LOCUS       DAOSHV010000001        48579 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_558_length_48579_cov_5.996064, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000001 DAOSHV010000000
VERSION     DAOSHV010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 48579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 48579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48579
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_558_length_48579_cov_5.996064"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(124..1140)
                     /locus_tag="PKZ29_00005"
     CDS             complement(124..1140)
                     /locus_tag="PKZ29_00005"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOG37285.1"
                     /translation="MKKISFIIPTLNASLVLEKCLDSIKKQSIPSSDYEIVISDGGST
                     DNTINIAKKYNSIIVKNSLKTAEAGKAIGIKKASGKYICLIDSDNILPDNNWLNQMLF
                     PLEKDKKIIGSEPIEFTYRPAGGFIERYSSLIGANDPYAFVTGVSDRKNYIDNQWTRL
                     NIEQIDKDKYILVKLKPRQPIPTIGANGTIFRTEFLKNNLKSDYLFDIDIISQVINKN
                     KKPLYFAKVKQAIIHTFCESSVKKFIRKQNRRITDYYYYQKLRQYNWDQTNKFGTIKF
                     VLYTVLVFPSLFDSLRGFLNKPDVAWFFHPLACFITLYLYTINTIKYRLGLLKQLNRQ
                     QWQQ"
     gene            complement(1137..2642)
                     /locus_tag="PKZ29_00010"
     CDS             complement(1137..2642)
                     /locus_tag="PKZ29_00010"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG37286.1"
                     /translation="MVYPKKNQNLNHTPSTKKPVNTLVVKSVSFSYPPLESPKGTAFL
                     SQNRQFQWTNTGNVIYPIIPAYAATLLQSKGLKIYWDDAIAQKLTYQQWIDQLVKNKP
                     DLVVIETKTPVIKKHWEIINQIKQESLKIKNYKLKIVLMGDHVTALPQESIDNCPVDY
                     VLIGGHYDFLLNELIELINSGHAPKNKIYKLQKPINLNILPIIDRKLTHWQLYANNNT
                     NYKYKPGSYIMSGRDCWWGRCTFCSWTTLFPGKNFHSFSVDHTIKEIENLVNNFGVKE
                     IFDDSGTLPVGKWLNDLCSQLINRGLNKKVVLGCNMRFGVLSQKEYNLMKKAGFRFIL
                     YGLESANQKTLDFINKNEKTSSALKTLIMAKKAKLEPHVTVMIGYPKETLKQATKTLN
                     LARYIFQNNLADSLQATILIPYPGTPLFQYCQKNNLLLTTNWDDYDMRAPIIKSPIPP
                     KKQIELVQNLFKGVITPKFIFKKIISIRSFADIKHLSNYAFKYFKKLKDFS"
     gene            complement(2566..3549)
                     /locus_tag="PKZ29_00015"
     CDS             complement(2566..3549)
                     /locus_tag="PKZ29_00015"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOG37287.1"
                     /translation="MTKNILFSIIIPVRKINPYLRETLSHLKAQSLKNFEILVIDDKV
                     SGTANPSQKRNLGAKKARGTYLVFFDDDSFPSLNYFKNAAKLVKKHPDYAAFCGPYLT
                     PKKDNLFQQASGLVWSSYLGSGGAGTYRNRISSSRFVDDYPSVNLIIKKTDFNRAGKF
                     DPNYWPGEDTVLCLNLTKKLQQKIYYHPSLVVYHHRRQILIPHLQQITRYAVQRGYFV
                     KKFPQTSFRLGYFLPSLFLIYCLTLIVNLFTLNLKICYLPLYLYLSILFATFVKFIID
                     KNRPTTAFLAATAIPLTHFYYGILFLYGLSKKESKFKPHAVDKKTGKYLGG"
     gene            complement(3546..4349)
                     /locus_tag="PKZ29_00020"
     CDS             complement(3546..4349)
                     /locus_tag="PKZ29_00020"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOG37288.1"
                     /translation="MKSNRHFLSVIVPVYKQEKTITKDLTNILNVLDQIRYDYELIVV
                     VDGISVDNSYRLAKNIKSKNLTVTGYKNNHGKGYAVRYGMAKSKGDYVSFIDSGMEID
                     PNGISLILEHMEWYDADIIAASKHHPASQVKYPLNRKIISFGGHLIAKYLLGINIRDT
                     QAGLKIFKRKVLEKVLPRLLIKTYAFDLEIISVANHLGFTKIYEAPIKLNYDFGSLTH
                     AQGIKVIYQCLVDALAVFYRLRILHYYDDKNNRRWFYDPELSMRINTGQ"
     gene            complement(4339..5430)
                     /locus_tag="PKZ29_00025"
     CDS             complement(4339..5430)
                     /locus_tag="PKZ29_00025"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOG37289.1"
                     /translation="MPKKYHIAIDISPTVDGNSVRGVGYYTSNLIKSLKQHTQKHRSY
                     SNFNIDLITDRSKLDQKKYDLIHYPYFDPFKLTLPRSVKPTIVTVHDLIPIQFKNQFP
                     AGLRGRLKWQIQKRFLQKVDKIITVSHYSQKIISQIIKYPSENIFPIYEGADNSFKPI
                     TDPKILKTVKDKYSLPNKFILFVGDINWNKNIPNLVRACYRLKYPLVIVGSSAVKKDV
                     PNHPWTKDIRWLQARAKKYQKSKSLILTGFVSDEDLPVIYNLATVYCQPSFAEGFGLP
                     LVQAMQSGCPVAFSRDTSLPEVVGQAGLSFNPRSVPDISSTLKKYWLNPKLCRQYRQL
                     GLNRAKLFNWSDTAKQTLEVYQSVLIHEK"
     gene            complement(5430..6257)
                     /locus_tag="PKZ29_00030"
     CDS             complement(5430..6257)
                     /locus_tag="PKZ29_00030"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOG37290.1"
                     /translation="MKSKTATLSVTLATYNESKNIVRCLEAIKDIADEIIVVDGQSQD
                     DTVALVKKFKQARIISTTNKPMFHINKQIGISESKSDWILQLDADEVVTPKLKAEIKS
                     YLQKSPSSVPENGFWIPRKNYFLGTFLTKGGQYPDPTIRFYKNGVAHLPCRDVHEQAV
                     VKGTVGWLKNDLEHYADTSFSLYLLRANRYTTLLAEELKTVKTSLGFKGFITYYFFKP
                     LWWFLKTYFRHRGYVDGFPGFVFSFYSALRFPIAYTKYYELIKTNRDINLSQDWDKK"
     gene            complement(6254..7885)
                     /locus_tag="PKZ29_00035"
     CDS             complement(6254..7885)
                     /locus_tag="PKZ29_00035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOG37291.1"
                     /translation="MKIHKYLIYFILIVAFLVRFIGLDHTPALNPDEAALGYNAYSLI
                     QTGKDEHGVSWPLHFKSFGDYKPGGYVYLAIPFVKIFGLTPLAVRLPNLILSLLTIFF
                     LYKLILLISKSKNLSLYSSLVLAISPWHIHFSRGAWESQTALSLMVIGTYYFFKSIKS
                     SKFLSNFFLFVFFYVISLYFYHSLRLIAPLMALSLVIIYFKFLIKKLPLLIAPVIFGV
                     ILALPVLFSFLRHGGAARFGGVGLTADQGPIWRSNELLNQHQGTKLTNRIIHNQRLLY
                     LVSWAQKYTSHFDLNFLSINGDEVPRSKVPEMGQIYLIEVPLLFLGLIFLFKSKIYSP
                     KSRAFIISFLLISPIASSLTFQAPSALRALSMTIPLSILIALGIYSLFDSLKIFIHKL
                     YFKKLVSIFFVLIYLLSFAYYVDAYFFHYAKRYPFAWQYQFDQLVPYLESQKHNYQNI
                     YVTNKYDQPYILFLFFSHYPPSQIQSQIQLTPPDNFGFSTVLGYDNYHFGKINWDDIP
                     TGSLVVASDEDIPVNPDKIIYFSNQSPAFKIYQKI"
     gene            complement(7872..9506)
                     /locus_tag="PKZ29_00040"
     CDS             complement(7872..9506)
                     /locus_tag="PKZ29_00040"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOG37292.1"
                     /translation="MTKSVSNRLFLFLIFLISFLVRFVFWQKTPLLWDEAALGYNAYS
                     ISQTLKDEYGKFLPLIFKSFGDYKPGLYVYLALPFVKLLGLGELSVRLPSMIFGALSP
                     VFLFLLITQISPKSKKIASIAALICAFNPYNIHFSRGAWETNILTFELILASYFFYKY
                     INRQRSKYLFFSAIIFGLSLFTYQAGKMISLFLIIILFILNFKQIQFKKFLLSFVIPL
                     GIIALPVAYGLFFSSNANRLQVVSLFSYPRFEIETNTIINETNHLDYQIFHSQPSFFI
                     RNFLSRYFNHFSPRFLAFEGDWQNPRHSAPYIGVLLYPSLIFLVIGLVFGFSRSKNQS
                     INLFFLLWLLVAPIPSALTRDSIQSVRAMSLSIPLIYFISLGIFAFLKRYPSKLIPIF
                     IIFIYLISFVYYSDLYLNHMVKKSPYDFLYGYKQAVSYVNNSQSKYQQIIFSDFYGQP
                     YIFYLFYSRYSPSDYQQIANLTESSLDTGKVESIDNIKFSTPSFEFTNSHPGTLSIFS
                     QEEIYRQGIDKKPEFSQFIPLSPIGQISTFYAYENP"
     gene            complement(9499..11097)
                     /locus_tag="PKZ29_00045"
     CDS             complement(9499..11097)
                     /locus_tag="PKZ29_00045"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOG37293.1"
                     /translation="MKRDKLLLILIFVFGFILRSIYINKFPPSLNWDEVSHGYNAYSL
                     LKTGRDEWGSPLPLIFRAYGDYKLPLYIYLTIPSVFIFGLNPFSVRLVSLIAGSLIPV
                     LAYLIVKKILPQNQKLPLFAALITLLSPGTIFLSRIALEANLFALLFCLSFYLLISRK
                     YPLSAFVYGLSLFAYNSSRVLLPFFILSLIYFLARHKYHLKKNFYRFLPFLLLTLLTI
                     WQMFNQSGQARYQWVSLLDSGSINRINELRQTYPRFLVNKLTYFTFTAGKNYLKHFNP
                     LYLFFNGGSHYQFNIPQFYLLSSLLIPFLILGLIHLLKNFKNHPEYQLLLFWFLVSPI
                     PSAITRDAPHVLRSILFIPSTIIIIAIGFSRLDFYRPKFSYFCLTFVLVLSQIQFWPK
                     YIQYRQTYASSWQYGYQQAVDYVKSIYSDYDQILVTKKYGEPHEFFLFYWPWNPSVYQ
                     NDSAKIWDYHANWYWVDKFDKFQFINDWEIKDKTITPTLTKNQNTLLITSPENYNQHN
                     SQLLKTIYYPDGSPVFQILKINYD"
     gene            complement(11094..12749)
                     /locus_tag="PKZ29_00050"
     CDS             complement(11094..12749)
                     /locus_tag="PKZ29_00050"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOG37294.1"
                     /translation="MRHKLILVLILVLATFLRFYKLGSLPNSYTPDELAQGYTAYSII
                     QTGQDEWGSQNWLNLRSFGDYKPPLQTLLMIPSIKIFGLTPFAVRLPNAILAVVTIFI
                     TYLIAHNLFHQPSISLLSSLFMAVSPWSLPMSRIALEANLVVFVVSLALLLFITSLKN
                     RQLFLFSISAVLFGLSLFTYHSAKIFTPVLLVILFFYFSLYKHKKYLISLIIIFGSFF
                     SFNFYLNSQIKSDRTGDIAIFNPTDKWQYVSNSQYEIRQNGLQPQLSRVLYNKVTYLY
                     ETFFHHYFSYLSPQFLATNGAGETTYGMIPGYGVLGLIPAIGFVFALIYLIKFPPKSD
                     PSKSLLLLLVIFLISPAIGAIAKGQYSANRVSLMMPFIQIFSAAGLVLFLNQVPKKFS
                     PILSLIFTFTFILTTGTFLARYFYQSNQILAHGMLYGHQQANEYLQNHPADKIIYSRK
                     LSEPQAYVAFFNQINPKIIQSESVNWLDYETSHLMFLDQLGEYKLDKFIFKEINIPSD
                     SQIPNTIIVGRPEEFRDVKPDHIIYYPDHSRQQPAIYIYHTKI"
     gene            complement(12746..14395)
                     /locus_tag="PKZ29_00055"
     CDS             complement(12746..14395)
                     /locus_tag="PKZ29_00055"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOG37295.1"
                     /translation="MTSKPVSWGIIAIIIISFLIRIYRLNNLSLFGDEIDVGYQAFSL
                     SQTGRDYKGNFLPFYLQSLAESRAPLLIYTVVPFVSLFGLNQVGVRLAPVLFGVLSIY
                     LIYKLVLSMSQSKPLSLASAAVLSLMPWHFHYSRAAFEVTLLISLILAGTYFFYLFIN
                     NTKNKFLYLSILFFGLSFYTYNTANIFVPLLVIFLILTNLKTIIHRLKPSLIFTALIL
                     TLVLTFPLVKEIFYGQAAARFSSLSIINNPKVIDQIVVKRTDFSATNPNIEAIFHHKP
                     GKYLTEISKNYIKSFSLDFLFLTGDFLNVRHSIPNFGQIFIIFLPFLLFGLFKMSPKK
                     PLDKLMLFWLIISPIPSSLTLGGGTHATRLFLMVVPLAYFIAFGIIKIPVAKSRLVYS
                     FMIICLSLQIISFAHEYFVHYPKNSFENWNYGYDQIFKNIPSISNHLYISNAKYNSLL
                     PYLFYQKYSPQNFIADKVVDNGPQFNLNSQTTFINQWSSIDHLEDVKAKAKSHDIFVL
                     FQEKDVPGDMDFSQQPLAGFTTIKTIYNPNHTILAQIIQKL"
     gene            complement(14385..15668)
                     /locus_tag="PKZ29_00060"
     CDS             complement(14385..15668)
                     /locus_tag="PKZ29_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37296.1"
                     /translation="MAKTFSKIKNILIFALLFRIILVIISGYHPDILNHLDWGVRFLS
                     LGSKKFYENIFWGVSWPNQPLGSMLLFAFIAVINKFLFQIILFLNNTIPIFPSFIIPV
                     LEQNLHVWLVKLPFILSDIGIGYLIYKIIHQYHPSKAITAATIFLFNPVLIYNSAIWG
                     QTDSLINLLALAGLYLIYQKKYFSGVILFLSGFLFKLSLIIYLPILGLLFLKRLKDWK
                     KFIAPVISFAVLIFVLAIPFAFGDKNPFEWLWYMYTNRVLVRQGSMLNGNAFNFWAGL
                     FGINLSLSEFTKFWGLTYQWWGRLLYLLFLLPVWFKFIKSKLTLTNLFYALMLSAFGS
                     FIFLTNMHERYLYPVFPLLTTIIFLPKSKFNLKHLLALSLIHFLNLYNLWFYPLVSPL
                     KTLLIGFNFLTCRLLSFILLVTCLVYLVKYLKSDL"
     gene            complement(15644..16447)
                     /locus_tag="PKZ29_00065"
     CDS             complement(15644..16447)
                     /locus_tag="PKZ29_00065"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOG37297.1"
                     /translation="MKPLISIIIPTWNTARVTLKCVKTIQKHLKNFPYEIIIIDNGSI
                     DNTVKLLTPTSVRLIKNKQNLGFATANNQAAKIAKSDYFLFLNSDMELIDQSLIQMFA
                     YLQNNKNIGCIGPKFLNPDMTSQASVFPSQTIANAFKEFWLGQKTYSKYTPKTKSPCP
                     VEYISGGALLISKSIFNQIGGWNEKYFFYYEDLDLCRAVHRLNRQVYYYPKCRIIHRH
                     GFSGQSLADAKNQWRRLIPSSIKFHGYFKHYLINFIIWSGQKWQKLFQK"
     gene            complement(16431..17753)
                     /locus_tag="PKZ29_00070"
     CDS             complement(16431..17753)
                     /locus_tag="PKZ29_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37298.1"
                     /translation="MKKNILILIFFSLSFISRIYISNLSTPKGDILVHQEWSKVLYHQ
                     GLTGSYFFNNWTYTPPTQPSLMMMAYSASRSIYENRNIFSAMHNLIKIPPAFILLGFQ
                     QYGQILTLKIWEFLATVIIAFIFYFYYSSKKSPNIGLIIFCLILFHPILFFTNSVWGQ
                     NDLLATIFIYIAFLILFTKQKIFSPVVFILGILFKPTIVVILPFFGILYLYLNTKKGN
                     TLNKIKNIVIPLVLSLAIFYLSFLPFIPKNHNHLQYIRSIFDSRISNSSKGVELASVS
                     AFNLYSLVFTIDKTYSAITPHFKLTYISWSFFVLLNIFFISKFFRSKKINFNHSLFTI
                     FFISQGAFLLMTGMLERYFFPTFLSSLIIMALYWQKFGKLMLIQQLIWFLNLIYAYYH
                     RDIEFISHFFKNNDFIFIRLLSLSSLFIFFLIAKKYLALLNSKNETLN"
     gene            complement(17750..19210)
                     /locus_tag="PKZ29_00075"
     CDS             complement(17750..19210)
                     /locus_tag="PKZ29_00075"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOG37299.1"
                     /translation="MFTILSLLSYKFFFNKWIGYEAIPNTFIFDEHDYPFVGYSFRKT
                     GVPTGWSTMEIYKELDDKKDNSNLSFDGISINTQGQKPNLKNKQLFNYPVTTVIDVDI
                     GKGKETIRIVQPFFDHPIFGSWLYSLPIKNIEKFDDIKPSDYRKVSLIISVITGFSIF
                     LFSFLLFKNYFISFLSFFLYSNVPEFVLMSRFALFENILIPLSLITFSLIILFNKYKS
                     KYIFLLLAGLFAGLSFITKESGIFIFLTGLIFLVKEKINKKTFLSYIIPFVLISISYY
                     IYMYYLSPDLFFKLLFSQAHREWFGSLSFFHQIIQPNFSGFPKSGYWLFGIISIFILS
                     IKNYKKYIVLLTPFIIYLFIFLFLGGLNYPWYYLPFLPFIIIASSVLIYNLVILPNLG
                     NFTLFFLLVFSSSFFWGYSSFHDSQKSFLIYRILLLFFTLIFFIRKKKVTKFYYYIWL
                     TFVLMIFYQVNKWNQQSLLYMISNWGNFSPNLSFPL"
     gene            complement(19240..20706)
                     /locus_tag="PKZ29_00080"
     CDS             complement(19240..20706)
                     /locus_tag="PKZ29_00080"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOG37300.1"
                     /translation="MKKFYQLLLLIVIKIKTFVDMLKNSIKVIILICIAILSLFLRKR
                     DYAQIPIPGQSTDEYSNSWVGLSLIETGMPIGISGLPNYQNETKEYVNVDRFTQYLSS
                     DPLTLNYPWMDHPPLLGLITGGYAYTQGARVFEDTIALFIRRPIILISTISVALIMIY
                     CWLNFGFLSAIVSGLIYGTTPLIVLSGRMVQSENAIIPMFLLTMIFISQYIKSKKDHW
                     LILAALASGVATLFKLTGFVCYLFVFLSLLNYYHNFTKQFFKDFFFYLLIALPVTSLF
                     LIYGSVYGLKHFFNIFLSNSHRFYGIGPSAIFDLIRQQRLTQHKFLPEVWIVVSWIVS
                     FTYFLKKKIKYKIIVPFVALISYLIIYVFFGSQPYGWYAFPFWPILIIVFSHFFITSL
                     KKCRQFYLLIINLIIVLGVNISRIVGIFEFQPYSKYWRFGVSFILFIGIIYFLINKIK
                     INKNLKTAISLILFGFSIYLNLIYLNKINIDFWWQNIS"
     gene            complement(20633..21109)
                     /locus_tag="PKZ29_00085"
     CDS             complement(20633..21109)
                     /locus_tag="PKZ29_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37301.1"
                     /translation="MNRQKFLLVYLSIFTLIGFLIFFGLRTHKNKNFSPEKLSYLTDT
                     VSKFRDKTDDLCKYKILGDPQVGDQYVKINFWCTDSRSSRSTLALIAIPNPTIDGIFS
                     EYSRIINFDKDLISQQKWVCFYNDKEISNKDIFEKILPTSTINCYQDKNLRGYVEK"
     gene            complement(21114..21989)
                     /locus_tag="PKZ29_00090"
     CDS             complement(21114..21989)
                     /locus_tag="PKZ29_00090"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOG37302.1"
                     /translation="MKISVIVTNWNGLSLLKKNLEIVIKNSPEAFEVIFADDASEDDS
                     LKFVKSLQKKYRQLKIISHQNNLGFGANSNYAVSQAKGDLVVLLNNDIIPQKNYISHS
                     LKHFSDPKVFGVGFCETNNKNWARFFWQGGYLQHQPGTTGLNKTHISGWVSGGSSIVK
                     KNLFQKLGGFDPIYHPFYSEDLDLGYRAWKSGYTLLWEPKSQVEHHHESTMSKFSPNL
                     LNYVKERNRLLSTWRNITDPKLLFQNKLALVGRVLTGPNYVKIIRAAKKQVQKHPAPI
                     VFPKLTDLELLDIFK"
     gene            complement(21991..23175)
                     /locus_tag="PKZ29_00095"
     CDS             complement(21991..23175)
                     /locus_tag="PKZ29_00095"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOG37303.1"
                     /translation="MATKKAAIYDPYLDTLGGGERYCLTVAEILIKNGYSVDIFWSGD
                     KTLIEKAEKRFSLDLKELKMVPDIFGIKPQNLNLLETHDDLSKFTNRVADPFRLKVKL
                     SSISKKIKNNRKYDVLFYLSDGSIPLLFGKKNYLHVQVPFVNHYDLPSRIFRRLKSKL
                     FTNIICNSQFTAKYQDQDVKNKVLVVYPPVDVDKFYASKSKENIILSVGRFDNILNAK
                     KQDVLVDAFRQLSQKSPIGNWKLVLAGGSLTDPEKNSFLQYLQKKAANLSVEFMVNPP
                     FNVLQELYSVAKIYWHAAGFEVDETVHPENTEHFGITVVEGMASGLVPVVVEQGGIPE
                     IVDNGVSGFLWKTVDELVAKTQKLIDDPEILKLMSQQSLINCQQFSKSNFEKKLMGII
                     GK"
     gene            complement(23191..23985)
                     /locus_tag="PKZ29_00100"
     CDS             complement(23191..23985)
                     /locus_tag="PKZ29_00100"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOG37304.1"
                     /translation="MAITKPPISIIILAGNEQTMILDCLKTCNWVQEIVLVAANSSDQ
                     TVPLVKKHFPQVKIVRTEDEYNKNFSKWRNLGFKSSTQPWIFYVDADERVPANLIKKI
                     TQIVSSSPPKFTHFAISRANHFLGQRVFYGDSYPDYVKRLFNREFFTGYKGVLHEQPI
                     ITGELGYINNDLLHYTHRDLTSMVNKTLAWTDTEAKALFKADHPPVVWWRFIRMMLTK
                     AWERLIKQQMWRDGQVGWISVIFEVFNTFLIYARLWELQQKQNQKI"
     gene            complement(23976..24893)
                     /locus_tag="PKZ29_00105"
     CDS             complement(23976..24893)
                     /locus_tag="PKZ29_00105"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOG37305.1"
                     /translation="MYKTAILVLNWNQPDLTVNSVESILKINFRQIDYQIILIDNGSS
                     DNSLTIFRDRYHSHPKVKIITTNSNLGFAGGNNFGIDYALKNNFDYVLIINNDVLVDP
                     DFLINLLRHTKDYDILGPKIYFAPGFEFHRQRYTKKDRGRVIWSAGGQIDWRNIYGSH
                     IGVDEVDSGQFDDINNKVDFLTGCCLLVKSSVFHQIGLLDQKYFMYLEDADFCRRARL
                     AGFNLAYIPSSKIWHVNAGSSGSGGDLHNYFLSRNRLLFGFRYASFRTKIALIKESIA
                     KLLDPKVAKWQKIGITDFYLKKFSKGSWQ"
     gene            complement(24897..25832)
                     /locus_tag="PKZ29_00110"
     CDS             complement(24897..25832)
                     /locus_tag="PKZ29_00110"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOG37306.1"
                     /translation="MKPKINVSAVINVRNEAQHLSKCLKSIKDFVDEIIVVDMHSTDD
                     SVAIAKSYNAKVFSYRPMKYVEPARNFALSKASGKWILLLDPDEYLSKTLKRELKKIT
                     LRADVDFVKIPRANIIFGKWIRHANSWPDYLIRFFKKGAVTWQKEIHSQPVTKGNGLT
                     LLDSEKLAIKHNNYQNLNQFVLRAMRYSSIQAEELNAKDYKVKISDFLLKPVQEFNSR
                     FFFGEGFKDGLHGLVFCLLQAFAICLIYMKLWEIQGSQDKPLPKESFISASQESVFEY
                     DHWFTRFFKNEYKPGIFKTGLIKLRHRLNRLTKNF"
     gene            complement(25849..27522)
                     /locus_tag="PKZ29_00115"
     CDS             complement(25849..27522)
                     /locus_tag="PKZ29_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37307.1"
                     /translation="MFKTKKSVIATILISSLILSLTMVRSGLKYDFGLGFWGPNGHDG
                     IWHLSLINQLKQQIPPPNPVFSGTTLSQYHWGFDLLVALISKFTFLNPSILYFQILPF
                     IFALLIGFLSYKLAYLITKNNRVSLFFVILNYFAGSFGWIYTLLVNRHFGGESLFWSM
                     QSASTLLNPPFALSLILTLTGLILWQKYQTKSPKYAVIIGLIFGFLSGIKVYAGILVG
                     LGFSLLCLYNLFFDKPKFKFNAVICLTMAFVSLIILALLGVLNSSSLIEFKPFWFTHS
                     MIESFDKFYWPRLASYRYNQTNKLISAVIELGLIFIFLIGNLGLRFLGIFSLNHQHSK
                     TQSSFKFLTLSICLLAFAIPLFFVQKGTAWNTIQFFYYGLFFANFYFAIFLSHLWQKY
                     RLTSLIILFIACLTSFATIKDYLGFPPPASLPSSEIQALNFLKKQPQGIVLTYPYDPH
                     LKTDMSTPIPLYAYETTAYVSAFSGHQSFLEDEMNLDITGFDWQTRRHEVDKFFTTTD
                     EFYARGFLLNHKIDYIYLTNQQKLPLTIEDLQIDQIYDQGQIRLYQVRK"
     gene            complement(27498..28955)
                     /locus_tag="PKZ29_00120"
     CDS             complement(27498..28955)
                     /locus_tag="PKZ29_00120"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOG37308.1"
                     /translation="MAKINLQEIKQKTTKNVLFLSLRNIGIQSISTVGFFLLTILLGT
                     AEVGLFAIVAESVGILGYFSDVGLAAALIQKKSEVKKKELQSTFTIQQILVFIALIVI
                     FFVYSKISLTKGYGPKEFWIFTSLCFSFAMASLKTIPSVLLERKLNFKLISTIDIVEN
                     VSFYLVAVVFAYFGFGAYSYAIATFVRSLLGLVLIYRYSRWPIGFALDWASIKELFKY
                     GIPFQLNSFIAMAKDRLSSLFVAGIIGREAFGILSWAQKGPRIPLSFMDAIMKVTFPT
                     FARLQDHKDILKKSLERSLYFIALFVFPMVAGITLISPDIINIIPKYTKWAPAIFPLY
                     FYAANVAIAAITTPITNAFNAIGKITLTTKFMIMWTVLTWILFPWLSIKFGYLGTAYA
                     TLLVGSSSFIVWYFAQKIFGVRVLSAISKPLLATILMLIFSLIFQNFGFYGWVSLIGK
                     VVISVSVYLLFHLLFSKNEIRWFYQQLLCLRQKNQ"
     gene            complement(28928..30676)
                     /locus_tag="PKZ29_00125"
     CDS             complement(28928..30676)
                     /locus_tag="PKZ29_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37309.1"
                     /translation="MSKLIKHKYFWPILTFLFTIPAFFFLLKPGYYNMHDDMQMIRQL
                     EMEKCFQDGQIPCRWTPDLGYGYGYPLFNFYPPMPYLVGQVYRTFGFSFVNSVKATAV
                     TQIILAAAFMYLLSASIFGPAGGFIASIFYTYAPYHALNIFVRGAMNEAWAAVFFPLT
                     FYYARKLVLEQKNKFIFGLGASFAGILLSHNPMALTFTPILFFWVIFWIFSRYKKPFF
                     PFIKNQFSLIIKLILSGLFAAGLTAFYTIPVLLETKYVQINTMFEGYYHFSVHFAGLY
                     QLFISNFWGDGASVWGPNDDMSFMVGYLHWIIPLILVIFSLIFIYKSRSKISKLDSRF
                     WLILLLVFMAFFTIFLTHNKSTFIWLLFPIIQKIQFPWRFLNHSSFLLSLSIGGLTLL
                     INKYFPKKIIVISLALSLAVLLLNLGYFTPIQHGPITDEQKFSGKAWVNQVTSGIYDY
                     LPTTARIAALGPAKEFIDDITPKDVFYTITGGKKGSDWMLFNLNLNQSSRVILSQLAF
                     PKFVVLDNGKPIATDIEPELGRLVINLDAGYHQIFVKFTNTPVRTVSNYISLVTWLFF
                     GLFLSHPLWRKSISKK"
     gene            complement(30697..32223)
                     /locus_tag="PKZ29_00130"
     CDS             complement(30697..32223)
                     /locus_tag="PKZ29_00130"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOG37310.1"
                     /translation="MIKKNSFWFKVILFLAVAGVYFFTRLQHLDAIPVFGDEAIYIRW
                     SQIIKNEETLRFVPQTDGKQPLFMWLTSLSLRLVADPLIAGRLVSVVAGFFTLIGIFL
                     VASIYPSFFEQSFNPANFIKNSLKNNFYLGLLSAIIYIFLPFSFFFDRLAVPDNLLSA
                     FGVWSLFFSLLLAKFRRLDLSLILGMVLGLSWLTKSPAIYFVALSFGTFFILNLDNLK
                     STYLPLISAILAFLIYNILRLGPQFHMIALRNRDYIWPLSEIIKHPFDPFRSHFGSAI
                     TIFGQYISWPILFFCLIGLGLYFFYQKNTQLLAILAWAFLPLLANSAMARVFTARYIL
                     FILPPLVVLITLGLSVWFSRNFPKIIFILLLLIPNIIWLKNISLTPFEVKLPSTEVGY
                     LSDWTSGWGIKPAADLLKNRAKEANVIVGTEGSFGTLPDGLQIYTDGVPQLTVIGQGL
                     GFTQIPSSLTTARQFGDEVYLLINQSRLTLSSLDQDKLALVQSFAKPDGDKLLLYRLK
                     "
     gene            complement(32207..32974)
                     /locus_tag="PKZ29_00135"
     CDS             complement(32207..32974)
                     /locus_tag="PKZ29_00135"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOG37311.1"
                     /translation="MTKISIVIPCYNEENNLKRGVLDEVNQFLTKQKFKWEVLVCNDE
                     STDNSLKLVKKFVANHPGFRVLDLPHGGKPSAVWGGIKEAKYPIVLFTDMDQSTPLKE
                     SLKLLPYFDKGYNVVIGSRGAVRQGASFVRKLMAKGFLFGRRLFLLRDIIDTQCGFKA
                     LKTDLAKKIFPELQFFKEKSNNSGWRVSAFDVEMLFMAQKWGYKIKEVLVQWQDEDAS
                     TTKGDDATRFKKESKQMLQEIIRVKRNDLLGLYDQKK"
     gene            complement(32989..34470)
                     /locus_tag="PKZ29_00140"
     CDS             complement(32989..34470)
                     /locus_tag="PKZ29_00140"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOG37312.1"
                     /translation="MIKKFLTQNWLLVLILILGTFLRFYRLGDYMEFLGDQGRDVLVI
                     RNFFKHKDLFFIGPQTSIGNMYLGPYFYYLIAPSLLLANFNPVGPAAFIALLSLATIV
                     LVYFVGQKWFSRPVGLIAAFLFAISPVVIKYSNFIWNPNVMPLFSLLFIYFFFEAIIQ
                     QKYKYFIYASLSFVMVINSHYLGLALLPFPGLYWLFYLYRHLKTKSKQLKPFLTNSLI
                     AILVFILSLTPQVLFDLKHNGQNIKALINFFTYRETTVSIKPYKAIPELPAIFNQVNT
                     DLLAAQNQTFGIIISLALAVLIGFYLLSKNTKKTYFWVIFGWYLFGLSALALYKQHIY
                     AHYFAFLFPAVFILIAILIHLFKYIGIPLFILITIISLINNPFKNQPQNQLVHAQNIA
                     TSINNHLDPSDGQYNVVLLASYNDFKALAPRYFLKSDNLLSPEKYQEADTLFIVIDDP
                     NKWPDGINSDIWEIKVFESQNKVDEFITSDGVKVVKLQKIKNN"
     gene            complement(34463..35191)
                     /locus_tag="PKZ29_00145"
     CDS             complement(34463..35191)
                     /locus_tag="PKZ29_00145"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOG37313.1"
                     /translation="MKPYLIDELSVFYPCYNEEKNIKSTVAKTIPVLKKITGKWEIIL
                     VNDGSKDNTAAVLTSIKKQFPQQIKIITHNPNRGYGGAVKSGLYNSKYKWITFTDSDG
                     QFDFGEVVNLIKKQQQTQADIVIGYYLSRQVSKLTILTSKIWELIVFILFGLKVTDID
                     CAFKLIRKKVVDTIPKLESERGAFISSEFLIKAKKAGFKIAEVGVHHYPRTEGQATGR
                     NLKVILKSFSDLFRLWYKINFKRD"
     gene            35309..35953
                     /locus_tag="PKZ29_00150"
     CDS             35309..35953
                     /locus_tag="PKZ29_00150"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37314.1"
                     /translation="MKQKNLLLALAMAAIMAGSLVSPALVKADSYTSVDNRKTLSIDK
                     KIRYIFSANYVDNISSSTKVFTQGDIVEFKIKVTNTGTTNMRNIKVTDQLPPFLKLIF
                     FPGSYNKTDNKVEWTIDELNAGHSQEFLIRAKIDQATEVKTLTKETNVAIAKVDEISE
                     KDDAVYYIGSPSAQTKGGQIIVPETGNVGLIFQTGGVISLGLAGLALRKKIRGY"
     gene            complement(36007..37191)
                     /locus_tag="PKZ29_00155"
     CDS             complement(36007..37191)
                     /locus_tag="PKZ29_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37315.1"
                     /translation="MFKKFFILVVGFIFFASLTQTCRAQSEEELKSQIDQYIQKLDEL
                     GKSKDTLSNQIKLLDSQHQLTLLRITQTENSIKKLETEISQLVVEIDKLEIDITKLSE
                     SYIHQTVQNYKLQKRIPVFAYLFYSNLNTFLEQHRYISSVQKSSQDSLISMETIRSNY
                     DNQKTAKAKKQAEMENLQKTLSSQKSSLISQKAAKNSLLKTTQNDEKKYQQLLAAAQS
                     QLAAFKNFSSSAGGSTCLGSSPGAGSDGNFYSQRDPRWCRQFIGNSRDTIGEVGCFIN
                     SVSMVYKKLGFDITPSAYAANSSNFWGNTAYMVAPSPPSGYTYKQVSYNSGVIDDELK
                     AGRYVIAQVKMATVAGMHFIVIINGSNGNYTIHDPWFGADQKFSDRYSPALVMSLRLI
                     TK"
     gene            complement(37194..38066)
                     /locus_tag="PKZ29_00160"
     CDS             complement(37194..38066)
                     /locus_tag="PKZ29_00160"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HOG37316.1"
                     /translation="MTPTKTAWHHIRRSPIQSLTALFLMTFSFILISAFVVVNSGLSQ
                     ILNYFETKPEITIFIKDGVDQISIDNLKKEITNFPNIKEVKFISKDKALEIYKQQNKD
                     NPMLTEMVTASILPASIEVTVSDPKTLDQIYQNYATKTDIVDEIIYQKDVIESLLDWT
                     NAIRRGGLVITIVIIAISFISIVSIIGMKTTNRKDEIKISRLLGASKFYVKKPFLLEG
                     IFYGLLGSFIGSILVFITLYFSRNSVNSFFQPVNFVSGDLSFFSLIIFIELIFGCLIG
                     LLASWIGVKRYIKF"
     gene            complement(38053..38784)
                     /locus_tag="PKZ29_00165"
     CDS             complement(38053..38784)
                     /locus_tag="PKZ29_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG37317.1"
                     /translation="MKIVFDSVDKNFGLITALSNISFTVDQGEFVFIVGHSGAGKSTI
                     IKLLLNQIKPSRGQITVDGTDLSLGTKNEIDLIRRQIGVIFQDYQLIMDKSIEENIAL
                     SLDIIKYPRSKISTKIDEVIKLVDLNSRRYLFPAQLSGGELQRAALARALAIEPKIIL
                     ADEPTGNLDVENSWNLVKLLKNINEKNKTTIIMTTHNQEIVDSLDRRRIEIKDGQIVK
                     DFTPGTTPKAAPVKKNTSSTKNDTH"
     gene            complement(38786..39265)
                     /locus_tag="PKZ29_00170"
     CDS             complement(38786..39265)
                     /locus_tag="PKZ29_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37318.1"
                     /translation="MDIPNLSPNKPVSPPPPTVSTTPKYVYAIIVVLAIVAGFWISRF
                     WPSQTTSTVDADTSLVDNQAAKIVEGEEIKVGVVYGNLSKTFADSAVGVIKSGGVNGE
                     GTHTLERPGGVTQNAALTSSVVDLDLFVGKQVEVKGETNKSNKAAWLMDVGSIKITE"
     gene            complement(39302..40324)
                     /locus_tag="PKZ29_00175"
     CDS             complement(39302..40324)
                     /locus_tag="PKZ29_00175"
                     /inference="COORDINATES: protein motif:HMM:NF015755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HOG37319.1"
                     /translation="MSKIIRYTLLVLVCFFLLFAGLVFWGFKKISYDLGQSPQYLLDL
                     VTTARLNYDRHQSQKTNFIVLGLDKRDDQLEKTQTTDTIIFVSLDYSRQKISLVSLPR
                     DLWFYDLNTKINQIYPLSLNEPDSFEFIKAKFKQLTGQSIDHVLVLTTDNLIDFVDLI
                     GGVDLVLDQGFVDDQYPNPEYINNPSPKIPIYKTVEFKSGPIHLDKTNVTEFVRSRKG
                     GQTAAQGGTDLARIHRQQLLIEAILDKIKSGNFIQNYSQAVKLYHFWNRSLIKDISDN
                     DIVAIISHLDYQIKNLTFDKINIPIGNTAADGLIYHPNRLFNRQWVFLPSDKDYTSFR
                     HFFDAI"
     gene            complement(40325..41398)
                     /gene="prfB"
                     /locus_tag="PKZ29_00180"
     CDS             complement(40325..41398)
                     /gene="prfB"
                     /locus_tag="PKZ29_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_161931791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOG37320.1"
                     /translation="MNILPELKTKFDSIKQKLDFVQNLERLTELEKKSQQANLWDNPA
                     SAQELLQSISDLQKTISQINSVASQIKTIEEFSDLLTQTPDPSLESEVDHLIKQLETD
                     ISALELQTYLSGKYDQKSAILSIHSGQGGTEAMDWASILQRMYLRFFERRQWKYEVLD
                     LIPGEEAGIKSVYFKISHPFAYGYLKKESGVHRLVRLSPFNADNLRQTSFAKVEVTPV
                     LDFASDFKIPPEEIEFSAFRSGGHGGQNVNKVSTAVRLVHQPTGISVSCQSQRSQEQN
                     REMALQMLTSKLWAIEEEKRTAEQKNIKGDNVVAGWGHQIRSYVLHPYKMVKDLRTRH
                     ETQDTAAVLDGDLDAFIEAELKL"
     gene            complement(41402..41785)
                     /locus_tag="PKZ29_00185"
     CDS             complement(41402..41785)
                     /locus_tag="PKZ29_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37321.1"
                     /translation="MSLKKKEKTKIGFSSILRLIIFLFLVFILIGFLAGKTINSKPTD
                     NPSAVLGESFGGDILGEFYSQLPESSRSQIENINQTPIGKFFADTIKNIQIQLDGFPQ
                     KQIKDIKKNLIKNISDDMIRNIDEN"
     gene            complement(41789..42202)
                     /locus_tag="PKZ29_00190"
     CDS             complement(41789..42202)
                     /locus_tag="PKZ29_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37322.1"
                     /translation="MNKLIYIFIGLAVLVGLFFLLSQPPKNNALNIANFEVPADFNNY
                     DLVFYWGDGCPHCKNVDKWLTDNNKDNQLKIYSKEVYQDKNNQQELLGLASKFCPELI
                     DNGGIGVPTSFDPVGQKCIQGDTPIIEFLSAKLKP"
     gene            complement(42205..42795)
                     /locus_tag="PKZ29_00195"
     CDS             complement(42205..42795)
                     /locus_tag="PKZ29_00195"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOG37323.1"
                     /translation="MKQNLKLFILVVVSSLLISGCTSKKPLPTNMELNNLPTGANVTV
                     SPAATPQSQPVVSLKTKNGEILIKLYQDKCPQTVANFLKKADSGFYDGLTFHRVIKDF
                     MAQGGDPDGTGMGGGKQNSELNDAPFVRGSLGLARTAETKLISNDSQFFICFTTAGCQ
                     HLTSEYVNFGEVVSGLDILDQINQGDKIISLTSKTK"
     gene            complement(42801..43562)
                     /locus_tag="PKZ29_00200"
     CDS             complement(42801..43562)
                     /locus_tag="PKZ29_00200"
                     /inference="COORDINATES: protein motif:HMM:NF024919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HOG37324.1"
                     /translation="MSNKFWYFLIFISLPVLIYFSLKIAYPQKIDQKIDTVVVKINDL
                     KETVAPVPTIILDSGLPNKHLIKTAFIPQSPEKNWDQPWQDACEEAALLTVNYFYKNI
                     TPASLDIKNDILAMIKYEADQNFTHDVDLSQMVLIAQNHLNYAAKIIDNPSLEDLKSY
                     IAKDIPIIVPANGKTLYAENKHFKSGGPYYHNLVILGYDDNTSKFTVHDVGTQFGAYF
                     KYSYNLLLKSIHDFPTSGVKQDINQGSPRVLILLK"
     gene            43649..45490
                     /locus_tag="PKZ29_00205"
     CDS             43649..45490
                     /locus_tag="PKZ29_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006193030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOG37325.1"
                     /translation="MPKLTDIRNIAVIAHVDHGKTTLVDAMLKQSHMFRSNQDEMKMD
                     RIMDSNDLEREKGITILSKNTSIFFGETKINIIDTPGHADFGGEVERILNMADGALLI
                     VDAAEGPLSQTKFVLKKALEYGLKIILVINKIDRKDQRIAEVITDTENLFLSLVDDLE
                     MLDFPIIYSIGKDGKAYSQLPANFEQKADINPLLEQIIKTVPNAVQEIDKPFQMLISA
                     FEKNEYLGKLSVGRIHTGVIKKGMQVSLVDPDQKTRGNFRIERMYVNEGIKKVEVDEA
                     DSGEIVYLAGVSNIEIGQTLTDVNHQVALPTIEITPPTIKASFGPNTGVFAKDEAKFL
                     TSRELKERLKQEVETNLGLKLEDDPVDSIRMLVSGRGELHLAILIEELRRQGYAMEIG
                     KPEVILKTIDGVVCEPFSELTILTPEDFVGTITTEMGKRKAEMTDMQTDDKIGVKMVY
                     KISEKNALGLRSILMTETKGMVSMSSLFLGYEPKRDEEKKERNGVLIASESGKALSYG
                     LDLAQKRGITFVEPTEMVYEGQIVGLRPLMGDLEINVCKGKQLSNMRAAGSDDSIILA
                     PATKYSLEECLDFINSDELIDVTPKKIRLRKKLLSKIDRVRAERNTR"
     gene            complement(45493..45813)
                     /locus_tag="PKZ29_00210"
     CDS             complement(45493..45813)
                     /locus_tag="PKZ29_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37326.1"
                     /translation="MTDITSTPPTPNQIDKNQELIDEIKKLTYQIKLQNEPWRRIGRS
                     FTNGFFASLGSLLGTLILVSILSYFLLQQPFFKNLVGFFQNPPKSNNSLELFLPSLFP
                     KPSP"
     gene            complement(45820..46215)
                     /locus_tag="PKZ29_00215"
     CDS             complement(45820..46215)
                     /locus_tag="PKZ29_00215"
                     /inference="COORDINATES: protein motif:HMM:NF023395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HOG37327.1"
                     /translation="MDDCIFCRIASGSLPCHQIYEDKDFLAFLDINPVAVGHTLVIPK
                     HHYRWVYDVPNFDQYWQVAQKIALNIKNSSLKPEFISFLTMGNDVPHAHIHIIPRNLG
                     DQIQPVLQPFPHQKLDDKQLVEIKNKIKL"
     gene            complement(46218..47240)
                     /locus_tag="PKZ29_00220"
     CDS             complement(46218..47240)
                     /locus_tag="PKZ29_00220"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOG37328.1"
                     /translation="MLSFKFTSKKIWREIDRSQKILLHLHPGSDGDSVGSALAFYHVL
                     KNLGKDVTLIQGDNNLPLNLSTIPGADKILPQNIFQIDLNQFDLFIILDSSSLRQITR
                     LGDFKFPRTLKTIVIDHHPSNEKFAKINLVVPHSPATAQVLFDLFSVRKIKITPKIAA
                     CLYAGIYTDTGGFKYAGTTHKTLVIASQLAKIYPKFSQLIFDIENNDPPERLKLISLL
                     LGSIEIYYSGHVAIASLSYEDIKKHQLESVVNTSYSDVANMIKSVIGWDIALVFVEIQ
                     PQTVKVSFRTRDSRTYDLSQIAVATKAGGGHKAAAGATINQPLSEAKTLVLQIIKKLH
                     PKIDKI"
     gene            complement(47243..48148)
                     /locus_tag="PKZ29_00225"
     CDS             complement(47243..48148)
                     /locus_tag="PKZ29_00225"
                     /inference="COORDINATES: protein motif:HMM:NF019155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HOG37329.1"
                     /translation="MGRFNMPEDTPLTHPLVSRVIEQIQVKVEGFHFDARKNLVEYDD
                     VINKQRQIIYRLRDEVLLNQKETPDKNHQLILKYLTNQVTGIVNLNTDYDSHRLDLEK
                     TSLEFNEILPLNASGRSQLKKDLAKSDNPTEFLTSILEDQWQSRVKMFGKEIADSILA
                     FSLISTLDPLWVEHLTALDDLRDGVRLRGYAQKDPLVEYRKEGYEMFQRLLNQFEYNL
                     ARKIFRLEPVAPQATSPQNVTEGRGDSLTDNPQAPSPDQSLPDADSLSPLPQPIKKDK
                     TVGRNDPCPCGSGKKYKKCCYPKFG"
     gene            complement(48238..48378)
                     /locus_tag="PKZ29_00230"
                     /pseudo
     CDS             complement(48238..48378)
                     /locus_tag="PKZ29_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315501.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GHMP kinase"
     gene            48361..>48579
                     /locus_tag="PKZ29_00235"
     CDS             48361..>48579
                     /locus_tag="PKZ29_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37330.1"
                     /translation="MSHGIYCTWGPAREALLAANIPVITYGEGKKRDTEKFNWNTSAD
                     WWDVSAEWDKVRDVPLTPAQQKTIDDYLA"
BASE COUNT        16769 a         7400 c        10728 g        13682 t
ORIGIN      
        1 cgattttttt atttaaaaaa tatttataaa attggttatg aataataata ttttttgata
       61 acaaccaatt tataaatttt ttagtcgggt agttatataa aataaaattt aataatctgt
      121 tatctattgt tgccattgtt gtctgtttaa ttgttttaaa agtccaagtc gatatttgat
      181 agtgttaata gtataaaggt agagggtaat aaaacaagca agtggatgga aaaaccaggc
      241 aacatcgggt ttgtttaaaa aacctcgaag gctgtcgaaa agtgaaggaa aaactaagac
      301 cgtataaaga acaaacttta ttgttccaaa tttgtttgtt tgatcccaat tgtattgacg
      361 aagtttttga taataatagt agtcagtaat acgacgattt tgtttacgga taaatttttt
      421 tacagatgat tcacaaaaag tatgaataat cgcctgtttt actttggcaa aataaagtgg
      481 ttttttattt ttgtttataa cttgtgaaat aatatcgatg tcaaaaaggt aatctgactt
      541 gaggttattt tttaaaaatt cggttctaaa aatagtacca ttggctccaa tcgttgggat
      601 aggttgacgt ggttttaatt taactaatat atatttgtct ttatcgattt gttctatatt
      661 taggcgtgtc cattgattat ctatataatt ttttctatca gagacaccag taacaaaggc
      721 gtatggatcg ttggctccaa taagagatga atatctttcg ataaaaccac cggcaggtct
      781 ataagtaaac tcaattggtt ctgaaccaat tatttttttg tccttttcta aagggaacaa
      841 catttggttg agccaattat tgtcaggaag aatattgtca gaatcaatca gacaaatata
      901 tttcccagaa gcttttttaa taccaattgc tttaccggct tcggcggttt ttagggaatt
      961 tttgacaatg attgaattgt attttttggc aatattaata gtattgtcgg tcgatccacc
     1021 atcagaaata actatttcat aatcactcga cggaattgat tgttttttga ttgaatctag
     1081 acatttttcc aaaaccaaac ttgcattgag cgtgggaata ataaaagaaa tttttttcat
     1141 gaaaaatctt ttaatttttt aaaatattta aaagcataat tggaaaggtg cttaatatcg
     1201 gcaaaagatc tgatagaaat aatttttttg aaaataaatt tgggagtgat aacaccctta
     1261 aataaatttt ggactaattc gatttgtttt tttgggggaa ttggtgattt gataataggg
     1321 gcgcgcatat cataatcatc ccaatttgtg gttaaaagta aattattttt ttggcaatac
     1381 tgaaataaag gagttcctgg ataagggatt agaatagtgg cttgaagact gtcggcaaga
     1441 ttgttttgaa aaatataacg agctagatta agagtcttgg tggcctgttt tagggtttct
     1501 ttgggatatc caatcatgac agtaacatgt ggttctaatt tagctttttt ggccataatt
     1561 agggttttta aagctgatga ggttttctca tttttattga taaaatctaa tgttttttga
     1621 ttagctgatt caagaccgta aagaataaac ctaaatccag cttttttcat taaattgtat
     1681 tctttttgac ttaggacacc aaagcgcata ttacaaccta agactacttt tttgtttaac
     1741 cctcggttaa taagctggct acacaaatca tttagccact taccaacagg taaggtaccg
     1801 gaatcatcaa aaatttcttt aacgccaaaa ttatttacca aattttcaat ttctttgata
     1861 gtatgatcaa ctgaaaaaga gtgaaaattt ttacccggga aaagagtcgt ccaactgcaa
     1921 aaagtgcacc tcccccacca acaatcccga ccagacataa tgtagctacc aggtttatat
     1981 ttataattgg tgttgttgtt ggcatagagc tgccagtgag taagtttcct atcaatgatt
     2041 ggtaagatat ttaaattgat tggcttttga agtttataaa ttttattttt tggagcgtgt
     2101 ccactattga taagttcaat tagttcatta agtaaaaaat cataatggcc accaattaat
     2161 acataatcaa ctggacaatt atcaattgat tcttggggaa gagcggttac atgatcgccc
     2221 attaatacaa tttttaattt gtaattttta atttttaaac tttcttgttt gatttgatta
     2281 ataatttccc aatgtttttt gattactgga gtcttagtct ctataacaac taaatctggt
     2341 ttgttcttga ctaattgatc aatccattgt tgataggtta acttttgagc tatagcatca
     2401 tcccaataaa tttttagccc tttgctttga agtaaagtag cggcataagc cggaataata
     2461 ggataaatta cattgcctgt atttgtccat tgaaattgtc ggttttgaga taaaaaagca
     2521 gttcctttgg gtgactcgag tggagggtaa gaaaaagaaa cagatttaac caccaaggta
     2581 tttaccggtt tttttgtcga cggcgtgtgg tttaaatttt gattcttttt tggataaacc
     2641 atataggaat agtataccgt aataaaagtg agtcaaggga atggcggtag cggcaagaaa
     2701 ggcggtagta gggcggtttt tgtcaattat aaatttaacg aaggtggcaa ataatattga
     2761 taaatacaaa tataatggca ggtaacatat ttttagattt aaagtaaaaa gattaacaat
     2821 taatgtcaaa caataaataa gaaaaagact aggtaaaaaa tagcccaatc tgaaactggt
     2881 ttggggaaat tttttgacaa aataacctct ttgaacggca taacgggtaa tttgctggag
     2941 atgaggaatt aaaatctggc ggcgatggtg ataaaccacc agtgagggat gataataaat
     3001 tttttgttgg agttttttgg taagatttag acaaagaaca gtatcttccc ccggccaata
     3061 attagggtca aatttaccgg ctcgattaaa atctgttttc ttgataataa gattgacact
     3121 gggataatca tcgacaaatc tggaggagct aatgcgattg cggtaggtgc cggcgccgcc
     3181 gctgccaaga tatgacgacc agactaaacc ggaagcctgt tgaaacaagt tgtctttttt
     3241 tggggtaaga taggggccac aaaaagcagc gtagtcagga tgttttttga ccaacttggc
     3301 ggcgtttttg aagtaattta gagaagggaa agagtcatca tcgaaaaaaa ctaaataagt
     3361 gccgcgggct tttttggcgc ccagatttct tttttgggaa ggattggcgg tgccggaaac
     3421 tttgtcatca ataactaaaa tttcaaaatt tttgagagat tgagctttga gatgagatag
     3481 agtttcgcga agatatggat ttatttttcg gacggggata ataatggaaa ataaaatatt
     3541 tttggtcatt ggccggtgtt aatccgcata gagagttcgg ggtcataaaa ccatctgcga
     3601 ttgtttttgt catcgtaata atgcaaaatt ctgagacggt aaaaaactgc caaagcatcg
     3661 actaaacatt ggtaaataac tttgataccc tgggcatgag ttaggctgcc aaagtcgtaa
     3721 ttaagcttga taggggcttc ataaattttg gtaaaaccaa ggtggttggc gacagaaata
     3781 atttccagat caaaggcata agtttttatt aacaggcggg gtaaaacttt ttctaaaact
     3841 ttgcgtttga aaattttgag accggcttgg gtatcgcgga tattgatacc aaggagatat
     3901 ttggcaatta aatgaccacc aaaactgatg atttttcggt taagaggata tttgacttgt
     3961 gaggcgggat gatgtttgga agcggcgata atatcggcat cataccactc catatgctct
     4021 aaaatcaggg aaataccgtt ggggtcgatt tccataccgg aatcaataaa ggaaacatag
     4081 tccccttttg atttggccat gccataacga acagcataac ccttgccatg attatttttg
     4141 tagccagtga cagttagatt ttttgattta atgtttttgg ctaaacggta ggaattgtca
     4201 acgctaatac catcaaccac aacaatcagc tcatagtcat agcggatttg atcaagaaca
     4261 ttaaggatgt tagtcaggtc tttggtgata gttttttcct gtttgtagac aggaacaata
     4321 acagagagaa agtgtcggtt acttttcatg gattaggacg gattgataaa cctctaaggt
     4381 ttgcttggca gtatcagacc agttaaacaa tttagcccgg ttgagaccta actgacgata
     4441 ttgacggcaa agcttggggt ttaaccaata ttttttgaga gtggatgaaa tatcgggcac
     4501 agaccggggg ttaaaggaaa gaccagcttg accgacaact tcgggtaaac tggtgtcgcg
     4561 gctaaaagcc acgggacagc cggattgcat agcctgaact agaggaagac caaaaccctc
     4621 agcaaaagaa ggttggcaat aaaccgttgc caggttgtaa ataaccggta aatcttcgtc
     4681 ggagacaaat ccggttaaaa taagagattt agatttttga tattttttgg ctctagcctg
     4741 aagccagcga atatctttgg tccagggatg attgggaaca tcttttttga cggcagagga
     4801 accaacgatc actaaagggt atttgagccg atagcaggct ctaactaaat tgggaatgtt
     4861 tttgttccaa tttatatccc caacaaaaag gataaatttg tttggcagag aatatttatc
     4921 ttttaccgtc ttgagaattt tgggatcagt aatgggctta aaactattgt cggcaccttc
     4981 ataaatggga aaaatatttt ctgaaggata tttgataatt tgggagataa tcttttggga
     5041 ataatgagaa acagtaatga ttttgtcgac tttttggaga aatctttttt gaatttgcca
     5101 ttttagccta cctctgagtc cggcaggaaa ttggtttttg aattgaatag ggatgagatc
     5161 gtggacggtg acgatagtag gtttgacgga tcttggcaga gtaagtttga aggggtcaaa
     5221 ataaggatag tgaataagat cgtatttttt ttgatctaat tttgagcggt cggtgataag
     5281 gtcgatgttg aagttgctgt aagacctatg tttttgggtg tgttgtttga gagatttgat
     5341 gagatttgaa gtgtagtagc cgacaccacg aacagagtta ccatcaacag taggagaaat
     5401 atcaatagcg atatggtatt tttttggcat tattttttgt cccaatcttg ggataaatta
     5461 atatcccgat tggttttgat taactcataa tatttggtgt aggcaatagg aaaccggaga
     5521 gcggaataaa aagaaaagac aaatccggga aaaccatcaa cgtagccgcg gtgtctaaaa
     5581 taagttttaa gaaaccacca aaggggttta aaaaaatagt aagtaataaa acctttaaat
     5641 ccaagagagg ttttgacggt ttttagttct tcggctagaa gagtggtata gcggttggcg
     5701 cgaaggaggt agagagaaaa tgaagtgtcg gcataatgtt ccaaatcatt tttgagccag
     5761 ccgacagttc ctttgacgac ggcttgttcg tgaacatccc gacagggcaa atgagccacg
     5821 ccatttttat aaaatctgat ggttggatcg gggtattgac cgcctttggt aagaaaggtg
     5881 cccaaaaaat aatttttgcg tgggatccaa aaaccatttt cgggaacgga tgagggagat
     5941 ttttgaagat aagatttgat ttcggctttt agcttgggag tgacaacttc gtcggcatcc
     6001 agctgaagaa tccagtcact tttggattcg gatatgccta tttgcttgtt gatgtgaaac
     6061 atgggtttgt tggtggtgga aatgatacgg gcttgtttaa atttttttac caaagccaca
     6121 gtatcatctt gagactgacc atcaaccaca ataatttcgt cggcaatatc tttgatggct
     6181 tcaagacagc ggacaatgtt ttttgattcg ttgtaggtgg ctaaggtgac tgagagagtg
     6241 gcggtttttg acttcatatt ttttgataaa ttttgaaagc gggtgattgg tttgaaaaat
     6301 agataatttt gtccggattg acgggaatat cctcatcgga ggcgacgact agcgatccgg
     6361 tgggaatatc atcccaatta attttaccaa aatgataatt atcgtagccc aaaacggtcg
     6421 agaaaccaaa gttatcagga ggtgtcagtt ggatttggga ttgaatttgt gaaggcggat
     6481 agtgagaaaa aaatagaaat aaaatataag gctgatcgta tttgttggta acgtaaatat
     6541 tttgatagtt gtgtttttgg gattccaaat acggaactaa ctggtcaaac tgatattgcc
     6601 aggcgaaggg ataacgtttg gcatagtgga aaaaataagc atcaacataa taagcaaagg
     6661 aaagcagata aataaggaca aaaaatatgg aaacaagttt tttgaaatac aacttgtgaa
     6721 taaaaatttt taatgaatca aaaagactat aaataccaag agcgataaga atagataaag
     6781 gaatggtcat agacagagct ctgagggcag atggagcctg gaaggtgaga gatgaggcaa
     6841 tgggagaaat gagcagaaaa gaaataataa aagctctgga ttttggggaa tagattttag
     6901 atttgaagag aaagatcagc cctaaaaaca atagcggcac ttcgattagg taaatttgac
     6961 ccatttccgg aacttttgat cgcgggacct cgtcaccgtt gatggacaaa aagtttaggt
     7021 caaaatggga agtatatttt tgagcccaag aaacaaggta aagaagtctt tgattgtgaa
     7081 taattctgtt ggttagtttg gttccctgat gttgattgag aagttcattt gatcgccaaa
     7141 tcggtccttg gtcggcggtt aaaccaacac ctccaaatcg ggcggcacca ccgtgacgca
     7201 ggaaggaaaa taataccggc agggccaaaa taaccccaaa aattacagga gcaattaaaa
     7261 ggggtaattt tttgattaaa aatttaaagt aaataataac caaagacagc gccattaatg
     7321 gagcaattaa acgaagagaa tggtaaaaat aaagtgaaat aacataaaaa aagacgaata
     7381 aaaagaagtt ggataaaaat tttgatgact tgatcgactt aaaaaagtag taagtgccga
     7441 taaccattag gctgagagcg gtttgacttt cccaggcgcc gcgactgaag tgaatgtgcc
     7501 aaggagagat ggcaagaact aaagaactat agagcgataa atttttggac ttagaaatta
     7561 acaaaattag tttgtataaa aagaaaatag tgagaaggga aagaatcagg ttgggtagac
     7621 ggactgccag tggggtaaga ccaaaaattt tgacaaaggg aatggctaag tagacatagc
     7681 caccgggttt gtaatcgcca aaagatttga agtgaagcgg ccaggagaca ccgtgttcat
     7741 ctttgccggt ttgaatgaga gaataagcgt tatagccgag agcggcttcg tcgggattga
     7801 gagctggagt gtggtcaaga ccgataaacc ggactaaaaa agcaacaatt aaaataaaat
     7861 aaattaaata tttatggatt ttcataagca taaaaggtag aaatttgacc aataggactt
     7921 aggggaataa attggctaaa ttccggtttt ttatcaatcc cctgccggta aatttcttcc
     7981 tgagaaaaaa tagataaggt gcccggatgt gagttggtaa attcaaagct gggggtagaa
     8041 aattttatat tatcaataga ttcaactttg ccggtatcaa gagaagattc ggttagattg
     8101 gcaatttgct gatagtctga tggtgagtaa cgtgagtaaa aaagataaaa aatgtatggt
     8161 tgaccataaa agtcgctaaa tataatttgc tgatatttag actgagaatt attgacataa
     8221 gaaacagcct gtttgtaacc gtataaaaaa tcatagggcg actttttaac catatgatta
     8281 agataaagat cgctatagta gacaaaagaa ataaggtaaa taaaaataat aaagatggga
     8341 attaattttg atgggtatct ttttagaaaa gcaaagatcc cgagagagat gaaatagatc
     8401 agaggaatgg aaagagacat agctctgact gattggattg aatcccgagt aagggcggaa
     8461 ggaatggggg cgaccaacag ccaaagaagg aaaaataaat ttatagattg gtttttggag
     8521 cggctaaatc caaaaaccaa accgataaca agaaaaatta aactggggta taagagaaca
     8581 ccgatatagg gagcggagtg gcgggggttt tgccagtcac cttcaaaggc caaaaatcgg
     8641 ggagaaaagt ggttgaaata tcgagacaga aaatttcgga taaaaaatga tggctggctg
     8701 tgaaaaattt gataatcaag atgattggtt tcattaataa tggtattggt ttcaatttcg
     8761 aagcgggggt aggaaaataa actgacaact tgaagacggt tggcattgct gctaaaaaat
     8821 aaaccataag caacgggcag ggcaattata cccaagggga taacaaaact taataaaaat
     8881 tttttgaatt gaatttgttt gaaatttagg ataaataaaa taatgattaa aaataagctg
     8941 atcattttgc ccgcttgata ggtaaaaagt gaaagtccaa aaataatggc agaaaagaat
     9001 aaatattttg accgctgacg gttgatgtat ttgtaaaaga aatatgaagc taaaataagc
     9061 tcaaaagtga gaatattagt ttcccaagca ccgcggctga aatgaatatt ataaggatta
     9121 aaggcacaaa tgagagcggc aatagaggca attttttttg atttagggga aatttgagtg
     9181 attaaaagaa agagaaaaac cggagagaga gcgccaaaaa tcatggatgg gagtctgact
     9241 gaaagttcac caagaccaag taatttaacg aaagggagag caaggtagac gtaaagtccg
     9301 ggtttgtagt cgccaaagga tttgaaaata agaggaagaa atttgccata ctcatctttt
     9361 agagtttgag aaatagaata ggcgttgtag ccgagagcgg cttcgtccca aaggagcggg
     9421 gttttttgcc agaaaacaaa tctgaccaaa aaactgatga gaaaaatcaa aaataaaaat
     9481 agccgatttg aaactgattt agtcataatt tatttttagg atttgaaaca ccggcgagcc
     9541 gtcaggataa taaatagttt ttagtaattg actattatgt tgattgtagt tctcaggaga
     9601 ggtaattaaa agtgtatttt ggtttttagt gagagttgga gtgatagttt tgtcttttat
     9661 ctcccaatca ttgataaatt ggaatttatc aaacttgtcg acccaatacc aattggcgtg
     9721 atagtcccaa atcttagcag agtcattttg atagacggaa ggattccaag gccagtaaaa
     9781 gagaaaaaat tcgtgaggtt caccatattt tttggtgacc aaaatttggt cgtagtctga
     9841 ataaattgat ttgacgtaat caaccgcctg ttggtagccg tattgccagg atgaggcgta
     9901 tgtttgacgg tattgaatgt atttgggcca aaactggatt tggcttagaa ccaaaacaaa
     9961 agtcagacag aaataagaaa atttgggtcg gtagaaatca agacgagaaa aaccgatggc
    10021 gataattatg atagttgagg gaataaaaag aatacttctg agaacgtggg gagcgtcacg
    10081 ggtgatggct gaagggatag gagaaaccaa aaaccaaaag agtaatagtt gatattcggg
    10141 atggttttta aaatttttga gcagatggat aaggccgagt attaaaaaag gaattaacag
    10201 agatgaaaga agataaaatt gaggaatatt gaattgataa tgggaaccgc cgttaaaaaa
    10261 taagtaaaga gggttaaagt gtttaagata atttttgccg gcagtaaagg taaaataagt
    10321 gagtttattg acaaggaacc ggggataggt ttgtcgaagt tcgttgatgc gattgataga
    10381 accgctgtca agaagcgaaa cccactggta acgtgcctga cctgactgat tgaacatctg
    10441 ccaaatagtt aatagagtga gcaataaaaa tggcaaaaag cggtaaaaat tctttttgag
    10501 atggtattta tgtctggcta aaaaataaat taaagagaga ataaaaaatg gcagcaaaac
    10561 ccggctactg ttgtaagcaa ataaactcag accgtaaaca aaagcactca agggatattt
    10621 gcgactaatt aaaagataaa aactaagaca aaagagaagg gcaaataaat tggcctcaag
    10681 ggcgatacgg gaaagaaata tagtgccggg agataaaagg gtaataaggg cggcaaagag
    10741 aggtaatttt tggttttgag gtaaaatttt tttgacaata agataagcta aaaccggaat
    10801 caaagaaccg gcaataagag aaactaatct gacagaaaaa ggattgagac caaaaataaa
    10861 aacagagggt atggttagat aaatataaag tggaagcttg tagtcgccgt aggctctaaa
    10921 aatcagaggt aagggactcc cccactcgtc acgaccggtt ttaagaagag aataggcgtt
    10981 gtagccgtgg gaaacttcat cccaattaag actaggaggg aatttgttga tataaattga
    11041 tcttaaaata aagccaaaaa caaaaatcaa gattaataat agtttgtccc gtttcatatt
    11101 ttggtgtgat aaatataaat tgccggttgc tgccgagaat ggtcggggta atagattata
    11161 tgatccggtt tgacgtctct aaactcttcg gggcggccga caataatagt attaggaatt
    11221 tggctgtcgc tagggatgtt aatttctttg aatataaatt tgtccagttt atattcgccg
    11281 agttgatcca aaaacataag atgactagtt tcataatcaa gccaattgac tgattctgat
    11341 tggataattt tgggattaat ttgattaaaa aaggcaacat aagcttgagg ttcagataat
    11401 ttgcgcgaat aaataatttt gtcggcgggg tgattttgga gatactcatt ggcttgttgg
    11461 tgaccataga gcataccgtg agctaaaatt tgattggatt gataaaaata tctagctaaa
    11521 aaagtgccgg tagttaagat aaaagtaaat gtaaaaataa gggataaaat tggtgaaaat
    11581 tttttgggga cttgatttag aaataagacc agaccggcgg cagagaaaat ttggataaac
    11641 ggcatcatca gagatacccg attggcagaa tattgtcctt tggcaatggc tccgatggcc
    11701 ggggagatga gaaaaataac cagcaggaga agcagggatt tggacgggtc ggatttgggt
    11761 ggaaatttaa tcaaataaat tagggcaaaa acaaaaccga tagccggaat aaggcctaag
    11821 actccataac cgggaatcat gccgtaggtg gtttcaccgg caccgttggt agctaaaaat
    11881 tgcggagaga gataagaaaa ataatgatgg aaaaaagttt catataaata agtgactttg
    11941 ttgtataaaa cccgggaaag ctggggttga agaccatttt gacggatttc atattggctg
    12001 ttggagacat attgccattt gtcagtggga ttaaagatgg cgatgtcgcc ggttcgatct
    12061 gactttattt gactgttgag ataaaagttg aacgagaaaa aactaccaaa gataatgatc
    12121 aaactaatca aatatttttt gtgcttataa agggaaaaat aaaaaaataa aattacaagt
    12181 aaaaccgggg taaatatttt ggcagaatga taggtaaata aacttaaacc aaacaaaacg
    12241 gcagagatgg aaaataaaaa taattgccgg tttttgagtg aggtgataaa caaaagcagc
    12301 gctagactaa cgacaaaaac gaccaaatta gcttctagag caatgcgaga cataggtaaa
    12361 ctccagggtg agacggccat aaaaagactg cttaaaagag aaattgatgg ttgatgaaat
    12421 aaattgtgag caataagata ggtgataaaa atggtaacaa cagctaaaat tgcgtttggt
    12481 aatctgacgg caaaaggagt gagaccaaaa attttgatac tgggaatcat aagtaaggtt
    12541 tgaagtgggg gtttgtagtc gccaaaagac ctgagattta accagttttg actcccccac
    12601 tcatcttggc cggtttggat aatagaataa gcggtgtagc cttgagccag ctcgtcggga
    12661 gtataagaat ttgggagaga ccccaattta taaaatctta aaaaggtggc caaaactaaa
    12721 attaaaacta aaataagctt atgtctcata atttttggat gatttgcgcc aaaatagtat
    12781 ggttggggtt gtaaatggtt ttgatggtgg taaaaccggc aagcggttgt tgggaaaaat
    12841 ccatgtcgcc ggggacatct ttttcttgaa acaagacaaa aatatcatgg gacttagctt
    12901 tggctttgac atcttcaaga tgatcgatcg atgaccattg attaatgaag gtggtttgag
    12961 agttaaggtt aaactgaggg ccgttgtcaa caactttgtc ggcaataaag ttttggggtg
    13021 aatatttttg ataaaaaaga taaggcaaaa gcgaattata tttggcgttg ctgatataaa
    13081 gatggtttga aattgagggg atatttttga atatttgatc gtagccgtaa ttccaatttt
    13141 caaatgaatt tttgggataa tgaacaaaat attcgtgggc aaaagaaata atttggagag
    13201 ataaacaaat aatcataaaa ctgtaaacaa gacgcgattt ggcaacagga attttgatga
    13261 taccaaaagc aataaaataa gccaaaggaa caaccattaa aaacaatctg gtggcatgag
    13321 tgccgccacc caaggttaga ctggagggaa tgggagagat aataagccaa aataacatca
    13381 atttatcaag gggttttttg ggcgacattt taaaaagacc aaaaagcaaa aaagggagaa
    13441 aaataataaa aatttgacca aaattgggaa tagaatgacg aacattaaga aaatctccgg
    13501 ttaaaaacaa gaaatctaag gaaaatgact taatataatt ttttgaaatt tcggttagat
    13561 actttccggg tttatggtga aaaatagctt caatattggg gttggtggca gaaaaatcgg
    13621 ttcttttgac aactatttga tcgataactt tggggttgtt gataatgctt agagatgaaa
    13681 atctggcggc cgcctgacca taaaaaattt ctttaacaag agggaaggtt agcactaagg
    13741 ttaagattaa ggcggtaaaa attaaagacg gtttgagtcg atgaataatg gtttttaggt
    13801 tggtcaagat caaaaatatg accaaaagag ggacaaatat attagcggtg ttgtaggtat
    13861 aaaaactaag tccaaaaaat aaaattgaaa gataaagaaa tttattttta gtattgttga
    13921 taaataaata aaaaaagtaa gtgccggcaa gaattagact aattaagaga gtgacttcaa
    13981 aagcagcccg gctgtaatga aagtgccagg gcataagact gagaacggca gcggaagcta
    14041 aagataatgg tttggactga gacatagaca aaaccagttt atagataaga taaatactca
    14101 gaacaccaaa caaaaccgga gcaagacgaa ctccaacttg gttgagacca aaaagactga
    14161 caaaaggaac aacagtataa attagaagcg gagctctaga ctcagctaag gattggagat
    14221 aaaaaggcag aaaattaccc ttgtagtcgc ggccggtttg agacaaagaa aaagcttgat
    14281 agccgacatc aatttcatca ccaaaaagac tgagattgtt gagacggtag atcctgatta
    14341 gaaaagaaat aataataata gcgatgattc cccaagaaac tggtttagag gtcacttttt
    14401 agatatttaa ccaagtaaac aagacaagtt actagtagta taaacgataa tagccggcaa
    14461 gtaaggaagt tgaagccaat aagcagggtt ttgagaggag aaactagggg ataaaaccaa
    14521 agattgtaga gatttaggaa atgaattaga gataaagcca aaaggtgttt gaggttaaat
    14581 tttgattttg gcagaaaaat aattgtagta agaaggggaa aaacagggta aagataacgt
    14641 tcgtgcatat tggttaaaaa tataaaactg ccaaaggcag acaacattag agcataaaaa
    14701 agattagtca gggttagttt ggatttgata aatttaaacc aaactgggag aagaaataaa
    14761 agatataaca atctccccca ccattgataa gttagacccc aaaatttagt aaattcggat
    14821 aaggataaat ttataccgaa taatcctgcc caaaaattga aagcattgcc gtttagcata
    14881 ctcccttgcc gaaccaaaac ccgattggta tacatatacc ataaccattc aaaaggattt
    14941 ttgtcgccaa aagcaaaagg gatggccaag acaaagatta atacagcaaa actaattacc
    15001 ggagcaataa attttttcca atctttaagt ctttttagaa ataggagacc taaaataggc
    15061 agatagataa taagtgataa tttaaacaaa aaccctgaca aaaacaaaat gacaccggaa
    15121 aaatattttt tttggtaaat caaataaagg ccggcgagag ctaataaatt aatcagagaa
    15181 tcggtttgtc cccaaatggc agagttgtaa attaaaaccg gattaaaaag aaagatagtc
    15241 gcggcagtaa tggctttgga tgggtgatat tggtggataa ttttgtagat tagataaccg
    15301 atgccaatat cggagagaat aaatggcagt tttaccagcc aaacatgaag attttgttct
    15361 agaaccggga taataaaaga agggaaaatg ggaatagtgt tgttgagaaa aaggatgatt
    15421 tggaacagaa acttgttgat aacggcaatg aaagcaaaca gcaacattga acccaaaggt
    15481 tggttgggcc aggaaacacc ccaaaaaata ttctcataaa atttttttga ccctaaagac
    15541 aaaaatctaa ctccccaatc aaggtggttt aaaatatcgg gatgataacc ggaaataatg
    15601 accaagataa tcctaaaaag taaagcaaaa ataagaatat tttttatttt tgaaaaagtt
    15661 tttgccattt ttgtcctgac cagattataa aatttattag gtaatgttta aagtagccgt
    15721 gaaatttgat actgctggga ataagacggc gccattggtt tttggcatca gccagacttt
    15781 gaccgctaaa gccatgacga tggataatcc ggcacttggg atagtaataa acttgccggt
    15841 ttaaccggtg aacggcgcgg cagagatcca aatcttcgta ataaaaaaaa tatttttcgt
    15901 tccagccgcc gatttggtta aaaatagatt tagaaattag cagagcgccg ccggaaatat
    15961 attcaaccgg acagggagat ttggttttgg gggtatattt agaataagtt ttttgaccca
    16021 gccaaaattc cttgaaggca ttagcaatgg tttgagaagg aaagactgag gcttgggagg
    16081 tcatgtcagg gtttaaaaat ttggggccga tacagccaat atttttgtta ttttggagat
    16141 aagcaaacat ttggataaga ctttgatcaa tcagctccat gtcggagttt aaaaaaagga
    16201 aataatcact tttggcaatt ttggcagctt gattgttggc tgtagcaaag cctaagtttt
    16261 gtttgttttt gatcagccgg acgctagtgg gagttagcaa cttaacggta ttgtcgatgg
    16321 aaccattatc gatgataata atttcgtagg gaaaattttt gagatgtttt tggatggttt
    16381 tgacacactt cagagtgacg cgggcagtgt tccaagtggg gatgatgata ctaattaagg
    16441 gtttcatttt ttgaattcaa taaggctaaa tattttttgg caattaggaa aaaaataaat
    16501 aaacttgata aggataagag acgaataaaa ataaagtcat tattcttaaa aaaatgagaa
    16561 atgaactcta tgtctcgatg atagtaagca taaattaaat ttaaaaacca aatcagttgc
    16621 tgtattaaca ttaacttacc aaatttttgc caataaagcg ccataattat taaagatgat
    16681 aaaaaagtgg gaaagaaata tctctcaagc attccggtca tcagcaagaa ggcaccctgg
    16741 gaaataaaaa aaatagtaaa taaactatgg ttaaagttta ttttttttga cctaaaaaat
    16801 ttagagataa aaaatatatt taataaaaca aaaaatgacc aacttatgta agttaatttg
    16861 aaatgcggcg ttatggcaga ataggttttg tcgatagtaa atactaatga atataagtta
    16921 aaagctgaaa ctgaggctag ttcaacacct ttagaagaat tgctgatgcg agaatcaaaa
    16981 attgatctta tgtattgaag atggttgtgg tttttgggaa taaaaggcaa aaatgataaa
    17041 taaaatatgg ctagagataa aactaaaggt ataacaatat ttttaatttt atttaaagta
    17101 ttcccctttt tagtatttaa atataaatat aaaattccaa aaaaaggcaa gataaccact
    17161 atagtgggtt tgaataatat acccaaaatg aacaccacgg gactgaaaat tttttgtttt
    17221 gtaaataaaa ttaagaaagc tatatatatg aaaatagtag ccaaaaggtc attttgcccc
    17281 cagactgagt tggtaaaaaa caaaatgggg tggaataaaa ttaaacaaaa aattattaaa
    17341 ccgatgtttg gggatttttt tgacgagtaa tagaaataaa aaataaaagc gataataaca
    17401 gtggctaaaa attcccaaat ttttagggtt agtatttgac cgtattgctg aaatcccaac
    17461 aaaataaaag ctggaggaat ttttatgagg ttatgcatgg cagagaagat gtttctgttt
    17521 tcataaattg atctggaggc cgaataagcc atcatcatta atgatggctg agtgggtgga
    17581 gtataagtcc agttgttaaa aaaataagaa ccggttagac cctggtggta taaaactttt
    17641 gaccactcct ggtggactaa aatgtcgcct ttaggggtag ataaatttga tatgtaaatg
    17701 cgactaataa aagaaagact gaaaaatatt agaatcaaaa tatttttttt cataacggaa
    17761 aggataaatt tggagaaaaa tttccccaat tggagatcat atataagaga gactgttggt
    17821 tccatttatt gacttgataa aaaatcatta atacaaaggt taaccaaatg taataataaa
    17881 attttgtaac ttttttcttt ctaataaaga aaattaaggt aaaaaatagt aaaaggatac
    17941 gataaataag aaaacttttt tgtgaatcat gaaaactgct gtagccccaa aaaaaggaag
    18001 aagaaaaaac aagtaaaaag aagagggtaa agttacctaa atttggaagt attacgagat
    18061 tataaattaa gacagatgaa gcaataataa taaaaggtaa aaagggtaag taataccaag
    18121 gatagtttag accacctaga aaaagaaata taaaaagata aataataaaa ggggtaagta
    18181 aaacaatata ctttttataa ttttttatag acaaaataaa gatagatatt atcccaaata
    18241 accaataccc actttttgga aaaccagaaa aattaggttg aattatttga tgaaagaaag
    18301 aaagagatcc aaaccattcc cgatgagcct ggctaaaaag aagtttaaag aataaatccg
    18361 gtgaaagata atacatataa atgtaataac tgattgaaat taaaacaaaa ggaataatat
    18421 aagaaaggaa agtttttttg tttatttttt ctttaactaa aaatattaat cctgttaaga
    18481 aaataaaaat tcctgactct ttggtgataa atgacagacc agcaaatagt ccagctaata
    18541 acaaaaaaat atattttgat ttatatttat tgaataaaat aattaatgag aaagtgatta
    18601 aactaagagg aataagaata ttctcaaaca aggcaaaccg agacattaga acaaattctg
    18661 gaacattcga gtaaaggaaa aaggatagaa atgaaataaa ataatttttg aataataaaa
    18721 atgaaaacaa aaatatagaa aagccggtaa taactgaaat gattaaagaa actttcctat
    18781 aatcagaggg tttaatatca tcaaactttt caatattttt aatgggaagt gaatataacc
    18841 aacttccgaa gattggatgg tcaaagaatg gttgaactat tcttatggtc tctttacctt
    18901 tgccaatatc aacgtctata acggtggtaa ctggataatt aaataattgt ttgtttttaa
    18961 gatttggttt ttgaccttgg gtatttatac taattccatc gaaagaaaga tttgaattgt
    19021 cttttttgtc atcaagttct ttataaatct ccatggttga ccaacctgta ggaactccgg
    19081 tttttctaaa actatagcca acgaacggat agtcgtgttc atcaaaaata aaagtattag
    19141 gaatggcttc ataccctatc cacttattaa aaaaaaactt atagcttaat aggcttaaaa
    19201 tcgtaaacat taataaacca attattatat tttttttgtt catgaaatgt tttgccacca
    19261 aaaatcaata tttattttat ttaaatagat taaatttaga tagattgaaa atccaaacaa
    19321 aattaaggaa attgctgttt ttagattttt atttattttt attttattta ttaaaaaata
    19381 aataattcca ataaacaaaa tgaagctgac tccaaatcgc cagtattttg agtatggttg
    19441 gaattcaaaa attcccacaa ttcggcttat gttaactcct aagactatta tcaagttaat
    19501 aatcaataag tagaattgcc tacatttttt cagagaagta atgaaaaaat gagaaaaaac
    19561 tatgataagg attggccaaa atggaaaagc ataccaacca taaggctggc tgccaaaaaa
    19621 tacataaatt attaaataag agattagagc tacaaatgga actatgattt tatattttat
    19681 tttcttttta agaaaataag taaaagatac tatccaagaa acaacgatcc aaacttcggg
    19741 taaaaatttg tgttgggtta ggcgttgttg acggattaaa tcaaaaatgg cactaggacc
    19801 aatcccatag aaacggtgac tatttgataa aaaaatattg aaaaaatgtt taagaccata
    19861 aacagatcca tatattaaaa acagggaagt aactggtagg gcaattaaaa ggtaaaagaa
    19921 aaaatcttta aaaaactgtt tagtaaagtt gtgatagtaa tttaggagag ataaaaatac
    19981 aaacaaataa caaacaaaac ctgttaactt aaataaagta gctactccag aagctaaagc
    20041 cgcaaggatc aaccaatgat cttttttgct ttttatgtat tgactgataa aaatcattgt
    20101 taataaaaac atagggatga tggcattttc agattgaacc attctcccac ttagaactat
    20161 cagtggtgta gtcccataaa ttaaaccgct gacgatagca gataaaaatc caaaatttaa
    20221 ccaacaataa atcattatta aagctacgct tatggtagat attaaaataa taggtctacg
    20281 gataaataag gcaatcgtat cttcaaaaac acgagccccc tgagtatagg cgtaaccgcc
    20341 agttatgaga cccaaaagcg gtggatgatc catccaagga taatttagtg ttaaaggatc
    20401 acttgataga tattgggtga acctgtcaac gtttacatat tctttagtct cattttgata
    20461 gtttggtaat cctgagatgc ctattggcat gccggtttct ataagagata accctaccca
    20521 agaatttgaa tattcgtcgg ttgactggcc ggggatagga atttgagcat aatcacgttt
    20581 ccttaaaaat aaagataata tcgcaatgca gatgagtatt atcactttaa tactattttt
    20641 caacatatcc acgaaggttt ttatcttgat aacaattaat agtagaagtt ggtaaaattt
    20701 tttcaaaaat atctttattg ctaatttctt tgtcattata aaaacaaacc catttttgtt
    20761 gagaaattaa atctttatca aaatttatta ttctggaata ttctgaaaaa ataccatcga
    20821 tcgttgggtt aggaatagct attaaagcta aggtacttcg agatgatcga ctatcagtgc
    20881 accaaaaatt aatttttaca tactgatccc ctacttgagg gtcgcccagt attttatatt
    20941 tacataaatc gtcagtttta tctctgaatt tagaaacagt atcggttaga tatgataatt
    21001 tttcaggaga aaaattttta tttttatgtg ttcttaaacc aaaaaaaatt aaaaaaccaa
    21061 ttaaagtaaa tatggataga tatactaata aaaatttttg gcgattcata aatttattta
    21121 aatatatcca gcaactccaa atcggtgagc ttaggaaaga cgatgggagc ggggtgtttt
    21181 tgaacttgtt ttttggcggc acggataatt ttgacgtaat ttgggccggt caaaacccga
    21241 ccgactaaag ctagtttatt ttggaacaat aatttggggt cggtgatatt gcgccaggtg
    21301 ctaagcaacc gatttctttc tttaacataa ttgagtaaat ttggagaaaa tttggacata
    21361 gttgattcgt gatggtgctc tacctgactt tttggctccc agagaagggt gtaacccgat
    21421 ttccaggccc gataacctaa atctaaatct tcggaataaa aaggatgata aataggatca
    21481 aagccgccta atttttggaa aagatttttt ttgacaatag aactgccgcc ggaaacccag
    21541 ccggaaatat gagttttgtt caaaccggtg gtgccgggct gatgttgaag atagcccccc
    21601 tgccaaaaaa atcgagccca atttttgttg ttggtttcac aaaaaccgac tccaaaaact
    21661 ttggggtcgg aaaaatgttt gagtgagtga gagatatagt ttttttgagg aataatatcg
    21721 ttgtttaata agacaactaa atcacctttg gcctgagaaa cggcataatt ggagttagca
    21781 ccaaaaccaa gattgttttg gtgagaaata attttaagtt ggcgatattt tttttggaga
    21841 gatttgacaa actttaggct gtcatcttcg gaagcgtcgt cggcaaaaat aacttcaaaa
    21901 gcttcggggc tatttttgat aacaatttcc aaattttttt tgagtaaact aagaccgttc
    21961 cagttggtga caataacaga tattttcata ttatttgccg ataataccca ttagtttttt
    22021 ctcgaaatta gatttggaaa attgttggca gttgatgagc gattgctggg acattagttt
    22081 gagaatttcg gggtcatcaa ttaacttttg ggttttagca accaattcgt caacagtttt
    22141 ccataaaaat ccactgacgc cgttgtcaac aatttcgggg ataccaccct gttcaaccac
    22201 caccggcacg agacctgagg ccataccttc gacaacggtg ataccaaaat gctcagtatt
    22261 ttcggggtga acggtttcat ctacttcaaa accggcagca tgccaataga ttttggcaac
    22321 ggaatacaat tcttggagaa cattaaaagg cgggttaacc ataaattcaa ccgataaatt
    22381 ggcagccttt ttttggagat attggagaaa agaatttttt tcgggatcgg taagactacc
    22441 accggctagg actaatttcc agttgccgat gggagatttt tggcttagct gtctgaaagc
    22501 atcaaccagg acatcctgtt ttttggcatt aagaatgttg tcaaatcgac cgacggaaag
    22561 aataatattt tctttgcttt tgctggcgta aaatttatcg acatcgaccg gcggataaac
    22621 aactaaaact ttatttttta cgtcttgatc ttggtatttg gcggtaaatt gggaattgca
    22681 aataatgttg gtaaatagtt ttgatttgag acgacggaag attcttgagg ggaggtcata
    22741 atgattgaca aaaggcactt gaacatgaag ataatttttt ttgccaaaaa ggaggggaat
    22801 actgccgtcg ctaagataaa aaagaacatc atatttacgg ttgtttttta tttttttgga
    22861 aatactactg agtttgacct tgagacgaaa agggtcagct acccgattgg taaacttaga
    22921 caaatcatcg tgtgtttcca aaaggttgag attttgaggt ttgataccaa aaatatcggg
    22981 aaccattttt agctctttga gatcaagaga gaaacgtttt tcagcttttt ctatcagagt
    23041 tttgtcacca gaccaaaaaa tgtcaacaga atagccgttt ttgatgagaa tttcggctac
    23101 tgtcagacaa tatctttcac cgccacctaa agtatctaaa taagggtcat aaatcgctgc
    23161 ttttttggta gccatcaact taatgatacc ttaaattttc tgattttgct tttgttgaag
    23221 ctcccagaga cgggcgtaaa taagaaaagt attaaaaact tcaaaaataa cggaaatcca
    23281 accaacctgg ccatcgcgcc acatttgctg tttgatgaga cgctcccaag ctttggttag
    23341 catcattctg ataaaccgcc accaaactac cggcggatga tcggctttga aaagggcttt
    23401 ggcttcggtg tcggtccagg ctaaggtctt gttaaccatg gaagtgagat cacggtgggt
    23461 ataatgaagt aaatcattat tgatataacc taattctccg gtaataatag gttgttcgtg
    23521 caaaactcct ttgtaaccgg taaagaattc ccggttgaaa agacgtttga cataatcagg
    23581 ataggaatca ccataaaaaa ctctttggcc taaaaaatgg ttggctcggg agatggcgaa
    23641 gtgagtaaat ttgggaggtg aagaagaaac gatttgagta atttttttta ttagattggc
    23701 gggaactctt tcgtcggcat caacataaaa tatccagggt tgagtgctgg acttaaaacc
    23761 gagattgcgc cacttgctaa aatttttgtt gtattcatct tcggtcctga caattttgac
    23821 ttggggaaaa tgctttttga ccaaaggaac agtctgatcg gaagaattgg cagcaactaa
    23881 aactatctcc tgaacccaat tgcaggtttt gagacagtca agaatcatgg tttgttcgtt
    23941 gccagccaaa atgataatag agataggtgg tttggttatt gccatgatcc tttggaaaat
    24001 tttttgaggt aaaaatcggt gatgccgatt ttttgccatt tggctacttt ggggtcaagt
    24061 aatttggcaa ttgattcttt gatgagagca attttagtcc taaaagaagc ataacgaaaa
    24121 ccaaaaagaa gccggttgcg gctaagaaaa taattatgca aatcaccgcc gctaccggaa
    24181 gatccggcat taacatgcca tatttttgat gaggggatat acgccagatt gaatccggct
    24241 aatctagccc ggcgacaaaa atcggcgtct tcaagataca taaagtattt ttggtctaaa
    24301 agaccaattt gatgaaaaac tgacgatttg accaaaagac aacagccggt taaaaaatca
    24361 accttgttgt taatatcatc aaattggcca gagtcaactt catcaacacc aatatggctg
    24421 ccataaatat tgcgccaatc gatttgaccg cctgccgacc aaatgacccg accgcgatct
    24481 tttttggtat atctttggcg atgaaactca aatccggggg caaaataaat tttggggccc
    24541 aagatatcat aatctttggt gtgacgtaaa agattgataa ggaagtctgg gtcaactaga
    24601 acgtcgttgt tgataatcaa aacataatca aagttgtttt tgagagcata atcgatacca
    24661 aaattattgc cgccggcaaa acccagattg gaattggtag taataatttt gactttggga
    24721 tgagagtgat agcggtcgcg aaaaatagtc agagaattgt cggaagaacc gttatcgatc
    24781 aaaataattt gataatctat ctgacggaag ttaattttga ggatagattc gactgaattg
    24841 acggtcagat ccggctgatt ccagttgaga actaaaatgg cagttttata cataaactag
    24901 aaatttttgg ttaaacggtt tagacggtgg cgtagtttta tgagtccagt tttaaaaata
    24961 cccggcttat attcattttt gaaaaatcgg gtaaaccaat ggtcatattc aaagactgac
    25021 tcttggctgg cagaaataaa ggattctttg ggcaggggtt tgtcttgaga accctgaatt
    25081 tcccaaagtt tcatataaat aagacagatg gcaaaagcct gaaggagaca aaaaacaaga
    25141 ccgtgaagac catctttgaa accttcgcca aaaaagaaac ggctgttgaa ttcctgaacc
    25201 ggtttgagca gaaaatcact aattttgact ttataatctt tggcatttag ttcttcggcc
    25261 tgaatgctgc tataacgcat ggctctgaga acaaactgat ttaggttttg ataattattg
    25321 tgtttgatgg caagtttttc ggagtcaagc aaggtgagac cgttgccttt ggtaaccggc
    25381 tgactatgaa tttctttttg ccaggtgaca gcaccttttt taaagaaacg gatgaggtaa
    25441 tctggccagg aattggcgtg gcgaatccat ttgccaaaaa ttatgttggc tcggggaatt
    25501 ttgacaaaat caacatcggc acggagagta atttttttta gttcccgttt gagagttttg
    25561 gaaagatact cgtcgggatc gagaagcaga atccattttc ctgaggcttt tgagagagca
    25621 aaatttctgg caggttcaac atacttcatc ggccggtaac taaaaacctt ggcattgtaa
    25681 cttttggcga tagcgacact gtcatcggtt gaatgcatat cgacaacaat aatttcatcg
    25741 acaaaatctt tgatggattt aaggcatttg gatagatgtt gggcttcgtt gcggacgttg
    25801 ataacggcag aaacgttaat ttttggcttc ataagttatg atagtctatc attttctgac
    25861 ctgataaaga cgaatttgtc cttggtcgta aatttgatca atctgaagat cttctatagt
    25921 taggggtaat ttttgctgat tggttaaata aatataatca atcttatgat tgagtaaaaa
    25981 tccccgggca taaaattcgt cggtggtggt aaaaaatttg tcaacttcat gacggcgggt
    26041 ctgccagtcg aaaccagtaa tatcaaggtt catttcgtct tctaaaaacg actggtggcc
    26101 gctaaaagcg gaaacataag ccgtagtttc gtaagcataa aggggaatgg gggtggacat
    26161 atcagttttg agatgggggt cgtagggata ggttaaaaca ataccctggg gttgtttttt
    26221 gagaaaattt aaagcctgaa tttcggaaga tggcagggaa gcggggggag ggaaacccaa
    26281 gtaatctttg atggtggcaa aactggtaag acaggcaata aataaaatga tcaaagaagt
    26341 taggcgatat ttttgccata aatgggagag aaaaatggca aagtaaaaat tagcaaaaaa
    26401 caagccgtaa taaaaaaatt gaattgtatt ccaagcagtc cccttttgga caaaaaatag
    26461 aggaatagca aaagccaata aacaaattga tagggttaga aatttgaacg atgattgagt
    26521 ttttgagtgc tgatggttga gagaaaagat gcccaaaaat cttagtccaa ggttgccgat
    26581 aaggaaaata aaaattaaac ccagctcaat aacggcagaa attaatttat tggtttggtt
    26641 gtagcggtag gaagcaagcc ggggccaata aaatttatca aaagattcga tcatggagtg
    26701 agtaaaccaa aaaggcttga attcaatcag agaactgctg tttagcacgc ccaacaaagc
    26761 caaaataatc agagaaacaa aagccatagt taaacaaata acggcattaa acttaaattt
    26821 tggtttgtca aaaaacaaat tataaagaca aagtaaacta aaaccgagac cgacgagaat
    26881 gccagcataa actttgatac cgctaagaaa cccaaaaatc aggccgataa tgacggcata
    26941 tttaggggac ttggtttgat atttttgcca caaaatcaga ccagttaggg tgagaattaa
    27001 agatagagca aaaggtggat ttagcaaggt tgaggctgac tgcattgacc aaaaaaggga
    27061 ttcgccgcca aaatggcgat tgaccaacaa agtatatatc caaccaaagg agccggcaaa
    27121 atagtttaaa atgacaaaaa ataaactaac tctattattt ttggtgatga gataagccag
    27181 tttgtaggac aaaaaaccga taagaagagc aaaaataaag ggcagaattt gaaaatataa
    27241 aattgatggg tttaaaaaag taaatttgga gataagagcc accaataaat caaaacccca
    27301 atgatattga gagagagtgg tgccggaaaa aaccgggttt gggggaggaa tttgctgttt
    27361 gagctgattg attagactga gatgccaaat accatcatga ccgtttgggc cccaaaaacc
    27421 caggccaaaa tcatatttaa gaccgcttct aaccatggtc aggctaagaa taagactgga
    27481 gatcaagatg gtggcaatta ctgatttttt tgtcttaaac ataataattg ttgataaaac
    27541 cacctaattt catttttgga aaagagaaga tgaaaaagaa gataaactga aacggaaatg
    27601 actaccttac caataaggct gacccaaccg taaaaaccaa aattctggaa aataaggcta
    27661 aaaataagca ttaaaatagt ggctaaaagt ggtttggaaa tagctgacaa aacccggaca
    27721 ccaaatattt tttgggcaaa ataccagaca ataaaagagc tggaaccaac caaaagagtg
    27781 gcataagcag tgccaagata accgaatttg atgcttaacc aaggaaataa aatccaagtt
    27841 aaaaccgtcc acataatcat aaatttggta gttagggtga ttttgccaat ggcattgaaa
    27901 gcgttggtaa tgggagtagt gatagcagca atagcaacat tagcggcata aaaataaagc
    27961 ggaaaaatag ccggcgccca cttggtatat ttgggaataa tattgatgat gtcgggagaa
    28021 ataagcgtaa tgccggcaac cataggaaaa acaaacaaag caataaaata taaacttctt
    28081 tcgagagatt ttttgagaat atctttgtga tcttggagac gggcaaaggt aggaaaggtg
    28141 actttcatga tagcgtccat aaaacttaga ggaattcgcg gacctttttg agcccaagag
    28201 aggataccaa aagcttcacg accgatgatg ccggcaacaa agaggctaga tagtctgtct
    28261 ttggccatgg caataaaaga atttaattga aaaggaatgc catatttaaa aagttccttg
    28321 atagatgccc aatcgagagc aaaaccaatc ggccagcggc tataacggta aatcaaaact
    28381 aaacctaaaa gactgcggac aaaagtggcg atggcatagg aataggcccc aaatccaaaa
    28441 taagcaaaga caacagcaac taaataaaaa gatacatttt cgacgatatc aatggtagaa
    28501 attagtttaa aattaagctt tctttccaac aggactgaag gaatggtttt gagagaagcc
    28561 atagcgaaag aaaaacaaag agaggtaaaa atccaaaact ctttgggacc ataacctttg
    28621 gttaaactga ttttggagta aacaaagaaa ataacaatta gggcaataaa aactaaaatt
    28681 tgctggatag taaaggtgga ttggagttcc ttttttttga cttcagactt tttttgaatc
    28741 agagcggcag ccaaaccgac atcagaaaag taacctaaaa taccaactga ttcggcaact
    28801 atagcaaaca agccgacttc ggcggtgcca agcaggatag tcaaaagaaa aaagccaaca
    28861 gtagagattg attgaatgcc aatattgcgg agagacaaaa ataagacgtt tttggtggtt
    28921 ttttgtttta tttcttggag attgattttc gccataaggg gtgagaaaga aataaaccaa
    28981 aaaataacca ggttaccaga gaaatgtagt ttgagacagt ccggaccgga gtgttggtaa
    29041 atttgacgaa tatttgatga tagccagcgt caaggttgat aaccagacgg ccaagttcgg
    29101 gttcgatatc ggtggcaatt ggtttgccgt tgtctaagac gacaaatttg gggaaggcta
