatttctt ctttaacttt ttttgagatg agttctgata cattcagact
     1441 atgctctgtg atcagtctac tgcctgtgaa aataagattt ttatttacga gatactcgca
     1501 aacaagagaa tagaatataa aatcatattt agtctgggaa agtgaaagtc ttgttttcaa
     1561 tacgatgctt tgaccatata ctttattttc tatgactgag aagtctgttt cttcaaaata
     1621 ctttactgtc tgggatgaac agtactcttg aaattctttc agaaaaagtt ctttggtaat
     1681 atatggatac gaaaagtaaa gctctttatc gtatttgtct tcaaaatcaa gcattacact
     1741 ttctgaaaca gcattcacaa gtattgcttg agtaaaggca aaggcttgtt cataggatgt
     1801 tgaagaagcc atagccgaat actctgtaat attatttggc tttagtgttt tttctatagg
     1861 aataatgttg gccgagcatg atataagaaa aaaagatatg attaaaactg atagaaaaaa
     1921 agaaaatctt ttcattgtac acctcagagc aggagttatt ttatataaat tataccataa
     1981 ctgttaagaa tatcagttta tgactttttg tcgccagttt ccaagataat aaaagagtag
     2041 ggaaataatc agagccatta tattactgat tatcatagca tagaaaatac cgtttatccc
     2101 ataagcactg gccataaaag ctataagcgg tactctaact gcccatagcc ttgccacatc
     2161 gataaaagtg gactgtatag tgtggcctga tccggaaata gctcccatat atataagcat
     2221 aaccgaaaaa aaaggatttg agaaagaaac aagcataaaa atggttttgc ctatggcaat
     2281 tacttctgaa tcatttataa aaaatcttgt tattgtatca ccggtaaaga ataggagcac
     2341 cgaaaagaca aggactactg taaaagtcag tgtagaggct ttttttactg tatcttcggc
     2401 tttttttatc atatctgccc cgagcagttg acctaccatg gtggtaaccg ccattcctat
     2461 tccgcttgca aacattgcaa taaaaccatt tactctgtcg aatattccgt aagcgcttat
     2521 gaaagatggt ccaaatcctg aaactatcgc catgattatt gaaaaagcga aggaagtaac
     2581 agagttgccc aaggaaggag gcaaccctat ggcgataacc ttttttatga ttttcaagtc
     2641 aggtttgaaa tcacgtatat gcatttttaa accgtacttt cctttaagca taacatatac
     2701 cgagtacaat gaaatgatga ctacagaaat aaaagttgcc caagctgccc ctaggacgcc
     2761 catcttgaaa gtaaatatca gaagcggatc aagaatcatg tttataataa ctg
//
LOCUS       DAOSHR010000245         2807 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_34249_length_2807_cov_4.218387, whole genome shotgun sequence.
ACCESSION   DAOSHR010000245 DAOSHR010000000
VERSION     DAOSHR010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34249_length_2807_cov_4.218387"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..1157)
                     /locus_tag="PLU28_10155"
     CDS             complement(63..1157)
                     /locus_tag="PLU28_10155"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HOG35461.1"
                     /translation="MESYVYPIKIAFLTFPLYAFFLTIPFMLYQYRKHRYINKLRILG
                     FYSFVLYFITAYYLIILPLPKLSDMPLTHSADVRYYQIVPFSFVRDIIRESGIIFSKP
                     ETYLGVLTQRSFLQAFFNGILLFPLGVYLGYYFKKPFLKTVLLAFGVSLFFEITQLTG
                     LYGIYKTPYRLFDVDDLFLNTLGGALGWIISPVFSYFLPDMRKIDENIDLKKMQVSFV
                     RRSVAVGIDWFLIMLVTSVLGFSENFFFFVLTVFLYFIVLVYLTNGKTIGKSLVKIRV
                     KGAKARLSFEEVFKRYGLLYLFFFGIDYILSLMIKENNDPFISFFLIFCSLIYNLFLI
                     INIFMHALKKDKLLFYEKTSHTRNVIDDKK"
     gene            complement(1171..1398)
                     /locus_tag="PLU28_10160"
     CDS             complement(1171..1398)
                     /locus_tag="PLU28_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35462.1"
                     /translation="MLVMELISNLLFIVSMILILGSWYFFSRSSKEAKKGIEKDADKI
                     KKYDKQGFIWIGMCILVVLLSYFFAYLARKI"
     gene            1479..>2807
                     /gene="dnaB"
                     /locus_tag="PLU28_10165"
     CDS             1479..>2807
                     /gene="dnaB"
                     /locus_tag="PLU28_10165"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOG35463.1"
                     /translation="MQNISLTPPSSIEAEESVLGSIFLDPLILPEIIEEIRWEDFYYD
                     KNKTIFKSMEELFEKGEPVDVVTVIENLRANGKLASIGGEEFLISLAQATPTTANFLY
                     YTKIVKEKALLRTLITASSEIVESVRTVGDAQEILEFAEKRIFSIAEARATRTYDTLP
                     NIMHGVFEKIEEFKKRAQEGSNALVTGISTGYKSLDRITSGFHKSDLVIIAARPSMGK
                     SSFALNLAANVAVREKKSVAIFSLEMSKEQLANRVLCSEASVDLQKVRTGMISEDEWN
                     KLVYHAGELSKSKIIFDDEPGLDPRTLRAKARRMKREFAIDAVFIDYIQLMTSRVRDN
                     SENRQQEISEISRSLKLLARELDITVIALSQLSRAVEQREDKRPRLSDLRESGAIEQD
                     ADMVMFLYRDAYYKRKKEDSQNDLILNQAHEAELIIGKQRNGPIGTLSMVF"
BASE COUNT         1034 a          500 c          499 g          774 t
ORIGIN      
        1 tgctgggtta cacacctaat ccagactctc ctcctaacag tccgccggtt aacgccgcct
       61 gattattttt tgtcatcaat tacgttacgc gtatggcttg ttttttcata aaaaagaagc
      121 ttgtcctttt ttaaagcatg cataaagata tttataataa gaaaaagatt ataaatcaaa
      181 ctgcaaaata tcagaaaaaa agatataaaa ggatcgttat tttcttttat catcaaagat
      241 aatatgtaat ctatcccaaa gaaaaaaaga tatagaagac cgtatctctt aaaaacctct
      301 tcaaaagaaa gccttgcctt tgctcccttt actctgatct ttactaatga ctttcctatg
      361 gttttaccgt ttgtcaggta aactaaaact ataaaataaa gaaaaacagt cagcacaaaa
      421 aagaaaaagt tttcggaaaa accgagcact gaggtaacta acatgattaa aaaccagtct
      481 ataccgactg ctaccgacct ccttacaaaa gaaacctgca tctttttaag gtcaatgttt
      541 tcatctatct ttctcatatc gggaagaaaa tatgaaaaaa caggtgatat aatccagcct
      601 aaagctcctc ccagagtatt taaaaaaagg tcatccacat caaagagcct gtaaggagtt
      661 ttgtagattc cataaagtcc tgtcagttgt gtaatctcaa aaaaaagaga aactccaaag
      721 gctaaaagaa ctgtttttaa aaagggcttt ttaaaataat atcctaagta tactcccaag
      781 ggaaacagaa gaataccgtt gaaaaatgcc tgtagaaatg acctctgtgt cagtactcca
      841 agatatgttt ccggcttgga aaaaattata cccgactccc ttataatatc tctcacaaag
      901 ctaaacggta ctatctgata gtacctaaca tcagccgaat gggtgagagg catatccgaa
      961 agcttgggca aaggaagtat tataaggtaa tatgctgtga taaaataaag gacaaaagaa
     1021 taaaacccaa gtattctcag tttatttata tacctgtgct ttctgtactg ataaagcata
     1081 aacggaatag taaggaagaa agcatataaa ggaaatgtga gaaaggctat ttttatagga
     1141 tacacataac tttccatact tcacctttcc ctatattttc cttgccaaat aagcaaaaaa
     1201 atatgaaaga agaaccacca atatacacat tcctatccat ataaagcctt gtttgtcata
     1261 tttttttatt ttatcggcat ccttttctat ccctttttta gcttcttttg aagaacgcga
     1321 gaaaaaatac catgatccga gaataagtat catcgatact ataaaaagaa ggtttgaaat
     1381 aagttccata acaagcatag attccacctc aaaagtattc ttttttatca tgctataata
     1441 tatatgtgta tcaattatac cagaatatgg aggcacgaat gcaaaacatc tcattaacac
     1501 caccttcaag catagaagca gaagagtcgg tacttggcag tatattcctt gatccgctaa
     1561 tattaccgga aataattgaa gaaatacgat gggaagactt ttattacgat aaaaataaaa
     1621 ctattttcaa atctatggag gaactgtttg aaaaaggaga accggtcgat gttgttaccg
     1681 taatagaaaa tcttcgagca aatggaaaac ttgcttcaat aggaggagaa gagtttctta
     1741 taagccttgc acaggctact cctactacag ctaactttct ttactataca aaaattgtaa
     1801 aagaaaaagc tcttttaaga actcttatca ccgcttcgtc cgagatagta gaatctgtaa
     1861 gaaccgtagg agatgcccag gaaattcttg agttcgctga aaaacgtata ttctctatcg
     1921 ctgaagcacg agcaaccaga acttacgaca ctttgccaaa tataatgcat ggagtctttg
     1981 aaaaaataga agagtttaaa aaaagagctc aggaaggttc aaatgcacta gtaacaggaa
     2041 tatccacagg atataaatcc cttgacagaa ttacttcggg gtttcataaa tctgatctgg
     2101 taataattgc agcaagacct tcaatgggaa agtcttcttt tgcgcttaac ttggctgcta
     2161 atgttgcagt aagagaaaag aagtccgttg cgatattcag tcttgaaatg tcaaaagaac
     2221 agcttgccaa cagggtttta tgctcagaag cttctgttga ccttcaaaag gtacgtacag
     2281 gaatgatctc agaagatgaa tggaataaat tggtgtatca tgccggtgaa ctgtcaaaat
     2341 caaaaataat atttgatgat gaacccggac tagaccctag aactctaaga gcaaaagcaa
     2401 ggagaatgaa aagagagttt gcgatagatg cagtattcat agattatata cagcttatga
     2461 catcaagagt aagagataac agcgaaaaca ggcagcagga gatatcggaa atatccagat
     2521 cccttaagct tcttgcaaga gagcttgata taactgtaat agccctttca cagctctcaa
     2581 gagcagttga acagagagaa gataaacgtc cacgcttaag tgacttgaga gagtccggag
     2641 ccattgaaca ggatgcagat atggtaatgt ttctgtatcg tgatgcttac tacaaaagaa
     2701 aaaaagagga cagtcaaaat gatcttattc ttaatcaagc tcatgaagca gaactaatca
     2761 taggaaaaca aaggaacggt ccgataggaa cattgagtat ggttttt
//
LOCUS       DAOSHR010000246         2791 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_34521_length_2791_cov_5.192982, whole genome shotgun sequence.
ACCESSION   DAOSHR010000246 DAOSHR010000000
VERSION     DAOSHR010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34521_length_2791_cov_5.192982"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1949
                     /gene="polC"
                     /locus_tag="PLU28_10170"
     CDS             <1..1949
                     /gene="polC"
                     /locus_tag="PLU28_10170"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF019353.2"
                     /note="catalyzes DNA-template-directed extension of the
                     3'- end of a DNA strand by one nucleotide at a time;
                     required for leading strand synthesis; PolC exhibits 3' to
                     5' exonuclease activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HOG35464.1"
                     /translation="MDIFDDEKKAYLTTITNSRLISSMTEKFSLFPDKVCAPQMGNTD
                     SFIIDVMNCAREVYGTVLHPLIEKRLSDEVQAIIKSGYAVMFTLSQKIVAHSEQAGYP
                     VVSRGTAASSLVAFVMGITGINPLKPHYYCKECSYVEFRNDCGSGYDLTDKKCPYCGE
                     LMKKDGHNIRFEIFLGGDGKKPPDIDLNFSGEYQSQVHRYLTELFGRDRCFKAGTINA
                     SSYRLARRMAEKYQSVKPFNSAEKAWIVHNLMNVRITTGQHPGGMVIIPSSMQAEDFT
                     PVQYPSDDQSKELLTTHFDYEAIEKQLLKVDALGHDGPTFLKRLTELTRYNHEDIPMN
                     DAKVIKMFSSPLPMNISLDEIGIKTGTVGIPEVWTPYAQKMLLETRPQNFHDLIRTAG
                     LSHGSNIWFNNAKELIMRDKKLKEDVISCRDDILEYLEKFSVPRDIILSIIQKVKKGS
                     GVDEQTKRVLRDKNVPQWYINSCDRIRYLFPKAHSVSYMVTSYKIAFYKLYFPLEFYS
                     VYFSIHAWNFDVMNIKSSVKTLFSSMENKENKGQYYYYRFGEVQTAYEMIKRGYSFLK
                     PDIYTSAGFDFIINGNSLRVPLVKIGKLGPKSIAKILEQRKIKFTSKEDFINRTKLSA
                     SVTELLEQNGVLNFPRNSVKLKLF"
     gene            1972..>2791
                     /locus_tag="PLU28_10175"
     CDS             1972..>2791
                     /locus_tag="PLU28_10175"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HOG35465.1"
                     /translation="MKILTASQAKDMDLQTIKKGISEDTLIEQAAYSVFEVVAELRPL
                     KVLCVAGAGNNGADSICTARILANNSIYADILPAAPGKNKSSGYIKQMEIAQNFNVNI
                     VEDKDTDYDSYDIIVDGITGTGFKGPMDTETAAIADKINNSRATVIAIDIPTGIDSDT
                     ALADEHAIFADITVTFSAVKIGHLLYPGCRHCGRIKVADISFDKSYFQNIMRHLVTAD
                     TAKALLPRRYGDSNKYSYGRVLVLASSRLYPGSGILNAIGAFRSGAGLVKLITPS"
BASE COUNT          947 a          553 c          572 g          719 t
ORIGIN      
        1 ctatggatat attcgatgat gaaaaaaaag cttaccttac tactataaca aactcccgtc
       61 tcataagcag catgactgaa aaattcagcc ttttcccaga taaagtttgc gctcctcaga
      121 tgggaaatac agatagtttt ataatagatg taatgaactg tgccagagaa gtatacggaa
      181 ctgttctgca ccctttgatt gaaaaaaggc tttcagatga agtacaggcc ataataaaaa
      241 gcgggtatgc cgttatgttt actttatctc agaagatagt agcccattca gaacaggccg
      301 gctatccggt agtatcaaga ggaacagcgg catcatcgct tgtggctttt gttatgggaa
      361 taacgggaat aaacccatta aaacctcatt actactgcaa agaatgctca tacgttgaat
      421 ttcgtaatga ttgcggtagc ggatatgacc tgacagataa aaaatgcccg tactgcgggg
      481 aactgatgaa aaaagacggc cacaatataa gatttgaaat atttcttgga ggtgacggaa
      541 aaaaacctcc cgatatagac cttaacttct ctggagagta tcagtcgcag gtacaccgtt
      601 accttacgga gcttttcgga cgtgacagat gctttaaagc cggtacgata aatgcttcat
      661 cataccgtct ggcaagaaga atggctgaaa aataccaatc cgtaaagccc ttcaactctg
      721 cagaaaaagc atggatagtc cacaatctta tgaatgtcag gataacaacg gggcagcatc
      781 ccggaggtat ggttattatt ccttcttcta tgcaggcaga agattttaca cctgttcagt
      841 atccttctga cgatcagtcg aaagaacttc taacaacaca ctttgattat gaagccattg
      901 aaaaacagct tctcaaagtt gatgcccttg gacatgacgg cccgactttt ttaaagaggc
      961 ttacagagtt aacaagatac aatcatgaag atatcccgat gaatgatgca aaggttataa
     1021 aaatgttctc atctcctcta ccaatgaata taagtctgga tgaaatagga ataaaaaccg
     1081 ggactgtcgg tattccggaa gtctggacgc cttatgctca gaagatgttg cttgaaaccc
     1141 gtccacaaaa ttttcatgac ctcataagaa ccgcaggact ttcgcacgga agcaatatat
     1201 ggtttaacaa tgccaaagaa cttattatga gagataaaaa attgaaggaa gatgttatat
     1261 catgccgtga cgacatacta gagtatctgg aaaaattttc tgtaccaagg gatataatac
     1321 tctcaataat acagaaggta aaaaaaggca gtggagttga tgaacagacc aaaagagttc
     1381 tcagggataa aaatgttcca caatggtata taaattcatg tgaccgtata agatatcttt
     1441 ttcccaaagc acacagtgtt tcatatatgg tgacctctta taaaatagcc ttctataagc
     1501 tctactttcc tttggagttt tacagcgtct acttttcaat ccacgcatgg aactttgatg
     1561 tcatgaatat aaaatcctct gtaaaaactc ttttctcctc aatggaaaac aaagaaaaca
     1621 agggacagta ctattactac agatttggag aagtccagac agcctatgaa atgatcaaac
     1681 gcggatacag ttttctaaaa cctgacatat atacctccgc aggatttgat tttatcataa
     1741 acggtaacag cctgcgggta cctctggtaa aaatcgggaa actcggaccc aagagtatag
     1801 ccaaaatttt ggaacaacga aagatcaagt tcacatcaaa agaagatttt ataaacagaa
     1861 ccaaactttc cgcctcagtg acagaacttc tggaacagaa cggggtttta aattttccgc
     1921 gaaacagtgt aaaattgaag ctgttctgat aaaaaaacgg aggtgaaaat aatgaagatc
     1981 ctcaccgctt ctcaggcaaa agatatggat ctgcaaacaa taaaaaaagg catttcagag
     2041 gacacactca tagaacaggc tgcatattcg gtttttgaag tagtagcgga actcagacca
     2101 cttaaggtgc tttgcgttgc gggagcagga aataacggtg ctgacagcat ctgcacagca
     2161 cggatccttg caaacaactc aatatatgca gatattcttc cagctgcacc cggaaaaaac
     2221 aagagctccg gatacattaa acagatggaa atagctcaaa atttcaatgt aaatatagta
     2281 gaagataaag ataccgatta tgacagttac gatataatag ttgacggaat caccggaaca
     2341 ggttttaaag gccctatgga tactgaaact gccgctattg cagataaaat taacaactct
     2401 cgtgccacgg tgattgcaat agatatccct acaggaattg actcggatac ggcacttgcc
     2461 gatgaacacg ccatctttgc agatattacg gtaacttttt cagcggtaaa gataggacat
     2521 ttgctctacc cgggatgcag acactgtgga agaataaaag ttgcggatat ttcctttgac
     2581 aagagctatt ttcaaaacat tatgcggcat ctggttacag ccgataccgc aaaagctctt
     2641 ttgccaagaa gatatggcga ttcaaataag tacagctatg gcagggttct tgtgcttgca
     2701 tcaagccgtt tatatccggg ttccgggata ctcaatgcca taggagcttt cagaagcgga
     2761 gccggactgg taaaactcat aactccatcg g
//
LOCUS       DAOSHR010000247         2785 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_34626_length_2785_cov_6.395604, whole genome shotgun sequence.
ACCESSION   DAOSHR010000247 DAOSHR010000000
VERSION     DAOSHR010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34626_length_2785_cov_6.395604"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="PLU28_10180"
     CDS             <1..651
                     /locus_tag="PLU28_10180"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOG35466.1"
                     /translation="SIGNHEFYKYYGHGPNTEAEEKELINNFLKFTGKEKVYSDCWIK
                     GYHFISLGSEKTYTFDQPSTVQAYLSESQLKWFEEKINEKYEKNKYIFVIFHLPPDDT
                     VKFSHYYKENIKQEERFLKIIQRYPNIVYLTGHSHISPLVYDNIFYKSPYGYLMIDSS
                     SVSRPVILDESTGEEKNLPGGRSFGLYFQIYDESMEIRVRDFSKHEWIGESHRYNK"
     gene            complement(669..1655)
                     /locus_tag="PLU28_10185"
     CDS             complement(669..1655)
                     /locus_tag="PLU28_10185"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOG35467.1"
                     /translation="MLTTGGILTLLYFLGFLVLIKIAPFTMTAIVIGLYFSILVRVPE
                     KFLVQKFKLNATLSRIISNIFFIGIVLYAFGNLIPIIIDQGKVVLENLKITSSGDLYE
                     AMPEWIVKLIEDFSHKLSSTGLSILDKVVGSAPNFIASIVIAIITAATINGIISKVSD
                     KFWILFPSSERSKGVLFLKKTFRDFEVFVQGQFVVATFVGLIVGFSSFVAGIKGAVFL
                     GVLSWITDFIPYLGIVIAAVPMLLIGYATRGLTGLIIGIVIIVAANQLEVWFFSPKVQ
                     SSNLKIHWYVIFIIIFIFGELFGVIGTLLALPCLIFMINYWNTFVAPELKKK"
     gene            1763..2347
                     /locus_tag="PLU28_10190"
     CDS             1763..2347
                     /locus_tag="PLU28_10190"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HOG35468.1"
                     /translation="MDKKTLRSIVLNQRINTDAEQILEKSSRICANLLKVLSELNYET
                     IALYSSFRKEVDLSEVFHSIRKDKKVVFPFISDKKMIFRRADSEKDFRLGKFGIMEPM
                     GEEEKKIDLFILPAVAFDRNFYRLGYGGGYYDRYFSENLKTVLIGSVFDFQIVQDVFH
                     EEHDVQADMIVSESGVLRRKKAEKKDEIKGGESR"
     gene            2344..>2785
                     /locus_tag="PLU28_10195"
     CDS             2344..>2785
                     /locus_tag="PLU28_10195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis isomerase"
                     /protein_id="HOG35469.1"
                     /translation="MRKYPFFYMNSFSCVPGYGNPAAVVYGAENISETDMQKIAKING
                     LSETAFIINEKTSGYDYRIKFFTPEKPINMCGHASIAAAYAYSEMNDMPCEISLTQKT
                     DVGTLKIKVERLNEYSRVSLQMDTPDIERTFHGQYKEFAQALGLK"
BASE COUNT         1008 a          439 c          537 g          801 t
ORIGIN      
        1 tctatcggaa accatgaatt ctataagtat tatggacatg gtcccaacac tgaagcagaa
       61 gaaaaggaac ttataaataa ttttttgaag tttacaggaa aagaaaaagt ttattctgat
      121 tgttggataa aaggatatca tttcataagt ttgggatcag aaaaaactta cacctttgat
      181 cagcccagta ctgttcaggc atatctttcc gagtcacaac tgaaatggtt tgaagagaag
      241 ataaacgaaa agtatgaaaa gaacaaatat atttttgtta tttttcatct gccgccggat
      301 gatacggtta agttttcaca ctactataaa gagaatataa agcaggaaga aagattttta
      361 aagattatcc aaaggtaccc taatattgta tatcttaccg ggcattccca tataagtccg
      421 ctggtttatg acaatatttt ttataagtcg ccttatggat atctcatgat agactcttca
      481 tcggtatcgc gtcctgtgat tcttgacgag agtacgggcg aagaaaaaaa tcttcctggt
      541 ggaagaagct ttgggttgta ctttcagatc tatgacgaaa gtatggaaat aagggtgcgg
      601 gatttttcaa aacatgagtg gataggcgaa agccacagat acaataaata aaaaagtaag
      661 gctgtgcctt actttttttt gagttccgga gcaacaaagg tattccagta atttatcatg
      721 aatattaagc atggaagtgc cagcagagtc ccaattactc caaaaagctc tccgaaaata
      781 aagattataa taaaaataac gtaccagtgt attttcagat tggaactctg aacttttgga
      841 ctgaaaaacc aaacctcaag ctggttggca gcgacaatta ttactatacc tattataaga
      901 cctgttagtc ctctggtagc atagcctatg agaagcatag gtaccgcagc aataactata
      961 ccaagataag gtataaaatc tgttatccag gaaaggacac ctaaaaacac agctcctttt
     1021 atgcctgcta caaacgatga aaaacctaca attaacccta caaaagttgc aactacaaac
     1081 tgtccctgta caaacacttc aaaatcccga aatgtttttt ttaggaaaag aacccctttt
     1141 gatctttcag agcttgggaa caatatccaa aacttatcag atactttact tattataccg
     1201 tttatagtag ctgcggttat tatagcaatt acgattgaag caatgaaatt aggagctgag
     1261 cctactacct tatcaagaat tgaaagacct gttgaagaaa gtttatgcga aaaatcttct
     1321 ataagcttga ctatccattc tggcatggct tcatagagat cgccggaaga agtgatttta
     1381 agattttcaa gaacaacttt tccctggtca ataattatcg gaataagatt tccaaatgca
     1441 taaagaacta tcccaataaa aaatatgttt gagattattc tgcttaatgt ggcattaagc
     1501 ttaaattttt gcacaagaaa cttttcaggt actcttacca gaatagaaaa ataaagtcct
     1561 ataactatag cagtcatagt aaatggagct atttttatca gaacaaggaa tccaagaaaa
     1621 taaagaagag taagtatacc gccagtagta agcatttatt acctccattt tttatagtat
     1681 aggaagtact ttggtataat ttatgttatc ataaattata taggaagata attaactgaa
     1741 gattaagata agagaggcgt atatggataa aaaaactttg agaagtattg ttttgaatca
     1801 gagaattaat actgatgctg aacagatatt ggaaaaaagc agccgtatct gtgctaatct
     1861 gcttaaagtt ctatcggagc ttaactatga gactattgca ctttatagtt ccttcaggaa
     1921 agaggttgat ctgtcggagg tcttccattc tatcagaaaa gataaaaaag tagttttccc
     1981 ttttatatca gataaaaaaa tgattttcag aagagcagac tctgaaaaag atttcaggct
     2041 gggaaagttt ggtattatgg aaccgatggg cgaagaagag aaaaaaatag atctgttcat
     2101 tcttcctgct gttgcatttg acagaaattt ttacagactg ggatacggcg gagggtacta
     2161 tgacaggtat ttttctgaaa atttaaagac agtactgata ggtagcgtat ttgattttca
     2221 gattgttcag gatgtttttc atgaagaaca tgatgttcaa gccgatatga tcgtaagcga
     2281 aagtggtgtt ttaagaagaa agaaagcaga aaaaaaagat gaaattaaag gaggagagag
     2341 ccggtgagaa aatatccgtt tttttatatg aactcatttt cttgtgttcc ggggtatggc
     2401 aatcctgcag cagttgtgta cggagcagaa aatatcagtg aaacagatat gcagaaaatt
     2461 gcaaaaatca acggactttc agaaacagca tttataataa acgaaaaaac tagcggttac
     2521 gactacagaa taaaattttt tacacctgaa aaacccataa acatgtgcgg acatgcaagc
     2581 attgccgcgg catatgcgta ctcagaaatg aatgatatgc catgtgagat atctctaacc
     2641 cagaaaaccg atgtgggaac attaaaaata aaagttgaaa ggctaaacga atattcaaga
     2701 gtatctcttc aaatggatac tccagatatt gaaagaacat tccacggtca atataaagag
     2761 tttgcacagg cacttggact gaagg
//
LOCUS       DAOSHR010000248         2783 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_34663_length_2783_cov_5.649927, whole genome shotgun sequence.
ACCESSION   DAOSHR010000248 DAOSHR010000000
VERSION     DAOSHR010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34663_length_2783_cov_5.649927"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            319..2139
                     /locus_tag="PLU28_10200"
     CDS             319..2139
                     /locus_tag="PLU28_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35470.1"
                     /translation="MNKKYAVLLITLSIVGLLLLNSCAGDLSGGLSVELTMSSDSKSP
                     VLITTDSSITFFIKLNQPISSVGSAQLVINPISIANSSEILLNTSRVDARTFKVSFQT
                     DVPGNYSFSAKFIQDGNKKIFTSKNTIEKYIEAGTVNGPKIKEITLKNVDARIIQEQD
                     STRVITAVVDPSQTPKVLFTVEATGMNFLTLGIFGENMNSPLNITKPEDMNIDLNIFS
                     KEGIYNYTVMLYDGQIGETLKIFDTKKIKFIVTNDKIPPTIDTGDYKVINDSTINIES
                     FSDTVPLRFGFKEPPSDGNIGLYKVKIDVDGKTAYEKTFPNGVSEDYPVVNLGFSKSV
                     YSVNLYAEDLANKTTTKAYNINLKKIDIKASLIAKKVSLDQEIQASEQLEYGERIKFD
                     CSVASDTVDLTDGATFTFVIKDDSNEGKEIFNMKQVNQRSVTYSNFMPPEGKNYFSLH
                     TVVEKGGIKYYVTDQKVLNVQDVVAPELYKAVVTIGSTQTVIFDKDNPLNEKPIKESE
                     IGFNPPLLTVFFRDRSKISYKPTVRVSIDDVPMSNFSIDTFDANLYRYVSYRGSISKT
                     LVKQTYTFSIAGDAIEDSYGNFLCDVNKNPMPYKFSLTVE"
     gene            2153..>2783
                     /locus_tag="PLU28_10205"
     CDS             2153..>2783
                     /locus_tag="PLU28_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35471.1"
                     /translation="MRRRHLFSLIIILIVVLSGCIFFQDTASPVFLNTPSIKNFSKIT
                     KANDDIIFDLHIQDNSGINYVSVYADGIPTPIATPIGDGTPYSKKEFNGEISVKAPYI
                     SNSYGLTVKVYDVTGNPPAVKTLDRKLYTPDRTEPSVGTPVLYPLPARGSGYFELVSS
                     VSDIGSGISRVRFNIDDKIIKDLKVVRQADGLDDYVLIQEETASWMLASA"
BASE COUNT          911 a          529 c          558 g          785 t
ORIGIN      
        1 gcagcaaaca taccaaaagg ctgcaggttt cacacccgct gtccctacgc taaacaaagc
       61 tgtatagaca atcctgaacc ggaacttgtt gaagttggtc caaacggtca tctcgcagca
      121 tgttctcgat acatcgatat caaaacggaa caccctgtac tataatagct gctccctaac
      181 cgggagcagt ttttttgttg ttaagcaatc tcttttttcc ggacgatatt attaatagct
      241 gttttataca ttaaaggatt ttgagtatta tatatagaag aaatatatga aaagaagatt
      301 ttgattggag gggttgaagt gaataaaaaa tatgcggtac ttttaataac tttatctatt
      361 gtaggacttt tgctcttaaa ttcatgtgca ggagacctct ccggtgggct ttcggttgag
      421 cttaccatgt cttccgattc caaaagccct gttttgataa ctacagactc atctataact
      481 ttctttataa agctaaacca gccgatatct tcggtcggta gtgctcagct tgtaataaac
      541 cccataagca ttgccaactc atcagaaatt cttctaaaca cctccagagt tgatgcacga
      601 acattcaagg tttcttttca gactgatgtt ccgggtaatt actcttttag tgccaagttt
      661 atacaggacg gaaataaaaa aatctttact tccaaaaata caatagaaaa gtatatagaa
      721 gcaggaactg taaacggacc gaaaataaaa gaaataactc ttaaaaatgt tgatgcaagg
      781 ataatacagg aacaggacag cacaagagtc attactgcgg tagtagatcc aagccagaca
      841 cctaaggttc tttttactgt ggaagctaca gggatgaact ttcttactct tggaatcttt
      901 ggggaaaaca tgaactctcc tctgaatatt accaagccgg aggatatgaa tattgacctt
      961 aatattttca gcaaagaggg aatatacaac tatacagtaa tgctttatga cggtcagata
     1021 ggtgaaactt tgaagatatt tgataccaaa aagattaagt tcatagttac aaacgacaag
     1081 attcctccta caatagatac tggggactat aaggtcataa acgactccac cataaacata
     1141 gagagctttt ccgatacggt gcctttaagg tttggattta aagaaccgcc ttcggacgga
     1201 aatataggac tatacaaggt aaaaatagat gtagatggta aaactgcata tgaaaagacc
     1261 tttcccaacg gcgtatctga agattatccg gttgtaaacc ttggtttttc aaagagtgtt
     1321 tacagtgtca acctatatgc cgaggatctt gccaacaaaa ctaccaccaa ggcgtataac
     1381 ataaatctga aaaaaataga tataaaagca tctctcatcg ccaaaaaggt ttctcttgac
     1441 caggagattc aggcatcaga gcagttggag tacggagagc gcataaagtt cgactgttct
     1501 gtggcaagtg ataccgtaga ccttaccgat ggtgctacct ttacttttgt tataaaggat
     1561 gacagcaacg aaggcaaaga gatcttcaat atgaaacagg ttaatcagag aagcgttaca
     1621 tactctaatt tcatgcctcc tgaaggaaag aattactttt cacttcatac ggttgttgaa
     1681 aaaggcggca taaagtacta tgtgaccgat caaaaagttc ttaatgttca ggatgttgtt
     1741 gctccggaac tttataaggc ggttgttacc ataggctcta ctcagaccgt aatctttgat
     1801 aaggataatc ctctcaatga gaaaccgata aaagagtcgg agataggttt taacccgccg
     1861 cttctgactg tgtttttcag agacaggtca aagataagtt ataaacccac agtaagagtc
     1921 agcatagatg atgttcccat gtcaaacttt tctatagata ctttcgatgc taatctttat
     1981 agatatgttt cttacagagg gagtataagc aagacacttg ttaaacaaac ctacacgttc
     2041 agtatagcag gagatgcaat agaagacagt tacggcaact tcctttgtga tgtaaacaag
     2101 aaccccatgc cttataaatt ctctctaacc gttgagtaaa gagggtggaa aaatgcgtag
     2161 aagacatctt ttttcattaa taataatatt aattgtggtt ttatcgggct gtatcttttt
     2221 tcaggataca gcttctcccg tttttttaaa tacccccagt ataaaaaact tctcaaagat
     2281 aaccaaggcc aacgacgata tcatctttga ccttcacatt caggataatt caggaataaa
     2341 ttatgtatcc gtatatgctg acggaatacc gaccccaatc gccacgccta taggagatgg
     2401 tactccatac agcaaaaagg agtttaatgg agaaatatcg gttaaagctc cttatataag
     2461 caactcgtat ggtcttacgg taaaagtgta cgatgtaact ggcaaccctc cggctgtaaa
     2521 gactctggac agaaagcttt atactcctga tagaaccgaa ccttcagtgg gtacacctgt
     2581 tctatatcct ctgccggcaa gaggcagcgg gtactttgag ctggtatctt cggtaagcga
     2641 tataggttca ggaatatcgc gggtaagatt taacattgat gataaaataa taaaagatct
     2701 gaaagtcgtc agacaggcag acgggcttga tgattatgtg ctgattcagg aggaaacggc
     2761 ttcttggatg cttgccagtg caa
//
LOCUS       DAOSHR010000249         2758 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_35135_length_2758_cov_5.635960, whole genome shotgun sequence.
ACCESSION   DAOSHR010000249 DAOSHR010000000
VERSION     DAOSHR010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35135_length_2758_cov_5.635960"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="PLU28_10210"
     CDS             complement(<1..382)
                     /locus_tag="PLU28_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35472.1"
                     /translation="MNDSVWAKKIISAQNSDGSWGFFHTMCSISDSKKITTEQALRRL
                     KNLGFTKEDKVISKALSYIQKCLEGTVAIPDRREKFRDWDVFFSFIFSAWLKLFDAQT
                     KQSEKICLKLGSVIEKAFGKKVFNI"
     gene            complement(493..1716)
                     /locus_tag="PLU28_10215"
     CDS             complement(493..1716)
                     /locus_tag="PLU28_10215"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HOG35473.1"
                     /translation="MKILVINCGSSSIKYQLLDMDTENVIAKGLVERIGIDGSRIVHS
                     VNGKKTTLNDDIKDHEKGLKLVLDILVSKDLGVLNSLDEIDAIGHRVVHGGEKFASSV
                     RINSEMLNTMEMLSFLAPLHNPANITGIKAAMRILPNVPQIGVFDTAFHQTMPEKSFM
                     YAIPKEYYDKYKVRRYGFHGTSHRYVSQRAAKFLGKDVRTLKIITVHVGNGGSVAAVC
                     NGRSVDTSMGFTPLEGLVMGTRCGDIDPSIVTFLQQEEGLSSKQVNDILNKKSGMAGL
                     TNGLSDMRDIENGAMNGDAICTLAHDIYEYRIAKYIGAYTAAMNGVDVIVFTAGVGEN
                     SPLLRKNIVTKYLGYLGITLDEQKNNMRGDDNEISTKDSKVKVLVIPTNEELMIARDT
                     KEIVEKNLNELKTTL"
     gene            1940..>2758
                     /locus_tag="PLU28_10220"
     CDS             1940..>2758
                     /locus_tag="PLU28_10220"
                     /inference="COORDINATES: protein motif:HMM:NF024573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOG35474.1"
                     /translation="MLVSLSLKNFGLFKDENIEFSDGLNIISGESGAGKSMFISAINS
                     LITGQFPSELKNQEGMISAFFSVNDTVKKIIFQATGIESNELVISANFSSKKTLYRIN
                     GMVVTKNIIEEIGKYLIEIHSQESSAILRDPLYQNKLMYDIIINKHREAFTEYNSLYS
                     EYSMLKEKAAAIPSDPSEVLRKIDTLNFQINEIKKTDPQPNEDTELSERFRALNNVQE
                     IKNNLYENMQLLKDSEEFNTDNSIGTVAYNLSKIREYGFKDTEQFAISIQQQLLE"
BASE COUNT          860 a          538 c          476 g          884 t
ORIGIN      
        1 caatattaaa aacttttttt ccaaaagctt tttcaataac gctgcccagt tttagacata
       61 ttttttccga ctgtttagtc tgtgcatcaa atagctttaa ccatgctgaa aaaataaaag
      121 aaaagaatac atcccagtct ctgaactttt ctcttctgtc aggtattgca acagttcctt
      181 cgagacattt ctgtatatat gataaggctt ttgaaataac cttatcttct ttagtaaatc
      241 caagattttt gagtcttctc agagcttgtt cggttgttat tttttttgag tctgaaatac
      301 tgcacattgt atgaaaaaat ccccagcttc cgtctgaatt ttgtgcagat attatttttt
      361 ttgcccatac agaatcattc atcttaacct cctgtcccct aaaacactgt aaaataatct
      421 atatgttcag aaaattatat caaatatata aaaaaaacac gaagcggttt aagcttcgtg
      481 ttttgaatag agttataaag ttgttttcag ttcgttgagg ttcttttcaa caatctcctt
      541 tgtgtctctt gctatcataa gttcttcatt tgttggaatg acaagaacct ttaccttgga
      601 atctttcgtt gatatttcat tatcgtcacc gcgcatattg tttttctgtt catccagggt
      661 tattccgaga tatccaagat actttgttac tatatttttt cttaacaagg gtgagttttc
      721 gccgacgcct gctgtaaaga ctataacgtc tacgccgttc atagcagcag tatatgcacc
      781 gatatactta gctatcctgt attcatagat atcgtgagca agagtgcata ttgcatcgcc
      841 attcatagct ccgttttcaa tatctctcat atcgctgaga ccgtttgtaa gaccggccat
      901 tcctgatttt ttatttagaa tatcgtttac ctgtttagaa gaaagtcctt cttcctgctg
      961 aagaaaagtt actatactcg ggtcaatatc tccgcatctg gttcccatta caagcccttc
     1021 cagaggagta aaacccattg aagtgtctac tgaccttcca ttgcatactg ccgcaacaga
     1081 tccgccgttt cctacatgta cggtaattat tttgagtgtc ctaacgtctt ttcccaaaaa
     1141 ttttgcagcc ctttgcgata catatctgtg acttgtgccg tgaaaaccat atctgcgaac
     1201 cttgtactta tcgtagtact cttttggaat ggcatacata aatgattttt caggcatggt
     1261 ctgatggaag gcggtgtcaa aaacacctat ctgcggaaca tttggcaaaa ttctcatggc
     1321 tgcttttata ccggttatat ttgcgggatt atggagagga gcgagaaagg aaagcatttc
     1381 cattgtattt aacatctcag agttgattct taccgaggac gcaaactttt ctccgccgtg
     1441 cacaactctg tgaccaattg catctatttc atccagagag ttcagtactc ccagatcctt
     1501 tgatacaaga atatcaagaa ccaattttag acccttttca tgatctttga tatcatcgtt
     1561 taaggtagtc tttttaccgt ttacgctatg aactattctt gaaccgtcaa tacctattcg
     1621 ttcaacaaga cctttagcaa tgacattttc ggtatccatg tccagaagct gatattttat
     1681 ggatgaactt ccgcagttaa taacgagtat cttcatcttt tatcctcctt ggaattaatt
     1741 tcttcatata cacaatatat attatagact aaataaataa taaatcaatt aaggctgtga
     1801 gttttcccgt gtaaataatc gacgaaaaag tgaacgacct ctcattttct tttaaaaaca
     1861 tataaaactc aactaccata cattaataaa tgatataatt aataaaagac aaaaagtata
     1921 agatccgggg gtgcaaaaag tgctggtttc tctttctctg aaaaattttg ggctgtttaa
     1981 ggatgaaaac atagagtttt cggacgggct taacataata agcggtgagt caggtgcagg
     2041 aaaatcaatg tttataagtg caataaactc gcttataaca ggacagtttc catctgagct
     2101 caagaaccag gaaggtatga tctctgcatt ctttagcgtg aacgatacag taaaaaaaat
     2161 tattttccaa gcaacgggga ttgagtcgaa tgaactggtt atttctgcaa acttctcatc
     2221 aaaaaagaca ctttacagaa ttaacggcat ggtagttact aaaaacataa tagaagaaat
     2281 aggcaagtat ctcattgaga ttcactctca ggaatcaagc gccatactgc gtgatcctct
     2341 ttatcagaat aagcttatgt acgatatcat aataaataag catagagagg cttttactga
     2401 gtataacagt ctttacagtg agtacagcat gctcaaggaa aaagctgccg ccataccttc
     2461 ggatccgtct gaagtactta ggaagataga tacactcaac tttcaaataa atgagataaa
     2521 aaaaacagat ccccagccca atgaagacac tgagctttcc gaaagattca gggctttgaa
     2581 caacgtacag gaaataaaga acaatctcta tgaaaatatg cagctgctga aagattccga
     2641 agagttcaac acagacaatt caataggtac ggttgcatac aatctttcta aaataaggga
     2701 gtacggattt aaagatacgg agcagtttgc cataagtatt cagcagcagc tccttgag
//
LOCUS       DAOSHR010000250         2750 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_35269_length_2750_cov_2.956215, whole genome shotgun sequence.
ACCESSION   DAOSHR010000250 DAOSHR010000000
VERSION     DAOSHR010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35269_length_2750_cov_2.956215"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="PLU28_10225"
     CDS             <1..444
                     /locus_tag="PLU28_10225"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOG35475.1"
                     /translation="FLGQFYKTIILYRFVRDINLLISSGLTLYEAMDKTVESIDSVLA
                     KKAFFSPGVDIRSGLDVKEAFCKVKDFKEISLAFALSGKVGGYRQVLDFLQKDCQSRI
                     KESSEKILKMIEPVLIILISVLIMSIAYEVYSKVFIGNINFSVGE"
     gene            452..850
                     /locus_tag="PLU28_10230"
     CDS             452..850
                     /locus_tag="PLU28_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35476.1"
                     /translation="MKPVDFRKGFLLLELSIYLIAEVIMFSSLCAVSILPLYRYFERL
                     SDQVLAVKTINRMRTVSFISRKADIYVSKTSILAGFRNNYFTFKINQYSYRELTFDDG
                     YIKSPGGIIVDRKNQTILKVVPVTAAVTLP"
     gene            847..1293
                     /locus_tag="PLU28_10235"
     CDS             847..1293
                     /locus_tag="PLU28_10235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35477.1"
                     /translation="MKKIIFLKYKGKKIGVGHMDCYIQSYQTEQHLLKDLFKMGISKS
                     VGYFEMGSWDEKYYLIYDKSHWLKKKLSPVQDVFIPQQLLAIDILLKMPSGEYVIKFP
                     SCSYVFERNKYYVKNGIICDRNEYDVSFSVQDINKAVMEYAVKGVL"
     gene            1290..1688
                     /locus_tag="PLU28_10240"
     CDS             1290..1688
                     /locus_tag="PLU28_10240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35478.1"
                     /translation="MIFSVKSLSRKPFLLLLDLLIILLAVMTAYSFFGGVSSKKKASD
                     EKKYEKTRYEQYLKSIREEKNEISEFFGFLEKYDARLIEFTYLSGRIESTVMLELSEG
                     QEIITKYSVKKISELKTGEKSIVFLSIFTE"
     gene            1698..2294
                     /locus_tag="PLU28_10245"
     CDS             1698..2294
                     /locus_tag="PLU28_10245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35479.1"
                     /translation="MRIRALVVGVLLSVFLILSILSKKEPYKEQIFILRSIGSGSINR
                     KFSNKELKKFMSAVLADDSKVESIKLLRDNSSVNNISTDFTLQVKQTLPEEDEFAYKT
                     LVCTESKEIAQSIFDGKTFSLEYLWSMKDSFKSFMPDCIRFAGVRVKKGVKSAYIYFN
                     DELLNVCEGEAIGNRQIMGIFDDGILVLTPEKEVEVIL"
     gene            2291..>2750
                     /locus_tag="PLU28_10250"
     CDS             2291..>2750
                     /locus_tag="PLU28_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35480.1"
                     /translation="MKKSFLVIGLFLLTVLSFTMSIDYDRKTNANIILFDFEKEYTLV
                     SNSELTVHKILFGEELFIPQSYKIEKGPLRSVEILSNMLEIRTLMPVKPLVDGKNRLI
                     FNGRGALNFEKLFLKEIRLGDLLNLFSQELKLNWIRMEPFPQEKLSLWSDE"
BASE COUNT          904 a          398 c          606 g          842 t
ORIGIN      
        1 tttttgggtc agttttacaa gacaataatc ttatatcgtt ttgtcaggga tataaatttg
       61 cttatttcca gcggacttac tctttatgag gctatggaca aaactgttga aagtatcgat
      121 tcggttctgg caaaaaaagc atttttcagt cccggagtgg atatacgcag cggtcttgat
      181 gtaaaagaag ctttctgtaa ggtaaaagat tttaaagaga tttcacttgc atttgccctc
      241 tcgggaaagg tgggcggtta cagacaggtt cttgattttc ttcaaaaaga ctgtcagagc
      301 agaatcaaag aaagcagtga aaaaattcta aaaatgattg aacctgttct cataatactt
      361 atttccgtac tcattatgag tattgcctac gaggtttaca gcaaggtgtt tataggaaac
      421 ataaattttt cagttggaga gtaggtgaag tatgaagcct gttgatttca gaaaaggttt
      481 tttgctactg gaactgagta tatatctcat tgcggaagtg ataatgttct catctctttg
      541 tgccgtaagc attcttcctt tgtataggta ctttgaaagg ctttctgacc aggtgctggc
      601 cgtaaaaacg ataaaccgta tgagaaccgt atcgtttatt tcccgcaaag cagatattta
      661 tgtaagtaaa acatcaattc ttgccggatt cagaaataat tactttacat tcaagattaa
      721 tcaatactcc tacagagagc tgacctttga tgacgggtac ataaaaagtc ccggaggaat
      781 tatagttgac agaaaaaatc aaaccatact gaaagttgtt cctgtaaccg cagcggtgac
      841 gctgccatga aaaaaataat atttttaaag tacaagggaa aaaagatagg agtaggtcat
      901 atggactgtt acatacagtc ttaccagaca gaacagcatc ttttgaagga tctttttaaa
      961 atgggtatca gtaaaagtgt tgggtacttt gaaatgggaa gctgggatga aaagtactat
     1021 ctgatctacg ataagtcaca ttggcttaaa aaaaaattat cgcctgttca ggatgttttt
     1081 attcctcaac agcttctggc aatagatatt ttgcttaaaa tgccgtcggg ggagtacgtt
     1141 ataaaatttc cttcttgttc atatgttttt gaaagaaata agtactatgt taaaaatggg
     1201 attatctgtg atagaaatga gtatgatgtt tctttttctg tgcaggatat taataaagca
     1261 gtgatggagt atgctgtaaa gggtgttcta tgattttttc tgtaaagagt ttatcaagga
     1321 aaccgttttt attgctgctg gatttattaa taatactact ggcggttatg accgcatact
     1381 ctttttttgg cggagtatcg agcaaaaaaa aggcctctga tgaaaaaaaa tacgaaaaga
     1441 ccagatatga gcagtatctc aaaagtatca gggaagaaaa gaatgagata tctgagtttt
     1501 tcggatttct tgaaaagtat gatgccagac tgatagagtt tacatatttg agcggaagga
     1561 tagagagcac ggttatgctt gagctgtcag aaggtcagga gataatcaca aagtatagcg
     1621 taaaaaagat ttctgagttg aaaacgggag aaaagagcat agtctttctc agtatattta
     1681 ctgaataagg agaggttatg agaataaggg ctttggtggt cggagtactg ctgtctgtct
     1741 ttttgatact ttcaattctt tccaagaaag aaccttataa ggagcagatc tttatactgc
     1801 gcagtatagg cagcggttcg ataaacagaa aattttcaaa taaagaactg aaaaaattta
     1861 tgagcgctgt tttggctgac gactcaaagg tcgagagtat aaaactgctc agggataaca
     1921 gcagtgtgaa taatatctct actgacttta ccctgcaggt taaacaaact ctgccagaag
     1981 aagatgagtt tgcttataaa acacttgttt gtactgaaag taaagaaata gcacagagta
     2041 tatttgacgg aaagaccttt tctctggagt atctgtggtc gatgaaggat tcattcaaga
     2101 gctttatgcc agattgtata agatttgccg gagtaagggt gaaaaaggga gtaaagagtg
     2161 cgtatatcta ctttaacgat gaacttttga atgtatgtga gggagaagct ataggtaaca
     2221 gacagattat gggaatattc gatgacggga tacttgtact tactccagaa aaagaggttg
     2281 aggtaatctt atgaaaaaaa gtttcctggt aataggctta tttcttttga cggttctttc
     2341 ttttacgatg agcatagact acgatagaaa aacaaatgcc aacataatcc tgtttgactt
     2401 tgaaaaggag tatacgttgg tttcaaacag tgaactgacg gttcataaga tacttttcgg
     2461 agaagaactg tttataccgc agtcatataa aattgaaaaa ggccctttga gatctgtcga
     2521 aatactttcc aatatgctgg agataagaac tctcatgccg gtaaaaccgt tagttgatgg
     2581 taaaaaccgt ctgatattca acggaagagg ggctttaaac tttgaaaaac tttttttaaa
     2641 agaaattagg ctgggagatc ttcttaatct tttttcacag gagttgaaac tcaattggat
     2701 aaggatggag ccatttcctc aggaaaaatt gagtttgtgg tcagatgaga
//
LOCUS       DAOSHR010000251         2690 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_36417_length_2690_cov_5.992789, whole genome shotgun sequence.
ACCESSION   DAOSHR010000251 DAOSHR010000000
VERSION     DAOSHR010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36417_length_2690_cov_5.992789"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..446
                     /locus_tag="PLU28_10255"
     CDS             <1..446
                     /locus_tag="PLU28_10255"
                     /inference="COORDINATES: protein motif:HMM:NF025066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HOG35481.1"
                     /translation="SNDLDKVLASFIIANGALSMGKKVTMFFTFWGLNVLRKSNFKAP
                     DKTFIEKMFGFMMPKGSEKLKLSKMNMMGIGTSMMKKVMQKKNVDSLELLMKKALENG
                     LTIIACQMSMDIMGIKQQELIDGIQVAGVATYLAEAEDSNVNLFI"
     gene            557..1897
                     /locus_tag="PLU28_10260"
     CDS             557..1897
                     /locus_tag="PLU28_10260"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HOG35482.1"
                     /translation="MNHNFPDDFIWGAATASFQIEGAAYGFGKLPSIWDTFSHTPGNV
                     ANGDTGDFACDHYNRFVQDIAIMKELGIKAYRFSISWPRVMKDAKNTPNPQGIEFYKN
                     LTDELIKNDIKPVATLYHWDLPQILQDEGGWANKKTAEYFVNFADLMFRELDGRVYQW
                     ITFNEPSVFIFLGNLDGVHAPGMKDIWTSAKCVHNVLWAHGSAVKIFREKGYKGQIGI
                     TLNLAPYEPASSEINDVMAASTGDAQWNRLFLDPIFKGTYPEIIMNNPEFRKVLGTLD
                     SHEMEVISEKIDFLGINYYFRNVVKYSQKGKLGFESVKMDGEKTYFGWEVYPQGLYDI
                     LRYIKENYGNIPLYITENGATYDDILTSDRKIHDVQRISYLKRHFEKALRAIQDGINL
                     KGYYVWSLLDNFEWAEGYSKRFGLVYIDYNTFERIPKDSFLWYKKVIEENTFEV"
     gene            1949..>2690
                     /locus_tag="PLU28_10265"
     CDS             1949..>2690
                     /locus_tag="PLU28_10265"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOG35483.1"
                     /translation="MKSIRGKILIGFVGASVLVFSVLAVILYIMVGQAVQPIIHDMSN
                     QILNARASELGRVMENAVKSVKSYSNNDMVKTLYWGMVSNTLENELKSNDIFDAFFLS
                     GLDGQAYGTGGLLFDVSTEGYFDSIINGSEVEITKPYISAQTGKAVFNIVVPIVNYSL
                     SGVFGGIISIDKLVEIASQINIRGGYGWIADNTGLVIVHPDQSVMLKMNLYKGKDFGF
                     TDLDKFVKDIFSGKSGFSFVNNEKTGKSV"
BASE COUNT          880 a          408 c          620 g          782 t
ORIGIN      
        1 tcagcaatga tcttgacaag gtgcttgcaa gttttataat tgcaaacggt gctctatcaa
       61 tggggaaaaa agttacgatg ttttttacat tctgggggtt gaatgttctg agaaaaagta
      121 attttaaagc tcctgataag acttttatag aaaagatgtt tgggtttatg atgcccaagg
      181 gttcagaaaa actcaagctt tctaaaatga atatgatggg tattggaaca agcatgatga
      241 aaaaagtcat gcaaaagaaa aacgttgaca gtcttgagtt gcttatgaaa aaagcgttag
      301 aaaatgggct taccataatt gcatgtcaga tgtccatgga cataatgggt ataaaacaac
      361 aggagctgat agatggaata caggttgcag gagtggctac ataccttgct gaagccgagg
      421 attcaaatgt aaacctcttt atataggttt gagtcggaac ggatgttccg actttatttt
      481 tttgcttgaa taataaaaag aattaacgta tacttagtat gtagaaacct aaatttataa
      541 tgaaggagga agtccgatga atcataactt tcctgatgat tttatatggg gagcagcaac
      601 ggcgagtttt cagattgagg gtgcagctta cggctttggt aaacttcctt ccatttggga
      661 cacattcagt cacactcctg gaaatgtagc taacggagat acaggagatt ttgcatgcga
      721 ccattacaac agatttgttc aggatatagc gataatgaag gagcttggga taaaagcata
      781 tagattttct atatcatggc ccagagtcat gaaggatgca aaaaatactc ctaatcccca
      841 aggaatagag ttttataaaa acctaaccga tgaacttata aaaaacgaca taaaaccggt
      901 tgcaactcta tatcattggg atcttccaca gattcttcag gatgagggag gatgggctaa
      961 caaaaaaaca gcggagtatt ttgtcaactt tgccgacctt atgttcagag agcttgatgg
     1021 aagagtatat cagtggatta cctttaacga accttcggta tttatatttc taggaaatct
     1081 agacggtgtt cacgcaccgg gcatgaaaga tatatggaca tccgccaagt gtgtccataa
     1141 tgtgctttgg gcgcacggat cggctgtaaa aatcttcagg gagaaagggt ataaagggca
     1201 gatagggata accttgaacc ttgctccgta tgaaccggca agtagtgaaa tcaatgatgt
     1261 tatggctgct tctacaggtg atgcccaatg gaacaggctg tttcttgatc ctatattcaa
     1321 aggaacttat cctgaaataa ttatgaacaa tccggagttt agaaaagtac tgggaacgtt
     1381 agattcccat gaaatggaag taatctcgga aaaaatagat tttcttggaa taaactatta
     1441 tttcagaaat gtggtaaaat attctcagaa aggtaaatta ggatttgaat ctgtaaaaat
     1501 ggacggagaa aaaacgtact ttggctggga ggtttatcct caggggttat atgatattct
     1561 taggtacata aaagaaaatt acggcaatat tccgctctac ataactgaaa atggtgcaac
     1621 atacgacgat attcttacat cagacagaaa gatacatgat gttcagagga taagttatct
     1681 caaacgtcat tttgaaaaag ccctccgagc cattcaggac gggataaatc tgaaaggtta
     1741 ctatgtttgg tctctgttgg acaactttga gtgggcagaa ggttattcca agagattcgg
     1801 acttgtatat atagactaca atacatttga aagaatacct aaagatagtt ttttatggta
     1861 taagaaagtt attgaagaaa atacctttga ggtttaaatt aataaaactg cccctcctgt
     1921 gggtggtttt attttatagg gggataaaat gaaaagtata agaggaaaga tacttattgg
     1981 atttgtgggg gcaagcgttc ttgttttttc agtgcttgca gtgattcttt atattatggt
     2041 tggacaggct gttcagccta taatacacga tatgtccaat cagatattaa atgcaagggc
     2101 gtcggagctg ggaagggtta tggaaaatgc tgttaagagt gtaaaaagct attccaacaa
     2161 tgacatggta aagactcttt actgggggat ggtgagtaat actcttgaga atgagcttaa
     2221 gtccaacgat atttttgatg cgttttttct aagtggtctt gacgggcagg cttacggaac
     2281 cggaggactt ttattcgatg tttctacaga agggtacttt gacagtatca taaacgggag
     2341 cgaagtcgaa attaccaaac cgtatatttc ggctcagacg ggcaaggcag tcttcaatat
     2401 tgtggttccg atagttaact atagccttag cggtgtattc ggaggcatca tcagtattga
     2461 caagcttgtc gagatagcct cacagataaa cataagagga ggctatggat ggattgccga
     2521 taatacaggt cttgtgatag ttcaccctga tcagtcagtg atgctgaaga tgaaccttta
     2581 taagggtaaa gattttggat tcacagatct ggacaaattt gtaaaagaca tattttccgg
     2641 taagtcaggt ttttcctttg tgaataatga aaaaacagga aaatctgtaa
//
LOCUS       DAOSHR010000252         2671 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_36807_length_2671_cov_4.859709, whole genome shotgun sequence.
ACCESSION   DAOSHR010000252 DAOSHR010000000
VERSION     DAOSHR010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36807_length_2671_cov_4.859709"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1183
                     /locus_tag="PLU28_10270"
     CDS             <1..1183
                     /locus_tag="PLU28_10270"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOG35484.1"
                     /translation="DFVAKGIIWAVDHGAEVLSNSWGGKGYSYLLHSAINYALANNSV
                     FVASAGNDTTDEIHYPSCYPGVINIAASNAADGITSFSTRGEWVTVSAPGEKILSTIP
                     VWDGAYGYMDGTSMATPHVSGAVVALISKIKKASKTYTPYQIRQMLINSAVDICKPGF
                     DNDSGWGRIDLEAAAKIDVDTMPKGATVTYIVKGERLVSVSETSQYPNGFPLTGAYIT
                     LKPLTPNSPVLYGRTAFDKTASTTYVTFSEVVPGDYTMILSSGNMNGFMAMTGVLAQS
                     YKKSNETLVANTNTSVTKTLKSSPTVTVNSSSPSVSSNFKLTYTDGPIDVSGTTLTFN
                     DTYKAGFYDIFITTPSPAATDTTFDVSISYPGLTLSDTFNASLTMGSTLSVNPLPVYI
                     Y"
     gene            complement(1206..1745)
                     /gene="rfbC"
                     /locus_tag="PLU28_10275"
     CDS             complement(1206..1745)
                     /gene="rfbC"
                     /locus_tag="PLU28_10275"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HOG35485.1"
                     /translation="MFIKKEELPMGLMLLHTERFKDSRGFFIESFNRDDIKKIGIKEE
                     FILDCISSSHFGVIRGLHFQRYKPQSKLLRVLTGCILDVAVDIRKESPSFLKYFSVEL
                     NGDDDTLLYIPKGFAHGFLALSEPAVVLYKADGYYDPKDESGIIWNDRQLGINWKLRE
                     NNIQHPVLSERDKNLKPLP"
     gene            complement(1761..2615)
                     /locus_tag="PLU28_10280"
     CDS             complement(1761..2615)
                     /locus_tag="PLU28_10280"
                     /EC_number="1.8.4.11"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF004042.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methionine sulfoxide reductase B/A
                     protein"
                     /protein_id="HOG35486.1"
                     /translation="MKFIPRHLDENERSVILNKNTEKPFSGRYNDFYESGTYICKQCG
                     LPLFRSEDKFSSGCGWPSFDDEIPSAIERKPEKDGRTEITCSFCGAHLGHVFEGENFT
                     TKNIRHCVNSISLDFIQAGNEMVLQRALFAGGCFWGVEHLFKDIEGVLQTKVGYSGGT
                     IENPSYDEVCSGGSGHTESVLIIYDSTKISYEKLIKIFFEIHDFTQLNRQGPDFGEQY
                     RSVVFCFDRGQMETALKITGELKKAGYDVKTELKTAVNFFPAEEYHQRYYEKTGKKPY
                     CHYRRKIF"
BASE COUNT          775 a          598 c          465 g          833 t
ORIGIN      
        1 cgattttgtg gccaaaggaa taatatgggc agttgaccac ggagctgagg tcctttcaaa
       61 ctcatggggc ggaaaaggat attcttatct gcttcattcc gctataaact atgctcttgc
      121 aaataacagc gtatttgtag cttccgccgg aaacgatact accgatgaaa ttcattatcc
      181 gtcatgttat cccggagtta taaatattgc tgcaagcaat gctgctgacg gaataacctc
      241 tttctctacc agaggagaat gggtaacggt atctgcaccg ggtgaaaaaa ttctctcaac
      301 aattcctgta tgggacggag catatggcta tatggatgga acatctatgg ctactcctca
      361 tgtttccggg gcagttgtag ctcttataag taaaatcaaa aaagcttcca aaacttatac
      421 tccataccag ataagacaga tgcttatcaa ctctgccgta gacatatgta aaccaggttt
      481 tgacaatgac agcggttggg gaagaataga ccttgaggca gctgcaaaaa tagatgttga
      541 tacaatgcct aaaggtgcaa cagtaacgta catagtaaaa ggtgaaagat tagtttctgt
      601 atctgaaact tcacagtatc caaacggttt ccctcttaca ggcgcttata taactcttaa
      661 acctttgaca ccaaattcac ctgttcttta tggaagaacc gctttcgata agacggcttc
      721 cacaacatat gtaactttta gcgaagttgt tcccggagat tacactatga ttctttcttc
      781 aggaaatatg aacggattta tggcaatgac aggagtcctt gcacagagct ataaaaaatc
      841 taacgaaaca ctcgttgcaa atacaaatac atctgtgacc aagaccctaa aatcatcacc
      901 gactgttact gtaaattcgt cttctccatc agtatcaagc aactttaagc tgacttacac
      961 agacgggcct attgatgtaa gcggtactac tcttactttt aacgatactt ataaggccgg
     1021 attctatgat atattcataa ctaccccatc accggcagcc acagatacaa cgtttgatgt
     1081 aagcatctct tatccgggac ttacactctc agatacattt aatgcatctc ttacaatggg
     1141 tagcactctc tcggttaatc ctttaccagt atatatctat taaatactaa taactgaccc
     1201 cttccttacg gaaggggttt taagttttta tctctttctg aaagaaccgg atgctgtatg
     1261 ttattttctc tgagtttcca gttaatgcca agttgcctgt cgttccatat tataccgctt
     1321 tcatctttgg gatcgtaata accatcagct ttataaagca caacggccgg ttcggacaga
     1381 gcaagaaaac cgtgtgcaaa ccccttaggt atataaagta aagtatcgtc atccccatta
     1441 agctctaccg aaaagtactt caagaagctc ggtgactcct ttcttatatc caccgccaca
     1501 tccagtatgc atcctgtcag aactctaagc agcttgctct gaggcttgta tctctgaaaa
     1561 tgaagacctc ttataacccc aaaatgcgag gaagatatac agtccagtat aaactcttct
     1621 tttattccga ttttttttat atcatcacgg ttgaagcttt caataaaaaa tcccctgctg
     1681 tctttaaacc tttctgtgtg aagaagcatc agtcccatgg gaagttcttc cttttttata
     1741 aacactgtgt tctccatcct ttaaaaaatt tttcttctgt aatggcagta tggtttttta
     1801 cctgtcttct cataatacct ctgatggtac tcttcagcag gaaaaaaatt cacagccgtt
     1861 ttaagctctg ttttgacatc atatccagcc ttttttagct ctcctgttat ctttaaagct
     1921 gtttccatct gtccgcggtc aaagcaaaac acaacacttc tgtactgttc cccaaaatct
     1981 ggaccctgtc tgttcagttg tgtaaaatcg tgtatttcaa aaaagatttt tattagcttt
     2041 tcataactta tctttgtact gtcataaata ataagtacag actccgtatg tccgctacca
     2101 cctgaacaga cttcatcata ggatggattt tcaattgttc ctccgctgta tccgactttg
     2161 gtttgtagaa caccctctat atctttgaat aagtgttcaa ctccccaaaa gcagcctccg
     2221 gcaaacaacg ccctctgaag aaccatttca ttaccggcct gtataaaatc aagcgaaatt
     2281 gagtttacac aatgacggat gtttttggtt gtaaagttct ctccttcaaa aacatgtccg
     2341 agatgagcac cacagaaaga acatgttatt tcagttcttc cgtctttttc aggttttctc
     2401 tctatggcag agggtatctc gtcatcaaaa ctcggccatc cgcaccccga actgaactta
     2461 tcttcagatc tgaagagcgg aaggccgcat tgtttacata tataggttcc actttcataa
     2521 aaatcgttgt atctgccgct gaacggtttt tcagtatttt tgttaagtat cacagatctt
     2581 tcgttttcat caagatgcct aggtataaac ttcataacac cacctcctta attgggagtt
     2641 tttttgtcgg tgttaaaatt ataccataac a
//
LOCUS       DAOSHR010000253         2669 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_36845_length_2669_cov_4.987376, whole genome shotgun sequence.
ACCESSION   DAOSHR010000253 DAOSHR010000000
VERSION     DAOSHR010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36845_length_2669_cov_4.987376"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1515
                     /gene="priA"
                     /locus_tag="PLU28_10285"
     CDS             <1..1515
                     /gene="priA"
                     /locus_tag="PLU28_10285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOG35487.1"
                     /translation="THLLYGVTGSGKTEVFLQLIEPLINNHEQILLMIPEISLIWQYV
                     QRLQHKFRTCRIGIYNSELTFKEKKSIMEKTMNGQTDIIIGTRSAVWLRFKKLGMIII
                     DEEHDSSFYQMEGNIYDSIHIAQIKKQIESSYLLLSSATPRIIDFFESSNGNFMIHRI
                     KKRYNVTLPDIELIDLSKEEKYNWIFSVKIIKSIKDVLDRNKKVIVFTPTKGYANYII
                     CTNCGHIFRCKNCDISMTYYKQENHLKCHYCDNTQPVPSVCPKCGSTELQTRGFGTER
                     VMNELIRIFPSQPMVRVDRQAVKSSQDVQETFKYINNPGRMIIIGTKMITKGLNIPDL
                     ELVVVLDCERYFNMPDYNASENSSALLMQVAGRSGRKERGRVMIQSFNPKNELYKFIL
                     NHDYDSIISAETENRKKYLYPPFTNLIIILVKDSQEENLNKKALKIYKSLCSDLDRKM
                     LLGPVKPSVYKLNNFFRLSIIVKISTLDLQIFRQIYSEYRNSVSIYINPPTTMI"
     gene            1586..2134
                     /gene="raiA"
                     /locus_tag="PLU28_10290"
     CDS             1586..2134
                     /gene="raiA"
                     /locus_tag="PLU28_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOG35488.1"
                     /translation="MDYKVLARDFQLTPAIEEYLEKRMAKPDRLLRVHGDLISVPDVK
                     IEKEGGIYKVEITAHFKKINKIIKVEQRGGDLYEIIDNVTDSFERKINKMKTKLQEHF
                     SDTVEYDIPPRKDAFVQDKSMDIIKRQKRYDLSIMTVDEAVLQIDMLGHQFFVFKNAE
                     TDEVNAVYRRNDNSLGLIQFND"
     gene            complement(2160..2396)
                     /locus_tag="PLU28_10295"
     CDS             complement(2160..2396)
                     /locus_tag="PLU28_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HOG35489.1"
                     /translation="MAKVTVYSTPSCPWCTRVKRYLSEHGISYSDYDVSKDKAAAEKM
                     VKRTGQMGVPVLEIGSNIVIGFDKNKIDRLLSIK"
     gene            2484..>2669
                     /locus_tag="PLU28_10300"
     CDS             2484..>2669
                     /locus_tag="PLU28_10300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35490.1"
                     /translation="MYIYEYEKKQTEYQINMQDGYKTVRFGAGYKDEKCKANETVHLH
                     IFEPKEMKGDILFLHGIG"
BASE COUNT          962 a          499 c          491 g          717 t
ORIGIN      
        1 acccatttac tttacggagt taccggaagc ggaaaaacag aagtctttct tcagcttata
       61 gaacctctca ttaataatca tgagcagatt cttctgatga ttccggaaat atcgcttata
      121 tggcagtatg ttcagagact gcagcacaag ttcagaacct gccgtatagg tatctataac
      181 tctgagctta ctttcaagga aaagaaaagt ataatggaaa aaaccatgaa cggtcagaca
      241 gatataataa taggaacaag gagtgcggta tggctgaggt ttaaaaagct tggaatgata
      301 ataattgatg aagagcacga cagttcattt tatcagatgg aaggaaatat ctacgacagt
      361 atacacattg ctcagattaa aaaacagatt gaaagctcct atcttttact atcaagcgct
      421 actcccagga taatagattt ttttgaaagc agtaacggaa attttatgat tcaccgcata
      481 aaaaaaagat acaatgtaac tcttcccgat attgaactga tagatctgtc aaaggaagaa
      541 aagtataact ggattttttc agttaaaatt attaagagca taaaagatgt tctggacaga
      601 aataaaaaag ttatcgtatt cacaccgact aaaggatacg ctaactatat aatatgtacc
      661 aactgtggac atattttcag atgcaaaaac tgtgacatct cgatgaccta ctataaacag
      721 gaaaaccact taaaatgtca ttactgcgat aatacccagc cggttccgtc agtatgccct
      781 aaatgtggct caaccgagct tcagacaaga ggcttcggta cagaaagagt tatgaacgag
      841 ctaataagaa tattcccatc ccagccaatg gtaagagtgg acagacaggc tgtaaaaagt
      901 tcgcaggatg tacaggaaac cttcaaatat ataaataatc ctggacgcat gataataatc
      961 gggacaaaaa tgataaccaa aggactcaac attcccgatc tggaactggt tgtagttctg
     1021 gattgcgaaa gatacttcaa tatgcccgat tataacgctt ctgaaaacag ttcggctctt
     1081 ttaatgcagg ttgctggaag atcaggccgg aaagaacgag gacgggtaat gatacaaagc
     1141 ttcaatccaa aaaacgaact ttataaattt atattaaacc atgactatga ctcaattatc
     1201 tcagccgaaa cagaaaacag gaaaaagtac ctgtaccctc cttttacaaa tcttataatt
     1261 atccttgtaa aagactcaca ggaagaaaac ctaaataaaa aagctcttaa gatatataaa
     1321 agcctttgct cagacttaga cagaaagatg ctcctcggac cggtcaaacc ttctgtatac
     1381 aaactgaata actttttcag actaagtata atcgtcaaaa tcagtaccct ggatctgcag
     1441 atattcaggc agatctactc cgaatacaga aactctgtta gtatttacat aaatcctccg
     1501 actaccatga tttaacaaag tttgacttat aagccgctta gtggtatcat ataagtaagc
     1561 accaatactc tacggaggtg attttatgga ttataaagtt ttggcaagag atttccagtt
     1621 gactccggca attgaagagt accttgaaaa aagaatggct aagccagaca gactacttag
     1681 agttcatggt gacttgattt ccgttcccga tgttaagatc gaaaaagaag gaggtatata
     1741 taaggtagag ataacggccc acttcaaaaa aataaacaaa atcataaaag ttgaacagcg
     1801 cggcggcgat ctttacgaga ttatagacaa cgttaccgat tcttttgaaa gaaagattaa
     1861 caaaatgaag accaaactcc aggaacattt ttccgatact gtcgagtacg atataccgcc
     1921 gagaaaagat gcttttgtac aggataaaag tatggacatt ataaaaagac aaaaaaggta
     1981 cgatctgtcc attatgacag tagacgaagc ggttcttcag atagatatgc ttggccacca
     2041 gtttttcgtt ttcaaaaacg cagagaccga tgaagtaaac gcagtctaca gaagaaatga
     2101 caattcgctt ggtttgatac agttcaatga ctaataaaag gcggcttaag ccgccttttt
     2161 tattttatcg aaagtaacct gtcaatcttg tttttatcaa aacctattac tatattgctg
     2221 cctatctcaa gaacaggcac tcccatctgg ccggtgcgtt tcaccatttt ctctgccgca
     2281 gccttatcct ttgaaacatc ataatcactg tagcttatac catgttctga aagatatctt
     2341 tttacccttg tgcaccatgg gcacgaagga gttgaatata ctgtcacttt tgccataact
     2401 atcacctcac atacccctat agggtataat tttataatat tatatcatac attatctata
     2461 aaatcaatat acggagggtt catatgtaca tctatgaata tgagaaaaaa caaacagagt
     2521 atcagataaa catgcaggac ggttacaaaa cagtccgatt cggtgcggga tataaagatg
     2581 aaaaatgtaa agccaatgaa actgtacatc tacatatttt tgaaccaaaa gaaatgaagg
     2641 gcgatatcct tttcctccac ggaatagga
//
LOCUS       DAOSHR010000254         2666 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_36909_length_2666_cov_5.595557, whole genome shotgun sequence.
ACCESSION   DAOSHR010000254 DAOSHR010000000
VERSION     DAOSHR010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36909_length_2666_cov_5.595557"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1209
                     /locus_tag="PLU28_10305"
     CDS             <1..1209
                     /locus_tag="PLU28_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35491.1"
                     /translation="PITTGQVAYRINYVGVVQLIVSGQGNKEKTFVNSSKADLVKEAE
                     ELKGIRLELEISNPSDWIASVWGRIDIKSDSMKKIVASLNIDANDSEFVSPKKTRTLI
                     FNIDRVIPPGTYQVQFAMDYGYQDFYKGKTNFQTELLVPAEIEQSRKTLLLSTDPKEL
                     YVKIEKRASSRGVQVNPVNTNVLIVNSDYIDATVTPSLVNDFSAFAGVEGYFRLMDSK
                     YVTLRTTRDILSRAFASSSSPVTLTFDYRRAALDSLNGEYYGYLNLDASGKLSEKTLV
                     KNIQVPIIVDFGENNYKMTVSEKNVTLSEDKTTAHISLKLKNEGNSRIKYDMLVYKFN
                     SQTNQMVGESTKPYQNIYIYPDTEVTLNYDLALEDTVDKITVQIIHTMGTDTAGNPIT
                     KQESLDFKVK"
     gene            1233..2390
                     /locus_tag="PLU28_10310"
     CDS             1233..2390
                     /locus_tag="PLU28_10310"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35492.1"
                     /translation="MKKLTGLFLVIFFCGLVQAQDLANYSFFQTDIREAISNLSMDFE
                     TTILLDPNIAGYVTVEIKDKPFTKALEMILMPFGYAYKQIEDYYLVGVPDPSSGTSIH
                     LNDTFVMKTRYLNAKNLVQLLPRYLQKFVSFSEQDQSLVVINAPPKIAGVIANAIRKI
                     DMGRPEMIIEVKVVQLDTEVFRKWGIDELNIYNKASPAKYTRNSEKGLYFYDDLLEMI
                     FDLGNENFSLVLNKAITEGKAQVVANAKLSVISGMSGTISGKLIDPLKLTKEENDYTG
                     FSIKIVPFLMTGKKVRINADIELKDRRIVEKESNSIISNKLTTDFILNLDQEGTIGSF
                     NYSTSSQVEIGVPLISKIPGIGMFFKSKIEKEVEQQLIFLVNCHASGGEIQ"
     gene            2387..>2666
                     /locus_tag="PLU28_10315"
     CDS             2387..>2666
                     /locus_tag="PLU28_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="STN domain-containing protein"
                     /protein_id="HOG35493.1"
                     /translation="MKKLILMSMVVLSFCITYAFDISFYETEISKAIDQIATSQGVTI
                     IVGPEVKGKVTMTLMSISVESALELILYGTPYDYEKIAEGIYVVGSLDT"
BASE COUNT          908 a          506 c          503 g          749 t
ORIGIN      
        1 cctataacta ccggtcaggt agcctacagg ataaattatg ttggagtagt ccagcttatt
       61 gtgtcaggac agggcaacaa agaaaagacc tttgtaaact cttcgaaagc agatcttgta
      121 aaagaggcag aagaacttaa gggtatacgt ctggaacttg agatctctaa tcctagtgat
      181 tggatagcct cagtttgggg aagaattgat ataaaatctg attctatgaa aaaaatcgtt
      241 gcttccctca atatagatgc taatgatagt gagttcgtat ctcctaaaaa aacaagaact
      301 ctaattttca acattgacag agtaatacct cccggaacat accaggttca gtttgctatg
      361 gactatggat accaagactt ttacaaaggc aaaaccaact tccagaccga actcttagta
      421 cctgctgaaa tagaacagag cagaaagaca ttgcttcttt caacagatcc aaaagaacta
      481 tatgtgaaaa ttgagaaaag agcttcttca aggggagtac aggtaaatcc cgtcaatacc
      541 aatgttctta ttgtaaacag cgattacata gatgcaacgg taacaccttc tctagttaat
      601 gatttttctg cttttgccgg tgttgaaggc tattttaggc ttatggattc aaaatatgtt
      661 actcttagaa ctaccagaga tattctgtca cgggcttttg ccagctcatc gagtcccgtt
      721 actcttactt ttgattacag aagagcagca cttgattcac ttaatggcga atactacggc
      781 tatctcaacc ttgatgccag cggtaaactt tcagaaaaga ctctggtaaa gaatattcag
      841 gtacctatta tcgtagattt tggcgagaac aattataaaa tgaccgtaag cgaaaagaat
      901 gttactcttt cagaagataa aactactgcc catatatctt taaaacttaa aaatgaaggc
      961 aactcaagaa taaagtacga tatgcttgta tataagttca acagtcagac aaaccagatg
     1021 gtaggagaaa gtaccaaacc atatcagaat atctatatat atcctgatac agaagttact
     1081 ctgaattatg accttgcttt agaagatact gttgataaaa ttacagtcca gataatccat
     1141 accatgggaa cggatactgc aggcaatcct ataaccaaac aggaaagtct tgacttcaag
     1201 gtaaaataaa ctaccttttt gggagtgatt taatgaaaaa attaactgga ctatttctgg
     1261 ttatattttt ttgcggtcta gtacaggcac aggatctggc taactactct tttttccaga
     1321 ccgatatccg agaagccatt tcaaaccttt ctatggattt tgaaactaca attctattgg
     1381 acccgaatat agccggttat gtaacggtag aaataaaaga caagcctttt accaaagcac
     1441 ttgaaatgat tcttatgcct tttggatatg cttataaaca gattgaggat tactatcttg
     1501 taggagttcc ggatccatct tccggtacat ccatacacct caacgatacc tttgtcatga
     1561 aaacacgtta tcttaacgcc aaaaacctgg ttcaacttct tccaaggtat cttcagaagt
     1621 ttgtttcatt ttcagaacag gatcagagcc tagtggtgat aaatgctcct cccaaaattg
     1681 caggcgttat tgccaatgcc atacgtaaga ttgacatggg acgtcctgaa atgattatag
     1741 aggtaaaagt tgtacagctg gacacagaag tcttcagaaa atggggaata gatgaactca
     1801 atatctacaa taaggcttct cctgcaaaat atacaagaaa tagcgaaaaa ggtctttatt
     1861 tttatgatga tctactggaa atgatctttg atcttggcaa tgaaaacttt tcccttgttc
     1921 tgaacaaagc cataacagaa ggtaaagccc aggttgtggc taacgccaaa ttgagtgtca
     1981 tctccggaat gtccggaacc atatccggaa agctcataga tcctttaaag cttacaaaag
     2041 aagaaaatga ctataccggt ttcagcataa aaattgttcc tttcctgatg acaggaaaaa
     2101 aagtcaggat aaatgctgat attgagctta aagaccgtcg aatagttgaa aaagaaagca
     2161 attcaataat atccaacaaa ctcacgacag attttatact taatcttgac caggaaggaa
     2221 ccataggttc tttcaactac tccacatctt cacaagttga aataggagtt ccccttattt
     2281 caaagattcc cggaatagga atgtttttca agtccaagat agagaaagaa gtagaacagc
     2341 agctaatctt tctggttaac tgccatgcaa gcggtggtga aatccaatga aaaagctgat
     2401 cctcatgtcc atggtagtac tgagtttttg cataacatat gcatttgata taagttttta
     2461 cgaaactgag atatccaaag caatagacca gatagccaca tcccagggag tcactataat
     2521 agtcggaccg gaagtcaaag gcaaggtaac tatgacttta atgagcataa gcgtcgaatc
     2581 tgccctggag cttatccttt acggtactcc atacgactat gaaaagattg ccgaaggaat
     2641 atatgtagta ggctctcttg atacac
//
LOCUS       DAOSHR010000255         2666 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_36911_length_2666_cov_5.077365, whole genome shotgun sequence.
ACCESSION   DAOSHR010000255 DAOSHR010000000
VERSION     DAOSHR010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36911_length_2666_cov_5.077365"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..539
                     /gene="pth"
                     /locus_tag="PLU28_10320"
     CDS             <1..539
                     /gene="pth"
                     /locus_tag="PLU28_10320"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOG35494.1"
                     /translation="YVFNRHNVGFLCVDRYYELTKKTIDYKSFSFRTYEGFESKEFIL
                     VKPLTFMNLSGNIFSDLFQKHGKIMVQDIIVLYDDVSIPFGKIRIKEQGSAGGHNGMK
                     SIIGVLASDNFTRIRIGIGEKPEFMDLADYVLANFSQDEYKSLYQILDGACSAIDFLL
                     QGETSKAMNVFNNKDFLV"
     gene            573..1640
                     /locus_tag="PLU28_10325"
     CDS             573..1640
                     /locus_tag="PLU28_10325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35495.1"
                     /translation="MLKRIFLLILSAIIIVVLIFFIVQSGKVVRLAIMISPDTNLGYE
                     ELKAFEYFLSQPEYKNQSMVRVSFFKPKLEKQDMINALEKVKSFRPDILIAGAVSSEI
                     QMIYSELSSLKFPVILSTVSSERFSNKNDNVFRLVPSSNFLSEYSAKYLYEKGFKSVL
                     IARSAINPDYSDSVYEVFAQNFQGSIKYVDYDKFLDEPYDLYDCFFLVTGPEETMLLL
                     NGLKKEKGKGYYINSWAFDFLRNNYNGSFMQGTSVISDYVVDYQNNHNSIIEKFTEDY
                     GVKPTFASSVILTLLDFVFDAVNSKNITEFLKQEKKFENTVYGSFYYNNFGDSIPENI
                     FVYSYNNGTVRLTDTIEIIKQ"
     gene            1659..2150
                     /locus_tag="PLU28_10330"
     CDS             1659..2150
                     /locus_tag="PLU28_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35496.1"
                     /translation="MTFKKIFRRTIRNLFVQNIFMVMMGVMIWLIVMGLVFVNLSVIS
                     DVNTDSLQTTLKNYSSSLIAIYETSDPQRLNKFLDINSLFIIDKNGNIVRSVPEGFEG
                     INIKNSDFFRLVTSIEYQPSIFKYSSLSDGKLRVHFFLNIMICILLQTWTIITFIHHR
                     HTT"
     gene            2090..>2666
                     /locus_tag="PLU28_10335"
     CDS             2090..>2666
                     /locus_tag="PLU28_10335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35497.1"
                     /translation="MYLITDMDDNYFHSSSSYYMSLLKKDGTVLYSPNFFNITKVDPE
                     KFPRFFIRNARFIINKPVSWNSLYIVTSLDITREFIVMMILSAIITCLICWNLVSRKR
                     NMDNLVEFTQEFFSIKDSFDILSLKIKAIDKKLSPSNIDIDFGEVYDTITEKNFQFHE
                     ISSLKETLDITIKEFLKLFENLAASNEEIKAS"
BASE COUNT          881 a          460 c          470 g          855 t
ORIGIN      
        1 gttatgtctt taacagacat aacgtgggct ttttgtgtgt ggacagatac tatgaactca
       61 ccaagaaaac tatagattat aagtcttttt ctttcagaac ctatgaaggg tttgagagca
      121 aagagtttat tcttgtcaaa cctcttacct tcatgaacct ttccggaaac attttttccg
      181 atctctttca gaaacacgga aaaattatgg ttcaggatat tatcgttctc tatgatgatg
      241 tttccatacc tttcgggaaa atccgcataa aagaacaggg ctcggcaggc ggacacaacg
      301 gcatgaaatc cataatagga gttcttgcaa gcgataattt tacaagaata cgaataggaa
      361 taggtgaaaa acctgaattc atggatctag cggattatgt gcttgccaat ttttcacaag
      421 atgagtataa gtccctttat cagatacttg acggtgcatg ctctgccata gacttcctgc
      481 tgcaagggga aacctcaaaa gccatgaatg ttttcaataa taaagatttt ttggtataat
      541 tgatatgtga tttttttctg aaggagctga ttatgcttaa aagaatcttt ctcctcattc
      601 tgtccgcaat aataattgtt gttcttattt ttttcatagt gcaaagcgga aaagttgtca
      661 ggctggctat aatgatatcg cctgatacaa atcttgggta tgaggaatta aaggcatttg
      721 aatacttttt atctcaaccg gagtataaaa atcaatccat ggtacgggtt tcttttttta
      781 aacccaagct tgaaaaacag gatatgataa atgcattgga aaaagtcaag agtttcagac
      841 cggacattct tattgcaggc gctgtaagtt cagagattca gatgatatat tcggagcttt
      901 catcattgaa atttccagta atcttatcaa cggtcagttc cgaacgcttc agcaataaaa
      961 acgataatgt tttcagactg gtaccttctt caaatttttt atcggagtat tcggcaaaat
     1021 acctgtatga aaaaggattt aaatctgtat taatagcacg ttctgccata aatccagatt
     1081 attctgattc agtatacgaa gtttttgccc aaaattttca aggatcaata aagtacgttg
     1141 actacgacaa atttttggat gaaccttacg atctgtatga ctgttttttt cttgtaacag
     1201 gtccagagga aaccatgctg cttcttaacg gacttaaaaa agaaaaggga aaaggatatt
     1261 atataaacag ctgggctttt gactttttga gaaacaatta caatggttct ttcatgcagg
     1321 gtaccagcgt tataagcgac tatgttgtag attatcagaa caatcataac agcatcattg
     1381 aaaaatttac agaggattat ggtgtaaaac caacttttgc ttcgtctgta atacttacac
     1441 tgcttgattt tgtctttgat gcagtgaact ccaagaatat tacagagttt ttgaaacagg
     1501 aaaaaaagtt tgaaaatacc gtctatggtt ctttctatta taataatttt ggagacagta
     1561 ttccagaaaa catctttgta tacagctata ataacggcac tgtaagactc acggacacca
     1621 ttgagattat caaacagtag atacggagtt ggaaactaat gacattcaaa aaaattttca
     1681 gaagaaccat acgaaatctt tttgtccaaa atatcttcat ggttatgatg ggagtaatga
     1741 tctggctcat agtgatgggt ctggtttttg tgaacctctc agttatttcc gatgtcaaca
     1801 cagacagtct tcagactact ctgaagaact attcttcttc actgattgca atctacgaga
     1861 catctgatcc ccaaaggctg aataagtttc ttgatataaa ctccctattc atcatagata
     1921 agaacggaaa tatcgtaaga tcagtaccag aaggatttga aggaattaat attaagaaca
     1981 gtgacttttt caggcttgtt acctctattg aatatcagcc aagcatattt aaatactctt
     2041 ctctctcgga tggaaagctc cgggtacatt tttttctgaa tataatgata tgtatcttat
     2101 tacagacatg gacgataatt actttcattc atcatcgtca tactacatga gtcttctgaa
     2161 aaaagacgga acagttcttt actctccaaa cttctttaac attacaaaag tcgatccaga
     2221 aaaatttccc cgttttttca taagaaatgc caggtttata ataaacaaac ctgtaagctg
     2281 gaacagtctt tatattgtga cttcgctgga tataacacgc gaatttatag ttatgatgat
     2341 tctttcagct ataataacct gtcttatatg ctggaatctg gtttcgcgaa aacgcaacat
     2401 ggacaacctc gtagaattca cacaggagtt tttcagtata aaggacagtt ttgatatttt
     2461 gtccctcaaa atcaaggcaa tcgataaaaa actttctcca tcaaatatag atattgattt
     2521 cggagaagtt tatgatacca taacggaaaa aaacttccaa tttcatgaaa tatcaagtct
     2581 taaggaaact cttgatatta caataaaaga atttttaaaa ctatttgaga atcttgctgc
     2641 ttcaaacgaa gagataaaag cctcca
//
LOCUS       DAOSHR010000256         2643 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_37385_length_2643_cov_6.442040, whole genome shotgun sequence.