    29161 actgggacaa aatcacccga gaagattgat ttagattgag attaaacaac atccaatcgc
    29221 tgcctttttt gccgccggtg atggtataaa agacatcttt gggggtgatg tcgtcaataa
    29281 actccttggc ggggccgagg gcggcaatgc gggcggtagt gggcagataa tcgtagatgc
    29341 cggaagtaac ctgatttacc caagccttgc cggaaaattt ttgttcatca gtaataggac
    29401 cgtgttgaat cggagtgaaa taaccaagat tgagcaaaag aactgccaga gataaagcta
    29461 atgagataac gatgattttt ttggggaaat atttgttgat tagcagagtc agaccgccaa
    29521 tagataaaga caaaagaaaa gatgaatggt taagaaagcg ccaagggaat tggatttttt
    29581 ggataatagg aaagagaagc caaataaaag tagacttgtt gtgggtcaaa aatatggtaa
    29641 aaaacgccat aaaaaccaaa agaaggataa gccaaaaacg agaatcaagt ttggatattt
    29701 tggagcggga cttgtaaata aaaataagag aaaaaattac caaaattaag ggaataatcc
    29761 aatggagata tccgaccata aatgacatgt cgtcgttggg accccaaacc gaggctccat
    29821 cgccccaaaa attgctgata aaaagctgat acaaaccggc aaaatggaca gaaaaatgat
    29881 aataaccttc aaacatggta ttaatttgga catatttggt ttccagtaaa actggaatag
    29941 tataaaaggc ggtaagacca gcagcaaata agccggataa aattaattta ataatgagag
    30001 agaactgatt tttaatgaaa gggaagaagg gttttttgta tcggctaaat atccaaaaga
    30061 ttacccaaaa aaacaatatc ggagtaaagg taagagccat gggattgtgg gataaaagaa
    30121 tgccggcaaa agaagctccc aggccaaaaa taaacttatt tttttgttcc aaaacaagtt
    30181 tgcgggcgta gtaaaaagtt aagggaaaaa atactgccgc ccaggcttcg ttcatagcac
    30241 cacggacaaa aatgttgaga gcatgataag gagcataggt ataaaaaatc gaagcaataa
    30301 agccgccggc agggccaaaa attgaagctg ataatagata cataaaggcg gcggctaaaa
    30361 ttatctgggt aacggcggta gctttgacag aattgacgaa agaaaaacca aaggtgcgat
    30421 aaacctgacc aaccaaataa ggcatagggg ggtaaaaatt gaaaagagga tagccatagc
    30481 catagcccaa atcaggagtc caacggcagg gaatttggcc gtcttggaaa catttttcca
    30541 tttctaactg acggatcatc tgcatgtcat cgtgcatatt gtagtagccg ggcttgagaa
    30601 gaaagaaaaa agcaggaatg gtgaacaaga aagtaagaat cggccaaaaa tatttatgct
    30661 tgataagttt agacatagtt tattgtagca gttgagctat ttgagacggt atagcaataa
    30721 tttatcgccg tcaggtttgg caaaactttg aactaaagcc agtttatctt ggtctaaact
    30781 ggacaaagtt aagcgggact gattgattaa taagtaaact tcgtcgccga attgacgggc
    30841 ggtggtcaga cttgagggaa tttgagtaaa gcccaaacct tgaccaatga cggttagttg
    30901 gggaacacca tcggtgtaga tttgtaaacc gtcgggcagg gtgccaaaag aaccttcggt
    30961 gccgacgata acattagctt ctttggccct atttttgagt agatctgcgg ctggtttaat
    31021 cccccagccc gaagtccagt cagaaagata accaacctca gtagaaggaa gtttaacctc
    31081 aaaaggagta agagaaatat ttttgagcca aataatattg ggaataagca gaagcaagat
    31141 aaagataatt ttgggaaaat tgcgactaaa ccaaactgaa agcccaaggg tgataagaac
    31201 cactaacggc ggcagaataa agagaatata tctggcggta aaaacccgag ccatggcaga
    31261 attggccaaa agcggcaaaa acgcccaagc aaggatagct aacagctgag tatttttttg
    31321 gtagaaaaaa taaagaccca gaccaataag acagaaaaac aaaattggcc aggaaatgta
    31381 ttgaccaaag atagttatag cgctgccaaa atgcgaccgg aaaggatcaa aagggtgttt
    31441 gataatctct gataagggcc aaatatagtc ccgatttcgc agagcgatca tatgaaattg
    31501 aggaccaagc cgaagaatat tgtaaattaa aaaagccagt atggcagaaa ttaggggtaa
    31561 ataagttgat ttaagattgt ccagatttag gataaaaaaa gtgccaaaag aaagggcaac
    31621 aaagtaaatc gccggagatt tggtcagcca ggataaacct aaaaccatac ctaagataag
    31681 ggaaagatca agacgtctaa atttggcgag aagcaaagaa aaaaataaag accaaactcc
    31741 aaaagcagac aggagattgt cgggaactgc cagccgatcg aagaaaaaag aaaaaggtag
    31801 aaagatataa attatggctg ataataagcc taaataaaaa ttatttttga gagaattttt
    31861 gataaaattg gccggattga atgattgttc aaaaaaagag ggataaatgg aggcaacaag
    31921 aaaaatacca ataagggtaa aaaaaccggc aacaactgaa accaaacgac cggcaatcag
    31981 aggatcggcg accaaccgca ggctcagcga ggtcagccac ataaacaaag gctgtttgcc
    32041 gtcggtttga ggaacaaagc ggagagtttc ttcgtttttg atgatttgag accagcggat
    32101 atagatagcc tcgtcgccaa aaacagggat agcatcaaga tgttgtagtc gggtgaaaaa
    32161 ataaaccccg gcaacagcca aaaagagaat gaccttaaac caaaaactat tttttttgat
    32221 catataagcc aagcagatcg ttgcgtttga cccgaataat ttcctggagc atttgtttgg
    32281 attctttttt gaatctggtg gcgtcatcgc ctttggtggt gctggcgtct tcgtcttgcc
    32341 actgaactaa aacttctttg attttgtaac cccatttttg ggccataaac aacatttcga
    32401 catcaaaagc agacactcgc caaccggaat tgttggattt ttctttgaag aattgaagtt
    32461 cgggaaagat ttttttggca aggtcagttt tgagagcctt aaaaccacat tgagtatcaa
    32521 taatatcccg aagcaaaaat aaacgccggc caaacaaaaa acctttagcc ataagtttgc
    32581 gaacaaaaga agcaccctga cggacagcgc cgcgggaacc aataactaca ttataaccct
    32641 tgtcaaagta gggcaaaagt ttaagactct ctttgagagg agtggactga tccatatcgg
    32701 taaacaaaac aatgggatat ttagcttctt tgataccgcc ccaaacagcc gagggcttgc
    32761 caccgtgagg taggtcgaga acccgaaaac cggggtggtt ggcaacaaat tttttgacca
    32821 gttttagact attgtcggta gattcgtcgt tgcagaccaa aacttcccat ttaaactttt
    32881 gtttggttaa aaactgatta acttcgtcca agacgcctct tttgagattg ttttcttcgt
    32941 tataacaagg gataactatt gaaattttag tcatataaat gattatagct aattattttt
    33001 tattttttgt aattttacta cctttactcc atctgaagta ataaattcat cgactttgtt
    33061 ttggctttca aatactttta tttcccaaat gtcggaattt ataccgtcag gccatttgtt
    33121 aggatcatca atgacaataa aaagagtgtc agcttcctga tatttttccg gtgacaataa
    33181 attatcagac ttaagaaaat atctaggagc aagggcttta aaatcgttat acgaggctaa
    33241 gagaacaacg ttgtattgac cgtctgaagg gtcaaggtga ttatttatgg aagtagcgat
    33301 gttttgggcg tgcactaatt gattttgagg ttgatttttg aatggattat taattaaaga
    33361 aataattgta attaaaataa acaagggtat accgatgtat ttaaacaggt ggatgagaat
    33421 ggcgatgaga ataaaaacag ccggaaaaag gaaggcaaaa tagtgagcat aaatgtgttg
    33481 tttgtataaa gccagagccg aaagcccaaa aagataccag ccaaaaataa cccaaaaata
    33541 ggtttttttg gtattttttg acaaaaggta aaagccgatt aaaaccgcaa gggcaagact
    33601 aataataatg ccaaaagttt ggttttgggc cgccaagaga tcggtattga cctgattaaa
    33661 aatggcgggt aactcgggaa tagctttgta aggtttgata ctgacggtag tctcccgata
    33721 ggtgaaaaaa ttaatgagag ccttgatatt ttgaccatta tgtttaaggt caaacaaaac
    33781 ctgaggggtg agggataaaa taaagactaa aatggcgatc aaactgttag taaggaaagg
    33841 tttgagctgt ttagatttgg ttttgagatg gcggtagaga taaaaaagcc aataaagacc
    33901 aggaaaaggt aatagtgcca aacccaaata atgactattg ataaccatga caaaacttag
    33961 ggaggcgtaa ataaaatact tatacttttg ttggataatg gcttcaaaaa agaaataaat
    34021 aaaaagcaaa gaaaacaaag gcataacatt ggggttccaa ataaaattgg aatacttgat
    34081 aacgacgggc gaaatagcaa aaagaaaagc ggcgataagt ccaacagggc ggctgaacca
    34141 tttttgacca acaaaataga ctaaaacaat ggtggccagg ctcaaaagag caataaatgc
    34201 cgccggacca acaggattga aattggctaa caacaaagac ggagcaatca ggtaatagaa
    34261 gtaggggcca agatacatat taccaatact ggtttgagga ccaataaaaa agagatcttt
    34321 gtgtttaaaa aagttacgaa taactaaaac atcacgccct tgatctccca aaaattccat
    34381 ataatccccc agacggtaga atcttaggaa agtacctaaa ataaggatta agactaaaag
    34441 ccaattttgg gttagaaatt ttttaatcac gcttgaagtt aattttatac caaagtcgga
    34501 aaaggtcgga aaaacttttt agaattactt tgagattgcg accggtagct tgaccttcgg
    34561 tgcgaggata atgatgcact ccgacttcgg caatcttaaa accagctttt ttggctttga
    34621 ttaaaaattc gctgctgatg aaagctccgc gttcggattc aagtttggga atagtgtcaa
    34681 cgactttttt tctgattaac ttgaaggcac agtcaatatc ggtgacttta agtccaaaaa
    34741 ggataaaaac gatcaactcc caaatttttg aagtaagaat agttaacttg gaaacctggc
    34801 gggacaaata gtagccgata acaatatcgg cttgggtttg ctgctgtttt ttgatgagat
    34861 tgacaacttc gccaaaatcg aattgaccgt cagaatcggt aaaagtaatc catttgtatt
    34921 tggagttata aagaccgctt ttgactgcgc caccgtagcc gcggttggga ttgtgggtga
    34981 taattttgat ttgttgggga aattgctttt tgatggaggt taaaacggcg gcggtattgt
    35041 ctttggaacc gtcgttgact agaattatct cccacttgcc ggttattttt ttgagaacgg
    35101 ggatggtttt agcaacggtg gacttgatat tcttttcttc gttgtaacag gggtaaaaga
    35161 cagaaagctc atcaatcagg tatggtttca taagtagagt atagcaaaat agtgatttta
    35221 gccaactatt aagggctcga cttgacaaag ggatatttta tttatataat gtcctataat
    35281 tatttattaa ataaataaaa tgaagcaggt gaaacaaaaa aatttattgt tagccctggc
    35341 tatggcagct attatggcag gatcgttagt aagccctgct ttggtaaaag ctgattcgta
    35401 taccagtgtt gataatagaa aaacattatc aattgacaag aaaataagat atatattttc
    35461 tgccaattat gttgataata tctccagttc aaccaaggta tttacccaag gagatatcgt
    35521 cgagtttaaa attaaggtga ctaatacggg cacaactaat atgagaaata ttaaagtgac
    35581 cgatcagctg cctccgtttt tgaaattaat tttctttccg ggttcataca acaagaccga
    35641 taataaagtt gaatggacaa ttgatgaact taacgccggt cactctcagg agtttttgat
    35701 cagagctaaa attgatcaag ccactgaagt gaaaactttg actaaagaaa ccaacgtagc
    35761 gatagctaaa gttgatgaaa ttagtgaaaa ggatgacgct gtctattata taggcagtcc
    35821 gtcagctcaa accaaaggcg gtcaaatcat agttccggaa accggaaatg tcggtttgat
    35881 atttcaaacc ggcggagtca tctctttggg attggctgga ttggctttga gaaagaaaat
    35941 ccgcggatac taaagaagac tgtaataaaa gacaaaatcc cctaccgaaa ggcgggggat
    36001 ttttggttat ttggtaatta aacgcaaact cataaccaaa gccggggaat aacggtcgga
    36061 aaatttttgg tcggccccaa accagggatc gtgaatagta tagttgccgt tgctgccatt
    36121 aataataaca ataaagtgca tgccggcaac agtcgccatt tttacctgag caataacata
    36181 gcgaccggct ttgagttcat catcaatcac gccgctatta taagaaactt gtttgtaggt
    36241 ataacctgat ggcggagatg gggctaccat ataggcggta ttaccccaaa aattacttga
    36301 attggcggcg taggcagatg gggtaatatc aaaacctaat tttttgtata ccatagagac
    36361 actattgata aaacagccga cttctccgat agtatcacga gaattgccaa taaactgccg
    36421 acaccaccga ggatcccgct gagaataaaa attaccatca ctgccagcgc cgggagacga
    36481 tcctagacaa gtgctgccgc cggcagaact ggaaaagttt ttgaaagccg ccagctgtga
    36541 ttgagcagca gctaataatt gttgatattt cttttcgtca ttttgagtgg ttttaagcag
    36601 actgtttttg gcagcttttt gactaataag actggatttt tgtgaagata atgtcttttg
    36661 gagattttcc atttcggctt gttttttggc tttggcagtt ttttggttgt catagttgga
    36721 gcggatggtt tccatactaa taagactatc ctgactgctt ttttggacgg aagagatata
    36781 gcggtgctgt tcaagaaaag tattaagatt ggaataaaag agataggcaa aaaccggaat
    36841 tcgtttttgg agtttataat tttgaactgt ttgatgaata taactttcgg aaagtttggt
    36901 aatatcaatc tcaagcttgt ctatctcaac aaccaattga gagatttctg tttccagttt
    36961 tttgatggaa ttttcggttt gggtaatccg aagaagggtc agttgatgtt gagagtcaag
    37021 caatttgatt tggtttgata gggtgtcttt tgacttgccc agctcgtcca gtttttggat
    37081 atattgatca atttgagatt ttaattcttc ttcactttgg gcgcgacaag tttgggtaag
    37141 ggaagcaaaa aaaatgaaac cgacaactaa aataaaaaac tttttgaaca ttattaaaat
    37201 tttatgtatc tcttgacacc gatccagctg gccaacaatc cgatcaaaca cccaaaaatt
    37261 aattcaataa aaattatgag agaaaagaaa ctaaggtctc cggagacaaa atttacaggc
    37321 tggaaaaatg aattgactga atttcgggaa aaatacaagg taataaaaac caagatcgag
    37381 ccaataaaac tacctaaaag tccataaaaa ataccttcaa gcaggaaagg ttttttgaca
    37441 taaaactttg aagcgcccaa aagacggctg attttgattt catctttgcg gttggtggtt
    37501 ttcataccaa taatggaaac aatggaaata aaactaatag caataatgac gatggtgata
    37561 actaaacccc cacggcggat agcattagtc caatcgagga gagattcgat gacatctttt
    37621 tggtagataa tttcatcgac aatatcggtt ttggtggcat aattctggta aatttggtct
    37681 aaggttttgg ggtcggaaac ggttacttcg atagaagcgg gcaaaatact ggcagtgacc
    37741 atttcggtca acatgggatt gtctttgttt tgctgtttgt agatttccaa agccttgtct
    37801 ttggaaataa acttaacttc tttgatatta gggaaattag ttatctcttt tttgagatta
    37861 tcgatagaaa tctgatcgac gccgtctttg ataaagatgg tgatttcggg cttggtttca
    37921 aaatagttta aaatttgaga aagaccggaa ttaacgacaa caaaggcaga aatcagaata
    37981 aaactgaatg tcattaaaaa cagagccgtc aagctttgga taggcgatcg gcggatgtgg
    38041 tgccaggcag ttttagtggg tgtcattttt ggttgaactg gtattttttt tgacaggggc
    38101 ggctttggga gtagtgccgg gagtaaaatc tttgacaatt tgaccatctt tgatttcaat
    38161 ccggcgtcgg tcaagtgagt caacaatttc ctggttgtga gtggtcataa taatagtagt
    38221 tttgtttttt tcgttgatat ttttgagcaa cttgactaaa ttccaggaat tttcgacatc
    38281 aagattgccg gtgggctcgt cggctaaaat aattttgggt tcaatggcca gcgcccgcgc
    38341 caaggctgcc cgctgaagct caccgccgga aagttgggcc ggaaacaaat atcgccgact
    38401 gttaaggtcg accaacttga tgacttcatc gattttggta gaaatttttg accggggata
    38461 tttgataata tccaggctaa gagcaatatt ttcttcgata gatttgtcca taatcaattg
    38521 gtaatcctga aagataactc caatttgacg gcgaataaga tcaatttcgt ttttggtgcc
    38581 gagactgagg tcggtgccgt cgacagttat ctgaccacgg ctgggtttga tttgatttaa
    38641 aaggagtttg atgatggttg atttgccagc gccggaatgt ccgactataa aaacaaactc
    38701 accctggtcg acagtaaagc ttatattact gagagcagtg ataagaccaa aatttttatc
    38761 aacactgtca aaaacaattt tcattttatt cggtgatttt gatactgcca acatccatta
    38821 accaggcagc tttgtttgat ttgttggttt cacctttgac ttcaacctgt ttgccgacaa
    38881 acaagtctaa gtcgacgaca gacgaagtta gggcggcatt ttgagtaact ccgccggggc
    38941 gttctagagt gtgagtcccc tctccgttga cgccgccgga tttgataaca ccaacagcgc
    39001 tgtcggcaaa agtttttgaa agattgccat aaaccacacc aacttttatt tcttcacctt
    39061 cgacaatttt ggcggcttga ttgtcgacta gactagtgtc agcatcgaca gtagaggtgg
    39121 tttggctggg ccagaagcgg gaaatccaaa agccagccac aatagctaat acaacgataa
    39181 tggcataaac atattttgga gtagtggaaa cggttggtgg aggaggagaa actggtttgt
    39241 tgggagatag atttggtata tccataagaa aaattaaaat ctaataattc agtatagcaa
    39301 atcagatagc gtcaaaaaaa tggcgaaatg aagtgtaatc tttatctgag ggaagaaaga
    39361 cccattgtcg gttaaaaaga cgattgggat ggtaaattaa accatcggcg gcggtattgc
    39421 cgataggaat gttaattttg tcaaaagtta gattttttat ttgataatct aaatgagaaa
    39481 taatggcaac tatatcattg tcggaaatat ctttgataag ggagcggttc caaaaatgat
    39541 aaagtttaac cgcctgagaa taattttgga tgaaattccc tgatttaatt ttgtctaaaa
    39601 tagcttcgat aagtagctgt tggcggtgaa tgcgggcaag gtcagtgccg ccttgggcgg
    39661 cagtttgacc gcctttgcgg gagcgaacaa attcggtgac attagttttg tctaaatgaa
    39721 tgggtcctga tttaaattcg acggttttgt aaataggaat tttgggcgaa ggattgttaa
    39781 tatattcagg attaggatat tgatcatcaa caaaaccttg gtcaagaacg agatcaactc
    39841 cgccaataag atcgacaaaa tcaattaagt tgtcagtggt taggactaaa acgtggtcaa
    39901 ttgactgacc agtaagctgt ttaaacttgg ctttgataaa ttcaaaagaa tcgggttcgt
    39961 ttaaagacag ggggtaaatt tggttgattt tggtatttag gtcataaaac caaagatcgc
    40021 gaggaagaga aactagacta attttttggc gggaatagtc tagggaaaca aaaataatgg
    40081 tatcggtagt ttgggttttt tctaactgat cgtcacgctt gtccaaacca aggacaataa
    40141 aattagtttt ttgggattga tggcggtcat aatttagccg ggctgtcgtg acaaggtcaa
    40201 gcagatattg gggtgattga cctaagtcat aagaaatctt tttgaatccc caaaaaacca
    40261 aaccggcaaa taaaagaaaa aaacagacta aaaccaataa agtgtacctg atgatttttg
    40321 acatttataa ttttaattca gcttcgatga aagcgtcaag atcaccatcg agaacagcgg
    40381 cagtatcctg agtttcgtgt cgggtgcgga gatctttgac cattttgtaa gggtgaagaa
    40441 cataggaccg gatttgatga ccccaaccgg cgacaacatt atcaccttta atattttttt
    40501 gctcggcagt tctcttttct tcttcgatag cccaaagttt ggaagttaac atctgcagcg
    40561 ccatttcccg attttgctcc tgacttcgct gactctgaca ggaaacacta atgccggtgg
    40621 gttggtgaac taagcggaca gcggtagaaa ccttgttaac attttgacca ccgtgaccac
    40681 cggaccgaaa ggcagaaaat tcgatttctt cgggagggat tttaaaatcg gaagcaaaat
    40741 ccagcacggg agtaacttcg actttggcaa aagaagtttg ccggagattg tcagcattga
    40801 agggagataa tctgaccaaa cgatgaactc cggattcttt tttgagataa ccgtaggcaa
    40861 atgggtgact gattttaaaa taaactgatt tgatgccggc ttcttcaccg ggaattaggt
    40921 ctaaaacttc atatttccac tgccgacgtt caaaaaaacg caaatacatt cgttgtaaaa
    40981 tagaggccca atccatagct tcggtgccgc cctgaccaga gtgaatagat aaaatggctg
    41041 atttttgatc atattttccg gataaataag tttgaagttc cagagcggaa atatcagtct
    41101 ctaattgttt gatcaaatgg tcaacttccg actctagtga tgggtcggga gtttgggtca
    41161 aaaggtcgct aaattcctca atagttttta tttgggaagc aacagaatta atttgagaaa
    41221 tggttttttg gagatcagaa attgattgga gcagttcttg agctgaggcg ggattgtccc
    41281 aaagattggc ttgttgggat tttttttcca gttcggttag acgctcaaga ttttggacaa
    41341 aatctaattt ttgtttgatg gaatcaaatt tagtttttag ttcgggtaag atgttcataa
    41401 attaattttc gtcgatgttg cgaatcatgt catcagaaat attttttatg aggttttttt
    41461 tgatgtcttt aatttgcttt tggggaaagc cgtccagttg aatctgaata tttttgatag
    41521 tatcggcaaa aaatttgccg attggagttt gattaatatt ttcaatttga gaccggctgg
    41581 attcgggcaa ttgggaataa aactcgccta aaatatcgcc gccaaaagac tcacctaaaa
    41641 cagcgctggg attgtctgtc ggcttggaat tgatggtttt gcctgccaaa aaaccaataa
    41701 gaataaaaac aagaaataaa aagataatca ggcgcaaaat actagaaaaa ccgattttgg
    41761 ttttttcttt ttttttgagc gacatagttt agggcttgag tttggcggag agaaactcaa
    41821 tgatgggagt gtccccttga atacattttt gcccgacagg atcgaaagaa gtgggaactc
    41881 cgataccacc attatcgatt agctcggggc aaaatttgga agctaagccc aaaagttcct
    41941 gttgattgtt cttgtcctga tagacttctt tggaataaat tttgagctga ttgtctttgt
    42001 tattatcagt cagccatttg tcgacatttt tgcagtgagg gcagccgtca ccccaataaa
    42061 acactaaatc gtaattgtta aaatcagcgg gaacttcaaa attagcaatg tttagagcgt
    42121 tgtttttggg cggctgagac aagagaaaaa ataacccaac caaaactgct aatccaataa
    42181 agatataaat tagtttattc ataattattt tgtttttgag gttaaactga taattttgtc
    42241 gccctgatta atttgatcta aaatatccag accggaaacg acttcgccaa agttgacgta
    42301 ttctgaagtg agatgttgac aaccagcagt ggtaaaacaa ataaaaaact gactgtcgtt
    42361 gctgataagc ttggtttcgg cggtgcgagc caaaccaaga ctgcccctga caaaaggagc
    42421 gtcatttagt tctgaatttt gtttgccacc acccataccg gtgccgtcag gatcgccgcc
    42481 ttgagccata aaatctttaa taacccgatg aaaagtcaga ccatcataga agccactgtc
    42541 agctttttta agaaaattag ccacagtttg gggacattta tcctgatata gcttgatgag
    42601 aatttcaccg tttttggttt tgagagaaac cacaggttga gactgaggag tggcggcggg
    42661 agaaacagta acattggcgc cagtgggaag attgtttagt tccatattgg tagggagagg
    42721 ttttttggaa gtgcatccag aaattaataa tgaagagaca acaactagga tgaaaagttt
    42781 taagttttgt ttcatatggt ttattttaac aaaattaaga cgcgcgggct tccttgattg
    42841 atatcctgtt tgacaccgga ggtagggaaa tcgtgaatgc ttttgagcaa cagattgtaa
    42901 ctatacttga aataagcgcc aaattgggta ccgacatcgt gaacagtaaa cttgctggtg
    42961 ttgtcatcgt aaccaagaat aactaaatta tgatagtagg ggccgccaga tttgaaatgt
    43021 ttattttcgg catagagagt tttgccgttg gcaggaacaa taatggggat atctttggca
    43081 atataagatt tgagatcttc aagagaaggg ttgtcgatga ttttggcggc ataattgagg
    43141 tgattttggg caattaaaac catttgggac agatcaacat catgagtaaa attttgatca
    43201 gcttcgtatt tgatcatagc caaaatatcg tttttaatat caagactggc aggagtaata
    43261 tttttgtaaa aataattaac agtcagaagg gcagcctctt cgcaggcgtc ctgccagggt
    43321 tgatcccagt ttttttccgg cgattggggg ataaaagcgg ttttgataag atgtttgttg
    43381 ggcagaccgg agtctaaaat aatggtagga accggagcaa cagtttcttt aaggtcgtta
    43441 attttaacga caacagtgtc aattttttga tcaatttttt ggggataggc tatttttagg
    43501 gaaaagtaaa tcaaaaccgg caagctgata aagataagaa aataccaaaa tttattggac
    43561 atagataatt atacaaaatt aaccatagaa tactacaatt ttgtgattta tccattgaat
    43621 aattaaaagg ttgtagtaga ataagcgaat gcccaaacta acggatatta gaaacatagc
    43681 ggtgatagcc catgtggacc atggtaagac aactttggta gacgctatgc tgaaacagag
    43741 ccatatgttt aggagcaatc aggatgagat gaaaatggat cggattatgg acagcaacga
    43801 tttggaaaga gaaaaaggaa tcacaatttt gtcaaaaaat acatctattt tttttggtga
    43861 aacaaaaatc aatattatcg acactccggg acatgctgat tttggcggtg aagtagaaag
    43921 aattttgaat atggctgacg gagctctact gatagtggat gccgctgaag ggccgttatc
    43981 acaaactaag tttgtgctca aaaaagctct ggaatacggc ttaaaaataa ttttagtaat
    44041 caataaaatt gatagaaaag accagcgaat tgccgaagtg ataaccgata cggaaaattt
    44101 gtttttgtca ttggtggatg atttggaaat gttggatttt ccgattattt atagtatcgg
    44161 caaggatgga aaggcctaca gccagctgcc ggctaatttt gaacaaaaag ctgatattaa
    44221 tcccctattg gaacaaatta taaaaacggt gccaaatgcg gttcaggaaa tagacaaacc
    44281 atttcaaatg ttgatttcgg cgtttgaaaa aaacgaatat ttgggtaaat tgtcggtggg
    44341 aagaattcat accggagtaa ttaaaaaagg aatgcaggtg tcgctggtgg acccagacca
    44401 aaaaaccaga ggaaatttta ggatagaaag aatgtatgtt aatgaaggta tcaaaaaagt
    44461 ggaagtggat gaggctgaca gcggcgaaat agtttacctg gcaggagtgt cgaatattga
    44521 aattggacaa actttaactg atgtgaatca tcaggtggcg ctgccgacaa tcgaaattac
    44581 tccgccgaca attaaggcta gtttcggtcc aaatacgggc gtatttgcta aagatgaagc
    44641 aaaatttctg accagccggg agttaaaaga aagactaaaa caggaagtgg aaacaaattt
    44701 gggattgaaa ctggaggatg atccggtaga cagtatcaga atgctggtat caggcagagg
    44761 tgaacttcat ttggcgatat tgattgaaga actaagacgg cagggatatg ccatggaaat
    44821 aggcaaaccg gaagtaattt tgaagacgat tgacggagtg gtttgcgaac catttagtga
    44881 gctgacaata ttaactccgg aggattttgt ggggacgatt acaacagaaa tgggcaaaag
    44941 aaaggcagaa atgacagata tgcagactga cgataaaatc ggggtcaaaa tggtgtataa
    45001 aatcagcgaa aaaaatgccc tgggattaag aagtattttg atgacagaaa ccaaaggaat
    45061 ggtgtcaatg tcatctttgt ttttgggtta tgaaccaaaa agagacgaag aaaaaaaaga
    45121 aagaaatggg gtgctgattg cgtcagaatc aggcaaggct ttgtcttatg gtttggattt
    45181 ggctcaaaaa agaggaatca cttttgtgga accgacagaa atggtctatg aaggtcagat
    45241 agtgggcttg agaccgctga tgggagattt ggaaataaat gtctgcaagg gaaaacagct
    45301 gtctaatatg agagctgccg gtagcgatga ttcgattatt ttggcaccgg caaccaaata
    45361 ttctttggaa gaatgtttgg attttattaa cagtgatgaa ctgattgatg taactccaaa
    45421 aaagattagg ttaagaaaga agttgttaag caagattgac cgggtcaggg cagaaagaaa
    45481 cactagataa aattaaggtg aaggtttggg aaaaagagag ggtaaaaata attccagact
    45541 attgtttgat ttgggtggat tttggaaaaa accgactaaa tttttaaaaa acggttgttg
    45601 gagcagaaaa taagacaaaa ttgaaactaa aattagagtt cccagtagcg agccaaggga
    45661 agcaaaaaag ccgttagtaa atgaccggcc aatacgccgc caaggttcat tttggagctt
    45721 gatctggtag gtcagttttt tgatttcgtc aattagttcc tgatttttgt cgatttggtt
    45781 tggggttgga ggagtggagg taatatcagt cataattaat tatagtttaa ttttattttt
    45841 aatttcgacc agctgtttgt catctagctt ttggtgagga aatggttgta agaccggttg
    45901 aatttggtcg cctaggtttc ggggaataat gtgaatgtgg gcatggggga catcgttgcc
    45961 catagtaaga aaactaataa actccggttt gagagatgaa tttttgatat tgagagcaat
    46021 tttttgggct acttgccaat attgatcaaa attgggcacg tcataaaccc agcgataatg
    46081 gtgtttggga atgaccagag tgtggccgac agctacagga ttaatatcca aaaaggctaa
    46141 aaaatctttg tcctcataga tttgatggca aggaaggctg ccgctagcaa tacggcaaaa
    46201 aatacaatcg tccataatta gattttatca attttggggt gaagtttttt gatgatttgc
    46261 aggactaaag ttttggcttc ggataagggt tggttgatgg tggcgccggc agccgctttg
    46321 tgaccgccac cggctttggt ggcgacagcg atttgagata aatcgtaagt tcggctatcg
    46381 cgggtgcgga aactgacttt gaccgtttgg ggttgaattt caacaaaaac tagagcaata
    46441 tcccaaccga taactgactt gatcatatta gcgacatcag aatagctggt attaaccaca
    46501 gattctaact gatgtttttt gatatcttca taggaaagag aggcgatggc gacatgacca
    46561 gaataataaa tttcgattga gcctaaaagt aaagaaataa gttttaacct ttcgggagga
    46621 tcattatttt cgatatcaaa aatcaattgg gaaaatttgg gatagatttt ggcaagttgg
    46681 gaggcgataa ccaaggtttt gtgagtggtg cccgcatatt tgaaaccgcc ggtgtcagtg
    46741 taaatgccgg catagagaca agcggcaatt ttgggagtga ttttgatttt tctgacagaa
    46801 aataaatcaa ataaaacctg ggcggtggcg gggctatgag ggacaacgag attgattttg
    46861 gcaaattttt cgttgctagg gtggtggtca atcacaatag ttttgagagt tctgggaaat
    46921 ttgaaatcac ctaatctggt aatttgcctg agggaagaag aatctaaaat aataaataag
    46981 tcaaactgat ttagatcaat ttgaaaaatg ttttggggta aaattttgtc ggcgccggga
    47041 atggtagaca gattaagagg aagattatta tcgccttgaa taagggtgac gtctttgcca
    47101 agatttttga gaacatggta aaaagccaga gctgagccga cagagtcacc gtcagaacca
    47161 gggtgaagat ggagtaaaat tttttgggag cggtcaattt cccgccagat ttttttggaa
    47221 gtaaatttga aactaagcat aattaaccaa atttaggata acaacatttt ttgtattttt
    47281 taccggaacc acagggacaa ggatcgttgc ggccaacagt tttgtccttt tttatgggtt
    47341 ggggtaaggg tgaaagagaa tcagcgtcag gcaaggattg gtcgggagac ggggcttgag
    47401 gattatcagt cagagagtct cccctacctt cggtaacatt ttgaggagag gtggcttggg
    47461 gggccaccgg ttcaagacgg aaaatttttc tggctaaatt gtattcgaat tgatttaata
    47521 accgttgaaa catttcgtaa ccttctttgc gatattcgac caaaggatct ttttgagcat
    47581 aaccacgaag tctgacaccg tcacgaagat cgtctagggc ggtaagatgt tctacccaca
    47641 aaggatcaag agtggagatg agagaaaaag ctaaaatact gtcggcaatt tctttgccaa
    47701 acatttttac ccgggactgc cactggtctt cgagaatact agttaaaaat tcggtaggat
    47761 tatctgattt ggctaaatct tttttgagtt gactgcgacc ggaagcatta agaggcaaaa
    47821 tctcgttaaa ttcaagcgaa gttttttcca gatcaagacg atggctgtcg tagtcagtat
    47881 taagattaac aataccagta acttgattgg ttaaatattt taaaattagc tggtggtttt
    47941 tgtcgggagt ttctttttgg tttaacaaaa cttcgtcgcg gagacggtag ataatctgcc
    48001 gttgtttgtt gataacatcg tcatattcaa ccaaattttt gcgggcatca aaatggaaac
    48061 cttcgacctt gacctggatc tgttcaatta cccgggaaac taaagggtga gtcagaggag
    48121 tatcttcggg catattgaat cgacccataa gggagctaat tttttcgccg ccaaagatac
    48181 gcataatatc atcatcaagg gcaacataaa actgagaaaa gccgggatca ccctgacggc
    48241 cggcccgacc ccggtctcga atccaaatgc gacattgcct tccacggtct cttcgacgta
    48301 gttgtacccg atcatcttcc ggagctgatc gtctgtaaac tgcgacccga ccgcgtcatc
    48361 atgagccacg gcatctactg cacctggggc ccggcccgag aggccctgct ggccgccaac
    48421 atccccgtca tcacctacgg cgagggcaag aaacgcgaca ccgaaaagtt caactggaac
    48481 acctccgccg actggtggga cgtgtcagcc gagtgggaca aggtccgcga cgtgcccctc
    48541 acgcccgccc agcagaagac catcgacgac tatctggcc
//
LOCUS       DAOSHV010000002        22774 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_1809_length_22774_cov_6.260663, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000002 DAOSHV010000000
VERSION     DAOSHV010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 22774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22774
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1809_length_22774_cov_6.260663"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="PKZ29_00240"
     CDS             complement(<1..304)
                     /locus_tag="PKZ29_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37331.1"
                     /translation="MIHLYLGLLLFSFLITSILVIPFINILYKLKFQRQKQQTTDFLN
                     KKTPIFDKFHNSKTGTPVGGGVLVIIAVTVLFFVLFPVIKFSKIFISSNYKIASEIS"
     gene            complement(308..1636)
                     /gene="murA"
                     /locus_tag="PKZ29_00245"
     CDS             complement(308..1636)
                     /gene="murA"
                     /locus_tag="PKZ29_00245"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF006873.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOG37332.1"
                     /translation="MSRYIVTGGKELNGEVSIRGAKNASYKQIIASILSPQTTQLTNI
                     PKISDIKITESIAKSIGSTINYCGEHCLEITTPKITNSTVPAGTGEKSRTSFMFSAPL
                     LVRSGKAVVPTPGGDKLGARPLDRLFDCFDKMGIKTEVTNTNMTFNTDKIKAVHYTFN
                     KPSHTVTETVIMAAAFAHGETILDNSAQEPEIDDLISMLNDMGANIHRDLKDPKRIII
                     NGVRQLKGTNHQVIADRNEIVTFACAALATKGSINILRSNPEIVQTFLNTIETMGAKI
                     IPGRDEITVSWVKPLKNIDIETGPEPEFMTDWQAIFSILLTQTVGCSTIIERVYPSRF
                     QHIENLKKMGVKANFFNPKVDNPETYYQFNPESDKPEYFHGVKIYGPTKLKPANFVIN
                     DLRAGASTTLAALTAEGTSIIDGVEYIERGYEKLAERLCSLGANIKYIKT"
     gene            complement(1639..3036)
                     /locus_tag="PKZ29_00250"
     CDS             complement(1639..3036)
                     /locus_tag="PKZ29_00250"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HOG37333.1"
                     /translation="MTNPSLSNSHIHILGIAGTMTASLAVALKQAGARVTGSDQPKIF
                     PPISTTLKKADIKINITDIDHDINQAIIGSSYNLFENTKEEFKQIKQQKIPYLSATKY
                     IAQNIIKPNSIIVAGSFGKTTITSLLAWIFHHTKLKPSYMFGGSSKNKFSPVKITDSN
                     WSIVEGDESIHGLDKTAKFLYYPAKYLIITSADWEHKDSYPDAASNLKTFKKLVQKLP
                     SDGVFLVNQQSPSALSLAQYSPAKVISYNTPESDYFLEKTIPQKNFTTLIIRTPSGII
                     KTNTTLIGQFNFENILAAVALSDYLAISPKIIAKSIFSYRGIRRRLELVAKPKNILFF
                     DDFAQSGNRIKSTLTALNSHYPQKKIKVLYQAHASFSQYKSSLDELKNAFNLADEIVL
                     TQLKFNHNINKKNRNTAKDFRDCLGSKLIYLPLKTQIMQYYKHSLTSNDILIYMSSGG
                     LEGNRIFKSVINLFK"
     gene            complement(3014..3622)
                     /locus_tag="PKZ29_00255"
     CDS             complement(3014..3622)
                     /locus_tag="PKZ29_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37334.1"
                     /translation="MPQYNLNNNQSPEINYSQGLLPQRNQTNFWYSDTSCRSNLKQFK
                     LSSENRRILNKTAGFSYQKIPLKNFKLTSDITKQIFSWIKQLGWDFPTSSVKTVFNKH
                     IFNYLYIWKDEQNNVIAYSICYFSKDISHIAYVFYHPDYSHGNLPIRLVLQTIIDSYD
                     LKLDYCYLGRFSPQNGYYKRNMPGFEYFHHHQWTKYDKSISI"
     gene            complement(3609..4913)
                     /locus_tag="PKZ29_00260"
     CDS             complement(3609..4913)
                     /locus_tag="PKZ29_00260"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008765 -
                     UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOG37335.1"
                     /translation="MWQKIKNRLFHLPKSIFYRHYYHQPSKKLVLVGVTGTDGKTTTS
                     ILLYEILKKSGINAGVITTIGAKYGNEEIDTGLHMTSPDPSVIQKIFKRMVDAGVTHV
                     VCEITAHALDQFRYYGCNFEVSVITNTSHEHLDYFRDMQHYIETKSLIFSHSKISILN
                     RDDASYQIIKHNLGNKPYLTYGIEHKSDYQAKKINLDKNSLTFNLNSLKIKTDSLYYY
                     QIYNILAAFAIIDQLKINPEYALEVVKNFPNTKGRREEVVNQLDILALIDFAHTPNAI
                     KNTLSSLHQVRQGRILTIFGATGGRDQTKRPIMGSVVSQLADIAFVTADDTRNEDIDK
                     INQQIISGISRNSVLIDDKQPLSEIKKIIKNNKDNFVYFNIPNRQDAFNLAISLAQAK
                     DTVIAMGKGHETTILDGNTEYPWSEAEAFRTAFKLRQADASI"
     gene            complement(4928..6577)
                     /locus_tag="PKZ29_00265"
     CDS             complement(4928..6577)
                     /locus_tag="PKZ29_00265"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOG37336.1"
                     /translation="MTQRYRILLFSFILCFLAVTGKLFYWQVVKSPELKEKALSQTYK
                     LETKYPARGQILSSDNFPLALNQTNYRLSLYKPNMTENLDDILNKIDLVYPEFTKQNP
                     NLNNFKSNSNQKWVDLSTNFTEPQKNQLQEINGLIFKKYSSRYYPENSLASNMLGFVA
                     NDNYGYQTGYGGLEAYYNKQLSGRTGFTWSPQDATGKTILTKKSWSSPSIDGQTILTS
                     INRNFQHQAEIELSQGIEQYQADSGSIIVMETKTGQIVAMTSQTASTVATPSASRNPA
                     ISDLFEPGSIFKPIVVTMALDQKSIQPDYICHNCSQPLTIGQYTINNWDKSTHPESTL
                     KDIIKNSDNIGMSNIIRQLGLNNFLKYYQSLGLNQKTGIDLQGEVKPTIKNNWPEIDL
                     ATASFGQGIVVTEINMLQAFNTIANDGVLVNPKVATSLSDQIGKVYPIKSKKETRVFS
                     HDSVLEIKSILKYAVENGAVSVFKPKDLEVCAKSGTAQVAVKGGYTDSATIGSYIGFW
                     PCYQPKFTMIVTINNPKSSSWGSNTAAPIWFKMASKINGLL"
     gene            complement(6577..6831)
                     /locus_tag="PKZ29_00270"
     CDS             complement(6577..6831)
                     /locus_tag="PKZ29_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37337.1"
                     /translation="MSKIFSGIIILSLIVQIVFSFFYSSSIVTQNNQLDLYQKEADQL
                     QLEVESAWKQVADLTSITHLVQSTPSASLKPIIKSININF"
     gene            complement(6831..7706)
                     /gene="rsmH"
                     /locus_tag="PKZ29_00275"
     CDS             complement(6831..7706)
                     /gene="rsmH"
                     /locus_tag="PKZ29_00275"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOG37338.1"
                     /translation="MNFHTPVLLKEAVDTLCVKPHHIYVDATLGHGGHTLEILKRGGI
                     VYGFDLDPNNLKIAADRIKDNNLSPNFFPINQNFNQLNTVVKQKIKSKVDGLIADLGL
                     SQNQQTGINRGFSFNDSQSLDMRLNPDTQTLTAEEIINTYSFDQLFQIFSKLGQELYS
                     KPIALKIIKQRQKSAIKDGLRLADIIRDYYRDRHIKTKIDPATKIFLSLRIAVNQEDK
                     NLTQLLSQSLEVVKPKGTVVIITFHSGEDRLVKQFINRNKHLVQKQKFIKPSLSEIKS
                     NPLSRSSILRSYKIV"
     gene            complement(7706..8404)
                     /locus_tag="PKZ29_00280"
     CDS             complement(7706..8404)
                     /locus_tag="PKZ29_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37339.1"
                     /translation="MNKFKTISFTLIIFLSSLLLSGCGKKAPKTPAETKNSAPKELTL
                     TDSDKPIISLIPRADGHELKLKVDKIPASISQVEYELIYSAKDNGLEMEKGVGDTVKL
                     TSNNFEKDLLLGTASCTNGCKYKFDEDVVGGVLSLTFYTNDNQTASFETPFTLTTSAD
                     IKKNSGLSLSLENLSITATTTTKNDFFILIKNYRPYYSVFSSGSGTAKITKISPETVT
                     KENTNTLVGDYLIN"
     gene            complement(8406..9725)
                     /locus_tag="PKZ29_00285"
     CDS             complement(8406..9725)
                     /locus_tag="PKZ29_00285"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HOG37340.1"
                     /translation="MVTNQSDLVSKIVSLCKRRGIIFQNSEIYGGVQGFYDYGPIGSQ
                     MKLNWKNLWWQENVVRRRDIVGIDGSIITHPTVWQASGHVKNFADTMVECKKCHKRFR
                     PDLLDDPSKCPDCGGQFTQGRKYNILFETEIGVIEGEKQHAYLRGEACQTIYLDFKNI
                     VDSTRIKIPFGIAQIGKAFRNEITPKNFLYRTREFEQWDIQYFVHPKEMDKWYEYWKE
                     TRMAWYQTYFNNSKNLRFRQHQKDELAFYAKKAFDIEYQAPWGWAEMEGIHWRSDYDL
                     GQHAKFSGQDLSYTDPQTQEKYIPWITETSGGVDRSFFFLLLDAYQEETTSTGDIRTY
                     LKINPKIAAYKMAILPLAANKPELITKAETIFNQLNQKYAVDWDDSSSIGKKYRRQDE
                     IGTPWCLVVDYQTLEDNTVSIRDRDTMEQIRLKSDEIDTWLEKRLNN"
     gene            complement(9906..11705)
                     /locus_tag="PKZ29_00290"
     CDS             complement(9906..11705)
                     /locus_tag="PKZ29_00290"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOG37341.1"
                     /translation="MKNNIIKTLILIFISLTFYYSRQIFFYKYEPEYYENWYYHSQWN
                     IPNSVRGIGDGDLYKFVGYRLAFGENPFNINYEVPPFAKLLYGLAERFFSNPYIISII
                     FYLSSFIILHLISKIFFQKNTHTILLTLLLMATSPFISTQVRETMLDLPLMFFFLVHL
                     LFFLKYLQLDNLQKLIFAGIFLGIATGCKIGVYSPLIFLIGLIFILTTKTKKIKKILL
                     YAFSTLGGYGFSFISYFIYHPNPIPWLRLHEKPIRFYIGQGNSHIDYFNQLKGILINR
                     YQGFWAGAQPGTLGDWSPMLTAGLLLLIVLLITSIKNKNKLTLYLCLFSLSFILINCF
                     IPFFPRYLMPIIPTFCLIITICLQQKPFLIYLLIILNIPFYYTSVIKQPYQGTIESTT
                     RFINTRNYRELYRSISLHQRNLMNEDYFINSYENFLNKIGTRSIESNITSIKFDQDIV
                     TGVLNTTYQTRYGPLENKSNIIFQKINNQWKLIWDWNYLFNRYKPEYQINIVPKLNQI
                     AAIEAFMAPRSMSDWGLNLTQIQDITKIPQQTINDRLRFSVPDKYYRWVGNVNIKNFD
                     ETRKIPGLEFFHYPQKAEISFIDNQNQKIYLFE"
     gene            complement(11748..12308)
                     /gene="recO"
                     /locus_tag="PKZ29_00295"
     CDS             complement(11748..12308)
                     /gene="recO"
                     /locus_tag="PKZ29_00295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOG37342.1"
                     /translation="MDHYKSTLGIVINKKTTKESDLLITLLTPKMGKIVAIAKGAKNI
                     KSSRLGNLQLGNTIKAQLYQKGDFTWISESQNISPFLQTDKNLAQLNLLFYFLEIVNL
                     FVAENQHIDKIFEISQNIIAAINKNLIRQYIQNEIYFLETLGFGVPLDIKTYFSQKDY
                     KTSQKLIKRFFESIIEKPLQSNKLFR"
     gene            complement(12309..14234)
                     /locus_tag="PKZ29_00300"
     CDS             complement(12309..14234)
                     /locus_tag="PKZ29_00300"
                     /inference="COORDINATES: protein motif:HMM:NF024594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4012 domain-containing protein"
                     /protein_id="HOG37343.1"
                     /translation="MDLITKTANTKPGKKVKLGNRRWPKIIAIIFAFIFILALGLFFV
                     VYLPAKELQTKIEAAKTEVDHLKQSINEKDLEKIKINLSVIKDHTKSIEDTYFKLKPI
                     GFLPIINNYYQDGLRTISIAKNSLDTSDIIIKAIEPYKDFLGMTGSAASSEETTQDRI
                     AFLTQSVEGLIPYLDTIEAKIAEIDKSAGEINPSRYPEEFRGYQIKSNITKAQELLSQ
                     SHKLIKDGKPLLSKVSWLLGKDSPRKYLMIFQNDGELRPSGGFWTAYSTMTVDNGKVK
                     PGTASNIYDLDDRINSRTPAPRLIKSYHINVPYLNLRDSNLSPDFPTDAKIFLDQYLK
                     ATGSKEKFDAIIAIDTQLLVDMVAVLGKLDTRVGTFTTEPDKRCYGCPQIIYEMQWMS
                     GRPRDYIEKNRKDFMNPLMSALLANVMGSEKNKMGQLIQAGFKSINEKHVLFYFPNED
                     NQKMGDLANITGKIYDTESSVDYLHLNDTNFASAKSNIFITQKIKHDITIKNGQAQHK
                     INITYTNSAPGSNCNLEKGGLCLNASTYRDIFRFYTPKGSKLTKMTGSEVETVQYEEL
                     NKQVFEGFYGNKFGLNPQSNLKTSIEYTSGAQIGSVYTLMLQKQPGTKPVDYELIING
                     SKQNFSWVADKTIRFTL"
     gene            complement(14263..14904)
                     /locus_tag="PKZ29_00305"
     CDS             complement(14263..14904)
                     /locus_tag="PKZ29_00305"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOG37344.1"
                     /translation="MTDKLYFNVFKRILDIISSVFLMIIFSPIFLLTSILVKITSPGP
                     IFFKQKRVTKGNKEFWMYKFRSMYTGDNDKRLRENYPELWKKYKDGGWKLPMSEDPRI
                     TPIGKIIRASSVDEFPQLVNVLKGEMSLVGPRAYREGELQEYEKKYPTTKKYIDDIRS
                     VKPGITGLWQTSGRNELNFEQRAKLDSKYIRNQSIRQELLIILKTPMSMISRW"
     gene            complement(14901..15989)
                     /locus_tag="PKZ29_00310"
     CDS             complement(14901..15989)
                     /locus_tag="PKZ29_00310"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOG37345.1"
                     /translation="MAKSNQYVVILCGGSGPRLWPLSHAGNPKQFLKILGKKTLLEQT
                     FSRCQKFCPGKNIFIVSNQKYQAKIDKILGKKLPPQNFIYEPEKKNTTMAILLALAHI
                     QKLNPDAVVTTTPADHYIKKIISFRKDIANSQKLAQNHHRIITIGIKPAFANTSYGYI
                     LPRQKNQPLSNVSLFVEKPDKDTAQNLIKKGALWNSGIYTFTLTDIVNEVEKLQPEYF
                     RLYQQLVNSQLDKKTIAKVYAKSPDLAIDRAVSEKSDQIYVIPASFDWSDIGEWKSIH
                     QRSDKDSDNNAVLNSQTNYLAHNSTNCLINGSKNKLIGLVGVSNLAVIDTDDGLLICH
                     LDDSYHVRDLIGKMVKNKKYKSFFLKKP"
     gene            complement(15965..17062)
                     /locus_tag="PKZ29_00315"
     CDS             complement(15965..17062)
                     /locus_tag="PKZ29_00315"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOG37346.1"
                     /translation="MTAKPRVAVFYDWLNQWGGAEKVLLDILQIFPQATVFTLNYHPK
                     KTSWLPSSIKVIPSFINHLPLSHQNPIIYTPLYPLALEKFDFSQFDIVISTTSTVGHS
                     LLTPPSTLFVCYFHNVNRYLYQTPKQFKFLKPILKHYQKTDFIYGQRPDYCLCNSQTV
                     RRRIKSAYNRPAKVIYPGVDTSFFIPKTNPTVSPYFLVVSRLVEHKKIDLAIRACHQL
                     NQKLIIVGQGRDYYRLTKLAAQNSNSQVIFAGKVSPKKLLSLYQNCQALICPQIEDFG
                     LTPLEAQACGKPVIAFNQGGLTETVINHQTGVFFDHQTADSLAQTLAKFDSKNFSPAD
                     CVQNAAKFSRKKFMLNFKQTINQLWLNQTSM"
     gene            complement(17052..18179)
                     /locus_tag="PKZ29_00320"
     CDS             complement(17052..18179)
                     /locus_tag="PKZ29_00320"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOG37347.1"
                     /translation="MTKKHIVIDARLYGPKHTGIGRYTKNVLLSLTHQPDFSHYRFSL
                     LVYPELLSDIKTDLGNSFNYIPTNIRHYSFKEQLLLPLVISKLKPDLVHFTHFNKPLL
                     YFGRSVVTIHDLIKHFFRGKANTTKNSLLYWPKYFFYILITHITIKTNTIIVPSDFWR
                     QYIIKHFHLSPAKITTTHEAVDPDFKKNLSQVNPKSKPVGDYLLYTGNLYPHKNIKVI
                     IQALRSLPKLKLKIISARSFFTHQVEAEAKQNQVSNQIEFLGYIPDSQFKRIYSQAIA
                     LVHPSFMEGFSLTGLEAMALGCPVISSNSSCLPEIYQDSVLYFDPYKPSQLVKQITRL
                     LKTPNLRRQLIQKGCLQINKYSWDNTAKKTLSVYQKTLNDR"
     gene            complement(18176..19099)
                     /locus_tag="PKZ29_00325"
     CDS             complement(18176..19099)
                     /locus_tag="PKZ29_00325"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOG37348.1"
                     /translation="MSKRIDLSIIILNYNSADYLRRCLKSISRSNLGNYAIETIVVDN
                     ASTDLSLKLAQKDKHKWVKYLVLKKNLGFAAGNNQGVKIINPNTKYVLFLNPDTIVQP
                     KTLKTMIDFFNRQPDASAATCYIKLAKTGQMQPECHRGFPTPWRSFCYFSGISKLFPK
                     SKFFNGYFLGHLDTSTIHPIEACVGAFLMVKKSVGQNINWWNESYFFYGEDLDFCYQL
                     YQKKYKLYFNPHCQITHFQGISSGIKSQSQKLSSANRSTKILAAKSSTEAMKIFYQRN
                     LFSKYNFFTRQLVLGGIKLLEIKRLFKAKYL"
     gene            complement(19083..20309)
                     /locus_tag="PKZ29_00330"
     CDS             complement(19083..20309)
                     /locus_tag="PKZ29_00330"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOG37349.1"
                     /translation="MTQKNKRNILMITPYIPRLSQSGGQNSSFYSIKYLSPDNNITLI
                     CLSPDEEGLEDIKQYCSKVIVVKRGKTWDPKKVLLTGLSKYPFLVMKHINKNFRQAIK
                     HELDNNKFDLIHCDCFYPMPNIPKTKIPIVLVDLTVEYAVYQHYVETLSGWKKIFKPI
                     FWIDVLKLKYWETHYWKNTHTVVAFAQEDRELISQVTGRNDIELFQNGVDNKFFESKP
                     KTPRSKFPSVLFGVSNMKWMQNRESVEMIIKDSWPAIKQVIPDCKLYIIGRHAPDYYG
                     HYQSPDVIVQEADFDGQPQDPMYFYQYCWILLAPMGSGGGTRNKFLEAMACRLPIVTT
                     PEGMGGIKIENFKESIVCDYDQVPKNAINLLKNHHQRQAMGEAANKLIAKKYSYQNSV
                     DGLNKIYQKITNVKKN"
     gene            complement(20293..21696)
                     /locus_tag="PKZ29_00335"
     CDS             complement(20293..21696)
                     /locus_tag="PKZ29_00335"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOG37350.1"
                     /translation="MFKNINPIKSIIAILIVFIPLYPKFPLFNVPNTYVAVRLDDIIV
                     ALAVLTWFIYQVTNGFSFLKLKITKLFFLYFLAIFVSFVHAYLIYQTEPTNILLLHLF
                     RRFEYMSLFFITLDAVKSISDFKFIYYFFLIATGLVTFYGYGQKYLHFPVVSTMNSEF
                     SKGQLLQMNIWTRISSTFAGHYDLAAFLSVALIIILAIFLLSQNKFTKIVSLLVWLPS
                     YYILTLTASRVSIFAFLGGVCLTLIFIKKYFWIIPVTGLVIFSIFNSGDLNQRLIATI
                     PALKNQFKSSNNFAPAPTIIPTPTISTPKLTPIPGQIIKPTPTVVRHQPETYPPVDAD
                     VGVARSGEIRFNAEWPRAITAWRRNPLVGSGLGSITLATDNDYLRCLGESGLLGLITF
                     SALIFYFFIKTIPLIFKKSKNTYDMISLICFGGLITTLANAVFIDVFEASKTAYLFWI
                     MLGCYYQSLNLKNDSKK"
     gene            complement(21681..22610)
                     /locus_tag="PKZ29_00340"
     CDS             complement(21681..22610)
                     /locus_tag="PKZ29_00340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOG37351.1"
                     /translation="MINPILSIIIVSYNTADITLNCLKSIIQDKGLNQIDYEIIIIDN
                     NSQDDSLTKINRFKINLKSKNISLKLIANKTNHGFGKANNQGLKIASGNYILFLNSDT
                     LILHSAISQSLDWLCSHPESSTCTAQLLNQDKTIQASGGFFPNLGNVFTWCLNLDDLP
                     LVNKIIRPIHPHTPEFYTKDRFYLSDHRQDWVTGAFFLTRKPLLDKVGGFDENYFMYG
                     EELELSYRISQTTPNNHVWYLIGPQIIHLGRASAAKKLDPILNEYKGFLSFFQKHKPK
                     WQYPLVKFLLKINALIRSVIHFSPVYLQACSKI"
BASE COUNT         6671 a         3349 c         5504 g         7250 t
ORIGIN      
        1 tagaaatttc actggcaatt ttgtaatttg aggaaataaa tattttggaa aatttgatga
       61 caggaaatag aacaaaaaat aagacagtaa cggcaataat aactaagact cctccaccga
      121 ccggggtgcc ggttttgcta ttgtgaaatt tatcaaaaat aggagttttt ttgttgagaa
      181 aatcagtggt ttgttgtttt tgccgttgaa attttagctt gtagagaata ttgataaagg
      241 gaatgaccag aatactggta attaaaaagg aaaataatag taggccaaga tacaaatgaa
      301 tcataattta ggtttttata tattttatgt tagcacctaa cgagcaaagt ctttcggcta
      361 acttttcata acctctttca atatattcaa ccccgtcgat aatggaggtg ccttcggcgg
      421 tgagagccgc cagggtggtg gaagctccag ctctaaggtc attgataacg aaattggccg
      481 gttttagctt ggtgggacca taaattttga ctccgtggaa atattccggt ttgtcacttt
      541 cgggattaaa ctggtagtaa gtttcaggat tatcaacctt ggggttgaaa aaattggctt
      601 tgacacccat ttttttgaga ttttcgatgt gttgaaaacg tgaggggtaa acccgttcaa
      661 taatggtgct acagccgaca gtttgagtga gaaggatgga aaatatggcc tgccaatcgg
      721 tcataaactc tggttctggt ccggtttcaa tatcaatatt ttttagaggt ttaacccagg
      781 aaacagtgat ttcgtctcga ccggggataa tttttgcccc catagtttca atggtattta
      841 agaaagtttg gacaatttcc gggttgctgc gcaaaatgtt aatagaacct ttagtggcaa
      901 gagcggcaca ggcaaaagta acaatttcat ttctgtcggc gatgacttgg tgattggtgc
      961 ctttaagttg gcggacgcca ttgataataa ttctttttgg gtctttgagg tcacggtgaa
     1021 tattggctcc catatcgtta agcatcgata tgagatcatc aatttcgggt tcctgagcag
     1081 agttgtcgag aatggtttcg ccgtgggcaa aggcggctgc cataataact gtttcagtga
     1141 cggtgtggct aggtttattg aaagtatagt gaaccgcctt gatcttgtcg gtattaaagg
     1201 tcatgttggt gttggtaact tcagttttta tacccatttt gtcaaaacag tcaaaaagac
     1261 gatcaagcgg tctggcgccg agtttgtcac cgccgggggt gggaacgacc gcttttccgc
     1321 ttctgactaa caaaggggca gaaaacataa aagaagttct agatttttca ccagtgccgg
     1381 caggaacagt agaattggta atttttgggg tagtaatctc aagacaatgt tcaccgcaat
     1441 agttgatagt cgagccgatg cttttggcaa tactttcagt tatttttatg tcagaaattt
     1501 ttgggatatt agttagctga gtggtttgag ggctgagaat ggaggcgatt atttgtttat
     1561 agctggcatt tttggctcca cgaatagaaa cttcaccatt taattctttg ccgccagtga
     1621 cgatatatct ggacatattt atttgaataa attaataact gatttaaaaa ttcgattgcc
     1681 ttcgagtcca ccagaactca tgtagattaa aatatcattt gatgtcagac tatgtttata
     1741 atactgcata atttgggttt tcagagggag atagattaat tttgacccaa gacagtcacg
     1801 aaaatctttg gcagtgtttc tgtttttttt gttgatatta tggttgaatt tgagttgagt
     1861 cagaacgatt tcatcggcaa gattaaaggc gttttttagt tcatctagac tacttttgta
     1921 ttgactaaaa gaagcatggg cttggtagag gacttttatt tttttttgag ggtaatgaga
     1981 atttagggca gtcaaagttg atttgatgcg gttgccggat tgggcaaaat catcaaaaaa
     2041 taaaatattt ttgggtttag caactaattc caggcggcgt ctgatgccgc gataggaaaa
     2101 aatagacttg gcaattattt tgggactgat ggcaagatag tcactgagag ctacggcggc
     2161 aagaatattt tcaaagttaa attggccaat aagagtagtg ttagttttga taatgcctga
     2221 aggggtgcgg ataatgaggg tggtaaaatt tttttgaggg attgtttttt ctaaaaaata
     2281 atcggattcg ggagtattgt agctgataac ttttgccggt gaatattggg ctaaactcaa
     2341 ggcagacggg ctttgttggt tgacaagaaa gacaccgtct gacggcagtt tttgaacgag
     2401 ttttttgaaa gtttttagat ttgaggcggc gtcgggataa gagtctttgt gttcccaatc
     2461 ggcgctggtg ataataagat atttggcggg ataataaagg aatttagcag ttttgtccaa
     2521 accatgaata gattcgtcac cctcgacgat agaccaatta gagtcggtta ttttgacggg
     2581 actgaattta tttttggagc tgccgccaaa catataactg ggcttaagtt tggtgtggtg
     2641 aaaaatccaa gccaaaagac tggtaatggt ggttttgcca aaactgccgg caacaataat
     2701 tgagttgggt ttgataatgt tttgagcgat atattttgtg gctgacaggt aaggaatttt
     2761 ttgttgtttg atttgtttga attcttcttt ggtattttca aatagattat atgaagaacc
     2821 gataatggct tgattaatat catgatcgat gtcagtgatg ttaattttta tatcggcttt
     2881 tttgagagta gtgctgatgg gagggaatat tttaggttgg tcggaaccgg tgactcgggc
     2941 accagcttgt ttgagagcca cggctaaact ggcggtcata gtgccggcaa tgcctaaaat
     3001 atgaatgtga gagttagata gagatggatt tgtcatactt tgtccactgg tgatggtgga
     3061 aatattcaaa gccaggcata tttcttttgt aataaccatt ttgtggagaa aatcggccca
     3121 aataacaata gtctaatttc agatcgtaac tatcaataat ggtttgcaag actaatctga
     3181 tgggtagatt accatgactg taatccgggt ggtaaaaaac ataggcaata tgactgatat
     3241 ccttggaaaa ataacaaatt gagtaagcaa taacattatt ttgttcatct ttccatatat
     3301 atagataatt aaaaatatgt ttattgaata cagttttgac agagcttgtg ggaaaatccc
     3361 agcctagttg tttaatccaa gagaaaattt gtttggtaat atccgaagtt agtttaaaat
     3421 ttttaagagg tattttttga taactgaagc cggcagtttt gttgagaatg cgacggtttt
     3481 cggaactgag cttaaattgt tttagatttg atcggcagct ggtgtcggaa taccaaaagt
     3541 tggtttgatt tctttggggc agcagccctt gggaataatt gatttcagga gactgattat
     3601 tattaagatt atattgaggc atcggcttgg cgtaatttaa aagcagttct aaaggcttcg
     3661 gcttcggacc agggatattc agtgttgccg tcaagaatgg tggtttcatg gcctttaccc
     3721 atggcaatga cagtatcttt ggcttgagcc agactgatag caagattaaa agcgtcctga
     3781 cggttgggaa tgttgaaata aacaaaatta tctttattgt ttttaattat ttttttgatt
     3841 tcggataaag gttgtttgtc atcgattaaa actgaatttc gggaaatacc actaatgatt
     3901 tgttggttta ttttgtcaat atcttcgttt ctggtgtcat cggcagtaac aaaggcaata
     3961 tctgccagtt gagagacgac ggaacccata atgggacgtt tggtttgatc gcgaccgccg
     4021 gtagcgccaa aaatagttag aatccgacct tgtctaacct gatggagaga ggaaagagtg
     4081 tttttgatgg cgttgggggt gtgggcaaag tcgatcaggg caagtatgtc taattgattg
     4141 acgacttctt cgcggcgacc cttagtattg ggaaaatttt tgacaacttc cagagcatat
     4201 tcggggttga ttttaagctg gtcgataatg gcaaaagcag ctaatatatt gtagatttgg
     4261 taataataaa gactatcagt ttttatttta aggctattaa gattaaaggt gagtgaattt
     4321 ttgtctaagt tgattttttt agcctgataa tctgatttgt gttcaatgcc gtaggttaaa
     4381 tatggtttgt tgccaagatt gtgtttgatg atttgatatg aagcgtcgtc tcggttgaga
     4441 atagagattt ttgaatgaga aaaaataaga cttttggttt cgatgtagtg ttgcatatca
     4501 cggaaataat ccagatgttc gtgggaagtg ttggtgataa cagaaacttc aaaattgcag
     4561 ccgtaatagc ggaattgatc tagggcatgg gcggtaatct cgcaaactac atgggtaaca
     4621 ccagcatcaa ccattctttt gaatattttt tgaataactg aggggtcggg gctggtcata
     4681 tggaggccgg tatcaatttc ctcgttgcca tatttggcgc cgatggtggt aataacgccg
     4741 gcgtttatgc ctgatttttt gagaatttca tacaacagga ttgaagtggt ggttttgccg
     4801 tcagtgccag tgacaccaac gagaaccaat tttttggaag gttgatggta gtagtgacgg
     4861 tagaaaatac ttttggggag atgaaagagg cggtttttga ttttttgcca cattttgaat
     4921 gatttaatta taacaaacca ttgatttttg aggccatttt gaaccaaata ggggcggctg
     4981 tatttgaccc ccaggatgat gatttagggt tgttgatggt gacgatcatg gtgaactttg
     5041 gttgataaca gggccaaaat ccgatatatg agccgatggt ggcagagtca gtatagccgc
     5101 ccttgacggc aacttgggca gtgccgcttt tggcgcaaac ttcaagatct ttgggtttaa
     5161 agacagaaac ggctccattt tcgacagcgt attttaggat tgatttaatt tccaaaacgg
     5221 aatcatgact aaagacgcga gtttcttttt tggatttgat gggataaact ttacctattt
     5281 ggtcggaaag ggatgtggca actttgggat taaccaagac gccgtcgttg gcaatagtat
     5341 taaatgcctg aagcatatta atttccgtaa ccacaatccc ctgtccgaat gaagctgtgg
     5401 ctaggtcaat ttcgggccaa ttatttttta tggtgggctt aacttctccc tgaagatcga
     5461 tgccggtttt ttggtttaag cctaaacttt ggtagtattt tagaaaatta tttaaaccta
     5521 attgtcggat aatattactc atcccgatgt tgtcggaatt ttttatgatg tctttgagag
     5581 ttgattcggg gtgggtggat ttgtcccaat tattgatagt gtattgaccg atggtaagcg
     5641 gttgggaaca attgtggcag atataatcgg gctgaattga tttttggtcg agagccatag
     5701 taacaactat gggcttaaaa atagatccag gttcaaatag atctgagatg gctggatttc
     5761 ttgaagcact gggggtggcc acagttgaag cggtttgact ggtcatagca actatttggc
     5821 cagttttggt ctccataacg ataatcgaac cggaatcggc ctgatattgt tctattcctt
     5881 gagataattc tatttcggcc tgatgttgaa aattacggtt aatggaagtg aggatagttt
     5941 gaccatcaat tgagggagaa ctccagcttt ttttggtaag gatggttttg ccggtggcgt
     6001 cttgaggcga ccaagtaaaa ccggttcgtc cgctaagctg tttgttgtaa taagcttcaa
     6061 gtccgccata tccggtttga taaccatagt tgtcattggc aacaaatccc aacatatttg
     6121 aagctaaact gttttcgggg taataacgag aactatattt tttgaaaata agaccattga
     6181 tttcttgaag ctggtttttt tgaggttcgg taaaattagt ggaaaggtca acccattttt
     6241 ggttggaatt tgatttgaaa ttattaagat tgggattttg tttggtaaat tcgggataaa
     6301 caagatcgat tttatttaaa atatcatcca agttttcggt catatttggt ttgtagagag
     6361 aaagacgata attggtttgg tttagggcca agggaaaatt atctgaagaa agtatttggc
     6421 cgcgggcagg gtatttggtt tcaagtttgt aggtctgact gagagccttt tctttgagtt
     6481 ccgggctttt gaccacctgc caataaaaaa gtttgccagt aacagccaaa aaacaaagaa
     6541 taaatgaaaa taataaaata cgatagcgtt gagtcattaa aaatttatgt tgatagactt
     6601 aataatgggt ttgaggctgg cagaaggagt gctttggaca agatgggtaa tagaggtaag
     6661 gtcggcgact tgtttccaag ctgattcaac ttctagctga agctggtcgg cttctttttg
     6721 gtataaatca agctggttgt tttgagtgac aatactactg gaatagaaaa aagaaaaaac
     6781 tatttgaact atgagactga gaatgattat gccggaaaat attttagaca ttaaactatt
     6841 ttatacgatc taaggatact tgagcgggat agagggttgg attttatctc cgatagagac
     6901 ggtttgataa atttttgttt ttgaaccaag tgtttgtttc ggttgataaa ttgtttaact
     6961 aatctatctt cgccggaatg aaaagtaata ataactacgg tacctttggg cttaacaact
     7021 tcaaggcttt gggataataa ttgggttaaa tttttatctt cctgattaac ggcaatcctg
     7081 agactgagaa aaattttggt ggcgggatca attttggttt taatgtggcg gtcacggtaa
     7141 taatccctga tgatatcggc taatctaaga ccgtctttga tggcagattt ttgcctttgt
     7201 ttaataattt ttagggcaat gggtttgcta tataattcct gaccaagttt ggaaaagatt
     7261 tgaaacagtt ggtcaaatga gtaggtgttg atgatttctt cggcggtgag agtttgagtg
     7321 tcggggttaa gacgcatatc taaagattgg gaatcgttga atgaaaagcc tcgattgatg
     7381 ccggtctgct gattttgact taaacccaaa tcagcaatta gaccgtcaac cttggatttg
     7441 attttttgct taacgacggt gtttaactga ttaaagtttt ggttgattgg aaaaaaattt
     7501 ggggataaat tattgtcttt tattcggtca gcggcaattt ttagattgtt ggggtcaaga
     7561 tcaaaaccgt aaacaatacc gccccgcttg agaatttcta gggtgtgtcc gccgtgaccg
     7621 agggtggcgt cgacataaat atggtgtggt ttgacacaaa gagtatccac ggcttctttg
     7681 agtaaaaccg gggtatgaaa attcattagt tgatcaaata atcccctacc agagtgttgg
     7741 tattttcttt ggtgacggtt tcgggtgata ttttagttat tttagctgtg ccgctgccgc
     7801 tggaaaagac cgagtaataa ggccgatagt ttttgatgag aatgaaaaaa tcatttttgg
     7861 tggtggtagt ggcggtgata gataaatttt ctagactgag agataatccg ctattttttt
     7921 tgatatcagc tgaagtggtc aaagtgaatg gagtctcaaa ggaggcagtt tgattgtcgt
     7981 tggtgtagaa agtcagagaa agaactccgc caacaacgtc ttcatcaaat ttgtatttgc
     8041 aaccattagt gcagctggca gttcccaaaa gtaggtcttt ttcgaaatta ttggaagtta
     8101 gtttgacggt atcaccgaca cctttttcca tttctaaacc gttgtctttg gcagaataaa
     8161 tgagttcata ttcgacttgg ctgatgctgg caggaatttt gtcaactttt agtttgagct
     8221 cgtgaccatc agcacgaggg ataagagaaa ttataggttt gtcgctgtca gtaagagtca
     8281 gttctttggg agcagagttt ttggtttcgg cgggagtttt gggagctttt ttgccgcagc
     8341 cggaaagaag aagagaagaa agaaaaatta ttaaggtaaa ggaaatggtt ttgaacttat
     8401 tcatattagt tatttaacct tttttcaagc caggtgtcaa tttcatcgga tttgagacgg
     8461 atttgctcca tagtatcgcg gtcccgaata gagacagtgt tatcttctag ggtttgataa
     8521 tcaactacca gacaccaggg ggtgccgatt tcgtcttggc ggcggtattt tttgccaata
     8581 ctgctactgt catcccagtc gacagcatat ttttggttta actggttaaa aatagtttcg
     8641 gctttggtaa ttagttccgg tttgttggcg gctaggggga gaatcgccat tttgtaagcg
     8701 gcaattttgg ggttaatttt aagataggtt cggatgtctc cggttgaagt ggtttcttct
     8761 tggtaggcat caagcaggag aaaaaagaag ctgcggtcaa ctccgccgga agtttcagtt
     8821 atccagggga tatatttttc ttgagtttga gggtcggtat aggaaaggtc ttgtccgcta
     8881 aatttggcat gttgtccaag atcgtagtca gatcgccagt gaattccttc catttctgcc
     8941 caaccccaag gagcttggta ttcaatgtca aaggcttttt tggcatagaa agccaattcg
     9001 tctttttgat gttggcggaa gcggaggttt ttggaattat taaaatatgt ttgataccaa
     9061 gccatgcgag tttctttcca gtattcatac catttatcca tttctttggg atggacaaaa
     9121 tattgaatgt cccattgttc aaattcgcgg gtgcgataaa gaaaattttt aggagtaatt
     9181 tcgtttctaa aagctttgcc aatttgggca ataccaaagg gaatttttat gcgggtggag
     9241 tcaacgatat ttttgaaatc caaataaatg gtctggcagg cttcaccccg gagataagca
     9301 tgttgttttt cgccctcgat aacaccaatt tcggtttcaa acaaaatatt atattttcga
     9361 ccttgagtaa attgaccacc acaatcgggg catttgctgg ggtcgtcaag aagatcgggg
     9421 cgaaaacgtt tgtggcattt tttgcattca accatagtgt cggcaaaatt tttgacatga
     9481 cctgaggctt gccagacggt ggggtgagtg atgatagaac catcaatgcc gacaatatcg
     9541 cggcggcgga caacattttc ctgccaccat aaattcttcc agttaagttt catttgggaa
     9601 ccgatagggc cgtagtcata aaaaccttgg acacctccgt aaatttctga gttttgaaag
     9661 attattcccc gacgtttgca gagactgaca attttgctaa caagatcgct ttgattagtc
     9721 accatatatc cttaaaaatt aatattaatt tttagggacg aaaatttctt tccgcggttc
     9781 caccctattt gacagattat ttttgaacat ccgtccactt tataaatttt caactatcga
     9841 agtgccttct tcgttcgtag ttgataataa taggtatatt ttaccatcaa agaacagtga
     9901 taggattatt caaatagata tattttctga ttttggttgt cgataaaaga tatttctgct
     9961 ttttgaggat agtgaaaaaa ttccaatcct ggaatttttc tggtctcatc aaagtttttg
    10021 atatttacat tccctaccca gcgataatat ttgtctggga cactaaaccg tagcctatca
    10081 ttaatcgttt gttggggaat tttagtaata tcttgaattt gagtgagatt tagtccccag
    10141 tcagacattg atctgggggc cataaatgct tcaatagcgg ctatttggtt taatttaggg
    10201 acaatattaa tttgatattc tggtttgtat ctattaaata gataattcca atcccaaatt
    10261 aatttccact ggttattgat tttttggaag attatgttgc ttttattttc gagaggtcca
    10321 tatcgggttt ggtaggtggt attgagcact cccgtaacaa tatcttgatc gaatttgata
    10381 gaagttatat ttgattcgat acttctggtg ccaattttat taagaaaatt ttcataagaa
    10441 tttataaagt agtcttcgtt cattaagttt cgttggtgta gagatataga cctataaagc
    10501 tctcgataat ttctggtatt aataaatcta gtagtgctct ctatggttcc ttgatatggt
    10561 tgtttaataa cagaagtgta atagaaagga atgtttaaaa taattaaaag ataaattaaa
    10621 aaaggttttt gctgcaaaca aatagtaata ataagacaaa aagtaggaat tattggcatt
    10681 aaatatcttg gaaagaatgg tataaagcaa ttaattaata taaatgatag tgaaaataga
    10741 cacagataaa gagttaattt atttttgttt ttaatagaag taattagtag gacaatcagg
    10801 agtagtagtc cagccgttag cattggggac cagtcaccaa gcgtgccagg ttgagcccca
    10861 gcccaaaatc cttgataacg atttattaag atacccttga gttgattaaa ataatctata
    10921 tgactattac cttgtccaat ataaaatctg ataggctttt catgaagtct taaccatgga
    10981 attggatttg ggtggtagat aaagtaggat ataaaagaaa aaccgtagcc tcctaaagtg
    11041 ctaaaagcat aaagtagtat ttttttaatt tttttagttt tggtggttaa gataaaaatt
    11101 agaccaatta aaaatatcaa gggagaataa acgcctatct tgcatccggt ggcaatacct
    11161 aaaaatattc cggcaaatat tagtttttga agattatcta attgaagata tttaagaaaa
    11221 aataaaagat gaaccagaaa aaagaacatt aaaggcaggt caagcatggt ttctctgact
    11281 tgggtagaaa taaaaggaga tgtagccatg agaagtagtg taaggagaat tgtgtgggtg
    11341 tttttttgga aaaatatttt agaaattaaa tgtaaaataa taaaagatga aagataaaaa
    11401 ataatgctta ttatgtaagg attgctaaaa aaccgttcag ctaatccata aagcaatttg
    11461 gcaaagggtg gaacttcgta attgatatta aatggatttt caccgaaggc tagcctgtaa
    11521 ccaacaaatt tatataaatc tccatcacct ataccgcgaa cactgttggg aatattccat
    11581 tgggagtggt aataccagtt ttcgtagtat tcaggctcat atttgtaaaa aaatatctgt
    11641 cgggaataat aaaaagttaa agaaataaaa atcagaatta gtgtttttat aatgttgttt
    11701 ttcattcctt attgtattta atcatttttt agattttttt ttgggtttta tctaaacaat
    11761 ttgttgcttt ggagaggttt ttcgataatt gattcaaaaa atcttttgat tagtttttgg
    11821 ctggtcttgt aatctttttg actaaaatat gtttttatgt ccaaagggac cccaaaaccc
    11881 agagtttcca aaaagtagat ttcgttttgg atatactggc ggattaaatt tttgttgata
    11941 gcagcaataa tattttggga aatttcaaag attttgtcga tatgctgatt ttcggcgaca
    12001 aataaattga ctatttccaa aaagtaaaaa agaaggttaa gttgggccaa atttttgtct
    12061 gtttggagaa aaggagaaat attttgtgat tctgaaatcc aggtaaaatc tcctttttgg
    12121 tatagttgag ctttgatggt gttgcctagt tggagattgc caagacgact gctttttatg
    12181 tttttagcgc ctttggcaat agctacaatc ttgcccattt tgggcgttaa aagggtaata
    12241 agcaagtcag actctttggt agtttttttg ttgatgacaa tccctaaggt tgatttgtag
    12301 tggtccattt agagggtaaa tctaatggtt ttgtcggcaa cccaggagaa gttttgcttg
    12361 gagccgttaa ttatcagctc ataatcaacc ggtttggtgc cgggttgttt ttggagcata
    12421 agggtgtaaa cggaaccaat ttgagcgccg gaagtatatt cgattgaggt tttgaggttt
    12481 gattgggggt taagcccaaa cttgttgcca taaaaacctt caaagacctg tttatttaac
    12541 tcttcatact ggacagtttc aacttcagaa ccggtcattt tggtgagttt tgaacctttg
    12601 ggggtataga aacggaagat atctcggtag gtagaagcgt taagacacag accgcctttt
    12661 tctagattgc aattggaacc aggggcggaa ttggtataag taatatttat tttgtgttga
    12721 gcttgaccgt ttttgatagt gatgtcgtgt ttgatttttt gagtaatgaa aatattgctt
    12781 ttggcgctgg caaaattggt gtcattaagg tggagatagt caaccgagga ttcggtgtcg
    12841 tatatttttc cggtaatgtt ggccaggtca cccatttttt gattgtcttc gttgggaaag
    12901 taaaaaagga cgtgtttttc gttgatactt ttgaagccgg cttgaatcaa ctgacccatt
    12961 ttgttttttt ccgaacccat aacgttggca aggagggcgg acataagcgg gttcataaaa
    13021 tcttttcgat ttttttcgat ataatcccga gggcgaccgg acatccattg catttcgtaa
    13081 ataatttgag gacagccata acaccttttg tcaggttcgg tagtaaaagt gccgacccgg
    13141 gtgtctagtt tacccaaaac agcaaccata tcaaccaaaa gttgggtgtc gatggcgata
    13201 atggcatcaa atttttcctt actgccggta gctttgagat attggtccaa aaatattttg
    13261 gcatcagtgg gaaaatcggg agaaagattg ctgtcgcgga gattaagata aggaacattg
    13321 atatggtaac ttttgataag cctgggagcg ggagtgcggc tgttgatgcg gtcgtcaaga
    13381 tcataaatat tgctggcagt gccgggctta actttgccat tatcgaccgt catggtgctg
    13441 taagcagtcc aaaaaccacc actaggtcgg agctcaccat cattttgaaa gatcatgaga
    13501 tattttcgag gagaatcttt gcccaagagc caagaaactt tggacaaaag cggcttgccg
    13561 tcttttataa gtttatggga ttgagatagc agctcctgag ctttggtaat attagatttg
    13621 atttggtagc cgcggaattc ttcgggatat ctggagggat ttatttctcc ggccgatttg
    13681 tcaatttcgg caattttggc ttcgatggtg tctaaatacg gaatcaaacc ttcaacagat
    13741 tgggtcaaaa aggcgattct gtcttgagtg gtttcttcgc tggaagcggc agagccggtc
    13801 atacccaaaa aatctttgta gggttcgatg gcttttatga taatatcgct ggtatcaagg
    13861 ctgtttttgg caatactgat ggttcggaga ccatcttggt agtaattatt gattatagga
    13921 aggaagccga ttggttttag tttaaaatag gtgtcttcga tagatttagt gtgatctttg
    13981 atgacactga gattaatttt gattttttcc agatcttttt cgttgatgga ttgtttgaga
    14041 tgatcgactt cggttttggc agcttcgatt ttggtctgga gttccttggc ggggagatag
    14101 actacaaaaa atagtccgag agcaaggata aaaataaaag caaaaatgat agcaataatt
    14161 ttgggccatc gccgattacc cagctttact ttcttacccg gtttggtgtt ggcggttttg
    14221 gtgatcaaat ccatactctt agtttaacaa agatgaaaca atttaccaac gagagatcat
    14281 agacatggga gttttgagaa tgatgagaag ttcctgtcta atagattgat tgcggatgta
    14341 ttttgagtcg agtttggctc gttgttcaaa attaagttcg ttgcggccgg aagtttgcca
    14401 aagaccggta atgccaggtt tgacggatct aatatcgtca atatattttt tggtggtagg
    14461 atattttttt tcgtactctt gaagttctcc ttcacgatag gctctaggtc cgactaaact
    14521 catttctcct ttgagaacat tgaccaattg aggaaattca tcaactgatg aagctcggat
    14581 aattttgcct atgggtgtga ttcgagggtc ttcggacata ggtaatttcc agccgccgtc
    14641 tttgtatttt ttccataact caggataatt ttcgcggaga cgtttgtcat tgtcgccagt
    14701 atacatagag cggaatttat acatccaaaa ttctttattt cctttggtga cccgcttttg
    14761 tttaaaaaaa ataggaccag gtgaagtaat tttgacaaga atggaagtga gtaaaaatat
    14821 gggagaaaag ataatcataa gaaagacaga gctgataatg tcaagaattc ttttgaagac
    14881 gttgaagtag agtttgtctg tcatggtttt ttgagaaaaa atgatttata ttttttgttt
    14941 ttgaccattt taccgattag gtcgcgaaca tgataactgt cgtcaagatg gcagataaga
    15001 agaccatcat cagtgtcaat gacggctaaa ttactaacac cgaccaagcc aataagctta
    15061 tttttggatc cgtttattaa acaattagtt gagttgtggg ctagatagtt ggtttgggaa
    15121 ttgaggacgg cgttgttgtc ggagtctttg tctgatcgtt ggtgaatgga tttccattcg
    15181 ccaatatcgc tccaatcaaa actagcaggg ataacataga tttgatcgct tttttcggag
    15241 acggcccggt caatggctaa gtcgggtgat ttggcgtaaa ccttagcaat agtttttttg
    15301 tcaagttgag aattgactaa ttgttgataa agtcggaaat attctggttg gagtttttca
    15361 acttcattga ctatatcagt tagggtaaaa gtataaatgc cagagttcca gagagcgcct
    15421 tttttgatga gattttgggc ggtgtctttg tctggttttt cgacaaacaa actgacgttg
    15481 gacaatggtt gatttttttg gcggggaaga atgtagccgt aggaagtgtt ggcaaaagca
    15541 ggcttgatac caatagtgat aatccgatgg tgattttggg ctagtttttg ggaattggca
    15601 atatcttttc taaaactgat tatttttttg atgtagtgat cagcaggagt ggtggtaacc
    15661 acggcatcag gattaagttt ttggatgtgg gctaaggcca aaagaatggc catggtggta
    15721 ttctttttct ccggctcata aataaaattt tggggtggaa gtttcttgcc caagattttg
    15781 tcaattttgg cttggtattt ttggttggag acaataaaaa tgttttttcc gggacagaat
    15841 ttttggcaac gagaaaaagt ttgttctaaa agagtttttt tgcctaaaat tttgagaaat
    15901 tgtttaggat tgcccgcatg tgaaagcggc cataaacggg gaccactacc gccgcagaga
    15961 atgactacat actggtttga tttagccata gttggttgat tgtttgctta aagtttagca
    16021 taaatttttt gcgggaaaat ttggcggcat tttgaacaca gtcggcggga gaaaaatttt
    16081 tggaatcaaa tttggctagg gtttgggcca aagaatcagc tgtttgatga tcaaaaaata
    16141 caccggtttg gtgattgatg acggtttcgg ttagaccacc ttggttgaag gcaatgacgg
    16201 gtttgccaca ggcttgagct tcaagtgggg taagaccaaa gtcttcaatt tgggggcaga
    16261 tgagagcttg gcagttttga tatagagaaa gaagtttttt gggagaaact ttgccggcaa
    16321 aaataacttg agagttggaa ttttgggcgg ctaatttggt taatctataa taatcacggc
    16381 cctgaccgac aataatcaat ttttgattta gctggtggca ggcgcggata gcaaggtcaa
    16441 tttttttgtg ttcgactagc cgggaaacaa ctaaaaaata aggtgaaacg gtaggattgg
    16501 ttttggggat aaaaaatgaa gtgtcaactc cgggataaat gactttggcc ggtctgttgt
    16561 aggcggattt tatccggcgg cggacggttt gggaattgca cagacagtaa tccggtcgct
    16621 gcccgtaaat aaaatcggtt ttttgatagt gtttaagaat gggcttaaga aatttaaact
    16681 gttttggagt ttggtaaaga taacggttaa cattgtgaaa ataacagacg aacaaagttg
    16741 acggcggggt caaaagagag tgaccgacgg tagaagtggt ggaaataaca atatcaaatt
    16801 gagaaaaatc aaatttttct agggccaggg ggtaaagagg ggtgtagatg atggggtttt
    16861 ggtgacttag gggaagatgg ttaataaaag aagggattac tttgattgag gatggcagcc
    16921 aagaggtttt tttggggtga tagtttaggg taaagacggt tgcctgggga aaaatttgta
    16981 aaatatcaag aaggaccttt tcggcgccgc cccactggtt gagccagtcg taaaaaacag
    17041 ccaccctggg tttagcggtc atttagggtt ttttggtaaa cagataaggt ttttttggca
    17101 gtattgtccc aagagtattt gtttatctga agacaacctt tttggataag ctgccggcgc
    17161 aggttgggag ttttgagcaa ccgagttatt tgtttgacta attgactagg tttatagggg
    17221 tcaaaataaa gaactgagtc ttggtaaatt tcgggcagac agctgctgtt ggaggaaata
    17281 accgggcagc ccagggccat agcttcaaga ccggtgagac taaaaccttc cataaacgag
    17341 ggatggacta gagcaatagc ttgggagtaa attcgcttga actgactgtc aggaatataa
    17401 ccgagaaatt cgatttggtt gctaacttgg ttttgtttgg cttcggcttc gacttgatga
    17461 gtaaaaaaac ttcgggcgct gattattttt aattttagtt ttggaagtga tcttagggcc
    17521 tgaatgataa ctttgatatt tttgtggggg tagagattgc cggtatagag aagatagtcc
    17581 cctactggct tgcttttagg attaacttga gagagatttt ttttgaaatc aggatcgacg
    17641 gcttcatgag tggtggtgat tttggcggga gaaagatgaa aatgtttgat aatatactga
    17701 cgccaaaaat cactggggac gataatagta ttggttttga tggtgatgtg agtaatcaaa
    17761 atgtaaaaaa aatattttgg ccaataaaga agggaatttt tggtagtatt ggctttgcct
    17821 cggaaaaaat gtttaatgag gtcgtgaatg gtgacaaccg atcggccaaa ataaagcaaa
    17881 ggtttgttga aatgggtaaa gtggactaag tcgggtttga gtttggagat gacaaggggt
    17941 aaaagcaact gttctttgaa agaataatga cggatgttgg tggggatata attgaaacta
    18001 tttccaagat cggttttgat atcggaaaga agttcagggt aaacaaggag actaaagcgg
    18061 tagtgagaga aatcaggttg gtgagtaaga gacaataaaa catttttggt atagcgaccg
    18121 atgccagtat gtttggggcc gtaaagcctg gcgtcaataa caatatgctt tttggtcata
    18181 aatatttggc tttgaagagg cgtttgattt ctagaagttt aataccacca aggactagtt
    18241 gccgggtgaa aaaattatat tttgaaaaca gatttcgttg ataaaagatt ttcatagctt
    18301 cggtgctgga tttggcggcc aaaattttgg tgctgcggtt ggctgaagag agtttttgac
    18361 tttgtgattt tatgccggaa gaaatgccct gaaaatgggt aatctgacag tggggattga
    18421 aatacaattt atattttttt tggtaaagtt ggtaacaaaa atcaagatct tcgccataaa
    18481 agaaatatga ttcattccac cagtttatgt tttgaccgac agattttttg accatgagaa
    18541 aggcgccgac acaggcttcg atagggtgaa tggtggaagt gtctaggtga cccagaaaat
    18601 aaccgttaaa aaattttgac ttggggaaga gtttggaaat accgctaaaa taacaaaatg
    18661 aacgccaagg agtgggaaag ccgcgatggc attccggctg catttgaccg gttttggcaa
    18721 gtttaatata acaagtggcg gcggaggcgt ctggctggcg gttgaaaaag tcaatcatgg
    18781 tttttagagt cttgggttgg acaatggtgt cggggttgag aaaaagaaca tacttagtgt
    18841 tgggattgat aattttgacg ccttgattgt tgccagcagc aaaaccaaga ttttttttga
    18901 gaaccaaata tttaacccac ttatgtttgt ctttttgggc taatttaaga ctaaggtcgg
    18961 tggaggcatt atcgacaaca atggtttcaa tggcgtaatt gcccagattt gatcgggaaa
    19021 tgcttttgag acaacggcga agatagtcgg cagaattata gttaaggata ataattgaga
    19081 gatcaattct ttttgacatt ggtaatcttt tggtaaattt tgttcaggcc atcaacacta
    19141 ttttggtagg agtatttttt ggcgattaat ttgttggctg cctcccccat ggcctggcgc
    19201 tgatggtgat ttttgagtaa attgatagca tttttgggaa cttgatcata atcgcagaca
    19261 atggattctt tgaaattttc gattttgata ccacccatac cttcgggggt ggtaacgatg
    19321 ggaagacggc aggccatagc ttctaaaaac ttgtttcggg tgccgccgcc gctgcccatg
    19381 ggagccaaaa gtatccagca atattggtaa aaatacatag gatcttgtgg ctgaccgtca
    19441 aagtcagctt cctgaacaat aacgtcgggc gattggtagt gaccgtaata gtcgggagca
    19501 tgtcggccga tgatgtaaag tttacagtct ggaatgactt gtttgatagc cggccaggag
    19561 tctttgatga tcatttcgac tgattcccgg ttttgcatcc atttcatatt ggaaactcca
    19621 aacaagacgg agggaaattt gctccggggg gtcttgggtt tggattcaaa aaatttattg
    19681 tcaactccgt tttgaaataa ttcaatgtcg tttcgcccgg taacttggga aatcagctca
    19741 cggtcttctt gggcaaaagc gacaacggta tgtgtgtttt tccaataatg agtttcccaa
    19801 tatttgagtt tgagaacgtc aatccagaaa attggtttaa agattttttt ccagccggaa
    19861 agagtttcaa cgtagtgttg ataaaccgca tattcaacgg ttaaatcaac caaaacgatg
    19921 gggattttgg ttttgggtat attgggcatg ggatagaaac aatcgcagtg aatcaaatca
    19981 aatttgttat tatctagctc gtgtttgatg gcttgacgga agtttttgtt gatatgtttc
    20041 atgacgagga acggatattt gcttagaccg gttaaaagaa cttttttagg atcccaggtt
    20101 ttgccccgtt taacaacgat aaccttggaa caatattgtt taatgtcttc taaaccttct
    20161 tcatcggggc tgaggcaaat tagggtgata ttgttgtcgg gggagagata tttgatggaa
    20221 tagaatgagc tgttttgacc gccggattgg gacagtcggg ggatataggg agtaatcatt
    20281 aaaatgtttc gtttattttt ttgagtcatt ttttagattt aaagattgat aataacagcc
    20341 taacataatc caaaagaggt aagcggtttt tgaagcttca aaaacatcaa taaaaacggc
    20401 atttgccaaa gtggtaataa gaccaccaaa gcagattaag gaaatcatat cataggtatt
    20461 ttttgatttt ttgaaaataa gaggaatggt tttgataaaa aagtagaaaa tgagagcaga
    20521 aaaagtgata agtcctaata gtccgctttc acccaaacac ctgagataat cattgtcggt
    20581 agccagagtg attgacccta aaccgctgcc gacaagtgga ttgcggcgcc aagcagtgat
    20641 ggctcggggc cattcggcat tgaagcgaat ttcgccggat ctggcaacgc caacgtcggc
    20701 atcaacagga ggataggttt ccggttgatg tctgacaaca gtgggagtgg gtttgataat
    20761 ctgaccggga atgggggtta actttggggt ggatatggtg ggagtaggaa taatagtggg
    20821 ggcgggagcg aaattatttg aggatttaaa ctggtttttt agggcgggaa tggtggcaat
    20881 gaggcgttga ttaagatcac cggaattaaa aatactgaaa ataactaaac cggtgacggg
    20941 aataatccaa aaatattttt tgataaaaat aagagtaaga caaactccac ccaaaaaggc
    21001 aaaaattgaa acccgagaag cggttagagt aagaatatag tatgacggta accagactaa
    21061 taagctgacg attttggtaa atttattttg gcttaagaga aaaattgcca gaataataat
    21121 aagagcgacg ctgagaaaag cagccaaatc ataatgaccg gcaaaagtag aactgattcg
    21181 ggtccaaata ttcatctgca gcagttggcc tttggaaaat tctgaattca tagtggaaac
    21241 cacaggaaaa tgaagatatt tttggccata accataaaaa gtgactaatc cggtggcaat
    21301 aaggaaaaaa tagtaaataa acttaaaatc acttatggat ttgacggcat caagggtaat
    21361 aaaaaataaa gacatatatt caaaacggcg gaaaaggtgg agcaaaagaa tattggtagg
    21421 ttcggtctgg tagataagat aggcgtgaac aaagctaaca aaaatagcta aaaaatagag
    21481 aaaaaatagt ttggttattt ttagcttgag aaaggaaaaa ccattggtta cctgataaat
    21541 aaaccaggtt aagacggcca aggcaacgat gatatcatcc agacggacag caacataggt
    21601 attggggaca ttaaacaagg gaaatttagg ataaagagga ataaaaacga tgagaatggc
    21661 aataattgat ttgatggggt ttatattttt gaacatgctt gaaggtaaac gggagaaaaa
    21721 tgaataacgg atctgatgag ggcgttgatt ttgagtaaaa atttaaccag aggatactgc
    21781 catttgggtt tgtgcttttg gaaaaaggag agaaaacctt tgtattcgtt taagattggg
    21841 tcaagttttt tggcggctga agccctgccc aaatgaatta tctgcggacc aataagatac
    21901 cagacgtgat tgttgggagt agtttggctg attctgtagg aaagttcaag ttcttcgcca
    21961 tacataaaat aattttcatc aaaaccacca actttatcta agagtggttt tcgggtcagg
    22021 aaaaaggcac cggtaaccca gtcctggcgg tgatctgaaa gataaaacct gtctttggta
    22081 taaaattcgg gggtgtgagg gtgaataggt ctgataattt tgttgaccaa agggaggtca
    22141 tcaagattta gacaccaggt aaaaacattg ccaaggtttg ggaaaaaacc tccggaggct
    22201 tgaatagttt tgtcttgatt taaaagctgg gcggtgcagg tggaactttc cggatgggaa
    22261 cagagccaat cgagtgattg actgatggcg gaatgaagaa ttagggtgtc ggagttgaga
    22321 aataaaatat agttgccgga agcaattttt aagccttgat tgttggcttt gccaaaacca
    22381 tgattggttt tgttggcaat taattttagg gaaatgtttt tggatttgag attgattttg
    22441 aagcgattaa ttttagtaag gctgtcgtct tgggaattgt tgtcgatgat aataatttcg
    22501 tagtcaattt ggttaagacc tttatcctgg ataattgatt tgagacaatt tagggtaata
    22561 tcggcggtgt tgtaggaaac tataatgata gaaagaatgg gattaatcat gtttgataga
    22621 gaatttaagc accaaaacgg tagtaattag agcaatttcg gtgataaaaa aggtaataat
    22681 ccgaccggga atgtcggtag gaaaacgacg gagagtgatt ttggcgtgat attctttgcc
    22741 ttcgtagatc aatttgtttt tgatgcggtt ttcg
//
LOCUS       DAOSHV010000003        18030 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_2526_length_18030_cov_5.571961, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000003 DAOSHV010000000
VERSION     DAOSHV010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 18030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18030
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2526_length_18030_cov_5.571961"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1802
                     /locus_tag="PKZ29_00345"
     CDS             <1..1802
                     /locus_tag="PKZ29_00345"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOG37352.1"
                     /translation="PIPTSYPTTIPTDTPIPTIIPTSTPIPTNTPAPTNPPQPVIRST
                     STPIPTIYPEVLHLSAYRVELYPGEQQEIKATIIPSGVFNNTVTWTVENSHVARIESA
                     KNRLIITAATPGTTKIIAKTINDKTAEILITIKDTRTQPTPSVSNVTLTSPTAAASTP
                     IPTRAIIEPSSISINQPQLSLETNRTIQLTVTYTPDTTNRRHLNWSSSNISVATVDVA
                     GRVTAVNPGTATITASSVNGQTSRSTITVPGTNLPLPTSTKTPTSVPTRTPTNIPTQI
                     PTRTPTRIPTPIPTTAIINPTGINLSATSVKLYSGEIQKISASVVPANAANKSLQWSS
                     NNPTIATVNSLGYITAKTPGTTTVVVKTSNNITKSISVEVNRIPVPTIIPTLPIINDK
                     QGTITHESYGGMQYILYLPYGFTKNRDWPLLVYLHGWEREPNINNMYKQGHIPRLLRD
                     GNNYNAVVVAPFSTRSWGYSSNQQQAKAIIDHVVSQYNINKSKISLTCHSDGCWGGTA
                     LVRNNSTFFSSLVAVASSAPSSNSASGFTSTKIYYWYGTSDADSTNRSAFVNSINQAG
                     GQAYIKSIAGGHNIMKQVYIDNKMIEWMLSQSR"
     gene            1855..3849
                     /gene="uvrB"
                     /locus_tag="PKZ29_00350"
     CDS             1855..3849
                     /gene="uvrB"
                     /locus_tag="PKZ29_00350"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOG37353.1"
                     /translation="MSFNLQSDYQPSGDQPRAIAKLSHGINSDIAHQTLLGVTGSGKT
                     FTIANVIQNTQLPTLVISHNKTLAGQLYQEFKELFPANKVSYFVSYYDYYQPEAYLPA
                     SDTYIAKEVDINDKIDRLRLEATSNLLSGSDNIVVASVSCIYNIGDPNEFEALTLLLQ
                     VGQNYPRRQLMENFISLFYTRSELEFKRSTFRVRGDLIELWPSYTDSFLVLEFNGNYL
                     AAITQRHPFTATEKKFDQFKLFPAKQYVGGYQNLDNIFAQIRHDRDQQVTMFKSQNKI
                     LEAQRINQRVEYDLEMLQETGYINGIENYSIYFDKNRQHGDPPYTLVDYFHRLWGKNF
                     LTVIDESHVTVPQIGGMYAGDLARKQNLINFGFRLPSALDNRPLKFDEFADRISKVIY
                     VSATPAVYELEKSKHHIVEQIIRPTGLVDPEISVRSTNNQIPNLVEEIQRRVDKKERT
                     LVITLTKRMAEDLSSYLSDSAKTGHNFKVAYLHSDIVTLERSSILDNLRSGDYDVLVG
                     INLLREGLDLPEVSLVAILDADQQGFLRSQSSLIQIMGRASRHISGHVILYADTMSPA
                     MTGAIKEVNRRRQIQLKYNQDHHITPKSVVKSIRPQIIEAVVPEEKPEWFKIDVSSLT
                     PNQRQKQIRELRKQMRNLAFNLDFEEAIKIRDKIREIETF"
     gene            3865..6534
                     /gene="uvrA"
                     /locus_tag="PKZ29_00355"
     CDS             3865..6534
                     /gene="uvrA"
                     /locus_tag="PKZ29_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008287923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOG37354.1"
                     /translation="MIDKIIVKGARENNLKDVSFEFPKNKLVVFTGVSGSGKSSMAFD
                     TLFAEGQRRYVESLSAYARQFLGNMKRPEVDLIDGLSPSIAINQKAISHNPRSTVGTI
                     TEIYDYLRLLFARIGHPHCPICDQEVAPQTSRQIVSQIITIAQHQVQGLNYYRFLILS
                     PVVRDKKGDFKGLTDNLKKQGYKWLRVDNQIIDLYSDFGIAKTNKHNIDVVIDRISIN
                     KDILKDQKPLTDRLIDSVEQGMKLANGLVIISEVLSSSFDFPDFPKDFHDTLYSENFA
                     CPNCNISLPEIEPRLFSFNSPQGACPECKGLGSKYQIDRSKFPDWRARMMESRYVNSG
                     SDAIRDELEKFMIKTECQLCHGSRLNQDALSIHILSKNIYQITQYPIEKLSLWINTLN
                     SKLTSDQEKEILIPIKQELTARIEFLLAVGLDYLSLEREAGTLSSGESQRIRLASQIG
                     TGLTGVLYILDEPTIGLHSRDNNRLIETLKKLRDTGNTLVVVEHDEDVIKSADHIVDF
                     GLYAGKKGGQIVAQGSIDDIKKSPQSLTGKYLSGKLKIKSSQNPVSQQLASNRISITN
                     ARQWNLKNINVDFPLNKLVCVTGVSGSGKSTLVHDTLYGGVRKAILGSYYGTIGKFDQ
                     ITGAESVSDVLLVDQSPIGRTPRSNPATYTKIFDDIRQLMSQTIEAQLRGYDASRFSF
                     NVKNGRCPVCEGQGQIKIEMQFLPDIYVTCDECHGSRFKAETLEVEYKGKNISQILNL
                     TIDEAADFFKNIPKLKRNLETIQSVGLGYLELGQSSNTLSGGESQRLKISRELVKKEN
                     GRMLYILDEPTTGLHFYDVDKLITVLRRLVDKGNTVVVIEHNLDMIKNSDWIIDMGPE
                     GGDKGGQVVAVGTVEDIVNCTKSYTGQYLKTHL"
     gene            complement(6537..8054)
                     /locus_tag="PKZ29_00360"
     CDS             complement(6537..8054)
                     /locus_tag="PKZ29_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37355.1"
                     /translation="MTAKEKWLSLVFGIIWVISAVIRFGQVWNYQTAFTYDQSRDLLD
                     IRALGEFRDLAVLGPTTSINGLRLGPFYYYINLPAYWAGGGNPQTLVNWNIILFLATA
                     GFIFYFFRKKNIVLGFIVGSLFLMAPQMFNVTRYFWNANMAIYLSVYFYLAVWNFLEK
                     KDKKSIFWLGITSGLLTQFEAAFGVVCILFAVVIIVINKKLGWWKNFLIGLIPWFLPQ
                     IALEIKNRFQMTKLLLGMISGQNQVLGEKLLLTETVSSHLKSMIYFFEGQLILPYGWG
                     IIVFLVAVGLILANKKYRRYGLYFLVFWLLALGFYIAVYHHPLKMWYLESLRVWYVLV
                     VGIGIVNISKYQKIIGIILGVFLIRNFWLTVVDQSKFMNKTGSDDPKNLANLIRNIDW
                     VYEKINGDGFEAYTYVPEIYDYPNQYLYWWYGVKKYGYMPQKVSYSLAQVPEYVRSQD
                     EFYRMVKKNDKSKIALVYEVKNDYVGWLDQFKDYCVTDKLETNWKTMVEIREKCK"
     gene            8077..9348
                     /locus_tag="PKZ29_00365"
     CDS             8077..9348
                     /locus_tag="PKZ29_00365"
                     /inference="COORDINATES: protein motif:HMM:NF020048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HOG37356.1"
                     /translation="MIDLTTIPSSPGVYIYQNQQKEIIYVGKAVNLKKRVLQYFQNRD
                     ALGYKTRQLVSQIASAKFITVDSEIEALVLESGLIKQYRPKYNSQLKDDKSYIYITIS
                     RDKFPLVRPAFKSSLNDNDIIYGPFPDSRSVKSLLKTIRQIFPYYSAKHGLKSCLFCH
                     LGLCPGPAPDPKEYKANISKIKKILTGHFHKLINQLKTEMKLFSQAENFEMAKVRRDQ
                     INSLEYIISGWSRLTHLYQQVDLPEDKASKAIAELILVLKDYFPQLTSLNRIEAYDIS
                     NLGSKYFVGSMVVWQQGRIDHDQFRQFKIYSKVTPDDQFMIKEVLYRRFKHPEWQFPG
                     LILVDGGKPQVSAATKLIDELEKLYNFKLSIIGLAKKQETIVIKTNIDWVEINLPKNS
                     PALQLLQQLRDEAHRFANHYRRQLIRRSTLS"
     gene            complement(9334..9942)
                     /locus_tag="PKZ29_00370"
     CDS             complement(9334..9942)
                     /locus_tag="PKZ29_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37357.1"
                     /translation="MSKTAEGLNPTAYGLPANELPHRSDLTAMPATDTGARQIGLVFE
                     NGQPTTEMRDYAIVSTRKDGRPKYNESEGMSRAEIIWQRFQIEKDKMEKNDDRRHLFL
                     LRKGYRILGLGRYQWVDGNINSDWLIATKPKSSGMMPIYSFNERGSGIFCVVSTNKYG
                     DKKAVNLGRDIGFLDYNQRIDLNKLKGSDELDKILTSKSTKG"
     gene            complement(9985..11424)
                     /locus_tag="PKZ29_00375"
     CDS             complement(9985..11424)
                     /locus_tag="PKZ29_00375"
                     /inference="COORDINATES: protein motif:HMM:NF024724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOG37358.1"
                     /translation="MGLDLSFSENYWEDLEYVVKQIPGLEVKEIRRAYEFMCSAHGGQ
                     VRRSGKPYISHPAWIAKVVSQLNIGQEAVIAALLHDCVEDTKITMDDISKEFGDEVAL
                     LVSGLTEVKSRTSKMDASKASIEIYRKFFFSSVNDVRVLIIRLIDKLHNSLTIEYLPK
                     DRQLRYARNIMQVYGPLAEYVGLHYFKKRIEDIAFRILYPEEANKIIKIYENQEKNEI
                     KALNLVKTEVERILKLNNINGAVVEGRIKSLYSTYIKVKRKGGSYRVKDRVAIRILTD
                     NLVDCYTVLGLMHAKYRYIPSEFVDYISTPKPNGYRSIQTTISWKDKMTVEIQIKTKE
                     MHEFNEFGPASHIAYKMGKGEGQGRGMEWVKDLVKWQKTDEHITNYRLKVLTNYVYVF
                     TPKGDTIQLPKGSTALDFAYRIHTNVGDRCKGIKINNKMAKIDDVIQTGDLVEVLLSK
                     KINVNKNWLNIVKTSFAKEHIRKAIKFEN"
     gene            complement(11449..12195)
                     /locus_tag="PKZ29_00380"
     CDS             complement(11449..12195)
                     /locus_tag="PKZ29_00380"
                     /inference="COORDINATES: protein motif:HMM:TIGR01076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0016485 - protein processing [Evidence
                     IEA];
                     GO_process: GO:0045184 - establishment of protein
                     localization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HOG37359.1"
                     /translation="MAVNDFFKVTPEKRQVKIKAKKGVIYSNPTGWKRGYFYLGTGVF
                     LVAIIGLVYLYQPVFNSWINYKEIDQTKVKKETIEIIEKIKTEPPQPTLTAETKPEPE
                     DNTFRVEIPKIGAKADIVIGVSPYDKNEYKAVLKDNVIAQSQISSLPGEGRGTSVYLF
                     AHSSQQDISAARQNSVFYLLGELGNDDMVMINYKGKMFAYRVYMKKVIKPKEIEYLEY
                     KDENKEIVILQTCWPIGTNWQRLLVFAEKV"
     gene            12269..12727
                     /locus_tag="PKZ29_00385"
     CDS             12269..12727
                     /locus_tag="PKZ29_00385"
                     /inference="COORDINATES: protein motif:HMM:NF013440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HOG37360.1"
                     /translation="MTKKNLLTQEGYNSLTQQLKDLKNRQDHLITQIEEVAQPDESGE
                     DGLASQLKEELEVTNNKIDEIEETLADSQIITDKIKTDCIQVGCKVKIKIDSGTKEFH
                     IVNQLESDPTANKISDQSPLGQALIGKKVNDKIEVDAPVGKIIYKIVSIK"
     gene            12756..14234
                     /gene="lysS"
                     /locus_tag="PKZ29_00390"
     CDS             12756..14234
                     /gene="lysS"
                     /locus_tag="PKZ29_00390"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOG37361.1"
                     /translation="MTTDAQTKLNDLQKQRLEQLAKIKSLGIDPYPQPDLSQKSSIAQ
                     ALSLMDKSVEVAGRIMSLRGHGKILFADLHDQSGKIQLFFQEKILGDKMKLTKLLDLG
                     DIVSVCGDVFKTVAGEITINVFKFEVLAKNLRPLPEKWHGLKDVEERYRQRYVDLIVN
                     PEVKQVFQTRTKILSEMRHFLDDHGFLEVETPILQPVYGGAAAKPFVTHHNVLDMDMY
                     LRISDELYLKRLIVGGFEKVYEVSRDFRNEGVSRFHNPEFTQIEFYWAYVDYQVLMNF
                     TQDLIKHVVKSVTGGLKITYQGTEIDFSKPIKKLTLADAIKQHTGIDIDTINTEDEFK
                     SIIKSKKLKLDLTEVVGLGACFDQLYKEYVRPKIIEPTFMCDYPSEMIALAKRHPHDP
                     KKIASFQLVVCGTELLKAYNEVNDPKDQSDRWLSEVELAKKGSEIASQFDHDYIRALE
                     YGMPPTAGWGMGIDRFTQFITDQPTIKDVILFPSMRSEIRDK"
     gene            14224..14577
                     /locus_tag="PKZ29_00395"
     CDS             14224..14577
                     /locus_tag="PKZ29_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic pyrophosphatase"
                     /protein_id="HOG37362.1"
                     /translation="MTSKSLILAKSFLNQTVEVTVDRPLGSIHPKWKFKYLVNYGFLK
                     DIFAPDGEELDAYILKIDKPLKNFSGQVIAIIHRLEDDDDKLVVIPKNETITDQEIEK
                     SVEFQEKWFKHKIIR"
     gene            14584..15132
                     /locus_tag="PKZ29_00400"
     CDS             14584..15132
                     /locus_tag="PKZ29_00400"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOG37363.1"
                     /translation="MKTLTKFISKSGKEIYFRPPTIKDVLALKNHINQISAEKSFLLF
                     QGVQQTTKSETSWLKNKLDKISQQKCVYLCAFFKNKLIGASEVTLHDDAKSHVGNFGI
                     AINKNFRGEGIGSKLMELVIKESIKNIKGLKIIELEVFSGNTNGINLYRKFNFVQFGC
                     LPQGLKRRGHYEDAILMYRKVK"
     gene            15218..16486
                     /gene="serS"
                     /locus_tag="PKZ29_00405"
     CDS             15218..16486
                     /gene="serS"
                     /locus_tag="PKZ29_00405"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOG37364.1"
                     /translation="MIDINFLRQNPDAVRQALVNRQKDSSVVDSIISLDTSRRQLLSE
                     VEKLRSNQNTLNRQFQGKPTPDQIKQAAAIKDNLKQIETQLKSVEEEQNKIIEEIPNI
                     PADDVPVGKDDTENVVIKTVGQIPKFDFKPLDHADLGEKLDVIDVKKAAQVSGSRFGY
                     YKGQGAVLEMAVMFYAFKKLIKKGFVGMIPPTMIKSSTEWKCGYASNKNLFNAYYSSP
                     EDDLVFISSSEHSVVPYHMDEIIPANQLPLKYVNFSPCFRREAGTYGKDMKGMLRVHF
                     FNKVEMNVFTLPDFKISDDMCLEMLSNAEEIMQDLELPYQVVKSCTGDLPQPNRRMYD
                     INSWFPGQEAYRETQSCSNCGDYQARRLNTKVKIDGKNELVHILNSTLVTDRAVLAIL
                     ENHQQSDGSVLIPKALQPYTDFSIIKPKQS"
     gene            16557..>18030
                     /locus_tag="PKZ29_00410"
     CDS             16557..>18030
                     /locus_tag="PKZ29_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003543785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOG37365.1"
                     /translation="MLDFLKNIFDDNSKRIKSYQKIINQINNLELEISKLNDEELSQK
                     TDFFKKQLKKGQSLDDILPQAFAVVREAVKRTIGERAYDVQLMSAITLHQGAISEQRT
                     GEGKTHSVIFPAYLNALTGKGVHIVTPNDYLTRVGTGWYPQALNLLGVSTSCIVHEQS
                     FILDPEYTDETEKIDDRLAHLRPVSRREAYLADVTYGTNNEFGFDYLRDHMAPHQDQV
                     VQRPHHFAIVDEVDFVLIDESRTPLIISSPNNQPTDKYYKFAQIAADLQPTDYVIDEK
                     SRSATLSEYGIRKVERILNVHNLYEESFETIHYIENAIKARALFHKDKDYVVKDGEVI
                     IVDEFTGRLMNGRRWSDGLHQAVEAKEGVTVQRESQTWATITFQNYFRLYQKLAGMTG
                     TAATEAEEFKKIYNLDVVSIPTNKPLQRIDQHDLIFKSQRSKYAAIVNSVEEKYRLGQ
                     PVLIGTTSIAKNEIISSLLRKKGLPHQVLNAKNHRSEAEII"
BASE COUNT         5376 a         4286 c         2927 g         5441 t
ORIGIN      
        1 ctcctattcc cacttcttac cccaccacca ttcctactga cacccccatt cctaccatta
       61 ttcccacctc gactccaatt cccaccaaca cccccgcacc cactaatcct cctcaaccag
      121 ttattagatc taccagcacg cctattccta ctatctatcc tgaagtttta catcttagcg
      181 cctatagagt cgaactctat cccggagagc aacaggaaat caaagctact attattccct
      241 caggtgtttt taataatacc gttacttgga cggttgaaaa cagccacgtc gcccgaattg
      301 aaagtgccaa aaatagattg attattactg ccgccactcc tggcaccacc aaaattattg
      361 ccaaaactat taatgacaaa actgccgaaa ttttaattac catcaaagac accagaactc
      421 aacccacccc atctgtctcc aatgttaccc tcacatctcc aaccgccgcc gcctctaccc
      481 ctattcctac cagagctatc atcgaaccgt catctattag tattaatcaa ccccagcttt
      541 ctctcgaaac caatcgcact attcaactaa ctgtcaccta tacccctgac actaccaaca
      601 gacgtcacct taactggtct agcagtaata ttagtgtcgc tactgttgat gttgccggca
      661 gagttactgc cgttaatcct ggcactgcta ccattactgc ctccagtgtt aatggtcaga
      721 ccagccgttc taccatcacc gtccctggta ctaatttacc tcttcccact tctaccaaaa
      781 ctcctacctc tgttcccact agaactccca ccaatattcc tactcaaatt cccactagga
      841 ctcccactag aattcccact cctattccta ctaccgccat tattaatcct acaggcatta
      901 atcttagcgc tacttctgtc aagctctatt ctggtgaaat tcaaaagata tcagcttcgg
      961 ttgttcctgc caatgctgcc aataaatcac ttcaatggtc tagtaacaat cctaccattg
     1021 ctaccgtcaa cagcttaggt tatattaccg ccaaaactcc cggaactact accgttgtgg
     1081 tcaaaacctc aaacaatatc accaaatcaa tctcagttga agttaataga attcccgttc
     1141 ccaccattat tcccactctt cctataatca acgacaagca ggggactatt actcatgaat
     1201 catacggcgg tatgcagtat attttgtatt taccttatgg ttttactaaa aacagagact
     1261 ggcccttgtt ggtttatctt catggttggg aaagagaacc aaatataaat aatatgtata
     1321 aacaagggca tattccaaga cttctaagag acggtaataa ctataatgca gttgttgttg
     1381 ctccttttag cacccgatct tggggatatt ctagtaatca gcagcaggcc aaagctatta
     1441 ttgatcatgt tgttagtcaa tacaatatca ataaatcaaa aatatctttg acttgtcata
     1501 gtgatggttg ctggggaggt actgccttgg ttagaaataa ttccactttc ttttcttctt
     1561 tggttgcggt tgcttccagt gctcccagtt ccaattccgc ctccggtttt acttcaacta
     1621 aaatctatta ttggtatggt actagtgacg ctgactctac caatcgaagc gctttcgtta
     1681 attccatcaa tcaagctggc ggtcaagctt atatcaaatc tatcgctggc ggacacaata
     1741 ttatgaaaca ggtctatatt gacaataaaa tgattgaatg gatgcttagt caatcaagat
     1801 aacctttcct tctcacccca ataaaaccca aacatttttg ttaaaataca tgatatgtct
     1861 tttaacctcc agtcagacta tcagccctca ggtgatcagc cccgagctat cgccaaactt
     1921 tctcacggaa tcaactctga tatcgctcat caaactcttt taggagttac cggatcggga
     1981 aaaaccttta ctatcgccaa tgttattcaa aacacccaac tgcccacctt agttatttcc
     2041 cacaataaaa ctcttgctgg tcaactctat caggaattta aggaattatt tcctgccaac
     2101 aaagtctctt attttgtttc ttattatgat tattatcaac ccgaagccta tcttccggct
     2161 tcagatactt atattgccaa agaggttgat attaatgaca aaattgatcg tctccggctt
     2221 gaggctacct caaacctttt gtccggcagc gacaatattg tcgtcgcttc cgtcagttgt
     2281 atttataata tcggcgatcc caacgaattt gaagccttaa ctttattatt acaagtcggc
     2341 caaaattatc cccgccgaca gttaatggaa aattttattt ctttgtttta cacccgatca
     2401 gaattagaat ttaaacgttc tacttttcgt gttcgcggcg atcttatcga actttggccg
     2461 tcatataccg actctttttt ggttttagag tttaatggta actatcttgc cgctattact
     2521 caacgccacc cttttaccgc caccgaaaaa aagtttgacc agtttaaact ttttcctgcc
     2581 aaacagtatg tcggcggcta tcaaaattta gataatattt ttgcccaaat tcgccacgac
     2641 cgagaccagc aggtcactat gttcaaatct caaaataaaa ttctcgaagc tcagcggatc
     2701 aatcagcggg ttgaatatga tttagaaatg cttcaggaaa ccggctatat caacggtatc
     2761 gaaaattatt ctatttattt tgacaaaaat cgtcagcacg gcgatccgcc ttataccttg
     2821 gttgattatt ttcatcgtct ttggggcaaa aattttttga ctgttattga tgaatcacat
     2881 gttactgttc ctcaaatcgg cggtatgtat gccggcgatc tcgcccgcaa gcaaaatcta
     2941 attaactttg gttttcgact gccatcggct ctagacaacc ggcctttaaa atttgatgaa
     3001 tttgccgacc ggatctcaaa agtaatttat gtctctgcta ccccggccgt ttatgaactg
     3061 gaaaaatcca aacatcatat tgtggagcag attattcgcc ccaccggttt ggttgatccc
     3121 gaaattagtg ttcgctccac caacaatcag attcccaatc tggttgagga aattcagcgt
     3181 cgggttgaca aaaaagaacg aaccttagtt attactctga ccaaaagaat ggccgaagat
     3241 ctgtcttcct atttgtccga ttctgccaaa accggacata attttaaagt tgcctatctt
     3301 cattctgata ttgttactct tgagcgttcc agtattttag acaatcttcg ttccggtgat
     3361 tatgatgtct tagttggtat caatcttctc cgtgagggtc ttgatttacc tgaagttagt
     3421 ttggtggcta tccttgatgc tgaccaacaa ggttttctcc gctcccaatc atcactcatc
     3481 caaattatgg gtcgggcttc ccgccatatt tccggccacg ttatcctgta tgccgacacc
     3541 atgtctccgg ctatgactgg cgccatcaaa gaggttaacc gccgtcgaca aattcaactt
     3601 aaatacaatc aagatcatca tattactccc aaatcagtgg taaaatcaat tcgtcctcaa
     3661 attattgaag ccgtcgtgcc ggaagaaaaa ccggagtggt tcaaaattga cgtttcttct
     3721 ctcactccca atcaacgtca aaaacaaatc cgggagttaa gaaagcagat gagaaattta
     3781 gctttcaacc tcgactttga agaagccatt aaaattagag ataaaattcg tgagattgaa
     3841 actttttaat ttcactattt atatatgatc gacaaaataa ttgttaaagg agccagagaa
     3901 aacaatctta aagatgtttc ttttgagttt cccaaaaata aattagttgt ttttaccggc
     3961 gtttccggct ccggcaaatc aagcatggct tttgacactc tttttgccga aggtcaacgc
     4021 cgttatgtcg aaagcttgtc tgcctatgcc cgtcaatttt tgggcaacat gaagcgtccc
     4081 gaagttgacc tgattgacgg cttatcgcca tcaattgcca tcaatcaaaa agccatttct
     4141 cacaaccctc ggtctactgt tggcactatc accgaaatct atgactatct ccgtttgctt
     4201 tttgcccgta ttggtcatcc ccactgtcct atttgtgatc aggaagtagc cccccaaacc
     4261 agccgtcaaa ttgtttccca aattatcacc atcgcccaac atcaggttca aggtcttaat
     4321 tattatcggt ttttgattct ctcacctgtc gtccgtgaca aaaaagggga ttttaaagga
     4381 ctaaccgata acctcaaaaa acagggctac aaatggctcc gagttgacaa tcaaattatc
     4441 gatttatact ctgattttgg tattgccaag accaataaac ataatattga tgtggttata
     4501 gaccgtattt ctatcaacaa agacattctc aaagaccaaa aacctctcac cgatcgtctg
     4561 attgacagtg ttgagcaggg gatgaaacta gccaacggtt tagtgatcat ttccgaagtc
     4621 ttaagctcat cttttgattt tcccgatttt cccaaagatt tccacgatac tctttactcc
     4681 gaaaattttg cttgtcctaa ttgtaatatc tctttgccag aaattgaacc ccggcttttt
     4741 tcctttaatt ctccccaagg cgcttgtccc gaatgcaaag ggttggggtc taaatatcaa
     4801 attgaccgtt ccaaatttcc cgactggcgc gcccgaatga tggaaagccg ctacgtcaat
     4861 tccggttctg acgctattcg cgatgagctg gaaaaattta tgatcaaaac cgaatgtcag
     4921 ctttgccatg gtagccgtct aaatcaggac gccctttcta ttcatatttt aagcaaaaat
     4981 atttaccaaa ttacccaata tcctatcgaa aaacttagtc tgtggatcaa cactttaaac
     5041 tcaaaattga ccagtgatca ggaaaaagaa attttaattc ccatcaaaca ggaacttacc
     5101 gccaggattg agttcctttt ggctgtcggt ttagattatc tcagtctcga acgtgaagcc
     5161 ggcaccctat ccagcggcga atcgcagcga atccgtctag ccagtcaaat cggcactggc
     5221 ctgaccgggg ttttgtatat tcttgacgaa cccactattg gtcttcattc ccgtgacaac
     5281 aatcgtttga ttgaaactct caaaaaactt cgggataccg gcaacaccct ggtcgttgtc
     5341 gaacacgacg aagatgttat caaaagtgcc gaccatattg tcgattttgg actttatgcc
     5401 ggcaaaaaag gcggtcaaat tgtcgcccaa ggctcaattg acgacatcaa aaaaagtccc
     5461 caatctctta ccggcaaata tctctccggc aagctcaaga taaaatcatc ccaaaacccg
     5521 gtttctcaac aattagcatc aaacagaatc tccattacca acgccagaca gtggaatctt
     5581 aaaaatatca atgtcgattt tcctctcaac aaattggttt gtgttaccgg cgtttccggc
     5641 tccggcaagt caactctagt ccatgacact ctttatggcg gtgtccgcaa agccattttg
     5701 ggcagctatt acggcaccat cggtaaattt gaccaaatca ctggcgccga atccgtttcc
     5761 gatgtccttt tggtcgacca atctcccatc ggtcggactc cccgcagcaa tcccgccacc
     5821 tacaccaaaa tttttgatga catccgtcaa ttaatgtccc aaaccattga agctcagctt
     5881 cgcggttacg acgccagtcg tttctctttc aatgtcaaaa acggccgttg ccctgtttgt
     5941 gaaggtcagg gtcaaatcaa aattgagatg cagtttttgc ccgatattta cgttacctgt
     6001 gatgaatgcc acggcagccg cttcaaagcc gaaactttgg aagtcgaata caaaggcaaa
     6061 aatatttccc aaattctcaa cctaaccatc gacgaagccg ccgatttttt caaaaatatt
     6121 cccaaactaa aacgtaattt agaaaccatc caatcagtcg gtctgggcta tttggagctg
     6181 ggtcaatcgt ccaatactct ttccggcggc gaatcacaac gtcttaaaat cagtcgtgaa
     6241 ttagtcaaaa aagaaaatgg tcgcatgctt tatattttag acgaacctac caccggactt
     6301 catttttatg acgttgataa attaattacc gttctccgcc gtttagttga caagggtaat
     6361 actgtcgtgg ttattgaaca caatttagat atgatcaaaa attctgactg gattattgat
     6421 atgggtcccg aaggagggga taagggtggt caagttgttg ccgtcggcac tgttgaagat
     6481 attgttaatt gcaccaaaag ttacaccggt caatatctta aaacccatct ttaaacttat
     6541 ttacactttt ctctgatttc taccattgtc ttccaattcg tttccaactt atcagtcaca
     6601 caatagtctt taaactgatc cagccaaccc acgtagtcat ttttaacttc ataaactaaa
     6661 gctattttag atttatcatt ctttttaacc atcctataaa actcatcctg agatcggaca
     6721 tattctggca cttgggctaa actataagac actttttgcg gcatataacc atattttttt
     6781 accccatacc accaatacaa atattggttt ggataatcat aaatctccgg cacataagtg
     6841 taagcttcaa atccgtctcc atttattttt tcgtataccc agtcaatatt tcggattaaa
     6901 ttagccaaat tttttggatc atcgcttcct gttttgttca taaatttgga ctggtcgact
     6961 acggttagcc aaaaattcct tatcaaaaac acgcccaata ttattccgat tattttttga
     7021 tatttactta tatttactat tcctatcccc actaccagta cataccacac ccttaaactt
     7081 tccagatacc acatttttaa cggatgatga tataccgcta tataaaaccc taaagccaac
     7141 agccaaaata ccaaaaaata caacccatag cgccgatatt ttttgtttgc caaaatcaac
     7201 cccacggcta ccaaaaacac tattattccc caaccataag gcaaaattag ctgtccttca
     7261 aaaaaataaa tcatagactt cagatggcta gaaactgttt ctgttagtag caacttttct
     7321 cctaatacct gattttgacc gctaatcatc cccaacaaca atttcgtcat ctgaaatcta
     7381 ttttttattt ccaaagcaat ttgtggcaaa aaccagggta ttaatcctat caaaaaattt
     7441 ttccaccaac ctaatttttt atttatcact attatcacaa ccgcaaacaa aatacacacc
     7501 accccaaaag ccgcctcaaa ttgagtcagt aaaccacttg ttattcccag ccaaaatatc
     7561 gactttttgt cttttttttc caaaaaattc cataccgcca aataaaaata gacgctcaaa
     7621 tatatcgcca tattagcatt ccaaaaatat cttgtcacat taaacatctg tggcgccatc
     7681 aaaaacaaac tgccaacaat aaaacccaaa actatatttt tctttctgaa aaaataaaaa
     7741 ataaaaccgg cagttgccaa aaacaaaata atgttccaat taactagtgt ttgtggattg
     7801 ccgccacccg cccaataagc cggcaaattt atgtagtaat aaaacggacc caatcttaag
     7861 ccgttaatag aagttgttgg ccccaatacc gccagatccc taaactctcc caaagctctg
     7921 atatccaaca aatcgcgtga ttgatcatac gtaaaagcgg tttgataatt ccacacctgg
     7981 ccaaacctaa ttactgccga tatcacccat attatgccga acactaatga taaccatttt
     8041 tcttttgctg tcatgaattc attatataat accaatgtga ttgatttaac cactattccc
     8101 agctctccag gagtttatat ctaccaaaat caacaaaaag aaataattta tgttggcaaa
     8161 gccgttaatc tcaaaaaacg ggtgttacaa tattttcaaa atcgagatgc tctcggttac
     8221 aagacccgcc agcttgtttc tcaaattgct tccgccaaat ttattaccgt cgactccgaa
     8281 atcgaagctc tagttttgga gtccggtctt atcaaacaat accgtcccaa atataactct
     8341 caactcaaag atgacaaaag ttatatttat atcaccattt cccgtgataa atttccttta
     8401 gttcgacctg ccttcaaatc ttctcttaat gacaatgata ttatttacgg tccttttccc
     8461 gacagccgct ctgtcaaaag tttgctcaaa accattcgcc aaatatttcc ttactattcc
     8521 gccaaacatg gtctcaagtc ctgtctcttt tgtcatctgg ggctttgtcc tggacccgcc
     8581 cccgacccca aagaatacaa agccaacatc agcaaaatca aaaaaattct taccggtcat
     8641 ttccacaaac ttatcaacca actaaaaacc gagatgaaac ttttttccca agccgaaaat
     8701 tttgaaatgg ccaaagtccg ccgtgatcaa attaactctt tggaatatat tatttccggt
     8761 tggagccggc tgactcatct ctatcaacaa gttgatttac ccgaagataa agccagcaaa
     8821 gctattgctg aattaattct ggttctaaaa gattattttc cccaattaac atctttaaac
     8881 cggattgagg cttacgatat ttccaattta ggttccaaat attttgtcgg ttccatggtg
     8941 gtatggcagc agggaagaat tgatcacgac caattccgcc aatttaaaat ttattccaaa
     9001 gtcactcctg acgatcaatt tatgatcaag gaagttttat accgccgttt caaacacccc
     9061 gaatggcagt ttcccggttt gattttggtt gacggcggca aaccccaagt ctccgccgcc
     9121 accaagttaa ttgatgaact tgaaaaactt tataacttta aattatctat aattggtttg
     9181 gccaaaaaac aggaaactat tgttatcaaa accaatattg attgggtgga aataaatctg
     9241 cccaaaaatt ctcccgctct tcaactactt caacaactcc gcgacgaggc tcatcgtttt
     9301 gccaaccatt atcgccgtca gctaatcaga cgctcaaccc ttagttgact tgctagtaag
     9361 tattttatcc aactcgtctg aacctttcaa tttattcaaa tcaattcttt gattataatc
     9421 aagaaatccg atatctctac ctaaattaac agccttttta tcaccatatt tgttggtgct
     9481 gactacacaa aaaatcccgc ttccgcgttc attaaaagaa tatatgggca tcatgccact
     9541 tgatttaggt ttagtggcta tcaaccaatc actgtttata ttgccatcca cccactggta
     9601 tctacccaat cccaaaattc tgtatccttt tctaagcaaa aaaaggtgtc tgcgatcatc
     9661 atttttctcc attttatctt tttcaatttg aaatctttgc cagataatct cggcccgact
     9721 cattccctca ctttcattat atttaggccg accatctttt ctagtgctga caatggcata
     9781 atctctcatt tctgtcgttg gctgaccatt ttcaaacact aagccgattt gtctagctcc
     9841 cgtatctgtt gccggcatag ctgttaagtc agatctatgc ggcagttcat tagccggcaa
     9901 tccgtatgca gttggattta agccttcagc tgttttgctc ataattattt atcctataac
     9961 actataatta tatcaaaaaa taatttaatt ttcaaatttt atagcttttc taatatgttc
    10021 tttagcaaaa ctggtcttaa ctatatttaa ccaattttta ttaacattta tttttttact
    10081 caacagcact tctactagat caccggtctg aattacatca tcaatcttgg ccattttatt
    10141 gttaattttt atccccttgc atctatcgcc gacattggtg tgaattctgt aggcaaaatc
    10201 caacgccgtg ctgccttttg gtagctgaat tgtatcgcct tttggggtaa acacatagac
    10261 ataattcgtc agcaccttta atcgataatt agtaatatgt tcatcggttt tttgccattt
    10321 aaccaaatct ttcacccact ccattccccg accttgacct tctccttttc ccattttgta
    10381 ggcaatatgg gatgctggtc caaattcatt aaactcatgc atctctttgg tttttatctg
    10441 gatttccact gtcattttgt ctttccagct gatagttgtc tgaatcgatc gatacccgtt
    10501 gggtttaggt gtcgaaatat aatcgacaaa ttcgcttggt atatacctat atttagcgtg
    10561 catcaatccc aatactgtat aacaatcaac caaattgtcc gtcaaaattc taatagccac
    10621 tctatctttg acccgatagc ttccgccctt tcgttttact ttgatataag tgctataaag
    10681 gctttttatc cttccttcca ccactgcccc gttaatatta tttaatttta aaatcctttc
    10741 aacttcagtt ttaaccaaat ttagggcctt aatttcattt ttttcctgat tttcatatat
    10801 ctttattatt ttattagctt cttccgggta gagaattcta aaagcaatat cttcaattct
    10861 ttttttgaaa taatgaagcc cgacatattc tgctaacgga ccatagacct gcataatatt
    10921 tctggcatat cttagctgtc tatcttttgg taagtattca attgttaaac tgttgtgtaa
    10981 cttatcaatt aatctgataa tcaatactcg gacatcatta accgacgaaa agaaaaattt
    11041 tctataaatc tcgatactag ctttgctggc atccatctta ctggttctgc tttttacctc
    11101 agtcaaacct gataccaata aagccacttc atcaccaaat tctttggaaa tatcatccat
    11161 ggtaattttg gtatcttcca cacaatcatg aagtagcgcc gcgatcaccg cttcctgacc
    11221 gatgtttaat tgagacacta ctttagcaat ccaggctggg tgggaaatat atggtttacc
    11281 cgaccgtctt acttgaccgc cgtgagctga gcacataaat tcataagccc gcctaatttc
    11341 cttcacttcc aaacccggaa tttgtttaac tacatattcc agatcttccc aataattttc
    11401 actaaatgac aaatctaatc ccatataaag ataaattata gcaaaaaatt aaactttttc
    11461 tgcaaacacc aacaatcttt gccagttagt gcctatcggc caacaagtct gcagaattac
    11521 aatttcctta ttttcgtcct tgtattcaag atactctatt tccttgggtt taatcacctt
    11581 tttcatatat accctgtagg caaacatttt tcctttgtaa tttatcatta ccatatcatc
    11641 gtttcccaac tctcccaaca gataaaacac cgaattttgt ctggcggcag aaatatcttg
    11701 ctgactggaa tgggcaaaca aataaaccga tgttcccctg ccttcacccg gcagagatga
    11761 aatctgtgat tgagcaataa cattatcttt taatactgct ttatattcgt ttttgtcata
    11821 aggagatact cctataacaa tatctgcctt tgccccgatt tttggtatct ctaccctaaa
    11881 agtattatct tccggctccg gtttggtctc tgctgtcaaa gttggttgtg gcggttcagt
    11941 ttttattttt tcaattattt ctatcgtttc ttttttaacc ttagtctgat caatctcttt
    12001 gtagtttatc cagctgttaa acaccggctg gtataaataa accagaccta taattgctac
    12061 caaaaacact ccagttccca aataaaaata acctcttttc caccctgtcg gattgctgta
    12121 aattactcct tttttggcct ttatcttgac ctgtcgtttt tccggtgtta ctttaaaaaa
    12181 gtcgtttact gccatagatt attatggcat aaatttaatt tgacaaaaga tagttattac
    12241 tttaagatac aaactcattg acacaaatat gaccaaaaaa aatctcttaa ctcaagaagg
    12301 atacaatagc ctgactcaac aactcaaaga cctaaaaaat cgtcaggatc atcttattac
    12361 tcaaatcgaa gaagtagctc aacccgatga atcgggtgaa gacggtctgg cttcccagct
    12421 caaagaagaa ctcgaagtca ccaacaacaa aatcgacgaa atcgaagaaa ctttggctga
    12481 tagtcaaata attaccgaca aaatcaaaac agactgtatt caagtcggct gcaaggtaaa
    12541 aatcaaaatt gactccggca ccaaagaatt tcacatagtt aatcaattgg aatctgatcc
    12601 gacagccaac aaaatctctg atcagtcgcc tctgggtcaa gccttgattg gcaaaaaagt
    12661 taatgacaaa attgaagtcg atgctccggt cggcaaaatt atctacaaaa ttgtttctat
    12721 caaataattt cccttgatta tagtaaaata ggggcatgac taccgacgcc caaaccaagt
    12781 taaacgatct ccaaaaacag cgcttagaac aactggccaa aattaaatcc ctggggattg
    12841 atccttatcc ccaacccgac ctgtctcaaa aatcttccat tgcccaagct ctaagcttaa
    12901 tggacaaatc ggtcgaagtt gccggtcgaa ttatgtctct tcgcggtcat ggcaagattc
    12961 tttttgccga tcttcatgac caaagcggca aaattcagct ctttttccaa gagaaaattc
    13021 ttggcgacaa gatgaaactt actaaactgc tagatttggg cgatattgtt tctgtttgcg
    13081 gcgatgtttt taaaaccgtt gccggtgaaa tcaccatcaa tgtctttaaa tttgaagttt
    13141 tagccaaaaa tctccgtcca cttccggaaa aatggcatgg actcaaagat gttgaagaac
    13201 gctatcgtca gcgttatgtt gatttaattg tcaaccccga agtcaaacaa gtttttcaaa
    13261 ccagaaccaa aatcctttcc gaaatgagac actttttgga tgatcatggt tttctcgaag
    13321 tagaaacgcc tattttgcag ccggtttatg gcggcgctgc tgccaaacct tttgtcactc
    13381 atcacaatgt tttggatatg gatatgtatc tgcggatttc cgacgaactt tatctcaaac
    13441 ggctgattgt cggcggtttt gaaaaagttt atgaagtcag ccgcgacttt cgcaacgagg
    13501 gtgtttcccg gtttcacaat cctgagttta cccaaatcga gttttattgg gcttacgttg
    13561 actaccaggt tctgatgaac tttacccagg atctaatcaa acatgtagtt aaatcagtca
    13621 ccggcggtct caaaataact tatcagggca ccgaaattga ttttagcaaa cccatcaaaa
    13681 aactaactct tgccgacgcc atcaaacaac acactggcat tgatattgac accattaata
    13741 ctgaagacga atttaaatca attatcaaaa gcaaaaaatt gaagcttgat ctgaccgaag
    13801 ttgttggttt gggcgcttgt tttgaccaac tttacaaaga atatgttcgt cccaagatta
    13861 ttgaacccac ctttatgtgt gactatcctt cagaaatgat tgctctggcc aaaaggcatc
    13921 ctcacgatcc caaaaaaatc gcctctttcc aattggtggt ttgcggcacc gaacttctca
    13981 aagcctacaa cgaagtcaac gatcccaaag atcagtctga tcgctggctt tccgaagttg
    14041 agttggccaa gaaaggttct gaaattgcct cccaatttga tcatgattat attcgggcgc
    14101 ttgagtatgg tatgcctccc accgccggtt ggggcatggg aattgaccgc tttacccagt
    14161 ttattaccga ccaacccact atcaaagatg ttattctttt cccctctatg aggtcagaaa
    14221 tccgtgacaa gtaagtcttt aatcttagcc aaaagttttt taaatcaaac cgttgaagtt
    14281 actgttgatc gtccacttgg cagtattcat ccaaaatgga aattcaaata tctagttaat
    14341 tatggttttc tcaaagatat ttttgcccca gacggcgagg aattagatgc ttatattctc
    14401 aaaatagaca aacccttaaa aaatttttcc ggccaagtta ttgccattat tcatcggctt
    14461 gaggacgacg atgacaaatt agtagttatt cctaaaaacg aaactattac cgaccaagaa
    14521 atcgagaaat cagttgaatt tcaagaaaaa tggttcaaac ataaaattat tagataattg
    14581 attatgaaaa ctttaactaa attcatttcc aaatccggca aagaaattta ttttcggccg
    14641 cccaccatca aagatgttct tgctctcaaa aaccatatca accaaatttc ggcagaaaaa
    14701 agttttcttt tatttcaagg ggttcagcaa actaccaaat cagagacctc ttggctcaaa
    14761 aataaattag acaaaatttc ccagcaaaaa tgtgtttacc tttgtgcttt tttcaaaaat
    14821 aaacttatcg gcgcctccga agtcacttta catgacgatg ccaaaagtca tgtcggcaac
    14881 ttcggcattg ccataaacaa aaatttccga ggcgagggga ttggtagcaa attaatggaa
    14941 ttagtcatca aagaatcaat caaaaatatc aaaggtctaa aaataattga actagaagtt
    15001 tttagcggca ataccaacgg cataaactta tatcgaaagt ttaattttgt tcagtttggt
    15061 tgtctgcctc agggtctcaa acgtcgcggt cactatgaag acgctatttt gatgtataga
    15121 aaggtaaaat aagtattgat atattttgat atattaacta actacggtta atctgcgggt
    15181 attatttaat cttaggtttg gtaaaatact tccatcaatg attgatatca attttcttag
    15241 acaaaatccc gacgctgtcc gccaagctct tgttaaccgc caaaaagatt cttctgtagt
    15301 tgattccatt atttctttgg acaccagccg ccgccaactt ttgtccgagg tcgaaaagct
    15361 cagatcaaac caaaacactc tcaatcgcca gtttcagggc aagcctactc ccgaccaaat
    15421 caaacaagct gccgccatca aagacaatct caaacaaatc gaaacccaac tcaaaagcgt
    15481 cgaagaagaa caaaataaaa ttatcgaaga aattcccaat attcccgccg atgatgttcc
    15541 agttggcaaa gacgataccg aaaatgtggt tatcaaaact gtcggtcaaa ttcccaaatt
    15601 tgattttaaa cctctcgatc acgctgattt gggcgaaaaa cttgatgtta ttgacgtcaa
    15661 aaaagcggct caagtttccg gctcccgttt tggttattac aaaggtcaag gagccgtgtt
    15721 ggaaatggcg gtcatgtttt acgccttcaa aaaattaatc aaaaaaggtt ttgtcggtat
    15781 gatcccgccc actatgatca aaagttctac tgagtggaaa tgtggctatg ctagcaacaa
    15841 aaatttattt aatgcttatt attcctctcc cgaagacgat ctcgtgttta tcagcagttc
    15901 cgaacactca gttgtgccct accatatgga cgaaattatc ccagccaatc aattgcctct
    15961 aaagtatgtt aatttttctc cctgttttcg gcgcgaagcc ggcacctacg gcaaagatat
    16021 gaaaggtatg ctccgggttc acttttttaa caaagtagaa atgaatgttt ttactctgcc
    16081 ggatttcaaa atttctgatg atatgtgtct ggaaatgcta tcaaatgccg aagagatcat
    16141 gcaggatctg gaacttcctt atcaagtcgt caagagctgc actggcgact tgccccagcc
    16201 caaccgtcgc atgtatgaca tcaattcttg gttccccggt caggaagcct atcgcgaaac
    16261 ccaatcatgc agcaactgcg gcgactacca agcccgccgt ctcaatacca aagtcaaaat
    16321 tgacggcaaa aacgagcttg ttcatattct taattccacc ctcgttaccg acagagctgt
    16381 tttggcgatt cttgaaaacc atcaacaatc tgatggcagt gtccttatcc ccaaagccct
    16441 ccaaccctac actgattttt ccattataaa acccaaacaa agctagagga gccacccttg
    16501 tccggttcct tcatcaagaa ggaatcggac agaggttttt ttgataaact aaacctatgc
    16561 ttgatttcct aaaaaatatt tttgacgaca attccaaacg gataaagtct tatcaaaaaa
    16621 taatcaatca gatcaacaat ttagaactgg aaatttctaa gttaaacgac gaagaattat
    16681 cccaaaaaac tgatttcttc aaaaaacagc tcaaaaaagg tcaatcatta gacgatattt
    16741 taccccaagc ttttgccgtt gttcgcgaag ccgtcaaacg caccatcggc gagcgggctt
    16801 atgatgtcca gctgatgtca gccatcactc tccatcaggg ggctatttcc gaacagcgca
    16861 ccggtgaggg taaaactcac tctgtcattt ttcccgctta tcttaatgct cttactggta
    16921 aaggcgtcca tattgttacc cctaacgatt atcttacccg ggttggtacc ggctggtatc
    16981 ctcaagctct caatttgttg ggcgtctcca cctcctgtat tgtccacgaa cagtcattta
    17041 tccttgaccc tgaatatacc gacgaaaccg aaaaaattga tgaccgtcta gcacatctcc
    17101 gtcccgtctc ccgccgggaa gcctatctgg ctgacgttac ctatggcacc aacaacgaat
    17161 ttggtttcga ttatcttcgc gatcatatgg ctccgcacca ggatcaagtc gttcagcggc
    17221 cgcatcattt tgccattgtt gacgaagttg attttgtttt gattgacgaa tcccgcactc
    17281 ctttaattat ttcttctccc aacaaccagc ctaccgacaa atactacaaa tttgcccaaa
    17341 ttgccgccga tcttcaaccg acagattatg ttatcgacga aaaatcccgc tccgccactc
    17401 tctcagaata cggtatccgc aaggtcgaaa gaattctcaa cgtccacaat ctttatgagg
    17461 aatcgtttga aactattcat tatattgaaa atgccatcaa agcccgggct ctgtttcata
    17521 aagacaaaga ttatgtcgtc aaagacggtg aagtcattat tgtcgatgaa tttaccggcc
    17581 ggctgatgaa cggccgtcgt tggtccgatg gacttcatca agcggttgag gccaaagaag
    17641 gtgttaccgt ccagcgtgaa tcccaaacct gggccaccat tactttccaa aattattttc
    17701 gcctttacca aaaacttgcc ggtatgaccg gcactgccgc caccgaggct gaagaattca
    17761 aaaaaattta taatctcgat gttgtttcta tccccaccaa caaaccgctt cagcgtattg
    17821 accagcacga ccttattttc aaatcccaac ggtcaaaata tgctgccatt gtcaactcgg
    17881 ttgaagaaaa atatcgcctg ggtcaaccgg ttttgatcgg cactacttct attgccaaaa
    17941 acgaaattat ctcctccctt cttcgcaaaa aaggtttgcc ccaccaagtt cttaatgcca
    18001 aaaatcaccg ctccgaagcc gaaattattg
//
LOCUS       DAOSHV010000004        16969 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_2746_length_16969_cov_5.070238, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000004 DAOSHV010000000
VERSION     DAOSHV010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 16969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16969
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2746_length_16969_cov_5.070238"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(112..1077)
                     /locus_tag="PKZ29_00415"
     CDS             complement(112..1077)
                     /locus_tag="PKZ29_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HOG37366.1"
                     /translation="MKIGGKPVGLGCPTFVVAEIGINHNGSLPLALESIEVAADIGVD
                     AVKFQKRAVNIVYSPEELATPRDFDREILDAALERQEIEGVRYDVFPPDVLARIMAGE
                     WQTNGVLKHALEFGLKEYDLIDKRCAELGIAWSASPWDGLSAHFINGFSIGWVKIASA
                     CLTNRDLLERVRETGKTVVLSTGGSTIRQIQEAVRILGRENLALMHCLAVYPPADEDT
                     NLLVLETLRRAFPGVPVGFSSHARDTFPVVAAVAMGAELVEAHLTLDRNMSGSDHKAS
                     LEPLEFKNMVSQIRRLETLRGDGIKRVLSPEAKIMEKLRRVDNLF"
     gene            complement(1118..1906)
                     /locus_tag="PKZ29_00420"
     CDS             complement(1118..1906)
                     /locus_tag="PKZ29_00420"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOG37367.1"
                     /translation="MFSLEGMHVLITGAGGFFGQVFVESLCSAGAAMVYALDRDLSWF
                     SVTSSNLKLCQIDLDQELELANLCNSITSQGGVDIIVNNAYDWKAAGFGDQSARLETM
                     SSEGFVGSLKTDWWAVKLAQLFVTGMRARGNGNIINIASMYGLVAPDPSLYEDTDYFN
                     PASYGMAKAAMVALTRYMAAWLGPEIRANALVPGAFPNYGDSDNSKQNQNQQFTERLK
                     RRTLLKRLGVPKTDLVGPLLFLASDASRYMTGQTLVIDGGWTVT"
     gene            complement(2023..2619)
                     /locus_tag="PKZ29_00425"
     CDS             complement(2023..2619)
                     /locus_tag="PKZ29_00425"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HOG37368.1"
                     /translation="MLIKGKKLKEVASRVKAVAMDVDGCLLPDVECLLPAEQVAKFRS
                     RVDGQGVSLMRAIGLSVVFITAEKKGTPGFEPIKRLVDNWNRLPSSQINKGDNHWPMV
                     ELFGGKDTQSKGDILTAWLKEKKIKVEECAVMGDDLVDVPMLKIGGFKVAPVSAENTI
                     KELADWVTDRPGGFGAVRDLANLLVSVKKIDPYSLPCK"
     gene            2742..4328
                     /locus_tag="PKZ29_00430"
     CDS             2742..4328
                     /locus_tag="PKZ29_00430"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG37369.1"
                     /translation="MGTFSILKGLQVALINMPIREGAKPNTPPLGPGLLAAQLRQFGA
                     SVSLIDLNVYRLKDKLAERQSLLNGRHLSLEEATGLISRYFNRDGEPCLIGLSGMITT
                     LKWQSQIASVCRKMVPRACLVSGGGLATEMKGVLFNWIPELDAIVLGEGDKAVIRLAL
                     QVKKGKIKPGERISLTGTRPDLDALSLPAWDLMEQDVDGCPILEHYIRTPVWGGSGTQ
                     NSSAAPFAMERSLTTVSSRGCPYNCAFCFRGAQGARNYGQRSADSLIKEACWLRDRYA
                     IDFLGFNDDNFAVMPDRLNLLATRFPDEVGLHWGTHIRLDMADDRLYTMAKAGCIYAG
                     YGAESASPRVLTLMNKGGMMLTQGLVRVNGFLFPRTMVEGVIKTQEAGIHGNCTWIMG
                     YPGEKLDDLKTSVGFILWQVEKATESLASGTREYQGASEAVNQNMFMATAYPGTAMFR
                     TKPVRERLSRQFGLKFSTKGRVIADSALRLYVESLGDAANVISDKNGNPINFSDISDD
                     KLLIARSLISQGKIGKILNL"
     gene            complement(4363..5325)
                     /locus_tag="PKZ29_00435"
     CDS             complement(4363..5325)
                     /locus_tag="PKZ29_00435"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOG37370.1"
                     /translation="MSNRKPALDYIKWHINDNVKRETDNHYRTKLALKLRDKNPWEII
                     SRLTFDSVAPNLELGEVRELFGWVDRKILNHGLKGVGVELGSGVGFMSSVIASLPSVE
                     KVYAVEIVENIVRDLSPKVIKFVLGNKTEKVTSCVGDFDYLGLADNQADFVFDFFSLH
                     HSPDLVKTLSEVFRVLKPGGFIFCFDKARSDRMSKEDLEKLLDKEYDPQTKILMGLPS
                     RISHTRRQNGEREYRWLDWQQAFSKAGFSSTKHFHLARIAGGWLAHLLKICLTLLPNK
                     WQIIFTNRWYRNKNYNDLELSDLLFCPPVKNFPKEISLMIAYKK"
     gene            5349..6068
                     /locus_tag="PKZ29_00440"
     CDS             5349..6068
                     /locus_tag="PKZ29_00440"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HOG37371.1"
                     /translation="MKILGIIPARGGSKRVPNKNIKMVGGKPLIAWSIESALKSDLDR
                     LVVSTDDEKIARIAKKYGAEVPFLRPGELSGDKLGIEPVLKHALEWLLEKENFKPEAV
                     ALLMATAPMRRPEHINQAIKIFKNKKADSVISVIEAKANSNPHWLLKKDSKDQLVLFT
                     GKPLTKIITRSQDLPVCYSRNDIIYVLKPKNLYDKTPNLYGKKTELLVMDDFYNVDIN
                     TPEDLFICRQKIKLLKKQNIL"
     gene            complement(6075..7454)
                     /locus_tag="PKZ29_00445"
     CDS             complement(6075..7454)
                     /locus_tag="PKZ29_00445"
                     /inference="COORDINATES: protein motif:HMM:NF016357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HOG37372.1"
                     /translation="MNSKKTILITVFHSFISKNILDTDVFKDLRQSDLNILLVVPNPK
                     KNFYEMTYGGDNVCVIGINSTKLLNSKKTKFFSRLAYLLQFSHYLNYKKIERKNAKGN
                     KIIKSIRYCFEVLATGLLAGKRPVRFLMRYFFMLGQSQKEIIGLFQSSSPAIVFSTDI
                     FDESDVLIAREAKKRGIPTVGMIRSWDNCYSKGIMRVVPDWLIVNNETLKEEVVCMHD
                     VNPQDVFIAGLPQFDRFVNEPRSTRQEFCQKVGLDANRPFVVIAPAGKILSDTDWQIA
                     DMLKRAKIDGRLPKIQFLIRNHPNHPADFFNFENDLDFVIENPGKVFNQNNPKETELT
                     SLDSKHLADTLYHSSLLIYTATTLGLDALVFNKPQIIIDFDGYEKKDYWHSVSRYHDE
                     EHMKKMIACGGVRVVSSESELVGTVREYLSDPNLDQMGRNKMLRQQLFKLDGQSGKRI
                     VNFIKKKTR"
     gene            complement(7444..8175)
                     /locus_tag="PKZ29_00450"
     CDS             complement(7444..8175)
                     /locus_tag="PKZ29_00450"
                     /inference="COORDINATES: protein motif:HMM:NF018696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HOG37373.1"
                     /translation="MKFKLFLHKVKNDLKKIIGYPPPDFGKDGTVDYDKYWKKRRPSG
                     FLDVLSSWQKQRADLVLPLLENGNVVVDVGGGSGEMLAYWVKLKKIKGICADYNELAL
                     EQAKRRGLDTLFLDLNDEKNWNDVPPCDYLTGFEILEHLANPEKFILFVKNKVRKGLI
                     FSVPNSGYYQHRVRLLLGRFPLQWIVHPGEHLRFWTAKDIVDWVGFLGLKLEKKIIYE
                     GLPGLNNLLPSLFGQGIIIYIKNEQ"
     gene            complement(8165..9283)
                     /locus_tag="PKZ29_00455"
     CDS             complement(8165..9283)
                     /locus_tag="PKZ29_00455"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOG37374.1"
                     /translation="MRIILFTSELSEKNGWGRYSLELACVYKKFNHEVIVVCNKANNN
                     EAIAQIELLPGPLSYYRNYFLFFLSVIKIVFWIKDGKETVVHCLVETYLPSAWLISKL
                     YSCPLFLTVHGSFGLKPLAFRVVGSIHKKLYQLADKVICVSSYTKDRLITAVGNLGNI
                     EVIPNGVNTTNTQLSSPRAGCGNGRQVLLGVGALKKRKGFHLVLRALPDILKIFPNLE
                     YWIVGSRDDQDYYCKLLDIINENQIGERVKFFYNISDQELDFLYTQACLLVLTPISDQ
                     YNFEGFGLVYLEANLRGLPVVGSLENGGEEAIKDGKTGFLSRPNDPKDIARLIIKILS
                     DQKLYSMMSEEATKWAQNHDWSFIAKRYLNLYKTKDEI"
     gene            complement(9280..10395)
                     /locus_tag="PKZ29_00460"
     CDS             complement(9280..10395)
                     /locus_tag="PKZ29_00460"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOG37375.1"
                     /translation="MRLLYITSARLPTTKAHGYQIAKMSEAFQESGVKLILVAPRRHN
                     PDKKDFSDYYKLRNKVEVIYLPCADWTFIPWHRLRFWLMILTFGLSLRFFILKNKKSQ
                     IYIREPLLALFLKGFFLEVHMLPVKMRFYHRWLFGRAKSFIVLTSFIKRELGLTYPAK
                     KCLVSPDGVDLAEFDIFEKEKVIRQRLGLPIDKKIILYTGSFYLYGWKGVDVILETAK
                     LFGDDVLFVLVGGNDKDLKKIKKENCLKNIQLVGFRPHSDMPYYQKAADVLLLPNKTG
                     DRLSEECTSPLKLFEYMASGRPIVASDLPSIREVLNENNAVLVSPNLAEALKNGVKSV
                     LINSEQANNLAIQAKKDVREHTWLNRAQKIKEFLATL"
     gene            complement(10392..11009)
                     /locus_tag="PKZ29_00465"
     CDS             complement(10392..11009)
                     /locus_tag="PKZ29_00465"
                     /inference="COORDINATES: protein motif:HMM:NF017069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOG37376.1"
                     /translation="MKNLIFRLRYLVGKVFGRDLIFDNYLKNRRVLDVGCGRGEILKK
                     DLQNFFGIDANPTVVNDLQSQGYQARLAEASNLPYPDNQFETVYCSNLIEHLFPDQAY
                     AMLKEANRVLKKHGTIILISPMPMSIWNTFGHIKPYPPLAISKILRPFSLERVDAIDT
                     LVMDKVVYLGHWANNKILFFLTSLVANIFPFCRNSYILILKKNKT"
     gene            complement(11012..11701)
                     /locus_tag="PKZ29_00470"
     CDS             complement(11012..11701)
                     /locus_tag="PKZ29_00470"
                     /inference="COORDINATES: protein motif:HMM:NF018696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HOG37377.1"
                     /translation="MFNFPNTNKPDFPKAKSLDYGSYWEKRGLVLNKKLKEREEIILK
                     LIEKGKKVLDIGCGNSVLPLMAKEKGIKIEVADVAENVLALHAQHGINGQKIDLEKIS
                     ETVLEDKYDFIILSEVLEHLKNPEEIIQKLKENTGYFLISIPNSAFYRYRWHLLIRGR
                     FFKQWANHPSEHLRFWSHIDFLDWLSVMGLEVVEVLPSNGLSFFGLFSWLKNLWPNLF
                     AHQIVYYCKSC"
     gene            complement(11694..12764)
                     /locus_tag="PKZ29_00475"
     CDS             complement(11694..12764)
                     /locus_tag="PKZ29_00475"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOG37378.1"
                     /translation="MRKTICFFGIYNPTYSRNEVLREGLETHGWRILDCLVDPQKYHG
                     LKKYWKLSVLFSRMRKSADFVLVLYPGQSVLWLARLLAPGKKIIFDAFTSPYDSNVFD
                     RQLYPAKSPLGFKDFFFDWLSWRLADINITDTKENLNYWQEKYSRRDHCLVVFVGSKI
                     KAKSTLTRQPRLDNRFIVHFHGHYIPLQGVSYIIEAAKILADCKDIIFRVIGKGQEYE
                     KVQRLTRKLGLSNVEFFPEMNYEKLKEKITEADIVLGIFGHTPKTARVIPNKVFEGLA
                     MGKPVLTADTPAIAELLEDNINVSLCRQADPRSLAQKIVFLEKHEQLRKQIGQNGKHL
                     YNERLRPGKIVEQLSSTLENYV"
     gene            complement(12767..13801)
                     /locus_tag="PKZ29_00480"
     CDS             complement(12767..13801)
                     /locus_tag="PKZ29_00480"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOG37379.1"
                     /translation="MKLLVITQKVDKNDPILGFFHRWLEEFTKHVEQMTVICLEKGEH
                     RLPGVKVLSLGKEEGRSRLKYVWRFYKYLWQERKNYEAVFVHMNPVYIILAGFWWKIS
                     RKSIYLWYTHRQVDLKLRIAEKFVHKIFTAAEESLGLLTPKKIVMGHGIDLEKFNCFG
                     QKKQSNRLVILHVGRLTKIKNCIVLLNTAELLKKKGVSFELKFVGGPVTLEDQDYLKF
                     LQEFVKQAKLESFVQFVGFVSNDKMPHYYYQADLTVNLAPTGGIDKAVLESMACGVPV
                     VVFNKAFVDYLDQWSSDLLLRDLSPIELSEKIISLVDKKEEIGIDIKEKIKGKIDLSV
                     FVKKLLKEIA"
     gene            complement(13933..14445)
                     /locus_tag="PKZ29_00485"
     CDS             complement(13933..14445)
                     /locus_tag="PKZ29_00485"
                     /inference="COORDINATES: protein motif:HMM:NF039500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5680 domain-containing protein"
                     /protein_id="HOG37380.1"
                     /translation="MENVPNKKIEEKLLNIKKFLVEANKSGYAAGETKNWTKEVDCSQ
                     TIVYESPDGNWLYHDNFFGGEPYGGREVVSWEGQPVWMMVYYGEVIDKTIDKKTFYNF
                     LQKALMGDNLELPVRGPAQLREEINGQEWVYQMTSTGDMARFKGKETIAVDKREIYNA
                     DFSGGLVDVH"
     gene            complement(14495..15607)
                     /locus_tag="PKZ29_00490"
     CDS             complement(14495..15607)
                     /locus_tag="PKZ29_00490"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOG37381.1"
                     /translation="MISTDRKIFEETSAVRARMAEYASLFEKLEIVVFAQKSEGFSIV
                     QIAPNCRIYSTNSLTPLFYIWDALKQGRKILKGEIPAGWVITCQDPFETGLVGWWLTR
                     EFCIKLNLQVHTDVFNPFFITESIKNRIRAWLAKKLLPKADSIRVVSERIKKSITNLN
                     LKTEPIVLPIFTSSRPNLLATKDDLHKKYPQFDFIILMASRLTQEKNFSLALRSLRQV
                     TKKKPKLGLVIVGNGPERKKIEAEIMHLGLQNNVTLEKWNDNLSDYYQTADLFLLTSN
                     YEGYGRTLIEAAEVGCPILTTDVGIVGEILNDDNSLICHVGDEDCLAKKIIWAAANQS
                     ELKKCAQELLSELPQKVIFNQGEYLKKYRDSMEDVE"
     gene            complement(15663..16781)
                     /locus_tag="PKZ29_00495"
     CDS             complement(15663..16781)
                     /locus_tag="PKZ29_00495"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOG37382.1"
                     /translation="MSCKYDLLIATGLFPPDIGGPATYSKILSVELPKRGILVKVLSF
                     GEVRHLPKLVRHLVYTWKVFRLAAKSEVIYAQDPVSVGLPVMVACALRCRPYFLKIVG
                     DYAWEQGSQRFRVIDLLDVFSIRTNYAWPVRVLKKIQLSVACRAKKVIVPSEYLKKIV
                     SNWGTPLEKIQVIYNAFEAPGILPGKVSLRQKLDFNGPVITSVGRLVPWKGFPLLIDL
                     IPELTVQFSGLKLFIIGEGPEREKLEKQIIDKKLTNVFLIGRLPQSELLKYVKASDVF
                     ALNTSYEGFSHQLLEVMWCETPIVTTAVGGNVELIENGKTGFLVGHGNHNDLKEAIEK
                     ILSDHDLADRLAEAAKTKVSEFGKDRMIEKLLNILSLN"
BASE COUNT         5116 a         3973 c         2995 g         4885 t
ORIGIN      
        1 tagcatcttt aaaggaccgc ctgaaggcgg gggttttgct cccaaagatg gataacaata
       61 aaagcgcctt cctcagaaag cgctttggat aaagaacaaa acatcagaac atcagaagag
      121 gttgtccacc cgcctaagct tttccattat cttggcttct ggcgacaaaa cccgcttgat
      181 cccatcgcct cgaagcgtct ccagacgacg gatttgagaa accatattct tgaactccaa
      241 cggttcaagc gaagctttgt ggtcagagcc cgacatattg cggtcaagtg tcagatgggc
      301 ctcaaccagc tccgctccca tcgccaccgc cgcaaccacg ggaaaagtat cgcgggcgtg
      361 gcttgaaaat ccaaccggta caccggggaa cgctcggcgg agcgtctcaa gcaccagtag
      421 attggtgtcc tcgtccgctg gcggatatac cgcaagacaa tgcatcaggg ctaagttctc
      481 gcgacccaag attcttaccg cttcctgaat ctgcctaatc gttgagccgc cagtagacaa
      541 aaccaccgtc ttacccgttt cgcgaactcg ctcgagcaag tctcgattgg taagacaagc
      601 cgaagcaatc ttaacccaac cgattgaaaa accgttgatg aagtgagccg agagtccgtc
      661 ccacggagag gcagaccaag caatgccgag ctcggcacaa cgcttatcaa tcaggtcgta
      721 ctctttcaac ccaaattcaa gagcatgttt gagcactcca tttgtctgcc actcccccgc
      781 cataattcgc gccaacacat cgggcggaaa gacgtcataa cgcacaccct caatctcttg
      841 acgttcaagt gccgcgtcaa gaatttcgcg gtcaaagtca cgcggtgttg ccaactcctc
      901 aggcgagtaa acaatattca ccgccctttt ctgaaatttg accgcatcaa ccccaatgtc
      961 tgccgccacc tcgattgact caagagctag aggcaaactg ccattgtggt taatgccaat
     1021 ttcggccaca acgaaagtcg gacaaccgag cccgacaggt tttccaccga ttttcatatg
     1081 acctctcctt cttcattctt cgggttgctg tttttcttca ggttactgtc caaccgccgt
     1141 ctataacaag agtctgcccc gtcatgtacc gagacgcgtc agaggccaaa aacaacaggg
     1201 gcccgaccaa atcggttttc ggaaccccca accttttcag caaagtccgc cgcttaaggc
     1261 gttccgtaaa ttgctggttc tgattttgtt ttgaattatc agaatcacca taatttggga
     1321 acgcccccgg caccagcgcg ttggccctga tttctggacc aagccaggcg gccatatacc
     1381 gagtcaaggc gaccatcgcg gccttggcca ttccataact ggcaggattg aaatagtccg
     1441 tgtcttcata gaggctcggg tcgggcgcga ccaggccata catagaggca atgttgataa
     1501 tgttgccatt acctctggcg cgcattccag taacaaacag ctgcgccagc ttgaccgccc
     1561 accagtcagt ttttagagac cctacaaaac cttctgagga catcgtctcc agtcgtgccg
     1621 attggtctcc aaaaccagcc gctttccagt cataggcgtt gttgacaatt atgtctacac
     1681 cgccttggct ggtaatggag ttacacaagt tggccaattc aagctcttga tccaagtcaa
     1741 tctgacaaag cttgaggtta gatgaagtga ccgagaacca cgacaaatcg cggtcaaggg
     1801 cataaaccat tgccgctcct gccgaacaca acgactccac aaaaacctgg ccaaaaaacc
     1861 cgcctgcccc cgtgatcaat acatgcattc cttccaaaga aaacatggaa acctccttat
     1921 actgccttta ggcagtttct gctaatgaca aagaaatcca aattaatttc taccatttag
     1981 actaaaaaaa atcaactcct tcccaaatca aggaaactaa ttttacttac aaggcaagct
     2041 gtaaggatca atttttttaa ctgaaacaag taagttggct agatcgcgga ccgcgccgaa
     2101 accgcccggc ctgtcggtaa cccaatcggc caattctttt atcgtatttt cggcactaac
     2161 aggtgccact ttaaaacctc cgatttttaa cattggcaca tctaccaagt cgtcgcccat
     2221 caccgcgcac tcttccactt ttatcttttt ttctttaagc caggcagtta agatgtctcc
     2281 tttgctctga gtatcttttc ctccaaaaag ttcaaccatc ggccaatgat tgtcgccttt
     2341 atttatctgg ctagaaggca aacgattcca gttatcgact aaccttttta ttggttcaaa
     2401 cccaggtgtc cccttttttt ccgcggtaat aaaaacaact gaaagaccga tggccctcat
     2461 taacgatacc ccttgtccat cgacccgact acgaaatttg gctacctgtt cggccggcaa
     2521 taaacactca acatcaggca acaaacaacc atccacatcc atcgcgacag ccttaacccg
     2581 agaagccacc tctttcaatt tcttcccttt aattaacata tagtgtcagt ttaacataaa
     2641 ttttttacca aagatataaa cttgcgaaaa caaaaaaact aaggtaaatt gaccttggaa
     2701 ttgagaacct tggaaataaa acaactatag gggttgataa tatgggaacc ttctcgatcc
     2761 ttaagggctt acaagtggcc ctcatcaaca tgccgatacg tgaaggggca aagcccaaca
     2821 ctccgccttt agggccgggg ttgttggccg cccagttgcg tcagtttggc gcttctgtta
     2881 gcctgattga tctaaatgtc tatcggctta aagacaagct ggcagaacgt cagtccctcc
     2941 tcaacggtcg tcacctctct cttgaagaag caaccggact aatcagccgg tacttcaatc
     3001 gcgatggcga accatgcctg atcggtttgt ccgggatgat caccaccctt aagtggcagt
     3061 cgcagattgc ttccgtttgc cgtaagatgg tccccagggc atgcctcgtt tccggcggtg
     3121 gtcttgccac cgaaatgaaa ggagtgttgt ttaattggat acccgagctc gacgccatcg
     3181 tcttgggtga aggagacaaa gccgtgatcc ggttggctct ccaagtgaaa aaaggcaaaa
     3241 tcaaaccggg agaaaggatc tcccttaccg gcaccagacc cgatctggat gccttgtctc
     3301 tccctgcttg ggacctaatg gaacaagatg ttgacggttg cccgatcctg gaacattaca
     3361 tccgaactcc ggtttggggt ggttcgggca ctcaaaacag ttcggccgcg cctttcgcca
     3421 tggaaagaag tttgacgact gtttcctcac gtggctgccc ctacaattgc gcgttctgct
     3481 ttagaggggc tcagggagcg cgaaactatg ggcagcgcag cgcggacagt ctgatcaaag
     3541 aagcctgctg gcttcgagat cgttacgcca ttgacttcct tggttttaat gacgacaact
     3601 ttgccgtcat gccggaccgc ttgaacctgc tggcaacaag gttccccgac gaagtcggac
     3661 ttcattgggg aactcatatc aggctggaca tggcggacga ccggttgtac accatggcca
     3721 aggccggctg tatttacgcc ggttatggcg cggaaagcgc cagtccaagg gtcttaaccc
     3781 taatgaataa gggcggaatg atgcttaccc aaggacttgt gcgcgtaaac ggcttcttat
     3841 ttccgcggac aatggttgag ggtgtcatca agacgcaaga ggctggcata cacggcaatt
     3901 gcacctggat catggggtat ccgggagaaa agctggacga tctaaaaacc agcgtcggtt
     3961 tcatactctg gcaagttgaa aaagccaccg aaagtttagc ttccggtacc cgagaatacc
     4021 agggggcatc tgaggccgtc aaccaaaata tgttcatggc aacagcctat ccaggcacgg
     4081 caatgttccg aacaaagcca gttcgcgaga gattgtctcg acaatttggc ttaaagtttt
     4141 ccaccaaggg ccgagttatt gccgattcag ccttgcgact ttacgttgag tcactgggag
     4201 atgccgctaa cgtgataagc gataaaaacg gcaaccccat aaacttcagc gatatctctg
     4261 acgacaagct tctgattgcc cgaagcttaa tctcacaagg gaaaatcggc aagattctca
     4321 atctatgacc tttttaaggg ggttcgctta acgccaaccc ccttattttt tataagcaat
     4381 cattagggaa atttctttag ggaaattttt tacgggcggg caaaatagca aatctgatag
     4441 ttccaaatca ttgtaatttt tatttcggta ccatcgattt gtaaaaatta tttgccattt
     4501 gtttggcaaa agagtaagac aaatttttag aagatgcgct aaccaacctc cggcaattcg
     4561 cgccaaatga aaatgttttg ttgatgagaa accggccttg gaaaaagcct gctgccaatc
     4621 caaccaacga tattctcttt ctccattttg tcttctggta tggctgattc gggaaggcaa
     4681 acccatcaat atttttgttt gcggatcata ttccttatct aacaattttt ccaaatcttc
     4741 tttggacatt ctgtcggagc gcgccttgtc aaaacaaaaa ataaagcccc ctggttttaa
     4801 aactctaaaa acttcggata aagttttaac caagtcaggc gagtgatgca gagaaaaaaa
     4861 gtcaaaaaca aaatcagctt gattatcggc taaacctaaa tagtcaaagt cacccacgca
     4921 agacgtaact ttttctgttt tattccccaa gacaaatttt attaccttgg gtgacaaatc
     4981 gcggacaata ttctcaacaa tctccaccgc ataaactttt tctacggaag gcaaagaagc
     5041 aattactgag ctcataaatc ccacccccga accaagctcc acaccaaccc ctttcaaacc
     5101 atgatttaaa attttgcgat caacccaccc aaaaagttca cgcacttccc ccaactcaag
     5161 gtttggagca actgaatcaa aggtcaagcg gctgataatt tcccaaggat ttttatccct
     5221 taatttcaga gctaatttag tccgatagtg attgtctgtc tctcttttaa cgttgtcatt
     5281 tatgtgccat tttatataat ccaaggctgg tttgcgatta gacataatat tgtgtattat
     5341 agcctagtat gaaaatttta ggaataattc cagcccgcgg aggctcaaag cgagtgccca
     5401 acaaaaatat aaaaatggtg ggcggaaaac cactgattgc ttggtcaata gaaagcgccc
     5461 taaaaagcga cctggataga cttgtcgtct caactgatga tgaaaaaata gcaagaattg
     5521 ctaaaaaata cggtgccgaa gttcccttcc taagaccagg agaattatcc ggcgacaaac
     5581 taggcatcga acctgtgtta aaacatgctt tagaatggct acttgaaaaa gaaaatttta
     5641 aacctgaagc agtggccctc ctaatggcaa ccgctcctat gcgcagaccc gaacatataa
     5701 atcaagccat aaagatattt aaaaacaaaa aagcggattc ggttatttcg gtgatagaag
     5761 caaaggccaa ctccaatcca cattggctac taaaaaaaga ttctaaggac caactggttt
     5821 tgtttacagg taaacctttg acaaaaataa taactcgaag ccaagattta cctgtttgtt
     5881 attccagaaa tgatataatt tatgtgctca aacccaaaaa tttatacgat aagaccccca
     5941 atttatatgg caaaaaaaca gaactccttg taatggacga tttttataat gtcgatataa
     6001 atacccctga ggatcttttt atctgcagac aaaaaataaa actcttgaaa aagcagaaca
     6061 ttctctagct tgccctatct tgttttcttt tttataaaat taacaattct ttttccactt
     6121 tgcccatcca gcttaaaaag ctgctgccta agcattttgt tacgacccat ctggtcaagg
     6181 tttgggtcag ataaatattc tctcaccgtt cccactaatt cactctcgga cgaaaccacc
     6241 ctgactcctc cacaagcgat catttttttc atgtgctcct cgtcatgata acggctgaca
     6301 ctatgccaat aatctttttt ttcataacca tcaaaatcaa ttataatctg tggcttgtta
     6361 aagactagcg cgtcaaggcc aagggtggtc gctgtataaa taagaagtga tgaatgatac
     6421 aaagtatcag ccaaatgttt gctatctaaa ctcgttaact ccgtttcctt tggattattt
     6481 tgattaaaaa ccttgcctgg attttctatc acaaaatcta aatcattttc aaaattaaaa
     6541 aaatccgctg gatgattggg gtggtttctg attaagaatt gaattttcgg caatcgccca
     6601 tcaatttttg ccctttttag catatcggca atttgccaat ctgtatcaga taaaattttt
     6661 ccagccggag cgataacaac aaaaggcctg ttggcatcaa ggccgacttt ttgacaaaac
     6721 tcttgcctag ttgaacgtgg ctcgtttaca aaacggtcaa attgaggcaa cccggcaata
     6781 aaaacatcct gaggattgac atcgtgcata caaactactt cttcttttag agtttcgtta
     6841 ttaacaatca accaatcagg aaccacccgc attataccct tgctatagca attatcccaa
     6901 gaacgtatca tccccacagt tgggataccc ctcttttttg cttctcgcgc tatcaacaca
     6961 tcactttcat caaatatgtc agtagaaaaa actattgctg gcgaagaaga ctgaaataaa
     7021 cctataattt ctttttgact ttgacccaac ataaaaaaat atctcatcaa aaaacggact
     7081 ggtcgtttcc ccgccaaaag ccctgtcgcc aaaacttcaa agcaataacg aatacttttt
     7141 ataattttat tgcccttggc attttttctt tcaattttct tataattaag ataatgcgaa
     7201 aattgcaaca aataagccaa gcgagaaaaa aatttggttt ttttagaatt aagcaattta
     7261 gttgaattaa tcccaatgac acaaacattg tctccgccgt aagtcatttc ataaaaattt
     7321 tttttggggt tgggtactac caataaaata ttaaggtctg attgtctcaa atctttaaaa
     7381 acatccgtgt ccaaaatgtt tttggatata aaactgtgaa aaaccgtgat aagaatagtt
     7441 tttttactgt tcatttttaa tataaattat tattccttgt ccaaacaagg agggcaagag
     7501 attattaagg ccaggaagcc cctcataaat aattttcttc tctaatttta acccaagaaa
     7561 accaacccaa tctactatgt ctttagcggt ccaaaaacgt aaatgctcgc ccgggtggac
     7621 tatccattgg agtggaaaac gccccaataa caatcgtacc cgatgttggt aataaccgga
     7681 attaggcacg gaaaatatca gcccctttct aactttattt ttaacaaata gaataaattt
     7741 ctcagggtta gccaaatgtt ccaaaatttc aaaaccggtc aaataatcgc aagggggcac
     7801 atcattccaa tttttttcat catttaaatc taaaaacaaa gtgtccaatc cccttctttt
     7861 ggcctgctcc aaggctagtt cattatagtc agcgcaaatc ccttttattt tttttagttt
     7921 cacccaataa gcgagcattt ccccgctgcc accaccaaca tcaacaacaa cgttgccatt
     7981 ttccaataac ggcaaaacca aatcggctct ttgcttttgc caagacgata acacatccaa
     8041 aaacccactt ggccttcttt ttttccaata cttgtcataa tcaacagtgc cgtccttccc
     8101 aaaatcgggc ggtggataac caataatctt tttaagatcg ttttttactt tatgcaaaaa
     8161 cagtttaaat ttcatctttt gttttgtaca aatttaaata tcgcttggca ataaacgacc
     8221 agtcatgatt ttgagcccat tttgtggctt cttcggacat catactatac aatttttggt
     8281 cagacaaaat cttgattatt aatctagcga tgtctttagg atcattgggg cgagataaaa
     8341 agccagtttt tccatccttg atggcttcct ctcccccatt ttccagagag ccgaccaccg
     8401 gcaagcctct caagttagcc tctaaataga ccaggccaaa tccttcaaaa ttatactggt
     8461 cagaaattgg cgtcaaaacc aataaacaag cttgggtgta aagaaaatcc aattcttgat
     8521 cgcttatatt ataaaaaaac ttaaccctct ccccaatttg attttcattt attatatcca
     8581 ggagcttaca gtaataatct tgatcatcgc gactcccgac aatccagtat tcaaggttgg
     8641 gaaaaatttt tagaatatcc ggcaaggcgc gcagtaccaa atgaaaccct ttcctttttt
     8701 taagcgctcc aactcccagc aaaacctgac ggccgttgcc acaacctgcg cgaggggacg
     8761 atagttgcgt gtttgttgtg tttacaccat ttgggattac ttctatattg cccaagttgc
     8821 cgactgccgt tatcaatcta tctttggtgt aagaactcac gcaaattact ttatcggcca
     8881 attgatataa tttcttatga atagagccga ccaccctaaa agccaatggt tttaaaccga
     8941 acgagccatg aaccgttaaa aaaagtggac agctatataa tttggaaatc aaccaagcgg
     9001 aaggtagata ggtttctacc aaacaatgga caactgtttc ttttccgtct ttaatccaga
     9061 aaacaatttt tatgaccgag agaaaaaaaa gaaaatagtt ccgatagtaa cttagcggac
     9121 ctggcaacaa ttctatctga gctatcgcct cattattgtt agctttgtta caaacaacaa
     9181 taacttcatg gttaaatttt ttataaacac aagccaattc caacgaataa cgtccccagc
     9241 catttttttc ggacaattcg cttgtaaaca aaattattct cataaggtcg ccaaaaattc
     9301 cttaattttt tgagctcggt ttagccaggt gtgttcccga acgtcttttt ttgcttgtat
     9361 ggctaaatta tttgcctgtt cagaattaat cagaaccgac ttaaccccat tttttaaagc
     9421 ttctgccaag tttggagaaa ccaagaccgc gttattttca tttaaaactt ctctaattga
     9481 aggcaggtca gaggcgacta ttggccggcc agaagccatg tattcaaata attttaaagg
     9541 tgaagtacat tcctccgata aacgatctcc ggttttattt ggcaaaagca aaacatctgc
     9601 cgccttttgg tagtaaggca tatctgaatg aggacgaaaa ccaaccaatt ggatattctt
     9661 caagcaattc tcttttttta tttttttcag atctttgtca ttgccgccca ccaaaacaaa
     9721 caatacgtcg tcaccaaata atttcgccgt ttctaaaata acatcaaccc ctttccagcc
     9781 ataaagataa aaactgccgg tatataaaat tattttttta tcaatcggca atccaagtct
     9841 ttgtctaatc actttttctt tttcaaagat atcaaattca gccaaatcaa ccccatctgg
     9901 tgaaaccaga catttttttg ccggataggt tagcccaagt tcgcgtttaa taaaactggt
     9961 cagaacaata aaagacttag cccgaccaaa caaccatcga tgataaaatc tcatttttac
    10021 gggaagcata tggacctcca aaaaaaagcc tttcaaaaac agcgccagta acggttctct
    10081 aatataaatc tgactttttt tgttttttaa aataaaaaac ctcaaggata ggccaaaagt
    10141 caaaatcatc aaccaaaaac ggagacggtg ccacggaata aaagtccaat cagcacaagg
    10201 caaataaatt acctccacct tatttcttaa cttataataa tcgctaaagt cttttttatc
    10261 aggattatga cgtctaggag ccaccaaaat taatttcacc cctgattcct ggaaagcctc
    10321 actcatttta gctatttgat aaccatgggc cttggttgtt ggtaaacgcg cgcttgttat
    10381 gtataaaagt ctcatgtttt gttttttttc aagattaaaa tataagaatt ccgacaaaac
    10441 ggaaaaatgt ttgctaccaa ggaggtcaag aaaaacaaaa ttttattgtt ggcccaatgc
    10501 cctaaataaa ccaccttgtc catgaccagg gtatcaatgg cgtcaaccct ctccaaacta
    10561 aaaggcctaa ggattttact tattgccagc ggcggataag gctttatgtg accaaaagta
    10621 ttccaaattg acatcggcat cggcgagatc aagataattg ttccatgttt tttcaaaacc
    10681 cgattggctt ccttaagcat cgcgtaagct tgatctggaa acaagtgttc aatcaagttg
    10741 ctgcaataaa ccgtctcaaa ttgattatcg gggtaaggca gatttgaagc ctcggccaat
    10801 cgtgcttgat agccttgact ttgcaaatca ttcacgacag tcgggtttgc gtcaatacca
    10861 aaaaaattct gcagatcttt ttttaatatt tcacctcgcc cacagccaac atccaaaacg
    10921 cgcctatttt ttaaataatt atcaaaaatc aggtcacgac caaaaacctt gcccacgaga
    10981 tatcttagtc taaaaattaa gtttttcatt tttaacaact tttacaataa tatactattt
    11041 ggtgcgcaaa taaatttggc caaagatttt ttaaccaaga aaacaaacca aaaaatgaaa
    11101 gaccattgga gggcagaacc tcaacaactt ccagccccat aaccgaaagc cagtccaaaa
    11161 aatcaatatg tgaccaaaac cgcagatgct ccgaaggatg atttgcccac tgtttaaaaa
    11221 accgccctcg tatcaataaa tgccaacgat aacgataaaa cgcgctatta gggatagaaa
    11281 tcagaaaata accagtattc tccttcaatt tttgaatgat ttcttcggga ttttttaggt
    11341 gctcaagaac ttcactcaaa ataataaaat catacttgtc ttctaggacc gtttcgctta
    11401 ttttttccaa atcaattttt tggccattaa taccatgttg ggcatgaagc gctagaacat
    11461 tttctgccac gtcagcgact tctattttta ttcccttttc ctttgccatt agaggcaaaa
    11521 cggaattgcc acaacctatg tctaagactt ttttgccttt ttctatcaac tttaagatta
    11581 tttcctctcg ttctttcagt tttttattca aaaccaggcc cctcttttcc cagtaagaac
    11641 catagtcaag acttttagcc tttgggaaat caggtttgtt tgtgtttggg aaattaaaca
    11701 taattttcca aagtactgga taattgttca actattttcc ccggcctcaa cctttcatta
    11761 tatagatgtt taccgttttg accaatttgt tttctaagct gttcatgttt ttctaaaaaa
    11821 acaatttttt gtgcaagtga cctcgggtca gcttgccgac acaaagaaac attgatatta
    11881 tcctccaata attcagcaat ggctggtgtg tcggcggtta aaaccggctt acccatagcc
    11941 aatccctcaa aaaccttgtt ggggataacc ctagcggttt tgggtgtgtg tccaaaaatt
    12001 cctaaaacaa tatccgcttc ggtaattttt tcttttaact tttcataatt catttctgga
    12061 aaaaactcca cattagaaag tcccaatttt cgcgtcaatc gttgaacctt ttcatattct
    12121 tgccctttgc caataacccg gaaaattatg tccttgcaat ctgccaagat tttggccgct
    12181 tctataatgt aactcacccc ctgcaaagga atatagtggc catgaaagtg gactatgaaa
    12241 cggttatcca aacgcggttg ccttgttaaa gttgacttag ctttaatctt ggaaccaaca
    12301 aaaacaacca aacaatggtc gcgccgacta tatttttctt gccaataatt taggttttct
    12361 tttgtatcag taatattaat gtcagccaaa cgccaactca accaatcaaa aaagaaatct
    12421 ttaaatccta agggactttt ggccggataa agttgacgat caaagacatt ggaatcataa
    12481 ggtgaagtaa aggcatcaaa tataattttt tttccagggg ctaacagtct ggccagccag
    12541 agaacgcttt ggcctggata taaaacaagc acaaaatccg cgcttttcct catccttgag
    12601 aatagaaccg agagtttcca gtattttttt agaccgtggt atttttgagg atcaaccaaa
    12661 cagtccaata ttcgccaacc atgagtttcc aaaccctcac gcaaaacttc gtttcttgaa
    12721 taggtcggat tataaatacc aaaaaagcag atggtctttc tcataattat gcaatttctt
    12781 ttaataactt tttaacaaaa actgataaat ctattttgcc tttaattttt tccttaatgt
    12841 caattcctat ctcctccttt ttatcaacca aggaaataat tttttcgctt aattcaatcg
    12901 ggcttaaatc tctgaggaga aggtcactag accattgatc gaggtaatca acgaaggctt
    12961 tattaaaaac aaccacgggc accccacaag ccatggattc taaaacagcc ttatctatgc
    13021 caccagtggg tgccaaatta acagttaaat cagcttggta gtaataatgg ggcattttgt
    13081 cattagagac gaagccaaca aactggacaa aactttctaa ctttgcctgt ttgacgaact
    13141 cttgaagaaa ttttaaatag tcttgatctt ctaatgtaac aggcccgcca acaaatttta
    13201 actcaaagct gactcctttt ttcttcagta gctcagctgt gtttaagaga acaatgcaat
    13261 ttttaatttt tgttaatctg ccaacatgta aaatgaccaa ccgatttgac tgtttttttt
    13321 gcccaaaaca attaaacttt tccaaatcaa taccatgccc cataacaatt ttcttagggg
    13381 tcagcaaacc taggctttct tcggcggctg taaatatttt gtgaacaaac ttttccgcaa
    13441 ttcttaattt caaatctact tgccgatgag tataccaaag ataaattgac tttcgcgaaa
    13501 ttttccacca aaatcccgcc aatattatat agactggatt catgtgaaca aaaactgcct
    13561 cgtaattttt tcgttcttgc caaaggtatt tataaaaacg ccaaacgtat ttaagacggg
    13621 accgcccctc ttctttgcct aatgacaaaa ccttgactcc gggcaagcgg tgctcgcctt
    13681 tctccaaaca gatgactgtc atttgctcga catgtttggt aaattcttcc aaccaacgat
    13741 ggaaaaagcc caaaattggg tcgtttttgt ctactttttg cgtaattacc aaaagcttca
    13801 caaaattatt ctagcacaaa aacagcccaa aaacattaaa aggctaagct cgccacggca
    13861 ggcggagccg gttaaaatgc ttctaaataa ggtcagtttt attttaaatc aaacaagctt
    13921 ctcgcccctt ttttaatgta cgtccaccaa cccacccgaa aaatcggcat tataaatttc
    13981 tcgcttatca acagctatgg tttctttgcc tttaaaacgc gccatgtcac ccgtacttgt
    14041 catttgataa acccattctt ggccgttaat ttcttcgcgc aattgcgccg ggcctcgcac
    14101 cggcaattca aggttatcac ccatcaaagc tttttgtaaa aaattataga acgtcttttt
    14161 atctatagtt ttatcaatta cctccccata ataaaccatc atccaaactg gctgaccctc
    14221 ccaagacacg acctcgcgac cgccataagg ttcaccgcca aaaaaattgt cgtgatataa
    14281 ccaattgcca tccggacttt cataaactat cgtctgtgaa caatcgacct ccttagtcca
    14341 attttttgtt tcaccagcgg cataacccga tttgttagct tctaccaaaa actttttaat
    14401 gtttaacaat ttttcttcta ttttcttatt gggcacattt tccatatttt aagttaaatt
    14461 taaaatttta tctttattct atattcgaca tcacctattc aacatcctcc atgctatctc
    14521 tgtatttttt caaatactct ccctggttaa aaataacttt ttgaggtaat tctgacaata
    14581 actcctgcgc acactttttt aattcactct gattggctgc cgcccaaata atttttttgg
    14641 ccaaacaatc ctcgtcacca acgtggcaga ttaagctgtt gtcgtcattc aaaatttcgc
    14701 cgacaattcc cacatcagtg gtcaaaattg gacagccaac ttcggcggct tctatcaatg
    14761 tccgcccata gccttcataa tttgaggtta gcaaaaacaa atccgcagtt tgataataat
    14821 cggataggtt gtcattccat ttttctaaag taacattgtt ttgtaaacca aggtgcatta
    14881 tttctgcttc aatctttttt cgctccggtc cattgccgac aatcaccaaa ccaagtttcg
    14941 gctttttttt agttacctgt ctcaagctcc gcaaggccaa ggaaaagttt ttttcctgtg
    15001 ttaaacgaga ggccattaaa attataaaat caaactgcgg atattttttg tgtaaatcgt
    15061 ctttggttgc caacaagttt gggcgcgatg aagtaaaaat tggcaaaaca attggttctg
    15121 ttttcaaatt taagttggta attgattttt ttattcgctc cgataccacg cggatactgt
    15181 ccgctttggg caaaagtttt ttcgccagcc atgcccgaat cctgtttttg attgattcgg
    15241 taataaaaaa aggattaaaa acatcagtat gtacttgaag attaagcttg atgcaaaatt
    15301 ctctcgtcaa ccaccagcca accaagccgg tttcaaatgg gtcttggcaa gtaataaccc
    15361 aaccagcagg gatttcccct tttaaaattt ttcgcccttg ttttaaggcg tcccaaatat
    15421 aaaaaagcgg cgttagactg tttgtcgaat agattcgaca atttggcgca atctgaacta
    15481 tactaaatcc ttcacttttt tgggcaaaaa ctacaatttc caatttttca aataaactgg
    15541 catactcagc catccgcgcg cgcaccgcgc tcgtctcttc aaatatttta cggtcagtag
    15601 aaatcatcag aacatacata atttaaaatt taaaagttaa agtaatcaag taaaaagttg
    15661 aatcaattta aagacaaaat atttaacaat ttttctatca ttcgatcttt accgaactca
    15721 gataccttgg ttttagcggc ttcggccaga cgatcggcca aatcgtggtc agacaagatt
    15781 ttttcaatcg cttcttttag atcattgtga ttaccatgac caaccaagaa accggtttta
    15841 ccattctcta tcaattccac gttgcccccc actgccgtcg ttacgatggg cgtctcacac
    15901 cacatcactt caagcaactg atgagagaac ccttcataag aggtattgag cgcaaaaaca
    15961 tcggacgctt tgacatattt taaaagttcg ctttgtggca gacgaccaat taaaaagaca
    16021 ttggttaatt ttttatcaat aatttgtttt tctaattttt ctcgttccgg tccttcccca
    16081 attataaaaa gtttcaagcc cgaaaactgc acagtgagtt ctggtattaa atcaattaac
    16141 aatggaaacc ccttccatgg caccaagcgg ccaacgctag taattaccgg cccgttaaaa
    16201 tccagtttct gtcttagact aacttttcct ggcaagattc ccggtgcttc aaaggcatta
    16261 tagataacct gtattttttc taacggagtt ccccagttac taacaatctt ttttaaatac
    16321 tcgcttggga caatcacttt tttggcccga cacgcaaccg ataattgaat tttttttaga
    16381 acccgcactg gccaagcata gttagttctt attgaaaaaa catccagcaa gtcaataacc
    16441 ctaaagcgtt ggctcccctg ctcccaagcg taatcgccaa caatttttaa aaagtatgga
    16501 cgacaacgta aagcgcaggc caccatcact ggcagaccga cgctgaccgg atcttgggca
    16561 taaatgactt cacttttcgc cgccagacga aaaactttcc aagtatagac caaatgacga
    16621 accaattttg gcaagtgacg aacctcgcca aaacttaaaa ccttgaccaa aatcccgcgc
    16681 tttggtaatt caacgctcaa aattttgcta taggtagccg gcccgccgat gtcgggcggg
    16741 aaaagaccgg tagcaatcag aagatcatat ttgcaagaca taaaagtatg atacagagtt
    16801 aaaagttaaa aggcaaaaat ttaaatcttt ttagattaaa ataaaaactt atttcactca
    16861 cttttgactt tataacttgg tttccccaaa atctccccca cctgttctgc aatcttgtcc
    16921 cagttatatt tttcaaccac caactggcga gctttctcaa ccacttggt
//
LOCUS       DAOSHV010000005        11888 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_4538_length_11888_cov_6.050621, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000005 DAOSHV010000000
VERSION     DAOSHV010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 11888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11888
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4538_length_11888_cov_6.050621"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1766
                     /locus_tag="PKZ29_00500"
     CDS             <1..1766
                     /locus_tag="PKZ29_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HOG37383.1"
                     /translation="RVPSILIFIFCLIVGFVILFDTSVAQVVKFFVTVFNLIKKYTVG
                     SAQKIAKKAKEKEKSKIFQSQLPLEPSSDAKKDKPDPNVVGVPPIFKPNSTIITPTAA
                     LSSSGQQIWKYPSIDIFDNTPGAKADRGDIRKNAQTIEQTLDSFGITARVSEVNDSPS
                     VTQYALEVALGTKLAKIMSLSNDLAMALAAPGGMIRVEAPIPGRALVGIEVPNRSLEV
                     VPIRRMLESDAMKTAKNKITVPLGLDVAGNPKVADITKMPHVLIAGQTGSGKSVCVNS
                     WISSILFKASPDEVRMIMVDPKRVELTPYNGIPHLLVPVIVEPDKVLSALKWAVGEME
                     RRYKTFTEVGAKNIEAYNNLSGFQSMPYILIFIDELAEIMLFSPSEVEDNICRIAQMA
                     RAVGIHLILATQRPSVNIITGLIKANIPTRISFAVASVTDSRVILDTPGAEKLLGRGD
                     MLYMPPDLAKPVRVQGCFVSDKEVTNLIDTLKGQKTTEYDDQILNQPVSGGKVAKNAP
                     STGENLADKDPMFDDAVKLVQESGKASSSLMQRRLKLGYARAARVLDQLEQAGIVGPS
                     KGAKAREILIPHNTPSDDNAE"
     gene            1773..2381
                     /locus_tag="PKZ29_00505"
     CDS             1773..2381
                     /locus_tag="PKZ29_00505"
                     /inference="COORDINATES: protein motif:HMM:NF020700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOG37384.1"
                     /translation="MLASSLLKNTRLDKEWELDDISKKIKIPVKYLQALEDEQVGCFP
                     QEPYCSLIIKDYADYLGLQGQDVLSLFRRDFDQKRKSKNIIKTGFTFTPKFTFTVSIL
                     AIIVLFSLYLVSEYFKFHQPPKLKVNWPLEASVSSSVVEISGITDPESTIRINDDLII
                     VDSDGLFQKKITLSSGDNNIVVESTSPNGKTTVEQKNINSSL"
     gene            2407..2679
                     /locus_tag="PKZ29_00510"
     CDS             2407..2679
                     /locus_tag="PKZ29_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37385.1"
                     /translation="MDITQIVTIISLILITGVVVVSGIWLILVLKQLRTTLGKTNQIL
                     EDTQLITSSVAKPVSSFSEFLMGFKNGFGFFNNLFNRKNKTDDSSV"
     gene            2663..2986
                     /locus_tag="PKZ29_00515"
     CDS             2663..2986
                     /locus_tag="PKZ29_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37386.1"
                     /translation="MTHPSNQSNQNCCQHSSGFVFGLICGAVISAVIAVIIYKNNRTE
                     IFEKLDQKIKQFFDRLSPSKPSSKPKTKPQKPAPTEFIASAKTDITPVFVKKTHPKMF
                     VKPKK"
     gene            3004..4830
                     /locus_tag="PKZ29_00520"
     CDS             3004..4830
                     /locus_tag="PKZ29_00520"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006187823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOG37387.1"
                     /translation="MNARQLKEKFVNFFVARDHVEIPSSSLIPENDPTTLFISAGMHP
                     LVPYLLGQPHHLGKRLTSVQKCIRTGDIDDVGDKFHHTFFEMLGNWSLGDYFKQEMIP
                     WSFEFLTQHLGYQVNQIHVTCFEGDTNAPRDTESHDLWLKQGIPESQIHYLPKKDNWW
                     GPAGTTGPCGPDTEMFIDLDVAAGPIDFVAGCKSERIVEVGNDVFMEYNLTASGQYEP
                     LSQKNVDTGYGAERNTAVINGFTDNYLTDIWQPIIKTIEQISGKTYYQSPEITRSIRI
                     IADHLKAAVFIISDGVEPSNKEAGYVLRRLIRRAIRHGKMLGIDTNFISQIAISVLDN
                     QSNYAGIYPQLDQNRDKILSILDFEENKFRKTLNRGLAELQKDELNYSKTNSNATIPV
                     DKKYLGDKSFYYYESFGFPFEMFYEESTLAKAYPKDEVYQYFQQAQQDHIDQSRNLSA
                     GKFKSGLADHSDVIVKYHTAAHLMHASLRRVLGDHVHQAGSNITADRIRFDFTHPDKV
                     TPQQLQQVEALVNDQIRAALPVTHQTIPFADAKNQGALFLAGHTYPDMVTVYIVGSDA
                     DFYSKEICTGPHVDNTSQLSQFKILKEESAGSGKRRLYATLK"
     gene            4864..7059
                     /locus_tag="PKZ29_00525"
     CDS             4864..7059
                     /locus_tag="PKZ29_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37388.1"
                     /translation="MEGNSFWLIYIQNDRVSVSLISAFDNKYRVLATGPSKSWTSDNP
                     DTLTTAVDESLSVASLNANITEEQEPGSAAIVVPPFWVSNDGKILPSKVKSIKETCKS
                     LSLTPTGFLAEDDAIVEDANQSDGFPASFILVHLSDKDFYLSLVYLGHIKERIKKDLL
                     QEFTSQDLESALLEINSESALPPQILVFGQFDARILSSLKNYPWVGKKNVETFLHLPE
                     IKSYDQNQIIDIFTRVISGQLDSSSFSPKISDTPDDNSKADPPPADTKPEPEPEITAV
                     AQKLTLTETSPEDLGFSSSDSTPAPASTPTLPIITPADIPPQDNPPSFSTPSPPIEPP
                     PSQKPKFSFDFFKKFKLPQIKFNKNFLWLGLIFIPILAVLPFFILKTDVTVFVVPYKF
                     DKSLPVTLKVGADNNDISSSIIPVNKETFDIKAEVSIKTTGQKTVGNKSQGEITIFNK
                     LDKPQSLPKGAVITDPDGKKFELTTAVSVAASSSNLEEGVIKLGQTKTVAVAADIGSE