ACCESSION   DAOSHR010000256 DAOSHR010000000
VERSION     DAOSHR010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37385_length_2643_cov_6.442040"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="PLU28_10340"
     CDS             <1..299
                     /locus_tag="PLU28_10340"
                     /inference="COORDINATES: protein motif:HMM:NF014838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HOG35498.1"
                     /translation="NRLPIIAKLKVCPVTIRKTDKTEEKLIVHGGIVDMSGKELIILT
                     TKAERFEEIDTLKTKEMIDKLEKEVTTATQDREKIKNELETNRIRYKYAQTVKK"
     gene            complement(347..1375)
                     /locus_tag="PLU28_10345"
     CDS             complement(347..1375)
                     /locus_tag="PLU28_10345"
                     /inference="COORDINATES: protein motif:HMM:NF012926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HOG35499.1"
                     /translation="MNNFLNSLDIIKKYQDSSGAYVASPNFSQYGFCWFRDSSFIARA
                     MYRAGESICARKFFDWAVKVIRNNRPDIEKVLLKDKSELTHKDLLPTRYRVDGSANND
                     DWPNGQSDGYGTFLWAAHECGYEVPADVSKMVVNYIEKIWEVPCYDAWEEGPEYLHTS
                     TLFSLAAGLKAQSIATGQKNCYELILQKVLTQHTKNGRFVKSTGNDCVDSSLLWCSYP
                     YALYSNGNELVINTVSEIERTLKKGCGLKRYLNDTYFGAGSWTLLSANLGSYYARNGE
                     KEKALEIKRWLDTNFDSNGFLAEQIAENLLAPDMYEPWVKRWGNIASPLLWAHAEYIN
                     LCLDIQNS"
     gene            complement(1368..>2643)
                     /locus_tag="PLU28_10350"
     CDS             complement(1368..>2643)
                     /locus_tag="PLU28_10350"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HOG35500.1"
                     /translation="QDMIKKLHDNNIKVILWQIPVLKILNDADEQHKKDLEYALEKKY
                     FVMKKDGTLYTIPPGRWFEGSYVIDLFNPQAREWFKEKRKYLIDELGVDGFKTDGGEH
                     LWERDTVVYGGISAAQARNLFTEKYFETEKEVVGQDKILFSRAGYTRSPSSTLFWVGD
                     EDSDIQALKANIVAGLNVSLCGNPFWGWDIAGFSGELPSIELYRRSLEIAVFTPIFQI
                     HSECNMGPYKSAERTPWNMADIFNDSSIIDYYRKIVSLRLSLIPYIAEQAEKSVNQKQ
                     PLTQPYNFTTDYFFGENIFVAPDLDGNNILEFILPPGQWIDLWEGKEYSGGLSYKYKC
                     KNHYSGVFIRKGTVLPINLPPSGKISDPGKGFEKNALLIGGFYEKEKPLLKNLNAGQI
                     ENVSFIGLFEESQKDGFMAKIKWMRIDEVDNE"
BASE COUNT          796 a          559 c          443 g          845 t
ORIGIN      
        1 caaacaggct tcccataatt gcaaagctga aggtatgccc ggtaaccatc agaaagacag
       61 ataaaacaga agaaaaactt atagtgcatg gcggtatagt tgacatgtcc ggaaaagaac
      121 ttataatact taccaccaaa gccgaacgtt ttgaagagat agatactttg aaaactaagg
      181 aaatgattga caagcttgaa aaagaagtga caacagctac acaagacagg gaaaaaataa
      241 agaacgaact tgaaaccaac aggatcaggt acaagtacgc gcagacagta aaaaaataat
      301 taaataaaaa atcagcagcc ttttctataa agctgctgat tttttatcaa ctattctgaa
      361 tatcaagaca tagattaata tactctgcat gtgcccataa aagtggagaa gctatatttc
      421 cccatctttt tacccatggt tcatacatat ccggtgctaa aagattctct gcaatctgtt
      481 cggcaagaaa accgtttgaa tcaaaatttg tatccagcca tcttttaatt tcaagagcct
      541 tttctttttc tccatttctg gcatagtaac ttccgagatt cgctgaaagt aaagtccagc
      601 ttccggctcc aaagtaagta tcgtttaaat aacgtttcag tccgcatcct tttttaagtg
      661 ttctttctat ttcgcttacc gtattgataa ccagttcatt tccgttgctg taaagtgcat
      721 aaggatatga gcaccataaa agacttgaat caacacagtc gttaccagtc gatttaacaa
      781 aacgtccgtt ttttgtatgc tgcgtcagaa ctttttgtag tataagttca tagcagttct
      841 tttgtccggt tgctatgctt tgggctttta gtcctgccgc aagcgaaaaa agtgtggaag
      901 tgtgaagata ttcaggacct tcttcccatg catcataaca tggaacttcc cagatttttt
      961 caatataatt tacaaccatt ttggaaacat cggcaggtac ttcataaccg cattcatggg
     1021 ctgcccataa aaatgttcca tatccgtcgc tttgcccatt aggccaatca tcattgtttg
     1081 ctgaaccatc gactctgtac ctcgtcggca aaaggtcttt atgtgttaat tcagacttat
     1141 ccttcagcag tactttttca atgtccggac gattgtttcg tatgactttt actgcccagt
     1201 caaaaaactt tcttgcgcaa atgctttcgc ctgctctgta cattgcccgt gcgataaatg
     1261 agctatccct gaaccagcag aatccatact gtgaaaagtt tggacttgcc acgtatgctc
     1321 cggaactgtc ctgatacttt ttaattatat ccaaagagtt caaaaaatta ttcattatct
     1381 acctcatcaa tcctcatcca ttttattttt gccataaaac cgtctttctg tgattcttca
     1441 aacaggccta taaaagaaac attttctatt tgtccggcat ttaaattttt tagaagaggt
     1501 ttttcttttt cataaaaacc gcctataaga agagcatttt tttcaaaacc ttttccagga
     1561 tcagatattt ttccgctagg aggaaggttt ataggaagta cagtaccttt tctgataaaa
     1621 acaccactgt aatgattttt acatttgtat ttgtagctga gaccacccga atactctttg
     1681 ccttcccaaa gatctatcca ctgacctgga ggaagaataa actctaaaat attatttccg
     1741 tcaagatcag gagcaacaaa aatgttttca ccaaaaaaat aatcagttgt aaaattatac
     1801 ggctgtgtta acggttgctt ttgatttacc gacttttctg cttgttcagc tatataagga
     1861 atcagagaca gtctaagcga aacaattttt ctatagtaat cgattataga gctgtcattg
     1921 aaaatatcag ccatgttcca tggtgttctc tctgcacttt tgtatggtcc catattacac
     1981 tctgaatgaa tctggaaaat aggtgtgaaa acagctattt caaggcttct gcgataaagc
     2041 tctatgctag gaagttctcc gctgaatcct gctatatccc atccccagaa aggatttcca
     2101 cagagagata catttaatcc tgccactatg tttgctttta aggcctgaat atcagaatcc
     2161 tcatctccta cccagaaaag cgtacttgaa ggagaacgtg tatatccggc acggctgaag
     2221 agtattttat cttgtccaac cacttctttt tctgtttcaa agtacttttc ggtaaagaga
     2281 tttcttgcct gggcagcact gattcctcca tatacaacgg tatcccgttc ccagagatgc
     2341 tctccgccgt ctgtcttgaa accgtctacc cccagttcat caataaggta cttccttttt
     2401 tccttaaacc attctcttgc ctgaggatta aaaaggtcaa tcacataact tccctcaaac
     2461 catcttccgg gaggtattgt gtatagagtg ccgtcttttt tcattacaaa gtattttttt
     2521 tcaagagcat actccaaatc ttttttatgc tgctcatcag catcattaag tattttgagc
     2581 accggaatct gccataaaat aacttttata ttgttatcat gaagtttttt aatcatatcc
     2641 tga
//
LOCUS       DAOSHR010000257         2625 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_37793_length_2625_cov_2.875097, whole genome shotgun sequence.
ACCESSION   DAOSHR010000257 DAOSHR010000000
VERSION     DAOSHR010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37793_length_2625_cov_2.875097"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1271
                     /locus_tag="PLU28_10355"
     CDS             <1..1271
                     /locus_tag="PLU28_10355"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOG35501.1"
                     /translation="NIKTVAERRENMLWFKVPPKIYFKYGSVETSLTELSDRKRAVII
                     TDKALFDQGYTDHVTQVLEQTGIDFEVFFDVEPDPSISTINKGLALINSFHPDLIIAM
                     GGGSPMDAAKIMWLMYEYPEVKFEGLALRFMDIRKRIYTFPVLGKKALMVAIPTTSGT
                     GSEVTPFAVVTDDKTGIKYPIADYALTPDMAIVDPQFVMNLPKKLTAYGGIDAVTHAI
                     EAYVSVFANEFTNGLALEALRLLFKYLPSSYKNGADDPKAREKVHYAATMAGMAFANS
                     FLGVCHSMAHKLGSSFHLPHGLANALLINETIRFNSTDVPFKQTAFPQYKYPLAKERY
                     AKIADYLNLHGNTPEEKVESLITAIQTLKDQLDIPSSISECGINTDEFYSKLDQMSEH
                     AFDDQCTSANPRYPLISEIKAMYIAAYSTK"
     gene            1413..1706
                     /locus_tag="PLU28_10360"
     CDS             1413..1706
                     /locus_tag="PLU28_10360"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOG35502.1"
                     /translation="MIELYVCMGSACYLKGSTDIIEILKEELQKNNLLGIIHMRGSFC
                     LGPCNQGVVIKVGNRFFKNVSPQNIKSLFENEIMPFIKNDEPSERAKDDDSYV"
     gene            1699..2193
                     /locus_tag="PLU28_10365"
     CDS             1699..2193
                     /locus_tag="PLU28_10365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOG35503.1"
                     /translation="MSKNVWEVLWDYDPNGLVALDENMQIKLVNPAFVKLFHLNDVDV
                     IGRHILEFFEDADDFYAILQGKSESIRKVHEYRNFGMILSEVTFRIENEGLLVKIFHD
                     VTEQERKEKEMKNLKIEIIEKVQNIVDKQMKVGQEIASILGETTAETKATLVKLLEIL
                     KKEG"
     gene            2226..>2625
                     /locus_tag="PLU28_10370"
     CDS             2226..>2625
                     /locus_tag="PLU28_10370"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOG35504.1"
                     /translation="MASAVVYRKSLNKFGEEVCGDNYQVSKTNDSKIVVLSDGLGSGI
                     KASILSILTTEIISTMMRMGVDIDEVVNTIAKTLPVCKVRGIAYSTFTIIQIYNNGNI
                     RIVNYDNPSPVIIKKGDIYYPDYKETLVDDK"
BASE COUNT          874 a          464 c          537 g          750 t
ORIGIN      
        1 ttaatataaa gacggttgct gaacggcgtg aaaatatgct ttggtttaaa gttcctccta
       61 aaatttattt caagtacggt tctgttgaaa cttcacttac agaactctct gacaggaaaa
      121 gagctgttat tattactgat aaggctttat tcgatcaggg gtatacagac cacgtcaccc
      181 aggtgctgga acaaaccgga attgactttg aagtgttttt tgatgttgag cctgatcctt
      241 ctatatctac cataaacaaa gggcttgctc ttatcaattc tttccatcct gatctaataa
      301 tcgctatggg aggaggctct cctatggacg ccgccaagat tatgtggctt atgtatgagt
      361 atccggaggt aaagtttgaa ggtctcgcac tgagatttat ggatatacga aaaagaatat
      421 ataccttccc ggttttagga aaaaaagcat taatggtcgc tataccaacc acttccggaa
      481 caggttccga agtcactcct ttcgcagttg ttaccgatga taaaacagga ataaaatacc
      541 ctatagccga ttatgcactt acacccgata tggctatagt tgacccgcag tttgtcatga
      601 atcttccaaa aaagcttact gcatatggcg gaatagacgc tgtaactcac gctatagaag
      661 cttatgtttc tgtatttgca aatgaattta caaacggttt ggcattggaa gctttgagac
      721 ttcttttcaa atatctgccc agttcgtata aaaacggagc agatgaccct aaagcaagag
      781 aaaaggttca ttatgcagcc actatggcag gaatggcttt tgcaaactct tttctcggag
      841 tgtgtcattc tatggcacat aaacttggct cttcatttca tcttcctcat ggacttgcta
      901 atgctctgct tataaatgag actatccgtt tcaactcaac agatgttcct tttaagcaga
      961 ctgcattccc acagtataag taccctcttg caaaggaaag gtacgcaaaa attgccgatt
     1021 atcttaatct gcacgggaat accccggaag aaaaagttga aagccttata actgccattc
     1081 aaactctcaa agatcagttg gatattccaa gctccataag cgagtgcggc ataaacaccg
     1141 atgagttcta ctcaaaactg gatcagatgt ctgaacatgc ctttgatgat cagtgtactt
     1201 ctgccaaccc aagatatcct cttatttctg aaataaaagc aatgtacata gcggcttatt
     1261 ctacaaaata aaagtatttt acctttcatt tatcaacgaa agagaagtat ttggaaaagt
     1321 cagttaacat atatgaagta taatatcatt gtattcaaag aatgtgagtt ttttcttttt
     1381 tcgggaaaca aatgatgaaa agaggtggaa atatgattga actgtacgta tgtatgggaa
     1441 gtgcatgtta cctcaaagga tcgactgaca ttatagagat acttaaggaa gagcttcaga
     1501 agaataatct tcttggtatc atacacatga gaggatcttt ctgtctgggt ccatgcaatc
     1561 aaggtgttgt cataaaggta ggaaacaggt tcttcaaaaa cgtaagtcct cagaatatta
     1621 aaagtctttt tgaaaatgag atcatgccgt tcataaagaa tgatgagcct tcagaaaggg
     1681 caaaggatga tgattcatat gtctaaaaat gtctgggaag tcctttggga ttatgatccg
     1741 aacggtctgg tcgcactgga tgaaaacatg cagataaaac tggtaaatcc tgcttttgtt
     1801 aaactttttc atcttaacga tgtagacgtt ataggacgcc atatacttga gtttttcgaa
     1861 gatgcagatg acttttatgc catactccag ggaaaaagcg aatccataag aaaggtgcat
     1921 gagtacagaa atttcggaat gatactttct gaagtaactt tcaggataga aaacgaaggt
     1981 cttcttgtaa aaattttcca tgatgtcact gaacaggaaa gaaaagaaaa agagatgaaa
     2041 aatcttaaaa tagagatcat agaaaaggta cagaatattg ttgacaaaca gatgaaggta
     2101 ggccaggaaa tagcttccat tcttggagag actacggctg aaactaaagc tactctggta
     2161 aaactgcttg aaattctcaa aaaggaaggg tagtaagatc acctaaaata ggctggtggt
     2221 gaaatatggc atctgcggta gtttatagaa aaagtttaaa taaatttggt gaggaagtct
     2281 gcggtgacaa ttatcaggtt tcgaaaacca atgattcaaa gatcgtggtt ctgagcgatg
     2341 gcttgggaag cggtataaaa gccagtatcc tatctattct tactacagag ataataagta
     2401 ccatgatgag aatgggtgtt gatatagacg aagttgtgaa taccatagcc aagactctgc
     2461 ccgtatgtaa ggtcagaggt attgcttact caacctttac cataatccag atatacaaca
     2521 acggaaacat aaggatagtc aattacgata acccatctcc tgttataatc aaaaaaggag
     2581 atatatacta cccggactat aaagaaactt tggttgacga caaaa
//
LOCUS       DAOSHR010000258         2583 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_38704_length_2583_cov_4.236155, whole genome shotgun sequence.
ACCESSION   DAOSHR010000258 DAOSHR010000000
VERSION     DAOSHR010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38704_length_2583_cov_4.236155"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="PLU28_10375"
     CDS             <1..358
                     /locus_tag="PLU28_10375"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HOG35505.1"
                     /translation="IKPQEFKVYSGNDDQTVPVMALGGDGVISVFSNICPVEMKWIAD
                     SILNNDMQTARNINNKYLELMNLLFVDVNPVPVKYAASLMNFTSDYVRLPLVPLSDNH
                     RKVLSDKMKELNIVCC"
     gene            349..1023
                     /locus_tag="PLU28_10380"
     CDS             349..1023
                     /locus_tag="PLU28_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HOG35506.1"
                     /translation="MLLKYGLIGSSGKMGKEVIEFFTENGHEMVYSMDINGETMTAEP
                     DVLIDFSLAGAFEKTLETAYRLKKPFIMGTTGLTKQNMKDLKKLSKQIPVIYSTNFSI
                     GIQLMICMAEFMKKNTDEWDIGISEIHHRFKKDKPSGTANTICQALGGGIEINSQRLG
                     NIAGEHTVSFASLGEVITITHRALSRRTFAEGVLQSAYFLIGKPSGMYTFRQVIVGEG
                     NGNGQL"
     gene            1004..1714
                     /gene="dapD"
                     /locus_tag="PLU28_10385"
     CDS             1004..1714
                     /gene="dapD"
                     /locus_tag="PLU28_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012009788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HOG35507.1"
                     /translation="MEMDSYEIIKYIANSEKKSPVQVYIKGELEDIDYGKYDIQFFGS
                     EEWGVIFADYKKYLQFRAENHEKITGEKIISDRVNSAVELADILKYKARIEPGSIIRE
                     MVDIGENAVIMMGAVLNIGCSIGKNTMIDMNVVVGGRAVIGENCHIGAGTVVAGVIEP
                     PSAQPVVIGNNVLVGANAIIIEGVKVGDGAVVAAGSAVINDVPPNTLVAGVPARFIKQ
                     VDDKTRGKSQILQELRKL"
     gene            1737..>2583
                     /locus_tag="PLU28_10390"
     CDS             1737..>2583
                     /locus_tag="PLU28_10390"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOG35508.1"
                     /translation="MTVVQKYGGSSVADTEKIKRVAMKIKDRTVKGDKIVVVVSAMGK
                     TTDNLIKLAGEVSSVPDPREMDMLLTTGEQISVALLSMSLKQAGVRSISINAFQAGIM
                     TTSDFNCARIKNFDVQKIKKLLMDYDVVVVTGFQGITDNGDLTTLGRGGSDTSAVALA
                     AALEFNCEIYSDVKGIYTVDPKLHPSAKKLKSISYDEILEMANSGAKVLHDRSVEIAK
                     KYGVVLYCAGTFSEEEGTYVMQTNIEDPVVSGLSVMENQTRVVISNLPLDYKLVHKIF
                     ERVAKD"
BASE COUNT          860 a          431 c          626 g          666 t
ORIGIN      
        1 aataaaacct caagaattca aagtatacag cggaaatgac gatcagacag ttcctgtgat
       61 ggcactggga ggagacggtg ttatttcagt cttttcaaac atatgtcctg ttgaaatgaa
      121 atggatagca gattcaattc ttaacaacga tatgcagacg gcacgtaata taaataataa
      181 gtacctggag cttatgaacc tgttgtttgt ggacgtaaat cctgtgcctg taaagtatgc
      241 tgcaagtctc atgaacttta cgagtgatta cgtaagactt ccgcttgtac ccttaagcga
      301 taatcaccga aaggtacttt cagataaaat gaaggagttg aatatagtat gttgttgaag
      361 tacggactga taggaagcag cggaaagatg ggaaaagaag ttatagaatt ttttactgaa
      421 aatggacatg agatggttta ctcaatggat ataaacggtg aaaccatgac tgcggaaccg
      481 gatgttctta tagatttttc tcttgcaggg gcttttgaaa aaactcttga gaccgcatac
      541 agattaaaaa aaccttttat aatgggaacc acaggtctta ccaaacagaa catgaaagat
      601 cttaaaaaac tttcaaagca gattccggtg atatacagta caaacttttc cataggtata
      661 cagcttatga tatgtatggc tgagtttatg aaaaaaaaca cggacgaatg ggatattggt
      721 atatcagaaa ttcatcacag atttaaaaaa gacaagccat ctggaaccgc taataccata
      781 tgtcaggcac taggaggcgg tattgagata aactcccaga ggcttggtaa tatagcagga
      841 gagcataccg taagctttgc aagtcttgga gaggtcataa ccataactca tagggcgctt
      901 tcacgcagaa catttgctga aggggttctc cagagtgcat actttttaat cggtaaacct
      961 tcgggcatgt atactttcag acaggtaata gtcggagagg ggaatggaaa tggacagtta
     1021 tgagattata aagtatattg caaactccga aaaaaaaagt cccgttcagg tttacataaa
     1081 aggcgaactt gaagatattg attatggcaa gtacgatatt caattttttg gcagtgaaga
     1141 gtggggagtt atattcgccg attataaaaa gtatctacaa ttccgcgcgg aaaatcatga
     1201 aaagattacc ggcgaaaaaa tcatatccga cagagtgaac tcagctgttg aacttgcaga
     1261 tattttaaag tacaaagcaa ggatagaacc cggttccatt atacgagaga tggttgatat
     1321 aggtgaaaat gccgttataa tgatgggggc ggttctgaat ataggctgca gcataggaaa
     1381 aaacaccatg atagatatga atgtggttgt tggaggcagg gctgtaatcg gtgaaaactg
     1441 ccatataggt gcaggaaccg tagttgccgg agtcatagaa cccccgagtg ctcagcctgt
     1501 ggttataggc aataatgttc tggtgggagc aaacgccata ataattgagg gagtgaaggt
     1561 gggcgacgga gcggtagttg ctgccgggtc ggctgtgata aacgatgttc ctcccaatac
     1621 tcttgttgca ggagttccag caagatttat aaaacaggtg gatgataaaa caaggggaaa
     1681 gtcccagatt ctgcaggaat tgagaaagct gtgagattag aaggaggaca gcaaagatga
     1741 ctgtggttca aaaatacggc ggctcttcgg tagctgatac cgaaaaaata aaaagagttg
     1801 ccatgaaaat aaaagatagg actgtcaaag gcgacaagat tgtagttgta gtgtctgcaa
     1861 tgggtaagac cacggataat cttataaagc ttgcaggaga agtatcctct gttccagatc
     1921 cacgtgaaat ggatatgctt cttacaacag gcgaacagat atcggttgca cttctttcca
     1981 tgtctttaaa gcaagccggg gtccgttcca tatcgataaa tgcctttcag gcaggtataa
     2041 tgaccacatc tgatttcaac tgtgcaagaa taaagaactt tgatgtgcaa aagataaaaa
     2101 aacttctcat ggactatgat gtggttgtag taacaggttt tcagggtata acagataatg
     2161 gtgatctgac cactctgggc agaggaggtt cggatacttc tgcggtggcg cttgctgcag
     2221 cactggagtt caactgtgaa atatactctg atgtaaaagg catatataca gttgatccaa
     2281 aacttcaccc atcagccaaa aaattaaaaa gcatttctta tgatgagata cttgaaatgg
     2341 ctaactccgg tgccaaagta cttcatgaca gatcagtcga gatagctaaa aaatatgggg
     2401 tggtgcttta ctgcgccgga acgttcagtg aagaggaggg aacatacgta atgcagacta
     2461 acatagaaga tcctgtagtc agcggtctga gtgtaatgga aaatcagacc agggtcgtga
     2521 tttcaaacct gccgcttgat tataagctgg tacataaaat ttttgaaaga gtcgcaaaag
     2581 atg
//
LOCUS       DAOSHR010000259         2566 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_39074_length_2566_cov_4.050179, whole genome shotgun sequence.
ACCESSION   DAOSHR010000259 DAOSHR010000000
VERSION     DAOSHR010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39074_length_2566_cov_4.050179"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..691)
                     /locus_tag="PLU28_10395"
     CDS             complement(2..691)
                     /locus_tag="PLU28_10395"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake domain-containing protein"
                     /protein_id="HOG35509.1"
                     /translation="MSDFIEKVYASSSISANLPMEIEVEEKMHRGRYKSVIHDYNAQT
                     GVAHVGIPIYKSAYIKLPPDLPIKIRVYSKMAVYLFNSSVISVGEEGNIKYLSINVPE
                     IVFKVQRRQYVRVPIAVEGFFYLKQEYDTLDVVPKYKFISKDFSAGGMSVVTKKDVPA
                     GTEILINFKVWDELSLDNFLSETVRLIGKTSFGESIYGVRFKNLDPVMEKDLVKFVFR
                     YEIESTKKNRR"
     gene            complement(704..1288)
                     /locus_tag="PLU28_10400"
     CDS             complement(704..1288)
                     /locus_tag="PLU28_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35510.1"
                     /translation="MDKSFLFLALKMALYRGNSIKITLKGVTETVKIIDYHESKIILT
                     LPRQEVQLEDIVNIELFYNGNYKILGRCTAVDEKYITLEVKNDVQYTTKRKNFRIPVF
                     FPVIIDGQFRGTLVDFNVDRLVAVVAYSNLKLRGESIKLKFMGKNDIMISGKIISKRD
                     EIFNLERYIIQFEESEENSKVQSVFQTIVDYFKT"
     gene            complement(1292..2140)
                     /locus_tag="PLU28_10405"
     CDS             complement(1292..2140)
                     /locus_tag="PLU28_10405"
                     /inference="COORDINATES: protein motif:HMM:NF012369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HOG35511.1"
                     /translation="MTHKLADQAERLRNDFSAKETKIICISGGKGGVGKSLISVNIAV
                     EIAKRGKSVLIFDSDAGFANASILLGKTLKNTLSDYINNNLPLEKCIQKTEYGVNVIS
                     TGFDFKDWKIYQSSFSPKMVEDLFLLSKDNDYVIVDIGAGYSETIKPFYLAANDILMV
                     TVPEPTAIVNAYTLIKALSIIGVTADLEIILNMVRDRSEVNSVESVLRKTVKSFLNRD
                     IKEFYTIFYDEQIHESVKRQTPLVYYKENAKFSKSIQSIVEGIDTNLAPKKYGFKERL
                     LSIFVR"
     gene            complement(2137..>2566)
                     /locus_tag="PLU28_10410"
     CDS             complement(2137..>2566)
                     /locus_tag="PLU28_10410"
                     /inference="COORDINATES: protein motif:HMM:NF012662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35512.1"
                     /translation="IMGVPAFICYTPGDLRSTLQTLLGYDAVLIDTAGRSHKNDLHLG
                     ELKVYIDTANPEERVLIMAANTRTQDLINIVENFGIAKPSSLIFTKIDETSFYGQIFS
                     VAKKTDLAISYITTGQKVPDDINVIEPEYFARKASEEVFR"
BASE COUNT          753 a          545 c          396 g          872 t
ORIGIN      
        1 attaccttct gtttttcttg gtggattcta tttcatacct gaaaacaaac ttaaccaaat
       61 ctttttccat gacaggatca agatttttga accttactcc gtagatgctc tctccaaaag
      121 atgttttccc tataagtctc actgtttcgg aaagaaaatt atccaacgaa agttcatccc
      181 ataccttaaa atttataaga atttcggttc cagcaggtac atcttttttt gtaaccacag
      241 acattccccc agcactgaaa tcttttgata taaacttata ttttggcaca acatctaatg
      301 tgtcatattc ctgttttaag taaaaaaagc cttccactgc aatcggaact cttacatact
      361 gccttctctg aactttgaaa acaatctcgg gaacatttat ggaaagatac tttatattcc
      421 cctcttcccc tacagaaata accgagctgt taaaaagata tacagccatc tttgaataca
      481 ctcttatctt tatcggaaga tcaggcggaa gttttatata agcactttta tatattggaa
      541 ttccaacgtg cgctacacct gtctgagcat tataatcatg aattacactt ttatacctgc
      601 ccctgtgcat cttttcttct acttcgattt ccatagggag attagctgaa atactcgaac
      661 ttgcatatac tttttctata aaatcagaca taccatctcc cccttaagtc ttaaaataat
      721 cgacaatcgt ctgaaaaacg gactgtactt tagagttttc ttcggactcc tcaaactgaa
      781 ttatatatct ctcaagatta aaaatctcat ctcttttact tattattttc cctgatatca
      841 ttatatcatt tttgcccata aattttagtt taatactttc ccctctcagt ttaagatttg
      901 aataggccac aaccgccaca agcctgtcta cgttgaaatc aacaagagtg cctctgaact
      961 gtccgtctat tattacaggg aaaaatacgg gaatacggaa attctttctt ttagttgtat
     1021 actggacatc attcttgact tccaatgtaa tatacttttc atcgactgct gtacatcttc
     1081 cgagtatttt atagttgccg ttgtaaaaca actctatgtt tactatatct tctaactgta
     1141 cttcctgtct gggaagagta agaataattt ttgactcatg ataatctata atttttacgg
     1201 tctcagtaac tccttttaaa gtgattttta tactgtttcc cctgtaaaga gccattttaa
     1261 gcgccagaaa aaggaagctt ttatccattt ctcaccttac gaaaatagag agcaggcgtt
     1321 ctttaaagcc atattttttg ggtgcaagat tggtatctat gccttcgact atactctgta
     1381 tagactttga gaacttggca ttctctttat aatagaccaa aggagtctgc cgcttgacgc
     1441 tttcgtgaat ctgttcgtca tagaatatag tataaaactc ttttatatct ctgttcaaaa
     1501 aagatttaac cgtttttctc agtacggatt caaccgagtt tacttccgac ctgtccctta
     1561 ccatattcag tattatttca agatcggctg tgactcctat tattgaaagg gcttttatca
     1621 gagtataagc atttactata gcggtaggtt ccgggacagt caccatgagt atgtcattag
     1681 ctgcaagata aaaaggtttt atggtttctg aatagcctgc cccgatatcg acaattacat
     1741 aatcgttatc tttggaaagc aggaaaagat cttcaaccat cttgggtgaa aagctactct
     1801 gataaatttt ccaatcttta aaatcaaaac cagtgcttat tacatttact ccgtactctg
     1861 tcttctgaat acacttttca agcggaagat tgttgtttat gtaatcagaa agcgtgtttt
     1921 tgagagtttt gcccaatagt atagaagcat ttgcaaaacc ggcatcagaa tcgaatatca
     1981 gaacgctttt ccctctcttg gcaatctcaa cagcaatgtt tacagaaatg agagacttac
     2041 caacaccgcc ttttccacct gaaatacata taatcttggt ctccttggca gagaaatcat
     2101 ttctcaatct ttcagcttga tccgcaagct tatgagtcat cggaatacct cctctgatgc
     2161 cttcctggca aagtattctg gttcaattac attaatatca tcaggtactt tctgtcctgt
     2221 ggttatataa ctaattgcga gatctgtttt tttcgcaacc gagaaaatct gaccataaaa
     2281 agatgtttca tcaattttgg taaatatgag cgaacttggt tttgcaattc caaaattttc
     2341 tacaatattt atcaaatcct gcgtacgtgt atttgctgcc ataataagca ccctttcctc
     2401 gggattcgct gtatctatat ataccttcag ctctccgaga tgaaggtcat ttttatgact
     2461 gcgccccgca gtgtctataa gcacggcatc ataccccaaa agagtctgaa gcgtgctcct
     2521 aaggtctcca ggagtatagc atataaaagc aggcactccc attata
//
LOCUS       DAOSHR010000260         2544 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_39590_length_2544_cov_4.277622, whole genome shotgun sequence.
ACCESSION   DAOSHR010000260 DAOSHR010000000
VERSION     DAOSHR010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39590_length_2544_cov_4.277622"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="PLU28_10415"
     CDS             complement(<1..650)
                     /locus_tag="PLU28_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35513.1"
                     /translation="MTFKDFKDNIRNFLKDFFSYKSGVVGLVLLLLFLVMLAFGGLLV
                     GFPQASSRWRDITYWEDNPVSAPPAWTNWFSKQKSAKHEVIGSSNMEEKSIGSMKMIT
                     YSFDYDYSYDLAPNDIVVKAEGSGSPIVKISVQRPDGNTINLYSSSTGFKSDGTLRVS
                     LNGDAKANVYDFITDFESGEVLSNFNMNDLIPCNVIFSQAEENIVEKANALKGKYI"
     gene            complement(647..1690)
                     /locus_tag="PLU28_10420"
     CDS             complement(647..1690)
                     /locus_tag="PLU28_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG35514.1"
                     /translation="MYWKYALKRILYGLIIYVILIFIFSALFNTTVEQTARTEIYEKM
                     SSELSRVTQNMSPEQVEVYRTQYLERKFKEYNLDKPLFERIIWRTYNSVTFQYGNSTR
                     IKTSQGEKEVIKIIGETIPKTLLLFLIAFGIEIIIGILVGIKKAQKAGGMMDKITSVA
                     TMIVYGMPSWWLGMILIMFFAYSIKLFPSGGVYSTPPMVGAMKIIDIAYHLVLPVFTL
                     VILNFWGTAFVTRNIVLGTLQEDFVMSARARGIPEKKVLFGHAMRTAAPPIITIAVLT
                     LLSSVSGSLLFEGIFSWPGMGNLYWTAIQMNDIPVLMGDLSVTTAIYMAGLVFLDLIY
                     GFLDPRIKVGGKA"
     gene            complement(1748..>2544)
                     /locus_tag="PLU28_10425"
     CDS             complement(1748..>2544)
                     /locus_tag="PLU28_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35515.1"
                     /translation="DYNFPADKNRVAQFGIPALSVTASGHYVGTSWEIIEALDKIITE
                     SNKSGTKYSITREEAFTEVDVLMSKCVEDIRAKLVEMKNQKWVPVYIRNFMTADEAVK
                     CYDLAIKFIDEHKHALIANGPFILNRYDAETDFVELIANRNFAYSAEEIIKAFSTNFA
                     RIDKIEIPEMSEKGKDITVKAKISSISYPANKVSMLEKGSAKAILITSDKEMTFEMKM
                     NKAGNFEGTIPASATKNLAEGTYTVVVVAEVQNGSPAAGSQLVIIY"
BASE COUNT          752 a          532 c          431 g          829 t
ORIGIN      
        1 aaaatgtact ttccttttaa tgcattggct ttttcaacta tgttttcttc tgcttgagaa
       61 aaaatcacat tacatggaat gaggtcgttc atgttaaagt ttgaaagaac ttctccactc
      121 tcaaaatcag ttataaaatc atagacattt gcttttgcat ctccgttaag ggatactcta
      181 agcgtaccgt cgctcttgaa tccggtcgat gaactgtaaa gatttattgt attaccgtcg
      241 ggtctttgaa ccgatatctt tactatagga gaaccgcttc cctcagcttt gacaactatg
      301 tcgttggggg caagatcgta gctgtaatca tagtcaaagc tataggttat cattttcata
      361 gagccgatac ttttttcttc catgtttgat gaaccaataa cctcatgttt agccgatttt
      421 tgttttgaaa accagttggt ccacgcagga ggtgcactta caggattatc ttcccaatag
      481 gttatgtctc tccagcggga gcttgcctga ggaaaaccga ctagaagacc tccaaatgca
      541 agcattacta aaaacaggag taaaagaaca agccctacta ctccggattt ataactgaag
      601 aaatccttta aaaaatttct tatattatct ttaaaatctt tgaatgtcat gctttaccac
      661 caactttgat tctcggatcc aaaaatccgt agattaaatc aaggaaaacc agacccgcca
      721 tatatatagc ggtagttacc gaaagatctc ccatcaatac cgggatatcg ttcatctgta
      781 tagctgtcca gtaaagattt cccataccag gccagctaaa gataccttca aaaagcaaac
      841 ttccgcttac agatgacaac aaggtaagaa cagctatggt aataatagga ggtgccgcag
      901 ttctcatggc atgaccaaaa agaactttct tttcaggtat accccttgct ctggctgaca
      961 ttacaaaatc ctcctgcaac gttccgagca caatatttct tgtgacaaaa gcagttcccc
     1021 agaaatttaa aataacaagt gtgaataccg gcaggacaag atggtaggcg atatcaataa
     1081 ttttcatggc tccaaccata ggcggagtag aataaactcc tcctgaaggg aaaagcttta
     1141 tactgtacgc aaaaaacatt ataagaatca ttcccagcca ccagctcggc atcccgtata
     1201 ctatcatcgt tgcaacggaa gttattttgt ccatcatacc tccggctttc tgggcctttt
     1261 ttattccgac cagtatacct attattatct ctatgccaaa agctataaga aataaaagca
     1321 gagttttagg aatagtttca cctattatct ttattacttc tttctctcct tgagatgttt
     1381 ttattctagt agagttgccg tattgaaagg taactgaatt atatgttcgc catataattc
     1441 tttcaaaaag aggtttgtca agattgtact ctttaaactt tctttcaaga tactgtgtcc
     1501 tgtaaacttc aacttgttca ggagacatgt tttgagtcac ccttgaaagt tccgaactca
     1561 ttttttcata tatttcagtt cttgccgtct gttcaacagt tgtattgaag agggcggaaa
     1621 aaataaaaat aagtatcaca tatataatta atccgtacaa aattcttttc aaagcatatt
     1681 tccaatacat atactcacct cagatgtcat aaaaagtagt acccgttgcc gggtactaca
     1741 gaagattcta gtaaataata accaactgtg aacctgctgc aggcgaaccg ttctgaactt
     1801 ctgcaaccac aaccaccgta taagtacctt ctgcaaggtt ctttgttgca ctggcaggta
     1861 tcgttccttc aaaatttcca gccttgttca tcttcatctc aaatgtcatt tccttatctg
     1921 aagttattag aatagccttt gctgaacctt tttcaagcat tgatactttg ttggcaggat
     1981 aggatatcga tgaaattttt gcttttacag ttatatcctt acctttttct gacatctcag
     2041 gaatttctat tttgtcaatt cttgcaaaat tagtgctgaa tgcttttatt atttcttctg
     2101 cactgtatgc aaagtttctg ttggcaataa gttctacaaa atctgtttcg gcatcgtatc
     2161 tgttaagtat gaaaggaccg tttgcaatga gagcatgttt gtgctcatca ataaacttga
     2221 ttgcaagatc ataacactta acagcttcgt ctgcagtcat gaagtttctt atataaaccg
     2281 gaacccactt ttgattcttc atttcaacca gcttggctct tatgtcttca acacatttgg
     2341 acatcaacac gtcaacttcc gtaaatgctt cttctcttgt tatggagtac tttgtacctg
     2401 atttgttaga ctctgtaata attttatcca gagcttctat tatttcccat gatgttccta
     2461 cataatgtcc ggatgctgta accgaaagag ccggaatacc gaactgtgca actctgttct
     2521 tatcggccgg gaagttatag tcat
//
LOCUS       DAOSHR010000261         2543 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_39617_length_2543_cov_4.481913, whole genome shotgun sequence.
ACCESSION   DAOSHR010000261 DAOSHR010000000
VERSION     DAOSHR010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39617_length_2543_cov_4.481913"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            91..1161
                     /gene="prfA"
                     /locus_tag="PLU28_10430"
     CDS             91..1161
                     /gene="prfA"
                     /locus_tag="PLU28_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOG35516.1"
                     /translation="MDFLQFKESLLEKLGQVEQDLSNPEITSIPENLQKYGQEHTRLT
                     ELKELFVSYEKALGDLHFLDEMSAQKEISEEEYVPMKAELELSIKGYEFDILKKLVPA
                     DELDNRNIIIEVRAGAGGDEAGLFGGELMRMYMRYAERKNWKYELLDIAENGVHGIKT
                     AIFKIRGKEVFGRLKYEIGVHRVQRVPETEASGRIHTSTATVAVLPEVTDIDVVIDPK
                     DLRIDTYRAGGAGGQYVNKTESAVRVTHIPSGIVVACQNERDQHQNKEVAMNILRAKL
                     FESKLMEQQKQMTDARRSQIGSGDRSEKIRTYNFPQNRVTDHRIGFTTYRINELIDGD
                     LDELIDKLIEFDLNERLENIKI"
     gene            1181..1609
                     /locus_tag="PLU28_10435"
     CDS             1181..1609
                     /locus_tag="PLU28_10435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35517.1"
                     /translation="MFKNLSVFYSSFFITGILLGFIEIMQSDHLSLLSIVFYFLAQMT
                     LIVGLYLFERKQGKEKSFAPNFSLVSIVLESMLFMVLFDMNNMSLWLFGIINMVFVYF
                     SCVLSYNFLRSLDLDIAFTFLLKRFMTLNFFSELDNLKMK"
     gene            1650..>2543
                     /locus_tag="PLU28_10440"
     CDS             1650..>2543
                     /locus_tag="PLU28_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HOG35518.1"
                     /translation="MVDITLPDGSVKNFEMGVTPAEIAKSISEGLFRKAFGAMVNGEM
                     RDLYSPITQNSSVAIITEKDPQAASIFRHTMAHIMAQAVMHVFGKENVKFAIGPVIDN
                     GFYYDFDIPSHKITEEDFSKIEEEMKNIIRQDIKISRKDLSKKEAAELFADQPYKLEL
                     ITDIVDDTVSVYTQGDFSDLCRGPHLPSTGMVKYAKILSVSGAYWRGDEKNKMLQRIY
                     ATSFVKKDELDAYVTMIEEAKKRDHRKLGPALGLFMIDTDIAPGMAFFLPRGTIALNE
                     MRKLSREIHKKYEYVEVETPQI"
BASE COUNT          822 a          435 c          573 g          713 t
ORIGIN      
        1 ataagttttt gaaaacaatg atatgttata attatactta aatcagaata gtaactgtca
       61 gaatctttat tttgaaccgg agggataatc ttggattttt tgcagtttaa agaaagcctt
      121 cttgaaaaac ttggacaggt tgaacaggat ctcagtaatc ctgaaataac ttctatacct
      181 gagaatcttc agaaatacgg tcaggagcac acacgtctta ccgaactgaa agagcttttc
      241 gtatcttatg aaaaagctct tggagatctt cattttcttg acgagatgag cgcacaaaaa
      301 gaaataagcg aagaagagta cgttccaatg aaagcagaat tagaactttc cataaaaggc
      361 tatgagttcg atattctaaa aaaattagtg cctgccgatg agcttgataa cagaaatatc
      421 atcatagaag tcagagcagg tgccggcggt gatgaagccg ggcttttcgg cggcgagctt
      481 atgagaatgt atatgcgtta tgccgaaagg aagaattgga agtatgagct gcttgacatt
      541 gctgaaaacg gggttcacgg aattaaaact gccatattca aaatacgcgg taaggaggtt
      601 ttcggaagac tgaaatacga aattggggtt cacagagtac agcgtgttcc agaaactgaa
      661 gccagcggaa gaatccacac ttcgaccgca acggtggcag tactgccgga ggttacggat
      721 attgacgttg taattgatcc gaaagacctg agaatcgata cttaccgtgc cggaggagca
      781 ggcggccagt atgtcaacaa aaccgaatcg gctgtcaggg ttactcatat accaagcgga
      841 atagttgttg catgtcagaa cgaacgtgac caacatcaaa acaaggaagt tgccatgaac
      901 atactaagag cgaagctttt tgaaagcaag ttgatggaac agcagaaaca gatgactgat
      961 gcacggaggt cacagatcgg aagcggtgac agaagtgaaa agattagaac atacaacttt
     1021 cctcagaaca gggttacgga tcacaggata ggttttacga cctataggat aaatgaactt
     1081 atagatggtg atcttgatga acttatagat aagcttattg aatttgatct caatgaaagg
     1141 cttgagaata taaaaatata agttttcggg aggaaggtgc atgtttaaga atttaagcgt
     1201 tttttacagc agttttttta taacaggcat tttacttggt tttatagaga taatgcagag
     1261 cgatcatctg tccttgcttt ctatcgtttt ttattttctt gctcagatga cccttatagt
     1321 cggtctttat ctttttgagc gtaagcaagg aaaagaaaag agttttgcgc ctaatttttc
     1381 gcttgtttca atcgtgctgg aatcaatgct tttcatggtt ctttttgaca tgaacaacat
     1441 gagcttatgg ctatttggca taataaacat ggttttcgtg tacttcagct gtgtcctttc
     1501 ttataacttt ctgagatctt tggatcttga tatagctttt acttttttat tgaaaaggtt
     1561 tatgacgctt aactttttca gcgagttaga caatctaaaa atgaagtaac aaaaggtatg
     1621 gtttcatata aaaaacgagg tgattttgta tggtagacat tacacttcct gacggtagcg
     1681 taaagaactt tgagatggga gtaaccccgg cggaaatagc caaatccatc tccgaagggt
     1741 tatttagaaa ggcttttggt gctatggtaa atggagagat gagggatctt tactctccta
     1801 taactcagaa cagctcggtg gctattatta ccgaaaaaga tccgcaggca gcatctattt
     1861 tcagacatac tatggcacac atcatggcac aggcagtaat gcacgttttc gggaaagaaa
     1921 acgttaagtt cgccataggc cctgttatag acaacggctt ttattatgac tttgatattc
     1981 cttcccataa gataaccgaa gaggattttt ctaagattga agaagaaatg aagaatatca
     2041 tcaggcagga tataaagatt tcaagaaagg acctatccaa aaaagaagcg gcagagcttt
     2101 ttgcagatca gccgtataag cttgaactaa taacagatat tgtcgatgac acagtttcgg
     2161 tatatactca gggagatttt tccgatcttt gcagaggacc tcatcttccc tcgaccggaa
     2221 tggtcaagta cgctaagatt ctttctgttt ccggggcata ctggagaggc gatgaaaaga
     2281 acaaaatgct ccagagaata tacgctacat cgtttgtaaa gaaagacgag ctcgatgctt
     2341 atgttaccat gatcgaagaa gctaaaaaaa gagaccacag aaagcttgga cctgctctgg
     2401 gattgtttat gattgatacg gacatagctc ccggaatggc tttctttctg cccagaggca
     2461 ctatagcgct taatgaaatg agaaagttat cacgggaaat acacaaaaag tatgaatatg
     2521 tagaagttga aactcctcag ata
//
LOCUS       DAOSHR010000262         2533 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_39850_length_2533_cov_4.225182, whole genome shotgun sequence.
ACCESSION   DAOSHR010000262 DAOSHR010000000
VERSION     DAOSHR010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39850_length_2533_cov_4.225182"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1265
                     /gene="cysS"
                     /locus_tag="PLU28_10445"
     CDS             <1..1265
                     /gene="cysS"
                     /locus_tag="PLU28_10445"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOG35519.1"
                     /translation="FDAFRRFLEYVGYKVTMVQNFTDIDDKIIIKAVKEGVPFNEVSE
                     RYIVEYMKDALALRIRTANFHPKTTNYVHEIIKFINELIDKGFAYESAGDVYFDVRKF
                     EQYGMLSHRNPDDMRSGSRIEVSDIKTDPLDFALWKKAKENEPSWESPWGKGRPGWHI
                     ECSVMATELLGESFDIHAGGNDLIFPHHENERAQSMAHSGNDFANYWMHNGMIKVAGD
                     KMSKSIGNIWLVRDVVSKYGADTVKVFVLSKHYRVPLDFSEEGLQAQKKSVSRVQQAL
                     KDAEDFFAGTVPYVKNTEYMKSKIETFKQALSDDFNTSKAVALIFDLSAELNKALSEK
                     NETHVIENYHLIRNEFGPVLGIFEIQETQQKSSTDDTDKVMQVLIQLRNQARTDKNYT
                     LSDSIRDGLASIGIQLKDTPEGTKYIIQ"
     gene            complement(1290..2420)
                     /gene="tgt"
                     /locus_tag="PLU28_10450"
     CDS             complement(1290..2420)
                     /gene="tgt"
                     /locus_tag="PLU28_10450"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOG35520.1"
                     /translation="MDDVSPIKYELLHIDRYTNARRGRIHTKHGIIETPVFMPVGTNA
                     TVKALTGQSLEDVGSEIILGNAFHLFLRPGLDVLDSFGGLHSFMNWKKPILTDSGGFQ
                     VFSLKDRIIDEQGVSFRSPLDGSKLKITPEISMKIQQSIGSDIAMAFDECITAYADHD
                     YVSSSIDRTFRWAKRSFESHSRKDQALFGIVQGALFEDLRQRSLEQITSVPFDGYALG
                     GLAVGEKYQESEKILQSFGTQLPADKPRYIMGIGSPTMMVLSVENGMDMFDCVLPTRM
                     GRHGTALTYNGRINLKASKCAYIEKPIDENCSCYTCRNHSQGYIHHLFKRDEILGKML
                     LSIHNLHFNISLVKDMRKAIEEDRFLQFKKQFFEVSGYPLKN"
BASE COUNT          779 a          490 c          550 g          714 t
ORIGIN      
        1 tttttgatgc tttcaggagg tttcttgaat atgtaggcta taaagttact atggttcaga
       61 actttaccga tattgacgat aagataataa taaaagcagt taaagaagga gttcctttta
      121 acgaggtatc cgaaagatat attgttgagt atatgaaaga tgccctggct ctgagaataa
      181 ggacggccaa ctttcatcct aaaaccacaa actatgtgca tgagataatc aagtttatca
      241 atgaactcat tgataaaggg ttcgcatatg aatccgcagg tgatgtctat tttgatgtaa
      301 gaaagttcga gcagtatggt atgctttcac ataggaatcc ggacgatatg agatcaggct
      361 caaggataga ggtttctgat ataaagactg acccgcttga ctttgccctc tggaaaaaag
      421 caaaagagaa tgaaccttca tgggaaagtc catggggaaa aggtagacct ggctggcata
      481 ttgaatgttc ggttatggct accgagcttt tgggagagtc atttgacata catgcaggag
      541 gaaatgatct tatattccca catcatgaga atgaacgcgc ccagtctatg gcacacagcg
      601 gaaatgattt tgccaattac tggatgcata acggtatgat aaaggttgcc ggagataaaa
      661 tgtcaaagtc tataggaaac atctggcttg tacgtgatgt tgtgagcaag tacggagctg
      721 atacggtcaa agtgtttgtc ctttccaagc actacagagt tccgcttgat ttcagcgagg
      781 aaggactgca ggcacaaaaa aaatccgtaa gcagagttca gcaggcttta aaagatgcgg
      841 aagatttttt tgccggtacg gtgccgtatg taaaaaacac tgagtatatg aaatcgaaga
      901 tagagacttt caaacaggca ttatcagacg acttcaacac ctcaaaggct gtcgccctta
      961 tatttgatct ttcggcagag cttaataaag cgttgtctga aaagaatgaa acccatgtaa
     1021 ttgagaacta tcaccttata cgtaatgagt tcgggccggt tctggggatc tttgaaatac
     1081 aggaaaccca gcagaaaagt tcgacagacg atacggataa agtaatgcag gtcttgatac
     1141 aactgagaaa tcaagccaga accgacaaaa attatacttt gagtgacagt atacgcgacg
     1201 gtcttgcatc aatcgggata cagctcaagg atactcctga aggaactaaa tacataatac
     1261 agtaaaaaac catgcttagg catggttttt tagtttttaa gcgggtatcc ggatacttca
     1321 aagaactgct ttttgaactg cagaaatcta tcttcttcta tagcttttct catatccttg
     1381 acaaggctta tattaaagtg gaggttatga atgcttaaaa gcatctttcc cagaatttca
     1441 tccctcttga aaagatggtg gatatagccc tgtgaatgat tacggcaggt atagcaggaa
     1501 cagttttcat ctataggctt ttctatataa gcacattttg aagctttaag gtttattctt
     1561 ccattgtatg tcaaagctgt gccatgccgt cccattctgg taggaagaac acagtcgaac
     1621 atatccatgc cgttttccac tgaaagaacc atcatggtgg gagaacctat tcccattata
     1681 tacctgggtt tatcggcggg cagctgcgta ccgaacgact gaagaatttt ttcggactcc
     1741 tggtactttt ctcctaccgc aagtccccca agagcatatc cgtcaaaagg aaccgaagtt
     1801 atctgctcaa ggctcctctg ccgaagatcc tcaaacaatg ccccctgtac aattccaaaa
     1861 agagcttgat cttttctgct atgactttca aaagatcttt tagcccagcg gaaggttctg
     1921 tcaatggaag aagatacata atcatgatcg gcatatgccg taatacactc gtcaaatgcc
     1981 atggctatgt cagaaccgat gctctgctgt atcttcatgg atatttcagg agtgattttt
     2041 aacttggaac cgtccagagg tgaacggaag ctaacgcctt gttcatctat aatacggtct
     2101 ttgagggaaa atacctggaa tcctccgctg tctgttagaa taggtttttt ccagttcata
     2161 aaactgtgaa gaccgccgaa agaatcaaga acatcaagac cgggtctgag aaaaagatgg
     2221 aaggcatttc caagaattat ttcagaaccg acatcttcaa ggctttgacc tgtaagagct
     2281 tttaccgttg cattggttcc tacgggcatg aagacaggag tttcaattat cccgtgtttg
     2341 gtatgtattc tgcctctgcg ggcattggta tatctgtcaa tgtgtaaaag ttcgtacttt
     2401 ataggtgaaa cgtcgtccat aaaatcctcc ggttttttaa attttaaggc cgtccctgag
     2461 taaatcaaaa cttataaaaa actcaggtat tcctatacag tacgggccta tttcatactg
     2521 ctgaaaaaac att
//
LOCUS       DAOSHR010000263         2526 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_40014_length_2526_cov_4.583974, whole genome shotgun sequence.
ACCESSION   DAOSHR010000263 DAOSHR010000000
VERSION     DAOSHR010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40014_length_2526_cov_4.583974"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /locus_tag="PLU28_10455"
     CDS             complement(<1..714)
                     /locus_tag="PLU28_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOG35521.1"
                     /translation="MKFPAEILIGIISVFIVLILLLSKKIVKPFLKPRNWRKYSESVT
                     AYLYLLPSFVVLGIFVFYPIVYSFVLSFFKWDFKNQQNPMFNGLENYQKLFTLRNGIE
                     ASFNTAFFNTVLVLMTFIFLMHVIFDLKKIKDKKYTKLMFVMTAVGLAFVIFGRFVPM
                     VVIIVVSIAMLCVYTVLAAMKFIEGSSDRLMGRLILFFVFYFACFKIGIPEIINYLSL
                     AKEQSDFIKSIWNTSHYVIL"
     gene            complement(731..2062)
                     /locus_tag="PLU28_10460"
     CDS             complement(731..2062)
                     /locus_tag="PLU28_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0046034 - ATP metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HOG35522.1"
                     /translation="MKPSNFIRFKSNYYGKLDKLPLIGLSGKVSRIVGMTIEAIGPDA
                     SIGDLCGIKLKDGNTALCEMVGFSDNKTLLMPFDEINGLYVGAPVKKLDKKVSLKADQ
                     SILGHVLDGIGRPMDSSGAIFKDEVPIYSPPPNPLIRKKIEQPLSVGVRAIDGLLTLG
                     KGQRIAIMAGSGVGKSTLLGMIARNTEADINVIGLIGERGREVREFIERDLGEEGLKK
                     SVVLVSTSDQPALVRVKALYTATAISEYFRDKGYNVLLMVDSLTRWAMAQREIGLSIG
                     EPPATRGYTPSVFGNLPKVLERAGNSDKGTMTAIYTVLVEADDMNDPIGDTVRGIVDG
                     HIVLSRKMADTGHYPAIELLGSVSRLMSSVVDSAHLQAGRVVKEIISTYREAKDLIEV
                     GAYKSGMNKKIDVAISEINSINSFLKQEVDEREDFKTIKEKLLQISKRCSV"
     gene            complement(2049..>2526)
                     /locus_tag="PLU28_10465"
     CDS             complement(2049..>2526)
                     /locus_tag="PLU28_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35523.1"
                     /translation="AIVEEGKKQADLFYRQNVEQKNKEFSVIIENVKKSLYEYTANYE
                     TLLEDSLIEAFKKLNLKFLKQEIVENPLWMKTIISELKNRLSSFNSFSMRMNERTFEK
                     AKEFLEESSIKSIKTDNSLSDNEIFVDTEYGVFDISAQSYIEDMIKIFEESKNETK"
BASE COUNT          798 a          544 c          344 g          840 t
ORIGIN      
        1 gagaataacg tagtgtgaag tattccatat cgattttata aaatcagatt gctccttggc
       61 aaggctaaga tagtttatta tttcaggaat tcctatttta aaacaagcaa aataaaacac
      121 aaagaaaagt ataagccttc ccataagtct gtcagaagaa ccttctataa acttcattgc
      181 tgccaaaaca gtataaacac aaagcattgc tatagatact actattatta caaccatagg
      241 aacaaatctg ccaaaaataa caaaagcaag accaactgct gtcataacaa acataagctt
      301 tgtatacttt ttatctttaa tcttttttaa atcaaatatc acatgcatta aaaatataaa
      361 cgtcatcaag acaaggactg tattaaaaaa agccgtgtta aagcttgctt ctataccatt
      421 tcttaatgta aaaagcttct gataattttc aagtccatta aacataggat tttgctgatt
      481 tttaaaatcc catttaaaaa agctaagaac aaaagagtat actataggat aaaaaacaaa
      541 aattcctaac accacaaaag aaggaagaag atagagatac gcagtcacac tctcactata
      601 cttccgccaa tttcttggtt ttagaaatgg tttaacaatc ttttttgaca aaagaagaat
      661 caacacgata aagacagata taattcctat aagaatttct gctggaaatt tcatcttacc
      721 cctcctgatt ctaaacagaa catctcttgg atatttgtaa taacttttct tttatggttt
      781 taaaatcttc tctttcatct acttcttgtt tcaaaaagct atttatgctg tttatttctg
      841 aaatagccac atctatcttc ttattcatac cagatttata cgcaccaact tctatgagat
      901 ccttggcttc acgatatgtc gatattatct ctttaacaac tctacctgcc tgaagatgag
      961 cactgtcaac caccgaactc ataagtctgc taacacttcc aagaagctct atcgcagggt
     1021 aatgccctgt atcggccatc ttcctggaaa gcactatatg cccatcgacg atacctctta
     1081 ctgtatctcc tatcggatca ttcatatcat cagcttcaac cagtaccgtg tatatagctg
     1141 tcatggttcc tttgtcagaa tttcccgctc tttccaaaac tttaggcaga ttaccaaaaa
     1201 cacttggagt atatcccctt gtagccggtg gctcgcctat tgaaagtccg atttctctct
     1261 gagccattgc ccatcgggtt agagaatcta ccataagaag aacattatat cctttatccc
     1321 taaaatattc agatatagct gtagctgtat aaagagcctt tactcgtact aaggcaggtt
     1381 ggtctgaagt ggagacaagt acaacagact tcttaagtcc ttcttctcct aagtctcttt
     1441 caataaactc tctgacttct cgtccgcgtt cgcctataag acctataacg ttaatatcag
     1501 cttcagtatt ccttgcaatc ataccaagca gagtactttt ccccactccg ctacctgcca
     1561 taattgcaat tcgctgccct ttgccaagag taagcaaacc atcaatcgca cgtaccccga
     1621 ctgaaagcgg ttgctctatt ttttttctta taagcggatt aggaggcggt gagtatatag
     1681 gtacctcatc tttaaatatg gctcctgaac tgtccatagg tcttcctatt ccgtcaagta
     1741 catgtcctag tatactctga tcggccttaa gtgaaacttt cttatctagc tttttcactg
     1801 gcgctccgac ataaagaccg tttatttcat caaacggcat taacaaggtt ttattatctg
     1861 aaaatcctac catctcgcat aaagcggtat taccatcctt cagttttata ccgcaaagat
     1921 cccctatgga agcatctggt cctatcgctt cgatagtcat tccaactatc cgagatactt
     1981 ttccgctaag acctatcaaa ggtaacttat caagttttcc ataataatta ctcttgaatc
     2041 tgataaaatt acttggtttc atttttgctt tcctcaaaaa tctttatcat atcttctata
     2101 tagctttgag cagatatatc aaaaacccca tactcagtat cgacaaaaat ctcattatca
     2161 gacagagaat tatcagtttt tatactttta atagaagatt cttcaagaaa ttcttttgct
     2221 ttttcaaatg ttctttcatt cattctcata ctgaagctat taaaactgct cagacgattt
     2281 ttaagttctg aaattatcgt tttcatccat aaaggatttt ccactatctc ctgcttaaga
     2341 aatttaagat tcaatttctt aaaagcttct ataagagagt cttctaaaag tgtctcatag
     2401 tttgctgtat actcataaag actctttttt acattttcta ttataacaga aaactcttta
     2461 tttttctgtt ctacattctg tctgtaaaaa agatcagctt gcttttttcc ttcctcaact
     2521 atagct
//
LOCUS       DAOSHR010000264         2524 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_40053_length_2524_cov_4.173350, whole genome shotgun sequence.
ACCESSION   DAOSHR010000264 DAOSHR010000000
VERSION     DAOSHR010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40053_length_2524_cov_4.173350"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="PLU28_10470"
     CDS             complement(<1..244)
                     /locus_tag="PLU28_10470"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOG35524.1"
                     /translation="MELQANPMSFSSGKNNQIGLKLDTTYNNGSAETEFLLDESYSGW
                     RGMVHGGILSTILDDTMAWAVISLEVTPVTVKMDLRY"
     gene            complement(280..963)
                     /locus_tag="PLU28_10475"
     CDS             complement(280..963)
                     /locus_tag="PLU28_10475"
                     /inference="COORDINATES: protein motif:HMM:NF025865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOG35525.1"
                     /translation="MAYSEIINLRHSTRSFDNFLLSQEQTGEIKKILESTESISGQRL
                     DWRVEKIEQACSYQLFAPVKEKNGISFIEYGFQGEQAVIGLTALDLGTCWKGYSNSFG
                     APCFIEFGKEKSSKKNLLIKTFTRNGVKKELKDLIKNGSAFLTNENSFLLNCCRMAPS
                     AINRQPWSFDLLSEKKIKITVQKAANADTYSIDLGIVLSHFILGCRDLGISIKKTILS
                     ENSCVFELN"
     gene            1296..1760
                     /locus_tag="PLU28_10480"
     CDS             1296..1760
                     /locus_tag="PLU28_10480"
                     /inference="COORDINATES: protein motif:HMM:NF015898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOG35526.1"
                     /translation="MPKYSWIENMIITYLYSHRQMTGYDFIKYCKENDVNISAGSVYP
                     ILKRLSEKNMVKVDLQGRKKIYTLINGENSNAAERSHIYLKSNINKIGTTISCSCDLI
                     PASLREEVSSYLKTLCSTEWKNPEAIKKLEEETRRLGENILKHLNFLEGGRS"
     gene            1757..>2524
                     /locus_tag="PLU28_10485"
     CDS             1757..>2524
                     /locus_tag="PLU28_10485"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOG35527.1"
                     /translation="MKIIVVGGVAGGATAAARLRRLDEKAQITVYERGEYISFANCGL
                     PYHIGDVIQDRERLLLETPETFKEKNNITVKVKSNIVKINREKKTVLVKDLLTSREYE
                     DSYDFLLLSPGAEPIKPSIAGIDSPNIFTLRNINDMDRIIKYSDEHKVKKVAVIGAGF
                     IGLEAAENFLHKGKNVTIIEMGPQVLSPIDFEMASLLHSHITDKGAELILRDGVEKFA
                     YNGELTRIHLKSSRTVDAQIVIFAIGVSPEVTLAKISG"
BASE COUNT          853 a          428 c          472 g          771 t
ORIGIN      
        1 tatacctgag atccatcttc actgtaacag gggttacttc aagagagatt acagcccagg
       61 ccatagtatc gtcaagaatg gtagaaagta ttcccccgtg aaccattccc ctccatccgc
      121 tgtagctttc atcgagtaaa aattctgtct cagcacttcc gttattgtaa gtagtgtcaa
      181 gctttaaacc aatttggttg ttctttccgc tggaaaagct catgggattt gcctgaagtt
      241 ccaattttat cagcactcct ttagcagtta taagaaatat taattaagtt caaaaacaca
      301 actgttttct gaaaggatag tttttttgat gcttattcca agatctctac agccaagaat
      361 gaaatgtgac agcactatac ctaagtcaat gctgtatgta tctgcatttg ctgctttctg
      421 gacagtaatt tttatttttt tctctgagag aagatcaaaa gaccacggct gtctgtttat
      481 ggctgacgga gccattctgc aacaattaag caaaaaagag ttctcattag ttagaaaagc
      541 cgaaccgttt tttataagat ctttaagttc cttctttact ccattacgag taaacgtttt
      601 tataagtaag ttctttttgc tgcttttttc ttttccgaac tctataaagc acggagcgcc
      661 aaaagagttt gaataacctt tccagcatgt gcctaaatcc aaggctgtaa gcccgatgac
      721 tgcctgttca ccctgaaaac catactctat aaagcttatg ccgttttttt cttttaccgg
      781 agcgaacagc tgataagaac atgcctgctc tattttttca acacgccaat caagtctctg
      841 acccgaaatg ctctctgtgc tttccaagat tttctttatt tcaccagtct gttcctgcga
      901 aagtaaaaag ttgtcaaaag atcttgtaga atgacgtaaa ttgataattt cagagtatgc
      961 catatcatgc tcctttcaat acgataattt tttctcatct ctaagaaatt atatcacttt
     1021 ttcctgaagt tttatgatac tcctttacag ataacgactg catcgctttt cagtttctct
     1081 tttaactgag tagtctgata attttaatag aaaatcactt gtacaattca aaaaaagaat
     1141 tattttatgt ttagatgggt ttattatatg ttatttgttg ttaactactg ctaatcatct
     1201 ttttttacaa ggaaaatata ctatataatt atatcgtaat tgatatatcg attacgatat
     1261 aaaataatat agaaaacaat cttaaggaga taagaatgcc aaagtactca tggatagaaa
     1321 atatgattat aacttatctt tatagtcata gacagatgac cggatacgac tttataaaat
     1381 actgtaaaga aaatgatgta aacatttctg ctggaagtgt atatcctatt ttaaaaaggc
     1441 tcagcgaaaa aaatatggta aaagttgatt tacagggcag aaaaaaaatt tatacactga
     1501 taaacggaga aaattctaat gcagcagaaa gatcacatat ttatcttaaa agcaatataa
     1561 acaagatagg aactactata agctgcagct gtgatcttat acctgcttca ttgagagaag
     1621 aggtaagttc atatcttaag actctttgtt caacagaatg gaaaaatcct gaagctataa
     1681 aaaaacttga agaggaaaca cgaagactcg gtgaaaatat tcttaaacat ctaaatttcc
     1741 tagaaggagg aagatcatga agataatagt agtgggagga gtagcaggtg gggcgactgc
     1801 ggcggcaagg ttgagacggc ttgatgaaaa agcacagata actgtttatg aaaggggaga
     1861 gtatatcagt tttgcaaact gtggactgcc ctatcatata ggagatgtta tacaggacag
     1921 ggaaagactt ttgcttgaaa cccctgaaac gtttaaagaa aaaaacaata ttactgttaa
     1981 agttaagtcc aacatagtaa aaattaatag agaaaaaaag acagttcttg taaaagatct
     2041 gcttacatca agggagtatg aggacagtta cgattttcta ttactgtcac ctggagcgga
     2101 gcctataaaa ccttcgatag ctggtataga ctctccgaac atatttactt taagaaatat
     2161 aaacgatatg gacagaataa taaaatactc cgatgaacat aaagtaaaga aagtggcggt
     2221 tataggggca gggtttatag ggcttgaggc tgctgaaaat tttttgcata aagggaaaaa
     2281 cgtaaccata attgaaatgg gaccgcaggt actttccccg atcgactttg agatggcatc
     2341 tctgctgcat tcacatataa ccgataaagg agcggaactt attctcaggg atggtgtaga
     2401 aaagtttgca tataacggtg agcttacaag gatacatctt aagagctcaa gaacagttga
     2461 tgcacagatt gtgatatttg caataggagt atcgccggaa gtaaccttgg caaaaatttc
     2521 cggt
//
LOCUS       DAOSHR010000265         2476 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_41239_length_2476_cov_4.137960, whole genome shotgun sequence.
ACCESSION   DAOSHR010000265 DAOSHR010000000
VERSION     DAOSHR010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41239_length_2476_cov_4.137960"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..946)
                     /locus_tag="PLU28_10490"
     CDS             complement(<1..946)
                     /locus_tag="PLU28_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35528.1"
                     /translation="MILLLISEEKVYRKFIIFIFFLIFIFFLISIGRQIYSYKHTKFE
                     YATFSFYSTDGENWNSLSFLEKEKEFSNTLLYLKTGVLNIDYEDLSLYIQGYLKNYIQ
                     LLKTPFQIYVDQKLAYSYHFPFQDYNHFEKPSFLFLLYPQSIKSELILKIDLGQVGLK
                     ISQIHNNFLLSSSEVLFFKAILDSFEYLIFGTFLAVFAFVFIPIIKKLMQFNKITYFE
                     LVILSFLVSSYLFAQSGVVGLLFGEKNAEYFMWWVSLSFIGLYLSTVFIKESNDSKDV
                     LVYVVRNIQIAFMVYEVILKGIIIVLSLNSKLEWGLFFI"
     gene            complement(948..1454)
                     /locus_tag="PLU28_10495"
     CDS             complement(948..1454)
                     /locus_tag="PLU28_10495"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOG35529.1"
                     /translation="MLKMIFIFFKIGFLAFGGGWSIVGLMQEEMIKNGYLSFAQFTEA
                     VSISQMTPGPVAVNMATYVGYRQYGIIGAVLNTVFLVLPPVIILFSATILGKFIKVDK
                     KKLFSSLGIATVILVCLTFLQMLLPNVTEYRIIIVAFITFLLYIKTKIDPIYIILGAG
                     FLGAFILG"
     gene            complement(1447..1986)
                     /locus_tag="PLU28_10500"
     CDS             complement(1447..1986)
                     /locus_tag="PLU28_10500"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOG35530.1"
                     /translation="MIFNLFWMFLKVSALTLGGGYAMVPVMDKQLQSRGIMIEKDFYE
                     ILTIAQSLPGPIAFNVALLCGKKVAGIKGAFASTLGVLIPPFFAIVLAASIIKKYSDN
                     VYVKGFLKGSYGAVIGMVGGVLYKMASKQQWNLFKIITCIAGVLILLANKDIVVVVFL
                     FVIILNYIYESKKGSRTNA"
     gene            complement(2002..>2476)
                     /locus_tag="PLU28_10505"
     CDS             complement(2002..>2476)
                     /locus_tag="PLU28_10505"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HOG35531.1"
                     /translation="QGGSTGGTDIIAMIINKYKDISPGRVLLMVDALVIGSSFLVFKS
                     IEKTIYGFVAIAVSTYVIDIVLQGTKQSAQIFVISEKYEKIADRITKEMHRGVTIIDG
                     KGWYSHESKNIIMVLVRKNETQNILRVVHEEDQRAFTSISSVTGVYGKGFESIKM"
BASE COUNT          884 a          481 c          346 g          765 t
ORIGIN      
        1 taataaaaaa caaaccccac tccagttttg agttaaggga aagcacaata ataattcctt
       61 taagaatcac ttcatatacc ataaatgcta tctgtatatt gcgaactaca tatacgagaa
      121 catccttgct atcatttgat tcctttataa aaactgtgct taagtataat ccaataaaag
      181 aaagactaac ccaccacata aagtattcag catttttttc tccaaaaagt aagcctacaa
      241 ctcctgattg tgcaaataga tacgaagaaa ccagaaaact cagtataacc aactcaaaat
      301 acgtaatttt gttaaattgc attaattttt tgatgattgg aataaataca aacgcaaaga
      361 ctgcaaggaa agtgccgaaa atgagatact cgaagctatc cagaatagct ttgaaaaaaa
      421 gaacttccga tgaggaaaga agaaaattgt tgtgaatctg cgaaattttt aatcctacct
      481 ggccaaggtc aattttgaga attaactcac tttttattga ttgtggatat agcagaaata
      541 aaaaagaagg tttttcaaag tggttgtaat cctgaaacgg aaaatgatat gaatatgcaa
      601 gcttttgatc aacatagatc tgaaaaggtg tttttaaaag ctgtatataa tttttcaaat
      661 atccctgaat ataaagagag aggtcttcat aatcaatgtt caaaacacct gttttaagat
      721 aaagcaaagt attggaaaac tctttttcct tttccaaaaa agaaagacta ttccaatttt
      781 caccatcagt actgtaaaaa gaaaaagtag catactcaaa ctttgtatgc ttatagctgt
      841 aaatctgtcg cccaatcgat attaaaaaaa atataaagat taaaaaaaat ataaagataa
      901 tgaattttct atatactttt tcttcggaaa taagcagcaa tatcacctta ccccaatatg
      961 aaagctccta gaaatccggc gcctagaatt atgtatatag gatcaatctt ggtttttata
     1021 taaagcagaa atgtaataaa tgcaacaatg attattctgt actccgtcac gttcggaagc
     1081 aacatctgca aaaaagtaag acatacaaga ataacagttg caattccaag agatgaaaaa
     1141 agcttttttt tatccacttt aataaattta ccaagaatag tagcagaaaa taatataatt
     1201 accggaggaa gaacaagaaa aactgtattt aggacggcac ctattatgcc atactgtcta
     1261 tatcccacat aagttgccat atttacagct actggtcctg gggtcatctg cgaaatagaa
     1321 actgcttccg taaactgtgc aaaagatagg tagccgtttt taatcatttc ttcctgcata
     1381 agtccgacta tggaccatcc acctccaaaa gccagaaatc ctattttaaa gaaaataaag
     1441 atcattttaa gcattagttc tacttccttt tttgctctca tatatataat ttaagattat
     1501 aacaaataaa aaaactacta ctactatatc tttatttgca agcaatatca aaacacccgc
     1561 tatgcaagta ataatcttaa aaagattcca ttgctgttta gaagccattt tgtacaagac
     1621 ccctccaacc attcctatca cagcaccata gcttcctttc aaaaaacctt ttacataaac
     1681 attatcagaa tattttttta ttatacttgc ggcaagtact atcgcaaaaa atggaggtat
     1741 aagaactcca agagtacttg caaatgctcc ttttattcca gcaacttttt taccgcagag
     1801 taacgcaaca ttaaaagcta taggtcctgg aagagactgg gcaatagtaa gaatttcata
     1861 aaaatctttc tcaatcataa ttcctctgct ttgcaactgt ttatccataa ccggaaccat
     1921 ggcatatccc ccgcctaaag taagtgccga tactttcaaa aacatccaga acagattaaa
     1981 aatcatactt tctccttttc attacatctt tatgctttca aatccctttc cgtatacacc
     2041 ggtaacagag cttattgaag taaaggcgcg ctgatcctct tcatgaacta ctcttaggat
     2101 attctgagtt tcatttttcc tgaccagtac cattattata ttcttgcttt catggctgta
     2161 ccagcctttt ccatcgatta tagttactcc tctgtgcatc tctttggtta tacggtctgc
     2221 aatcttctca tatttttcag atattacgaa tatttgagcc gactgcttgg taccctgtaa
     2281 cactatatct atcacataag tacttacagc tattgcaaca aaaccatata tcgtcttttc
     2341 aatagattta aaaaccagaa acgaagagcc tattacaagt gcatccacca tcagtagtac
     2401 tctgccggga cttatatctt tatacttgtt tattatcatg gcaataatat ctgttccgcc
     2461 cgtacttcct ccctga
//
LOCUS       DAOSHR010000266         2466 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_41468_length_2466_cov_3.605973, whole genome shotgun sequence.
ACCESSION   DAOSHR010000266 DAOSHR010000000
VERSION     DAOSHR010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41468_length_2466_cov_3.605973"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            237..932
                     /locus_tag="PLU28_10510"
     CDS             237..932
                     /locus_tag="PLU28_10510"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOG35532.1"
                     /translation="MPELKSVYVVDDEEKILELIKSYLLKDGYMVKTFKDGFSALKEF
                     ETNQPDMFIIDIMMPGMDGYSLCTEIRKKSKVPIIMVSAKDTDIDKVVGLEIGSDDYI
                     SKPFSPRELMARVKSLFRRIDDKYYIESTDTAVKYKDLVIKPNERSIYKDNNNLEFTA
                     KEYDFMLYLLNAKNKLVSKAELLKAVWGFSENEDTRVIDDVLKRIRKKLESCGSKVQI
                     VTIWGYGYRISQE"
     gene            943..2376
                     /locus_tag="PLU28_10515"
     CDS             943..2376
                     /locus_tag="PLU28_10515"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOG35533.1"
                     /translation="MKTTITRRITLPFLLIILFSFISVIVMFRVGLERYSMHQIKKNL
                     LNATQNIRMIMRDDILNKKQFKTFSGEVEADDFILNEKAMREIRQIVQKSSFSFRSQT
                     IIVDKQTGLLFPKSGSNEDTLKVIEKIIPELDGESQEVQTKTIRIDDKEYTIAFRPFL
                     NNNELSAKPRVWIVVYTDNTVFDELISSTMYIMIIIIVIATACALAAGVIIARSIASP
                     IIKLKERAAKLARRDFETKLTINTNDEIQELAVCMNEVADNLRNYDISQKNFIQNASH
                     ELKTPLMSIQGYAEGIKDEVFQDKEKALDVIIEESQRLRTLVEEIIYLSKIESSGQPI
                     RLEKKRLNEVIDRSVEKISGLMVGNTIKIEKKYLSDRWLLMDEDKMTRAFINILGNCL
                     RFSESIITIEVSDSHGSCSIKIKDDGPGFDMQEEDKIFERFYKGKKGNTGLGLAITKA
                     IIEKHSGSIRAYNCELKGACFEIILPL"
BASE COUNT          936 a          378 c          491 g          661 t
ORIGIN      
        1 tgccgcatca tctgcaaaca ctacagaaga aaaaacaagt acaagagcca acaccataaa
       61 aatacttact gtttttttca tactttcacc atcctcctta attatattta cgcttttatt
      121 atatataaga actgtagcaa gaaaatatga gaatctggca aaattgtgag aaatggaaaa
      181 gaacttttga gtatgctaca atatatttaa gttgaatgat tttaaaggag gtcagtatgc
      241 ctgagctaaa gagtgtctat gtagtggacg acgaagaaaa aatattagaa ctcataaaat
      301 cttatctttt aaaagatgga tacatggtca aaacttttaa agatggtttt tcggctttaa
      361 aagagtttga aaccaatcag cctgatatgt ttataataga tatcatgatg cccggcatgg
      421 acggatactc actttgtact gagataagaa aaaaaagtaa ggttcccata ataatggttt
      481 ctgccaagga tacggacata gataaggtgg taggtcttga gatcggaagc gatgattata
      541 tatcaaagcc tttcagtccc agagaactta tggcaagagt gaagtcactt ttcagacgta
      601 tagacgataa gtattatatt gaaagtaccg ataccgctgt caagtacaag gatcttgtca
      661 taaaaccaaa cgaaagaagt atctacaagg acaataataa tcttgagttt acagcaaaag
      721 agtatgattt tatgctctac ctattgaatg ccaaaaataa gcttgtttca aaggctgaac
      781 tgctcaaagc agtatgggga ttttctgaaa atgaagatac aagagttatt gacgatgttc
      841 tgaaaaggat acggaaaaag cttgaaagct gtggctcaaa agtacagata gtaaccatat
      901 ggggatatgg ttaccgtatt tctcaggagt aggtgaatac agttgaagac caccataacc
      961 agaagaataa cacttccttt tcttttgata atactttttt catttatatc agtaattgtt
     1021 atgttcagag ttggacttga gaggtacagc atgcaccaga taaagaaaaa tcttcttaat
     1081 gcaacacaaa acatccgtat gataatgcgt gatgatattt tgaataaaaa acagtttaaa
     1141 acattttccg gagaagtaga ggcagatgat tttattttaa atgaaaaagc aatgagagag
     1201 atacgtcaga tagtacagaa aagttctttt tccttcagaa gccagacgat catagtagat
     1261 aaacagaccg gtctgctttt tccaaaatca gggtcaaatg aagatacctt aaaagtaata
     1321 gaaaaaatca ttccggagct tgatggagaa agtcaggaag ttcagacaaa aacgatcaga
     1381 attgatgaca aagagtatac catagccttc agaccatttt tgaacaacaa tgaactttct
     1441 gccaaaccaa gagtatggat agtggtgtat acagataata ctgtttttga tgagcttata
     1501 agctcaacca tgtatataat gataataatt atagttatag ctacagcctg tgcccttgca
     1561 gccggagtca taatagcaag atctatagcc agccctataa taaagcttaa ggaaagggca
     1621 gccaagcttg ccaggagaga ctttgaaact aaacttacca taaatacaaa cgatgaaata
     1681 caggaactgg cagtatgtat gaatgaagtg gcagacaatc tgagaaatta cgatatttcg
     1741 cagaaaaatt ttatacagaa tgcctcgcat gaactaaaaa cacctcttat gtcgatacag
     1801 gggtatgctg agggaataaa agatgaagta tttcaagaca aagaaaaagc tctggacgta
     1861 ataatagaag agagtcagcg tcttagaacc ctagtcgaag agataatata cctttcaaag
     1921 attgaaagct ccggtcaacc catccgtctt gaaaaaaaga gactgaatga agtaatagac
     1981 agaagtgtgg aaaaaatttc aggtcttatg gtaggaaata caataaaaat agaaaaaaaa
     2041 tatctttcgg acagatggct cctcatggat gaagataaga tgaccagagc ttttataaac
     2101 atactcggaa actgtctgag attctccgaa agtattataa caatagaagt atcggacagt
     2161 catggtagct gcagcataaa aattaaagat gacgggccgg gttttgatat gcaggaagaa
     2221 gataaaatct tcgagagatt ctacaagggc aagaaaggaa ataccggtct gggacttgcc
     2281 ataaccaaag caataataga aaaacattcc ggcagtataa gagcttacaa ctgtgagtta
     2341 aaaggagctt gctttgaaat aatccttccg ctatagagat aaaaaaaaag acattcatat
     2401 aatgaatgtc ttttttttta ccagagtatt tggaaaactt gggaaaaagt gttattctag
     2461 taatag
//
LOCUS       DAOSHR010000267         2451 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_41832_length_2451_cov_4.569282, whole genome shotgun sequence.