                     YNIPAGSQLKFKDFPETSLIAKTESAFAGGSRQQIAAVSAEDKTTVQSRINEEITNKI
                     EEKINQTTGSVSGLIKETIQSQKDSLELSREVGEAAEDLAGTITASVSVFVLDNNTRD
                     SVIRQFLSSESDFANSLFDPKDYTFSLKVNKIETNQASGNLTVSGQSLPKIDQSNLKK
                     ALTFKTLKSGGETIKKIIPRAYNFNIKTKLPLLSFTPNHINIEVKTETK"
     gene            7056..7772
                     /locus_tag="PKZ29_00530"
     CDS             7056..7772
                     /locus_tag="PKZ29_00530"
                     /inference="COORDINATES: protein motif:HMM:NF016119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0016485 - protein processing [Evidence
                     IEA];
                     GO_process: GO:0045184 - establishment of protein
                     localization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HOG37389.1"
                     /translation="MSYIYLKKKSKSVPAKIKRRRLLAAGFVFLGAVFFSLAVFPIIK
                     FQFSYSTRLKQIIDPLSAQSYGQPSILGQSATDYTQLSNWFVNSPDLPSLNSQTIDTS
                     VNSYYLTIPKLKINKALVNLGTMDLEKSLVQYPQTALPGHFGNTVVFGHSVLPQFFSP
                     KNYLTIFSTLYKLEQGDEIIVEYDKSTYKYIVEEMFEVKPTNLSVLDQRFDDKILTLI
                     TCSPPGTYLRRLIIRASLSF"
     gene            7775..8146
                     /gene="ruvX"
                     /locus_tag="PKZ29_00535"
     CDS             7775..8146
                     /gene="ruvX"
                     /locus_tag="PKZ29_00535"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOG37390.1"
                     /translation="MNALGIDYGTKRIGLAVSISGIIQPLTTLKNSPEFINQLKQIVS
                     DYQIDQIYVGISQGTMALQTQKFVTRLQNMLTLPISTVEEAVSTIEADRIFLTNKKHQ
                     KDYQKTIDAIAAAVILKRVIN"
     gene            8154..8351
                     /locus_tag="PKZ29_00540"
     CDS             8154..8351
                     /locus_tag="PKZ29_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37391.1"
                     /translation="MIKDLIAPIQAWLLSQKRCVGCGTALSKGTRKPVRGQTTVTCRC
                     GRIFIHDESKKTYRRALFDEI"
     gene            8357..9322
                     /gene="mltG"
                     /locus_tag="PKZ29_00545"
     CDS             8357..9322
                     /gene="mltG"
                     /locus_tag="PKZ29_00545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOG37392.1"
                     /translation="MKKFFLITAGIILVLVLSSVFYVISNLSSVSTDTKTVTFVINKG
                     DGIINVAQKLHSNRLIKDKYSFVAYAVATRQNQKIPSGTFRLSPSLSVKEILVNFASG
                     GISDYWIKIIDGFRVEEIAPLFPEDLSFTPSDFLAKAKSKEGHLFPDSYLLPKYYNLD
                     QVLDFFDQNFTKKISQAKSDSHTTLSDRDNLILASLVEREGRSLAAKKMIAGILLNRL
                     NINMPLQVDASVQYARDSRSKNLAKYWQPVTRDDLKQIDSDYNTYLYPKLPPGPICNP
                     GYDSIYAVYHPTDSDYLYYITGNDNQMHYAKTLDQHNQNIANYLR"
     gene            complement(9319..10374)
                     /locus_tag="PKZ29_00550"
     CDS             complement(9319..10374)
                     /locus_tag="PKZ29_00550"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HOG37393.1"
                     /translation="MKFFIKTFGCIQNTSDSERIKAHYWENGWQEVKNWKEADEVIIN
                     TCVIRQSAENRAYGFLRNVQKLGKKVVVTGCLVGVMNKNNKKKMETKKNFPGIEFRAI
                     DKYVIDTEPIKKFKGVVLVPISYGCNNMCSYCIVPQAKGKEISRSMAEILADVDEAVK
                     KGNKKILLIGQNVNSYGSELSDCGYVFMGKKRFKSLFPNLLAEVAKKDLNQVSFVSNN
                     PWDFSDDLIEVIAKYKNIDRLIHLPFQAGDDEILKKMNRNYTKQEYLDLVKKIKSRVN
                     GARFSTDIIIGFPGESDKAFEKTVDICRQVGFEVAYLNKYSPRPGTVSEKLYPDDVPM
                     KVKKERWVRLNQMINKN"
     gene            complement(10397..10873)
                     /locus_tag="PKZ29_00555"
     CDS             complement(10397..10873)
                     /locus_tag="PKZ29_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37394.1"
                     /translation="MKKILIVLWFLVCVVSFKGEVLASGFNLKSIGSVDTGGRQISHW
                     WYSGTNAVFNGEAPAGSVVTVSVDGSEGTATSDGNNNWSYSAGALSGGDHTVVISNNG
                     STINFTLTMGAENVNWEAVNADAGTTTLPTVGVVLPTVLLSTLGSGLLLAAKKIAK"
     gene            10941..11693
                     /locus_tag="PKZ29_00560"
     CDS             10941..11693
                     /locus_tag="PKZ29_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37395.1"
                     /translation="MDDIKTNPLTDQQDQDNYQEILDKYAASVKPEAEIAPAPENPAP
                     ETVSATLVDTETSIDNQNITPSLEINQPDTNPSVIAPPEPPPEPKTKTPEEIKAEVDK
                     ILADSPDNHPSSETVIAPPTPSSSKFLKTIFIISLIIFLAVVGLLVYFMFFNKPVSTG
                     PVDNNSVPTDVPEVACELNGVEYKLGESFPSADGCNTCVCQSPDVIACTERACNTTST
                     LNTQSTTVAPTATKSGTQNPSPTKSVLPTLGR"
BASE COUNT         3351 a         3014 c         1963 g         3560 t
ORIGIN      
        1 gtcgggttcc cagtatcctt atttttattt tttgtcttat tgttggtttt gtcattttgt
       61 ttgatacttc ggtagctcag gtagtcaaat tctttgttac cgtttttaat ttgatcaaaa
      121 aatatactgt cggcagcgcc cagaaaattg ccaaaaaagc caaagaaaaa gaaaaatcaa
      181 aaatatttca atcacagttg ccattggaac catcctcaga cgccaaaaaa gacaaacccg
      241 accctaacgt tgtcggcgtt ccacccatat ttaaacccaa ttccaccatt attactccta
      301 ctgctgctct cagttcttcc ggtcagcaga tttggaaata tccttccatt gatatttttg
      361 acaatactcc cggtgccaag gccgacaggg gagatatccg caaaaatgcc cagactatag
      421 agcagacact tgattcattt ggtattaccg ctcgggtttc tgaagtcaac gacagtcctt
      481 ccgtcaccca atatgccctt gaagttgctc tgggcactaa attggccaaa attatgagtc
      541 tcagcaacga tttggctatg gccttggctg cccccggcgg tatgattcgg gtcgaagccc
      601 ctattcctgg tcgggcatta gttggtatcg aagttcccaa ccgttctcta gaggtagtac
      661 ccatcagacg catgttggag tcagacgcca tgaaaactgc caaaaacaaa attactgttc
      721 ccttgggtct ggacgttgct ggcaatccca aagttgccga cattaccaaa atgccccacg
      781 tccttatcgc cggtcagact ggttccggta aatcggtttg cgtcaactct tggattagta
      841 gtattttgtt caaagcttct cctgatgagg tcagaatgat tatggtcgat cccaaacggg
      901 tcgagcttac tccctacaac ggtattcctc atcttttggt gccggttatc gttgaaccag
      961 acaaagtcct ttctgctctc aagtgggctg tcggcgaaat ggagcgccgc tacaaaacct
     1021 ttaccgaagt cggcgccaaa aatattgaag cctacaacaa cctttccgga tttcaatcca
     1081 tgccctacat tttgattttt attgatgaat tggccgaaat tatgttattc tctccctccg
     1141 aggttgaaga taatatttgc cgcattgccc aaatggcccg ggctgtgggc atccatttga
     1201 ttttagctac ccaaagaccg tcggttaata ttattaccgg ccttatcaaa gccaatatcc
     1261 ccacccgaat ttcctttgct gtcgcttctg tcaccgactc ccgggttatc cttgatactc
     1321 ccggcgccga aaaacttctt ggcaggggag atatgctcta tatgccgccg gatcttgcca
     1381 agccggttcg ggttcagggt tgttttgttt ccgacaaaga agtcactaat ttaattgata
     1441 ctctcaaagg tcaaaaaacc accgaatacg atgatcaaat tttaaatcag ccggtttctg
     1501 gaggtaaggt agccaaaaat gctccatcca ccggcgaaaa tcttgccgat aaagatccta
     1561 tgtttgatga tgctgttaaa ttagttcagg aatcgggcaa agcatcttca tctctgatgc
     1621 agcgtcggct aaaacttggt tatgccaggg ctgccagggt ccttgaccag ttggaacaag
     1681 ctggtattgt cggcccttcc aaaggcgcca aggccagaga aattctcatt cctcacaaca
     1741 ctcccagcga cgacaacgct gaataatttc ttatgctggc ctcttcctta ctcaaaaata
     1801 ctcgcctcga caaagaatgg gaacttgatg atatctccaa aaaaattaaa atccccgtta
     1861 aatatttaca agctcttgaa gacgaacagg ttgggtgttt tcctcaagaa ccctactgtt
     1921 cccttatcat caaagattat gccgactatc ttggccttca gggccaagac gttttatctc
     1981 ttttccgccg tgactttgat caaaaaagaa aatcaaaaaa tattatcaaa accggtttta
     2041 cttttactcc caaatttact tttactgtct ctattcttgc cattatcgtt ctattttctc
     2101 tttatctggt ctctgaatat tttaaatttc accagccgcc aaaactaaaa gttaattggc
     2161 cgcttgaagc cagcgtctct agctctgtag tagaaattag cggtatcacc gaccccgaat
     2221 ctactattag gatcaacgac gacttgatta ttgttgactc agacggcttg ttccaaaaaa
     2281 agattactct tagctccggc gacaataaca tcgtagtcga atccacttct cccaacggca
     2341 agaccacagt tgaacaaaaa aacataaatt cttccctcta accccaaaat agtctatact
     2401 gactttatgg atatcacaca aatcgtcacc atcatcagtc taattttgat taccggggtt
     2461 gtggttgtct ccggtatttg gctgattttg gttctcaaac aacttcgcac cacccttggc
     2521 aagaccaacc aaattctcga agatactcaa ctgattacca gctctgttgc caagccggtc
     2581 tcttcttttt ctgaatttct catgggtttc aaaaacggct ttggattttt taacaattta
     2641 ttcaaccgaa aaaacaaaac cgatgactca tccgtctaac cagtccaatc aaaactgttg
     2701 tcagcatagc tccggctttg tttttgggct tatttgtgga gccgtcatta gtgccgttat
     2761 tgccgtcatt atctacaaaa acaatagaac tgaaattttt gaaaaattag accaaaaaat
     2821 caaacaattt tttgaccgtc tttcaccttc aaaaccatca tccaaaccaa aaactaaacc
     2881 tcaaaaaccc gctcccaccg aatttattgc ctctgccaaa acagacatta ctccggtttt
     2941 tgtcaaaaaa acccacccca agatgtttgt taaacccaaa aaatagttgg tataattggc
     3001 tttatgaatg ctcgtcagct caaggaaaaa tttgtcaact tttttgttgc ccgggatcat
     3061 gtcgagattc cctcaagcag tttaattccc gaaaacgatc ccaccacctt gtttatttct
     3121 gccggtatgc acccgctggt gccttatttg cttggccagc ctcaccactt aggcaagcgt
     3181 ctcaccagcg tccaaaaatg tatccgcact ggcgatattg atgatgttgg tgacaaattt
     3241 catcacactt tttttgaaat gttgggcaat tggtctctgg gagattattt taaacaggaa
     3301 atgatccctt ggagttttga atttttaacc caacacctcg gctatcaagt aaatcaaatt
     3361 catgtcactt gttttgaagg tgataccaat gctccccgcg ataccgaatc tcatgacttg
     3421 tggctcaaac agggaattcc cgaatctcaa attcattatc tccccaaaaa agataactgg
     3481 tggggtcctg ccggcaccac cggaccgtgt ggtcccgaca ccgaaatgtt catcgacctt
     3541 gatgtcgccg ctggtcctat tgattttgtt gccggttgca agtccgaacg gattgttgaa
     3601 gtcggcaatg acgtttttat ggaatacaat ctaaccgctt ccggtcagta tgaaccccta
     3661 tctcaaaaaa atgttgacac cggttatggc gccgaaagaa atactgccgt tatcaatggt
     3721 tttactgaca attacctgac cgatatctgg cagccgatta tcaaaactat tgaacaaatt
     3781 tctggtaaaa catattatca atctcccgaa attacccgat caatcagaat tattgccgac
     3841 catctcaagg ctgctgtctt tatcatttct gacggagtcg aaccttccaa caaagaagcc
     3901 ggttatgttc tccgccgtct tatccgccgg gctatccgtc acggcaaaat gcttggcatt
     3961 gacaccaatt ttatctccca aattgctatt tctgttcttg ataatcaatc taattatgcc
     4021 ggcatttatc ctcaattaga tcaaaatcgc gacaaaatct tatcaatttt agattttgaa
     4081 gaaaataaat ttcgcaaaac tctcaatcgg ggactggctg aacttcaaaa agacgagctt
     4141 aattattcca aaactaactc caatgctact attccggtcg acaaaaaata tttaggtgac
     4201 aaatcttttt attattacga aagttttggc tttccctttg aaatgtttta tgaagaatcg
     4261 actttggcca aagcttatcc caaagacgaa gtttatcaat attttcaaca agctcagcag
     4321 gatcatattg atcagtcacg aaacctttct gctggcaaat tcaaatccgg tctagctgac
     4381 cattctgacg tcattgtcaa ataccacacc gccgcccacc tgatgcacgc ttcccttcgt
     4441 cgggttttgg gtgatcatgt tcaccaggcc ggatcaaaca ttaccgccga cagaattcgt
     4501 tttgatttta ctcaccccga caaagttaca cctcagcagc ttcagcaggt tgaagctcta
     4561 gtcaacgacc aaatccgagc cgcccttccc gttacccatc aaaccattcc ttttgccgat
     4621 gccaaaaacc agggagccct tttccttgcc ggtcacacct atccggatat ggtcacggtt
     4681 tatattgtcg gcagcgatgc tgatttttat tccaaagaaa tttgcaccgg acctcatgtt
     4741 gacaatacca gtcagttaag ccagtttaaa attctcaaag aagaatcagc cggctccggc
     4801 aaacgccgcc tctacgccac cctcaaataa accaaagagt gagatttata gtatccttat
     4861 aatatggaag gtaacagttt ttggctgata tatattcaaa atgaccgggt ttctgtttcc
     4921 ctgatttctg cttttgacaa caaataccga gttttagcta ctggtcccag caaaagttgg
     4981 acttctgaca atcccgatac tttgactact gccgtcgacg aatctctctc ggttgcttct
     5041 ctcaatgcta atattactga agaacaagaa ccgggttctg ccgctattgt tgtccctccg
     5101 ttttgggtca gcaacgacgg caaaattctc cccagcaagg ttaaatctat caaagagacc
     5161 tgcaaaagct tatctctcac ccccaccggc tttttagccg aagatgacgc cattgtcgaa
     5221 gacgccaatc aatcagatgg ttttcccgcc agttttattt tggttcatct ttctgataaa
     5281 gatttttatc tctctctggt ttaccttggt cacatcaaag aaaggatcaa aaaagatctc
     5341 cttcaagaat ttaccagtca agatttagaa tcagccttgc tggaaatcaa ttccgaatca
     5401 gccctgccac cccaaattct tgtctttggt caatttgatg ctcggattct cagttctctc
     5461 aaaaattatc cctgggtagg caaaaaaaat gtcgaaactt ttttacatct acctgaaatc
     5521 aaatcatacg atcaaaatca aatcattgat atttttaccc gggttatttc cggtcaatta
     5581 gattcatcct ctttctctcc caaaatatct gatactccag acgataattc caaagccgat
     5641 cctccgcccg ccgataccaa accagagcca gaacctgaaa ttaccgctgt tgcccaaaaa
     5701 ctcactctca ccgaaactag tcccgaagat cttggtttta gttcttccga ctctacccct
     5761 gctccagctt ccactcccac tctccctatt atcactcctg ccgatattcc acctcaagac
     5821 aatccgccct ccttttcaac tccatcccca ccaatagaac cacccccttc tcaaaaacca
     5881 aaattttctt ttgatttttt caaaaaattc aagcttcccc aaatcaaatt taacaaaaat
     5941 ttcctctggt taggacttat ttttatcccc atcctggctg ttttaccttt ctttattctt
     6001 aaaactgacg tcactgtttt tgtggttcct tacaaatttg ataaatcgct tcccgtcacc
     6061 ctcaaagtcg gtgccgacaa caacgatatt tcttcttcta ttattcctgt caacaaagaa
     6121 acttttgata ttaaagccga agttagtatc aaaactaccg gtcaaaaaac tgttggtaac
     6181 aaatctcagg gtgaaattac tatttttaat aaactagaca aaccccaaag cctgcccaag
     6241 ggtgccgtta ttaccgaccc tgatggcaaa aaatttgaac ttaccaccgc tgtttccgtc
     6301 gccgccagca gttccaatct cgaagagggt gttattaagt taggtcagac caaaactgta
     6361 gctgtcgctg ccgatattgg ttctgaatat aatattcccg ccggatctca actcaaattc
     6421 aaagactttc ctgaaaccag ccttattgcc aaaacagaat ccgcctttgc cggcggcagc
     6481 cgccagcaaa ttgccgccgt tagcgccgaa gacaaaacca ccgttcaatc ccgtattaac
     6541 gaagaaatta ccaacaagat tgaagaaaaa ataaatcaaa ccaccggcag tgtttccggt
     6601 ctcatcaaag aaaccatcca aagccaaaaa gacagtttgg aactcagccg cgaagtcggt
     6661 gaagctgccg aagatcttgc tggaactatt accgcctcgg ttagtgtgtt tgtcctggac
     6721 aataacacta gggatagtgt tattcgacaa ttcctctcgt cggaatccga ttttgctaac
     6781 agtcttttcg accccaaaga ctatactttt tctctcaaag ttaataaaat tgaaactaac
     6841 caagcctccg gtaatcttac cgtctccggt cagtctttgc ccaaaattga ccagtcaaat
     6901 ctcaaaaaag cccttacttt taaaactctc aaaagcggcg gtgaaactat caaaaaaatt
     6961 atcccccggg cctacaattt taatatcaaa accaaactgc cccttctgtc ttttaccccc
     7021 aaccatatca atattgaagt taaaaccgag accaagtgag ctatatttat ctcaaaaaaa
     7081 aatctaaatc tgtccctgcc aaaatcaaac gccgtcgtct gttggcggcc ggctttgtct
     7141 ttttgggagc tgtttttttt agtttagcgg tttttcccat tattaaattt caattttctt
     7201 attccactcg tctcaaacaa attatcgatc ccttatcagc ccagtcttat ggccagcctt
     7261 ccatcttggg tcagtctgcc accgattaca cccaattatc caattggttt gtcaattctc
     7321 ccgatcttcc cagtctcaat tctcagacca tcgacacttc cgtcaacagc tattatctaa
     7381 ctatccccaa actaaaaatc aacaaagcct tggtaaatct tggcaccatg gatttggaaa
     7441 aaagtttggt tcaatacccc caaaccgccc tgcccggaca ttttggcaat actgttgttt
     7501 ttggtcattc agttttaccc cagtttttta gccccaaaaa ctatttgact attttttcca
     7561 ccctttataa attggaacaa ggtgatgaaa ttattgttga atacgacaaa tctacctaca
     7621 aatatatcgt cgaagaaatg tttgaagtca aacccaccaa cctttctgtt ctggatcaga
     7681 gatttgatga taaaattttg acccttatta cctgcagtcc tcccggcacc tatctccgcc
     7741 gcttaattat tcgtgccagc ttatcttttt aactatgaac gctttgggta ttgattacgg
     7801 caccaaaaga attggcttgg ctgtctctat cagcggtatt atccaaccct taacaacttt
     7861 aaaaaattcc cccgaattta ttaatcagct caagcaaatt gtctccgact accaaattga
     7921 ccaaatttat gttggtattt ctcagggaac aatggctctc caaacccaaa aatttgttac
     7981 ccggctccaa aatatgctaa cattacccat cagcacagtt gaagaagctg tcagcactat
     8041 tgaagctgat cgcatttttc ttaccaacaa aaagcatcaa aaagactatc aaaaaactat
     8101 tgatgccatc gccgctgctg ttattctcaa gcgagttata aattaacaaa actatgatta
     8161 aagatctaat tgctcctatt caagcttggt tattatctca aaaaagatgt gtcggttgtg
     8221 gcaccgccct ctcaaaaggc actcgcaaac ccgtccgggg tcaaacaact gttacctgcc
     8281 gctgcggtcg catttttatc cacgatgagt ccaaaaaaac ttatcgccgc gctctctttg
     8341 acgaaatcta atttaaatga agaaattctt ccttattacc gccggtatta tcctggtttt
     8401 ggtattgtca tctgtttttt atgtcatctc caacctatct tcggtttcta ctgacactaa
     8461 aactgtcact tttgttatca ataaaggcga cggcattatc aatgtcgccc aaaaactgca
     8521 ctccaaccgt cttatcaaag ataaatattc ttttgttgcc tatgctgtag ctaccaggca
     8581 gaatcaaaaa attccctctg gcaccttcag actttctccc tcactttccg tcaaagaaat
     8641 ccttgtcaat tttgccagcg gcggcatttc tgattattgg ataaaaatca tcgatggttt
     8701 tcgcgtcgaa gaaatcgccc ctcttttccc cgaagactta tcttttactc cgtctgattt
     8761 tcttgccaaa gccaaatcaa aggaaggtca tctttttccc gacagctatc ttcttcccaa
     8821 gtattacaat cttgatcagg ttttagattt ttttgatcaa aatttcacca aaaaaatttc
     8881 tcaggccaaa tcggatagcc acaccaccct ttcagaccgc gataatttaa ttttagcttc
     8941 tttggtagaa agagaaggac ggtcactggc agccaaaaaa atgattgccg gaattttatt
     9001 aaatcgactg aatatcaata tgccgcttca ggtggacgcc tccgttcaat atgctcgtga
     9061 cagccgtagc aaaaatttgg ctaaatattg gcagccggtc acccgcgacg acctcaaaca
     9121 aattgactcc gattacaata cctatcttta ccctaaactg ccgccgggtc caatctgcaa
     9181 tccgggctac gactcaattt atgctgttta tcaccccacc gattctgact atctttacta
     9241 catcaccggc aatgacaacc agatgcatta tgccaaaact ttagaccagc acaatcaaaa
     9301 catcgccaat tatctgcgct agtttttatt aatcatttgg tttaatctta cccatctttc
     9361 tttcttgacc ttcattggca catcgtctgg gtatagtttt tcacttactg tccctggtcg
     9421 gggagagtat ttgttcaaat aagccacctc aaaacccact tgtcggcaga tatcaaccgt
     9481 cttttcaaac gccttgtcac tttctcctgg gaagccaata ataatatccg tgctgaatct
     9541 agctccattc actctagact ttattttttt taccaaatcc aaatactctt gtttagtata
     9601 gtttcggttc atttttttta aaatttcatc atcgcccgcc tgaaatggta aatgaatcag
     9661 tctgtcaata tttttatatt ttgctatcac ttcaataaga tcatccgaaa aatcccacgg
     9721 attattgctt acaaacgaca cttgattcaa atctttttta gccacttctg ccaataaatt
     9781 tggaaacaaa cttttaaatc ttttcttgcc cataaacaca taaccgcagt cgctgagctc
     9841 tgagccatat gaatttacat tttgtccgat tagcaaaatt tttttattcc cttttttgac
     9901 cgcttcatca acatctgcca aaatttcagc cattgatcgg gaaatttctt tgcctttggc
     9961 ttggggcaca atgcagtaag agcacatatt gttgcagcca tagctaatcg gcaccaacac
    10021 cactccttta aattttttaa ttggctcagt atcaattaca tatttatcaa tcgccctaaa
    10081 ttctatgccg ggaaaatttt ttttcgtttc cattttcttt ttgttgtttt tgttcattac
    10141 ccccaccaag caaccggtca ccaccacttt tttacccagt ttttgaacat ttctcaaaaa
    10201 tccataagct cggttctctg ccgattgacg aatgacacag gtattgatta tgacttcatc
    10261 agcctctttc cagtttttga cctcctgcca gccgttttcc caatagtgcg ccttaatcct
    10321 ctccgaatca gacgtatttt gaatgcatcc aaaagttttt ataaaaaatt tcacccttga
    10381 ttttatcaaa tcaaatctac ttggctattt ttttagccgc taacaacaat cccgaaccta
    10441 aagttgacaa cagcactgtc ggcaacacta ctcccaccgt tggtagggta gtcgtgcccg
    10501 catcagcatt aaccgcctcc cagtttacat tttccgcccc cattgtcagc gtaaaattta
    10561 ttgtcgatcc gttgttggaa ataactactg tatgatcgcc tccgctcaag gctccggctg
    10621 aataagacca attattatta ccatcactgg ttgccgtccc ctcacttccg tcaacactta
    10681 ccgtcaccac acttcccgcc ggcgcttcac cattaaaaac tgcatttgtc cccgaatacc
    10741 accaatgtga aatttgtctc ccgccggtat caaccgagcc aattgacttc agattaaaac
    10801 cgctggccaa aacctcgccc ttaaaactaa ccacgcacac caaaaaccac aaaactatca
    10861 atattttttt catcctaatc aaatcttaaa actattattt ttaatttgca actttttaaa
    10921 ataaatgaga ctattatttt atggacgata tcaaaaccaa cccactaact gatcagcagg
    10981 atcaggataa ctatcaagaa attcttgaca aatatgctgc ctccgttaaa cctgaagctg
    11041 aaattgctcc cgctccagaa aatccagccc cggagacggt ttctgccacc cttgttgata
    11101 ctgagacttc tattgataac caaaacatca ctccttctct ggaaataaac caaccggata
    11161 ctaaccccag tgttattgct ccacccgagc cgcctcccga acccaaaact aaaacccctg
    11221 aagaaatcaa agccgaggtt gacaagattt tagccgatag tcctgacaac cacccttctt
    11281 ccgagactgt tattgcgcct ccaactcctt cctccagcaa atttttaaaa accatcttta
    11341 tcatttcttt aattattttt ttggcggttg ttggtttact ggtctatttt atgtttttca
    11401 ataaaccggt ttcaaccggt cctgttgata ataattctgt tcctactgac gttcccgaag
    11461 ttgcttgtga attaaatggc gtcgaataca aattaggtga atcattccct tccgctgatg
    11521 gttgcaacac ttgtgtttgt cagtcccccg atgttatcgc ctgcaccgag agagcctgca
    11581 atactaccag tactctcaac actcaaagca ctaccgttgc gccgacagct accaaatccg
    11641 gcacccaaaa tcccagtcct accaaatctg tccttccgac tttgggtcgt tgatttttaa
    11701 tctaaattct acctattgcc aactcttgac actaggttta taataccttc attctggcga
    11761 gcggttggta cataactcgc gtgtccgtcg aagacatttt gttgacggat tattatcttt
    11821 agtaattaaa ttcattgatg ccttccgtaa aacgaactta ctggggtaaa aagtatccca
    11881 atattccc
//
LOCUS       DAOSHV010000006        11782 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_4595_length_11782_cov_4.853586, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000006 DAOSHV010000000
VERSION     DAOSHV010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 11782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11782
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4595_length_11782_cov_4.853586"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            112..1284
                     /gene="tuf"
                     /locus_tag="PKZ29_00565"
     CDS             112..1284
                     /gene="tuf"
                     /locus_tag="PKZ29_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HOG37396.1"
                     /translation="MADTFQRTKPHVNVGTIGHVDHGKTTLTAAISKIQGSAKSYADI
                     AKGGTVRDASKIVTIAISHIEYETDKRHYAHIDCPGHADYVKNMITGAAQMDGAILVV
                     SAPDGPMPQTREHVLLAKQVNVPKLVVYLNKVDLVEDPEFLDLVEMEIRDLLTKYGYP
                     GDEVPIIRGSALKALNGEADGVESIKKLMEALDTYIPEPVRELDKPFLMEIEDVFSIK
                     GRGTVATGRINRGIVKVNDEVEIVGLRDTRKAVVTGVEMFHKQLDQGQAGDNVGILLR
                     GVEKDDIERGQVLAKPGTITPHTEFEAEVLLLKKEEGGRHTPIFTGYRPQVFVGTTDV
                     TGDISLAEGTERIMPGDNAKVTVKLIKPIAMEDGQRFAIREGGLTVGAGAITKIIK"
     gene            1290..1601
                     /gene="rpsJ"
                     /locus_tag="PKZ29_00570"
     CDS             1290..1601
                     /gene="rpsJ"
                     /locus_tag="PKZ29_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014015734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOG37397.1"
                     /translation="MSKGNRIRIRLKSFDHRIIDEAALKIVEAAVTAGAKVEGPIPLP
                     TEHKTITVLTSPHTDKDAREHFELRTHKRLIDISNTSLRTVDSLQHLDLPSGVEVEVK
                     M"
     gene            1604..2224
                     /gene="rplC"
                     /locus_tag="PKZ29_00575"
     CDS             1604..2224
                     /gene="rplC"
                     /locus_tag="PKZ29_00575"
                     /inference="COORDINATES: protein motif:HMM:TIGR03625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOG37398.1"
                     /translation="MISGFLVKKGHMTSVYSQDGRRVAVTKCLALPLKVTQIKTVEKD
                     GYQSVQVAYGSKKHLDKATGSKISKLKLDIKPRSFKEFKLVSDQIPEIGADITFESVF
                     NQGDIVDITGISRGRGFAGVVKRHGFKKQPILGSSDRVRRPGSIGAQTPGKVVKGKKM
                     PGHMGVETITIPNLEIFSINPDTKEVLIKGSVPGSFNSWITVKKTN"
     gene            2228..2860
                     /gene="rplD"
                     /locus_tag="PKZ29_00580"
     CDS             2228..2860
                     /gene="rplD"
                     /locus_tag="PKZ29_00580"
                     /inference="COORDINATES: protein motif:HMM:TIGR03953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOG37399.1"
                     /translation="MKSQLYNLKSEKIGEIALPKSVFEVKVSDTLISQAIRVTLANRR
                     SSFAKTKTRSEVAGTRKKVWSQKGTGNARHGNRTAPIFVGGGIALGPDGQQNYSLKIG
                     KNMRKKALYALLSKFAAGKKILVIDNFSHLDPKTKSGVKLVAGLKSQDEVLSKSRKIG
                     IITTNTLTPVKRAFGNIKDISLISLKSLNIYDLSKQNYLIFSQKAIRQFK"
     gene            2863..3162
                     /locus_tag="PKZ29_00585"
     CDS             2863..3162
                     /locus_tag="PKZ29_00585"
                     /inference="COORDINATES: protein motif:HMM:NF012498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:1990904 - ribonucleoprotein complex
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOG37400.1"
                     /translation="MNHNIIDLKPVVTEKALSAQQTQDKYAFWVSPRATKGQIEVAFK
                     SVFNIAPLSVNTVTLKGKTKIHWKSRASIQKSDRKKAIISVPKGTKIELLKLNTK"
     gene            3164..3907
                     /gene="rplB"
                     /locus_tag="PKZ29_00590"
     CDS             3164..3907
                     /gene="rplB"
                     /locus_tag="PKZ29_00590"
                     /inference="COORDINATES: protein motif:HMM:TIGR01171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOG37401.1"
                     /translation="MSHKPLLTIKKKHSGRDSRGHVSVRHQGGQQKRFLREIEWNRNR
                     FDVPAKVVSIEYDPNRTSDIALLVYENGAKSYILSTAGLKVGDTVISANQAPIKDGNC
                     LPLKNIPIGVPVHAISFQVGKPAQIVKTAGSAAFIQSIDGTKATVKLPSGEIRIFHSD
                     CRATVGQVSNADHSNQKLTKAGQSRHRGVRPGVRGVAQNPKSHPHGGGEGRSSIGMHP
                     KTKWGRPAFKKTRKLKKASNKLIVQRRKK"
     gene            3925..4197
                     /gene="rpsS"
                     /locus_tag="PKZ29_00595"
     CDS             3925..4197
                     /gene="rpsS"
                     /locus_tag="PKZ29_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOG37402.1"
                     /translation="MSRSSKKGPYIDQNILDKIAKGGSQVIKTYARMSSISPEFVGRT
                     FDVHNGKKFISVYVTEDMVGHKLGEFAPTRTFKGHGRVVKRVIEKT"
     gene            4204..4662
                     /locus_tag="PKZ29_00600"
     CDS             4204..4662
                     /locus_tag="PKZ29_00600"
                     /inference="COORDINATES: protein motif:HMM:NF012462.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0042255 - ribosome assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uL22 family ribosomal protein"
                     /protein_id="HOG37403.1"
                     /translation="MPKITKKTTTAPKKTVKKTVSATKPVKPVKVPAALPVFKTAKYV
                     NKNIQISPRKLRLLVDEVRQFSPVEALNRVKFINTKSARIMVQALKNIIADAKNNFNL
                     DTNSLKFDTIKADEALKIKRMDKSHGSRFQRGLIQKRHSRLVIIVKGVQK"
     gene            4664..5305
                     /gene="rpsC"
                     /locus_tag="PKZ29_00605"
     CDS             4664..5305
                     /gene="rpsC"
                     /locus_tag="PKZ29_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013182553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOG37404.1"
                     /translation="MGQKVNPIGFRLEAVKNSPEWQSSWFANSKKYSVFLLEDKKIRD
                     FLQKRIYSAGIVSIKIERSVKKLKITIIVARPGLVIGRGGKELEALKKEIQKLLNKNA
                     GKQSVEIQVEEFKHADLSAKLVAEKIAYQLTKRMPYRRVVLSAIEKSISSGAKGVKVV
                     LSGRINGAEIGRREKFSEGKIPLSTIRSKIDYYECPSLTRSGYIGVKVYLCLI"
     gene            5351..5752
                     /gene="rplP"
                     /locus_tag="PKZ29_00610"
     CDS             5351..5752
                     /gene="rplP"
                     /locus_tag="PKZ29_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOG37405.1"
                     /translation="MLQPSRTKYRKQFRGKMRGNAKGNLVAFGEYGLKSMECGWLTAN
                     QIEAARRAISNATKRSGRTWIRVFPDKPFTQKGTGTMGAGKGEVKGYVAVVKPGRIMF
                     EISGISPEDSQKVLKLAAAKLPVVCKIVTKK"
     gene            5764..5973
                     /gene="rpmC"
                     /locus_tag="PKZ29_00615"
     CDS             5764..5973
                     /gene="rpmC"
                     /locus_tag="PKZ29_00615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOG37406.1"
                     /translation="MKKTDKIAYRQKSSAELKKSLIDLKKSLIESKSKLATGNQSDTS
                     VFKKTKKQIALILTLLGETKNETKN"
     gene            5957..6217
                     /gene="rpsQ"
                     /locus_tag="PKZ29_00620"
     CDS             5957..6217
                     /gene="rpsQ"
                     /locus_tag="PKZ29_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOG37407.1"
                     /translation="MKQKIKNTGKTFIGNVVSDKMSNTIVVSLEYTRRHPMYKKIIRK
                     HKNIYADNNLSAKIGDIVKVRETKPLSKLKRFTTIEIIKKSI"
     gene            6223..6591
                     /gene="rplN"
                     /locus_tag="PKZ29_00625"
     CDS             6223..6591
                     /gene="rplN"
                     /locus_tag="PKZ29_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015919496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOG37408.1"
                     /translation="MVQLRSIIKPADNCGAKRVQVVHVYKGHFHKKATIGDIVLAVVK
                     DAIPNGMVKKKEKVKMVIVRTKKEFGREDGSYIRFSDNAGVVIDSQNNPRGTRIFGPI
                     AREIKDLGFNKIASMAQEVY"
     gene            6593..6940
                     /gene="rplX"
                     /locus_tag="PKZ29_00630"
     CDS             6593..6940
                     /gene="rplX"
                     /locus_tag="PKZ29_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOG37409.1"
                     /translation="MKILKGDKVKILIGKDKGREGLVIKSFPKKSSLVVKDLNIFKKH
                     VKPTQGKPGSIVEKERPIYISKVALICPECKKTTRVGYKIDKSGEKYRICKKCNSIIV
                     GTAKGISAPAKKK"
     gene            6943..7503
                     /gene="rplE"
                     /locus_tag="PKZ29_00635"
     CDS             6943..7503
                     /gene="rplE"
                     /locus_tag="PKZ29_00635"
                     /inference="COORDINATES: protein motif:HMM:NF000585.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOG37410.1"
                     /translation="MLKDTINTTIKQNLIDKIGKKRNIFSLPHLSKVVINYRVGEARE
                     SQESLASATEEIIAITGQKPQLCKAKKAVSAFKLRQNDPLALKVTLRGARMYDFVEKL
                     FNLILPRLRDFKGMSATAFDSVGNYNLTIKDQTLFPEINLDKVNKIRSVQITLNIKAA
                     SKDEAKMLLSALGFPFEKSADSDSQK"
     gene            7506..7691
                     /locus_tag="PKZ29_00640"
     CDS             7506..7691
                     /locus_tag="PKZ29_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011917778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HOG37411.1"
                     /translation="MATTAKIVRETKKLKYRVRYHNRCSICGRPRGYIRLFGICRLCF
                     RKLAHQGMLPGVKKSSW"
     gene            7696..8085
                     /locus_tag="PKZ29_00645"
     CDS             7696..8085
                     /locus_tag="PKZ29_00645"
                     /inference="COORDINATES: protein motif:HMM:NF012627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOG37412.1"
                     /translation="MLQSTSIDFLIRIKNAVKAGKKNMTAPASNFCVNIADLLKRYGF
                     VTSYSLTDDAKRRISLELSYAGNQPKITDVKIISKPGCRVYEKVSSLPWGKTPNSLII
                     ISTSAGVMSQKEAKTKKLGGEIIAEVY"
     gene            8099..8638
                     /gene="rplF"
                     /locus_tag="PKZ29_00650"
     CDS             8099..8638
                     /gene="rplF"
                     /locus_tag="PKZ29_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017434007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOG37413.1"
                     /translation="MSRIGKQPISIPAGVTATVENNTVTIAGPKGNLSLVLPFKIKVE
                     VKDIQLTVSRLSEDKKTKACHGATRSHLNNMVDGVQNHWQKELEIRGTGYKASLNGNK
                     LKLNVGFIHPVEITAPEGITFQVQEETKITVSGPNRMVVGQIASNIRKIRPPEPYKGK
                     GIRYLNEFIKLKAGKKAKA"
     gene            8641..8985
                     /gene="rplR"
                     /locus_tag="PKZ29_00655"
     CDS             8641..8985
                     /gene="rplR"
                     /locus_tag="PKZ29_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002816352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOG37414.1"
                     /translation="MIEQNKKIRRLNRVRSKVAKNFGIPRLSVFRSNQHIWAQIIDDK
                     HGKTLVSASTKTVKSLKGTKSEKAVAVGQSLAKSALDKKITQVRFDRGSYRYHGRVKA
                     LADGARSGGLKF"
     gene            8987..9469
                     /locus_tag="PKZ29_00660"
     CDS             8987..9469
                     /locus_tag="PKZ29_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006505639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOG37415.1"
                     /translation="MIPNNHKPNQKNDRFEKPVSEFTDKVVQIKRVSKKTKGGNQIHF
                     TALVVSGDKKSRVGVGLGKAKSVADAIQKGIKKAQKSLITIPIIDGTISGSIKLKFKG
                     AVVMLRPGKTGSGIIAGGPVRSVVEAAGIKDIASKIIGSKNKSINVWATLKALSSLQK
                     "
     gene            9471..9920
                     /gene="rplO"
                     /locus_tag="PKZ29_00665"
     CDS             9471..9920
                     /gene="rplO"
                     /locus_tag="PKZ29_00665"
                     /inference="COORDINATES: protein motif:HMM:TIGR01071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOG37416.1"
                     /translation="MEILSQLTKITAKPARRRGRGIGSGLGGHTVGRGAKGDKARGKT
                     KLTFDGTKIKKSWLKRLPFMRGKHRLSNRKNVVIFNLTQIDKWFKKDDLVDLNSLTKK
                     TKISFKNLNASVKILSAGDITKPLKFKGVSVSQSASKKIIAAGGNIE"
     gene            9920..11200
                     /gene="secY"
                     /locus_tag="PKZ29_00670"
     CDS             9920..11200
                     /gene="secY"
                     /locus_tag="PKZ29_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOG37417.1"
                     /translation="MNRYTKTLSLYIEGLKTPILRKKLLYTLGILIAFRFIAHIPVPG
                     VNVAMLRQFFNQNQLLSLLDIFSGGTLANFSITALGLSPYISASVMIQLLALVIPQLA
                     ELQKEGEYGQSKINLYTRIATIPFAIINSLGMYAILRSQNIITTFNLLTTIAVVVSML
                     AGTMIMIFFGELINLYGVGNGISMLIFAGIISRLPISLVQTFSVTDLSNPQTGLNLAI
                     FTGMALVMVAAIVFVEEAILKVPINYAKRGQSTYLPIKVNSAGVMPIIFAMSLATVPS
                     MLGQFLSKVKNPSIAGFAVGMSNFFKTDGYFYMIFYFLLVIGFTFFYTSVVFKPKDIA
                     EELRKSGGFIPGIRPGQSTEKRLQYLINRVLLIGAVFLGIIAVTPSIIQKMTNITTLT
                     IGGTSILIVVSVILELTRKIENVVQTYNYDKLTY"
     gene            11221..>11782
                     /locus_tag="PKZ29_00675"
     CDS             11221..>11782
                     /locus_tag="PKZ29_00675"
                     /inference="COORDINATES: protein motif:HMM:NF012624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HOG37418.1"
                     /translation="MSLNLFIIGPSGCGKSTQAKLIADKYHLTHLSMGQLLRDEMAAA
                     TSEGLEAKSYVDNGNLVPEELSFRVLTKSLEKINNQNFIIDGFPRTLGQGKFIENYLA
                     QKGEPLDLIIHLDVTFEEISARRTKMGIEFQDKNRTDSTPEAITARQKFYDDNNGLIM
                     DYFSPKNLLFRVDGNRPIEPIFIDIDQ"
BASE COUNT         3716 a         2562 c         2179 g         3325 t
ORIGIN      
        1 atattgcaaa gctaacaact agctgataca attctaagac gttttatctt aaaatattat
       61 taattagaaa ttagtcttaa acaagacaat aaattaaaaa aaggagaaaa aatggcagac
      121 acttttcaaa ggaccaagcc gcacgttaac gttggtacta tcggtcacgt cgatcatggt
      181 aaaaccactt taaccgctgc tatttccaaa attcaaggaa gtgccaaatc atacgctgat
      241 atcgccaagg gcggtactgt gcgtgatgct tccaaaattg ttactattgc tatctctcac
      301 atcgagtacg aaaccgacaa gagacattat gctcacatcg attgtcccgg ccacgctgat
      361 tatgtcaaaa acatgattac cggtgctgct caaatggacg gtgctatttt ggtggtctct
      421 gcacccgatg gtcccatgcc tcaaactcgg gaacacgttt tgttagccaa acaggttaat
      481 gttcccaagt tagtcgtcta tctcaataaa gtcgatttag ttgaagatcc tgagttttta
      541 gacctggtcg aaatggaaat ccgtgatttg ttaaccaaat acggttatcc cggtgatgaa
      601 gtcccgatta ttcgcggttc tgctctcaaa gctctcaacg gcgaagccga tggcgttgaa
      661 tctatcaaaa aattaatgga agctcttgat acctacattc ctgagcctgt ccgcgaactc
      721 gacaaaccat ttttaatgga aattgaggat gttttctcca tcaaaggtcg tggcactgtc
      781 gctactggta gaatcaaccg cggtattgtc aaggttaacg atgaagttga aatcgtcggt
      841 cttcgtgata cccgcaaagc tgttgttacc ggagtcgaaa tgttccacaa acagctcgat
      901 caaggtcaag ctggcgacaa cgtcggtatc ttgttgcgcg gtgttgaaaa agacgatatc
      961 gaaagaggtc aggttttagc caaaccaggc accattactc ctcataccga attcgaagct
     1021 gaagttcttt tactcaaaaa ggaagagggt ggtcgtcata cacctatctt taccggttac
     1081 cgaccacaag tctttgtcgg cactaccgat gttaccggtg atattagttt agccgaaggc
     1141 acagaaagga ttatgcctgg tgacaacgcc aaagttaccg ttaagttaat caaacctatc
     1201 gctatggaag atggtcaaag atttgctatc cgcgaaggtg gtttaactgt tggtgccggc
     1261 gctattacca agatcattaa ataataaata tgtccaaagg taaccgtatc cgcatcagat
     1321 tgaagtcgtt tgatcatcgt attatcgatg aggcggctct caagatagtt gaggctgcgg
     1381 ttaccgctgg agccaaagtt gaaggtccaa ttcctttacc caccgaacac aagaccatta
     1441 ctgttttaac cagtcctcat actgacaagg atgctcgtga gcattttgaa ctcagaactc
     1501 acaagcgtct gattgatatt tctaatacca gtcttcgcac tgttgatagt cttcagcatt
     1561 tggatttgcc ctcaggagtg gaagtagaag ttaagatgta actatgattt caggattttt
     1621 agtcaaaaaa ggtcatatga ccagcgttta tagccaagac ggtcgtcggg ttgccgtgac
     1681 taaatgtttg gctctgccgc ttaaagtaac tcaaataaaa accgtcgaaa aagacggtta
     1741 tcaatctgtt caagttgcct atggttccaa aaaacatctt gacaaagcta ctggatcaaa
     1801 aatatccaaa cttaaattgg acattaagcc ccgctctttt aaagagttca aattagtttc
     1861 cgatcaaatc cctgaaatcg gcgctgatat cacttttgaa tcagttttta atcagggaga
     1921 tattgttgac attaccggca tttccagagg tcgtggtttt gccggtgttg tcaaacgaca
     1981 tggtttcaaa aaacagccca tattaggttc ttctgaccgc gtccgccgtc ctggatctat
     2041 cggcgctcaa actcccggca aagttgttaa aggcaaaaaa atgcccggac atatgggagt
     2101 tgaaactatt actattccca atctagaaat tttctccatc aaccctgaca ccaaagaagt
     2161 tttgattaaa ggttcagttc ccggaagttt taattcttgg ataaccgtca aaaaaactaa
     2221 ttaacctatg aaatctcaac tttataatct caaatcagaa aaaatcggcg aaatcgcttt
     2281 acccaaaagt gtttttgaag tcaaagtttc cgatactctt atttctcagg caattagagt
     2341 taccctagcc aatcgccgtt cttcttttgc caaaaccaaa acccgatctg aagttgccgg
     2401 cacccgcaaa aaagtttggt cccaaaaagg caccggtaat gcccgtcacg gcaatcgcac
     2461 tgcccctatt tttgttggcg gcggtatcgc tctcggaccc gatggtcaac aaaactattc
     2521 tcttaaaatc ggcaaaaata tgagaaagaa agctctttat gctcttcttt ccaaatttgc
     2581 tgccggcaaa aagatattag ttattgataa tttttcccat cttgatccca aaaccaaatc
     2641 cggagtcaaa cttgtggctg gacttaaatc ccaagatgaa gtattatcaa aaagtcgtaa
     2701 aatagggatt attactacca acactctcac tcctgtcaag agagcttttg gcaatatcaa
     2761 agatatttct ttaatatcgc tcaaatctct taatatttac gatctgtcaa aacaaaatta
     2821 cttgattttt agccaaaaag ctatcagaca atttaaataa tcatgaatca caatattatt
     2881 gatttaaaac cagttgttac cgaaaaggcc ctatctgccc aacaaacgca ggacaaatat
     2941 gctttttggg tcagcccacg cgctaccaaa ggtcaaatcg aagtcgcttt taaatcagtt
     3001 tttaatattg ctcctttatc tgtcaatacc gttactctca aaggcaaaac caaaattcac
     3061 tggaaatcac gggcttctat ccaaaaatcc gacagaaaaa aagccattat ctctgtccct
     3121 aagggcacca aaattgaatt acttaaacta aataccaaat aatatgtcac acaaaccttt
     3181 attaaccatc aaaaagaaac acagcggtcg tgactctcgc ggtcacgttt ctgtccgcca
     3241 ccaaggtggt cagcaaaaac gttttcttcg cgaaattgaa tggaatcgta atcgctttga
     3301 tgttccagca aaggttgttt ctatcgaata cgatcctaac cgaacttcag atatcgctct
     3361 tttggtttac gaaaacggtg ccaagtccta tattttgtct actgccggtc tcaaagtcgg
     3421 ggatacagtt atttctgcca atcaggctcc catcaaagac ggcaattgtt tgcctctaaa
     3481 aaacattccc attggggtgc ctgttcatgc cattagtttt caggtgggca agccggctca
     3541 aatagtcaag actgccggat cagccgcctt tatccaaagt atcgatggca ccaaagccac
     3601 cgtcaaatta ccttcaggtg aaattagaat tttccattcc gactgtcggg ctactgttgg
     3661 ccaggtcagc aacgccgacc attccaacca aaaattaacc aaggccggac aatcccgtca
     3721 ccgcggtgtc agaccaggtg tcagaggtgt ggctcaaaat cccaaatctc atccgcatgg
     3781 aggtggagaa ggccgcagtt ctattggtat gcatcccaaa accaagtggg gtcgacccgc
     3841 tttcaaaaag acccgcaaac tcaaaaaagc ttccaacaaa ttaatcgttc agcggagaaa
     3901 aaaataataa ctttataatt aattatgtcc agaagctcaa aaaaaggtcc atacatcgat
     3961 caaaacattc ttgacaaaat tgccaaaggt ggcagtcagg taatcaaaac ttatgcccga
     4021 atgagttcca tttctccgga atttgtcggt cggacctttg atgttcataa cggtaaaaaa
     4081 ttcattagtg tctatgttac cgaagatatg gttggacaca agttaggtga atttgctccc
     4141 accaggacct ttaaaggtca tggccgggtg gttaaaagag ttattgaaaa aacctaaaat
     4201 tttatgccaa aaattactaa aaaaactact accgctccca aaaagactgt caaaaaaact
     4261 gtctctgcta ccaaaccagt caagccagtc aaagttccgg ctgccttgcc ggtttttaaa
     4321 accgccaaat atgtcaataa aaatatccag atttcacctc gaaaattacg tttgttggtt
     4381 gatgaggtcc gccaatttag tccggttgaa gctcttaatc gggttaaatt tatcaatacc
     4441 aaatcagcca ggattatggt ccaggctcta aaaaatatta ttgctgacgc caaaaataat
     4501 tttaatttag atacaaactc attaaaattt gatactataa aagccgacga agctttaaaa
     4561 ataaaacgta tggacaaatc acacggctct cgttttcagc gtggtctgat ccaaaaacgt
     4621 catagtcggc tcgtcattat tgttaaagga gtccaaaaat aatatgggtc aaaaagttaa
     4681 tcctatcggt tttagactcg aggctgtcaa gaacagtcct gagtggcaat ccagctggtt
     4741 tgccaattcc aaaaaatatt ctgtttttct tcttgaagac aaaaaaattc gtgacttttt
     4801 gcaaaagagg atttattctg ccggtattgt tagtatcaaa attgaaagat ccgtcaaaaa
     4861 acttaaaatt accatcattg ttgctcgacc gggtttagtt attggtcgtg gcggcaagga
     4921 attggaagct ctcaaaaagg aaattcaaaa acttcttaac aaaaatgctg gcaaacagtc
     4981 agttgaaatc caagtcgaag agtttaaaca tgctgacctt tctgccaaat tagttgccga
     5041 aaaaattgct tatcagctta ccaaaagaat gccttaccgt cgggtggttt tatccgccat
     5101 tgaaaaatca atctcttccg gcgccaaagg tgtcaaagtt gttttatccg gtcgtatcaa
     5161 tggtgccgaa atcggtcgcc gggaaaaatt ttccgaaggc aaaattcctt taagcaccat
     5221 cagatcaaaa attgattatt acgaatgtcc gtcgcttacg cgttccggat atatcggagt
     5281 taaagtttat ttatgtttaa tttaattttt tcaataaact ttacaaactt tataacttta
     5341 taaactactt atgttacaac caagccgaac caaataccga aaacagtttc gcggcaaaat
     5401 gcggggtaat gccaagggta atttagttgc ctttggagaa tacggtctca aaagtatgga
     5461 atgcggttgg ctaactgcca accaaatcga agccgcccga cgggctattt ccaacgccac
     5521 caaaagaagc ggtcggactt ggattagagt ttttcctgac aagcctttta cccaaaaagg
     5581 caccggcacc atgggtgctg gcaaaggcga agttaagggc tatgttgccg ttgttaaacc
     5641 cggtcggatc atgtttgaaa tttccggtat tagtccagaa gactctcaaa aagttctcaa
     5701 acttgccgcc gccaagttac cagttgtctg caaaatcgtt accaaaaaat aggagtggaa
     5761 tttatgaaaa aaactgataa aattgcctac agacaaaaga gttcagcaga gcttaaaaaa
     5821 agcctaattg acctcaaaaa aagtctcatt gagtcgaaat caaagttggc tactggcaac
     5881 cagtccgata cttctgtttt caaaaagacc aaaaaacaaa ttgctctaat tttaaccctt
     5941 ttgggagaaa ccaaaaatga aacaaaaaat taaaaatacc ggcaaaactt ttatcggcaa
     6001 tgttgtctcc gacaagatgt ccaatactat tgtcgtttct cttgaatata cccgccggca
     6061 tcctatgtac aaaaaaatta ttcgcaaaca caaaaatatt tatgccgaca acaatctttc
     6121 tgccaaaatc ggcgatattg tcaaagtccg cgaaaccaaa cccttaagta aattaaaaag
     6181 atttaccact atagaaatta tcaaaaaatc tatctaatta ttatggttca actcagaagt
     6241 atcatcaaac cggctgacaa ctgcggcgcc aaaagagtcc aagttgttca tgtctacaaa
     6301 ggtcattttc acaaaaaagc cactattggt gatattgttt tggctgttgt caaagacgcc
     6361 atccccaacg gcatggtcaa gaagaaagaa aaagtcaaaa tggttattgt ccgcaccaaa
     6421 aaagaatttg gccgcgaaga cggttcctat atccgtttca gtgacaatgc cggagttgtt
     6481 atcgattccc aaaacaatcc tcgcggcacc cgtatttttg gtccaattgc tcgcgaaatc
     6541 aaagatttag gttttaataa aatagcttct atggctcagg aggtttacta atatgaaaat
     6601 actaaaaggt gacaaagtaa aaattcttat cggcaaagac aagggtcgcg aaggcttggt
     6661 tatcaaaagc tttcccaaaa aatccagtct ggtagtcaaa gaccttaata ttttcaaaaa
     6721 acatgtcaag ccgacccaag gcaaacccgg cagtattgtc gaaaaagaac gcccaatcta
     6781 tatctcaaaa gtagctctta tttgtcctga atgcaaaaag accaccagag tcggctacaa
     6841 aatcgacaaa tccggcgaaa aatatcgcat ttgcaaaaag tgcaattcaa ttattgttgg
     6901 caccgccaaa ggcatttccg cccccgccaa aaagaaataa ttatgctaaa agataccatc
     6961 aatactacta tcaaacaaaa tttaattgac aaaatcggca aaaagagaaa tatattttct
     7021 ctgcctcatt tgtcaaaagt tgtcatcaac taccgtgtcg gcgaagcccg cgaaagtcag
     7081 gaatctcttg cttctgccac cgaagaaatt attgctatca ccggtcaaaa accccagcta
     7141 tgcaaggcca aaaaagctgt ttctgccttt aaactccgcc aaaatgatcc cttagctctc
     7201 aaagttactc ttcggggggc aaggatgtat gattttgtcg aaaaattatt caatctgatt
     7261 ttaccccgtc tgcgcgattt taaaggtatg agcgccaccg cttttgacag cgtcggcaat
     7321 tacaatttga ctatcaaaga tcaaaccttg tttcctgaaa tcaatcttga taaagtcaac
     7381 aaaatccgct cagttcaaat aactctaaat atcaaagccg cttccaaaga tgaggcaaaa
     7441 atgttattat cagctctggg ttttcctttc gaaaaatccg cggattctga ttcgcaaaaa
     7501 taattatggc tacaactgca aaaatagtta gagaaacaaa aaaattaaag taccgcgttc
     7561 ggtaccacaa tcgttgttca atttgtggtc gcccccgcgg ttatattcgt ttatttggta
     7621 tttgccgtct ctgcttccgc aagttggctc atcagggcat gcttcctggt gttaaaaaat
     7681 ccagttggta aaaatatgtt gcaatcaact tcaattgatt tcttaattag aataaaaaat
     7741 gctgttaaag ccggcaaaaa aaatatgact gctccggctt caaatttttg cgtcaatatt
     7801 gccgatctcc taaaaagata tggttttgtt accagctatt ctcttactga tgacgctaaa
     7861 cgccgcattt ctttggaatt aagttatgcc ggtaatcaac ccaaaataac cgatgttaaa
     7921 attatttcca aacccggttg tcgggtctac gaaaaagttt cgtccttgcc ttggggtaaa
     7981 acacctaatt ctcttattat tatttctact tccgctgggg ttatgtccca aaaggaagct
     8041 aaaactaaaa aacttggcgg agaaatcatc gccgaagtct attaattaaa ccattattat
     8101 gtcccgcatc ggcaaacaac caatttctat tcccgccgga gtcaccgcca ctgtcgaaaa
     8161 caacaccgtc actattgccg ggccaaaagg aaatctttca ttggtcttgc ctttcaaaat
     8221 caaagtcgaa gttaaagata ttcaattgac cgtcagtcgt ttgtccgaag acaaaaaaac
     8281 caaagcttgt catggcgcca ccagatccca tctcaataat atggttgacg gggttcaaaa
     8341 tcattggcaa aaagagctgg aaatccgcgg cactggttac aaagcctctc ttaacggtaa
     8401 caaactcaaa cttaacgttg gtttcatcca tccggtcgaa atcactgctc ccgaaggtat
     8461 taccttccag gttcaggaag aaactaaaat tactgtttcc ggacccaaca gaatggtggt
     8521 cggtcaaatc gccagcaata tccgcaaaat cagaccaccc gaaccataca aaggcaaggg
     8581 tattcgttac ttaaatgaat ttataaagtt aaaagccggc aagaaagcca aagcttaact
     8641 atgatcgaac aaaataaaaa aattagaaga ttaaatcggg tcagaagtaa ggttgccaaa
     8701 aactttggta ttcctcggtt gtcagttttc cgcagcaacc agcatatctg ggctcagata
     8761 attgacgaca aacacggcaa aactcttgta tcagcttcta ccaaaaccgt caaaagtctc
     8821 aaaggcacca agtctgaaaa ggctgttgct gtcggtcaat ctttggccaa atcagctctc
     8881 gacaaaaaaa ttactcaagt tagatttgat cggggtagct accgttatca cggtcgggtt
     8941 aaagcccttg ccgacggtgc tcgatcagga ggtttgaagt tttaatatga ttccaaataa
     9001 ccataaacca aatcaaaaaa atgaccggtt tgaaaaacca gttagcgaat tcaccgataa
     9061 agtagttcaa atcaaacgtg tctccaaaaa aactaaaggc ggcaatcaaa ttcattttac
     9121 tgccttagtt gtctccggtg acaaaaaatc acgggttggc gttggattgg gcaaagccaa
     9181 aagtgttgct gatgctatcc aaaaaggtat caaaaaagct caaaaatctt taattaccat
     9241 tcccattatt gacggcacta tttccggatc aatcaaatta aaattcaaag gagctgtagt
     9301 tatgcttcgc cccggtaaaa ccggaagcgg tattattgcc ggtggtcccg tccgatcagt
     9361 agtcgaagct gccggtatca aagatattgc cagcaaaatc atcggttcca aaaacaaatc
     9421 gattaatgtt tgggctacct taaaagctct tagttctctc caaaaataat atggaaatac
     9481 tatctcaatt aaccaaaatt actgccaaac ccgcccgtcg tcgtggacgc ggcattggtt
     9541 ctggacttgg tggacacacc gttggccgcg gcgccaaagg ggacaaagcc agaggtaaaa
     9601 ccaaactaac ctttgatggc accaagatca aaaaatcatg gctaaaaaga ttgcctttta
     9661 tgcgtggtaa acaccgactc agcaaccgca aaaatgttgt tatctttaac ttaacccaaa
     9721 ttgataagtg gttcaaaaaa gacgatttag tcgatcttaa ttctctcacc aaaaagacca
     9781 aaatctcttt taaaaatctc aacgcctccg tcaaaattct ttctgccggc gatattacca
     9841 agcctctaaa atttaaagga gtctctgttt ctcaatctgc ttccaaaaaa attattgctg
     9901 cgggtggtaa tatagagtaa tgaatcggta taccaaaacc ctatcacttt atattgaagg
     9961 cctaaaaacc ccaattttac gtaaaaaact gctttatact ctcggaattt taattgcctt
    10021 tagatttatt gcccatattc ctgttcctgg cgtcaatgtt gccatgctac gccagttttt
    10081 taaccaaaat caacttttat cgcttttgga tattttctct ggtggcacct tagctaattt
    10141 ttctattact gctcttggtc ttagtcctta catcagcgct tctgttatga tccagctttt
    10201 ggctttagtt attcctcagc ttgccgaact tcaaaaagaa ggtgagtatg gtcaatcgaa
    10261 aataaatctt tatacccgca ttgctaccat cccttttgct attatcaaca gcttgggtat
    10321 gtatgctatt ctccggtctc aaaatataat caccaccttc aacctgttaa ctactattgc
    10381 cgttgttgtc agcatgttgg caggcactat gattatgatt ttttttggcg agcttattaa
    10441 tctctatgga gttggcaacg gtatatccat gttgattttt gccggtatta tttcccgtct
    10501 gcccatatct ttggttcaaa cattttctgt caccgatctt tccaaccctc aaaccggact
    10561 taatttagct atttttaccg gcatggcgtt ggttatggtg gctgccattg tttttgtcga
    10621 ggaagccatt ctcaaagtcc ccatcaatta tgccaaaaga ggtcagtcaa cttatttgcc
    10681 catcaaggtc aatagcgccg gagttatgcc cattattttt gccatgtcgt tggcaacagt
    10741 tccttctatg ttgggtcagt ttttatccaa agtcaaaaat ccctccattg ccggatttgc
    10801 tgttggtatg tcaaattttt tcaaaactga cggctacttt tatatgatat tttatttcct
    10861 tttggttatc ggctttacct ttttttatac ttcagttgtt tttaaaccaa aagatattgc
    10921 tgaggaactg cgaaaatcag gcggatttat ccccggtatt cgtcccggtc aatcgaccga
    10981 gaaacggctt caatacctca tcaaccgggt tttgctcatt ggagctgtct ttttgggtat
    11041 tattgccgtc accccgtcaa ttatccaaaa aatgactaat attaccactt taaccatcgg
    11101 tggcaccagt attctgatcg ttgtttctgt tattttggag ctaactagaa aaattgaaaa
    11161 tgttgttcaa acttacaatt acgataaact cacttattaa ttaaagttaa atccttaaat
    11221 atgtcattaa atttatttat catcggcccg tcaggttgtg gcaaatcaac tcaagccaaa
    11281 ctgattgccg acaaatatca tctcactcat ttatctatgg gtcagctact tcgtgatgaa
    11341 atggccgccg ctacctccga aggtcttgaa gccaaatctt acgttgacaa cggtaattta
    11401 gttcccgaag aattgtcttt ccgggtttta accaaatctt tagaaaaaat taataatcag
    11461 aattttatta ttgatggttt tccccggact cttggtcaag gtaaatttat cgaaaattat
    11521 ttagcccaaa aaggtgaacc gctggattta atcattcatc ttgatgttac tttcgaagaa
    11581 atcagcgccc gccgtaccaa aatgggaatc gaatttcagg acaaaaaccg caccgactct
    11641 actcccgaag ccattaccgc ccgtcaaaaa ttttacgatg acaacaacgg tttgattatg
    11701 gattattttt cgcccaaaaa tttactgttt agagtcgatg gcaaccgtcc tatcgaacct
    11761 atttttatcg atattgatca aa
//
LOCUS       DAOSHV010000007        11151 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_4926_length_11151_cov_6.312275, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000007 DAOSHV010000000
VERSION     DAOSHV010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 11151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11151
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4926_length_11151_cov_6.312275"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..304)
                     /locus_tag="PKZ29_00680"
     CDS             complement(17..304)
                     /locus_tag="PKZ29_00680"
                     /inference="COORDINATES: protein motif:HMM:NF024061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNHCP domain-containing protein"
                     /protein_id="HOG37419.1"
                     /translation="MKKVVEDFVCEVCGEKVKGDGTTDHCPKCLWGKHVDEETPGDRA
                     SGCRGLMRPIRSEYKNGQFRIYYKCERCNHSFWVREGEGDNREMLVELSGF"
     gene            complement(309..3254)
                     /locus_tag="PKZ29_00685"
     CDS             complement(309..3254)
                     /locus_tag="PKZ29_00685"
                     /inference="COORDINATES: protein motif:HMM:NF024594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4012 domain-containing protein"
                     /protein_id="HOG37420.1"
                     /translation="MGTGLPSALILGIDHFAAKKLAQELVNKDIQVVGVGELVTGWGE
                     LDNFEWKESVDEVEGEFNYVFDFGEEKRIWQRIKADKFTLISVNNEVRSNYLAREVTG
                     WESDWRVVDAREVYGEGMEENSWLGKALVRAVTNKNLELPGPEERVRLLAVDDLVEAI
                     LRACFLSGTEGEYFLVLGKEMKIEEMAKVLMDKAKMTRFKVMEVSKKIDQGNEKLAEA
                     SESQLRWKAETPFEEGVVGTLQYFFSRVDEENRRSKKSKTTVIKPVKELRQQPFAVVV
                     EEKEIEVEGQEEEEILKNDKKGIVEVKMTVFKEEEVGDEFEIPRLIESKQKEAEKVKE
                     DDYIEEESEELTVAERGEPEETVLETNQVKAKKKGGLRWWWGVGVGLILLSLIWPVRW
                     IWSAQSTIKAITKAPELIRDKKYNQVEVTADMKIKELSAIDEKITEWGLNSLETVRNY
                     QTGIKVLIDFLVMEKQSLNLIRTADEMSEAVFGDKEINWEDQLTAINGGLVETENNLG
                     ILQARLSGGSGWVPKNWRGTVQKGLKTIEDLKGQLGLGKEILKIIPEILGLDGRRREY
                     MVLFQNESEIRPTGGFIGSYGLISFQKGRLIDFEVKDIYEADGQLKGHVEPPWEIKAH
                     LGEANWYMRDANWNPDFVKTSADIQWFLEKETQKRVDGVVGIDLAVVRAMLGVTGPIK
                     VTDFKETIDKDNLYEQAEFYAETKFFPGSGQKASFLGTLGKQLFEEIKLLKTEKKMEL
                     YQVLMEMLESNEMQMAINNTQAAKVINEMGWDGKIYNGKCAGEACVTDYWYAVEANVG
                     VNKANYFLRKNIEEGVEFTGESLNRILKINYENTAKNNNWPGGDYKNYLRIYLPKEVV
                     LSQISVSDGYDTTIKKVYSGEEIRIREVEGKKEIGFLVIVPVTKKRIVEIRYSSKISL
                     GDKKEFTYMKYIQKQPGTGETGLVSLVSFGEEWQPIQVEPAASLVGGKLLFNQKLSKD
                     IKMGVVLGK"
     gene            complement(3579..4796)
                     /locus_tag="PKZ29_00690"
     CDS             complement(3579..4796)
                     /locus_tag="PKZ29_00690"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HOG37421.1"
                     /translation="MLKKPIINTGFQILGKIVTVGLSLVTTAVLTRKLGSEVYGNYLL
                     ITSVWLLLDAAADFGSRVMGVREAAAVGDRDKKNRIYVQVAWFRLIASLTGLIVGLGI
                     IWWWPGFEGIEMEALVALLMIGLTATAGSLEIIFQTEMKMGFKVMMDVLFPLVFAAAL
                     MMAGEVNLLWVSGVYLAARAISLGVGLGVVKKVTGKIKLSGPDGNYIKKFLKLTWPMG
                     LYMVLFSGYDRAVDSALIKQLIGAKDLAFYGLAYKIYGNLIQPAYFLINSVFPIMSGK
                     EGNKKRLFWQTAGLMLSGAVVVGAGTYFLAPWVVGILGGQDYKPAVTVLRILMPAMAA
                     AYVGHLVGFTLIARGKQKQILVVGIISLVINIVGNLIFIPRLGINGAAWVTVATEVGA
                     SLLMVGLLRRDIR"
     gene            complement(4818..6053)
                     /locus_tag="PKZ29_00695"
     CDS             complement(4818..6053)
                     /locus_tag="PKZ29_00695"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOG37422.1"
                     /translation="MKDWGEKIRKIILFWLLLLIPTQLGRHFWFDWSLVWGIRVDYFS
                     PTIYMVDILFLGLWLIEVYREGINWRLIKKNWWIVGLVVINLLIAGNKWVAGYRWLRW
                     MQLIWFGGYVIKNKRLVETYLGRIIPYWLITEGFLALGQIAKGGSLNGVWWWLGERRF
                     DFSTIGVAQMSVANVGLVRAYGTFSHPNSLAGFLLVSLLWWLKYYRQLFLITWGKLKW
                     WVVFWSGLLGILLSGSRTIWILTLGAILIWWRFNYRNSKEKLAFWLLVGAGLFLGLEM
                     IDFNYPLANFLSGWDENGMIKRGQLNLAAVGMIKSSPWFGVGLGNFLVKLPEFQKNNQ
                     IFWFQPVHNILLLLVSETGLLGLAGVVWGLRKKINWKSWSKLTWLILGVIGVSGMGDH
                     YWLTLPQNMWLLTLVIALI"
     gene            complement(6344..6817)
                     /locus_tag="PKZ29_00700"
     CDS             complement(6344..6817)
                     /locus_tag="PKZ29_00700"
                     /inference="COORDINATES: protein motif:HMM:NF013583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HOG37423.1"
                     /translation="MDNKKRNEKILKITCDFLGEINFEVEKAFVEDVEGEENQVLVGV
                     TVANPAGLIGFKGKNLVSIQLILGLMVRNQVGENIRVLLDVNNYRSEQRERLDKTVRG
                     LAEKCLLSHRNVALANMSSYERRICHMIVAEINGVVSESEGEGEERHVVIKPKDE"
     gene            complement(6846..7619)
                     /locus_tag="PKZ29_00705"
     CDS             complement(6846..7619)
                     /locus_tag="PKZ29_00705"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HOG37424.1"
                     /translation="MNLFIAPFVNALFGFYYLLGNFGWSIVVVTVIIKLILLPLILPS
                     LKSAKKIQGLQPKLKSLKEKYGSDKQALAKAQMELYKQEGINPMSGCLPQILQIVVLL
                     LFFSAFNMVVNFAQGKGSIEEINSQLIRPFKINEEFKFDLGFLGGDLAQTPAKIFGQG
                     WNVGVVLPIVLLLGSGVLQYLGAKATMPNPKVAENVAKKTEDKEDDMMAAMRTQSLYM
                     MPAMTIFLGWNFSLGILLYWFTNSAMMVAQQILVAKSGK"
     gene            complement(7631..7834)
                     /gene="yidD"
                     /locus_tag="PKZ29_00710"
     CDS             complement(7631..7834)
                     /gene="yidD"
                     /locus_tag="PKZ29_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOG37425.1"
                     /translation="MRRLVLKLLVWYKKYISRGENCRFIPSCSEYTYEAVKKYGVVKG
                     LGLGWKRIWRCRPGGKSGIDLLK"
     gene            complement(7834..8172)
                     /gene="rnpA"
                     /locus_tag="PKZ29_00715"
     CDS             complement(7834..8172)
                     /gene="rnpA"
                     /locus_tag="PKZ29_00715"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOG37426.1"
                     /translation="MIKKINRFSGRSEFLEVKKAGVGVFNSPLFGVLALVKEDKEVRL
                     GWIISKKISKKAVERNKIKRRLSQVLGPRIGKLKPGARIIFLMKKEILGKSLAEIEKE
                     VDKLVVKLEG"
     gene            complement(8216..8353)
                     /gene="rpmH"
                     /locus_tag="PKZ29_00720"
     CDS             complement(8216..8353)
                     /gene="rpmH"
                     /locus_tag="PKZ29_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014245960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOG37427.1"
                     /translation="MTKRTYQPKSKKRSRVHGFLKRMKSVTGRNVLKRRKNKGRKKIT
                     V"
     gene            8528..9862
                     /gene="dnaA"
                     /locus_tag="PKZ29_00725"
     CDS             8528..9862
                     /gene="dnaA"
                     /locus_tag="PKZ29_00725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOG37428.1"
                     /translation="MDIDRLWKNTQEELKVVLAPAVFQTFVSKTELISLQDNKAILAC
                     PNSYLVDINKKRYYQIFKSALDNQTKTQNTLEFIIQPPLATPSPLNSPLFEYTPKNNS
                     PQNNLHPKFTFDTLIVGNSNNFAYAAAQGIIKNPGLSYNPLFIWGGVGVGKTHLMQAV
                     GHELLKTNPQFKIKYFPAETFSNELVSSLKSKTMNQFKDKYRNLDCILVDDVQFLAGK
                     EYTQEEFFHTFNALHMKGKQIILTSDRKPSEIDHLEDRLVSRFMGGLTVDIQLPDYEM
                     RVAIIKQKTEEFQIQITPEAISFLAANIESNIREIEGKLQQILVQSSSQKIIPIDLTF
                     VQNFFNPDQNLNQFSTAKINPRHIISVCAKTFNIKTSDLCGKSRKKDLVFARHLAAYI
                     LITEANLPLEEVGRLLGGRDHTSIMHARDKIHQDLSTNPQISKLINQIKSSF"
     gene            10137..>11151
                     /gene="dnaN"
                     /locus_tag="PKZ29_00730"
     CDS             10137..>11151
                     /gene="dnaN"
                     /locus_tag="PKZ29_00730"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOG37429.1"
                     /translation="MKLSLLQENLNLALTNVSRFVSSKNQLPILGNILISTDQGRLKL
                     SATNLELSINYWIGAKIDQEGSITLPSKEITEFISYLPAGKIDLSLKDNNLFSVSSTK
                     IESDFTTSPATDFPEFPKINPDTVFEIDLDILSQAISQMAFSAATDDTRPILTGILCS
                     FSQNSLTLVSTDGFRLSFKEIKLVNSLNLKTDKSVTFLIPARSLIEVTKLAKTNQKVK
                     IGLTLDEHQVMFVLDDIELVSRLIEGEFPDYKKLIPESYQTKIFINRDELFQSVKLAS
                     VFARESANVVKMNIKNNSVELTANAPQVGQNRIEIETKTEGENLEVAFNYKFLSDFLN
                     ICKS"
BASE COUNT         3213 a         3113 c         1175 g         3650 t
ORIGIN      
        1 atttactccg cactcactaa aacccgctca gctccaccaa catttctcga ttatccccct
       61 ctccttctct cacccaaaaa ctatgattac atctttcaca tttgtaataa attctaaatt
      121 gaccattttt atactcgctt cttatcggcc tcatcaatcc tcgacagccg ctcgccctgt
      181 ctcctggggt ttcctcatcc acatgttttc cccacaaaca cttcggacaa tgatcagttg
      241 tcccatcacc ttttactttt tctccgcaca cttcgcagac aaaatcctcc accactttct
      301 tcataaattt atttccctaa cactaccccc atttttatat ccttgcttag tttttggtta
      361 aataataatt ttccccccac caaactcgcc gccggctcga cctgtattgg ttgccattct
      421 tctccaaacg atactaaact caccaaccct gtttctcccg tccccggctg cttttgaata
      481 tacttcatat aagtaaactc ttttttatct cccaaactga ttttgctact atatcttatt
      541 tctactattc tcttttttgt caccggcact attaccaaaa atccaatttc ttttttacct
      601 tccacctccc tgattctgat ttcctccccg ctataaacct ttttaattgt cgtatcataa
      661 ccatctgaca cgcttatctg cgacaacacc acttcctttg gcaaataaat ccttaaataa
      721 tttttataat cccctcccgg ccaattattg ttcttagccg tattttcata atttattttt
      781 aatattcgat ttaaagactc gccggtaaat tctaccccct cttctatgtt ttttctcaaa
      841 aaataatttg ccttatttac ccctacattt gcttccaccg cataccaata atctgtcacg
      901 cacgcctcgc ccgcgcactt gccattataa atttttccat cccaacccat ttcgtttatc
      961 actttagctg cttgggtgtt atttattgcc atttgcattt cgttactctc caacatttcc
     1021 attaatacct gatacaactc cattttcttt tctgttttta gcagcttaat ctcttcaaac
     1081 aattgtttcc ccagcgttcc caaaaaactt gctttttggc ccgaaccggg aaaaaattta
     1141 gtttcagcat aaaattccgc ctgttcatat aaattgtcct tatcaattgt ctccttaaaa
     1201 tcagttacct ttattggtcc agtaaccccc aacattgccc gcaccaccgc caaatcaatt
     1261 cccaccaccc catctactct tttttgggtt tctttttcca aaaaccactg aatatcagcg
     1321 ctggttttta caaaatccgg attccagtta gcatctctca tgtaccagtt agcctctccc
     1381 aaatgagcct taatttccca cggtggctct acatgaccct taagttgacc atcagcttca
     1441 taaatatcct tcacctcaaa atctatcaat cttccttttt gaaaactaat caaaccatag
     1501 ctgccgataa acccccccgt tggccttatt tccgactcat tttgaaacaa caccatatat
     1561 tcccgcctcc tcccatccaa gcccaaaatt tccggaatta tttttaatat ctcttttccc
     1621 aaacccaatt gtcctttcaa atcctcaatt gtcttcaatc ctttttgcac cgtcccccgc
     1681 caatttttcg gcacccatcc cgaccctccc gaaagtcttg cttgtaaaat tcccaaatta
     1741 ttttccgtct cgaccaaacc cccgtttatt gccgtcaact gatcctccca attaatttcc
     1801 ttatctccaa ataccgcctc actcatttca tctgcagtcc taatcaaatt caatgattgt
     1861 ttttccatta ccaaaaaatc tattaacact ttaattcctg tttgataatt cctcaccgtt
     1921 tccaaactat ttaatcccca ttccgtaatt ttctcatcta ttgccgataa ttcttttatt
     1981 ttcatatccg ctgttacctc cacttggtta tattttttat ctctaatcag ctctggcgct
     2041 ttagtaattg cctttattgt cgactgggcc gaccatatcc atcttaccgg ccaaattaat
     2101 gataataata tcaatcccac ccctactccc caccaccacc ttaatccgcc tttctttttt
     2161 gccttaactt gattggtttc tagtaccgtc tcttccggct cccctctttc tgccactgtc
     2221 agctcctcac tttcttcctc aatataatca tcctccttca ctttctctgc ctctttttgt
     2281 ttgctttcaa tcaatctggg aatttcaaat tcatccccta cttcttcttc cttaaatacc
     2341 gtcattttta cttctactat ccccttttta tcatttttta atatttcttc ctcttcctgc
     2401 ccttctactt ctatctcttt ctcctctact accaccgcaa acggctgctg ccgtaactct
     2461 tttactggtt ttattaccgt agtcttactt tttttgcttc gcctgttttc ctcatccacc
     2521 ctggaaaaaa aatattgcag cgtccccacc actccttcct caaaaggtgt ttctgccttc
     2581 catctcaatt gagactcgct tgcttccgcc aacttttcat ttccttgatc aatttttttt
     2641 gatacttcca tcactttaaa tcgtgtcatt ttagccttgt ccatcaatac ttttgccatc
     2701 tcttcaatct tcatttcttt tcccaatacc aaaaaatact ctccctccgt ccccgacaaa
     2761 aaacacgctc ttaaaattgc ttccaccaaa tcatccaccg ccaacaatct caccctctcc
     2821 tccgggcccg gcagctccaa atttttattt gtcaccgctc gcaccaatgc ttttcccagc
     2881 caactatttt cctccattcc ttccccataa acttctctgg catccactac ccgccaatca
     2941 ctctcccacc ccgttacttc tctggccaaa taattacttc taacctcatt attaaccgat
     3001 atcaatgtaa atttatctgc ttttatcctc tgccatattc ttttttcttc cccaaaatca
     3061 aatacataat taaattcgcc ttcaacttca tctacactct ccttccactc aaaattatcc
     3121 aactctcccc accccgttac taattctcct actcccacta cttgtatatc tttatttacc
     3181 aattcttggg ctaatttttt agccgcaaaa tggtcaatcc ccaatattaa tgccgacggc
     3241 aatcctgtac ccatatatca attttaaact aaatctattc aatctacgac tttagttgtt
     3301 cttgtttttt ctgcggtata ttttcggtta ttttacggta attattttat aaatcagttc
     3361 tgactatatc aaaatgggtg gttaaaacgt gaaaaatgtc ataactaaat aagttagaaa
     3421 tgtaatatgt ctgaactaaa aaattaatct gtttatctca tttccaacct aaattttact
     3481 tttattcttt taaataaatg ttctgattct ggtttttaac cgccaaaaat actcccaaat
     3541 atgttctgat ttacaaccta aaaatatctc aaaaatcttt atctaatgtc tcttcttaaa
     3601 agtcccacca tcaacaaact tgctcccact tccgttgcca ctgttaccca ggctgctccg
     3661 tttatcccca accttggtat aaatattaaa ttacccacaa tgtttattac caaagaaatt
     3721 attcctacca ctaatatctg tttttgttta cctcgggcaa ttagggtaaa tcccaccaaa
     3781 tgacctacat aggctgctgc cattgccggc attaaaattc tcaacactgt caccgccggt
     3841 ttatagtcct gtcctcccag tattcctacc acccaaggtg ccaggaaata agttcccgct
     3901 cccaccacta ctgcccccga caacatcaat ccggctgttt gccaaaacaa tctcttttta
     3961 ttgccttcct tgcccgacat tatcggaaac acgctgttaa tcaaaaaata agccggctga
     4021 atcaaattgc cataaatttt gtatgccaaa ccgtaaaaag ctaaatcctt tgccccaatt
     4081 aactgtttaa tcaaagccga atctaccgcc cgatcatatc ccgaaaacag caccatatac
     4141 aatcccattg gccaagttag ttttaaaaac ttttttatat aatttccatc cggaccggat
     4201 agttttattt ttcccgttac cttttttacc acccctaacc ccactcccaa acttatggcc
     4261 ctggccgcca aatacactcc actcacccac aataaattta cttctcctgc catcatcaac
     4321 gccgctgcaa acactaaagg aaataataca tccatcatta ctttaaaacc cattttcatt
     4381 tcagtctgaa agatgatttc caaacttcct gccgtcgccg tcaatccaat cattagcaat
     4441 gccaccaaag cctccatctc aattccctca aaccccggcc accaccagat tatccccaaa
     4501 ccaactatta aaccagtcaa agatgctatc aacctaaacc atgccacctg aacataaatt
     4561 cggttttttt tatctctatc tcctaccgcc gccgcttctc ttacccccat cacccggctg
     4621 ccaaaatcag ccgccgcatc caacaacaac cacactgaag taatcaacaa ataatttcca
     4681 taaacttctg accccaattt tcttgtcaac accgccgttg ttaccaaact taaacccacc
     4741 gttactattt tccccaatat ctgaaatccg gtattaataa tcggcttttt taacataaat
     4801 tattatctca cctctggcta aattaaagct atcaccagtg tcaacaacca catattctgt
     4861 ggcaaagtca accaataatg atctcccatc ccactaaccc caatcactcc caatattagc
     4921 caggtcaact tactccagct tttccaattt atctttttcc tcaatcccca aactactccc
     4981 gccaacccca acaatcctgt ttccgacacc aacaacagca atatattatg aaccggctga
     5041 aaccaaaaaa tctggttatt tttttgaaat tccggcagtt taactaaaaa atttcccaat
     5101 cctaccccaa accaaggcga acttttaatc atccctaccg ctgccaaatt taactgacct
     5161 cttttaatca tcccgttttc gtcccagccg gacaaaaaat ttgccaacgg ataattaaaa
     5221 tcaatcattt ccaatcccaa aaacaatccc gctcccacca acagccaaaa agctaacttt
     5281 tctttgctat tccgataatt aaatctccac catatcaata ttgcccccaa agtcagtatc
     5341 caaattgtcc gactccccga caataatatc cccagcaacc ccgaccaaaa taccacccac
     5401 cattttaatt ttccccaagt tatcaaaaac aactgccgat aatacttcaa ccaccacaac
     5461 agacttacca gcaaaaaccc cgccaaacta ttaggatgag aaaaggttcc gtatgcccgc
     5521 accaagccca catttgccac cgacatctgc gctactccaa tagtgctaaa atcaaatctc
     5581 ctttctccca accaccacca tactccgttc aaacttcccc ccttagctat ctgacccaac
     5641 gccaaaaatc cttctgttat caaccagtag gggattattc ttcccaaata agtctccacc
     5701 aacctcttgt ttttaattac ataacctcca aaccaaatta attgcatcca tctcaaccac
     5761 cgatatcccg ccacccattt attgccagct atcaacaaat taatcaccac caaacctact
     5821 atccaccagt tttttttaat taatctccaa tttattcctt cccgatatac ctctatcagc
     5881 cacaaaccca aaaacaaaat atccaccata taaattgttg gcgaaaaata atctactctt
     5941 attccccaca ccaaactcca atcaaaccaa aaatgtcttc ccagctgggt tggtatcaat
     6001 aataatagcc aaaataggat tatctttctt attttctccc cccaatcttt cacaccccaa
     6061 tcttaccaca accacttttc accactatta ttattcacaa accccaaatc tttcctgtga
     6121 tctgacatct gagatctgac taactgacat ctatttaact caatccctct aaatctcctt
     6181 ttgacaaaga atacttactg tctcaatcaa cccgaaatca taatttttaa gatttttaag
     6241 tctaaatttc aagtcatagc gccgaaattt tgcctaaaaa tctaatcctg aagagacggc
     6301 agtcgaatcg ggatgaactt gcaacttgat gaacttgaca aacttattca tcttttggtt
     6361 ttatcaccac atgccgctct tctccctctc cctccgattc acttaccact ccatttatct
     6421 ccgctactat catgtggcaa attcttctct cataagagct catgtttgcc aaagctacat
     6481 tcctatggct cagtaaacac ttttctgcca accctctgac agttttatcc aatctctccc
     6541 gttgttccga tcgataatta tttacatcca ataaaactct aatattttct cccacctgat
     6601 ttctcaccat caatcccaaa atcaattgaa ttgatactaa attctttccc ttaaatccaa
     6661 tcaatcctgc tggattagct accgtcaccc ccaccaacac ttgattttcc tcgccttcaa
     6721 catcttctac aaatgccttt tccacctcaa aatttatttc tcctaaaaaa tcacaagtaa
     6781 tttttaagat tttttcgttt ctttttttat tgtccatagt cgctatttta acataatcaa
     6841 ctatcttatt ttcccgactt ggccaccaat atctgttggg ctaccatcat tgccgaattt
     6901 gtaaaccaat acaacaaaat tcccaaagaa aaattccaac ccaaaaatat cgtcattgcc
     6961 ggcatcatat acagcgattg ggttctcatc gccgccatca tatcatcctc tttatcctct
     7021 gtttttttgg ctacattctc tgctactttt ggatttggca tcgttgcctt agctcccaaa
     7081 tattgcaaca ctcccgaacc caacaacagc actattggca acaccacccc cacattccat
     7141 ccctgaccaa aaattttagc cggagtctgc gccaaatctc ctcccaaaaa acccaaatca
     7201 aacttaaact cttcattgat tttaaatggt ctaattaact ggctgttaat ttcctctata
     7261 cttcctttac cttgagcaaa attaaccacc atattaaatg ctgaaaagaa caacaatagc
     7321 accactatct gcaaaatctg tggcaaacac cctgacatcg gattaattcc ttcctgctta
     7381 tacaattcca tttgtgcttt agccaaagct tgtttatcgc tgccgtattt ttctttcaga
     7441 gattttaatt tgggttgtag tccctgaatc tttttggctg atttcaaact tggcaaaatc
     7501 aacggcaaca aaatcaattt gattatcacc gttaccacta caatcgacca gccaaaattc
     7561 cccagcaaat aataaaaccc aaatagggca ttaacaaatg gtgctataaa taagttcata
     7621 aaaagtattt ctattttagc aaatctattc cgctttttcc ccccggacgg caccgccaaa
     7681 ttcttttcca tcccaaaccc aaacctttta ccactccata ttttttcact gcctcataag
     7741 tatattccga acaactggga ataaatcgac aattttctcc tcgggaaata tattttttat
     7801 accaaaccaa aagcttcaac actaatcgcc tcattaacct tccagcttaa ccaccaattt
     7861 atcaacttct ttttctatct ctgccaaact tttgcccaaa atttcttttt tcatcaaaaa
     7921 aattattctt gctcctggtt ttaatttacc aatccttggt cccaaaacct gacttaatcg
     7981 ccgttttatt ttgtttctct ctactgcctt tttagaaatt tttttagaaa ttatccaccc
     8041 cagccttact tctttatcct ctttaaccaa agctaatacc ccaaacaaag gtgagttaaa
     8101 cactcctacc cccgcctttt tcacctccaa aaattcactc ctgcccgaaa atctgtttat
     8161 ctttttaatc actacctatt atagccccaa acttatttga cccgccaact ccaggctaaa
     8221 ccgtaatctt ttttcggcct ttatttttcc ttctcttcaa tacattcctt ccggtcacgc
     8281 ttttcatcct tttcaaaaat ccgtgaaccc gactgcgttt tttacttttt ggttgatatg
     8341 ttctttttgt cataagtcaa attttaccac aaaaaccatt tccttggcta gataacgcct
     8401 aatctgatta ttagtccctt ttacctcttt ccttttgtgg aaaactcatt tattattatg
     8461 tggataactt aaatttcctc cccgaaatta attctctttt gcccccttat tagttatttt
     8521 taatttaatg gatattgacc gcctttggaa aaatactcaa gaggaactta aagtagttct
     8581 cgccccagct gtgtttcaaa cctttgtcag caaaaccgaa cttattagtc tccaagacaa
     8641 caaagccatc cttgcctgtc ccaattctta cttagttgac atcaacaaaa aacgttatta
     8701 tcagattttc aaaagtgctc tagacaacca aaccaaaact caaaatactc tcgaatttat
     8761 tattcaacca cctcttgcca ccccctcacc tctcaactca cctttgtttg aatacacccc
     8821 caaaaacaat tctccccaaa ataatctcca tcccaagttt acttttgaca ccctaattgt
     8881 cggcaacagc aacaattttg cctatgccgc cgcccaaggc attatcaaaa atcccggtct
     8941 ttcctacaac cctcttttta tctggggtgg ggtaggggtc ggtaaaaccc atctcatgca
     9001 ggctgttggt catgaactac taaaaaccaa tcctcaattc aaaatcaaat attttcccgc
     9061 cgaaactttt agcaacgagc tagtttcttc tctcaaatca aaaactatga atcagtttaa
     9121 agacaaatac cgcaatttag actgtatttt ggttgacgat gtccagtttt tagccggcaa
     9181 ggaatacacc caagaagaat tttttcacac cttcaacgct ctccatatga aaggtaagca
     9241 aattatcctt acctccgatc gcaaacccag tgagattgac catcttgaag accggttggt
     9301 ttcccgattt atgggcggac ttactgttga catccaacta ccagactacg aaatgagagt
     9361 cgctattatc aaacaaaaaa ctgaagaatt tcaaattcaa attactcccg aagccatctc
     9421 ttttttggct gccaacatcg aatcaaacat tcgggaaatc gaaggtaaac ttcagcaaat
     9481 tttagttcaa tcttcctctc aaaaaattat tcccattgac cttacttttg ttcaaaactt
     9541 ctttaatccc gatcaaaatt taaatcaatt ttccactgcc aaaattaatc cccgccatat
     9601 tatttccgtc tgcgccaaaa cttttaacat caaaaccagc gacctttgtg gtaagagccg
     9661 aaaaaaagat ttggtttttg cccgacacct cgccgcttat attcttatta ccgaagccaa
     9721 tctcccttta gaagaagttg gtcgcctcct tggcggtcgg gatcacacca gtattatgca
     9781 tgcccgagac aaaatccacc aagatttatc cactaatcca caaatcagca aacttatcaa
     9841 ccaaattaaa tcatcttttt aaccttattt tacttatcca ccaaattgac tcacttatcc
     9901 acttactttt ccacatttat tttttaaatt ttccaccgaa actaacccca actcaattct
     9961 actttataag ccataaatct caaaaaaccc ttattttttc accttatatc tcatatcaac
    10021 aatcaaaaaa atattttata tcctatcctg tctcaaaaaa aaatattaat tcacttacca
    10081 accatcccta ctaactacaa ctactatttt gttattaatt ttatataata aaaacaatga
    10141 aactttctct tctccaggag aacttaaacc ttgctttaac caacgtttcc cgttttgttt
    10201 cttctaaaaa tcaattacct attttaggta atattttaat ctctaccgat caaggccgac
    10261 ttaaattatc tgccaccaat cttgaattaa gcattaatta ttggattggc gccaaaattg
    10321 atcaagaggg gagtattact ctaccttcaa aagaaattac cgaatttatt tcttatcttc
    10381 ccgccggcaa aattgattta tccctcaaag acaataattt attttctgtt tcttctacca
    10441 aaattgaatc tgattttact acttccccag ctaccgattt tcctgaattc cccaaaatta
    10501 accctgatac tgtttttgaa attgatcttg atattttaag tcaagctatc tctcaaatgg
    10561 ctttttcagc tgctaccgac gatactcgac cgattttaac cggtatctta tgttcttttt
    10621 cccaaaattc tctcaccctg gtttccactg acggttttag actttctttc aaagaaatca
    10681 aactggttaa ttctcttaat ttaaaaactg ataaatcggt cacattttta attcccgccc
    10741 gcagtttaat tgaagtcact aaactggcta aaacaaatca aaaagttaaa attggtctca
    10801 cactagacga acaccaagta atgtttgtct tagacgatat agagttggtt tcccgactta
    10861 tagaagggga gtttcccgac tacaaaaaac tcattcctga atcttatcag actaaaattt
    10921 ttattaatcg tgatgaactt tttcaatcag ttaaacttgc ttctgttttt gcccgggaat
    10981 ctgctaatgt cgtcaaaatg aatatcaaaa ataattctgt cgagcttacc gccaatgctc
    11041 ctcaggttgg tcaaaatcgg attgaaattg aaaccaaaac cgaaggagaa aaccttgaag
    11101 ttgctttcaa ttacaaattt ttaagtgatt ttcttaatat ttgcaaaagc c
//
LOCUS       DAOSHV010000008        10888 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_5075_length_10888_cov_6.237700, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000008 DAOSHV010000000
VERSION     DAOSHV010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 10888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10888
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5075_length_10888_cov_6.237700"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(93..974)
                     /locus_tag="PKZ29_00735"
     CDS             complement(93..974)
                     /locus_tag="PKZ29_00735"
                     /inference="COORDINATES: protein motif:HMM:NF020032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37430.1"
                     /translation="MNKIAVFGGNVAGSKFLELKVVADKLGVELDLISFKELAFETET
                     GKVFLRNHEIGDYDVYFFRNNKTYWEETSLILDQLGEDKIIIDPIIKRGRQSDVCKAH
                     QMVVLSQAGLPVPKTIYGSLKFLKKEDITKFEFPLIIKGSRGDRRRQVFKIYGQKDLK
                     EKLEEIKEIEKMGENKYMLQEYVVNSKDYRVMVVGGKVLGVMMRAIGDNPRLKDVFEK
                     SDLPEEIKVMAVRAAEVCGMAIAGVDVVFRDDEITKPLLFEVNKTPNYTRFEEVTGLK
                     VAEEVVKFLAEVGNEGI"
     gene            complement(992..2350)
                     /locus_tag="PKZ29_00740"
     CDS             complement(992..2350)
                     /locus_tag="PKZ29_00740"
                     /inference="COORDINATES: protein motif:HMM:NF025755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-transfer associated ATP-grasp
                     domain-containing protein"
                     /protein_id="HOG37431.1"
                     /translation="MFNLKKYHRFLTKNERNKVYLRKNTAVGKAIADSKYRTKKKLMT
                     AGVSVPELIARFKNQDDLNNFKWEKLEGNFVIKPESGYGGEGILIIRKRGKWAGEWLK
                     MNGEVLPTADLQRHCQEILAGKYSLHSMPDTVLVEERIKIHPLFLALTKSGTPDIRVI
                     VYNRIPVMAMFRIPTEKSGGKANLHQGAMGLGVDLATGITTFGIEGGGMTVSRIYDFN
                     KKKKRKVNGIKIPFWREILETAVKCQVALPELGYMGVDVVLDKEKGPEVLEVNARPGL
                     SIQLCNRAGLRARLEKVDEIEVRSVDHAITLAKYLFGEKFFEKVEEKEKNRLIEPVEN
                     IRVKIPKGFKPEFDKNRIIKKGKHRMIEVKAKVDTGALRSSIDHKLAKELGLLTEERI
                     LYYRHYRSALGSAHERPVVWLTFWLKGKKISTAVNVANREGLSSQFLIGRRDLTGFLI
                     KN"
     gene            complement(2375..3316)
                     /locus_tag="PKZ29_00745"
     CDS             complement(2375..3316)
                     /locus_tag="PKZ29_00745"
                     /inference="COORDINATES: protein motif:HMM:NF025760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM domain-containing protein"
                     /protein_id="HOG37432.1"
                     /translation="MKKITALLYIVLGWSCLTLAVKVEAKKPERLVTPTSIPTMVLVK
                     SLNLSPAPTETVATETAGVNTEITPTLAPVVNIKEEPKSTGLEYKSEKWNGLNTLKVA
                     IGWAVGKGISTNTLVLLLLLPLVATLVSFLHYVVGLSGYGIFMPTMVAVTFLATGIPG
                     GLVLFGVVLLLSLLSKSILRRLKLHFWPARSIGLIIISIGTFIFMVWSSAFSGIDISQ
                     VSIFPILFMILLAEEFTRTQLLKSKKEAKKLMLGTIVLAVAGATIMNWVWMQNLVLEY
                     PDLILVLILIANLAVGNYSGIRWSEIARFKKGIREKK"
     gene            3469..3807
                     /locus_tag="PKZ29_00750"
     CDS             3469..3807
                     /locus_tag="PKZ29_00750"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37433.1"
                     /translation="MKPSLLAQVDIGNNLLDNSGAAVGPKYTSISPLISSLLKNSLTI
                     AGIIFLGLLIFGGISLIINAGSSDSKKAAQAQQTVSSALIGFAVVFSAYFIIQIISLI
                     TGVKILDSGL"
     gene            3814..4134
                     /locus_tag="PKZ29_00755"
     CDS             3814..4134
                     /locus_tag="PKZ29_00755"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37434.1"
                     /translation="MLQIFGTITNPTGYDSQEGSGLFTLLTNIFRLAGVVAGIFFIVQ
                     LILAGYGYLSSSGDSKKTEAAWNKITQSLIGLGIVAAAFIIASVVGAILNIDILNPTI
                     HGPN"
     gene            4121..4471
                     /locus_tag="PKZ29_00760"
     CDS             4121..4471
                     /locus_tag="PKZ29_00760"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37435.1"
                     /translation="MVQIKPLIAVTYKLEGPGLAPDAVNPAKPLESLISQIIGILTIV
                     GVIYFIFQIILAGYSFISAQGDKNKIEAARSRLTNGILGLTIVVVALGIGAFIATLLG
                     IKDPLNIQNLVPKG"
     gene            4477..4830
                     /locus_tag="PKZ29_00765"
     CDS             4477..4830
                     /locus_tag="PKZ29_00765"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37436.1"
                     /translation="MSNLIKPIFAQINNPVLPNSGSIVSSPSTYVNSVIQSVISIFLI
                     VAVLYFVWHFIMYAYHMISSQGNPDKWKEAQNSILYAFIGLLLVFAIFAILKFVGIIF
                     GIPSLQSLQLTWPSL"
     gene            4876..5253
                     /locus_tag="PKZ29_00770"
     CDS             4876..5253
                     /locus_tag="PKZ29_00770"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37437.1"
                     /translation="MLNKLKQQIQYAAIAAPIYAQESIEIKSKTSDFQTLGNLTAGGI
                     ISGAVSLVLIVVALVFFFILVLGGLKWVTSGGDEKKVGEARAQITNALIGLAIVFAAW
                     AIMKLIGTVFGIDILKLSIPTFQ"
     gene            5320..5676
                     /locus_tag="PKZ29_00775"
     CDS             5320..5676
                     /locus_tag="PKZ29_00775"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37438.1"
                     /translation="MNLITQIFLPKPALAQTQVWSGRCVSDGVATIQGFECLFQNIIS
                     VVFSLAGLAFFAMFIVGGFKYLTSGGDSKKIAAASTTLTYAVIGVVGIIVSWLVLLFI
                     EQFTGIKVTIFKIPTT"
     gene            5680..7887
                     /locus_tag="PKZ29_00780"
     CDS             5680..7887
                     /locus_tag="PKZ29_00780"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HOG37439.1"
                     /translation="MIKKILLILSFFCLFWLNTPVVLAQPDPSPATQPQTQQNVFVVI
                     WEAIVRFFNYLIFGITYISEDYQASTKDTRQFTNDYTREDKDIASRAIPESVKKYRKG
                     VWFYEVLTKKNNKYQDELIKSDQLEVCPDIKITEIIAVMCQQGQNILYERDKPNQPLS
                     CQQAPIPSFTPKHPVSCYLNAYNNIQEVPQGLFMNEENAAPMTSTQLNDTIRNVMPIT
                     FQGETAPPTNDTPQQAKKLAEDSDKQERNMLLGILADKVHDQIACSDKADFNRDNLRD
                     AFACHMMPNSWNNPKCTALGQILDLDQIQCVEKDTVKNGPGLSQRGTHGLALSGYKYK
                     DIVNLYHGDGVDVQRLGNDLEGPIKVVLRTDYGKNSDTTCPNLVGKQAIFYDKIDLYR
                     GSGGVNEDGKTPCNKYVRIPVDDPNNPPSPPYELKGICYPVVHMDTLYNYLLGVAEVS
                     RNWHIEAQKALALTHRHTAVQNRGKGFLWDDAADQAFRCDQVSLNLPKIVDGKKVITN
                     QGAAVELTRGEFVVDKYSRRRLHSTARTAFCGPGSYNPKFDGLKYEKISYAYQGGNNA
                     INGICFEGVGYTMPNIDIALSPDGSYLQEYQYDPNSPVLGQTTDNQNNQSATCLLDNR
                     LFSPLNQLISAFNSQNPDNRLTFLSCYRSTAQQQQLWDQYLAENKGDKLKTISQINYP
                     GTSPHQTGRAIDFGDKSGKLTSQSPAYKWLVSNASRFGFYHHQLEPEHWNYKP"
     gene            7891..8355
                     /locus_tag="PKZ29_00785"
     CDS             7891..8355
                     /locus_tag="PKZ29_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37440.1"
                     /translation="MLKKITQNKYILILIAIVIILIVIKIISSTSVNNTPLPTNTKPG
                     QPSSPTPSSSNSVSSPENYTSGPSPTNSTSTSQSNSSSTNRITEINYQIPLYNLLPYQ
                     GKYFSARRYIGANNLEIIVPRQDQTDLAKQEAQAWFVQNGVDQLDRFTVIYK"
     gene            8400..10085
                     /locus_tag="PKZ29_00790"
     CDS             8400..10085
                     /locus_tag="PKZ29_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37441.1"
                     /translation="MTFRQLLHHLNLRNTRLLPSKKFFVITFLIILSGFSIFFSLKTI
                     TAQTEPNTTAAGNLIKQKQDAIEKGNNQEAWLDSSLSSNMMSLLISLGGKIPFDEDGN
                     LNLNTSYIPGGAIGTGTNMISSLYYQPVSGIEYLAQVKDNFLGKPAYAQNTGFQGLSP
                     LLPAWKAIRNIVYVFVSLVFITIGIMIMLRVKISPQATVTIQNSIPKIITTLVLVTFS
                     YAIAGFVIDLTKLILAFGLSLFFQIKGKGYGEDLLPTKFTEGGGIPIISWLGNLIKMG
                     YDTVAGWFVGPPNTHEKLSNMSFFSIYELTNRAIPAASGIALGEMFGQIMVGTILGGV
                     GSAVLGGLGRDLLVAGGTVIGDVVGGIGGIIIIPLIIAIFMVIFMIKLYFGLVKCYIT
                     LIFKIIIGPLQIGLGAFPSAKSGFSQWITEIAANAVVFPITSLFVVALNYLTDVLSGG
                     GLWIPSQISMNTISGNMAVVSAGVGLAGLAALSKLPKLVPEAVFKLKPDAFGTAIGDS
                     YKPLSKAGKVGFEMGRNKFVDSATSSAGTYDGRGGIIRQGLYYGARALGRLKH"
     gene            complement(10093..>10888)
                     /locus_tag="PKZ29_00795"
     CDS             complement(10093..>10888)
                     /locus_tag="PKZ29_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37442.1"
                     /translation="VQYHLELDKIRGTSSFRDKLRNREIRPLPERMGCSLVEMLYVNY
                     EENGSLKMEEKKKKNGEVELDDNGNPKMVESKMLMSKALSLGKKISFPKDIEGEDLDI
                     FTDFRDAMDGTKTAFDYLNGKNRLELAKNEHEWARNFQKDIALIRQTKTESTSSGRKY
                     LSFLDDPEFMVWVIAASTGFESLADDIMLDAKSIKYDGSNYKGRIREIVNLPGLVPIR
                     DRQAVLERLGGDKGEMLTMMRLAKITQQVVKNRRDLMAGLEREKRK"
BASE COUNT         2938 a         2814 c         1611 g         3525 t
ORIGIN      
        1 atctccgacc cccgtcttat caggacggtg ctctaaccaa ctgagctaat cacccaaaaa
       61 gtaatcctat tctaccaaca tctcaaccaa aattaaatac cttcgtttcc aacctctgcc
      121 aaaaacttta ccacttcctc tgccactttt aacccggtta cttcttcaaa tctagtgtaa
      181 tttggggttt tatttacttc aaacaacagt ggtttggtta tttcatcatc tctaaatacc
      241 acatccaccc ctgctattgc cattccacac acctctgccg ccctaactgc catcactttt
      301 atctcctctg gcaaatcact tttttcaaat acatccttaa gtctcggatt atccccgata
      361 gctctcatca ttacccccaa cacctttcct cccaccacca tcacccgata atccttgctg
      421 ttgacaacat attcctgcag catatattta ttctcaccca ttttttctat ttcttttatc
      481 tcctccagct tttctttcag atctttttga ccataaattt taaacacctg ccttcttcta
      541 tccccccggc tacctttaat aattaaaggg aactcaaatt tagttatatc ttcctttttc
      601 aaaaatttta aactcccata aatagttttt ggcaccggca gccccgcctg actcaaaacc
      661 accatttggt gagccttaca cacgtcactc tgccgtcctc tttttataat cggatcaata
      721 ataattttat cctcacccaa ctgatccaaa atcaaacttg tttcctccca ataagtcttg
      781 ttgttccgaa aaaaataaac atcataatcc ccgatttcat gatttctcaa aaatactttt
      841 cccgtctccg tctcaaaagc caattcttta aatgaaatca aatccaattc cacccccaat
      901 ttatctgcca ccaccttaag ctccaaaaat ttactccccg ccacattgcc tccaaacact
      961 gcaattttat tcatcttttt cattttttaa tctaattttt aatcaaaaat cccgtcaaat
     1021 ctcttctccc aatcaaaaac tgggaactta acccttcccg attagctaca tttaccgctg
     1081 tcgaaatttt cttgcccttc agccaaaatg tcagccatac caccggtctt tcgtgagcgc
     1141 tgcccaaagc cgaacggtaa tgacgataat acaaaattct ctcctcagtc agcagcccca
     1201 attccttggc taatttatga tctatcgaac ttctcaatgc ccccgtgtct accttggcct
     1261 tcacctcaat catccgatgt ttccctttct ttataatcct atttttatca aactcgggtt
     1321 taaatccttt aggaattttt accctaatat tttccaccgg ctcaatcaat cggttttttt
     1381 ctttttcctc taccttctca aaaaatttct ccccaaacaa atatttagcc aaagttattg
     1441 catgatcgac actcctaacc tcaatttcat ccaccttttc caatctggcc ctcaaccctg
     1501 cccggttaca taattgaatt gacagacctg gtctggcatt tacctccaac acttccggcc
     1561 ccttctcctt atccaacact acatccaccc ccatataacc taactccggc agcgccacct
     1621 ggcactttac cgccgtctcc aaaatctccc gccaaaatgg aatttttatc ccattaactt
     1681 ttctcttttt ctttttatta aaatcataaa ttcttgacac cgtcattccc ccgccttcaa
     1741 tcccaaacgt agttataccc gtggctaaat ctactcccaa ccccattgct ccctgatgca
     1801 aattagcctt accacccgat ttctctgttg ggattctaaa catggccatc accggtatcc
     1861 ggttgtaaac aatcaccctg atatccggcg tacctgattt agttaatgcc aaaaacaatg
     1921 ggtgaatctt aatccgctct tccaccaaca ccgtatccgg catactgtgt aaactgtatt
     1981 ttcccgccaa tatttcctga caatgtctct gcaaatccgc cgttggcaac acttccccat
     2041 tcatttttaa ccactcaccc gcccattttc ccctcttcct gataatcaaa attccctctc
     2101 ccccatagcc cgactccggc ttaattacaa aatttccctc cagtttttcc cacttaaaat
     2161 tattcaaatc atcttgattt ttaaatctgg caatcaactc cggcaccgac acccccgccg
     2221 tcattaactt ttttttagtc cgatatttac tgtcggcaat cgccttgccc accgccgtgt
     2281 ttttgcgcaa atatacctta tttctttcat tctttgtcaa aaatcggtga tattttttta
     2341 gattaaacat aggttttaat tatcaaaccg ccaactattt tttctctcta atcccttttt
     2401 tgaatctagc aatctccgac caccgaattc cgctataatt tcccaccgcc aagttggcta
     2461 tcagtattaa aactaatatt aaatccggat attctaatac caagttttgc atccataccc
     2521 agttcattat tgtcgctcct gccactgcca acactatcgt tcccaacatt agtttttttg
     2581 cctctttttt gctctttaat aactgtgttc tggtaaattc ctctgccaac aaaatcatga
     2641 ataaaatcgg aaaaatcgat acttggctta tatcaatccc gctaaatgcc gatgaccaca
     2701 ccataaatat aaatgtccca atagaaataa ttatcaatcc aattgatctc gccggccaaa
     2761 aatggagctt cagtcgtctc aaaattgact tgctcaaaag cgacagcagc aacaccaccc
     2821 caaacaatac caaccctccc ggtattcccg ttgccaaaaa agttaccgcc accattgtcg
     2881 gcataaaaat cccatatccc gacaaaccca ccacgtagtg caaaaatgat actaatgtcg
     2941 ccaccaacgg cagtaataac aacaagacca gtgtattagt tgatatcccc ttacctaccg
     3001 cccaaccaat cgccactttt aatgtattta aaccattcca cttctccgat ttatattcca
     3061 accccgtgct ttttggttct tcctttatat ttaccaccgg cgccaaagtc ggtgttattt
     3121 ccgtattcac ccccgctgtc tccgtagcaa ccgtttcagt cggtgctgga cttaaattta
     3181 aacttttcac caataccatt gtcggaatcg acgttggagt caccagtctc tccggctttt
     3241 ttgcttcaac tttgactgct aatgtcaaac aactccaacc taatactata tatagtaggg
     3301 cagttatttt tttcatactc taattttact ttaaaaatta actgaaatct gatatctgat
     3361 aaactgaata acttataaca aagtgccgta ttatagcatt gttttaacca aatctatttc
     3421 catcttcacc aattcatttt ttttcctctt taaggtataa ttttgccaat gaaaccatct
     3481 cttctggctc aagttgatat cggcaacaat ctccttgata acagtggcgc tgctgtcggt
     3541 cccaaatata ccagtatcag tccccttatt agttctcttc tcaaaaattc ccttactata
     3601 gccggaatta ttttcctcgg gcttcttatt tttggtggta ttagtctcat cattaatgcc
     3661 ggttcttccg attccaaaaa agctgcccaa gcccaacaaa ctgtcagcag tgcccttatc
     3721 ggctttgctg ttgttttttc cgcttatttt ataattcaaa ttattagtct tattaccggc
     3781 gtcaaaattc ttgattctgg tttataataa cttatgctcc aaatctttgg aactattacc
     3841 aatcctaccg gctacgacag tcaagaaggt tccggtttat tcactctttt gacaaatatt
     3901 ttcagattag ctggagttgt tgccggtatt ttctttattg ttcaacttat tttagctggc
     3961 tacggctacc tttccagcag tggtgattcc aaaaaaactg aagctgcttg gaataaaatt
     4021 acccaaagtc tcattggtct gggcattgtc gctgccgctt ttattattgc cagcgtcgtc
     4081 ggcgccatat taaatattga cattctcaac cccaccatcc atggtccaaa ttaaacccct
     4141 aatcgctgtc acttataaac tagaaggtcc cggacttgcc cccgatgctg tcaatcccgc
     4201 caaacccctt gaaagcctta ttagccaaat aattggtatt ctcaccattg tcggcgttat
     4261 ttattttatt tttcaaatta ttcttgctgg ttattccttt atctctgctc aaggcgacaa
     4321 aaataaaatt gaagctgctc gcagtcgact taccaacggt attcttggtc tgactatcgt
     4381 cgttgttgct ctcggtattg gcgcttttat cgctactctc ttaggtatca aagatccgct
     4441 taatattcaa aaccttgttc ccaaaggcta atttttatgt ccaatctcat caaacctatt
     4501 tttgctcaaa tcaacaaccc tgttttaccc aattccggca gtattgtttc cagtccctca
     4561 acctacgtca atagtgttat tcaaagtgtt atttctattt tccttatcgt cgctgtcctc
     4621 tattttgttt ggcattttat tatgtatgcc taccatatga tttcttccca aggcaaccct
     4681 gacaaatgga aggaagctca aaattcaatt ctttatgcct ttatcggtct tcttttagtc
     4741 tttgctatat ttgccattct taagtttgtc ggcattattt ttggcattcc cagtctccaa
     4801 agtctccaat taacctggcc ttccctttaa ttttttcaaa aacctgttat tatttaatta
     4861 attattaata aaattatgct aaataaactc aaacaacaaa tccaatacgc agctatcgct
     4921 gctcctatct atgcccaaga gagtatagaa ataaaatcta aaactagcga tttccaaaca
     4981 ctaggcaacc ttactgctgg tggtatcatt tccggtgccg tcagtttggt tttaattgta
     5041 gttgctttag ttttcttttt cattctcgtc ttaggtggtc ttaagtgggt cacttctggc
     5101 ggcgatgaga aaaaggtcgg tgaagctcga gctcaaatca ccaacgccct catcggttta
     5161 gccattgtct ttgccgcttg ggctattatg aaactcattg gcaccgtctt tggtattgac
     5221 attcttaagc taagtattcc tactttccag taaatttatt tggctttcaa aacccgcctt
     5281 tctggcgggt tttttgttgg tctattgtat aataaattta tgaacttaat cacccaaatt
     5341 tttctgccca aacccgcttt agcccaaacc caagtatggt ctggtcgttg tgtctctgac
     5401 ggtgttgcta ctattcaagg gtttgagtgt ttgtttcaaa atattatctc ggtagttttc
     5461 agcctggctg gcttagcttt cttcgctatg tttattgttg gcggttttaa atatctcacc
     5521 tctggcggcg actccaaaaa aattgctgcc gcctccacca ctcttactta tgccgttatc
     5581 ggcgttgtcg gcatcattgt ctcctggtta gttctcctgt tcattgaaca atttaccggt
     5641 atcaaagtca ctatattcaa aatcccaaca acctaaataa tgatcaaaaa aatcctgctc
     5701 attttaagtt ttttttgcct attttggctt aatactcctg ttgttttagc tcaacctgac
     5761 ccttctcccg ccactcaacc tcaaactcaa caaaacgttt ttgttgttat ttgggaagct
     5821 atcgttagat tttttaacta ccttatcttt ggtatcacct acatttccga agattatcaa
     5881 gcctctacca aagacacccg ccaatttacc aacgactata cccgcgaaga taaagatatt
     5941 gcctcccgag ctattcccga gtctgtcaaa aaataccgta aaggtgtttg gttttatgaa
     6001 gtcctcacca aaaaaaataa taaataccaa gatgaactta tcaaaagtga ccagctagaa
     6061 gtttgtcctg acatcaaaat tactgagatc atcgccgtta tgtgtcaaca gggccaaaat
     6121 attctttacg aaagagataa accaaaccaa cctctaagtt gtcaacaagc ccccattcct
     6181 tcttttacgc ccaaacatcc tgtttcttgc tatcttaatg cctacaataa tattcaggaa
     6241 gttcctcagg gattatttat gaatgaagaa aatgccgctc ccatgaccag cacccaatta
     6301 aatgacacca tccgcaatgt tatgcctatc actttccagg gtgaaaccgc cccgcccacc
     6361 aacgataccc cccaacaggc caaaaaactt gccgaagata gcgacaaaca ggaacgcaac
     6421 atgcttcttg gtattcttgc tgacaaagtt catgaccaaa ttgcctgcag cgacaaagcc
     6481 gactttaacc gggacaatct ccgtgacgct tttgcttgtc acatgatgcc caatagctgg
     6541 aacaatccaa aatgtaccgc tctcggccaa atccttgatc ttgatcaaat ccaatgtgtt
     6601 gaaaaagaca ctgtcaaaaa cggtcccggc ctcagtcagc gcggcaccca cggtctggct
     6661 ctcagtggtt ataaatataa agatattgtc aacctttatc atggcgacgg agttgatgtc
     6721 cagcgtctag gtaatgatct tgaaggaccc atcaaagttg ttcttaggac agattacggt
     6781 aaaaacagtg acactacttg tccaaatctt gttggtaaac aagccatttt ctatgacaaa
     6841 attgatcttt accgcggtag tggtggagtc aatgaggatg gcaaaactcc ctgcaataaa
     6901 tatgtccgaa ttccggttga tgatcctaac aaccctccca gtccacccta tgaacttaaa
     6961 ggtatttgtt acccggtagt tcatatggat actctctaca actatcttct cggagtcgcc
     7021 gaagttagcc gcaactggca tattgaagcc caaaaagctc tggccctgac ccatcgtcat
     7081 accgccgtcc aaaaccgcgg taaaggtttc ctttgggacg atgctgccga ccaggccttt
     7141 cgttgcgacc aggtctctct taatttaccc aaaatcgtcg acggtaaaaa agttatcacc
     7201 aatcagggcg ccgccgttga actcacccgt ggcgaatttg tggttgataa atacagtcgc
     7261 cgccgtcttc actccactgc ccgcaccgcc ttctgtggac ccggttcata caatcccaaa
     7321 ttcgatggtc taaaatacga aaaaatttcc tacgcctatc aaggcggtaa taatgccatc
     7381 aacggtattt gttttgaagg tgtcggttac actatgccaa acatagatat tgccctctca
     7441 cccgacggtt cttaccttca ggaatatcaa tatgatccaa attcaccggt cttagggcag
     7501 accactgata atcaaaataa ccaatccgct acctgccttc tcgacaatcg tcttttttct
     7561 ccattaaacc agcttatttc tgccttcaac tcccaaaatc ccgacaatcg ccttaccttt
     7621 cttagttgtt accgcagcac cgcccaacag cagcagcttt gggatcaata tttagccgaa
     7681 aacaagggcg acaaactcaa aaccatctcc caaattaatt atcctggcac ttctccccat
     7741 caaaccggtc gggctattga ttttggtgac aaatcaggca aattaacctc acagtctcct
     7801 gcctataaat ggctggtttc caatgcctcc cggtttggct tttatcatca ccagttagaa
     7861 ccggaacact ggaactacaa accctaaatt atgctcaaaa aaataactca aaataaatat
     7921 attcttatct tgatagctat cgttattatt cttattgtca tcaaaattat ttcctccact
     7981 tctgtcaaca atacccctct tccgactaat accaagcccg gacagccgtc ttcccccacc
     8041 ccatccagct caaactctgt ttcttcacca gaaaattata cctccggccc atctccaacc
     8101 aattctacct ccacttcaca gtcaaactct tcatccacca accgaattac cgaaatcaac
     8161 tatcaaattc ctctttataa tctcctgcct tatcagggca agtatttctc cgcccgccgt
     8221 tatattggcg ccaacaacct ggaaatcatt gtcccccgtc aagaccaaac cgacctggcc
     8281 aaacaggaag cccaagcttg gtttgtccaa aacggtgttg accaacttga ccgttttact
     8341 gttatttaca aataattacc actttattta ttaagtttgc taaaattaat tattaaccta
     8401 tgaccttcag acaattgctt catcatctca atctccgcaa cactcgttta cttccttcca
     8461 aaaaattttt tgttatcacc tttctcataa ttttgtctgg tttttctatc tttttttctc
     8521 taaaaactat tactgctcaa actgagccta ataccaccgc tgccggaaac ttaattaaac
     8581 aaaaacaaga tgctattgaa aaaggcaata atcaggaagc ttggctcgac tcctctctta
     8641 gtagcaacat gatgagtctc ttaatttctc ttggtggcaa aattcctttt gatgaagatg
     8701 gtaacctcaa tctcaacacc agttatattc cgggcggcgc cattggtacc ggtactaata
     8761 tgattagtag cttatactat caaccggttt ctggaattga gtatttagcc caggtaaaag
     8821 acaactttct gggcaaacca gcttatgccc aaaatactgg ctttcaggga cttagccccc
     8881 tccttcctgc ttggaaagcc attagaaaca ttgtttatgt ttttgtatcg ttagttttta
     8941 tcaccattgg tatcatgatt atgcttcggg tcaaaatcag ccctcaagct accgttacta
     9001 tccaaaatag tattcccaaa attattacca ctctagtcct ggttactttc tcttacgcca
     9061 ttgctggttt tgttattgat ttgaccaaat taattctggc tttcggactt tcattgttct
     9121 tccaaataaa aggaaaaggg tatggtgaag atttgttacc aaccaaattt acagaaggcg
     9181 gaggtattcc tattatcagc tggttgggta atcttattaa aatgggctat gataccgttg
     9241 ccggttggtt tgtcggccca cccaatactc atgaaaaact atcaaacatg agctttttta
     9301 gtatttatga actaaccaac cgagctattc ctgccgcttc cggcattgct cttggtgaaa
     9361 tgtttggtca aattatggtt gggacgatct taggtggagt tgggtctgcg gttctaggag
     9421 gactaggtcg tgatttatta gttgccggtg gaaccgttat tggtgatgtt gttggtggaa
     9481 ttggtggaat aatcattatt cccctgatta ttgctatatt tatggttata ttcatgatca
     9541 aactttattt tggtcttgtt aagtgttaca ttacccttat ttttaaaata atcatcgggc
     9601 cgcttcaaat cggtcttggc gccttcccgt cagccaaatc gggtttttct caatggatta
     9661 ctgagattgc tgccaatgct gttgtttttc ccatcaccag tttatttgtc gttgccctaa
     9721 attatctaac tgatgttctt tccggaggcg gtttatggat tccgtcacaa atctctatga
     9781 atactatttc aggtaatatg gctgtagttt ctgctggtgt tggtctagct ggtctggccg
     9841 ctttatctaa acttcccaag ctcgtccctg aagccgtctt caaactcaaa cccgatgcct
     9901 ttggtacagc tattggtgat tcatataagc ctctttcaaa agctggtaag gttgggtttg
     9961 aaatgggtag aaataagttt gttgattcag ccacttcttc ggccggtaca tatgacggac
    10021 gtgggggaat aataagacaa gggctttact acggagctag agccctagga agattaaaac
    10081 attaatattc agttattttc gcttttctct ttccaatccc gccattaaat ctcttctgtt
    10141 tttaacaact tgttgagtta ttttagccaa tctcatcata gtaagcatct cacccttgtc
    10201 tccaccaagc ctctctaaaa cagcctgtct atctctaata ggaaccaatc caggcaaatt
    10261 tactatctcc ctgattcttc ctttatagtt agatccgtcg tatttaatcg atttagcatc
    10321 caacataatg tcatcagcca aactctcaaa gcccgttgaa gccgctatca cccaaaccat
    10381 aaactccgga tcgtccaaga atgataaata cttccttcca ctactagtgg attcggtttt
    10441 agtttgtcta atcaatgcaa tatctttttg aaaattccta gcccactcat gctcattttt
    10501 agccaactct aacctatttt tgccatttaa ataatcaaat gcggttttag ttccatccat
    10561 cgcatctcta aaatcagtaa agatatctaa atcctctcct tcaatatctt ttggaaaaga
    10621 aatttttttc cccaacgaca aagccttact cattaacatt tttgattcaa ccattttagg
    10681 gtttccatta tcatcgagct ccacctctcc attctttttt ttcttttcct ccatttttaa
    10741 actaccattc tcttcgtaat tcacatacaa catttctacc aaactacaac ccattctttc
    10801 tggtaatggt cttatttctc tattgcgcaa tttgtctcta aaactgcttg ttcccctaat
    10861 tttatctaac tctaaatgat attgaacc
//
LOCUS       DAOSHV010000009        10100 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_5672_length_10100_cov_5.400498, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000009 DAOSHV010000000
VERSION     DAOSHV010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 10100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10100
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5672_length_10100_cov_5.400498"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(245..1513)
                     /gene="eno"
                     /locus_tag="PKZ29_00800"
     CDS             complement(245..1513)
                     /gene="eno"
                     /locus_tag="PKZ29_00800"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010244980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOG37443.1"
                     /translation="MSKIKNIFAREILASGGAPSLEVTVTLESGAVGEASVSYGASAG
                     SKEATVLLDGDKARYNGKGMLTAINNINTKIKEVLVGMEAANQREIDQKMIDLDGTVN
                     KAVLGGNAMLGVSLAVCRAEAEEEKMPLYKYLRKTYGIGDIGWTLPKPMIVMIEGGKH
                     ADETTDLQEFMVGAMGDKTAAENVRMEMEIYEALKKILKAEKLSTNVGNEGAFAPNGI
                     SSNEKPMEYLVEAIKSAGYTPGVDAGVGIDAAASEFYSEETKKYSLKLEGKELSSEEL
                     IAYYKPWIEKYPMATMEDMLSEFDWDNWPKMVEAIGGKFPLIADDLTVTNTNLWKKAI
                     EMKAATAILIKLNQAGTLTETVDCCLLAREHNLMTMPSHRGGGETNDTFLVDLAVAVG
                     SAYIKVGPTRGERVCKYNRLMRIEEELKSL"
     gene            complement(1545..>1801)
                     /locus_tag="PKZ29_00805"
     CDS             complement(1545..>1801)
                     /locus_tag="PKZ29_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37444.1"
                     /translation="REAGMSGKRMMEFLRKVYRKIWWIDEWLYIYKNLDQKEIDKICA
                     TNKTGYADLWMKARNTSKDLINSKIDFKLKQAIKKRWFKL"
     assembly_gap    1802..1811
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1812..2382)
                     /locus_tag="PKZ29_00810"
     CDS             complement(<1812..2382)
                     /locus_tag="PKZ29_00810"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HOG37445.1"
                     /translation="MKLRRAKIGEKINFFYLENDRYIGQRAAVGKYEPYETQLILRQA
                     KVGGTVIDVGANIGYYTLILADKVGKNGKVYAFEPDKINFEILEKNIKENKLKNVVAI
                     KAAIGSKNESKILYKSEENLGDHKLYKPKTPAKKREKVKIVRLDSFIKEKMDLIKIDT
                     QGWEPEVIEGAKEMIKKNKPVIFLEYSPAS"
     gene            2483..2773
                     /locus_tag="PKZ29_00815"
     CDS             2483..2773
                     /locus_tag="PKZ29_00815"
                     /inference="COORDINATES: protein motif:HMM:NF012393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOG37446.1"
                     /translation="MSHTIHPVPGYLLIEPQKQEKTTASGIVLPDNHQDKPQQGKVLA
                     VGGSIIDDGHEIKPPCNIDDIVIYREWGGKEYKLDDQELLLLKFDDIMAVIK"
     gene            2815..4476
                     /gene="groL"
                     /locus_tag="PKZ29_00820"
     CDS             2815..4476
                     /gene="groL"
                     /locus_tag="PKZ29_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009711075.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOG37447.1"
                     /translation="MAKQLKFGQDARQSLLNGINTLADAVITTLGPKGRNVAIDKKWG
                     GPTVIHDGVSVAKEIELENPYENMGAQLIKEAASKTNDIAGDGTTTATVLAQAIANKG
                     LQNVAAGANPMIIRRGLEKGLAAVIKELDGMKKDIKADDKDSIEQVATISAGDAEIGK
                     RIAEAVVKVGRDGLITAEEGKGLFLETKETSGMEFDQGFLSPYFATNTETMEAIIDNP
                     YIVITDKKISSIQEILPFLEKLVKLTKNFVIIADDIDGEALATLVVNKIRGTFNVLAV
                     KAPGFGDRRKAMLEDIAVLTNGQVISEDLGIKFDTAEPSDYCGRADSVTADKDKTRIL
                     GGAGAEVDIKNRVSQLRAQMSKTDSDYDQEKFQERISKLVGGAIVLQVGGATEVEMKE
                     RLERVKDAIDATKAAVEEGILPGGGVALLKASLKLDEVKANNDEEKVGLDILKYALEQ
                     PIRRLAQNSGEDAGYIFNLIKDNVAKDPKSDYGYNAATGKMESMVKAGILDPAKVTKS
                     ALTNAVSVSTMILTTDVLITDAPEKSSPASPAAGMGGMGGMDGMM"
     gene            4549..4619
                     /locus_tag="PKZ29_00825"
     tRNA            4549..4619
                     /locus_tag="PKZ29_00825"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4581..4583,aa:Cys,seq:gca)
     gene            4817..5047
                     /locus_tag="PKZ29_00830"
     CDS             4817..5047
                     /locus_tag="PKZ29_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37448.1"
                     /translation="MSTCAIIPEHDLTSYQEFGGLETGIATFTKWVDIRDQMTRPLRR
                     QTLNQDLLKFSDQLVLDGADRKDLNRIAGWTD"
     gene            5158..5844
                     /locus_tag="PKZ29_00835"
     CDS             5158..5844
                     /locus_tag="PKZ29_00835"
                     /inference="COORDINATES: protein motif:HMM:NF012487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37449.1"
                     /translation="MTRQRAPLTCFVGPMNSSKTRRLLDHLSECRDALNYSIRVYKPA
                     LDNRFDLNEIRCRNGGCWTAIPVPSSKPSRADSLFIFKDIYKLASRRRPNLVAIDEIQ
                     FFDKNIKEVVKCLLYDNIEVVVSGLNRSFRGEPFPAIESLLALASKVETLTARCTYKD
                     GHGKTCGAPATMTQRLINRQPAPYCSPTVVIQQIGDSSVTYEARCADHWFCPGVPISK
                     LKKTSKSSRG"
     gene            5837..6694
                     /gene="ispH"
                     /locus_tag="PKZ29_00840"
     CDS             5837..6694
                     /gene="ispH"
                     /locus_tag="PKZ29_00840"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HOG37450.1"
                     /translation="MANIIVAQPHGFCFGINRAIALARQTAQKFHQPIFFLGELVHNQ
                     HVVSWLEHDLNIKTVTDINQISPNSIVIIRAHGAAPQVFRRLRSRGLTLVDASCPLVL
                     KSHQTVRRLASAKKSIIFLCNKIDHDETVGVVGEAPKFVTPVTLDNIYQHSISHPDQT
                     VVITQTTLSTTETKAALDYLKNKYPGITILPHICQATTNRQQSIIKLTKKYHFVIIVG
                     SPTSANSNSLRQVAESAGATAYIVDNPTELKPEWFQNIDNIVVSSGASTPEDILLSVI
                     DQIKDFTRP"
     gene            complement(6681..7133)
                     /locus_tag="PKZ29_00845"
     CDS             complement(6681..7133)
                     /locus_tag="PKZ29_00845"
                     /inference="COORDINATES: protein motif:HMM:NF014009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOG37451.1"
                     /translation="MIKHKIVKFETKEYLEFRDITEEIEKFLKDSDIKEGQILVFSKH
                     TTVAICINEKEKGIVEDFRNLLKTILPKDGYYCHNDLKIRTENLVCQSGESDCLNGHS
                     HCTQLMMRNSEAIPVIGGKMTLGVWQRIFAIELDCCREREIVVQIMGE"
     gene            complement(7172..7510)
                     /locus_tag="PKZ29_00850"
     CDS             complement(7172..7510)
                     /locus_tag="PKZ29_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37452.1"
                     /translation="MIIKRIKKILKLTILIAARQIWKLLCNLYNLIREPVVVIKSLIK
                     SRDKSQIFLVVMVLLMPIISYFLARIVWDYYRYGVVIKDVGWWFGAAVAVQIGLLLYI
                     GYWVLKAVRK"
     gene            complement(7491..7991)
                     /locus_tag="PKZ29_00855"
     CDS             complement(7491..7991)
                     /locus_tag="PKZ29_00855"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOG37453.1"
                     /translation="MEDCYKKYSLGVKNFIRQKIDDEGVVEEITNDVMLAAMSSWKNF
                     RGQCSEFSWVCSIANHKIIDYYRKKKIKTILFSVSPVFEEIADKALTPERDALKNELK
                     KEIKKTLSDLKTGYKNLLRLKYIEEKKVAEIAKETNLTIKAVESRLIRAKKQFREAWV
                     YDNKKD"
     gene            8080..8874
                     /locus_tag="PKZ29_00860"
     CDS             8080..8874
                     /locus_tag="PKZ29_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016208995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOG37454.1"
                     /translation="MLTPNLPYSHLSRDLRLQLLDMIYESGSGHVGGCLSSLDILISL
                     YFSDLFNFNRDHFILSAGHLACALYTVLSAKNYFPSRLLSTYTDFNSLLQGHVSTDVP
                     GVEYSSGSLGQGLSFAAGLALGDKSHTTVCLTTDGEHQEGQIWESLMFAQKYQLSNLI
                     NIVDCNGTQVDGRIEEIMPLNNLAAKYVQFGWTVTTVNGHNLHQIAKALKSALNHSHS
                     GYPSCVIAKTVLGRGVSFMEHQFKYHDIKNLDPSLYLQAKNDLESY"
     gene            8861..9808
                     /locus_tag="PKZ29_00865"
     CDS             8861..9808
                     /locus_tag="PKZ29_00865"
                     /inference="COORDINATES: protein motif:HMM:NF014798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOG37455.1"
                     /translation="MNPTKTASIRQAFGDTLTQLAKSNRHLYVVSMDLKSSLHLTNFA
                     KKYPRRFIECGIAEANAAAIAAGLAKSGKTVFLCSFACFSPAINWAVIRQSICYNHLD
                     VKIVGSHAGLTTGELGATHQMLEDVALMTTLPNMEVFAPVDAIETQKITTTLSRSRRP
                     AYLRLVRPATSLLFDPKNTFTIGKSHLLKSGRDITVIGYGPILEQAFMAQSQLSLQKP
                     NISLEIINCSSIKPFDFTIISRSLKKTGRLICLEDHQQNGGLGHLVAAALLHHRLSPK
                     FIHLAVNNQFGRSAKNYSQLYDYYGLGVKNLITAAKKLL"
     gene            9805..>10100
                     /locus_tag="PKZ29_00870"
     CDS             9805..>10100
                     /locus_tag="PKZ29_00870"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF013296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HOG37456.1"
                     /translation="MKSLSQILTSARQHQTAIPAFNIDSFEIYQAVESVVADINQPCI
                     VQLSSGEDSFIQAERLFILVQKARLDGLPIYLNMDHGKDSHRLLHLLRLGFDMV"
BASE COUNT         2571 a         2614 c         1660 g         3245 t
ORIGIN      
        1 cccaaagtca ggtgaccgac ttcactcccg ccctgagccg attgaggatt gcccacatct
       61 tccccggtgc atttcaatat cgcgctgggg tattctgctg catatttatc cgcatttggc
      121 gtctttgcct ccatgatcaa attgccatac gggttctccg accaccccca tccatccctt
      181 attattagta ttactttttt atgtttcact aatctaactt gataaacttt ataactttca
      241 aactttataa acttttgagt tcttcttcaa ttctcatcaa tcgattatat ttgcagactc
      301 tttctcctcg ggttggtcct acctttatat aagctgaccc caccgctact gccagatcca
      361 ccaaaaaagt atcattagtt tctccgccgc cgcgatgact tggcatcgtc atcaaattat
      421 gttcccgcgc cagcaaacag cagtccactg tttccgtcag tgttcccgcc tgatttaatt
      481 taatcaaaat tgctgtcgcc gccttcatct caattgcttt tttccacaaa tttgtattag
      541 ttaccgtcag gtcatccgca atcaacggga atttaccccc gatcgcttct accatttttg
      601 gccaattatc ccaatcaaat tccgacaaca tatcttccat cgtcgccatc ggatattttt
      661 ctatccatgg tttatagtag gcgattaatt cttctgaact caactctttg ccttccagtt
      721 ttaaactata ttttttggtt tcttcactat aaaattcact ggccgctgca tcaattccta
      781 ccccagcatc aactccggga gtgtaaccag ccgattttat tgcttccact aaatattcca
      841 tcggtttttc gtttgatgaa attccgttag gggcaaacgc gccttcatta cccacattcg
      901 tcgataattt ttccgccttc aaaatctttt ttaaagcctc ataaatttcc atttccattc
      961 ttacgttttc cgccgccgtt ttgtctccca ttgcccccac cataaattcc tgcaaatcag
     1021 tcgtttcatc cgcatgtttg ccgccttcaa tcataactat catcggtttt ggtaatgtcc
     1081 aaccgatatc tccaattcca taagtttttc gtaaatattt atacaacggc attttttcct
     1141 cttcagcctc cgctcggcaa accgccaaac ttactcccaa catcgcattg ccgcccaaca
     1201 ctgctttatt caccgttcca tcgagatcta tcattttctg atcaatttct cgctggttag
     1261 cagcttccat tcccaccaac acctctttaa tttttgtatt tatattattt atcgccgtca
     1321 acattccttt gccgttgtat ctggctttat cgccatccaa caacaccgta gcttcttttg
     1381 atcccgccga cgctccataa ctgactgatg cctcacccac cgctccgctt tctaaggtca
     1441 ccgtcacctc taaacttggc gctccgcctg atgctaatat ttcccgggca aaaatatttt
     1501 ttatttttga catataaaaa aactaaattt taatttactc cccattataa cttaaaccac
     1561 cgctttttaa tcgcttgttt taatttaaaa tctattttgc tgttaatcaa atctttactg
     1621 gtatttcttg ctttcatcca caagtcggca taaccggttt tgttggtggc acaaatttta
     1681 tcaatttctt tttggtctaa gtttttgtaa atatacaacc attcatcaat ccaccaaatt
     1741 tttctataaa cttttctcaa aaattccatc atcctttttc cactcattcc tgcttctctg
     1801 tnnnnnnnnn nagctggcgg gggagtattc caaaaatatc actggtttgt tttttttaat
     1861 catctctttt gctccctcaa tcacttccgg ttcccaccct tgagtgtcaa tttttatcaa
     1921 atccattttt tcttttataa aactatccaa tcttactatc tttacttttt ctcttttctt
     1981 ggccggagtc ttaggtttat acagcttatg atcacccaaa ttttcttccg atttatacaa
     2041 aattttgctt tcatttttac ttcctatcgc cgcctttatt gcgactacat tttttagctt
     2101 attttctttt atattctttt ccaaaatttc gaaattaatt ttatccggtt caaacgcata
     2161 aacctttcca tttttcccca ccttgtcagc caaaatcaag gtgtaataac caatatttgc
     2221 tcccacatct atcactgtcc ctccaacttt tgcttgtctc aaaatcaact gagtttcgta
     2281 gggttcatat tttcccaccg cggctctctg accaatgtat cggtcatttt ccagataaaa
     2341 aaaattaatt ttttccccaa tttttgcccg ccttaatttc acatctcatt ttatcagaca
     2401 aacccttgac caaaccattt tcttggctat aatattagca gtcgattaac aagactgata
     2461 attattaatt caccaaaatt ttatgtctca cactattcat cccgtccctg gttacctttt
     2521 aattgagccc caaaagcagg aaaaaactac cgcttccggc attgtcctcc ccgacaatca
     2581 tcaggacaaa ccccaacagg gcaaagtttt agccgttggc ggatccatta ttgatgatgg
     2641 tcacgaaatt aaacctcctt gcaatattga cgatattgtc atttaccgcg aatggggcgg
     2701 caaggaatac aaacttgacg accaagagct tttgcttctt aaatttgacg acatcatggc
     2761 tgttatcaaa taacttttaa ttttacaaac tttataactt tataaactaa atctatggca
     2821 aaacaactaa aattcgggca ggacgcccgc caatctttac tcaacggtat taacactttg
     2881 gctgatgccg ttattaccac tcttggtccc aaaggccgca acgtcgctat cgacaaaaaa
     2941 tggggtggtc ccaccgttat tcacgacggc gtttctgtcg ccaaagaaat tgaattggaa
     3001 aacccttacg aaaatatggg cgctcagctt atcaaagaag ccgcttccaa aaccaacgat
     3061 attgctggtg acggcaccac cactgctacc gttttagctc aagctattgc caacaaaggt
     3121 ctccaaaacg ttgctgctgg tgccaaccct atgattatcc gtcgcggctt ggaaaaaggt
     3181 ttagctgccg tcatcaaaga gcttgacggt atgaaaaaag atatcaaagc cgatgataaa
     3241 gattccatcg agcaggtcgc cactatctct gccggcgatg ctgaaattgg caaacgcatt
     3301 gccgaagctg tcgttaaagt cggtcgcgac ggtctgatta ccgccgaaga aggcaagggt
     3361 ttatttttag aaaccaaaga aacttccggc atggaattcg accagggctt tttgtctccc
     3421 tattttgcca ccaacaccga aaccatggag gctattatcg acaaccctta cattgttatc
     3481 actgacaaaa aaatcagttc tatccaagaa attctgccgt ttttggaaaa gttggttaaa
     3541 ttgaccaaaa attttgtcat tattgccgac gatattgatg gtgaggcttt agctacttta
     3601 gttgtcaaca aaattcgtgg caccttcaac gttctggcag ttaaagctcc tggttttggt
     3661 gatcgccgca aagctatgtt ggaagatatt gccgtcctta ccaacggtca ggtaatttcc
     3721 gaagacttag gtatcaaatt tgataccgcc gaaccctcag attattgtgg ccgggctgat
     3781 tcagttactg ccgacaaaga caaaacccga attttgggtg gtgctggtgc cgaagtcgac
     3841 atcaaaaatc gggtttccca gctcagagct cagatgtcaa aaactgattc tgactatgac
     3901 caagaaaaat tccaagaaag aatttctaaa ttagttggtg gcgctatcgt ccttcaggtc
     3961 ggcggtgcta ccgaagtcga aatgaaagag agactagagc gggtcaaaga tgctatcgac
     4021 gctaccaaag ccgccgtcga agaggggatt ttgcccggcg gcggtgtggc tcttctcaaa
     4081 gcttctctta agcttgatga agtcaaagcc aacaatgacg aagaaaaagt tggtcttgat
     4141 attctcaagt atgccctgga gcagcccatt cgaagattgg ctcaaaactc aggcgaagat
     4201 gctggttata tttttaactt gatcaaagac aatgtcgcca aagatcccaa atcggattac
     4261 ggctacaacg ccgctaccgg caagatggaa tccatggtta aggctggcat tcttgaccct
     4321 gccaaagtta ctaaatcagc tcttaccaat gctgtttccg tcagcaccat gattttgacc
     4381 accgatgttc ttatcaccga tgctccggaa aaatcatctc ccgcttctcc tgctgccggc
     4441 atgggcggta tgggtggcat ggacggtatg atgtaagtta aatttgttaa ctaacaaaac
     4501 ccgccacccc ggcgggtttt ttgttaaaat aggcttactt gtttatccga cccggtagtc
     4561 aagcggttag acagcggtct gcaaaaccgt atagatgggt tcgactccca tccgggtctc
     4621 acttatttta gacagtcgta tcttgttata tcctataatt tgaaattatt taaattgttt
     4681 ggtagaatcc tttaatttat aaagcttata gctgcttttt taaattagca ctttttcagt
     4741 cagtcagaca aaaattcttg caattttaat tgcaattaat atattttttg tcggtttccc
     4801 aagaaaggag gtttctatgt ctacctgcgc tattattccc gaacacgatc tgacctctta
     4861 ccaagaattt ggcggtctgg aaacagggat tgccacgttt accaaatggg ttgacatccg
     4921 tgaccaaatg acccgaccac tgcgtcgcca aaccctcaac caagaccttc tcaagttcag
     4981 cgatcagctg gttttagatg gcgccgaccg aaaagacctt aaccgtattg ccggttggac
     5041 tgactgattt taacttctcg gcaggtgact gcatttttac taacctaaaa atgttttccc
     5101 tgcctttttt ttaactcctg tctttaaaat tctcccttct cccgctaaaa tgagtctatg
     5161 actcgccagc gggccccact cacttgtttt gtcggcccta tgaattccag caaaacccgc
     5221 cgtctgcttg atcacctctc cgaatgccgc gacgctctca actattccat cagggtctac
     5281 aaacccgccc ttgacaaccg gtttgatctc aacgaaatcc gttgtcgcaa cggtggctgc
     5341 tggacggcca ttccggttcc cagttccaaa cccagtcggg ccgattcctt gtttattttc
     5401 aaagatattt ataaattagc ctcccgccgc cgccccaatt tggtcgccat cgacgaaatt
     5461 cagttttttg acaaaaatat caaagaagtt gttaaatgcc tgctttacga taatattgaa
     5521 gtcgttgttt ccggtctcaa ccgaagcttc cgcggtgaac cttttcccgc cattgaatct
     5581 ttgctggctc tggctagcaa agtcgaaacc ctgactgccc gctgcactta caaagatggt
     5641 catggcaaaa cctgcggcgc tcccgccacc atgactcaac ggcttatcaa ccgccagccg
     5701 gcgccttact gcagtcctac tgttgtcatt cagcagattg gcgactcttc tgtcacctac
     5761 gaagcccgtt gcgccgatca ttggttttgt cccggcgttc ccatttccaa attaaaaaag
     5821 acctccaaat cttcccgtgg ctaatattat tgttgcccaa ccgcatggtt tttgttttgg
     5881 tatcaatcgg gccattgctc ttgcccggca aaccgcccaa aaattccacc aaccgatttt
     5941 tttcctcggt gaactggtcc acaatcaaca cgttgtcagt tggctggaac atgatttaaa
     6001 tataaaaacc gtcaccgaca ttaatcagat ttcccctaat tctatcgtta ttatccgcgc
     6061 ccatggcgcc gctcctcagg ttttccgccg ccttcggagt cgggggttaa ccttggttga
     6121 tgcttcttgt cctttggttc taaaatccca tcagactgtc cgccgtttag cctcagccaa
     6181 aaaatcaatc atctttcttt gcaacaaaat cgaccacgac gaaactgttg gtgttgttgg
     6241 tgaagctccc aagtttgtta ccccggtaac cttagacaat atttaccaac actccatttc
     6301 ccatcctgac caaaccgtgg ttattactca aaccactctt tctaccactg agaccaaagc
     6361 cgctcttgat tatctcaaaa acaaataccc tggtatcact attctgcccc acatctgtca
     6421 ggctaccacc aacagacaac aatcaattat caagctgacc aaaaagtatc attttgtcat
     6481 tattgttggt tctcctacca gcgccaattc caacagtctt cgccaagttg ccgaatctgc
     6541 tggcgctact gcttatattg tcgacaatcc taccgaactc aaacccgaat ggttccaaaa
     6601 tatcgataat attgttgtca gctccggtgc tagcaccccc gaagatattc ttttgtcggt
     6661 tattgatcaa atcaaagact ttactcgccc ataatttgga ctacaatttc ccgttcccgg
     6721 cagcaatcca actcaattgc aaaaattctc tgccataccc ccaaagtcat ttttccccct
     6781 atcaccggga ttgcctctga gtttctcatc attagttggg tgcagtgaga gtgtccgtta
     6841 agacagtccg attctcccga ctgacatacc aaattttctg ttcttatttt caaatcattg
     6901 tgacaataat acccgtcttt tggtaaaatt gtttttaaca ggtttctaaa atcctccaca
     6961 attccttttt ctttttcatt tatacaaatt gccactgtcg tatgttttga aaacaccaat
     7021 atttgccctt cttttatgtc gctgtctttc aaaaattttt ctatctcctc ggttatatcc
     7081 cgaaattcta aatattcttt ggtttcaaat ttgactatct tatgtttaat catagtgata
     7141 attaagtcgc ggcctttttc aaattcctgc gttattttct aactgctttc agcacccagt
     7201 agcctatata caacaacaat cctatctgca ccgccaccgc cgccccaaac caccagccca
     7261 catccttaat taccactccg tatcggtaat aatcccaaac tattcttgcc aaaaaatatg
     7321 agattatcgg cattaataat accatcacca ccaaaaatat ctgcgattta tcccggcttt
     7381 taattaggct ttttattacc actaccggct ctctgattaa gttataaagg ttgcacaaca
     7441 acttccatat ctgtctggcg gcaatcaaaa ttgttagttt taaaattttt ttaatccttt
     7501 ttattatcat aaacccaagc ttcccgaaat tgcttttttg cccttatcag ccttgactct
     7561 accgctttaa ttgttaggtt ggtttctttg gcaatttcgg ccactttttt ttcttctata
     7621 tacttaagtc gtagtaaatt tttatatcct gtttttaaat ccgacaaagt ctttttaatt
     7681 tcttttttta gttcattttt taatgcatcc cgttccgggg ttagagcctt gtcggcaatt
     7741 tcttcaaata ccgggctgac ggaaaataaa attgttttaa tctttttttt acggtagtag
     7801 tcaataattt tgtgattagc aatactgcaa acccaactaa attcactgca ctgccctcta
     7861 aaatttttcc acgaactcat ggctgccaac attacatcat tggtaatttc ttccaccact
     7921 ccttcatcat ctattttctg ccggataaag ttttttaccc ccaaactata ttttttgtag
     7981 cagtcttcca ttcccctgat tatatcttat tttccctttt tcaccctgcc ttgcgttttt
     8041 ttactggtaa ccgtattagc tttgctttat acttaattaa tgctcacacc taacctgccg
     8101 tattctcatc tcagccgcga tctccgtctc cagcttttgg atatgattta tgaatccggc
     8161 agcggtcatg ttggtggttg tctctcttca ctagacattc tcatcagcct ttatttttct
     8221 gatcttttta atttcaaccg agatcacttt atcttgtctg ccggccattt agcctgtgcc
     8281 ctctataccg tcctctctgc caaaaattac tttccttccc ggcttctttc cacctatacc
     8341 gattttaact ctctgctgca gggtcatgtt tctaccgatg ttcccggagt tgaatattcc
     8401 tccggttctt taggacaagg cttgtctttt gctgccggtc tggccctggg cgacaaatcc
     8461 cataccaccg tttgtcttac taccgacggc gaacatcagg aaggtcaaat ttgggaaagt
     8521 cttatgtttg cccaaaaata tcaactttca aatttaatca atattgtcga ctgcaacggc
     8581 acccaagtcg atggtcggat cgaagaaatt atgccgctta acaatttggc cgccaaatac
     8641 gtccaatttg gttggactgt caccactgtt aatggtcaca acctccatca aattgctaaa
     8701 gctcttaaat ccgctcttaa tcactctcac tccggctacc ccagttgtgt tattgccaaa
     8761 accgtccttg gtcggggagt ctcctttatg gaacatcagt ttaaatatca cgacattaaa
     8821 aatttagacc ccagtcttta tctccaagcc aaaaatgatc ttgaatccta ctaaaaccgc
     8881 ttctattcgt caggcctttg gtgacactct cacccaattg gctaaatcaa accgtcatct
     8941 ttacgtcgtt agcatggact taaaatcatc acttcatctg actaattttg ccaaaaaata
     9001 cccccgccgt tttatcgaat gcggtattgc cgaagccaat gccgccgcca ttgctgccgg
     9061 tctggccaaa tcgggcaaaa ctgtcttttt gtgttctttt gcttgtttct cgccggctat
     9121 caattgggcc gtcatccgcc aatccatttg ttacaatcac cttgatgtca aaattgttgg
     9181 ttctcatgcc ggcttaacta ctggagaatt gggtgccact catcagatgt tagaggacgt
     9241 ggctcttatg actacccttc ccaacatgga agtttttgcc cctgttgatg ctatcgaaac
     9301 tcaaaaaatc accaccaccc tcagccgcag ccgccgcccc gcttaccttc gtttagtccg
     9361 tcccgccacc agtcttttat ttgatcccaa aaatactttt accattggca aatcccatct
     9421 tctcaaatca ggccgtgata tcactgttat tggctacggt cctattcttg aacaggcttt
     9481 catggctcag tctcaactct ctctccaaaa acccaacatt tctttagaaa taatcaattg
     9541 ttcttccatt aaacctttcg atttcaccat cattagccgc agtctcaaaa aaaccggtcg
     9601 cctcatctgt cttgaagatc atcagcaaaa tgggggattg ggtcacttag tcgccgccgc
     9661 ccttctacat caccgacttt ctccaaaatt tattcatttg gccgtcaaca atcaatttgg
     9721 tcgttctgcc aagaattatt ctcagttata tgattattat ggtttaggtg ttaaaaattt
     9781 aattaccgcc gccaaaaaac ttctatgaaa tctctttctc aaatcttaac ctctgcccgt
     9841 cagcatcaaa ccgccattcc cgccttcaat attgactctt ttgaaatcta tcaggccgtc
     9901 gaatcggttg tcgccgacat taaccaaccc tgtatcgttc aattatcctc cggagaagat
     9961 agttttatcc aagccgaacg actttttatt ttagttcaaa aagctcgtct cgacggtctg
    10021 cccatttacc ttaatatgga tcatggcaaa gacagtcatc gtcttcttca tctcctccgt
    10081 cttggttttg atatggttca
//
LOCUS       DAOSHV010000010         9950 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_5785_length_9950_cov_4.926124, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000010 DAOSHV010000000
VERSION     DAOSHV010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 9950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9950
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5785_length_9950_cov_4.926124"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            355..1038
                     /locus_tag="PKZ29_00875"
     CDS             355..1038
                     /locus_tag="PKZ29_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37457.1"
                     /translation="MGEGSISPEINTEVKTPVLISPEAVKRLNGEGVRYGWEPLRIVD
                     EGRIAIARMPEGKSGIVIGSGPNTPEWKTRGWRTLDIDPQAGADMTLDANQLETMVPA
                     DSQDFLLAEYLTFDPLGKEGVTPARLLQQANRILKIGGELIIESVSFENLPESTIPNR
                     LEFAELMAKHGYDTVVEVGEYYHFGEDKKEQRVVYYGKKVASGFDGARAGSTKKETLI
                     YDPNTGKPL"
     gene            complement(1321..2328)
                     /gene="trpS"
                     /locus_tag="PKZ29_00880"
     CDS             complement(1321..2328)
                     /gene="trpS"
                     /locus_tag="PKZ29_00880"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002698334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOG37458.1"
                     /translation="MSKKPRILTGDTPSGKLHLGHYVGTLENRVKLQDTYDTYILLAN
                     FHAYANSYKQSDIINQNTYQVFLDNLAVGIDPHRSTIYLESGIPETFELYSLFLTMVT
                     HARAVRNPSVKEEINYKKMDPSLGFICYPILQAADILGFNADLVPVGEDQSPVIEQTR
                     EIARDFNSAFGPTFKIPKAMIGRVPRLIGTDGKTKMSKSGGNAILLSDDEAALKQKIQ
                     NCYTDPTRIHATDPGHVEGNPVFEYHRAFNPNREEVIDLETRYRAGQVGDKEVKDKLF
                     LALNKFLEPIRQKRKYYEDRPDLARDILIDGTRRARQVVQETLDLVKSKTDLNQLINK
                     I"
     gene            2440..3138
                     /locus_tag="PKZ29_00885"
     CDS             2440..3138
                     /locus_tag="PKZ29_00885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HOG37459.1"
                     /translation="MTKEKHQIKAAVRTVTGRKVKQIRKEGWLPATVYGKEFESLSIQ
                     FNLAEIVKLFDEIGESTLVEVILDEKEKLPVLFRNPQYHPIDGNMIHIDCYKVNLKEK
                     IKAMVPIELVGESQAVKDGNILVTVTDEIEVEGLPADLPEKIEVDLSALENLESTIIV
                     ADLKLDSDKLEILTDGEQLIAKVEEPKAEEAPVVEEANPEDVEVITQKEGKDEEGTDT
                     GNEEGEKEKEKKEE"
     gene            complement(3143..3586)
                     /locus_tag="PKZ29_00890"
     CDS             complement(3143..3586)
                     /locus_tag="PKZ29_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37460.1"
                     /translation="MKTKIVFLIFLIFLLTANTYRLLSVHDSYTQSKIKLSRFDGGIS
                     YLGRLNLWQSLVLSNDWEKAATLETKLDQTQISQYKADYQPQSLYTRLNQILNQSQKT
                     ADDYINIAKIYTLLDQTELSDDAIKKAHQLDPIRADIDRLFYQLP"
     gene            complement(3576..5870)
                     /locus_tag="PKZ29_00895"
     CDS             complement(3576..5870)
                     /locus_tag="PKZ29_00895"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOG37461.1"
                     /translation="MDKAYSSSRENDIYHLWEKSGAFTPKVPPNPKSVKTKHQPYSII
                     LPPPNANDPMHMGHALFTVQDILIRYHRMLGQPTLWLPGSDHAGIETQFVFEKKLAKE
                     GKSRFDFDRQTLYQLIWNFVEDNRKLNQFQMKQLGFSMDWTRYHYSLEPAIVKTALST
                     FTKLYHDGLIYRDEKIINYCPHCGTAFSNLEVDYVDEVTSLYYIKYGPFTLATTRPET
                     KFGDTAVAVNPEDKRYTQWVGQEITVDGLNGPFTVKVIADDMVDMKFGTGVVKITPAH
                     DPNDFEASLRHHLPLKKVIGFDGRLTDIAGKYAGLTVNQARDQVVADMKDRGMLVKID
                     DHYQHRIGRCYRCRHVIEPLVAPQWFIKISPLAKPAIQAAVDNQVKFFPSRFKKTYLD
                     WMKNIRDWNISRQIVWGPRIPAWYCLDCHPDIKVNFLDHHKNVVSGFYRDLKDNYSFA
                     EINSGLQSLIAPVGVDFIINSPTSCPKCHSHHMIQETDTFDTWFLSGQWPLTTLGFNP
                     DNPAKSSPDFDYFYPTTVMDTMWDILFFWVARMIMFGVYLTGQVPFKTVHLHSRVVDA
                     KGQKMSKSKNNVVDPLQMSQKYGSDALRMSLVYGIAPASDFVVSEDKIRAQRNFVNKI
                     WNASRFVEMIVDRFRQTNPRLKIDSKIDKSKLTSADKTILSRLNQIVASTTKNINSYH
                     FGQASENLYQFFWHEFCDIYIEDAKNRGEETIPVLLTVLQTSLKLLHPFIPFVTETIY
                     QELQSKFNFSPGLLITSSWPTHED"
     gene            5918..7006
                     /locus_tag="PKZ29_00900"
     CDS             5918..7006
                     /locus_tag="PKZ29_00900"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOG37462.1"
                     /translation="MKILDITKNYRKVIQQTAAVIKMGGLVVYPSDTVYILAVDPTNQ
                     TAVNKLLGFKNRWTGKAISVAVFDIKMAMEYVKIDENGKNIYANLLPGPFTIVGPGKH
                     RVAKGIEAENGTLGIRIPENKYVADLVKLVGGPVTATSANLSGRTPNYSVTSFLRPLS
                     GKKKEMIDMIVDAGKLPKNKPSTVIDITKPEIKVLRRGDLVTGKKVSLISKSEKETEK
                     IAEFLLKKVWDKLGKKPVVFLLSGDLGCGKTVFSRKIGKLLGVKEKITSPTFMIYNEY
                     PIKMSAKNKIDKFLHMDLYKITHKRDLAEIEFEEKFKRKNIICIEWPEQMGKNYLAKV
                     KKKSKVVGVNFEYVDENAREISYDLPKE"
     gene            complement(7007..7696)
                     /locus_tag="PKZ29_00905"
     CDS             complement(7007..7696)
                     /locus_tag="PKZ29_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37463.1"
                     /translation="MKTKNSTYLILTLVLLILSAIGAYFLYQLYQDRQSPAYNSATVP
                     KIELIPTDALEPEIIDIPDDIATPAAIVTASATKTITPTIYPTLIPTKAISPTSTPIT
                     TPTVSPATGSTQTYTSQTDNFSVAYAATRQFYQDTEPSGNRYTFTLSSGNFAVHVSAP
                     PQWSWTHPDREFTNTLLVSGKPTFKYETATQTIVDLQSSTKNYTIQCIHNGISALKTE
                     CTQFINSFKLL"
     gene            7725..8807
                     /gene="tsaD"
                     /locus_tag="PKZ29_00910"
     CDS             7725..8807
                     /gene="tsaD"
                     /locus_tag="PKZ29_00910"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOG37464.1"
                     /translation="MKILAIETSCDDTCAAVLDNDWVLSNIISSQTDLHAKWGGVVPD
                     IAKRAHSERIEPVIAEAILRSGILGSNKGKLKKEKIEEIILQGMKKIEVIGVTQGSGL
                     AIALGIGVNKAKELAKKYSKKLVAVNHIEGHILSNLVKNKNGNPQLELKLPALAMTAS
                     GGHTKLIWIEKIGEYKVVGETLDDAAGEALDKAAKMLGLGYPGGPVLERLAKIGKTDF
                     IKLPQPLADKKILDYSFSGLKTSFYYKIKDWPAEQIGTNLNNLAASFQNAVFETLLRK
                     FRLAAEKYQPRMLLVSGGVMANLDLRKRLRKMAKELDLPILMPTQKNLNTDNAAMIGV
                     AAYHKALRGEFVEDIDKLDRDPRMEI"
     gene            complement(8804..>9950)
                     /locus_tag="PKZ29_00915"
     CDS             complement(8804..>9950)
                     /locus_tag="PKZ29_00915"
                     /inference="COORDINATES: protein motif:HMM:NF016202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HOG37465.1"
                     /translation="TVVGFIPTQDQVNQTILRARRLIGKSINLLTDPDNITVTDQTLI
                     SWLGFDNNFDQEKILSYSQDLSNSLQRQPVDAVFKFERGQVLEFQPAKNGLTLDVNKL
                     AQEITSTLTNLETSDQTHLSVKLSYQSLPPKIKNSDVNDLGIVELLGSGTSTFKHSSN
                     TRNVNIVKGASIVNRILVAPGDTFSFVKSLGPVTIEAGYTKAYIIRQGRTELDVGGGI
                     CQVSTTLFRAMLNSGLDITERKAHAYRVSYYEEDSEPGFDATVFIPNPDLKFVNDTGH
                     YLLIQSLVDMDNRRLTYEIYGTSDGRKTTINNYRQWGYAPPPPTVWIDDPTLPVGKVI
                     KDETAVPGLKTSFDWTVTAADGRPLHQKTFTSNFVPWAAVYRRGPAN"
BASE COUNT         3217 a         1596 c         2576 g         2561 t
ORIGIN      
        1 aagagcaccg atcttataag tcgggggtcc atggttcgag tccatgtgca tccacaggaa
       61 tgaagaattg gggttgcctg gaagagtact cgccctgccc gcggcgagcg ctgtaaagcg
      121 tttatatata caaaaaagcg tcgcaaagga aaacgcaaaa ttttaaagaa ctaaaacttt
      181 aacgcccttg ctggtgagct cagacttgtt gaggcttgtc caatgagggc attaaaaatc
      241 tccacagtat tttgaactca aaaacaaagc ggaaaatgca atggtatttt ttctatgact
      301 tgattttcgg tcttattgta tacttatttt gagttatttt ttttaaaagg tgatatgggt
      361 gaaggatcaa ttagtccaga aatcaatact gaagttaaga ctccggtttt aatatcccct
      421 gaagcagtca agaggctaaa tggcgaggga gtgagatatg ggtgggagcc tttgagaatt
      481 gtagatgagg ggcgtattgc aattgcgcga atgccagaag ggaaaagtgg aattgtaatt
      541 ggttcaggcc ccaatacgcc tgagtggaaa acgagaggat ggagaacact ggatattgac
      601 ccacaagctg gtgctgatat gactcttgat gctaatcaac tagaaacaat ggtaccagct
      661 gattcccaag actttctgtt agctgagtat ttaacttttg acccattagg taaagagggt
      721 gttacacccg cgcgtctttt acaacaagcg aatagaattt taaagattgg aggagaatta
      781 attatagaga gtgtaagctt cgagaatttg ccagaaagta ccataccgaa caggttagag
      841 tttgcagagt tgatggctaa gcacgggtac gatactgtag ttgaagttgg agaatattat
      901 cattttgggg aagacaagaa ggagcaaagg gttgtgtatt atggaaaaaa agtagctagt
      961 ggttttgatg gggctagggc cggatcaacc aaaaaagaaa cactaatcta tgatccaaat
     1021 acaggaaaac cgttatagta acctgttaaa ccaggctttt tgaaatccat aaatatatag
     1081 aaactcagct acaactaacc aaacaatggc agtgatgata aatggtaaaa gatttttgaa
     1141 tgtaaaagtt gaaacagtat ttttgaaacc taaaaaaaga accggtaaaa aaaactaatc
     1201 cactaataaa agccatgaga ctaataacca gtaaaggatt gtattcgcta agatattttt
     1261 taatcaaaac accaccaagt gcgtaagaaa cttgggctaa aattaataaa accaaaccta
     1321 tcatattttg tttataagtt gattgagatc ggttttggat ttgaccagat ctagggtttc
     1381 ttggacaact tggcgggcgc ggcgggtgcc gtcgataaga atatcgcggg ccaagtcagg
     1441 ccgatcttcg taatattttc ttttttgacg aataggttcg agaaatttgt ttagggctaa
     1501 aaaaagtttg tctttgactt ctttgtcgcc gacttgaccg gcgcggtaac gggtttctaa
     1561 gtcaattact tcctctcggt tgggattaaa tgcccggtga tattcaaaaa caggattacc
     1621 ctcaacgtga ccggggtcgg tggcgtggat tcgggtggga tcggtgtagc agttttgaat
     1681 tttttgcttg agagcggctt catcatcgga aagtaaaatg gcgttgccac cgcttttgct
     1741 cattttggtt ttgccgtcgg tgccgataag acggggaacg cggccaatca tagctttggg
     1801 aattttgaag gtggggccaa aggcagaatt aaaatcacgg gcaatttctc gggtttgctc
     1861 aataaccggg ctttggtctt cgccaaccgg aaccaagtcg gcattgaaac caagaatatc
     1921 agctgcctga agaatggggt agcaaataaa acctaaagac gggtccattt ttttgtaatt
     1981 aatttcttct ttgacggagg ggtttctgac agcccgagca tgggtaacca tggtcaaaaa
     2041 taaggaataa agctcaaagg tttcgggaat cccggattca agataaatag ttgaacggtg
     2101 gggatcaatg ccaacggcta aattatccaa aaaaacttga taagtatttt ggttaatgat
     2161 gtctgactgc ttgtaagaat tggcataggc gtggaaatta gccaaaagaa tataggtgtc
     2221 gtaggtgtct tgaagtttga cgcggttttc gagagtgccg acatagtgac ccagatgaag
     2281 tttgccactg ggagtgtcgc cggtaagaat acgaggtttt ttggacataa acgattttat
     2341 cattaaatcg gaagctttat gatataatca gtttcagttt attaaggctg aaaccaggta
     2401 ggaccgaaag gaacaaaggg gttttggcaa aatgaaaaaa tgaccaaaga aaagcatcaa
     2461 ataaaagccg ccgtaagaac ggtgacaggc agaaaagtta aacaaatcag gaaagaaggt
     2521 tggttgccgg caacggttta tggaaaagaa tttgaatctt tgtcgattca gtttaatttg
     2581 gcagagatag tgaaattgtt tgatgaaatt ggagaatcaa ccctggtgga agtaattttg
     2641 gatgaaaaag aaaagctgcc agtgttgttt agaaatccgc aatatcaccc gattgacggc
     2701 aatatgattc atattgactg ctacaaggta aacttaaaag agaaaattaa agccatggtg
     2761 ccgattgaat tggtgggcga atcacaggcg gttaaggatg gaaatatttt agtgacggta
     2821 actgatgaaa ttgaagtgga gggtttaccg gctgatttgc cagagaaaat tgaagtagat
     2881 ttgtcggctt tggaaaacct ggaatcgacg attatagtag ctgatctgaa gttggacagc
     2941 gataagctgg aaatattaac agacggagaa caactgattg ccaaggtgga agaaccaaag
     3001 gcagaggaag ctccggtggt ggaagaagcc aaccctgagg atgtggaagt aatcactcaa
     3061 aaagaaggta aggatgaaga gggcacagac accggcaacg aagaaggaga aaaagagaag
     3121 gaaaagaaag aggaataaaa ctttatggca attgatagaa aagcctatca atatcggctc
     3181 taatggggtc gagttggtgg gcttttttga tggcatcatc agacaactca gtttggtcta
     3241 agagagtata aattttggcg atattgatgt aatcatcggc agttttttga gattgattga
     3301 gaatttggtt aagacgggtg taaagagatt ggggttgata atcagctttg tattggctga
     3361 tttgagtttg atctaacttt gtttccaaag tagcagcttt ttcccaatca ttggataaaa
     3421 caagggactg ccacaggttt aagcggccaa gataactaat gccgccatca aagcgggaca
     3481 attttatttt tgattgagtg taactgtcgt ggacggataa aaggcgatag gtattggcag
     3541 taagcaaaaa gattaaaaaa atgagaaata caattttagt cttcatgggt gggccaggaa
     3601 gaagtaatca acaaaccggg agaaaaatta aattttgact gcaattcttg ataaatagtt
     3661 tcggtaacga agggaataaa aggatgaagc aatttgagac tggtttgcaa aacagtaagg
     3721 agaacaggga tggtctcttc tcccctattt ttggcatctt cgatataaat gtcacaaaat
     3781 tcgtgccaaa aaaattgata taaattttca ctagcttgac caaaatgata agaattaatg
     3841 tttttggtgg ttgaagcaac aatctggtta agtcgggaaa gaatggtttt gtcagcggag
     3901 gttagttttg atttatcaat ttttgagtca attttgagac gggggttggt ttggcggaag
     3961 cggtcaacaa tcatttctac aaagcgggaa gcgttccaaa ttttgttgac aaaatttcgc
     4021 tgagctcgaa ttttgtcttc ggagacgaca aagtcgctgg cgggagcgat accataaacc
     4081 aaggacattc gcagagcgtc gctgccgtat ttttgggaca tttgcagcgg atcaaccaca
     4141 ttatttttac tcttactcat tttttgaccc ttggcatcga ccacccggga gtggagatgg
     4201 acagttttga aaggaacctg cccggtaaga taaacaccaa acataatcat ccgcgccacc
     4261 cagaaaaaga gaatatccca catagtatcc atgacggtag tggggtaaaa ataatcaaaa
     4321 tcgggagaag acttggcggg attgtcggga ttaaacccaa gagtggtcag cggccactga
     4381 ccgctaagaa accaggtatc aaaggtatca gtttcctgaa tcatatgatg agagtggcat
     4441 ttgggacaag aggtaggaga gttgatgatg aaatcaacgc cgacgggagc aatcagggac
     4501 tgaaggcccg agttaatctc ggcaaacgaa tagttgtctt tgagatcacg gtaaaaaccg
     4561 gaaacaacat ttttgtggtg gtccaaaaag ttaactttaa tatccggatg acaatcaaga
     4621 caataccaag caggaatccg gggaccccaa acaatttggc ggctgatgtt ccagtcacga
     4681 atatttttca tccaatcaag ataggttttt ttgaaacggg aggggaaaaa cttgacctga
     4741 ttgtcgacag cagcttggat ggcgggcttg gcaagagggc tgattttgat aaaccactga
     4801 ggagcaacca aaggttcgat aacatgacgg cagcggtaac aacgaccgat acgatgctga
     4861 taatgatcat caattttgac caacataccc ctgtctttca tatcagccac cacttgatca
     4921 cgggcttgat tgacagtcag accggcgtat ttaccggcaa tgtcggtaag ccgaccgtca
     4981 aagccaatga cttttttgag aggaaggtgg tggcggagac tagcttcaaa atcattagga
     5041 tcatgagcgg gagtgatttt gacaacgcca gtgccaaatt tcatatcgac catatcgtcg
     5101 gcaatgactt tgacagtgaa aggaccatta agaccgtcaa ccgtaatttc ctgaccgacc
     5161 cactgggtgt aacgtttatc ctcgggattg acagcaacgg cggtgtcgcc aaatttggtt
     5221 tcgggacggg tggtagccaa ggtaaaagga ccatatttga tgtagtaaag ggaagtgact
     5281 tcgtcgacat aatcaacttc aagattggaa aaagctgtgc cgcagtgggg acaataattg
     5341 ataatttttt cgtcgcgata gataagaccg tcgtgataaa gtttagtaaa agtggataaa
     5401 gcggttttga caatggccgg ctctaaggaa taatgatagc gagtccaatc catagaaaaa
     5461 cccaactgtt tcatttgaaa ttgattaagc ttgcgattgt cttcgacaaa attccaaatc
     5521 agttggtaga gagtctggcg gtcaaaatca aaccggcttt tgccctcctt ggccaacttt
     5581 ttttcaaaaa caaactgggt ttcgatgccg gcgtgatcgc taccgggaag ccacaaagtt
     5641 ggttgaccca gcatgcgatg ataacgaatg agaatatcct ggacggtaaa aagagcatga
     5701 cccatgtgca tagggtcatt agcgttgggc gggggcaaaa taatagaata aggctgatgt
     5761 ttggttttga ccgacttggg gtttggggga actttggggg tgaaggcacc agatttttcc
     5821 caaagatggt aaatatcgtt ttcccgggag gaactgtagg ctttgtccat ggagatattt
     5881 tagcacgaaa accggcggaa aatgataaaa tagggacatg aaaattttgg acataaccaa
     5941 aaattataga aaagtaatcc aacagacagc ggcagtgatt aaaatgggcg ggttagtggt
     6001 atatcccagc gacacggttt atattttggc agtcgatccc actaatcaaa cagcggtaaa
     6061 taaactgctg ggatttaaaa acagatggac tggcaaggca atttctgtgg cagtttttga
     6121 tataaaaatg gcgatggaat atgtaaaaat tgatgaaaat ggaaaaaata tttatgcaaa
     6181 tttattgccg ggtccgttta caattgtggg cccggggaaa catagggtag ctaaggggat
     6241 tgaggcagaa aatgggacgt taggaattag aattccggag aataaatatg ttgccgattt
     6301 ggtcaagcta gtaggggggc cggtaacagc aacatcagcc aatttgtcgg gaaggacacc
     6361 taattattcg gtgaccagtt ttttgagacc actgtcgggg aagaaaaaag aaatgattga
     6421 catgatagtg gatgccggca agctaccaaa aaacaaacca tcgaccgtga ttgatatcac
     6481 caaaccggag attaaggttt tgaggcgggg cgatttggtg accgggaaaa aagttagttt
     6541 aatatcaaaa tcggaaaaag aaacagaaaa aatagctgaa tttttattaa aaaaggtttg
     6601 ggacaaactt gggaaaaaac cagtggtatt tttactgtca ggagatttgg ggtgtggaaa
     6661 aaccgtcttt tccagaaaaa taggcaaact tttgggagtg aaagaaaaga ttacttcacc
     6721 cacattcatg atttacaatg aatatccgat caaaatgtcg gctaaaaata aaattgataa
     6781 atttttgcac atggatttgt ataaaattac tcataaaagg gatttggcgg agatagagtt
     6841 tgaggaaaaa tttaagagaa aaaatataat ttgtattgaa tggccggaac aaatgggaaa
     6901 aaattatttg gccaaagtaa aaaagaagtc aaaagtagtg ggggtaaatt ttgaatatgt
     6961 cgacgaaaac gcccgggaaa taagttatga tttgccaaaa gagtaattag aggagcttaa
     7021 aggaattgat gaattgggtg cactcggttt tgagagcgct gataccgttg tgaatacact
     7081 ggatggtata gtttttggtg gatgattgga gatcaacgat ggtttgagtg gcagtttcat
     7141 atttaaaggt aggtttgccg gaaactaaca aggtgttggt aaattcccta tccggatgag
     7201 tccaagacca ttgaggagga gcgctgacgt ggacggcaaa attgccgcta cttaaggtaa
     7261 aggtatagcg gttaccacta ggttcggtat cctgataaaa ctggcgggta gcggcatatg
     7321 ccacagagaa attgtcggtt tgactggtgt aagtttgggt gctgccggtg gcaggagaaa
     7381 ctgtgggagt ggtgatggga gtagaagtgg gagaaatagc tttggtggga attagggtgg
     7441 gataaatagt gggggtaata gttttggtgg cagaggcggt aacgatggca gcgggagtgg
     7501 caatatcatc gggaatatca ataatttcag gttcgagagc atcggtgggg ataagttcaa
     7561 ttttggggac ggtggcagag ttgtaagcag gtgattgacg atcctgatac aactgataaa
     7621 ggaaataagc gccaattgct gataaaatca gcagaactaa ggttaaaatt aaataagtgc
     7681 tatttttagt tttcataaat ttataattta ataatacatt attaatgaaa atattggcaa
     7741 tagagaccag ttgtgatgat acttgcgccg cagttttgga taatgactgg gtgttgtcga
     7801 acataatttc gtcacaaacc gatctgcacg ccaaatgggg cggagtggtg ccggatattg
     7861 ctaaacgggc tcacagtgaa agaatagaac cggtaatagc ggaagcaatt ttgagatcgg
     7921 gaatattggg gtcaaataag ggaaaattaa aaaaagaaaa aatagaagaa ataattttgc
     7981 aaggaatgaa aaaaattgag gtaattgggg taactcaagg gtcggggttg gcgatagctt
     8041 tggggatagg agtaaataag gctaaagaat tggcaaaaaa atatagcaaa aagttggtgg
     8101 cagtaaacca cattgagggt catatattgt caaatttggt aaaaaataaa aacggcaacc
     8161 cacaactgga gctaaaactg ccagcgttgg caatgacggc ttcggggggg catacaaaat
     8221 taatctggat agaaaaaatt ggcgaatata aggtggtggg agaaaccttg gatgatgcgg
     8281 ccggggaggc actggacaaa gccgccaaaa tgctggggtt aggttatccg ggaggaccag
     8341 tgttggaaag actggcaaaa atagggaaaa cggattttat aaaactaccc caacctttgg
     8401 cagacaaaaa aattttggat tatagttttt cggggttaaa aaccagtttt tattacaaaa
     8461 taaaagattg gccggcagaa caaatcggga ctaatctaaa taatttggcg gcaagttttc
     8521 aaaacgctgt gtttgaaact ttattgagaa aatttaggct ggcggcggaa aaatatcaac
     8581 caaggatgct gcttgtcagc ggcggagtga tggcaaattt ggatttgaga aaaagattga
     8641 gaaaaatggc caaagaattg gatttgccaa tattaatgcc gacacaaaaa aacctaaaca
     8701 ctgataatgc cgccatgatt ggggtggcag cttaccataa agccttaaga ggtgaatttg
     8761 tggaagatat cgataaactt gatagggatc cgagaatgga aatttagttg gcgggaccgc
     8821 ggcggtagac agccgcccag gggacgaagt tggaagtgaa agttttttga tgaagaggac
     8881 gaccgtcggc agcggtgacg gtccagtcaa aggaagtttt gagtccgggg acggcggttt
     8941 catctttgat aaccttgccg acaggaagag tgggatcgtc aatccaaact gttggcggcg
     9001 gcggggcata accccactgg cgatagttat taatagtggt tttgcgacca tcggaggtgc
     9061 cataaatttc ataggttaga cgccggttat ccatatctac caggctttgg ataagaagat
     9121 agtggccggt gtcattgaca aacttaaggt cggggttggg aataaaaaca gtagcgtcaa
     9181 aacctggctc actgtcttct tcatagtaac taacccgata agcatgggct tttctttccg
     9241 taatatctag gcctgaattt aacatggccc gaaagagagt ggtggagacc tgacagatac
     9301 cgccaccgac atcgagttcg gttcgaccct gacgaataat ataagctttg gtgtaaccag
     9361 cttcgatagt aaccggacca agagatttga caaaagagaa agtgtccccg ggagcgacta
     9421 aaatccgatt aacaatggag gcgcctttga caatattaac gttgcgagta ttgctggagt
     9481 gtttaaaagt ggaagtgccg ctgcccaaaa gttcgacaat accaagatca ttaacatcgc
     9541 tattttttat ttttgggggc agggattgat aagaaagttt aacactaaga tgggtttgat
     9601 cagaagtctc taaattagta agagtggaag taatttcttg ggcaagttta ttaacgtcaa
     9661 gagtcaagcc atttttggcg ggttgaaatt ctaaaacctg accacgttca aacttgaaaa
     9721 cagcatcaac cggttggcgc tggaggctgt tggagagatc ctgagaataa gaaagaattt
     9781 tttcttgatc aaaattattg tcaaaaccca gccaggaaat taaggtctga tcggtaacgg
     9841 tgatattgtc tgggtcggtc aaaagattga tggatttacc aataaggcga cgggcgcgaa
     9901 gaatagtttg attaacctga tcctgagtgg gaataaagcc gacgacagta
//
LOCUS       DAOSHV010000011         9937 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_5800_length_9937_cov_5.597551, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000011 DAOSHV010000000
VERSION     DAOSHV010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 9937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9937
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5800_length_9937_cov_5.597551"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="PKZ29_00920"
     CDS             <1..581
                     /locus_tag="PKZ29_00920"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF013296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HOG37466.1"
                     /translation="KDSHRLLHLLRLGFDMVHFDGSSLDYSQNLSQTAEFIAAAKNIR
                     PDCLIEAEFNRINLVDSQISPDSFTQPSQAAEFIAKTGADLLAVSIGNLHGVSQKSVE
                     TIDLDLLSQIKSALPQQFLTLHGGSGISSDQLNSAINMGVVKININTDLRRQFFQSLK
                     ANLSQYSGDKIYQFFDPVITDLKSVIKSKFHV"
     gene            574..1494
                     /locus_tag="PKZ29_00925"
     CDS             574..1494
                     /locus_tag="PKZ29_00925"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HOG37467.1"
                     /translation="MFDVVSIGAATVDIFVKSDALTATPDIIGLTPSSKNEINSSLIC
                     SGGGATNSSVAFSRLGLKSACVSLIGHSYLNNYIIDDLKREKVSDKFICSNKKDTTDF
                     SVILVSKDGRRSILTNRGKSGLTDKNFRWSQLKSIKWFYITSLEGNLDLLEKIIGFAK
                     ENQIRLSLNPGNRELARRRRLISLLKYVDFLLLNRHEAETLLDLSFSDADFFNQLHSL
                     KSSLIAVTDGRQGAYLIDRNQQYFSPIINTTPVDETGAGDSFGSAFVAATIYRQPPQE
                     ALFWAIKNSASVVTRLGAKAGLLTLKQIKS"
     gene            1497..2144
                     /locus_tag="PKZ29_00930"
     CDS             1497..2144
                     /locus_tag="PKZ29_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37468.1"
                     /translation="MLPKPEKSKKLTNLLAEDKASTESEAEISQKRLLLKRRLILISL
                     LMTAGISFIFWSFRAAQKIIHQPPQFNLSFKFPRPNFSLKNNPSTNQTDIFVLSDSQS
                     SWSIASVAKSRPDKPVFSHNYNNFNFDQTMTSLSFLKPSSSSLINLQLPQGLTFQEKT
                     ASENNSLIYQNLISLPSDTLLISVLIPQTDNLESVKSQLSGLVESLYWYSVRNLN"
     gene            complement(2137..4338)
                     /locus_tag="PKZ29_00935"
     CDS             complement(2137..4338)
                     /locus_tag="PKZ29_00935"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HOG37469.1"
                     /translation="MTGIESIIKFLIKLGWPVVKVVKFLTTGVKRTVKKIKNIKLRLP
                     KVKLPKIKPISFRLPKIKFKRKLKHKIQRPKKELKSKRKFKKSFWRKIFIGIGIVIIA
                     GAAGGWIWTNIFRDLPNVNLIYNPPKLSTIITDRNGQVLYKFYEDENRTWVPLEKIPK
                     DLIEATLAIEDKKFYKHHGLSLKGLMVAVRYNFKKETEDKPRGGSTITQQLVKNVFLG
                     NEKTFLRKIKEAVLTIMVEQKLSKDEILERYLNQVSYGGEAYGAEAAAQKYFGKSVTK
                     INTIEAAYLAGLPAAPSSYSPYGNNPELGLLRAKRVIEEMVSNKYLTEVQAEQWLREK
                     INIKNSNRQILAPHFVFYIKNLVQEKYGFTNIEKQGLIIKTTLDLKTQAMAQEIVRQE
                     INNISRLNVTNGAAVVIEVKNGDILAMVGSKDYLATDIDGKYNVTTALRQPGSSIKPI
                     NYLLALENGYSMASRIDDSPITYQIKGQKPYTPQNYNGKYMGQVSLRTALGSSLNIPS
                     VKLLEKNGVENMIDLGEKMGINSWHDRKRFGLSLALGGGEVKMTELSQAYSVLANLGE
                     KVEINPILEIDNYLGEKIYVKEVEKERVVRAEYAYIINDVLSDNQARTPIFGANSKLV
                     IKDKTVAVKTGTTNSLKDNWCIGWTPTYLVAAWVGNNDSQPMSWVASGVTGATPIWNK
                     IMTEMIGKKENEIWEPPKSLIKKRVCGKEEWLIEGSEIKVNCPVITPTPTN"
     gene            complement(4338..>5063)
                     /locus_tag="PKZ29_00940"
     CDS             complement(4338..>5063)
                     /locus_tag="PKZ29_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37470.1"
                     /translation="MGIRQDLNPIMKVVDKNGKTLEEYEYNPGPRVLSRETSYIIQQI
                     LSDDGARAMVFGRGSMLNIKGHPEVAVKTGTTNDMRDNWTIGYTQDYTVTTWVGNNDN
                     SKMGNLVSGTTGAAPIWNKIMTQLLTGKEIKQPTRPAGIVGMNVCNLTGMAIPEGGCE
                     SHYEYFKKEFVPGKQAPVKQTVVVNKDTGRMVVDNEEAPNAEWQEHLVIEDASGVKIC
                     LDCQPVEQNPDGSPIKIPAALIR"
     assembly_gap    5064..5163
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5164..6664)
                     /locus_tag="PKZ29_00945"
     CDS             complement(<5164..6664)
                     /locus_tag="PKZ29_00945"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HOG37471.1"
                     /translation="MSKTRKKTKKGRRRWLAIVLIALMVIGIGIIIFTLFNVPSPKKL
                     SSGEYAQSSQIFDRNGELLYEIYAEKRRLPVKLDEVPENLKKATLAIEDANFYKHGGF
                     DIKGLARGLYRTIIEKRLQGGSTLTQQLVKNGLLTPERTLSRKFKEAVLTVATEVMYS
                     KDEILEMYFNQTPYGGTLWGVQSAANGIFNKEVKDLTLAESALLAGLPASPTKYSPFS
                     YPETAKARQMLVLERMKELKMISQDEYDKAKEEHLNYYVNKAQIKAPHFVFWVKNLLM
                     QKYGIKKVTEGGLKITTTLDMEIQKMAEATVSAELTKLEKYKVSNGAALITEPKTGQI
                     LAMVGSVDYFSDKIDGKYNVTTALRQPGSSIKPINYAVGIETGKITAASVIYDNPTCF
                     AQPGQRNYCPTNYGGAYHGLQNIRTSLASSLNIAAVKVLKINGLETFVASASAMGLST
                     FKNASDYGLSLTLGGGEVTMTDMATAYGVLANMGIRQDLNPIMKVVDKNG"
     gene            complement(6661..7182)
                     /locus_tag="PKZ29_00950"
     CDS             complement(6661..7182)
                     /locus_tag="PKZ29_00950"
                     /inference="COORDINATES: protein motif:HMM:NF039823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5667 domain-containing protein"
                     /protein_id="HOG37472.1"
                     /translation="MKLWFKISMVVISLVILYVSFARAGLEKVSQDEKYDQLRKISIA
                     YHQGSECYKLPESRTLPDNPFYFLKKIRDDLWIQFTNNPIDRVRIGLLVSDKKITEAI
                     MLYRLGKKEAGWKNNLAEAGVKNLKLREEMAKLNGEDIEVQKLITKVDRANSFYQDII
                     EQLSKNEPIKECL"
     gene            complement(7179..7952)
                     /locus_tag="PKZ29_00955"
     CDS             complement(7179..7952)
                     /locus_tag="PKZ29_00955"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOG37473.1"
                     /translation="MKAETAVKKTINYAAGFKSHINKKEVNQRLISAKKFSKKDIDMV
                     LKKMGWRDKKNKWYRGKVEMINELAKKMETEFRDIYFLGITGSVASGHPKKGDDIDIM
                     IITKNNKLWKNRLKLRWWMKQNQLPYRDRRGKRKENCFCFNLWLDESGLKIPAKKQNL
                     RNAVDLILMKPIINKNKSYEKFLKENSWAKKYLASGYDQLEKKYNFKKVKMKRFKGGD
                     MGNKFYFWPQYWYMKPKMTNENVGLHQAFFHKSDGKIES"
     gene            8001..9509
                     /locus_tag="PKZ29_00960"
     CDS             8001..9509
                     /locus_tag="PKZ29_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37474.1"
                     /translation="MVNKNTRRYRLWEMIPGFFAWMTILFPIWGAIFFPKAVAYFVIA
                     FLLYWLYQSFKSAILALIGYFKIRLATKTNWLELFQDKFLASWLKYKQIHHVIIISSY
                     KEPVEVIEMAVGSLAAQIDIDLKKLHLILAQEQRAGKENNQKTIDYFSQKYRGVFGDF
                     IFTEHPPDIPGETAGKHSNERFAAKFFKQKYIDTGKYDINHLTLTSCDVDTIFNPKYF
                     SALTYNFASNPSRYYRFWQSPIFWHNNINQVPSLIRMTGVLGNVIHIANIQEPDGLFF
                     NYSCYSSSFHLIDHSGYWDPDMIPEDWHIFLQAFFATSGKALVEPIFLPTVVDAPDGK
                     NYFDALKNRYSQCVRHAWGAIDIPYAIEQSRQHPEIPLVTRALRIFKLIQTHLIWSSN
                     WFILTLGTSLPVILNPKFFQTTFGFNLPRISNIILTICLIPLAILIILDWKLRPPRQK
                     KGFLNIVKNILQWPLMPIATLTMSVLPGLHSHTRLFLGRKLDYKTTNKKAVK"
     gene            9524..>9937
                     /locus_tag="PKZ29_00965"
     CDS             9524..>9937
                     /locus_tag="PKZ29_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37475.1"
                     /translation="MTKFLSRHKYWVFVIILFIFLRLPSLFEPYWYGDEGIYLTLGQA
                     ITKGDILYRQIHDNKPPTLYYLAAISQTVFGFRLLLALFMVPTIYFFYRLCLYFFTSK
                     TARISLLFFVILTSLPTLEGNIANAEIFMLLPTILA"
BASE COUNT         2404 a         2470 c         1453 g         3510 t
ORIGIN      
        1 gcaaagacag tcatcgtctt cttcatctcc tccgtcttgg ttttgatatg gttcattttg
       61 acggctcttc tcttgattat tctcaaaatt tatcccaaac tgccgaattt attgccgccg
      121 ccaaaaacat ccgtcctgat tgtttgattg aagccgaatt taatcgcatc aatttagttg
      181 acagccaaat ttcacccgac agttttaccc aaccttctca ggcggcagaa tttatagcca
      241 aaaccggcgc cgatttgctg gctgtttcca tcggtaatct tcatggtgtc agtcaaaagt
      301 ctgtcgaaac cattgacctt gatctcctgt ctcagatcaa atccgctctg ccccagcaat
      361 ttctcactct ccacggcggt tccggcatta gctccgacca attaaattct gccataaata
      421 tgggagttgt caaaattaac attaacaccg atctccgccg ccagtttttc caatctctta
      481 aggccaattt atctcaatat tccggcgata aaatttatca gttttttgat ccggttatca
      541 ccgatcttaa atcagttatt aagtcaaaat tccatgtttg atgttgtttc tatcggcgcc
      601 gccaccgtcg atatctttgt caaatccgac gctctcaccg ctactcccga catcatcggt
      661 ctcaccccct cctccaaaaa tgaaattaat agcagtctta tttgtagcgg cggcggcgct
      721 accaattcct cggttgcttt cagccgtctt ggtctcaagt cagcttgtgt ttctcttatc
      781 ggccacagct atcttaacaa ttatattatc gacgatctta aacgggaaaa agtcagtgat
      841 aaatttattt gttcaaacaa aaaagatact accgatttct ccgttatttt agtctccaaa
      901 gatggccgcc gttccatcct taccaaccgg ggaaaatctg gtttaaccga caaaaatttt
      961 cgctggagcc agcttaagtc aatcaaatgg ttttatatta cttctcttga gggcaatctg
     1021 gatttgttag aaaaaatcat tggttttgcc aaagaaaatc aaatccgcct ttctcttaat
     1081 cctggcaacc gggaattggc ccgccgccgc cgtttaattt cgcttctcaa atatgttgac
     1141 tttttacttc tcaaccgtca cgaagccgaa acccttttag atttatcttt ctccgatgct