ACCESSION   DAOSHR010000267 DAOSHR010000000
VERSION     DAOSHR010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41832_length_2451_cov_4.569282"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="PLU28_10520"
     CDS             complement(<1..473)
                     /locus_tag="PLU28_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35534.1"
                     /translation="MENIKKIPKDILIISALFLLLPFIVIPQWVYEYSTQKHFIFALF
                     FTVMAVFYLFSKKKENTLSLGWPHKFLFLFGISVLVSLISSFITNRQYFTWSLENAAY
                     TVLVISVCFIITNRFGKDMSFIETGLLFFMIGGTIIAFDGLLNKFTGYDLFFGKYG"
     gene            709..1422
                     /locus_tag="PLU28_10525"
     CDS             709..1422
                     /locus_tag="PLU28_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG35535.1"
                     /translation="MKRVLVIMFVLFLSVWAAAGNFSEYYSRFIAANSMQDAQAMKEV
                     VALLQADTSNASLTALYINAVTEYANWAVKDEKEREKLYQQAVDYGKKIIKDNKDNWY
                     LSYTLGAAYGRLAQFKGIVSSLFMLGDFDANIKKSIELNPSNYLGYVALGMRYRDVPW
                     PMNDMKKSEENFKKAIEKEPLYVNAYLELGMLYLKTNKKELAREMFNKVISMQPHPEW
                     KKQGEEAKKLAEDELKKIK"
     gene            complement(1443..2252)
                     /locus_tag="PLU28_10530"
     CDS             complement(1443..2252)
                     /locus_tag="PLU28_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35536.1"
                     /translation="MKYKFYIFIFLVILVFLFYPITIITESSGRIKVYCTGMVRYFYF
                     SDTQSSFSQGAGFFYKEFSYNNYAVLNAENSILRIEICHNAIQLQKEKETAYVFVSKD
                     YLPFISEDTSFASNKKWLTDEAKDINDYFNGKVIIPSKMFVIYANIPRSFHISPYVIF
                     VNSMKDMIHEFNHYTFGYYIKDGASDTWAEILCEINTLLYLKKKYPMLYESELELKSI
                     GFYPQQYGSKVIEFYKHFNYDEAKIFNFESKLIDSRKRLNDTVFYSLFVSE"
     gene            complement(2242..>2451)
                     /locus_tag="PLU28_10535"
     CDS             complement(2242..>2451)
                     /locus_tag="PLU28_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35537.1"
                     /translation="SKGLEASYQKGFINIVCDCLPEINGDTQNKTVYQSLCYSVHPSC
                     GGSLIYYTGNTLKGGNSEGYIILEV"
BASE COUNT          883 a          369 c          453 g          746 t
ORIGIN      
        1 ccgtacttac cgaaaaaaag atcgtagcca gtaaatttgt ttagaagtcc gtcaaaagct
       61 attatagtac cgcctatcat aaaaaatagc aatcctgttt caatgaagct catgtctttg
      121 ccgaacctgt tggttattat gaagcataca gatattacaa gaactgtata tgcagcgttt
      181 tcaagagacc aggtaaagta ctgtctgttt gttatgaaag aagatatgag agaaactaga
      241 acagatattc cgaaaaggaa caggaattta tgtggccatc ccagactcag ggtgttttcc
      301 tttttctttg aaaagagata aaaaacagcc attaccgtaa agaataaagc aaaaataaaa
      361 tgtttctgag ttgagtattc gtagacccac tgcggaatga caataaaagg taacaacaaa
      421 aataatgcag agataatgag aatatctttt ggaatctttt taatattttc cacaaaattc
      481 cctccttgaa taactatcaa atatatatta tcatattttt atgaaaaatc caataaagct
      541 aagagataaa aagagctaaa agtgttgtta aaagtggagt gcgatatcaa aaaaaaaaaa
      601 tattctttat tatctacaga gtaggtattg aataaagata aaattgaaga ttcaaggtat
      661 aattataata tgtgtaaaaa aatctaggga gggacaggag gttatactat gaaaagagtg
      721 ttagttatta tgtttgttct ttttttatca gtatgggcag cagccggaaa tttttccgag
      781 tactattcaa gatttattgc tgcaaacagt atgcaggatg ctcaggctat gaaagaggtt
      841 gtagctttat tgcaggcaga tacttccaat gcatctctta cggcacttta tataaatgct
      901 gttactgaat atgccaactg ggcggtcaag gatgaaaaag aaagagaaaa actttatcag
      961 caggctgtgg actatggtaa aaaaataata aaggataata aagacaattg gtatctttca
     1021 tatacgcttg gcgcagcata tggccgactg gcccagttca aagggattgt gtccagtctt
     1081 ttcatgcttg gagactttga tgccaacatc aaaaagtcga tagagcttaa tccttcaaat
     1141 tatcttggat atgtggctct tggaatgaga tacagggatg taccctggcc aatgaatgac
     1201 atgaaaaaat ctgaagaaaa ttttaaaaag gccattgaaa aagaaccttt gtatgtaaat
     1261 gcatatcttg agcttggaat gctttattta aagacaaata agaaagagct tgcccgtgag
     1321 atgtttaata aagtgatttc gatgcaacct catccggaat ggaagaaaca gggagaagaa
     1381 gcaaaaaagc tagcagaaga tgaactcaaa aagataaaat aatcaactgt tttaagaaaa
     1441 agctattctg aaacaaacaa actataaaat actgtatcat tgagcctttt tcgggagtca
     1501 atgagtttac tttcaaagtt aaatattttg gcttcatcat aattaaaatg tttataaaac
     1561 tctattacct ttgaaccgta ctgttgagga taaaatccta tgctttttag ttcaagctca
     1621 ctttcataaa gcataggata ttttttttta agataaagca gggtattaat ttcgcaaagt
     1681 atttctgccc aagtatcaga agcaccgtct tttatgtagt atccaaaagt atagtgatta
     1741 aactcatgaa tcatgtcttt cattgaattg acaaatatta cgtaggggct tatatgaaaa
     1801 gacctgggaa tatttgcgta aatgacaaac attttactcg gaataatgac ttttccgttg
     1861 aaataatcat taatgtcttt tgcttcgtcg gtaagccatt ttttattact tgcaaaggaa
     1921 gtgtcttcgg atatgaaagg cagatagtcc tttgaaacaa aaacatatgc ggtttctttt
     1981 tctttttgca gctgaatggc attatggcaa atttctatac gcagaataga attttcagca
     2041 tttaaaacgg catagttatt gtatgaaaac tctttgtaga aaaagcctgc gccttgagaa
     2101 aaagagctct gagtatccga aaaataaaaa taccgaacca ttccggtgca ataaaccttt
     2161 attctcccgg aggactcggt tataatagtt atggggtaga aaagaaaaac aagtataact
     2221 aaaaaaataa aaatgtaaaa tttatacttc aagtattata taaccttcgg aatttccacc
     2281 ttttaaagta tttccagtgt agtatatcaa acttccgccg catgaaggat gaaccgagta
     2341 gcataaactt tgataaactg ttttattttg agtatcgccg ttaatttctg gcaaacagtc
     2401 acatactatg tttataaagc ctttttggta agaagcttcc agtcccttgg a
//
LOCUS       DAOSHR010000268         2445 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_41979_length_2445_cov_5.203347, whole genome shotgun sequence.
ACCESSION   DAOSHR010000268 DAOSHR010000000
VERSION     DAOSHR010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41979_length_2445_cov_5.203347"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="PLU28_10540"
     CDS             complement(<1..317)
                     /locus_tag="PLU28_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOG35538.1"
                     /translation="MDIKIFDTLKGELVNFVPIKNDSVSMYVCGPTVYNLIHIGNSRP
                     MIVFDAFRRFLEYVGYKVTMVQNFTDIDDKIIIKAVKEGVPFNEISERYIVEYMKDAL
                     ALR"
     gene            complement(318..1775)
                     /gene="gltX"
                     /locus_tag="PLU28_10545"
     CDS             complement(318..1775)
                     /gene="gltX"
                     /locus_tag="PLU28_10545"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOG35539.1"
                     /translation="MKEVRVRFAPSPTGFLHVGGARTALFNYLFAKHHNGKMILRIED
                     TDAERSTKESEEQLISALTWLGIDWDEGPTVGGPYGPYRQSERTDIYRHKAAELMQAG
                     VAYEGYAYPQELENIHDALLENGEPPHYTQEMISKFDTPQRRKEFAEKGMSPVIYFKM
                     PRKDYILKDMIRGEVVFKEGSVGDFVLIRSNGLPTYNYAVVVDDILMEITHVIRGDDH
                     ISNTLRQLAIYEAFDAQTPYFGHVSMILGPDGKRLSKRHGATSVEEFKDRGYLPEALI
                     NYLVLLGWSHPQAKEIIPLTEMIEHFGMERVNPSSAIFDEVKLRWMNGMYIRQAQTER
                     IFKLSVPFIMESKVLTMDQIQGSKKWIMQAIELIKTSVEEISQIPSELSIFINEFEFD
                     LQNEEFKGYFEAEGVKDGLKHLYELINNDMLWTVESITDNIKSCIKIKKPDKKSFYMA
                     LRKILTNQFHGPDLVKTIHLIGRVRVQQRIERVVF"
     gene            complement(1778..2317)
                     /locus_tag="PLU28_10550"
     CDS             complement(1778..2317)
                     /locus_tag="PLU28_10550"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOG35540.1"
                     /translation="MDFTEKILSEKQIYKGKLLDVRKYEVVLPNGLKSGREVIKHIGA
                     SAVLAVDDENNIFMVRQYRFAAGEHLLEVPAGKFDRHGEDPLECARRELEEETGKKAE
                     FWSYLGYILTTPGFTDEKIHLFLAKGLFDGKTDPDSDEFIQLVRTPLAEFEKMIREGN
                     INDAKTIAVYTRAKLSGGI"
BASE COUNT          653 a          574 c          419 g          799 t
ORIGIN      
        1 attctcagag ccagggcatc tttcatatat tcaacaatgt atctttctga tatctcgtta
       61 aagggaacac cctctttaac agcttttatt attatcttat cgtcaatatc ggtaaagttc
      121 tgaaccattg taactttata cccgacatac tcaagaaacc ttctgaaagc atcaaaaaca
      181 atcatcggtc ttgaatttcc aatatggata agattataaa cggtaggtcc gcagacatac
      241 attgaaacgc tgtcattttt tatggggaca aagtttacaa gctctccttt aagagtatca
      301 aatattttta tatccattta gaacaccact ctttctatcc gctgctgaac cctgaccctt
      361 cctattaaat gaatggtctt tacaaggtcc ggcccgtgga actggttggt aagtatcttt
      421 cttagcgcca tataaaaaga ttttttatct ggtttcttta tctttatgca ggattttatg
      481 ttgtccgtta tcgactcaac agtccagagc atatcgttgt ttataagctc atagagatgt
      541 tttaatccgt cttttactcc ctcggcttca aagtatccct taaactcctc gttctgaagg
      601 tcaaattcaa actcgttaat aaatatgctc agttcggaag gaatctgcga aatttcttct
      661 accgaagttt ttataagttc aatagcctgc attatccatt ttttactacc ctgtatctga
      721 tccatggtaa gcaccttgga ctccattata aagggaacag aaagtttaaa tattctttcg
      781 gtctgtgcct gacgtatata cattccgttc atccatctga gcttaacctc atcaaatatg
      841 gccgatgacg gattcaccct ttccataccg aaatgttcta tcatttctgt cagcgggatt
      901 atctcctttg cctggggatg tgaccatcca agcagaacaa gatagtttat aagagcctca
      961 ggaagatatc ctctgtcttt aaactcctcg acagaagttg caccgtgtct tttggaaagt
     1021 ctttttccgt caggaccaag gatcatggac acatgcccaa aataaggcgt ctgggcatca
     1081 aaagcctcat aaatggcaag ctgcctcaga gtattcgaaa tatggtcgtc tcctcttatt
     1141 acatgagtta tttccataag aatatcgtca actacaactg catagttgta agttggaagg
     1201 ccgttgcttc ttataagtac aaaatctcct actgaacctt ctttaaagac cacctctcca
     1261 cgtatcatat ctttaagtat ataatccttg cggggcatct taaagtagat tacagggctc
     1321 attccctttt ctgcaaactc tttccttctc tggggagtat caaacttgct tatcatctct
     1381 tgagtatagt gaggaggttc tccattttca agcaaagcat catgtatatt ctcaagctct
     1441 tgcggatagg catatccttc ataggccaca ccggcttgca tcaattctgc tgccttatgc
     1501 ctgtaaatat cagttctttc actctgtctg taaggtccat aaggtcctcc cacagtcggt
     1561 ccttcgtccc agtcaattcc aagccatgtc agagcgctta taagctgttc ctcagactct
     1621 tttgtggatc tttcagcatc ggtatcctct attctgagta tcatcttgcc gttatgatgc
     1681 ttggcaaaaa gatagttaaa caatgctgtc cttgcaccgc ccacatgaag aaaccctgtt
     1741 gggcttgggg caaatcgtac tctaacctct ttcataatca tatacctccg ctcagtttag
     1801 ctcttgtata tacggcaatt gttttcgcat cattgatgtt cccttcacga atcatttttt
     1861 caaactccgc caaaggtgtt cttacaagct gaatgaactc atcagaatcc ggatctgtct
     1921 taccgtcaaa tagtcccttg gcgagaaaaa gatgtatctt ctcatctgta aagcctggcg
     1981 ttgtaagaat gtacccaaga taactccaga actctgcctt tttaccggtt tcttcttcaa
     2041 gctcccgtct ggcgcattcc agaggatctt caccatgtct gtcaaacttt ccggcaggaa
     2101 cctccagaag atgttcacct gctgcaaacc tgtattgacg aaccataaaa atattatttt
     2161 catcgtcaac cgccagcacc gcacttgccc ctatatgctt tatgacttcc ctgcctgact
     2221 ttaagccgtt tggaagaaca acctcatact tacgcacatc aaggagcttg cccttgtaaa
     2281 tctgtttttc agaaagaatt ttttctgtga aatccattta atttactccc tcttaagcac
     2341 taaaagagtt atatcgtctt caggctgttt gtccccaaga aatgatttaa gttcatggga
     2401 gattgcgttt actatgttta taggtgtttt gttttctaca gaacc
//
LOCUS       DAOSHR010000269         2445 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_41982_length_2445_cov_4.057741, whole genome shotgun sequence.
ACCESSION   DAOSHR010000269 DAOSHR010000000
VERSION     DAOSHR010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41982_length_2445_cov_4.057741"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="PLU28_10555"
     CDS             <1..691
                     /locus_tag="PLU28_10555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35541.1"
                     /translation="YTPLVVLILLLVLESARVIPSLGMPLIFMVSSLFSLIGAKKYNY
                     FKMSVKAISSGLSIMSILIGVGMFIQVMTMTGVRGFIVSSLINLPNYMLYFTVGLGVP
                     LFGAVSCYGSASVLGVPFLLAFIGGNDIIIAACISMLAALGDMMPPNSLAANFSAGLI
                     GEKKYFGIVKQSVVPILVTIVYSVSIIVFSRSLKGVMNNTLVFSVTAGITLLIIYLLD
                     VLRYRRGDKNA"
     gene            684..833
                     /locus_tag="PLU28_10560"
     CDS             684..833
                     /locus_tag="PLU28_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35542.1"
                     /translation="MLRIVYLVILVYFLIVLIVSMKKSREKRYEWIMGVMIAVPWILK
                     VLGVR"
     gene            835..1959
                     /locus_tag="PLU28_10565"
     CDS             835..1959
                     /locus_tag="PLU28_10565"
                     /inference="COORDINATES: protein motif:HMM:NF016817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HOG35543.1"
                     /translation="MKKINYRFWMFVICSFLMSIYTFMYYKGQTESEKIFSGSGVEQK
                     VMLSSYFEGIKNTVMDTEVFILQGDEPGGTAVILGGTHPNEPAGYITAVMLVESCSVL
                     KGKVIVIPRANMAAFSWTDPLEGSPDKFYVNMPQGGVRVFRFGSRATNTSIQWPDPVL
                     YYKGGNQVLNGAESRNLNRAYPGNPDGNPTEKLAYAIMELIRKENADLAIDLHEASPE
                     YTTVNALVAHQKAIDIAAEAVMYMEMDGLNISLEQSPKNLSGLSHREWGDNSQTMPFL
                     LEASNPSQGRLRGRTDSDLIVYGKDKYYFKAEKAGRLNAPYDENGAPLDLRVARHLTA
                     VNYIVQAYSSNFSEREVIFNFLPSYEDIMQKGIGSYLLQQ"
     gene            2099..2428
                     /locus_tag="PLU28_10570"
     CDS             2099..2428
                     /locus_tag="PLU28_10570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35544.1"
                     /translation="MSKMLNTTSFEGLNNKLRQHLNDEIMCIGKFGKDKAIIDESFDI
                     RDMLKKLSKIENFPQTILLERKYGYMIIKNDFTDEEYLKYKLNFKPSKKLLKKLGLLD
                     KKEDALE"
BASE COUNT          783 a          382 c          513 g          767 t
ORIGIN      
        1 atatactccg cttgttgttc ttatactttt attggttctt gagagtgcaa gggtaattcc
       61 ttcattgggt atgcctctta ttttcatggt atcatcgctt ttttcactta tcggagccaa
      121 aaagtacaac tatttcaaaa tgagtgtaaa agctatttca agcggtcttt ccataatgtc
      181 catacttata ggtgtaggaa tgtttataca ggtgatgact atgaccggag ttcgtggttt
      241 tatagtaagc tcgctgataa atcttcctaa ctacatgctt tattttacgg ttggtttagg
      301 tgttccgctt tttggtgcag tatcatgtta tggttcggct tctgtacttg gagtaccttt
      361 tcttcttgcg tttataggag gcaacgatat aataattgca gcatgtatat cgatgctggc
      421 ggcattggga gatatgatgc ccccaaactc cctggcagca aatttttcag ccgggctcat
      481 cggtgaaaag aagtacttcg gtatagttaa acagtctgtt gtacctattt tagtcacaat
      541 agtgtactct gtttcaataa tagttttttc cagaagtttg aaaggagtta tgaacaatac
      601 cctggttttt agcgttactg caggtataac tcttttgatt atatatctgt tggatgttct
      661 caggtacagg cgaggcgata aaaatgctta gaatagtata tctggtaatt ctggtctact
      721 ttttaatagt tcttatagtt tcaatgaaaa aatcaagaga aaaaagatac gaatggatta
      781 tgggtgtaat gatagctgtt ccctggatct tgaaagttct gggcgtgaga taaaatgaaa
      841 aaaattaatt atcgattttg gatgtttgtt atatgcagtt ttttgatgag tatctatact
      901 tttatgtatt ataaaggtca aacagaaagc gaaaagattt tctccggtag cggagttgaa
      961 caaaaagtta tgctcagctc ctattttgaa ggtataaaaa acacggttat ggacacagag
     1021 gtatttatac tgcagggaga tgaacctgga ggaacagcag tgattctggg aggcactcat
     1081 cccaacgagc ctgctggata tataactgca gttatgcttg ttgaaagctg ttctgttctg
     1141 aagggaaaag taatagttat tccaagggct aatatggctg cttttagttg gacagatcct
     1201 ctggaaggca gtcctgataa gttttatgta aatatgcccc aaggtggggt aagggttttc
     1261 agatttggat ccagagcaac aaacacttca attcaatggc ctgatccggt tctgtattac
     1321 aaaggcggaa atcaggtact taatggtgcg gaatctagaa atctcaaccg ggcctatccg
     1381 ggaaatccag acggaaatcc cacagaaaaa cttgcatatg cgataatgga acttatacgc
     1441 aaagaaaatg cagatcttgc aatagacctg catgaagcat ctccagaata tactacggta
     1501 aatgctctgg tcgcacatca aaaggctata gatatagccg cagaagcagt tatgtatatg
     1561 gagatggatg gattaaacat atctttggaa cagtcaccaa aaaatctttc aggtctttct
     1621 cacagggagt ggggagataa cagtcagacc atgccttttc ttcttgaggc ttcaaatcca
     1681 tcgcagggaa ggctgagagg cagaacagac agtgatctga tagtttacgg taaagataag
     1741 tactatttca aagcggaaaa agcaggtcgg cttaacgctc cttatgatga aaacggtgca
     1801 ccattggatc tgagggtggc aagacatctt acagcagtaa actatatagt tcaggcttac
     1861 agttcaaatt tttccgaaag agaagttatt tttaactttt taccttcata tgaagatata
     1921 atgcaaaaag gaataggaag ttatctttta cagcaataaa tgccgtttta cggcatttat
     1981 tttttaaatt attaactaaa ttctaaagta ttcgtgaagt atgatgtaag taattgaatt
     2041 atttgtgtta taatataata taactgttat aaaaattatt gaaaggatgg tccggcttat
     2101 gtccaagatg ttaaatacta cctcttttga aggattgaac aacaaattaa gacaacatct
     2161 aaacgatgag atcatgtgca tcggcaagtt tggaaaagac aaagccataa ttgacgaaag
     2221 ctttgatata agagatatgc tgaagaagct ttcaaaaata gaaaactttc cgcaaactat
     2281 acttctggaa agaaagtatg gatatatgat aatcaaaaat gactttacag atgaagagta
     2341 tcttaagtac aagcttaact ttaaaccttc aaagaaattg ctaaaaaaat taggattact
     2401 tgataaaaaa gaagatgctt tggaataaag catcttcttt ttttt
//
LOCUS       DAOSHR010000270         2442 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_42046_length_2442_cov_4.777964, whole genome shotgun sequence.
ACCESSION   DAOSHR010000270 DAOSHR010000000
VERSION     DAOSHR010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42046_length_2442_cov_4.777964"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1232
                     /locus_tag="PLU28_10575"
     CDS             <1..1232
                     /locus_tag="PLU28_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35545.1"
                     /translation="LLFVFIFLVALCLTGFSADQRKTLLVYTKGYAPSTGLVETQVNQ
                     EIVSLGKYRLVTRDDVVFREISMQLSGILEGGNAKEIQQLKADAVIFIQVNDENGYQK
                     FSKDGKTSWVDYEINTNYKLIDVASGQVMTVKTFVGTGSSNITPNVPYSQAMKNAKYN
                     AVYNVANEIVMELNTLFKIKGSIVNITSDGRVIINVGKDIGVYEGMVFQFAKDVTINE
                     DKFDTVQGKLLATDVKSSTSILKILELPKDFAVSDQGVYITETPNMSALRSRLALNGG
                     MYGIKYPVFSFDVIVDNYQGLAFGMNIGACLANINSKNVILLDTGFMLGYMIDLDAME
                     ATIPVQFGMLYDLSTDDIDKAAFSLYIKPGLKIAVNLGDFMGAYAQGGYIFEIPMNSI
                     KTNVNGITLQAGVEFKF"
     gene            1327..1980
                     /locus_tag="PLU28_10580"
     CDS             1327..1980
                     /locus_tag="PLU28_10580"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOG35546.1"
                     /translation="MIKGVIFDLFGTLIKVESLFLGIAGEIATQTGNEVHEIEKKIHT
                     LYNKHFLNYHLKEFQPERHYYQKVFKELKNEYKLAYSADWYVDYAYDTFSELRQYNDV
                     NFLNDLKQSNFKIAILTNADSFFVRNCIEKNAIAFDKLIISEEVRAYKPSGQIFQIAL
                     DEVKLKKEEVVFVGDNINVDVLGGNNFGIKTILIDRDDSKECYPKVTNLYQIAEFIR"
     gene            1985..>2442
                     /locus_tag="PLU28_10585"
     CDS             1985..>2442
                     /locus_tag="PLU28_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOG35547.1"
                     /translation="MYKLKSNYMPQGDQPQAIDDLMNGLNRNDRFQTLLGVTGSGKTY
                     TMANVIEKSGRPALIMSPNKILAVQLYREFKEFFPENRVEFFVSYYDYYQPEAYIPGR
                     DIYIEKDADINEVLEKMRLSTLKSVLTRRDVVVIASVSAIYASGDTRDFQG"
BASE COUNT          832 a          399 c          499 g          712 t
ORIGIN      
        1 agcttttatt tgtttttatt tttttggtgg ctttatgctt aactggtttt tctgcggatc
       61 agaggaaaac tcttttggta tatacaaaag gctatgctcc ttccacaggt cttgttgaaa
      121 ctcaggttaa ccaagagata gtgagtttgg gaaagtatag gctcgtcaca agggacgatg
      181 ttgttttcag agaaatttcg atgcagcttt caggtattct tgaaggtgga aatgcaaaag
      241 aaattcaaca gctgaaagct gacgctgtta tattcataca ggttaatgat gaaaacggct
      301 atcagaaatt cagtaaagac ggcaaaacat cgtgggtaga ctacgagata aataccaact
      361 acaaacttat tgatgtagcc tcgggacagg tcatgacagt caaaactttt gtcggtacag
      421 ggagcagtaa tataactcct aatgttcctt attctcaggc aatgaaaaac gctaagtata
      481 acgcagtata taatgtggca aatgagatag taatggaact caatacgctt tttaagataa
      541 aaggctctat cgtcaatata acctctgacg gaagagtaat aataaacgtc ggaaaagata
      601 tcggtgtata tgaaggaatg gtttttcaat ttgcaaaaga tgtcacaatt aatgaagata
      661 agtttgatac agtacaggga aaacttcttg caacagatgt aaagagcagc acctcgattc
      721 ttaagattct tgaactgcca aaggatttcg ctgtttcaga ccagggggta tacataactg
      781 aaactcctaa tatgtccgca ttaaggtcaa ggcttgcctt aaatggagga atgtatggaa
      841 taaaataccc tgtttttagc tttgacgtta ttgtagataa ctatcagggc cttgcttttg
      901 gaatgaacat tggagcatgc ttggcaaata taaactccaa aaacgttatt cttctggata
      961 caggatttat gcttggatac atgatagatc tggatgcaat ggaagcaacc attccggtac
     1021 agttcggaat gctttacgat ctttcaacag atgatataga taaagcagct ttttctttat
     1081 atataaaacc aggtctaaag atcgcagtca atctgggaga ttttatgggt gcatatgctc
     1141 agggcggcta catttttgaa attcctatga acagcattaa gacaaacgtc aacggaatta
     1201 cgcttcaggc aggagtcgag tttaaatttt aaactaaagt aaatcatagc cgcccaaaag
     1261 ggcggtttct ttatgatata atagttatac tattatactt atgtttatca ttatgaggtg
     1321 atgcagttga taaaaggagt aatatttgac ctttttggaa ctctgataaa ggtagaatct
     1381 ttatttttag gtattgcagg tgaaattgcc acacagaccg gaaacgaagt ccatgaaatc
     1441 gaaaaaaaaa ttcatacgct ttacaacaag cactttctta actatcattt aaaggagttt
     1501 caaccggaaa gacattacta tcaaaaggta ttcaaggagt taaaaaacga atataagctt
     1561 gcttattctg ccgactggta tgttgattat gcctatgata ccttttcaga acttagacag
     1621 tataatgatg ttaatttcct taatgacctc aaacaaagca actttaagat agctatactt
     1681 accaatgccg attctttttt cgtcagaaac tgtatagaaa aaaatgctat tgcctttgat
     1741 aagcttataa tatcagaaga agtaagagcc tacaaaccca gcgggcagat ctttcaaatc
     1801 gcacttgatg aagtaaagct gaaaaaagaa gaggttgttt tcgtaggaga taacataaat
     1861 gtcgatgtac ttggcggaaa caattttgga ataaaaacca ttcttataga cagagatgac
     1921 tcaaaagaat gctacccaaa agttacaaac ctctaccaga tcgctgaatt tataaggtga
     1981 gcatatgtat aaactaaaat caaattatat gccccaggga gatcagcctc aggccataga
     2041 cgatctcatg aatggtctta atagaaacga taggttccag actcttttgg gagtaaccgg
     2101 ctccggaaaa acatacacaa tggccaacgt tatagaaaaa tcaggaaggc cggctttgat
     2161 aatgtctcca aataaaatac tggctgttca gctttatagg gagttcaaag agttcttccc
     2221 ggaaaacagg gttgaatttt ttgtaagtta ttatgactac tatcagccgg aagcatatat
     2281 tcccggaaga gatatatata tagaaaaaga tgctgatata aacgaagttt tggagaagat
     2341 gagactttct actttaaaat ctgtactgac gcgcagagat gtagttgtaa tagcaagcgt
     2401 atctgccata tatgcatcgg gtgatactag agactttcag gg
//
LOCUS       DAOSHR010000271         2434 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_42252_length_2434_cov_3.358974, whole genome shotgun sequence.
ACCESSION   DAOSHR010000271 DAOSHR010000000
VERSION     DAOSHR010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42252_length_2434_cov_3.358974"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1819)
                     /locus_tag="PLU28_10590"
     CDS             complement(<1..1819)
                     /locus_tag="PLU28_10590"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG35548.1"
                     /translation="MTGKFFIRSLLIVSVMFIAGIIFIVLLNKNLSEQEVLTVNKAGF
                     SLASKIEDRVNRPLSAAYTLAAIIKTYGGFSSQQEFESIAQKITELYDGVSFLAAAPN
                     AVTSFIYPLHGNEKAIGHDLLNDPKRRDDVLRTIQSRSLTLSGPYELVQGGVSILGRL
                     AVFRDTDETESNFWGLAVVSIDLSKLLYDLELDSILDSGFLYEITRKDSPSDQEKIIL
                     TSIGEEYDKKKYAFSLNLPNSFWYLYIAPKKNRAYDILAWEVLILFIVLLVAFTIAYK
                     LLKQTYDIKTANMELHMQIKEKEKIELELIAAKKNADTANNAKSLFLANMSHEIKTPL
                     NGIMGFISLLNKTALDKEQKELVKQAEISGRSLKSLISDILDISKIEADKIELNTEPS
                     DIRNLIDEVVTIFLPLVKEKNIRLSVDISKDTPKVLDLDTGRLKQILINLIGNAVKFT
                     EQGEVSLSVRTQETTENNHRLLFSIKDSGIGIAENDFEKLFKSFSQVDSSTSKKYGGT
                     GLGLAISKKLIELMGGVITFKSTKGYGSTFEFFIPVRTSPGQIELKKHDSDVNNNTNN
                     SMSSDLKILVAEDNPINQKLISHILLKNGFDYELVSNGFQ"
     gene            complement(1816..>2434)
                     /locus_tag="PLU28_10595"
     CDS             complement(1816..>2434)
                     /locus_tag="PLU28_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35549.1"
                     /translation="FIIKRRNSLYNCGFLFDRAGKVVLEQMKTHLTPSEEKMGITCGN
                     TLETFLINGVRFAFPICMDASYFEVFRILMDMNTDVAVIPIANNEEYDISRAARGIWA
                     RVQETPMYGIKSSLNGWIMGQKFTGKAGIFAPVNLTASQNGVVSISSAPSGDFLITSL
                     IDPSSIRLYSKNNEYSVHHNFDFEFQSCNSYKKYNPHDIKKKGPA"
BASE COUNT          688 a          471 c          454 g          821 t
ORIGIN      
        1 cttgaaatcc atttgaaacc agttcgtaat caaatccgtt ttttaaaagt atatgcgata
       61 tgagcttctg atttatggga ttgtcttcgg ccaccagtat tttaagatca gaagacatac
      121 tattattagt attgttgtta acatcagagt catgtttttt aagctctatc tgaccgggag
      181 aagtgcgtac cggtataaaa aactcaaatg tggagccata tcctttcgtg ctcttaaagg
      241 taattacccc acccatgagt tcaatcagtt ttttggatat ggcaagtccc agtccggtgc
      301 cgccatattt ttttgatgtc gaagagtcaa cctggctgaa gcttttgaaa agtttttcaa
      361 aatcattttc tgcaattcct atcccgctgt cttttatgct aaagagaagt ctatggttat
      421 tttctgtagt ttcctgggtt ctgactgata atgaaacctc cccctgttct gtgaatttga
      481 cggcatttcc aataagatta ataagtatct gcttaagtct gcctgtgtcc aggtcaagaa
      541 ctttgggagt atctttactt atatctactg acagccgtat gtttttttcc tttaccaaag
      601 gaagaaatat agttacaacc tcgtctatca gatttcttat atctgacggt tctgtgttca
      661 attctatttt atcagcttca atctttgaaa tatcaaggat gtcagatatc agactcttca
      721 aggaacggcc tgaaatctct gcctgcttta ctaattcttt ctgttcttta tcaagagcag
      781 ttttattcag aaggcttata aatcccatta taccgttaag aggagttttt atttcatgac
      841 tcatattggc taaaaaaaga cttttggcat tgttcgcagt atctgcattt ttctttgccg
      901 caataagctc aagttcaatc ttctcttttt cttttatctg catatgaagc tccatattgg
      961 ccgtttttat atcgtatgtc tgctttaata atttgtaagc tattgtaaag gctaccaaca
     1021 aaacgatgaa taaaattaaa acttcccatg ctagtatatc gtaagctcgg ttttttttag
     1081 gggcaatata caggtaccaa aagctgtttg gaaggttcag actgaaagca tacttttttt
     1141 tatcatactc ttctcctata gacgttaaga tgattttttc ctgatcagaa ggagaatctt
     1201 tccgggttat ttcataaagg aacccgctgt caagtataga atcaagttca agatcataaa
     1261 ggagcttgct tagatctatt gataccacgg ccaatcccca gaagttcgac tctgtttcat
     1321 ctgtatctct gaatacagcg agtcttccca aaattgatac gcctccctgt accagctcat
     1381 aaggtccgga aagagttagt gaccggcttt gaatagttct taatacatca tcccttcttt
     1441 tgggatcgtt gagcagatca tgtccaatag ccttttcgtt tccgtgcaga gggtatatga
     1501 aagatgttac agcgttgggt gcagcagcga gaaaagatac tccgtcatat agttcggtta
     1561 ttttttgagc aattgattca aactcttgtt gagagctgaa acctccgtat gtttttataa
     1621 ttgccgccaa agtataagca gccgaaagcg gtctgtttac acgatcttcg atttttgaag
     1681 caagagaaaa tcctgctttg tttaccgtca ggacttcctg ctctgaaagg tttttgttta
     1741 aaagaactat gaaaataatg cctgcaataa acattaccga tactatcaaa agagatctga
     1801 taaaaaattt tcctgtcatg ctggtccttt ctttttgata tcatgcggat tatatttttt
     1861 ataggagttg cagctttgga attcaaaatc aaagttgtga tgcacagaat attcgttatt
     1921 ttttgaataa agccgtatgg aagaaggatc gataagggaa gtaatcagaa agtcacctga
     1981 gggagcactg cttatggaaa ctacaccatt ctgagaggca gtcagattaa ccggtgcgaa
     2041 tattcctgct tttccggtaa atttctgtcc cataatccaa ccgttcagac ttgattttat
     2101 accatacata ggagtttcct gaacccttgc ccaaatgccg cgtgccgccc ttgatatatc
     2161 gtattcttcg ttattagcta tcggaataac agcaacatcc gtattcatat ccataagaat
     2221 tcggaaaact tcaaaataag atgcgtccat acatatggga aaggcaaagc gtacgccgtt
     2281 tatgagaaat gtttcgaggg tattaccaca ggttatgccc attttttcct cagaaggagt
     2341 taaatgtgtt ttcatctgtt caagaaccac tttgccggct ctgtcaaata aaaatccgca
     2401 gttatacaga ctgttcctgc gttttattat gaag
//
LOCUS       DAOSHR010000272         2420 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_42618_length_2420_cov_3.415645, whole genome shotgun sequence.
ACCESSION   DAOSHR010000272 DAOSHR010000000
VERSION     DAOSHR010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42618_length_2420_cov_3.415645"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1778
                     /gene="lon"
                     /locus_tag="PLU28_10600"
     CDS             <1..1778
                     /gene="lon"
                     /locus_tag="PLU28_10600"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOG35550.1"
                     /translation="QKRLEDELEILTREIELLELEENLESKVKEKIEKTQKEFYLREK
                     LKTIQQELDGGTDEEISELKQKLLNQEYPKYVKEKAEKEIEKLSKMSPYSPESTVVRN
                     YLDWILNMPWNNETKDSIDIKIAKKILDSNHYGLSEPKERIIEFLAVKKLSNMAKTPI
                     LCFVGAPGVGKTTLGKSIAESLGRKFGRISLGGCRDEAEIKGHRKTYVGAMPGRIIQT
                     IRKLEVNNPVIVLDEIDKMGISYQGDPAAALLEVLDPGQNATFSDNYLEIPFDLSRVI
                     FVTTANVAHTIPPALLDRMEMLEIAGYTDFEKYNIAKNYIIKNTLPQNGLNPQEVQIT
                     PNALKSLISMYTREAGVRDLERTVSRLMRKVAVKYVKNRKQVVVKEDELKEYLGTPPF
                     FESEKNDRSESGLVTGLAWTAYGGSILNVEALTFPGKGNLILTGKLGEVMQESAKISL
                     SLCRRILSTMNIVDEKYFEKNDFHIHLPEGAVPKDGPSAGVTLTTALVSAISNKKVRN
                     EVAMTGEISLRGKVLAVGGIKEKLLSAYRAGIYEILLPFSNQKDVEKLPPEVIKKMKL
                     HFVRDIEQVLEIALIGGIKDAGKEG"
     gene            1759..2343
                     /gene="yihA"
                     /locus_tag="PLU28_10605"
     CDS             1759..2343
                     /gene="yihA"
                     /locus_tag="PLU28_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HOG35551.1"
                     /translation="MQARKVEFIKSVFSKNDYPQPLNGEIAFAGRSNVGKSSLLNALL
                     GIQIAKVSQVPGKTRAINYFLIDQKYYFVDLPGYGYARVSKEEKEKWNTFLTEYFYER
                     KSLQMVFLLIDHRHEVQANDISMVQWLKDIRVPFSLILTKSDKLSRSQQLKMTETIKS
                     QLSFYGEYLYFPTSAETRDGIKELNSYISNIFGE"
BASE COUNT          851 a          408 c          515 g          646 t
ORIGIN      
        1 ctcagaaaag actcgaggat gagcttgaaa tactgacaag agaaatagag ctcctggagc
       61 ttgaggaaaa tttagagtca aaagtaaaag agaagataga aaaaactcaa aaagagtttt
      121 atcttcggga aaaattaaaa accatacaac aggaacttga cggaggtaca gatgaggaga
      181 tctctgaact aaagcaaaag cttttgaatc aggagtatcc aaagtacgtt aaagaaaagg
      241 cagaaaaaga aatagagaag ctttcaaaga tgtccccata ctctcccgaa tcaacagttg
      301 taagaaatta tcttgactgg atacttaata tgccatggaa taacgagacc aaagattcaa
      361 ttgacataaa aatagcaaaa aaaattctgg attccaatca ttatggtctt tctgaaccaa
      421 aggaacgaat aatagaattt cttgccgtaa agaagctttc taatatggca aaaactccaa
      481 ttctttgttt tgtaggtgca cccggagtcg gaaaaacaac tttgggaaag tccatagcag
      541 agtcgttagg aaggaagttc ggaagaatat cgcttggagg atgcagagat gaagcagaga
      601 tcaaaggaca cagaaaaact tatgtaggag ctatgcctgg taggataatc caaactataa
      661 ggaagttgga agttaacaat ccagtgatag tattggatga aatagataag atgggtattt
      721 cttatcaggg tgatcctgct gccgcacttt tggaagtcct tgatcccggt cagaacgcaa
      781 ccttttcaga taattacctt gaaattcctt ttgacctttc acgggtgatc tttgtaacca
      841 ctgccaatgt tgcacatacc attcccccag cgctgcttga caggatggaa atgcttgaaa
      901 ttgcaggtta tacagatttt gaaaaatata atattgcaaa gaattatata attaagaaca
      961 cacttccaca aaacggactt aatcctcaag aggttcagat aacgcctaat gctctcaaat
     1021 ctctgatatc aatgtatact agagaagccg gagtgaggga tctggaaaga actgtttcca
     1081 ggctgatgag aaaagtggct gtaaagtacg ttaagaaccg gaaacaagtt gtagtcaagg
     1141 aagatgaact taaagagtat cttgggactc ctccattttt tgaatccgaa aaaaacgaca
     1201 gatccgaaag cggacttgtt acaggtcttg cgtggactgc ttacggtgga agtattttaa
     1261 acgttgaagc ccttactttt cctggtaaag gaaatcttat actcacaggc aaacttgggg
     1321 aagttatgca ggaatccgca aaaatttctc tcagcctatg ccggagaatt ttgagcacta
     1381 tgaacatagt agatgaaaag tattttgaaa agaatgactt tcacattcat cttcctgaag
     1441 gagctgtgcc aaaagatgga ccatcagccg gagttacact tacaacagcc ttggtttctg
     1501 caataagcaa taaaaaagtc agaaatgaag tagcaatgac aggagagata agtctcagag
     1561 gaaaagtgct tgcagttgga ggtataaaag aaaaacttct atccgcttac cgtgcaggta
     1621 tatatgagat tcttcttcca ttttcaaatc aaaaagatgt tgaaaaactg cctcctgaag
     1681 ttattaaaaa gatgaagctg cactttgtac gagatattga acaggtgctt gaaatagctt
     1741 tgataggagg tatcaaagat gcaggcaagg aaggttgaat tcataaaatc agttttttca
     1801 aaaaatgatt atccccaacc gcttaacggc gagattgcat ttgctgggag atcaaacgtt
     1861 ggcaaatcaa gtcttcttaa tgcacttttg ggtatccaga ttgccaaggt cagccaggtt
     1921 cccggaaaaa ccagagcaat aaactatttt ttgatagatc agaagtatta ttttgtagat
     1981 ctgcctggat atggttatgc cagagtttca aaagaagaga aagagaaatg gaatactttt
     2041 ctcacagagt atttttatga acgcaaatcc cttcagatgg tttttctgct tatcgaccac
     2101 agacatgagg ttcaggctaa cgatatttct atggtacaat ggcttaaaga tattagggta
     2161 ccgttttcat tgattcttac caaatctgat aaacttagca gaagccaaca gttgaaaatg
     2221 actgaaacta tcaaatcgca gctttctttt tacggggagt atctgtactt tccaacatct
     2281 gctgaaacca gagacgggat aaaagagtta aacagctata tttcaaatat ttttggagaa
     2341 tgaaaaatcg gactttttga agtggacccc tttagttaga caaaatctga cgaaaggggt
     2401 tttgttatgt caagaaaaag
//
LOCUS       DAOSHR010000273         2406 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_43006_length_2406_cov_4.877074, whole genome shotgun sequence.
ACCESSION   DAOSHR010000273 DAOSHR010000000
VERSION     DAOSHR010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43006_length_2406_cov_4.877074"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            142..621
                     /locus_tag="PLU28_10610"
     CDS             142..621
                     /locus_tag="PLU28_10610"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HOG35552.1"
                     /translation="MFLDNVALGRYVEKRSFMHNLDPRVKLLGLFVLAGFAFSINSVF
                     DIISMSVYTVILMMLSGLSLKYYLKSMKSIWFLVLFAFVMQLFSYEGTPVFSFWFITI
                     TDKALFNSMVVTFRLLFAILISTVLTLTTSPTSLAHAMEDILKWFFVPKSLHMNCLW"
     gene            615..932
                     /locus_tag="PLU28_10615"
     CDS             615..932
                     /locus_tag="PLU28_10615"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HOG35553.1"
                     /translation="MVMTIAIRFIPVMANEADRIIKAQMSRGANFDDRKISGKIKGIV
                     SIIIPLLISAMRRADDLSIAMEARGYHGWEGRTRFKVFSWKLSDTFFLFTFIAISVLI
                     TIY"
     gene            complement(926..1603)
                     /locus_tag="PLU28_10620"
     CDS             complement(926..1603)
                     /locus_tag="PLU28_10620"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOG35554.1"
                     /translation="MHPDILQISKLSLFNGIDTGELIDIFEESRFIYSSYEPGRVIKF
                     RGEKIEDLMILLYGDVKTEMGDFNGKAVQVEKLQAPFILASGFLFAQNNSLPVDITAV
                     SKATLISMKKQRVTEMCIKHKKILDNLLTDIGNRMEFLSEKLWLSTLKSLKEKFCFYI
                     LRLYNQQKTNPVILTDTLEEISAVMGVARPSLSRVIIEMEEEGLLKKSSNSIFIADKD
                     RMNAYFQ"
     gene            1697..>2406
                     /locus_tag="PLU28_10625"
     CDS             1697..>2406
                     /locus_tag="PLU28_10625"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HOG35555.1"
                     /translation="MSELFNKKEYLKNLILRVKDDGNDTQDIKKELKKTISELSPLEI
                     SQVEQELIENGQIDVSTIQSVCNVHLQMFKEYIENERIDLPDWHPINLLMKEHAFMMT
                     AADGIRDCSAKLRDKENIKDAIEIFLELNKFINHLADIENYFLKEENILFPFMEKYGV
                     KQPPAVMWKEHDQIRKIRKELSDMKNSRDLSQAKQKLEQLSIQLSEIVSMHIYKEHSV
                     LFPTALKLISEDEWKEIRT"
BASE COUNT          775 a          464 c          417 g          750 t
ORIGIN      
        1 ctcaccccct ttaatattat tttaccatat aattacattt cacgcaacaa aaaaagtcat
       61 tttttttgta aaaaaaacta tctgattttt catataatta tattataatt gcttatatac
      121 tttagaaagg cggtagataa agtgttcctt gacaatgttg ctttaggaag atacgtagaa
      181 aaaagatctt tcatgcataa tctcgaccca cgggtaaagc ttctggggct ttttgtactt
      241 gctggttttg ctttttccat caatagtgtt tttgatataa tttccatgtc ggtatacact
      301 gtaattctta tgatgctctc tggtctaagc ctgaaatact atcttaaatc catgaaatcc
      361 atatggtttc tagtgctttt tgcctttgta atgcagcttt tttcatatga aggtactccg
      421 gttttttctt tttggtttat tactattacc gataaagctc ttttcaattc aatggtagtt
      481 actttcagac tgctttttgc aatacttata tccacagtac ttacacttac aacatctccg
      541 acatctctcg cacatgccat ggaagatata cttaagtggt tctttgttcc taagtccttg
      601 cacatgaact gtctatggta atgaccatag ctataaggtt tattcctgta atggcaaacg
      661 aggcagacag gattataaaa gcacagatga gcagaggtgc aaactttgat gacagaaaaa
      721 taagcggcaa gataaaaggc atagtatcga taataatacc tttacttata tcggccatga
      781 gaagagccga cgatctgagc atagcgatgg aagcccgagg ctatcatggc tgggagggac
      841 gtacaagatt caaagtgttt tcatggaaac tttcagatac atttttcctt tttacattta
      901 ttgctataag cgttcttata actatttact gaaaatatgc gttcattctg tctttatcag
      961 caataaatat actgttgcta gactttttaa gcagaccctc ttcttccatt tctattatga
     1021 ctctggaaag agaggggcgt gccactccca ttacagctga aatttcttca agtgtatccg
     1081 taagtattac cggattagtt ttctgctgat tataaagcct gagaatataa aaacaaaact
     1141 tttctttcaa tgacttcaat gtgctcagcc ataacttctc agacaaaaac tccattctat
     1201 ttcctatgtc agtgagcagg ttatccagaa tttttttatg tttaatgcac atttccgtga
     1261 ctctttgctt tttcatgctt atcagtgttg ctttggaaac tgctgttata tcaacaggca
     1321 atgaattgtt ttgggcaaac agaaagcctg aagcaagtat gaaaggcgcc tggagtttct
     1381 ccacctgaac ggctttaccg ttaaaatctc ccatttctgt cttgacatca ccatagagga
     1441 gtatcatcag atcttctatt ttttcacccc tgaactttat aacccttcca ggttcataag
     1501 aactgtagat aaacctgctt tcctcaaaaa tatcgattag ctcacccgtg tcaataccat
     1561 tgaagagact taatttcgat atctggagaa tatccggatg catctgctgt cacctccgta
     1621 acaattgtta ccgactttcc ttctctcttt tattatactt aaaatgaact gaaaataaaa
     1681 ataaagaggt gaaagtatga gcgagctttt caataaaaaa gagtacctta aaaaccttat
     1741 actcagagta aaggatgatg gaaacgatac ccaggatatc aaaaaagagc ttaaaaaaac
     1801 cataagcgag ttatcgcctc ttgagatatc tcaagttgag caagaactta tcgaaaacgg
     1861 acagatagat gtttctacta tacagagtgt ttgtaacgtc catctgcaga tgtttaaaga
     1921 gtatatagaa aatgaaagaa tagatcttcc agattggcac cccataaacc ttctaatgaa
     1981 ggaacatgcc tttatgatga cagctgcaga cggcataaga gactgctctg ccaaactcag
     2041 agacaaagaa aatataaaag atgccattga gattttttta gagctgaaca aattcataaa
     2101 ccatcttgca gatattgaaa actacttcct aaaagaagaa aacattttgt ttccttttat
     2161 ggaaaagtat ggcgtaaaac agcctcctgc ggtgatgtgg aaagaacacg accagataag
     2221 aaagatacgt aaagaactgt ccgatatgaa aaatagcaga gatctatccc aggccaaaca
     2281 aaagcttgaa cagctcagca tacagctgag cgaaatagta tccatgcaca tatacaaaga
     2341 acactccgtc ctctttccca cagcgcttaa actcatatca gaagatgagt ggaaggagat
     2401 acgcac
//
LOCUS       DAOSHR010000274         2399 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_43206_length_2399_cov_4.092577, whole genome shotgun sequence.
ACCESSION   DAOSHR010000274 DAOSHR010000000
VERSION     DAOSHR010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43206_length_2399_cov_4.092577"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..693)
                     /locus_tag="PLU28_10630"
     CDS             complement(94..693)
                     /locus_tag="PLU28_10630"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOG35556.1"
                     /translation="MNEYIIFRRMADHDHDVRSDVSKVFADAYYKEMQFFSKDKDKLK
                     NAFRHAFCPEAVFTAEISGEIVSILGCSNNKTRAMHIDENQMKKHFGFITGHLAYSFL
                     QKEFNTVLTLPDNTGYIECAGTIERARGRGIGSALLNYVIQNQPYQEFILEVTDTNHV
                     AYRIYKKMGFIEFNRKSENFSKIKGFNQRIYMRWSRQAV"
     gene            complement(733..1608)
                     /locus_tag="PLU28_10635"
     CDS             complement(733..1608)
                     /locus_tag="PLU28_10635"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOG35557.1"
                     /translation="MNKIWEKILVIVSISFLVSSGLFGQDIKITTADDNKKKFMDYGS
                     YFKNINGSSVFINADTGIYFIYNHELAEFQTAPNSSFKIISCLMGLESGVIDPDNSLL
                     EWDETVYPVIEWNKDMDYKTAFKTSAIWYYRKVLDMIGREYVQKTLNSLNYGNCDISC
                     WQGSMDNKVFPMIRTLKSINGFWQESSLKVSPLEQAEVMCKIFRDGMCFSEDNIQLVK
                     ETMLINNYENSIKIYGKTGSGIKDEKWTDAWFTGFFECKDKTMYFSVRLNEPGTGGQQ
                     AKEIAINIINSEFGG"
     gene            complement(1632..2168)
                     /locus_tag="PLU28_10640"
     CDS             complement(1632..2168)
                     /locus_tag="PLU28_10640"
                     /inference="COORDINATES: protein motif:HMM:NF025432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4256 domain-containing protein"
                     /protein_id="HOG35558.1"
                     /translation="MTSDEMISILKKRFEENMNRHMHLEWIDLMKRLENNADKMNSLI
                     EMEVTGGEPDIIFKDGGEYIFYDCSPESPIGRRNICYDKKGQEERVKKGVFPKGNAVD
                     MAYAMGIDLLTEEQYKQLQTLGRFDIKSSSWLKTPFEIRKLDGAIFGDCRYGRVFIYH
                     NSAPSFYSSRGFRGCIKI"
BASE COUNT          683 a          521 c          335 g          860 t
ORIGIN      
        1 aattgcatta tatcgttcac tatatcacct tttcttataa gtaaaatctt tttattattg
       61 attatgtata ctgtttcttc aaaggtttta tactcagacc gcttgccttg accaacgcat
      121 atatattctt tgattgaaac cttttatttt tgaaaagttc tcactttttc tgttaaactc
      181 tatgaaaccc atttttttat atattcggta tgcaacatgg ttagtatcgg taacctccaa
      241 tataaactcc tgatacggct ggttctgaat aacatagtta agaagtgcgg atcctattcc
      301 tcttccccta gctctttcaa tagttccggc acactcaatg taacctgtat tatcgggcag
      361 ggtaagtaca gtgttaaact ctttctgcaa aaaactatag gccagatgtc ctgtaataaa
      421 cccaaaatgt tttttcatct gattttcatc gatatgcatg gctctggtct tattgttgga
      481 acatcccaga atacttacta tttcaccact tatctctgcg gtaaaaacgg cttccggaca
      541 gaatgcatga cgaaaagcat tttttagttt gtctttatcc ttagaaaaaa attgcatctc
      601 cttataatag gcatcggcaa aaacttttga cacatcactt cttacatcat gatcatgatc
      661 cgccattcta cggaatataa tgtattcgtt catagaaatc ccccctccgt taatttctct
      721 tttcaataga gtttatcctc caaactcgct gttaatgatg ttaatagcaa tttctttggc
      781 ttgctgcccg ccagtcccgg gttcgttaag tctgactgaa aagtacatag tcttatcctt
      841 acattcgaaa aaacctgtga accacgcatc agtccatttt tcatccttta tacccgaacc
      901 tgtttttcca tatattttta tactgttctc ataattattt ataagcattg tttccttaac
      961 cagttggatg ttatcttcgc tgaagcacat tccatcacga aagatcttac acataacttc
     1021 agcctgctct aacggagaga cctttaaaga agattcctgc caaaaaccat ttatggactt
     1081 aagagttctt atcatcggaa atactttatt atccatgctt ccctgccagc aacttatatc
     1141 acagtttcca tagttcagac tattaagagt tttttgtaca tattcccgcc caatcatatc
     1201 aagcactttt cggtaatacc atatagccga tgttttaaag gcagttttat agtccatatc
     1261 tttattccat tcaataaccg gatatactgt ttcgtcccat tctaataagg agttatccgg
     1321 atctattact ccgctttcaa gacccatcag gcaagaaata attttaaaag atgagttggg
     1381 agctgtttga aattctgcca gttcatgatt gtagataaaa tatatccctg tatctgcatt
     1441 aataaagaca gaactcccat ttatattttt aaagtatgaa ccgtaatcca tgaatttttt
     1501 tttattatcg tcagctgttg ttatttttat gtcttgtcca aataaaccgg atgaaactaa
     1561 aaaacttata ctcactataa ctaaaatttt ttcccatatt ttattcatat gatcagcctc
     1621 tgttccagaa atcatatttt tatacatcca cgaaatcctc tgctgctgta aaaagaaggc
     1681 gcactgttgt ggtatatgaa tacacggcca taccggcaat ccccaaagat agctccgtca
     1741 agttttctta tttcaaaagg cgttttgagc cagctggaac tttttatatc aaatcttcca
     1801 agggtctgca gctgcttgta ctgctcttct gtcagaagat ctattcccat agcatatgcc
     1861 atatctacag cattgccctt tgggaacact ccttttttaa ctctttcttc ctgacctttt
     1921 ttatcataac agatatttct gcgtccaatc ggactttcag gggagcaatc gtaaaaaata
     1981 tattctccgc catctttaaa aataatgtcc ggctcacctc ctgttacctc catctcaata
     2041 agggagttca ttttatcggc attattttcc aaacgtttca ttaagtctat ccattcaaga
     2101 tgcatatgac gattcatgtt ttcttcaaaa cgctttttta gtatgcttat catctcatcg
     2161 cttgtcaaaa acatcacccc tttatgccaa aaaattcgga ctcttacctt tataatatca
     2221 taaaaaaaga ttttcggatt acttttttcc ggaaatcttt agttttaagt ttgaccaatt
     2281 tatatttcag caaagcacag ttatgatatt ttacccattt catatgcctg ctgcatggcc
     2341 ggagtatctt ttatatcccc ttcattccat gcatttattc cgtatatgat acccttttc
//
LOCUS       DAOSHR010000275         2378 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_43788_length_2378_cov_5.974602, whole genome shotgun sequence.
ACCESSION   DAOSHR010000275 DAOSHR010000000
VERSION     DAOSHR010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43788_length_2378_cov_5.974602"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="PLU28_10645"
     CDS             <1..270
                     /locus_tag="PLU28_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HOG35559.1"
                     /translation="KRFLEITKMPPAREDLMENPIFNDFFIKNPLSAEYAKYVKYAVP
                     PALITKTVEVQDELTVSLVEPLMYGIKAPQAALDEAVKKINKIIW"
     gene            284..1168
                     /locus_tag="PLU28_10650"
     CDS             284..1168
                     /locus_tag="PLU28_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOG35560.1"
                     /translation="MEKISILKKRRVMKGWGISLTYLGYSAIFWGYPFVWLVILTFSK
                     WRYAGSPKFTGIDNILRVLSDELFWKTFLNTLNFMLYYIPMVLILSLLFAVALSKIKY
                     MKSFIVLSFLVANISSGVAYSILFSKLFAENGPLNQTLYKAFGVTVPWFTNPQFAMLS
                     IAIIVVWKFIGYYGLILYAGVQAIPKSVYEAAELDGAGKLTRFFKITIPLINPSLVMV
                     TVMAITLAFGIFTEPYMITGGGPMRSTLSPMMLMYTTAFQKLDPTYSATMSIFVALMS
                     FTIVFVFRKLFEREVNFQ"
     gene            1165..1995
                     /locus_tag="PLU28_10655"
     CDS             1165..1995
                     /locus_tag="PLU28_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOG35561.1"
                     /translation="MTKKSNRNFDLFINIVLILLSVLWLYPYIWLALSSFKPSNEIFT
                     GFFPSRLTLDHYRFIFETAQSMERPFFKALLNSIFVSGTVTASVILTSCIISFALTKY
                     EYKGGPFVINFIIFQMLLPGFMFTIPTYILIRNMGLLNSYSALILPSIMSGWGIFMIS
                     QSYKATPTDYIEAARIDGAKDIWIIFKIMMPLNKSAISIVGLFTFIGIWDNFMWPLIV
                     MKDYQKMPLSVLLASFNHEYGAYVGPVLAGAVMQTIPMVIIFILFRKYFLQGISMSLK
                     "
     gene            2014..>2378
                     /locus_tag="PLU28_10660"
     CDS             2014..>2378
                     /locus_tag="PLU28_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HOG35562.1"
                     /translation="MELKLTRHPLNPLFAPNPSHLWESRFVFNPAVVYDGELFHMLYR
                     AQGEDMVSRMGYAVSTDGIHFNRMEKPVFEPSCVQELYGVEDPRLTYIDGKYYMCYTA
                     YSPTNISVALASTKNFFNW"
BASE COUNT          707 a          484 c          457 g          730 t
ORIGIN      
        1 aaaagatttc ttgaaataac caagatgcct ccggcaaggg aagatctcat ggaaaatcct
       61 attttcaatg atttctttat taaaaatcca ctttcggcag agtacgcaaa gtacgtcaag
      121 tatgcagttc ccccggcact tattaccaaa acagttgaag tccaggatga actgacagta
      181 agccttgttg aacctcttat gtatggaata aaagcacctc aggcagcctt ggacgaagca
      241 gtgaaaaaga taaacaaaat aatatggtag ggaacgatta tttatggaaa aaataagtat
      301 actaaaaaaa cgcagagtaa tgaaaggttg gggaatttcc ctcacctatc tcggctattc
      361 ggcgatcttc tggggatatc cgttcgtctg gcttgtaata ttgacattct ccaagtggag
      421 atacgcagga agccctaagt ttacaggaat tgataatata ctgcgggttc tttcggatga
      481 acttttctgg aaaacctttt taaacacgct gaactttatg ctatattata ttcctatggt
      541 tcttattctt tcgctgcttt ttgcggttgc actctctaag ataaagtaca tgaaatcttt
      601 tatagtactt tcatttcttg tagcaaatat atcgtcaggc gtggcatact ctatactttt
      661 ttcaaaattg tttgccgaaa atggcccatt gaaccaaact ctctacaaag cattcggagt
      721 aacggtgcca tggttcacca atcctcagtt tgcaatgctt tcgatagcaa taatcgtggt
      781 atggaagttt ataggctatt acggactcat tctttatgca ggggtacagg ccataccaaa
      841 atctgtatat gaagccgcag agctggacgg agcaggaaag cttaccagat tctttaaaat
      901 aaccattccg cttataaacc cttctcttgt catggttaca gtaatggcta ttactcttgc
      961 tttcggaata tttactgaac catatatgat aaccggcggc ggtcctatgc ggagcacgct
     1021 ttcaccgatg atgctcatgt acactacggc atttcagaag cttgatccca catactcggc
     1081 aacaatgtct atctttgtag cccttatgag ctttacgata gttttcgttt tcaggaaact
     1141 ctttgaaagg gaagtgaact tccaatgacc aaaaaatcca acagaaactt tgatcttttt
     1201 ataaatatcg tacttattct attgtctgtt ttgtggttat atccttacat atggctggct
     1261 ctctcatctt tcaaaccatc gaatgagata tttacaggtt ttttcccttc cagactcact
     1321 cttgaccatt acaggtttat atttgaaaca gctcagtcaa tggaaagacc ttttttcaaa
     1381 gcattgctaa acagcatttt tgtatcgggc accgttacgg caagtgtaat acttacatca
     1441 tgcataatat cttttgcgct taccaagtat gagtataaag gtggcccttt tgtaattaac
     1501 ttcataattt tccagatgct cctgccgggc tttatgttca ccataccaac atatatcctt
     1561 atacggaaca tgggtctgct taactcatac agtgcgctta tacttcccag tataatgagc
     1621 ggatggggaa tttttatgat aagtcagagc tacaaggcaa ccccgacaga ttatattgaa
     1681 gccgcgcgga ttgacggagc aaaagatata tggattatct ttaaaatcat gatgcccctt
     1741 aacaaatccg caatatcaat agtcggactg ttcacattta ttggaatatg ggataacttt
     1801 atgtggcccc ttatagtaat gaaagattac caaaagatgc cgctttcagt cctgcttgca
     1861 agtttcaacc acgaatacgg ggcatatgtc ggtcctgtgc ttgcgggagc tgtcatgcag
     1921 acaataccta tggtaataat atttatacta ttcaggaagt acttcctgca gggaatttca
     1981 atgtctctaa aatagattta ggagggaatt tttatggaac taaaacttac aagacatccg
     2041 cttaatcctt tattcgcacc caatccgtca catctttggg aatcaagatt cgtatttaat
     2101 cccgccgttg tttatgacgg agaacttttt catatgcttt acagggcaca gggagaagac
     2161 atggtttcga ggatgggata tgcggtaagc actgacggaa tacacttcaa tagaatggaa
     2221 aaacctgttt ttgaaccgtc atgcgtccaa gaactttatg gcgttgaaga tccccgcctt
     2281 acctatatag acggaaagta ttatatgtgc tatacagcat actcaccaac caatataagc
     2341 gttgctcttg caagcaccaa aaactttttt aactggaa
//
LOCUS       DAOSHR010000276         2356 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_44369_length_2356_cov_4.180791, whole genome shotgun sequence.
ACCESSION   DAOSHR010000276 DAOSHR010000000
VERSION     DAOSHR010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44369_length_2356_cov_4.180791"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1638
                     /gene="infB"
                     /locus_tag="PLU28_10665"
     CDS             <1..1638
                     /gene="infB"
                     /locus_tag="PLU28_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOG35563.1"
                     /translation="EEVAQPEVSAQDDLEIKLKEKWEKIYKERRKDFRDRFPVVTVMG
                     HVDHGKTTLLDKIRNTKVAEKEAGGITQSIGAYQVGYKGKKISFIDTPGHEAFTEMRA
                     RGAQATDIVILIVAADDGVMPQTIEAYNHAKDAHVPVIVAINKIDKPNANVELTKQQL
                     ISKLNLVPEDWGGDTITVEISAKSGKGIDELLDMILLVAEMQEIKYYPKGNARAIIIE
                     SRLDKNLGPVATVIIKDGILKNGDFFVAGNTYGKVRRMQDDFGRTIKEATAPAPVLIQ
                     GFEDVPDMHSIFYAVDTLDEARSISQSKKLIEDNKRIGKKHIRLEEFMKLTDENKKKI
                     LNIILKSGTFGEIEALKTSIAKLKNPDVDLQVVHAGIGAVIPSDVMLASASDAVILGF
                     RVKADSKTLKLAEQEGIQVKRYEIIFNLIDDIKKALEGMLEPEEMEAVTGGGYIKELF
                     RIKKVGTIAGIQVTEGYVNKDGSVKLYRNGKFVTQVKIEQLKHYKDDVKSVDAPKECG
                     MKFFNFDDLVVGDELEFLKTVQVERKLEFDEQKENKK"
     gene            1645..>2356
                     /locus_tag="PLU28_10670"
     CDS             1645..>2356
                     /locus_tag="PLU28_10670"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG35564.1"
                     /translation="MPRFTGLLVYTGVIVLFYFLGLAVYILIWFTVSLFYPSFILPGP
                     GLVFSKLFVMLGDFSFWKSFLDTFLKLFVGFILSLGIGIPLGFFSGLSKKFYDFVRPT
                     LMFMQATPVVSYISIALLWFGIGFYTPVFICFVVIFPTVVINIAEGVGSTDRNLVMLS
                     RVFRLSKSDVIRYIYIPSIVPFINSTLKVVCGSLWRSVVIGEFMAGNRGLGYLLSLSK
                     STLRTDEVFANTLFLIIVG"
BASE COUNT          738 a          389 c          531 g          698 t
ORIGIN      
        1 gaagaagtgg cacaaccgga agtttctgct caggatgatc ttgaaataaa gcttaaggaa
       61 aaatgggaaa agatatataa ggaaagaagg aaggatttta gggacagatt ccctgtggta
      121 actgtcatgg gacatgttga ccacggaaag actactttac ttgacaagat tcgaaatact
      181 aaagtcgcag agaaagaagc agggggaatt acccagtcca taggtgccta ccaggtcgga
      241 tataaaggaa agaagataag ctttatagac actcccggtc acgaagcttt tacagaaatg
      301 agagccaggg gtgcacaggc aactgatata gttattctta tagttgccgc tgacgatggt
      361 gtcatgcccc agactattga agcttacaat catgcgaaag atgcccatgt tcctgttatt
      421 gttgctataa ataagattga taagccaaat gcgaacgttg aactgaccaa acagcagctg
      481 atatcaaaac tgaatcttgt tcctgaggac tggggaggag ataccataac ggttgaaatc
      541 tccgcaaaat caggaaaagg catagacgaa cttttggaca tgattcttct tgtcgctgaa
      601 atgcaggaaa taaaatatta tcctaagggt aatgcaagag ccattataat tgaaagcaga
      661 ctggacaaaa atctcggtcc tgttgcaaca gtaataatta aagacggtat tctcaaaaac
      721 ggagactttt ttgtagctgg aaacacctac ggaaaagtta gaagaatgca ggatgatttc
      781 ggtagaacca taaaggaagc caccgcacct gctcctgttc tcatacaggg gtttgaagat
      841 gttccggata tgcactctat tttttacgct gttgatactc ttgatgaagc cagaagtatt
      901 tcccagagta aaaagcttat agaagataat aagagaatcg ggaaaaaaca tattcgtctt
      961 gaagagttca tgaagctaac agatgagaat aaaaagaaaa ttctgaatat aatccttaaa
     1021 tccggcactt tcggagagat agaggctctg aaaacctcca ttgccaagct taagaatcct
     1081 gatgttgatc tgcaggttgt ccatgccgga ataggcgcag ttatcccaag cgatgttatg
     1141 cttgcatctg cctcagatgc tgttatccta ggttttagag tcaaagcaga ctctaaaact
     1201 cttaaacttg ccgagcagga ggggattcag gtaaaacgtt atgagataat atttaacctc
     1261 atagacgata taaaaaaagc tcttgaaggt atgttggagc ctgaagagat ggaagctgta
     1321 accggcggcg gatatataaa ggaacttttt agaataaaga aagtcggtac tattgccggt
     1381 atacaggtaa cagaaggtta tgtcaataag gacggaagtg taaaactata cagaaacgga
     1441 aagtttgtaa cccaggtaaa gatagagcag cttaagcatt acaaggatga tgttaaatcc
     1501 gttgatgctc caaaggaatg cggaatgaag ttcttcaact ttgatgatct tgttgtaggg
     1561 gatgaactgg aatttcttaa aaccgtccag gttgaaagaa aactagagtt tgatgagcag
     1621 aaagaaaata aaaagtaatc ttaaatgccc cgttttacgg ggcttttagt ttatacggga
     1681 gtgattgttc tgttttattt tttgggactt gcggtataca ttcttatctg gtttacggta
     1741 agcctttttt acccttcttt tatacttccc ggtcctggtt tggttttttc aaaacttttt
     1801 gtaatgctcg gagatttttc attctggaaa agctttttgg atactttttt aaagcttttt
     1861 gtgggattta tactgagtct tggaatcgga atacctttgg ggtttttttc aggtttaagc
     1921 aaaaaatttt atgattttgt acgtcctacc ttgatgttta tgcaagctac acctgttgta
     1981 tcttatatct ctatagcgct gctttggttc ggtatagggt tttatacacc tgtttttata
     2041 tgttttgttg tcatatttcc gacagtagtt ataaacatag cagaaggagt aggctcaact
     2101 gataggaatc ttgttatgct cagcagagtc ttccgtttat ctaaaagcga tgtaataagg
     2161 tatatctaca ttccttcaat cgtacctttt ataaactcta ctctgaaggt agtctgcggt
     2221 tcgttgtggc gttcggttgt gattggtgaa ttcatggcag gaaacagggg acttggttat
     2281 ttgttatcac tatcaaaatc gactctcaga accgatgaag tatttgccaa cactttattt
     2341 cttataatag tgggaa
//
LOCUS       DAOSHR010000277         2350 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_44560_length_2350_cov_3.899346, whole genome shotgun sequence.
ACCESSION   DAOSHR010000277 DAOSHR010000000
VERSION     DAOSHR010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44560_length_2350_cov_3.899346"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="PLU28_10675"
     CDS             complement(<1..220)
                     /locus_tag="PLU28_10675"
                     /inference="COORDINATES: protein motif:HMM:NF024194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HOG35565.1"
                     /translation="MLFSPKIDFVFKHMFASNDERSEILLKNFLNEVLGLKEGIKEIT
                     YLNPYNNKETQDDKISVLDLKIKDEESRE"
     gene            complement(315..>1218)
                     /locus_tag="PLU28_10680"
     CDS             complement(315..>1218)
                     /locus_tag="PLU28_10680"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOG35566.1"
                     /translation="LENIDTDFESIAIEEIKEKQQSAVISEEPKASQAKEDPSEEKEI
                     VSLNNEIPTLENIDTDFESIAIEEIKEEQQSTFDIENEKTNFIQARVEKLPGMVAVEK
                     NSFQMGDTLGDGRDNEKPSHTVTITYDYYIASYEVSFEEYGRYAEDVLKTKHKVFREN
                     SVVNVSWYEAIDYCNWLSKREGLSKAYSASGNFLDKNGRKTTDPSEVEGYRLPTEAEW
                     ESARSIISKNNDIWEWCSDWYSESYYEKSPQINPYNSSQEAFRVKRGGSLANNTNIRV
                     SCRSYDYPKHRREDVGFRIARTKT"
     assembly_gap    1219..1318
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1516..>2350)
                     /locus_tag="PLU28_10685"
     CDS             complement(1516..>2350)
                     /locus_tag="PLU28_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35567.1"
                     /translation="GFKDTEQFAISIQQQLLELYDAIEKKLLDLQTDPQELNNVSERL
                     NAVLMLKRKYGPSLNDVIANCGRFEEELNKLYELESIMNELTPRMEKIKKRLFEISES
                     IIKEAMPYLKEIKHGIEKNLVDLNMQNSEIDFYFEKLKEPGQISAHRIRMLMKTNPQS
                     DFLPLDKIASGGEMSRIFLAIETVFGNDHSVDSMLYDEVDAGVGPRMALTVGEKLKEL
                     SKEKQLIVITHMPQVANLAEKHLKITKVQNTDLVYSTVTELTGEQREKEIKDMYGEII
                     V"
BASE COUNT          544 a          471 c          352 g          883 t
ORIGIN      
        1 tctctcttga ttcttcatct tttatcttta gatccagtac tgatatttta tcgtcctgtg
       61 tttctttgtt gttgtacgga ttgagatatg ttatctcttt tatcccttct ttaagtccga
      121 gtacttcatt cagaaagttt ttaagcaata tttcacttct ttcatcattg cttgcaaaca
      181 tatgtttgaa tacaaagtct atcttcggtg aaaagagcat cgtccacctc cgcaatatta
      241 ttatatcatt tttttgtcca ttgttttagg tatacatcct tggagggatt aaatctccca
      301 tcaaggagag agttttaagt ttttgtcctg gcaatacgaa atcctacatc ttctcgtctg
      361 tgtttaggat agtcatagct gcggcaagat actcttatat tcgtattatt ggccaaacta
      421 cctccacgtt taactctaaa ggcttcttgg cttgagttat aaggattgat ctgcggagat
      481 ttctcatagt agctttcgct ataccagtcg ctgcaccact cccatatatc attattttta
      541 cttattatac ttctcgcact ctcccactct gcttctgtag gtaatctata tccttcaact
      601 tcacttggat cagttgtttt tctcccgttt ttgtcaagaa agtttccgga agcactgtat
      661 gcttttgaga gtccttctct ttttgaaagc cagttgcagt aatctatagc ttcataccag
      721 cttacattta caactgaatt ttctctaaat accttatgtt tagtttttaa aacatcctca
      781 gcatacctgc catactcttc aaagcttact tcataacttg ctatatagta atcataagta
      841 atcgttactg tatgagatgg tttttcgtta tccctgccat cccctaaagt atctcccatc
      901 tggaaactat tcttttctac agcaaccatt ccgggtaact tttcaactct cgcctgtata
      961 aagtttgttt tttcattttc aatgtcaaaa gtgctctgtt gttcttcttt gatttcttct
     1021 atagcaatac tttcaaaatc tgtatctatg ttttccaaag tcggaatctc attattcaat
     1081 gaaactattt ctttttcttc agacggatct tctttggctt gtgatgcttt tggttcttct
     1141 gaaattacag cgctctgttg tttttctttg atttcttcta tagcaatact ttcaaaatct
     1201 gtatctatgt tttccaaann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnct
     1321 ttttcttcag acggatcttc tttggcttgt gatgcttttg gttcttctga aatagttgtt
     1381 ttttctgttg agaatatctt gcttaatccg cttacaataa caaataataa aatgactaaa
     1441 aatattgcaa tctccatttt tgctcctttt tgttttatta gtatttgatc tgagccagtt
     1501 tttcaggaac tgagcttata caataatctc accgtacata tcttttattt ctttttcgcg
     1561 ttgttctcct gtcagctcgg tcacagttga atagactaga tctgtattct gtactttggt
     1621 gattttcaga tgtttttccg caagatttgc cacctggggc atatgagtta tgactataag
     1681 ctgtttttct ttggaaagtt ctttaagctt ctcgcctaca gtcagcgcca ttcttggtcc
     1741 tacacccgca tcaacttcat cgtaaagcat agagtcaaca gaatgatcgt ttccgaaaac
     1801 tgtttctata gctaaaaata ttctggacat ttctccgccc gaagcaatct tgtcaagagg
     1861 caggaaatcc gactgaggat ttgttttcat cagcatacgg attctgtgcg ctgatatctg
     1921 ccccggttct ttaagctttt caaagtaaaa atctatctct gaattttgca tattaaggtc
     1981 gaccaggttc ttttcgattc cgtgctttat ctctttcaga taaggcattg cttctttaat
     2041 tatactttcg cttatttcaa aaagacgttt ttttattttt tccattcggg gtgtaagttc
     2101 gttcattatg ctttcaagtt catacagttt attaagttcc tcttcaaatc tgccgcagtt
     2161 cgcaatgaca tcattcaaag acggcccgta ctttcttttc agcataagta ccgcattaag
     2221 ccgttcactc acattgttca gctcctgagg atcggtctga aggtctaaaa gttttttttc
     2281 tatggcatcg tataactcaa ggagctgctg ctgaatactt atggcaaact gctccgtatc
     2341 tttaaatccg
//
LOCUS       DAOSHR010000278         2345 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_44698_length_2345_cov_5.380349, whole genome shotgun sequence.
ACCESSION   DAOSHR010000278 DAOSHR010000000
VERSION     DAOSHR010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44698_length_2345_cov_5.380349"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="PLU28_10690"
     CDS             <1..695
                     /locus_tag="PLU28_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HOG35568.1"
                     /translation="ILGPELFDYGYGPFRWVCLSNDPKDLEKTDKAAMDCIDPNRRGQ
                     DRDNYIWIRDAQKNKLVVGSQARILYQDAAGRTRIALKFNQMVRNGEIGPVMMGRDHH
                     DTGGTDSPFRETSNIKDGSNVMADMATQCYAGNAARGMSLIALHNGGGVGIGKSVNGG
                     FGLLLDGSEKTDEVIKMAIPWDVMGGVARRAWARNQNSISTCMEYNQCSSSDHITLPY
                     IADEEKIRKLIK"
     gene            complement(745..1077)
                     /locus_tag="PLU28_10695"
     CDS             complement(745..1077)
                     /locus_tag="PLU28_10695"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HOG35569.1"
                     /translation="MQKDTGFFKQVYSIVSKIPQGKVATYGQIALILGKPRGARAVGW
                     AMRCAPQQLGLACHRVVNSKGTLSPFYVFGDCQIQKQLLSDEGITFTQDGRINMKKHL
                     WNFDSTVL"
     gene            complement(1089..1616)
                     /locus_tag="PLU28_10700"
     CDS             complement(1089..1616)
                     /locus_tag="PLU28_10700"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOG35570.1"
                     /translation="MNTLIKTENLILSTMNLKTVRLFMENNFDTQNILGWQCSPYWPT
                     IDIMTELFSMEKLLCSNPSHDIYGIWMVILRHNGVIIGDVGFRATPDINGTLTIDYGI
                     VPDQRNKGYGFEAVSTIFSFMLQNPAVSVVKAKCHCENIFSLKILYKIGMTVESQTGK
                     IISLSYVKNFIPRAC"
     gene            1922..>2345
                     /locus_tag="PLU28_10705"
     CDS             1922..>2345
                     /locus_tag="PLU28_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG35571.1"
                     /translation="MSVLSTKNLSKSFTVKIKKPGFKESLKSIFSPVMSEVNAVSNVS
                     FEVDEGEAVAFIGPNGAGKSTTIKMLTGILVPSSGEISVLGLCPYKDRLKLSYKIGTV
                     FGQKSQLWFHLPAHDTFDLMADIYEIDRVEYKYRLKRII"
BASE COUNT          746 a          413 c          502 g          684 t
ORIGIN      
        1 atatactcgg acctgagctg tttgattacg gatacggacc attcagatgg gtttgtctca
       61 gcaatgatcc caaagatctt gaaaaaaccg ataaagcagc catggactgt atagatccta
      121 acagaagagg tcaggacagg gataactaca tatggataag agatgctcag aaaaataagc
      181 ttgtagtcgg aagtcaggcc agaatacttt atcaggatgc agcgggcaga acaaggatag
      241 ctcttaagtt taaccagatg gtaagaaacg gtgaaatagg tccggtgatg atgggacgcg
      301 accatcacga tacaggcgga acagattctc cgttcaggga aacatcaaat ataaaagacg
      361 gttccaatgt aatggcggat atggcaactc aatgttatgc cggaaatgct gctaggggaa
      421 tgagtcttat tgctcttcat aatggaggag gagtaggaat agggaagtct gtcaacggag
      481 gttttggact tttacttgat ggaagtgaaa aaacggatga agtaataaag atggcaatac
      541 cgtgggacgt aatgggcgga gtagcccgaa gagcgtgggc aaggaatcag aactcaatat
      601 caacctgcat ggagtacaac cagtgcagtt catctgacca cattaccctt ccgtacattg
      661 ctgatgaaga aaaaataaga aagctgataa aataataaga aagccggata tccggctttc
      721 ttttatctat tcagagtatc ttttttataa gactgtagaa tcgaaattcc aaagatgctt
      781 tttcatattg attcttccat cttgcgtaaa ggtaattcct tcatctgaaa ggagctgttt
      841 ttgaatctga cagtccccaa aaacataaaa tggagagaga gtacctttgc tgtttacaac
      901 cctatggcac gcaagaccaa gctgctgagg ggcacatctc atagcccagc cgacagccct
      961 tgcacctctt ggttttccca agataagagc tatctgtccg tatgtggcaa ctttgccttg
     1021 aggtatttta gacacaatag agtaaacttg tttgaaaaaa ccggtatctt tttgcatggt
     1081 atcctccgtc agcatgcacg cggaataaaa ttttttacat acgacaagct tataatcttt
     1141 cctgtttggc tttcgacagt catacctatt ttataaagta tcttcagaga aaagatgttt
     1201 tcacagtggc attttgcttt tactacagaa actgccggat tttgcagcat gaatgaaaat
     1261 atagttgaga ctgcttcaaa accataacct ttgtttctct gatccggaac tattccgtaa
     1321 tctattgtaa gtgtgccgtt tatatcagga gttgctctga agcccacatc tcctattata
     1381 actccgttgt gacggagaat aaccatccat atcccgtaga tatcgtggga aggattggag
     1441 caaagcagtt tttccataga aaaaagctct gtcattatat ctatagtcgg ccagtatggg
     1501 ctgcattgcc agccaagaat attctgagta tcaaagttat tttccataaa aagccgtact
     1561 gtttttaaat tcatagttga aagaataaga ttttcagttt tgattagagt attcacaagt
     1621 cacttctttt ttcctgatag gagagtataa aaattctatc acataatgct taatcaaaaa
     1681 aatgaaaatg tataaattaa gttgactttt acattgtaat ttgttaaaat taatgaaaat
     1741 cttaaaaagg aaggatcgtt atgtttttat ctttaaccag tccgaatttt gcaaaagaac
     1801 ttaattttaa tatatgtgta atggtcatta tcaacagcgc cggcagccgc tgtgtctttt
     1861 gacatatccc aaattgccgg tgcttataag agcaccggtt tttatttata tgaggtgatt
     1921 tatgagtgta ttaagtacaa agaatctttc aaagagtttt acggtaaaaa tcaaaaaacc
     1981 aggtttcaag gaaagcctga aaagcatatt ttctcctgtt atgagtgagg tcaatgcagt
     2041 aagcaatgtt tcttttgaag ttgatgaagg agaggctgtt gcatttatcg gccccaacgg
     2101 agcaggaaaa tcaactacta taaaaatgct taccggaatt cttgtgccat caagcggaga
     2161 gataagtgtt ctgggcttat gcccgtataa agacaggttg aagctttctt ataaaattgg
     2221 aactgtattt ggacagaagt cgcagctttg gtttcatctg cctgcccacg atacgtttga
     2281 cctgatggct gatatatacg agatagacag agttgagtat aagtaccgtc taaagagaat
     2341 aattg
//
LOCUS       DAOSHR010000279         2339 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_44914_length_2339_cov_4.974168, whole genome shotgun sequence.
ACCESSION   DAOSHR010000279 DAOSHR010000000
VERSION     DAOSHR010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44914_length_2339_cov_4.974168"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="PLU28_10710"
     CDS             <1..645
                     /locus_tag="PLU28_10710"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOG35572.1"
                     /translation="KLGFRYSLATAKKILKEAGYTWNKEGKLLDKKGNIIKFELSTNA
                     GNVVREEISSIFVDSCAKIGIDITYRPINFNTLINNMNAGNYDSIVLGLTGGVDPGNG
                     WNVWRLDGGLHFWNYSPEAGDQFAWAKDRDYKVSDYEKRIDEIYKLQSVTVDDAKLKE
                     LFDEFQILVAKNQVVIYTIAQNYLAVFKNTVHIFNEKPSPAAGMLWKPWAVWKE"
     gene            711..1682
                     /locus_tag="PLU28_10715"
     CDS             711..1682
                     /locus_tag="PLU28_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG35573.1"
                     /translation="MLKYIIRRILLSIPVLLGVSVLAFMIISLAPGDFLDVYRLNPAI
                     SKAQLANLEKQFGLDKDPFTQYFLWLGNVLKGNWGYSFSYKVPVWNILGSRLEATLLL
                     SVTTFIFTWGIGIPLGIYSALHQYTLSDQLLSVFGFIGISVPSFFFSLLWLFMSAKTG
                     VFPVGGMIRNSFDQLPWFMKIIDYFHHVIGPVVTLGLGSLAGTMRIMRGQLLDELNKD
                     YVEFARAKGMPEKTVIFKHALRNAINPIVTALGFSISGLLGGAVFTETVFGWPGMGRL
                     VIEALNQQDLFLIMAVLLLSALLLVIGNLFADILLAAVDPRVRLRLS"
     gene            1702..>2339
                     /locus_tag="PLU28_10720"
     CDS             1702..>2339
                     /locus_tag="PLU28_10720"
                     /inference="COORDINATES: protein motif:HMM:NF024313.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG35574.1"
                     /translation="MSEEVKKNVPVNGKNPVDNNDGDIFEKEFLTATQLAWRTLRRHK
                     LGMVAMVTLIIFYFFAIFADFFSPMNPYNNHIQYRFAQPQKIRTKDFVTNERSGQYVL
                     LYDSVRDLYTKQKSFVEAAHYEAIKAYDINTEKEDLFELGKFNETYGATVTEIVSNFT
                     VTYKAKLKNGSYVELGTGTETEKPLIPVSYVTGDNITGQYEKDGNLLKPSFS"
BASE COUNT          733 a          422 c          495 g          689 t
ORIGIN      
        1 aagcttggct tcagatactc tttggctaca gccaaaaaaa tacttaaaga agccggatat
       61 acatggaata aagaaggaaa gcttcttgac aaaaaaggaa atatcataaa gtttgagctt
      121 tcaaccaatg ccggaaatgt tgtacgagaa gaaatttcaa gcatatttgt cgattcatgc
      181 gcaaaaatag gaattgatat aacttacaga cctataaact ttaatactct tataaacaac
      241 atgaatgccg gaaattatga ttctatagtt ctcggtctta ctggtggagt agatccgggt
      301 aacggctgga acgtatggag actggacggc ggactccact tctggaatta ctcacctgaa
      361 gcaggcgatc agtttgcttg ggcaaaagac agagattaca aggtatccga ttacgaaaag
      421 agaattgatg aaatatataa gctgcagtcc gtaacagtag acgatgcaaa gcttaaagag
      481 ctctttgatg aattccagat ccttgttgca aagaatcagg tagtaatata cactatagct
      541 cagaactacc ttgcagtatt caaaaacaca gtacacatat tcaatgaaaa gccgtcacct
      601 gcagcaggta tgctctggaa accatgggca gtttggaaag aataagcaaa tagaataaaa
      661 ggggcgggag ttacccactc ccgcccttta tttttaagga ggggaagata ttgctaaagt
      721 acatcatcag gaggatactt ctttctattc ctgtacttct tggagtatct gttttagctt
      781 tcatgatcat atctcttgca cctggagact ttttggatgt atacaggctt aatcctgcta
      841 tatcaaaagc tcagcttgcg aaccttgaaa aacagttcgg tttggataag gatcctttta
      901 ctcagtactt cttgtggctt ggaaatgttc tgaagggaaa ctggggatac tctttttctt
      961 acaaagttcc ggtatggaac atccttggat ccagacttga agcaacgttg ctgcttagtg
     1021 tcactacctt tatttttacc tgggggatag gaatcccgct gggtatatat tcggctttac
     1081 accagtacac tctaagtgat cagcttttgt ctgtatttgg tttcatagga atatcagtac
     1141 cgtcgttttt cttttcactg ctatggcttt ttatgtcggc aaaaaccggt gtgttcccgg
     1201 ttggcggtat gataagaaac agttttgacc aactgccgtg gtttatgaag attattgact
     1261 acttccatca cgtaatagga ccagtggtaa cccttggact aggaagcctt gccggtacta
     1321 tgcgtataat gaggggacag ttgcttgatg aactgaataa agattatgtt gagtttgcca
     1381 gagcaaaagg tatgcccgaa aaaacagtta ttttcaaaca tgctctcaga aatgctatca
     1441 accctatagt tacagcatta ggattcagca tatccggtct tttgggagga gctgtattta
     1501 ccgaaacggt ttttgggtgg cctggaatgg gaagacttgt aattgaagct cttaatcagc
     1561 aggatttatt cctgataatg gctgtactcc ttttgagtgc tttattgctt gttataggaa
     1621 acctatttgc agatatcttg cttgcggccg ttgatccgcg tgtaagatta agactttcat
     1681 aaggatacgg aggtaatcag gatgtcagaa gaagttaaaa agaatgttcc tgtcaacggt
     1741 aagaatcctg ttgacaataa tgacggagat atttttgaaa aagaattttt gacggctaca
     1801 caactggcat ggagaactct gaggcgtcat aagctcggaa tggtggcaat ggtaacactt
     1861 ataatttttt acttttttgc tatatttgcc gacttttttt ctccaatgaa tccatataat
     1921 aaccatatac agtatagatt tgcccaacct cagaagataa gaaccaaaga ttttgttacc
     1981 aatgaaagat ctggccagta tgtactctta tatgacagtg ttagggatct ttacactaaa
     2041 cagaaatctt ttgtagaagc tgcacactat gaagctataa aagcttatga cattaataca
     2101 gaaaaagaag acctttttga acttggaaag tttaacgaaa cttatggcgc aacagtaacg
     2161 gagatagtat caaactttac tgttacatat aaagctaagc ttaaaaacgg atcatatgtc
     2221 gaactcggta ccggaacaga aaccgaaaaa cccttaattc cggtatcata cgtcaccggg
     2281 gataatataa caggacagta tgagaaagac ggcaatcttt taaaaccttc tttttccaa
//
LOCUS       DAOSHR010000280         2318 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_45479_length_2318_cov_4.573133, whole genome shotgun sequence.