     1201 gactttttta accaactcca ttctcttaaa tcatctttaa ttgccgtcac tgacggccgt
     1261 cagggagctt acctcataga tcgcaaccag cagtattttt ctcccatcat caatactacc
     1321 cctgtcgatg aaaccggcgc cggcgactct tttgggtctg cctttgtggc tgctaccatt
     1381 taccgccagc cgccccaaga agcccttttt tgggctatca aaaactccgc ttccgttgtt
     1441 acccgtcttg gcgccaaagc cggtctttta acccttaaac aaatcaaatc ctaaatatgc
     1501 tccccaaacc ggaaaaatcc aaaaaactga ccaatctttt ggccgaagac aaagcctcaa
     1561 ccgaatccga agccgagatt tctcaaaaac gtcttcttct caaacgccga cttattttaa
     1621 tctctctttt gatgaccgct ggtatcagtt ttatcttttg gagttttcgc gctgctcaaa
     1681 aaattattca tcaaccgccc caatttaatc tcagttttaa atttccccga ccaaattttt
     1741 ctctcaaaaa taatccttct actaatcaaa ccgacatttt tgttctctct gactcccagt
     1801 ccagctggtc tattgcctct gttgccaaat cccgccccga taagcctgtt ttttcccaca
     1861 attacaataa ttttaatttt gatcagacca tgacttctct ttcttttctc aaaccttctt
     1921 cttctagttt aattaatctc caacttcctc aaggtctaac ctttcaggaa aaaactgcct
     1981 ctgaaaataa cagtttaatc taccaaaatc ttatctcctt accttccgac actctcctta
     2041 tttctgttct tattcctcaa accgacaatt tggaatcagt caaatctcaa ctctcaggtt
     2101 tagttgaatc tctttattgg tattccgtta gaaaccttaa ttagttggag ttggcgttat
     2161 taccggacag tttaccttaa tttcgctgcc ttcgatcaac cattcttctt tgccacaaac
     2221 tctctttttt atcaaacttt ttggtggctc ccatatttca ttttctttct tcccaatcat
     2281 ttccgtcatt attttattcc atatcggtgt tgctcccgtc actcccgaag ccacccaact
     2341 catcggttgg ctgtcattat ttcccaccca agctgccacc agatatgtcg gcgtccagcc
     2401 aatacaccag ttatctttca gactgtttgt tgtcccagtc ttgacggcta ctgttttatc
     2461 ttttattact agttttgagt tagctccaaa aataggggtt ctggcctgat tgtcgctcaa
     2521 aacatcattg ataatatagg cgtattctgc tctcaccact ctttcttttt ccacttcttt
     2581 aacataaatt ttttctccca gataattatc gatttccaaa attgggttta tttctacttt
     2641 ttctcccaag tttgccaaca cgctataagc ctgagacagt tccgtcattt ttacctcgcc
     2701 tccgcccaaa gccaatgaca atccaaatct ttttcggtca tgccacgaat ttattcccat
     2761 tttctccccc aaatcaatca tattttccac tccgtttttt tccaataatt ttactgacgg
     2821 aatattcaaa gacgatccca gcgccgttct taaactcact tgacccatat atttgccgtt
     2881 ataattttgc ggcgtatatg gtttttgtcc ttttatctga taagttattg gtgagtcgtc
     2941 aattcttgat gccatactat aaccattttc caaagccaac aaataattta tgggtttgat
     3001 cgacgacccc ggttgtctta gggcggttgt tacattatat ttgccgtcaa tatccgttgc
     3061 caaatagtcc tttgatccca ccattgccaa aatatccccg ttttttacct caattaccac
     3121 tgccgctcca ttggttacat taagtcggga aatattattt atttcttggc ggactatttc
     3181 ttgtgccatt gcctgagttt tcaaatccaa cgttgttttg attatcaacc cctgtttttc
     3241 gatattggta aacccgtatt tttcctgcac caggtttttt atataaaata caaaatgcgg
     3301 cgccaagatc tgtctgttgc tgttcttgat atttattttt tcccttagcc actgttccgc
     3361 ctgaacttca gtcaaatact tatttgatac catttcttca attacccgtt ttgctcttag
     3421 caagcccaat tctgggttgt ttccgtaagg tgaataagaa cttggcgctg ccggtaaccc
     3481 cgccaaataa gccgcttcaa ttgtattgat tttagtcacg cttttgccaa aatatttttg
     3541 cgccgccgcc tccgctccat aagcctcgcc gccatatgac acctggttta agtatcgttc
     3601 caaaatctca tctttactca atttttgctc taccataatt gtcagcaccg cctcttttat
     3661 ttttctcaaa aaagtttttt cgtttcctaa aaatacattt ttaaccaatt gttgggtgat
     3721 agttgatcct cctctgggtt tatcttccgt ttcctttttg aagttatatc gcactgccac
     3781 cattagtcct ttaagcgata acccgtgatg tttataaaat tttttatcct caattgccag
     3841 cgtcgcttca atcaaatcct ttggtatttt ttccaacggc acccaagttc tgttttcatc
     3901 ttcataaaat ttatacaaca cctgtccgtt tctgtctgta attatcgtcg acagtttggg
     3961 tggattataa atcaggttga catttggcaa atccctaaaa atgtttgtcc aaatccatcc
     4021 acccgctgct cccgctataa tcactattcc tattccgata aatatcttcc tccaaaaact
     4081 ttttttaaat tttctttttg attttaactc tttttttggt ctttgaattt tatgttttag
     4141 tttccgttta aatttgattt tgggtagtct aaaacttatc ggttttatct ttggtagttt
     4201 tacttttggc aggcggagtt taatattttt tatttttttg actgtccttt ttacaccagt
     4261 agtcaaaaat ttaaccacct tcaccaccgg ccatcctagt ttaattaaaa attttataat
     4321 cgattctatt cctgtcatta tctgattaag gcggcaggga tttttatggg agaaccatct
     4381 ggattttgct ctactggctg acaatcaaga cagattttaa ctcccgaagc gtcttctatc
     4441 accaaatgtt cttgccattc agcatttggg gcttcctcat tgtccaccac cattcttccc
     4501 gtatctttat tgaccaccac cgtttgtttt accggagctt gttttcccgg aacaaattct
     4561 tttttaaaat attcataatg agactcgcat ccgccttccg gaattgccat tcccgtcagg
     4621 ttgcacacat tcattcccac aatccccgcc ggtcttgtcg gttgtttaat ttcttttccc
     4681 gtcagcaatt gagtcatgat tttattccaa atcggcgccg ctcccgttgt tcctgacacc
     4741 aggttaccca tttttgaatt atcgttgttt cccacccaag tcgtcaccgt ataatcttga
     4801 gtatagccga ttgtccagtt atctctcata tcattggttg tccctgtttt taccgctacc
     4861 tccgggtgtc ctttaatgtt caacatcgac ccccggccga acaccatcgc cctagccccg
     4921 tcatccgaca aaatctgttg aattatataa cttgtttccc gtgacaacac tcttggtccc
     4981 ggattatatt catattcctc caaagttttt ccgttcttat ctaccacttt cattattgga
     5041 ttcaaatctt gtctgatacc catnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5161 nnnttccgtt cttatctacc actttcatta ttggattcaa atcttgtctg atacccatat
     5221 ttgctaatac tccataagcg gtcgccatat cggtcattgt cacttcacct ccgcccaaag
     5281 tcaacgacaa accataatcc gaagcatttt taaatgtgct caatcccatg gctgatgccg
     5341 aagccacaaa ggtttccagc ccatttatct ttaatacttt cactgctgca atattcaaag
     5401 agctagccaa agaagttctt atgttttgta gtccatgata agccccgccg taatttgtcg
     5461 gacaataatt tctttgccct ggttgagcaa aacaggtggg attatcataa atcaccgacg
     5521 ccgccgttat cttgcccgtt tctattccca ctgcataatt tataggtttg attgacgacc
     5581 ctggttgtct tagggcggtt gtcacgttat atttaccatc aatcttgtcc gaaaaataat
     5641 caaccgatcc caccattgcc agtatctgtc ctgtctttgg ttcggttatc aatgccgctc
     5701 cattgctgac tttgtatttt tccagtttag tcagttctgc tgatactgtt gcctctgcca
     5761 ttttttgtat ttccatatcc aaagtcgtcg taattttaag cccgccttca gtcacttttt
     5821 tgattccata cttttgcatc aacaaattct tcacccaaaa cacaaagtgc ggtgctttaa
     5881 tctgagcctt gttgacataa taattcaaat gctcctcttt agctttgtca tattcgtcct
     5941 gactaatcat ctttagttct ttcatccgtt ccaacaccag catctgcctt gcttttgctg
     6001 tctccggata agaaaaaggg gaatatttgg ttggtgatgc cggcagtccc gccaataggg
     6061 ccgactctgc cagtgtcaaa tccttaacct ctttgttaaa aattccgtta gccgccgact
     6121 gcacccccca cagcgtccct ccgtagggag tttggttaaa atacatctcc aaaatttcat
     6181 ctttagaata cattacctct gttgccacgg ttaatactgc ctctttaaat tttctcgata
     6241 gagtcctttc cggtgttaac agtccgtttt taaccagctg ttgggttaga gttgaccccc
     6301 cctgcagtct tttttctata atcgtccgat acaaacctct tgccagtcct ttaatatcaa
     6361 atccgccatg tttataaaag ttagcatcct caattgccag cgttgccttt tttaggtttt
     6421 ctggcacttc atccaatttt actggcagcc ttcttttctc ggcataaatt tcatacaata
     6481 attctccgtt ccggtcaaaa atttgtgatg actgagcgta ttcaccacta gacaattttt
     6541 tagggctggg aacattaaac aaagtaaaaa ttattattcc tattcctatc accatcagag
     6601 caatcaaaac tatcgccagc caacgccgac gtcctttttt cgtctttttt ctggttttac
     6661 tcataaacac tcctttattg gttcattttt tgataactgt tcgattatat cctgataaaa
     6721 agaatttgcc ctatcaactt tggttatcaa tttttggact tcaatatctt ccccattaag
     6781 ttttgccatt tcctccctca acttcaagtt tttaactcct gcttccgcta aattattttt
     6841 ccacccagcc tcttttttcc ccaacctgta taacattatc gcctccgtta tttttttatc
     6901 cgataccagc aaccctatcc ttacccgatc tatcggattg ttggtaaatt gaatccacaa
     6961 atcatctctg atttttttta aaaaataaaa aggattatcc ggcagagttc gactctccgg
     7021 cagtttataa cattcgcttc cttggtgata ggcgattgat atttttctta gctggtcata
     7081 tttttcatct tggctgactt tttccagtcc tgccctggca aacgatacat ataatattac
     7141 caacgatatc actaccattg atatcttaaa ccacaatttc atgactctat tttaccatct
     7201 gacttgtgaa aaaacgcctg atgtaaccct acattttcat tagtcatttt tggtttcata
     7261 taccaatatt gcggccaaaa ataaaattta ttgcccatat ccccaccttt aaatcttttc
     7321 atttttactt ttttaaaatt gtattttttt tccaactggt cataacctga ggccaaatat
     7381 ttcttcgccc agctattttc cttcaaaaat ttttcatagc ttttattttt atttattatt
     7441 ggtttcatca aaatcaaatc caccgcattt cttaaatttt gttttttggc aggtattttt
     7501 aaccctgact catccagcca caaattaaaa caaaaacaat tttcttttct ttttccccgc
     7561 cggtcgcggt agggaagttg attttgcttc atccaccacc taagttttaa tctgtttttc
     7621 cataatttat tatttttggt gataatcata atatcgatat catcgccttt tttaggatga
     7681 cccgaagcca ccgacccggt tattcccaaa aaataaatat ctctaaattc agtctccatt
     7741 ttcttggcca actcatttat catttccacc ttgccccgat accacttatt ttttttatcc
     7801 cgccaaccca ttttttttaa caccatgtct atgtcttttt ttgaaaattt ctttgccgat
     7861 atcaatctct ggttaacctc ttttttattt atatggcttt taaatccggc cgcataatta
     7921 atagtttttt tgactgctgt ttctgctttc acatcctatt ttatcccaaa attaatctct
     7981 cttgatataa ttacaagacc atggtcaaca aaaacaccag gcgttatcgc ctctgggaga
     8041 tgatccccgg attttttgct tggatgacaa ttcttttccc tatttgggga gccatatttt
     8101 tccccaaggc cgtcgcttat tttgttattg ctttccttct ttattggctt tatcagtcat
     8161 ttaaatctgc cattcttgct ctcatcggct atttcaaaat taggcttgct accaaaacta
     8221 attggctgga attgtttcag gataaatttt tagcctcttg gctaaaatac aagcaaatcc
     8281 atcacgttat tattatttcc tcctacaaag aaccggttga agttatcgaa atggccgtcg
     8341 gcagtcttgc tgctcaaatt gatattgatc tcaaaaaact tcatcttatt ctggcccaag
     8401 aacagcgggc cggcaaagaa aataaccaaa agactattga ttatttttcc caaaaatatc
     8461 gcggcgtttt tggcgacttt atttttaccg aacaccctcc tgatattcct ggtgaaaccg
     8521 ccggtaaaca cagcaacgaa cgctttgccg ccaaattttt caaacagaaa tatattgata
     8581 ccggcaaata tgatattaat catctcactc tcactagttg tgatgttgat actattttta
     8641 atcccaaata tttttctgcg ttgacttaca attttgcctc caatccttcc cgctattacc
     8701 gcttttggca gtcgcccata ttttggcaca acaatatcaa ccaggtcccc tctctcatcc
     8761 gcatgaccgg agttttgggc aacgtcattc acattgccaa cattcaggag cccgacggtc
     8821 tcttttttaa ttattcctgt tattcttcct cctttcatct aattgatcac agcggttatt
     8881 gggaccctga tatgattccc gaagattggc atatttttct tcaggccttt tttgccactt
     8941 ccggcaaagc cctggtcgaa cctatctttt tgcccaccgt tgttgatgct ccggacggta
     9001 aaaattattt tgatgctctc aaaaaccgct acagccaatg tgtccggcat gcttggggag
     9061 ctattgatat cccttatgcc atcgaacagt cccgtcagca ccctgaaatt cccctcgtta
     9121 cccgggccct acgtattttt aaacttattc aaacccatct catttggtcc agtaattggt
     9181 ttattttgac tctcggtact tctctgcctg ttatccttaa tcctaagttt tttcaaacca
     9241 cctttggttt taatcttcct cgtatttcta atatcatttt gactatttgt cttattccct
     9301 tagccattct gattattttg gattggaaat tacgccctcc ccgccaaaag aaaggttttt
     9361 tgaatatcgt caaaaatatt cttcaatggc cccttatgcc cattgccact cttaccatgt
     9421 ctgttctccc cgggctccac tcccacaccc ggctattttt gggccgcaag cttgattaca
     9481 aaactaccaa caaaaaagct gttaaataag ctatcattta tcaatgacta aattcttatc
     9541 ccggcacaag tactgggttt ttgtcattat tctgtttatt tttcttcgac tgccgtctct
     9601 ttttgaacct tattggtatg gcgatgaagg tatttatctt acccttggtc aggctatcac
     9661 aaaaggggat attctttacc gtcaaatcca cgacaacaaa ccccccactc tttactatct
     9721 ggctgctatc agtcaaaccg tttttggttt tcgtcttctt ttagctctgt ttatggtccc
     9781 caccatttac tttttttacc gtctttgcct ttattttttt acttccaaaa ccgcccgaat
     9841 ttctctattg ttttttgtaa ttctgacctc gctgccaact cttgaaggca acattgctaa
     9901 tgctgaaatt tttatgctct tacccaccat tcttgct
//
LOCUS       DAOSHV010000012         9691 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_5980_length_9691_cov_5.016086, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000012 DAOSHV010000000
VERSION     DAOSHV010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 9691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9691
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5980_length_9691_cov_5.016086"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="PKZ29_00970"
     CDS             <1..429
                     /locus_tag="PKZ29_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37476.1"
                     /translation="IHLIILSTGGINSFLFFLIYFLLFSLAFQNPPTINLIYSLITIL
                     FYSFNLNSFQSLIQLLSLLLITPLSYFIALSRSSEIEAQKNLSRDQTDFLFWLSLRLK
                     KPLQDIISLSNQSQVRNIAKDLLKDAHKLTDSISSQDNDA"
     gene            419..1138
                     /locus_tag="PKZ29_00975"
     CDS             419..1138
                     /locus_tag="PKZ29_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37477.1"
                     /translation="MTLRIFLALSLLSTLFPPTVLAQRFSSPSYLINWGNFNLTSGSK
                     TSPNYRLTDTVGQNAPGYSEGDSLAVKAGFQYIYDTFDYLNFTIDKLLIDFGTLEPGL
                     EVTDTNLLTVTTPSGRGYQITAHEDHTLWQNSNVFIPDTTCDNNNCDANHAAIWTQNN
                     KYGFGFNAQGDGSLSYFPAPNLFRPFANLDYNQEPAVIASESNPVRNHQTLITYKVIV
                     SPDQSAGHYQTYITYTLTPNY"
     gene            1141..1989
                     /locus_tag="PKZ29_00980"
     CDS             1141..1989
                     /locus_tag="PKZ29_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37478.1"
                     /translation="MPQFNLGLKPAQIEITLIPGKSYTQAFEVTNNSNDSVVLTTSVS
                     AWQPLDNLGHIRHQTIDQLPFNFSLVNSDLRLGQEFILAPHQSRQLVLKIDNPEIRNI
                     DHYFSFFLSQQPSLLQNGQNLAKIGANILISTSTESVLAPLKIDFINLSPRLKDTFLG
                     LNITGQISNPSTRFTHLQGKITLKKNNEIFWERDIYPYTALSQNSRQIFCVTEDKHPE
                     DCHVPAPLWPGKYQLEIRQDPSTPHPDYTSTFFVFPYLLSFLILLVFLFLYLIIKNYQ
                     TRSSKA"
     gene            2105..3526
                     /locus_tag="PKZ29_00985"
     CDS             2105..3526
                     /locus_tag="PKZ29_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37479.1"
                     /translation="MKKYSLNQILIFSLFLGTLSLILPHQAASIPSKNVKDVLSSSQF
                     SYYGGVASGNAEGSSILTINTTDFPSRTSNNLFVGDTISVGIGGKQSLYTIRDIGATN
                     QILINPALTADAATIGGSIIATRSAIHTVSFEPQSSAVGGAWQVLIKAPSGPVEHASD
                     GIPDQGGFDAHYLNNAAVTCPWGATADVGTTIAGGEPGVPDGNYYHVISCNMTGSNPV
                     GTGETGTIIIGNTTNMLINPSSTHTDANLEGHANVFNFILRRTDTSNNVIEETPGRIA
                     IVESVRVTATIDPTLSFYIDNVGVTDVSSTACGVGTTLSNGAAFTTGDRVVFGSLELG
                     VHNQLAQRLSCVTNAPGGYVVTAYEAGVMKNINTDTTIPDTTCNGTGCTPTTATAWDG
                     VSSTLSEFGYTVTSLGATTAFSSPNFKPFGIGYANAQPIMLNTKTPLTTESANVCYRI
                     TATTTQEAGDYEAKVIYTATATF"
     gene            3529..4518
                     /locus_tag="PKZ29_00990"
     CDS             3529..4518
                     /locus_tag="PKZ29_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37480.1"
                     /translation="MKKSARLIILSVLVILITFFSKSVLAQQSSLGLSAIPPRLEITD
                     ANPGDVLTKEIKVRNESRTEKVITVSVEDFIVVDDLGTPVRIETESQNDNRWAAANWI
                     QVSPTQVKLKPGETKALVLTLLVPQDATPGGHYAMVLYNHNADTTITQTGAAIETKVV
                     SLIYVTVPGDITENAQVKEFSTKKFFEYGPVDFKTIITNLSDIHIAPAGSINIYNTFG
                     LRSHQLKLSNTNIFPYTSREFDNTLNRKFMFGYYRAKLNAVYGPSGKPIDATLSFWVI
                     PYKIVILVLIILIPLIFFIRQRKKNPPTTQNNPQIEALEQELEALKKKYQDRS"
     gene            4519..5235
                     /locus_tag="PKZ29_00995"
     CDS             4519..5235
                     /locus_tag="PKZ29_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37481.1"
                     /translation="MITLNKAVSVRASIPGTFVTIFGYTSPSSRVELSSPKAFAVTYS
                     DQSGYFLFDQVLLPKNPQELCLLSFDDANRSNNPVCFPEPPNNSKTDIGPILLSPTIS
                     LDLQNNFASGQSIPNSRIKIFFHEQTNQKISLIKTAHARSLPILEINTDSRGNYSINL
                     ADNSQSAYRLFSTTTFLENNSPKSNTLFYGHIYFQWFWLVLFIVFLTGFLVFFINRHR
                     SRRRHFLPSVIYNKDIAIYV"
     gene            5228..5638
                     /locus_tag="PKZ29_01000"
     CDS             5228..5638
                     /locus_tag="PKZ29_01000"
                     /inference="COORDINATES: protein motif:HMM:NF014428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOG37482.1"
                     /translation="MSKKILLIILGLFLILILLFFIGRQVILSSRASTTTNQLPTREN
                     SYIFASPIQAQANGQEKIRINVFLLDSQGLGVGQKQVTLSSNPSLNIEAIQPQTDDFG
                     KAIFNLSSFLPGKYQISASISDFTLKQQISVLFL"
     gene            complement(5647..6066)
                     /locus_tag="PKZ29_01005"
     CDS             complement(5647..6066)
                     /locus_tag="PKZ29_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOG37483.1"
                     /translation="MENKKLYLNLGIGLLVTLLLLFLATKVVPSVLVTLTKAAPASKV
                     SVGNSYILGGKTLAQADGKDKCIVNVFVLDKDSKGVKGIAVSLLGAPEGEVESVSGVD
                     GKATFELSSKMEGQFPVSAAIAGTPMAGGVKLTFRNE"
     gene            6707..7144
                     /locus_tag="PKZ29_01010"
     CDS             6707..7144
                     /locus_tag="PKZ29_01010"
                     /inference="COORDINATES: protein motif:HMM:NF014898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HOG37484.1"
                     /translation="MAISILPQHLNSYSLWLQKRQYQSNTIRNYLQDLKTFLTYSNFQ
                     ISSEIIKNYIQEITGKNNSKRHLASLASFCQFLLNQHITHSNLFRKGQRTIRHAQNED
                     VNSLLIQYQEQLKKENKSNLTIKNYLNDIHQYFDWLNQNESQE"
     gene            7128..>9357
                     /locus_tag="PKZ29_01015"
     CDS             7128..>9357
                     /locus_tag="PKZ29_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37485.1"
                     /translation="MNLKNNFSLSTLKKYFDFLKVKQSSNATLQRKLSSLSSFADFLS
                     KRGLIDSQDYSQLNLTISLAKPHRKRFNYPYVFNRYFILGSLIALFLGLAYSFYQQSI
                     LKTKQQLAYSTANQPVRPGRIMSFQGRLTDSAGNPITSSTAINFKFFNSAVGGTQLYN
                     SAIGNSQAVTPDSNGIFSVVIGKSHGNEIPQDVFSENPEVWLQITTGDEVMEPRQQIA
                     TVAYALNSETLQGLPPSASGEKNTVLVVDNQGNINLGESSPAIKSSSGTFSLEGESIL
                     IKATNSSHGGNIKLLTEGTAPTDGGFVDFSNPNISSGNLLNSQVTNTHRDYNFISFQN
                     YNPGTSQLATRFSVDANGNGMFSNNLLVGGTLTTTTLKATNAQDTTMISNLNADLLDG
                     KHASDFVGTGDTGNFLTDITAGVGITVSGSGTTRQVALTNTGVATGSYGGGGYFPTFT
                     VDAQGRLTAAGQIPIPQGTVYTASNGITLVGADFQINPGFSNTWTAVQNFNNNFTVGS
                     TAKINNLNADLLDGYDSTYFLNVGQTGALPYVNDVTNTTLTRSGAGPYTLALNLANAN
                     TWTATQTFQNNFAVGSPALINNLNADLLDGKHADDFLQVGGTGYDNYRYWTASANGVN
                     RQVGSTSTFNFVGANGVGLTLSGNTLTINGTTYAAGIALSLTPANVFNLRYDNSLAVN
                     GSNQLGLNLANSNRWTALQRFTAGIGVTGNSTFTNNLSVGQTLTTTTLKATNAQDTTM
                     ISN"
     assembly_gap    9358..9457
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2718 a         2289 c         1369 g         3215 t
ORIGIN      
        1 attcatctca tcattctttc taccggaggc attaattcct ttcttttttt tctcatctac
       61 ttcctacttt tttccctcgc ttttcaaaac ccacctacaa tcaaccttat ctactcttta
      121 atcactattc tcttttacag ttttaatctc aactcttttc agtctcttat tcaactactt
      181 tccctattac taattacacc cctcagttat tttattgctc tctcccgcag tagcgaaatc
      241 gaagctcaaa agaaccttag tcgcgaccaa actgattttt tattttggct gtcactgcgt
      301 ctcaaaaaac ctctacaaga catcatttct ctatctaatc aaagtcaagt tcgcaatatt
      361 gccaaagatc tcttaaaaga tgctcacaaa ttaactgata gtatcagtag tcaagacaat
      421 gacgcttaga atttttcttg ctctatcact tctgtcgact ttatttccac caacagtttt
      481 agctcaacgt ttttcttctc cttcatatct cattaattgg ggtaatttta atctaacttc
      541 cggtagtaaa acttctccta attatcgtct caccgatacc gtcggccaaa atgctcccgg
      601 atactccgaa ggtgatagtt tagctgttaa agccggtttc caatacattt acgatacttt
      661 tgactatctc aatttcacca tcgacaaact tttaatcgat tttggtactt tggaacccgg
      721 tctagaggtc accgatacta atttattaac cgtcactacc ccttcaggtc gaggttatca
      781 aatcactgct catgaagacc atactctctg gcaaaactct aatgttttta ttcctgatac
      841 tacctgtgat aacaataatt gtgacgctaa tcacgctgcc atttggactc aaaacaacaa
      901 atatggtttt ggttttaatg ctcaaggcga cggctctctg tcctattttc ccgccccaaa
      961 tctatttcgc ccttttgcca atcttgatta taatcaagaa cctgccgtca ttgcctcaga
     1021 aagtaatcct gtgcgcaatc atcaaacttt aatcacctat aaagtcatcg tctcccccga
     1081 ccaaagtgcc ggtcactatc aaacttatat tacttatact ttaactccca actactaata
     1141 atgccccaat tcaatcttgg tcttaaaccc gctcaaatcg aaattacctt aatcccgggc
     1201 aaatcctata cccaagcttt tgaagttacc aataattcca acgattcagt tgttctgact
     1261 acctctgtat ctgcttggca accactggat aacctgggtc acatccgaca tcaaactatt
     1321 gaccaacttc ctttcaattt tagtctagtc aatagtgatc ttcgcctcgg tcaagaattt
     1381 atcttagctc ctcatcaaag ccgtcaatta gttcttaaaa ttgacaatcc cgaaattcgc
     1441 aatattgacc attatttcag tttttttctt tcccaacaac cttccctgct ccaaaatggt
     1501 caaaacttgg ccaaaatcgg tgctaatatt ttaatttcta cctctactga gtctgttttg
     1561 gctcctttaa agattgattt tattaatctt tctcctcgac tcaaagatac ctttctaggt
     1621 cttaacatta ctggtcaaat ttccaatccc tcaacccgtt ttacccacct tcaaggtaaa
     1681 atcactctca aaaaaaataa cgaaatcttt tgggaaagag atatttatcc ttatactgcc
     1741 ctgtctcaaa actctcgtca aatcttttgt gtcaccgaag acaaacatcc tgaagattgt
     1801 catgttcccg cccctctttg gcctggaaaa tatcaactcg aaatcaggca agatccttca
     1861 actccccacc ctgactatac tagtactttt tttgttttcc cttatctgtt atcttttctt
     1921 attctattag tatttttatt tttatattta atcattaaaa actatcaaac tagatctagc
     1981 aaggcttaaa caactctgct aaaggaattg ttttatccct ctgattaggt aaataaaaca
     2041 aagagttgac aataaacttt ataaacctta aactgattct ataaaatagt aatcctgtaa
     2101 actcgtgaaa aaatattctc taaaccaaat cttaattttt tccctattct taggaacttt
     2161 aagtttaatt ctacctcatc aggccgcttc cattccctct aaaaatgtca aggatgtcct
     2221 atcttcgtct caattctcct actatggagg ggttgcttca ggcaatgccg agggtagttc
     2281 tattttaact attaatacta ctgatttccc ttctcgcacc agtaacaacc tttttgttgg
     2341 tgatactatt tcagtgggta ttggtggtaa acaaagtctt tacactatta gagatattgg
     2401 cgccactaat caaattttga ttaatccagc tttaactgct gatgccgcta ctattggtgg
     2461 tagtatcatt gctacccgct ccgccattca tactgttagc tttgaacctc aaagtagtgc
     2521 tgtaggtggt gcttggcaag ttcttatcaa agccccttcc ggtcctgttg aacatgccag
     2581 tgacggtatt cccgatcaag gtggtttcga tgcacattat ctcaacaatg cagccgttac
     2641 ttgtccttgg ggtgctactg ctgacgtagg taccactata gctggtggtg aaccaggagt
     2701 ccctgatggt aattactacc atgttatttc ctgtaatatg actggcagta atcctgtcgg
     2761 tactggtgaa accggtacta ttattatcgg taacaccacc aatatgttga ttaatcccag
     2821 ctccactcat actgatgcca atcttgaagg tcatgccaat gtttttaatt ttatcctccg
     2881 acgaaccgac acttctaata atgttattga ggaaacccct ggtagaattg ccattgtcga
     2941 atccgttcga gttactgcca ccatcgatcc tacccttagt ttctatattg ataatgtcgg
     3001 tgtcactgac gtttcctcta ctgcctgtgg agttggtact actcttagta atggcgccgc
     3061 ctttactact ggcgacagag tcgtttttgg ttctctagaa ctaggagtcc acaaccagct
     3121 cgcccaaaga ttatcttgtg ttactaatgc tcctggaggt tatgtagtta cagcttatga
     3181 ggctggagtc atgaagaata tcaacactga cactaccatt cctgatacca cttgtaatgg
     3241 caccggttgt actcctacta ccgccacagc ttgggatgga gttagttcca ctttatcaga
     3301 atttggttac actgttacca gtttaggtgc cactactgcc ttttcttcac ctaactttaa
     3361 accttttggc attggttatg ctaatgctca acccatcatg cttaacacca aaaccccgct
     3421 caccactgag tcggctaacg tttgttaccg gattactgcc actaccactc aggaagccgg
     3481 tgactacgaa gctaaagtta tatataccgc tacagctact ttctaaatat gaaaaaatct
     3541 gcccgcctca tcattctaag cgttttagta attttaatca cctttttttc caaatcagtt
     3601 ttagctcaac agagctcctt gggtctcagt gctattccgc ctcgtctaga aattactgat
     3661 gctaatcctg gagatgtgct tactaaagaa attaaagttc gcaacgaatc acgcactgaa
     3721 aaagtaatca ctgtttcagt tgaagacttt attgttgttg acgacctggg tactccggta
     3781 cgtattgaaa ctgaaagtca aaatgacaat cgttgggctg cggctaattg gattcaagta
     3841 tcccccactc aagtaaaact taaaccaggt gaaactaaag ctttagtttt aactctctta
     3901 gtacctcaag atgctacccc cggtggccat tatgccatgg ttctttacaa tcacaatgct
     3961 gacaccacta ttactcaaac tggtgctgcc attgaaacta aagtcgtcag tctaatttat
     4021 gtcactgttc ccggagatat tactgaaaac gctcaagtca aagaattttc tactaaaaaa
     4081 ttttttgaat atggtcctgt tgattttaaa accattatta ccaatctttc agatattcat
     4141 attgctcctg ctggttctat taacatttac aacaccttcg gtcttcgtag tcatcaatta
     4201 aaactcagta acactaatat cttcccttac actagtcgcg aatttgacaa tactctcaat
     4261 cgtaagttta tgtttggcta ctatcgagct aaactcaacg ctgtctacgg tcctagtggt
     4321 aaaccaattg atgccactct ttcattctgg gttattccct acaaaattgt catcctggtc
     4381 ttgatcatcc tgattcccct catcttcttt attcgtcaaa gaaagaaaaa tccccctaca
     4441 acccaaaaca atcctcagat tgaggctttg gaacaggagt tagaagctct caagaaaaaa
     4501 taccaggatc gatcttaatt gattactcta aacaaagctg tctctgttcg tgctagtatt
     4561 cccggtactt ttgtcaccat ttttggttat actagcccct cctccagagt cgaactttcc
     4621 agtcccaaag ctttcgccgt cacttatagt gaccaatctg gttattttct ctttgatcaa
     4681 gttcttcttc ctaaaaatcc tcaagaactt tgccttctct cttttgatga cgccaatcgc
     4741 agtaataatc cggtctgttt tcccgaacct cctaataatt ctaaaactga tattggtcca
     4801 attctcctct ccccaaccat ttccctggat ttacaaaata atttcgcctc cggacaatcc
     4861 atccctaata gtcgtattaa aattttcttt catgaacaaa ctaatcaaaa aatttcttta
     4921 attaaaactg ctcatgctag atctctacct attttagaaa tcaataccga tagtagaggt
     4981 aactatagca ttaatctcgc tgataattct caatccgctt atagactttt cagtactact
     5041 acttttttgg aaaacaattc acctaaatct aatactcttt tttatggaca catctacttt
     5101 cagtggtttt ggctcgtgct atttatagtt ttcttaactg gattcttggt cttttttata
     5161 aaccgccacc gttctcgtcg tcgtcacttt ctcccttcag tcatttataa taaagacatc
     5221 gctatatatg tctaaaaaaa ttcttttaat catcttggga ttatttttaa tattgattct
     5281 tcttttcttt ataggtcgac aagtaatttt aagcagccgc gctagtacta ccactaatca
     5341 acttcccact agagaaaact cctatatctt tgcttcccct atccaggctc aagccaatgg
     5401 tcaagaaaaa atccgtatta atgttttttt acttgactcc caaggtttag gtgtcggtca
     5461 gaaacaagtg actctctcct caaacccctc acttaacatt gaagctatcc aacctcaaac
     5521 cgatgatttt ggtaaagcta tcttcaacct ttctagtttt ttacctggaa aatatcaaat
     5581 cagtgcttcc atttcagatt tcaccctcaa acaacaaatc tctgttcttt ttctttaaac
     5641 ctttttttat tcgttcctaa aagttaattt cacccctcca gccattggtg tccccgctat
     5701 ggctgctgac accgggaact gaccctccat tttagaactt aattcaaagg tagccttgcc
     5761 atcaacccct gaaactgact ctacttcacc ttccggtgct cctaataaac taactgctat
     5821 tcccttaacc cctttactgt ccttgtctaa tacaaataca tttactatac atttatcttt
     5881 accatctgcc tgagccaaag tcttccctcc taaaatatag gaattaccta cagacacctt
     5941 tgatgccgga gctgcttttg ttaatgtcac taaaaccgaa ggtactactt tcgtagctaa
     6001 aaacaaaagt aataaggtta ccagtaatcc tatacccaaa ttcaggtaca actttttgtt
     6061 ttccatagcc aatgatagca aatattcctc ctgacttcat attttagcac tccgtaatca
     6121 tgagtgataa aggttttatt attatcattt taccctattt ttaaaacaaa aatacaatca
     6181 ataaactttt atttttattt tttaattctt ctaaaacttc tcgtaatttt tttattttat
     6241 ttaaagattc tttctatatc agcccctgct tactatatca attaagatct cttctttagt
     6301 catttttttt atctctaaat ttcattaaat ttaatcaaaa tcactctatt tttaataaat
     6361 ttatttaatt taatatatta attaatatca taatatctta accgcacttt ttccgaaaaa
     6421 ataatttttt aattcccaaa aaagttatta atacatatct tttgatacat atttatagta
     6481 cctaaaacca atatgaaaat atatgtgaaa tttattaaat acttcaaata aaaccaaatg
     6541 atattaaata atatatagca aactatatca actaagttgt aaatctggct attgacaccc
     6601 ttttcctagt agtagatatt aataattaat atatataaac atagctgata aacagataaa
     6661 catgtataaa aaatataatt tcaaagctaa aagcaattta taacttatgg ctatctctat
     6721 cctgccccaa cacctcaatt cttactctct ttggctccag aaacgtcaat atcaatctaa
     6781 caccattcgt aactacctcc aggatctcaa aactttttta acctattcta actttcagat
     6841 atcttctgaa attatcaaaa actacatcca ggaaattacc ggtaaaaata attccaaaag
     6901 acatttggca tctctcgcct ctttctgcca attcctcctc aatcaacata tcacccacag
     6961 caatctcttt cgcaagggtc aacgtacaat ccgacatgct caaaacgaag acgttaattc
     7021 tctcttgata cagtatcaag aacaacttaa gaaagagaac aaatcaaatc taaccattaa
     7081 aaactatctc aatgacattc accagtattt tgactggtta aatcaaaatg aatctcaaga
     7141 ataatttttc tctctctact ctcaaaaaat acttcgactt tctcaaagtc aaacaatctt
     7201 ctaatgccac ccttcaaaga aaactttcct ctctttcttc ttttgctgat tttctaagta
     7261 aacgtggcct aattgattct caagattaca gtcaacttaa tctcacaatt agcttggcta
     7321 aacctcatcg taaacgtttc aattacccct atgttttcaa tcgatatttt attttaggta
     7381 gtctcatcgc tctttttcta ggtttagctt acagttttta tcaacaaagt atccttaaaa
     7441 ctaaacaaca attggcttat tctaccgcca atcaaccagt cagaccaggt cgaattatgt
     7501 catttcaagg tcgtctcact gattcagccg gcaaccctat taccagttcc actgctatta
     7561 attttaaatt tttcaatagc gctgttggtg gaactcaact atacaattct gctattggca
     7621 acagtcaggc agttactcct gactccaatg gtatttttag tgtcgttatc ggcaaaagtc
     7681 atggtaatga gattcctcaa gatgtttttt ctgaaaatcc tgaagtttgg ttgcagatca
     7741 ccactggaga tgaagtcatg gaaccccgtc aacaaattgc cactgttgcc tatgctctta
     7801 attctgaaac ccttcaaggt ttacctcctt ccgcttccgg agaaaaaaat acagtgctgg
     7861 ttgtcgacaa ccaaggcaat atcaatctag gtgaaagttc ccctgctatt aaatctagtt
     7921 ccggtacctt ttcccttgaa ggtgaaagta ttctcattaa agccactaat agtagtcatg
     7981 gtggcaatat taaattgctc actgaaggta ctgccccaac tgatggcggt tttgttgatt
     8041 tcagtaaccc caatattagt tctggtaact tacttaattc tcaagtcact aacactcatc
     8101 gagattataa ctttatttct tttcaaaatt ataatcctgg tacttctcaa ctagctactc
     8161 gtttttcagt cgacgctaac ggtaatggta tgttttctaa caacttatta gttggtggta
     8221 ctcttaccac caccactctt aaagctacta atgctcaaga taccaccatg atcagtaatc
     8281 ttaatgctga tctccttgac ggtaaacatg cttctgactt tgtcggtact ggcgacactg
     8341 gtaatttctt aaccgatatc actgctggtg tcggtatcac tgtcagtggt tccggtacca
     8401 ctcgtcaagt cgccttaacc aatactggag tcgctactgg tagttatggt ggtggcggtt
     8461 acttccccac ctttaccgtt gatgctcaag gtcgtttaac tgccgccggt cagattccta
     8521 ttcctcaagg tactgtctac accgccagta acggtatcac tctagtgggc gctgatttcc
     8581 aaattaaccc tggcttctcc aatacctgga cagccgtcca aaactttaat aataatttca
     8641 ctgttggttc tactgccaaa attaacaacc ttaatgccga tctcttggat ggttatgatt
     8701 ctacttactt tcttaatgtc ggtcagactg gtgctctacc ctatgttaat gatgttacca
     8761 ataccacctt aaccagaagt ggtgctggcc cctacacttt agctcttaac ctcgccaatg
     8821 ctaacacttg gacagccacc caaacctttc aaaataattt tgcagttggt tctcccgctt
     8881 taatcaacaa tcttaatgct gatctccttg acggtaaaca tgctgatgac ttcctccaag
     8941 ttggtggtac cggttacgac aactatcgtt actggactgc ttctgccaat ggggttaatc
     9001 gccaagtcgg ctccacctct acttttaact ttgttggtgc taatggtgtt ggtctcaccc
     9061 tctctggtaa cactctcact atcaatggca ccacctatgc cgccggtatc gctttaagtc
     9121 tcactcccgc taatgttttc aatcttcgtt acgacaactc tttagctgtc aatggcagta
     9181 atcaactcgg tcttaatcta gctaattcta atcgttggac agctcttcaa cgtttcactg
     9241 ctggtatcgg tgtcaccggt aactctacct tcaccaacaa tctctctgtc ggtcaaacct
     9301 taaccaccac cactcttaaa gctactaatg ctcaagatac caccatgatc agtaatcnnn
     9361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntaa ctgccgccgg tcagattcct
     9481 attcctcaag gtactgtcta caccgccagt aatggggtta cccttgttgg caacgatttc
     9541 caaattgatc atgatttttc caatacctgg acaggggtcc aaacttttca aaataatttt
     9601 aatgttggtt cctcggcctt aatctctaat cttaacgctc aatacctcaa tggtcatcct
     9661 gacactgact ttttacacat tggtgggact g
//
LOCUS       DAOSHV010000013         9000 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_6589_length_9000_cov_5.251873, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000013 DAOSHV010000000
VERSION     DAOSHV010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 9000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9000
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6589_length_9000_cov_5.251873"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..731)
                     /locus_tag="PKZ29_01020"
     CDS             complement(36..731)
                     /locus_tag="PKZ29_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37486.1"
                     /translation="MTTDPLKIAIKGSTQDHLPIEDVVDGIVIMKDGSCASIMQISSV
                     NFDLLSEKEQSALVAAYGGILNSLNFPIQIVIKSSTKDVGIYLKRLMEAEEKQTNKLL
                     KERIKSYRKFIDETVKKNDVLSKTFYVVVKFSSLELGIKSGGKQTITSLFGKRATSLP
                     YPKGEILEKAKASLEPKRDHLIRLFSRLGLEIIPLTNRQSVELFYKIYNEEVATNQKM
                     ETTNYQNPIVTTK"
     gene            complement(748..1326)
                     /locus_tag="PKZ29_01025"
     CDS             complement(748..1326)
                     /locus_tag="PKZ29_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOG37487.1"
                     /translation="MSAVKDTDWAKEKFIREKFSLRRKNKGLMDEGEKNTVEEEAVEA
                     LKAMEVTKEPAATPAKTEETKVEETKTVDWRDEKANLNLKTEKLGATGKAVFGSIPMP
                     DRPDTANVIVGMATTTQGKIIDGVIVEIQDSHGNPARVIKTNLLGQFKISTPLTDGRY
                     LIIAEKDGYTFDRVNVDLTGKIVDPIRIIANN"
     gene            complement(1351..1701)
                     /locus_tag="PKZ29_01030"
     CDS             complement(1351..1701)
                     /locus_tag="PKZ29_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37488.1"
                     /translation="MEQHPIPQQISSYEFKLVGEMTLKQFLKAAGGILLAVLINSTKI
                     IVIVKWPLMLFFGGLGLLLAFVPFEDRPLETWITAFIRSIYAPTVFLYKKKNDGSWLE
                     FKTTQSETETDEPE"
     gene            1751..2641
                     /locus_tag="PKZ29_01035"
     CDS             1751..2641
                     /locus_tag="PKZ29_01035"
                     /inference="COORDINATES: protein motif:HMM:NF014759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HOG37489.1"
                     /translation="MSKLLLIDGHAIIHRAYHSIPDLDFRGQPVNALYGFYSMLISAL
                     TQLEPKYLIVCLDSPGPTFRQSEFVGYRAKRKPADRSLVSQLPLLASSLADAGVATFS
                     FGGYEADDLIATIAHRAENKPFIDQTIIITGDKDLMQLVSSKTSLLMPLRGLSETKLF
                     QPQDVFDKLGVYPHQVVDLKALMGDMSDNYPGVAGIGPKAAADLLLTHHDLDSIYLHL
                     DQIKPSLRQKLTQDKETAYLSQKLALLIDDIPLKFKFKDSLWNQNLFHQLKQMLKNYN
                     FKSLISRLDQFYQPRQSSLF"
     gene            2643..3794
                     /locus_tag="PKZ29_01040"
     CDS             2643..3794
                     /locus_tag="PKZ29_01040"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOG37490.1"
                     /translation="MSLKIALVQDYIKEFGGAESVLENLSDIFPQAPIFTSIYHPASL
                     GPHQTRLRQKWDHRVHQSFFKYIPLAHKIISPLRLISPLAFRSFDFSDFDVIITSATG
                     AYFPNSLNKKSAKLICYCHTPPRYLYGLPTARRFTQNPVIKFLVDILNHFLRLHDFRY
                     SQNVDQYLANSRTTAARIHKFYRRQAIVINPPVSLPESKNTKYQILNTKKYFLAGGRL
                     AQAKRFDIAIQACNQLGLKLKIFGRDFSGVLDQLKQISGPTIEFVGEVDQTVKSQLYR
                     QAQAFIFSADQEDFGIVPVESMSYGCPVIAYKSGGVTETVIDGKTGVFFDQLTPQSCA
                     AAITKFQKLNLKPSDCISRAAEFSTQKFIQKIKKTILPYESKPQKSKAK"
     gene            complement(3748..4014)
                     /locus_tag="PKZ29_01045"
     CDS             complement(3748..4014)
                     /locus_tag="PKZ29_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37491.1"
                     /translation="MKKFLKIGWQIIKWFLFLFIWLIYAYSTLDRSTGEPIPSGYSFI
                     VLPILISILVKKQVFNKKLWGWDLFWKVVVILLLIFGVLIHTVK"
     gene            complement(4024..4989)
                     /gene="trxB"
                     /locus_tag="PKZ29_01050"
     CDS             complement(4024..4989)
                     /gene="trxB"
                     /locus_tag="PKZ29_01050"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014021151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOG37492.1"
                     /translation="MKETKQILDTLIIGSGPAGLTAAIYNSRANLKTIVIAGNQPGGQ
                     LTLTTLVDNYPGFPTGVGGLKLMMDMQQQVKNLGVEIKQGIVKEIKKISGKINGFEVG
                     LESGEEIETKSVIVATGAAVKWLGLPGEKELIGRGVSGCATCDGMFFRGKTVAVVGGG
                     DTACEEADFLAKICQKVYLIHRRDELKASVTEQKKVLNNPKIEIWWNSEVKEFKGEEK
                     LEEVRVVNNVTGEEKMLDLDGLFVAIGSQPATRFLEGMVDLSPTGQVLVGGDENWVTM
                     TNVPGIFAGGDCVNNRHKQAIVAAGEGCRAALDAENWIINSGNKN"
     gene            5054..5974
                     /locus_tag="PKZ29_01055"
     CDS             5054..5974
                     /locus_tag="PKZ29_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37493.1"
                     /translation="MIKKTKITLFLIFKIIQDKKRFLFWFFIRFLSALLPLLTIYLYS
                     QVIGQVEKQVVFSSIFFLILLIFIVRLLDNFVRLKSIEGLNQCISNISFDIHNFFSRD
                     IRAGNKEERNQTIQAIRNFADASTITLTLFRQPGVDSIVSLIIIPAILYFVDVRVLIL
                     FISYILVYLIVDYYTTQRYVKLKDIQNTKIENYYSKFQESNDVELEQKAYTRHFARIC
                     NWSFIEWFSIQNVAVFFYSLILAYSVISVYSGNWQISNVILVMGYAGSLQAYLNSFSE
                     IKDNLADMTVAVDHLAKNKHISTIDLDDLI"
     gene            6015..6869
                     /gene="mutM"
                     /locus_tag="PKZ29_01060"
     CDS             6015..6869
                     /gene="mutM"
                     /locus_tag="PKZ29_01060"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HOG37494.1"
                     /translation="MPELPEVEVVRQFLDTNIVGKTITHLEVLNPKSFIGNPDQLKHQ
                     PIIGTSRLGKQLSLHLKNGLILLFHLKMTGQIIFGASVWGHPTPKEDKSHLPNKSTRL
                     VFTFSDQTKMYFNDQRKFGWVKLFTPDQLTQFQQSIGLDLLDPKFTPAYFYRQLQSSR
                     RPVKSVLLDQNKFAGVGNIYDNDALFLSQINPQTPANQLTFSQAKLLRSHLIKLIKES
                     IAHGGSTAKDNKYVRPDGSFGQHQYHFRVYQRQGEPCSVCRTPIVRISLGGRGTFYCP
                     HCQPFLLK"
     gene            6873..7331
                     /locus_tag="PKZ29_01065"
     CDS             6873..7331
                     /locus_tag="PKZ29_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37495.1"
                     /translation="MTFVKAAYAQCPVCIITVGGGMLLAQKLGIDDFLVSLWISGLNT
                     AISFWMATKIKHRIFGSPIILSILMYLLTLSYFIFTRQTGSTNNQILGIDKIILGQTL
                     GLLIWGLGIFIDRRSRVLNGGKILFKFQKVIFPVGTLVIFTLIFKLIFKL"
     gene            7344..7865
                     /locus_tag="PKZ29_01070"
     CDS             7344..7865
                     /locus_tag="PKZ29_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37496.1"
                     /translation="MVTNQDIQKFIDKTKDLEKGHQMDLSSGEDLSLAIMNLISIEEH
                     MFFTSQKLGQIKYLDILDTAREMRKELMKKIVKEGQGEVWCTSKHLLSASMRLMEVGT
                     KYLTKGDKHEANSYFKKSFDLYNLFWGLNLGLVDKSSAKPKFKPADQPLPKKDLLGSI
                     GSVINKILDCCRE"
     gene            7869..8513
                     /locus_tag="PKZ29_01075"
     CDS             7869..8513
                     /locus_tag="PKZ29_01075"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOG37497.1"
                     /translation="MSEIKDIGNLSLPDSIDYTVEFPYAVLPALIEAKKKAFKDQATE
                     CYNRNFWKDYANHFDGHRGDRASLVVIDINNLKKTNDAPVESGGGHLAGDKIIKKTAD
                     FLMANFTRKNDRIIRFGGDEFIILCNHINGSNSKTDSTPSSDSDKPIDKFDHWLNSIF
                     SPETLSALGLDFAYGAAHFNPQEDHDIEDTLRRADKKMYQKKMEMKSNLPANIG"
     gene            complement(8530..8973)
                     /locus_tag="PKZ29_01080"
     CDS             complement(8530..8973)
                     /locus_tag="PKZ29_01080"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HOG37498.1"
                     /translation="MKNKKIYRNQTNELVAGVCSGLADYFEVDATLVRIVFVVLGIGG
                     GSGVLIYLVLWFVIPRENEVNMGKEEQLENVGKEIEGKVKIAAKEIKKEVKTEKRSGD
                     FLGMVLVILGGVLLVEKIAPRFIDWDYFWPMILIVLGAYLIFRKR"
BASE COUNT         2406 a         2305 c         1299 g         2990 t
ORIGIN      
        1 ttgttttttt atctttttta ccaaataagt tcatattatt ttgttgttac tattgggttt
       61 tgataattag ttgtttccat tttttggttg gtagccacct cttcgttata tattttataa
      121 aataattcta ctgattgccg gttggttagg ggaattattt ccaaccctaa tcgagaaaat
      181 aatcttatca aatgatctct ttttggctcc aaactcgctt tagctttctc caaaatttcc
      241 cccttcgggt agggtaggct ggtagctctt ttgccaaaca gactggttat agtttgtttt
      301 cctcctgatt taattcccaa ttccaaggaa gaaaatttaa ccaccacata aaaagttttt
      361 gacaacacat catttttttt caccgtttca tcaataaatt tgcgataact tttaattctc
      421 tctttcaata atttattggt ttgtttttcc tccgcttcca tcagcctttt taaataaatc
      481 cccacatcct tagtgctgga tttaattaca atttgaatcg gaaaatttag tgaatttaaa
      541 attcctccgt aagctgccac cagcgccgat tgttcttttt ccgacaacaa atcaaaattt
      601 actgatgata tctgcataat cgaggcgcac gacccatcct tcattattac gatcccatcc
      661 accacatcct caattggcaa gtggtcctga gtactgcctt ttatcgctat tttcaaagga
      721 tcggttgtca tattaattaa tcataaacta attgttggca ataatcctaa ttggatcaac
      781 tatttttcca gtcaaatcaa cattaaccct gtcaaaagta tagccgtctt tttccgcaat
      841 aattaaatat ctgccatcag tcagtggagt tgaaatctta aactgaccca acaagttggt
      901 tttaatcact ctggccgggt taccgtggct gtcttgaatt tctacaatca ctccgtcaat
      961 aatttttccc tgagttgtgg tggccattcc tacaattaca ttagccgtat ccggcctatc
     1021 cggcatcgga attgatccaa acaccgcctt gcctgttgct cccaattttt ctgtctttaa
     1081 atttaaattt gctttttcat ctctccaatc aaccgtcttt gtctcttcta cttttgtttc
     1141 ctctgttttt gctggcgttg ccgctggttc ttttgttacc tccatcgctt ttaatgcctc
     1201 caccgcctcc tcttctactg tatttttctc cccttcatcc attagtccct tattttttct
     1261 tctcaaacta aatttttctc taataaattt ttctttagcc caatccgtat ccttaactgc
     1321 cgacacaaat aagtttttct tttccacccc ttattccggt tcatctgttt cagtttccga
     1381 ctgagtcgtc ttaaattcca accagctgcc atcatttttt ttcttataca aaaaaaccgt
     1441 cggtgcataa attgatctga taaaagccgt aatccaagtt tccagcggtc tatcttcaaa
     1501 tggcacaaat gccaacaaca accctaaacc tccaaaaaac aacatcaatg gccactttac
     1561 aatcacaatt attttggttg aatttatcaa caccgccagc aaaattcctc ccgccgcttt
     1621 caaaaattgt ttcaaggtca tttctcccac cagcttaaat tcgtaggagc tgatttgctg
     1681 tggaattggg tgttgttcca tgattaaatt ctaccagttc ttggtttaaa cgtctttggt
     1741 aaactacctt atgtccaaac ttttgcttat tgatggtcac gccattattc atcgggccta
     1801 tcactctatt cctgacctag actttcgtgg ccagccagtg aatgctcttt atggttttta
     1861 ttccatgctt atttctgccc taacccagtt ggaacctaaa tatttaattg tctgtctcga
     1921 ttctcctggt cccactttcc gccaatccga atttgtcggc taccgcgcca aacgtaagcc
     1981 cgccgaccgt tccttagtct cccaactgcc tcttttggct tcatctttgg ccgatgccgg
     2041 agtcgctacc ttctcttttg gcggctacga agccgatgat cttattgcca ccattgccca
     2101 ccgggctgaa aacaaacctt ttattgacca aaccatcatc attaccggcg acaaagatct
     2161 tatgcagcta gtcagttcca aaacctctct tttgatgcct ctccgcggtt tatctgaaac
     2221 taaattattt caaccccagg atgtttttga caaacttggt gtttatcccc atcaggttgt
     2281 cgacctcaaa gccttaatgg gggatatgtc tgacaactat cccggcgttg ccggcatcgg
     2341 tcccaaagct gctgccgacc ttcttttaac ccaccacgat cttgattcca tttaccttca
     2401 tcttgaccag atcaaacctt ctcttaggca aaagctgacc caagataaag aaaccgccta
     2461 tctttcccaa aagttagctc tccttattga cgacattcct ctcaagttca agttcaaaga
     2521 ttctctttgg aaccaaaact tatttcatca gctcaagcaa atgttaaaaa attataattt
     2581 taaatccttg atttcccggc tggatcaatt ttatcagccc cgccaatcaa gtctttttta
     2641 atatgtctct taagattgct ctggttcagg attacatcaa agaatttggt ggcgctgaaa
     2701 gtgttttaga aaatctttct gacattttcc cccaagctcc aatttttact tccatttatc
     2761 accccgcctc ccttggtcct caccagaccc gtctccgtca aaaatgggac catcgtgttc
     2821 accagagttt ttttaaatat attcctttag cccacaaaat tatcagcccc ttgcgcctta
     2881 tttccccact tgctttcaga tcttttgatt tttccgactt tgatgtcatt atcacctctg
     2941 ccaccggcgc ctattttccc aattcattaa acaaaaaatc tgccaaactt atctgctatt
     3001 gtcacacccc tcctcgctat ctttatggtt tgcctaccgc ccgtcgtttt actcaaaatc
     3061 cggttattaa atttttagtt gatattctca accatttcct ccggctccat gatttccgtt
     3121 attcccaaaa tgttgatcaa taccttgcca attctcgcac caccgccgcc cgcatccaca
     3181 aattttaccg ccgccaggcc atcgtcatta accctcctgt cagtcttccg gagtcaaaaa
     3241 atactaaata ccaaatacta aatactaaaa aatattttct tgctggcggt cgtctggccc
     3301 aagccaaacg gtttgatatt gccatccaag cctgcaacca gctaggtctc aaactcaaaa
     3361 tctttggtcg tgatttttcc ggtgttttag accaactcaa acaaatttcc ggtcccacta
     3421 ttgaatttgt cggcgaagtt gaccaaaccg tcaaatctca actttaccgt caggcccagg
     3481 ctttcatctt ttctgccgac caagaagatt ttggcattgt ccccgtcgag tccatgagct
     3541 atggctgtcc cgtgattgcc tacaaaagcg ggggagttac cgaaactgtt attgacggca
     3601 aaaccggcgt tttctttgac caacttactc cccaatcctg tgccgctgct attactaaat
     3661 ttcaaaaact aaatcttaaa ccctccgact gtatttcccg tgccgccgaa ttttccaccc
     3721 aaaagttcat ccaaaaaatc aaaaaaacta ttttaccgta tgaatcaaaa ccccaaaaat
     3781 caaaagcaaa ataaccacca ccttccaaaa taagtcccaa ccccataatt ttttattaaa
     3841 aacttgtttt ttcaccagaa tcgaaatcaa aattggtagg acaataaaag aatatccgct
     3901 tggtattggt tcacctgtgc ttctgtccaa agttgaataa gcgtaaatca gccaaataaa
     3961 cagaaacaaa aaccatttaa taatttgcca tccaatcttt aaaaattttt tcatttttag
     4021 atattaattt ttattgccac tatttattat ccagttttcc gcatccaacg ctgcccgaca
     4081 accttctccg gcagccacaa tcgcctgttt atggcggtta ttgacgcaat ctccgccggc
     4141 aaagattccc ggcacatttg tcatcgtcac ccagttctca tctcctccca ccaatacttg
     4201 tcctgttggg cttaaatcta ccattccctc caaaaatcta gtcgccggct gactcccaat
     4261 ggccacaaac aaaccgtcca aatccaacat tttctcctct ccggtaacat tatttaccac
     4321 cctaacttct tccaattttt cctctccctt aaattcctta acttcgctat tccaccagat
     4381 ctctatcttg ggattattta gcaccttctt ttgttctgtt accgatgctt ttagttcatc
     4441 tcttcggtga attaaataaa ctttttgaca tatttttgcc aaaaaatctg cctcttcaca
     4501 agccgtatct ccacccccga ctactgccac cgttttcccc ctaaaaaaca tcccatcaca
     4561 agtagcacaa ccgctgaccc ctcgcccgat taactctttt tctcccggca aacccaacca
     4621 cttaaccgcc gctcctgtgg ctacaatcac tgattttgtt tctatttctt ctccactttc
     4681 caaccctacc tcaaatccat taatttttcc cgatattttt tttatttcct ttactattcc
     4741 ttgctttatt tccactccca aattttttac ctgctgctgc atatccatca tcagcttcaa
     4801 tcctcccact cccgtcggaa acccggggta attatctacc aacgtcgtca aagtcaactg
     4861 tcctcccggc tgattgccgg cgattactat tgtttttaga ttagcccttg aattataaat
     4921 tgccgccgtc aaccccgccg gtcccgaacc aattatcaaa gtgtccaata tttgtttagt
     4981 ttccttcatg acttcatttt accacccctt tagctttact tttttagtct ttactctaat
     5041 attttattag gccatgatca aaaaaactaa aattacgtta tttcttattt tcaaaatcat
     5101 tcaggacaaa aaacggtttc tcttttggtt ttttatccgc tttttatccg cccttttacc
     5161 ccttttgact atttatcttt attcacaagt aatcggtcaa gttgaaaaac aggtagtttt
     5221 tagctccatt ttttttctta ttcttttgat ttttattgtt cggttactgg ataattttgt
     5281 ccgtctcaag tctattgaag gcttaaacca gtgtattagt aatattagct ttgatatcca
     5341 caactttttc tcccgcgaca ttcgcgccgg caacaaagaa gaacgcaatc aaaccattca
     5401 agctatccgc aattttgccg atgccagcac cattactctt accctctttc gtcagcccgg
     5461 agttgattcc atcgtttctc tgataattat tccggcaatt ctttactttg ttgacgttag
     5521 ggttttaatt ttattcatta gctatatttt ggtttattta attgtcgatt attacaccac
     5581 ccaacgctat gtcaaactca aagatatcca aaataccaaa atcgaaaatt attacagtaa
     5641 attccaagaa tcaaacgacg ttgaactgga acaaaaagcc tatacccgtc atttcgcccg
     5701 catttgcaat tggagtttta ttgaatggtt ttccatccaa aatgtcgccg tcttttttta
     5761 ctcccttatc ttagcttaca gtgttatttc ggtttattct ggcaactggc agatttccaa
     5821 tgttattttg gttatgggtt atgccggttc tctccaggct tatctcaact ctttttccga
     5881 aatcaaagac aatcttgccg acatgacggt tgcagttgat catctggcca aaaataaaca
     5941 tatttctacc atcgacctcg acgatcttat ttaggctggc tttataaact ttgcaaactt
     6001 gataaactag gtctatgcca gaactccccg aagttgaagt tgttcgtcaa tttttggaca
     6061 caaatattgt cggcaagacc atcactcatc ttgaagtact caaccccaaa tcattcattg
     6121 gcaatcctga ccagctcaaa catcagccta ttatcggcac ttccagatta ggcaaacagc
     6181 tttctctcca cctcaaaaac ggcttaattt tactgtttca tctcaaaatg accggccaaa
     6241 ttatttttgg cgcctccgtt tggggtcatc ccacccccaa agaagacaaa tcccatcttc
     6301 ccaacaaatc cactcgcctc gtttttactt tttctgatca aaccaaaatg tatttcaatg
     6361 accagcgcaa atttggttgg gttaaattat ttactcctga tcaattgacc caattccaac
     6421 agtctattgg tcttgacctt ttagacccca aattcacccc tgcttacttt taccgtcaac
     6481 tccaatcctc ccgtcgtcct gtcaaatcag ttcttcttga ccaaaacaaa tttgccggtg
     6541 ttggcaacat ctacgacaac gatgctttgt ttttaagcca aattaacccc caaactcctg
     6601 ccaaccagct cactttttcc caagctaaac ttcttcgttc tcatctcatt aaacttatta
     6661 aagaatctat tgcccacggc ggttctactg ccaaagacaa taaatatgtc cgccccgacg
     6721 gtagttttgg tcagcatcaa tatcactttc gggtttatca gcgtcagggt gaaccctgtt
     6781 cagtctgccg cacccccatt gtcagaatta gtcttggggg tcggggcact ttttattgtc
     6841 cgcactgcca accctttttg ctaaaataga ctatgacttt tgtcaaagct gcttacgccc
     6901 aatgtcccgt ctgtattatc accgttggtg gcggtatgct tttggcccaa aaacttggca
     6961 tcgacgattt tttagtttct ttatggattt ccggtcttaa tactgccatt tccttttgga
     7021 tggccaccaa aatcaagcac cggatttttg gcagccccat tattctctca attttaatgt
     7081 atctcctaac cttatcttat tttattttta cccgccaaac cggtagcacc aacaaccaaa
     7141 tcttgggcat cgacaaaatc attctcggcc aaaccctagg tttgcttatt tggggtttgg
     7201 gtatttttat tgaccgccgc agccgcgttc tcaacggtgg caaaatcctt tttaagttcc
     7261 aaaaagttat tttccctgtt ggcactctgg ttatctttac tttaattttt aaattaattt
     7321 ttaaattata aattttcact cctatggtta ccaaccaaga cattcaaaaa tttattgaca
     7381 aaaccaaaga tctggaaaaa ggccatcaaa tggatctttc ctccggcgaa gatttatccc
     7441 tggctattat gaatctcatc agtatcgaag aacatatgtt ttttacctcc caaaaacttg
     7501 gccaaatcaa atatcttgat attcttgata ccgcccggga aatgcgaaaa gaattgatga
     7561 aaaaaattgt caaagaaggt cagggggagg tttggtgcac cagcaaacat cttttgtcag
     7621 cctccatgcg tctcatggaa gttggcacca aataccttac caaaggcgac aaacacgaag
     7681 ccaattctta ttttaaaaaa tcatttgatc tttacaatct tttctggggc cttaacttag
     7741 gtttagtcga taaatcttct gccaagccca aatttaagcc cgctgatcaa cccttgccca
     7801 aaaaagacct tcttggctct attggtagtg ttatcaacaa aattcttgat tgctgccgcg
     7861 aataatctat gagtgaaatc aaagatatcg gcaatttatc tttacccgat tctattgact
     7921 ataccgttga gtttccttat gctgtcctcc ccgctctcat cgaggccaaa aaaaaagcct
     7981 tcaaagatca ggctaccgaa tgttataatc gcaatttttg gaaagattat gcgaatcatt
     8041 ttgatggtca ccgtggagac agagccagtt tagttgttat tgatattaat aatctcaaaa
     8101 aaaccaacga tgctcccgtt gaatctggtg gtggccatct agccggtgac aaaattatca
     8161 aaaaaactgc cgattttctt atggccaatt ttactcgcaa aaacgaccgt attatcagat
     8221 ttggcggtga tgaatttatc attctttgca accatattaa tggttctaat tccaaaactg
     8281 attccacccc atcttccgac tctgataagc cgatcgataa attcgatcat tggcttaact
     8341 ctattttcag cccagaaacc ctgagcgcct taggtttaga ttttgcctat ggtgcagctc
     8401 attttaatcc ccaagaagat catgatatcg aagacaccct ccgccgcgcc gacaaaaaaa
     8461 tgtatcagaa aaaaatggaa atgaaatcca accttcccgc aaacattggt taattttttc
     8521 aaccagtgat tatcttttcc taaaaataag ataagctccc aaaactatca gaatcatcgg
     8581 ccaaaaataa tcccagtcaa taaatcttgg cgcgattttt tctaccagca acactcctcc
     8641 cagtatcacc aataccattc ccaaaaaatc acccgatctt ttttccgttt taacctcttt
     8701 ttttatttct ttggcggcta tttttacctt tccttctatc tctttgccca cattttccaa
     8761 ttgttcttct ttgcccatat taacctcatt ttctctggga ataacaaacc acaacaccaa
     8821 ataaatcaaa actccactcc caccgccaat ccccaacact acaaaaacaa ttcttaccaa
     8881 agttgcgtct acctcaaaat aatccgccag tccgctgcac accccggcaa ctaactcatt
     8941 tgtttgattg cggtatattt ttttattttt cacaaattta ttttatcagt tttgtaacga
//
LOCUS       DAOSHV010000014         8890 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_6697_length_8890_cov_6.925863, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000014 DAOSHV010000000
VERSION     DAOSHV010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 8890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8890
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6697_length_8890_cov_6.925863"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            136..528
                     /locus_tag="PKZ29_01085"
     CDS             136..528
                     /locus_tag="PKZ29_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37499.1"
                     /translation="MIFLLEQCLNYTTPRDVREMWEKAGHTSIHGMPSPNEVELLVSA
                     FVILTENDKNSWCTSYPWDKIVDQVEKFPRAKGLRIREEAKDWLDFLSDTKLVHQQTR
                     DGKTEICLSQGLVEKAVKAVIDMLPHKS"
     gene            637..942
                     /locus_tag="PKZ29_01090"
     CDS             637..942
                     /locus_tag="PKZ29_01090"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HOG37500.1"
                     /translation="MMNFNQRVYKVVEKIPKGKVMTYKQVAEKLNSKAYRVVGQALKN
                     NPNPPIIPCHRVIRSDGSVGGFFGDTEGKMAEEKIKLLESEEVKIVNGKIADDGYIL"
     gene            946..2088
                     /locus_tag="PKZ29_01095"
     CDS             946..2088
                     /locus_tag="PKZ29_01095"
                     /inference="COORDINATES: protein motif:HMM:NF015755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HOG37501.1"
                     /translation="MQKRKIAWWWIAAGVLGLIMAILAAVYVNYNKMVVKPNKKELET
                     SVAPTATPTPDPLAPYSILLLGYGGANHEGGFLTDSIVVAKIRPRDEEVDLISVPRDL
                     WVKIPINDEETVDKKINEAFSIGMNDKKYPNKKVEFTGKAGGGELAKKVISEVLGFKI
                     DYFASIGFDGFIKIIDNLGGIDIKVVRTFDDPKYPIEANIKDTCGKTDEEVTALTATM
                     SGEKLEDQFPCRYENLHFNRGWQHMNGATALKFARSRHSPTNGGDFNRAERQKLVINA
                     VKDRVINIDFIPKIIPTFNILSRYIMTDIDFATMNELVAKLPQLSEYKIVSIALTDKN
                     VLTNGVATTGQFILLPKSGEDNWEEIHQFIENKGVATSSAAIETQN"
     gene            2128..2547
                     /locus_tag="PKZ29_01100"
     CDS             2128..2547
                     /locus_tag="PKZ29_01100"
                     /inference="COORDINATES: protein motif:HMM:TIGR04272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37502.1"
                     /translation="MRNFNNDRGFKSFDNRGFGDKKQKRFSRNRSGFGRDRSNQDRQM
                     YEAVCDKCGKVCQLPFMPTGEKPVYCSNCFEKTGDREGNKRRFDNRGSHNFGQENIHA
                     EIEMINQKLDKILAALKPQTPAKEVKKTKKKLTSDES"
     gene            complement(2527..3885)
                     /locus_tag="PKZ29_01105"
     CDS             complement(2527..3885)
                     /locus_tag="PKZ29_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012192216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HOG37503.1"
                     /translation="MNIDTSIFKSYDIRGIYPDQADEKLAESIGRGFAKYLNFPSQVI
                     VARDGRISGPSMKQSLIDGLTKVGVSVIDIDQASTDMFYYACKTRDLPGISVTASHNP
                     KEYTGFKMVKRIPELLSGDAGIKEIKDWILADNLPADSATPGQVEKLNIMAEFIDYVL
                     SLVDVSQLKNFKVVADPANGMVGPILEKLQSKLPTISFVPMYWDVDGNFPNHGGDPLL
                     EPNRVDIQARVPVENADLGIMFDPDGDRFFVIDNQGRFISGDFLTAILSRYFLRQYPG
                     CSIVYDIRASKAVRETISALGGKALANRVGHTHIKARMKSENAFFGGEISGHYYFKDF
                     FLCDSGITTFIYLLDFLSHSDQPLTRLVDELNSKYFISGEINNKVANVPAVMSKIESI
                     YGPQAISPVEKMDGLTFEMGDWRFNLRSSNTEPLLRLNLEADSQELMVQKRDEVLALI
                     TG"
     gene            3978..4334
                     /locus_tag="PKZ29_01110"
     CDS             3978..4334
                     /locus_tag="PKZ29_01110"
                     /inference="COORDINATES: protein motif:HMM:NF013734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37504.1"
                     /translation="MDQINYDDFAKVEFKIGEIKEAVEVEGSEKLLRLRVDMGEDDLR
                     TVFSGIKKWYEPKDLINKKTVFVTNIKPRKMMGELSEAMIFAASEEITNDKGEKEETA
                     VLLFPENDLPAGSKVF"
     gene            4339..6090
                     /gene="argS"
                     /locus_tag="PKZ29_01115"
     CDS             4339..6090
                     /gene="argS"
                     /locus_tag="PKZ29_01115"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOG37505.1"
                     /translation="MKIADQLKIIILDSVEAKVDPKEVKIEHPNDNQFGDYSTNIALI
                     MAKKLKQNPRKLAEEMQGQINDYVLKNKIDLIEKVEMAGAGFINFYLNPSYLIEEAEI
                     INYDIEFRRKLAQNFEGKTMVIDYSAPNIAKPFGIGHLRSTNIGQSIYNIYKILGWRC
                     IGDNHIGDWGTQYGKLMAAIVHAMNNDSRFIIGESKLNLDKITIDDLENLYVKFHKEA
                     EKDENLAEEGRQWFAKLEQGDKEAKKIWQKCVDISLREFERVYQMLGVKIDFVHGESF
                     YQDMISGVTKEIEGKKITKKSQGAVIVELKGMPPAMLEKSNGTTTYFARDMAAIKYRK
                     EKWNPDLIIYEVGSDQQLHFRQVFAAAELMGWFNQDQLVHVAHGLIRWPTGKFSTRKG
                     DTIHLSTVMEKAMAEAKKIADKSEVDKKLTATEKEEMIKTVAIGAVKFSDLVSDPKKD
                     IIFDWDKVMSLEGDSGPYLQYTYARCKSVLNKTKIKEQKNISRVSGKINNEEMSLVKE
                     FYKFEEKIIEAAERFSPSVVAEYILGIARKYNEFYAKNWIINQKEEVFRVFLTRTAAS
                     IIETGLDLLGIETVEKM"
     gene            6106..6603
                     /locus_tag="PKZ29_01120"
     CDS             6106..6603
                     /locus_tag="PKZ29_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37506.1"
                     /translation="MKENIKIWICPPDQRLVSPVREPVTDEEKINFQKLNDSGAVKLL
                     SDMRDNQVVYQRKSESGEGKDGKTIKYEPAVVNVGRDFRSVGILFDGVTSSRYEDGSF
                     VRFDLAEDDEFLSLSYDVSGIRKIDKRIRTADLADEMFKILLDRQAEIDIRKAKNLGS
                     GTTTS"
     gene            complement(6596..>8890)
                     /gene="lon"
                     /locus_tag="PKZ29_01125"
     CDS             complement(6596..>8890)
                     /gene="lon"
                     /locus_tag="PKZ29_01125"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOG37507.1"
                     /translation="LFPSVETSLFFGRKESMNSLLHAYDNTNKLVIITTQKDGRIENP
                     NFNDLYNVGVLAKIEHILQTDGNLHAIVKGVSRVNIVNLVKTDPFFLAEYVDLPIIPE
                     PVSEVQQSAETLLSQLKKAFALGRQFDLPAMMQLSTGVSSSDLADQVSFSINAKLAEK
                     QGLLETLIVSQRLRAATEYLIQEMKIAELERSIEEKTQEKFNTGIKKNVLEERKRQIE
                     KELKRLGQSSGGEFDELESKLKKLNLPKDVRKKLLKELDRLIEMGPMAPESSYIKTYL
                     ETVTELPWGKYNHSKISLTKASQILEADHYGIKDVKERILEYLAVIQLKNKSDIKKRQ
                     DNSGTANILCFIGPPGVGKTSLGKSIAKALGRGFVRASLGGIRDEAEIRGHRRTYVGA
                     MPGRIIKGLKEAKSMNPVFMLDEVDKIGSDYRGDPSSALLETLDPEQNYAFSDHYIDF
                     PVDLSKVFFILTGNDLSTIPGPLRDRLEIINFSGYTLDEKFHIAKKYLIKKELSANGL
                     TVKNIVPLQDSTIRYIIEKYTREAGVRGLDRTISSLFRKVAKKIVTKNISQVDLNNQA
                     TLRKFLGPEKFSTTLKGKKNEIGVSTGLAWTSVGGDILFVEVNIMPGKGQLILTGKLG
                     SVMKESCQAALSFVRSQSKNFGINHDFHKIDIHIHVPEGATPKDGPSAGGAITTALVS
                     ALKNVAIPKDIGMTGEITLRGNILEIGGLKEKSIAAHRAGLKTIFIPKENQKSLVDVP
                     KEVKKDIKFIPVSHYTEIYKHIFS"
BASE COUNT         2990 a         1456 c         2066 g         2378 t
ORIGIN      
        1 gctcaaagtg ttataattta cgtcctaagt tcatatagta attaaatttt gagttaatag
       61 caactctaaa atatttttgc taatagcact ttgaaattaa ccaggctgga gcttggttaa
      121 aataaatgga aaagggtgat ttttttgttg gaacagtgtc tcaactacac taccccacgc
      181 gatgtcaggg agatgtggga gaaagccggt catacctcaa tccatggaat gccgagtccc
      241 aacgaggtgg agctgttggt atctgctttt gtcatactga ccgaaaacga caaaaactcg
      301 tggtgcactt cgtatccgtg ggacaagatt gttgaccagg tggaaaagtt tcccagggca
      361 aagggtctga gaatcaggga ggaggccaag gactggctgg atttcttgtc tgacaccaag
      421 ttggtgcacc agcaaaccag agatggaaaa accgaaattt gtctcagcca agggttggtg
      481 gagaaggctg ttaaagcagt gatagacatg ctcccccaca agagttaaac ccaaatcctt
      541 tacccccaag tcggtcaaga cgatttacac gaaagtgtgg attgatttta attgataagg
      601 gggttttatt ttgggggaaa ttttgggtat gatttaataa tgaattttaa tcaaagggtg
      661 tataaagtag tcgaaaaaat tcctaagggt aaagtaatga cttacaaaca agtggctgag
      721 aaattgaatt caaaagctta tcgggtggta ggacaagcgc taaaaaacaa tccaaaccca
      781 ccgataatcc cctgccacag ggtgatcaga agtgacggaa gtgtgggtgg attttttgga
      841 gacactgagg gaaaaatggc agaagaaaaa ataaaattat tggaaagtga ggaagtaaaa
      901 atagtaaatg gcaaaattgc cgatgatggg tatatacttt gacttatgca aaaaagaaaa
      961 atagcgtggt ggtggatagc agcaggagta ttaggactaa taatggcaat tttggcggcg
     1021 gtttatgtga actacaacaa aatggtggtt aaaccaaata aaaaggaatt ggaaacaagt
     1081 gtagcgccga cagcaacgcc gacaccggat ccgctggcac catattcaat tttactgctg
     1141 ggttatggcg gagctaatca tgaaggtgga tttttgactg actcaattgt ggtggcaaaa
     1201 atcagaccaa gagacgagga agttgatttg atatcggtgc caagagattt gtgggtaaaa
     1261 ataccaataa atgatgaaga aacggtggat aaaaaaatca atgaggcttt tagtatcggg
     1321 atgaatgata aaaaatatcc aaacaaaaaa gtagaattta caggcaaggc cggaggtgga
     1381 gagctggcca aaaaagtaat tagtgaagta ttgggattta aaattgatta ttttgcttca
     1441 atcggttttg atggatttat aaaaataatt gataatttgg gcggaattga tataaaggtt
     1501 gtcagaactt ttgatgatcc caaatacccg atagaagcta atattaaaga tacttgtggc
     1561 aaaaccgatg aagaagttac agcgctaacg gcaaccatgt cgggagaaaa attggaggat
     1621 caatttccct gccgctatga aaatctgcat tttaacagag gttggcaaca tatgaatgga
     1681 gctacagccc ttaaatttgc caggtcgaga cactcaccga caaatggggg tgattttaac
     1741 cgggcagaaa gacaaaaatt agtgataaat gccgtcaaag acagagtcat taatatcgat
     1801 tttattccca aaataatacc gacttttaat atcttatccc gttacattat gacggatatt
     1861 gattttgcta ccatgaacga attagtagca aaattaccac aactatcaga atacaagata
     1921 gtatcgatag ccctgacaga taaaaatgtt ttaactaatg gagtggcgac aactggtcaa
     1981 ttcatattgc tgccaaaatc aggagaagat aattgggaag aaattcatca atttattgaa
     2041 aataaagggg tagcaacatc atcggcggca attgaaactc aaaactgata caatacttag
     2101 tctgatttaa taagtttaaa agataaaatg agaaatttta ataatgatcg cggatttaaa
     2161 agctttgata atcgcggttt tggtgacaaa aagcaaaaaa ggttcagtcg aaaccgaagc
     2221 ggttttggca gagaccgaag caatcaagac aggcaaatgt atgaagcagt ctgtgataaa
     2281 tgcggtaagg tttgtcaatt gccgtttatg ccgacaggtg agaaaccagt ttattgcagc
     2341 aactgttttg aaaaaaccgg cgaccgcgaa ggtaataaaa gaaggtttga caacagagga
     2401 agccataatt tcggtcaaga aaatattcac gcggaaatag agatgatcaa ccaaaagctg
     2461 gataaaatat tggcagcgct taaacctcaa actccggcaa aggaagtcaa aaagactaag
     2521 aaaaaactaa ccagtgatga gagctaaaac ttcatcacgt ttttggacca ttaattcctg
     2581 agagtcagct tctagattaa gacgaagcag gggttcggtg ttggaggaac gaaggttaaa
     2641 acgccaatcc cccatttcaa aggttaaacc atccattttt tccaccggag aaattgcctg
     2701 gggtccgtag attgattcaa tttttgacat aaccgcaggg acattggcaa ctttgttgtt
     2761 aatctcaccg gaaataaaat atttggaatt gagctcatca accagccgag tcaggggctg
     2821 gtcggaatga gacaaaaaat ccaaaaggta aataaaggtg gtaataccgc tatcgcagag
     2881 gaaaaaatcc ttaaaataat agtgaccaga gatttctcca ccaaaaaagg cattttccga
     2941 cttcattcta gctttgatat gagtgtgacc aacacggttg gctagggctt tgccgccaag
     3001 agcagaaatg gtttctctaa cagcttttga agcccgaata tcgtagacaa tactgcagcc
     3061 aggatattgc cggagaaaat atcgagacag aatagcggtt aaaaaatcgc cggaaataaa
     3121 cctgccttga ttgtcgataa caaaaaatct gtccccatcc ggatcaaaca taattcctag
     3181 gtcagcattt tcgaccggaa ctcgggcttg gatatcaacc cggtttggtt ctaaaagcgg
     3241 atcgccgcca tgattgggga aattaccatc aacatcccaa tacatgggca caaaactgat
     3301 ggtcggtaac ttcgattgca atttttctaa aatcggcccc accataccgt tggcaggatc
     3361 agccacaact ttaaaatttt taagctgaga aacgtcaact aggcttaaaa cataatcaat
     3421 aaattcagcc ataatattaa gtttttcgac ttgaccgggg gtagcagaat cggcaggtag
     3481 attgtcggca agaatccaat ctttgatttc tttgataccg gcatctccgg acaatagttc
     3541 gggaattctt ttgaccatct taaagccggt gtattctttg gggttatgac tggcagtcac
     3601 ggaaattccg ggtaaatctc tggttttgca ggcataataa aacatgtcgg tagaagcctg
     3661 gtcgatatcg atgacactaa ctccgacttt ggtaagtccg tcaataaggg attgcttcat
     3721 agaaggtccg gaaatgcgac catcacgggc gactatgact tgtgagggaa aatttaaata
     3781 tttagcaaaa ccgcgaccga tactttcagc taatttttcg tcggcctgat cgggataaat
     3841 gccgcggatg tcgtaagatt taaaaatgga ggtgtcaata ttcattaatg agttaaacca
     3901 ataagaatta gccaaagaat atcattttga taattagttg tctaattttt gggcagaaat
     3961 ttttgttaaa attagccatg gatcaaatta actatgatga ttttgctaaa gttgaattta
     4021 aaattggaga aataaaagag gcggttgaag tagaaggatc ggaaaaattg ttgagactaa
     4081 gggtggatat gggagaggat gatttaagga cagtattttc cggaatcaaa aaatggtatg
     4141 agccaaaaga tttgattaat aaaaaaactg tttttgtgac taatatcaaa cccaggaaga
     4201 tgatgggaga gctgtcggag gcaatgatat ttgcggcttc ggaagaaata acaaacgaca
     4261 aaggagaaaa ggaagagacg gcggtattat tattcccgga aaacgatttg ccggcggggt
     4321 caaaagtatt ttagacatat gaaaatagca gaccaactaa aaataattat tttggattcg
     4381 gtagaagcaa aagtagatcc aaaggaggtt aaaatagagc acccaaatga taatcagttt
     4441 ggtgattatt cgaccaatat tgccctgata atggcaaaaa aactcaagca aaatccaaga
     4501 aaactggcgg aggaaatgca aggtcagatt aatgattatg ttttgaaaaa taaaatagat
     4561 ttgatagaaa aagtggagat ggcgggagcg ggatttatta atttttacct taatccaagt
     4621 tacttgattg aagaggcaga aataattaat tatgatattg aatttagaag gaagctggca
     4681 caaaattttg aaggtaagac aatggtgata gattattcgg cgccgaatat tgccaagccg
     4741 tttggtatcg gtcacttaag gtcgactaat attggtcagt caatttataa catttacaaa
     4801 atattgggat ggagatgtat tggagacaat catatcggcg attggggaac tcaatatggc
     4861 aaattgatgg cagcaattgt ccatgccatg aacaatgact caagatttat cattggtgaa
     4921 tcgaaattaa atttggacaa aataactatt gacgacttgg aaaatttata tgttaagttt
     4981 cacaaagagg cagagaaaga cgaaaactta gcagaagaag ggcggcagtg gtttgccaag
     5041 ctggaacaag gtgataaaga agccaaaaaa atctggcaaa aatgtgttga tatttctttg
     5101 agggaatttg aaagggtata tcaaatgttg ggagtaaaga ttgattttgt tcacggagaa
     5161 tctttttatc aggatatgat ttcgggggta actaaagaga ttgagggtaa aaaaattacc
     5221 aaaaaaagtc agggggcagt tatagttgaa ctaaaaggaa tgccaccggc aatgttggaa
     5281 aaatcaaatg gaacaacaac ttattttgcc agagatatgg cagcaattaa atatagaaaa
     5341 gagaagtgga accctgattt gattatttat gaagttgggt cagaccaaca actgcatttt
     5401 cgccaggtat ttgcggcggc agaactgatg ggctggttta accaagatca gttggtgcat
     5461 gtggctcatg gattaatcag gtggccgaca ggaaaatttt ccactagaaa gggtgacact
     5521 atccatttgt cgacagtaat ggaaaaagcg atggcagagg caaaaaaaat tgctgacaaa
     5581 agtgaagtgg ataaaaaatt aacggccaca gaaaaagaag aaatgattaa gacggtagca
     5641 atcggagccg tcaaatttag cgacctggtc agcgatccaa aaaaagatat tatttttgac
     5701 tgggacaagg tgatgagtct ggagggagat tcggggcctt atttgcaata tacttatgcc
     5761 cggtgcaaaa gtgtgctaaa taaaaccaaa ataaaagaac aaaaaaatat cagccgagtt
     5821 tcgggaaaaa taaataatga agaaatgtct ttggtaaaag agttttataa gtttgaagaa
     5881 aaaataatag aggcagcaga gagattttca ccctcggtgg tggcggaata tatattggga
     5941 atagccagaa aatataatga attttatgcc aaaaattgga taatcaatca gaaagaagag
     6001 gtgtttcgag tgtttttgac acgcacggcg gcaagtataa ttgaaaccgg attggatttg
     6061 ttggggatag agacggtaga aaaaatgtag tgctataatg acccaatgaa ggaaaatatt
     6121 aaaatttgga tatgtccccc agatcaaaga ttggtatcgc cagtaagaga accagtaacc
     6181 gatgaagaaa aaattaattt tcaaaaattg aatgatagcg gggcggtaaa acttttgtct
     6241 gacatgagag acaatcaggt ggtatatcaa agaaaaagcg agtcaggaga aggtaaagac
     6301 ggtaaaacga ttaagtatga accggcggtg gttaatgtgg ggagagattt taggtcggtg
     6361 ggaattttgt ttgacggggt gactagcagc agatatgaag acggcagctt tgttagattt
     6421 gatttggcgg aagacgatga atttttatcc ttatcctatg acgttagcgg tattagaaaa
     6481 attgacaaaa gaattagaac ggcagatctg gcagatgaaa tgtttaagat tttgcttgat
     6541 cggcaggctg aaatagatat aagaaaggca aaaaatcttg gcagtggaac gacgactagc
     6601 taaaaatatg tttatagatt tcggtgtaat gagatacagg aataaattta atatcttttt
     6661 tgacttcctt ggggacatcg actaatgatt tttggttttc tttgggaata aaaatagttt
     6721 tcagtcctgc ccgatgagca gcaatcgatt tttccttgag accgccaatt tccaagatat
     6781 tgccccggag ggtgatttcg ccggtcatac cgatatcttt gggaatagcc acgtttttga
     6841 gcgcagacac cagggcggtg gtaatagcgc caccggcaga agggccgtct ttgggagtag
     6901 cgccctcggg aacatggatg tggatatcaa ttttgtgaaa atcgtgatta atgccaaaat
     6961 ttttggattg actgcggacg aaagacagag ccgcctgaca agattctttc atcaccgaac
     7021 cgagcttacc ggtcaaaatt agctgacctt tgcccggcat aatattgacc tcaacaaaaa
     7081 gaatatcacc gccgacagaa gtccatgcca aaccagtaga aactccgatt tcatttttct
     7141 tgcccttgag agtggtagaa aatttttccg gacccaaaaa ttttcttaaa gttgcctgat
     7201 tatttaagtc aacctgactg atatttttgg taacaatttt tttggcaacc tttctgaata
     7261 aagaagaaat agttctatcc aaacctctga cgccggcctc ccgggtatat ttctcaataa
     7321 tatatctgat ggtggaatct tgaagaggaa caatattttt gacagttaaa ccgttggcag
     7381 agagttcttt tttgataaga tattttttgg caatatgaaa tttttcatca agagtgtaac
     7441 cggaaaaatt gataatttcc agacgatcgc gcaaaggtcc tggaatagta gaaagatcgt
     7501 tgccggtaag aataaaaaag actttggaaa gatcaaccgg aaaatcaatg taatgatcag
     7561 aaaaggcata attttgttcc ggatctaaag tttcaagcaa agcagaagaa ggatcgccgc
     7621 ggtaatcaga gccaattttg tcgacttcgt ccaacataaa aaccgggttc atagatttag
     7681 cttcttttaa gcccttaatg atccgaccag gcatagcccc aacataggtg cggcggtgac
     7741 cgcggatttc agcttcatcc cggataccgc caagggaggc gcggacaaat ccccgaccca
     7801 aagccttggc aatagattta cccaatgagg ttttgccaac accgggagga ccaataaaac
     7861 ataaaatgtt ggcagtgccg gaattgtcct gacgtttttt gatgtcggat ttatttttga
     7921 gttgaataac ggcgagatat tcgagaattc tttctttaac atctttgata ccgtagtggt
     7981 cagcctccaa aatttgagag gctttagtta ggctgatttt ggagtgatta tatttgcccc
     8041 aaggtagttc ggtaacagtt tctaaataag ttttgatata gcttgattcg ggcgccatcg
     8101 gccccatctc aatcagccgg tcaagttctt tgagtagttt tttgcggaca tctttgggga
     8161 gattgagttt ttttagtttt gattcaagtt cgtcaaattc accgccactg ctttgaccca
     8221 aacgcttcag ctccttttcg atttggcgtt tgcgttcttc taagacattt tttttgatgc
     8281 cggtgttaaa tttttcctga gttttttctt cgatagagcg ttctaattcg gcaattttca
     8341 tttcctgaat cagatattca gttgcagccc gaagacgctg actaacaata agagtttcta
     8401 acaaaccctg tttttcggct aatttagcgt tgatagaaaa actgacctgg tctgccagat
     8461 cactgctgga aactccggtg cttaactgca tcatggccgg cagatcaaat tggcgtccca
     8521 gggcaaaagc cttttttagc tgactgagaa gagtttcggc agactgttgg acttcggaaa
     8581 cgggctcggg aataatggga agatcgacgt attcggccaa aaagaagggg tcggttttga
     8641 ctaaattgac gatgtttacc cgggaaacgc ctttgacaat agcatgaaga ttaccatcag
     8701 tttgaaggat atgttcgatt ttggctaaaa caccgacatt ataaagatca ttaaaattgg
     8761 gattttcaat ccgaccgtct ttttgagtgg tgatgataac caatttatta gtattgtcgt
     8821 aagcatggag caaagaattc attgattctt tgcgaccaaa aaaaagcgaa gtttcgactg
     8881 aaggaaaaag
//
LOCUS       DAOSHV010000015         8404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_7227_length_8404_cov_5.054977, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000015 DAOSHV010000000
VERSION     DAOSHV010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 8404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8404
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7227_length_8404_cov_5.054977"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..156)
                     /locus_tag="PKZ29_01130"
     CDS             complement(<1..156)
                     /locus_tag="PKZ29_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOG37508.1"
                     /translation="MILIVGGAGYIGSTVNKELSKKDYKTVVFDNLSTGKKELVKWGE
                     FFEGDLGK"
     gene            complement(194..1306)
                     /gene="metK"
                     /locus_tag="PKZ29_01135"
     CDS             complement(194..1306)
                     /gene="metK"
                     /locus_tag="PKZ29_01135"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOG37509.1"
                     /translation="MNYPVFVSESVCAGHPDKICDQVSDTILDEALVRDKNARVAVET
                     LATFNRLIVAGEVNCRQGIDYETVARKVILGLGYDNEDFGFWHKSPVEILVHQQSSDI
                     AVGVDNDGAGDQGIMYGYACKETPELMPLPIMLSHNICRKMDELMNSDLPYLRPDGKA
                     EVSVVYERGRPKTVEKIVLAKPFEKDLSGDEVKKQLLKLAVLPLLDKFRLGEIAENKV
                     VFNGTGSWKFGGPTSDTGLTGRKIVVDTYGGMGRIGGGCFSGKDPTKVDRSGAYAARY
                     IAKNIVAAGLADRCEIQLAYAIGQRDPVVKSIETFGTARKSARTIEGFGWGLMDLSVR
                     GIIDKFDLKRPIYKKTAKYGHFGDNSYPWESLVESK"
     gene            complement(1463..2407)
                     /locus_tag="PKZ29_01140"
     CDS             complement(1463..2407)
                     /locus_tag="PKZ29_01140"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOG37510.1"
                     /translation="MFKKIINSKLIKLVISSILIYFAFKKVDVVSLARDLSRVEWWFL
                     VVNILISLGLMGMISWRWSLLLIKNPTFKDIILFFKSSLSAAFYGLFLPSSAVAGDAL
                     KWIIIDEKYPEISKPKLLGSILLDRFVGLTVFVVLGSIMIFLAGNKGVEIPMMIQLVF
                     AGLLLGCLTFYVTIIFFDIKKVFNFKWLNKFSGILELVDKKNLVQIGKSLGVSLMSDI
                     FWVLQMWFIGWYFGTGLSVVSILIFLPVISTILTLPISFAGFGAREQLFVYFFGKEAV
                     SIESVLLMSTFAGILGVINSLVGGLVTLTPDFKRSQKK"
     gene            2450..3679
                     /locus_tag="PKZ29_01145"
     CDS             2450..3679
                     /locus_tag="PKZ29_01145"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOG37511.1"
                     /translation="MVKVLLLSHIFPPAVDGGSRVVHQLGQYFASQNFDTIYLSSDCS
                     STDDFVKSNYRPSRQLTSQIKLPVYHRLRRPLKLINLFLPQKTYLHGLLSVFQKGPVF
                     RFFPFLKSIFTVLKFKPDYIVAGPLPTTIIWYAHIFKHLLKKLSRHQVKILINASFHV
                     SDPDFHQLPLIRSLRHSDYIWTLSQFETDYFIHHLHLPPKKIILAGNGVNSGFIKKTT
                     TPNRLKPNQPINLLFIGSISAHKGLDTLIDSFSYLISQKNSPHFRLIIAGQKTLYYPI
                     IKQKISSLPKPFSTKIKFIFDFPQSRLKYLIDNSYTLILPSRHESFGLVIIEAWARGK
                     PVITSNIATLSELVSRAKGGLTFKLNNHLDLSSQIIKLVSSPQTAFKLGQNGKKYVKN
                     NYLWTKVGQKICQKLLS"
     gene            3658..4122
                     /locus_tag="PKZ29_01150"
     CDS             3658..4122
                     /locus_tag="PKZ29_01150"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HOG37512.1"
                     /translation="MPKITIIIIILIFAAVIYFISRPKAKPQNFDNNVSAIIKGNPYY
                     LEVAKTFTSRQTGLSHRTDLCSNCGMLFVFDREGKYPFWMKDTKIPLDIIWLNSKFEV
                     VKIITATETDSLKSFTNDQPALYVIELNANEVFKLDLKVGDIIQLSNVTNES"
     gene            4112..5134
                     /locus_tag="PKZ29_01155"
     CDS             4112..5134
                     /locus_tag="PKZ29_01155"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOG37513.1"
                     /translation="MSPKAAPSLSYSLVIPNYNGSAFILDCLKSIFSAVKNCPHTPFE
                     IILVDNNSHDNSPLIFKNFFQKNKLPNLQSKVCYLTSNTGFAAAVNYGINRSNYSHIV
                     VVNNDIVLKSNWFKLVSDAIVKNTIPKISTFFGTVLTRDGQKFESQGLKFYPNGRCHN
                     IANGQKYFTSKLPQTPKIVWGASAALAVYPKKVIKSVDMFDPDFFAYEEDVDLCLRFY
                     KLGYQTIYVPQAISYHLGGATSKLMGNFRYRMDAKNWIYIIIKNYSSKEIKDYFLALS
                     IERLRNLSGLIKSTIRIYKFKSIFYLPFDIVRTYGEVIVNLSKMIQKRRQIQKMIKYG
                     HYKTTL"
     gene            5131..6267
                     /locus_tag="PKZ29_01160"
     CDS             5131..6267
                     /locus_tag="PKZ29_01160"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOG37514.1"
                     /translation="MIIGIDVSSVCYQTGVSNYTLNLVKNLVNLDKSNHYKLFYSSLR