ACCESSION   DAOSHR010000280 DAOSHR010000000
VERSION     DAOSHR010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45479_length_2318_cov_4.573133"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="PLU28_10725"
     CDS             complement(<1..415)
                     /locus_tag="PLU28_10725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOG35575.1"
                     /translation="MSSRVRRDTARGFWFVLPAVAVIALVTFFPIARTFILSLYKYNL
                     KFPSMKAFVGVNNYINLFQNQDFLNSLTVTVSFTLVAVSIEAVAGLLIALLINKDFKG
                     RGMVRAFTLIPWAIPTVASAQMWRLMYSDQFGVIND"
     gene            complement(485..1729)
                     /locus_tag="PLU28_10730"
     CDS             complement(485..1729)
                     /locus_tag="PLU28_10730"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOG35576.1"
                     /translation="MKKLILVMLVAVMVLSLSFAKVKITYFGDNQAQSVDAILTQIFN
                     QSQDDIEVEFLLQSWSTDDKHTALVTRLGAKDKNPTVYLGDVIWPAEFGAAGWALDLS
                     PYMTKDELAKFIPGTIKSCTYNGKLYAIPAFTDGGLLYYRKDLLDKYGYKPPTTWDEL
                     IKIAQDVSKKENIHGFVFQANQYEGLVCDMVEYIAANNGEIINDKNEVKINTPEVIEA
                     MQFMQDMIHKYKIAPESVTTFGEEDARQVFQQGKAVFMRNWPYCNSITNLGSSESAVK
                     GKYGMVPMPRGPKGKAGAATLGGWNVFVNPFAPKDEKEAGVKFAKFLTSEVAQILRVI
                     VAGNSPTRVDVYTNAAALKANGDLTTYYPIMINAVPRPVTPYYAEITQILQKGFHSVV
                     TNKSKPADAAKQMEAEIKKVYQ"
     gene            complement(1763..>2318)
                     /locus_tag="PLU28_10735"
     CDS             complement(1763..>2318)
                     /locus_tag="PLU28_10735"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOG35577.1"
                     /translation="NRHKNFDSVYVDNFYGGQLAAKMLLENNCHDFFAIQTKETLNLM
                     VSGVMDERLEGFFNLLQEEKVSITEDNIVFSDLLIGPSTQKIAQILKKFRKPGIFALT
                     DTFALIVLQVAASLGKIPGKDFFLVGFDNQSWTNQYGLSTIGQPIEEMGARTAELALA
                     KISNPAMSVQHIKLMPVAIKRSSA"
BASE COUNT          652 a          507 c          453 g          706 t
ORIGIN      
        1 tatcgtttat tactccaaac tggtcggaat acataagcct ccacatctgt gcagaagcga
       61 ctgtcggaat tgcccaagga ataagagtga aggcacggac catccctctg cccttgaagt
      121 ctttgtttat aagcaatgcg atgagaagcc ctgcaacggc ttcgatactc acggcaacca
      181 gggtaaagga tacagtgaca gtaagagagt ttaaaaaatc ctgattctga aaaagattta
      241 tatagttgtt aacacctaca aaagctttca tactcggaaa tttgagattg tatttataaa
      301 gagaaagaat aaaagttctt gctatgggga aaaaggttac cagtgctatg acagctacag
      361 ccggtaaaac aaaccaaaac ccccgtgcgg tatctcttct aactctcgaa gacattataa
      421 tccctccgtt atttataaag aaacgctccc ttttatgcaa agggagacat ttcctttgtt
      481 tgaattattg gtatactttc tttatttcag cttccatctg ttttgcggca tctgcaggtt
      541 tactcttgtt ggtaacaaca gaatggaaac ccttctggag aatctgggtt atctcagcat
      601 agtacggagt aacaggtctg ggaacggcat ttatcattat aggatagtaa gttgtgagat
      661 cgccgttggc tttaagggcg gcggcattag tataaacatc gactctggtc ggagagttgc
      721 ctgctacaat taccctaaga atctgtgcca cttctgatgt caagaatttt gcaaacttaa
      781 ctcctgcttc tttttcatct ttaggagcga aaggatttac aaatacattc caccctccca
      841 aagtagctgc gccggctttt cctttcggac cccttggcat cggaaccatt ccatactttc
      901 cctttacagc tgattctgag ctgccaaggt tggttattga gttgcagtaa ggccagtttc
      961 tcataaaaac agctttaccc tgctggaata cctgtcttgc atcttcttcg ccgaaggttg
     1021 ttacagactc cggagctatc ttatacttat gaatcatatc ctgcataaac tgcatagctt
     1081 caataacttc aggcgtattt atctttactt cgtttttgtc attgattatt tcaccgttat
     1141 tggcggcaat atattcaacc atatcgcata caagtccttc gtactgattg gcctggaata
     1201 caaaaccatg aatattttct ttctttgaaa catcttgggc tatctttatc agttcatccc
     1261 atgtcgtcgg cggtttataa ccatacttgt caagaagatc ttttctgtag tacagaagac
     1321 ctccatcggt aaaagcagga atggcataaa gcttgccgtt gtaagtacat gattttatag
     1381 ttccgggaat aaacttggcc agttcatcct tggtcatata agggcttaga tccaaagccc
     1441 atccggctgc tccgaactcc gcaggccaaa tgacatcgcc aagataaacc gtaggatttt
     1501 tatcctttgc cccaagtctg gttacaagag ccgtgtgttt gtcatcagtc gaccatgact
     1561 gaagcaggaa ctctacttca atatcgtctt gtgactggtt gaaaatctgt gtaagaatgg
     1621 catctacgct ttgagcttgg ttatcgccga agtacgtgat cttaaccttg gcaaaagaca
     1681 atgaaagcac catgacagca accaacataa ctaaaatcag tttcttcacc tataacaccc
     1741 ccgtacagtt gatatgttct tttcatgcag aacttctctt tatagcaacg ggcatgagtt
     1801 ttatgtgttg aacactcata gccggattgc ttatctttgc aagcgcaagc tcagcggtcc
     1861 ttgcccccat ttcctctata ggctgaccta tggtcgaaag accatattgg ttggtccagc
     1921 tttgattatc aaaaccgact agaaaaaagt cctttcctgg gatttttcca aggctggctg
     1981 ccacctgaag aactataagg gcaaatgtat ctgtaagagc aaaaattccg ggttttctga
     2041 attttttcag tatctgagca atcttttggg tagacggtcc tatcaggaga tccgaaaata
     2101 caatattatc ttcagttata gaaacttttt cttcctgaag aaggttaaaa aagccttcaa
     2161 gcctttcgtc cattacaccg cttaccataa ggttaagggt ttctttggtc tgaatagcaa
     2221 aaaaatcatg acagttattt tccaaaagca tttttgcggc aagctgtccg ccataaaaat
     2281 tatccacata aacactgtca aagtttttgt gcctgttt
//
LOCUS       DAOSHR010000281         2316 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_45532_length_2316_cov_5.246351, whole genome shotgun sequence.
ACCESSION   DAOSHR010000281 DAOSHR010000000
VERSION     DAOSHR010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45532_length_2316_cov_5.246351"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..604)
                     /locus_tag="PLU28_10740"
     CDS             complement(<1..604)
                     /locus_tag="PLU28_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35578.1"
                     /translation="MNLINNELFKLHMKKRLLPFFILNFIADVLLISFVLIKKDSIFY
                     SLINGQNFPLFSLEITLVFSIVFSCMSVYDLFIYEDKSGTLCTELLTPLNRKEVVLSK
                     FFAIMLELEVFFAVNLIFSYTISAVFLEFGEVFSYCGTFLPFKEGLFKTVFSYVLIFF
                     PLSGVTSFAILLYFLISKKIKSPGFLFFMVLSLSIMGLFEL"
     gene            complement(620..1486)
                     /locus_tag="PLU28_10745"
     CDS             complement(620..1486)
                     /locus_tag="PLU28_10745"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG35579.1"
                     /translation="MSGSLLVQNIIKTRENKSILDNISFEVHQNESLGIFAKTGQGKT
                     VLSKILMTLVQIDSGKITINGYDIHQNFEKAICKTGSIVDSPKFYDELTAYQNLLLTA
                     NLYGDIPHRRAWEMLELTDLQKNKDHKVKSFTSAMKYRLAIARAMINYPWLLILDDPA
                     RDLDMQESLKISCILEQIRVNYSVSMLILSKSKEFLLKTCADMLCMENGKVFSFSPDK
                     HSLDLAEIIDIYSVQKAQITKILYSMPYVSEIQSKEFGLRIKAEKEESQRILDTLLAN
                     KVNIDYFLPSGT"
     gene            1635..2105
                     /locus_tag="PLU28_10750"
     CDS             1635..2105
                     /locus_tag="PLU28_10750"
                     /inference="COORDINATES: protein motif:HMM:NF024135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HOG35580.1"
                     /translation="MATLMVYASKHGSVEKSLNSMKKSIKDQTLMIPIKEYSNGMLDT
                     YRAVVIGCSVHAGTVQKEVVDFVKGNLQMLSTKKTVVVINCADQEKYTEYFENAFGKN
                     ILSENFSLVYGGFGYYFEKMGFLERTLIKMISKMKENKEFINEKGLRDAAAKLE"
BASE COUNT          793 a          378 c          421 g          724 t
ORIGIN      
        1 tcagctcaaa aagtcccata attgataatg ataaaaccat gaaaaaaaga aaacctggag
       61 actttatttt tttggaaatc aaaaaatata aaagtattgc aaagctggtt actccggaaa
      121 gcgggaagaa gataagaaca tatgaaaaaa ctgttttgaa cagaccttcc ttgaaaggca
      181 agaaagtacc acagtaagag aatacttcac cgaattccag aaaaacagcc gatattgtat
      241 atgaaaatat caggttgacc gcaaaaaaaa cctccagctc gagcattatt gcaaaaaatt
      301 ttgaaagaac aacctctttc ctattaagcg gtgtaagcaa ctcggtgcat aatgtcccgc
      361 ttttatcctc gtatatgaaa agatcataca cactcatgca agaaaaaaca attgaaaaaa
      421 ccaaggtgat ttcaagtgag aaaagaggga agttctgacc atttatcaag ctataaaaaa
      481 tgctatcctt ttttattaaa acaaaactta taagcagcac atcggcgata aaattaagga
      541 taaaaaaagg taacagcctt tttttcatat gcagcttgaa gagttcattg ttaattaaat
      601 tcattctgat tctcctgttt tatgttcctg aaggcagaaa ataatctata tttactttat
      661 tggccaacag ggtgtcaagt attctttgag attcttcctt ttcggccttt atgcgtaatc
      721 caaactcttt tgactgaatt tcagagacat agggcatcga gtacaggatt tttgttatct
      781 gtgctttctg aacagaatat atgtcaatga tttcagcaag atctaagctg tgcttgtcag
      841 gcgaaaagct gaaaactttt ccgttttcca tacaaagcat atcggcacat gtttttaaaa
      901 gaaattcctt gctttttgat agtataagca ttgagaccga ataatttact ctgatctgct
      961 ccaaaataca ggaaatttta agactttcct gcatatcaag atctcttgcc gggtcatcca
     1021 gtataagaag ccatggatag tttatcattg cccttgcaat tgcgagcctg tacttcattg
     1081 ctgaggtaaa agacttgacc ttgtgatcct tgtttttctg cagatcagtc agttcaagca
     1141 tctcccatgc tcttctgtgt gggatatccc cgtaaagatt agcggttaag agaagattct
     1201 gatatgcggt aagctcatca tagaattttg gagaatcaac aatacttcca gttttgcaga
     1261 tagctttttc aaagttctga tgtatgtcgt atccgttgat cgtaattttt ccggaatcta
     1321 tctgtaccag ggtcatgagg atctttgaaa gtacagtttt cccctggccg gttttggcaa
     1381 agattcccag tgattcgttc tgatgaactt caaaagatat attatccagt atacttttat
     1441 tttctcttgt ttttattata ttttgaacca gtagactccc ggacattact tttcctccat
     1501 tatattacta taattttagt gttctatatt agtataacac taaaacatga tattgtcaac
     1561 tcttaccgaa aaataattgt atgtgctata atttagccat gaacagaaga gaaaagcata
     1621 gaaggaggaa aaaaatggct acattaatgg tttacgcatc aaagcacgga agtgtagaaa
     1681 agtctcttaa cagcatgaaa aaaagtataa aggatcaaac ccttatgatt ccgataaagg
     1741 aatactcaaa cggtatgctt gatacttaca gggcggttgt aataggctgt tctgttcatg
     1801 ccggtactgt acaaaaggaa gttgttgatt ttgtaaaagg aaatcttcag atgctttcaa
     1861 ctaaaaaaac agttgtggtc ataaactgtg ccgaccagga aaagtatact gaatattttg
     1921 aaaatgcttt tggcaaaaac attctttctg aaaatttttc ccttgtttat ggaggatttg
     1981 ggtactactt tgaaaagatg ggttttttag aaagaacctt gataaaaatg atttcaaaga
     2041 tgaaagaaaa taaggagttc ataaatgaaa agggcctacg ggatgcagct gccaaacttg
     2101 aatagcttaa agacagaatc tttcatatat tattaagaag tatattaaaa agcgaaaaga
     2161 tgcttaaaat atttatgtta aaataatggt cggaactggc aaatatatct taaggaggaa
     2221 aaaaatgaaa aaagtgttta cagtgctact ttctttagtg attttggtag taggagtcat
     2281 tgcctcaaaa cctttatatt cgcttttcag tgatta
//
LOCUS       DAOSHR010000282         2305 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_45848_length_2305_cov_5.342667, whole genome shotgun sequence.
ACCESSION   DAOSHR010000282 DAOSHR010000000
VERSION     DAOSHR010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45848_length_2305_cov_5.342667"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1022)
                     /locus_tag="PLU28_10755"
     CDS             complement(<1..1022)
                     /locus_tag="PLU28_10755"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOG35581.1"
                     /translation="MQSLQPLVKELGAEIIVVDTGSTDSTVEIAKKYTDKLYFHEWNN
                     DFSGMRAVTVSYAKGEWIFIIDGDEVLQDAAEIIDFFRSGLYKEYKTAMLEVKNFSKK
                     DLSIYSLLGSPRLFRAGTFHYEGAVHNQIIHEFPGLKLISTMLLHYGYIAEDKELMER
                     KFERTSALLKKELEKNPNSVYYRYQLSTTYLMHGDVDKALEEGEKAYFMIKDDNDKVL
                     RYMYILGYIAKIYIKLERFEEMFEASEKGLSFNQEYLDLWFYNFYYFFNKKEHDKIIE
                     KGLKYIDVHENFKYLAMSRNSSVIMDTLDKIDLVRLFVARSFFEKKDYESVLKQIMLI
                     NPSFEF"
     gene            complement(1164..2300)
                     /locus_tag="PLU28_10760"
     CDS             complement(1164..2300)
                     /locus_tag="PLU28_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HOG35582.1"
                     /translation="MRLLDEAKAAKTLVGLSGAQTINTAGHYAAKFDVEGQAIGDLAK
                     LGTKLYDIDKFWDASGNFILENPATIELVQGDGTRTSITISKSDTIGDVKDKLNTAIG
                     TGLGQQDVTGVEAGNFVSFVEAATSSTTGLEAVNGTFVIRSAIAGSDGKITFVGDDRV
                     LAALSLTTIQKEQNNNYTVKVTEAHGGTVVAENVKISENNLIGIVHKNVDVQFAANTG
                     IKTSWDSTEKDFKLEGGASYKGTTYVHLADNTTVLHIGANQKQDIGTGIANMSARALG
                     VNNILVMNNTLANEAIGKLDKAINTVSSERAKLGAIQNRLDHTISNLTTTSENLTAAE
                     SRIRDVDMAKEMMNFTKYQILTNAATAMLAQANQLPQSVLQLLR"
BASE COUNT          593 a          513 c          421 g          778 t
ORIGIN      
        1 ttgaactcaa atgaaggatt aataagcata atctgtttca agacactttc atagtccttc
       61 ttttcaaaaa aagatcttgc cacaaaaagc cttaccaaat ctattttatc aagagtatcc
      121 ataataaccg aagagttcct gctcatggca agatatttga agttctcatg gacatctatg
      181 tatttaagtc ctttttcaat tattttatca tgttcttttt tattaaaaaa gtaataaaaa
      241 ttataaaacc acagatcaag gtactcctgg ttaaagctta agcctttttc ggaagcttca
      301 aacatctctt caaacctttc cagctttata tagatctttg ctatatatcc aagtatatac
      361 atatacctta ataccttatc gttgtcgtct tttatcataa agtaagcctt ttctccctct
      421 tcaagggctt tatcgacatc cccgtgcata aggtaagtgg tcgaaagctg atacctgtag
      481 tataccgagt tgggattttt ttcaagttct tttttcagaa gagccgaggt tctttcgaac
      541 tttctttcca taagctcttt atcctcagct atatacccat aatgcagaag cattgtcgat
      601 ataagcttaa gacccggaaa ctcatggatt atctgattgt gaaccgctcc ttcataatga
      661 aaggttccgg ctctgaataa gcgtggcgat ccaagcagtg aataaataga aagatctttt
      721 ttgctgaaat ttttaacctc cagcatagct gttttatact ctttataaag accgcttctg
      781 aaaaagtcta tgatctctgc ggcgtcctgg agaacttcat ctccgtctat tataaagatc
      841 cactcaccct tggcataaga cactgtaaca gctctcatcc ccgaaaaatc gttgttccat
      901 tcatgaaagt acaacttatc cgtatacttt ttggctattt caaccgtact gtctgtagaa
      961 cccgtgtcaa caactattat ctcagccccg agctccttga ctaaaggttg caggctctgc
     1021 aaacattcct caagatgtct ctcttcattt ttaaccatca tgcagatgct taacaacata
     1081 ccttcaccgt cctaaaagaa atcgttttat acgattatac tataaaaaga agcgggattc
     1141 ctcccgctcc tgcaaaacta ttattatctg agtaactgca gtacactctg gggcagctgg
     1201 tttgcctgtg ccaacattgc tgttgctgca ttggtaagta tctggtactt tgtaaagttc
     1261 atcatttcct tggccatatc tacatctctg attcttgatt ctgctgctgt caagttctcg
     1321 gatgtggtgg tcaggttgga tatggtgtgg tcaagtctgt tctggatagc tccaagcttt
     1381 gctctttctg aagaaaccgt gttaatagcc ttgtcaagct ttcctattgc ttcgttagcc
     1441 aatgtgttgt tcataacaag gatgttgttt actccaagag ctcttgcgct catgttggct
     1501 atacctgttc cgatatcctg tttctggttt gcaccgatat gaagaactgt ggtgttatcg
     1561 gccaagtgga catatgtggt acccttatat gaagctccgc cttccaattt gaaatctttt
     1621 tctgttgaat cccatgatgt ctttataccg gtattggcgg caaactgaac atcaacattc
     1681 ttatgaacta ttccgataag gttattctcg gatatcttta cattttctgc aacaactgtt
     1741 ccgccgtgag cttctgttac cttgactgta tagttgttgt tctgttcttt ctggattgtt
     1801 gtcaagctga gggctgcaag taccctgtca tcaccgacaa aggttatctt accgtctgaa
     1861 ccggcaatag ctgatcttat tacgaatgta ccgtttacag cctcaagacc ggttgttgag
     1921 gaagtagctg cttcaacaaa tgaaacaaag ttacctgctt ctacccctgt aacatcctgc
     1981 tgtcccaaac ctgttccgat tgctgtgtta agcttgtcct ttacatcgcc tatagtatcg
     2041 ctcttggaaa ttgttatgct tgttctggtt ccgtcaccct gtaccagttc gattgtagca
     2101 ggattttcga gtatgaagtt accgcttgca tcccagaact tgtctatgtc ataaagcttg
     2161 gttccgagct ttgcaagatc tccgatagcc tggccttcaa cgtcaaactt tgcggcatag
     2221 tgaccggctg tgttgatagt ctgtgcacct gataaaccta cgagtgtttt tgcagctttt
     2281 gcttcgtcga gaagtctgat gtcta
//
LOCUS       DAOSHR010000283         2301 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_45972_length_2301_cov_3.473731, whole genome shotgun sequence.
ACCESSION   DAOSHR010000283 DAOSHR010000000
VERSION     DAOSHR010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45972_length_2301_cov_3.473731"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="PLU28_10765"
     CDS             <1..248
                     /locus_tag="PLU28_10765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35583.1"
                     /translation="IVKFDSLIDLTGFWYRFISDYSTNLKSATSALPLIYGKIDITYK
                     EKIYACWFTGIEQIMFVVVGTDKPSIDDLRYKLSNYR"
     gene            250..1164
                     /locus_tag="PLU28_10770"
     CDS             250..1164
                     /locus_tag="PLU28_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208587.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HOG35584.1"
                     /translation="MKRYNKLSTYLKGLYGQRVQRISIDAGFSCPNRSGEKGNEGCFF
                     CDATGSGFASFGSGYSIQQQVSLMIEKYSNRAEKFILYFQSNTNTYAPVSKLKEIYDS
                     GLCDDRIVILDISTRPDCVKDEVLELVSSYKDRYGVFIEYGAESVNPNTLKRFNRRHT
                     YAEYIDAVIRSKKKGLDVVTHFIVDFPCDSYDDVIEMAKSSSALGISGVKLHSLYYLE
                     NTELGRMYKQKEISPVSFEEYIDRLILFLEYLDPAIVVHRLVSEPPSEGVMHGNWGMK
                     KLELFNYVEKELERRNTYQGKYYNYLNR"
     gene            1165..1499
                     /gene="rnpB"
                     /locus_tag="PLU28_10775"
     ncRNA           1165..1499
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLU28_10775"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(1572..2072)
                     /locus_tag="PLU28_10780"
     CDS             complement(1572..2072)
                     /locus_tag="PLU28_10780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35585.1"
                     /translation="MKKAVSIFMMLFLVLVFSSVAFADDASRNSGPAIRNTNNQANYQ
                     NLQQKQQLLKRYQNSQNNKNICPFMVPAPKNNIAPSRQMPLYRGMMLYKRMDNAKDLI
                     SKAKNLKSNALGVFSQIREYSNQMNEALNSNDIEKAKELSSKLEELLNKVNSKLTPDA
                     TSTSPQ"
BASE COUNT          702 a          392 c          507 g          700 t
ORIGIN      
        1 atattgttaa atttgacagt cttatagatc ttaccggatt ttggtatcgc tttatatctg
       61 attactccac taatcttaaa tcagcgactt ctgccctgcc gctgatttac ggaaagatag
      121 atataactta taaagaaaag atttatgcct gctggtttac cggaatagaa cagataatgt
      181 ttgttgtagt tgggacggac aaaccttcta tcgacgatct aagatataag ctgagcaatt
      241 acaggtaaga tgaaaaggta taataagctc agtacatatc tgaagggttt atacgggcag
      301 cgtgttcaga gaatatcaat tgatgcgggt ttttcttgtc caaacagaag cggtgaaaaa
      361 ggaaatgaag gctgtttttt ctgtgatgcc acgggaagcg gctttgcctc tttcggttca
      421 ggctactcaa tacagcagca ggtaagtctt atgattgaaa agtattcaaa ccgtgctgag
      481 aagttcatac tctactttca gtccaatacg aatacatatg ctcccgtttc aaaacttaaa
      541 gagatatatg acagcggact ttgcgatgac agaatagtaa ttcttgatat ttccacacgt
      601 ccggactgcg tcaaagatga ggtgcttgag cttgtatctt cgtataaaga caggtacggg
      661 gtatttatag agtatggggc tgagagtgta aatcctaata ctcttaaacg cttcaaccgc
      721 cgtcatacgt atgccgagta tattgatgcg gttatcagaa gcaagaaaaa aggtcttgat
      781 gtagtaacac attttatagt ggactttcca tgtgacagtt atgatgatgt gatagaaatg
      841 gccaagtcaa gttcggcact gggcattagc ggtgtgaagc tgcattctct ttactacctt
      901 gaaaatacag aacttggacg tatgtataaa caaaaagaaa tatctccggt aagctttgaa
      961 gagtatatag accgtcttat actatttttg gagtatcttg atccggctat agtcgtgcac
     1021 cgtctggttt cagaacctcc cagcgaagga gttatgcacg gaaattgggg aatgaaaaaa
     1081 ctggagcttt tcaactacgt ggaaaaagag ttggagagaa gaaatactta tcagggaaaa
     1141 tactataatt atttaaacag ataatcaggt tggagtgtct gaccgcaggc agagaaagtc
     1201 tgtcctgagg aaagtccgga ctcatagggc aggacgccag ttaacgactg ggagaagtga
     1261 ttctcggtaa gggccataga aacgtaaacc gctctgaaaa agagcaaggg tggaaaggtg
     1321 gggtaagagc ccaccagggt cagggtaacc tggccgcttg gtaacctccg tcttgagcaa
     1381 gatcaaaacg aaaggtcata ggatgcccgc caaattgagt tttactcata cctttcaggt
     1441 agatcgctta gatagatggt caggctagac agaatccggc ttacagctta cctgatactt
     1501 ttttaaaata aaacagccta aaaggctgtt ttatttttta cttattctta cttggggttt
     1561 gggtttcatt ctcattgggg gcttgttgag gttgcatcgg gagttaattt tgaatttacc
     1621 ttattcaaaa gttcttccag ttttgaagaa agttctttgg ctttttcaat atcattggaa
     1681 ttaagggctt cattcatctg gtttgagtac tctctgatct ggctgaaaac tccaagagca
     1741 ttactcttta agttctttgc ctttgaaatt aggtcttttg cattatccat tcttttataa
     1801 agcatcattc cacggtataa cggcatctgt ctggaaggag ctatgttatt tttcggggca
     1861 ggaaccataa acggacagat atttttattg ttctgagaat tctggtatct cttaagcaac
     1921 tgctgtttct gctgtaaatt ctgatagttt gcttgattat tggtgtttct gattgccgga
     1981 ccggaatttc ttgacgcatc atcggcaaat gctacagaag aaaaaacgag cacaagaaat
     2041 agcatcatga atatacttac tgcctttttc atactttcac catcctttta aattatattt
     2101 actcctttat tatataaaag aactgtagcg ggagaatatg agaatctggc aaaattgtga
     2161 gaaatggaaa agaactttct agtatgttaa aatatactta agtaaagtga ttttaaagga
     2221 ggacagtatg ccggatataa aaaccatcta tgtagtggac gacgaagaaa aaatattaga
     2281 actcataaaa tcttatcttt t
//
LOCUS       DAOSHR010000284         2300 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_46000_length_2300_cov_3.386637, whole genome shotgun sequence.
ACCESSION   DAOSHR010000284 DAOSHR010000000
VERSION     DAOSHR010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46000_length_2300_cov_3.386637"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1685)
                     /locus_tag="PLU28_10785"
     CDS             complement(<1..1685)
                     /locus_tag="PLU28_10785"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG35586.1"
                     /translation="MTGRFFIRSLLIVLILFVIGIIFIVLFNQNLAEQEVLKINRAGF
                     SLASKIEDLVNRPLSAAYALAAIIKTYGGFSSQQEFESIAQKITELYDGVSFLAAAPN
                     AVTSFIYPLQGNEKAIGHDLLNDPQRRDDVLRAIQSRSLTLSGPYELVQGGVSILGRL
                     PVFRDGTESDFWGLSVVSLDLSKLLYDLELDSMLDSGFLYEITRKDSPSDQETIIITT
                     IGKDFNKKKYVFSLNLPNSLWYLHIIPKKNGTYNMLVWEVLVLIIVLLIGFVIAYRLI
                     KQNYDIKAANMELHMQIKEKERIELELIAAKKNAETANNAKSLFLANMSHEIKTPLNG
                     IMGFISLLKKTSLDTEQQELVKQAEISGHSLTGLISDILDISKIEADKVELHNEPYEI
                     RKLIEEVGTVFLPLVKEKNIKLSIKISEDTPKILELDTVRLKQILINLIGNAVKFTEQ
                     GEVSLSVKPLQSKEDRCKLLFTVKDSGIGIAEKDFDKLFKSFSQVDSSTSKKYGGTGL
                     GLSISKKLIELMGGEITFESTEGYGSKFDFFILVQTFAGQIDPKNNKSAITNKT"
     gene            complement(1682..>2300)
                     /locus_tag="PLU28_10790"
     CDS             complement(1682..>2300)
                     /locus_tag="PLU28_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35587.1"
                     /translation="FIIKRRNSLYNCGFLFDRSGRMVLEQMKTHVTPSEEEIGIKCCA
                     SLETFLINDVRFAFPICMDASYFEVFRILMDMNTDVAVIPIANNENYDIYRAARGIWA
                     RVQETPMYGIKSSLNGWFLGQNFTGKAGIFAPVNLTASQTGVIAISSDPVGDFLVTSL
                     IDPSSIRLYSKSNEYSVHHNFDFETQNFIAYKKYNPNTIKKKRKP"
BASE COUNT          670 a          446 c          401 g          783 t
ORIGIN      
        1 gttttgttgg taatcgcaga tttattgttt ttagggtcta tctgtccggc aaaagtttgt
       61 actaatataa aaaaatcaaa tttggagcca tatccttctg tgctctcaaa ggtgatttct
      121 ccacccatga gttcaatcaa ttttttggat atagaaagtc ccagtcctgt gccaccatat
      181 tttttagatg tagaagagtc aacctgactg aagcttttaa aaagtttatc aaaatccttt
      241 tctgcaattc ctatcccgct gtcttttaca gtaaatagaa gcttgcacct atcttctttg
      301 ctttgaagag gtttgaccga taatgaaact tctccctgtt cggtaaattt gacggcattt
      361 ccgataagat tgatgagtat ttgtttgagt cttactgtgt ccagttcaag aattttagga
      421 gtatcttcgc ttatttttat ggataacttt atattttttt ctttcaccaa aggaagaaat
      481 acagtaccta cttcctctat caactttctt atctcatatg gttcgttgtg cagttctact
      541 ttatcagctt caatttttga aatatcaagt atatcagata tcagccctgt caatgaatga
      601 ccggaaatct ccgcctgttt tactagctct tgctgttctg tatcaagaga agttttttta
      661 agaaggctta taaatcccat tataccgtta agcggtgttt ttatctcgtg actcatgttg
      721 gccaaaaaaa gacttttggc attgtttgcc gtctctgcat ttttctttgc cgcaataagc
      781 tcaagttcaa tcctctcttt ttctttaatc tgcatatgaa gctccatatt tgccgctttt
      841 atatcatagt tctgctttat aagtctgtag gctattacaa agcctatcaa cagaacgatt
      901 attagaacta aaacttccca tacgagcata ttgtaagttc catttttttt agggataata
      961 tgaaggtacc acaaactgtt tggaagatta agactgaaaa catacttctt tttattgaag
     1021 tcttttccta tagttgttat tatgatcgtt tcctgatcgg aaggagagtc ttttctggtt
     1081 atttcataaa gaaatccgct gtctagcatg gaatcaagtt caagatcata aagaagcttg
     1141 cttaaatcca acgatactac ggacagtccc cagaaatccg actctgtacc atctctgaat
     1201 acaggaagtc ttcctaaaat tgaaactccc ccttgtacaa gctcataagg acctgaaagt
     1261 gtaagcgacc tgctttgaat agctcttaat acatcatctc ttctttgagg gtcgttgagc
     1321 agatcatgtc ctatagcctt ttcatttcct tgtaggggat atataaaaga tgtcactgca
     1381 ttaggtgcgg cagcgagaaa agatactccg tcatatagct ctgttatttt ttgtgcaatg
     1441 gattcaaact cttgctgaga actgaacccc ccgtacgttt ttatgatcgc tgccaaagca
     1501 taagcagccg aaagtggtct gttcacaagg tcttcaattt ttgaagcaag agaaaatcct
     1561 gccctgttta tcttcagaac ttcttgttcg gctaggtttt gattgaaaag aactatgaaa
     1621 atgattccta taacaaataa tattaataca atcagtaaag atctgataaa gaatcttcct
     1681 gtcatggttt tcttttcttt tttatagtat tgggattata ttttttataa gcaataaaat
     1741 tttgggtctc aaaatcaaag ttatgatgca cagaatattc gttgcttttt gaataaagcc
     1801 gtatagaaga aggatcgata agagaagtaa ccaaaaaatc acctaccggg tcgctactta
     1861 tggcaatcac accagtttga gaggccgtta aattaacagg tgcaaatatt ccggcttttc
     1921 cggtgaagtt ctgtcccaaa aaccaaccgt tcaagcttga ttttatacca tacataggag
     1981 tttcctgaac ccttgcccaa ataccgcgtg ccgctctgta tatgtcgtaa ttttcgttat
     2041 tggctatcgg aatgacagca acatcggtat tcatatccat aagaatacga aaaacctcaa
     2101 aatatgatgc atccatacat atcggaaatg caaagcgtac atcgtttata agaaatgttt
     2161 caagagaggc acagcatttt atgccaatct cttcctcgga aggagttaca tgtgttttca
     2221 tctgctcaag aaccattctg ccggatctgt caaataaaaa tccgcagtta tacagactgt
     2281 tcctgcgttt tattatgaag
//
LOCUS       DAOSHR010000285         2268 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_46905_length_2268_cov_7.971532, whole genome shotgun sequence.
ACCESSION   DAOSHR010000285 DAOSHR010000000
VERSION     DAOSHR010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46905_length_2268_cov_7.971532"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(144..1046)
                     /locus_tag="PLU28_10795"
     CDS             complement(144..1046)
                     /locus_tag="PLU28_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35588.1"
                     /translation="MIASNDILWQEPKVQSIEFRGSFFEKKILRCEEKFKNKDFIGYT
                     LSNKTLNSQEDSLTQTTKVFYPLIDAMSKVNSNMLPELMDVFVCESDNAKKLVLVMEY
                     IKGYPLKDAIDKYQTYKKKQADSYTINISNFSKLLKKLTYFSEDLLNEGYSYLGFFPS
                     HLILQKSDVLRITGLRFIMKTVKGIVTDSRLKSLENEIKYSFMPMELYTKLYEESKGP
                     IDALAVLAYQIGILILWVYHGQNLGKYRISNNQIELSDEIFEIIPYKEREKVSDAVWA
                     LLDPDPDIRINTLQEIRDCLESLE"
     gene            complement(1043..2113)
                     /locus_tag="PLU28_10800"
     CDS             complement(1043..2113)
                     /locus_tag="PLU28_10800"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOG35589.1"
                     /translation="MKLLGYLKKEVTTNVYRFIDPSNPERFVLSPKVVKDRYEITDLL
                     SNASGFGLVFIAKDTKLLNKKVLVKAINYKKAFNKNSLDAKEKILLRRKNLKLESQIL
                     LKLNNLNVASVPLFIDYIEGYSPAIQWPDGNVGVGTPAFNELAMNEPYFFLQYIKGQT
                     LSEKAKNTDKDSLSWQIEVLRVAQRILNIFDVIHSGDEKVESYIYQDLKPDNLIASPN
                     GTYTLIDFGGVALNMPGKNSTYNKGIGTPGYMAPEIADPAIMKIDRRADIYSLGVLIW
                     SLLMKEELINYIDESGIIKKVDLSSIEDYAYEPVCRIIEKAVQKDPNKRYKDSYQMYG
                     KISDALKKLNKINSCFREEEII"
BASE COUNT          699 a          431 c          333 g          805 t
ORIGIN      
        1 taccatcatc aataacagta taagttcctc cgcattcgca gaactcttct tcataaccca
       61 aatcaccagg aaacaaaact tttgagcata ttgcacatac tttcttcaat tcgatcttcc
      121 tcctttttaa aagtaaacgt tatttattca agtgactcta agcaatctct tatttcttgc
      181 agagtgttta ttctgatatc aggatcagga tcaagcaatg cccagaccgc atctgatact
      241 ttttctcttt ctttataggg aataatctca aatatctcat cagacagttc aatctggttg
      301 ttggaaatac gatacttccc aagattttgt ccatgatata cccacagaat cagaatacct
      361 atttgatatg ccagaacagc aagtgcatct atgggacctt tactttcttc atacaatttg
      421 gtgtagagct ccatcggcat aaaactgtac ttaatctcat tctccaaact ctttagcctg
      481 gaatctgtta ctatgccttt tactgtcttc atgataaatc ttaatccggt tattctcagc
      541 acatcacttt tttgaagtat taaatgtgag ggaaagaatc ctaagtaaga gtatccttca
      601 ttcaaaagat cttcagaaaa gtatgtaagc tttttgagga gttttgaaaa gtttgaaata
      661 ttaatagtat aagagtctgc ttgttttttt ttatatgtct gatacttatc aatagcatct
      721 tttaaaggat agccttttat atactccatc actagtacaa gtttttttgc attgtctgat
      781 tcacagacaa acacatccat caactccggc agcatgttcg agtttacttt gctcatggcg
      841 tctattaaag gatagaatac ttttgttgtc tgcgtcaaag agtcttcttg actgttaagc
      901 gttttgtttg aaagtgtata tcctataaaa tctttatttt tgaatttttc ttcacaccta
      961 agaatttttt tctcaaaaaa ggagcctcta aattctatgc tttgaacttt cggttcttgc
     1021 cataaaatat cgttagaagc tatcatataa tctcctcttc tctaaaacaa ctgttaattt
     1081 tattaagctt tttcaaagca tccgatatct tcccatacat ctgatacgaa tctttgtatc
     1141 ttttgttggg atctttttga acagcttttt caattattcg acataccggt tcataagcat
     1201 agtcttcaat gctgctaaga tcaacttttt ttataattcc gctttcatca atataattga
     1261 taagctcttc tttcattaaa agactccata taagtactcc aagagagtag atatcagctc
     1321 ttctatcaat cttcattata gcaggatctg caatttccgg agccatatac ccaggagtgc
     1381 ctatcccttt attgtatgtg gaatttttgc caggcatatt aagcgcaaca cctccaaaat
     1441 ctataagagt atacgtaccg ttgggactgg caataagatt atccggctta agatcctgat
     1501 agatatagct ctcaaccttt tcatctccgg aatgaattac atcaaaaata ttcaaaattc
     1561 tttgtgcgac acgtaaaact tcaatctgcc atgataaaga gtctttatct gtatttttag
     1621 ctttttcgga aagtgtctga ccttttatat attgaagaaa aaagtaaggc tcattcattg
     1681 ctaactcatt aaaggcaggt gttcccacac cgacattacc atcaggccat tgaattgcgg
     1741 gagagtatcc ttcaatataa tctataaaca gaggaacaga agcaacgttt aaattattta
     1801 gttttaaaag tatctgactt tcaagcttta agttttttct tcttagaaga attttttctt
     1861 ttgcatctag gctattttta ttgaaagctt ttttatagtt gattgctttt acaagaactt
     1921 tcttatttaa aagctttgta tctttagcaa taaaaacaag tccaaaacca gaagcattgg
     1981 aaagcagatc cgttatttca tatctgtctt ttacgacttt ggggcttaat acaaagcgtt
     2041 ccggattgga aggatctata aatctatata cattggtagt tacttctttt tttagatatc
     2101 ccagaagctt caccccctta ctacctccaa ataattattc gacatttgta aaattaaata
     2161 aaaaaatacc gcagaaaata ttctgcggta ttttattttt gacaattata aataaacaaa
     2221 accccagtct aaaaaagact ggggaaaagt gaaggggcga tacctact
//
LOCUS       DAOSHR010000286         2265 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_46993_length_2265_cov_5.430769, whole genome shotgun sequence.
ACCESSION   DAOSHR010000286 DAOSHR010000000
VERSION     DAOSHR010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46993_length_2265_cov_5.430769"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..650
                     /locus_tag="PLU28_10805"
     CDS             <1..650
                     /locus_tag="PLU28_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35590.1"
                     /translation="NCKGLLEDIYAKIYINKIESIKPVFSYPFYVEEIEETPIATLDF
                     YESSKLEAIYNTFQRKLKDDLDEYVKSATEKSCLVGVFKIDKFYLDTNNIEDIWLKYN
                     EAYLSGTLKNEFIKYTTKSSKYSLKINEAYITKYDLSSILNKDKNQNGFISLFSDSFI
                     RDYTVEENNNVYTSIFTINNIMIDNKKNFTLYINGKLEIGEIDIPFEINQTINLN"
     gene            793..971
                     /locus_tag="PLU28_10810"
                     /pseudo
     CDS             793..971
                     /locus_tag="PLU28_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611142.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1099..2064
                     /locus_tag="PLU28_10815"
     CDS             1099..2064
                     /locus_tag="PLU28_10815"
                     /inference="COORDINATES: protein motif:HMM:NF013798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HOG35591.1"
                     /translation="MPFTIVCQDITKLKVDVIVNASNTYLKMGGGVCGAIFKAAGAEK
                     LQAVCDKLAPIEIGNAVITPGFDLPAKCIIHTVGPIYQHWSKKESEKYLCAAYTNSLK
                     LAFEKKYESIAFPLISSGIYGYPKDEALQVALSCIKNFLFSHDIDVTLVVFDKSSFSI
                     SNKLLSAVKSYVDEHYVDVHEKSIKPGKEPSIEDSSASANQFMGLIDSLDESFSKTIM
                     RLISSKGRTEVEVYKRANIDRKLFSKLRNNESYMPSKRTAIALAVALELSLDETSDLL
                     KRAGYTLSNSFKFDVIVQYFIVNGIFDIFEINNVLFNYDLPLLGY"
BASE COUNT          793 a          327 c          410 g          735 t
ORIGIN      
        1 ataattgcaa aggattgctg gaagatattt atgcaaaaat ttatataaac aaaatcgaaa
       61 gtattaaacc agtattttct tatccttttt acgtagaaga aatagaggaa acaccgatag
      121 caacccttga tttttatgaa tcatcaaaac ttgaggctat ttataatact tttcagagga
      181 aattaaaaga tgatttagat gaatatgtta agtcagcaac tgaaaaatca tgtcttgttg
      241 gtgtatttaa aattgataag ttttatttgg atactaataa cattgaagat atttggttaa
      301 aatataatga agcatattta tccggaactt taaaaaatga atttatcaaa tacacaacaa
      361 aatcgtctaa atattcatta aaaataaatg aagcatatat aactaaatat gatttaagct
      421 ctatactaaa taaagataaa aaccaaaatg gctttattag tcttttctca gatagtttta
      481 ttcgagacta tacagtagaa gaaaataata atgtatatac ttcaatattt acaataaata
      541 atataatgat tgataataag aaaaacttta ctttatacat taatggaaaa ttggaaatag
      601 gagaaataga tattccattt gaaataaacc agactatcaa tttaaattaa aaaagctccc
      661 gtaaaaaaag tgaagtgaac ccctttagtt agagagaatc tgacgaaagg ggttttgtta
      721 tgtcaagaaa aagaaagtat tcggatgagt ttagactaat gttagttgat gagtatctct
      781 ccggaaaaaa cggagaactt ctttggctta cttaaatctg agctacttta tcttcgcgag
      841 tttgaaagtt ttgaagagtt ccgcgaggaa cttgaaaata catatgctac cacaatcatc
      901 ataggatcaa gggcaagtta aaaagactga gacctgttca atacagaact cagtccttgt
      961 taatagctta atgtaccacc aaatgtgtct gatttttggg ttcagttcaa aaaaaccatc
     1021 tatcaacaaa tgatggtatt attttatata aaagcttaag tgatgttttt tatgcttatg
     1081 ccaggaggga gttttattat gccttttacc atcgtatgtc aggatatcac aaagcttaag
     1141 gtcgatgtca ttgttaatgc ttctaatacc tatcttaaaa tgggcggcgg agtttgtggt
     1201 gcaattttta aggcggctgg tgcagaaaaa cttcaagctg tatgtgacaa acttgctcca
     1261 attgagatcg gaaatgccgt catcactccg ggctttgatc ttccggcaaa gtgtatcatt
     1321 catactgttg gaccaattta tcagcactgg agcaaaaagg aaagtgaaaa gtacctgtgc
     1381 gcagcttaca caaactctct taaactggct tttgaaaaga agtatgagag tattgccttt
     1441 cccttgattt caagtggtat ctatggttat ccgaaggatg aggctctaca ggtggcatta
     1501 tcttgtatta agaattttct tttcagtcac gatattgatg ttacgcttgt tgtgtttgat
     1561 aaatcatcct tttctataag caataagctg cttagcgcag ttaaaagtta cgttgatgag
     1621 cactatgttg atgttcatga aaaatccata aaaccgggta aagaaccttc tattgaagat
     1681 agttctgctt ctgctaatca atttatggga ctgattgaca gtcttgatga atctttttcc
     1741 aagactatca tgcgtttaat ttcttctaag ggcagaacag aggtggaagt ctataaacgt
     1801 gcaaacattg acagaaaatt attttcaaaa cttcggaaca atgaaagcta tatgcccagc
     1861 aagcggacag caattgcttt agctgtggca ttagaactgt ctcttgatga aacaagtgat
     1921 ctgctgaaac gcgcaggata taccctatct aattctttca aatttgatgt aattgtacag
     1981 tattttattg taaacggtat ttttgatatt tttgagatta acaacgtact tttcaattat
     2041 gatctgcctc tattaggtta ttgagacttg tacctaggtt tttgtcgctt gatatgcgac
     2101 cttttagatt ttgagaacct gtataatccg gataaaccaa gtattaagaa ggtatcttca
     2161 gaatgaaaaa aagcttttga tgcaatacgt aaagatgtga agaacagagg ttttcaaaag
     2221 taacgatttt atacgataaa acaggagagt tgatatgcat aaaaa
//
LOCUS       DAOSHR010000287         2253 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_47402_length_2253_cov_3.597816, whole genome shotgun sequence.
ACCESSION   DAOSHR010000287 DAOSHR010000000
VERSION     DAOSHR010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47402_length_2253_cov_3.597816"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="PLU28_10820"
     CDS             complement(<1..258)
                     /locus_tag="PLU28_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35592.1"
                     /translation="MINTNSKILKRIYKDRYYYYLLIPATAYFIIFHYIPIYGVKLAF
                     EKYRIFGGNQWVGLAYFEELFTSPAFYKALMNTIIISGLKLL"
     gene            complement(265..1668)
                     /gene="citF"
                     /locus_tag="PLU28_10825"
     CDS             complement(265..1668)
                     /gene="citF"
                     /locus_tag="PLU28_10825"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HOG35593.1"
                     /translation="MNKVFNSFEELFREIPVNDGMTISFHHHLREGDAVMFQVLKSIQ
                     KTGVKGITLACTSLMNTHDFIADMISEGTVSRIQTSGLKGKLAAMALNGGMQKPVIFR
                     SHGSRARAVSDGELKVDIAFIAASAADMFGNANAIDGPNAFGSLGYGVWEAQNAKKTV
                     IVTDYLSRGILRDISISQSYVDAVMIVPAIGNTAGVKEGSLKERSNPRDSVIAADVAK
                     TIINCGIELDQSGIQMGSGSVSLQVGRYLSGYMKERDKKFKFGLGGITQDLIDMLNNG
                     TFESLLDVQSFDPKACNSIRNNKNHHEIDVNTYANPLNRSSAVNFLDIAVLSATEIDT
                     DWNVNVLTGSNGSIIGAIGGHQDVAAASKITIIAAPLFRNRIGVIRKKVTTVVTPGEN
                     IDILVTDFGIAVKESRQDIIKNLTDNGIKVLKIEDLYKKCISFTSEPCTVRFTDKVAA
                     IVQTPDGKVQDYIYKKV"
     gene            complement(1661..>2253)
                     /locus_tag="PLU28_10830"
     CDS             complement(1661..>2253)
                     /locus_tag="PLU28_10830"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HOG35594.1"
                     /translation="PKVQKAQEIVYIDQSITEIENELSLPGGIIKFMVSIESALGVIN
                     IKEICQASTRLIGVAFGAEDFTYDMGVKRTKEGNELAFARSTLALTARAYGKIAIDCV
                     FSDTKDPEGLRKDCEIARSFGMTGKSVIHPGQIEVVNKSFSPSDQEIGQARRVIELYK
                     KLGCPGVFSFEGNMVDLPVLKKYQRIYELYGRDKVE"
BASE COUNT          606 a          520 c          384 g          743 t
ORIGIN      
        1 aagcagtttt aacccggaga ttataatagt attcataagc gctttataaa acgccgggga
       61 tgtaaacagc tcttcaaagt acgcaagtcc tacccactgg ttcccaccaa atatcctata
      121 cttttcaaaa gcaagcttga cgccatatat gggaatgtag tggaatatta taaagtatgc
      181 ggtcgccggg ataagaaggt agtagtagta cctgtcctta taaatacgct tgagaatttt
      241 actgtttgta ttgatcatcg catatcacac cttcttatat atataatcct gcacctttcc
      301 atccggagtc tgtactattg ctgcaacttt atccgtaaac cggactgtac atggttccga
      361 agtaaaagaa atacattttt tatatagatc ttctatcttg agaaccttta ttccgttgtc
      421 tgtaaggttt tttataatgt cctgcctgct ttcttttact gcgattccaa aatctgtgac
      481 aaggatatct atattttctc ccggagtaac cactgtggtt actttttttc tgataactcc
      541 gatcctgttt ctgaaaagag gtgctgcaat aatagttatt ttacttgccg ctgccacatc
      601 ctgatggcct cctatggcgc ctattatact tccgttagat cctgtcagta cattaacgtt
      661 ccagtctgta tcaatctctg tggcactgag gactgcaata tcaagaaagt taaccgctga
      721 acttctgttg agtgggttag catatgtatt tacatcaatt tcgtgatgat ttttattgtt
      781 tcttatagag ttacaggctt tcggatcaaa gctctgtaca tccagcaaag actcaaaagt
      841 tccattattg agcatatcta taagatcctg agttatacct cccaacccaa atttgaattt
      901 tttatctctt tccttcatat acccgctaag atatctgcca acctgtaagg agaccgaacc
      961 actgcccatt tgaattcctg attgatcaag ctctataccg cagtttataa ttgtttttgc
     1021 aacgtcagcg gctataacag aatctctggg attgcttctt tccttgagtg acccttcctt
     1081 tactcctgct gtatttccta tggcaggaac aatcatcacg gcatcaacat aagattgact
     1141 tatacttata tctcttagaa tcccccggga aagataatcg gttactataa ctgtcttttt
     1201 tgcattctga gcctcccata ctccatatcc caacgatccg aaagcatttg gtccgtcaat
     1261 tgcattggca tttccaaaca tatccgcagc ggaggcagca ataaatgcaa tgtcaacctt
     1321 cagttcccca tcgcttacag cacgggccct ggaaccatga gacctgaata ttacaggttt
     1381 ctgcatgcct ccgtttaaag ccatagcagc aagctttcct tttaaacctg atgtctgaat
     1441 tctgctgact gttccttcac ttatcatatc agcaatgaaa tcgtgtgtat tcataagcga
     1501 agtacatgca agagtaattc ctttcactcc ggttttctgt atactcttaa gcacctgaaa
     1561 cataactgca tctccttcac ggagatgatg gtggaaagag atcgtcatac catcattaac
     1621 cggtatttcc ctgaaaagtt cttcaaacga attaaaaact ttattcaact ttatcacgtc
     1681 cgtaaagttc atatattcgc tgatattttt tcagtaccgg aaggtcaacc atgtttcctt
     1741 caaaactgaa tactccggga catccaagct ttttatataa ttcaattact ctgcgtgcct
     1801 gcccaatctc ctgatcagaa ggcgagaatg atttatttac aacttcaatc tggccgggat
     1861 gaatcactga ttttccggtc attccaaatg accttgcaat ttcacagtct tttcttaaac
     1921 cttcagggtc tttggtatcg gaaaaaacgc aatcaatggc tatcttcccg tatgccctag
     1981 ctgtcaatgc cagtgttgac cgggcaaatg ccagttcatt tccttctttg gttcgtttaa
     2041 ctcccatatc gtaagtaaag tcttcggctc cgaacgctac tcctataagt cttgttgaag
     2101 cctggcagat ttcttttata tttatcacac ccaaagcaga ctctatgctt accatgaact
     2161 ttattatgcc gccaggcaga ctcagttcgt tttctatttc tgtaatgctt tgatctatat
     2221 atactatctc ttgggctttc tgaaccttag gaa
//
LOCUS       DAOSHR010000288         2231 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_48073_length_2231_cov_6.395680, whole genome shotgun sequence.
ACCESSION   DAOSHR010000288 DAOSHR010000000
VERSION     DAOSHR010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48073_length_2231_cov_6.395680"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            220..>2231
                     /locus_tag="PLU28_10835"
     CDS             220..>2231
                     /locus_tag="PLU28_10835"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOG35595.1"
                     /translation="MYKNLEWAVQEVKSYFPLADDFEGFMGVFINRYLLKNSIKDAGI
                     LEMTQMYFPYSESTYQKHCQDIGNIFDRLDCWEKDDMLCWVYQYYGNDTRNMSKSKLR
                     NKAQKKELIHAKIYTPLWIVDYLTGGAFAYFGLNEMKATEIKKIRILDPACGSGNFLI
                     RIYDYLMQLYTDKGVEKNQACKLILEKNIFGCDIDSYGIKIAKIILACKAYKDGADKP
                     VKINLSCFDEKNINLKTAAEYRTLGSLYPLGDSKFLKEENFDIILSNPPYTDSSDYSA
                     ELKAKIDMYYKNYRKNLYACFIIRSHSLLKKNGICAMITPQTFMFISSFKQTRSFILD
                     NMNIKEFVHFGLGGVFDNALVDTCAFILKKEKHLSSVARYIKLDGTAACDKKATLHRV
                     LEGKFPELCYFSDQNDFESIPGKPFVYWISQEFRDIFSNPTLMEFADVRQGIATGNNK
                     KFLRYFWEVEKNSIRFDTSDNSKKWVPYAKGGPYNKWYGNLWWVIAFDSQSTEILQNT
                     GNNLPNKEYYFRSGITYSMTTSSGSTFRYLPQGFLFDCKGSSVFCHDDSHTFRFLALL
                     NSKLAFFIYGFIAGSVDLEVGDLRNLPVHKDLLEENSLSLRLDRASRLLTAIRMKKEA
                     YKATDMNYKLSEIHQVIGLGKGFDIQVLENTLERRDFLDLFISVLEG"
BASE COUNT          730 a          377 c          452 g          672 t
ORIGIN      
        1 ccaaaaactg gctgcctgtc tgcatgataa agtatatgaa cctgcatgaa gcctttatat
       61 agtttatcca gaattttttt ggtaatgata tcacctcccg atatcagttt tcgatatgta
      121 ttatacacat tatattttgc ttttatgtaa acaccttttt cttgtagata aagtacttat
      181 agggtataat ttaaaagatg ttttttttga ggtgagaaaa tgtataaaaa tcttgaatgg
      241 gcagtacagg aggtaaaatc atactttccc cttgctgatg attttgaggg atttatgggg
      301 gtatttataa accgctatct gctcaaaaat agtataaaag atgccggaat acttgagatg
      361 acacagatgt attttccata ttctgagagt acataccaaa aacactgtca ggatatcggc
      421 aacatattcg accggctgga ctgttgggaa aaagacgata tgctttgttg ggtataccag
      481 tattatggca acgacacaag aaatatgtcc aaaagcaagt taagaaataa ggcacagaaa
      541 aaagagctta tccatgcaaa gatatacacg ccgttatgga ttgtggatta tcttaccggc
      601 ggagcgtttg cttattttgg tttaaatgaa atgaaagcaa cagaaatcaa aaaaatccgc
      661 atacttgacc cggcatgtgg aagtgggaat tttctgattc gtatatatga ctatcttatg
      721 cagttgtata ctgataaggg agttgaaaaa aatcaggcct gtaaattaat acttgaaaaa
      781 aatatcttcg ggtgcgatat tgatagttac ggtataaaaa tagcaaagat aatactagcc
      841 tgtaaagcat ataaagatgg tgctgataag cctgtaaaga ttaacctctc gtgctttgac
      901 gaaaaaaaca taaatctgaa gacggcagcg gagtacagaa ctctgggaag tctttatcct
      961 ttgggtgact ccaagtttct caaagaggaa aactttgata ttatattaag caatcctcca
     1021 tacacggatt cctctgatta ttctgccgaa ctaaaagcta aaattgacat gtactataaa
     1081 aattacagaa agaatttgta tgcctgtttt ataatacgca gccattctct gctcaaaaaa
     1141 aacggtatat gtgcaatgat tactcctcag actttcatgt ttataagcag ttttaaacag
     1201 acgcgttcgt tcattcttga caacatgaat ataaaagagt tcgtccattt cgggctcggt
     1261 ggggtgtttg ataatgctct ggttgacacc tgcgccttta tcctgaaaaa agaaaaacac
     1321 ctgtcatctg tagcaagata tataaaatta gacggaactg ctgcctgcga taaaaaagct
     1381 acgcttcaca gagtacttga agggaaattc ccagagttat gttacttttc agatcagaat
     1441 gattttgaaa gtattcccgg aaaacctttt gtttattgga tttcccagga gttcagagat
     1501 attttctcta atcccacgct aatggagttt gcagatgtcc gccagggaat agcaacaggt
     1561 aataataaga aatttttgag atacttctgg gaggtcgaaa agaacagtat aaggtttgat
     1621 acgtctgata attcaaaaaa atgggtgcct tatgccaaag gcgggcctta caataaatgg
     1681 tacggaaatc tctggtgggt aatcgctttt gacagtcaga gcaccgaaat tttacagaat
     1741 acgggaaaca atcttccgaa taaagagtat tatttccgaa gtggaataac ctattcaatg
     1801 acaacatcat ctggttcaac tttcagatac cttccgcagg gttttctctt tgattgtaag
     1861 ggaagctctg ttttttgcca tgatgactct cataccttca gatttctggc tcttctgaac
     1921 agcaaactgg cattttttat ttatgggttt atagccggaa gtgtggatct ggaagttggc
     1981 gatctaagaa atcttccagt tcataaggat ctcttagagg aaaacagtct gtctttgaga
     2041 ttggacagag catctagact cctcacagcc ataagaatga aaaaagaagc atacaaagcc
     2101 acagatatga actataagct ttctgagata caccaggtca taggcttagg caagggcttt
     2161 gatatacagg ttcttgagaa tacacttgaa agaagagatt ttctagacct ttttatctct
     2221 gttcttgaag g
//
LOCUS       DAOSHR010000289         2219 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_48454_length_2219_cov_6.249538, whole genome shotgun sequence.
ACCESSION   DAOSHR010000289 DAOSHR010000000
VERSION     DAOSHR010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48454_length_2219_cov_6.249538"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2219)
                     /locus_tag="PLU28_10840"
     CDS             complement(<1..>2219)
                     /locus_tag="PLU28_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35596.1"
                     /translation="FKDIWQIKPREPYFVDRKNEKELINQIVLENLSYKKLLVYGPQR
                     SGKTSLLSVIKNEMISSDDYSVIEAHSKQDIYSGLLQIISAIENIDYTVLNDISNLNI
                     KIFDLLNILNTHKKFKIAVFIDDYHDYENVFSDLLSLMENIDFQFPLILIVCTNSYEL
                     VLKKQYSNFIKIELRPFQIEDTVTVLTSMLSSSFAKKNQDFINFIQNVSNGLPGNIKS
                     LIHDMEESGTLFFKNGDWQVDFDKIELNDYYSFILEKISKIEPVFQRELSLLSVLGNN
                     FQKQDIKELSIILGKDFDIQGLLKTEILQIEAEHYRFFDSLYWQAFYDRLDKTERNKI
                     HVLISKNINEFSKKIWHLKKAKQDALIALNFTQKMKRCIDTWSDIEFIDFGYSELQNM
                     GLTNDCATVIYVYTSIISENYIEISELIDSLKKKRWMRFYYLLAKSFFSPRQTIEEVD
                     SILNNTGNSDFQILYLNYVKLLACSFLENLGDLNELEQIFETVERIYSKHRNLRKFTE
                     IYLDSCIAFDSIVKAFDRIRSINILESALLLAKQQNLKKFEIAINTALAHNYINESPV
                     LFENMLKEITILSNKSNDFSRLATVYATHSFHSLYSGNVEETLKGIQESQKYSRQSKN
                     LKADATCRYIKALLYFYSGDFNNMNIELARDLAESLKMPDYLQKRYKLHYLIVKVMYA
                     ISIDARQSVLSYRKELDEFRMSAHDIIAYTDLYLLDNPEDIKRNFLSLFDSGTYQEET
                     "
BASE COUNT          680 a          365 c          398 g          776 t
ORIGIN      
        1 gtttcctcct gataagttcc actgtcaaaa agagagagaa agtttctttt tatatcctca
       61 ggattatcaa gcagatagag atcagtatag gctattatat cgtgtgcaga cattctgaac
      121 tcatcaagct ctttgcggta tgacagaacg gactgtctgg catcaatcga tatggcgtac
      181 atgaccttga ctataagata atgaagcttg tacctttttt gcagataatc aggcattttg
      241 aggctttccg caagatcacg cgccagttct atgttcatgt tgttaaaatc accactgtaa
      301 aagtataaaa gagcctttat ataacggcag gtagcatcag cctttaagtt tttggactgt
      361 ctggagtact tctgtgattc ctgtatgcct ttaagcgttt cttctacatt tccagagtaa
      421 agactgtgga atgagtgggt tgcatataca gtggcaagtc ttgaaaaatc gttggattta
      481 tttgaaagga tagttatctc tttaagcatg ttttcaaaaa gcacagggct ttcattgata
      541 taattatgag ctaaagcagt attaatggca atctcaaatt ttttaagatt ctgttgcttt
      601 gccagcaaaa gagcgctttc aagaatattt atagatctta ttctatcaaa agcctttact
      661 attgagtcaa atgcaataca tgaatcaagg tatatttctg taaattttct taagttgcgg
      721 tgtttggagt atatacgttc tactgtttca aagatctgtt cgagttcgtt aagatcgcca
      781 agattttcca ggaacgaaca tgcaagtaac ttaacataat tcagatataa gatctgaaag
      841 tcagagtttc ctgtattatt tagtattgaa tcaacctctt ctatggtctg ccttggagaa
      901 aaaaagcttt tagcaagaag atagtaaaac cgcatccatc tctttttttt cagagaatca
      961 atgagttctg aaatctcaat ataattttcg gaaatgattg aggtatagac atatatgact
     1021 gtagcacaat catttgtaag gcccatattc tgaagttcgc tgtatccaaa atcaatgaac
     1081 tcaatatcgc tccatgtatc aatgcagcgt ttcatttttt gtgtaaagtt tagagcgata
     1141 agagcatcct gcttggcttt ttttaggtgc cagatttttt tgctgaactc atttatattt
     1201 tttgaaatca gtacatgaat tttatttctt tcagtcttgt caagtctgtc ataaaaagcc
     1261 tgccaataga gtgaatcaaa gaaccggtaa tgttctgctt ctatttgcag tatttcggtt
     1321 tttaacaagc cctgtatatc aaaatctttt cccagaataa tagaaagttc ttttatatcc
     1381 tgtttctgga agttgtttcc taaaaccgaa agaagagaaa gctccctttg gaaaaccggt
     1441 tcgatttttg aaattttttc caggataaaa gagtaatagt cattaagctc aattttatca
     1501 aaatcaacct gccaatcgcc gtttttgaaa aaaagtgttc ctgattcctc catatcgtgt
     1561 ataaggcttt ttatatttcc agggagacca ttggaaacat tctggataaa atttatgaag
     1621 tcctggttct ttttagcaaa tgaagaagat agcattgaag tgagtacagt taccgtatct
     1681 tcaatctgaa aaggccgtag ctcgatcttt ataaaattac tgtactgctt ttttaagacc
     1741 agttcataac tgtttgtaca tacaatcaat attaaaggaa actgaaaatc tatgttttcc
     1801 atgagtgaaa gaagatctga aaaaacattt tcatagtcat ggtagtcatc tataaaaact
     1861 gctatcttaa attttttatg tgtattaagg atgtttagaa ggtcgaaaat ttttatgttg
     1921 aggtttgata tatcgttgag cacagtgtaa tctatatttt ctatggcact tattatctga
     1981 agaagaccgg aataaatatc ctgtttgctg tgggcctcta taacagagta atcatctgag
     2041 gagatcatct cgttttttat tacagaaagc aaagacgttt tccctgatct ttgtggtccg
     2101 tagacaagta gctttttata agataagttc tccaaaacaa tctgatttat aagctctttt
     2161 tcatttttcc tgtcgacaaa atacggttct ctaggtttta tctgccatat gtctttaaa
//
LOCUS       DAOSHR010000290         2216 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_48569_length_2216_cov_3.804257, whole genome shotgun sequence.
ACCESSION   DAOSHR010000290 DAOSHR010000000
VERSION     DAOSHR010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48569_length_2216_cov_3.804257"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="PLU28_10845"
     CDS             complement(<1..401)
                     /locus_tag="PLU28_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HOG35597.1"
                     /translation="MNEKIPMKNIKIGHSQSYEGATGCTVILCPEGAVAGVDVRGGAP
                     GTRETDLLDPVNMVEKVHAIFLAGGSAFGLDSAGGVMKYLEENGIGFDAGVTKVPIVS
                     GAVLFDLTCKDYKIRPDKEMGYEACLNAGNF"
     gene            complement(462..737)
                     /locus_tag="PLU28_10850"
     CDS             complement(462..737)
                     /locus_tag="PLU28_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOG35598.1"
                     /translation="MNKKDLVKALSDKMGITKKESGDFIDAFVEVVTETLAKGDDVKL
                     VGFGTFEVSDRKARKGVNPQTRKPIKIPARRVPKFKPGKELKDKVQK"
     gene            complement(936..>2216)
                     /locus_tag="PLU28_10855"
     CDS             complement(936..>2216)
                     /locus_tag="PLU28_10855"
                     /inference="COORDINATES: protein motif:HMM:NF022007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HOG35599.1"
                     /translation="NADSIIRFDRYNGYVYAVMDSKSTRVEFEKRETITPRMVCSKLG
                     INPENVRSLSIDSKTVSMDEGIMPGIFLDIKLYENTAYIMGDLSANINYPFGSTLLDI
                     LVPYQLSESYIVSYNTEKDSGTLSATQTQLMNDIKLKGKVYIQVNKIYSDEVTVYKEG
                     QNFLLKNKKVRLIDVMSACGGFAPVDRGYVNVYLKNEKVASYTEKDVFTDAAAEIPAG
                     AYVVVQPELKYSYITVLGSVSPRSIKSERPLSLVEILAGSSINWSVQDKIYIYEPGSE
                     KKEVDISRPEELSMIFVNPGSLIYIPSADTQIVYVFGQVARPGIVQYVKGFTLVDALL
                     KAGNPVSSSQLSTVYLFQNGPEQPPVVLDISQIVSSGAVKNEMNPQLKPGDIIFVPKN
                     MLTSVTEVMSNVTTFLGFINTSIDSYSKIKELFR"
BASE COUNT          608 a          506 c          365 g          737 t
ORIGIN      
        1 ataaaatttc cggcattaag acatgcctca taccccatct ctttatcggg tcttatttta
       61 taatctttac acgtaagatc aaaaagaact gctccgctaa ctataggaac ctttgtaact
      121 cctgcatcaa agcctattcc attttcctca agatacttca ttacgccgcc ggctgaatca
      181 agaccaaagg cgcttcctcc tgcaagaaat attgcatgaa ctttttctac catattcacc
      241 ggatccaaaa ggtctgtttc tcttgttcca ggcgctcccc ctctaacatc cacaccagcg
      301 actgcaccct cagggcaaag tattacagta catccggtag ctccttcata actctgactg
      361 tgtcctattt ttatattttt catcggaatc ttttcattca tatcagcaca tccttatatg
      421 taaattaaaa tcggctcaaa aagagccgat ttggtagatt attatttctg aactttatcc
      481 tttaattctt tacccggttt aaacttaggt actcttcttg ccggaatttt tattggtttt
      541 ctggtctgtg ggttaacgcc ttttctggct ttcctgtcag atacttcaaa tgtcccgaag
      601 ccgactaact taacgtcatc ccccttggca agagtttcgg taacaacttc tacaaaagca
      661 tcgataaaat ctcctgactc cttcttggtg atacccatct tatcggataa tgctttcacg
      721 agatctttct tgttcattac tacaccccct gtaattagtg taagtgacac tattgcactt
      781 aaaataccgt ttgctttgct tgacaactta attatattat aacataatct ttagtaatta
      841 tgtcaagctg tttatcggtt ttttcactga aatatacgtt tagaagccat tttaatggcc
      901 tttattactg catttcttct aaaaccccca tttattcatc gaaacagctc ttttatctta
      961 ctgtatgaat ctatggatgt gtttataaat cctaagaagg tagtaacgtt ggacataact
     1021 tctgtaactg atgtaagcat atttttcggc acaaaaataa tatctccggg tttaagctga
     1081 ggattcattt catttttaac ggctccggaa gaaacaatct gcgaaatatc aagcaccacc
     1141 gggggctgct ccggaccatt ctggaaaagg tatactgtag aaagctgcga gcttgaaaca
     1201 ggattgcctg ctttaagcaa cgcatcaaca agcgtaaagc cctttacata ctgtactatt
     1261 ccaggtcttg caacctgtcc gaatacataa acaatctgag tgtcagccga tggaatataa
     1321 atcagagaac caggatttac aaatatcatg gaaagttcct caggtcttga tatatccact
     1381 tccttcttct ctgagcccgg ttcataaatg tatattttat cctgcacaga ccagtttatg
     1441 cttgaacctg caagaatttc taccagactc agtggtcttt cgctttttat acttcgcgga
     1501 gatacagaac ccagaacagt aatgtatgag tatttaagct ctggctgaac cactacgtat
     1561 gcacctgctg gaatttcggc cgctgcatcg gtaaatacgt ctttttctgt ataagacgct
     1621 actttttcat tcttgagata gacatttaca tatcccctgt ccaccggcgc aaagcctccg
     1681 caggcagaca tgacgtctat caaacgaact tttttgtttt tcagcaggaa gttctgccct
     1741 tctttataaa ccgttacctc gtctgaatat attttattga cctggatgta tacttttcct
     1801 ttaagcttta tgtcattcat aagctgggtc tgggttgcgc tcagagtacc actgtctttt
     1861 tctgtattgt agcttactat atacgattca gacagctgat aaggaacaag tatatccaaa
     1921 agcgttgacc cgaagggata gtttatattg gcagaaagat ctcccattat atatgctgta
     1981 ttttcataaa gctttatatc caaaaatata ccgggcataa ttccttcgtc cattgaaacg
     2041 gtttttgaat ctatactgag ggacctgaca ttctctggat ttataccaag tttagaacat
     2101 accatccgcg gcgttatcgt ttctctcttt tcaaactcca ctctggtgct tttgctgtcc
     2161 ataacagcat aaacataacc gttatacctg tcaaatctta ttatactgtc tgcatt
//
LOCUS       DAOSHR010000291         2215 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_48606_length_2215_cov_3.733333, whole genome shotgun sequence.
ACCESSION   DAOSHR010000291 DAOSHR010000000
VERSION     DAOSHR010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48606_length_2215_cov_3.733333"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1668)
                     /locus_tag="PLU28_10860"
     CDS             complement(<1..1668)
                     /locus_tag="PLU28_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOG35600.1"
                     /translation="MNTLKKVLIWLFITVINVLLFWVFFTLLTFGNYGLGIVVGIFLA
                     IADWAVFSKKGYPYRYTLPAMFFLFILTIYPMYYTIKTAFTDYGTGHLFTRDQAIQTL
                     LTDPRFNYEPQEGKTLDFRVYVRYDEKFQPTEDFRILLYDNESIFVTEKLSDIRYDSK
                     GNTINANTRMYKAQDDYINLDSSSYRLIRSVSETTLEPFSARVDNISAIEDSQKIRYS
                     YFYSINDPSVAGNSAFYASVLRQKYIGNLEISYDTLRFRLSDRNIYRKFSETYKKYDI
                     LVTTENIGQRNIYKTIVVNKLASRNLTEKDGAFWDYDENGNLVRLEGYVQDIGTGQFE
                     KIFKDPKITGPFIKIFIWTFTYAGLSVLFTFAIGLMLALALNDKGLRGRMIYRTLLIL
                     PWAIPAFISVLVWRNGFFNETYGFINKFVVTPLGFEPLKWLNDPFLAKVAVLIVNTWL
                     GFPYMMTVTLGSLQSIPEELYEASSIDGASRFKQFWKITFPLLMVSVAPLLVMSFAFN
                     FNNFVNIYLLTAGNPAMANTNTYAGETDILISYTYKLAFEGGRGQDFG"
     gene            complement(1766..>2215)
                     /locus_tag="PLU28_10865"
     CDS             complement(1766..>2215)
                     /locus_tag="PLU28_10865"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOG35601.1"
                     /translation="PWAIPDYNNAGINYGVWPLASIELEKGKSLKPFAGVQGFMVNAK
                     SKNKALAKEFIINYISTTEGAYTIYKEDPRTPARKDVAEKVIATDENAKVFRDAAIGA
                     EPMPNVLEMGNIWSVMNGEGLVNFTNGTSTPEQALKDAVSKILANLK"
BASE COUNT          623 a          433 c          460 g          699 t
ORIGIN      
        1 gccgaaatcc tgtccgcgtc cgccttcaaa agctaattta taggtgtatg aaatcaggat
       61 gtcagtttct ccggcataag tgtttgtgtt tgccattgcc ggatttccgg cagtgagaag
      121 gtatatattg acgaaattat taaaattgaa agcaaagctc attaccaata ggggagcgac
      181 agagaccata agcagaggaa atgtaatctt ccagaactgt ttgaacctac ttgccccgtc
      241 gatagaggat gcttcataaa gctcttcagg tatgctctga agtgagccaa gggtaactgt
      301 catcatatat ggaaagccaa gccatgtatt tacaataagt actgccactt ttgccagaaa
      361 ggggtcatta agccatttta atggttcaaa tcccaaaggt gtaacaacaa atttgtttat
      421 gaatccgtag gtttcgttaa aaaatccgtt tctccagacc aaaaccgaaa taaatgcagg
      481 aatggcccat ggaaggataa gaagtgtcct gtatatcatt cgtcctctga gacccttgtc
      541 gttaagagcc aatgcaagca tgagtcctat cgcaaaggta aatagaacac tcaatcctgc
      601 gtatgtaaat gtccatataa atatttttat aaatggtccc gttatctttg gatctttgaa
      661 tattttttca aactgtccgg ttccgatatc ctgtacgtat ccctcaagtc ttaccagatt
      721 gccgttttcg tcgtaatccc agaatgcacc gtctttttcg gtaagatttc ttgaggccag
      781 cttgtttaca actatggttt tataaatatt gcgttggcct atgttttctg tggtgacgag
      841 gatatcgtac tttttatatg tttcagaaaa ctttctgtat atgtttcggt ctgacaaccg
      901 gaacctgagt gtatcgtaac ttatttccag attcccgatg tacttctggc ggagaacgga
      961 agcgtaaaaa gccgagttgc ccgcaacaga tggatcgttt atagaataga agtaagagta
     1021 tcggattttc tgggaatctt ctattgccga tatgttgtca actcttgctg aaaatggttc
     1081 aagagttgtt tctgagacac ttcggatgag cctataggat gaagaatcaa ggttgatata
     1141 gtcgtcttgt gccttataca ttcttgtatt cgcatttata gtatttccct tagagtcata
     1201 cctgatatca gaaagttttt ctgttacgaa tatgctttca ttgtcgtaca gaaggattct
     1261 gaaatcttcc gtcggctgga atttttcgtc atagcgcacg tatactctga aatcaagcgt
     1321 ttttccttcc tgaggttcat aattaaacct tggatcagta agcagtgtct ggatagcttg
     1381 gtcgcgtgta aaaagatggc cggtgccgta atctgtgaat gctgttttta tggtatagta
     1441 catgggatag atagtcagta tgaaaaggaa aaacattgca ggaagggtat aacggtaagg
     1501 atagcctttc tttgagaata cagcccagtc cgctatcgcc aggaaaatgc cgactactat
     1561 tcccaatccg taattgccaa aagtcaaaag tgtaaagaac acccagaaga gcagtacgtt
     1621 tataactgtt ataaaaagcc aaataagaac tttctttagc gtattcataa taacctcccg
     1681 cctttcggtc ttgggcaaaa taaaatgctt ttcttatata gaaacaaaga agggagatat
     1741 taacctccct tcttcacgga aaaacttatt ttaagttggc gagtatcttt gaaacagcgt
     1801 ctttaagtgc ctgttcgggt gttgatgtgc cgtttgtaaa gttcacaagg ccttcgccgt
     1861 tcataaccga ccatatattg cccatttcca gaacgttagg cataggttcg gctcctattg
     1921 cagcatccct gaagacttta gcattttcat cagttgcaat aaccttttct gcaacatctt
     1981 ttcttgcggg agtcctggga tcttccttat aaatagtgta agcaccttct gttgttgaaa
     2041 tataattaat tataaattct ttggcaagag ctttgttttt agattttgca ttaaccatga
     2101 aaccctgtac tcctgcaaaa ggctttaagg atttaccctt ttccagttcg atgcttgcaa
     2161 gtggccatac tccgtagttt ataccggcat tgttgtagtc cgggattgcc catgg
//
LOCUS       DAOSHR010000292         2210 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_48767_length_2210_cov_5.525290, whole genome shotgun sequence.
ACCESSION   DAOSHR010000292 DAOSHR010000000
VERSION     DAOSHR010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48767_length_2210_cov_5.525290"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..861
                     /locus_tag="PLU28_10870"
     CDS             <1..861
                     /locus_tag="PLU28_10870"
                     /inference="COORDINATES: protein motif:HMM:NF013355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOG35602.1"
                     /translation="DYMKWKKEFLPNIKNKLSVPLIVSLGYSEEELEFLIPQFEEYAD
                     IFEISTHYCSTEMTVLEKTVRTIRKNTKKPFLMKMSPHTPDPVLFAKTAKENGAGGIV
                     IMNSLGPSLKVDLKNHRVLLGNEKGQVWASGSILKSISLAMIRTVSQSVEDFDIVGTG
                     GISNAGDVLEFMLCGAKAVQLVSGALINGKDIFARIISALEPALTENGFSSIMDLINT
                     SIEPFSPEYLPKYPVIDAKRCTLCGLCERVCPYFAVKVGDSVSIDKNRCFGCGLCQSR
                     CPVKAITGVW"
     gene            864..2114
                     /gene="thrC"
                     /locus_tag="PLU28_10875"
     CDS             864..2114
                     /gene="thrC"
                     /locus_tag="PLU28_10875"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOG35603.1"
                     /translation="MKKFRFRCMNCSKLYEENEIEYTCPQCGTRLGTLETLYDFDSIT
                     VKKSDFDSCSDIWQFEALLPVSTKSFRVNLKIGFTPLYRFENLSGVKEVQIKYDGTTP
                     TASFKDRATAIAVNKAVEKNYSSIYCASTGNAAASLAGLSAAAGLKAYIFIPSKAPAA
                     KIAQLMIYNAMILAIDSDYDTAFDLSLKIGEEKNWYCRNSAINPYLIEGKKTAAMEIC
                     VQTEFDPPDTVNVPVGDGTVISSFYKGFSDFYNAGIIKKIPQLIGVQACGANSLELAF
                     KKGEPFWPVDFSGKTIADSINVGKPRDFLKACTYIKKSKGDLMSVTDDEILSAQKELA
                     SSTGIFSEPAGAASYAALKKLVGENRIEKSSRVCLAVTGNGLKDISAVSACKAIEHLK
                     PDVQDIKEYIDFKETYGDESYDEN"
BASE COUNT          734 a          391 c          477 g          608 t
ORIGIN      
        1 gactatatga aatggaagaa agagtttctg cccaatatca aaaataaact cagtgtacct
       61 ttgatagtca gtcttggcta ttcagaggaa gaacttgaat ttctgattcc gcaatttgaa
      121 gagtatgccg atatttttga gatttctact cattactgtt caacagagat gacggttctc
      181 gaaaaaactg tacggacaat aaggaaaaac acaaaaaaac catttttaat gaaaatgagt
      241 ccgcacactc cagatcctgt actgtttgca aaaaccgcaa aggaaaacgg agccggcggt
      301 atagtaataa tgaactcttt agggccttca ttaaaagtag atcttaaaaa tcacagagta
      361 cttttgggaa atgaaaaagg tcaggtttgg gcatcgggga gtattctaaa atccatatct
      421 cttgcaatga taagaactgt aagtcaaagt gtggaggatt ttgatatagt cggaacaggc
      481 ggtataagta atgcgggaga tgttcttgaa tttatgcttt gcggagctaa ggcggtacag
      541 cttgtctcgg gcgcgcttat caatggaaaa gatatctttg ccagaatcat atctgctctt
      601 gaaccagcac tgacagaaaa tggctttagc agtatcatgg atcttataaa tacttctatt
      661 gaaccatttt caccagaata tcttcccaaa tatccggtta tagatgccaa acgctgtact
      721 ctttgcggac tgtgtgaaag agtttgtcct tactttgcag ttaaagtcgg tgatagtgta
      781 agtatagata aaaacagatg ctttggctgc gggctgtgtc agtcaagatg tccggtaaag
      841 gccataacgg gagtgtggta aagatgaaaa aattcagatt ccgctgtatg aactgttcaa
      901 aactatatga agaaaacgag atagagtaca cgtgtccaca atgcggtaca aggctcggaa
      961 cacttgaaac attatatgat tttgacagta ttacagtgaa gaagagtgat tttgacagtt
     1021 gctctgatat atggcagttt gaagcactgc tgcctgtaag tactaaaagc ttcagagtta
     1081 atctaaaaat aggtttcaca cctttgtacc gttttgaaaa tctttcaggc gtgaaagaag
     1141 tacagataaa gtacgacggt acgactccca cagcttcttt taaagacaga gcaacagcta
     1201 ttgctgtaaa caaagcggta gaaaaaaact attcaagcat ctactgtgct tcaaccggga
     1261 atgcggcggc atctcttgcc gggttaagtg ctgctgccgg attaaaagca tatattttta
     1321 taccaagtaa agctccggca gcaaaaattg cccagcttat gatatataat gccatgattc
     1381 ttgcaatcga ttctgattac gatactgctt ttgatctttc tcttaagata ggcgaagaaa
     1441 aaaactggta ctgccgtaac tccgccatca atccgtatct catcgaaggc aaaaaaacag
     1501 ctgccatgga gatatgtgtt cagactgagt ttgatcctcc cgacacagtt aatgtacccg
     1561 taggtgacgg aacggttata agctcatttt ataaaggttt ttctgatttt tacaacgcag
     1621 ggataataaa aaaaattcca cagcttatag gtgttcaggc gtgcggggct aacagtcttg
     1681 aacttgcttt caaaaaaggc gaacctttct ggcctgtgga tttcagcggt aaaaccatag
     1741 cagacagtat aaacgtgggt aaacccagag attttttaaa agcctgtaca tatataaaaa
     1801 aatcaaaagg tgacttaatg tctgttacag atgatgaaat actttcggct caaaaagagc
     1861 ttgcttcaag cacgggtata ttttctgaac ctgccggagc agcttcatat gcagctctaa
     1921 aaaaattggt aggcgaaaac agaatagaaa agagttcacg agtatgtctt gcggttaccg
     1981 gaaatggcct gaaagatata tccgcagtat cggcatgcaa agctatcgaa catctgaagc
     2041 ctgatgttca ggatattaaa gagtatatag attttaagga aacatatgga gatgagagct
     2101 atgatgagaa ttaaagagta tttaaagcct gaaaatcttg acaaagcgta tacagctctc
     2161 aaagaatctc caaataatga aataatcgca ggagctcttt ttataagact
//
LOCUS       DAOSHR010000293         2187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_49530_length_2187_cov_3.228424, whole genome shotgun sequence.
ACCESSION   DAOSHR010000293 DAOSHR010000000
VERSION     DAOSHR010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49530_length_2187_cov_3.228424"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1397
                     /locus_tag="PLU28_10880"
     CDS             <1..1397
                     /locus_tag="PLU28_10880"
                     /inference="COORDINATES: protein motif:HMM:NF024947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HOG35604.1"
                     /translation="ADTASLLAIKERLSADITETNTQKEDTEHEIILILERNSSSSAE
                     HFIKSIKEKDQKFAELDKQMSESTRILKEEGLKESSVMKNLSEITSKISELSAKLDEK
                     SIQLKALNEEMTARPAWSKDPYYVLSNLEIQKKQIETRYTKLEESFSQLRKNYENTNT
                     ELHSIIKTLETYSKMLSEKETLLTAQMKEKGFSDIQDIKNYYRSQNKLEQMQEQISTH
                     EKELAISEKTVKNLSEKITDRTLTYDDLQKKNEVLSAVKKEYEALSGEIATTESKIKE
                     MSEKLTEKTDMEKEYGLIKKEADTAELLNKKLRGNEFVKYVSSLMMENILSAASSQLR
                     AITNNRYSLETDESAFIIRDDFNGGYRRRVDTLSGGEIFLASLCLALALSAHIQLKGK
                     KSIRFFFIDEGFGSLDSSLIDTVMSSLEKIQQKHLCVGLISHVEEMKERIQSKLIIYK
                     QETENGITSKIDII"
     gene            1527..>2187
                     /locus_tag="PLU28_10885"
     CDS             1527..>2187
                     /locus_tag="PLU28_10885"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HOG35605.1"
                     /translation="MRFHFSPNLFVLYFSFFISLFLGVWGIKNRKKPAALQFGLSMLC
                     LTLWTFGFILELAGIDFKTKLIWYNFQFLFIYLFPVNLFWMTKDLSNLWLKEKLERFY
                     FYIFISISLIIFLTGVINPHDSLFMKNLSIIENNGYTFLAGAAGPVYYIGIGYVFFFV
                     ILSVGILLYELIKREKFFKLQILLLLLAQVFPLITSVLYTFKLLDTSFNYSTFIFSLS
                     GI"
BASE COUNT          789 a          416 c          373 g          609 t
ORIGIN      
        1 atgctgacac agcttcactt ttggcgataa aagaacgcct ttcagcagac ataaccgaaa
       61 ccaacacaca aaaagaagat actgaacatg aaatcatact cattcttgaa agaaactcat
      121 cttcttcagc agaacatttc ataaaaagta ttaaagaaaa agatcagaaa tttgcggagc
      181 ttgataagca gatgtcagaa agtaccagaa tacttaagga agaaggtctc aaagagagtt
      241 ccgttatgaa aaacttatcc gaaataactt caaaaataag cgaactatca gcaaaactag
      301 atgaaaagtc catacagctt aaagctctta acgaagaaat gacagcccgt cctgcatgga
      361 gcaaagatcc ttactatgtt ctgtctaacc ttgaaataca aaaaaagcag atagaaacac
      421 gctataccaa gcttgaagaa agtttttcac agcttcggaa aaactatgaa aacaccaaca
      481 cagaactaca ttcaataata aaaactcttg aaacatactc taaaatgctt tcagaaaaag
      541 agactctact tacggctcag atgaaagaaa aaggattttc agatattcag gacataaaga
      601 attattacag atcacagaat aagcttgagc agatgcagga acaaatctca acacatgaaa
      661 aagagcttgc catatcagaa aaaaccgtaa aaaacctgtc agaaaagatc accgaccgaa
      721 ctctcaccta cgatgacctg caaaagaaga atgaagtact ttcagcagta aaaaaagagt
      781 atgaagcatt aagcggagag atcgcaacaa ccgaatcaaa aataaaggaa atgtcagaaa
      841 agcttacaga aaaaacagat atggaaaaag agtacggact gattaaaaaa gaagcagata
      901 ctgctgaact gctcaacaaa aaattaagag gaaatgaatt tgtaaagtat gtttcttcgc
      961 ttatgatgga aaacatactt tcagcagctt cctcacagct cagagccata accaacaaca
     1021 gatattcgct cgaaacagac gagtcggcct tcataatacg cgatgatttc aacggaggat
     1081 acagacgaag ggtagataca ttatcaggcg gagagatctt tcttgcttca ctatgccttg
     1141 ctcttgccct ttcagcccat atacagctga aaggaaaaaa gagcataaga tttttcttca
     1201 tcgatgaggg ctttggatct ttggacagca gccttattga tactgtcatg tcttctcttg
     1261 aaaaaataca gcagaagcat ttatgcgtcg gactcataag tcatgtagaa gagatgaagg
     1321 aaagaataca atccaaattg attatttata aacaagaaac agaaaatgga ataacaagta
     1381 aaatagatat aatataatct ttttttatta tgttcataca aaaaaagaaa agctgcaaaa
     1441 cacataaagt aatttgtttt tcttctttta cttgtgcggt ggtataatta aatatataac
     1501 ttaaaaaata tataacaggg agtgctatga gatttcattt cagcccgaac ctatttgttc
     1561 tctattttag cttctttatt tctctttttt tgggagtatg gggtatcaaa aacagaaaaa
     1621 aaccggctgc ccttcaattc gggctcagca tgctttgtct taccctttgg acatttggtt
     1681 ttattctgga gcttgccggt attgatttta aaaccaagct tatctggtat aactttcagt
     1741 tccttttcat atacctcttt ccagtaaacc ttttctggat gaccaaggat ctgtcaaacc
     1801 tttggcttaa ggaaaaactt gaacgatttt atttttatat tttcataagc atttctctga
     1861 tcatctttct gaccggagtt ataaatcccc acgacagcct ttttatgaaa aacctttcta
     1921 taatagagaa caatggatat acctttctgg caggagccgc aggaccggtt tactatatcg
     1981 gtataggata cgtttttttc tttgtcatac tctctgtagg catccttctt tacgagctga
     2041 taaaacgcga aaagttcttt aagcttcaga tactgctttt gctcctggca caggtattcc
     2101 cgcttataac ctctgtgctg tacaccttta aactgcttga tacctctttt aactacagca
     2161 cgtttatctt ttcactttcc ggaatac
//
LOCUS       DAOSHR010000294         2176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_49875_length_2176_cov_4.723244, whole genome shotgun sequence.
ACCESSION   DAOSHR010000294 DAOSHR010000000
VERSION     DAOSHR010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49875_length_2176_cov_4.723244"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /gene="atpB"
                     /locus_tag="PLU28_10890"
     CDS             <1..617
                     /gene="atpB"
                     /locus_tag="PLU28_10890"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HOG35606.1"
                     /translation="ASTVKFRLVPEKERQTVFELLFEFYWSIVEDAVPNPKYRKPVFI
                     LGVSFFLYVLVSNLLGGMPGISVSPLSNGKVSVEMFTDTWYTPTSDLNVNAAYAISVL
                     IISHVFSAHSKGVWKWFKGFFEPIVFLLPLNIIGEIAKPISHSVRLFGNIMGGGILVL
                     IVSYMLKYFLIPVFLWGFFGWFVGLIQAFVFSLLAIAYIGSAVE"
     gene            645..944
                     /gene="atpE"
                     /locus_tag="PLU28_10895"
     CDS             645..944
                     /gene="atpE"
                     /locus_tag="PLU28_10895"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HOG35607.1"
                     /translation="MENYSIVEALKLVLASGNMLAWGLYYMGKFIGTGICMGIGAIGP
                     GVGEGETGAHAMDAMARQPEMMGSIRINMLLAMAVEESTGLYSLVISLIMLFVLP"
     gene            982..1476
                     /gene="atpF"
                     /locus_tag="PLU28_10900"
     CDS             982..1476
                     /gene="atpF"
                     /locus_tag="PLU28_10900"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HOG35608.1"
                     /translation="MINFNLTSIVNLVGFIFFVLILYKLLYKPFLDVVAKRKEIISKS
                     LSDAEKAKLDSEKIKEDMEKEIKDLKSARDEKILQAEAQAKAIVDEAKKQALVEKERI
                     ILKAEQEASELKEKAALEIQSNVVRLAVTISAMILKEKVDVKANEELARRAMQALNNK
                     GESL"
     gene            1473..2027
                     /gene="atpH"
                     /locus_tag="PLU28_10905"
     CDS             1473..2027
                     /gene="atpH"
                     /locus_tag="PLU28_10905"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HOG35609.1"
                     /translation="MKASYSLAFRYSKAFVEFLSQNNKINALDSYIEILKTLDKKIQQ
                     DEEFSNLFKNPVISQKHVLNKTLEYLDNSGDEILSKFLSLIIENKRQELLANIIRHLR
                     VHSLNMKRLVRVKLTTAKELSNETKDLIFETITKKTGRKISISYTLDESLIGGIQMEF
                     DDKLFDYSVKGYLDSIIRDVSSRG"
BASE COUNT          702 a          354 c          483 g          637 t
ORIGIN      
        1 ttgccagtac cgtaaagttc agactcgtac cggagaagga acgtcagacg gtattcgaac
       61 tgctctttga attttattgg agtatagtcg aagatgctgt tccgaatccc aagtacagaa
      121 agcctgtttt catattaggt gtttcttttt ttctttatgt tctggtttca aatcttttgg
      181 gaggaatgcc cggaataagt gtttcacctc tttccaacgg caaggtatca gttgagatgt
      241 tcacagatac ttggtatact ccaacctcgg atctgaacgt gaatgctgcg tatgctattt
      301 ccgtgcttat tatcagccat gtgttttctg cccattccaa gggcgtatgg aaatggttta
      361 aaggtttttt tgaaccgata gtgtttcttc ttccgttaaa tatcatagga gagattgcaa
      421 aacccatatc acattcggta aggctttttg gtaatattat gggcggaggg atacttgtgc
      481 ttatagtcag ctatatgctt aagtacttcc ttattcctgt ttttctctgg ggattcttcg
      541 gatggtttgt cggtctcata caggcattcg ttttttcttt gttggcaata gcatatatag
      601 gttcagcagt agaataaaaa agactaaggg aggtcagata agttatggaa aattacagca
      661 ttgtagaagc cttgaaattg gttttagcaa gcggcaacat gctcgcatgg ggattatatt
      721 acatgggtaa gttcatagga accggaatat gtatgggtat aggagctatc ggtcctggag
      781 tcggtgaagg agaaaccgga gcacacgcta tggatgctat ggcaaggcag cctgaaatga
      841 tgggttcgat aaggattaac atgcttttgg ctatggctgt agaagagtct acagggctgt
      901 attcacttgt tatttctctc atcatgcttt ttgttcttcc ttaaaaaagg aaggaaatct
      961 tcctgaaagg aggagatcgc tttgataaac tttaatttga catcgattgt aaatctggtt
     1021 ggatttatat tttttgtact tattctgtac aagcttcttt acaaaccttt tctggatgtt
     1081 gtggccaaaa gaaaggaaat aatttccaaa agccttagcg atgctgaaaa agccaagctc
     1141 gactccgaaa agataaaaga agatatggaa aaagaaataa aggatcttaa gagtgccaga
     1201 gatgaaaaaa tccttcaagc tgaggcgcaa gcaaaagcta ttgttgatga ggcaaagaaa
     1261 caggccttgg tggaaaagga gagaatcata ttaaaagccg aacaggaagc ttctgaactt
     1321 aaagaaaagg ctgcactcga gattcagtcg aatgttgtaa ggcttgcagt taccataagt
     1381 gcaatgattc ttaaagaaaa agttgatgtg aaagccaacg aagaacttgc caggagggct
     1441 atgcaggctt tgaacaataa aggggaatca ttatgaaggc ttcctactct ttagctttca
     1501 gatattcaaa agcctttgtc gagtttcttt cgcaaaacaa taagataaat gcacttgaca
     1561 gctatataga gatattgaag acactggata aaaaaatcca gcaggatgaa gagttttcaa
     1621 atcttttcaa aaatcccgta atatctcaaa aacacgttct aaacaagact cttgaatatc
     1681 ttgacaatag cggtgatgag atcctgagca aatttttatc tcttattata gaaaataaaa
     1741 ggcaggagct tcttgccaat ataataaggc atctgagagt acacagtctc aatatgaaaa
     1801 gacttgtccg tgtaaaactt acaacagcca aagagctttc aaatgaaacc aaagatttga
     1861 tttttgaaac aataaccaag aaaacgggaa gaaagataag catatcttat acacttgatg
     1921 aatccctgat cggaggtatt caaatggagt ttgacgataa gcttttcgat tattccgtta
     1981 aaggttatct tgacagcata atcagagatg tttcctcccg ggggtgaaat actttgcaaa
     2041 taaatcctga agaacttaca aaagtcttaa gtgaaaagat aaaagactat gaaagtgctg
     2101 aaataaagga agtaggatgg ataatgcagg taggagacgg aattgtccgt gcctacggca
     2161 tgaaagatgt aatgta
//
LOCUS       DAOSHR010000295         2172 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_50021_length_2172_cov_5.514880, whole genome shotgun sequence.
ACCESSION   DAOSHR010000295 DAOSHR010000000
VERSION     DAOSHR010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50021_length_2172_cov_5.514880"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(127..1572)
                     /locus_tag="PLU28_10910"
     CDS             complement(127..1572)
                     /locus_tag="PLU28_10910"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOG35610.1"
                     /translation="MICYELFLRSFFDGNGDGIGDLDGLEHKLDYLIDLGVDCIWLLP
                     IMSSPAFHGYTVSDFQSINSLYGNLDDLHKLMSSAHSKGIKIILDIPVNHVSVCHEWF
                     VKALQGESKYEKCFTWADDKTDINELRHWGNARIWHNIDNKHFYGLFGPGSADLNFEN
                     PFVVEEIKEIFSFWLEKGFDGFRLDAAKHIFDYNTQKMKFEYQHEKNIAFWEDMRDYI
                     LGINKDAVIISEVWDSPEIVKKYEGLFEIGFNFPFSYSIKDSVKNNDASFLADEMQKN
                     LSSYFADSKVVTKSGNFLTNHDMTRLYSELGNIKDVKFAFSILFTLPGIPFIYYGEEL
                     AMQGKLKSVDFTEDSQEPMQWYELGYGYGQTEWKGYKFNPVGTGRSVQAQLQDSNSFL
                     SFMKQLILFRKDNLWLEDAKIQIIARSKKSLTLKLYCSKKEALVIYNFSSVKKVLCEE
                     KYKIFYVSNCQKKGKEVIMRPKSVLVIIKKS"
     gene            1771..>2172
                     /locus_tag="PLU28_10915"
     CDS             1771..>2172
                     /locus_tag="PLU28_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35611.1"
                     /translation="MKKTVFLSLLALLFIFALSSCNIKTLEIKAKPTLSIPAGATSVE
                     FSGYLNEIEKSITDSLPQATVTSKNPITIGFSKDLIDLDTNEFFNSSEINFAQVNQTF
                     SASFTVPTIPEFNPISLELPSISAEPLDLSEQ"
BASE COUNT          683 a          457 c          349 g          683 t
ORIGIN      
        1 atttagaaaa gtgaaagaac ttgaagcaaa atcaaaagca caaggcacat ccctcccggc
       61 tacgccgtac tcccctgaag gggaaaataa acccgagaat ctttttacag actcccgggt
      121 tgggttttat gattttttta taatcacgag aacagatttg ggtctcataa taacttcttt
      181 tccttttttt tggcagttgc tgacatagaa aattttatac ttttcctcac aaagtacttt
      241 ttttaccgaa gaaaagttat aaatgactaa tgcctctttc tttgagcaat aaagttttaa
      301 ggtaagactt ttctttgatc ttgcgattat ctgaatcttt gcatcttcaa gccatagatt
      361 atcttttctg aaaagtatta gctgcttcat aaaagataaa aatgagttac tgtcctgcag
      421 ctgtgcctgg actgatcttc ctgtaccaac cggattgaac ttgtacccct tccattcagt
      481 ctgcccgtaa ccataaccca gctcgtacca ctgcatcggc tcttgtgaat cttcggtaaa
      541 atcaacgctt ttaagtttcc cctgcattgc aagctcttca ccgtaatata tgaacggtat
      601 tccgggaagt gtgaaaagaa tggagaaggc gaattttaca tccttaatgt tccccaactc
      661 gctgtaaagt ctggtcatat catgattggt cagaaaattt ccagatttcg tcacaacctt
      721 agaatctgca aaataagagg aaagattttt ttgcatttca tctgcaagaa atgaggcatc
      781 gttatttttt acagaatcct ttatactata tgaaaaagga aagttaaagc ctatttcaaa
      841 aagtccttcg tattttttca caatttcagg actgtcccat acttcgctta taataacagc
      901 atctttattt attcctaaaa tataatcccg catatcttcc cagaacgcta tatttttctc
      961 atgctggtat tcaaacttca ttttttgtgt attataatca aagatatgct ttgcggcatc
     1021 aaggcggaac ccgtcgaatc ctttttcaag ccagaatgaa aagatctctt ttatttcctc
     1081 tacaacaaac ggattttcaa agttgagatc tgcagatcct ggtccaaaaa gaccgtagaa
     1141 atgcttatta tctatgttgt gccatattct tgcatttccc caatgacgaa gctcgtttat
     1201 atctgtttta tcgtcagccc atgtgaagca tttttcatat ttactctctc cctgcaaggc
     1261 cttgacaaac cattcatgac atacggaaac atggttcacc ggaatgtcaa gaattatctt
     1321 tataccttta ctgtgagctg aagacatcaa tttatgaaga tcatcaagat tcccataaag
     1381 actgttgata ctttgaaaat ctgacacagt atacccgtga aatgcaggcg aactcattat
     1441 aggcaaaagc catatgcagt ctacgccaag atcaataaga taatccagct tgtgctccaa
     1501 accatccaaa tctcctattc catcaccatt tccatcaaaa aaggaccgta aaaaaagttc
     1561 atagcatatc ataagtatgc ccccttaaga taattataat cagattatac aataaaatcc
     1621 ggacaagcgg gaatttttca gtgataactc gacgcatatc caacctgaaa agtttaaaaa
     1681 gtatttgtta acatgatata attatattaa tgacaatcat tattaacaaa aaacctatta
     1741 tttttttgca aaaaactctg gaggtggatt atgaaaaaga cggttttcct ttcactgctt
     1801 gcattactgt ttatttttgc gctttcgtcc tgcaatataa aaactttgga aataaaggcg
     1861 aagccaaccc tgagtattcc tgcgggagca acatcggttg agttttcagg atatttgaat
     1921 gaaatagaaa aaagcatcac cgattcgctt ccacaggcaa cagtcacttc taagaatcca
     1981 atcaccatag gtttttcaaa agatctcatc gatcttgata ccaatgagtt tttcaacagc
     2041 agcgagatta attttgcaca ggtaaatcaa accttcagtg caagctttac cgttccgaca
     2101 ataccggaat ttaatccgat cagtctggaa ctgccttcca tttcagccga acctcttgac
     2161 ctttcggaac ag
//
LOCUS       DAOSHR010000296         2169 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_50123_length_2169_cov_4.052034, whole genome shotgun sequence.
ACCESSION   DAOSHR010000296 DAOSHR010000000
VERSION     DAOSHR010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50123_length_2169_cov_4.052034"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="PLU28_10920"
     CDS             <1..377
                     /locus_tag="PLU28_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35612.1"
                     /translation="RDVKKGAELLGDIIFVHEHNKSVYIVSSAGEVVVLGRKQALVPW
                     DLLRAPFSIELSEKGIEIKTMQLKKYIMSFEGGLSSSQLYEDIKFNIPLFKPLDTYKF
                     EDFEIKIYSSYIKILRPEVKAK"
     gene            374..1147
                     /locus_tag="PLU28_10925"
     CDS             374..1147
                     /locus_tag="PLU28_10925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HOG35613.1"
                     /translation="MIDGKVRLDIFLSLKDARISRKSANSLIRQGKVTVNGKVVLKPS
                     FQVNEEDTIIVLSEEFYVSRGAFKLQKALEEFEVQVRGKVCMDIGSSTGGFTQVLLRN
                     DALRVICVDSGTDQLHPLLRNDARVILYENTNARYFKADEKVDFICCDVSFISITKLT
                     DTFLENSKDTAEFIFLVKPQFECGKEFLGKNGIVKDRQVHSSVLKNVSAHLNYVGLFP
                     NRVIESPIKGSQGNTEFLLYCKRNKSVAFDMKNITGAVE"
     gene            1149..>2169
                     /locus_tag="PLU28_10930"
     CDS             1149..>2169
                     /locus_tag="PLU28_10930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35614.1"
                     /translation="MKKRIGKINISMIFFLLYSIIGYSYTSVISIDGLGRFYKNCLVN
                     IPLFRYINSKEGLGYEYIFNVLSETLSCPKEDLENLLESRVTFYSDFEYNYSAFLEPD
                     IIKNLSNIILRSDAIYIENHDVKTISDIIENIFNGTVVKGGYDIYGKEYRISYGENGV
                     FLNRPQSVYTPSGEYPVEGKIKGFMNDGKNWDFHLEIKDMALKGSFVSQDYSKDMQEF
                     DISLLENWRIFGDVQTYEVLVKDRTVDYIREFFVPYDKSSIEIIDKLFSSPKDGQFVV
                     IRSESLTDSKMRSIYFSTVFDTKNINALIKLYNLGSGKIGTYDYCLISHKSVGDKIYF
                     YYSQEG"
BASE COUNT          732 a          330 c          447 g          660 t
ORIGIN      
        1 tacgtgatgt gaaaaaaggg gctgagcttc taggagatat tatatttgtg catgaacaca
       61 acaaatcagt ttacattgta agctccgcag gagaagtggt tgttcttggc agaaaacaag
      121 cccttgtacc atgggatctt ttaagagctc ctttttccat agaactctct gaaaaaggga
      181 tagaaataaa aacgatgcag ttgaagaagt atataatgag ttttgaaggc ggactttcat
      241 cgtctcaact gtatgaagat ataaaattta atattccctt gtttaaaccg cttgatacat
      301 ataaatttga agattttgaa ataaaaatat acagctcgta tataaaaatt cttagacctg
      361 aggtaaaagc gaaatgattg atggtaaagt gcgtctggat atatttttaa gcttgaaaga
      421 cgccagaatc tcaagaaaaa gtgccaatag tcttatcagg caaggcaaag taaccgtcaa
      481 cggcaaagtt gttttaaaac cttcttttca agttaacgaa gaagatacta taatagtgct
      541 atccgaagaa ttttatgtca gcagaggagc ctttaagctt cagaaggctc ttgaagagtt
      601 tgaagtgcaa gtgcgaggta aggtatgtat ggatataggt tcttccaccg gagggtttac
      661 gcaggttctt cttcggaatg atgccctcag agtaatatgt gtcgacagcg gaaccgatca
      721 gctccatcct ttgctcagga atgatgctag agttattctt tacgaaaata ctaatgcccg
      781 atactttaaa gctgatgaaa aggttgattt tatatgctgt gatgtatctt ttatatctat
      841 aaccaagctt accgatacat ttcttgaaaa ttcaaaggat accgcagagt ttatttttct
      901 ggtgaaacct cagtttgaat gcggaaaaga atttttgggt aaaaacggaa ttgtaaagga
      961 cagacaagtc catagctcgg tattaaaaaa cgtttctgct catcttaact atgtagggct
     1021 ttttcccaac agagtaattg aatctccgat aaaaggttct caggggaata cagaatttct
     1081 tctgtactgt aaaagaaaca agagtgttgc ttttgatatg aaaaatataa ccggagcagt
     1141 tgaatagtat gaaaaaaaga ataggcaaga taaacatttc aatgattttt ttccttttat
     1201 acagcataat aggttattct tatacatccg taataagtat agacggactg gggcggtttt
     1261 ataaaaactg tctggtcaac atacctctct tcaggtatat taactcaaaa gaaggactcg
     1321 gatatgagta tatttttaat gttctttcag agactctttc ctgtccgaaa gaagatcttg
     1381 aaaatcttct tgaaagcagg gtaacttttt attctgattt tgagtataat tattcggctt
     1441 ttttggaacc ggatataata aaaaatctca gtaatattat actgagatct gatgccatat
     1501 atatcgaaaa tcatgatgta aaaaccataa gcgatataat tgaaaatata ttcaacggta
     1561 cggtggttaa aggcggatac gatatatacg gtaaggaata cagaatatct tacggcgaga
     1621 acggggtctt tcttaatagg cctcaaagtg tttatacacc atcgggagag tatcctgttg
     1681 agggaaaaat aaaaggattc atgaatgacg gcaaaaactg ggactttcat ctggagataa
     1741 aagacatggc tctgaaagga agttttgtat cccaggatta ttcaaaagat atgcaggaat
     1801 ttgatatatc cttacttgaa aactggagaa tcttcggcga tgttcagaca tatgaagttc
     1861 ttgtaaagga caggactgtc gattacattc gggaattttt tgttccctat gataaaagct
     1921 ctatagagat aatcgataag cttttttcat ctccaaaaga cggacagttt gtggtcataa
     1981 gatctgaaag tcttactgat agtaagatga gatcaattta tttttcgacg gtatttgata
     2041 cgaaaaatat taatgcgctc attaagctgt acaacctggg cagcggtaag ataggtacct
     2101 atgactactg tctcataagt cataaatccg tgggagataa gatatatttt tactacagcc
     2161 aggaaggaa
//
LOCUS       DAOSHR010000297         2149 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_50815_length_2149_cov_3.876791, whole genome shotgun sequence.
ACCESSION   DAOSHR010000297 DAOSHR010000000
VERSION     DAOSHR010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50815_length_2149_cov_3.876791"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..>2149
                     /locus_tag="PLU28_10935"
     CDS             23..>2149
                     /locus_tag="PLU28_10935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HOG35615.1"
                     /translation="MDDSLYTVRRSFKRSAESIITDSAVLTRNSLAVAEKAKNVDSYI
                     IQTIGLQAKDFTKCVMLPQGQFNEFLTSKGRDRSEILEKIFDLQQYGSILQQKAASYF
                     NELKMRRSVIESKLSLYTNISVQNIVTLKEKSNELKKKKELLSVQSEQLEQQSALIRS
                     QLQDLETLRKEEKRLKELMTQKEYVRSLKTTADKALAASQVFPVLEQCEKLSSSISQT
                     TGEKNDHEKTLGELKIQLDKMEKEYLDSKQQYQEKLPQIIADCENIKSFIEKKTQAQK
                     IEKECLSLKEKLSEAQTRKESLNLELSKIEETKKHLNDLLEKTEAGRKIFLVLPQQRQ
                     DNDTINHLTLKVSSLAMNGEKLSREIKEAEIRLESIEKSLDMLKQKEKLCEEQLQQKQ
                     QKLITLKNEAEDIKKKNYLAFIMKDLKDGDPCPICNGIFHPSHTDHSSTTDLRIAEIE
                     YNCNVTEKSILELLNEQLKIKEQLSALNADTASLLAIKDRLAEDIIEISKQKEDIKHE
                     IIIILERNSSSSAEHFIKSIKEKDQKFAELDEQMSNNSRMLKDEGLKEGSVLEKLSDI
                     TSKISELSAKIDEKSIQLNSLNSELTARPEWNKDPYSVLSDLDIQRKQIEDRYNELEK
                     SISQLRKKYENTNTELHSIVKTLETYSEMLSEKETLLTTLMKEREFSDLQDIKSFYRS
                     QNDIQQMKEKISTHEKELAISEKTVKI"
BASE COUNT          855 a          406 c          382 g          506 t
ORIGIN      
        1 cttcatctgt tttgaatttg aaatggatga ttccttatat acagtaagaa gaagctttaa
       61 acgaagtgca gagtctatca taacagattc tgctgtactt accagaaatt cgctcgcagt
      121 agccgaaaaa gcaaaaaatg tcgactccta tataatacag accataggcc tacaggccaa
      181 agactttacc aaatgcgtca tgctgccgca gggacagttc aatgaatttc tcacctccaa
      241 aggacgtgac agatcagaaa tcctagaaaa aatctttgat ctacagcagt atggcagcat
      301 tctgcagcag aaggccgctt catacttcaa tgaattgaag atgcgcagat cggttataga
      361 atcaaaactt tcactataca caaacatatc tgtccaaaat atcgtaactc ttaaagaaaa
      421 aagcaacgaa cttaaaaaga agaaagaact gctttcggtt caatcggaac agcttgagca
      481 acagtcagct ttaatccgtt cacaactaca ggatctggaa actttacgaa aggaagaaaa
      541 aagacttaaa gagcttatga cccaaaaaga gtatgtccgt agtttaaaaa caactgcgga
      601 taaggcttta gcggcatcac aggtttttcc ggttctcgaa caatgcgaaa aactgagtag
      661 ttcgataagc caaaccaccg gggaaaaaaa cgatcatgaa aaaactcttg gagagcttaa
      721 gatacagctt gataaaatgg aaaaggagta tcttgattca aaacagcaat atcaggaaaa
      781 acttcctcag ataatagcag actgcgaaaa tattaagagt tttattgaaa aaaagactca
      841 ggcgcaaaaa atagaaaaag aatgcttatc gctgaaagaa aagctttctg aggcacaaac
      901 ccgtaaagaa tctcttaatt tggagctgtc taagattgaa gaaaccaaaa aacatctgaa
      961 cgaccttctg gaaaaaacag aggcaggaag aaaaatcttt ttggttctgc cccagcaaag
     1021 acaggataac gatacgataa atcaccttac actaaaggtt tcgtctttgg ctatgaacgg
     1081 agaaaaactg tctcgagaga ttaaagaagc tgagatacga cttgaaagta tcgaaaagtc
     1141 cttagatatg ctcaaacaga aagaaaaact ctgtgaagaa cagcttcagc aaaaacagca
     1201 aaaacttatt acgcttaaaa acgaagcgga agatataaaa aagaaaaact atcttgcttt
     1261 tatcatgaag gatcttaaag acggagatcc ttgtcccata tgtaacggca tctttcatcc
     1321 atctcataca gaccattcat cgaccactga tcttaggatt gctgaaattg agtacaactg
     1381 caatgtcact gaaaaaagca ttttagagct tttaaatgaa cagcttaaga taaaagaaca
     1441 gctttctgcc cttaacgcag acacagcatc acttttagcc ataaaagatc gacttgctga
     1501 agacataatc gagatcagca aacaaaaaga agatattaaa cacgaaatca taattattct
     1561 tgaaagaaac tcatcttctt cagcagagca ttttataaaa agtataaaag aaaaagatca
     1621 gaaatttgca gagcttgacg aacagatgtc aaacaactcc agaatgctta aagacgaagg
     1681 actcaaagaa ggctccgttt tggaaaaatt atctgacatt acttctaaaa taagtgagct
     1741 atcagcaaaa atagatgaaa aatctataca gcttaactca cttaacagtg agctgaccgc
     1801 ccgtccagag tggaacaaag atccttactc tgttttgtct gaccttgata tacaaagaaa
     1861 gcagatagaa gatcgctata atgagcttga aaaaagcatt tcacagctta gaaaaaaata
     1921 tgaaaatacc aacaccgaac ttcattcaat cgtaaaaacc cttgaaacct actccgaaat
     1981 gctttcggaa aaagagactc tacttactac gctgatgaaa gaaagggagt tttcagatct
     2041 tcaggatatt aaaagcttct acagatccca aaatgacatt caacagatga aagaaaaaat
     2101 ttcaacacat gaaaaagagc ttgccatatc agaaaaaaca gtaaaaatc
//
LOCUS       DAOSHR010000298         2133 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_51430_length_2133_cov_5.270452, whole genome shotgun sequence.
ACCESSION   DAOSHR010000298 DAOSHR010000000
VERSION     DAOSHR010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51430_length_2133_cov_5.270452"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..716
                     /locus_tag="PLU28_10940"
     CDS             <1..716
                     /locus_tag="PLU28_10940"
                     /inference="COORDINATES: protein motif:HMM:NF024983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOG35616.1"
                     /translation="NDDIEFPTFKIIRKIRKELSDMKNNRDLTQAKQKLEQISIQLSE
                     IVSMHVYKEHSVLFPTALKLISEEEWKDIRTQFDEIGYAGYTPDKFSFVSSQDTAITN
                     DSVKLPSGELSLKQLIGIFDTLPVDITFIDSQDSVRYFSESKDRIFVRSRAIIGRHVQ
                     NCHPEKSVHIVNQILSDFKSNKRDSADFWLNIGGKTIYIRYFAVRDTQGQYLGTLEVT
                     QDIAPIKKISGEKRIYSHE"
     gene            complement(733..>2133)
                     /locus_tag="PLU28_10945"
     CDS             complement(733..>2133)
                     /locus_tag="PLU28_10945"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG35617.1"
                     /translation="DTLISFFSHRLLYSGIKKLRLTMLKKIMETKFTYFIDNSPGKIW
                     GDIINNTNKASVFYQALILLPISLIQAVVYFVIIYRINTSSAMVTLMTLPFIILATYF
                     FGIKIRKHEAKTMSMYREMYGDSVEALTGIKNIKLFNSHGFFYDRFISKHSSLNKEIV
                     STAVYTQSWQSFLKVVTNLLPLIIIVLGINFVKEFNLGSGDMVILYTFIPLFVSATKD
                     LYSFILSFFGMKPYMQLINSILSLENEDCGNIKELNGFDVQTNGVSYGFKGKTVQIPD
                     ISIGKGEKILITGESGIGKSTFFNCLTGILQDYGGSIKVSGTEQKNISLKYLRDKIIL
                     LNQDRIVFDGTVDENLFFGKPYTDVNKVENIMRIVHLEKFYSDRKDGKKISKETISGG
                     EKARLNLAQALLKNPEIILLDEVLSSVDEDMEKEILQNIITAYPKLSVVMITHRMSCR
                     DMFDKVYDFHYESTGK"
BASE COUNT          664 a          451 c          377 g          641 t
ORIGIN      
        1 ccaacgatga catagagttt cccacattta aaattataag aaagatacga aaagaacttt
       61 ccgatatgaa aaataacagg gacctcacgc aggccaaaca gaagcttgaa cagatcagca
      121 tacagctgag cgaaatagta tccatgcacg tatacaaaga acactccgtc ctctttccca
      181 ctgcccttaa gctcatatca gaagaagagt ggaaggatat acgtacacag tttgatgaaa
      241 ttggatacgc aggatatact ccagataagt tttctttcgt atccagtcaa gatactgcca
      301 taacaaatga ttccgtaaaa cttccgagcg gcgaactcag cctcaagcag ctgataggaa
      361 tatttgacac actccccgtc gacataactt tcattgacag ccaagacagc gtacggtact
      421 tcagtgaaag caaagacaga atttttgtca ggtccagagc cataatcggc agacacgtac
      481 agaactgcca ccccgaaaaa agtgttcata tagtaaatca gattctcagt gatttcaaat
      541 ccaacaaacg tgacagtgcc gacttttggc ttaacatagg gggaaaaacc atttacatac
      601 gttactttgc cgtacgggat actcaaggac agtaccttgg aaccctcgaa gtaacccagg
      661 atattgcccc aataaaaaag atttccggtg aaaaaagaat ctattcacac gagtaaaaat
      721 tttcttgaac cgttactttc cggtactttc ataatggaaa tcgtatactt tatcaaacat
      781 atccctgcag ctcatcctgt gcgtgatcat aaccactgaa agttttggat atgctgtgat
      841 gatgttttga agtatttcct tttccatatc ttcatcaaca gaggaaagaa cctcatcaag
      901 gagaataatt tcaggatttt tcaaaagtgc ctgggcaagg ttcagccttg ccttttctcc
      961 tcctgatatg gtttccttgc ttatcttttt gccatccttc ctatcactgt aaaacttttc
     1021 aagatgtact atcctcatta tattctcaac cttattcaca tcggtgtagg gttttccgaa
     1081 aaaaagattt tcatcaacag taccgtcaaa aactattctg tcttggttga gaagtattat
     1141 tttatccctc agatacttaa gcgagatatt tttttgctct gtaccagaaa cttttattga
     1201 tcctccgtaa tcctgcagta tccctgtcag acagttaaaa aaagtactct ttcctatacc
     1261 gctttccccg gttatcagta tcttttcacc cttacctata ctgatatccg gtatctgaac
     1321 agtttttcct ttaaatccat acgaaacacc gtttgtttga acgtcaaacc cattaagttc
     1381 ttttatgttt ccacagtctt cgttttcaag gctcaaaatg ctgtttataa gctgcatata
     1441 gggcttcatc ccgaagaagg atagtatgaa actgtacaga tctttggttg cacttacaaa
     1501 aagaggaatg aatgtataca gtattaccat gtcgccgctg cccagattga actcctttac
     1561 aaagtttatc cctaaaacta ttatgataag cggaagcaga ttggtaacta cttttaaaaa
     1621 actctgccag ctctgagtat aaactgctgt acttactatt tccttgttaa gcgaggagtg
     1681 tttgctgatg aatctgtcgt agaagaaacc gtgagagtta aaaagcttta tattctttat
     1741 gcctgtaagg gcttccacgc tgtcaccata catctctctg tacatactca tagttttagc
     1801 ctcgtgttta cgtattttta taccaaaaaa atacgtagcc agtatgataa aaggcagtgt
     1861 catgagggta accatcgccg aagaagtgtt tatcctgtat attatgacaa aatagacaac
     1921 agcctggatc aggcttatgg gaagaagaat aagtgcctgg tagaataccg atgccttgtt
     1981 agtattgttt ataatatctc cccatatctt accgggagag ttatctatga agtaagtaaa
     2041 cttggtctcc atgatttttt ttaacatcgt cagccttagt ttttttatac cgctatataa
     2101 aagcctgtga gaaaaaaaag agatcaaagt gtc
//
LOCUS       DAOSHR010000299         2127 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_51618_length_2127_cov_6.129826, whole genome shotgun sequence.
ACCESSION   DAOSHR010000299 DAOSHR010000000
VERSION     DAOSHR010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51618_length_2127_cov_6.129826"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..1203)
                     /locus_tag="PLU28_10950"
     CDS             complement(40..1203)
                     /locus_tag="PLU28_10950"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOG35618.1"
                     /translation="MKVPLFDMTRQYEKIKQEVLQTLDGIFSQGHVILGENVTMFEKE
                     IQQYLHCEHAVGVANGSDALLLAVHSLDLKPGDMVITTPYTFFATVSSITRNGALPVF
                     ADVTEKYYNIDLDMVEDILTTHPPKDRIKAFIPVHLFGKTLDLKRLEAIKKKYGISII
                     EDNAQAIGSVWHDGQTAMKFAGTAGDMGTISFFPTKNLGAYGDAGMVVTNDEKLAQRI
                     KKLRVHGAAKRYYHEEIGYNSRIDEVQAAILRIKLKHLDSWIEKRLTIAKNYQKLFQD
                     SGLDEFITYPEYFADRSHVYHQYVITLSDKKSLLRDRLAEYLQEKEIGTSVYYPRSLH
                     MQKCFDYLGCSEGQFPVSERACEKTLALPIFPELTVEEQEYVVKNIKDFFKGI"
     gene            complement(1206..1856)
                     /locus_tag="PLU28_10955"
     CDS             complement(1206..1856)
                     /locus_tag="PLU28_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HOG35619.1"
                     /translation="MFNFEEYGFFNPSPNFREMTILQMISKDPQVSQENIAKGVGIVP
                     SMVNRYLKDFEDRSFIVKTGENRRQMNYEITAEGKKRLQFLVVSFINEVSRIYFETKK
                     SFDNVLKTLERSDLSSILLYGAGVVGGIVLKVLTSEGINVAGFIDDSIAKQEEKLYGI
                     NILNPQSLEKLRYDAVIIASFKHSEEILIKAKKYNLRNIFIFKIDDTGNVSLVKEE"
BASE COUNT          595 a          430 c          364 g          738 t
ORIGIN      
        1 tggaaagtat cttttccatt aatgtcatat aattcctcct tatatccctt taaagaaatc
       61 ttttatattc tttaccacat actcttgttc ttctactgta agttccggaa aaatgggaag
      121 agcaagagtt ttttcacaag ccctttcgct gacaggaaac tgtccctcac tgcagccaag
      181 ataatcaaaa catttttgca tatgaagact cctggggtag tatacggaag ttcctatttc
      241 tttttcctgg agatactcag caagtctgtc tcttaataat gactttttat cagaaagcgt
      301 tattacatac tgatgatata catgtgatct gtctgcaaag tactccggat atgttatgaa
      361 ctcatcaagt cctgaatcct gaaagagttt ttgatagttc tttgcaatag tgagtctttt
      421 ttctatccac gaatcaagat gtttaagttt tatacgtaaa atagcagcct ggacttcatc
      481 aattctggag ttatacccga tttcttcatg gtagtatctt ttggctgccc catgtaccct
      541 gagttttttt attctctgtg caagcttttc atcattggtt acaaccattc cggcatcgcc
      601 gtatgcacca agatttttag tcgggaagaa tgaaattgtt cccatatcgc ctgctgttcc
      661 tgcaaatttc atagctgtct gtccatcatg ccatacagaa ccaatcgctt gggcgttatc
      721 ctcaattata cttataccgt acttcttttt tatagcttca agccttttca gatcaagggt
      781 tttgccgaaa agatggacag gaataaatgc ttttattctg tctttagggg gatgagtagt
      841 aagaatatct tcaaccatat ccagatctat attgtaatac ttttctgtaa cgtctgcaaa
      901 gactggcaaa gcaccgtttc tggttatgct gcttacagtt gcaaaaaaag tatatggtgt
      961 tgtaataacc atatctccag gcttcagatc aagcgaatgc acggcaagga gtagtgcatc
     1021 tgaaccgttt gcaaccccga ctgcatgttc acagtgaagg tactgctgta tttctttttc
     1081 aaacatagta acgttttcac ccaaaataac gtgtccctgg gaaaaaatcc cgtcaagagt
     1141 ctgtaaaact tcctgcttaa tcttttcgta ctgcctggtc atatcaaata aaggtacttt
     1201 catctttatt cctcctttac cagcgatacg ttccctgtat cgtctatttt aaagataaat
     1261 atatttctta gattgtactt tttagctttt ataagaattt cttctgaatg cttgaatgaa
     1321 gcaattataa cagcatcata tctgagcttt tccaagcttt gtggattaag aatatttatt
     1381 ccgtagagtt tttcttcctg cttggcaatg gagtcatcta taaagccagc tacatttatt
     1441 ccttcgctgg taagtacttt aaggactatt ccaccaacaa ctcctgctcc ataaagcagt
     1501 atagagctaa gatcggatct ttcaagagtt tttaaaacat tatcaaagga ttttttagtt
     1561 tcaaaataaa ttctggatac ttcgttaata aaagatacta caagaaattg aagtcttttt
     1621 tttccctccg cagttatctc atagttcatc tgtcttctgt tttctccggt cttaactata
     1681 aaacttctat cttcaaaatc tttgagataa cggtttacca tagaaggcac tatccccact
     1741 ccctttgcaa tattttcctg ggatacttgg ggatcttttg atatcatctg aagaattgtc
     1801 atttctctga aatttggtga tggattgaaa aaaccatact cttcaaagtt gaacacagat
     1861 acacctcttt cattcattca ctaagtgaat tatagtataa aaaaaataat ttgtcaactt
     1921 tgtttgtaag tcctcctttt aagcattcct gtaaggaatg aggtggcacc aatggtgacg
     1981 gtggatgtga ttttatgttt aaagagcaca tccctcccgg ctaacgccgt actcccctga
     2041 aggggagatt tgttaaaatc ctccttttaa ggaggtgacg gtggatgtga ttttatgttt
     2101 aaagagcaca tccctcccgg ctacgcc
//
LOCUS       DAOSHR010000300         2125 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_51691_length_2125_cov_3.357488, whole genome shotgun sequence.
ACCESSION   DAOSHR010000300 DAOSHR010000000
VERSION     DAOSHR010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51691_length_2125_cov_3.357488"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..907)
                     /locus_tag="PLU28_10960"
     CDS             complement(<1..907)
                     /locus_tag="PLU28_10960"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HOG35620.1"
                     /translation="MKPLKLTFKGINSYYDEVSIDFSALTKKGLFGIFGPTGSGKTTI
                     LDAMIIALYGQKSIPRSNADFINSKSDKAFICFEFEMDDSVYAVRRSFKRSASSIITD
                     SAVITKNSFSVAEKAKNVDAYIIETIGLEAKDFTKCVMLPQGQFNEFLTSKGRDRSEI
                     LEKIFDLQHYGSILQQKAASYFNDLKMRRSIIESKLSLYENISTQNISILKEKSNELK
                     KKKELLSSHTEQLQQETALIQSQLQDMETLRKEEKRLKELITQKEYVRSLRTTVDRAL
                     SASQVFPVLDRLSDRCCNRMPRAPRT"
     gene            complement(882..>2125)
                     /gene="sbcD"
                     /locus_tag="PLU28_10965"
     CDS             complement(882..>2125)
                     /gene="sbcD"
                     /locus_tag="PLU28_10965"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HOG35621.1"
                     /translation="NFWRRLMRILHTSDWHLGKRLEDFERFEEQESFINELSDLCDKE
                     SIDIVLLAGDVYDTYTPPSYAQELFSRAIKLITQDGKRPFIIIAGNHDSPERLSSISS
                     LAVEFGAFIFGKLEDCFSPKKYDNFEVLFTKPNFLKIQIKKETINIATLPYPGESRIN
                     MGDTAQTYLNQEEYSHIIKNISEGLCSYFDNKSINIFMSHLFVSGAFTSDSERDISLG
                     GTYVLNPSDLPAQADYIALGHLHRAQKVFSAQNACYSGSPLQYSRSESNNAKSVNIID
                     IFPSGKAQVRPILLKCIKPVEITICNGIEQAVDYYMKNSDRKFWSYLDINLKEPLSSS
                     DIQLIKSQKKDIVSICPFFEDSAQSSLTQRDIHEYSPQELFIKFYEHTEGHVPSKELT
                     ELFTKVFYEEGQEDETPKTDL"
BASE COUNT          564 a          413 c          431 g          717 t
ORIGIN      
        1 gcgttcgtgg cgcccgaggc attctgttgc agcacctgtc cgagaggcgg tcgagaacag
       61 gaaaaacctg tgatgccgat aatgctctat ccacagttgt tcttaaactg cggacatact
      121 ctttttgagt tataagctct ttaagccttt tttcttcctt tcgcaaagtt tccatatcct
      181 gcagctgtga ctgtattaaa gcagtctctt gctgtagctg ttctgtgtgg gatgaaagca
      241 gctctttctt ttttttcagt tcattgcttt tttctttaag gatactgatg ttttgagtgg
      301 agatgttttc atataacgaa agttttgatt ctataattga tctgcgcatc ttcagatcat
      361 taaagtatga agcagctttc tgttgcagaa tgctgccata atgctgcagg tcaaagattt
      421 tttcaagtat ttccgatctg tcacgtcctt tagaggtgag aaattcattg aactgtccct
      481 gtggcagcat aacgcatttg gtgaagtctt ttgcctctag acctattgtt tctatgatat
      541 atgcatcaac atttttggct ttttcggcta ctgagaatga attcttggta atcacggccg
      601 aatctgttat tatggacgag gcacttcttt tgaagcttct tcttaccgca tataccgaat
      661 catccatctc aaattcaaag cagatgaaag ctttatcgga ttttgagtta ataaagtctg
      721 cgtttgatct gggaatgctt ttctgaccgt aaagggctat tatcatagca tcaagtatag
      781 tggtcttacc gcttccggta gggccaaaaa ttccaaacaa tccttttttt gtcagagcgc
      841 tgaaatctat cgaaacttca tcataataac tgttaatgcc cttaaaggtc agttttaggg
      901 gtttcatctt cttgtccctc ctcataaaaa acttttgtga aaagctctgt aagctctttt
      961 gacggaacat gaccttcagt gtgctcatag aactttatga aaagctcttg tggactgtat
     1021 tcgtgaatat ccctttgtgt taaagaggat tgggcagaat cttcaaaaaa cggacagatg
     1081 ctcacaatgt cttttttttg gctttttata agctgaatat cagatgaaga tagaggttct
     1141 ttaagattta tgtcaagata agaccagaat ttccggtctg agtttttcat gtaataatct
     1201 acagcctgtt caataccgtt gcagatagtt atctctaccg gcttgataca tttgagtaga
     1261 attggtctga cctgagcttt tcctgaaggg aatatatcga ttatgtttac cgacttagcg
     1321 ttatttgatt cacttctgct gtactgcaag ggtgaaccgc tataacaggc attttgagct
     1381 gaaaaaacct tttgagctct gtgcaggtgt ccaagtgcta tataatctgc ttgagcagga
     1441 aggtcagagg ggttgagcac atatgtccct ccaaggctta tgtcccgttc cgaatcactt
     1501 gtgaaggcgc cgcttacaaa aagatgactc ataaagatat ttatactttt gttgtcgaaa
     1561 taactgcaca aaccttccga aatgtttttt atgatatgac tgtactcctc ctggttgaga
     1621 taagtctgag ccgtatctcc catatttatt cttgattcac caggataagg cagggttgca
     1681 atatttatag tttctttttt aatctgtatt tttaaaaaat tgggttttgt aaaaagcact
     1741 tcaaagttat cgtacttttt cggtgaaaaa caatcttcaa gctttccgaa gataaaagcg
     1801 ccaaactcaa cggcaagtga ggatatgctt gaaagtcttt ccgggctgtc gtgatttccg
     1861 gcaattatta tgaagggtct tttgccgtct tgagtaataa gctttatggc tctggaaaaa
     1921 agctcctgag cgtatgaagg cggcgtatag gtatcgtaaa cgtcacctgc aagaagaact
     1981 atatcgatac tttccttgtc gcataggtct gataactcat ttataaagct ttcctgttct
     2041 tcaaaccttt caaaatcctc gagccttttc ccaagatgcc agtccgaagt gtgaagaatc
     2101 cgcataagcc gcctccagaa attat
//
LOCUS       DAOSHR010000301         2121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_51850_length_2121_cov_2.476283, whole genome shotgun sequence.
ACCESSION   DAOSHR010000301 DAOSHR010000000
VERSION     DAOSHR010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51850_length_2121_cov_2.476283"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2121)
                     /locus_tag="PLU28_10970"
     CDS             complement(<1..>2121)
                     /locus_tag="PLU28_10970"
                     /inference="COORDINATES: protein motif:HMM:NF019217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GLUG motif-containing protein"
                     /protein_id="HOG35622.1"
                     /translation="DNLAQSWKPVGTASAPFTGSFDGNGSTVELQFIGIASSGSIEGE
                     NIGMFGVIGSTGVVKNLNFEYVFCHGKDNVGGLAGTNNGKIQNCYVKQVSYPSSVSHG
                     VEGMNNVGMFVGVNNGTVVDSKSYGNTMYAFNSAAGGFVGYNSGTIEGCAVIDGTRVS
                     THKEARAGGFVGVNTGDIIESSSDINVSDGTALDYFILGGFAGKNETTGNISYCVSSG
                     TILGYSAVQSAGFVLENKGTIDRSYSNMKVKASTGKIYGFGPGTTGITNCYWICYDNN
                     QYQAGSATGLTEAAAKKTSSYLNWNFGDKWVIDTEIPILARTLPVKYDEGDGSLTNPF
                     VIKNFEQLNAMRWRLSKHYILAQDIRINDTWYPVGSYLNEEIRYFTGSFDGNNKTIDN
                     LRIGWMTKGSGMFGYVKSAVIKNIKFTNANVNSGESAGLLVGFADGVQAEGSTPSNFT
                     KIDNIQIMSGIITGAKEVGGLIGRGNKTEITNVSVKANVSGNEYVGGVAGYLEIARAK
                     DILYEGNTTGTKACGGIAGKFIVDSIDGTYIYTVQNIASKGKVSGEYNVGGLIGQAWG
                     KYENISSESEISGKELWLPMETGSGIGGLFGSSFGSDVKNSFFKGQITMQKGFCGGLV
                     GYAEGGNITDCYLQGSINYIYADTTSFAGGILGDGQANISGCYSCGSISGSNYSAGLV
                     GRFRNTLGCTIKDSYSRGSAYAGL"
BASE COUNT          580 a          520 c          335 g          686 t
ORIGIN      
        1 ataagaccgg catacgcact tccacgagag taactgtctt ttatcgtaca gccaagagta
       61 tttctgaagc gtcccaccag acctgcagag taatttgatc ctgagattga accacaagag
      121 taacaaccgg atatgtttgc ctgtccgtca cccaaaattc ctcccgcaaa agacgtcgta
      181 tctgcatata tatagttaat tgatccttgt aagtaacaat ccgttatatt tccaccttca
      241 gcataaccta ccagaccgcc gcaaaaacct ttctgcatag ttatctgccc tttgaagaat
      301 gaatttttaa catctgagcc aaaggaagaa ccaaaaagcc ctcctattcc acttccggtt
      361 tccataggca gccataattc ttttccactt atctctgatt ctgacgatat attttcatac
      421 tttccccagg cttgcccgat taatccgcct acattatact ctccagagac ttttcctttt
      481 gaagcaatat tctgaaccgt atatatgtaa gtaccgtcaa tagagtctac tatgaatttt
      541 cccgctatgc ctccgcatgc tttggttcct gttgtatttc cctcatacaa aatatcttta
      601 gcacgtgcaa tttccagata tcctgccacg ccgcccacat attcatttcc gcttacattg
      661 gcctttacgg aaacattagt aatctctgtc ttattacctc tgccgatcaa tccgccgact
      721 tctttggcgc ctgttattat accgctcata atctggatgt tgtctatctt tgtaaagttg
      781 cttggagttg aaccttctgc ctgtactccg tctgcaaagc caacgagcag acctgcgctt
      841 tcacctgagt ttacattggc attagtaaat ttaatattct ttataactgc actcttgaca
      901 tatccgaaca ttcctgatcc tttggtcatc catccgatac gcagattatc tatggtttta
      961 ttatttccat caaaggagcc tgtaaagtat ctgatctctt cattcagata cgagcctaca
     1021 ggataccatg tatcgtttat ccttatatct tgtgccagaa tataatgttt tgaaagtctc
     1081 catctcattg cattaagctg ttcaaagttt ttaatgacaa atggatttgt caacgaaccg
     1141 tcaccctcat catactttac cggaagggtt cttgcaagga taggaatttc agtatcaata
     1201 acccatttat ctccaaaatt ccagtttagg tacgatgatg ttttttttgc agcagcttca
     1261 gtaagtccgg tcgctgaacc tgcctgatat tgattattat cataacatat ccagtaacaa
     1321 ttggttattc ctgtagttcc tggtccgaaa ccataaattt ttccggtgga agctttaact
     1381 ttcatatttg aatatgatct gtctatggtt cctttatttt caagtacaaa ccctgccgac
     1441 tgcacagcgc tatatccaag aattgttcca gaagatacac agtatgatat attccctgtg
     1501 gtttcatttt ttccggcaaa tccgcctaaa atgaagtaat ctaaagccgt cccatcactt
     1561 acatttatgt cacttgaaga ttcaattata tcgccagtgt ttactcctac aaagccgcct
     1621 gcacgagcct ctttatgagt agaaactctt gttccgtcaa taactgcaca gccttctatg
     1681 gtaccgctgt tataacctac gaaaccacct gctgcactgt taaacgcata catagtattt
     1741 ccgtagcttt tagagtccac tactgttcca ttgttaaccc caacaaacat tcctacattg
     1801 ttcatccctt ctactccatg tgagacactt gaaggataag aaacttgttt aacataacag
     1861 ttctgaattt ttccattgtt tgttcctgca agtcctccta cgttatcttt tccatgacaa
     1921 aacacatatt caaaattaag gtttttgact actccagtgg atcctataac accgaacata
     1981 cctatatttt ctccttcaat agagccggaa gaagcaatac cgataaactg tagctctact
     2041 gtagagccgt tgccgtcaaa acttccggta aaaggagctg aagcagttcc tacaggtttc
     2101 caactctgtg caaggttatc a
//
LOCUS       DAOSHR010000302         2113 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_52132_length_2113_cov_4.416424, whole genome shotgun sequence.
ACCESSION   DAOSHR010000302 DAOSHR010000000
VERSION     DAOSHR010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52132_length_2113_cov_4.416424"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="PLU28_10975"
     CDS             complement(<1..598)
                     /locus_tag="PLU28_10975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35623.1"
                     /translation="MNKEELKRTLKKFFKNSSAMLGLILLIFFVIIAILAPVLAPARV
                     PEDLDLDIAKNIMTEYSAEKRPEIINAYDSFITTLFIFYTASKEDIQSLEPLIKNYEK
                     DHSEIVALRDKVKVLCDDYILDSSYYNRLTLTELEKLVSKYENKEVSKEEFLKVLEKN
                     YVKTFAIDASVLAKIDKDEKATLRFIRSQIKTNISKAVK"
     gene            complement(609..1646)
                     /locus_tag="PLU28_10980"
     CDS             complement(609..1646)
                     /locus_tag="PLU28_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG35624.1"
                     /translation="MTAYIIRRLLVLPIMLLGVTIIVFSMMQILGPDKLLSAYVDPNK
                     AEKLSIKELEMLKERYGLNDPLVIRYGKWIGNTLQGDLGWSQVGRAPVLDSIVSRFPY
                     TIELAIYSVIPIIGIGIWLGVLAAVKHNSFIDQFVRIFAIVGWSFPDYVFALLIILIF
                     YSWLGWFPPGILSVQMEQVVNSAGYHQFTRLLTLDGLLNGRLDVFWDALRHIIGPILT
                     LSYIQWAYLLRITRSSMLEVLRKDYVRTARAKGLDEKVVINKHAKRNALIPVVTVAGQ
                     LLIGLLMGVIIVEAVFNRPGLGSFVAQSASQLDYASVMGALLFSAAIMIVGNLIIDIS
                     YAFIDPRIRLE"
     gene            complement(1728..>2113)
                     /locus_tag="PLU28_10985"
     CDS             complement(1728..>2113)
                     /locus_tag="PLU28_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOG35625.1"
                     /translation="RSELPLFMMGWGADFPDPHNFVFTYYHSKGDLSSFYGENFINYA
                     TTPNAELDGLSLDQIVEKAGLETDTKTRVALYNKIQNFAINHALGMPVYQPIALKGMR
                     TWIKGWYTNPQKGSDDYFYTLSKAE"
BASE COUNT          641 a          426 c          385 g          661 t
ORIGIN      
        1 cttttactgc ttttgaaata ttagttttta tctgtgaacg aataaacctg agagtggctt
       61 tttcatcttt atcgattttt gcaagtacgg aagcatcaat ggcaaaagtt ttcacataat
      121 tcttttcaag aactttaaga aattcttcct tcgatacttc tttattttca tactttgaaa
      181 caagtttttc aagctcggta agcgtaagcc tgttatagta agaactgtca agtatgtaat
      241 catcacaaag aacttttacc ttatcacgta aagcaacaat ctctgaatga tctttctcat
      301 aattttttat taggggttcc aaggattgaa tatcttcttt tgaagcagta tagaaaataa
      361 aaagggttgt aatgaatgag tcataagcat taattatctc cggccttttt tcagcagagt
      421 actctgtcat aatgtttttt gcaatatcca aatcaagatc ttccggtact cttgccggtg
      481 caaggactgg agcaagaatc gctataatca caaaaaatat aagtaaaata agtccgagca
      541 tagcagagga atttttaaaa aacttcttaa gtgttctttt gagttcttct ttattcatta
      601 tcaacacctc attccagtct gattcgcgga tctatgaaag catacgatat atctataata
      661 agatttccaa caatcattat tgctgctgaa aacagaagtg cacccattac agaagcgtaa
      721 tccagttggg atgcagattg agcgacgaaa ctgcctaatc cgggtctgtt gaatactgct
      781 tctactatta ttacccccat aagaagtccg ataagaagct gcccggctac tgtgacaacc
      841 ggtataagag catttctctt tgcatgtttg tttattacta ctttctcatc caaacccttt
      901 gctcttgctg ttcttacgta atcctttcgt aaaacctcga gcatagatga tctggttatt
      961 ctaagcaaat atgcccactg tatataggaa agagttagta taggacctat aatatgccgt
     1021 aaggcatccc aaaaaacgtc aagccttcca ttgagaagac cgtccaatgt aagtaacctt
     1081 gtaaactggt gataacctgc agagttaaca acctgttcca tctgcacgct taagattccg
     1141 ggagggaacc agccaagcca actgtaaaag ataagaatta taagcagggc aaaaacataa
     1201 tccggaaatg accatcccac aatagcaaag attcgtacaa actggtcgat gaaagagttg
     1261 tgctttacag ctgctagcac tcctagccat attcctatac ctattatagg gatgactgag
     1321 tatattgcca gttcaattgt ataaggaaac cttgatacta tggaatccag aacaggagct
     1381 cttcctacct gtgaccaccc gagatctccc tgaagagtat ttcctatcca ttttccgtat
     1441 cgtataacaa gaggatcatt gagtccatat ctttccttta gcatttccag ttccttaata
     1501 ctgagctttt cagctttatt tggatcaaca tatgctgata acagtttatc cggtccgagt
     1561 atctgcatca ttgagaaaac aattatggtt accccaagta gcattatcgg aaggacaagc
     1621 aatcgcctga tgatatatgc ggtcaaaaga attcacctcc ggttaataat tgattttaca
     1681 taaacatgag ggggaagatt acgcttcccc cccattttga ttaaatttta ttcagctttg
     1741 gacagagtat agaaatagtc gtctgaacct ttctgcgggt tagtatacca tcccttaatc
     1801 catgttctca tgccttttaa tgctatcggc tgatacacag gcataccaag agcgtgattt
     1861 attgcaaagt tctggatttt attgtaaaga gctactcttg ttttggtgtc tgtttcaaga
     1921 ccggcctttt caactatctg atctagtgat aaaccgtcca gctcagcatt aggagtcgtt
     1981 gcatagttaa tgaagttctc tccgtagaag cttgacaggt cacctttgga gtgatagtaa
     2041 gtgaatacaa agttgtgtgg atctgggaag tcagcacccc aacccatcat aaagagagga
     2101 agttctgatc tct
//
LOCUS       DAOSHR010000303         2103 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_52479_length_2103_cov_4.091797, whole genome shotgun sequence.
ACCESSION   DAOSHR010000303 DAOSHR010000000
VERSION     DAOSHR010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52479_length_2103_cov_4.091797"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1084
                     /locus_tag="PLU28_10990"
     CDS             <1..1084
                     /locus_tag="PLU28_10990"
                     /inference="COORDINATES: protein motif:HMM:NF015022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HOG35626.1"
                     /translation="ASKKRKRYIQMNKDKTYNIVMAGGGTGGHYYPALAFVNELSKKY
                     QTRIIYFATKGRIEEKYLPVDIPWAKVFTVELTGLKRPVYSFENFSVIKKMYSEKSRI
                     KKIIKDFSPDFGFLTGGYISAPVGLNLRDLKVPFFLHEQNSIICFTNRRLSKYAQKVF
                     VSFQETVLSEKFLLTGNPVRLPKNSISREYLCTMGIKDTGKRTVLVFGGSLSSEKIDE
                     LMLKVYPLDTQTNFIHVTRQPDLFEQFKNVFTREYIPDIYNLLAICDGVICRAGATSI
                     AEIKYFGLKALLVPWRGAAEDHQFRNALSLEKLGLADIIAEDEKDISKITGIIQRMPN
                     KGSSFVWNVHEDTSAKKIIDNIEQLI"
     gene            1102..>2103
                     /locus_tag="PLU28_10995"
     CDS             1102..>2103
                     /locus_tag="PLU28_10995"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HOG35627.1"
                     /translation="MKYFFSGIGGIGMSSLALYANYCGHKVYGSNNEKNERVDYLRSK
                     GVEISIGHSKNLPDIDLLIRSTAIRNNNPEVVAAESKNIPTAFRMEYLSNILKSTWSI
                     GITGTDGKTTTTAMMSKIFKTAGKDPTVFLGGIHSCLEDGNFRMGSGQIIAEVDESDG
                     YIKDAQTDVSVVTNLRPDHLEHYANRFENLEYAVKRYSERSRDIIFLNGDDPILSQWA
                     EEFPNSVTMGTSEKNDYFIKNRAMKDHMQSFDLYYKNKFVLNINLRLPGLHYAMDALV
                     ASSVALEYGIKTEDIIDGLYTYDTVDRRFNILYSNTDLFIIDDYAHTPDEIYSTIQAT
                     "
BASE COUNT          700 a          408 c          432 g          563 t
ORIGIN      
        1 agcatcaaaa aaaaggaaga ggtacattca gatgaacaaa gacaagacat ataacatagt
       61 aatggcaggc ggaggcacag gaggacatta ctacccggct ttagctttcg ttaacgagct
      121 ttcaaaaaaa taccagacaa gaattattta ctttgctacc aaaggacgta tagaagagaa
      181 gtatctgcct gttgatattc catgggcaaa ggtttttact gtcgagctta caggtcttaa
      241 gagacctgtt tacagtttcg agaatttttc tgttataaaa aaaatgtata gtgaaaaatc
      301 acgtataaaa aagataataa aggatttttc cccggatttc ggttttctta ctggaggtta
      361 catcagtgct cctgtaggac ttaatctcag ggatcttaaa gtaccttttt ttctgcacga
      421 gcaaaactct ataatatgtt ttacaaaccg aaggctttca aagtatgcac aaaaagtctt
      481 tgtatctttc caggaaacag ttctgagcga aaaatttctg cttaccggaa atcctgtaag
      541 gcttcctaaa aacagcataa gcagagagta tctttgtaca atggggataa aagatacggg
      601 caaaagaacg gttcttgttt tcggaggaag cctgagttcc gaaaaaatag acgagcttat
      661 gcttaaggtt taccctctgg acactcagac caactttatc catgtcacaa ggcagcctga
      721 tctttttgag cagtttaaga atgtttttac ccgtgaatat atacccgata tatacaatct
      781 tcttgccata tgtgacggtg taatctgcag agcaggggca acaagtattg ctgaaataaa
      841 gtactttggt cttaaagcac ttcttgtgcc atggcgcggt gcggccgaag atcatcagtt
      901 cagaaatgcc ctttcgcttg agaaacttgg actggccgat ataatagcgg aagatgaaaa
      961 agatatttcc aagataaccg gaataataca gaggatgccc aataaaggtt cttcttttgt
     1021 ctggaatgtt catgaagaca catcagctaa aaaaattatt gataatatcg aacagttaat
     1081 ataattttag gaggagtaat aatgaagtac tttttttcag gcattggcgg tataggcatg
     1141 agcagccttg cactgtatgc aaactactgc ggccataaag tttacggttc aaacaacgaa
     1201 aaaaacgaac gtgttgatta cctgaggtcc aaaggtgtag agataagcat tggacacagt
     1261 aaaaatcttc ctgacataga tcttcttata aggtcaaccg ccatacgcaa caacaacccg
     1321 gaggttgtcg ctgcggaatc aaaaaacatc cctacagctt tcaggatgga gtatctgagc
     1381 aacatactca aatcaacctg gagtatagga ataactggta cagacggaaa aacaaccact
     1441 acagccatga tgtccaagat cttcaaaaca gccggaaaag accctactgt ttttcttggc
     1501 ggcatacaca gttgccttga ggacggcaac ttccggatgg gaagcgggca gataatagct
     1561 gaagtggatg agagcgacgg ttatattaaa gatgcccaga ccgatgtgtc agttgtgacc
     1621 aacctcagac ctgatcatct tgaacattac gctaaccgct ttgaaaatct tgaatacgca
     1681 gttaaaagat actccgaaag atcaagagac ataatctttc taaatggaga cgaccctatc
     1741 ctttcacagt gggctgagga gtttcccaac tccgttacta tgggaacttc cgaaaaaaat
     1801 gattatttca taaagaacag agccatgaaa gaccatatgc agtcatttga tctttactac
     1861 aaaaacaagt ttgttctgaa tataaatcta agacttccgg gactccatta tgctatggat
     1921 gctttggtag cttcctcggt agctttggaa tacggtataa aaacagagga tataatagac
     1981 ggtctttaca cttacgatac cgtagacaga agatttaata ttctttattc caacacagac
     2041 ctttttataa ttgacgacta tgcgcacact ccggacgaga tatactccac tatacaggcc
     2101 aca
//
LOCUS       DAOSHR010000304         2098 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_52652_length_2098_cov_4.307881, whole genome shotgun sequence.
ACCESSION   DAOSHR010000304 DAOSHR010000000
VERSION     DAOSHR010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52652_length_2098_cov_4.307881"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="PLU28_11000"
     CDS             <1..243
                     /locus_tag="PLU28_11000"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOG35628.1"
                     /translation="NDLGIHDMNGNVMEWCSDFYAPYFGNTLKNPIQTTGKRKVVRGG
                     SYNYLSSLSRNSKRMSFDPEFKSGYLGFRVVRSILP"
     gene            345..872
                     /locus_tag="PLU28_11005"
     CDS             345..872
                     /locus_tag="PLU28_11005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOG35629.1"
                     /translation="MTKKINLKRITRTPIWIAAGVAFFSFFLHGMGNPKIGQMFLPMH
                     FVALLAGLVDGPAIGFMAGLLMPVLSTLISGLPPYPMFLFMMAEISLYGFFSGLLRKT
                     NVFLNLLISILIGRLAYAISYYALGSVLSIKMTPVLSLLLSFLNGLPGIAIQLIFIPL
                     IYFKLLKISATQKES"
     gene            874..1620
                     /locus_tag="PLU28_11010"
     CDS             874..1620
                     /locus_tag="PLU28_11010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HOG35630.1"
                     /translation="MNIHFFGGIFRDTLIYSNEPHNSEVYDLFGGSAFNMASIAINFF
                     ENVFLHSSIGTDWSVSFKKENQRIRTDFLAVSETFRTARFISVNNEVLAVDRKILDQY
                     SIDELSNIDSESDVAVLTTELSKKNIQTVLSKKWFKLFIDAGPRPYLIDIKDIEDAFV
                     LGNRKEASVVNCDIIKCGHNGAIYKNTIFSSDNIIYKYPIGCGDIFDAIFICAVLKGI
                     PYDQAVRNAVNVASICSSVPEKNKLLPDIL"
     gene            1634..2011
                     /locus_tag="PLU28_11015"
     CDS             1634..2011
                     /locus_tag="PLU28_11015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35631.1"
                     /translation="MKKYFLLLVSLVLISILMISCSGSFSYSGSFLEGRTGMQAELSG
                     SINKSYAYELKASSKLEISITTSGRLKVQIYDPNGKLVYERAVIDESISEAVDTLEND
                     GKYTFHFTSSDLSSASVKFEFVR"
BASE COUNT          656 a          360 c          377 g          705 t
ORIGIN      
        1 aatgatctgg ggattcacga tatgaacgga aacgttatgg agtggtgttc tgatttttat
       61 gcaccttatt ttggaaatac tcttaaaaat cctattcaga caaccggaaa aaggaaggta
      121 gtacgcggag gaagctataa ttatctttcg tcactttcaa gaaacagtaa aagaatgtct
      181 tttgatcctg agtttaaatc cggttatctc gggtttagag tagtacgctc catcctgcca
      241 taaaataact tgacatagga tatctaaatt aatataattg aactgcattt tcttgagcat
      301 aatcatttga agctctttta tttagaactt atcaggaggg actgatgact aagaaaataa
      361 acctcaaaag aatcacacgt actcctatat ggattgcagc aggtgtagcc tttttttcat
      421 ttttccttca tggaatggga aatccaaaaa ttggccagat gtttcttccc atgcattttg
      481 tggccttact tgcaggatta gtggatggtc cggcaatagg gtttatggct ggtcttctta
      541 tgccggtgct cagtacactt atttcaggtc ttcctcctta ccctatgttc ctgtttatga
      601 tggcggagat aagcctatat ggattttttt cgggactgct taggaaaacc aatgtttttt
      661 taaatctatt aatttcaata cttatcggca gattggcata tgctatttct tactatgctt
      721 taggcagtgt gctatcgata aaaatgactc ctgtattaag ccttcttctg tcatttctta
      781 acggtttacc gggaattgca attcaactta tttttattcc gttgatttac tttaaattac
      841 ttaagatatc agcaactcag aaggagtctt aatttgaaca ttcatttttt cggaggtata
      901 ttcagagaca ctctcatcta ttcaaatgaa ccccacaatt ctgaagtata tgacttattt
      961 ggaggatccg ccttcaatat ggcatctata gccataaatt tttttgaaaa tgtatttcta
     1021 cattcttcaa taggtactga ctggtctgtt tctttcaaaa aagaaaatca aagaataaga
     1081 acagactttt tagcagtttc agaaacattt agaaccgcaa gattcatatc tgtaaataat
     1141 gaggtactgg ctgttgatcg gaaaattctt gatcagtact ctattgatga actttccaac
     1201 atagattctg aaagtgatgt agcagtttta accacagagc tttctaaaaa aaacattcag
     1261 accgtgcttt caaaaaaatg gttcaagctt tttatcgatg ccggtccaag accatacttg
     1321 atagatatca aagatattga agatgctttt gtattaggaa acagaaagga agcttcagta
     1381 gtcaactgtg acattataaa gtgcggacat aacggagcta tttataagaa tacgattttt
     1441 tcatccgata atattatcta taagtaccct ataggctgcg gcgatatatt tgatgctatt
     1501 tttatctgtg ctgtacttaa aggtattcca tacgatcagg ctgtgcgtaa tgcggtaaat
     1561 gttgcttcaa tttgttcatc agttccggaa aaaaataaac ttctgccgga tatattataa
     1621 aaggaggcca ttcatgaaaa aatatttttt gttactcgtt tcactggttt taatatccat
     1681 actcatgata tcctgttccg gttccttttc ttacagcggt tcctttcttg aaggaagaac
     1741 cggcatgcag gcggaactct caggcagtat caacaaatct tacgcttatg agctaaaagc
     1801 atcctcaaaa cttgaaatat ctataactac ttcaggaaga ttgaaagtcc agatatatga
     1861 tccaaatgga aaactcgtat atgagagggc tgtaatcgat gaaagtatat ctgaagcagt
     1921 tgatacactt gaaaatgatg ggaaatacac ttttcatttt acttcttcgg atctatctag
     1981 tgcatctgta aagtttgaat ttgtaagata atcataataa taaaactgct aactgtaatt
     2041 ttcaaaaaca taaaatttaa ctgtcaaaaa gtataattcc ctcgtgcttt aagcagga
//
LOCUS       DAOSHR010000305         2095 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_52773_length_2095_cov_3.580392, whole genome shotgun sequence.
ACCESSION   DAOSHR010000305 DAOSHR010000000
VERSION     DAOSHR010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52773_length_2095_cov_3.580392"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2006
                     /locus_tag="PLU28_11020"
     CDS             <1..2006
                     /locus_tag="PLU28_11020"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HOG35632.1"
                     /translation="LVVGVIASKPLYSLFSDYDQSSYKDEVFRVYSKILIDTPLTQTL
                     KLSSEISREEVSMLMVNAFKLSKIQPMMIKTDKKKTFSYTDPLGVIDESFSIPSAQDI
                     VESYYLAYIEGLIKIRAVQVSGERFNPTEPLSHVDFFEMLGKIIFGVDEKHDYLKEIA
                     GMGFTAEEVSSKTNVTWEKALSYLDRILSKSDFKVISVLTTADIHGNLQQYIPSGSTY
                     NIGGLSRMSKTVSDIRASQNNLLLLDVGDAPYNTNVANMFEGRPVISIMNLMKYDAMA
                     IGNHDFDFPFDNMRNNSKIASFPFLSANTLYEGKNPDFLKPYVIKDVDGVKIGIIGLT
                     DHDSAWYTHPNNVKGISFINHFQAAEKYVNELKGKVDIIISLAHLHGDNSVLPQKVTG
                     IDFELAGGQDIVAYPQKINDTWVITSGKHSELVSRLNINMIGNKIIGFNFAHIFLTEN
                     LPQDPHINNIIAMYSDNLNKSMSQVVGLTQVDLDGERGTVRLKESNLGNLIADSLRDF
                     GGADIALQNGGGIRASIKKGQITLKDIYTVLPFDNAVVVFKASGKVIWDTIEHSISQY
                     PSAAGQFLQVSGMTYKFDASKEPYKRLLEIKINNEPIDLEKIYTVVANDFMTGGGDKY
                     TMLRDNTQITIKTREYLRDVLKLYLMKVREVAPILEGRIEIVK"
BASE COUNT          713 a          355 c          430 g          597 t
ORIGIN      
        1 ttttggtagt aggagtcatt gcctcaaaac ctttatattc gcttttcagt gattatgacc
       61 agagcagtta taaggatgag gttttcagag tttattcaaa gatccttatc gatacgcctt
      121 tgacacagac gttgaaactt tcttcagaga taagcagaga agaagtttcg atgcttatgg
      181 tgaatgcgtt caagctttca aaaatacagc ctatgatgat aaagactgat aaaaagaaaa
      241 cattctctta taccgatcct ttaggagtta tagatgaaag cttcagtatt ccgtctgcac
      301 aggatatagt tgaaagttat tatcttgcct atatagaagg ccttataaaa ataagagccg
      361 ttcaggtcag tggggaaagg ttcaatccta ccgaacctct tagtcatgtt gatttttttg
      421 aaatgctggg gaaaataatc ttcggggtag atgaaaaaca tgattactta aaagaaatag
      481 ccggtatggg ctttactgca gaggaagtat cttctaaaac taatgtaacc tgggaaaagg
      541 cgctgtcata tttagacagg atactttcaa agtctgattt taaagtaatt tccgttctta
      601 cgacagcaga tatacacgga aacctccaac agtacattcc atcaggttct acgtacaaca
      661 tcgggggcct ttcacgtatg agcaaaacag tttcagatat cagagcatca cagaataatc
      721 ttcttctgct tgatgtaggg gacgctccat ataacacaaa tgttgcaaat atgtttgaag
      781 ggcggcctgt cataagcata atgaatctta tgaaatacga tgcaatggca ataggaaacc
      841 acgattttga ttttcctttt gataatatgc gaaataactc aaagatagca tctttccctt
      901 ttctttctgc caatactctc tatgaaggga aaaacccgga ttttcttaag ccttatgtaa
      961 taaaagatgt agacggggta aaaataggaa taattgggct taccgatcat gacagcgcat
     1021 ggtacactca tccgaacaat gtaaagggaa taagctttat aaaccatttt caagctgcag
     1081 aaaaatatgt caatgaatta aaagggaaag ttgatataat aatttctctt gctcacctcc
     1141 acggagataa ttcggttctt cctcaaaaag taaccggaat tgattttgag cttgccggtg
     1201 ggcaggatat agttgcttat ccacaaaaaa tcaacgatac atgggtaata acctctggta
     1261 agcactctga actcgtttca agactcaaca taaacatgat tgggaataaa ataataggtt
     1321 ttaattttgc gcatatattt ttgactgaga atctccccca agatcctcat ataaataata
     1381 ttatcgcaat gtactcagat aatctgaata aaagcatgtc acaggtagta ggtcttacac
     1441 aggttgatct tgacggagaa agaggaactg tgagattaaa agaatcgaat ctgggaaatc
     1501 ttatagctga cagtctcaga gatttcggcg gcgctgatat tgccttacag aacggtggcg
     1561 gaataagggc aagtatcaaa aaaggtcaga ttacgcttaa ggatatatat acagttcttc
     1621 cttttgataa tgctgtggtc gtatttaaag ccagcggaaa agtaatatgg gacacaatag
     1681 aacactcaat atcgcagtat ccttccgcag ctggtcagtt cctccaggta tcaggcatga
     1741 cctataagtt tgatgcttca aaagaaccat acaaaagact tctggaaata aagataaaca
     1801 atgagccgat cgatcttgaa aaaatctata ctgtagttgc aaacgatttt atgacaggag
     1861 gcggcgataa atacactatg ctcagagata atactcaaat aacaattaag acacgagagt
     1921 acctgcgtga tgtgctgaag ctgtatctga tgaaagttcg tgaagtagct ccgattttag
     1981 aaggaagaat agagattgta aagtagataa gaaacgggga taatttcccc gtttcttaaa
     2041 tttttggtat aataattgtt aaggatagaa aaaggagggg gaaaatgtac aactt
//
LOCUS       DAOSHR010000306         2094 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_52802_length_2094_cov_3.379598, whole genome shotgun sequence.
ACCESSION   DAOSHR010000306 DAOSHR010000000
VERSION     DAOSHR010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52802_length_2094_cov_3.379598"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1585
                     /locus_tag="PLU28_11025"
     CDS             <1..1585
                     /locus_tag="PLU28_11025"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOG35633.1"
                     /translation="IYNNNLKSIQYISALQNSLLEHSRDTFLVLLTDDKQVLNTIKAE
                     TANFFIQEDAYLQSYMNSVKNVISDRELENIKVVLNDYKNISSKLFSMLESSGKEEIL
                     MYIQNTAGPKVMQIEKLLQSLIDKNTVNADDVYKSSAGMADRTAEIFLIIIIFAISIS
                     GVLSILVTISIISPIKKLQKNIELFGKGDFTVAFAQPGRNEISSMSVSLQNMADSLKK
                     IFLGVRDTTKLVDTSSDELSAIAEQTKEGLENTDHAVEVFDKNVQGTTASIQEVTSSV
                     QEVTAALMNITKLHQELLSKAEDVSVRSSDGTVGIEKIVELANNSVQSSIETAQTVRS
                     LAEKSEKIGEIVQNINSIAEQTNLLALNAAIEAARAGEAGKGFAVVADEIRKLAEESK
                     KTTSNISDILNDIKSGVVQAQNATEKTVENGKEVDKKAKNVAENFVGINKSVNEIKNS
                     FQDLSASSQEVSASAQEISASMDTANVSMTKIAEEIQNINQTLDSQVQSANSVSDMSE
                     NLSKLSDSLLNKIENFKVD"
     gene            1726..>2094
                     /locus_tag="PLU28_11030"
     CDS             1726..>2094
                     /locus_tag="PLU28_11030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35634.1"
                     /translation="MRHYKKLAAALFISFFTTLAFSETTVFFQWKEVESCFYTQQADC
                     VYVDIQALCSRFDYKTELSQDQIIRIYNNHTDILISFYNCSYTVMESPAVFFNKYDFI
                     IKNGTVFMNAELICSILDLTF"
BASE COUNT          724 a          373 c          406 g          591 t
ORIGIN      
        1 gatttataat aacaatctta aatctataca gtatatatcg gctttacaga attcactact
       61 tgaacacagc agggatactt ttctggtgct cctgacagat gataagcagg tacttaatac
      121 tataaaagca gaaacagcaa actttttcat ccaggaggat gcttatcttc agagctatat
      181 gaattcagtt aaaaacgtta tatccgacag ggagcttgaa aacataaaag ttgtcctgaa
      241 tgactataaa aacatttctt caaaactttt cagcatgctt gaaagctccg gtaaggaaga
      301 aatccttatg tatattcaaa acaccgccgg tccaaaagta atgcagattg aaaagcttct
      361 gcagtcgctt atcgataaaa acaccgttaa tgctgatgat gtttataaaa gcagtgcagg
      421 aatggcagac aggacagcgg aaatatttct tataatcatt atttttgcca tttcaatatc
      481 aggcgtactg agcatacttg ttactatttc cataatctct cctataaaaa agcttcagaa
      541 aaatatagag ctttttggaa aaggggattt tacggtagcg tttgcccagc caggcagaaa
      601 cgaaatatct tccatgtcgg tttcgcttca gaatatggct gattctctga aaaaaatatt
      661 tttgggagtc agagatacca ccaagctcgt ggatacttcc tctgatgaac tgtctgcaat
      721 agcagaacag acaaaagaag gtcttgaaaa tacagatcat gctgttgaag tttttgataa
      781 aaacgttcag ggaacaaccg cttcaataca ggaggttact tcatcagtac aggaagtaac
      841 ggctgctctt atgaatatta ctaaactgca tcaggagctt ctttcaaagg cggaagatgt
      901 ttcggtacgt tcaagtgacg gaacggtagg aatagagaaa atagttgaac tcgccaataa
      961 ttctgttcag agcagcatag aaactgctca gactgtaaga agccttgcag aaaaatcaga
     1021 aaagatagga gaaatagtcc agaatataaa ctctattgcc gaacagacca accttcttgc
     1081 acttaacgcc gcaatcgaag ccgccagagc aggtgaggca ggaaagggat ttgccgtggt
     1141 tgccgatgag ataagaaagc tggctgaaga gagcaaaaag accacatcaa acatttctga
     1201 catcctcaat gatataaaat cgggcgtagt acaggcacag aatgctactg aaaagactgt
     1261 tgaaaacgga aaagaagtgg acaaaaaagc aaaaaatgtt gcagagaact ttgtcgggat
     1321 aaacaagagc gtcaatgaaa ttaaaaattc attccaggat ctttcggcaa gctcccaaga
     1381 ggtaagtgct tcggcacagg agatatctgc ttcaatggat acggccaatg tatcaatgac
     1441 taaaattgca gaggaaatcc agaatataaa ccaaacactg gattcccaag tccagagcgc
     1501 aaacagcgtt agcgatatga gcgaaaattt aagtaagctt tctgattcac ttttaaacaa
     1561 aatagaaaat tttaaggtcg actgatggta agataaagga aaagcactat gaagaaaaac
     1621 ttcatagtgc ttttcttatt ttctgccata aaaacagata tctatgataa aattattcat
     1681 ataataataa tctttctctg ttatttcatt tggcgggctg atattttgag acattataaa
     1741 aaacttgcag cggcattatt tataagtttt tttaccactc ttgctttttc cgaaacaact
     1801 gttttttttc agtggaagga agtcgaatcc tgtttttata ctcaacaggc cgattgtgtt
     1861 tatgttgata ttcaggcgct ctgttcgaga ttcgactata agacagagct ttcccaagat
     1921 cagattatcc gcatatataa taatcatacg gatatactga tttcatttta taattgctct
     1981 tatactgtaa tggaaagccc agcagtattc tttaataagt atgattttat cataaaaaac
     2041 ggaactgtct ttatgaatgc cgagcttatc tgctcaattc ttgatctaac tttt
//
LOCUS       DAOSHR010000307         2079 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_53402_length_2079_cov_3.728755, whole genome shotgun sequence.
ACCESSION   DAOSHR010000307 DAOSHR010000000
VERSION     DAOSHR010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53402_length_2079_cov_3.728755"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2020)
                     /locus_tag="PLU28_11035"
     CDS             complement(<1..2020)
                     /locus_tag="PLU28_11035"
                     /inference="COORDINATES: protein motif:HMM:NF028476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 36"
                     /protein_id="HOG35635.1"
                     /translation="MQKLFESKYGYFDKNGAEYVITDPQTPRPWVNVISNGDYSIIVS
                     QNGSGYSFRSNAGENRITRSYQDLIKDNWGKYYYIRDLEDKEYWSVGRKPVMHAFDFY
                     EVRHGIGYTSILQRVKDIETEILFFVVPNYPVEIARIRVKNMGSTIRKLDLTSYTEWV
                     LGSFPDEHREFHKIFVDSKFTKNAILAEKLICAFPDEKGRHNNRSWDQTAFHAVSEEV
                     KSYDGDKESFIGMYRDESKPQAMEKDLLGKKTGRFTDPIAALQVEIKLQAGEEKTVVF
                     TTGTAYKGKEDPAELIEKFTSVQACEREFKNLSVFWSEFLDCEIKTPDKAMDIMTNIW
                     LKYQAISGRIWAKSGYYQVSGGYGFRDQLQDSLIFLTCKPELTKKQLLMHAAHQFRDG
                     DVLHWWLTIGNWGPRTKCSDDLLWLVFIAYYYIQETGDFSILDETVPYYDGGQDTFYI
                     HCKKAVEKVMSRFSPRGVPLMGDNDWNDGLSAVGTDWKGESFWMSEFFYMVLGQFMDF
                     GSYVKDNAFLKKAEDVMQTLKESFEKYGWDGKWYLQATTDGFEKIGSNENEEGKIFLN
                     PQLWSLISSIGDSQRAITAMENVTEHLLKDYGALLLAPAYTAPRTDIGYITRYAPGLR
                     ENGGVYTHAATWAVWAYSLLKEGNLAYQAYKRICPPNRCSDIDLYSAEP"
BASE COUNT          564 a          524 c          352 g          639 t
ORIGIN      
        1 aaggctctgc agagtaaagg tctatatcgc tgcaacggtt cggcggacag attcttttat
       61 aagcctgata tgcaagattg ccttctttca gtaaagagta agcccatacc gcccatgttg
      121 ctgcgtgggt atacactccg ccattctctc taagccccgg tgcatacctt gtaatatatc
      181 ctatgtcggt tctgggagct gtatatgcag gagccagaag cagtgcgcca taatccttaa
      241 gcagatgttc cgtaacgttt tccatagcag ttatagctct ttgactgtca cctatcgaac
      301 ttatcaaaga ccagagctgc ggattaagaa aaatctttcc ttcttcattc tcattagacc
      361 cgatcttctc aaaaccgtcc gtagtagcct gcagatacca ctttccatcc caaccgtact
      421 tttcaaaaga ctccttgaga gtctgcataa cgtcttccgc tttttttaaa aaagcattgt
      481 ccttgacgta agaaccaaag tccataaact gcccaagaac catatagaaa aattcagaca
      541 tccaaaaact ttcgcctttc cagtcagtac caactgcact cagaccgtca ttccagtcgt
      601 tatcgcccat aagcggtacc ccgcggggac tgaaccttga cataaccttt tcaacagctt
      661 ttttacagtg tatataaaaa gtatcctgac ctccatcata gtacggaaca gtttcatcaa
      721 gaattgaaaa atcacctgtt tcctgtatat aatagtacgc tataaaaaca agccacagaa
      781 gatcatcaga acacttggtt cttggccccc agttaccgat tgtaagccac caatgcaaaa
      841 catctccatc cctgaactgg tgggcagcat gcataagaag ctgtttttta gtcagttccg
      901 gcttacaggt cagaaagatc agcgaatcct gtagctgatc acgaaagcca tatccgcctg
      961 atacctgata atatcccgac ttagcccata ttcttcccga tatggcctga tacttcaacc
     1021 atatattggt cattatatcc atggctttat caggagtttt tatctcgcaa tccaaaaact
     1081 cactccagaa aaccgacagg tttttaaact ccctttcaca tgcctggaca gatgtaaact
     1141 tttctataag ctcagcagga tcttctttgc ctttataggc cgttcctgta gtaaagacta
     1201 ctgttttttc ttcacctgct tgcagcttta tttctacctg tagcgcagct ataggatcgg
     1261 taaatcttcc tgttttcttt ccgagaagat ctttttccat agcctgtggc ttggattcat
     1321 ccctatacat gcctataaac gactctttat cgccatcata cgactttact tcttcgctta
     1381 ctgcatgaaa tgctgtctga tcccagcttc tgttgttgtg gcgacccttc tcgtcaggaa
     1441 aagcacagat gagtttttca gcaagtatgg cgttcttagt aaacttactg tccacaaaga
     1501 tcttgtgaaa ctccctgtgt tcatccggaa aactacccag aacccattca gtatatgacg
     1561 taagatcaag ctttctgatt gtactaccca tattttttac tcttatcctt gctatttcca
     1621 caggatagtt gggtaccaca aaaaataaaa tttctgtctc aatatccttt actctctgca
     1681 gaatgcttgt gtacccaatg ccgtgcctta cctcgtaaaa gtcaaaagca tgcataacag
     1741 gcttcctgcc gacagaccaa tactctttgt cctcaagatc ccttatatag tagtacttgc
     1801 cccagttgtc ctttataaga tcctgatatg accttgttat tctgttttcg ccggcattgg
     1861 atctgaaaga gtagccgctg ccgttttgag atactattat cgaataatct ccgttactta
     1921 tcacgttcac ccatggtctg ggagtctgcg gatcagttat aacatactct gcaccgtttt
     1981 tatcaaagta tccgtacttc gattcaaaaa gcttttgcat ttcaaccatc ctctctaaaa
     2041 gttaaatatt aaactctaca ttacttccct gacagtttt
//
LOCUS       DAOSHR010000308         2066 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_53894_length_2066_cov_4.869717, whole genome shotgun sequence.