                     MPLPESIKILGQKANVTIHHHRLPPTLLQFVWNRLRLFPIEIFIGKCDLFHTSDWTQP
                     PTVFAKTIATIHDLTPFLYPQWHQPKVVNAHRHKMYWAVKKCRHLICVSQNTQSDLLK
                     LFPQVDIKKTSVIYESAESKYSQFLKLPAPTQDKKKLVIKKQYGLENFILAQGTREPR
                     KNLDRLIDAFLKFKQKYPKTRTQLAITGKYGWGKDVTVKDFSIKILGFIPEKDMVGLH
                     ASAICLAYPSLYEGFGLPVVKSLKVGVPIITSNNSSLVEISQGAAILIDPNSTDSIAK
                     AIEKIVHSQQLRRRLSQKGLEIAKKFSWQTTAKETLKVYHSVLS"
     gene            6283..7392
                     /locus_tag="PKZ29_01165"
     CDS             6283..7392
                     /locus_tag="PKZ29_01165"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOG37515.1"
                     /translation="MLIGIDGNEANIVNKVGVGQYAFKLLWHLYQQDKQNHYLIYLKD
                     QPSHDLPPARPNWHYLVFGPSKLWTRFALPLHLLFQKQKLDLFFSPSHYSPIFSRFPT
                     IPTIHDIGYLKFPHQFNQKDLHQLIHWTQQSLRQAKHIITVSQFSKDELIKTYHINPD
                     KITIAYNGVDSPPKINHLAQQKILKTYHLQSGNYFLYLGTLKPNKNIPFLIRSFFRYL
                     QSDKSQPSVKLVIAGKKGWLFDEIFATVQQQGIEDRVIFTDFVTEDQKWTLYQHAITS
                     VLPSTYEGFGIPAIESMKVGTPVIVSNIPPFREVVKTNGLFINPANESDLCQKMIKIT
                     DKKTRQHYSKLGKIQADKFSWTNTASSVIKVFQTL"
     gene            7440..8033
                     /locus_tag="PKZ29_01170"
     CDS             7440..8033
                     /locus_tag="PKZ29_01170"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOG37516.1"
                     /translation="MKDRTGKDLSPNQIFQKVTTRISTIFLEFWLMILRFVGYIPVHS
                     LRKIFYLVSGINLPWDSTIHIGANFFKPSNISIGHDTIIGDHCFLDGRAPLKIGSHVG
                     IASQVLIYNDEHEINSPDYGNSFGPITIGDYVFIGPRAIILPNVKIGTGAVVAAGAVV
                     TKDIPEFEIWGGVPAQKISHRKISRPNYKLGRPMLFQ"
     gene            8033..>8404
                     /locus_tag="PKZ29_01175"
     CDS             8033..>8404
                     /locus_tag="PKZ29_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37517.1"
                     /translation="MTKKIFYFLFVIFNIFALIYLVYPLPSLPDLPNSVKSNLPGDTV
                     QISHVTAYYTNMSRTEVMNFYKAYYTGLFRINLNHPPEKAKQVIVDTIPSYYLEEIVL
                     PLKESLYINGFEWEQDVFTKPE"
BASE COUNT         2565 a         2179 c         1134 g         2526 t
ORIGIN      
        1 ttttcccaaa tcaccttcaa aaaattcacc ccatttgacc agctcttttt tcccagtcga
       61 caaattgtca aacactaccg tcttataatc ttttttactt aactctttgt taactgtaga
      121 accgatatat ccggccccgc cgacaattaa aatcattttt tatttaaaaa aaataaataa
      181 ctgtttaatt ttatcattta ctctctacta ggctttccca aggatagcta ttatccccaa
      241 aatgaccata tttagctgtc tttttataaa ttggtcgttt caaatcaaat ttatcaataa
      301 tccctctaac cgacaaatcc atcagccccc aaccaaaacc ctcaattgtc ctagctgatt
      361 ttctggcggt gccaaaagtt tctattgact ttaccaccgg atctctttga ccaatagcat
      421 aagccaattg gatttcgcat ctgtcagcca aacccgccgc cacaatattt ttcgctatat
      481 atctggcagc atatgctccg cttcggtcta ctttagtcgg atctttgcca gaaaaacatc
      541 cacctccaat tcttcccatc cccccataag tatccactac tatttttctc ccagttagcc
      601 cggtatcact tgtcggtcca ccaaatttcc aacttccggt gccattaaaa acaaccttat
      661 tctcagcaat ttcacccaac ctaaatttat ccagcaacgg taatacagcc agtttaagca
      721 actgtttctt gacttcatcg cccgacaaat ctttttcaaa aggcttggct aatacaatct
      781 tttctactgt ttttggtcta cctctttcat aaaccacgct cacctcagcc ttaccatcag
      841 gtctaagata aggcaaatct gagttcatta gttcatccat ctttcgacaa atattatgcg
      901 acaacattat cggcagcggc attaattctg gcgtttcctt gcaggcatac ccatacatta
      961 ttccttgatc acccgcccca tcattatcca ctcccacagc aatatcacta gactgttgat
     1021 gaaccaaaat ttctacaggg cttttgtgcc aaaatccaaa atcttcatta tcataaccca
     1081 aacccaaaat tacttttctg gccacagtct cataatcaat tccctgccga caattgactt
     1141 ctcctgccac tattagccga ttaaaagtcg ccaaagtctc tactgccacc ctggcatttt
     1201 tatctctcac caaagcttca tccaaaattg tatccgacac ttgatcacaa attttatccg
     1261 gatgaccagc gcaaaccgac tcagacacaa atacggggta attcatattt ttcctttttt
     1321 taaatttata aaggcttgtc tccccaaaac aggaaagcac cgtgaactta ttcaagtctc
     1381 ggttgctgtc gctgacttac gtcaggactc gtgacaatta agctaattat ttaacacttt
     1441 tatccaaatg tcaaaaccaa aattattttt tctgacttcg tttaaaatct ggtgtcagtg
     1501 ttactaatcc gccaaccaaa gaatttatca ctcccaaaat accggcaaac gttgacatca
     1561 acaaaacgct ttctattgac accgcttcct tgccaaaaaa atagacaaac aactgctctc
     1621 tggcgccaaa accggcaaaa gatattggca gggttaatat ggttgatatt accggcaaaa
     1681 aaattaatat cgaaaccacc gacaaaccgg ttccaaaata ccagccgata aaccacattt
     1741 gcagcaccca aaaaatatca ctcataagcg acactcccaa cgacttacct atctgaacca
     1801 aatttttttt gtctaccaat tccaatatac cgctaaattt atttagccac ttaaaattaa
     1861 aaactttttt tatatcaaaa aatattatcg tcacataaaa tgtcagacag cccaacaaca
     1921 atcccgcaaa caccaactga atcatcatcg gtatttccac tcccttgttt cccgccaaaa
     1981 aaatcattat tgatcccaac accacaaaca ccgtcaaccc cacaaaccgg tccaacaaaa
     2041 ttgatcccaa caattttggc ttagaaattt ctgggtattt ttcatcaata ataatccatt
     2101 tcaaagcatc tcctgccacc gccgatgacg gcaaaaacaa accataaaaa gctgccgaca
     2161 aactcgactt aaaaaacaaa attatatcct taaaagtcgg atttttaatt aaaagtaaac
     2221 tccatctcca agaaatcatt cccatcaacc ctaaactaat taatatgttt actaccaaaa
     2281 accaccactc cactctagac aaatctcttg ccaaagagac tacatctact tttttaaacg
     2341 caaaataaat caatatcgac gatatcacca atttgatcaa cttagaattt attattttct
     2401 taaacatgat tattcactag aataacacgt ataataacct taaataaaca tggtcaaggt
     2461 tttgcttctc agtcatattt ttccgccggc tgtcgatggg ggatcacgag ttgttcatca
     2521 actagggcaa tattttgcct ctcaaaattt tgataccatc tatctttctt ctgattgttc
     2581 cagcaccgac gactttgtca aatccaacta ccgtccctcc cgccagctaa cctcccaaat
     2641 caaacttccc gtttatcacc gtctccgccg acctctaaaa ttgattaatc tttttctccc
     2701 ccaaaaaact tatctccacg ggttattgtc tgttttccaa aaaggacccg tctttcgatt
     2761 ttttcctttt ttaaaatcta ttttcacagt tttaaaattc aaacccgact atattgtcgc
     2821 cggccccctg cccactacca ttatttggta tgcccacatt tttaagcacc ttttaaagaa
     2881 attatcccgt catcaggtca aaattttaat taatgcttcc tttcatgtct ccgatcctga
     2941 ttttcatcaa ctcccgctta tccgcagtct tcgtcactcc gactatatct ggactttatc
     3001 ccaattcgaa actgattatt ttatccacca tcttcatctt ccgcccaaaa aaattatcct
     3061 tgccggcaat ggcgttaatt ctggttttat caaaaaaacc acaactccaa accgtctcaa
     3121 gccaaaccaa ccaatcaact tactttttat tggctccatt tctgcccaca aaggtcttga
     3181 taccctcatc gacagttttt cgtatctaat ttcccaaaaa aattctcccc attttcggct
     3241 gattattgcc ggccaaaaaa ctctttatta ccccattatc aaacaaaaaa ttagttctct
     3301 ccccaaacct ttttctacca aaataaaatt tatttttgat tttccccaat cccgcctcaa
     3361 atatttgatt gacaactctt atactctcat tctaccctca cgccacgaaa gtttcggatt
     3421 ggtaattatc gaagcctggg cgcggggcaa accggttatt accagcaata ttgccaccct
     3481 ttcagaactt gtttcccgtg ctaaaggcgg tttaactttt aagcttaaca accaccttga
     3541 tctctcctct caaatcatta aactagtcag ctcgccccaa accgccttta agcttggaca
     3601 aaatggcaaa aaatatgtta aaaataatta tctatggaca aaagtcggac aaaaaatatg
     3661 ccaaaaatta ctatcataat catcatttta atctttgctg ccgtcatcta ttttatctct
     3721 cgaccaaaag ccaaacctca aaattttgat aacaatgtca gcgccatcat caaaggtaat
     3781 ccttattatc tcgaagttgc caaaactttt acttcacgac aaaccggcct ttcccaccgg
     3841 acagatcttt gctccaactg tggcatgttg tttgtttttg atcgcgaagg taaatatcct
     3901 ttttggatga aagataccaa aattcctctg gacattatct ggctaaactc aaaatttgaa
     3961 gtcgtcaaaa ttatcactgc tactgaaact gattctctca aaagctttac caacgaccaa
     4021 ccagctttat atgtcatcga gctaaacgcc aatgaagtct ttaaacttga tctaaaagta
     4081 ggggatatta tccaactttc caacgttacc aatgagtcct aaagccgctc cgtccttaag
     4141 ctactctctt gttattccca attacaacgg ttccgccttt attttagact gtctcaaatc
     4201 aatcttttct gccgtcaaaa actgccccca cacacccttt gagattatcc tagttgacaa
     4261 taattctcac gacaacagtc ctcttatctt caaaaatttt tttcaaaaaa ataaattacc
     4321 aaatttacaa tccaaagtct gttatctcac atccaacacc ggttttgccg ctgccgtcaa
     4381 ttatggcatt aaccgttcaa actattccca tattgttgtt gtgaataatg atatcgttct
     4441 caaatcaaac tggtttaagc ttgtttctga tgctatcgtc aaaaatacta tcccaaaaat
     4501 ttccaccttt tttggcactg ttctcacccg cgacggccaa aaatttgaat ctcaaggtct
     4561 gaagttctac cccaacggcc gctgtcacaa cattgccaat ggtcaaaaat actttacttc
     4621 caaattacct caaactccca agattgtttg gggggcttca gctgctctcg ccgtttaccc
     4681 caaaaaagtc attaaatctg ttgacatgtt tgatcctgac ttttttgcct acgaagaaga
     4741 tgttgatttg tgtctccgtt tttacaaact gggttatcag actatctatg ttccccaagc
     4801 tatttcctac cacttgggcg gggctaccag taaattaatg ggaaactttc gttatcgtat
     4861 ggacgctaaa aactggattt atattatcat caaaaattat tcctcaaaag aaattaaaga
     4921 ttatttttta gccttatcaa tagaaagact cagaaactta tctggactca taaaaagcac
     4981 tatcaggatt tataaattta aatctatttt ttatctccct tttgatattg tccgcaccta
     5041 tggggaagtt attgtcaatc tctccaaaat gatccaaaaa cgccgtcaaa tccaaaaaat
     5101 gataaaatat ggtcattata aaaccacctt atgatcatcg gcattgacgt ctcctccgtt
     5161 tgttaccaga ccggcgttag caactatacc cttaatttgg ttaaaaattt agttaatctc
     5221 gacaagtcca accactacaa actgttttat tcctctctca gaatgccctt gcccgaatca
     5281 atcaaaattc tggggcaaaa agctaatgtc accatccatc atcaccgtct gccgccaacc
     5341 ttattacagt ttgtttggaa tcgactcaga ctttttccta tagaaatttt tattggcaag
     5401 tgcgaccttt ttcatacttc agattggact caacctccta cagtttttgc caaaactatc
     5461 gccaccatcc atgatctcac cccgttttta taccctcagt ggcaccaacc caaggttgtt
     5521 aatgctcatc gtcacaaaat gtattgggcc gtcaaaaaat gccgccacct tatctgtgtt
     5581 tcccaaaaca cccaatcgga tttactcaaa ctttttcccc aagttgatat caagaaaact
     5641 agtgttattt acgaatctgc cgaatccaaa tatagtcaat ttctaaaatt acccgcgcca
     5701 actcaagaca aaaaaaagtt ggtcatcaaa aaacaatatg gcttggaaaa ttttattttg
     5761 gcccagggaa ccagagaacc tagaaaaaat ctcgaccgcc ttatcgatgc ctttctcaaa
     5821 ttcaaacaaa aatatcccaa aaccagaacc cagcttgcca ttaccggcaa atatggttgg
     5881 ggcaaagatg tcaccgtcaa ggacttcagc ataaaaatac ttgggttcat tcccgaaaaa
     5941 gacatggtcg gacttcacgc cagcgctatt tgtctggctt acccctcgct ttatgaaggt
     6001 tttggtctgc cggttgtcaa atcgctcaaa gttggcgtcc ccattattac cagcaacaat
     6061 tcttctttgg tcgaaatttc ccaaggagcc gccatcctga ttgaccccaa ctcaaccgat
     6121 agtattgcca aagccataga aaaaattgtt cactcccagc aactccgccg ccggcttagt
     6181 caaaaaggtc ttgagattgc caaaaaattc agctggcaga ccactgccaa agaaacttta
     6241 aaagtttacc actccgtttt gtcatagctg ataaaatcaa ttatgttaat tggaatcgac
     6301 ggcaacgaag ctaatattgt caacaaagtc ggtgtcggtc aatacgcctt caaacttctc
     6361 tggcatcttt accaacaaga caaacaaaac cattatctaa tttatctcaa agaccagccc
     6421 agccatgatc tccctcccgc ccgacccaat tggcattatt tagtctttgg cccaagcaaa
     6481 ctctggactc ggtttgctct cccgctccat ttattatttc aaaaacaaaa actagattta
     6541 tttttttctc ccagccatta ttcaccgatt ttttcccgtt ttcccaccat tcccactatc
     6601 cacgacatcg gctatctcaa atttccccac cagtttaacc aaaaagactt acaccaatta
     6661 attcactgga cccaacaatc tctccggcaa gccaaacata ttattaccgt ctctcagttt
     6721 tccaaagacg aacttataaa aacctaccat attaatcccg acaaaattac tattgcctac
     6781 aacggtgtcg attccccgcc caaaataaat catctggctc aacaaaaaat tctaaaaacc
     6841 taccatctgc aatcaggtaa ttatttcctt tatctgggca ctctcaaacc caacaaaaat
     6901 attccttttc ttatcagatc tttctttagg tatcttcaat ccgacaaatc tcaaccgtca
     6961 gttaaactgg ttatcgccgg caaaaaaggt tggctgtttg acgaaatttt tgccactgtc
     7021 caacaacagg ggattgaaga ccgggttatt tttaccgact ttgttaccga agatcaaaaa
     7081 tggactctct accaacatgc tattacttct gttttgccca gcacctacga aggttttggt
     7141 attcccgcca tcgaatctat gaaagtcggc accccggtta ttgtctccaa tattcccccc
     7201 ttcagagagg ttgtcaaaac caatggctta tttatcaacc ccgccaacga gtccgacctc
     7261 tgtcaaaaaa tgataaaaat tactgacaaa aaaactcgtc aacattattc aaagctcggc
     7321 aaaattcagg ctgacaaatt tagctggacc aacaccgcta gctcagttat caaagttttt
     7381 caaactttat aaacctttcg ctactggcac aaacctcaga atttaactat aatctgttta
     7441 tgaaagaccg caccggcaaa gatctctccc caaatcaaat ttttcaaaaa gtcaccaccc
     7501 gaatttccac catcttctta gaattttggc tcatgatcct cagatttgtc ggctatattc
     7561 ccgtccattc tctcagaaaa attttttacc ttgtttccgg catcaatctc ccttgggatt
     7621 caaccatcca catcggcgcc aactttttta agcccagtaa tatcagcatt ggtcatgaca
     7681 ctatcattgg tgatcattgt tttttagacg gccgggctcc actcaaaatt ggcagccatg
     7741 tcggcattgc cagccaagtg cttatttaca acgatgaaca cgaaataaat tctcccgatt
     7801 atggcaactc atttggaccg attactattg gtgattatgt ttttatcgga cccagagcca
     7861 ttattttacc caacgtcaaa atcggcaccg gcgctgttgt tgccgctgga gccgttgtta
     7921 ccaaagatat tcccgaattt gaaatctggg gcggagttcc ggctcaaaaa atttcccatc
     7981 gcaaaatcag ccgtccaaat tataaacttg gccgccccat gttgtttcaa taatgaccaa
     8041 aaaaatcttt tatttccttt ttgtcatttt caatattttt gccctaattt accttgttta
     8101 tcccttacct tcactgccag acttgcccaa tagcgtcaaa tcaaatctac ccggcgatac
     8161 ggttcaaata agccacgtca cggcctatta caccaatatg agtcggactg aagttatgaa
     8221 tttttacaaa gcctactaca ccggtctttt tagaatcaat ctcaatcatc cgccggaaaa
     8281 agccaaacaa gttattgttg acaccatccc cagctattat ctagaagaaa ttgtcctccc
     8341 tctcaaagaa tctctctata tcaacggttt tgagtgggaa caagatgttt ttaccaaacc
     8401 cgaa
//
LOCUS       DAOSHV010000016         7702 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_8188_length_7702_cov_5.382634, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000016 DAOSHV010000000
VERSION     DAOSHV010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 7702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7702
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8188_length_7702_cov_5.382634"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="PKZ29_01180"
     CDS             complement(<1..439)
                     /locus_tag="PKZ29_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37518.1"
                     /translation="MVETFNRDRDLLALSNKDKIDSLREFTVSPEGQEKMTKFVDRLR
                     SEVGHDDGWESVCRQAEAELDQYPVTETTLNRLNSTFSAFIDQMPPGHDRGHLSRDLL
                     ASLYLYHSTKNSVKYQSDAAAGLFAGIYHDIGTAIIPRYKDNQF"
     gene            665..1363
                     /locus_tag="PKZ29_01185"
     CDS             665..1363
                     /locus_tag="PKZ29_01185"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOG37519.1"
                     /translation="MRLTVVPQGKQLGWNFIEFAGAATISDESGNRISGNIISIDEVR
                     YANLSSKFGQLTMGFNGVYGQWAFEENGGAVMVPYSVSPQAELYLAGGYEKRLLIDNG
                     TTMFTPPGGFGINQETPENVAKRETLEETGVHINNLIKVGEATPNRAFWIKQSEGNWP
                     LTFFACKVEWSALAEKDGQKYLPVTEGAIAELDKLSKLVFLPAIGAINNTNDGIAIAA
                     FAKTLAAFEIGLIR"
     gene            complement(1485..2879)
                     /locus_tag="PKZ29_01190"
     CDS             complement(1485..2879)
                     /locus_tag="PKZ29_01190"
                     /inference="COORDINATES: protein motif:HMM:NF024791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HOG37520.1"
                     /translation="MSLFSKPKVVIWPKNNSVEIYFGRKDNNFFSLDLNLWEEKTDKD
                     LEGLVYLFNQNKITQASVLIPDDVVATKSFVYDTKIETIDKKEVISLASGFVNFDIDP
                     ESIEYSLIQDGEKTIINAVIYDKSKFDHLKSNLLKTNFVIDAYKPVSAAVSDVVSKIY
                     LSEFFLIYPLNEKDYTLLLSKNNQVYLTANFKGPSLDIQKTINYAQLYFSNPVTKIYY
                     PENKDLDIVTSTEMEKTSYNESQIAQNSNQPGNIPLPVVSQINGIIKSSQDINLSKQP
                     NMNNKKNLVPIIAVIIFSFAIVSFIVYFIMSKNKSSEIIDGSQTDSDAVPTTVIETVT
                     PEPTPTIAEISKDIKIQVLNATDINGQAATLKAKLVSLGFENVAVGNAAVSATTNSVQ
                     VKSATTSAYFESVLSQDFPATYTEDLKSTSAYDAVFTIGTDISGMKTTTTTTVTPTKV
                     ATPTSTKVTPTVVE"
     gene            complement(2985..3467)
                     /gene="rpsG"
                     /locus_tag="PKZ29_01195"
     CDS             complement(2985..3467)
                     /gene="rpsG"
                     /locus_tag="PKZ29_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOG37521.1"
                     /translation="MSRKGASKIRQVEPDAIYNSVLVSKIINATMKDGKRNAAEKQIY
                     QALELIKKDFPKESELEVLERAVENIKPKIEVRPRRIGGAVYQIPTPVRGPRQNALAI
                     RWLINSSRARSNKQYHSFAQKIVAELNDALSSSGAAVAKRMEVERMAEANKAFAHLRW
                     "
     gene            complement(3508..3930)
                     /gene="rpsL"
                     /locus_tag="PKZ29_01200"
     CDS             complement(3508..3930)
                     /gene="rpsL"
                     /locus_tag="PKZ29_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018888218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOG37522.1"
                     /translation="MPTSNQLIKRGRKVRVKKLKTGALRDNFNTVNNRFTKTINPIKK
                     GVVTVVKTMTPKKPHSALRKICKVKLSNRNLVTAYIPGIGHNLAEHSIVSIRGGRVRD
                     LPGVRYHVVRGKFDAAGVTGRMQSRSKYGAKKPGAKTA"
     gene            complement(3937..7653)
                     /gene="rpoC"
                     /locus_tag="PKZ29_01205"
     CDS             complement(3937..7653)
                     /gene="rpoC"
                     /locus_tag="PKZ29_01205"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOG37523.1"
                     /translation="MIDFSGLQIKLASPEEVLSWSHGEVTKPETINYRSWRPEKDGLF
                     CEKIFGPSRDYECYCGKYKKIRFKGVICDRCGVEVTVSKVRRERMGHIALAAPVTHLW
                     YLRNTPSPLGVILDVPQKDLESVVYFTKYLVTKVDDSKRKEALDNLRQHSHQRLEQID
                     VDTKKSIDQKTKEMEKEKADLKKKIKNKDQLNIAQEERDVRLKQEIQKIENKALTEKN
                     RIEGLIKYLEEKIQTIEVLSTISDEESFYLSQFRADIFFTAKMGAESLVEVLSQIDLA
                     QTVKNLKKELAKTSSKIKKKKIMYKIRILNGMIDNNIQPTSMVLMNVPVVPPDLRPMV
                     QLTGGRFATSDLNDLYRRLINRNNRLKKLLKLGAPEIILRNEKRMLQESVDILIDAQK
                     ASKNKRKSIKRVPRSLSDLLRGKKGRFRRNLLGKRVDYSGRSVIVVGPELELDQVGLP
                     KEIALEMFRPFVLRELMATGIAPNIKSAKNLIDHRVTEVYDILEKVTQDKYVLLNRAP
                     TLHKLSIQAFKPVLTDGLAIRLHPCVCKGFNADFDGDQMGVHLPLSEASQKEAKKLML
                     PSKNLLKPADSTPISIPTQGMAVGCYYITSVRSQDLAKESAEGFEGLPIFTNRAEAEL
                     AYQSHKIELRELIAVRIKGKIIKTTFGRIWFNKIIPKEFDFVNEAMAGSKALNDLVVK
                     SLEIAGRVKTVKLIDSLKDIGFKGFTLSGISLSMEDCGYLPEKNDIIHHANKLVAEIE
                     DNFKVGLISNDEKKRLSQNIWLEKTEEIAEKTWNTLDIDSPIRVVSSAGIKRASKDQI
                     KQLSGMRGLMVDPTGKIVQLPTKSNFREGLSVFEYVTGARGTRKGLADTALKTADAGY
                     LTRRLVDAAHISIIRIDDCGTSKYITIDANEKGRERYFTRRILGRVLANDILDPKTKK
                     VILKKGEVLDNNAVDLIEAKQITLVDVRSPITCAAETGLCRQCYGWDLSTRKMVEIGV
                     PVGVVAAQSVGEPGTQLTLRTKHTGGVVGIDVTQGLPRVQELFEIRMPKIPSPLAEID
                     GKIRIKESIDGYEVKLTGKDDKKVAKTITYILPSNANLLVADGDLVGKGTQLSSGSLD
                     VREIMDIKGLEAAQKYLINNIQGVYESQGITIHDKHLEVIVKEMSGKIKIEDPGDTNF
                     LYGQIVSLATFNAANEKVKTRDDGNNKPATGRQILLGLIQSALTTGSWLSAASFQQTT
                     SILTEASLIAEVDDLIGLKENVIIGRLIPVNKDQ"
BASE COUNT         2211 a         1426 c         1725 g         2340 t
ORIGIN      
        1 caaactggtt gtctttgtac cggggaataa tagcagtgcc aatatcatgg taaatgccag
       61 caaataaacc agcagccgca tcagattgat atttaacact gtttttggtg gaatgataaa
      121 gataaagtga agctaacaaa tcgcgggata aatgtcctcg atcgtgaccg ggcggcatct
      181 ggtcaataaa agcagaaaag gtagaattaa gacggttaag ggtggtttcg gtaacaggat
      241 attgatctaa ttcggcttcg gcttgacgac aaacggattc ccagccatca tcatgaccga
      301 cctcagacct aagacggtcg acaaatttgg tcattttttc ttgaccttcg ggagaaacag
      361 taaattcacg cagtgaatct attttatctt tgttggagag agcaagtaaa tctctgtcgc
      421 ggttgaatgt ttcgaccata aagtgattat aacaccacca ttttttatat tataatattg
      481 attttttctt tattataggt tataattggg tgaattatag aaagtaaatt gattgccaat
      541 ttatagaaaa taattaagca ctttaaaatt agacccaaaa ttgatgaatg ggaatgtaac
      601 tatattattt gtagttagat tcccgttcat caaaaagtga attgaggaga aagaggtaaa
      661 aaaaatgaga ctaactgtgg ttccgcaagg aaaacaattg ggctggaact ttattgaatt
      721 tgctggcgcg gcaaccatca gtgacgaaag cggcaatcgg atctcgggga atattatatc
      781 tatcgacgag gtcagatacg ctaatctgtc gtcaaaattt ggtcaactga caatgggatt
      841 taatggtgtt tatggacagt gggcttttga ggaaaacggt ggtgctgtca tggtgccata
      901 ctctgtttct cctcaagcgg agctctactt ggcaggcggc tacgaaaaac gtttgttaat
      961 tgacaatggc accaccatgt ttacacctcc aggcgggttt ggtataaatc aggaaacccc
     1021 tgaaaacgtc gccaaacgtg aaacactgga agaaaccggt gttcacatta acaacctaat
     1081 taaggttggt gaagctaccc caaacagagc cttttggatc aaacaatctg aaggcaactg
     1141 gcctctgaca ttttttgcct gcaaggtaga atggtctgct ttggcagaaa aagatggtca
     1201 aaaatacctc cctgtcactg agggcgcaat tgccgaattg gacaaactgt ctaagttggt
     1261 atttttaccg gcaattggag ctataaataa taccaacgat ggtattgcca ttgcggcatt
     1321 tgcaaaaacg ttggcagcat ttgaaattgg tctaattaga taatttttcc cggtagtggt
     1381 attttgcaga aaactgcaaa tataatttac actaccggga ttttttttga ctaaaaaccc
     1441 cgccataagc ggggtaagtt aataaatcaa caactgttat tctattactc gacgacagtg
     1501 ggggtgactt tggttgaggt gggggtggca accttggtag gagtaacggt agtcgtggta
     1561 gttgttttca taccagagat gtcagtgccg atagtgaaaa cagcatcata agctgaagta
     1621 gatttaagat cttcggtata agtggcagga aaatcctgag acaaaactga ttcaaaataa
     1681 gcggaggtgg tggccgattt gacctgaacc gaattggtgg tagcagagac ggcagcgttg
     1741 ccaacagcta cattttcgaa acctaaagaa accaatttgg ctttgagagt agccgcctga
     1801 ccattgatgt cggtagcatt aagaacttgg attttgatat ccttgctaat ttctgcaatt
     1861 gtaggagttg gttcgggagt aacggtttcg ataacagtgg tggggacagc atcagaatcg
     1921 gtttgactgc catcaataat ttcagacgat ttatttttgc tcataataaa atagacgatg
     1981 aaagaaacaa tggcaaaaga gaagataata acagcgatta tggggactaa gttttttttg
     2041 ttgttcatat taggttgttt gcttaaatta atatcttggg atgatttaat tataccatta
     2101 atctgactga cgactggcaa aggaatattg ccgggttgat ttgaattttg ggcaatttga
     2161 gattcattat aggaagtttt ttccatttca gtagaggtaa caatatctaa gtctttattt
     2221 tcgggataat agattttggt aacgggattg gaaaaataca actgggcata attgatagtt
     2281 ttttggatat caagacttgg acctttgaaa ttagcggtaa gataaacctg attatttttg
     2341 gacaataaca gggtgtaatc tttttcgttt agcggataga taaggaagaa ttcactaaga
     2401 taaattttag agacaacatc gctgacggca gccgataccg gtttgtaggc atcgatgaca
     2461 aaattggttt taagcagatt tgatttaaga tggtcaaatt ttgatttgtc gtagataacg
     2521 gcattaatga tggttttttc accatcttga attagggaat attcgataga ttcggggtca
     2581 atatcaaaat tgacaaaacc ggaggcaaga ctgataactt cttttttgtc gatagtctcg
     2641 attttggtgt cgtaaacaaa agatttggta gcaaccacat catccgggat caaaacggaa
     2701 gcttgggtga ttttgttttg gttaaaaaga tagaccaaac cttcaagatc cttgtcagtt
     2761 ttttcttccc aaagattaag gtcgagacta aaaaaattat tatctttgcg accaaagtaa
     2821 atttcaacag agttattttt gggccaaatg acaaccttgg gtttggaaaa tagtgacata
     2881 tattatatga gtatgactaa atttgaatcc aaaatcaaga tgtaaaaaat taaacttcaa
     2941 caaaaaactc cccaaaaatg gggagttttg gatttatatt ttaactacca cctaaggtgg
     3001 gcaaaagcct tgttggcttc agccatacgt tcgacttcca ttcttttggc aacagcagca
     3061 ccactactac tgagagcatc attgagttcg gcaacaattt tttgggcaaa agagtgatat
     3121 tgcttgttgc ttcgggcgcg agaagagtta attaaccaac gaatggctag agcattttga
     3181 cgaggaccgc gaaccggggt gggaatctga tagacagcgc caccaatccg gcgaggtcgg
     3241 acttcgattt tcggtttgat attttcaacg gctctttcca aaacttctag ttcagattcc
     3301 ttgggaaaat cttttttaat tagttctaat gcctgataaa tctgtttttc ggcagcgttg
     3361 cgcttaccat ctttcatggt agcgttgata attttggaga ccaaaacact gttgtagata
     3421 gcatcaggtt ccacttgtct gattttacta gctccctttc tggacataat tgagttttaa
     3481 gtttataaag ttataaagtt tgtaaagtta ggcagtttta gcaccaggct ttttggcgcc
     3541 atatttactg cgggactgca tccgaccggt aacaccagcg gcatcaaatt tgcctcgaac
     3601 aacatgataa cgaacaccgg gcaaatcccg aacccgacca cccctgatag aaacgatact
     3661 gtgttcagca agattgtggc cgataccggg aatataggcg gtgacaagat tgcggttgct
     3721 aagtttaact ttgcaaattt ttcgcaaagc tgaatgaggc tttttgggcg tcatagtttt
     3781 gaccacagtc accactccct ttttgatagg atttatagtt ttggtaaaac gattgttgac
     3841 ggtattaaaa ttatcccgga gagcaccagt cttgagtttt ttgactctaa ctttgcggcc
     3901 ccgtttgata agttgatttg atgttggcat atttgtttat tgatccttat tgacaggaat
     3961 aagacgtccg ataataacat tttccttcaa gcctatcaag tcatcgactt cggcaattaa
     4021 ggatgcttcc gtcaaaatac tggtagtttg ttggaaagaa gcggcggata accaggaacc
     4081 agtggtaaga gccgattgga taaggcccaa gagaatttgc cgaccggtag cgggtttatt
     4141 attaccatcg tctctggttt ttactttttc attggcagca ttgaaagttg ctaaagaaac
     4201 aatttgacca tagaggaaat tggtatcgcc tgggtcttca atttttatct taccgctcat
     4261 ttctttgacg ataacttcca agtgtttatc atggatagta ataccctggg attcataaac
     4321 accttggatg ttgtttatta aatatttttg agctgcttcc agacctttga tatccataat
     4381 ttctctgacg tctaaagagc cggaagatag ttgagtccct ttgcctacca aatcaccgtc
     4441 agccaccaat aaattggcgt ttgaaggcaa aatataggtg atagttttgg cgactttttt
     4501 gtcgtctttg ccggttaatt tgacttcata accgtcgatg gattctttaa ttctgatttt
     4561 accgtcaatt tcggccaaag gagaaggaat tttaggcata cggatttcga acaattcctg
     4621 aacccggggc agaccttgag tcacatcgat accgacaaca ccgccggtgt gcttggttct
     4681 aagggttagc tgagtaccag gttcgccgac agattgagcg gcaacaacac cgacaggaac
     4741 gccgatttca accatctttc gggtagaaag atcccagccg tagcattgcc gacaaagacc
     4801 ggtttcggcg gcacaagtga tgggtgaacg gacatcaact aaagtgattt gtttggcttc
     4861 aataagatca acggcgttgt tgtccaagac ttcacctttt ttaagaatga cttttttggt
     4921 tttgggatca aggatatcgt tggctaaaac tcgtcctaaa atccgacggg tgaaatatct
     4981 ttcccgacct ttttcgttgg catcaatagt gatgtatttg ctagtgccgc aatcatcaat
     5041 tcggataata gaaatgtggg cggcatcgac taagcggcgg gtaagataac cggcatcggc
     5101 tgtcttaaga gcggtgtcag ctaaaccttt gcgagtgccg cgggcgccgg tgacatattc
     5161 aaaaaccgac aatccttctc taaagtttga tttggtggga agttgaacaa tttttccggt
     5221 aggatcgacc atgagtccgc gcatacctga aagctgcttg atctgatctt tggaggctct
     5281 tttgatacca gcagaagaaa caactctgat aggagaatca atatcaagag tgttccaagt
     5341 tttttcagca atctcttcgg ttttctccaa ccaaatattt tgacttagac gctttttctc
     5401 gtcgttgctg ataagaccaa ctttgaagtt gtcttcaatt tctgccacca gtttgttggc
     5461 gtggtgaatg atatcgtttt tctcgggtaa ataaccacaa tcttccattg aaagggaaat
     5521 cccggagaga gtgaagccct tgaaaccaat gtctttgaga gagtcaatca gtttaacggt
     5581 tttgactcga ccagcaattt ctaaggactt aaccaccaaa tcgttgagag ctttggaccc
     5641 agccatggct tcgttaacaa aatcaaattc tttgggaata attttgttaa accaaattcg
     5701 accaaaagta gttttgataa ttttgccttt gatacggaca gcaatgagct ctcggagttc
     5761 gattttgtgc gactgataag ccagttcggc ttcggcgcga ttggtgaaaa taggcaagcc
     5821 ttcaaaacct tcggccgatt ctttggctaa gtcttgcgac cgaaccgaag tgatgtaata
     5881 gcaacctacc gccatacctt gagtaggaat agaaatagga gtgctgtcag ccggtttaag
     5941 taagtttttg gaaggcaaca ttaatttctt ggcttctttt tgggaggctt cggacagggg
     6001 caaatgaacc cccatttggt ctccgtcgaa gtcggcattg aaacccttac agacacaagg
     6061 gtgaagacgg atagctaaac cgtcggttaa aaccggtttg aaagcctgaa tagacagctt
     6121 atgaagagtc ggagcccggt taagcaaaac atatttgtcc tgagtaactt tttcgagaat
     6181 atcgtagact tcggtcaccc gatgatcaat taagtttttg gcgcttttaa tgttgggggc
     6241 aatgccagta gccataagct ctctgagaac aaaaggccgg aacatttcca gagcaatttc
     6301 tttgggtaaa ccaacttgat ccaactctaa ttcgggacca acaacgataa ccgaacggcc
     6361 ggaatagtcg actctttttc ccaaaagatt gcggcggaaa cgaccttttt tgccgcgtaa
     6421 aagatcagaa agagaccgcg ggactctttt gatacttttg cgtttatttt tggaagcttt
     6481 ttgggcatca atcaaaatat cgactgattc ttgaagcatt cttttttcgt tgcggagaat
     6541 tatttccgga gcgccaagtt tgagtaattt cttgagacga ttgttgcggt tgataagacg
     6601 gcgataaaga tcatttaagt cagaggtggc aaaacgaccg ccagtcaact gaaccatagg
     6661 acgtaaatca ggtggaacca cagggacatt catcaatacc attgaagtag gctgaatgtt
     6721 attgtcaatc atgccgttta gaattctgat tttatacatg attttctttt tcttgatctt
     6781 agagctggtt ttggcaagtt cttttttgag atttttgaca gtttgagcta aatcgatttg
     6841 agataaaact tcgaccaaac tttcggctcc cattttggcg gtaaagaaaa tatcggcccg
     6901 gaattgggat aaataaaagc tttcttcgtc agaaatggtt gataaaactt cgatggtttg
     6961 aattttttct tcaaggtatt taatcaaacc ttcaatccga tttttttcgg tcagggcttt
     7021 attttcgatt ttctgaattt cttgcttgag acggacatct ctttcttctt gagcaatatt
     7081 gagctgatct ttgtttttga ttttcttttt gagatcggct ttttctttct ccatttcttt
     7141 ggttttttga tcgatggatt ttttggtatc aacatcgatt tgctccaggc gctggtgaga
     7201 gtgttgacgc aaattgtcta aggcttcttt tcgcttggag tcgtcgactt tggtaaccaa
     7261 atatttcgtg aaatagacga cagattcaag gtctttttgg ggaacgtcaa gaattactcc
     7321 caagggtgag ggggtattgc gaagatacca aagatgagtt acgggagcgg ccagggcaat
     7381 atggcccata cgctcgcggc gaaccttgga aacggtaact tcgacaccgc agcggtcaca
     7441 aataacacct ttgaaacgaa tttttttgta tttaccacaa taacattcat aatcgcggct
     7501 aggtccaaag attttttcgc agaataaacc gtctttttca ggacgccagc tgcgataatt
     7561 aatggtttcc ggcttggtga cttcgccgtg agaccagctg agaacttcct caggagaagc
     7621 taatttgatt tgaagaccgg aaaaatcgat catattttta aatttaaaca ttattgttcc
     7681 tcctttgttt catcctctaa ct
//
LOCUS       DAOSHV010000017         7630 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_8303_length_7630_cov_6.446601, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000017 DAOSHV010000000
VERSION     DAOSHV010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 7630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7630
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8303_length_7630_cov_6.446601"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..207)
                     /locus_tag="PKZ29_01210"
     CDS             complement(37..207)
                     /locus_tag="PKZ29_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37524.1"
                     /translation="MLKNIAKAIIHKMGYLVIKNKLPSDIESDEEFISVLEKCKDYTM
                     VAADRSYALFGQ"
     gene            complement(220..1590)
                     /locus_tag="PKZ29_01215"
     CDS             complement(220..1590)
                     /locus_tag="PKZ29_01215"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOG37525.1"
                     /translation="MENQNNIKPVVSIVKTAPSYEELSRNGAMKNALLDLLGGLNLSK
                     QNPFGEYVKAGQTVLIKPNWVREANPLGYDLDSLITHTVLIKEVINLLAIALGNRGKI
                     VMADAPLQKCNFEILRQRAGIDDMLKEMKLAYPNISFVIEDWRITTLNDVHSKDKEPQ
                     KIKNDVSDSYVVYDLGKESFLEEISEYADRFRVTDYKPSLMRKHHGPGKHEYAITKRV
                     FDVDFIINLPKMKTHMKTGITCAMKNLVGINGHKEFLPHHIKGSFLEGGDCYMAYNPL
                     RKLYENLYDYMWENLNFLSVWKRKILIKVMRYLLLFSRIGKENIQAGSWRGNDTIWRT
                     VIDLNHILYFHSKKQRKVLNIVDGIIAGEKQGPLSPTPKPIGILIAGENPAYIDAVAA
                     KMMGYNIFRIPMIYNAIYSRRSKFGGVFLEDLPISFFSENKREPVHFNDITNYNFQKP
                     NYWEGV"
     gene            complement(1592..2350)
                     /locus_tag="PKZ29_01220"
     CDS             complement(1592..2350)
                     /locus_tag="PKZ29_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp fold amidoligase family protein"
                     /protein_id="HOG37526.1"
                     /translation="MENPSSFSEKVAWKKIYDRDPLLRVTADKLLVRKYIAEILGEKQ
                     ANEILIPMLAQARTIDKINFDDLSGDYIIKTNHDSGGNLIIREGQKVRREDKSRLKKL
                     SKLPYGTFKHEWAYQGIKPSFIVERLMLDEAGGIPKDFKFYVFHGKCHLVHVDFDRFT
                     NHTRSLYTPEWKYLKIMLKFPQGPDIPRPQNFEKMLQLAEKLGQDFGFVRVDLYSIGD
                     RIYFGELTHYPGSGLELFTPLEFDFELGGHWHLK"
     gene            complement(2481..3686)
                     /locus_tag="PKZ29_01225"
     CDS             complement(2481..3686)
                     /locus_tag="PKZ29_01225"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOG37527.1"
                     /translation="MVGKAPPQKVFEKRLPIGPSLGLYKMNNQKIKIVIVINDFLVGG
                     AQKQLVELLPYFDRSKFDFSLITLFYFEKRANLYNKLPEWLDVYQLKFKNFHDLRSWK
                     ELYGVIAEIKPDIVMSSLFFSNTIIRLIKPILRYKVITREHNTYTEKNFIQRLIDRLL
                     AHLSDKIVAVSKGVADFTSRQEHIPITKFTVIHNGISVDNIENYRSHLPDKNTLKNSL
                     GFREQDKILINVARLTPQKNQRLLLNAFAKFALENPEYKLLILGEGPLFGELNELIIK
                     LKFGDQISLVGHVENPLDYYLIGEFFISTSKIEGLSNAYLESLACGLPLLSTKTAGTD
                     ELITEGENGFFINEHSVAGVLSGINKMLNKDLKTMGRFAREKVKNFDISVTANNYETL
                     FINCYENKI"
     gene            complement(3608..4249)
                     /locus_tag="PKZ29_01230"
     CDS             complement(3608..4249)
                     /locus_tag="PKZ29_01230"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOG37528.1"
                     /translation="MFNAPSLLRIEENKALSAIRLEGSVLDLGGEKDSDYLKLIGGQF
                     NVLTLNNDPAARADINHDLEKELPFEDSSYDNVLLINVIEHIFNFRLLLAEAVRVLKP
                     KGKLIIVVPFLFPIHPSPNDHWRFTSQALEGEVRAVGVRELKITFLGSGVFASCFLFI
                     DRLLPKFLRYLNYKILRHLVVSLDAAWSWLAKLLRKKYSKNDYPLGLVLVCTK"
     gene            complement(4254..5267)
                     /locus_tag="PKZ29_01235"
     CDS             complement(4254..5267)
                     /locus_tag="PKZ29_01235"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOG37529.1"
                     /translation="MKLLVITQKVDKNDSVLGFFHRWLEEFARHVEQMTVICLERGEH
                     RLPGVKVLSLGKEGGRSRLKYVWRFYKYIWQERKNYEAVFVHMNQEYILLAGWLWFLL
                     GKRIYLWRNYHYGNLWTRLAVAFCARVFCTSAFSYTARFKKTILMPVGVETNFFVDKG
                     LIREEKTILSLGRLAADKRLDILLEAVAKVPAITKLDFFGDELPRGLGYLVKLKELSD
                     RLQISSKVSFNSGIPNDQTVDVYNEHEIFVNLSPSGMYDKTIFEAGACGCLVISSSSD
                     WGKIADQRLYFDGTAVDLAKKIATLVALSKEEKALLVNHCKSLSKQHSLGALGDRLVG
                     LLY"
     gene            complement(5400..6530)
                     /locus_tag="PKZ29_01240"
     CDS             complement(5400..6530)
                     /locus_tag="PKZ29_01240"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOG37530.1"
                     /translation="MVNVLMISRDRNMFDSSSDVRERIKLYFGSVQQIHIIVFAKSSL
                     GLSPQKIADNAWLYPTNSLTRWTYIIGAAKIGRNIIRNSFPSQRESLITAQDPFDCGL
                     AAIILGKLCKLPIEIQLHTDPFSTYFSGILNFVRKELVKFTLPRSQGLLAVSEKLADE
                     VSSRFNYNRSNIMVLPVYIDAEKLLSKQSGAIKVQGQSKTIMVVARLSPEKNVALAIK
                     ILAEVRKSTDARLVVVGSGPEADKLKNLAEQLQVSDFVSFEGWRNDVTPYYKGADVFL
                     QTSYFEGYGMALVEAGLNHLPVISTPVGLAGELINGKEIIICPQDDEKQFARSVLSIL
                     ANPSLSRVLADNLFDRLDRDIKSKQEYMSLIKSHWEKIVSNT"
     gene            complement(6523..7542)
                     /locus_tag="PKZ29_01245"
     CDS             complement(6523..7542)
                     /locus_tag="PKZ29_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37531.1"
                     /translation="MKKKEKISIIMSHGGVGDFLFQLDLAKRLELAGVRSIFLVRKNR
                     FFLNDILLNSNVVSAKIVRADGLYYLFSTLYVWLLCLVKKVFIINSFHFTCLRLPTRI
                     FYRVAKKLSANIIICKKEIDPVYPYKQIIYHEGENIWQRNNRIVVEVGNAAAGVGFPV
                     LDFGSVDIEKGDYLHLHPVGSRLEKSYPAAKLVKLLKLFDKSQKVVITLTPNEGKWYL
                     AEELVSYVDSQPNLTLIVKHFSFKEIKNLIAGASVFCTVNTGLLWVGIMLGKKVVVCD
                     TFTDFEWNPQHYGPVKRIVHSHDEQGNSLHLKLVKHQDGEYYESMYRITPSEIYYEIS
                     ALLNG"
BASE COUNT         2342 a         1725 c         1255 g         2308 t
ORIGIN      
        1 caaaatcacc gggaaccgca gccccaacaa catatttcac tgcccaaaaa gggcataaga
       61 cctgtctgcg gcaaccattg tataatcttt gcatttttca agaaccgaaa taaattcttc
      121 gtcactttca atgtctgatg ggagtttatt ctttattact aaataaccca tcttatgtat
      181 tatcgcttta gctatatttt ttagcatatt aataaataat taaactccct cccaataatt
      241 tggtttttga aaattgtaat ttgtaatgtc gttaaaatga accggttctc tcttgttctc
      301 ggaaaaaaat gatattggca agtcctcaag aaaaactcct ccgaactttg accttcttga
      361 atagattgca ttatatatca tgggaattct aaatatgtta tagcccatca ttttggcggc
      421 caccgcgtca atatacgcgg ggttttctcc ggcaattaaa ataccgattg gcttcggagt
      481 cggacttagg gggccttgtt tctcgcccgc gataattccg tcaacgatat ttaaaacctt
      541 cctctgcttt tttgaatgaa aatacaagat gtggtttaaa tcaattaccg tccgccaaat
      601 tgtatcgttc cccctccaac tgcctgcttg gatgttttct ttgccaatcc tagaaaacaa
      661 aagaaggtac ctcattacct ttatcaaaat ttttcttttc caaacagaca aaaaattcag
      721 attttcccac atataatcat aaaggttctc gtataattta cgcaacggat tatacgccat
      781 ataacaatcc cctccctcaa gaaaagagcc cttaatatgg tgcggcaaaa actctttgtg
      841 accatttatg cccaccaagt ttttcattgc gcaagttatc ccagtcttca tatgcgtttt
      901 cattttggga agatttatta taaaatccac atcaaaaacc cttttggtga ttgcatactc
      961 atgtttgccc gggccatgat gtttccgcat caaactgggc ttgtaatcag taaccctgaa
     1021 tcggtcagca tactccgaga tttcttccaa aaaactttct tttcccaagt cataaaccac
     1081 ataggaatct gaaacatcat ttttaatttt ttgcggttct ttgtctttcg aatggacatc
     1141 attcaaggtt gttatcctcc aatcctctat aacaaaacta atatttggat aagctaattt
     1201 catttctttt agcatatcgt caattcccgc tctttggcgt agtatttcaa aattacactt
     1261 ctgcagtggc gcatcggcca tgacaatctt gcctctattg cccaaagcaa tcgccaataa
     1321 atttattact tcttttatta gcacagtgtg agttatcaac gaatcaaggt cgtaacccaa
     1381 gggattggct tcgcgaaccc aatttggttt aatgagcacc gtctggccgg cttttacata
     1441 ctcgccaaaa ggattctgtt tacttaaatt caaacctccc agtaaatcca gcagagcatt
     1501 tttcatcgcc ccatttctag acaactcctc gtaactggga gcggttttga cgatagaaac
     1561 aacgggcttt atgttatttt gattttccat atcacttcaa atgccaatgt ccacccagtt
     1621 caaaatcaaa ctcaagtggt gtaaataact ccaggccact gccaggatag tgggttaatt
     1681 caccaaaata aatacgatcc ccaattgaat acaaatcgac ccttacaaac ccaaaatctt
     1741 gtcccaattt ttcggccaac tgtaacattt tttcaaaatt ttgcggtctg gggatgtccg
     1801 ggccttgagg gaattttagc atgattttca gatatttcca ttccggagta tatagacttc
     1861 ttgtgtggtt tgtaaatcga tcaaaatcca catgaaccag atgacacttt ccatggaaga
     1921 cataaaactt aaaatcttta gggattcctc cagcttcatc aagcatcaat ctctccacaa
     1981 taaaactcgg cttaatacct tgatatgccc attcatgttt aaatgttccg taagggagtt
     2041 tgcttaattt ttttaacctc gacttgtcct ctcgacgaac cttttgtcct tctctgataa
     2101 tcaaattgcc accagaatca tggtttgttt tgatgatata atcaccactt aaatcatcaa
     2161 aatttatttt atctattgtt cgggcctgag ccaacattgg tattaatatt tcatttgcct
     2221 gtttttctcc aaggatttca gcgatatatt ttcgaactaa aagtttgtcg gccgtaactc
     2281 ttaaaagcgg gtccctgtcg tatatctttt tccaggcgac tttctcgcta aaacttgacg
     2341 gattttccaa gttaagttcg tagcccaggc tatttttaaa cctctttttt tcgtgtcggt
     2401 atccagtcaa ggactccccc ataagaaaaa tggattttct aattaaaaag atcggccaag
     2461 agttccaaac ctttttgaat ttagatttta ttttcatagc aattaataaa tagcgtttca
     2521 taattattcg ccgtaacaga tatgtcaaaa tttttgactt tctcgcgagc aaagcgcccc
     2581 attgtcttta aatctttatt caacatttta ttaataccgc taagcacccc agcaacgctg
     2641 tgttcattta tgaaaaaacc attttccccc tccgtgatca attcgtcagt ccctgccgtt
     2701 ttagttgaca acaagggcaa gccacaagcc aaggattcaa ggtaagcatt tgacaaaccc
     2761 tcaattttag aagtggaaat aaaaaactcg ccaataaggt agtaatccaa gggattttcc
     2821 acatgcccaa ccaaagatat ttggtctcca aacttgagtt ttattattaa ctcattcaat
     2881 tccccaaata acggaccctc gccgagaatc aacaacttgt attcggggtt ttccaaagca
     2941 aatttcgcaa acgcgtttaa caaaagtctt tggttttttt gcggcgtcag cctagctacg
     3001 tttattaaga ttttgtcttg ctcccgaaat cccaacgaat tttttagggt atttttgtca
     3061 ggcaaatgag agcgataatt ttcaatattg tctacactta tgccattatg aataactgtg
     3121 aattttgtaa ttgggatatg ttcttgtcgt gacgtaaaat ctgccacccc cttggatacc
     3181 gcgactatct tatcggaaag atgggccagg agtctgtcaa tcagtctttg gatgaaattt
     3241 ttttcagtat aggtattatg ttctctggta atcactttgt aacggagaat cggtttaatc
     3301 aacctaatta ttgtgttgct gaaaaaaaga cttgacatta ctatgtcagg ttttatctca
     3361 gcgataaccc catataattc tttccacgac cttaggtcat ggaaattttt aaactttagc
     3421 tgataaacat ctaaccattc gggtagtttg ttatacagat ttgcgcgctt ctcaaaataa
     3481 aaaagcgtaa tcaacgagaa atcaaactta cttctatcaa aatatggcaa caactccaca
     3541 agttgttttt gcgccccgcc aacgagaaaa tcattaataa cgattacaat ttttattttc
     3601 tgattgttca ttttgtacag accaagacta ggcccaatgg gtaatcgttt ttcgaatact
     3661 ttttgcggag gagctttgcc aaccaagacc aagcggcatc caaagacacc actaggtggc
     3721 gtaggatttt atagttcaga taacgtaaaa attttggaag caaccggtca ataaataaaa
     3781 agcaggacgc aaacacgcca gaacccaaaa aagttatttt cagctccctg acgccgaccg
     3841 cccttacctc tccttctaac gcctgactgg taaatcgcca atgatcattt ggggatggat
     3901 ggataggaaa aagaaaggga acaacaataa tcaatttacc ctttggtttc aaaacacgga
     3961 ccgcttcagc caacaggagg cgaaaattaa aaatatgttc aattacgtta atcagcaaga
     4021 cattgtcata agatgaatct tcaaaaggga gttctttttc caggtcgtga tttatatctg
     4081 ctctagcggc tggatcgtta tttaaagtca gaacattaaa ctgcccccca ataagcttga
     4141 ggtagtcgct gtccttttca cctcctaagt ccaagacgct tccttcaagc ctaatggctg
     4201 ataaagcctt gttttcttct atcctcaata aagaaggcgc attaaacata aggttagtat
     4261 aacaaaccga ccagacggtc tccaagcgct cccaacgagt gttgtttaga caaactttta
     4321 caatggttca ccaataaagc cttttcttct ttgctcaagg caaccaaagt ggcaatcttt
     4381 ttggccaaat caactgctgt cccatcaaaa taaagtctct ggtctgcaat cttgccccaa
     4441 tcactgctgg aactgattac caaacatccg caagcgcccg cctcaaagat tgttttgtca
     4501 tacattccag acggactaag attaacaaat atctcatgtt cattataaac atcaaccgtt
     4561 tgatcattag gaatacccga gttaaatgaa accttacttg aaatttgcag tcggtcgctc
     4621 aattccttca acttaaccaa ataaccaagc ccccgtggta attcatctcc aaaaaaatca
     4681 agttttgtta ttgccggaac ttttgccact gcttcaagaa gaatatccag cctcttgtca
     4741 gcagccaatc tcccaagaga taagatggtt ttctcttccc gaatcaaacc tttatcaaca
     4801 aaaaaattgg tttccacccc gaccggcatt aaaattgttt ttttaaatct ggccgtgtaa
     4861 gaaaaagccg aggtacaaaa aaccctcgcg caaaacgcta ccgccaaacg agtccaaaga
     4921 ttgccatagt gatagttacg ccacagatat atccttttgc ccaacaaaaa ccagagccaa
     4981 ccggctagca aaatatattc ttggttcatg tgaacaaaaa ctgcctcgta attttttcgt
     5041 tcttgccaaa tatatttata aaaacgccaa acgtatttaa gacgggatcg ccccccttct
     5101 ttgcctaatg acaaaacctt gactccgggc aagcggtgct cacctctctc caaacagatg
     5161 actgtcatct gctcaacatg tctggcaaat tcttccagcc aacgatggaa aaagcccaaa
     5221 actgagtcat ttttgtctac tttttgtgtg attaccaaaa gcttcacaag gttgattgta
     5281 gcacaaaaaa acggccccta aaaattggaa gcgaaaatta gcctaatcaa cctcggccca
     5341 tttatggcaa aaaatccttt aaaatcttcc ggcgcgcccc acatttttct tggatagttt
     5401 tatgtgtttg aaacaatttt ttcccaatga ctttttatca aactcatata ttcctgcttt
     5461 gatttaatgt cacggtccaa cctatcaaat aaattatcgg ccaagacgcg agacaaagac
     5521 ggattagcca aaattgacaa aacagaccga gcaaactgct tctcatcgtc ttgaggacaa
     5581 attattattt ccttgccgtt aatcaattca ccagccaaac caactggcgt gctaatgacc
     5641 ggcaaatggt tcaaaccagc ttccaccaaa gccattccat atccttcaaa ataagacgtc
     5701 tgaagaaaaa catccgcccc tttgtaataa ggagtaacat cattgcgcca accttcaaaa
     5761 ctgacaaaat cagaaacttg aagctgttca gccaaatttt taagcttgtc cgcttcgggc
     5821 ccggaaccga ccacaacaag cctggcatca gtagacttcc ttacctcggc aagtatttta
     5881 atagccaacg ctacattttt ttcaggtgac aacctggcaa caaccattat tgttttagac
     5941 tggccttgaa cctttatcgc cccactttgt ttggataaca acttttcggc atcaatataa
     6001 acaggaagaa ccattatatt tgaacgatta taattaaatc gagaggaaac ttcatcagct
     6061 aatttttcac tgacggctag caaaccttgg gaacgcggca aggtaaattt gaccagttct
     6121 tttcgcacga aattcagaat tccggaaaaa taggttgaga atgggtcagt gtgcaactgg
     6181 atttcaatcg gcaatttaca taatttgccg agaattatcg cggctaggcc acagtcaaaa
     6241 ggatcttgcg ctgtaatcaa agattctctt tgcgaaggaa aactgttcct tatgatgttt
     6301 ctgccaattt tagcggcgcc tataatgtag gtccaacgcg tcaacgagtt ggttgggtaa
     6361 agccaagcgt tatcggcaat tttttgcgga cttaacccca aagaactttt ggcaaatact
     6421 ataatgtgta tttgttggac tgacccaaaa tacaatttta ttctttcacg tacgtcgcta
     6481 cttgaatcaa acatatttct atctcggctg atcatcaaaa cgttaaccat tgagaagggc
     6541 actaatctca taataaattt ccgagggagt tattcgatac attgactcat aatattctcc
     6601 atcttggtgt ttaactaact tcaaatgaag agagttccct tgttcatcgt gactatggac
     6661 aattctctta accggaccat agtgttgtgg gttccattca aaatcggtaa aagtatcaca
     6721 aacaaccacc tttttaccta acattattcc aacccacaac aaaccggtat tgaccgtgca
     6781 aaaaacagag gcgccggcga tcaaattctt tatctccttg aaagaaaagt gttttacaat
     6841 caaagtcaaa tttggttggg aatcgacata agaaacaagc tcttcggcaa gataccattt
     6901 tccttcattt ggggttaggg taatcaccac tttttgcgat ttatcaaaca attttaataa
     6961 cttcaccaac ttagcggcag ggtaagattt ttccaaccga gaaccgacag ggtgaagatg
     7021 taaataatca cccttttcta tatcaacgga cccaaaatcc aacaccggaa aaccgacacc
     7081 cgccgccgca ttcccaacct cgacaacaat ccggttattt ctttgccaaa tattttcgcc
     7141 ctcatgataa ataatttgct tgtatggata aactgggtca atttcttttt tgcaaataat
     7201 gatattggct gacaactttt tggccactcg ataaaatatt ctcgtcggca atctcaaaca
     7261 agtaaaatga aaagagttga taataaaaac tttttttaca aggcaaagca accacacata
     7321 aagcgttgaa aacaaataat aaagaccgtc agccctcact attttggcgg aaacaacatt
     7381 gctattcaac aaaatatcgt tcaaaaaaaa cctatttttt ctgaccaaaa aaatagacct
     7441 gacccccgcc aactccaatc gcttagccaa atcaagttgg aacaaaaaat cccctacccc
     7501 gccatgagac attatgattg aaattttttc tttctttttc atttttcaaa aatttcggca
     7561 agtttgattg caattttgtc ccagttatat ttttcaacca ccaactggcg agctttctca
     7621 accacttggt
//
LOCUS       DAOSHV010000018         7511 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_8490_length_7511_cov_5.013948, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000018 DAOSHV010000000
VERSION     DAOSHV010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 7511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7511
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8490_length_7511_cov_5.013948"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..681
                     /locus_tag="PKZ29_01250"
     CDS             76..681
                     /locus_tag="PKZ29_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37532.1"
                     /translation="MKNNKLAKILIPIIAVIVVIESIILVSNLDKSNTGGVANQSSQL
                     SPVLEKEVDNPVVDFVFATDTKEMKVGKSYEVVLNLVAKKDFNLDGLEAYINYDSQLL
                     TVSKLAQGTALPKADKLENMSGMVKSIFFIETPKEGYAVKTGEIVKVLSFEVTPKAVG
                     VSTLELGTGNEGKQFVTMVVENTTSKQLPFSGNKLEINATK"
     gene            684..1397
                     /locus_tag="PKZ29_01255"
     CDS             684..1397
                     /locus_tag="PKZ29_01255"
                     /inference="COORDINATES: protein motif:HMM:NF013156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HOG37533.1"
                     /translation="MTKTAYLLNTIVILLLLMTASVKAASAAPYLYFEPAAGNYSLEE
                     SFSVTAKINSGTETVGGADGVGVYDSTRLELVSITQAPNMVFSSNDGGGSCRIDTASV
                     GKFGFTCTSDFTFGDKPVNGDLVVFNFKAKAIGTAPVTFNCTSGSTTDSNIVKTSTAM
                     DVISCSENINGSYVIGQGTGSPAPTSTPAPSTSTTTSTSTTTTTTAELPKTGAVGTTV
                     GLVIFGIVSFASAVFLGRL"
     gene            1394..2086
                     /locus_tag="PKZ29_01260"
     CDS             1394..2086
                     /locus_tag="PKZ29_01260"
                     /inference="COORDINATES: protein motif:HMM:NF027967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOG37534.1"
                     /translation="MKGKINTKTIVLIVVSLAVIGLLVFGINQIRTMMSGASSDVEPQ
                     GVTVVLGDDGKTATITWTSNKESMGKVEYGTTAASLVLMSAESSMAMDHRVLLSSLQP
                     GKTYHYRIRIGEEIFDNGGIPYNFKTKADKPDSTPTVTPTAVTSQATGSAIICDAKTD
                     YNKDGIVNSFDLMACKKDGGKVVATSPAPASTIRTTTPSTINCDNNTVDYNSDGVINS
                     IDKINCLQSKRR"
     gene            2127..2348
                     /locus_tag="PKZ29_01265"
     CDS             2127..2348
                     /locus_tag="PKZ29_01265"
                     /inference="COORDINATES: protein motif:HMM:NF013342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOG37535.1"
                     /translation="MPIIVEKKKGESKDDIIGKFRRLYLEEDITEEVRKRTAYVKPSE
                     ERYAKKKIIVWRDKCRRRAKNSRRHDVKR"
     gene            2335..2772
                     /locus_tag="PKZ29_01270"
     CDS             2335..2772
                     /locus_tag="PKZ29_01270"
                     /inference="COORDINATES: protein motif:HMM:NF020977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HOG37536.1"
                     /translation="MLKDKLKRDSTEALKNHDQKMVDTLRFLISLIDKKELSLPVGEM
                     TEAEEVRVLRKELKNKEESREMFVKGGRNDLVAQLDYEIEIVKSYLPAEITEDQIKKV
                     VDEIIGGKENNFGLVMKEVMTKLGGEVEGSTVARIVKEKLSGN"
     gene            2773..3147
                     /gene="ybeY"
                     /locus_tag="PKZ29_01275"
     CDS             2773..3147
                     /gene="ybeY"
                     /locus_tag="PKZ29_01275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOG37537.1"
                     /translation="MTNKDGILIRHNKNWGLSESLVKKIAKKVLRERGFGDEVELSIY
                     FVGRKKAKELNKNYRRMDYIPQVLGFPMSTTPGVDDKVRLGDIVICTQKLKYEVEFQK
                     SSVERVMEDWLRHGLDNLLKKD"
     gene            complement(3498..3683)
                     /locus_tag="PKZ29_01280"
     CDS             complement(3498..3683)
                     /locus_tag="PKZ29_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37538.1"
                     /translation="MPGEAIPAGKVIGPGIFVGIAVGKVVDRGTDVGCVTDEGFGAVV
                     DPDDTPNIELTLTPSTE"
     gene            3691..4698
                     /locus_tag="PKZ29_01285"
     CDS             3691..4698
                     /locus_tag="PKZ29_01285"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HOG37539.1"
                     /translation="MTGLVSLYSGEKQKISVVLNGVEGGGLVWTSGNQNVVRVDNLGY
                     ATAVAPGVTTIKVQSGKSVAVVPVWVREVGSLRSVIVVPTVMVTLAPAAPTLIPTLVP
                     TRVPTAISTAIPTLVPTVIPTGVPTLVPTLIPTVIPTLVPDGELTPKISFRFSLTGIK
                     PGSVCISSIGPLEVEVVNRSSGTYQGGLVARFKPVEGEIDSLGDQVFEVSNLFLDKSK
                     FGGVDETNFLKIKGPFHSKNKMCQEGQSGKLSENTVCNLKLDGSKVYDFSNYALAPGD
                     VNYDGVINSIDYSQTKKMMNESEMENAVCGIKGDFNLDGMVNSVDMGLIKMSLSQIDD
                     E"
     gene            4758..6311
                     /gene="dnaX"
                     /locus_tag="PKZ29_01290"
     CDS             4758..6311
                     /gene="dnaX"
                     /locus_tag="PKZ29_01290"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR02397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOG37540.1"
                     /translation="MSVFHLKYRPRRWSDLDLADVADKLNKILSAKDKPQSFLFAGPK
                     GSGKTSAARILAKTVNCLKPEKGEACNSCDNCEEIDKGRAIDIIEMDGASNRGIEDVR
                     NLKDKVYLSPSKLKYKVFIIDEVHMLTKDAFNALLKLIEEPPKHTMFVLCTTDAEKIP
                     DTVMSRLVRIDFRRGGREELRHSLDKVIKGEKIVLDKTGIDFILDRSDGSFRNLQRSL
                     NEIVLNFGTELTLTEVKKFFLDKGGSYNEADFEADLILGKAGVILEKLEKMADEGRDL
                     TDFRQRLMKYFQTKVLAVFGVGEEIKSKMSVTELQRWIELLIRCARQEKETEIDQLPL
                     QLAVIDFLEDESGGEEAKRVETEINDEKKNKTKAEPAPKARAVEKKEESKTEIINIDV
                     SGVSLEKISQFWGNVLMAIKPFNHSVEAFLRASRPIKLDGRHLVVEVFYPFHKDRLEE
                     PKNRQIVEMGLQKALGVELVFDCILSKSKKQPLVIKNDTPIESVSEELVESKKDKADL
                     YDVAKDIFG"
     gene            6389..6637
                     /locus_tag="PKZ29_01295"
     CDS             6389..6637
                     /locus_tag="PKZ29_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37541.1"
                     /translation="MFDKAKMLAQAYKLKKAVEAEVVEIEENGVKVSVSGDQKVKFLS
                     VNGMENKVLVDTINKALKKSQETAAKRMKEMGGLEGLF"
     gene            6673..7287
                     /gene="recR"
                     /locus_tag="PKZ29_01300"
     CDS             6673..7287
                     /gene="recR"
                     /locus_tag="PKZ29_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011024567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOG37542.1"
                     /translation="MAEFPKSIKNLIEAFERLPGIGPKGATRLVFYLLNTPKAYVQDL
                     ATTLIKMKDEIKICRECFSVTEQDVCSICRDKQRNSWQICVVERAIDVMAIENMGGYK
                     GVYHVLGGVINPLEHVGPEELKIDELVERVKNKLNGEGDCLEVILATNPTMEGEATAL
                     YIKKRLEGLKVKISRIGSGLPIGADLEYADQATLCRALDGRRDI"
     gene            7295..>7511
                     /locus_tag="PKZ29_01305"
     CDS             7295..>7511
                     /locus_tag="PKZ29_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37543.1"
                     /translation="MTKKYIAVVIIIVALVAAGIFWANPKDKSKQGLTEDKISSTEML
                     EITMEPTEVKGKIEGKVCYPSSFIPEGK"
BASE COUNT         2446 a          981 c         2075 g         2009 t
ORIGIN      
        1 ctattgatat ggatttagtc aaatattctt tatctgagat agatgatgaa taaaattaat
       61 aattaacctg ccaaaatgaa aaacaataaa ttagccaaaa tattaatacc gataattgcc
      121 gtaatagtgg tgattgagag tataatttta gtatcgaatt tagataagag taatactgga
      181 ggagttgcca accagtcttc acaattgagt ccagtgttgg aaaaagaagt tgacaatccg
      241 gtggtagatt ttgtgtttgc gactgatact aaagaaatga aagtgggaaa aagttatgaa
      301 gtggttttga atttagtggc gaaaaaagat tttaaccttg atggattgga agcatatata
      361 aactacgatt cccaattact gacggtatca aaattagctc aagggacggc tttgcccaag
      421 gcggataagt tggaaaatat gagtggaatg gtgaaatcaa tattttttat tgaaacacca
      481 aaggaaggtt acgcggttaa aaccggagaa attgttaagg tgttatcttt tgaggtgaca
      541 ccaaaagcgg tcggggtatc aactttggaa ttgggtactg gtaatgaggg taaacaattt
      601 gttactatgg ttgtagaaaa taccacgagc aagcaattgc ctttctctgg taataaatta
      661 gagattaacg caactaaata gtcatgacca agactgccta cttacttaat acaatagtaa
      721 ttttgttatt gctgatgaca gctagtgtta aagcggcttc agcggcgcct tatttgtact
      781 ttgaaccggc ggcgggcaat tactctttgg aagagagttt tagtgtgaca gctaaaatta
      841 attcagggac agagacggtc ggtggagctg atggggtagg agtttatgat tctacgagat
      901 tggaattggt gtcgataact caagctccaa atatggtttt ttctagcaat gacggcggcg
      961 gcagttgtcg aatagatact gcttcggtag gaaagtttgg ttttacctgc acttctgatt
     1021 ttacatttgg agataaaccg gttaatggag acttggtagt ttttaacttt aaagctaagg
     1081 caataggaac agcaccggta acttttaact gcactagcgg gtcaacgact gattctaata
     1141 ttgtcaaaac atcaactgca atggatgtta tatcttgcag tgaaaacata aacggcagtt
     1201 atgtaattgg tcagggaaca ggctctccgg caccgacttc aacgccggca ccatcaacat
     1261 cgacaacgac atcaacatca acgacaacaa caaccacggc agagctacca aagactggag
     1321 ctgtaggaac tactgttggt ttggttattt ttggaattgt tagtttcgct tcagcagtat
     1381 ttttgggacg gctatgaagg gtaagattaa tacaaagacg attgtattga tagtggtttc
     1441 tttggcagtg attggtctgc tagtgtttgg aattaatcag ataagaacaa tgatgagtgg
     1501 ggcctcaagt gatgttgaac ctcaaggggt aacagtagtt ttgggcgatg atggaaaaac
     1561 ggcaacaatt acctggactt ccaataaaga gtcaatgggc aaggtggaat atgggacaac
     1621 agcagccagt ttggtgctga tgtcggctga aagttcaatg gcaatggatc atcgggtgtt
     1681 actatcatct ttgcagccag ggaagacata tcattatcgg attagaattg gtgaggaaat
     1741 atttgataat ggaggaattc cttataattt caaaactaaa gcagacaaac cagactcaac
     1801 tccgacggtg actccgacag cagtgacgag tcaggcgact ggcagtgcaa taatatgcga
     1861 tgctaaaact gattataata aagacgggat agttaattct tttgatttga tggcttgcaa
     1921 gaaagatggc ggcaaagtgg tggcaacttc accggcaccg gcgtcaacaa ttagaactac
     1981 gactccaagc acgattaact gtgataacaa tacagttgac tataatagtg atggggtaat
     2041 taactcgata gataaaatta actgtttaca aagtaagaga cgctaggtag aataggcaat
     2101 tgttaaatta aggaggtgtt tttttaatgc caatcatagt agaaaaaaag aaaggcgagt
     2161 ctaaagacga tattatcggt aagtttaggc gtctttattt ggaggaagat atcactgagg
     2221 aagtgcgaaa gcgaacagcc tatgttaagc cctcagagga gagatatgcc aaaaagaaga
     2281 taattgtgtg gcgggataaa tgccgccgcc gggctaaaaa ttccagaaga cacgatgtta
     2341 aaagataaac ttaaaagaga tagcacagaa gctttaaaaa accacgatca aaagatggtg
     2401 gatactttga ggtttttgat ttctctgatt gataaaaaag aattatccct tcctgtggga
     2461 gaaatgacgg aggcagaaga ggtcagagtt ttgcgaaagg agctaaaaaa taaagaagaa
     2521 tcaagagaaa tgtttgttaa gggtggccgg aacgatttgg tggcgcagtt ggattatgaa
     2581 atagagatag ttaaatctta tctgccggca gagataacgg aggatcaaat taaaaaggtg
     2641 gtggacgaga taataggggg caaggaaaac aattttggtt tggtgatgaa agaagttatg
     2701 actaaattgg gtggtgaagt tgaaggatca acggtggcaa gaatagtaaa agaaaagttg
     2761 tctggcaatt aaatgacaaa caaagacgga attttgatca ggcataataa aaactggggg
     2821 ttgagtgagt ctttggtaaa aaaaatagcc aaaaaggttt taagggagcg aggatttggt
     2881 gatgaagtag agttgagtat ttactttgtg ggaagaaaga aagcaaaaga gctgaataaa
     2941 aattaccgaa gaatggatta tattcctcaa gtgttagggt ttcctatgtc gacaacccct
     3001 ggtgtagacg ataaagtcag attgggggat attgtgattt gtactcagaa attgaaatat
     3061 gaggttgagt ttcaaaaatc atctgtagaa agggtgatgg aagattggtt aagacatgga
     3121 ttggataatt tgttaaaaaa agattaaact tagtttaagt tggcaactca aaaaaatccg
     3181 atatctattt tggtaatgat gttatttttg tcgtcagctt tgttggcggc gatagggttg
     3241 gtggaaaaga atcaggaaaa taggagtctg gcggcggggt taaatagtag ctgcacggtg
     3301 ggtagcggct gtggttggga taattgtaga ggagactgtg ttgggtcagc tgatttagtt
     3361 tgttatggtt cttcgactag agtttgtaaa ataaaaagcg ggggaacctg tagtagatca
     3421 agtgattgtt atggcaacca ttattgcagt cggtataaca accattgccg taaaaaagat
     3481 aaaaagtgga ttggtgatta ttcagttgag ggggtaagtg ttagctcaat gttgggagta
     3541 tcgtcggggt caacgacggc gccaaaacct tcatcggtaa cacagccaac gtcagtgccg
     3601 cggtcaacaa ctttgccgac ggcaatacca acaaaaattc cgggaccaat taccttgccg
     3661 gcgggaatgg cctctccagg cataagaata gtgacaggat tggttagctt atattccgga
     3721 gagaaacaaa agatatcagt ggtattaaat ggagttgagg gtggtggttt ggtatggacg
     3781 agtggcaatc aaaatgtggt tagagttgat aatttaggtt atgccacagc tgtagctccg
     3841 ggggtaacta ccataaaggt gcaatcaggt aagagtgtgg cggtcgtgcc ggtatgggtt
     3901 agggaggttg ggtctttgag gagcgtgatt gtggtgccga cagtgatggt aactttggcg
     3961 ccagcggcgc cgacattaat tccgacattg gttccaacca gggtgccaac ggcgatttcg
     4021 acggcaatac caactttggt tccaactgta atcccgacgg gagtaccgac tttggtgcca
     4081 acgcttattc caacagtaat accaacgtta gtaccggatg gtgagttgac gccaaaaatt
     4141 agttttaggt ttagtttaac cgggataaaa ccggggtctg tttgtatcag tagtatcgga
     4201 cctttggagg tagaagtggt aaataggtca agcgggactt atcaaggggg tttggttgct
     4261 agatttaaac cggttgaggg ggagattgac agtttgggag atcaggtgtt tgaggtgtca
     4321 aatttgtttt tggataaatc taagtttggc ggagtagatg aaactaattt tttaaaaata
     4381 aaaggtccat ttcattcaaa aaacaagatg tgtcaggaag ggcagtcggg aaaattgtcg
     4441 gaaaatacag tgtgtaattt gaagctagac gggagtaagg tttacgattt ttctaactat
     4501 gctttggctc cgggggatgt gaattatgat ggggtgatta attcgataga ttattctcaa
     4561 acaaagaaga tgatgaatga atcagaaatg gaaaatgcgg tatgcggtat taagggagat
     4621 ttcaatcttg acggaatggt taattcggtg gatatgggac tgataaaaat gtcgctgagc
     4681 caaattgatg atgaatagag cgggttgata tagaaaatga tattgtaaat ccagcctggt
     4741 tttggtttaa tctattgatg tctgtgtttc acttaaaata ccggccgagg agatggtcgg
     4801 atttggattt ggctgatgta gctgataaat taaacaagat tttgtcggcc aaagacaagc
     4861 cgcagtcgtt tttgtttgcc ggaccaaagg gatcgggtaa aacttcggca gccagaattt
     4921 tggccaaaac ggtcaattgt ttgaaaccag aaaagggtga ggcgtgtaat agttgtgata
     4981 actgtgagga gattgataaa ggtcgggcga tagacataat tgagatggat ggggcttcta
     5041 ataggggaat tgaggatgtg agaaatttaa aagacaaagt ttacttgtcg cccagtaaat
     5101 tgaagtataa ggtgtttatt attgatgagg tgcatatgtt gaccaaggat gcgtttaacg
     5161 ccttgttgaa gctgattgag gagccgccaa agcataccat gtttgtgctg tgcaccacag
     5221 atgcagaaaa aattccggat acggtgatgt cacggttggt tcggattgat tttcggcggg
     5281 gcgggagaga ggaactgcgt cattctttgg ataaagtaat taagggagaa aaaatagtgt
     5341 tggacaaaac gggaattgat tttattttgg acaggagtga tggcagtttt agaaacctgc
     5401 aaagaagttt aaatgaaatt gtgcttaatt ttgggacgga attgacgttg acagaggtaa
     5461 aaaaattttt tttggacaaa ggaggcagtt ataatgaggc tgattttgag gctgatttga
     5521 ttttgggaaa ggcgggagta attttggaaa aactggaaaa aatggcagat gaaggacggg
     5581 atttgactga ttttaggcaa agattaatga agtattttca aaccaaagtt ttggctgttt
     5641 ttggagtggg agaagaaatt aaatcaaaaa tgtcagtaac cgagctgcaa aggtggatag
     5701 aacttctgat cagatgcgcc agacaggaaa aagagacgga gattgatcag ttgccgttac
     5761 aattggcggt gatagatttt ttggaggatg aaagcggcgg tgaggaagca aaaagagttg
     5821 agacagagat taatgatgag aaaaaaaata agacaaaggc agagccggcg ccaaaagcaa
     5881 gagcagtgga gaaaaaggaa gagagtaaaa cggaaattat aaacatagat gtgtcagggg
     5941 ttagcttgga aaaaatcagc cagttttggg ggaatgtgtt gatggcgatt aaaccgttta
     6001 atcattcagt agaggcgttt ttgagagctt cgagaccgat caagctggat ggacgacact
     6061 tggtagtaga agtgttttat ccgtttcaca aagacaggtt agaggagcca aaaaaccggc
     6121 agatagtaga aatggggtta caaaaggcgt tgggggtaga gctggtgttt gactgtattt
     6181 taagcaaaag caaaaagcag ccattggtga ttaaaaatga cacgccgata gaaagcgtaa
     6241 gtgaggagct ggtggaaagt aaaaaagata aggctgattt gtatgatgtg gcgaaggata
     6301 tttttgggta aaaagcagag agaaaatgat gggggacaaa aggagaaatt aataatataa
     6361 tggtgacagg ttattttttt tgaaaaatat gtttgataaa gcaaaaatgt tagctcaggc
     6421 ttataaattg aagaaagcgg tggaggcaga ggtagttgag attgaggaaa atggagttaa
     6481 ggtgagtgtc agcggggatc aaaaggttaa gtttttgtcg gttaatggga tggaaaataa
     6541 ggtgttggtg gatacaatca acaaagcgtt gaaaaaatcc caagagacag cggcaaagag
     6601 aatgaaggag atgggcggtt tggaggggtt attttagcaa gaggttgtaa attataagtt
     6661 tgagggctaa aagtggcgga atttcctaaa agtataaaaa atttaattga agcgtttgag
     6721 cggttgccgg ggattggtcc taagggagcg accaggttgg tattttattt gctaaatacc
     6781 cccaaggctt acgtgcagga tttggcaacg acgttgataa agatgaaaga tgaaattaaa
     6841 atttgtcggg aatgtttttc ggttactgaa caagatgttt gttcaatatg tagagataaa
     6901 cagcgaaact cgtggcaaat ttgtgtggta gaaagagcaa tagatgtgat ggcgattgag
     6961 aacatgggag gatataaggg ggtatatcat gtgttgggag gggtgattaa tccgttggaa
     7021 cacgtgggtc cagaggaact gaagattgat gagttggtgg agagagtcaa gaataaatta
     7081 aatggagagg gagattgtct ggaggtaatt ttggcaacta acccaacgat ggagggtgag
     7141 gcgacggctt tgtatataaa aaaaagattg gaggggttga aggtaaaaat atcccggatt
     7201 ggcagcggac tgccgattgg ggctgatttg gagtatgctg atcaggcgac tttgtgccgg
     7261 gcgctggatg gaagaagaga tatataatgt atatatgaca aaaaaatata ttgcggtagt
     7321 aataataatt gtagctttgg tggcggcggg gattttttgg gcgaatccaa aagataaaag
     7381 taagcagggg ttgacggaag ataaaattag ttcgacggag atgttggaaa taacaatgga
     7441 gccaacggag gtaaaaggaa aaatagaggg aaaggtgtgt tatccgtcga gttttattcc
     7501 cgaagggaaa a
//
LOCUS       DAOSHV010000019         7410 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_8652_length_7410_cov_5.501564, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000019 DAOSHV010000000
VERSION     DAOSHV010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 7410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7410
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8652_length_7410_cov_5.501564"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="PKZ29_01310"
     CDS             complement(<1..265)
                     /locus_tag="PKZ29_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37544.1"
                     /translation="MTNNDRNTRTLIVCFVIALMALVPMSIKEIGQENQNRVMVLGEE
                     TAGMDMETNLQEDEPIEEVETEVYTQAEEMPASEDVIEEVWDES"
     gene            352..425
                     /locus_tag="PKZ29_01315"
     tRNA            352..425
                     /locus_tag="PKZ29_01315"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:386..388,aa:Pro,seq:cgg)
     gene            589..660
                     /locus_tag="PKZ29_01320"
     tRNA            589..660
                     /locus_tag="PKZ29_01320"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:622..624,aa:Arg,seq:cct)
     gene            complement(1429..2664)
                     /locus_tag="PKZ29_01325"
     CDS             complement(1429..2664)
                     /locus_tag="PKZ29_01325"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOG37545.1"
                     /translation="MKTKISTLKNGIRSIIVPVEGLKSVTVEVFIKIGSKYELKDEFG
                     MSHFLEHMAFKGTVKRPTAAAINQEIDGKGAGYNAGTSHEMTSYHITTTKENIGWAVE
                     LLSDMLKNSIYEDKEVLKERGVIMEEIRMYRDNPMMGLSGEMTKFLYGGSTIGCWDIA
                     GEISDIEKVSRLRVVNFRNKLINPKEMVVVIAGNVDDSANDEVKKYFEDFENKFYKGL
                     PEVKVKINDKLVKSMTREVEQGHFAMALPALKRGDERKYAFKLIDIILGGNTSSRLYQ
                     KIREDMGLAYYVFSISDSFEEVGFWGVQSGVKLERLDEAVSVVRDELKHVSQGIKEEE
                     LNRAKDYMEGKTKLAMDRSSYWASFMGTKMLLENEIGDLDKELKKGREVSLVEVNNLV
                     KDVFKEEEIRLVTIKNSKK"
     gene            complement(2788..5262)
                     /gene="leuS"
                     /locus_tag="PKZ29_01330"
     CDS             complement(2788..5262)
                     /gene="leuS"
                     /locus_tag="PKZ29_01330"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016278950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HOG37546.1"
                     /translation="MDFRLIEDKWQKYWEEHPEIYEAGSEGKDKRDKKYVLVEFPYPS
                     GSGLHIGHAFSFTGGDIQARFWRMRGKKVLFPMGWDAFGLPTENYAIKTKKKPQAVTA
                     ENTQRFKEQMKKLAYSFDWSREIDTTDPAYYKWTQWIFIQLFKKGLAEKREMPINWCP
                     SCKIGLANEEVVDGKCERCGAEVSRRTISQWVVKITKYADKLIEGLKETDFIDKVKQS
                     QINWIGKSEGAKVRFKIQDLGFKNKILEVFTTRPDTLFGVTFMVIAPEHALVESLSSS
                     SSLSSEKKKEIETYVKNSRAKSDLERTELNKKKTGVFSGLEAINPVNNKKIPVWISDF
                     VLASYGTGAIMSVPAHDQRDFEFAKKFGLEVAPVMETKGKWDFNKEAYMGEGKMINSP
                     MIEGLESKEAIEKMIRWLEDEGIGETAASYHLRDWIFSRQHYWGEPIPMIYCPKCAHQ
                     NGGQGWVPVAEKDLPVVLPEVDHYEPTDDGKSPLSQMSHWVETECPKCGSKAQRETDT
                     MPNWAGSDWYFLRYVDPKNEKQLVDKNKWRYWGPVDIYIGGDEHNVLHLLYSRFIYLF
                     LRDMGAVVEEISEPYFRRISHGVILGPDGQRMSKSRGNVVVPETVAEKYGVDVARMYL
                     MFMGPFDATMAWNEKTLMGVKRFLERMDKFIRCQTNNDSVSNKEAKVIINKLIAGVTG
                     DLENFKYNTAIAKMMQAINEVSDVRCQMSEREIKTLIKLMAPVAPYVAEEWWSLVGGK
                     GSVHRAAWPEAEVKYLVDEETVVAVAVNGKVRSEIKIETKEVSNKNKIIDLAKKDKRI
                     KKWLSGGRIVKEIYVPGKMINFVVVS"
     gene            complement(5265..5564)
                     /locus_tag="PKZ29_01335"
     CDS             complement(5265..5564)
                     /locus_tag="PKZ29_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37547.1"
                     /translation="MINPEKADAIRQSLRQSGFGVADGVVKNIGNRDAEGKEIKKPSK
                     RKITQERYAVGTDKGITKGSFMSEMATLEVRTLVDEGEEGRQMEEKFLKDSGVAG"
     gene            complement(5582..6370)
                     /locus_tag="PKZ29_01340"
     CDS             complement(5582..6370)
                     /locus_tag="PKZ29_01340"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOG37548.1"
                     /translation="MKNKIIFLFSLAAMMIIFYATLPDSRGKVVFCDVGQGEAMLASY
                     KNIQILVDTGPNNKKLGECLARQLPFWDRTIETVILTHGDSDHIGGLEDLMKAYKVGK
                     FFSNGLLGKDVEQKIYSQKLRQNDVVSNNLFEFEVVSPGQNNIYEIDDNINSLAGVLR
                     YKPTGWSALLTGDIDVETEQRLVWRQILKGEIEALKISHHGSDSGTSEELLDWIKPTT
                     AIISVGKGNRFGHPREEVIEKLEKRKIEIKRTDEKGDIVFMMER"
     gene            complement(6367..7284)
                     /locus_tag="PKZ29_01345"
     CDS             complement(6367..7284)
                     /locus_tag="PKZ29_01345"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOG37549.1"
                     /translation="MDKNRGERWWWAGAVLTTLLMYKFIKRESLIGVVEQSLMMREAG
                     LLKGMLWGDKSGFGKDFYNQLKNSGLVHLVVVSGSNIMLVFRGMVEWGAKFLGRKKAI
                     GVGFLISLFYLEVVGWEIPVVRALILVGVMYWAQVLGRKYSLIRGLILSLMIIILAWP
                     GSVKEVSFWLSFLAFVGVATSPSRGALGLSLWVSLWISPVMGLVFGRINLVGPVSNLL
                     VLLTVEVVTVVGLVGSVVGLAVPILGKAILWSIWPLLKYFGKVVEMTGSWRWANIGVD
                     FNWMIGLGWYVILIWGLIKFRKGFDIKRK"
BASE COUNT         1993 a         2127 c          805 g         2485 t
ORIGIN      
        1 aactctcatc ccacacttcc tctattacat cttcacttgc cggcatttct tctgcctgtg
       61 tatatacctc ggtttccact tcctcaattg gctcatcctc ctgcaaattc gtctccatat
      121 ccatccccgc cgtttcttcc cccaatacca tcactcgatt ctgattttct tgcccaatct
      181 cttttatgct catcggcacc agcgccatta gcgctattac aaaacaaaca atcaaagtcc
      241 tagtatttct atcattatta gtcatacatt cagtatataa attcaaaact tttcgtcaag
      301 tcttaactta ttaacctact tataccaatt aagctataat agacatactt tcggggattg
      361 gcgcaattgg ctagcgcgcg cgcttcgggt gcgcgaggtt gcaggttcga atcctgtatc
      421 cccgaccgat acaggatgag aagtttatcc gccgtctggc ggaaagcctg tatccccgac
      481 cgatcagttt ataaagttgt aaagttaaaa gtttttaaag ataaatgcag ctacgttttt
      541 tgacttactt tacgaacttg ataaacttta aaactttcaa ctaattccgt cctcatagtt
      601 caacggatag aacggctgcc tcctaagcgg caaatctagg ttcaattcct agtggggaca
      661 ctcaaaagac aactctaaat agtttatccc cacaataagg tcttatttca tctcgtctta
      721 taatagcagt caaacatcag ctctgataac aatttttcat caaaatcatc acaatataac
      781 aataaaaatc aattaatatc aattaagctc ctccaattct ttagccttat ttttatcaaa
      841 aaatattttt ttattaactt ttttattatt ttttttacaa atttttctcc ccaaacatct
      901 attttttaat ttatctatct ccgacttcat ttttaactga tttatttaat taaccgcata
      961 ttttatacca gaaaaatatt aattaaccgc atatttttta tattctccgc atttttattg
     1021 acaataatta ttgtagcttt gagcaattta tttaccgaaa aaatattttt caaataaact
     1081 tactcaagta taacaattaa ctactataaa tatctttatt accgtaaata aacaataacg
     1141 cccaaaaaca cttcatttac aactccaaat ctggtaacta atacacattt catatgtata
     1201 taaaattctt cctttctacc cttaaagtac caaaattact atatcaatta aaatcatttt
     1261 ctcctctcta aatctcattt ttacaccctc aaatttcatc cataatcaaa gctaatcaaa
     1321 tttaaattac ccctaaaaca ccgatgctca tttgcaattt cttaatctag cagttaatac
     1381 ctttaattgc caaaacgtca ttattttgct ctgttataac atcccaacct attttttgct
     1441 attttttata gttactagtc taatttcttc ttctttaaaa acatctttta ccagattatt
     1501 cacttccact aaacttactt ctcttccttt ttttaattcc ttatccaaat caccaatttc
     1561 attttccaat agcattttag ttcccataaa acttgcccag tagctgcttc tgtccatggc
     1621 caacttagtt ttaccctcca tatagtcctt agctcgattt aattcctctt cttttatccc
     1681 ctgactcaca tgcttcagct cgtctctaac cactgatact gcttcatcca atctctccag
     1741 ttttaccccg gactgcactc cccaaaaacc tacttcctca aaactatcac taatagaaaa
     1801 aacataataa gccagtccca tatcttctct gatcttttga taaagtcttg atgaggtatt
     1861 gcctcccaaa attatatcta tcaatttaaa agcgtatttt ctctcatctc cccgtttcaa
     1921 cgccggcagt gccatggcaa aatggccctg ctccacttcc cgagtcatgc tttttaccag
     1981 cttatcatta atttttacct taacttcagg caatcccttg taaaacttat tttcaaaatc
     2041 ttcaaaatat tttttgacct catcattggc gctgtcgtca acgtttccgg ctatcactac
     2101 taccatctcc ttggggttaa tcaatttgtt tctaaaatta accactctca accggctaac
     2161 tttctcaata tctgatatct ccccggcaat atcccagcaa ccaatagttg accctccata
     2221 caaaaacttt gtcatttctc ccgacaaccc catcattgga ttgtcccgat acattcttat
     2281 ttcctccatt atcacccccc tctcctttag cacctcttta tcctcataaa tcgagttttt
     2341 cagcatatcc gacagcaatt ccactgccca accgatattt tccttagttg tcgtaatatg
     2401 atatgaagtc atttcatggc tggtgccggc attataaccg gctcccttac catcaatttc
     2461 ctggtttatc gccgccgccg tcggtctttt gactgtcccc ttaaacgcca tatgttccaa
     2521 aaaatgactc atcccaaact catctttcaa ctcatatttg gatcctattt taataaacac
     2581 ttctaccgtt accgatttta atccctcaac cggaactata atgcttctaa ttccattttt
     2641 taaggttgat attttagttt tcatcttttt tccggtcttt agaccaatct atttgtggtt
     2701 agcagttaat tataaattct gcttgatacc ttttgtagta ttcttttgag ttaattttgt
     2761 taactacatc attgtatcat tttgccttta actgactact acaaagttta tcatcttccc
     2821 tggcacatat atctctttaa ctatccgacc gccacttaac cattttttaa ttcttttatc
     2881 cttctttgcc agatcaatta ttttgttttt attactcacc tcttttgttt ctatttttat
     2941 ctcgcttctc accttaccat ttactgccac tgccaccacc gtctcttcat ccaccaaata
     3001 tttgacttct gcctctggcc aggctgctct atgaaccgaa cccttcccac ccaccaagct
     3061 ccaccactcc tctgccacat acggcgctac cggcgccatc agtttaatta aagttttaat
     3121 ttctctctct gacatctgac atctgacatc tgacacctca tttattgctt gcatcatttt
     3181 ggctatcgcc gtattgtatt taaagttctc cagatctcct gtcaccccag caattagctt
     3241 gtttattatc actttagctt ctttattgct aacactatca ttatttgtct gacatctgat
     3301 aaacttatcc attctttcca aaaatctttt aactcccatt aacgtctttt cgttccaagc
     3361 catcgttgca tcaaatggtc ccataaacat caaatacatt cttgccacat caaccccata
     3421 tttttctgcc actgtctccg gcaccaccac attcccccgc gacttgctca ttctctgtcc
     3481 gtccggaccc aaaatcactc catgagatat ccttctaaaa taaggttcac tgatttcctc
     3541 caccaccgcc cccatatctc tcaaaaacag ataaataaat cgggagtaaa gcaagtgcaa
     3601 cacattatgt tcatcccccc caatataaat atctaccgga ccccagtatc gccatttgtt
     3661 tttgtcaact aattgctttt cgttttttgg atcgacatat ctcaaaaaat accaatcgct
     3721 ccccgcccaa tttggcatcg tatccgtttc ccgttgggcc ttgctaccgc acttaggaca
     3781 ttctgtttcc acccaatggc tcatctgtga cagcggactc ttgccatcat cggttggttc
     3841 ataatgatct acctccggca gcaccaccgg caaatctttt tctgccaccg gcacccagcc
     3901 ttgcccgccg ttttggtgag cgcatttagg acagtaaatc atcggtatcg gctcccccca
     3961 ataatgttgt ctagaaaaaa tccaatcccg caagtgatat gacgctgccg tttccccaat
     4021 tccctcatcc tccaaccacc taatcatttt ttctatcgcc tcttttgatt ccaacccttc
     4081 aatcattggt gaattaatca ttttcccctc acccatataa gcctctttgt taaaatccca
     4141 ttttcccttt gtttccatca ccggcgccac ttccaatcca aatttcttgg caaattcaaa
     4201 atctctttgg tcatgagccg gcaccgacat aatcgccccc gtgccataag aagccagcac
     4261 aaaatcagaa atccaaaccg gtatcttttt gttgttaacc ggattaattg cctccaaccc
     4321 cgaaaaaact cccgtttttt tcttattcaa ctccgtcctt tccaaatcac ttttagccct
     4381 cgaatttttt acataagtct ctatttcctt cttcttttca cttgataaac ttgatgaact
     4441 tgacaaactt tcaactaatg catgttccgg cgctatcacc ataaacgtta ctccaaacaa
     4501 agtatccggt ctggtcgtaa atacctctaa tatcttattc ttaaatccta aatcttgaat
     4561 cttaaatctc actttcgccc cttcactttt tccaatccaa tttatttgcg actgttttac
     4621 cttgtcaata aaatcagttt ctttcaaccc ctcaatcaac ttatcagcat attttgttat
     4681 ctttactacc cattgagata tagtcctccg actaacttcc gctccacacc gctcacattt
     4741 gccatccacc acttcttcat ttgccaaccc gattttacaa ctcggacacc agtttattgg
     4801 catctccctt ttttccgcca accccttttt gaacagttga ataaaaatcc actgtgtcca
     4861 tttataataa gccggatcgg tagtgtctat ctcccgactc caatcaaatg aataagccaa
     4921 ttttttcatc tgttctttaa atctttgagt attttctgcc gtcaccgcct gaggcttttt
     4981 tttggtctta atcgcataat tttcagttgg cagaccaaaa gcatcccatc ccatcggaaa
     5041 cagcaccttc ttgcctctca ttcgccaaaa tctggcttga atatccccgc cggtaaacga