ACCESSION   DAOSHR010000308 DAOSHR010000000
VERSION     DAOSHR010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53894_length_2066_cov_4.869717"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1724)
                     /locus_tag="PLU28_11040"
     CDS             complement(<1..1724)
                     /locus_tag="PLU28_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35636.1"
                     /translation="MVKIGIYSQKEGEKTAFLLSTYNYFVQKAKGEKITIGTSNINTE
                     KTLRTLMDQFFSVKTIPGAGKEQNLDFFFDYNGFNSYYRILSYPYTAWDENADLSKKV
                     IHSSDGFIFMIDARSFNDKAYELSVIKTLQTIFNKNKNSYNLPLCILVSDLEISLEYF
                     LKEYFPGIREFLDKYGINYEILSYNSILMETQDPELNESQKIEEVYLWLIRKIFLLEN
                     FTPSTEGRHIPVTTPKDKNSGNALKITITVVAVITILVSLFFLVKDLSINRAGMAENA
                     YLNLFNDYLRSSESKNTDAKHWLNLEEKSTSFKNKTLSKISKNDSLKEKTDSLITDIR
                     ITRIFLSTYYVIKEDFQNMYTSREEDPKKWEELRKRVDSFKDAYEKTHIARFGFSEDF
                     ARIYERTVLITSIIKMRTDYALIFNDLMQGDIFKAEQKLHSYIENYPRALTNEDVSKV
                     NNYISFLSKKNFTVTALNIDLWDYDSQGLSVQLTSVLGSRKYDYGMTKAIEEQCAGDV
                     KIKILKKDDALMTANLSFEDIKNGLEKSFSQPLQYKSLEVDTAVLFKSTPESPAGTAA
                     RPIRQIASG"
     gene            complement(1861..>2066)
                     /locus_tag="PLU28_11045"
     CDS             complement(1861..>2066)
                     /locus_tag="PLU28_11045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35637.1"
                     /translation="VYKGALSRDQNYDITMGGKLYFQKDSLEATVGYWNTTLSELVPK
                     KQTDKKKKLVDMQELLTYMNSTK"
BASE COUNT          581 a          389 c          348 g          748 t
ORIGIN      
        1 ccggatgcga tttgccgaat cgggcgagcg gctgttcctg ccggactttc cggagtactt
       61 ttgaagagaa ctgccgtatc aacctccagg ctcttgtact gcaacggctg ggaaaaggat
      121 ttttccaatc cgttttttat atcctcaaag cttaggtttg ccgtcataag agcgtcgtcc
      181 tttttcagaa tctttatttt tacatccccc gcacattgtt cctcaatggc ttttgtcatg
      241 ccataatcgt atttcctgct tcctagtacg gatgtaagct gaacggaaag tccttgactg
      301 tcataatccc acaaatcaat attcagagct gtaacggtaa aatttttctt tgataaaaaa
      361 gagatatagt tgttaacttt tgacacatct tcattggtga gtgcgcgtgg ataattctca
      421 atataggaat ggagcttctg ttctgcctta aaaatatcgc cctgcatgag atcattgaat
      481 ataagagcgt aatcagtcct catttttatt atggaagtaa tcagaactgt tctttcatat
      541 atccttgcaa aatcttcaga aaagccgaac cgggcaatat gggttttttc ataagcatcc
      601 ttaaaagagt caactctttt tcgtagctcc tcccattttt tgggatcttc ttctctgctg
      661 gtgtacatat tctgaaaatc ttctttgata acatagtacg tgcttaaaaa tatccgtgtg
      721 atccttatat cagttatcag agagtctgtt ttttctttta gggagtcgtt tttgcttatt
      781 ttcgaaagag tcttattttt aaagctcgtg cttttttctt ccagattcaa ccaatgcttt
      841 gcatcagtgt tttttgactc actgctcctg agataatcgt taaaaagatt aagataagca
      901 ttttccgcca ttcccgcacg gtttatggat aaatccttca caaggaagaa taaagaaacc
      961 aatattgtta ttacagcgac taccgttatc gttatcttaa gggcatttcc cgagttttta
     1021 tctttagggg tagtaacggg tatgtgcctg ccttctgtgg agggtgtaaa attttccagc
     1081 aaaaaaatct ttctgataag ccataagtat acttcttcta tcttttgact ttcattaagc
     1141 tcaggatcct gtgtttccat caaaatagag ttgtaactaa gtatttcgta gtttattccg
     1201 tacttatcaa gaaactccct gatgcctggg aagtactctt ttaagaagta ctccaaagat
     1261 atttccaagt cactgacaag aatgcataaa ggaagattgt aactgttctt atttttgtta
     1321 aagattgtct gaagagtttt gattactgaa agctcatatg ctttatcgtt gaaagatctg
     1381 gcatcaatca tgaaaataaa tccgtctgag gaatgaataa ctttttttga caagtcagca
     1441 ttttcatccc atgcggtata agggtaactt aagattctat agtaagagtt gaacccgttg
     1501 taatcaaaga aaaaatccag attctgctct tttcctgcgc cagggatggt tttgaccgaa
     1561 aaaaactgat ccataagagt tctcaaagtc ttttcagtat tgatatttga agtaccgata
     1621 gttatttttt caccctttgc cttctgtaca aaataattat aggtacttaa tagaaaagcg
     1681 gttttttctc cctctttttg gctgtagata ccgatcttta ccattaagtc aactccttat
     1741 tactattttt acttaattat accatgaata aaaaaatttt ttaatatacc atctcggatt
     1801 ttttctccct ccattcttat aacagtaaaa ttcttttttt gtatagataa caaaaatttt
     1861 ctacttagta gagttcatgt aagttaaaag ttcttgcata tccaccagtt tctttttttt
     1921 atctgtctgt tttttaggaa ccaattctga taatgttgta ttccagtatc ctacagttgc
     1981 ttccaaagag tccttttgaa aataaagttt gcctcccata gttatatcat aattttgatc
     2041 tctagataag gctcctttat atacta
//
LOCUS       DAOSHR010000309         2056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_54275_length_2056_cov_5.630685, whole genome shotgun sequence.
ACCESSION   DAOSHR010000309 DAOSHR010000000
VERSION     DAOSHR010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54275_length_2056_cov_5.630685"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            64..1257
                     /locus_tag="PLU28_11050"
     CDS             64..1257
                     /locus_tag="PLU28_11050"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HOG35638.1"
                     /translation="MKIKLLLVYLCTVSIISLSGDFTLYIKNYDPQTSRVDVAVVCNF
                     VTMEDPRITLFSGTFKREIIPKKLGLGSYYFSFYAGSLPKLAKLTAIIRGKDFMQNTR
                     SSSFPDFINLSDFRPSPEINLWAARNNAGEYEYDYSVTTDAGLVLSSISTPESTFTQA
                     IELVQSMENLPDGTYNTVFRFLNYYGMEFSFDVKLMKISDLILSENSIKTEFDEEFIG
                     YYVVSSNDTVSKIATLYSLHPGEIVIANNIKDPSKIFPGQVLKISKILFKDSPLSIKI
                     DISKNKMYLYYHDRLIKNFTVAVGTSDSTPPGEYRIMYREKEPALYWYGEYIRPGSII
                     NGIGSRWLQLSFPQYGIHGTNKPWEIGKRISHGCIRMFNFDVEQIDFIVSLGTQVTVY
                     KSEGE"
     gene            1263..>2056
                     /locus_tag="PLU28_11055"
     CDS             1263..>2056
                     /locus_tag="PLU28_11055"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HOG35639.1"
                     /translation="MLFITTIYRIVPIFLIIFLGFALGKIFKTLDHKLLGKLTLYLFG
                     TIIAFVNVNLNPPSGQDFFNYLFAFLIIFGIFTIMIIILKKLGIYKYDTGVAVNLVTH
                     LNAGYLGYPIITVLYGEEFIRIGVIYSITMTLIMSSIGIVMLSGDIKNGLKNMVKLPL
                     IHGFLAGWLLAAVGVNYQKLPFLVSYPIDMLNDAAITFILIYIGVTLSRVKLSPSNVK
                     QTVLLTALKLLFLPVLGFLAVSIIPMEPMYRKIFLLETAMPTAMNMV"
BASE COUNT          626 a          393 c          387 g          650 t
ORIGIN      
        1 ggaaaggaaa aggttctgca acgattgtcc aatgttattt agatattacc tgcgggtgat
       61 tttttgaaaa ttaaactttt gcttgtttat ctgtgtactg tttctataat ctctttaagc
      121 ggagatttta cattatatat aaaaaattat gatcctcaga cttccagagt ggatgttgcc
      181 gttgtgtgca actttgtcac tatggaagat ccgaggataa ctcttttttc tgggactttc
      241 aaaagagaaa taattccaaa aaaactggga ttgggatcat attacttttc tttttatgca
      301 gggtcacttc caaaacttgc aaaattaact gcaataatac gtggaaaaga ttttatgcag
      361 aatacacgca gttcatcctt tccggatttt attaatcttt ctgatttcag gccttctcct
      421 gagataaatt tatgggccgc cagaaacaac gccggcgagt acgagtatga ctattcggta
      481 actacagatg caggtttggt tctttcatcc ataagcactc cggagtcaac ttttacacag
      541 gccatagagc ttgtccaaag catggagaat ctgcccgatg gtacttataa taccgttttt
      601 cggtttctca attactatgg aatggagttt tcatttgatg ttaaactcat gaaaatatca
      661 gatcttatac tatctgaaaa ttctataaaa acagaatttg atgaagagtt cataggttat
      721 tacgttgtca gctcaaacga tacggtttca aagattgcca cactgtatag ccttcatccc
      781 ggggaaatag taatagcaaa taatataaag gatccttcaa aaatcttccc gggacaagtt
      841 ttaaagattt caaagatcct ttttaaggac agcccactca gtataaaaat agatatttca
      901 aaaaataaga tgtatctgta ctaccatgac aggctcatca aaaatttcac cgttgcagtt
      961 ggaaccagtg attctacccc gcctggagag tacaggataa tgtataggga aaaagaacct
     1021 gctctttact ggtacggtga gtatattcgg ccaggttcca taatcaacgg aatcggaagc
     1081 agatggcttc agctttcttt tccacagtac ggcatacacg gaaccaataa accctgggag
     1141 ataggaaaga gaatatctca tggctgcata agaatgttca attttgatgt tgaacagata
     1201 gattttatag tttctctggg cactcaggta acggtatata aaagtgaagg agaatagacc
     1261 agatgctttt tatcactaca atatacagga tagttcccat attcctgata atttttcttg
     1321 gctttgccct gggaaaaatc tttaaaaccc ttgatcataa acttctcgga aaactcacgc
     1381 tttatctttt cggaacgata atagcttttg tcaacgtaaa tcttaatcct ccttcaggac
     1441 aagatttctt caactactta ttcgcttttc ttataatatt tggaatattc actatcatga
     1501 tcatcatcct taaaaagtta ggaatctata agtatgacac cggagtagcc gtaaatctag
     1561 taacccacct taatgctggg tatctcggat accctattat aacggttctt tacggtgagg
     1621 agtttatacg tataggagtt atatactcta taactatgac acttataatg tccagcattg
     1681 ggatagtaat gctgagcggg gacattaaga acggtctgaa aaacatggta aaacttcctc
     1741 ttatacacgg ttttcttgca ggatggctac ttgccgccgt tggagtgaac taccagaagc
     1801 ttcctttcct tgtaagctat cccatagata tgctgaacga cgccgctata acttttatat
     1861 tgatatatat cggggtaact ctttcaagag taaagctcag tccgtcaaat gtaaaacaga
     1921 cggttctgct gacggcattg aagcttcttt tccttcccgt cctaggcttt cttgccgtaa
     1981 gcataattcc tatggagcct atgtatagaa agattttcct tcttgaaacc gccatgccta
     2041 cagctatgaa tatggt
//
LOCUS       DAOSHR010000310         2056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_54286_length_2056_cov_3.764118, whole genome shotgun sequence.
ACCESSION   DAOSHR010000310 DAOSHR010000000
VERSION     DAOSHR010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54286_length_2056_cov_3.764118"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="PLU28_11060"
     CDS             <1..273
                     /locus_tag="PLU28_11060"
                     /inference="COORDINATES: protein motif:HMM:NF025879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HOG35640.1"
                     /translation="KDIFYYIASSIDGKIPSGLCSCSIKPSTWVIFSCEGDFRLSVQN
                     IFRVFFTQWLVFSGYEYANLPDIEVYPLDSKKSNNCEVWIAVKKRT"
     gene            286..1248
                     /locus_tag="PLU28_11065"
     CDS             286..1248
                     /locus_tag="PLU28_11065"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG35641.1"
                     /translation="MYTIQTQDLSKTYKNGVNALNSLNIQVKEGEVFSLLGPNGAGKS
                     SLIKILTTQCLATKGKAFILGIDTQKNPSSIRPLISCVSQSISIDSHMSLKENFIFQA
                     RLYSLDNKTIKQRMTTLIDSFKLSEYVKYPVSTYSGGIKRRLDIAMNMISFPKVLFLD
                     EPTTGMDVQSRRAMWDMIQKIKETFRTTIFLTTHYLEEADILSDSICIIKDGKELIQA
                     SPYELKRLTGNSMIKIGLTDAKLLPSCCRYIESLKLCSSSYIRGESAFFVSDDSDSSF
                     PLIIRALLDKPFYVKSADITSPSLEDVFIDLISKKEVKEDKKVC"
     gene            1242..1988
                     /locus_tag="PLU28_11070"
     CDS             1242..1988
                     /locus_tag="PLU28_11070"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG35642.1"
                     /translation="MLNILYRNMKWRVQNPITIVITLIQPILWMVLYGTVGANSMHST
                     ISNYNAFILPGIMVLVTFSACCSGGIINYIMKNNASFYRVLISPVKRVSVVSGQALET
                     VILSFIEISVLMAVSLFFSVRVPSDPLSFLSMLIILFLTAFFLSCLTYTMSLLLPNEV
                     VYETVMNAIVLPTFFLSSALFPSETLSKNLYIVIYLNPFSHVINILRKILMNEPFPAR
                     EFFSVSLLLLILCIITFSCALYRLKKETAK"
BASE COUNT          671 a          406 c          364 g          615 t
ORIGIN      
        1 aaagatattt tttattatat tgcatcgtct atagacggaa aaattccctc cggtctttgt
       61 tcctgcagca taaaaccctc aacttgggta atattcagct gtgaaggtga tttcagactg
      121 agtgtgcaga acatattcag agtttttttt acccaatggc ttgttttttc aggatatgag
      181 tacgcaaacc ttccggatat agaagtttat ccgttggaca gtaaaaagtc aaataactgc
      241 gaagtctgga ttgcagttaa aaaaagaaca taaaggagaa atagcatgta tacaatacaa
      301 acccaggatc tttcaaaaac ttacaaaaac ggcgtgaatg ccttgaacag cctgaacata
      361 caggtaaagg aaggtgaagt tttttcgctt ttaggcccta acggtgccgg aaaatcatcg
      421 cttataaaga ttcttacgac tcaatgcctc gcaacaaaag gaaaagcttt tatactggga
      481 atagacaccc aaaagaaccc tagcagtata agacctttaa tatcatgcgt ttcacaaagc
      541 atatctatag atagccatat gtccctaaag gaaaatttta tctttcaggc aagactttat
      601 agtcttgaca acaaaacgat aaaacaaaga atgactacgc tcattgatag ttttaaactt
      661 tcagagtatg taaagtatcc ggtatcgaca tactccggcg gaataaaaag aaggctagat
      721 attgccatga atatgatatc ctttcctaaa gtcctttttc tggacgaacc cacaacaggc
      781 atggatgtac aatcaagaag agccatgtgg gatatgatcc agaaaataaa agagaccttc
      841 agaaccacca tatttctcac cacccactac cttgaagaag ccgatattct aagcgacagc
      901 atctgtataa taaaagacgg aaaagaactt attcaagcaa gtccgtatga acttaaaaga
      961 ctcacaggaa attctatgat aaagatagga ctgacagatg caaagctttt gccctcctgt
     1021 tgccggtaca ttgaaagttt aaagctctgt agttcttcat atatccgcgg agagtctgca
     1081 ttttttgttt ctgatgattc agacagcagc tttccactga taattagagc acttttagat
     1141 aaaccttttt acgtaaaatc ggcggatata accagtccaa gccttgaaga cgtttttata
     1201 gatcttattt ccaaaaaaga agtaaaggag gataaaaaag tatgctgaat atcctgtaca
     1261 gaaatatgaa gtggagagtc caaaacccaa taaccatagt tatcacattg attcagccca
     1321 tactttggat ggttctttac ggaacagtcg gagcaaacag catgcacagt accataagta
     1381 attacaatgc ctttatactt cctgggataa tggttttggt aactttttcc gcatgctgca
     1441 gcggaggaat aataaactat atcatgaaaa acaacgcaag cttttacaga gtactcatat
     1501 ctccggtaaa aagagtatcc gttgtatcgg gacaggcact tgaaactgtt attctgtctt
     1561 tcatagaaat atctgttctc atggccgtat ctcttttttt ctctgtcaga gttccatcag
     1621 acccgctcag ctttttatct atgctgataa tactttttct aacagccttt ttcctttcat
     1681 gcctcacata caccatgagt cttttgctac cgaatgaagt ggtatatgag actgttatga
     1741 acgctatagt actcccgaca ttttttctga gctcggcgct tttcccttct gaaactctct
     1801 ccaaaaactt atatatagtc atttatctta atccgttttc tcacgtgata aatatcctga
     1861 gaaaaatact tatgaatgaa ccgtttcctg caagagagtt tttttctgtt tcattacttc
     1921 ttctaatcct atgtattatc actttcagct gtgcacttta caggcttaaa aaagaaacag
     1981 caaagtaaaa ccggcacata tgcgtcggca ctttgtttat attattttca gaacggatat
     2041 acattagaat gtgcag
//
LOCUS       DAOSHR010000311         2051 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_54495_length_2051_cov_5.361222, whole genome shotgun sequence.
ACCESSION   DAOSHR010000311 DAOSHR010000000
VERSION     DAOSHR010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54495_length_2051_cov_5.361222"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1224
                     /locus_tag="PLU28_11075"
     CDS             <1..1224
                     /locus_tag="PLU28_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35643.1"
                     /translation="YDYEKIAEGIYVVGSLDTPSEISILLNKPNVVKLDNITTDYIKD
                     VLNQFGERVKYIEGADFIVVYGKDQVAQKAIDIIRQLDKLGTGYTFIYNVYSVSQSAF
                     TAIQKLNYFDFPIKYANSLNFLTRNFTRLTTLVEIGYLQMDKSAQFEFTKDFSTLKGK
                     VAVDKNQFNISTEIVTSGESSGVMTSAQNINEPIYATYVIGGKFYVVSFNLMPKLEVS
                     LSKDARSYASVSAGIYLDQTLKPCLYSSVNMNSGSISFLYDFKDYFNLNFVSSIVSDL
                     QGIISLTADNKGKVYMKAGIGETQKIDAFSLYGEFLLNTMLSFEKSEISPSFTGLEYN
                     LSVAWRILQDYGLLKYLEAGGGVDFSYLMPNSYTIRPHAFISAKADILLPLTFQAQAG
                     LDLKYKIAAFATVEF"
     gene            1266..1934
                     /locus_tag="PLU28_11080"
     CDS             1266..1934
                     /locus_tag="PLU28_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOG35644.1"
                     /translation="MFNILIVEDDKSIARLLELELSHEGYNTKIAYDGEQAVQFYENF
                     KPDVILLDIMLPKMNGFEVAKAVRDYDKDVGIIMLTAKGELEDRVHGLKSADDYVVKP
                     FEIEEVLARIKSLLRRLGKSKELLKAGSIEIYPEKMQVFVNENEIHLSLTEFNILKLL
                     VINKDLVVSKEKIMEEVWGYSEDENLNVVEVYVNYLRKKLGDSSDYIKTVRGVGYVIR
                     EIEQ"
BASE COUNT          668 a          361 c          426 g          596 t
ORIGIN      
        1 tacgactatg aaaagattgc cgaaggaata tatgtagtag gctctcttga tacaccatct
       61 gaaatttcaa ttcttctaaa caagccaaat gttgttaaac ttgacaacat cacaacggat
      121 tacataaaag atgtactgaa ccagttcggt gaaagagtaa agtatataga gggagccgat
      181 tttatagtgg tttacggtaa agatcaggtt gcccagaagg ctattgatat catacgccag
      241 ctggataaac tcggaacagg atataccttc atatataacg tctactctgt atcccagagc
      301 gctttcacag ctatacagaa gctgaattac tttgattttc ccataaaata cgccaactcc
      361 cttaactttc tcacaagaaa ctttaccagg cttactacac ttgttgagat aggatatctg
      421 cagatggata aaagtgccca gtttgagttt actaaagatt ttagtactct taaaggcaaa
      481 gtcgcggtag ataaaaatca attcaatata tcaactgaaa tagtaacctc aggagaaagt
      541 tccggtgtta tgaccagcgc tcagaacata aatgaaccta tatatgcaac atacgttata
      601 ggcggtaaat tttacgtagt ttcgttcaac ctcatgccca agcttgaggt aagtctttcc
      661 aaagatgcaa gaagttacgc aagcgtaagt gccggtatat acttggatca gactcttaag
      721 ccatgtcttt attcttctgt caatatgaac tctggaagta tctcgttctt atatgatttt
      781 aaagattatt tcaaccttaa ttttgtgagt tcgatagtat ccgatcttca gggaataatc
      841 tctttgacag cagacaataa aggaaaggtt tatatgaaag caggaatagg agaaacacaa
      901 aaaatcgatg cttttagcct ttacggagag tttttgctta acactatgct tagttttgaa
      961 aaatctgaga tctcaccttc ttttaccggt ctggaataca atctttccgt tgcttggagg
     1021 atattgcagg attacggtct tctcaagtat cttgaagcag gaggaggagt ggatttttcg
     1081 taccttatgc ccaacagtta cactataaga cctcatgcat ttataagcgc caaggcagat
     1141 atactcttgc ctctgacatt tcaggcacag gcaggtcttg acttaaagta taaaatagca
     1201 gcatttgcca cagttgagtt ttaaaagacg ccccggatgg ggtgtctttt tgaataggag
     1261 gagatatgtt taatattctt atagtcgaag atgacaaatc tatagcaagg cttctggaac
     1321 ttgagctctc acacgagggt tataatacaa agattgcata cgacggagaa caggccgtac
     1381 agttctatga aaattttaag cctgatgtga tattattaga catcatgctt cccaaaatga
     1441 atggctttga agttgcaaaa gctgtgagag actacgataa agatgtaggc ataataatgc
     1501 ttacagctaa gggtgagctt gaagacaggg ttcacggact gaaaagcgcc gatgactatg
     1561 tggtaaaacc ttttgagata gaagaggttc ttgctcgtat aaaatcattg ttacgaagac
     1621 tcggaaagtc aaaggaactg cttaaagcag gcagtatcga aatataccct gaaaaaatgc
     1681 aggtctttgt aaatgaaaat gagattcatc ttagccttac agaattcaac atattaaagc
     1741 tccttgttat taacaaagat cttgtggtca gcaaggagaa gattatggaa gaagtatggg
     1801 gatatagcga ggatgaaaat ctcaacgttg ttgaggttta tgtaaactac ctccgtaaaa
     1861 agctcgggga cagttccgac tatataaaaa ccgtgcgagg tgttggctac gtcatcaggg
     1921 agattgaaca ataacaaaat aagttccgtt ttgagactca caataatttt cacagtcgcc
     1981 atgatatttg tagtggtata tgtttccctt ttcctgaggg taattgttgc caatgaatct
     2041 gtgaaaacct a
//
LOCUS       DAOSHR010000312         2050 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_54533_length_2050_cov_3.682707, whole genome shotgun sequence.
ACCESSION   DAOSHR010000312 DAOSHR010000000
VERSION     DAOSHR010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54533_length_2050_cov_3.682707"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1035
                     /locus_tag="PLU28_11085"
     CDS             <1..1035
                     /locus_tag="PLU28_11085"
                     /inference="COORDINATES: protein motif:HMM:NF015728.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RAMP superfamily CRISPR-associated protein"
                     /protein_id="HOG35645.1"
                     /translation="YITRPKIGKRDGVKLELFNKIAVDQSKYDYEILYPGQKFDIKIE
                     VIIREKHEKYEQAFRNYLAQLLDGLKNSKISIGAKTSRGFGKIKIGENQSITVLDMNK
                     KQDIETWINFDWKTFPSNTTLANLGTAVQHEQYITIETELLIEKTLMIRSYAQDPNEE
                     SDYRHITEEGKGIIPGTSWAGAFRHHIAKLLLDLNATDYEKIIEELFGYVDEKTKTAR
                     RSSIIFEESKITKAKSLYMTRVKIDRFTSGAYDSALFNENPNIKGKTKLTIKVRKDKE
                     KYIGLIIMAIQDLKSGIMSLGGETSIGRGTFTATKDSKLKIDGEFIGKEKETEYLQQA
                     AELIMAAQGA"
     gene            1037..1585
                     /gene="csx19"
                     /locus_tag="PLU28_11090"
     CDS             1037..1585
                     /gene="csx19"
                     /locus_tag="PLU28_11090"
                     /inference="COORDINATES: protein motif:HMM:TIGR03984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Csx19"
                     /protein_id="HOG35646.1"
                     /translation="MKITTIKTTKETKELTQEQIIPEINQIFQDKTAYVLQYTSHSVD
                     IGYIKNNTITLYKDQQFIPSDFTDVRIFNEEMELHILKKKEKIIARLRKDGQGEDTDI
                     FDQKVMLLGETQKILNEEWTEFSETRGASLILPLKDAQAQNTFYKARNYIQYTNDGEL
                     CFKDARLCGFYNTNEEKIRSDI"
     gene            1585..>2050
                     /locus_tag="PLU28_11095"
     CDS             1585..>2050
                     /locus_tag="PLU28_11095"
                     /inference="COORDINATES: protein motif:HMM:NF015728.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RAMP superfamily CRISPR-associated protein"
                     /protein_id="HOG35647.1"
                     /translation="MPGYNGNRSYNNNNGSQGGKPRWAETTAFTNPYNFITLGNKCMR
                     KPYEEYTKSPLKTGWIECTLTTKTPLFIPNTTNEDAFRINKKDHKSYDFFSYDDIKGQ
                     DRTKEFSEPVIPASSIRGAMRAMYEAVTDSCMSTADDEKILYKRTPIPKLPGM"
BASE COUNT          849 a          418 c          374 g          409 t
ORIGIN      
        1 tatataacca gacccaaaat tggaaaacgt gatggagtaa aacttgaact ttttaataaa
       61 atagccgttg accaatccaa gtacgactac gaaatactct atcccggtca gaagtttgat
      121 ataaaaattg aagtaataat aagagaaaaa cacgaaaaat acgaacaagc cttcagaaac
      181 tatctggcac agctgctgga cggacttaaa aattccaaaa taagtatagg tgcaaaaact
      241 tcaagaggct ttggaaagat aaaaatagga gaaaatcaga gcataacggt tcttgacatg
      301 aacaaaaagc aagacataga aacatggata aactttgact ggaaaacatt tccatccaat
      361 actacattag caaatctcgg aactgcagtt cagcatgagc agtacataac catagagaca
      421 gaactgctga ttgaaaaaac acttatgata cgctcgtatg cccaagaccc aaacgaagaa
      481 tcagactaca gacacataac ggaagaagga aaagggataa ttcccggaac cagctgggca
      541 ggagccttta gacaccatat agccaaactg cttttagatc ttaacgcaac agattacgaa
      601 aaaatcattg aagaactctt tggctacgta gacgaaaaaa ccaaaacagc ccgcagatcc
      661 tccataatat ttgaagaatc aaagattacc aaggctaaaa gcctttacat gaccagagta
      721 aagatagacc ggtttacctc cggcgcatat gactccgcac ttttcaatga aaatcctaac
      781 attaaaggaa agaccaaact taccataaaa gtcagaaaag acaaagaaaa gtacatcggt
      841 cttatcatta tggccataca ggacctaaaa agtggaataa tgagtttggg aggagaaacc
      901 tcaatcggaa gaggaacatt tacagcaaca aaagactcaa aactcaaaat tgacggtgaa
      961 tttataggaa aagaaaagga gaccgagtac cttcaacagg ctgcagagct tataatggca
     1021 gcacaggggg cataaaatga aaataactac cataaaaacc acaaaagaaa ccaaagaact
     1081 cactcaggaa cagataatac ccgagataaa ccagatattc caggataaaa cagcatacgt
     1141 tcttcaatac accagccata gcgtagacat aggatacata aaaaacaata caattacctt
     1201 atataaagac caacagttta tcccatcaga ctttacggac gtaagaatat tcaatgaaga
     1261 gatggaacta catatcctaa agaaaaaaga aaaaataatt gccagactca gaaaagacgg
     1321 tcagggagaa gacacagata tatttgatca aaaagtaatg ctgctaggag aaacccaaaa
     1381 aatactcaac gaagaatgga cagagttttc agaaaccaga ggagccagcc tgattcttcc
     1441 gctgaaagat gctcaggctc aaaacacatt ttacaaagcc agaaactata tccaatatac
     1501 aaacgacggc gaactttgct tcaaagacgc tagactttgc gggttttaca acaccaatga
     1561 agagaaaata aggagtgata tataatgccc ggatacaatg gcaatagatc atataacaat
     1621 aacaacggtt cacaaggagg aaaacccagg tgggcagaaa cgacagcatt taccaatccg
     1681 tacaacttca taacccttgg aaacaaatgc atgagaaaac catacgaaga gtataccaaa
     1741 agcccattaa aaacaggctg gatagagtgt acccttacca ccaaaactcc gctgtttata
     1801 cccaatacca ctaacgaaga tgcctttagg ataaataaaa aagatcataa atcatacgac
     1861 ttcttcagct acgacgatat aaaaggacag gacagaacca aagagttttc tgaaccggtt
     1921 attcctgcaa gctccataag aggtgctatg agagcaatgt acgaagcagt cacagactca
     1981 tgcatgtcta cagccgatga cgaaaagata ctctacaaaa gaaccccaat acctaaactt
     2041 ccgggcatgc
//
LOCUS       DAOSHR010000313         2040 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_54947_length_2040_cov_3.916877, whole genome shotgun sequence.
ACCESSION   DAOSHR010000313 DAOSHR010000000
VERSION     DAOSHR010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54947_length_2040_cov_3.916877"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1055)
                     /locus_tag="PLU28_11100"
     CDS             complement(<1..1055)
                     /locus_tag="PLU28_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HOG35648.1"
                     /translation="MFVYFDNNATTPVDKEVADLIYKYMVSDYANPNSIHGFGNKIEK
                     MMEDARELIAEKLDVMPYEIYFTSCASESINWAIRGICKANFRSGKHIVSSAIEHSAT
                     INTLKELENDGYTVTLIKTDKSGVIDLKELQQAIREDTVLVSIMAANNETGTLEPVEK
                     IADIIKSKNKNTYFHVDGVQAIGKIPFSIRKVNCDMASFSAHKFHGPKGVGISYKKEG
                     IRIFPYLTGGSQERGMRGGTQNTAGIIGSALAMKKAYDNLEDMKRIDTLRHFMAEKIT
                     QMGGIIATPLDNSVPNTLAVIFEGVRADSIVSALSDEGIFVSTSSACSSKSSSSNRVM
                     HQMGYTEKQAQGNIRISL"
     gene            1121..1945
                     /locus_tag="PLU28_11105"
     CDS             1121..1945
                     /locus_tag="PLU28_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOG35649.1"
                     /translation="MKKAYFWLILITIVWGTTFPLQKLALNGISTSTYISLRFWIASI
                     VSFVVWRKHSFKYGVILGLVFAGSFIAQTYGLSLTTASKNGFITSLYIIMVPFFSYII
                     EKEKPSVFQYFGFPMALLGSYFMAGGLSGFNLGDLLTFISAVGYAVHIVLLTIISKKV
                     DETSILSFQFLTVAVITTFMSIGSGVQYNYLSVSVAAYTAIFATVIATFLQARYQKVV
                     SSNTAALIFVGEPIFSAFFSFLILKEQLSSRQFFGAAILIGALVFASFRKKQQQPL"
BASE COUNT          561 a          401 c          369 g          709 t
ORIGIN      
        1 aggctaattc gtatatttcc ctgagcctgt ttttctgtat atcccatctg atgcatgaca
       61 cggtttgaag aactgctttt gctcgaacat gctgaagaag ttgaaacaaa tattccttca
      121 tcagacagag cagaaactat cgaatcggca cgcactcctt caaagattac agcaagagtg
      181 ttaggaactg aattatccag gggggtagct ataattccac ccatctgagt tatcttttct
      241 gccataaaat ggcgcagagt atctatgcgt ttcatatctt ccagattatc gtaagctttt
      301 ttcattgcca gagcagagcc tataataccg gcagtatttt gggttccgcc tctcatacct
      361 ctttcctgcg aaccaccggt aagataagga aaaattctta ttccctcttt tttgtaggat
      421 atgcctacac ctttgggacc atggaactta tgggcagaga agctagccat atcacaattc
      481 actttccgga tgctgaaagg tattttccct atagcctgta ccccgtctac atgaaagtaa
      541 gtatttttat tttttgattt tattatatcc gctatctttt cgacaggttc tagagtacct
      601 gtttcattgt tggcggccat tatgcttaca agaacggtat cttctcttat tgcttgctgc
      661 agttctttaa gatctatcac accagacttg tctgttttta taagtgtgac agtgtaacca
      721 tcattttcaa gctcttttag agtatttata gttgcgctgt gttcaatcgc agaggacact
      781 atatgcttgc cgctcctgaa attagcttta catatgcctc ggattgccca gtttatagat
      841 tcgcttgcac aggatgtgaa gtatatttca taaggcatta catcaagttt ttcagcaatt
      901 aattctcttg catcctccat cattttttca atcttattgc caaaaccatg aatggagtta
      961 ggattagcat aatctgaaac catatattta taaataaggt cagctacctc tttatcaaca
     1021 ggtgtggttg cattgttgtc aaaataaaca aacataaaag aacctccttt ttaaaaagtt
     1081 ttatagtata attaatgaaa aaataatggg gtgaaagatc ttgaaaaagg catacttttg
     1141 gttaatcctt ataacgatcg tctggggaac aacatttcct cttcagaagc ttgctctgaa
     1201 cggaatttca acaagtacgt atatttcttt gcgtttttgg atagcaagta tcgtgtcttt
     1261 tgtggtctgg aggaagcatt ctttcaaata tggagtaatt ctaggacttg tatttgccgg
     1321 ttcatttatt gcccagactt acgggctttc acttaccaca gcctcaaaaa atggattcat
     1381 aacgtctctg tatataataa tggttccatt tttttcatac ataattgaaa aagaaaaacc
     1441 atctgttttt cagtactttg gattcccgat ggctttgtta ggttcttact ttatggccgg
     1501 aggactttca ggatttaact taggtgatct cctgacattt atcagtgcag taggatatgc
     1561 ggtgcatata gttttattaa caattatatc aaaaaaagtt gatgaaactt ctatactttc
     1621 ttttcaattt cttactgtag cggttataac cacttttatg agtataggat cgggagtgca
     1681 atacaattat ttatcggttt ccgttgcagc atataccgca atttttgcta cagtgatagc
     1741 aacatttctt caggcacggt accaaaaagt tgtaagcagt aacactgctg ctctaatctt
     1801 tgttggggag cctatatttt cagctttttt ttcttttttg attcttaaag aacagctttc
     1861 ctcccggcag ttttttggag ctgctattct tataggagct ctcgtatttg cctcattcag
     1921 aaaaaaacaa cagcagccac tttaaacaga cggctgctgt attttcatca tgcttgcttt
     1981 cataaatacc gcggttgcct gaacacatac ctttccggat tcatcagtaa tagaggcttc
//
LOCUS       DAOSHR010000314         2037 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_55074_length_2037_cov_4.260848, whole genome shotgun sequence.
ACCESSION   DAOSHR010000314 DAOSHR010000000
VERSION     DAOSHR010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55074_length_2037_cov_4.260848"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..143)
                     /locus_tag="PLU28_11110"
     CDS             complement(<1..143)
                     /locus_tag="PLU28_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004900359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOG35650.1"
                     /translation="MSRIEDVAKLAGVSTATVSRVLNNTSNVSEDTRQKVMKAIEELD
                     YTPS"
     gene            519..1874
                     /locus_tag="PLU28_11115"
     CDS             519..1874
                     /locus_tag="PLU28_11115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35651.1"
                     /translation="MKKKVFTGIVIFVLILAANVLGATKEFVVQTSSFSGAEVPYVIS
                     FSKMLEMVGDNFEANWYSIRVYDENNKELPYQIEDIDMNRKISAGDKLAFMFRSKAKI
                     VVSDDSDMDLVEYAPVFNIEEKDGIYMVSTDKFTVKVDNHALVSFVKYGQNEGTLFNE
                     LGIVRVAGWSMNTYYTDGKLGKHEENVSAGFDVQSFKILQAGPVCVTAQALMTTKLFP
                     GYNQELTVHIFKNGDVLTENRFFFTNYVNLMKLQIMATAPVTSYDEGAVHILPMFRRM
                     IWTDQLNSTPYEYWLERNAVKMVENKPYITFAAIDSVKPFWWGAAYIFSSMESWRANF
                     SEKYSIGAAEILPVKPLVYGDMEKFVNGDTWVYESREFRDGIFRWMPDEFNVYESTKG
                     VFLPYSDENVGNQWMAKFKANDEVIYTRIYSMFDAKNTEDAVIYLENKTKDIQGIKLS
                     K"
BASE COUNT          703 a          304 c          412 g          618 t
ORIGIN      
        1 gatggagtat aatcaagttc ttctatagct ttcattactt tttgacgtgt atcttcggac
       61 acatttgaag tgttgtttaa aactcttgat actgttgctg tggatactcc tgcgagtttt
      121 gctacgtctt caatgcgtga catttctcac ctctttgtaa gcgctttcat tcaaaacaat
      181 aataccattt tttttacttt ctaaaaatac atacaaaagg tttaaaaaag caaaaaaata
      241 gtaaaaaaag aatataaaca tgaataaaat catataatag catataatca gcttaaaaac
      301 caaaaataat tactgtacgc tatataaaaa gctcatttat tgcttttaca taaagatttt
      361 aaccaaaatg aagatatgca aagaaattgt ataataatca gtattagatg actctaataa
      421 ctataataca gtgtttgcaa aaaacactaa agttttataa aataatatca caaatgtaag
      481 cgctttcata acaaaaaata tcaggaggcg aattggggat gaaaaaaaag gtatttacag
      541 gcatagtcat ttttgtgtta attcttgctg ctaatgttct gggtgcaaca aaggagtttg
      601 tggttcagac ttcttcattt tcaggagccg aggttcctta tgtaatatct ttttcaaaaa
      661 tgcttgaaat ggtaggagat aactttgagg ccaactggta ctctattagg gtatacgatg
      721 aaaataacaa ggaattgccc taccagatag aagatattga tatgaacaga aaaatttcag
      781 caggtgacaa acttgctttt atgttcagat caaaagccaa aatagttgtt tctgatgatt
      841 ctgacatgga tcttgtcgag tatgctccgg tattcaatat cgaggaaaaa gacggaatat
      901 acatggtttc aacagataag tttacggtaa aggtcgataa ccatgcgctt gtaagctttg
      961 tgaagtatgg acagaacgaa ggaacgcttt ttaatgaact tggaatagta agagttgctg
     1021 gatggtctat gaatacgtat tatactgacg gtaagcttgg aaagcatgaa gaaaatgttt
     1081 ccgcgggctt tgacgtacag agctttaaaa ttcttcaggc aggtcctgta tgtgtaaccg
     1141 ctcaggcact tatgacaacg aagctttttc cgggatataa ccaagagctt acagtgcata
     1201 tatttaaaaa cggtgatgtt cttacggaaa atagattctt tttcactaac tatgtaaatc
     1261 ttatgaagct tcagataatg gcaacggcac ctgttaccag ttatgatgaa ggagccgttc
     1321 atattcttcc gatgttcaga aggatgatat ggacagatca gcttaactct actccttatg
     1381 agtactggct tgaaagaaat gcggtgaaga tggttgaaaa taaaccgtat ataacttttg
     1441 cagccataga ttcagttaaa cccttctggt ggggagcagc atatatattc tcaagcatgg
     1501 aaagctggag agctaacttc tccgaaaagt attctatagg agcggctgaa atacttcccg
     1561 ttaagccttt ggtctatggg gatatggaaa agtttgttaa cggcgatact tgggtatatg
     1621 aaagcagaga gttcagagat ggaatattcc gatggatgcc agatgagttc aatgtttatg
     1681 agagcactaa gggtgtattc ctgccatact ccgacgagaa cgtaggcaat cagtggatgg
     1741 caaagttcaa ggccaatgac gaggtcatat atacaagaat ttattctatg tttgatgcta
     1801 aaaacacaga agatgcagta atatatcttg agaacaagac aaaagatatt cagggaatca
     1861 agctttcaaa ataatagaaa ataaaaaatt tagggcaagt ttacaagact tgccctaaac
     1921 tgttagagga ggaaggatga aaagattttt tttaatcgtg attataggta tggttatggg
     1981 tatatcagct ttttcaaaga tagatataaa tctttcgcat ctggagtttt taaaaga
//
LOCUS       DAOSHR010000315         2034 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_55185_length_2034_cov_5.240020, whole genome shotgun sequence.
ACCESSION   DAOSHR010000315 DAOSHR010000000
VERSION     DAOSHR010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55185_length_2034_cov_5.240020"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            21..1265
                     /locus_tag="PLU28_11120"
     CDS             21..1265
                     /locus_tag="PLU28_11120"
                     /inference="COORDINATES: protein motif:HMM:NF021596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HOG35652.1"
                     /translation="MKKAFWCLLVLAFIPMMIFPLSTDHFEEEFRKSFDFFWEQANSI
                     PGSVGYGLVRDRYPGSAECASIASTGFGLSAIVIGVEKEWITYEQGAERAEKTLDTLL
                     KMETVRGFFYHFVDISTAQRINKSEISTIDTAILLCGALTCAKYFGGEILSKTMMLFS
                     EINWKVFVDKSTNYFYMSLMPESGFSGRWDFYAEQLMMYVLGAASPTYPIGKEVYYAF
                     KRNRSSYKGSTFIHSWFGSLFTHQYSHAWIDFRNKKDAMGVNWHQNSVDASLANYRYC
                     LDLKDRYPHFSESCWGLSACDSISGYNGLYGSQPSGYDNKANVVDGTIAVSAALGSIV
                     FTPEESLRALDYYYSIDGLWGRYGLKNAFNPCQNWIAKDYIGIDKGITLLMFSNYENG
                     LIWSLFSDIDCIKEGLKKLEIN"
     gene            1282..>2034
                     /locus_tag="PLU28_11125"
     CDS             1282..>2034
                     /locus_tag="PLU28_11125"
                     /inference="COORDINATES: protein motif:HMM:NF021596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HOG35653.1"
                     /translation="MGRNDIVQTEAKLSFDFFWNEASLMENSYGLIKDNTVHDAASIA
                     SVGFGLSAIVVGVNRGWITREEGYKRVVGTLKTFYSKAEQKEGFFIHFLDMQTARRTW
                     HSEVSVIDTAIFLMGALCAAEYFKGQAEEYFEKIYQRVNWQWYTNKKKNMFYMGYWYE
                     NGFHGAWDLYAEQLMMYILGAASPSFPIDPSMYYEFKRNTGCYRDYQLIYTWTGSIFT
                     YQFTHAWIDFRKLYEKNGVNWYENSVNATKAAR"
BASE COUNT          602 a          348 c          492 g          592 t
ORIGIN      
        1 ggagaataga gggggagttt atgaaaaaag cattttggtg tttactggta ctggctttta
       61 ttccgatgat gatctttcct ttaagtacag atcattttga ggaagagttc agaaagtcct
      121 tcgatttttt ctgggaacaa gctaacagta taccaggttc tgtgggttat ggtttggtgc
      181 gtgacagata tccggggtct gctgagtgtg cgagtattgc gagtacaggc tttggactta
      241 gtgccatagt aattggagtc gaaaaagaat ggatcaccta tgaacagggg gctgaaaggg
      301 ctgaaaaaac gcttgataca cttttaaaaa tggaaactgt gcgtggtttt ttttatcatt
      361 ttgtcgatat aagtactgcg caaagaataa ataaaagtga aatctcaacc attgacactg
      421 cgatactgct gtgtggtgct cttacatgtg caaagtactt cggcggagag attctttcta
      481 agacgatgat gcttttctcg gaaataaact ggaaagtatt cgtagataaa agtaccaact
      541 acttttatat gtcgctcatg cccgagtcag gattttccgg ccgatgggat ttttatgctg
      601 aacagcttat gatgtatgtt ctcggcgcag cttctcctac ttatcccata ggcaaagagg
      661 tatactatgc ttttaagcgg aacaggtcat cgtataaggg cagtacattt atacactcgt
      721 ggtttggttc actctttacc catcaatact cacatgcatg gattgacttt cgcaacaaaa
      781 aagatgctat gggagttaac tggcatcaga actctgtaga tgcaagtttg gctaattaca
      841 ggtactgtct ggatctgaag gacaggtatc cgcatttttc agaaagctgc tggggacttt
      901 cggcttgtga cagtataagc ggatacaatg gactttacgg ttctcaacca agcggatacg
      961 ataacaaggc caatgtagtt gacggaacta ttgctgtatc cgcagctctt ggcagcattg
     1021 ttttcacccc tgaggagtcc ttgcgtgcgc ttgactatta ctactccata gacggattat
     1081 ggggcaggta cggattgaag aatgctttta atccttgcca gaattggata gcaaaagatt
     1141 atatcggaat agacaaggga ataacgcttt taatgttttc taactatgag aatgggctca
     1201 tctggagtct cttttctgat attgactgca taaaagaagg tttaaaaaag ctggaaataa
     1261 attagcgtat agggaggaag tatgggaaga aacgatattg ttcaaaccga agcaaaactg
     1321 tcttttgatt ttttctggaa tgaagcatca cttatggaaa actcttatgg tcttataaaa
     1381 gataatacgg ttcatgatgc ggcaagcata gccagtgtgg gatttgggct ttctgcaatt
     1441 gtcgtgggtg taaatagagg ttggataacc agagaagagg gctacaaaag agttgttggt
     1501 actcttaaga ctttctacag caaggctgag cagaaagaag ggttcttcat acactttctg
     1561 gatatgcaga ctgcccgaag aacttggcac agcgaagttt ctgtcataga taccgccatc
     1621 ttcctcatgg gagcgctttg tgccgcagag tactttaaag gtcaggctga ggagtacttc
     1681 gaaaagatat accagagggt aaactggcaa tggtatacaa acaagaaaaa aaatatgttt
     1741 tacatggggt actggtacga aaatggattt catggggcgt gggatcttta cgctgagcag
     1801 ctcatgatgt atattttggg agcagcatcc cccagctttc ctatagaccc aagtatgtat
     1861 tatgagttta aaaggaatac aggatgttac cgggattatc agcttatata tacatggacg
     1921 ggttctatct ttacatatca gtttacacat gcatggatag acttccgaaa actttatgaa
     1981 aaaaacggag taaactggta tgaaaactct gttaatgcaa ctaaagccgc aaga
//
LOCUS       DAOSHR010000316         2012 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_56110_length_2012_cov_3.759836, whole genome shotgun sequence.
ACCESSION   DAOSHR010000316 DAOSHR010000000
VERSION     DAOSHR010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56110_length_2012_cov_3.759836"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="PLU28_11130"
     CDS             complement(<1..581)
                     /locus_tag="PLU28_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOG35654.1"
                     /translation="MNRPIQVTRSSMPPMDEYTDEIKDLWDTHWLTNMGVKHHKLEQM
                     LKNYLKTPYITLFTNGHLALECIFSALKLTGEVITTPFTFASTTHAIVRTGLKPVFCD
                     IREEDCTIDPDRIESLITKNTCAIVPIHVYGNLCDTERIEEIARKYNLKVVYDAAHAF
                     GVSRYGKSSACFGDASMFSFHATKVFNTIEGGAV"
     gene            complement(574..1140)
                     /locus_tag="PLU28_11135"
     CDS             complement(574..1140)
                     /locus_tag="PLU28_11135"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOG35655.1"
                     /translation="MTSFYNVEELKEFGFAKVGINVLISRKASIYGAENMSIGSNVRI
                     DDFCILSGKITIGNYVHIGAYSAIFGSFGVEMHDFSGISSRVSIYSSSDDYSGEYMTN
                     PTVPQKYRGCVNAKVVLSKHVIVGAETVILPGVVIGEGTAVGSMCLVNRSCDEWSIYG
                     GIPAKRIKERNRHILELEKELLKEKKHE"
     gene            complement(1154..>2012)
                     /locus_tag="PLU28_11140"
     CDS             complement(1154..>2012)
                     /locus_tag="PLU28_11140"
                     /inference="COORDINATES: protein motif:HMM:NF026018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOG35656.1"
                     /translation="WHPKKVFSLERVKVLFSFGWKLLASALLETFYANIRSLIIGKLY
                     TPATLGFYNRGDQFPQIIVSNINGSIQAVIFPALSSEQDNIEKIKNMVRRAIMMSSFL
                     IFPMMTMLAVTAKPLVIVLLTEKWLPCVPFIQIFCASYALWPMHTANLQAINALGRSD
                     IFLKLEVIKKIFGTLILVVSIFYGIKAIAYGMLFSSIMFSFVNAYPNLKLLNYSYIEQ
                     LKDIIPSLIISLIMGFLIHLFSYLNMSNWMTLVIQTLTGIFLYLGLSKIFKVESYKYL
                     ILMFSEIRK"
BASE COUNT          635 a          420 c          309 g          648 t
ORIGIN      
        1 acagctccgc cttctatcgt attaaagact tttgtggcat ggaaactgaa catagatgca
       61 tctccaaaac atgcagaact tttgccatat ctgcttacac caaaagcatg cgcagcgtca
      121 taaacaacct ttagattata cttcctggct atctcctcta tcctttctgt atcacagaga
      181 tttccataaa catggatggg aactatggca caagtatttt tagttataag actttctatt
      241 ctgtcaggat caattgtaca gtcttcttct ctgatatcgc aaaagacggg tttaagtccg
      301 gttcttacaa tagcatgggt tgtggaagca aatgtaaaag gagtagtgat tacttcccca
      361 gtaagcttca aagccgaaaa tatacattcc agagccagat gaccgtttgt aaataaggtt
      421 atatatggcg ttttcagata atttttcagc atctgttcaa gcttatgatg ttttactccc
      481 atatttgtaa gccagtgtgt atcccataaa tcttttattt catccgtata ctcatccatc
      541 ggaggcatgg atgatcttgt tacctgtatc ggcctattca tgttttttct ccttcagaag
      601 ctctttttcc agctcaagga tatgcctgtt tctttccttt attctttttg caggtattcc
      661 tccatatata gaccattcat cacaacttct gttaacaagg cacatactgc ccactgccgt
      721 tccctcccca atgaccactc cgggaagaat aaccgtttct gctccaacta tcacatgctt
      781 tgacaataca acttttgcat ttacgcatcc acggtatttt tgaggaactg taggattggt
      841 catatattct cccgaatagt catccgaaga actgtatata ctcactctgg aggatatgcc
      901 agaaaaatca tgcatttcaa cgccaaaact tccaaatata gcactgtatg cccctatatg
      961 cacataattt cctatcgtta tctttccgct tagtatgcaa aagtcgtcta tccgcacgtt
     1021 gctgcctata ctcatatttt cagcaccgta tatgcttgct tttctgctta taagtacgtt
     1081 aattccgact ttcgcaaatc caaactcctt gagctcttct acattgtaaa aactggtcat
     1141 ttctaatctc ctttcacttt cttatttctg aaaacatcag aattagatac ttgtaacttt
     1201 caactttaaa gatctttgaa agtcctaaat ataaaaatat tcctgtcaac gtttgaataa
     1261 ctaaagtcat ccaattagac atattcagat aagaaaataa atgaattaaa aagcccatga
     1321 ttaaggatat gatcaaagaa ggaattatat cttttagttg ttcaatataa ctgtagttaa
     1381 gcaattttaa gttgggataa gcattcacaa aggaaaacat tatactgctg aaaagcatcc
     1441 cataggctat agctttaatt ccgtaaaaaa tagaaacgac cagaattaaa gtaccgaata
     1501 ttttttttat aacttccagt ttcaaaaaaa tatcgcttct gcctaaagca tttatagctt
     1561 gaagatttgc cgtatgcatt ggccacaaag catatgaagc acagaatatc tgaataaacg
     1621 gcacgcaagg cagccatttt tccgtcaaca aaactattac cagtggttta gcagtaacag
     1681 caagcatagt catcataggg aaaattaaaa aagaactcat cattatagcg cgtcttacca
     1741 tattttttat cttttctata ttatcctgtt cagaagaaag tgccggaaaa attactgctt
     1801 gaatagaacc gttaatgttt gaaactataa tctggggaaa ttgatctcct ctgttataaa
     1861 aacctaaagt tgccggagta tacagcttac ctattataag acttcttatg ttcgcataaa
     1921 acgtttccag caatgctgaa gcaagtaatt tccagccaaa agaaaataat acctttaccc
     1981 tttccaatga aaaaactttc ttaggatgcc aa
//
LOCUS       DAOSHR010000317         2011 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_56149_length_2011_cov_3.828221, whole genome shotgun sequence.
ACCESSION   DAOSHR010000317 DAOSHR010000000
VERSION     DAOSHR010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56149_length_2011_cov_3.828221"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            132..1016
                     /gene="vanW"
                     /locus_tag="PLU28_11145"
     CDS             132..1016
                     /gene="vanW"
                     /locus_tag="PLU28_11145"
                     /inference="COORDINATES: protein motif:HMM:NF033128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein VanW"
                     /protein_id="HOG35657.1"
                     /translation="MKKKRITQMFPWLLPLRINQRLLFFYTGMYFDGNSYASFISEQK
                     LKYKIFEAGSLLYNHQTGFDMQYQENKVFNLKLAAQTLNNLIIRPQETFSFWKLVRYA
                     DKNIPYKDGLVMVDGKLEVSAGGGMCQMSNLLFWIFLHTPLTIVERHGHSKKDFPDTS
                     EDMPRGVDATVAEGWLDLKVKNETDQSFQIAIDFDEKNIIGRIFSDVKLDYSYCISNA
                     KSFYYRENTKIFEEVDVIQKKISADKGECICSKVLYRNKCEIGYPLEPEIKISEKGRE
                     KYGKIKYRRHIWRLFPRV"
     gene            964..>2011
                     /gene="vanG"
                     /locus_tag="PLU28_11150"
     CDS             964..>2011
                     /gene="vanG"
                     /locus_tag="PLU28_11150"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: protein motif:HMM:NF002528.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HOG35658.1"
                     /translation="MERLNIAVIFGGCSPEYSISLKSAHAVISAMDKSRFMPLIIGVS
                     SKGDWFKFDGSHDKILNDTWCNSNDCTPALFSPCRSEKAVLVFNNDRVEKLKIDAAFP
                     VMHGKNGEDGTIQGLLELSGIPIIGCGALCSALCMDKYRAHMLVKSFGINVPASFLFD
                     KTTDIEKIFEQAEKIHYPLYIKPLRAGSSYGISKVWSAGELPDAVKKAFEYDDQAVAE
                     ENIQGYEVGCALMGNEKIVIGQVDEIRVPQGFFSYEEKYTPKSSIIKVPADISHGKAQ
                     EIKETALCIYRILGCHGFARVDMFIDMDEKIVFNEVNTIPGFTSRSRFPNMFKACGIT
                     FEKLISDIIRLGLEA"
BASE COUNT          677 a          326 c          422 g          586 t
ORIGIN      
        1 tgtaagaaaa acgtatgatt tttataatta aataacaagg gttttgtcat ataaaacgaa
       61 agcatcgtaa gttccgtctt ggtattattt tacaagacgg aactttttta tatacagaaa
      121 ggaataaaaa tatgaagaaa aaaaggatta cacaaatgtt cccttggctg ctgccattaa
      181 gaataaacca gcgtctattg tttttttata cgggaatgta ttttgatgga aacagctatg
      241 catcctttat atcagagcag aagcttaagt acaaaatttt tgaggcaggc agcctcttat
      301 acaaccatca gaccggattt gatatgcagt atcaggaaaa taaagtattt aatctcaaac
      361 tggcggctca aactctcaat aatcttataa taagaccgca ggaaactttt tctttctgga
      421 agctggtacg gtacgcagac aagaatatcc cgtataaaga cggactggtt atggttgacg
      481 gaaaactcga agtatcggct ggcggaggaa tgtgtcagat gagcaacctt cttttttgga
      541 tttttttgca taccccactt acgattgtag aacgccacgg acatagcaag aaagattttc
      601 cagatacttc agaggatatg cccagaggag ttgatgcaac agttgcggaa gggtggcttg
      661 atctgaaagt caaaaacgaa accgatcaat catttcaaat agctattgat tttgacgaaa
      721 aaaacattat aggacgtatt ttttcagatg tcaaattaga ttattcctat tgtatttcaa
      781 acgcaaagag tttctactac cgtgaaaata ctaaaatttt tgaagaagtc gatgtaattc
      841 aaaaaaaaat ctctgctgat aaaggagaat gtatctgttc aaaagttctt taccgtaata
      901 aatgcgaaat agggtatccg cttgaacctg aaataaaaat aagtgagaaa ggaagagaaa
      961 agtatggaaa gattaaatat cgccgtcata tttggcggtt gttccccaga gtatagcata
     1021 tcgctaaaat ctgcccatgc tgttataagt gccatggata agagcagatt tatgccattg
     1081 atcatagggg tgtcttcaaa aggtgattgg tttaagtttg atggcagtca tgataaaata
     1141 ctgaacgata catggtgcaa ctccaacgat tgtactccgg cattattttc tccctgccgt
     1201 tcagaaaaag ctgtattggt ttttaataac gatagagttg aaaagcttaa aatagacgcg
     1261 gcctttccgg ttatgcatgg gaaaaacggc gaagatggaa cgattcaagg attgctggag
     1321 ctttcaggta ttcccataat tggctgcgga gcgctttgtt cagcattgtg catggataaa
     1381 taccgagcgc atatgcttgt aaaatctttt ggcataaatg ttcctgcctc gtttttattt
     1441 gataaaacaa cagatataga aaaaattttt gaacaagctg aaaaaattca ctatccgcta
     1501 tatataaaac ctttaagggc aggctcttca tatggtatct ccaaagtgtg gtctgccgga
     1561 gaactgcctg atgcagttaa aaaagctttt gaatatgacg accaggctgt agcagaagaa
     1621 aatatccagg ggtatgaagt cggctgtgca ttaatgggaa atgaaaaaat tgttatagga
     1681 caggttgatg aaataagagt tccgcaaggt tttttcagtt atgaagagaa gtatacgcct
     1741 aaatcatcaa taataaaagt tcctgccgat atttctcatg gaaaagcaca ggaaataaaa
     1801 gaaacagccc tatgcatcta tagaattttg ggttgccacg gttttgccag agtggatatg
     1861 ttcattgata tggatgaaaa aatagtattc aacgaggtga atacaattcc tggttttact
     1921 tcccgcagtc gttttccaaa tatgttcaaa gcctgcggaa taacttttga aaaactcatt
     1981 tctgatataa tccgactggg gttggaagca t
//
LOCUS       DAOSHR010000318         1977 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_57647_length_1977_cov_4.551509, whole genome shotgun sequence.
ACCESSION   DAOSHR010000318 DAOSHR010000000
VERSION     DAOSHR010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57647_length_1977_cov_4.551509"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..930
                     /locus_tag="PLU28_11155"
     CDS             <1..930
                     /locus_tag="PLU28_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOG35659.1"
                     /translation="HIINILNIILTPVVWVFISISNVFIKIFGGKTIVSAPFITEDEI
                     LGYLDLGHEEGVIEKHEKLLMQRSLEMREISVKEVMSPRIDIVALEDTQTIKNLIDLI
                     NDEGYSRIPVYRDSLDSIVGICYAKDVFKILNQNDSYEKVKNLPVTQIMHKPYFVPMT
                     MKVKDVLKMILANRVHMSIVVDEYGGTAGLVTLEDVLEELTGEIMDEYDDVKEETQFF
                     RIDDSTLLCKGTTPINDLEREFDKEFPETEYETLSGFVLEKFERFPTPGEKLSVDDFE
                     FEVVAVSMNRIEKVKVHCTVNDEKTAGSDDDDL"
     gene            914..1339
                     /locus_tag="PLU28_11160"
     CDS             914..1339
                     /locus_tag="PLU28_11160"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016289952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HOG35660.1"
                     /translation="MMMIYKTQDEVIRQLYEQAVRVRENAYVPYSGFKVGAALLGKSG
                     KIFTGCNVENVSYGLTVCAERNAFFKAVSEGETSFTVLLVVADTPEPVSPCGACRQVM
                     AEFGDFEVILSNLSYQIKRMFVHELLPYSFDKDNLNVGK"
     gene            1326..>1977
                     /locus_tag="PLU28_11165"
     CDS             1326..>1977
                     /locus_tag="PLU28_11165"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG35661.1"
                     /translation="MLANSDMTYTVLIIDDSPINRMFIKTVVEDGLVGFKVIECENGR
                     HGLSMAREKNPDLILLDIMMPEMDGYTVCGLLKNDPSTKEIPVIFLSALSEIEDKTKG
                     FEAGGVDYITKPADPREILARVFAHARLYRTQKSLLDYINRIDQELSIAQIVQFELLP
                     KNLRVNDLKMEWLYQISNNVSGDIFGVIPYDQQNSIVYMVDVSGHGVASAMRSTMIK"
BASE COUNT          636 a          322 c          460 g          559 t
ORIGIN      
        1 cacataataa atattcttaa tatcatcctt acccctgtgg tttgggtatt tataagtata
       61 tcgaacgtat ttataaaaat tttcggagga aaaaccattg tatccgctcc atttataact
      121 gaagatgaga tactgggtta tcttgacttg ggacatgaag aaggagtaat agaaaagcat
      181 gaaaagcttc tcatgcagag aagccttgaa atgcgtgaga tatcagttaa agaggttatg
      241 tctccgagaa tagacatagt tgcccttgaa gatacacaga ccataaaaaa cttaatagat
      301 cttattaacg atgaaggata ctcaaggata ccggtttaca gagattcact agattcgata
      361 gtgggtatat gttatgccaa ggatgttttt aagatactta atcagaatga cagttatgaa
      421 aaggtgaaaa atcttccggt aacgcagata atgcacaaac cttactttgt tccgatgacc
      481 atgaaggtca aggatgtact gaagatgatc cttgcaaaca gggtgcatat gtccatagta
      541 gttgatgaat acggcggaac agccggactg gtgacacttg aggatgttct tgaagaactt
      601 accggagaga taatggatga gtacgatgac gttaaagaag aaacccagtt tttcagaata
      661 gatgattcta cacttctttg taaaggaact actcctatta acgatcttga aagagagttt
      721 gacaaggagt ttccggaaac tgaatatgaa acattgagcg gatttgtgct tgaaaagttt
      781 gaacgttttc caacacccgg tgaaaagctg agcgtcgatg attttgagtt tgaagtagtg
      841 gctgtttcca tgaacagaat agaaaaggta aaggttcatt gtacggtcaa tgacgaaaaa
      901 acagcaggaa gtgatgatga tgatctataa gacgcaggat gaggtaataa ggcagttgta
      961 tgaacaagct gtaagggtgc gtgaaaacgc ctatgttcca tactccgggt ttaaagtggg
     1021 ggcagctttg ttgggaaaaa gcgggaaaat ttttaccggc tgcaatgttg aaaacgtttc
     1081 ttacggtctt actgtctgtg cagagcgtaa tgcctttttt aaagctgttt ctgaaggtga
     1141 aacatctttt actgttttgc tggtggttgc cgatactccc gagcctgtaa gcccttgcgg
     1201 tgcatgtaga caggtcatgg cagagttcgg agattttgag gtaattcttt ccaacctgtc
     1261 atatcagata aaaagaatgt ttgttcacga gcttttgcca tactcttttg acaaggataa
     1321 tcttaatgtt ggcaaatagt gatatgacct atacggtgct tataattgat gattcgccta
     1381 taaatagaat gttcataaaa accgtcgttg aagacggact tgttggtttt aaggtgatag
     1441 aatgcgaaaa tggccgccat ggcttaagca tggccagaga aaaaaatcct gatcttattc
     1501 ttttagatat aatgatgcct gagatggacg gctatacagt atgcggactg cttaaaaatg
     1561 atccgtccac taaggaaata ccggtaatat ttttaagtgc gttgtctgaa atagaagata
     1621 aaaccaaggg atttgaagcg ggaggtgtcg actatataac caagccggcc gatcccagag
     1681 aaattcttgc aagggtcttt gcacatgcca gactttacag gacgcaaaaa tcacttttag
     1741 attacataaa taggatagac caagagctta gcatagctca gatagtacag tttgaactcc
     1801 tgcccaaaaa tcttagggta aacgatctta aaatggaatg gctttatcag atatccaata
     1861 atgtttcagg ggatatattc ggagtgatac catatgatca acagaacagt attgtttata
     1921 tggtcgatgt ctcaggacac ggagtagcct ctgcgatgag gtcgaccatg ataaagc
//
LOCUS       DAOSHR010000319         1977 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_57655_length_1977_cov_3.336108, whole genome shotgun sequence.
ACCESSION   DAOSHR010000319 DAOSHR010000000
VERSION     DAOSHR010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57655_length_1977_cov_3.336108"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(184..1536)
                     /locus_tag="PLU28_11170"
     CDS             complement(184..1536)
                     /locus_tag="PLU28_11170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35662.1"
                     /translation="MIALNQVRQMMNIRDAKIFRFLDHGDNLLLPTQAGIQKSKDLIN
                     LYFKYIPFDEDCRMNCVFTFKNQDISFIRELITDEVFSFLTENIFLLDSGFTVIDTKS
                     QKTFPEPEVTLTLKERTELNHESDYESIKKVLLHINAPVYTERRIRSILVSGENTFRI
                     KPYNLRFIGELFKYGIFEGIELKCPALNPELGKIILLREEENMEGFSERLTKTVGNNY
                     LFFSFSDGQEMMDFAEKTLKDMYREFELLMPTDRCELTDCRFVFPNRENSNSSKLELK
                     TFLENFSIPFSNRFLQQIRDTMIDFDIQSYNFDTADKMLDLIISAHKTLDRIYYFMNS
                     INPNFPNTISYALENAMDVVAGFEKKYSLVLLIPSKRQEISQRISSLKDKASMKKFKI
                     EHALDIIKPLIVAMGEISDLCLRNSSMIKRDLKLSELEKELYELLKNELIEMTKPAVV
                     "
     gene            complement(1548..>1977)
                     /locus_tag="PLU28_11175"
     CDS             complement(1548..>1977)
                     /locus_tag="PLU28_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35663.1"
                     /translation="DVSKVTNYISFLSKKSFILTALNFDLLDSIKEELSVQLTSARGT
                     KTYAYGMTQSIEEHCADYVKIKIIKKDDTLMSASISFEDVKKGLEKSFSQPLQQKNLK
                     VDTAVLFKTTTESTAGTATFSIGFEIDLFLKNPANYKIFN"
BASE COUNT          595 a          356 c          354 g          672 t
ORIGIN      
        1 ttgatatcta taaaggaaaa ggaatcgatc cggataagat gagcataacc ataacaatag
       61 tttacaggtc ttttgaaaaa actcttaccg atgaggacgt aaataaagtt gaatcaaaaa
      121 tgcttaccaa gctttcacag atggaagtag tactgagagg ggcataataa aaaaaccggc
      181 agttcacacc actgccggtt ttgtcatttc tataagctcg tttttaagaa gttcgtagag
      241 ttccttttcc aattccgaaa gtttcagatc tctttttatc atagaggagt tacgcagaca
      301 aaggtcactt atctcaccca tggctactat aaggggtttt ataatatcca gtgcatgttc
      361 aattttgaat tttttcattg atgctttatc tttaagtgaa gatattctct gacttatttc
      421 ctgacgtttt gatggaataa gaagtaccag ggaatacttt ttttcaaacc cggcaacaac
      481 atccatagca ttttccagag cataagagat tgtgttgggg aagtttgggt ttatgctgtt
      541 cataaaataa tatatccggt caagggtttt atgggcgctt ataataagat ccagcatttt
      601 atcggccgta tcaaagttgt agctttgtat atcaaaatct atcatagtat cgcgtatctg
      661 ttggagaaat ctgttagaaa aagggattga aaagttttcc agaaaagttt tcaactcaag
      721 cttactgcta tttgagtttt cacgatttgg aaatacaaac ctacagtcgg taagttcgca
      781 gcggtctgtc ggcattaaaa gttcaaactc tctgtacata tctttaagag ttttttctgc
      841 aaaatccatc atctcctggc cgtctgaaaa agaaaaaaac aaataattgt ttccaaccgt
      901 ttttgtcagc ctttctgaaa aaccttccat attttcttct tctctcaaaa gtattatctt
      961 tcccagttca ggattcaagg ccgggcattt cagctctata ccttcaaata taccgtattt
     1021 gaaaagttca cctataaatc ggagattgta aggctttatc ctgaaagtgt tctctccgct
     1081 tacaagtatt gaacggattc ttctttctgt atataccgga gcgtttatat gcaacagaac
     1141 cttttttata ctttcatagt cggactcatg attaagctcg gttctttcct tcagcgtaag
     1201 cgtgacttcc ggttccggaa aagttttttg tgattttgta tcaataactg taaatccact
     1261 atccagaaga aatatatttt ctgtcaaaaa cgaaaaaacc tcatctgtaa tgagttctcg
     1321 tataaaagag atgtcttggt ttttaaatgt aaaaacacag ttcatcctgc agtcctcgtc
     1381 aaagggaata tatttgaaat aaagatttat aagatctttt gacttttgta taccagcctg
     1441 ggtaggaagc aaaaggttat ccccgtggtc aagaaatctg aagattttag catcacggat
     1501 gttcatcatc tgtcttacct gattcaatgc aatcatatgc aagcccctca gttaaatatc
     1561 ttataatttg ccgggttttt aaggaaaaga tctatctcaa agcctattga aaaggttgcc
     1621 gttcccgcag tactttctgt agtggttttg aataaaacag ccgtatcgac tttcagattt
     1681 ttctgttgca gaggttggga aaaggatttt tccaatcctt tttttacatc ctcaaagctt
     1741 atgcttgcgc tcataagagt atcatctttt tttattatct ttatttttac ataatctgca
     1801 caatgttcct ctattgactg tgtcatgcca taggcatagg tcttggtccc tcttgcagat
     1861 gtgagctgga ctgaaagttc ttctttaatg gagtctaata aatcaaaatt caaagctgtg
     1921 aggataaaac ttttttttga taaaaaagag atatagttag taactttcga aacgtct
//
LOCUS       DAOSHR010000320         1950 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_58868_length_1950_cov_4.246438, whole genome shotgun sequence.
ACCESSION   DAOSHR010000320 DAOSHR010000000
VERSION     DAOSHR010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58868_length_1950_cov_4.246438"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            228..1178
                     /locus_tag="PLU28_11180"
     CDS             228..1178
                     /locus_tag="PLU28_11180"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOG35664.1"
                     /translation="MYKYIIILSFLFFAVPLFIPVDPLAGTVDEKVLADEDSLFLSVG
                     DIDVHYKMKQGDPKRNFILLHGFGSNLWTWEKISKPLSKLGTVVSYDRPAFGLTSRPL
                     SFVEKDPYSYEYQPEIFFQLMDRLKMEKTFIVANSAGADIAVRAYFTAPSRIDGIILI
                     APSLGDNNSSFSFFSKLAEVPQYERLGPLISRSLKDSGESIIKSAWYDPSRISEKDLE
                     GYRKNILINNWDTALWSFTKASFYRTSAGRVEDIRCPVLVLSGDSDVIIPVDKSRELA
                     RRIQNSSFRVIEKCGHLPQEERPDELMAVIIEFIASLDSD"
     gene            1197..>1950
                     /gene="nudC"
                     /locus_tag="PLU28_11185"
     CDS             1197..>1950
                     /gene="nudC"
                     /locus_tag="PLU28_11185"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF001299.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HOG35665.1"
                     /translation="MQTGKVYRCVFCEDKILVKTVNGRLALPLENDIQNMKNEITCTR
                     KLQDIFGNIIHVYETLNAQERPDFFFSSMRPLLAEFKPDEFSAVARAYQKINWNKKNN
                     FCRRCGADISAENPDGSKTCSKCGLVVYPEISPAIIVAVTKGERLLLAHNVNFKSNMF
                     SIIAGFVEMGESLEDCVRREVMEEVGLQIKNIRYFSSQPWPFPNGLMIGFTAEWESGD
                     ITPDGCEIHDARWFVPSEFPNLPMGGSISMMLI"
BASE COUNT          590 a          319 c          430 g          611 t
ORIGIN      
        1 tatgtctatt tccgttttct gcaagatatg aaaagatggt ataaggaata tttgaaaaac
       61 agtacttctc ataatcaggc tttatgaatc cttcaaaagt cattgtttta aggtgctcaa
      121 tggatttttt gtttatcatg gtttcctcct gtaagtataa ttattcttat taactatatt
      181 atatacgatt ttctggttta acagactaat aagatcgggg tggaagtatg tataagtaca
      241 taatcatatt gagttttttg ttttttgcag ttccgttgtt cattcctgta gatccgcttg
      301 caggaactgt agatgaaaaa gttctggcgg atgaagacag tcttttttta tctgtcggag
      361 atatcgatgt tcattacaag atgaaacagg gggatccaaa aaggaatttt attttacttc
      421 atggattcgg atctaatctt tggacttggg agaaaatatc caagcctctt tctaaacttg
      481 gaacggtggt ttcttatgac aggcctgctt ttggtcttac gtcaagacct ctttcttttg
      541 tggagaaaga tccttattct tacgaatatc agccggaaat attttttcag ttaatggaca
      601 gacttaaaat ggaaaaaacc tttattgttg caaactctgc cggcgcagac attgctgtta
      661 gggcttattt tacggctcca tcaaggatag atggcataat ccttattgct ccttctttgg
      721 gagataataa cagttcattc tctttttttt caaagctagc agaagttcca cagtatgaaa
      781 gattgggtcc gctcataagc cgttctttaa aagacagcgg agagagcatt ataaaaagcg
      841 catggtatga tccctcaagg ataagtgaaa aagatcttga aggatacagg aaaaatattc
      901 ttatcaacaa ttgggatact gctttatgga gctttaccaa agcttcattc tacagaacct
      961 ctgcgggaag agtagaagat ataagatgtc cggtgctggt tttgagtggg gattccgatg
     1021 ttataatacc tgtagataaa agccgggagc ttgcaagaag aatccaaaac tcttctttca
     1081 gggtgataga aaaatgcgga catcttcccc aagaggaaag acctgacgag cttatggcag
     1141 taataattga atttatagca agtctggatt cagattgaaa tattaaggag aataagatgc
     1201 agacgggaaa agtatacaga tgtgtttttt gcgaagataa aatacttgta aagacagtga
     1261 acggcagatt agctcttccg ctggaaaacg atattcagaa catgaagaat gagattacct
     1321 gcacaagaaa acttcaggat atttttggta atatcatcca tgtgtatgaa actcttaatg
     1381 ctcaggaaag acccgatttc tttttttcgt ccatgagacc tctccttgcc gagtttaagc
     1441 cggatgagtt ttcggctgtg gcaagagcat atcaaaaaat aaactggaat aagaagaata
     1501 atttctgccg cagatgtgga gctgatatct ctgctgaaaa tcctgatggt tcaaaaactt
     1561 gttccaagtg tggtcttgtg gtttatcccg aaatatctcc cgccattatt gtggctgtta
     1621 caaagggaga aagactgctt cttgcgcaca atgtgaactt taaaagtaat atgttcagta
     1681 tcattgcagg ttttgtagaa atgggggaaa gtcttgagga ttgtgtcaga agggaggtaa
     1741 tggaagaggt tggactacag ataaagaata taaggtattt ttccagccag ccatggccat
     1801 ttcctaacgg tctcatgata ggctttactg cagaatggga aagcggtgat ataacacctg
     1861 acggctgtga aattcatgat gccagatggt ttgtgccttc agagtttccg aatcttccta
     1921 tgggcggcag tatttcaatg atgcttatac
//
LOCUS       DAOSHR010000321         1942 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_59231_length_1942_cov_3.148384, whole genome shotgun sequence.
ACCESSION   DAOSHR010000321 DAOSHR010000000
VERSION     DAOSHR010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59231_length_1942_cov_3.148384"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1211)
                     /locus_tag="PLU28_11190"
     CDS             complement(<1..1211)
                     /locus_tag="PLU28_11190"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HOG35666.1"
                     /translation="MKRLLLVVLSLMMVIILVAAPVKLTILHMNDTHGHMWTYGDKNG
                     TGGFERIQAIISMVKSKAAEENGHVLVLHAGDVNTGIPESDQLNAIPDFLGLSVMGVD
                     AMCPGNHEFDKEADVLALQQMVASFPIISSNYVDEKGNTIFKPYIIKDFGDLKVGIIG
                     ATTEQTLILEPIHLGTNTFKNVSESVAKYLPEVRKQADVVVVLGHLGYNEKPVFPIKY
                     TTSDMLAQEVSGIDAIIDGHSHTVIDKKTVINGTLIGQAGEWGKYIGKMDLWVENGKV
                     VNSEYSLIPIDAQIPSDPLIARLAKSYQEKGAEKLNVVIGKTDIVLDGERSSVRAKDT
                     NLAHLITDSFIWKTGADFALTNGGGIRASIPVGNITYKSVLTVLPFGNTVSIVEMTGK
                     DVIDLVTYAAS"
     gene            complement(1296..>1942)
                     /locus_tag="PLU28_11195"
     CDS             complement(1296..>1942)
                     /locus_tag="PLU28_11195"
                     /inference="COORDINATES: protein motif:HMM:NF014046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ARMT1-like domain-containing protein"
                     /protein_id="HOG35667.1"
                     /translation="SNRLLLEMYDDLLQHCINSPDPIEEALKISALGNFIDYGVKNSF
                     GELEWELQNLVKTKTLTINHTKNFIKDLKEARSLLVIHDNAGEIVLDKVLIRIIKKLY
                     PKLVVYSAVRSEPVINDVTMADAQEINLKEVSIVIESGSKYPGTLLDNVNVAFKNIFD
                     NADIILSKGQGNFEGLNGVERNIYFNFMAKCHPVANAVGVKVGDVVFGRIKAHF"
BASE COUNT          532 a          418 c          360 g          632 t
ORIGIN      
        1 atggaggccg cataggttac caggtcaata acatcttttc cggtcatttc gacaattgat
       61 actgtgtttc caaaaggaag aactgtaagg acgctcttgt aagttatgtt tcctacagga
      121 atggacgctc ttattcctcc gccgttagtt aatgcgaagt cagcgccggt tttccatatg
      181 aatgaatcgg ttatgagatg ggcaaggttg gtatccttag ctcttacact tgatctttcg
      241 ccatcaagga cgatatctgt ctttcctatg accacattga gtttttctgc acctttttcc
      301 tgatatgatt ttgcgagtct tgctataagg ggatcggaag gtatctgtgc atctatagga
      361 ataagactgt attcggagtt tacaaccttt ccgttttcaa cccacagatc catctttcct
      421 atatactttc cccattctcc tgcctgaccg ataagagtac cgttgattac tgttttctta
      481 tcgatgactg tatgagaatg accgtcaatt atggcatcta tcccggaaac ttcctgtgca
      541 agcatatctg aagttgtgta ctttatagga aacacaggtt tttcattgta tccaaggtgt
      601 cccagaacaa caacaacatc agcctgcttt cttacttctg gaaggtactt tgcgactgat
      661 tctgaaacgt tcttaaaagt atttgtaccc aggtgtatag gttcaagaat aagcgtctgt
      721 tcggtagttg cgcctattat acctaccttg agatctccga agtcttttat tatgtaaggc
      781 ttgaatatgg tgttcccttt ctcgtcaaca tagttgctgc ttattatagg gaaggaagct
      841 accatctgct gcagggcaag gacatcggct tctttgtcaa actcatggtt tcccggacac
      901 atagcatcca cacccataac acttagccca agaaagtcag gaatggcatt gagctggtct
      961 gattcaggta ttccggtgtt tacatcacca gcgtgaagaa caaggacatg cccattttct
     1021 tcagcagctt tggactttac catggatatt atggcctgga ttctttcaaa accgcctgtg
     1081 ccatttttat ctccgtaagt ccacatatgt ccatgtgtat cgttcatatg aagaatagtc
     1141 agttttacag gagcagccac gagaatgata accatcatca ggctcagaac taccagtaac
     1201 aatcttttca tcttgcaccc cctaaattga gatatcgtta acagtttatt ttatcaaaat
     1261 aatgttataa taaaattaag attttaaaac aacaactaaa aatgagcttt tatcctccca
     1321 aaaaccacat ctccgacttt tacgccgaca gcgttggcta ccggatgaca tttagccata
     1381 aagttaaagt aaatgtttct ctctactcca ttaagccctt caaagttacc ctgtcccttt
     1441 gaaagaataa tatctgcgtt atcaaagata tttttaaatg caacatttac attgtccagc
     1501 agggtacccg gatactttga gccgctttct atgactatgg aaacttcctt gaggtttatt
     1561 tcctgcgcat ctgccattgt gacatcattt attacaggtt cgcttctgac agcagagtat
     1621 accaccagtt tgggatagag tttttttatt attcttatca agaccttatc taaaacaatt
     1681 tcgcctgcat tatcgtgaat aaccagcagt gatcttgctt cctttagatc cttaataaaa
     1741 tttttggtat gatttattgt aagcgttttg gtctttacaa gattctgaag ttcccactca
     1801 agttcaccga aagagttttt taccccgtaa tctataaagt ttcccaaggc tgatatctta
     1861 agagcttctt ctattggatc tggagaattt atacaatgct ggagcagatc atcatacatc
     1921 tccaaaagaa gacggttgga ag
//
LOCUS       DAOSHR010000322         1931 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_59767_length_1931_cov_3.312367, whole genome shotgun sequence.
ACCESSION   DAOSHR010000322 DAOSHR010000000
VERSION     DAOSHR010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 1931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1931
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59767_length_1931_cov_3.312367"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1039
                     /locus_tag="PLU28_11200"
     CDS             <1..1039
                     /locus_tag="PLU28_11200"
                     /inference="COORDINATES: protein motif:HMM:NF024941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4127 family protein"
                     /protein_id="HOG35668.1"
                     /translation="LSTTVYSQESMIEWQNMNTYLKAFYSKDFEKASALKTKINPDIM
                     KQYNLLRKRNLENNLKAVELVSNGSIDFLLLPQEDCFPNGPQTQEQLTISEKILSLNL
                     SEKVFLHNGADEAAQELVSKVANPGEKKISVLCDCPSKLNEIMEFEDRPFQTNLFSHM
                     KALNFIQDNDSQTLLYIAFAEPQTCVRHIKTAIAYGKKVILLDLIAPNGGSKELISEM
                     IRHDLLRQLWGYSAWNTASNSLGTALAICALTNMKTERKKISELLARNLVDDFLYQRI
                     YRQVIDERFKKDIHNMQTVDKKNREDIKKEFISCANKFLNESGFNGLKITDFYFPWER
                     TFECGVTCSYQ"
     gene            complement(926..1588)
                     /locus_tag="PLU28_11205"
     CDS             complement(926..1588)
                     /locus_tag="PLU28_11205"
                     /inference="COORDINATES: protein motif:HMM:NF039476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6512 family protein"
                     /protein_id="HOG35669.1"
                     /translation="MYKEKDKKTLFYWEVGGFFFVCLIGVLFHYIFSYAGNFFLLTLF
                     FALNESIWEQLKLVFWPTLFWGLVESIFIKKKTNNFFAAKVFAATFMVVFIISGFYLY
                     TSFSGHIQAMDIFIVIISVALGQMLGYRIFRCRKFSDTVYKISIAIVAVWAFLFLWFS
                     FNPLKNDIFRAYQITSKKIVKDVLLVRTRDTALKSSFPREVEICNFKSVETAFIEKFI
                     CT"
     gene            complement(1670..>1931)
                     /locus_tag="PLU28_11210"
     CDS             complement(1670..>1931)
                     /locus_tag="PLU28_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35670.1"
                     /translation="TIRIFFMPVIGRQEFSRIIELRCLYLQKYTSHLSRQYEEISKQK
                     LPENVFYAVKYSLDIALANLESAQTILKSLQNHEGNYDFWSI"
BASE COUNT          693 a          370 c          315 g          553 t
ORIGIN      
        1 actgtccaca accgtttact cacaggaatc tatgattgaa tggcagaaca tgaacactta
       61 tcttaaagct ttttactcta aagattttga aaaggcatca gcgctgaaaa caaaaataaa
      121 tccggatatt atgaaacagt ataacctttt gcggaaaaga aatcttgaga ataaccttaa
      181 agcagttgag cttgtttcaa atggaagtat tgatttctta cttcttcccc aggaggattg
      241 tttcccaaac gggccacaga cccaggaaca gctgaccata tctgagaaga tactttcact
      301 taacttatca gagaaggttt tccttcataa cggagccgat gaagcagctc aggagcttgt
      361 gtcaaaagtt gcaaatccag gagaaaaaaa aatatcggtc ctgtgtgact gtccttcaaa
      421 actcaatgaa ataatggagt tcgaagacag accatttcag accaatcttt tttcacacat
      481 gaaagcactt aactttatac aagataatga ttctcaaact ctcctttaca tagcctttgc
      541 cgaaccacag acctgtgtgc gccatataaa aactgccatt gcttacggga aaaaagtcat
      601 ccttttggat ctgatagctc caaatggcgg cagtaaagaa cttataagcg aaatgatcag
      661 acatgatctg cttaggcaac tctggggata cagtgcttgg aacaccgcaa gcaactctct
      721 gggaacagca ctggcaatct gtgctcttac caatatgaag acagaaagaa aaaaaatatc
      781 ggagcttctt gcaagaaatc ttgtggatga cttcctctat caaagaatct acagacaggt
      841 tattgacgaa agattcaaaa aagatattca caacatgcag acagttgaca aaaaaaacag
      901 agaggatatt aaaaaagaat ttatttcatg tgcaaataaa tttctcaatg aaagcggttt
      961 caacggactt aaaattacag atttctactt cccttgggaa agaacttttg agtgcggtgt
     1021 cacgtgttct taccaataaa acatctttta caatcttttt cgacgtaatc tgatacgctc
     1081 tgaagatatc gtttttcaaa ggattaaaag aaaaccacaa aaataagaaa gcccaaacag
     1141 ctactattgc tatacttatt ttatatactg tgtctgaaaa cttcctgcag cggaatattc
     1201 tataccccag catttgccct aaagcaaccg aaataataac aataaaaata tccatagctt
     1261 ggatatgtcc ggaaaaactg gtatatagat aaaacccaga tattatgaaa acaaccataa
     1321 aagttgccgc aaaaactttt gctgcaaaaa aattgttagt ttttttcttt ataaaaatac
     1381 tttctaccaa tccccaaaaa agcgtcggcc agaatacaag cttaagctgc tcccaaatac
     1441 tttcatttaa agcaaaaaat aaagtcagaa gaaaaaaatt tccggcataa ctgaatatat
     1501 aatgaaaaag tactcctata aggcaaacga agaaaaatcc tccgacttcc cagtaaaaaa
     1561 gagttttttt gtctttctct ttatacatat gatcagctcc cgaagtttta tatacatttt
     1621 attaatcaaa tatattatat attaatatta tgtcaaaaac aaaacatcgt tatatactcc
     1681 aaaaatcata attgccttca tgattttgga gtgacttcag tattgtttgt gcactttcca
     1741 ggttggcaag cgcaatatca agactgtact ttactgcgta aaaaacgttt tctggaagtt
     1801 tttgtttgga aatttcctca tactgtctgc ttagatggga tgtatacttc tgcagatata
     1861 aacaacgcag ttctataatg cggctgaact cttgcctgcc tataaccggc ataaaaaaaa
     1921 ttcttattgt a
//
LOCUS       DAOSHR010000323         1918 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_60395_length_1918_cov_3.234568, whole genome shotgun sequence.
ACCESSION   DAOSHR010000323 DAOSHR010000000
VERSION     DAOSHR010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60395_length_1918_cov_3.234568"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1057)
                     /locus_tag="PLU28_11215"
     CDS             complement(<1..1057)
                     /locus_tag="PLU28_11215"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOG35671.1"
                     /translation="MKKIRLLLVVIMIMLPVFIFSAKVVVRVPQDPDFLDPHKAAASG
                     TEEMMFNVFEGLLKADSNGNVVPAVAERYSVSPDGLVYTFKLRKGIKFHNGALVTVDD
                     IIYSLDSLRGANNEKPLSSTFSSFIKDVKATDKETVVVSMKELNTSFITNFTVAIIPK
                     NNVDPNKNPIGTGPFKFKEYLPGQRVVIQKFSDYWKKGVPTIDEVEFRIITDDQAALL
                     ALLSGEIDMSPRIDVLQLDLLSDNFQVIKSEQNMVQLLALNHQNKYFSDVRVRQAINY
                     AVDKDEIIDAVALGYGTKLGSNMSPVMKKYYRQGLENYYQKDIAKAKELLRQAGYPNG
                     FRTTITVPSNYKFHVDTA"
     gene            complement(1080..1811)
                     /locus_tag="PLU28_11220"
     CDS             complement(1080..1811)
                     /locus_tag="PLU28_11220"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOG35672.1"
                     /translation="MRLDIIGCCGAYPGNGKPCSGYLITQNETSVLLDCGSGVLTKIY
                     EYIDPNRINHIIITHFHADHFSDIFCIQHLSLVAMQLGRRKEPLRIYAPADDEYFEKM
                     KYEQASVAFEINSLNTLDIDSIHVSFLKNSHVKSAYAVKVLSGQRSLVYTSDTQWCDE
                     LVDFAGDCDVLLCESSLYNEFYNVVKGHMTAGECGLLAAKCRAGRLVLTHLPHYGDHK
                     VLAVQAREKYNGPVILAENIRYLDI"
BASE COUNT          554 a          398 c          341 g          625 t
ORIGIN      
        1 gagctgtatc aacatggaac ttatagtttg aaggaactgt tatagttgtc ctgaatccgt
       61 ttggataacc tgcctgtctg agaagctcct ttgcttttgc tatgtctttc tggtagtaat
      121 tctctagtcc ctggcgatag tattttttca taacagggct catattggaa ccgagcttgg
      181 taccatatcc caatgcaact gcatctatta tttcatcctt gtctacagca tagtttatag
      241 cttgtctcac cctcacatcg gaaaagtatt tgttctgatg attgagcgct agtagctgaa
      301 ccatattctg ttcggacttt attacctgaa agttgtcaga aagaagatca agctgaagta
      361 catctattct gggagacatg tctatttcac cagaaagcaa tgccagtaag gcagcctggt
      421 catccgttat tatcctgaac tctacttcat ctatcgtggg aacacctttt ttccaataat
      481 cagagaattt ttggatgact actctctgtc cgggaagata ttccttaaat ttaaaaggac
      541 ctgtgcctat aggattttta ttcggatcga cattattttt gggaattata gcaacagtga
      601 agttggttat aaaggatgtg ttcagttctt tcatactgac cacaaccgtt tctttatccg
      661 tagcctttac atcttttata aagctgctga aagtagaaga aagaggtttt tcgttgtttg
      721 cgccgcgcag actgtcaaga gagtagatta tatcatcaac agtcacaagt gctccgttgt
      781 ggaattttat tccttttctg agcttgaaag tatacacaag tccgtcagga gaaacactgt
      841 atctttccgc aacagcaggc actacatttc cgttggaatc tgccttcaaa agcccttcaa
      901 aaacattgaa catcatttct tcggttcctg aggcagcggc tttgtgggga tcaagaaagt
      961 caggatcctg aggaactctt accactactt tggctgaaaa tataaaaaca ggaagcatta
     1021 tcataataac tactaataga agtcttattt ttttcatatg atccctccta ataaagaatt
     1081 tatatatcta aatatcttat attctctgca agaatgacag gcccattata cttttcacgt
     1141 gcctgtacag ccagaacctt atgatctccg taatgcggaa gatgagtaag aactaatctg
     1201 cctgctctgc attttgctgc tagcagtccg cattcaccgg ctgtcatgtg acctttaaca
     1261 acattgtaaa attcattgta taatgaactc tcacacagaa ggacatcgca atctcccgca
     1321 aagtcaacca gttcgtcaca ccactgtgta tcagaagtat aaaccaatga gcgttgaccg
     1381 cttaaaactt ttacagcata agcgctttta acatggctgt tcttgagaaa cgagacatga
     1441 attgaatcta tatccaacgt gttaagagaa ttgatttcaa aagcaacaga tgcctgttca
     1501 tacttcattt tttcaaagta ctcatcatcg gcaggagcat atattcttaa cggttctttt
     1561 ctccttccca gctgcattgc cacaagacta agatgctgta tgcagaaaat atccgaaaaa
     1621 tgatctgcat gaaaatgcgt tatgataatg tgatttattc tgtttgggtc aatatactca
     1681 tatatttttg tcagcactcc actgccacag tccagaagaa cagatgtttc attctgtgta
     1741 atcagatatc ccgagcatgg tttaccattg ccgggataag ctccgcaaca gcctattata
     1801 tccagcctca ttaatatcag tccgcctata gttaagcatg tagcacttca ttattttctg
     1861 tctatattat atactattct tttttttata actttaacat atattaagat ttatttca
//
LOCUS       DAOSHR010000324         1915 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_60507_length_1915_cov_4.808065, whole genome shotgun sequence.
ACCESSION   DAOSHR010000324 DAOSHR010000000
VERSION     DAOSHR010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60507_length_1915_cov_4.808065"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1115
                     /locus_tag="PLU28_11225"
     CDS             <1..1115
                     /locus_tag="PLU28_11225"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOG35673.1"
                     /translation="PDFVYHPFLTSHSQREVTVNFKLNMPVLAYVLLYENGVLKEKSE
                     EAPLSDLYHINFKNFDPKSSYTYEAVAENFHGEITLKSGRLELKNPADATDDYSFVVY
                     GDTQKYVNMHSRVVQRMYAENPSFVIHCGDLVEKGDSTQDWIDFFKSMRPLGQKVPYY
                     TVLGNHEYNTFNYYSALSLPKGGGNYEKRWYSFEYGDIFFIMLDSNILTGDPNFAAQT
                     AWLEDQLKNSQNKRYRFLVFHHPFWTTSQEYGPMKENLAQGHELTEFWFDLFIKYNVQ
                     AVFNGHIHGYERYFKDGIMFVTSGGSGGPLDTKHTAEKLPWSKVDVTGHWNYVLAEVS
                     PKGILFTVKGVAKSLKPLDENAFEEEDIILDRFFIQ"
     gene            complement(1109..1759)
                     /locus_tag="PLU28_11230"
     CDS             complement(1109..1759)
                     /locus_tag="PLU28_11230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35674.1"
                     /translation="MKRAVTVALIVNIFLIVLSQKPEIMEYTEEINGKRTILTISIIT
                     SENGRVIAQKQSENEYYKIINMNTFTTEFSYENKVTGLSYDAVRNGYVIQVAGKDQNK
                     KIIKNFKIDDCGWEQSIELSLAQLVLSGNKDKRFWLVSLDGERCMKMYAVNKGMEELQ
                     INGKKVNAIKIEVTPDGFFSIFWKAFFWYDQNGLYLKSRCSKDFGAPECITEIYNY"
BASE COUNT          566 a          386 c          340 g          623 t
ORIGIN      
        1 atcccgactt tgtttaccac cctttcctca cgagtcacag tcagcgagag gtaaccgtaa
       61 acttcaagct caacatgcct gttctggcat atgtgctact gtacgaaaat ggtgttctta
      121 aagaaaagtc tgaagaggca cctttaagcg acttgtacca tatcaatttc aagaattttg
      181 atcctaaaag ctcctatact tatgaagctg ttgcagaaaa ctttcatgga gagataactc
      241 ttaaaagcgg caggttagaa cttaaaaatc ctgcggatgc tactgatgac tactcttttg
      301 ttgtatacgg agatacacag aagtatgtga atatgcacag cagagtagta cagagaatgt
      361 acgccgaaaa tccatccttc gtgatacact gtggagatct tgtagaaaaa ggtgactcaa
      421 cacaggactg gattgacttt tttaagagca tgcgccctct tggtcagaaa gttccatact
      481 atactgttct tggaaatcac gaatacaata cttttaatta ctactctgcc ctttcactac
      541 caaaaggagg aggaaactat gaaaaacgct ggtattcttt tgaatatgga gacatattct
      601 ttataatgct tgattcaaat attcttacag gagatcctaa ctttgccgct cagacagcat
      661 ggcttgagga tcaacttaag aactcacaaa ataaaagata ccgatttttg gtatttcatc
      721 atccgttctg gacaacttcc caggagtacg gcccaatgaa ggaaaatctt gcgcaggggc
      781 atgaacttac tgaattttgg tttgaccttt tcataaaata taatgttcag gctgttttta
      841 acggtcatat acatggttat gaacgatatt tcaaggacgg cataatgttt gttacctccg
      901 gtggcagtgg aggtcctctt gatacaaagc atacggcaga aaaacttccc tggagtaaag
      961 tcgacgttac aggtcactgg aactatgttt tagctgaagt ttcacctaaa gggatactat
     1021 tcaccgtaaa aggagtggcc aaatccttaa aacctcttga tgaaaatgct tttgaagagg
     1081 aagacattat tcttgacaga ttttttattc agtagttgta aatttctgta atacactccg
     1141 gtgctccaaa gtctttggag caccgtgact ttaaatatag tccgttttga tcataccaga
     1201 aaaaagcttt ccagaaaatg gaaaaaaaac catccggagt cacttcaatc tttatggcat
     1261 ttactttttt cccatttatt tgtagttctt ccattccttt atttacagca tacattttca
     1321 tgcatctctc tccatcaagg gataccaacc aaaatctttt atctttattt ccggaaagta
     1381 ccaactgagc aagcgaaagc tctattgact gctcccatcc gcagtcatca attttaaagt
     1441 ttttaatgat ttttttgttt tgatcttttc cggctacctg aattacataa ccgtttctaa
     1501 cagcatcata cgacagtccg gttactttat tttcataact gaactcggtt gtgaaggtat
     1561 tcatgttaat tatcttatag tactcatttt cactttgttt ctgagcaata accctgccat
     1621 tctcgcttgt aataatgctt atggtaagta tggttctttt cccgtttatt tcttcggtat
     1681 actccattat ttcaggtttc tgagaaagaa ctattaaaaa tatattaact atcagagcta
     1741 cagttaccgc ccttttcatt tcagcccttc ctgttctgcc tgaaccagcc tacagaatct
     1801 tttatactct gttcaaggct tcttggagaa aaacccagct gtctccttgc tttaaaatta
     1861 cttatattac tgttgctgtt cagagtatat aacgaatatg gtgtaagaag agctt
//
LOCUS       DAOSHR010000325         1912 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_60654_length_1912_cov_5.469575, whole genome shotgun sequence.
ACCESSION   DAOSHR010000325 DAOSHR010000000
VERSION     DAOSHR010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60654_length_1912_cov_5.469575"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..1583
                     /locus_tag="PLU28_11235"
     CDS             114..1583
                     /locus_tag="PLU28_11235"
                     /inference="COORDINATES: protein motif:HMM:NF012470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HOG35675.1"
                     /translation="MNKKILLVLALSVIASMTYASVKYLFLFIGDGMSIPQINSAELY
                     LTALNGGSTVKKLTMSTFPAQGVTTTYDADSFIPDSASAGTSIACGVKTHSGVVGMDI
                     TRNNPVKSIAEIAKEKGMKVGILTSVSLDHATPAVFYAHQASRSSYYDISVDLVKSGF
                     DYFGGGGFLQPKGKKNDKTDIYELAAKMGYKVVKTSADMKKLSSSDGKVIVVNEVLAP
                     SQALQYELDRQSSPSLADFTENAIKMLDNPNGFFIMVEGGKIDWSCHANDAASTINDV
                     IAFDSAIKKAVEFYSRHPAETLIIVTGDHETGGMTIGFAGTGYSTYLDNIQNQKVSYE
                     SFDSIFGSLKQSSSNLSFKDVLPLIKDYFGLCILTEKEKTEYTALSNSGSKEASDKLR
                     MNLSGSDLKKLEDAFNMSMKKSSERPNDDDTYLLYGGYEPLSVTLTHILNQKCGISWT
                     TYSHTGLPVTTFALGNCQELFNGYYDNTEIFKKMVYALN"
     gene            1593..>1912
                     /locus_tag="PLU28_11240"
     CDS             1593..>1912
                     /locus_tag="PLU28_11240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35676.1"
                     /translation="MLKRIAKMDLLFVAFLILATAAMLLIPSSSELKKNYSYYKARIV
                     ETDNSLVGQYGLIRQGTQELKLELLNGPYKGKRFETSNNLIGKMELDKFLKNGDIVLA
                     EVFET"
BASE COUNT          605 a          354 c          392 g          561 t
ORIGIN      
        1 ttgcatatat gaaatatatt aacttttatt attatcactt aataatactt agaacttgcc
       61 aaaaaattaa tttgtaaact ttcaatgtaa ttaattctct atggaggtgt ccaatgaata
      121 aaaagatatt gttggttttg gcgttgtcag ttattgcaag tatgacatac gcatcggtaa
      181 agtatctttt cctgtttata ggtgatggta tgtccatacc gcagataaac tcagccgagc
      241 tttaccttac tgctcttaat ggtggttcaa ccgtaaaaaa gcttaccatg agcacctttc
      301 cggcacaggg cgtaactaca acttatgatg ctgactcttt tatacccgac tcggcatcgg
      361 caggaacctc tatagcctgc ggagtaaaga cacattccgg agtagtagga atggatataa
      421 ccagaaataa tccggtaaaa tcaatagcag aaattgctaa ggaaaaagga atgaaggtcg
      481 gtatcctgac aagtgtttca cttgaccatg ccactccggc agttttttat gcccaccagg
      541 cctcaagaag ctcttactac gatatatcag tcgatcttgt aaaaagcggc ttcgattatt
      601 tcggcggcgg cggctttctc cagcctaaag gaaagaaaaa tgacaagact gatatatacg
      661 aacttgcagc taaaatgggc tataaagttg ttaaaacctc cgctgatatg aaaaagcttt
      721 catcttcaga cggaaaagtc atagttgtaa atgaagttct tgcaccgagt caggctttac
      781 agtatgaact tgacagacag tcatctccct ctttagccga ttttaccgag aatgctataa
      841 aaatgcttga taatccaaat ggttttttca taatggttga aggtggtaaa attgactggt
      901 catgtcatgc caacgatgcg gcatcaacta taaacgatgt aattgcattt gatagcgcaa
      961 ttaaaaaggc cgttgagttt tacagcagac atcctgcaga aacgcttata atcgttaccg
     1021 gagaccatga aaccggcggt atgaccatag gttttgcggg aacgggttat tcgacctatc
     1081 ttgataatat tcagaatcag aaagtttctt atgaaagttt tgacagtatt tttggctctt
     1141 tgaagcagtc ttcatcaaat ttaagtttta aagatgttct tcctctcata aaggattatt
     1201 tcggtctgtg tatccttacc gaaaaggaaa aaactgagta tacggcactt agcaattctg
     1261 gaagtaaaga agcttctgat aagttaagaa tgaatctttc cggtagcgac cttaaaaagt
     1321 tggaagatgc tttcaatatg tctatgaaga agtcttccga aaggccaaac gatgatgata
     1381 cctatcttct ttacggcgga tatgaaccgc tttcagtgac attaacccat atactcaatc
     1441 agaagtgtgg aatatcttgg actacatact cacataccgg gttaccggtg acaacctttg
     1501 cccttggaaa ctgtcaggag ctttttaacg gatactacga taacaccgag atattcaaaa
     1561 aaatggttta tgcgctgaac tgatgagggt gtatgcttaa aagaattgca aagatggatc
     1621 tgctgttcgt ggcttttttg atacttgcca ctgctgcaat gttgcttatt ccatcttcat
     1681 cagaacttaa aaagaattac tcttattaca aggcaagaat cgtagaaact gacaacagtc
     1741 tggtaggtca gtacggactt atacgccagg gcactcagga gcttaagctt gaactgctga
     1801 atggacctta caagggaaag aggtttgaaa cttcaaacaa tcttataggc aagatggaac
     1861 ttgataagtt ccttaaaaat ggagatatag tacttgcgga agtatttgaa ac
//
LOCUS       DAOSHR010000326         1907 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_60886_length_1907_cov_3.851512, whole genome shotgun sequence.
ACCESSION   DAOSHR010000326 DAOSHR010000000
VERSION     DAOSHR010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60886_length_1907_cov_3.851512"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1156)
                     /locus_tag="PLU28_11245"
     CDS             complement(<1..1156)
                     /locus_tag="PLU28_11245"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOG35677.1"
                     /translation="MQIIKVTNIKELEELISKVTQAQKAYSSYTQEKVNEIFRRAAIS
                     ANAYRIWLAKSAVEETGMGIVEDKVIKNHFAAEYIYNKYKDESTCGIIEEDKAFGIKK
                     IAEPIGVIAGIIPTTNPTSTTIFKSLLALKTRNAIIFSPHPRAKKSTVAAARVILEAA
                     IACGAPENIIGWIDEPSVELSQYLMKHKNINLILATGGPGMVRAAYSSGKPAIGVGAG
                     NTPAIIDETAHLKMAVNSILMSKTFDNGVICASEQSVTVLSEKYDEVKKEFIDRGAYI
                     MNADEKEKLGKILIHNGNINPDIVGQPACKIAGLAGFNVDKSTKVLIACCEKIGDEEP
                     FSHEKLSPVLALYRADSFEDAVQNAVSLVEFGGMGHTSVLYTNQKNHERIS"
     gene            1407..1784
                     /locus_tag="PLU28_11250"
     CDS             1407..1784
                     /locus_tag="PLU28_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 domain-containing protein"
                     /protein_id="HOG35678.1"
                     /translation="MVIKRKISSCPVCNGQLMITRYRCENCGTEISGSFPLEEFAKLT
                     DEQLTFLKIFIKTRGNLSELQKEMDISYPTAKARLEDLARAMGYEAEDDNRTKTFEIL
                     EKIEKGEITPEEAKELLKKTKSK"
BASE COUNT          546 a          354 c          360 g          647 t
ORIGIN      
        1 aacttatcct ttcatggttt ttctgattcg tatataacac agaggtgtga cccatgcctc
       61 caaactctac aagactgacg gcgttttgga ctgcatcttc aaaagaatcg gctctgtata
      121 aggcaagaac cggtgaaagc ttttcatgag agaaaggttc ttcatcaccg attttttcac
      181 agcaggctat aaggacttta gtgcttttat caacattgaa gcctgcaagt ccggcaattt
      241 tacaggctgg ctggcctaca atatccggat tgatattccc gttatgtata aggatttttc
      301 cgagtttttc tttttcatct gcattcatga tatatgcgcc tctgtcaata aactcctttt
      361 tcacttcgtc atatttttcg cttaaaactg tcacagactg ttcagaagca catattacac
      421 cgttgtcaaa ggttttgctc ataagaatgg agtttaccgc cattttcaga tgagccgttt
      481 catctattat agccggagta tttcctgccc cgacaccgat tgcaggtttg ccgcttgaat
      541 aggctgctct taccatgcct ggcccaccgg ttgcaagaat cagatttatg tttttatgtt
      601 tcatgaggta ctgagaaagc tctacactcg gttcatctat ccatccgatg atgttttcag
      661 gtgctccgca tgcaatggct gcttctaata tcacacgtgc tgcagctaca gttgattttt
      721 ttgctctggg atgaggagaa aatatgattg catttcttgt tttcaaagct aaaagtgatt
      781 taaaaatagt tgtggatgtc gggttggttg ttggtataat gccggctatt acacctatgg
      841 gttcggctat cttttttatt ccgaatgcct tatcttcttc tattattcca caggtgcttt
      901 catctttata cttattgtag atgtattctg ccgcaaaatg gtttttaatg actttatctt
      961 caactattcc catacctgtt tcttccacgg cgctcttggc aagccatatc ctgtaagcat
     1021 tagctgatat agcggctctt ctgaagatct cattaacttt ttcctgtgtg taagaggaat
     1081 aagctttctg tgcctgtgtt actttagaaa taagctcttc aagctccttg atattggtta
     1141 cctttattat ctgcataatt atgcctcctt cttgaatgac tttgtttttt ctttcacttg
     1201 aattttacgc tatatatatt actctcccgc atgttataat gtaaattgat agttaaaaca
     1261 ttcacaaatt aacttttaaa aagctctctt taattttttt agagaataaa taaatttttt
     1321 tattattttt ttctgtgaaa acttgacttt tttaaaaaaa tatattataa ttttgatatc
     1381 ctagaaaatt agaaggagga gtagacatgg tgataaaaag aaaaatctca agttgtcctg
     1441 tctgtaatgg tcagcttatg ataacaagat accgttgtga aaactgcgga actgagattt
     1501 caggaagttt cccgcttgaa gagtttgcca agcttacaga cgaacagctc acttttctga
     1561 agatatttat aaagacccgt ggaaatcttt ctgaactcca aaaagagatg gatatatctt
     1621 accctaccgc aaaggcaaga ctggaagatc ttgcacgggc tatgggatat gaggcggaag
     1681 acgataacag aaccaaaacc tttgaaatac tcgaaaaaat agaaaaaggt gagataactc
     1741 ctgaggaagc caaagagctt ctcaaaaaaa ctaaatcaaa gtaggagggg agctcaatgc
     1801 agggatttat tacttttgaa gataatatca ggaaagttgt tatttcagca aaatttatgg
     1861 gaatggatat aagcgtgaat gaatctgagc agaatacttt tgagtat
//
LOCUS       DAOSHR010000327         1890 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_61766_length_1890_cov_2.964578, whole genome shotgun sequence.
ACCESSION   DAOSHR010000327 DAOSHR010000000
VERSION     DAOSHR010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61766_length_1890_cov_2.964578"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(141..929)
                     /locus_tag="PLU28_11255"
     CDS             complement(141..929)
                     /locus_tag="PLU28_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OAM dimerization domain-containing protein"
                     /protein_id="HOG35679.1"
                     /translation="MSGGLYSLDHKDFDKNLDLKSVRPYGDTMNDGKVQLSFTLPVPC
                     GDEALQAAKDVIRSMGLENPQIVYFKELAPGFTFFNAYGECTKTIDFTSIHVAKADVK
                     KMGMKDIDEYIKQTLNRKIVVVGASTGTDAHTVGIDAIMNMKGFAGHYGLERYEMFEA
                     YNLGSQVLNEDFIAKAIELKADALLVSQTVTQKNVHIKNMTELVEMLEAEGIRSKVVL
                     VAGGARITHELAKEIGFDAGFGPNSFAEDVGAFIAQEMARRLGK"
     gene            complement(929..>1890)
                     /locus_tag="PLU28_11260"
     CDS             complement(929..>1890)
                     /locus_tag="PLU28_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysine 5,6-aminomutase subunit alpha"
                     /protein_id="HOG35680.1"
                     /translation="TEGFGGTFATQENFRIMRKALDEVAHETGKYVRLCNYCSGLCMP
                     EIAAMGALERLDVMLNDALYGILFRDINMKRTIIDQFFSRVINGFAGVIINTGEDNYL
                     TTADAYEEAHTVLTSQFINEQLALVAGIKPEQMGLGHAFEMDPSIENGFLYELAQAQM
                     AREIFPDAPLKYMPPTKFMTGNIFNGYIQDTLFNIVSIWTKQGIQLLGMLTEAIHTPF
                     MSDRYLAIENAQYIFNNLKNIGDEVTFKDGGIIQTRAGAVLDRSVELLEKIEKEGLFN
                     SLEKGVFANVKRPINGGKGGDGVFEKGKYYFNPFIEKMLKEGL"
BASE COUNT          499 a          445 c          343 g          603 t
ORIGIN      
        1 ggttccgagt gagacatcca ggattcttgc aatgaagatt acaataggca taactaccca
       61 tttgaaaatg tctggtgaca gtagatcatt caaaacactt ccctccaatc agttcagata
      121 agtagattat accataaaaa tcatttgcct agacgtcttg ccatttcctg ggctataaat
      181 gcacctacat cctctgcaaa ggagttgggg ccaaagcctg catcaaaacc gatctctttg
      241 gccagctcat gagttattct ggcacctccg gcaacaagaa ccactttgct ccttatacct
      301 tcggcttcaa gcatttctac aagttcggtc atgtttttta tatgcacgtt tttctgggtt
      361 acggtctgag atacaagcag ggcatctgct ttaagctcta tcgcttttgc aataaaatct
      421 tcgtttaaaa cctgactccc aagattatat gcttcaaaca tctcgtatct ttcaagtccg
      481 taatgtccgg caaagccttt catattcatt atagcatcta ttccgactgt gtgtgcgtca
      541 gtgcctgtac ttgcacctac caccacgatt tttctgttta aagtctgttt tatgtactca
      601 tcaatatcct tcatgcccat cttcttcaca tcagcttttg caacatgaat tgaagtgaag
      661 tctatcgttt tggtgcattc accgtaggca ttgaaaaagg tgaagcccgg tgccagttcc
      721 ttaaaatata caatctgagg gttctcaagc cccatgcttc tgattacatc ctttgcagcc
      781 tgcagagcct cgtcaccgca cggtacggga agggtaaaac ttaactgtac cttaccgtcg
      841 ttcatggtgt ctccgtaagg tcttacggac ttaagatcaa gatttttgtc aaaatccttg
      901 tgatcaaggg agtatagtcc accgctcatc acaatccctc cttaagcatc ttttcaatga
      961 atggattaaa gtaatacttg cctttttcaa atactccgtc tccgccttta ccgccattta
     1021 taggcctttt tacatttgca aaaacacctt tttcaagaga gttaaagaga ccttcttttt
     1081 ctattttttc aagcagttcg actgacctgt caagtacagc tccggctctt gtctgaatta
     1141 tacctccgtc tttaaaggta acctcatcac ctatgttttt gaggttgttg aatatatact
     1201 gtgcattttc aatggccaga tatctgtcag acataaaagg agtatgtatg gcttctgtca
     1261 gcatccccaa aagctgtatt ccctgttttg tccatataga tacgatatta aaaagagtat
     1321 cctgtatgta accattgaaa atatttcctg tcatgaactt ggttggtggc atatacttaa
     1381 gcggtgcatc ggggaatatt tcccttgcca tctgagcctg tgcaagctcg tataaaaagc
     1441 cgttttcaat agaaggatcc atttcaaatg catgtcccag acccatctgt tcaggtttta
     1501 ttccggctac cagggcaagc tgttcgttta taaactggga ggttaaaacg gtgtgagctt
     1561 cttcatatgc atctgctgtt gtcagatagt tgtcttcccc ggtatttatg attacacctg
     1621 caaatccgtt tattaccctt gagaagaact gatctataat agttctcttc atatttatat
     1681 ccctgaaaag aattccgtaa agcgcatcat tcagcataac atcaagccgt tcaagagctc
     1741 ccatggcagc aatttcaggc atacacagtc ccgaacagta gttgcaaaga cgtacgtact
     1801 tacccgtttc atgtgcaacc tcatccaaag ctttccgcat tattctgaag ttttcctgtg
     1861 tggcaaatgt tcctccaaag ccctctgttg
//
LOCUS       DAOSHR010000328         1875 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_62552_length_1875_cov_5.412088, whole genome shotgun sequence.
ACCESSION   DAOSHR010000328 DAOSHR010000000
VERSION     DAOSHR010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1875
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62552_length_1875_cov_5.412088"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="PLU28_11265"
     CDS             complement(<1..260)
                     /locus_tag="PLU28_11265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35681.1"
                     /translation="MKKLLIVLDFSLILLFNLFLTKNILASAIMAVSLIIGFYSFGIY
                     TSSVIKSFNTSLVRMVSGIMIAFFVVFIFIFVFRLPINRFHFL"
     gene            complement(278..1666)
                     /locus_tag="PLU28_11270"
     CDS             complement(278..1666)
                     /locus_tag="PLU28_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOG35682.1"
                     /translation="MRKLCKVLDFALLFFFNLLLGFSLLSSFIISFIIFLGLYSFRVY
                     DQENFSGLNEAVLRVLAGSCAGIFSVMIVYVFFADSLIDKASPFFLNLFFTTVFFPFI
                     HKFEHTIYLKRTRPKNYLVIGRKAEVGKILDEITERSFGKLRFIEYINPSPEKLQEIV
                     ENDFYAQIKKAIKELDPPQVDRSFEETAKALVREHIVEGDSQHMLDAILVTDPKLESL
                     VKSELLNYQKNQIEVDYLPIIAEKYLKRVPLVVAEKFKSYYEVSFQDYKYSPAKRAID
                     LLFSILFLVLFSPIIAVCGLWILIENGMPVIFKQHRVGQGQKYFLMHKLRTLKNTSNE
                     IISEKDFTLQNDTDKRVLAIGKFLRKFRLDESLQFYDVIKGTMSIVGPRPEMKEFNDE
                     MKDKIPFYLNRLKAKPGITGWAQINYKYTTTLEEYRTKTEYDLYYIKNRTTLLDLQIM
                     LKTIETMLKIRK"
BASE COUNT          643 a          336 c          295 g          601 t
ORIGIN      
        1 agaaaatgga atctgtttat cggaagtctg aaaacaaata taaaaataaa aactacaaaa
       61 aacgcaatca ttattccgga aaccatacgc accaatgatg tgttaaagct tttaattacc
      121 gatgatgtgt atattccaaa agaataaaag cctataatca agcttacagc cataatagct
      181 gaagctaaaa tattttttgt gaggaaaaga ttgaataaaa gaataagaga aaaatcaagc
      241 acaataagaa gttttttcat aagccacccc gcttcattta ctttcgtatc ttgagcatag
      301 tttcaatagt tttcagcatt atctgtagat ccagcagagt cgttctgttt tttatgtagt
      361 aaagatcgta ttctgtcttg gttctgtatt cctctaaagt agtcgtatac ttatagttta
      421 tctgtgccca tccggttatt ccaggcttgg ctttaagtct atttagatag aatggaattt
      481 tatctttcat ttcatcatta aactctttca tttccggacg gggcccgact atactcattg
      541 ttccctttat cacgtcataa aactgaaggc tttcatccaa tcggaattta cgcagaaact
      601 tacctatcgc aagtaccctt ttgtctgtat cgttctgcag agtaaagtct ttttctgaaa
      661 tgatttcatt tgaggtattc tttagtgtcc tcagtttgtg cataagaaag tacttctgtc
      721 cctgacctac cctatgctgc ttaaaaatga ccggcattcc gttttctata agaatccata
      781 aaccgcacac cgctattata ggcgaaaata aaacaagaaa tagtatggag aatagcaaat
      841 caatcgcccg tttggcaggc gagtatttat aatcctgaaa acttacttca tagtaacttt
      901 taaacttctc ggcaacaaca aggggaactc ttttaaggta cttttcagca attattggta
      961 gataatcaac ttctatctga ttcttctggt agttaagcag ttctgatttt acaagacttt
     1021 caagctttgg atctgttaca agtattgcat ccagcatgtg ctggctgtcg ccttctacga
     1081 tatgctccct aaccagagcc ttggctgttt cctcaaatga tctgtctact tgaggtgggt
     1141 caagttcttt tatggctttt tttatctgtg cataaaaatc attttcaaca atctcctgaa
     1201 gcttctccgg agaaggatta atatactcaa taaatcttag ttttccaaag gatctttcgg
     1261 ttatttcatc aagtatcttt cctacctctg cttttcttcc tattacaagg tagtttttag
     1321 gtctggttct ttttaagtat atagtgtgtt caaacttatg tataaagggg aagaagactg
     1381 tcgtaaagaa aagattgagg aaaaaaggag aagctttgtc tataagactg tctgcaaaaa
     1441 aaacataaac aatcataacc gaaaagattc ctgcacatga tccggccagt actctcaata
     1501 ctgcctcgtt taagcctgaa aaattttcct ggtcataaac tctgaatgaa taaagaccaa
     1561 ggaagattat aaaagatatt ataaaagaag aaagaagact gaatcccaaa agaagattga
     1621 aaaaaaacaa aagggcaaaa tccaatacct tgcataattt tctcaaatcc gcacctcaag
     1681 taaaaaataa aaagtaaaaa tatcaacata taaaaatatc aacataatag tattttatca
     1741 cagttatatc aaatactatt ggtaaaaatg ttttataaag ataaaccgta taaaatcaag
     1801 gataacctgt aattgaaaaa tataaagatt caatagtttc acaaatattt tatcacacaa
     1861 taactctttt gcaaa
//
LOCUS       DAOSHR010000329         1816 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_65677_length_1816_cov_3.202726, whole genome shotgun sequence.
ACCESSION   DAOSHR010000329 DAOSHR010000000
VERSION     DAOSHR010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65677_length_1816_cov_3.202726"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="PLU28_11275"
     CDS             <1..231
                     /locus_tag="PLU28_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur binding hydrogenase"
                     /protein_id="HOG35683.1"
                     /translation="SEVMRTAKPDSVWITYQTHQNIIAVAEVVGIKAIIIPCGLHYSE
                     DTAEKACEHGIYLLESDESAFAVSGKLYGLIS"
     gene            233..958
                     /locus_tag="PLU28_11280"
     CDS             233..958
                     /locus_tag="PLU28_11280"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOG35684.1"
                     /translation="MALFNASFHNHSVLSPCADITMTPEVYAQKLTYAGIDWIALTDH
                     NSCRNVRIFERLLSEHGIQVLPGIEVQTKEEIHILFYFSSFKELEEFSMYIEERVIIK
                     DYDPEKLGYQIELNEEGEFNNILSYPYLGSACDLSLEDAVRAARKYDSLCVPAHIFRS
                     FGLITQLGIPPEIEFDGVEVKNPREYETAKKLGFSRFLFGMDAHTPDELEGIACTVET
                     EKRDFEHLKTALYKGKVFPLWQH"
     gene            946..1488
                     /locus_tag="PLU28_11285"
     CDS             946..1488
                     /locus_tag="PLU28_11285"
                     /inference="COORDINATES: protein motif:HMM:NF024977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG35685.1"
                     /translation="MATLKTIADHIMDICENAVRSKSKLCELYIDEYETSFFFRISDK
                     GIGMDEQEVKKALDPFYTTKTERRKKFGMGLPFLKFSSELTGGNFKITSEKGYGTVVE
                     VLFKKDSIDCQPVGDIAQAIFSVVYLDHDTDWYIKRTKNSEAYEINSSDMKKEYGETF
                     FSPSFMKNIKDLIISLENEF"
     gene            1549..>1816
                     /locus_tag="PLU28_11290"
     CDS             1549..>1816
                     /locus_tag="PLU28_11290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35686.1"
                     /translation="MTKKIFLVAVLCLSVLTAFSITKLQKIEYSENISYAASEISRIF
                     ENYEIKEGNMIGEIDVKLAIKQIMELGYFTSMDFELNNDTGVLLF"
BASE COUNT          607 a          297 c          386 g          526 t
ORIGIN      
        1 agcgaggtca tgcgtaccgc aaaaccagac tctgtttgga ttacatatca gactcatcaa
       61 aatattattg ctgtagcaga agttgtcgga ataaaagcaa taattattcc ttgcgggctg
      121 cattactctg aagataccgc tgaaaaagcc tgtgagcatg gaatatatct tttggaatcg
      181 gacgaatcag cttttgctgt gagtggaaag ttgtacggat taataagttg atatggccct
      241 tttcaatgca tcttttcata atcactctgt tctttcacca tgcgcagata taaccatgac
      301 accggaagtg tatgcccaaa agctcacgta tgctgggata gactggatag cacttaccga
      361 tcataactca tgcaggaatg tacggatctt tgaacgactg ctttctgagc acggaataca
      421 ggtgcttcca ggaatagagg ttcagactaa ggaagaaatc cacattcttt tttatttcag
      481 cagttttaag gagcttgagg aattttcaat gtatattgaa gaaagagtca ttatcaaaga
      541 ttacgatcct gagaagctcg gatatcagat agaactgaac gaagaaggtg agttcaataa
      601 tattctttca tatccttatc ttggtagcgc atgcgatctt tcacttgaag atgcagtaag
      661 agcagcaaga aaatacgact cgctatgtgt gccggcacac atattcagat cttttggtct
      721 tataacccag ttgggtatac cgcctgaaat tgaatttgac ggggtggagg taaaaaatcc
      781 aagggaatat gagacagcca aaaaacttgg tttttcaaga ttcctgtttg gaatggatgc
      841 acatactcct gacgaactcg aaggaatagc gtgcacagta gaaactgaaa aaagagattt
      901 tgagcattta aagacagcgt tatataaagg taaggtgttt ccgttatggc aacactgaag
      961 actattgccg atcatattat ggatatttgt gagaatgctg tccgttcaaa gagcaaactt
     1021 tgtgaacttt acattgatga atatgaaaca tccttttttt tcaggatatc agacaaaggt
     1081 ataggaatgg atgagcagga agtaaaaaag gcgctagacc cgttctatac aaccaaaacc
     1141 gagcgcagga agaagttcgg tatgggtctt ccttttttga agttttccag tgaacttaca
     1201 ggcggcaatt ttaaaataac ttcagaaaaa ggctatggta ctgtcgtaga agttcttttt
     1261 aaaaaagaca gcattgactg ccagccggta ggagacattg ctcaggcgat tttttctgta
     1321 gtatatcttg accacgatac agattggtat attaagagaa ccaaaaatag tgaagcttat
     1381 gagataaact cttcagatat gaagaaagag tatggagaaa cttttttttc gccttcattt
     1441 atgaaaaaca tcaaagatct aatcatttca ttagaaaatg agttctaagg attatttaag
     1501 aacgcagagt taaaatcaga tatcatctat caataacggg aggaaagtat gacaaaaaag
     1561 atatttcttg tagcagtgct ctgcctttct gtgcttactg ctttttcaat aaccaaactt
     1621 cagaaaattg agtacagcga aaatatttct tatgcagcat cagaaattag tagaattttt
     1681 gaaaactatg agataaaaga aggcaatatg attggtgaga tagatgttaa actggcaata
     1741 aagcagataa tggaactggg gtactttaca tccatggact ttgagctaaa taacgatact
     1801 ggagtattat tattta
//
LOCUS       DAOSHR010000330         1806 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_66195_length_1806_cov_4.096516, whole genome shotgun sequence.
ACCESSION   DAOSHR010000330 DAOSHR010000000
VERSION     DAOSHR010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66195_length_1806_cov_4.096516"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1806
                     /locus_tag="PLU28_11295"
     CDS             <1..>1806
                     /locus_tag="PLU28_11295"
                     /inference="COORDINATES: protein motif:HMM:NF018899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HOG35687.1"
                     /translation="NLPLNLKEYKNSLINIQTAYQSKGYQFINLSSNILIDGESFTMQ
                     ATSVNNKMSQDGELVFVVEELPLWDIVLEGEINALDKVALKKKLGFSFRKDYEDAFFL
                     FRPQAKETYPTLEKLQALYSALVQTGYFGEETSVDFAATKNEKSGANAINIVIGGKLR
                     QIVDQDIQVKNIKFSGNQSLEFFKLHDTVKKNMTIEDSVSNTELLKSLDALRELYKSE
                     GYLFVEADVSYDQNNSELTYIITEKRYGDINITKEATSQTKDYLLNSFVKIGKGDKIT
                     QKSLQDTYYGFMGTGFFKNVDIVPVSMDETTVSFKISPYENDKPSKMIVNFNWTSPEN
                     KPFYFGFSGGAELKWANLFGAGHTVSVKGQVTPLSEYYEGSFGYNVMKLNQTKLDLGI
                     ETGALYTPKGKYEDTINATVSVQTGVKLSPSYNISDFSYITNYLSYDHYMMKDSQVTI
                     DRGSGSLGYSYIRLDSPSRPYNGEYLSIAGFSGFDLNDFSKSYYGGGIEGKLFKSLYK
                     FTFGARAKVGHVIDNGGLYSYSVGGMSTVRGYDFGERKGDSTLLFNAELAFELIKQPL
                     PIDFFIFYDIGNADNNYSKLMNNTISSAGIGAKIT"
BASE COUNT          621 a          279 c          370 g          536 t
ORIGIN      
        1 aaaatctgcc cctgaatctg aaagagtata aaaactctct tataaatatt cagactgcat
       61 accagtcaaa gggatatcag tttataaatc ttagctctaa cattttaatt gacggagaaa
      121 gctttaccat gcaagcaacc tctgtaaata ataaaatgag tcaggatggt gaacttgtat
      181 tcgttgtaga agagcttcct ctttgggata tcgtgcttga aggcgaaata aatgctttag
      241 ataaggttgc tttgaagaag aagcttggat tttctttcag gaaagattat gaagatgctt
      301 tttttctttt cagaccacag gcgaaggaaa catatcctac tcttgaaaaa cttcaagcat
      361 tgtattcggc gcttgttcag actggatatt tcggagaaga aacaagtgta gactttgcag
      421 caacaaagaa tgaaaaaagc ggagcaaatg ccataaatat agtaattgga ggaaagctta
      481 gacagatagt ggatcaggat atacaagtaa aaaatataaa attttccgga aaccagtcgc
      541 ttgaattttt taagcttcat gatacagtaa aaaagaatat gactatagaa gattctgtat
      601 caaacacaga gcttctgaaa agccttgatg cacttagaga gttgtataaa agtgaaggat
      661 atctttttgt cgaggcagat gtttcttatg atcagaataa ttcagaactt acatacataa
      721 taaccgaaaa acgttacgga gatatcaata tcacaaagga agctacaagc caaaccaagg
      781 actatctttt aaactctttt gtaaagatag gtaagggaga taagataacc cagaaatctc
      841 ttcaggatac atattatggg tttatgggaa ctggtttttt taagaatgta gatatagttc
      901 ctgtttcgat ggatgaaaca acagtttctt ttaaaatatc tccatatgaa aacgataaac
      961 cgagtaagat gatagttaat tttaactgga caagtcctga aaataagcct ttctactttg
     1021 gattcagtgg aggtgcagag ttaaaatggg ccaatctgtt tggagccgga catacggttt
     1081 cagtaaaagg acaagttaca cctctttcag agtattatga aggttctttc ggctataatg
     1141 ttatgaagct taatcagacc aagctggatc tgggtattga gacgggagct ttatacacac
     1201 ctaaaggaaa gtatgaagat actataaatg caactgtttc tgttcagaca ggtgttaaac
     1261 tgtctcctag ctacaatatt tcagactttt catatattac caattactta tcttatgacc
     1321 attatatgat gaaagattcg caggtgacca ttgacagagg ctcaggctct ttaggctaca
     1381 gctatataag actcgatagt ccaagtagac cttacaacgg agagtatctc tccatagccg
     1441 gttttagcgg atttgatctt aacgattttt ccaaaagcta ctatggaggg ggaatagaag
     1501 ggaaattatt taagagcctt tataagttta cttttggagc aagggcaaaa gtcggtcatg
     1561 taatagataa cggaggtttg tacagttatt cggtcggagg tatgagtact gtcagaggat
     1621 atgactttgg tgaaagaaaa ggtgacagta cgttactatt taacgctgaa ctggcttttg
     1681 aacttataaa acagccgctg ccgatagatt tttttatttt ctatgatata ggaaatgctg
     1741 ataacaacta ttccaaactg atgaacaata ctatttcttc agcaggaata ggagctaaaa
     1801 taacag
//
LOCUS       DAOSHR010000331         1795 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_66755_length_1795_cov_3.975287, whole genome shotgun sequence.
ACCESSION   DAOSHR010000331 DAOSHR010000000
VERSION     DAOSHR010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66755_length_1795_cov_3.975287"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            39..881
                     /locus_tag="PLU28_11300"
     CDS             39..881
                     /locus_tag="PLU28_11300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35688.1"
                     /translation="MKKKVLFFVILSINLFLWATEIKYDTAFGKKTDVPVFSYFTGAG
                     YSYPSGGEVFAGFYDYEPYYIQQNLFFKTIKIKTGERVFLSYVEIPAFGSDFSTGYEY
                     GVENTIFGLLNTFSYTRTDVGKFDYEKIGYSLVQGALPTFFNFSFQNIYYNEIANLKV
                     SIKMMRGFELPVTFQADSFTAKGILDFGLLNCFYSGVELLSFDKYYLDGFSMGACFYK
                     DKFFPAVEFAIPLNIFDQDISAGVRIGMDHSNPFSLYLINRNIKLPIILFFNKDGGGL
                     FVRL"
     gene            868..>1795
                     /gene="hemW"
                     /locus_tag="PLU28_11305"
     CDS             868..>1795
                     /gene="hemW"
                     /locus_tag="PLU28_11305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HOG35689.1"
                     /translation="MSDYKSAGLYVHLPFCKSRCSYCDYCSVTDTSVITEYFLSLYNE
                     IQMRSDKTILIDTLYIGGGTPSFIEIRFIEELVQKVKRSFITDIKEFVIEVNPDSLTE
                     EKCVLYNNLGINRVSMGIQSLDNKILGNVKRIHTAEEALESYLLASKYFENINVDFIL
                     GLPGETAESVSNNLSFIRQYKPDHISYYIYDNSHDTLLKRMIDAKLVSMPDEDLISDW
                     LDEIYLFLEQEGYERYEISCWAKNGKSCLHNKKYWQNLSYLGIGASAGGHLKNLRYVN
                     TDSVKAYIESFLNGEYCYSVYSENSQEQEFIES"
BASE COUNT          572 a          279 c          362 g          582 t
ORIGIN      
        1 gtaagagagc ttctggacag ttaattcggg aggaatctat gaaaaaaaaa gttttgtttt
       61 ttgtcatact tagcataaat cttttcctat gggcaacaga gataaagtac gacacggcct
      121 tcggtaaaaa aacagatgtt cctgtatttt catactttac aggagcaggt tacagttatc
      181 catcaggagg agaggtgttt gcaggatttt acgattatga accatactat atccagcaaa
      241 acctgttttt taaaaccata aaaataaaga caggcgaaag ggtgtttctt tcatatgttg
      301 aaatacctgc ctttggtagt gacttctcaa cgggatacga gtacggggta gaaaatacga
      361 tctttggtct tttaaatact ttttcctata caagaacgga tgtcgggaag tttgactatg
      421 aaaaaatagg atattcactc gttcagggtg cattgcctac tttttttaat ttttcctttc
      481 agaatattta ctacaatgaa attgcgaatt tgaaagtaag tataaagatg atgagaggtt
      541 ttgaacttcc ggttactttt caggcagact ctttcacggc aaaaggtata ttggatttcg
      601 gacttttgaa ctgtttttat tccggagttg aacttctgtc ttttgataag tattatcttg
      661 acggtttttc catgggggcg tgtttctaca aagataaatt ttttcccgct gtagagtttg
      721 caatcccact taatattttt gaccaggata tctctgcagg cgtaagaatc ggaatggatc
      781 acagcaatcc tttttcatta taccttatca acaggaatat aaagcttccc ataattcttt
      841 ttttcaataa agatggcggt gggctttttg tcagattata aaagtgccgg attatacgta
      901 caccttcctt tctgcaagag cagatgcagc tattgtgatt attgctcggt gacggataca
      961 tctgttataa cggagtactt tttgtctctt tataatgaga ttcaaatgag aagcgataaa
     1021 accatactta tagatacgtt gtatataggc ggagggacgc cgtcatttat agaaatccgt
     1081 tttattgaag agctggtaca aaaagtgaaa agaagcttca tcactgatat aaaagaattc
     1141 gtaatagaag ttaacccgga cagtcttaca gaagaaaagt gtgtactgta taataacctt
     1201 ggaataaaca gagtttctat gggtattcag tctcttgaca ataaaatact tggtaatgta
     1261 aaaaggatcc ataccgctga agaagcttta gaaagctatt tacttgccag taaatatttt
     1321 gagaacataa acgttgattt cattctcgga cttcctggag aaacagcaga gagtgtcagt
     1381 aataaccttt cgtttataag acagtacaag ccggaccata tttcatatta tatttatgat
     1441 aactctcatg atactctttt gaagagaatg atagacgcaa aactggtaag tatgcctgat
     1501 gaagatttga taagtgactg gcttgatgaa atctaccttt ttttagaaca ggaaggttac
     1561 gaaagatatg aaatatcttg ttgggcaaaa aacggtaaga gttgtcttca taataaaaag
     1621 tactggcaaa atctttctta tcttgggatt ggagcaagcg ccggcggtca tttgaaaaat
     1681 cttcgatacg ttaatactga ttcggtaaaa gcgtatatag aaagtttttt aaatggagaa
     1741 tactgttatt ctgtttattc tgagaactcc caagagcaag agtttattga atcca
//
LOCUS       DAOSHR010000332         1775 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_67935_length_1775_cov_3.724419, whole genome shotgun sequence.
ACCESSION   DAOSHR010000332 DAOSHR010000000
VERSION     DAOSHR010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67935_length_1775_cov_3.724419"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="PLU28_11310"
     CDS             <1..578
                     /locus_tag="PLU28_11310"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HOG35690.1"
                     /translation="PSLCVRKKDEKSFAEGIIRCSIPTHNTNLALEGAMGLGFAYHYL
                     ACGCSLEQVMEKTLSGCRIGEDNSEYKVVGAGTYERIKLIEKYIPSFKTDDELSDFLY
                     KVVGTGLGTNEVVPCAIGVFLYCGKDVFRAIKISSRLGGDTDTIGAISGALCCLYAKT
                     HNIPQEYIDKISSLNNIDFKEYAHMIAEVFI"
     gene            744..1214
                     /locus_tag="PLU28_11315"
     CDS             744..1214
                     /locus_tag="PLU28_11315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35691.1"
                     /translation="MDKDLFSGPGLVYKDIFKVNGFAFGTGYELLCLLKDALPNKELR
                     VQVSFKKLLELLKIKLNAELPFELSDIEVVIELSELYTNGLDLYNLNLELNSLIIVNI
                     GDLMKTPPAELTFKQICDFFEENFLNITTVTTDKLSLKIGLVPGIYAVLNADMK"
     gene            complement(1398..>1775)
                     /locus_tag="PLU28_11320"
     CDS             complement(1398..>1775)
                     /locus_tag="PLU28_11320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35692.1"
                     /translation="KVFISKSAGNPNSDFKVIGMPYVGEPFSACTDSLIPIGKFDTMK
                     EASHLKKYLKTKFLRFLVSILKTSQNVTQIVYGFVPHQDFTTKSDIDWSKSVSEIDSQ
                     LYAKYGLTDEEITYIEEKIKPME"
BASE COUNT          532 a          333 c          349 g          561 t
ORIGIN      
        1 ctccaagcct gtgtgtaagg aaaaaagatg aaaagtcttt tgcagaagga atcataagat
       61 gctcaatccc tacccacaat accaatcttg cactcgaagg agctatggga cttggctttg
      121 cctaccacta ccttgcctgc ggctgcagcc ttgaacaggt aatggagaaa actctttcag
      181 gatgccgtat aggtgaagac aactccgaat acaaagttgt aggagcaggc acatacgaaa
      241 gaataaagct gattgaaaag tatattccca gcttcaaaac cgatgatgaa ctttccgact
      301 tcctttacaa agtagtcgga actggtttgg gaacaaatga agtggtgcct tgcgcaatag
      361 gagtctttct ctactgtggg aaagatgttt tcagagccat aaaaatttca agtaggcttg
      421 gcggagacac cgataccatc ggagcaatct caggggcatt atgttgtctt tatgcaaaaa
      481 cccataatat accgcaggag tatatagata agatcagtag ccttaataac attgatttta
      541 aagaatatgc acatatgatc gctgaagttt tcatatagcc ctagctgccc tttggcagga
      601 acataccttt agcataccgg aagagctttc ttccggtttt tttattaact tcagctttta
      661 atctaaccaa ttcatgaaaa aatatctgtt ttatgttttt gcttttaaat acgttctttt
      721 tttccttaaa actgacccag ctgttggaca aagatctttt cagtggtcca ggactggttt
      781 ataaagatat tttcaaggta aatggctttg ccttcggaac gggttacgag cttttgtgcc
      841 tgctaaagga tgcacttcct aacaaggaac tcagagttca ggtttccttt aaaaagctcc
      901 tggaactttt gaagataaag ctgaatgctg aacttccttt tgaactttcg gatattgaag
      961 ttgttattga gctttctgaa ctttacacca atggattgga tctttacaat ctgaaccttg
     1021 aactgaacag ccttattata gtgaatatag gagatctcat gaaaactccg ccagccgaac
     1081 tgacctttaa gcagatctgc gatttttttg aagaaaactt tctgaatata acaacagtca
     1141 caacagataa gcttagtttg aaaattggtt tggttcctgg tatttatgcc gtacttaacg
     1201 cagatatgaa ataaaagatt ccccgcatga agagcgttca acttagggat ttacgaaaac
     1261 aagtaaattt ttgaccgact ctcttcaagc ggaggcgaaa aaagactgca ctgaggataa
     1321 aacctttggt gcagtcttta ttattgagaa aatgacttct tgacaggttg ttttcaaaaa
     1381 gcttggatat agtagcctta ttccataggc ttaatctttt cttcaatgta agtgatttct
     1441 tcatcggtca agccgtattt tgcgtaaagt tgactgtcga tttcagaaac gctcttgctc
     1501 cagtcgatgt ccgatttagt ggtgaagtct tggtgcggca caaatccata tacaatttga
     1561 gttacatttt gtgatgtttt caaaatagac actaaaaatc ttaaaaactt tgtttttaaa
     1621 tattttttta agtggcttgc ctctttcatt gtatcaaatt tgcctattgg gataagtgaa
     1681 tctgtgcacg cactaaaagg ttccccgaca tatggcattc ctattacctt gaaatcactg
     1741 tttggattcc ctgctgattt tgaaataaag acttt
//
LOCUS       DAOSHR010000333         1769 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_68277_length_1769_cov_6.267795, whole genome shotgun sequence.
ACCESSION   DAOSHR010000333 DAOSHR010000000
VERSION     DAOSHR010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68277_length_1769_cov_6.267795"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="PLU28_11325"
     CDS             <1..638
                     /locus_tag="PLU28_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOG35693.1"
                     /translation="GGTLFIENIQSATPFVQSKILKYLKDNEFDSQCYDYKPDVRLIF
                     SSPDDLKTLSSIGKFSSEIYYKISVVTINIPPLRERKEDIPALAKQILHALNQKYGRI
                     IYDFTEDSLSKLLNYNWLGNIRELENLIDRAILNLGPADRIITSAHIPDIVESNERST
                     GNIKELLDEYEKKIIIEMLEVCHGNKTEAAKRLGLTVRNLYYKLDRYGIKS"
     gene            635..1111
                     /gene="fliJ"
                     /locus_tag="PLU28_11330"
     CDS             635..1111
                     /gene="fliJ"
                     /locus_tag="PLU28_11330"
                     /inference="COORDINATES: protein motif:HMM:TIGR02473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HOG35694.1"
                     /translation="MRFNFSLERIMKLRKKFEDLAREELSKKIKERQEVEERIHTIDL
                     KISEFEREFNNELKFTISPQKLQQLIEYREFLKKERIRLIKFLQKKLSEENVARQEYL
                     KKKNEREVLEKLRERKYDDFMTELRKIESKEMDEVAQNKFLRNKTNSDENNNKKQA"
     gene            complement(1133..>1769)
                     /locus_tag="PLU28_11335"
     CDS             complement(1133..>1769)
                     /locus_tag="PLU28_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HOG35695.1"
                     /translation="DFKVTFMPKPMYKMPGSGMHVHQFMEKGGASIFIGDKIHGLSEL
                     ALNYLCGILDHSLSGSLLGFTNPSTNSYRRLVPGYEAPISATFAQGSRSAAIRIPGYL
                     KKNSVRIEYRTADASCNIYYALSAMVLAGIDGINKKSDPVKKGYNSDVELEDKIFPID
                     LRAVLSGLKKDSEYLRVVFPESLINEWIKIKSKEAEYIYNAPTSQEYELYF"
BASE COUNT          620 a          288 c          341 g          520 t
ORIGIN      
        1 atggaggaac tttgtttatt gagaatatac agtcagctac tccgtttgtt cagtcaaaga
       61 ttctaaaata ccttaaggat aatgagtttg atagtcaatg ttatgactat aagccggatg
      121 tgagattgat attttcaagt cctgatgatc ttaaaactct ttcaagcata ggtaagtttt
      181 cttctgaaat atattataaa atttctgtgg tgaccattaa tattccgcct ttacgagaaa
      241 gaaaagaaga tatcccggcg cttgcaaaac agatacttca cgcattgaat caaaagtacg
      301 gaaggataat ttatgatttt actgaggatt ctttaagcaa acttcttaac tacaactggc
      361 taggtaatat tagagaactg gaaaatctta ttgatcgagc cattcttaat cttggtcctg
      421 ctgacagaat aataacctct gcacatatac cagatattgt cgaaagcaat gagcgcagta
      481 ccggtaatat aaaagaactt ttggatgaat atgagaaaaa aattataata gaaatgcttg
      541 aagtttgtca cggaaacaaa acagaagcgg caaaaagact cggtctgaca gtgagaaacc
      601 tttattataa gctggacaga tatggtataa aatcatgagg tttaacttca gccttgagag
      661 aataatgaag ttaagaaaaa aatttgaaga tctagctagg gaagagcttt caaagaagat
      721 aaaagaaagg caggaagttg aagaaagaat tcatacgatt gatttaaaga taagtgaatt
      781 tgaaagggaa tttaataatg agcttaagtt tacaatttct ccccaaaaac ttcagcagct
      841 tatagagtat cgggagtttc ttaaaaaaga aagaatccgt ttgataaagt ttcttcagaa
      901 aaaactttct gaagaaaatg tggcgcgcca agagtatcta aaaaagaaga atgaaaggga
      961 agtgcttgaa aagctaagag aaagaaaata tgatgatttt atgactgagc tgagaaagat
     1021 tgaaagtaaa gagatggatg aagtagctca gaataaattt ttacgtaata aaacaaattc
     1081 tgatgaaaat aacaacaaaa aacaggctta acgcctgttt tttgttgtta ttttagaagt
     1141 aaagctcgta ttcctgtgaa gtgggagcat tataaatata ctctgcttcc ttggacttta
     1201 tttttatcca ttcattgata agagattctg ggaaaacaac cctaagatac tcgctgtctt
     1261 tcttaagacc actaagaacc gctctgagat caattggaaa aatcttatcc tcaagctcaa
     1321 catctgaatt atagcctttt ttaacaggat cacttttctt gttgatgccg tcgattcctg
     1381 caagcaccat agcggaaagt gcataataaa tattacatga agcatcagct gttctgtact
     1441 ctatccttac tgagtttttc ttaagatatc ccggtatcct tatcgcagcg cttctgcttc
     1501 cttgtgcaaa agtagcgctt ataggtgctt cataacccgg tacaagtctt ctgtacgagt
     1561 tggtgcttgg attggtaaaa cctaaaagcg agcctgacaa actgtggtca agtataccac
     1621 acagataatt aagtgcaagt tcactcaatc cgtgaatctt gtcacctatg aaaatacttg
     1681 ctccgccttt ttccataaac tggtgaacgt gcataccgct tccaggcatc ttatacatgg
     1741 gcttgggcat aaaagtgacc ttaaagtca
//
LOCUS       DAOSHR010000334         1768 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_68359_length_1768_cov_2.904845, whole genome shotgun sequence.
ACCESSION   DAOSHR010000334 DAOSHR010000000
VERSION     DAOSHR010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68359_length_1768_cov_2.904845"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            26..769
                     /locus_tag="PLU28_11340"
     CDS             26..769
                     /locus_tag="PLU28_11340"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOG35696.1"
                     /translation="MERYKYKVVVVTGGATGIGKEIAKAYGAEAASVVIADTDIDSAI
                     KTKDEILSCAGDCEFFRTDVRKEEDVISLMNFIGQKYGKIDVLINNAGVFDKINIQDM
                     TLNHWQMVIDINLTGPFLCSKYALEHIKPSGGSIVNISSTRALMSEGDSEAYDASKAG
                     LLGLTRSLAVSLSERRIRVNAVSPGWIETKGQELRIVDHLQHPAARVGIPSDVAGLCL
                     FITSSEAGFITGANFVVDGGMTVKMIYES"
     gene            complement(771..>1768)
                     /locus_tag="PLU28_11345"
     CDS             complement(771..>1768)
                     /locus_tag="PLU28_11345"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOG35697.1"
                     /translation="LVNGKTSKLTSQFPSTTTAEITTLHSGMQVQQHGIMEWFYYEPE
                     FDDIFAPFMYRLPAQKNTVLSRTSDTIDKVFDFDNIYSVFKENSIESFLFMPASIVNS
                     PYTRKAVRNSIPVSYTTFAQGLSELLQMVTEQEKGYFQFYYSELDSICHLYGPYSKEA
                     QAQLTCFMNSLEEILFKKLPRDGKTLFILSADHGQTTVKLDQGVYINSMENVVAALKR
                     NSAARPLTPGGSYRDFFIHVQHNMIDEITSILAKNLESTAKVVKTKELIERNFFGSYG
                     ASKKFLSRVGDICILPYQNKGVWWYEEDTFSMDFKGMHGGLTKEEMEIPYILYEL"
BASE COUNT          520 a          312 c          407 g          529 t
ORIGIN      
        1 tagcattgag tgaaggcgat aaaaagtgga aaggtataag tataaggttg ttgtggttac
       61 cggaggagct accggcattg gtaaggagat agcaaaagca tacggagcag aggctgcaag
      121 cgttgttata gccgatacag atatagatag cgcaataaaa accaaagatg aaatcctatc
      181 ttgtgcaggg gactgtgaat ttttcagaac agatgtccga aaagaggagg atgtaatatc
      241 gctaatgaac tttatcggtc aaaaatacgg aaaaattgat gtgcttataa acaatgcagg
      301 ggtatttgat aagattaaca ttcaggatat gactttgaat cactggcaga tggttataga
      361 tataaatctt actggacctt ttctttgcag caagtatgcc cttgaacata taaagccttc
      421 aggcggaagt atagtaaaca tctcaagcac aagagccttg atgtccgagg gtgacagcga
      481 ggcctacgat gcttccaagg caggtcttct tggtcttaca agatctttgg cggtcagtct
      541 gagcgaaagg cgtataagag taaatgcagt ttctccgggc tggatagaaa caaagggtca
      601 ggagctgaga attgtagatc atcttcagca tcctgccgca agagtcggta ttccgtctga
      661 cgtcgccggg ctgtgtctgt ttattacaag tagtgaagct ggttttataa ccggtgcaaa
      721 ctttgttgtt gatggcggga tgacggtaaa aatgatttat gagtcatgaa ttaaagttcg
      781 taaaggatgt atgggatctc catttcttct ttggtcagcc caccatgcat acctttgaag
      841 tccattgaaa aggtatcttc ctcataccac catacccctt tattctgata aggaagtata
      901 cagatatctc cgactcttga taaaaatttt ttacttgcac cgtaggaacc aaaaaaattc
      961 cgttctatca gttcctttgt tttaacgact tttgctgtag attcaagatt ttttgcaaga
     1021 attgaagtta tttcatcaat catattatgc tgaacatgga taaaaaagtc tctgtaagaa
     1081 ccacccggag tcaaaggcct tgcagcagag ttccttttaa gcgcagcgac gacattttcc
     1141 atactgttta tatatactcc ctggtcaagc tttacagttg tctgaccatg atcggcagaa
     1201 agtataaaaa gagtttttcc atctcttggg agttttttaa aaagtatttc ctccagcgag
     1261 ttcataaagc atgtcagctg tgcctgtgct tccttggagt atggtccgta caggtgacat
     1321 atggagtcca attcagaata atagaactga aaatagcctt tttcctgctc tgtcaccatt
     1381 tgaagcagtt cggataatcc ctgtgcaaaa gttgtgtagc ttacaggtat ggagtttctg
     1441 acagcttttc tggtgtatgg ggagttgaca attgaggcag gcatgaagag aaaagattca
     1501 atactgtttt ctttaaaaac agagtagata ttgtcaaaat caaaaacttt atctatagta
     1561 tcagatgttc tgctcagtac agtgtttttc tgtgccggca gtctgtacat aaatggtgca
     1621 aagatatcgt caaattcagg ttcatagtag aaccattcca ttatgccgtg ctgctgaacc
     1681 tgcattccgc tatgaagggt tgttatttca gcagttgtgg ttgaggggaa ctgtgaggta
     1741 agttttgagg ttttaccgtt tacaagca
//
LOCUS       DAOSHR010000335         1751 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_69336_length_1751_cov_4.508844, whole genome shotgun sequence.
ACCESSION   DAOSHR010000335 DAOSHR010000000
VERSION     DAOSHR010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69336_length_1751_cov_4.508844"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="PLU28_11350"
     CDS             <1..354
                     /locus_tag="PLU28_11350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35698.1"
                     /translation="LDNTVVFSHYYRYNINKDQVIRSIFEKYPEIIFFSSHSHHCFEL
                     PGNFYTDPSGFKCVDTSSVSRPTYIDTKEGVEKEWVPSSEGVYVEVFKDKVVIKAREF
                     KKSEWIKDAQFIINY"
     gene            complement(389..>1751)
                     /locus_tag="PLU28_11355"
     CDS             complement(389..>1751)
                     /locus_tag="PLU28_11355"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOG35699.1"
                     /translation="LKMNLYKGKDFGFTDLDNFVKDIFSGKSGFSFVNNQKTGKSVIM
                     YSPVPSTPNWVLGITISAKELTKDADKITYTIVLIILVILAVFVLVSFVMGNTISRPI
                     KKLSGAIDEFGKGDLTKTFVSKSKDETGKMAISLNQMAKNLRSTMTSVNTAVKDITAS
                     STDLASVSQESGAAIEELSSQVETISMNTLSAAVSVEEVTKGVEEISNQSQKIADSSK
                     ELESIARSTLESSIKGKDSIKSIAASINEVVKQSQSNETDVKSLAENAGSIEKIVNTI
                     NGITEQTNLLALNAAIEAARAGDAGKGFAVVAEEIRKLAEESKKATHEINDILTVIKE
                     KSESANKATSVTAGMIKNIDGETQNTFKQFEDIEKKVQNINTMINNLSHSIEEQSLST
                     QEITNAINNVAKTVKEISDQVKDANSAISNQSSGAQQVSSSASILAELSDKLLEQISS
                     FKV"
BASE COUNT          480 a          346 c          310 g          615 t
ORIGIN      
        1 ctggataaca cagttgtttt ttcgcattac tacagatata acataaacaa agatcaagta
       61 atacgtagca tctttgaaaa atatcccgaa ataattttct tttcttctca ctctcatcat
      121 tgttttgaac ttcccggtaa cttttatacc gatccaagcg gttttaaatg tgtagataca
      181 agttctgttt ccagaccaac atatatcgac actaaagagg gtgttgaaaa agagtgggtg
      241 ccttcaagcg aaggagttta tgtagaagtt ttcaaagata aggtagtaat aaaagcaaga
      301 gagtttaaaa aatcagaatg gattaaagat gcacagttta tcataaatta ctgaaaaaca
      361 aaacggccgg gaatccgacc gttttgtttt atactttgaa agagcttatc tgctctaaaa
      421 gtttatctga aagctctgct aaaattgaag cagaactgct tacctgctga gcgcctgagc
      481 tttgatttga gatagcgctg tttgcatcct ttacctgatc gcttatctct tttactgttt
      541 tggcaacatt gtttatggca ttggtaattt cttgtgttga aaggctttgt tcttctatcg
      601 aatgcgacag attatttatc attgtgttta tattctgaac cttcttttct atatcctcga
      661 actgcttgaa ggtgttttga gtttcaccat ctatgttctt tatcattcct gctgtaacag
      721 aggtcgcctt attagcgctt tctgattttt cctttataac tgttagaatg tcatttatct
      781 catgagtagc ttttttactt tcttctgcaa gctttcttat ctcttcggca accacagcaa
      841 aaccttttcc tgcatctcct gctctagcag cctcaatagc agcatttaga gcaagaagat
      901 ttgtctgttc cgttatgccg tttatggtat tgactatctt ttctatagat ccggcattct
      961 cagcaagtga tttaacatct gtttcattgc tctgagactg ttttacaact tcatttatcg
     1021 aagcagcaat tgattttatc gaatccttgc ctttgataga agattccagc gtacttctgg
     1081 caatactctc cagctctttc gaactgtctg ctatcttctg cgactgattg gaaatttctt
     1141 caactccttt ggttacttct tctacagaaa ccgctgcgga aagtgtgttc atacttatcg
     1201 tttccacctg tgacgaaagt tcttctattg ccgctccaga ctcttgcgat acagatgcaa
     1261 gatctgtcga agaagccgtt atatctttga ctgctgtatt aactgaggtc atagtacttc
     1321 tgagattctt agccatttga ttaagagata tggccatctt tcctgtttca tccttgctct
     1381 ttgatacaaa tgtctttgtc agatcgcctt tgccgaactc atctatggca ccggaaagtt
     1441 tttttatagg tctggaaata gtattgccca tcacaaagga aaccaagaca aagacagcaa
     1501 gtataacaag tattatcaag acaattgtat aggttatctt atcagcatct ttggtaagtt
     1561 ctttggcaga tatagttatc ccaagcaccc agtttggagt tgatggaaca ggagagtaca
     1621 ttattacaga ctttcctgtt ttttgattat ttacaaagga aaagccggac ttaccggaaa
     1681 atatgtcttt cacaaaattg tccagatctg tgaatccaaa atctttaccc ttataaaggt
     1741 tcatcttcag c
//
LOCUS       DAOSHR010000336         1747 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_69587_length_1747_cov_3.700355, whole genome shotgun sequence.
ACCESSION   DAOSHR010000336 DAOSHR010000000
VERSION     DAOSHR010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69587_length_1747_cov_3.700355"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="PLU28_11360"
     CDS             complement(<1..261)
                     /locus_tag="PLU28_11360"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-guanine transglycosylase"
                     /protein_id="HOG35700.1"
                     /translation="MNDVSPIKYELLHIDRYTNARRGRIYTKHGIIETPVFMPVGTNA
                     TVKAITGQSLEDVGSEIILGNAFHLFLRPGLDVLDSFGGLHSF"
     gene            complement(277..954)
                     /locus_tag="PLU28_11365"
     CDS             complement(277..954)
                     /locus_tag="PLU28_11365"
                     /inference="COORDINATES: protein motif:HMM:NF025115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HOG35701.1"
                     /translation="MKKLSVLVIVMLISVFTMAFTSSATCTIAKNDEIIEVNMQFPIF
                     YGMKNIPTQKYINTMFFNHASEFMQSIYDMAEQGFEDSKKYDFPFMQYAAWSQYDLHF
                     LNERFMSLTMNYYQFTGGAHGLTVKTAYNIDLSTGKNLELRSIFSDDYDYSSVILKEI
                     NLQMKKAEEGTFFSDSIERIENEQNFYLTEEGIVMFFQQYEIGPYCIGIPEFFISFDL
                     LRQGLKI"
     gene            complement(984..>1747)
                     /locus_tag="PLU28_11370"
     CDS             complement(984..>1747)
                     /locus_tag="PLU28_11370"
                     /inference="COORDINATES: protein motif:HMM:NF027524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG35702.1"
                     /translation="AQSKVNSGQALGIRFSTSPHEITPEKLDILSRYKIDLIELGVQS
                     FFDDVLLAANRPHDAEDVYTAMALLDQVKIPYGLHIMTGLKESSFHKDILSGFIACCT
                     NAVTLRVHPSVVLKGSMLEQQYRQGLYTPETLEIAVEKCAIISIYAQKMEKKIIRLGL
                     CVYGKEKENIVAGPYHDSFGSIVKSRIARFILTTVDFSQLEVPMKYKPDFIGLSRSNL
                     KYLDSRISFKERPDFLLDGKELSYNELLFRIKNIY"
BASE COUNT          517 a          345 c          332 g          553 t
ORIGIN      
        1 aaaactgtga agaccgccga aagaatcaag aacatcaaga cctggtctga ggaaaagatg
       61 gaaagcattt ccaagaatta tttcagaacc gacatcctca agactttgcc ctgtaatagc
      121 ctttactgtt gcgttggttc ctacgggcat gaagacaggg gtttcaatta tcccgtgctt
      181 ggtgtatatt ctccctctgc gggcattggt atacctgtca atgtgtaaaa gttcgtactt
      241 tataggtgaa acatcgttca taaaatcctc cgttttttaa attttaaggc cttgcctgag
      301 taaatcaaag cttataaaaa actcaggtat tcctatacag taagggccta tttcatactg
      361 ctggaagaac attactatgc cttcttcggt aagataaaag ttctgctcgt tttctatacg
      421 ctcaatgctg tctgagaaaa aagttccctc ttcagctttt ttcatctgca gattgatttc
      481 tttcagaatt acggaagagt agtcataatc atcactgaag atactgcgca gctcaagatt
      541 ttttcctgtt gaaagatcta tattataggc tgtttttacg gtgagaccat gtgctcctcc
      601 tgtgaactgg tagtagttca ttgtaaggct catgaacctt tcgttcagaa aatgaaggtc
      661 atactgtgac catgcggcat actgcatgaa aggaaaatca tatttttttg agtcctcaaa
      721 tccctgttct gccatatcgt atatagactg cataaattca gaagcgtgat tgaaaaacat
      781 agtgttgata tacttctgcg tcggaatatt tttcattccg taaaagattg gaaactgcat
      841 atttacttca atgatctcat catttttggc tatagtgcag gttgcagaac ttgtaaaagc
      901 catggtaaaa acagatataa gcataactat aaccagtact gatagttttt tcattttaac
      961 acctccggta aatttgatag ttatcagtaa atatttttta ttctgaacaa aagttcgtta
     1021 tacgaaagtt cttttccatc aagcagaaag tccggacgtt ctttgaagct tattctgcta
     1081 tccagatact ttagattaga acggctcaga cctataaagt caggcttata cttcataggt
     1141 acttcaagct ggctgaaatc aaccgttgtg agtataaaac gagctattct gcttttgacg
     1201 atgcttccaa aagaatcatg ataaggcccc gccacaatat tttccttttc cttgccataa
     1261 acacaaagcc caagccggat aatctttttt tccatttttt gagcatatat acttataatg
     1321 gcacattttt ctacagcaat ctcaagcgtt tccggtgtgt aaagtccttg tctgtactgc
     1381 tgttcaagca tactgccttt tagcactaca cttggatgga ctcttaatgt aacagcattg
     1441 gtgcaacagg ctataaagcc gctcaggata tctttgtgaa acgagctttc tttaaggccg
     1501 gtcataatat gaagaccata gggaatctta acctgatcca aaagagccat tgcagtataa
     1561 acatcttctg cgtcatgagg acggttggcg gccaagagaa catcatcaaa aaacgattga
     1621 acaccaagtt caataagatc tattttatat ctggaaagaa tatccagttt ttccggagtt
     1681 atttcgtggg gagaggttga aaaccttata cccagtgctt ggccggagtt gactttagac
     1741 tgtgccc
//
LOCUS       DAOSHR010000337         1729 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_70650_length_1729_cov_5.839904, whole genome shotgun sequence.
ACCESSION   DAOSHR010000337 DAOSHR010000000
VERSION     DAOSHR010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70650_length_1729_cov_5.839904"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            132..1433
                     /locus_tag="PLU28_11375"
     CDS             132..1433
                     /locus_tag="PLU28_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOG35703.1"
                     /translation="MKKKLLKNAYIIISADFPVQKADILISENEILQVFPNGYNEKNF
                     QEIEVWDMSGKLITPGFINSHSHVAMTLFRGVADDMNFKEWLFDNMLPREELLDDESV
                     YYASMLALMEMSAKGITFSADMYFFTNSICKAFSDIGMRGCISRGLSFDDQKGWDRRL
                     DETMDTYNRYNGYKNLLEVGFGPHAPYTVPMERLAEVASLAKKLGASVQMHIYESAWE
                     KESYTFDDLDKTGIFDTKLIAAHCVQVDMQDIKTLAAKNVTVVHNPCSNLKLGNGTAP
                     VIAMLDENVNVTVGTDGAASNNSLDILGEMKIMALSQKSKYSPSQVKINDVLRMAWEN
                     GGYAFGKKMGRIEPGYCADLAVFDISKPSFYPTDLQRLKSHIVYSTGSDSVFATMVNG
                     EWIYYNGTYPLIKESDIYEKFQKSFIRIESTFNGNSVIPGN"
     gene            1390..>1729
                     /locus_tag="PLU28_11380"
     CDS             1390..>1729
                     /locus_tag="PLU28_11380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35704.1"
                     /translation="MKVLLTVTLLFPVISIAKGFEDYIEFSPVNYTVTSREISRSGKL
                     IETDLPEKCSIVSLKASRDGFDYDLYIVKFDSLIDLTGFWYRFISDYSTNLKSATSAL
                     PLIYGKIDITY"
BASE COUNT          556 a          290 c          368 g          515 t
ORIGIN      
        1 tgtcttatga acaggcgcag acagagttag aacacatact ctctgaataa gtttaaccta
       61 tatttaatac aaacaggatg aaaaaaatcc aaaaagtgtt aaaatatata aaaaacggag
      121 gagagttgtt tatgaaaaaa aagcttttaa aaaatgctta tatcataata tcagctgatt
      181 tcccggttca aaaagctgac atacttatat ctgaaaatga aattctacag gttttcccta
      241 acgggtacaa cgaaaagaac tttcaagaga ttgaagtatg ggatatgagt ggtaaactta
      301 taacaccagg ttttataaat tctcattcac atgtggcaat gacacttttt agaggtgtcg
      361 ccgacgatat gaactttaaa gaatggcttt ttgacaatat gctcccaaga gaagagcttc
      421 ttgatgatga atctgtctat tatgccagca tgcttgcact tatggagatg tccgcaaaag
      481 gtataacttt ttctgctgat atgtacttct ttacgaacag tatatgcaaa gctttttcag
      541 atatcggtat gagaggctgt atcagcaggg gtctgtcctt tgacgatcag aaaggttggg
      601 acaggcgtct tgatgaaacg atggatacat ataataggta taacggatat aaaaaccttt
      661 tagaagttgg ttttgggccg catgctccgt atactgttcc tatggaaaga ctggctgaag
      721 ttgcatctct ggcgaagaaa ctgggagcat cagtgcagat gcatatttat gaatcagcct
      781 gggaaaaaga aagctatacg tttgatgacc ttgataaaac cggcattttt gatacaaaac
      841 ttattgcagc ccattgtgtt caggttgata tgcaggatat caaaactctt gcggccaaaa
      901 acgtaacggt tgtccataat ccctgcagca atctgaaatt aggcaacggt acggctccag
      961 tgattgccat gcttgatgag aacgttaatg taacagtagg taccgatggt gccgcaagca
     1021 ataactcgtt ggatatactt ggtgagatga agataatggc gctttcacag aaatcaaagt
     1081 actcgccttc gcaggtaaag ataaatgatg tgcttaggat ggcctgggaa aacggcgggt
     1141 atgctttcgg gaaaaagatg ggaaggatag aaccaggata ttgtgctgat ctggcagttt
     1201 ttgatatttc aaaaccatct ttttatccaa ctgatcttca gaggctgaaa tcccatattg
     1261 tatactcaac cggctcggat tcagtttttg caacaatggt aaacggagaa tggatatatt
     1321 acaacggcac ttatccgtta attaaggaga gtgatattta cgaaaagttc cagaaatcat
     1381 ttatccgtat tgaaagtact tttaacggta actctgttat tcccggtaat tagtattgca
     1441 aaaggatttg aagattatat agagttcagt cctgtaaact atactgtgac atcaagagag
     1501 atttccaggt caggcaagct tatagaaaca gatcttccgg aaaagtgcag tatagtttcg
     1561 cttaaagctt ccagggatgg ttttgactat gatctatata ttgtaaaatt tgacagtctt
     1621 atagatctta ccggtttttg gtatcgtttt atatctgatt actctactaa tcttaaatca
     1681 gcgacttctg ccctgccact gatttacggt aagatagaca taacttata
//
LOCUS       DAOSHR010000338         1727 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_70781_length_1727_cov_6.555622, whole genome shotgun sequence.
ACCESSION   DAOSHR010000338 DAOSHR010000000
VERSION     DAOSHR010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70781_length_1727_cov_6.555622"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="PLU28_11385"
     CDS             complement(<1..191)
                     /locus_tag="PLU28_11385"
                     /inference="COORDINATES: protein motif:HMM:NF014586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ExsB family protein"
                     /protein_id="HOG35705.1"
                     /translation="MDGILELVEKIKEDIVQTVGQERLYVSFSGGLDSTVVALLAKDA
                     LGSDRVTLLNVCFGPYSFS"
     gene            418..>1727
                     /locus_tag="PLU28_11390"
     CDS             418..>1727
                     /locus_tag="PLU28_11390"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HOG35706.1"
                     /translation="MKFSAKLILIVLVFAILPMLVFWKVSSGETQKALKTLTLSKVQL
                     LSDKLYDDLQAYYAGFQKSAETISKIDFLPMLAKNYSDLFSDNPKMIEYVKKVYLELN
                     PENEKKVLERPSVDTQQLLEEQYGDDYYSMSDYDLFHSNYHKVLLNFTATEKLEDLYL
                     IDKNGNIIYSVCKDRDFAQNISSSDNPIGKLLTQLKDQNAENTFPVLGNFSYYESKNK
                     YMAFYGIPVKKYTIEGYLVISKEIKDIEDIVKRETDKNNTLRFFISDETNTVLNTLKT
                     SGEINFGDLRKNYLQNDGTAEYKSYDGKDVLTAYKNLVLSSGSLMLAVEIPKEIAFES
                     LRRIDLINTVIIIVVGVVVVLLITLFSSYLIKPVEKIKSSVENIARGDISKLVMIKRK
                     DEFGMMGKLFNQISGSIKDIILKIRTETAELDSSSVILKDKSQA"
BASE COUNT          594 a          308 c          293 g          532 t
ORIGIN      
        1 tttgaaaaag agtacgggcc gaagcataca ttaagcaagg ttaccctatc acttccaagg
       61 gcatctttgg ctaaaagtgc aaccaccgta ctgtcaagcc caccggaaaa ggaaacataa
      121 agtctttcct gtccgacagt ctgcactata tcttctttga ttttttccac aagctccaag
      181 atcccatcca atttgaacac ctcattcgtc tttttgagca taaaaattat acatcatatt
      241 ttttatctta agatattttt acagtcaatt aatattctcc atactcttta tatttagtga
      301 cttatgatat tgtttacctt tgttcttcta aaattttcag taatttaata tttgcaggca
      361 tctatggtat aattttatat aagaaaaact tttattattt taataagggg gaataccatg
      421 aagttttcgg caaaactcat tttaattgtt cttgtgttcg ccatacttcc tatgcttgta
      481 ttttggaagg tatcttccgg cgaaactcaa aaagctttga aaacgcttac tttatctaaa
      541 gttcagcttt tatcagataa gctttacgac gatctgcaag cttattacgc cgggtttcag
      601 aaatcggcag aaaccatatc taaaatagac tttcttccta tgcttgcaaa aaactactct
      661 gacttatttt cagataatcc caagatgata gaatacgtaa agaaggtata tcttgagctg
      721 aatccggaaa atgaaaaaaa agttttggaa cgtccatccg tagacacaca acagcttctg
      781 gaggaacagt acggtgatga ttactattcg atgagtgatt atgatctctt tcacagcaac
      841 tatcataagg tgcttttgaa cttcactgcc actgaaaagc ttgaagatct ttaccttatc
      901 gacaaaaacg gaaatatcat atattctgta tgcaaggata gagattttgc ccagaatatc
      961 tcatcttcag acaatcctat tggaaagttg ttaactcagc tcaaagatca gaacgcagaa
     1021 aacacttttc ccgtactagg taacttttct tattatgagt ctaaaaataa gtatatggcg
     1081 ttttatggaa tacctgtaaa aaagtacact atagaaggat atcttgttat aagcaaggaa
     1141 ataaaagata ttgaagatat cgtaaaaaga gaaaccgata aaaataacac tctgagattt
     1201 tttatatcag atgaaaccaa taccgtactg aatactctca aaacttccgg agagataaat
     1261 tttggagatc tgagaaaaaa ctaccttcaa aatgacggaa ccgcagaata taaatcttac
     1321 gacggtaaag atgttctgac tgcgtataaa aatcttgtac taagctccgg ttcacttatg
     1381 ctggctgtgg aaattccaaa ggaaatcgca tttgaaagcc ttaggagaat tgatcttata
     1441 aataccgtaa taattatagt ggttggtgtt gttgttgtat tactcattac cttgttctcc
     1501 agctatctca taaaacctgt ggaaaaaata aaatcctctg tcgaaaacat tgcacgcggc
     1561 gatatatcaa agcttgtaat gataaagaga aaagatgagt ttggaatgat gggaaagctt
     1621 tttaaccaga taagcggatc cataaaagac ataattctga agatacgaac agaaacagct
     1681 gagcttgaca gctcttcggt catcttaaaa gataaatcgc aggcaaa
//
LOCUS       DAOSHR010000339         1726 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_70857_length_1726_cov_4.046080, whole genome shotgun sequence.
ACCESSION   DAOSHR010000339 DAOSHR010000000
VERSION     DAOSHR010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70857_length_1726_cov_4.046080"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1370)
                     /gene="crtI"
                     /locus_tag="PLU28_11395"
     CDS             complement(<1..1370)
                     /gene="crtI"
                     /locus_tag="PLU28_11395"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HOG35707.1"
                     /translation="MLKKVAVVGAGVAGLSCAIRLQKDGYDVEIFEKDSFPGGKMNTI
                     SSAGFTFDVGPTIVMMPEVYREVFEYAGKNPEDYIPMQQLDPIYSIQFSDGQKYDVST
                     DMAHLTAFLENISMKETLGYYKYLSKTYSRYLNAKENFIERSFRGPLDFYNPKTIVNA
                     LKLRTFNTAHNEVAKYVKDERLQKLLSFQTLYIGISPYNGPSIYTIIPMIESIYGVWF
                     IKGGMFTMAKAMASLFEELGGKINYNCAVEEVLVQNGKACGVSSDRQKIKSDYVVCNA
                     DFPYAMKNLIRQSTYKGKYTDKKIDSMKYSCSVVLLYLGLKKKIDGLKVHNIIFADDF
                     DRNISDIFQGNDPKDPSIYIYASTKADSSLAPKDKEALYVLVPVPDLSVRKKRWTAEE
                     TEQYKKIIFSKMKKVNGLNDIENLVEYEKIYTLNDFESRFNAYNGATFGLAPTLGQSN
                     YFRPHN"
     gene            complement(1511..>1726)
                     /locus_tag="PLU28_11400"
     CDS             complement(1511..>1726)
                     /locus_tag="PLU28_11400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35708.1"
                     /translation="QVCAFQFWSKFRQDREKAWQDYVRLCRAGGSKPFTGLLEIANLK
                     SPFENGCLEPVSKVIESYLDSIDDKKL"
BASE COUNT          492 a          346 c          318 g          570 t
ORIGIN      
        1 ttgttgtgag gtctgaaata attgctttgt ccaagagtgg gagccagtcc gaatgtggcg
       61 ccgttataag cattaaatct tgattcaaaa tcgttcagag tatatatttt ttcatattcg
      121 accagatttt ctatgtcatt taaaccgttg acttttttca tcttggaaaa tataattttt
      181 ttatactgtt cagtttcttc ggctgtccac ctcttttttc ttacagatag gtcaggaacc
      241 ggaaccaaaa cataaagagc ttctttgtct ttgggggcaa gcgagctgtc agcctttgtc
      301 gatgcgtata tatagattga aggatctttt gggtcatttc cctggaagat atcggatata
      361 tttctgtcaa agtcatcagc aaatattata ttatgaacct tcagaccatc aattttcttt
      421 tttaagccaa gatacagtaa cacaacagaa caggagtact tcatggagtc tattttttta
      481 tccgtatact ttcccttata ggtactttgt cttatgagat ttttcattgc ataagggaag
      541 tctgcgttgc agaccacata atcgcttttt attttctgcc ggtctgaaga aactccgcag
      601 gcttttccgt tttgaacaag tacttcttca acggcgcagt tgtagtttat ctttccgccc
      661 agctcttcaa aaagtgatgc catagctttg gccatggtaa acattccgcc ttttatgaac
      721 cataccccgt aaatactttc gatcataggt attatagtat atatggaagg tccgttgtag
      781 ggcgatattc ctatgtacag ggtctgaaag ctgagaagct tttgaagacg ttcatcctta
      841 acatatttgg caacttcatt atgggctgtg ttaaaagttc tgagtttcaa agcgttgaca
      901 atagtttttg gattataaaa atccaatggt ccacggaagg atctttctat aaaattctcc
      961 tttgcattaa gatatctgga gtatgttttg gaaaggtact tgtagtaacc taaagtttcc
     1021 ttcatggaga tattttcaag aaatgcggtc aaatgagcca tatcagttga aacatcatac
     1081 ttctggccgt cggaaaactg tattgaatat ataggatcaa gctgctgcat aggtatgtag
     1141 tcttcgggat ttttcccggc atattcaaaa acttctctgt aaacctcggg catcataact
     1201 atggttggtc ctacatcaaa agtaaacccg gcggaactta tcgtattcat ctttcccccc
     1261 gggaaactgt ctttttcaaa tatttcaaca tcataaccgt ccttctgaag tcttattgca
     1321 caagaaagac cagccacgcc ggcaccgaca accgcaactt tcttcaacat gatccccccc
     1381 ttattctggt ttctatataa tattatgtag caaaaattaa aaaacggaat cgctttttta
     1441 aaatttcctt taaataaagt gaataataaa acaaaatctc ccatacggga gattttggaa
     1501 aagaaaatta ttataatttt ttatcgtcga tactgtcaag ataactttca attaccttgg
     1561 aaaccggctc aagacaaccg ttctcgaaag gggatttaag gttggcaatt tcaagcagtc
     1621 cggtgaaggg ttttgaacca ccggctctgc agagccttac ataatcctgc catgcctttt
     1681 ccctgtcctg tctgaacttg ctccagaact gaaaagcaca tacctg
//
LOCUS       DAOSHR010000340         1691 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_73048_length_1691_cov_6.670538, whole genome shotgun sequence.
ACCESSION   DAOSHR010000340 DAOSHR010000000
VERSION     DAOSHR010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73048_length_1691_cov_6.670538"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..1189)
                     /locus_tag="PLU28_11405"
     CDS             complement(110..1189)
                     /locus_tag="PLU28_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOG35709.1"
                     /translation="MLNFAIIGCGRIGTKKHIQACIENADIINLVAVCDIKKEKAEQA
                     SQIYSAAVTKKPSVYTDYIKMLESENIQAVAVATESGLHYQVATDCMYMDKNVLVEKP
                     MALSTEHMDKMIKLAKDRGLKLGVCFQNRFNAPIQELRKKVESGGFGRIFHAQASVRW
                     NRNRDYYKQASWRGTWEMDGGTLMNQCTHNTDLLIWMLGGEVEEVNGVIRNFNHDYIS
                     TEDFGGAVIKFKNGSVGILEGTSNVYEKNLDEKLSIFGENGTVVIGGLAVNKIETWRF
                     ENENSHPFQNLPDPDTVYGNGHVPLYRDFCEAVTTGREPFINGEEGKKAVQTILAIYK
                     SSLTGSPVKFPFDFSTLEMKGYFNK"
     gene            complement(1204..>1691)
                     /locus_tag="PLU28_11410"
     CDS             complement(1204..>1691)
                     /locus_tag="PLU28_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP binding domain-containing protein"
                     /protein_id="HOG35710.1"
                     /translation="LDPFYLQYKARAYDFHTTMIDLAGDITDSMPEYVVERLSEILND
                     NKKCLNGSRILLLGVAYKNDIDDMRESPALKVIEHLEKRHAQVIIHDPFVPEFSHNNK
                     KYYSSPLTKDLIENMDAVVITTGHTTVDYGFVADNAKILFDTKNVTKNMRTDRKNLFI
                     L"
BASE COUNT          424 a          360 c          305 g          602 t
ORIGIN      
        1 aagtacaaag acacaaggca catccctccc ggctgcgccg tactcccctg aagggaagat
       61 taaaatgcac tttcaaggtt aataaaaaat tcccgcttag gcgggaattt tatttattga
      121 aatatccttt catttcaagg gttgaaaagt caaagggaaa tttaaccgga ctgcctgtca
      181 aagaagattt gtagattgca agtatagttt gaacagcttt ttttccttct tcaccgttta
      241 taaaaggttc tcttcctgtt gtaactgctt cgcaaaaatc tctgtaaaga ggaacatgtc
      301 cgtttccgta aacggtatcc ggatctggaa ggttctgaaa cggatgggaa ttttcgtttt
      361 caaacctcca tgtttcgatt ttgttcacgg ccaatccacc tatcacaact gttccgtttt
      421 ctccgaatat tgaaagtttc tcgtcaagat ttttttcata gacattagat gttccttcga
      481 gaattcctac cgaaccattt ttgaatttta ttactgctcc gccaaaatct tctgtactta
      541 tgtagtcatg gttaaagttt cgtataacac cgttcacttc ttcaacttcg ccaccaagca
      601 tccatatgag cagatctgtg ttgtgcgtgc attggttcat cagagttcct ccgtccattt
      661 cccatgtgcc tctccagctt gcctgtttgt aatagtcacg gtttctattc catcttactg
      721 atgcctgtgc atggaatatt ctgccaaaac ctcctgattc aacctttttc ctaagttcct
      781 gtatcggagc gttaaagcgg ttctgaaaac atactccaag cttgagtcct ctatctttgg
      841 ccagttttat catcttgtcc atatgttcgg tagaaagtgc catgggtttt tccaccagca
      901 cattcttgtc catgtacata cagtctgtgg caacctgata gtgtagtccg ctttctgtgg
      961 ctactgctac tgcctgaatg ttttcgcttt caagcatctt tatgtaatcg gtatatactg
     1021 acggtttttt agtgactgct gcactgtaga tttgacttgc ctgttcggct ttttcttttt
     1081 ttatatcgca gacagccaca agattaataa tatctgcatt ttcaatgcac gcttgtatat
     1141 gtttttttgt tccgattctt ccgcagccga taattgcaaa attcagcatt attacctccg
     1201 tttttacaga ataaacagat tttttctgtc tgttctcata tttttggtta cattttttgt
     1261 atcgaaaagg atcttggcat tatctgccac aaacccgtaa tctacagtag tatgtcctgt
     1321 tgttattaca actgcatcca tattttctat aaggtcttta gttaatggtg atgagtaata
     1381 ttttttattg ttatgactga actcaggcac aaaaggatca tgtatgatta cttgggcatg
     1441 ccttttttct aggtgttcaa taactttaag cgccggtgat tctctcatat cgtctatatc
     1501 gtttttgtat gcaactccaa gtaagaggat ccgtgagccg ttaagacact ttttattgtc
     1561 gttgagtatc tccgaaagtc tttctacaac atactcaggc attgagtctg taatatctcc
     1621 tgccaaatct atcatagttg tatgaaaatc atatgcccta gctttgtact gaaggtaaaa
     1681 cgggtcaagc g
//
LOCUS       DAOSHR010000341         1689 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_73175_length_1689_cov_3.684823, whole genome shotgun sequence.
ACCESSION   DAOSHR010000341 DAOSHR010000000
VERSION     DAOSHR010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73175_length_1689_cov_3.684823"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..728)
                     /locus_tag="PLU28_11415"
     CDS             complement(<1..728)
                     /locus_tag="PLU28_11415"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HOG35711.1"
                     /translation="MKPKVYITRNIPEKGVENLKSLYDVKVWPDELAVPRDILKEELR
                     DCQGVITMLSDKIDSQVIDSAPNLKVISNYAVGYDNIDIKYAASKGIKIGNTPGVLTE
                     TTADLAFTLLMAVSRRITEAAEYVKQGNWKTWGPQLMLGYDIWGSTIGIIGFGRIGQA
                     MARRALGFGMKVLYYSRTRKQQAEKEMNVTYCSLEQIYQQSDFISLHTDLNPSTKGLI
                     GKEAFQAMKPSCILINTARGQVVNT"
     gene            complement(744..>1689)
                     /locus_tag="PLU28_11420"
     CDS             complement(744..>1689)
                     /locus_tag="PLU28_11420"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HOG35712.1"
                     /translation="RMVVLSIGLNRSVKLIINRLFPVEPIEGVQLVYFDDSEPAVEVL
                     CKNLDNTRTGIDKNWPSRFLIELMNKKPEMSFFNSTFMLDSLRIVKDKQEIELMKTAS
                     IINDSVMEKVIELVPELLSEHHLAKAVKNLFETQKSRFSFEPIVAYGKNGACPHHEPD
                     NSILKINEGIILDIGGMYKKYCSDMTRTVFYGKAPAEFKKVYDIVLTANLKAISFIKP
                     GVKFCEIDFQAREHISEKGFGQYFTHRTGHNIGLDVHEYADVGPSNELTVQPGMIFSI
                     EPGIYLPDKLGVRIEDLVLVTENGAEVLNRYDKNLIEL"
BASE COUNT          419 a          370 c          305 g          595 t
ORIGIN      
        1 gtgtttacca cctgtcccct ggctgtattt attagtatac agcttggttt catggcttga
       61 aatgcttctt ttcctatcaa acctttagtt gatggattaa gatcggtatg cagcgatatg
      121 aaatcacttt gttgataaat ctgttctaga ctgcagtaag taacattcat ctctttctct
      181 gcttgctgtt ttctggttct gctgtagtac agaactttca ttccaaaacc tagagctctt
      241 ctagccattg cctgtccgat tcttccgaat cctattatac cgattgtgct tccccatata
      301 tcgtatccga gcatcagttg aggtccccat gttttccagt ttccttgctt tacatactct
      361 gctgcctctg ttatcctgcg ggatacagcc atcagcagtg taaaagcaag atcagctgtg
      421 gtttcagtca gaactccagg tgtgttacca attttaatgc ccttagatgc ggcgtacttt
      481 atgtctatgt tatcgtatcc gacagcataa ttgcttataa ccttaaggtt tggagcgcta
      541 tcaattactt ggctgtctat cttgtccgag agcattgtta taacgccttg gcagtcacgt
      601 agttcttctt ttaaaatatc cctgggaacc gcaagctcat ccggccatac tttcacatca
      661 taaaggcttt tcaggttctc aactcctttt tcggggatat ttctggtgat atatactttg
      721 ggtttcactg tttttcactt cctttataat tcaataagat ttttatcgta tctattaagt
      781 acttcagcgc cgttttccgt tacaagcaca agatcctcta ttcttacgcc cagtttgtcc
      841 ggaagatata tacctggttc tatagaaaat atcattccgg gctgtactgt aagttcatta
      901 gaaggtccaa catctgcata ctcgtgcaca tccagaccaa tattgtgacc tgttctgtgt
      961 gtaaaatact gtccaaagcc tttttcgctt atatgttctc ttgcctgaaa gtcaatctca
     1021 cagaacttca caccgggttt tatgaaactt atggccttta aattggcagt taaaactatg
     1081 tcgtatactt tcttgaactc agccggagcc ttaccgtaaa aaacagttct ggtcatatcg
     1141 gaacagtatt ttttatacat tccgcctatg tcaagtatta taccctcgtt tattttcaga
     1201 atactgttat ctggttcatg atgtggacag gcgccgttct ttccgtacgc tactatgggc
     1261 tcaaaggaaa atctggattt ttgagtttca aagagatttt tgacagcttt ggcaagatgg
     1321 tgttcactaa gcagctcagg tacaagctca ataacctttt ccattacaga atcattaata
     1381 atgctggctg ttttcatcag ctctatctct tgtttatctt ttactatcct taggctgtca
     1441 agcatgaacg tagagttgaa aaagctcatt tccggttttt tgttcataag ttcgataaga
     1501 aaccttgaag gccagttttt gtctatcccg gttcttgtgt tatcaaggtt tttgcataaa
     1561 acttcaactg caggttcaga atcatcaaag taaaccagct ggactccttc tataggctca
     1621 actgggaata gcctgttgat aataagtttt acactgcggt tcaaacctat gctcagaact
     1681 accattctt
//
LOCUS       DAOSHR010000342         1686 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_73371_length_1686_cov_3.689761, whole genome shotgun sequence.
ACCESSION   DAOSHR010000342 DAOSHR010000000
VERSION     DAOSHR010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73371_length_1686_cov_3.689761"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1414)
                     /locus_tag="PLU28_11425"
     CDS             complement(<1..1414)
                     /locus_tag="PLU28_11425"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HOG35713.1"
                     /translation="MQSCFLGVDIGTTAMKILVVGQDGNTIGLYREDTPKKIIEGITF
                     LDLRRIYEYFLRIYSKIKNGYKILGVSCASISESVIPIRNAKALTDPLIWYEECTLSY
                     KASFGEKAEALSGYNVSGFENEHIFSLYKILWTRDRIKPQTVDFWIPLSSYIPFMMTG
                     KAVWDITQGCRSYMMNVHKRAWNKELADFFGLYGNLGVLKYTSSFIGYAEDGVPFFSS
                     GHDHMTGLFGICRIIGTDRIIYDSMGTASSIAGLFKKNETDQDLEHPFMGKSGNIGAA
                     FEDNQYYAENSIRFYGKFLEYLMKLVCMDIKPEDFKKINKEIESLSEVRESCIFLTGG
                     DPVVGKKNNFFNIMDACDNLTAPLLIQSGYIYLSLISKKIAEAVNSFLPRDSYYYASG
                     GNTDNKVLMQYKASLLNKDIITTDIREGTALGAVVSAITGSGCEKTLRCISDKVKKEV
                     IKPDPKVAVQIKKAEERILRR"
     gene            complement(1416..>1686)
                     /locus_tag="PLU28_11430"
     CDS             complement(1416..>1686)
                     /locus_tag="PLU28_11430"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOG35714.1"
                     /translation="ITFGKEGCIAYADKIFKAIPKKTYSDFAVGSGDSFFAGYLFESK
                     RTTDIKTRLITATACAVANTLRYGAGFFNLDEYEQQKQNVEVTEV"
BASE COUNT          445 a          372 c          295 g          574 t
ORIGIN      
        1 aacggcggag gattctttct tccgcttttt ttatctgtac tgctacttta ggatctggtt
       61 ttataacttc cttttttact ttatctgata tacaccgtaa tgttttttcg cagcctgatc
      121 ctgtaatggc acttacaacc gccccaagtg ctgtgccttc tctgatgtct gttgttatga
      181 tatctttgtt cagaagtgat gctttatact gcataagaac tttgttgtcg gtatttccgc
      241 ctgaagcata gtagtaagaa tctcttggca aaaagctgtt tacagcttca gctatttttt
      301 tgcttataag gcttaggtat atgtagccgc tttgtattaa gagaggcgct gttaagttat
      361 cacatgcatc cattatatta aaaaagttgt ttttctttcc tacaacagga tctcctccgg
      421 tcagaaagat acagctttct ctcacttcag acagactctc tatctctttg tttatcttct
      481 taaagtcctc aggcttgata tccatgcaaa ccagtttcat gagatattca aggaactttc
      541 cgtaaaaacg aatggagttt tctgcatagt actggttgtc ttcaaatgct gctcctatat
      601 ttccgctttt tcccataaaa gggtgttcca gatcttgatc agtttcgttc ttcttaaaaa
      661 gacccgctat tgagctggct gttcccatcg agtcgtagat tattctatct gtaccgataa
      721 ttcggcatat tccgaaaaga cctgtcatat ggtcgtgtcc tgaagaaaaa aacggtactc
      781 cgtcttctgc ataacctata aaagatgatg tgtacttaag cacaccaaga ttaccgtaaa
      841 gaccgaaaaa atcagcaagc tccttgttcc atgctctttt atgaacattc atcatgtatg
      901 acctgcagcc ttgggttata tcccataccg cttttccggt catcataaaa ggaatgtatg
      961 aactgagcgg aatccaaaag tcaaccgtct gcggtttaat tctatcccta gtccaaagta
     1021 tcttatataa tgaaaatatg tgttcgtttt caaaccctga tacattatat ccggacagtg
     1081 cttctgcttt ttctccgaag gaggctttgt acgaaagtgt acactcctca taccatatga
     1141 gagggtctgt caaagctttt gcattcctta tcggaataac cgattcgctt atgcttgcgc
     1201 agcttactcc cagaatttta tatccgttct ttatctttga atatatccgc agaaagtact
     1261 cgtagattct tctcaaatca agaaaagtga taccttctat aatctttttg ggggtatcct
     1321 ctctgtacaa tccaatagta ttaccgtctt gtccgactac aagaattttc attgccgtcg
     1381 taccgatatc cactcccaga aaacaggact gcatcttaaa cctccgttac ctcaacattc
     1441 tgtttctgct gctcatactc atcaagatta aaaaaacccg caccgtaacg cagtgtattt
     1501 gccacagcgc atgctgtagc tgttataagt ctggttttta tgtctgttgt tcttttgctt
     1561 tcaaataagt acccggcaaa aaaagagtcg cccgaaccga ctgcaaagtc agagtaagtt
     1621 tttttgggaa tagctttgaa aatcttatct gcgtaagcta tgcagccttc ttttccaaaa
     1681 gttatt
//
LOCUS       DAOSHR010000343         1667 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_74707_length_1667_cov_4.434864, whole genome shotgun sequence.
ACCESSION   DAOSHR010000343 DAOSHR010000000
VERSION     DAOSHR010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74707_length_1667_cov_4.434864"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            315..551
                     /locus_tag="PLU28_11435"
     CDS             315..551
                     /locus_tag="PLU28_11435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35715.1"
                     /translation="MKSLNFHGLKKPKKFEKHYEISSNKDFEDIFEEEPELASRVRTL
                     YQNIAGNKIKKMEIDFKRNGSSAELIIKLPVSIA"
     gene            658..>1667
                     /locus_tag="PLU28_11440"
     CDS             658..>1667
                     /locus_tag="PLU28_11440"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOG35716.1"
                     /translation="MKKVMFWGFLLLIMGRCFSYEITFIYTLPASSVQVAIEGLPGGT
                     YDMQRIGESFWKHDIDLQQGKYYYRYIVDGKETYDPGNNDYEIVHDKLLNYKYVGEDE
                     TGMPKIKYEHSMQRMFINPIRYGEIYLCIGVEKANNQKISIIVNGDILSSESFIVDDT
                     QYQRFHVKSNAKILKYLFICEKDKTVYNIGSSKDSSKMFEFDFDNPKISYFDPPQWAK
                     GSIYYQIFPDRFFNGDTENDPSTVRPWYEEYTSQSLSSHFYGGDLAGIIQKIDYLKEL
                     SVGCIYMNPIFEAPSTHKYNTTDYMKVDPSFGNEKTLIDLLQKAHSQEIKIVLDGVFN
                     HTG"
BASE COUNT          589 a          274 c          306 g          498 t
ORIGIN      
        1 ttaaaagcaa gtccaaactt tacggttggc gatgaagtca ttaaaagttc aaaaatatct
       61 tcagcagtct ttataaaatg tgactgccct ataatcacat tactttgagg aagaatactt
      121 acatcaaccg tttcaaagga taaactcata tttttacctc ccatattatt gaatatcgta
      181 tagactatat ctagattata cctttttttt gtgtattgaa atttaaaatt ttagcaaaca
      241 acagttaata tttatggtat aaactatata ttgaaaataa ggtatacaaa agtataatat
      301 ataaggaggt ttttatgaaa agtctaaatt ttcatggatt aaaaaaacca aaaaaatttg
      361 aaaaacatta cgaaatatct tccaataagg attttgaaga catctttgaa gaagaaccgg
      421 agcttgcctc aagagtacga acactttatc aaaatatcgc cggtaataaa attaaaaaaa
      481 tggaaataga cttcaaacgt aacggaagca gtgccgagtt gataatcaaa ctccctgtat
      541 caatagctta gtccgctgga acggtcctta taaggaccgt ttttcttatc taaactataa
      601 ccatacatct ggctgtgata taatttttat tactgcgtgc cataaggagg tagtaaaatg
      661 aaaaaagtta tgttttgggg ttttcttttg cttattatgg gaagatgctt ttcttatgaa
      721 ataactttta tctatacctt gccggcaagc tctgttcagg ttgctataga aggcctgccc
      781 ggcggaacct acgatatgca aaggatcggt gagtccttct ggaagcacga tatcgatctg
      841 cagcagggca aatactatta tcgatacatc gtcgacggaa aagagaccta tgatccaggc
      901 aacaatgatt atgagattgt gcatgataaa cttttgaact ataagtacgt aggagaagat
      961 gaaaccggta tgccaaaaat aaagtatgag cactctatgc aaagaatgtt tatcaatcct
     1021 ataaggtatg gagagatata tctgtgcata ggagttgaaa aggcaaacaa ccagaaaata
     1081 agtattatag taaacggcga tatcctttca agtgaaagct ttattgttga tgatactcaa
     1141 taccaaagat ttcacgtgaa aagcaacgca aaaatattga agtatctttt tatctgcgaa
     1201 aaggataaaa cagtatataa tattggatct tcaaaagact cttccaagat gtttgagttc
     1261 gattttgaca atccaaagat aagttatttt gatcctccgc aatgggcaaa gggaagtata
     1321 tactatcaga tcttcccgga cagatttttt aatggagata ccgaaaatga cccttcgaca
     1381 gtacggccat ggtatgaaga gtacacctca cagagtcttt cttcgcactt ttatggtgga
     1441 gatctggcag gcataattca aaaaattgat tacctgaaag agctttctgt aggctgcata
     1501 tatatgaatc ctatatttga agccccctcg actcataagt ataatacaac ggattatatg
     1561 aaagtagatc cttcctttgg caatgagaaa accttgatcg atctgctcca aaaggctcat
     1621 tcacaggaga taaaaatagt acttgatggg gttttcaacc atactgg
//
LOCUS       DAOSHR010000344         1656 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_75496_length_1656_cov_4.023111, whole genome shotgun sequence.
ACCESSION   DAOSHR010000344 DAOSHR010000000
VERSION     DAOSHR010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75496_length_1656_cov_4.023111"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..857
                     /locus_tag="PLU28_11445"
     CDS             <1..857
                     /locus_tag="PLU28_11445"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOG35717.1"
                     /translation="LGFSMVKGEKRVSESFERTDTVFEKLTGHEKELKEYLEKCLFYL
                     ENYVQLEPGAYTAILSPQAAGVFAHESFGHKSESDFMLGDENMKKEWAIGKKVGSDIL
                     SIVDSGKIPGSGYVQFDDEGTKAGETYLIKNGTLAGRLHSAYTAAALEEKLTGNARAK
                     DFEYEPIVRMTSTYILPGEKTKEQLISEVSDGILIETINHGSGMSDFTIAPRRAYRIH
                     NGKIAEPVSISVITGNVFSTLNEIDGLSDKLEILSFIMGGCGKMEQYPLSVGFGGPYV
                     RVRTINAR"
     gene            869..>1656
                     /locus_tag="PLU28_11450"
     CDS             869..>1656
                     /locus_tag="PLU28_11450"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HOG35718.1"
                     /translation="MLKEVFNSKINQVSFSVSQSKVDSIRKKDIEKTGVRVYSDGFIG
                     VAGQIGKYDSSKLTSKAVENLKNQIPYEFGKDSKVQKKISVTGNIPDEKELLMQIEEY
                     LDYCRQKYENLIFFNKINVTERQISLNNENGLDLYFSDRFINFDIVFKDKATANIMDG
                     GVIYQGRDFSLEQLKSYTEKICGAYSRSGEVKYGKKLPVIFSSYDMLVMMKFITDLNG
                     RMYGSGGSIFSNKIGEKLFSEEFTLFQSNDPFNSIPDFAFFDAEG"
BASE COUNT          559 a          278 c          371 g          448 t
ORIGIN      
        1 gtcttggatt tagcatggta aaaggagaaa agcgtgtttc ggagagtttt gaaagaacgg
       61 atacggtttt tgaaaagctt acaggtcatg aaaaagaact caaagagtat cttgaaaaat
      121 gtcttttcta ccttgaaaac tatgtccagc tggaacctgg tgcctacact gcaatacttt
      181 ctccacaggc tgcaggagtt tttgctcatg aaagctttgg gcacaagagc gaatcggatt
      241 ttatgcttgg tgacgaaaac atgaaaaaag agtgggctat aggaaaaaag gttggaagcg
      301 atatcctttc gatagttgac agcggaaaaa tacccggttc cgggtatgtg cagtttgatg
      361 atgaaggaac caaagcagga gaaacatatc ttattaagaa cgggacgctt gccggccgtc
      421 ttcacagtgc ttatacggcg gcggctttgg aggagaaact taccggaaat gccagagcca
      481 aagattttga atacgaacct atcgtcagaa tgaccagcac atatattctt cccggagaaa
      541 agactaaaga acagcttatc tccgaagttt cggacggtat tctcatagaa accataaatc
      601 acggttccgg gatgtccgat tttacaattg cacccagacg tgcttataga atacataatg
      661 gcaagatagc tgaacctgta agtatctcag taattacagg caatgttttt tcgactctaa
      721 atgagatcga tggcctttcg gataaactgg agattctttc tttcattatg ggtggctgcg
      781 gaaaaatgga acagtatccg ctttccgtag gattcggcgg accttacgta agagttcgca
      841 ccataaatgc cagataaggg ggataaaaat gcttaaagaa gtttttaatt caaaaatcaa
      901 tcaggtcagt ttcagcgtaa gtcaaagcaa agtagattca ataaggaaaa aagatattga
      961 gaaaaccgga gtaagagtat attccgatgg ttttatagga gttgcagggc agatcggaaa
     1021 atatgacagc agtaaactta catcaaaggc cgttgagaat cttaaaaatc aaatacctta
     1081 tgagtttgga aaagactcaa aggtgcagaa aaaaatctca gttacaggca atattccaga
     1141 cgaaaaagag cttcttatgc agatagaaga gtaccttgat tactgcaggc aaaagtatga
     1201 gaatttgata ttctttaaca agataaacgt taccgaaaga cagatctcgc ttaataatga
     1261 aaatggactt gatctttatt tttccgacag gtttataaat tttgacatag ttttcaaaga
     1321 taaagccaca gctaacataa tggacggcgg agttatctat cagggaaggg atttttcact
     1381 tgaacagttg aaaagctata cagaaaagat atgtggcgct tattcaagat caggagaagt
     1441 aaaatatgga aaaaaacttc cggttatttt ctcaagctat gatatgctcg ttatgatgaa
     1501 gtttatcact gatctgaacg gaagaatgta tggcagcggc ggatctattt tcagcaacaa
     1561 gataggcgaa aaactttttt cagaagagtt tacacttttc cagtccaatg atccttttaa
     1621 cagcatacct gactttgctt tttttgatgc tgaagg
//
LOCUS       DAOSHR010000345         1643 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_76424_length_1643_cov_3.455290, whole genome shotgun sequence.
ACCESSION   DAOSHR010000345 DAOSHR010000000
VERSION     DAOSHR010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76424_length_1643_cov_3.455290"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1112)
                     /locus_tag="PLU28_11455"
     CDS             complement(<1..1112)
                     /locus_tag="PLU28_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1957 domain-containing protein"
                     /protein_id="HOG35719.1"
                     /translation="MNNPKGYFSLMLHAHLPYVRHPDFEEFLEERWLFEAITETYIPL
                     IKMFKNLERDKVQFHLTMSITPPLMEMLGSDDLNQKYSRHMEKLIELCEKEVVRTKDE
                     DLRKHKMAKFYLADFKETYVLFYHDYNQRLLDAFREYQDKGYIEIVTCNGTHGFLPFM
                     QQYPSAIRAQMDAGVKTYERFMKRSPKGVWLAECAYFPGAEKYLSEYGINYFFTDSHA
                     LWFADEPAHYGVYRPVMTDEGVFAFARDPESSEQVWSATVGYPGDFRYREFYRDIGFD
                     REIDYIKPYIDRSGVRCNVGIKYHKISGKDVEIDRKDYYDIDEAKQAVKEHATDFLNK
                     KIEQTKRLYGIFDGEVPLITAPFDAELFGHWWYEGP"
     gene            complement(1109..>1643)
                     /locus_tag="PLU28_11460"
     CDS             complement(1109..>1643)
                     /locus_tag="PLU28_11460"
                     /inference="COORDINATES: protein motif:HMM:NF027583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4912 domain-containing protein"
                     /protein_id="HOG35720.1"
                     /translation="TELCETYNNDRLVIMVVNPFWIFTYWDFSKELSSHLKKIPHADS
                     FFMRVYDITNIIFDGSNANRILQKSIKIFEKSGAYFNVPMPDADYISEIGYYENNRFI
                     AVLKSNIVHTPPSSPRNVKFQKWVNLKDKTVKQIILDENYRPLITVIEKLIGPENAFS
                     SGSAFRTSEISKSGGIK"
BASE COUNT          478 a          342 c          278 g          545 t
ORIGIN      
        1 aacggtcctt cataccacca gtgtccgaaa agttccgcat caaaaggggc agttataagc
       61 ggcacttctc catcaaaaat accgtaaagt cttttggtct gttctatttt cttattgaga
      121 aaatcagttg catgttcctt tactgcctgt ttcgcttcgt ctatatcgta atagtccttt
      181 cgatcgatct ctacatcttt tccggaaatt ttatgatact ttattccaac attgcacctt
      241 actccgcttc tgtctatata tggctttatg taatcaatct ccctgtcaaa gcctatatcc
      301 ctgtagaact ctctgtatct gaaatctccg ggatatccga ccgtcgcact ccatacctgt
      361 tcacttgact ccggatcacg tgcgaaagca aaaacccctt catcagtcat tactggccta
      421 tatactccgt aatgcgcagg ttcatccgca aaccacaagg catgtgagtc agtaaaaaag
      481 taatttattc catactccga aagatacttt tcggctccgg gaaagtaagc acattcagcc
      541 agccatactc cctttgggct acgtttcata aagcgttcgt aagttttcac acctgcatcc
      601 atctgggctc ttatagcaga aggatactgt tgcataaaag gtagaaagcc atgtgttccg
      661 ttacaggtaa ctatttctat atatcctttg tcctgatact ctctgaatgc atcaagtaac
      721 ctttgattat aatcatgata aaataataca taagtttcct taaaatcagc taaataaaat
      781 ttggccatct tatgtttacg aagatcctca tcttttgtcc ttacaacttc tttttcacaa
      841 agttctataa gcttttccat gtgtctggaa tacttctggt tgagatcatc tgatcccagc
      901 atctccataa gaggaggagt tatactcatg gtaagatgaa actgcacttt atctctttca
      961 agattcttaa acatttttat caagggaata tatgtttcag ttatagcttc aaaaagccat
     1021 ctttcttcca gaaattcttc aaaatccgga tgtcggacat aaggaagatg agcgtgaagc
     1081 atcagtgaaa aataaccttt aggattattc attttatacc tcccgatttt gagatttctg
     1141 aggttctgaa agcggagccg ctggaaaaag cattctccgg acctataagt ttttcgatta
     1201 cggttatcag cggacggtaa ttttcgtcta aaattatttg tttgacggtt ttatctttta
     1261 gattaaccca tttttggaac ttaacgtttc tgggtgaaga aggcggagta tgcactatat
     1321 ttgatttcag aactgctata aacctgttgt tttcatagta tcctatctcc gaaatataat
     1381 ccgcatcagg cataggaaca ttaaaatatg cacctgactt ttcgaatatc tttatactct
     1441 tctgaagtat tctgtttgca ttactgccat cgaaaataat atttgtaata tcatatactc
     1501 tcataaaaaa ggaatcggca tgaggaattt tttttagatg agaagaaagt tcttttgaaa
     1561 aatcccagta agtgaaaatc cagaaaggat taaccaccat aataacaagt ctgtcattgt
     1621 tgtaggtctc gcatagttcg gtg
//
LOCUS       DAOSHR010000346         1616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_78374_length_1616_cov_4.498398, whole genome shotgun sequence.
ACCESSION   DAOSHR010000346 DAOSHR010000000
VERSION     DAOSHR010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78374_length_1616_cov_4.498398"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(47..178)
                     /locus_tag="PLU28_11465"
     CDS             complement(47..178)
                     /locus_tag="PLU28_11465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35721.1"
                     /translation="MKKLLVVLIALCAATAVFAEETATKLNVGANGFVFGNNYKQIV"
     gene            352..1587
                     /locus_tag="PLU28_11470"
     CDS             352..1587
                     /locus_tag="PLU28_11470"
                     /inference="COORDINATES: protein motif:HMM:NF013752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOG35722.1"
                     /translation="MFKIVRFPSKLENFFSSLKTEFLFGHFEYFRILVLLIAISWEDS
                     NISSLCRHLDQRFFTHRTRYNNFLTSVRWNPEQVLARKSYEILRTLKLKKGEPIYILV
                     DDTKKEKRGKTMDAAGWVYDPVTGRAVWGHQYVKATLLVRGITIPFAIRLYVKDKDCD
                     ELSVPFFKVTELAADMISSFNAPAGSKVTVLFDSYYLCPAVINACRKKGFYFISTLKS
                     NRNLKKRGKKLKSGIYGQYCFKTFNKLKMKIRKEHGIVTYTYVDAGFIDVSKIGKAHV
                     VFSRKNKEKQIIALVTDDPKLKASEIIKTYSLRWHIEVFFKDSKQLLGLGRYQNRSYK
                     AAVTHLHLVSFAYALLTHIAITGTCAKENKNNKQRQSVRYLQNAVRTIIWDDTALYLK
                     ELPNENSVFKELRHLLVAA"
BASE COUNT          549 a          288 c          293 g          486 t
ORIGIN      
        1 taagaagggg ctgaatacgt gcttcaccga aatcaccatt tttgtctcaa actatctgtt
       61 tataattatt accgaataca aaaccgtttg caccaacatt aagttttgta gctgtctctt
      121 ctgcgaaaac tgctgttgca gcgcagagtg caataagaac tactaataac ttcttcattg
      181 atctctccta aatatttaat ttttattaga gatagtaaca aatattatat tatttaactt
      241 gactgacttt taagtaaaga tataagatta aagagggaaa aggagaataa aaaaaaggca
      301 aaacagccga tgaaataggt aaccacaccg aagttcacag gagtttgcct tttgtttaag
      361 atcgtccgat tcccctccaa actcgagaat ttttttagct cattgaaaac tgaattttta
      421 tttggtcact ttgaatattt ccggatactt gttcttctga tagctataag ctgggaagat
      481 tcaaatattt catctctttg ccggcatctt gatcaaaggt ttttcaccca tagaactcgc
      541 tataataact ttctgacttc agttcgctgg aatccggaac aagtattagc caggaagtca
      601 tacgaaatac tgagaactct aaaacttaaa aaaggtgaac ctatatatat acttgttgat
      661 gataccaaaa aagagaaacg tggtaaaaca atggatgcag ccggttgggt atatgatcct
      721 gttaccggca gagccgtatg gggccaccag tatgtcaagg caacactgct tgttcgtggt
      781 ataacaattc ccttcgctat acgtttatac gtgaaagata aagactgtga tgaattgtca
      841 gtcccttttt ttaaagtgac agaacttgca gcagatatga tttcatcatt taacgctccg
      901 gcaggaagca aggtaacagt tctttttgac tcatactatc tgtgcccggc agttataaat
      961 gcctgtcgga aaaagggatt ttatttcata tccactctta aatctaatag aaatctcaaa
     1021 aagagaggta aaaagcttaa atccggcatt tatggtcaat actgtttcaa aacttttaat
     1081 aagcttaaaa tgaaaatcag gaaagagcat ggaattgtta catataccta tgtagatgca
     1141 ggttttatag atgtaagcaa aataggtaaa gctcatgtag tcttttcaag aaaaaataaa
     1201 gagaaacaaa tcattgcttt agtgactgat gatccgaagc ttaaagcttc tgaaataata
     1261 aagacttatt cactgcgctg gcatatagaa gtattcttca aggattccaa acagctttta
     1321 gggttagggc gttatcagaa ccgttcgtat aaagctgcag ttacacacct gcatcttgta
     1381 tcatttgcct atgcccttct gacccacatt gctattacag gcacatgtgc aaaagaaaat
     1441 aagaacaata agcaacggca atctgtaaga tatcttcaaa atgcagtcag aaccattatc
     1501 tgggatgata ccgctttata tcttaaagaa ttaccaaatg aaaattcagt tttcaaagaa
     1561 cttcgccatc ttctggtggc ggcttaaatg attaaaaagt cagtcaagat gagtaa
//
LOCUS       DAOSHR010000347         1615 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_78442_length_1615_cov_7.025000, whole genome shotgun sequence.
ACCESSION   DAOSHR010000347 DAOSHR010000000
VERSION     DAOSHR010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78442_length_1615_cov_7.025000"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="PLU28_11475"
     CDS             <1..438
                     /locus_tag="PLU28_11475"
                     /inference="COORDINATES: protein motif:HMM:NF015563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HOG35723.1"
                     /translation="AVLILMGISVKHIIVSGNIMDTLLYYAAQPLSGASPYSAVIIIY
                     MIVLCSNFFIGSASAKAFLLIPLLVPFAQMVGLSNQIMITAWAFGDGFSNALYPTNPV
                     LLICLGLSTVSYPKWFRWTFLLQLIVFIMTIGFSLFAVFINYV"
     gene            449..>1615
                     /locus_tag="PLU28_11480"
     CDS             449..>1615
                     /locus_tag="PLU28_11480"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOG35724.1"
                     /translation="MEKEYPQVVKKLSEAVRFKTVSYQESQNIDYGEFDRFQYFLQSA
                     FPNVHEKLKKEIINGYGLLFHWESGKQSKPVLLMAHQDVVPIEGDTEKDWKYPPFSGA
                     VEDGFVWGRGTLDIKVVLICVMQAVEKLLSEGYEPSRDIYLAFGHDEEITGENGAGMI
                     SSHLKNKGIEFEYILDEGGNIMQGVLPLLDKPVATVGVAEKGFLSLCLSVKAAGGHSS
                     MPGEDTCIRILSTALGKLRHTLFKPYLKGPTSMFFSKVAPDIKGFLGFVFRNQAVFSP
                     LLKKVLSNSPTTNALIRTTIAPTMFHAGDKENVLPQNASAVINLRIIPQESVEFCIEK
                     IKNTVNDNRVEVKILDGSRADNPSEVTSVESFGYKALEKVMEKVFPGVITAPFIM"
BASE COUNT          451 a          270 c          367 g          527 t
ORIGIN      
        1 gctgttctta ttcttatggg tataagcgta aagcatataa ttgtaagcgg caacataatg
       61 gatactcttt tatattatgc tgctcagccg ctttccggag catctcctta ttcggcggtt
      121 ataataatat atatgatagt cctgtgttct aactttttca taggttccgc atcagctaag
      181 gctttccttc ttattccact tcttgtgcct tttgctcaga tggtgggtct ttccaatcag
      241 atcatgataa cggcttgggc ttttggcgat ggattcagca atgcattgta tcctaccaat
      301 cctgtgcttc tcatctgttt gggtctttct actgtaagct atcccaaatg gttcagatgg
      361 acttttttac ttcaattaat tgtgtttatt atgacgatag gcttttcatt atttgcagta
      421 ttcataaatt acgtataggg ggaactgtat ggaaaaagag tatccgcagg tggttaaaaa
      481 actttccgaa gcggtaaggt tcaagacggt atcttatcag gagagtcaga atattgatta
      541 tggcgaattt gacagatttc agtactttct tcagagtgct tttcctaatg ttcatgagaa
      601 actcaaaaaa gagataataa acgggtatgg tcttttattt cattgggaaa gcggtaagca
      661 atctaaaccg gtgttgctta tggcgcatca ggatgttgtt ccgatagaag gagataccga
      721 aaaggattgg aagtatcctc cgttctccgg tgcggttgag gatggatttg tctggggaag
      781 aggtactctg gatataaaag tggtgctcat atgtgttatg caggcggtag aaaaactttt
      841 atcagaaggt tatgaacctt caagggatat atatctggca tttggacatg atgaggagat
      901 aaccggagaa aatggtgccg gaatgatttc ttcacatctg aaaaataagg gtatagagtt
      961 tgaatatatt ctggatgagg gcggaaatat tatgcagggt gttcttcctc ttctggataa
     1021 accagtggct acggtagggg ttgccgaaaa aggttttttg agtctttgtc ttagtgtaaa
     1081 ggctgccggt gggcactctt caatgccagg cgaagatacc tgtataagga ttttatcaac
     1141 tgctcttggt aagcttaggc acacactttt taaaccttat cttaaagggc cgacatcaat
     1201 gtttttttct aaagtggctc cagatataaa aggtttttta ggctttgttt tcagaaatca
     1261 agctgttttt tctccgttac ttaaaaaagt gctatcgaac tctcctacga ccaatgctct
     1321 tattaggaca acgattgctc ccaccatgtt tcatgcgggg gacaaagaaa atgttcttcc
     1381 tcagaatgct tctgctgtta ttaatttgag gataattccg caggaaagtg ttgaattttg
     1441 cattgaaaaa ataaaaaaca ctgttaacga taatagggtc gaagtaaaga tacttgatgg
     1501 atccagagcg gacaatcctt cggaagtaac ttctgtagag tctttcgggt ataaagcatt
     1561 agaaaaggtt atggaaaaag tatttcccgg agtaataact gctccgttta taatg
//
LOCUS       DAOSHR010000348         1595 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_79972_length_1595_cov_3.729870, whole genome shotgun sequence.
ACCESSION   DAOSHR010000348 DAOSHR010000000
VERSION     DAOSHR010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79972_length_1595_cov_3.729870"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..902)
                     /locus_tag="PLU28_11485"
     CDS             complement(<1..902)
                     /locus_tag="PLU28_11485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35725.1"
                     /translation="MGTSKNKGTSGAQGQVKISCGSSASRSVSLLCFILTAALLFAIP
                     RVGLVYEGPQSVLNSLIYTLYKYADIQIPPDYKGETFVIEADNDKFSLKYSLGQIQAG
                     TQEFDSIFSKIFPQERRSVFIIGKECTIKSEDKFRSADFFYWEEKLNLTIQASSGVYD
                     YVMYPRIGDNVLYVPKRWIKLFVTAEKVSEITLNGQRYLVPCEVTVPDCKLTLDAGDD
                     SFEIDLTNYEGDEYRFEIAQTDQSDIIKTAVMRVFEIESGIFISGFPYSVWLSKGQEP
                     KISKSQFFCGYGDIRDVKNGAELLG"
     gene            complement(797..1534)
                     /gene="truA"
                     /locus_tag="PLU28_11490"
     CDS             complement(797..1534)
                     /gene="truA"
                     /locus_tag="PLU28_11490"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOG35726.1"
                     /translation="MRRVAIKLAYEGTEFFGYQGQRTKRTVQDELEKGLQKIFGFRIN
                     TFVAGRTDTGVHAYGQVVAFDVPNDRMTFTNIKDALNSMIPHDMYVKDVWEVPCTFNP
                     RVTAVKRVYHYYIYSAPDPDIFFRNRVWWFPYELDLSSMRQAAAFFIGEHDYTTFKSG
                     DDERSVIRTVFRVRILRQSNNIILIRVEGKSFLRRMVRNMVGALVKVGTGSWEPQKIR
                     ELLELKDRSKSPAAAPPQGLYLYSVLF"
BASE COUNT          471 a          364 c          273 g          487 t
ORIGIN      
        1 cctagaagct cagccccgtt tttcacatca cgtatatcac cgtagccaca aaaaaactgt
       61 gatttgctta tcttaggctc ctggccttta ctaagccaga cggagtatgg aaaaccggaa
      121 ataaaaatac cgctttctat ctcaaaaact ctcataacgg cagtctttat tatatctgac
      181 tgatctgttt gggcaatctc aaaacggtat tcgtctcctt catagttggt aagatctatt
      241 tcaaaactgt catcacctgc atcaagagtc agtttacagt caggtacggt tacttcacat
      301 ggaacaaggt acctctgacc attaagagta atttctgata ctttttctgc agttacaaaa
      361 agttttatcc atcttttggg tacatacagg acattatcac caattctggg atacatgacg
      421 tagtcataaa cccccgaaga tgcctggatt gtcagattca gtttttcctc ccaataaaaa
      481 aaatcggcac tcctgaactt atcttctgat tttattgtac actctttgcc tattatgaat
      541 acagaccttc tttcctgcgg aaaaattttg gaaaaaattg aatcaaactc ttgtgttccg
      601 gcttgaatct ggccaagaga gtattttaaa gaaaatttat cattgtcagc ttcaataaca
      661 aaagtttccc ctttgtaatc cggaggaatc tgtatatcag catatttata tagtgtatat
      721 atcagactgt taagaacaga ctgcggtcct tcataaacaa gacctactct gggtattgca
      781 aacagcagag ccgctgtcag aataaaacag agtaaagata cagaccttga ggcggagctg
      841 ccgcaggaga ttttgacctg tccttgagct ccagaagttc ccttattttt tgaggttccc
      901 aactgccagt acctaccttc accaatgcgc ccaccatgtt tcgcaccata cgtctgagga
      961 atgactttcc ctcgactctt atcagtatta tattattact ctgtcttaag atacgtacac
     1021 gaaagacggt tctgattaca gatctttcgt catcaccgct tttaaaagtg gtgtaatcat
     1081 gctcacctat aaaaaaagca gctgcctgac gcatacttga aagatccagt tcataaggaa
     1141 accaccatac tctgtttcga aaaaatatat caggatccgg agcgctgtag atgtagtaat
     1201 gatataccct ttttacggct gtgactctgg ggttaaaagt acatggaacc tcccagacat
     1261 cttttacata catatcatgg ggtatcatgg aattaagcgc atcctttata ttagtaaaag
     1321 tcattctgtc gttcggaaca tcaaaagcaa caacctgacc gtaagcatgg actcctgtat
     1381 cggttctgcc tgccacaaaa gtatttatcc tgaaaccgaa aatcttctga agtccttttt
     1441 caagctcatc ctgaacagta cgttttgttc tttgcccctg atatccgaaa aactctgtac
     1501 cctcgtaagc tagttttatg gcaactcttc tcacaactct aaccctccgc aatattttga
     1561 ataagacttg tttccattat cttgtacgaa atctc
//
LOCUS       DAOSHR010000349         1581 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_81073_length_1581_cov_5.639581, whole genome shotgun sequence.
ACCESSION   DAOSHR010000349 DAOSHR010000000
VERSION     DAOSHR010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81073_length_1581_cov_5.639581"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1542)
                     /locus_tag="PLU28_11495"
     CDS             complement(<1..1542)
                     /locus_tag="PLU28_11495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35727.1"
                     /translation="MLASARIVLPLVVGDHKIKIEVSDKANQTTVLNQEYTIRPEGVL
                     TTPELMYYAPVFVEPGERFVITLSASDSINRIKQIELSDNFSGNPYVLSIPSEAFYEK
                     TITRIAPTREGEYFFTAKALNVAEVQKESSFKIIVQKNQVPRVSLSVSNASPTVNEKI
                     NISVDAWDDIGLSLVEVFDNGDSIAKYGTGSFKMVEDIGWVLKATHVWSANKGRHTFT
                     AKVTDKRGLTSEFILKQKIQVEDTTPPIVDNLFIDVRVDRDPNNLRTLKTHPTFNIKK
                     NDRIYLSFRVTDPESEVKSVTYTLKPDGLLPTKEYTTVSDDGYIFNTALEDYYDLPDM
                     VSPNNKIYITVTTENIGGYKKTYENFATIFVSPDKDVYKPKISLRIDPAKGYIFNSIN
                     ILPSYSDEGYIKNISLAIYKLNQSNEPIKISDGQQTELNMPANPDGSLDLTKSLNTKE
                     IPENKVKLTWLASDYGRFIAVARAENDLGIQNSQSIVFDVQGVSLLIRNPTEAKVIDL
                     GRNDLE"
BASE COUNT          436 a          331 c          275 g          539 t
ORIGIN      
        1 ctcaagatca ttcctgccaa gatctattac cttcgcttct gttggatttc ttattagaag
       61 ggaaacaccc tgaacatcga aaactatact ttgggagttt tgaattccaa gatcattttc
      121 tgctctggct acagcaataa accttccgta atcagatgca agccatgtga gctttacttt
      181 attctccgga atttcctttg tattaagtga tttggttaga tcaagcgaac cgtctggatt
      241 tgcaggcata ttcagttccg tctgctgtcc gtcggatatc tttatgggct cattgctctg
      301 gttaagctta tatatggcaa gagatatatt ttttatatat ccttcgtctg agtaagaagg
      361 cagaatattt atgctattga atatgtatcc ttttgcaggg tctattctaa gactgatttt
      421 tggtttgtaa acatctttat caggagatac aaatatagtt gcaaagtttt cataggtttt
      481 tttgtatcct ccgatatttt cagttgtcac tgttatataa atcttattgt ttggagaaac
      541 catatcggga agatcataat agtcctccaa cgcagtatta aaaatataac catcatccga
      601 aaccgttgta tactcttttg taggaagtaa gccatcaggt tttagagtgt acgtaaccga
      661 tttaacctcg ctttcagggt ctgtaaccct gaaactcaga tatatcctgt cattcttttt
      721 tatattgaag gtagggtgtg tcttgagagt ccttagatta ttaggatctc tgtctaccct
      781 tacatctata aaaagattgt ctactatagg aggtgtggta tcctctacct ggatcttctg
      841 cttaagtatg aactctgaag taagacctct cttatcggta acctttgcag taaaagtatg
      901 tcttccttta tttgcagacc agacatgtgt ggcttttaaa acccatccga tatcttcaac
      961 cattttaaag ctgccggttc cgtacttagc tatactatcg ccgttatcga atacctcaac
     1021 cagactcaat ccaatatcgt cccaagcatc aacgcttata tttatctttt catttactgt
     1081 cggagaagcg ttgcttaccg aaagacttac tctaggtacc tgattcttct gaactattat
     1141 cttaaaagag ctttccttct gaacctcagc tacattaagc gctttggcag tgaaaaaata
     1201 ctctccttcc cttgtaggag caattctggt tatagtcttt tcataaaatg cctccgaagg
     1261 aatgctcaga acataaggat taccactgaa attatcactc agctctatct gctttattcg
     1321 gtttatgctg tcagaagcag acaaggtaat gacaaacctt tcacccggtt ctacaaaaac
     1381 aggtgcataa tacataagct ccggagtagt cagaacacct tccggcctta ttgtatattc
     1441 ctggtttaat acagttgttt gattagcttt atcagaaacc tctattttaa ttttatgatc
     1501 ccctaccact aaaggcagga ctattcttgc actggcaagc atccaagaag ccgtttcctc
     1561 ctgaatcagc acataatcat c
//
LOCUS       DAOSHR010000350         1579 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_81233_length_1579_cov_7.528215, whole genome shotgun sequence.
ACCESSION   DAOSHR010000350 DAOSHR010000000
VERSION     DAOSHR010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81233_length_1579_cov_7.528215"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..493)
                     /locus_tag="PLU28_11500"
     CDS             complement(56..493)
                     /locus_tag="PLU28_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOG35728.1"
                     /translation="MKGDAEMVDVLSFEINNQEFAVEVEYVEIVYDMVEIRPVPNSKK
                     FIEGLINLRGRIVPVVDITKILNVSLGQEHKFSNILILKINEEEIGIFVDEVKNVLSV
                     DQNTLENISSKNEIYRDKSKGVIKINNRLIVFLNITELMSMQA"
     gene            complement(490..1491)
                     /locus_tag="PLU28_11505"
     CDS             complement(490..1491)
                     /locus_tag="PLU28_11505"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOG35729.1"
                     /translation="MNEKDSIRVDTAKLDELMNLMAELVIAKSRILDTLRKYNIKEVD
                     ESLSQLSRITLDLQNVVMKVRMVNVETVFSKFPRIMRDLEKQSAKEIDFIMQGTETEL
                     DRTVSEEISEPILELLNFSIQDLEDPNERITKGKNKKCSIRLSAKYEGNGVVISIQSD
                     GRGVIPQEISGPRQKIENLKGSLSTESQKGSWMRFNIRLPLTLSIMRALLVNIGKYVY
                     AVPIENINTTQKLVKGDIKFVQDREVFMLRGEVIPIVRPHKIFGEEDSGYDENKNIVI
                     VKFGDKKYGIIVDVLLGQDDIVIKPLGKLLSDVDTFNGGAILGDGSIALILDLGALS"
BASE COUNT          405 a          361 c          229 g          584 t
ORIGIN      
        1 agaacaaata taggcaggag tcttttatac catagtattt ttttcagcat acttttcagg
       61 cttgcatact catcagttct gttatattta aaaatacaat gagcctgttg tttattttta
      121 tgactccctt tgacttatcc ctatatattt cattctttga agatatattt tcaagagtgt
      181 tttggtcaac agaaagaaca ttttttactt cgtcgacaaa aatacctatc tcttcttcat
      241 ttatttttag tatcagtata tttgagaact tatgctcctg ccctagcgaa acattaagta
      301 tctttgttat atcaacaaca ggaactatcc ttcctctgag attgataagc ccttctatga
      361 atttttttga gttgggaaca ggtcttatct ctaccatatc gtatactatt tcaacatact
      421 ccacttcaac tgcaaactcc tgattgttaa tctcaaaaga taatacatca accatttctg
      481 catctccttt catgaaagtg ctcccaggtc caatatgagt gctatcgatc catctcccag
      541 tattgctccg ccgttgaagg tatcaacatc gcttagaagc tttcccagag gttttatgac
      601 tatatcgtcc tgccctaaaa gcacatctac gattattccg tattttttat ctccaaattt
      661 tacgattact atatttttgt tttcatcata cccggaatcc tcctcaccga aaatcttatg
      721 aggtctgaca atgggtatta cttcccccct gagcataaaa acctctctgt cctggacaaa
      781 ctttatatct cctttcacaa gcttttgggt agtatttata ttttctatcg gaactgcata
      841 gacatacttt ccgatattga caagcagtgc cctcattatt gagagtgtca gcggcagacg
      901 tatattgaac ctcatccatg aacccttctg gctttcagta ctgagactgc ccttaaggtt
      961 ttctattttc tgtctcggac cgcttatttc ctgggggatc acccctcttc cgtcgctctg
     1021 tatgcttata accacaccgt ttccctcata ctttgcgcta agccttattg agcatttttt
     1081 gttcttgcct ttggtgattc tttcattggg atcctcaaga tcctgaatac tgaagttcaa
     1141 aagttccagt ataggctcac ttatctcttc ggatacggtt ctgtcaagct cggtttctgt
     1201 tccctgcatt atgaaatcta tttcctttgc gctttgtttt tccaaatctc tcattatccg
     1261 tgggaactta ctgaatacag tttcaacgtt taccattctt accttcataa ccacattctg
     1321 aagatccaaa gtaattctgg aaagctggga aagactttca tcaacttcct ttatgttgta
     1381 ctttcttaaa gtgtcgagta tcctgctttt ggctataaca agttcagcca tcagattcat
     1441 gagctcgtcc aattttgcgg tatctactct tatggaatct ttttcattca taggcttcct
     1501 cctcttcaac tttaactttc ttcagaatcc gtttcatcaa attaacatta gcacttatat
     1561 tatatcacta ttattataa
//
LOCUS       DAOSHR010000351         1578 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_81319_length_1578_cov_3.655286, whole genome shotgun sequence.
ACCESSION   DAOSHR010000351 DAOSHR010000000
VERSION     DAOSHR010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81319_length_1578_cov_3.655286"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="PLU28_11510"
     CDS             <1..246
                     /locus_tag="PLU28_11510"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOG35730.1"
                     /translation="EYLKKKLEPLTCENIAEVRGMGLMLGAVMEKPVENLLKKGVDNF
                     ILLNLVRKSIIRLLPALNITFQQIDEMAEKLLIVLKE"
     gene            complement(330..839)
                     /locus_tag="PLU28_11515"
     CDS             complement(330..839)
                     /locus_tag="PLU28_11515"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOG35731.1"
                     /translation="MKNFIVIRSANEHDMDRILEIQSKCFRQGYLESRDSLFSKILSS
                     GDTCILAIAGTEIAGYFFSVEADFYEPPVFNSTRHILKRTPDCLYLHDLSVDPKFRGL
                     GIGNLLLERLVEKAKEKALNRLSLIAIDGLVPYWTKNGFSVVTAIPQKLSDCLQSYGK
                     SCTYMLRII"
     gene            932..1510
                     /locus_tag="PLU28_11520"
     CDS             932..1510
                     /locus_tag="PLU28_11520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35732.1"
                     /translation="MKKLFLSVLVVVLISIAAFSARIHGGISMLFFYPEVGITFNDDQ
                     NFYFDVSGYGGKYETVVNSITVPKDELSQIILSQGYYGSVRMEPGFGTELFSLGFLST
                     RFEIGPWVEANYYYSTLKNTSEKIIGLGGLNTKVYADINLSDNLSVGAWAGCYLLSAT
                     YSYDCITKESIFYLTPYLAAPVPAFGVSLSIN"
BASE COUNT          489 a          272 c          321 g          496 t
ORIGIN      
        1 gaatatctaa agaaaaagct tgagcctttg acctgtgaaa atatagcgga agtaagggga
       61 atgggcctta tgctgggagc tgttatggaa aaaccggtgg aaaacctcct gaaaaaagga
      121 gttgataatt ttattcttct aaatctggta aggaaaagta taatccgttt acttccggct
      181 cttaacatta cttttcaaca gattgacgag atggcagaaa aacttttgat tgtgcttaaa
      241 gagtagaccc tcataataaa ctgacctcct tttcgttggg ttcttgtcca acttttaggg
      301 ggtcagttca tatcataagg ctgtttattt tatatgattc tgagcatata tgtacaggat
      361 tttccgtagc tttgcagaca gtctgaaagc ttttgaggta tagcagttac aacggaaaaa
      421 ccgtttttgg tccaatatgg aaccagcccg tcaatggcaa ttaaagaaag cctattaaga
      481 gccttttctt ttgctttttc aaccagtctt tctaaaagaa ggttacctat tcccagtcct
      541 ctgaattttg gatctacaga cagatcatga agataaagac aatcaggtgt acgcttaaga
      601 atgtgccggg tggagttgaa aacaggaggt tcataaaaat cagcctctac agaaaaaaaa
      661 tagcccgcaa tttctgttcc ggctattgct agtatacagg tatctccgga gcttaagatt
      721 tttgaaaaaa gggagtcacg tgattcaagg taaccctgcc tgaaacattt agattgaatt
      781 tcaagtatcc tgtccatgtc atgctcattt gcgctgcgta tcacaataaa atttttcatg
      841 taagttatcc tccacagggc agaatctgat ataatcttta tatatactat tataacaaat
      901 atatacccta tatttaagga ggcagaaagt tatgaaaaaa ctctttttat cagtacttgt
      961 tgttgtatta ataagcattg ctgcattttc tgcgaggatt cacggcggta tttccatgtt
     1021 gtttttttat cctgaggtcg gtataacctt caacgacgat cagaactttt attttgatgt
     1081 cagcggatac ggcggaaagt atgaaaccgt tgttaatagc ataacggttc caaaggatga
     1141 gctttcacag ataatacttt cacaagggta ttacggtagt gtaagaatgg aacccggatt
     1201 tggaacagag ttattttctc ttggattttt aagcacacgg tttgaaatag ggccttgggt
     1261 tgaagcaaat tattactaca gcaccctaaa aaacacaagt gaaaagatta ttggtcttgg
     1321 agggctcaat acaaaagtat atgcagatat aaacctttca gataacttga gtgtaggagc
     1381 atgggcaggc tgctatcttc tgagtgcaac ctattcatat gactgtatta caaaagaatc
     1441 aattttctat ctgactcctt atctggctgc tccggttcct gcttttggag tgagtttatc
     1501 aataaactga taaaattctg ttctgttatg gtataatttt aacaccgaca aaaaaactcc
     1561 caattaagga ggtggtgt
//
LOCUS       DAOSHR010000352         1577 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_81397_length_1577_cov_3.565703, whole genome shotgun sequence.
ACCESSION   DAOSHR010000352 DAOSHR010000000
VERSION     DAOSHR010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81397_length_1577_cov_3.565703"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1563)
                     /locus_tag="PLU28_11525"
     CDS             complement(<1..1563)
                     /locus_tag="PLU28_11525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG35733.1"
                     /translation="MNEELSSTEEELLISHNRAIASEKRYRSIFNQAPVAMITLDKTL
                     HVTDYNMGAEKIFGWKKEEIIGIVFYKYIFTEHDISFFDNFLLDKSVKIPEYFVTENI
                     NKTGEIIICEWINSVIEDQKGNIAEIVSISRNITENKKLEESLRKAKDFAEKASKAKS
                     EFLANMSHEIRTPINGVIGISDLILETELDEEQKNYMQMIKTSSRNLLRIVNDILDIS
                     KIEAGKIELNNVSFHVLELKENIMNSFSHLMKEKGLDFKFFIQDSIPVKLNGDLYKIN
                     QILSNLVGNAYKFTDKGYVHVKTEKVHEDNTSVELLFNVSDTGIGIKPEKINIIFDIF
                     TQGDSSATKKYQGTGLGLTIAKSYVEKMGGKIFVVSTPGEGSSFSFVIPIKKDLSFNA
                     EKTQEDEEIYLQNYKDKKILVAEDNQINLYLTKKILSKKAMEVLTAENGAKAVEIFEK
                     ERPDLIFMDLQMPELDGLGATLKIRQIEKNHNTYTPIIALTAYATKEDKERCLAQGMD
                     GFIAKPFTQKDIF"
BASE COUNT          428 a          286 c          246 g          617 t
ORIGIN      
        1 aaatatatcc ttctgagtaa aaggttttgc aataaaaccg tccatacctt gagcaaggca
       61 gcgttccttg tcttctttgg tggcgtaggc tgtaagtgca attatagggg tataagtgtt
      121 atggtttttt tctatctgtc tgatttttaa agttgctccc aatccgtcaa gctctggcat
      181 ctgaagatcc ataaatataa ggtccgggcg ctctttttca aaaatctcaa ccgctttggc
      241 tccattttct gccgtaagca cttccattgc tttttttgaa agaatttttt ttgtcagata
      301 aaggtttatc tggttgtctt cagcgaccag aattttttta tccttgtagt tctgaagata
      361 tatttcttca tcttcctgag ttttttcagc attaaagctc aaatcttttt ttatgggtat
      421 aacgaaggaa aatgaacttc cttcacccgg tgtgcttaca acaaaaatct ttccgcccat
      481 cttttcaacg taacttttgg ctattgtaag tcccaaacct gttccttgat atttttttgt
      541 ggcggaagag tcaccctgtg taaaaatgtc aaatataata tttatctttt ccggctttat
      601 gcctatacca gtatcagaga cattaaataa tagttctacg gaagtgttat cctcatgcac
      661 tttttcagtt ttaacatgta catatccttt atcagtgaac ttataagcat tacctacaag
      721 gtttgaaagg atctggttta tcttataaag atctccgttg agctttacag gaatactgtc
      781 ttgtataaaa aatttaaaat caagtccttt ttctttcata agatgggaaa atgagttcat
      841 aatattttct tttagttcaa ggacgtggaa tgaaacattg ttaagctcta tttttcctgc
      901 ttcgatcttt gaaatatcaa gtatgtcgtt tactattctc aaaaggtttc ttgatgatgt
      961 ttttatcatc tgcatgtagt ttttttgttc ttcatcaagt tctgtttcaa gaatgaggtc
     1021 acttattcct atgactccat ttataggagt acgtatttcg tgactcatat tggcaagaaa
     1081 ctcacttttg gctttacttg ctttttctgc aaagtccttt gcttttctga gagattcttc
     1141 aagtttttta ttttctgtga tattccttga aatagatact atttcagcta tatttccctt
     1201 ctgatcttca atgacagagt ttatccattc acatattata atctctcctg ttttatttat
     1261 attttctgtg acaaagtatt ctggtatttt aacagactta tctaggagga aattgtcaaa
     1321 gaaagatata tcatgttcgg taaatatata cttataaaaa actataccta taatttcttc
     1381 tttcttccat ccaaaaattt tttctgctcc catattataa tcagttacat gaagagtttt
     1441 gtctaaagtg atcatagcca caggagcttg attaaaaatg ctcctatagc gcttttcact
     1501 tgcaattgct ctattatggg atatcagcag ttcttcctca gtacttgaaa gttcttcatt
     1561 caaagactcc agttcct
//
LOCUS       DAOSHR010000353         1576 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_81487_length_1576_cov_3.389875, whole genome shotgun sequence.
ACCESSION   DAOSHR010000353 DAOSHR010000000
VERSION     DAOSHR010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81487_length_1576_cov_3.389875"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="PLU28_11530"
     CDS             <1..419
                     /locus_tag="PLU28_11530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35734.1"
                     /translation="ICPLSYGGSKDYIQKTIDYGRTVFDDKIEFLLNFMPAKEYSNIL
                     EKTDIAVMNHNIQEGLGNIIALLYLGKKVFIRPRTTSFKFFNYMGVKLFSTDEIPSIS
                     FEAFSDILPDSQNSSLIYSFFNETNCIQLWKNLFLK"
     gene            complement(411..>1576)
                     /locus_tag="PLU28_11535"
     CDS             complement(411..>1576)
                     /locus_tag="PLU28_11535"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF015284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HOG35735.1"
                     /translation="KKHLGSEVENVYVEMSKNIYKNGIFVPIPGSRIGKGNMVAAALA
                     ACTDCENPDLTLLNSCTEQDNLKARKFLDEGKVQGKCLEDVNGVYVKAVVSSKDHQAC
                     CIVKNGHSDIEKIIVDDEVIFSKECKKGSEGFNFQGVNGLEDIVKISEEIDTQDMEFI
                     LYGAKMNYSVAQRYYLDSEMKLCSFFRKTGIENVGAKIRAYATIASYVRMSGINEKIM
                     SSGGSGNQGITVTLPVYIYAIESKADDRKTAVALCISHIFSGYIKSKIGKINAGCGAF
                     GAAGPASAAGIIYLMGGSIEQMKQAIITNLSNEAGVICDGAKESCAFRIGNAAYDAYY
                     SALMAANGVGVCSNQGVINTCLNSTIEDMKYLSDHTRDCIDDSLIEILENRSF"
BASE COUNT          432 a          369 c          247 g          528 t
ORIGIN      
        1 taatctgccc cctttcctac ggaggcagta aagattatat tcaaaagact atagattatg
       61 gaagaactgt tttcgacgat aagatagaat ttctgctcaa ctttatgcca gccaaagaat
      121 actccaatat tcttgagaaa actgacattg cagtaatgaa tcacaatatc caggaaggac
      181 tcggaaatat aatagccctg ttgtatcttg gaaaaaaagt ttttatacgc cctagaacta
      241 cttcatttaa gttctttaac tatatgggag ttaagctctt tagtaccgat gaaataccgt
      301 ccataagctt tgaagctttc tcggatattt taccggattc acagaacagt tctttaattt
      361 actctttttt taatgagacc aactgcatac agttgtggaa aaatcttttc ttaaaatgat
      421 ctattctcaa gtatctctat aagactgtca tctatacaat cacgtgtatg gtcactcaga
      481 tacttcatat cttctatagt tgagttaaga cacgtattta taactccttg gtttgaacat
      541 acgcctactc cgtttgcagc cataagtgca ctgtaatatg cgtcgtaagc ggcatttcct
      601 attctaaagg cacagctttc ctttgccccg tcacaaatta ctccggcctc gtttgagaga
      661 ttcgttatta tagcctgttt catctgctct atgctgccgc ccataagata aattattccg
      721 gcggcacttg caggacctgc tgctccaaat gcgccacagc ctgcatttat ctttccgatt
      781 ttcgacttta tatagccgct gaaaatatga cttatacaaa gtgcgaccgc agttttccta
      841 tcgtcagcct tactttcaat ggcatatata taaaccggta gcgtaacagt tattccctgg
      901 tttccgcttc ccccgctgct cattattttt tcgtttattc cgctcattct cacataagaa
      961 gctattgtag catatgccct tatctttgcc ccaacatttt ctatcccagt ctttctgaaa
     1021 aaactgcaaa gcttcatttc cgaatccaag tagtatcttt gggcaacaga gtagttcatc
     1081 tttgcaccgt aaaggataaa ctccatgtcc tgtgtgtcta tttcttcaga tatctttact
     1141 atatcttcaa gtccgtttac tccctgaaaa ttaaaccctt cagacccttt cttacactcc
     1201 tttgagaaaa ttacctcatc atcaacgatt attttttcaa tatcagagtg tccgtttttc
     1261 acaatacaac atgcttgatg atctttggag cttaccacag ccttcacata gactccgttg
     1321 acgtcttcaa gacatttacc ctgaaccttt ccttcatcca gaaactttct tgctttgagg
     1381 ttgtcttgtt cagtacagct gtttagcaat gtaagatcag gattttcaca gtccgtacag
     1441 gcagccagag ctgccgctac catattccct tttcctatac gtgaaccggg aatcggaaca
     1501 aaaataccgt ttttataaat gtttttgctc atttcaacat acacattctc aacttcagag
     1561 ccaagatgct tctttg
//
LOCUS       DAOSHR010000354         1557 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_83015_length_1557_cov_3.283622, whole genome shotgun sequence.
ACCESSION   DAOSHR010000354 DAOSHR010000000
VERSION     DAOSHR010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83015_length_1557_cov_3.283622"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..660)
                     /locus_tag="PLU28_11540"
     CDS             complement(79..660)
                     /locus_tag="PLU28_11540"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HOG35736.1"
                     /translation="MRFHFSPNLFVLYFSFFISLFLGVWGIKNRKKPAALQFGLSMVC
                     LTLWTFGFILELAGTDFKTKLIWYNFQFLFIYLFPVNLFWMTKDLSNLWLKEKLERFY
                     FYIFISISLIIFLTGVINPQDSLFMENLSIIENNGYTFLAGTAGPVYYIGIGYVFFFV
                     ILSVGILLYELIKREKFFKLQILLLLLAQIFPS"
     gene            complement(791..>1557)
                     /locus_tag="PLU28_11545"
     CDS             complement(791..>1557)
                     /locus_tag="PLU28_11545"
                     /inference="COORDINATES: protein motif:HMM:NF024947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HOG35737.1"
                     /translation="MQEQISTHEKELAISEKTVKILSEKIPNKSLSYEDLQKKSEELS
                     SVKREFEALSGELGTTDSKIKEMTEKLTEKTDMEKEYVLVKKEADTAELLSKKLRGNE
                     FVKYVSTLMMENILSAASSQLRAITNNRYSLETDESAFIVRDDFNGGYRRRVDTLSGG
                     EIFLASLCLALALSAHIQLKGKKSIRFFFIDEGFGSLDNSLIDTVMSSLEKIQQKHLC
                     VGLISHVEEMKERIQSKLIIYKQETENGITSKIDII"
BASE COUNT          477 a          279 c          287 g          514 t
ORIGIN      
        1 gtattccgga aagtgaaaag ataaacgtgc tgtagttaaa agaggtatca agcagcttaa
       61 aggtgtacag aacagaggtt atgagggaaa tatctgtgcc agaagcagca gaagtatctg
      121 aagcttgaag aacttttcgc gttttataag ttcataaaga agaatgccta cagaaagtat
      181 gacaaagaag aaaacatacc ctatgccgat atagtaaacc ggtcctgcag ttcctgcaag
      241 gaaggtgtat ccgttattct ctattatgga aagattttcc ataaaaagac tgtcctgtgg
      301 atttataact cctgtcagga agattatcag agatatgctt atgaagatat aaaaataaaa
      361 tcgttcaagt ttttctttaa gccaaaggtt tgacagatcc ttggtcatcc agaaaaggtt
      421 tacagggaat aggtatatga aaagaaactg aaagttatac caaataagct tggttttaaa
      481 atcagtacca gcaagctcca gaataaaacc gaatgtccac agagtaagac aaaccatgct
      541 aagcccaaat tgaagggcag ccggcttttt tctgtttttg ataccccata ctcccaaaaa
      601 aagagatata aagaaactaa agtaaagaac aaataggttc gggctgaaat gaaatctcat
      661 agcactccca gttatatatt ttttaaagta tatagttaat tataccatct catgagtgaa
      721 agaataaaaa caaattactt tatgtgtttt gcagcttatc tttttttata ttaacataag
      781 aaaaaaaagg ttatattata tctattttac ttgttattcc attttctgtt tcttgtttat
      841 aaataatcaa tttggattgt attctttcct tcatctcttc tacatgactt atgagtccga
      901 cgcacagatg cttttgctgt attttttcaa gagaagacat aacggtatca atgagactgt
      961 tgtccaaaga cccaaagcct tcatcaataa agaaaaatct gatgcttttt ttccctttaa
     1021 gctgtatgtg tgcggaaagg gcaagagcaa gacacaagga ggcaagaaag atctctccgc
     1081 cggataaggt atccaccctt cgtctgtatc ctccgttgaa atcatcacgt actatgaatg
     1141 ccgactcgtc tgtttcaagc gagtacctgt tgttggttat ggctctgagc tgtgaggaag
     1201 ccgctgaaag gatattttcc atcataagcg tagaaacgta ctttacaaac tcatttcctc
     1261 ttaatttttt gctgagcagt tcagccgtgt ctgcttcttt tttcaccagg acgtactctt
     1321 tttccatatc tgttttttca gtaagctttt ctgtcatttc ctttattttt gaatcggtcg
     1381 ttccgagctc tccgcttaaa gcttcaaact ctctttttac tgatgagagt tcttcactct
     1441 tcttttgcag gtcttcgtac gatagacttt tatttgggat tttttctgac aggattttta
     1501 ctgttttttc tgatatggca agctcttttt catgtgttga gatttgttcc tgcatct
//
LOCUS       DAOSHR010000355         1551 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_83541_length_1551_cov_3.939171, whole genome shotgun sequence.
ACCESSION   DAOSHR010000355 DAOSHR010000000
VERSION     DAOSHR010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83541_length_1551_cov_3.939171"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..856)
                     /locus_tag="PLU28_11550"
     CDS             complement(<1..856)
                     /locus_tag="PLU28_11550"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOG35738.1"
                     /translation="MDVYKGDIIYTAAPDSFTVLKNSFILVKDGIIQDIRQEVGPDLK
                     NIELHDYSGHLIIPCFIDMHLHSGQYMQKGLGMDKPLLQWLESYTFKEEAKFSKQEYA
                     QKVYESFCEELIENGSLKSVIFATVHKQSTEILFEKLIKKGLSAYVGKVNMDTNCPEE
                     IREEAADSLKDTEEIISKYSSQSMVKPIITPRFVPTCSRALLEELGKMALKYNVPVQS
                     HLSENREEVQWVKELFPDCQNYSRVYNSFGLFGQTPTVMAHCIYLTKDGKELIKNNGV
                     GIVHCPQSN"
     gene            complement(873..>1551)
                     /locus_tag="PLU28_11555"
     CDS             complement(873..>1551)
                     /locus_tag="PLU28_11555"
                     /inference="COORDINATES: protein motif:HMM:NF014373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HOG35739.1"
                     /translation="TIKEVEKLKHPFEFYIDITIRLIASTVAASIIGLTREKINRPAG
                     IRTHALIAIGSCIVTILSIYAFYDPITNTGDPGRLAAQIVSGIGFLGAGTILKRGMNV
                     KGLTTAAALWSVAAIGIAYGAGEYFIGTLGAALIFFIVTSNRFYRSNSNNIQLSVYLS
                     DSGAFKAVVDTIKKNNFNIKSIEKEVAEVGGYDFTFFLERKRSSNLQKLRDELLSIDG
                     VNFIEHN"
BASE COUNT          425 a          304 c          299 g          523 t
ORIGIN      
        1 agttagactg agggcagtgc actataccca ctccattatt ttttataagc tctttcccgt
       61 ctttggtcag atagatacag tgggccataa cggtaggagt ctgtccgaac agaccgaagc
      121 tgttgtatac tcttgaatag ttctgacagt caggaaaaag ttcttttacc cattgtactt
      181 cttctctgtt ttccgaaaga tgtgactgta caggaacgtt gtacttcaga gccattttgc
      241 caagttcctc caaaagtgct cgtgagcatg tgggtacaaa cctaggagtt attattggtt
      301 ttaccatact ctgggatgag tactttgaaa taatctcctc ggtatctttt aaagagtctg
      361 cagcttcttc acgtatttcc tcagggcagt tcgtgtccat gttaactttt cctacatatg
      421 ctgaaagacc tttttttata agcttttcaa aaagaatttc tgtagattgt ttatgaactg
      481 ttgcaaagat aactgattta agggatccgt tttcaatgag ttcttcgcaa aagctttcat
      541 atactttttg tgcatattcc tgttttgaaa actttgcttc ttctttgaag gtatagcttt
      601 caagccactg aagtaaaggc ttatccatgc caagaccttt ttgcatgtac tgtccgctgt
      661 gaaggtgcat atcaataaaa caagggatta taagatgtcc ggagtagtcg tgcagttcta
      721 tgtttttaag gtcaggacca acttcttggc gtatatcttg aattataccg tctttaacca
      781 gtatgaaact gttcttaagc acagtaaacg aatcaggtgc tgctgtatat attatatctc
      841 ctttataaac atccataaac agcctccgtt tttcagttgt gttctataaa gttcactccg
      901 tcaattgaaa gcagctcatc acggagtttt tgaagattag agcttctttt acgctccaga
      961 aaaaaagtaa aatcatatcc tcctacttct gccacttctt tttctattga ttttatattg
     1021 aaattatttt ttttgatggt atccacaact gctttgaatg caccgctgtc tgaaagatag
     1081 acactgagct gtatattatt tgagttagat ctgtaaaatc tgtttgatgt aactataaaa
     1141 aatattaatg ctgctccgag agttccgata aaatattccc cggccccata ggctatacct
     1201 atggcagcaa cagaccataa agcggcggca gtggtcaggc ctttaacgtt catgcctctt
     1261 tttaatatgg ttcctgcgcc aaggaaaccg attcctgaaa caatctgtgc agcaagacgt
     1321 ccgggatcgc cggtattggt aatcgggtca tagaatgcat atattgaaag tatggttact
     1381 atacatgatc ctatggcaat aagagcatga gttcttatgc ctgcaggacg gtttatcttt
     1441 tcccttgtca accctattat agaagcagca acggtagagg ctataagacg tatggttatg
     1501 tctatataaa attcaaaggg atgcttcaat ttttccactt cctttatggt g
//
LOCUS       DAOSHR010000356         1545 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrotogaceae bacterium isolate AMR_MDS_1722
            NODE_84053_length_1545_cov_4.032886, whole genome shotgun sequence.
ACCESSION   DAOSHR010000356 DAOSHR010000000
VERSION     DAOSHR010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863251
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrotogaceae bacterium (wastewater metagenome)
  ORGANISM  Petrotogaceae bacterium
            Bacteria; Thermotogota; Thermotogae; Petrotogales; Petrotogaceae.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,273
            Genes (coding)                    :: 2,268
            CDSs (with protein)               :: 2,268
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 3 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Petrotogaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84053_length_1545_cov_4.032886"
                     /isolate="AMR_MDS_1722"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819251"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="PLU28_11560"
     CDS             <1..353
                     /locus_tag="PLU28_11560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG35740.1"
                     /translation="IPFLLGTDGESSNQDLSVENEETFFTSQLDLPVKRLGQKIRFGS
                     AYVGSIEPGCFCDLKVKKNGKVTDVFIGGKRVFSNDTFVLPDSKNVEALEKDTSSVIT
                     SFFLYADKKIQEGN"
     gene            353..>1545
                     /locus_tag="PLU28_11565"
     CDS             353..>1545
                     /locus_tag="PLU28_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HOG35741.1"
                     /translation="MQKEFFIKKREQLFERLADDSVAILFSSNAPLKTADEHYAFTPN
                     RNFYYLTGLDRENFILVMKKKQGTASSSIYIEKHDPVLAKWVGDRMLADEVQSISGIS
                     DIRYVDDINKDIGSLLGGYDFENVYFDFEYRGWDRTCNEQRAFANELKSRFPSVSLRN
                     LYHIVSSMRLIKEPEEIEKIRQAISITQKGILNILANIRPGIMEYELEAYFDFTLKSC
                     GVKDFAFKTIAASGKNATVLHYSQNNSMMNENDLILFDLGAQFEYYNADISRTFPING
                     KYSQRQKDVYDAVLQAQQAVTQAAKPGVPFSVLNDTAKKVLAEGCRKLGLIKEDSELS
                     EYYYHGVSHYLGLDTHDVGTRDTVLKEGMVITNEPGLYIACEGIGIRIEDDLLISRDG
                     AEVLS"
BASE COUNT          524 a          269 c          316 g          436 t
ORIGIN      
        1 gaattccgtt cctattggga acagatggag agtccagcaa tcaagattta tcagtggaaa
       61 atgaggagac tttttttact tcacagcttg atctacctgt aaaacggctt ggacaaaaaa
      121 ttcgtttcgg tagcgcctac gtaggctcaa tcgaaccagg atgtttttgc gatcttaaag
      181 tcaaaaaaaa cggaaaggta acagacgttt ttatcggagg aaagagagta ttttcaaacg
      241 atacctttgt gttgcctgac agtaaaaatg ttgaagctct tgaaaaagat acatcttcag
      301 taataacaag ttttttcctt tatgctgaca aaaaaataca ggagggaaat taatgcagaa
      361 agaatttttt ataaaaaaac gtgaacagtt atttgaaaga ttagctgacg attcggttgc
      421 aatactgttt tcatcaaatg ctccactaaa aacagctgat gaacactatg cctttacccc
      481 aaacagaaac ttttattatt tgacaggtct tgacagggaa aactttatcc tggtaatgaa
      541 aaagaaacag ggaacagcat cttcttcaat atacatagaa aaacatgatc ccgtacttgc
      601 caaatgggtc ggagacagaa tgctcgccga cgaagtacag tctataagcg gaatttctga
      661 tattcgctat gtagatgata taaataaaga tataggttct cttcttggag gatatgattt
      721 tgaaaatgtg tattttgatt ttgaatacag aggatgggat cggacttgta acgaacagag
      781 agcatttgca aatgaactta aatcaagatt tccttctgta agtcttagaa atttgtacca
      841 tatcgtatcg tctatgagac ttataaaaga accagaagag attgagaaga taagacaagc
      901 tataagtatt actcaaaaag gaattctgaa tattcttgcc aacataagac cgggtatcat
      961 ggaatacgag cttgaagcat actttgactt tactttaaaa agctgtggtg taaaggattt
     1021 cgcatttaaa accatagccg ccagcggtaa gaacgcaaca gttcttcatt actcacagaa
     1081 taattccatg atgaacgaaa acgatcttat tctctttgat cttggcgcac agtttgaata
     1141 ttataatgcc gatatatcca gaacctttcc tataaacgga aagtactctc aaagacagaa
     1201 agacgtttat gatgctgttc ttcaggcaca gcaggctgta actcaggctg caaaaccagg
     1261 agtgcctttt tccgtactga acgatacggc aaaaaaagtt ttagcggaag gatgcagaaa
     1321 acttggactt ataaaagaag acagtgagct ttctgagtat tactatcacg gagtatctca
     1381 ctatcttggt cttgatacac atgatgtcgg tacacgcgat actgtcttga aagaaggcat
     1441 ggtcatcacc aacgaacctg gactttatat agcctgcgaa ggcatcggga tacgtattga
     1501 ggatgatctc cttatatctc gtgacggagc ggaggttctt tcaaa
//