     5101 aaatgcatgc cctatatgca gtccgctgcc gctaggataa ggaaactcta ccaacacata
     5161 ttttttatct cttttatctt ttccctcgct tcccgcctca tatatctccg gatgctcttc
     5221 ccaatacttt tgccatttat cttcaatcag tctaaagtcc ataatcaccc cgctactccg
     5281 ctgtctttta aaaatttttc ttccatctgt cgcccctctt ccccctcgtc taccaacgtc
     5341 cttacctcca gtgtcgccat ctcgctcata aatgaacctt tagttatgcc tttatctgtt
     5401 cccaccgcat atctttcctg agttattttt cttttgctcg gtttttttat ttctttaccc
     5461 tcagcatccc gattaccgat atttttgacc accccatctg ccaccccaaa accgctctgt
     5521 ctcaaacttt gtctgatagc atctgctttt tccggattta tcatgaatca attttaccat
     5581 tttatctctc catcataaag acgatgtccc ctttctcatc tgtccttttt atttctattt
     5641 ttctcttttc caacttctct attacctcct ctcttggatg accaaatcgg ttacccttac
     5701 ccacactaat tatagccgtg gttggtttga tccaatccaa caattcctcg cttgtcccgc
     5761 tatcagatcc atgatgtgat atttttaatg cttctatttc tccctttaaa atctgccgcc
     5821 acaccagcct ttgctccgtt tccacatcaa tatctcccgt caacaacgcc gaccaacccg
     5881 tcggtttata tctcaatacc cccgccaatg aattaatatt gtcatcaatt tcataaatat
     5941 tattttgccc cggagacacc acctcaaatt caaacaaatt attgctaacc acatcatttt
     6001 gccttaattt ttgtgagtaa attttttgtt caacatcctt acccaacagc ccgttgctaa
     6061 aaaattttcc taccttataa gctttcatca aatcctccag acctccaata tgatcgctat
     6121 ccccatgagt caaaattacc gtctcaattg tcctgtccca aaacggcagc tgccgcgcca
     6181 aacactcgcc tagtttttta ttattcggac ccgtgtctac caatatctga atatttttgt
     6241 agcttgccag cattgcctcc ccctgaccca catcacaaaa caccaccttc ccccgactgt
     6301 ccggcaaagt ggcataaaaa attatcatca tcgccgccaa actaaacaaa aatataattt
     6361 tgtttttcat tttctcttaa tatcaaatcc ttttctaaat tttatcagcc cccaaatcaa
     6421 tatcacatac caccctaacc ctatcatcca attaaaatct acccctatat tcgcccatcg
     6481 ccaactcccc gtcatttcca ccacctttcc aaaatatttc aacagcggcc aaattgacca
     6541 taatattgcc tttcccaata tcggcactgc caaacccact actgatccca ccaaccccac
     6601 cactgttacc acttccactg tcaacaatac cagtaaattg ctcaccggtc ccaccaaatt
     6661 tattcttcca aacaccaatc ccatcaccgg gctaatccac aagctcaccc acaaactcaa
     6721 acccagtgcc ccccgactgg ggctggttgc cacccccaca aatgccaaaa aactcaacca
     6781 aaaactcact tccttcaccg accccggcca agccaaaatg attatcatca aactcaaaat
     6841 taaccctcta atcaggctat attttcttcc caatacctgc gcccaataca tcactcccac
     6901 caaaatcagc gccctaacca ccggtatttc ccaccccacc acttccagat aaaacaaact
     6961 tatcaaaaac cccaccccaa ttgctttttt tcttcccaaa aattttgctc cccactctac
     7021 catccctcta aacaccaaca ttatattgct ccccgacacc accaccaaat gcaccaaccc
     7081 gctatttttt aattgattat aaaaatcctt tccaaaaccg cttttgtctc cccacaacat
     7141 tcctttcagc aaccccgctt ccctcatcat caaactttgt tccaccaccc ctatcaaact
     7201 ctcccttttt ataaacttat acatcaataa tgtcgtcagc actgcccccg cccaccacca
     7261 tctttctccc cgatttttat ccatcttaat atcaatttac caaatccacc tcaccaaaac
     7321 ataagtccta gattttagtt ttagcgccga caatattttt gagattttta ggtctaaatt
     7381 tggaatcaaa tgacataatt ttgcctaaaa
//
LOCUS       DAOSHV010000020         7340 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_8770_length_7340_cov_4.480027, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000020 DAOSHV010000000
VERSION     DAOSHV010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 7340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7340
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8770_length_7340_cov_4.480027"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="PKZ29_01350"
     CDS             complement(<1..402)
                     /locus_tag="PKZ29_01350"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOG37550.1"
                     /translation="MVAILFFLFFILCSPALAAEPDIKITGYSASGSEYVELTNNTDQ
                     EINLAGWVIKDDNDIDDDIHLTSADTIPAKSPKKFVNTKDSTWLNDKGNTIYLYNNQN
                     QLVDSLKYPQSTPISTQTPTPNLTPTTVSPSP"
     gene            complement(419..1870)
                     /gene="gltX"
                     /locus_tag="PKZ29_01355"
     CDS             complement(419..1870)
                     /gene="gltX"
                     /locus_tag="PKZ29_01355"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOG37551.1"
                     /translation="MTEVSQKPARTRIAPSPTGELHIGTLRTLLYDYALARQTGGQFI
                     FRLEDTDQKRYVEGSETRLMEVIKKYGLDWDEGPDIGGPYGPYIQTERLDIYHQYVNQ
                     LLDKGLAYYCFCTQERLDDLRATQKAAKQVPRYDQHCLNLSQEEIKQKLAQKIPYTIR
                     MVIPKNETIICDDLIRGRIKFNTNDVDDQVLIKSNGIPTYHFAVVVDDHLMKITHAFR
                     GEEWLTSTPKHILLYRYFGWQPPIFAHLTVLLNADGKGKMSKRSGTTHAQAFLDEGYL
                     PEAVLNFLMLQGWNPGDDREFFTLDEFVAAFSLDRLHKKSPIFDPKKLNYFNGYYLRQ
                     KTDSQLLKLIKPFIPQSDDQTLIQLIPLLKDRISTLKNAADLTRFIFTDIDYSPDLFT
                     QKGIDLAEAKNFLTQTRDLLQSQSDWSFDHLQTKLLDLVKTNNWHTGHFFMVFRIAIC
                     GSPFTLPIVEVLPILGKDKTVRKIDIALNKLGQ"
     gene            1905..2942
                     /locus_tag="PKZ29_01360"
     CDS             1905..2942
                     /locus_tag="PKZ29_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37552.1"
                     /translation="MSLTQTAYTSRRLIKYGGIGVISFMILWAVGVAAYKAYIKAHPP
                     YVAPTVKYGLLQKIAFPAKTVEPKEFTFELPGDAVPTFSDQAKVYVIYRPNRAFLALE
                     EDKKTAASFGFADEPKEIKTGIYEFKNNNTNKTLTVNVLDGNFNMTYPYATDQLLQVP
                     EDMPNKAEATQLASAFLNKGGKMINDLKEGLTEVSFWKFDKDGLKSVLAQSEANAAKV
                     DFFRKDLEGMKLVSANFGQASVSVLVSGSRTEAKRIIEVNFKDVNIDRESYSTYPIKT
                     SEEAINSLKTGNYWTAKDVSNKNVVIRKMYLAYFEPTTLTNYLQPIFVFEGDNGFVAY
                     VPAINSSYISN"
     gene            complement(2927..3874)
                     /locus_tag="PKZ29_01365"
     CDS             complement(2927..3874)
                     /locus_tag="PKZ29_01365"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HOG37553.1"
                     /translation="MPPKPVNLKNNWLDFSIFVLILLLNLFWLPTFSFWNIYPSPFFS
                     SRNRQLLDFKSPPTPVIKMSSAPTVLADSYILVDVATNKILSSKNASQKIYPASTTKL
                     ATALTALNLYPLDEIVTVNQTYHEGKVMELKLGEKITVKSLVTALLVNSANDSAFTLA
                     DHYQHGSSIGFVEQMNLITNRYGLKHTNFINVDGIHHPNHFSTVYDLSQLGRISIKNP
                     TLRELVKIKDGLVSDVTNTIHHPISSTNELLGVTPEIIGLKTGWTPEAKGCFIGLLDI
                     NGHEVISVVAQTDDRFQDTKNLIDWVKNNVSWANHQFEI"
     gene            complement(3843..5624)
                     /gene="aspS"
                     /locus_tag="PKZ29_01370"
     CDS             complement(3843..5624)
                     /gene="aspS"
                     /locus_tag="PKZ29_01370"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF001750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOG37554.1"
                     /translation="MDRILAKECLQKIGEKVLLQGWVSNIRDHGQLIFIDFRDWSGII
                     QIVVDAKNNSQNHKIASLLGSEWIISVEGEVVNRSPKAINPNLPTGSIEINCQKLTLL
                     NKSDPVPFPLDTDGLDIDESLRLKYRYLDLRRPRLAKIIAAKHQYTLAVRNWMDKNGF
                     TEVITPLLTTTSPEGARDFLVPSRIHQGKFFVLPQAPQQFKQLLMISGVDKYFQIAPC
                     FRDEDPRADRHAGAFYQIDIEMSFPTIDKIFAVAENMISQTYASIAPQKKIAQVPFPR
                     LSYREAIDKYGSDKPDTRFGMELQDITSVIKNKSDFNVFNNAQAIKCLVAPKGGDFSK
                     TQIEKLENLAKEKGARGLAYTKVVSDGLDIGVARFFDSQLQQQIIKFTSAKVGDMIFF
                     IADSLSVANRVLGQIRLALGDILNLRDPNILKFNWITGFPFYEINDDGKLDFGHNPFS
                     MPKGGLEALKTQDPLEIESYQYDLTLNGFELASGSIRNHEPETLVKAFEVIGYGRQEV
                     IKKFGGLYHAFHYGAPPHGGWAIGLDRFLMLLLDEPNIRDIYAFPLNSNGFDLLMNAP
                     SQVDKKQLDDVGLILKSNAPKTRQSKK"
     gene            complement(5634..7259)
                     /gene="recJ"
                     /locus_tag="PKZ29_01375"
     CDS             complement(5634..7259)
                     /gene="recJ"
                     /locus_tag="PKZ29_01375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HOG37555.1"
                     /translation="MILIDRHTSSDDIIALLLKNRHISPKKISEFLSPRAPHQLEPKV
                     FGVASKQLDQAVAQIKQTVKNHQNVLIYGDYDVDGISATAILWQALYSQGAQVIPFVP
                     DRELDGYGIKSESFFRFQQQKNIHFDLLITVDNGIVAHDQLKPILDAGTQIIVIDHHA
                     AGPKLPLPIITVHSTKISGAALSWLVAQKIYPHADLGLAALGTVADCLPLIDINRNIV
                     FHGLQSLRLNPNFGIKKLISLSGIKPDSISTYDLGFVLGPRINAVGRLSNPTDALRLL
                     CSQNPVQASKFAQTLDDYNYQRQQIQQQSLEVADNLVQKNKNKLIFVADPSFHPGIIG
                     LIAGRLTEKYYLPSVIISQTDDISKGSCRSIKELNIIDTLREFSHLFISLGGHSGAAG
                     FSISTANIPQLKKNLTRFINRKLAGKTLKPTKFIDAEMKLNAVTVPNCRLLNQLEPFG
                     IDNPEPVFLFKQVKVVEKRLLGPAQNHLKLKVDDPTTSGKENILVDCIAFKKGDWSQK
                     INVGDLIDFTASLNLNSWNGQTFPQLVVKEILQ"
BASE COUNT         2283 a         1259 c         1732 g         2066 t
ORIGIN      
        1 aggagaagga gaaacggtgg taggggtgag attaggggta ggagtttggg ttgaaattgg
       61 tgtagactgg ggatatttta gactgtcgac aagctggttt tggttattat aaagataaat
      121 ggtattgcct ttgtcattta accaagtaga atctttggtg ttaacaaact tttttggtga
      181 tttggcggga atagtatcag cagaagtcag atgaatatca tcgtcaatat cgttatcatc
      241 ttttataacc cagccagcca gattgatttc ctggtcagta ttgttggtta gctcgacata
      301 ttcagagcca gaagcggaat atccagtgat tttaatatca ggttcggcgg caagggcggg
      361 agaacaaaga ataaaaaata aaaaaaataa tatagcgacc acaaactatt ctaatagatt
      421 attgacctaa tttgttaaga gcgatgtcaa tttttctgac agtcttgtct tttcccaaaa
      481 taggcaaaac ttcaacaatg gggagagtaa agggtgaacc gcaaatagca atccgaaaaa
      541 ccataaagaa atgaccagta tgccagttgt tagttttaac cagatcaaga agtttggttt
      601 ggaggtggtc aaacgaccaa tctgattgtg attgaagcag gtctctggtt tgagtgagaa
      661 aatttttggc ttcagcaaga tcaatccctt tttgggtaaa caagtcggga gaatagtcaa
      721 tgtcggtaaa aataaaccgg gtgagatcgg cagcattttt gagagtagag attcggtctt
      781 tgagaagagg aataagctgg ataagggttt gatcgtctga ctggggaata aaaggtttga
      841 ttagtttcaa aagttgggag tcagtttttt gacggagata gtaaccgttg aaataattta
      901 attttttggg gtcaaaaata ggagattttt tgtgcaagcg gtctaaacta aaagcggcga
      961 caaattcgtc gagagtaaaa aattcccgat cgtcgccggg gttccaacct tgaagcataa
     1021 gaaaatttag gacagcttcg ggcaaataac cttcatctaa aaaagcctgg gcatgggtgg
     1081 tgccggatct tttactcatt ttacccttac cgtcagcatt tagtagaact gtcagatggg
     1141 caaaaatcgg cggctgccaa ccaaaatagc gatacagcaa aatatgcttg ggagtagagg
     1201 taagccactc ttcgccgcgg aaagcgtggg taattttcat gagatgatca tcaaccacga
     1261 cggcaaaatg ataagtagga atgccgttgg atttgatcaa aacctgatcg tcaacgtcgt
     1321 tggtattaaa tttgatccgg ccgcggataa gatcgtcaca aataatagtt tcatttttgg
     1381 gaataaccat tctgatggtg tagggaattt tttgagccaa tttttgttta atttcttctt
     1441 gggataagtt gagacaatgt tgatcatagc ggggaacctg tttggcggct ttttgggtgg
     1501 cacggagatc atcaagccgc tcttgggtgc aaaagcaata gtaagccaaa cctttgtcca
     1561 aaagctggtt gacatattgg tgatagatgt ctaatctttc ggtttgaata tagggaccat
     1621 atggaccgcc aatatcagga ccttcgtccc aatcaagacc atattttttg ataacttcca
     1681 taagtctggt ttcggaacct tcaacatatc ttttttggtc agtgtcctca agacggaaga
     1741 taaattgacc gccggtttgg cgggcaaggg catagtcgta aagcagagtt ctgagagtgc
     1801 caatgtgaag ctctccggta ggactggggg caattcgggt tcgagcgggt ttttggctaa
     1861 cttcggtcat agcttatatt ttaccattta gtgtaaaata atttatgtcc ttaactcaaa
     1921 cagcctatac cagccggaga ttgataaaat acggcggaat tggggttatc agcttcatga
     1981 ttttgtgggc ggtaggagta gcggcttata aggcttatat caaagctcat ccaccatacg
     2041 tagcgccaac agttaaatat ggattgctgc aaaaaattgc ttttcccgcc aaaacagtag
     2101 aaccaaaaga gtttactttt gagctgccag gagacgccgt gccaacattt agcgatcagg
     2161 caaaagtcta tgtaatttac cggcctaata gggctttttt ggccctagaa gaggataaaa
     2221 aaacggcagc cagctttggc tttgctgatg agcctaaaga aattaaaacg ggtatttatg
     2281 aatttaaaaa taacaatact aataaaactt tgacggtaaa cgtgcttgat ggaaatttta
     2341 atatgactta tccatatgca acagatcaac tattacaagt gccagaagat atgccaaata
     2401 aggcggaagc aacacagtta gcctcagctt ttttgaacaa aggtggaaaa atgattaatg
     2461 atttaaagga gggtttgacg gaagtaagtt tttggaaatt tgacaaagat ggtcttaagt
     2521 cagttttggc tcagtctgag gcaaatgcgg ctaaagttga cttttttaga aaagatttgg
     2581 aagggatgaa gctagtttca gccaactttg gtcaagcatc ggtatcagta ttagtgtcag
     2641 ggtcaagaac agaagccaaa aggattattg aagtaaactt taaggacgta aatatagaca
     2701 gagaatctta ttcgacctac ccgattaaga ccagcgagga agcaattaat agcctaaaaa
     2761 caggaaatta ttggacggca aaggatgtca gtaataaaaa tgtggttatt agaaaaatgt
     2821 atttagctta ttttgaaccg acaacgctga caaattattt gcaaccgata tttgtatttg
     2881 agggtgataa tggctttgtg gcttatgttc cggcaataaa cagcagctat atttcaaatt
     2941 gatggtttgc ccaagaaaca ttatttttga cccaatcgat gagatttttg gtgtcctgaa
     3001 accgatcgtc agtttgagca acaacgctga taacttcgtg accattaata tcaagtaggc
     3061 caataaaaca acccttggct tcaggcgtcc aaccggtttt gagaccgata atttccggag
     3121 taactcccaa taactcattg gtagaagata tggggtgatg aatggtattg gtcacatcgg
     3181 agacaagacc gtcttttatt tttactaatt cgcggagagt aggatttttg atggaaatcc
     3241 gcccaagttg agacaaatcg tagacagtag aaaaatgatt gggatgatga ataccgtcga
     3301 cattgataaa attggtatgt ttgagaccat aacggttggt aataaggttc atttgttcaa
     3361 caaaaccgat actactgcca tgctgataat ggtcggccaa agtaaaagca gaatcattgg
     3421 cagaattaac caaaagggca gtaactaagg atttgacggt gattttttcc cccaatttaa
     3481 gctccataac cttgccctcg tgataggttt ggttgacagt aacaatttca tcaagaggat
     3541 aaagattgag agctgtaaga gcggtggcta atttggtggt agaagcagga taaatttttt
     3601 gagaagcatt ttttgacgag agaattttgt tggtagcgac atcaacaaga atataactgt
     3661 cagccaaaac ggtaggagcg gaagacattt taataacagg tgtcggtggc gatttgaaat
     3721 ccagcaattg gcggttgcgg gaagaaaaaa agggtgaagg atagatattc caaaaggaaa
     3781 aggtcggcag ccaaaataaa ttaagaagta aaatgagaac aaaaatagaa aaatcaagcc
     3841 aattattttt tagattgacg ggttttgggg gcatttgatt ttaggattaa accaacatca
     3901 tctaattgtt ttttgtcaac ctggctggga gcgttcataa gcaaatcaaa gccattggaa
     3961 ttaagaggga aagcataaat atctctgatg ttgggttcat ccagcaacaa cattaaaaac
     4021 cggtctaatc cgatagccca accgccgtgg ggaggagcgc cataatgaaa ggcatggtat
     4081 aaaccgccaa actttttgat gacttcctgc cgaccatagc cgataacttc aaaagccttg
     4141 accagagttt cgggttcatg attgcgaatt gaaccggagg ccagctcaaa accatttagg
     4201 gtaaggtcat attgataaga ttcaatttct agaggatctt gagttttgag agcttctagc
     4261 cctcctttag gcatagaaaa aggattgtga ccaaaatcca gtttgccatc atcatttatt
     4321 tcataaaaag gaaatccggt aatccagtta aatttaagaa tattagggtc gcgcagattt
     4381 aggatatcgc ctaaagccag acgaatttgt cccaaaaccc gattggcaac actaagactg
     4441 tcggcaataa aaaagatcat atcgccaact ttggctgagg tgaattttat aatctgctgt
     4501 tgcagttggg aatcaaaaaa cctagccacg ccgatatcta agccgtctga aacaactttg
     4561 gtgtaggcta agcctctggc acccttttct ttggccaaat tttcaagttt ttcaatttgg
     4621 gttttactaa aatcaccacc tttgggggca acaagacatt tgatggcttg ggcattgttg
     4681 aaaacattaa aatcactttt gtttttgatg actgaagtaa tatcctgaag ctccatacca
     4741 aatctagtgt caggtttgtc gctaccatat ttatcaatgg cttcgcggta gcttaaacga
     4801 ggaaaaggaa cttgggcaat ctttttttgg ggggcaattg aggcataagt ttggctgatc
     4861 atattttcag caacagcaaa aattttgtcg atggtaggga aactcatttc aatatcaatt
     4921 tgataaaaag cgccggcgtg acggtcagcc cgaggatctt cgtccctaaa acaaggagca
     4981 atctggaaat atttatcaac tccggaaatc ataagaagct gtttgaattg ctgcggagct
     5041 tggggtagaa caaagaattt accctgatga attctggatg gtaccaaaaa atctctggca
     5101 ccttcaggag aagtggtggt caaaagaggg gtgataactt cagtaaaacc atttttgtcc
     5161 atccaatttc tgacggcaag agtgtattgg tgtttggcgg caataatttt ggcaagccgg
     5221 ggacggcgca aatcaaggta gcgatatttt aggcggagag attcatcgat atctaaacca
     5281 tcagtatcca aaggaaaggg aaccgggtcg cttttgttaa gaagggttaa tttttgacag
     5341 ttaatttcga tgctgccagt gggcaggtta ggattgatgg cttttggact tcgattaaca
     5401 acctcaccct caacgctgat aatccattct gatcccaaca aagaagcaat cttgtgattt
     5461 tgagaattat ttttggcgtc aacaacaatc tgaattatgc cgctccaatc gcgaaaatca
     5521 ataaaaatca actgcccatg atcgcgaata ttgctaaccc atccttgtaa caagactttt
     5581 tctcctatct tttggagaca ttctttggct aatattctgt ccatgtgtgt attttactgt
     5641 agaatttctt taacgacaag ctggggaaaa gtctgcccgt tccaactatt aagatttaaa
     5701 ctagcggtaa aatcaattaa atcaccaacg tttatttttt gactccagtc cccttttttg
     5761 aaagcgatgc agtcaaccag aatattttct ttgccggaag tagtcgggtc atcaacttta
     5821 agcttgagat gattttgggc agggcctaaa agacgttttt cgacaacttt aacctgtttg
     5881 aacaaaaaaa ctggttcggg attgtcaatg ccaaatggtt ctaactgatt taaaagacgg
     5941 caattaggaa cagtaacggc gttgagtttc atctcagcgt cgatgaattt ggttggtttg
     6001 agagttttgc cggctaattt gcggttgata aaccgggtga gatttttttt aagctgagga
     6061 atgttggcag tagagatact aaaaccggca gcgccactgt gaccacccag gctaataaat
     6121 agatgagaaa attcacgcaa ggtatcaata atatttagtt ctttaataga acggcacgat
     6181 cctttggaaa tgtcatctgt ctgactgata atgacagagg ggagataata tttttcggtc
     6241 aaccggccgg caataagacc aataattccg ggatgaaatg aagggtcggc aacaaaaata
     6301 agtttatttt tatttttttg gactaaatta tcagccacct caaggctttg ctgctgaatt
     6361 tgctgacgtt gataattgta atcatctaaa gtttgggcaa atttgcttgc ctgaaccgga
     6421 ttttgactgc aaagcagacg gagagcgtca gtaggattgg aaagacggcc gacagcgttg
     6481 attctgggac caagaacaaa accaagatcg taagtagaaa tagagtcggg tttgatgccg
     6541 ctaaggctaa ttagtttttt gataccaaag ttgggattaa gccgaagtga ctggagaccg
     6601 tgaaaaacaa tgtttcgatt gatatcaatc aagggcaggc aatcggccac agttcccaga
     6661 gcggccaaac ccaaatcggc gtggggataa attttttgag ccaccaacca agagagagcc
     6721 gcgccggaaa tcttggtaga gtgaaccgta atgatgggca aaggaagttt ggggccggcg
     6781 gcatgatggt caatgacaat aatttgagtg ccagcatcta agatgggttt gagttgatcg
     6841 tgggcgacaa tgccgttgtc aacggtgata agaaggtcaa aatggatatt tttttgttgt
     6901 tgaaaccgga aaaaagactc ggatttgata ccataaccat ccagttctct atcggggaca
     6961 aagggaataa cttgcgcccc ttgagaataa agcgcttgcc acaaaatggc agtggcggaa
     7021 atgccatcaa catcataatc gccgtaaata aggacattct gatggttttt aactgtctgc
     7081 ttgatttggg cgacggcttg gtcaagctgt tttgaagcga ctccaaaaac tttaggttca
     7141 agctgatgag gagcgcgggg agacaaaaat tcggaaattt ttttggggct gatatggcgg
     7201 tttttgagca aaagggcgat aatatcgtct gaggaggtgt gacggtcaat aagtatcata
     7261 gataggtaat tttacacatt ttggcaaaaa tgttatatac tattggttag tttccgaagg
     7321 tggccatctt ttggagatga
//
LOCUS       DAOSHV010000021         7173 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_9077_length_7173_cov_6.360073, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000021 DAOSHV010000000
VERSION     DAOSHV010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 7173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7173
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9077_length_7173_cov_6.360073"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            299..451
                     /locus_tag="PKZ29_01380"
     CDS             299..451
                     /locus_tag="PKZ29_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37556.1"
                     /translation="MRKELGVKTPFQAVEKPLVRKAEPWFMLKYEIFIQNACIFKNKI
                     VISTTE"
     gene            832..4080
                     /locus_tag="PKZ29_01385"
     CDS             832..4080
                     /locus_tag="PKZ29_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019037451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOG37557.1"
                     /translation="MTIEQYLDIIDKRYKLGNATEHTFRGDLQQLIESIVPTIQATNE
                     PKRQSCGAPDYILTKRDASSRSGGIPVGFIEAKDIGDKDLDGEKKTGHKEQFDRYKAS
                     LNNLIFTDYIRFHLYHDGVLITKIEIGEITDKGIKPRPENFARFENLIKNFCIHIGQN
                     IKSPKKLAEMMAGKARLLSEIIERALISDEDNAENSSLKDQMNAFKEILIHDITPKGF
                     ADVYAQTIAYGMFAARLHDTTLNNFSRQEAAELIPKSNPFLRKFFGYISGPDIDDRIR
                     WIVDSLADIFLATNVEQILQNYGKATKMEDPIIHFYETFLSEYDPKLRKARGVWYTPQ
                     PVVSFIVRAVDDILKTEFDLPQGLADTSKTTIKVDMQGKKADQEVHRVQILDPATGTG
                     TFLAEVVKHIHQRFAGQQGIWSNYVETHLLPRLNGFELLMASYAMAHLKLDLLLKETG
                     YRPTRDQRFRVFLTNSLEEFHPDTGTLWANWLSTEASEANRIKKDTPVMCIIGNPPYS
                     GESANKGQWIMNLMEDYKKEPGGKEKLKERNPKWINDDYVKFLRYGQHFIEKNGSGVL
                     AFINPHGFLDNPTFRGMRWNLLKTYDKIYTIDLHGNSKKKETAPDGSADINVFDIQQG
                     VSINLLIKTGKKKAGELGQVFHFDLYGKRDFKYDFLTENSFSAINYKELPNVAPNYFF
                     VSKDFEAQKGYNNGFSIPELFLLNNVGIVTARDNFTIHTTKAEVKSTIDTFLSLDDES
                     ARARFNLGKDVRDWQVNLARKDLENHYPQKGVFSKISYRPFDDRWTFFTGKSKGFHCY
                     PRTEVMQHFKKNNVGLIIGRQGQVVGSMQWNLAFITKSITDFNLYYRGGGTVFPLYIY
                     PETNAQQTIEQETARKPNLNPEIVNQIANQLGLPFSPEKNPTASEPVELAPIDILDYI
                     YAVLHSPTYREKYKEFLKIDFPRVPYPKNKATFWQLVKLGGELRQIHLLESPLVEKCI
                     TQYPNNGDNIVKMVAFRTYDYDNTIPDENGNYNYPDYLGAVYINENQFFADVPTSVWE
                     LYIGGYKPAQKWLKDRKGRQLTFEDILHYQKIIVALSETHRLMKDIDLVNFM"
     gene            4203..5051
                     /locus_tag="PKZ29_01390"
     CDS             4203..5051
                     /locus_tag="PKZ29_01390"
                     /inference="COORDINATES: protein motif:HMM:NF022633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2971 domain-containing protein"
                     /protein_id="HOG37558.1"
                     /translation="MQPETKNNPNILFHYTSIGTLLDILSKQNEDNKICFRATHAKFL
                     NDPSEYQLAVSLLKQSMINYENENSIKNGNSTYFNDELIKKLTLVSGNPFILSFSANA
                     DDLSMWRSYGADGKGVAIGLDRKMLEAYANNDNTINTRLLQCIYNKQKIINKLTTEWG
                     NLYNEITFMDEGKKIGLSSFDFFFDIIKQSFSFKEEDYCVENEWRLCTNVTDNKTLRY
                     RNNGDLIVPYIEHYFNKEIIKKITVGPCVNKELSKESIEMFVKSKEYQLSSDSIIASK
                     ISYRQI"
     gene            complement(5790..6866)
                     /locus_tag="PKZ29_01395"
     CDS             complement(5790..6866)
                     /locus_tag="PKZ29_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002642560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOG37559.1"
                     /translation="MKNGNWKLAELPLNSDVESKRVLKSLPSAHASLAELKGIASTIP
                     NQTILLNTLGLQEAKDSSAIENIITTHDDLYKSELNLENFNSLNAKEVQNYISALKKG
                     FELIKKNGILTTRVILEIQEELEKNKAGFRKLPGTALKNSLTGETIYTPPQDYEEIKR
                     LMTNLERFINENEICDYDPLVKMAIIHYQFESIHPFYDGNGRTGRIINILYLIIQGLQ
                     NLPILYLSSYIIKNKSDYYRLLQEVREKNSWEEWLLFMINGVEITSKETISLIIQIRD
                     LMMQFKHTLRNNYKFYSQDLLNNLFKHPYTKIEFIVRDLNVSRITAANYLNKLSEDGL
                     LRKEKLGTGNYYINQPLFELLTKR"
BASE COUNT         2260 a         1555 c         1349 g         2009 t
ORIGIN      
        1 aacataattt tcaaaaagta ccgcagcagc taggtgctta aaacccaaag caaacctccg
       61 ctgccgcacg gttcctgtcg tgtggcaatc acctctccat ctctaaacaa cacttactac
      121 tccaatcctt aagtttacag cacataatcc tgccggatta tctttttact tatttctaac
      181 cacttactgt ccctttcatt ctttcactac cacacgagag agctagctac gttgagctat
      241 ccgccggctg gcggaacggt tggtgcggta gcgggggatt tataaaaaac atggcggagt
      301 gagaaaagaa ctgggcgtaa aaaccccttt tcaggccgtc gaaaaacctt tggtgagaaa
      361 agccgaacca tggtttatgt taaaatatga aatttttatc caaaatgctt gcatttttaa
      421 aaataaaatt gttatttcta caacagaata aaagtccaaa ttcttatcaa caaccttgtg
      481 aaacttgaca ttttatttac gaatgttgta tatttgttgc gtaaataaga cgttgtatgc
      541 aagtgttaaa ccccaaaatg atacgttaaa aagcaataaa ttattaacaa aaatatgaac
      601 tgaaaaatta ggttaacctg aagaaagaaa atgctgacag atacgaagaa atttttattt
      661 ttctcccccc gcacaaagag aagaaatcag ggaaccccac cgcacaaaca gcacatcccg
      721 caaaaacgca caggcaaacg caccaatcca attttgcggg aagagttgtt ttttgcccac
      781 gcaccgcaga aaatttgata catttgtact cctttgaaac gtttttgaaa aatgacaatt
      841 gaacaatacc tcgacattat cgacaaacgc tacaagctgg gcaatgccac cgagcacaca
      901 ttccgtggcg atttgcagca gctcatcgaa tccatcgtgc caaccattca ggcaaccaac
      961 gagcccaaga ggcaaagctg tggtgcgccc gactacatcc taaccaaaag ggatgcatcg
     1021 tcccgctcgg gaggaatccc cgtgggcttc atcgaggcca aggatatcgg cgacaaagac
     1081 cttgacggcg aaaaaaagac agggcacaag gagcagttcg acaggtacaa agcatccctg
     1141 aacaacctta tctttaccga ttacatccga tttcaccttt accacgatgg cgtgctgatt
     1201 accaaaattg aaataggtga gataactgat aagggtataa agccccgccc cgagaacttt
     1261 gcccgcttcg agaacctcat taaaaacttc tgcatccaca tcggccagaa catcaaaagc
     1321 cctaagaaat tggccgagat gatggccggc aaagcccgcc tgctctccga gatcattgag
     1381 cgagcgctca tcagcgacga ggataacgcc gagaacagct ccctcaagga tcagatgaac
     1441 gccttcaagg agatcctaat ccacgatatc acccccaagg gttttgccga tgtgtacgcc
     1501 caaaccattg cctacgggat gttcgccgcc cgtttgcacg acaccacttt aaacaacttc
     1561 agccgtcagg aggctgccga gctcataccc aagtccaacc cattcctgcg caagttcttc
     1621 ggctacatat cgggccccga tatcgacgac cgcatccgct ggattgtcga tagcctagcc
     1681 gatatcttcc ttgcaaccaa cgtggagcag atactccaaa actacggcaa ggctaccaag
     1741 atggaagacc ccatcatcca tttctacgaa accttcctga gcgagtacga ccccaagctc
     1801 cgcaaagccc gtggcgtatg gtacaccccc cagcccgtgg tcagcttcat cgttcgggca
     1861 gtcgacgaca tcctcaaaac cgagttcgat ttaccccaag gtcttgccga cacctccaaa
     1921 acaaccatta aggtcgatat gcagggcaaa aaggccgacc aagaggtgca tcgggtgcaa
     1981 atcctcgacc ccgccacagg cacgggcact ttccttgccg aggtggttaa gcacatccac
     2041 caacggtttg cggggcaaca gggcatctgg agcaattatg tcgaaacgca cctactgccc
     2101 cgcctcaatg ggtttgagct actcatggcc agctacgcca tggcacattt aaaactcgac
     2161 ttgctgctca aggaaacagg ctaccgcccc acccgcgacc agcgattcag ggttttcctc
     2221 accaacagcc tcgaggagtt tcaccccgac acgggcaccc tgtgggcaaa ctggctcagc
     2281 accgaggcca gcgaggccaa ccgcattaaa aaggataccc ccgtgatgtg cataatcggc
     2341 aatcccccct acagcggcga gagcgccaac aaggggcagt ggataatgaa cctcatggag
     2401 gattacaaaa aagagccagg cggaaaggaa aagctgaaag aacgaaaccc aaaatggata
     2461 aatgatgatt acgtaaaatt tttacggtac ggtcagcact ttatagaaaa aaacggaagc
     2521 ggtgttttag catttatcaa tccccacgga tttttagata acccaacttt caggggaatg
     2581 cgctggaacc tgctcaaaac ctacgataag atttacacca tcgatttaca cggaaatagc
     2641 aaaaaaaagg aaacggcacc agacggaagt gcagacatta atgttttcga cattcaacaa
     2701 ggggtttcca tcaatctgct tattaaaact ggcaagaaaa aggctggcga gcttggacaa
     2761 gttttccatt tcgatttata tggcaaaaga gacttcaagt atgattttct caccgagaac
     2821 tctttttcag ccatcaatta taaggagctg cccaacgtag cgcctaacta cttttttgtt
     2881 agcaaagatt ttgaggcaca gaaaggatat aacaatggat tctctattcc tgagctattc
     2941 ctactaaata atgtaggtat tgtaaccgct agagataatt ttacaatcca cacaacaaaa
     3001 gccgaagtta agtcaaccat tgatactttt ctcagcttag atgatgagtc tgctagagct
     3061 agattcaatc tgggtaaaga tgtaagggat tggcaggtaa atttggcaag aaaagattta
     3121 gaaaatcatt acccacaaaa aggggtcttt tcaaaaatct cttaccgccc atttgatgat
     3181 aggtggacat tctttactgg caaatccaaa gggtttcatt gttaccctag aactgaagta
     3241 atgcagcatt tcaaaaaaaa taatgttgga cttataattg gtcgacaggg acaagttgtt
     3301 ggttcaatgc aatggaattt agcatttatt actaaaagca ttactgactt taatctatac
     3361 tatcgtggtg gaggtacagt gttccccctt tatatctacc ccgaaaccaa cgcccagcaa
     3421 accattgaac aagaaacagc aagaaaacca aacctaaacc cagaaattgt aaatcaaata
     3481 gcgaaccagc ttggcttacc cttctctccc gagaagaacc ccacggctag tgagcctgtc
     3541 gagctggccc ccatcgacat tttagattac atctacgccg ttttacattc gccaacctac
     3601 cgcgagaagt acaaagagtt tctaaaaatc gattttccca gagtacccta ccccaaaaac
     3661 aaagctacct tttggcagct ggtaaagctg gggggcgagc tacgccaaat tcacttgctc
     3721 gaaagcccat tagttgaaaa gtgtataact caatatccta ataatgggga taacattgta
     3781 aaaatggttg ctttccgaac atatgattat gataatacta ttccagatga aaacggcaac
     3841 tataattatc cagattatct aggggcagtt tacattaacg aaaatcaatt ctttgctgat
     3901 gtccccactt ccgtttggga attatatatt ggcggctata aacccgccca aaaatggctc
     3961 aaagaccgca agggtcgcca gctcaccttc gaggatattc tgcactatca gaaaataatt
     4021 gtagcccttt cggaaaccca cagattgatg aaggacattg atttggttaa ttttatgtaa
     4081 aaacaaattt acttgcgtta tatactagaa ttttcgctgt ttttaaaact caacagcaac
     4141 ctctttagat taatatacat ctcattcgct taagttatta tccattgaat aaaacaataa
     4201 gtatgcagcc tgaaactaaa aacaacccaa acattttatt tcattatact tctattggta
     4261 cattattaga tatccttagc aagcaaaatg aagacaataa aatttgtttt agagcaactc
     4321 atgcgaaatt tctcaatgac ccatccgaat accaattagc agtttcactt ctaaaacaat
     4381 caatgataaa ttacgaaaat gaaaacagta taaaaaacgg caatagcacc tattttaatg
     4441 atgaactaat caagaaactg acccttgttt ctggcaatcc atttatactc tcgttttcag
     4501 caaatgctga tgacctttcc atgtggaggt catatggtgc tgacggcaaa ggtgtggcaa
     4561 taggtcttga taggaagatg cttgaagcct atgctaataa cgacaatact ataaacacaa
     4621 gattattaca atgtatatac aataagcaga aaattataaa taagcttaca acggagtggg
     4681 gcaatttata taatgaaatc acttttatgg atgaagggaa aaaaattggt ttatcttctt
     4741 tcgacttctt tttcgatatt attaagcaat cctttagttt taaggaagaa gattattgtg
     4801 ttgaaaacga atggcgttta tgtactaatg tcacagacaa taaaacttta agatatcgta
     4861 ataatggtga tttgattgtt ccatatattg agcattactt taataaagaa ataataaaaa
     4921 aaataacagt tggaccttgt gtaaataaag aactttctaa ggaatccatt gaaatgtttg
     4981 taaaatcaaa agagtatcaa ttatcaagcg actcaataat agcatcaaaa atatcttacc
     5041 gccagatttg aaaacagtga atataacctc tatgttttca cgctcttagt ttgcgatgca
     5101 tacaccctag tactgaacgc ttattgcaat ctaataatag tataattatg ctaaatttca
     5161 ttacaaagcg aattggagtt ttcctaataa ttagtatagt tttcaccgtt tttagtcctt
     5221 ggctatttac aagaaatctg ggtttaatag actttcatag tacgggtgat attggttcta
     5281 caattggtgg aattactgct cctatcacta gtctaatcgg tgcattatta atttattgtg
     5341 cgctagatgc acagaaaaaa gcgaataata ttcaaagtga aaacaatgca acacagacac
     5401 tcctatcgtt aattcttgac ttagaaaaaa aatgttatga tttagtcttt atagccgaga
     5461 gtaatcatca cagaacaggt attgcagcat tcaactcaat tagcagcata tttttttata
     5521 ggggaattag tcctccgctg ccgcacgatt ggcttcgccg agctcgcaaa aaacgactcg
     5581 gttgcctacg gcgggctatc cgccggctgg cggaacggtt ggtgaggtac ccgggctgcc
     5641 cacaacataa cggctgggcg tgtacccagt gggggagttc ggggccagcg tccttatccc
     5701 tcgataagga cgcccaatgc gggcgaaaca gttggattgt ctagaaatcc ccacattggg
     5761 tacacccctt gttaccgcct gtgctttaat catcttttag ttaaaagttc aaacaatggt
     5821 tgattgatgt aataatttcc tgtacctaac ttttcttttc ttaataaacc atcttctgaa
     5881 agtttattca agtaattagc tgcagtaatt cttgaaacgt ttaaatccct tacaatgaat
     5941 tcaatctttg tgtaaggatg tttaaataaa ttgtttaata ggtcttgact gtaaaactta
     6001 taattatttc ttaaggtatg cttgaattgc atcattaaat cgcgaatttg gataattaac
     6061 gaaatagttt cttttgaagt aatttcaacc ccattaatca taaataacaa ccattcttcc
     6121 cagctgttct tttctctaac ttcttgtaaa agtctatagt aatccgattt gttcttaatt
     6181 atgtagctac ttagatatag aattggaaga ttttgtagtc cttgaataat gagatacaag
     6241 atgtttatta ttcttcctgt tcttccattg ccatcataaa atggatggat gctttcaaat
     6301 tgatagtgaa taatagccat ttttaccaaa gggtcataat cacaaatctc attctcgttg
     6361 ataaaccttt caaggtttgt catcagtctt tttatctctt catagtcttg agggggagta
     6421 taaattgttt cgcctgttaa ggaatttttc agtgcagttc ctggcagttt tcggaaccct
     6481 gctttattct tctcaagttc ttcttggatt tcaaggatta ctcttgtggt cagaattcca
     6541 tttttcttaa ttaactcaaa cccttttttc agagcagaga tataattctg aacttctttg
     6601 gcatttaacg aattaaaatt ctcaagattt aattcggatt tatataagtc gtcgtgagta
     6661 gttataatgt tttcgattgc agaactatct ttggcttctt gcaatccaag ggtgtttaat
     6721 agaattgttt gattagggat tgttgaagca atgcctttta attctgccaa agaggcgtgt
     6781 gctgatggta atgattttaa aactcgttta ctctcaacat cactatttaa tggcaattca
     6841 gccaatttcc aatttccgtt tttcattatg taaagatttt gtacatttct ttacaaatat
     6901 aggttttttt gtaaaggaaa tcaaatattc ttaacatatc attccttctt tgtatgtttt
     6961 ttctgatttt attatcagat tctccttttt ctcatagcat tggcggtaac attcaaatag
     7021 taccatagca gccaggtgct taaaacccaa agcatacacc agccctaagc tgtgcctgct
     7081 tgcccgccat gaaattgggg gtgtaggttc caaccctatc ggtaatatcc gaaacaccgc
     7141 ctgaaaggta gatatagccg tcattaatca cct
//
LOCUS       DAOSHV010000022         7066 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_9265_length_7066_cov_7.876195, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000022 DAOSHV010000000
VERSION     DAOSHV010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 7066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7066
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9265_length_7066_cov_7.876195"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2021
                     /locus_tag="PKZ29_01400"
     CDS             <1..2021
                     /locus_tag="PKZ29_01400"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOG37560.1"
                     /translation="LSVEFIDKLYAGGIIKGGKWIKRDGAPSDTAVLFGRIIDRSLRP
                     LFPQGFLNEIQIITTVLSNDKNHDVVIPAFTAAAAALAISDIPFLGPVSAIRVGYVDD
                     KLVINPSLDQLQQSLFDLLVCTGPKGVNMIEADGKIVKNDLMLQAIELAKTTGDEVNS
                     QIAQFAAKAGKSKITFEPAGPSADLIKDVESKIKTDIKKFFDDGVDGAHMIGQEMIIE
                     KVKQLYQDKIDSEEVSLNHLVAAVDAILKNHVRQQTLSGHRYDRRQLDEIRPLNVEAG
                     VLPTTHGSALFERGLTQAITVTTLAPLSEKQYLEDSNGEVTKRYIHYYSAMPFSTGQT
                     GRVGRPGRREIGHGALAEKALVPVIPTEAEFPYTIVLTSEVLSQNGSSSMASTCGSTM
                     SLMDAGVPIKDKVAGISIGLMAETDDKYILLTDIAGIEDHFGDMDFKITGTRDGITAI
                     QLDIKREGLTLQMIRDTFAASTTARLQILDKMDSVLTAPRPELSPLAPKIKLVQLPED
                     KIGEVIGSGGKTIKALMDKYQVQIDIDDFGAASISSQNQKSIDDAAYEIESMIKEVQI
                     GEEYSGIVTRVENYGAFVEFLPGREALLHVSEMSGGFLSDPSTIIKVGDKLNVKIAGF
                     NDNHQIKLSSPEFKAAHPGQPRENNNRAQFAPKPEFRKFNPNFSKRPR"
     gene            2064..3125
                     /locus_tag="PKZ29_01405"
     CDS             2064..3125
                     /locus_tag="PKZ29_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOG37561.1"
                     /translation="MDSSVLTTAQAIETLRQKVNSVKLPDNLLTNLEELFSQLAINSR
                     NEESFWQNYQSASKYINYLISVPWFSQTQDILDTQFAREKLNEQHYGLDTVKDRLLEY
                     ISVLALQKDRNPDNPIKAPVLLFVGLVGTGKTTMAKSIASVLGRQLIRIPFGGLGDPL
                     YLRGQSRIHPEAEPGQVIKGLINSKSINPVILLDEIDRVADDALNTIMGVLVELLDPE
                     QNSHFVDHYIDYPVDLSRAIFCATCNNTSRIATAVMDRLEVIQMPSYTDEEKIVIGRD
                     YIFPKVIKEAGLRPEEIKIDASVWDQIVRPLGFDAGVRTLQRNIEGLCRKVAKMIFEG
                     KTKQVVVDESNVKYYLPQW"
     gene            3142..4899
                     /locus_tag="PKZ29_01410"
     CDS             3142..4899
                     /locus_tag="PKZ29_01410"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HOG37562.1"
                     /translation="MIKNKKNIKPRVNFSGAQNQVREFKSNQNSVRISSLGGFGDVTQ
                     NMFVYEFIPNGDTSKSQIIIVDCGVGFPEEDIFGVDMQIPDTHYLEDKKDRILGIFIT
                     HGHEDHIGAVKFILPILGEKIPIFAPKLAAAFIESRLSEVNQRAIINVYEDSIPVKRG
                     VFSVDPVRVTHSIPDTFHLSITTPIGIFYHGSDFKFDVFPLDGKRADLSHIARIGEKN
                     VVALMSDSLGSDHEGYSPSESSIAENFKYEITSAKGRVFVTSISSNIIRWSQAIEYAK
                     SVGRKIVIVGFSVDKAIGIAKDLSYLSLNDSDIVPPERAKNFPDNKLIFLVAGFAGQP
                     DSALSKMVMGKHRIKIKSGDKVIFSSPDYIPGTTSNIYTMIDNLSKLGAEVVYGEKEE
                     LHVSGHGYQKEHALLISLVKPKYLLPIGGNFRHVKQYQSMAKKLGYAEENVILPDFDA
                     SVTFYDNGKFDTNFHIPIRKILIDGFGIGDVGTTVLRDRKLLAGDGMFSVVILINKAS
                     GQMEKDPVVLSRGFVFVKENTDLINYLKQETIKKFNEATSSPANLDYIREEIQAHIEE
                     IIHQKTGRQPMVLPLVIEV"
     gene            complement(4901..5344)
                     /locus_tag="PKZ29_01415"
     CDS             complement(4901..5344)
                     /locus_tag="PKZ29_01415"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013386244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HOG37563.1"
                     /translation="MINKNNCFVCQRIELIKQNKNQYFVRELKSGYVVLADYQYFYGY
                     TIFISKIHIDELYKLGKTQKLFLEEMAKVAEAVIKTFKPKKLNYELLGNTDSHLHWHL
                     IPRYRNDPKPETAIWAIEKEIRCDERYKPSEKDLEKMKKKLIKNL"
     gene            5384..6610
                     /locus_tag="PKZ29_01420"
     CDS             5384..6610
                     /locus_tag="PKZ29_01420"
                     /inference="COORDINATES: protein motif:HMM:NF012796.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HOG37564.1"
                     /translation="MRYSQLFGKTSKSAPADADSINAKLLTQGGFIQKEISGVYNYLP
                     LGLRVLTKIQNIIREEMNQIGGQEILMPALTTEDSWVTTGRSHMDVLFHLKGQGNTNL
                     VLNPTHEEVVTPLAQKYIFSYRDLPFSVYQIQNKFRNEPRAKSGLLRGREFNMKDLYS
                     FHVDQTDLDKYYEVVKQAYFKIYNRIGLGDITVLTFASGGTFSQFSHEFQTINTNGED
                     TIYLCEKCQVAVNKEIIDVQKTCPECGSADLKTVKSTEVGNIFKLKTKYSQAFNFVYS
                     DQNGQEKIVEMGCYGLGPSRTMGTVVEVFHDDKGIIWPQSIAPYQIHLIALDLFEDNL
                     KQEADNLYQHLLDKNIEVLYDDRPDVAAGAKFADADLIGIPTRLVVSKRSLGAGGVES
                     KDRNQSQTKIIAIDSL"
     gene            6648..>7066
                     /locus_tag="PKZ29_01425"
     CDS             6648..>7066
                     /locus_tag="PKZ29_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37565.1"
                     /translation="MAKRGRKKKKIIPSLRLKPETIQTIFFIFFLILTILTVFSFLQT
                     GPIPARINQIIVKYFGLGSVLIPFILFLAAFLFAKIKTPIKDLNVFLGFLIMFISLIS
                     LFQHGIVGAFLFDQLVSLFGRVPSILIFIFCLIVGFV"
BASE COUNT         1943 a         1579 c         1324 g         2220 t
ORIGIN      
        1 ccttatctgt cgaatttatt gacaaacttt atgccggcgg cattatcaaa ggcggcaaat
       61 ggatcaaacg cgatggcgcc ccgtccgaca ctgccgttct ctttggtcgg attatcgacc
      121 ggtctctccg ccccctgttt cctcaaggat ttcttaacga aattcaaatt attaccaccg
      181 tcttaagcaa tgacaaaaat cacgatgtgg ttattccagc ctttactgct gctgctgcag
      241 ccttagctat ttccgacatt ccttttttgg gtccggtttc tgccatcaga gtcggttatg
      301 ttgacgataa attagttatc aacccctctt tggatcaact ccaacaatcc ctttttgatc
      361 ttttagtctg caccggtccc aaaggtgtca atatgattga agccgacggt aaaattgtca
      421 aaaatgacct tatgcttcag gctattgagt tggccaaaac caccggtgat gaggttaatt
      481 ctcagattgc ccagtttgcc gccaaagccg gcaaatccaa aattacattt gaacccgccg
      541 gaccatctgc cgaccttatc aaagacgttg agtccaaaat taaaactgat atcaaaaaat
      601 tctttgatga cggtgtcgat ggcgctcata tgatcggtca ggaaatgatt atagaaaaag
      661 tcaaacaact ttatcaggac aaaatcgatt ccgaagaagt ttctctaaat catcttgtgg
      721 ctgcagttga cgctattctc aaaaatcatg ttcgccagca aactctctct ggtcaccgtt
      781 atgaccggcg tcagttagac gaaatcagac ccttaaacgt tgaagctggg gttttaccca
      841 ccactcacgg ttctgctttg tttgaacggg gactaaccca ggctattact gtcactactc
      901 tggctcctct ctcagaaaaa caatatttag aagattccaa tggtgaagtt accaaacgtt
      961 atattcacta ttactctgct atgccttttt ccaccggtca aaccggacgt gtcggccgac
     1021 ccggtcgtcg ggaaattggt cacggagctc tagccgaaaa agccttagtt ccggttattc
     1081 ccaccgaagc tgaatttccc tatactatcg ttttgacttc cgaagttttg tctcaaaacg
     1141 gctcttcgtc tatggcttct acctgcggtt cgaccatgtc tcttatggac gccggcgttc
     1201 ctatcaaaga taaggttgcc ggtatttcta tcggtcttat ggccgaaact gatgacaaat
     1261 atattttgct aaccgatatt gctggtattg aagatcattt tggtgatatg gatttcaaaa
     1321 ttactggcac tcgcgacggt attaccgcta ttcaacttga tatcaaacgc gaaggtctga
     1381 ctctgcaaat gatccgcgat acttttgcgg cttccaccac tgcccgtctc cagattttgg
     1441 acaaaatgga ctcagttttg accgctccca gacccgagct gtctccgctg gctcccaaga
     1501 taaaattagt ccaattgccc gaagataaaa ttggtgaagt tatcggtagc ggcggtaaaa
     1561 ctatcaaagc tttaatggac aaatatcagg ttcaaattga cattgatgat tttggggccg
     1621 cttccatctc ttctcagaat caaaaatcaa ttgatgatgc cgcttacgaa attgaatcta
     1681 tgatcaaaga agttcaaatc ggcgaagaat atagtggtat tgttacccgg gtcgaaaatt
     1741 acggcgcttt tgttgaattc ttacccggac gtgaagcctt gcttcatgtt tccgaaatgt
     1801 ctggcggctt tttgtctgac ccttccacta ttatcaaagt cggcgacaaa ctcaatgtca
     1861 aaattgccgg tttcaatgac aatcatcaga tcaaattatc ttcacctgag tttaaggctg
     1921 cccaccccgg ccagcctcgg gaaaataaca atcgggctca gtttgctccc aaaccggaat
     1981 tccgcaaatt taaccccaat ttttccaaac gacctagata attgagtggt attttttagt
     2041 cgcattttag ttatactttt tatatggatt ctagtgtttt gactaccgct caggcgattg
     2101 aaactcttcg ccaaaaggtt aattcagtta aattgcccga taatttactt accaacctag
     2161 aagaactttt ttctcagctg gccatcaatt ctcgcaacga agaatcgttc tggcaaaatt
     2221 atcagtcagc ttcaaagtac attaattatc ttattagcgt tccttggttt agtcagactc
     2281 aagatattct tgacacccaa tttgcccgcg aaaaactcaa cgagcaacac tatggtttgg
     2341 ataccgtcaa agaccgtctt ttggaatata tctccgttct agctctccaa aaagaccgca
     2401 accccgataa tcccatcaaa gccccggttt tgttgtttgt cggcttggtc ggcaccggca
     2461 aaaccactat ggccaaatcc attgccagcg ttttgggtcg gcagttaatc cggattcctt
     2521 tcggcggttt gggtgacccg ctttatcttc gcggtcagtc tcgaattcat cctgaagccg
     2581 aacccggtca ggttatcaaa ggtctgatta attccaaaag tattaatccg gtaattctcc
     2641 tggacgaaat tgaccgggta gccgacgatg ctctcaatac cattatgggt gttttggtag
     2701 agctgcttga tccggagcag aacagtcact ttgttgatca ttatattgat tatccggttg
     2761 atttatcccg ggctattttt tgcgccacct gcaacaatac ctcccgaatt gccactgccg
     2821 ttatggatcg gcttgaagtt attcaaatgc cctcttacac cgacgaagaa aaaatagtta
     2881 tcggccgtga ctatattttc cccaaagtta tcaaagaagc cggtctccgt cccgaagaaa
     2941 ttaaaattga tgcttccgtt tgggatcaga ttgtccgtcc tttgggtttt gatgctggtg
     3001 tccggactct tcaacgcaat atcgaaggtt tatgccgcaa agtcgccaaa atgatttttg
     3061 aaggcaaaac caaacaggtt gttgtcgacg agtccaatgt taaatattat ctgccgcaat
     3121 ggtagaaatt aaataaaaat tatgattaaa aataaaaaaa atataaaacc gagagtcaat
     3181 ttttctggcg ctcaaaatca ggttagagaa tttaagtcta accaaaattc tgttcgtatt
     3241 agttctttgg gaggttttgg tgatgtcact caaaacatgt ttgtttatga atttatcccc
     3301 aacggcgaca cttccaaaag ccaaataatt attgtcgact gcggtgtcgg tttccccgaa
     3361 gaagatattt ttggcgtcga catgcaaatc cctgatactc attatcttga agacaaaaaa
     3421 gaccgaattt taggtatctt catcacccat ggtcacgaag atcacattgg tgctgtcaaa
     3481 tttattctcc cgattttggg cgaaaaaatt cccatttttg cccccaaact ggcagccgcc
     3541 tttattgaat cccgtcttag tgaagtcaat cagcgggcaa ttatcaacgt ctatgaagac
     3601 agtattcctg tcaagcgtgg agtttttagt gtcgatcctg ttcgggtgac tcattctatt
     3661 ccagacactt ttcatttatc gattaccact cctattggta ttttttacca tggttccgat
     3721 ttcaaattcg atgttttccc tcttgatggc aaaagagccg accttagtca catcgcccgt
     3781 atcggtgaaa aaaatgtcgt cgctcttatg tccgatagtc ttggttccga ccacgaaggt
     3841 tattctcctt ccgaaagttc aattgccgaa aattttaaat acgaaattac ctccgccaaa
     3901 ggtcgggttt ttgttacttc catctccagc aatatcatcc gctggtctca ggcgattgaa
     3961 tatgccaaaa gcgtcggccg gaaaatcgtt attgtcggtt tttctgttga caaggccatc
     4021 ggtattgcca aagacctcag ctacctcagt ttaaacgatt ctgatattgt tcctcccgaa
     4081 agagccaaaa actttcccga caataaactt atttttctgg tagcgggctt tgccggtcag
     4141 cctgattctg ctcttagcaa aatggttatg ggtaaacatc ggatcaaaat caaatccggc
     4201 gacaaggtta tcttttcctc tcccgactac attcccggca ctacttcaaa tatctacacc
     4261 atgattgata atctctctaa acttggggct gaagtcgtct atggtgaaaa agaagaactc
     4321 catgtctccg gccacggcta tcaaaaagag catgctcttc tcattagtct ggtcaagccc
     4381 aaatatctcc ttcccatcgg cggtaatttc cgccacgtca agcagtatca atccatggcc
     4441 aaaaagttgg gttatgctga agaaaatgtt attttgcctg actttgatgc ttcagttact
     4501 ttttatgaca atggcaagtt tgataccaat ttccatatcc ccatcaggaa aattttaatt
     4561 gacggttttg gtattggtga tgttggcact actgttctcc gcgaccgcaa gcttttggca
     4621 ggcgatggca tgttttctgt tgtgattctc atcaacaaag ccagtggtca aatggaaaaa
     4681 gatccggtag ttttgagccg gggatttgtt tttgtcaaag aaaataccga cttaatcaac
     4741 tatctcaagc aggaaaccat caaaaaattc aatgaagcca ccagcagccc tgccaatctt
     4801 gattatattc gagaagaaat tcaggctcat atcgaggaaa ttattcacca aaaaaccggc
     4861 cgccaaccca tggtcctgcc gttggtgatc gaggtctaaa ttaaagattt ttaattaatt
     4921 tctttttcat tttttctaaa tctttttctg atggcttata cctttcatca catctgattt
     4981 ctttttcaat cgcccaaata gcagtttctg gttttgggtc atttctgtag cgggggatta
     5041 aatgccagtg taaatgtgaa tcagtgttcc ctaacaattc ataatttaat ttttttggtt
     5101 taaaagtttt tatgaccgct tcagctactt ttgccatttc ttccaaaaat agtttttgtg
     5161 ttttacctaa tttatacaat tcatctatat gaattttgga gataaaaatt gtatagccat
     5221 aaaaatattg gtaatctgcc agcaccacat atccgctttt caactctctg acaaaatatt
     5281 gatttttgtt ttgcttaatc aattcaattc tttgacaaac aaaacagttg tttttattaa
     5341 tcattattta tattttatct ctttgttacg ttataataac tccatgagat attcacaact
     5401 ttttggtaaa accagcaaat ctgcccccgc cgatgccgac agtatcaacg ctaaattact
     5461 cactcaaggc ggctttatcc aaaaggaaat ttccggtgtt tacaattatc ttccccttgg
     5521 tctaagagtt ttaaccaaaa ttcaaaacat tattcgcgaa gaaatgaatc aaatcggcgg
     5581 tcaggaaatt ttgatgccgg ctttaaccac tgaagatagt tgggtgacta ccggccgtag
     5641 tcacatggat gttctgtttc atctaaaagg gcagggaaat accaacttgg ttctcaatcc
     5701 cactcacgaa gaagttgtca ccccattggc tcaaaaatat attttttcct accgcgatct
     5761 gcctttttcc gtctatcaaa ttcaaaataa atttagaaac gaacctcggg caaaatctgg
     5821 tcttttgcgg ggcagggagt ttaatatgaa agacctatat tcttttcatg ttgaccaaac
     5881 cgatttggac aaatattacg aagttgttaa gcaagcctat tttaaaattt ataaccggat
     5941 tggtttggga gatattactg ttctgacgtt tgcttctggt ggcactttta gtcagttttc
     6001 ccacgaattc caaactataa ataccaatgg cgaagatact atttatcttt gtgaaaaatg
     6061 ccaagttgcc gtcaacaaag aaattattga tgttcaaaaa acttgtcctg aatgtggcag
     6121 cgccgacctt aaaaccgtca aatccaccga agtcggcaat atttttaagc tcaaaaccaa
     6181 atattctcaa gctttcaatt ttgtttattc agatcaaaat gggcaggaaa aaattgttga
     6241 aatgggttgt tatggtcttg gtccgtcccg aaccatgggg acagtggtcg aagttttcca
     6301 cgatgacaaa ggtattattt ggccccagtc tattgcccca tatcaaattc acttgatcgc
     6361 tctagattta tttgaagata acctcaaaca agaagctgac aatctctatc aacatctact
     6421 ggacaagaat attgaagttc tttatgatga ccgtcccgat gtcgccgctg gcgccaaatt
     6481 tgccgacgct gatctaatcg gcattcctac ccgcttggtt gtaagcaaaa gaagtttagg
     6541 ggccggtgga gtcgaatcca aagacagaaa ccaatctcaa actaaaatta ttgccatcga
     6601 ttcactttag tatttagcag tctccaaaaa atactatata cttatttatg gctaaacgcg
     6661 gtcgcaaaaa aaagaaaatt attcccagtc tgcgattaaa accggaaact atccaaacta
     6721 tattttttat tttctttctt attctgacta tattgactgt tttttccttt ctccagactg
     6781 gacctattcc tgcccggatt aaccaaataa tcgtcaaata ctttggtctt ggttccgttt
     6841 taatcccatt tatccttttt ttggccgctt tcctttttgc caaaatcaaa acccctatca
     6901 aagatctcaa cgtctttttg ggttttttga ttatgtttat ctctcttatc agtctctttc
     6961 aacacggcat tgtcggcgcc tttctttttg accagctcgt atctttgttt ggtcgggttc
     7021 ccagtatcct tatttttatt ttttgtctta ttgttggttt tgtcat
//
LOCUS       DAOSHV010000023         6995 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_9384_length_6995_cov_5.742651, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000023 DAOSHV010000000
VERSION     DAOSHV010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 6995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6995
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9384_length_6995_cov_5.742651"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            77..1204
                     /locus_tag="PKZ29_01430"
     CDS             77..1204
                     /locus_tag="PKZ29_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37566.1"
                     /translation="MKKLKYIVFLLVAVVCLGLVQTVRAEPNVNDLFVAEENVNTTQD
                     LDGTAFRAGRNVKINSKVSGILFAAGENVSFEGESDYLFAAGRIVTVEGKVVNDAFVA
                     GSSVSLEEVSVGRDIYVGGENVSLSGDFERNVYAGGSVVLLGGNYKGDVTIEAQKIEI
                     VDGTKIGGKLKYNDTAELKIADGVVIGATETFKGVAPEENDVKEDLMKAKIKSIVFGL
                     VNGLVVGWLMIWLMPYVFEKLVEKNKQFSFSLAAKRFGWGLLWLIVLPILFVVSMVTM
                     VGIGAGMLMIFIYGLLIGLSGILVGYLVGWKLNLVEQNKTKVSKKYVDFAIGYLILTL
                     VRNIPVVGWLVSFLTVAIGLGLGFNLIIDRKKKAAVPAKSK"
     gene            complement(1206..2588)
                     /locus_tag="PKZ29_01435"
     CDS             complement(1206..2588)
                     /locus_tag="PKZ29_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37567.1"
                     /translation="MKKILFLILFLFFGLIFNKSILASSNIFGLHLTQTQDIHSAKDV
                     INSNGGDWGWVILVIRTDQLDYRTWQDFFDNCRKYHIIPIIRLATYSDQGNWKIPNPS
                     DIDNLANFLNSLNWPIKTQYVSLFNEVNHGQEWGGQVDVNSYVDLSIYASTKFKSLNS
                     NFFILNGALDLAAPNSMPKFLSAKSFYEQIYSYKPEFFDSIDGLASHSYPNHGFVGKP
                     YHTGQHSILGYQWELETIKKMGVTKDFPVFITETGWPHREGINQKNNFFTTKTTSQFL
                     LDAYQVWSTDNRIQAVTPFIFNYPHPPFDHFSWLDQDEQLYPEYQKVIDAAKSTNSPA
                     QITQYQLYRNQIPFLIFANHEYSGQISLKNTGQSIWGETNFCLQPTVSESVDVTQICT
                     NPSDKIHPGQIKIFPFKFKIKNNISQNISLSWEGLPETEINLFSENSTIYHPKINLWS
                     KLKNLFSKKD"
     gene            2608..3708
                     /locus_tag="PKZ29_01440"
     CDS             2608..3708
                     /locus_tag="PKZ29_01440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HOG37568.1"
                     /translation="MTKEEIIKMQISRIEKEIRDTPYHKATEHHHGILRAKLAKLKDD
                     LDGPVNRGGGGGVGYAIKHSGDASVVLVGLPSVGKSTLLNKVTNAESKVGNYDFTTLG
                     VVPGMMEYKGVKIQILDLPGIIEGAAGNKGFGRKVLSVIRASDLVVLMTDVDRIGWLE
                     KVEKELYNSGIRLNTKPPKIIVHKTHKGALQIVDPYYSFDKEIIIDVAKEMGLSNAII
                     QFGHKIENVDRLIDGLSKTRKYMAAVRIITKIDTVLDKKRLDKMEKDDVILMSADKNV
                     GIDEFKEKVWLGLGLVRVFLKKERNMEADKNNPLIMKNNSSLDDVLKRISSQMRDDVN
                     RAYIWGKSAKFPGQEVSFKFLVFDEMEVYFGR"
     gene            complement(3693..4748)
                     /locus_tag="PKZ29_01445"
     CDS             complement(3693..4748)
                     /locus_tag="PKZ29_01445"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HOG37569.1"
                     /translation="MSPVQKTFLSFSFGCRVNAAETNQWSQILLNQGFLPATNQDKPS
                     IILINTCSITKKGEKESLNKVKFLSQKYPKAKIYVTGCADFTKIKNLKNISFFTNPDK
                     EKLLKNLNSLYSPKIKDKFSRTKRFLLKIQSGCTQYCSYCIVPFKRNYLYSLPINQAI
                     NTVKQAIKDDYREVIITGINIDQYQYKFTSLLKDILDKTSIKLISFGSVPINCIDNHL
                     IELYQIYPDRLSKFIHIPIQSGSDKILKLMNRPYTKKDILTKIHYLKSVIKDINLGTD
                     IIVGFPGETKSDFNQTLQLCHQIGFSKIHTFIFSPRPNTKARILFENSPKIKKEILKN
                     RSLQIRLAQNQKNYRPK"
     gene            complement(4773..5648)
                     /locus_tag="PKZ29_01450"
     CDS             complement(4773..5648)
                     /locus_tag="PKZ29_01450"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOG37570.1"
                     /translation="MTKTILVTGGAGHIGSHVIEKLLNYSQYKIISLDNYFTGSPDNH
                     LKGADYRRGHTKDIDKLIPETPEIVFHLGEYARVAPSLDEPEKVYDMNVLGTFAVVEF
                     CRRRKVGKLVYAASSTKFASNGQERHANPYSFTKANNVDLINNYGKWYQLPYAICYFY
                     NAFGPRENGVGKYATLIAKFEQAYLKHQKLPIIKPGTQKRNFTYVGDLAAGMILAAQK
                     GSGDGYALNNPKAYSVIEIAQAFDSPVEYINGYPGRTESGDSPTKANQLGWKTTIDVI
                     DYIQTFIKDHPKIEK"
     gene            complement(5651..6748)
                     /gene="tgt"
                     /locus_tag="PKZ29_01455"
     CDS             complement(5651..6748)
                     /gene="tgt"
                     /locus_tag="PKZ29_01455"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOG37571.1"
                     /translation="MLLQPTFVPVGTQATVKSLSPEELTQIGVEIFFCNTYHLYLRPG
                     DKFIKKLGGLHQFTRWQGQLITDSGGFQVFSLAKEHNGAGNLVKITEDGVEFRSHLDG
                     SLHFFTPEKSLQIQHNLAADYIIAFDECTPYPATKKYAKKALDRTHRWAIRSLAEHKK
                     LNQKLNQNLKLYGVIQGSYYQDLRQESANFICSQDFDGIAIGGVSVGEPKDKMRQAVS
                     YALPTALKSGKPIHLLGVGEIDDLFDFFGQGVTSMDCTLPTRMARGGQFLHRTGNSTY
                     DQRNKFKSQIMNSKYKLDQTPLDPDCHCPACQKYDKAYLHHLFKTQELLGYRLMTLHN
                     LSFMINTTTQIRNSLKDGTFSKLKYNWLGRN"
     gene            6815..6899
                     /locus_tag="PKZ29_01460"
     tRNA            6815..6899
                     /locus_tag="PKZ29_01460"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6849..6851,aa:Leu,seq:taa)
BASE COUNT         2109 a          938 c         1774 g         2174 t
ORIGIN      
        1 acggcttagg accgtgtggg gtaacccatg gaggttcgag tcctctctgc ggcacatttt
       61 gtgtataatg gggtatatga aaaagttaaa atatatagtg tttttgttag tggcggtagt
      121 ttgtttaggt ttagttcaga cagtaagagc agaaccaaat gtaaatgatt tgtttgtggc
      181 tgaggaaaat gttaatacga cacaagatct ggatgggaca gcttttcggg cgggaaggaa
      241 tgtaaaaatt aattctaaag taagtggaat attgtttgca gccggagaaa atgtgtcttt
      301 tgagggagaa agtgattatt tgtttgctgc cggtagaata gttacggtag aaggaaaggt
      361 ggtaaatgac gcttttgtag caggatcaag tgtgagttta gaggaggtat cggtaggtag
      421 agatatatat gtaggtgggg aaaacgtatc tttgtcgggt gattttgaaa gaaatgtata
      481 tgcgggagga tcggtggtat tgctgggtgg aaattacaag ggggatgtaa caattgaagc
      541 ccaaaaaatt gaaattgttg atggaactaa aattggggga aaattgaaat ataatgatac
      601 ggcagaatta aaaatagctg atggagttgt aataggggca acagagactt ttaaaggggt
      661 ggcgccagaa gaaaatgatg ttaaggagga cttaatgaaa gctaaaatta agtcaattgt
      721 gtttggtttg gttaacggac tagtggtggg ttggctgatg atttggttga tgccttatgt
      781 ttttgaaaaa ttagtggaaa agaataagca atttagtttt agtttggcgg ctaagagatt
      841 tggctggggt ttgctatggt tgatagtttt gccgattttg tttgtagttt ctatggttac
      901 aatggtggga attggagccg ggatgttgat gatatttata tatggtttac tgatcggttt
      961 gtcaggaata ctagtgggtt atttggttgg ttggaagttg aatttggtgg agcaaaacaa
     1021 aactaaggtc agtaaaaaat atgttgattt tgccattggg tatttgattt tgactttggt
     1081 aagaaatatt cctgtagttg gttggttagt gtcgtttttg acagtggcta taggtttggg
     1141 attaggattt aacctaataa ttgatcgcaa gaaaaaagct gcagtaccgg caaagtcaaa
     1201 ataatttaat cttttttaga aaataggttt ttgagcttag accagagatt aattttgggg
     1261 tggtagatgg ttgagttttc ggaaaaaaga ttaatttcgg tttcaggtaa accctcccag
     1321 gacagagaaa tattttgaga gatattgttt tttattttga atttaaaggg aaagattttg
     1381 atctgtccgg ggtggatttt gtctgaggga ttggtgcaga tttgggtaac atcaacagat
     1441 tcggagacgg ttggttgaag gcaaaaatta gtttcacccc aaattgattg gccggtattt
     1501 tttaggctga tttgacccga atattcgtgg ttggcgaata taaggaaggg aatttgattc
     1561 cgatagagtt ggtattgagt gatctgtgcg ggggaattgg ttgatttggc ggcatcaata
     1621 actttttgat attcaggata aagctgttca tcttggtcca gccaagagaa gtgatcaaaa
     1681 ggtgggtggg ggtagttgaa gataaatgga gtgacggctt gaatccgatt gtcagttgac
     1741 caaacttggt aggcatcaag cagaaattga gaagtggttt tggtagtaaa gaagttattt
     1801 ttttgattga tcccttcgcg atgaggccaa ccggtctcgg taataaagac aggaaaatct
     1861 ttagtaaccc ccattttttt gatagtttca agctcccact gatagccaag aatactgtgt
     1921 tgaccggtat gataaggttt gcccacaaaa ccatgattgg ggtaagaatg ggaagctagt
     1981 ccgtctatag agtcaaaaaa ttcgggcttg tagctataaa tttgttcata aaatgatttg
     2041 gcggagagaa atttaggcat agaattgggg gcggccaggt caagagcgcc attgagaata
     2101 aaaaaattag aattaagaga tttgaatttg gttgaagcat aaatactaag atcaacatag
     2161 ctattaacat cgacctggcc gccccattcc tgtccatggt taacttcatt aaacaaagag
     2221 acatattgag ttttaatagg ccaattaaga gagttgagaa aattagctaa attatcgata
     2281 tctgatgggt tggggatttt ccaattaccc tgatcggaat aagtagccag gcgaataatg
     2341 ggaataatat ggtattttcg gcaattatca aaaaaatctt gccaggtgcg ataatcgagt
     2401 tgatcagtgc ggataactag aataacccag ccccaatcac cgccattgct attgatgaca
     2461 tctttggcgg aatggatatc ttgtgtttgg gtgagatgaa gaccaaagat gtttgaggat
     2521 gctaaaatag acttattaaa aataagccca aaaaatagaa ataaaattaa aaataagatt
     2581 tttttcaaag taagttaatt ttagcaaatg accaaagaag aaattattaa aatgcagata
     2641 tctcggattg agaaggaaat tcgtgataca ccttatcata aggccacaga acatcaccac
     2701 ggtattctgc gggcaaagtt ggccaaactc aaggatgatt tggacggacc ggtaaatagg
     2761 ggtggcggtg gcggtgtagg ctatgcaatc aagcattctg gggatgcttc agtggtatta
     2821 gtgggattgc caagtgtggg caaatcgacc ctgcttaata aagtaacaaa tgccgagtcg
     2881 aaagtgggaa attatgattt tacaaccttg ggggtggtgc cggggatgat ggaatataag
     2941 ggggtaaaga ttcagatttt ggatttaccg ggaattattg aaggggcggc gggtaataaa
     3001 ggctttggaa gaaaggtgtt gtcggtaatc agggcctcag atttggtggt gttgatgaca
     3061 gatgttgata gaattggttg gttggaaaag gtggaaaagg agttatataa ctcaggtata
     3121 aggctaaata ctaagccgcc aaagataata gttcacaaga cccacaaggg agcgttgcag
     3181 atagttgacc cttattattc ttttgataaa gagatcataa ttgatgtggc taaggagatg
     3241 ggattgtcga atgccataat ccagtttggg cataaaattg agaatgtaga taggttgatt
     3301 gatggtttaa gtaagacgcg aaaatatatg gcggcggtaa ggataattac aaaaattgat
     3361 actgttttgg ataaaaaacg gttggataag atggaaaaag atgatgtaat tttgatgagt
     3421 gcagacaaaa atgtgggaat tgatgagttt aaggaaaaag tttggttggg attggggtta
     3481 gtgcgggtat ttttaaaaaa agagagaaat atggaggcgg ataaaaataa tccattgata
     3541 atgaaaaaca attcaagttt agatgatgtt ctaaaacgga tatctagtca aatgagagat
     3601 gatgttaatc gggcttatat ttggggcaaa agcgctaaat ttcccggtca ggaagtgtcg
     3661 tttaaatttt tggtgtttga tgagatggaa gtttattttg gacgataatt tttttgattt
     3721 tgggctaatc tgatttgcag ggatcggttt tttaggattt cttttttaat tttgggcgaa
     3781 ttttcaaata aaattctggc tttggtgttg ggacggggtg aaaaaataaa ggtatgaatt
     3841 ttggaaaatc caatttggtg gcagagttgt agagtttgat tgaaatcaga tttagtttcg
     3901 ccagggaagc cgacaataat gtcggtgcct aaatttatat ctttgataac agattttaaa
     3961 taatggattt tagttaaaat atcctttttg gtataagggc ggttcattaa ttttaaaatt
     4021 ttgtctgatc ccgattgaat tggtatatgg ataaatttgg ataatctgtc gggatatatt
     4081 tggtagagtt caattagatg gttatcgata caattaattg ggactgaccc aaaactgatt
     4141 agttttatgg atgttttatc taaaatatcc tttagtaagg atgtaaattt atattgatat
     4201 tggtcaatat ttattccagt aatgatcact tccctatagt catctttgat agcctgtttg
     4261 acggtattga tggcttggtt aatgggtaaa gaataaagat aatttcgttt gaaaggaaca
     4321 atgcaataag aacaatattg agtgcagcct gactgaatct tgagaagaaa tcttttggtg
     4381 cggctgaatt tatctttaat tttgggacta taaagagagt tgagattttt aagaagcttt
     4441 tctttgtcgg ggttggtgaa aaaagaaata ttttttaggt tttttatttt ggtaaagtca
     4501 gcacaaccag taacataaat ttttgctttg ggatattttt gggataaaaa tttaaccttg
     4561 ttgagcgact ctttttctcc ttttttggtg atagaacagg tattaattaa aataatagat
     4621 ggtttgtcct ggtttgtggc cggcaaaaaa ccttgattta ataaaatttg ggaccattgg
     4681 ttggtctcgg cagcgttaac acgacaacca aaactgaaag aaagaaaagt tttttggaca
     4741 ggagacatgg tcaatattat agcaaattaa gcctattttt cgattttggg gtgatctttg
     4801 ataaaggttt gaatatagtc gataacatca atggtggttt tccaccctaa ttgattggct
     4861 ttggtaggac tatcgcccga ttcggtgcgg ccgggatagc cattaatata ttcaaccgga
     4921 ctgtcaaagg cttgggcaat ttcaattaca gagtaagctt tgggattgtt gagggcatag
     4981 ccgtctccgc tgcctttttg ggcggctaaa atcatgccgg cggctaagtc gccaacatag
     5041 gtaaaatttc ttttttgggt ccctggtttg ataatgggaa gtttttggtg tttaagataa
     5101 gcttgttcaa atttggcaat taaagtggca tacttcccta ccccattttc tcttggtcca
     5161 aaggcattat aaaaataaca aatagcataa ggcaattgat accatttgcc gtaattattg
     5221 attaaatcaa cattattagc tttggtaaaa gaataaggat tagcatgacg ttcctgaccg
     5281 tttgaggcaa atttagtgct ggaagcagca taaaccagtt tgccaacttt tcggcggcgg
     5341 caaaattcaa ccacagcaaa ggtgcctaaa acattcatat cataaacctt ttccggttcg
     5401 tcaagagaag gggcgactcg ggcatattca cccaaatgaa agacaatttc tggagtttcc
     5461 gggattaatt tgtctatatc cttggtatga cctcggcggt agtcggcacc tttaaggtga
     5521 ttatcaggac tgccggtaaa ataattgtct aaagatatga ttttgtattg ggaataattg
     5581 agtagttttt cgataacatg ggagccaatg tgtccggcgc cgccggtaac caaaattgtt
     5641 tttgtcataa ttaatttcga cctaaccaat tatacttgag tttgctgaag gtgccatctt
     5701 ttaaactatt tctgatttgg gtggtagtgt taatcataaa agagaggttg tgtagggtca
     5761 tcaggcggta gccgagcaat tcttgggttt taaagagatg gtggagataa gctttgtcgt
     5821 atttttgaca ggcaggacag tgacagtcgg ggtcgagagg agtttgatct agtttatatt
     5881 tgctgttcat aatttggctt ttgaacttat ttctttgatc ataggtagaa ttgccggtgc
     5941 ggtgaaggaa ttgtccgcct cgggccatac gggtaggaag ggtgcagtcc atggaggtaa
     6001 ctccctgacc aaaaaaatcg aataaatcat caatctcacc aactcctaac aaatggattg
     6061 gcttgccact tttgagagca gtcgggagag cataagaaac agcttgacgc attttgtcct
     6121 tgggttcacc aaccgagaca ccgccgatgg caatgccgtc aaaatcttga gaacaaataa
     6181 agttagcgga ttcctgcctt aggtcttgat aataacttcc ttgaataacg ccgtatagtt
     6241 tgagattttg gttgagtttt tggtttaatt ttttgtgttc agcaagcgat cggatggccc
     6301 agcggtgggt tcggtcgaga gcttttttgg catatttttt ggtagcagga tagggggtgc
     6361 attcgtcaaa ggcaataata taatcagcgg ctaaattgtg ttgaatttga agggattttt
     6421 ccggagtaaa aaaatgcaaa gaaccgtcaa gatgagatct gaattcaacc ccatcttcgg
     6481 taattttgac tagattgcca gcgccattat gctctttggc tagggaaaaa acctgaaagc
     6541 caccggagtc agtaataagc tgaccttgcc atcgggtaaa ctgatggaga ccaccaagtt
     6601 tttttataaa tttatcgcca ggtctaagat aaagatggta ggtgttgcag aaaaaaattt
     6661 caactccgat ttgggttagt tcttcgggag aaagcgactt gacagtggct tgggtgccga
     6721 cagggacaaa agttggctgt aagagcatag attagattat aacattggct taaactgtaa
     6781 aaaaaatcgg catcttgtta taataatgaa cgttgccgca gtggtgaaat tggtatacac
     6841 gcaagactta aaatcttgtg ctctcaacaa gcgtgtgggt tcgattcccg cctgcggcac
     6901 cgggtcagta tttggcataa aatacttaat actgagatct atcggggatt ggcgcaattg
     6961 gctagcgtgc atggtttggg accatgaggt tgcag
//
LOCUS       DAOSHV010000024         6741 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_9859_length_6741_cov_5.664822, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000024 DAOSHV010000000
VERSION     DAOSHV010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 6741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6741
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9859_length_6741_cov_5.664822"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            112..792
                     /locus_tag="PKZ29_01465"
     CDS             112..792
                     /locus_tag="PKZ29_01465"
                     /inference="COORDINATES: protein motif:HMM:NF024976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HOG37572.1"
                     /translation="MAKTITKQNILNLTKGLQFNTEDELRDYLVPKLAKLFKVKESQV
                     ERESITTSFDNTLSNRADIIIRTEGENFKKAFIVFELKLSKNVEIFKNGDYTDADKQL
                     KKYCQDVRAPYGVLLTENFCAIYRNKYYSYDQEPRRVEKEEIPTISQIENLMAREAIL
                     DVSFYNKSFKYILLVILSLFVLKIIIDMIKIVFNLNWVMTSIFSFLSGIMIALMLMVI
                     FRIRKTFD"
     gene            814..1434
                     /locus_tag="PKZ29_01470"
     CDS             814..1434
                     /locus_tag="PKZ29_01470"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOG37573.1"
                     /translation="MKKWWVILVVVILGVWGWWSQRESPVEREDEPLQEKIPETKVVV
                     EKEIVAPIDEFKERITKKKFGDYISPKNSPISPERFEGFHTGVDVEYGDSDQEIEVRS
                     IAVGKVVGSRKAQGYGGVVIVEHQIGDQILFGIYGHLKPESMARVGEEVKAGQKIGVL
                     GKDYSTETDNERKHLHLGLSKTNNIRGYVNNKAELDNWVDPLGLID"
     gene            1473..2192
                     /locus_tag="PKZ29_01475"
     CDS             1473..2192
                     /locus_tag="PKZ29_01475"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HOG37574.1"
                     /translation="MGKKKWLKFDLHMHSRHSDGVSSVREMILAAKTKGLDVIAITDH
                     NLAVKIETEKIYKKYGIYVIPGCELSFFRGHFLVLGLEAEIVNKKIKEMGIRETTTTM
                     MNRRKTIESLLQFFVKKGALVIAAHPKIPTGIMSARKSFLIKLYKKGLIHGAETHNGN
                     LERKFGRRVYWVWHKMAKKTMIKLNMPPYADSDAHVASRIGYRFNLVQMTDPRKLLEV
                     LKKGRIEIKHGTGGSLKSGDR"
     gene            complement(2194..2520)
                     /locus_tag="PKZ29_01480"
     CDS             complement(2194..2520)
                     /locus_tag="PKZ29_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37575.1"
                     /translation="MDSYNFFLLFGLTAGLYLISAFLVKFKKLSPVTQRRFWNLVLLI
                     SFLVSGLTGLFFAFAIDQNRHLPNYLTGLWLHVEFGIVMATIAIFHALWHLPYFRGIF
                     PTKPKK"
     gene            complement(2524..2859)
                     /locus_tag="PKZ29_01485"
     CDS             complement(2524..2859)
                     /locus_tag="PKZ29_01485"
                     /inference="COORDINATES: protein motif:HMM:NF024763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HOG37576.1"
                     /translation="MTQKKLALIFVVGLSIISGIIFVSLSKSRSQTTSSNSKSDQQTQ
                     ISPTPQTTAQLSVLPHQCIGCGRCRRLDPQHFDLQNRQAVVISSLNLDSPALKSAITS
                     CPTQAIVLK"
     gene            3043..3621
                     /locus_tag="PKZ29_01490"
     CDS             3043..3621
                     /locus_tag="PKZ29_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37577.1"
                     /translation="MPTFKKLHLQLKKIQKRISLPQISLPDLEGLTQETKKILDETVK
                     SFAYYKNLIPKVTVRRSFWRYLLSAPFIYGMIVPALIWNLGLEIYHQICFRLYGIPLV
                     KPAEYFVYDRRLMSFLNPWEKINCYYCSYVNNLIRYSAEIGGRTERFWCPIKYHRRLE
                     KSHSQYAKFIGRDDKKNFRKDWDKLRDFSDVK"
     gene            3653..4261
                     /locus_tag="PKZ29_01495"
     CDS             3653..4261
                     /locus_tag="PKZ29_01495"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOG37578.1"
                     /translation="MIKAIIFDFGNVICHFTNQILVEKIAKISRKCEEKIFDLIYRQT
                     DVVEKYESGLISSVEFYQKISKLCGINIPYFELKRIYSEDKFTPVKGMEELVKKLKRN
                     YKIGLLSNTSEWDWDYMVKSAPYINGFESITKSYEARAMKPKTKIFEDAVVKLGLKPE
                     ECVFTDDVKEYVAAAKSFGLRAYVFTTTDKLMKDLEKLGLKS"
     gene            4306..4797
                     /locus_tag="PKZ29_01500"
     CDS             4306..4797
                     /locus_tag="PKZ29_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37579.1"
                     /translation="MKKLIKKAEKMETNDLTKMLTDVENKLNEIFGKKAPKMPESVVD
                     FLVKYGPYLIIVGLILTVLTVFQTIGWTSRLAPMNRWGGYNYGFGPKFWLMEILMLVP
                     AAMRAMAVSPLMKRQIRGWRLMFWASLVSVVIGILRLELGGALLGAIISWYILFQIKK
                     EYK"
     gene            4800..5270
                     /locus_tag="PKZ29_01505"
     CDS             4800..5270
                     /locus_tag="PKZ29_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37580.1"
                     /translation="MIERKYNQETSELAEAGLSSTRFLAKEGEKIVSQALVVEYKNKT
                     DIPFGYVVYVQTQQEYRGLGLAGGVMDKVNNFLEGKEMVGVLENKISKNDPGFRLYQD
                     RGWRESKKYENWMIYDRKQTSEAQINEVIEYLINQKIEDEYDFEIKEIDIDRRS"
     gene            5302..6450
                     /locus_tag="PKZ29_01510"
     CDS             5302..6450
                     /locus_tag="PKZ29_01510"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011011995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOG37581.1"
                     /translation="METKGKLNLITRNCEEVLTGEDLEKLIESGTELRHYIGFEISGL
                     VHLGTGLMSMGKIADFLKAGVRCQILLADFHSYLNNKLGGDWDNIRTATEGYFKSGLI
                     ASLACFGVEGKEIEFITGKDLYRDNLTHWETFMEVGKHVTLSRNLRSISIMGKSQGKD
                     VDMATLFYPPLQVADIFSLKANIIHAGMDQRKAHVIAKDVAKKLKINPLKNNQGEVIS
                     PVAVHHKLIAGLTGPEPASRQGSDLSQNNSNESLKMSKSKPNSAIFIHDTPDEIRNKI
                     KKAYGPPKEIEFNPLINWVETLVFWGEKKGNFKISRPERYGGNVVYTEIDKLISDYKS
                     EKLYPADLKNGLAEWLIEKLEPARKYFERPEAKEGWIKMKEFLDIKSP"
     gene            6477..>6741
                     /locus_tag="PKZ29_01515"
     CDS             6477..>6741
                     /locus_tag="PKZ29_01515"
                     /inference="COORDINATES: protein motif:HMM:NF014707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HOG37582.1"
                     /translation="MKILVHSCCADCLLKLVEAMKQESDKNLELTAYVYNPNIHPRSE
                     YLSRLKAVQQIAKEIKIELVVADWSPKEWFEVNKNKFEKPERCH"
BASE COUNT         2317 a          864 c         1701 g         1859 t
ORIGIN      
        1 ccgccgagtc caaattagtt ttcagaattt tcgtcaaaaa ttgttcgaac ttcgttcaaa
       61 aaacaccgca aggattaaat tatttttgca gaaagcaatt gagctatgtt aatggctaaa
      121 actattacta aacaaaacat attaaattta acgaagggtt tgcagtttaa tactgaggat
      181 gagcttaggg attatttggt gccaaagctc gcaaagttat ttaaggtaaa agaaagtcag
      241 gttgaaagag aatcaataac taccagtttt gacaatactc tatcaaaccg agctgacata
      301 ataatcagaa cagaaggaga aaattttaaa aaagcattta tagtttttga gctaaaattg
      361 tcgaaaaatg tggaaatatt caaaaatgga gattacactg atgctgacaa acaattaaag
      421 aaatattgcc aagatgttag agctccttat ggagttttat taactgaaaa tttttgtgct
      481 atttatagaa acaaatatta ttcttatgat caggagccaa gaagggttga aaaagaagaa
      541 atacctacaa ttagtcaaat tgaaaacctt atggcaagag aggcaatttt agatgtcagt
      601 ttttataata agtcattcaa atacattctc ttagtaattt taagtctttt tgttctaaaa
      661 ataattattg acatgataaa aattgttttt aacttaaact gggtaatgac atctatattt
      721 tcctttttaa gtggaataat gattgcttta atgttgatgg ttatttttcg aatccggaaa
      781 acttttgatt agttttaaca ggtataattg agcatgaaaa agtggtgggt gatattagtg
      841 gtagtgattt tgggggtgtg ggggtggtgg agtcaaagag agagtccggt tgagagagaa
      901 gatgagccat tacaagaaaa aataccggaa acaaaggtgg tagtagaaaa agaaatagtg
      961 gcgccgatag atgagtttaa agagagaata actaagaaaa aatttggtga ttatatatcg
     1021 ccaaaaaatt cgccgatttc tcccgagaga tttgagggtt ttcatactgg ggtggatgtg
     1081 gaatatggcg acagcgatca ggaaatagaa gttagatcaa tagcggtagg caaggtggtg
     1141 gggtcaagaa aagcacaagg ttacggtggg gtggtgatag ttgagcacca aattggggac
     1201 caaatcttgt ttggaattta tggacacttg aaacctgaga gtatggcaag ggtgggagaa
     1261 gaagtgaaag cgggtcaaaa aataggagta ctgggaaaag attatagcac agagactgat
     1321 aatgaacgaa agcatttgca cttgggatta tcaaagacaa acaatatcag aggttatgtg
     1381 aataataagg cggaattgga caattgggtt gacccgttgg gattgattga ttagggttgg
     1441 tttgaaatta atttagtgat ataaacaacg ttatgggcaa aaaaaagtgg cttaaatttg
     1501 acctgcatat gcacagccga cacagcgatg gtgttagtag tgtaagagag atgattttgg
     1561 cagccaaaac taaggggttg gatgtgatag cgattactga tcacaatttg gcagttaaaa
     1621 ttgagacgga aaaaatatat aaaaaatatg ggatttatgt gattccgggg tgtgaattgt
     1681 cattttttcg ggggcatttt ttggtgctgg gactggaggc agaaatagtt aataaaaaaa
     1741 tcaaagagat gggaataagg gagacaacaa ccacgatgat gaaccggaga aaaacaatag
     1801 aaagtttatt acaatttttt gtaaaaaaag gagctttggt aattgcggcc catcccaaaa
     1861 taccaaccgg gattatgtca gctagaaaaa gttttttgat aaaactgtat aaaaaaggat
     1921 tgattcatgg agcggagact cataatggca atttggagag aaagtttggc aggagagtct
     1981 attgggtgtg gcacaaaatg gccaaaaaga cgatgataaa gttaaatatg ccgccatatg
     2041 ctgacagcga tgctcatgta gccagcagga ttggatatcg atttaatctg gtgcagatga
     2101 cagacccaag aaaattgctg gaagtgctaa agaaaggtag aatcgagatt aaacatggga
     2161 cgggcgggag tttgaagtcg ggagacagat aaattatttt ttgggtttgg ttgggaaaat
     2221 accgcgaaaa tagggcaagt gccaaagggc gtgaaagatg gcgatggtgg ccataacaat
     2281 accaaactca acatgaagcc aaagacccgt aaggtagttg gggaggtggc gattttgatc
     2341 aatagcaaag gcaaaaaaga ggccagtaag accggaaact aaaaaagaaa ttaaaagaac
     2401 caggttccaa aaccggcgtt gagtgacggg agaaagtttt ttgaatttga ccaaaaaagc
     2461 ggaaattaaa taaaggccgg cggtgagacc gaaaaggaga aaaaaattgt agctgtccat
     2521 aatttatttt aggacaatgg cttgggtagg gcaagaagta atggctgatt ttagagccgg
     2581 agagtcgaga ttaagggaag agataaccac agcctggcga ttttggaggt caaaatgctg
     2641 tgggtcaagt cggcgacaac gaccacagcc gatacattgg tgaggcagaa ctgaaagctg
     2701 agcggtagtt tggggagtgg gagaaatttg ggtttgctgg tcagatttgg aattggagga
     2761 ggtggtttga gagcgggatt ttgaaagaga aacaaaaata atcccggaaa taattgataa
     2821 accaacaaca aaaatgagag ctaatttttt ttgggtcata agagattttg ttttatgtta
     2881 taaaattgac taagatgcaa tatgtttttg atctgataaa tatttttatc actttttttg
     2941 taagtttatg gaactggttt gtgtctttgc cgcttttagg taaggttata gtggtaattt
     3001 taattttgtc cggagctttt agctctgcca gtcgctaatt ttatgccgac tttcaaaaaa
     3061 cttcatttac agctaaaaaa aatccaaaag cgtatttccc tacctcaaat tagtctgcca
     3121 gatttggaag gtctaactca ggaaactaaa aagattttgg atgagacagt caagagcttt
     3181 gcctattaca aaaacttgat tcccaaagtt actgtaagac ggtcgttttg gcggtatttg
     3241 ttgtcggcgc cttttattta cgggatgata gtgccggctt tgatttggaa tttgggactg
     3301 gagatttatc atcagatttg ctttaggctt tatggtattc ctttggttaa accagcggaa
     3361 tattttgttt atgatcggcg gttgatgtct tttctaaacc cgtgggagaa aattaactgt
     3421 tattactgtt cgtatgtgaa taacttgatt cgttacagcg cggaaattgg cggacggaca
     3481 gaacgttttt ggtgtccgat aaaatatcac cgccggctgg aaaaaagtca ctctcaatat
     3541 gccaaattta ttggtcggga tgataaaaaa aactttcgga aagattggga taaacttcgc
     3601 gacttttcgg atgtaaaata aagtttttgg ttagttttta aaaatttatt ttatgatcaa
     3661 ggcaataatt tttgattttg gcaacgtaat ttgtcacttt accaatcaga ttttggtgga
     3721 aaaaattgct aaaatatcga gaaaatgtga ggagaagata tttgatctga tttatagaca
     3781 aacggatgtg gtggaaaaat acgaaagcgg attgataagt tcggtggaat tttatcagaa
     3841 aatttcaaaa ctatgcggca taaatatccc ctattttgag ttaaaaagaa tttattcgga
     3901 ggataaattt actccggtta agggcatgga agagttggtt aaaaaattaa aacggaatta
     3961 taaaatcgga ctgctgtcca atacaagcga gtgggattgg gattatatgg tgaagtcggc
     4021 gccgtatata aacggttttg aatcgataac taaatcatat gaggcgagag cgatgaaacc
     4081 aaaaacaaaa atatttgagg atgcggtggt gaaactggga cttaaaccgg aagagtgtgt
     4141 gtttacggat gacgttaaag aatatgtggc ggcggcaaaa agctttggct tgagagcata
     4201 tgtgtttacc acaacagata aattgatgaa ggatttggag aaattggggt tgaaaagtta
     4261 aaagggtgat tgatttttgg ggcaaggata gtaaaattgg ttaatatgaa aaaattgatc
     4321 aaaaaagcag aaaaaatgga gacaaatgat ttgacaaaaa tgttgactga tgtggaaaat
     4381 aagttgaatg aaatttttgg caagaaagcg ccaaaaatgc cggaatcggt ggttgatttt
     4441 ttggttaaat acggacctta tttgattata gtgggattaa tattgacggt gttgacggtt
     4501 tttcagacta tcggctggac atcaaggttg gcgccgatga acagatgggg tggttacaac
     4561 tatggttttg gtcctaagtt ttggctgatg gaaatattga tgttggtgcc agcggcaatg
     4621 cgggcaatgg cggtgtcacc attaatgaaa agacaaatta gggggtggag attgatgttt
     4681 tgggcttcgt tggtgagtgt ggtgataggg atcttgagac tggaattagg cggggctttg
     4741 ttgggagcca taatttcttg gtatattctg tttcagatca aaaaagaata taaataaagg
     4801 tgattgaaag aaaatacaat caagagacgt cagaattggc tgaagcgggt ttgagttcga
     4861 ctagattttt ggcaaaagag ggagaaaaaa tagtctcaca ggcgttggtg gtggaatata
     4921 aaaacaagac agatatcccg tttggatatg tggtctatgt tcagactcag caggaatata
     4981 gaggtttggg gttggctggc ggggtgatgg acaaggtaaa taattttttg gagggaaagg
     5041 agatggtagg agtgttggag aataaaatta gtaaaaatga ccctggtttt aggctgtatc
     5101 aggaccgcgg ctggcgggag tcaaaaaaat atgaaaactg gatgatttat gacagaaaac
     5161 aaacatcaga agctcaaata aatgaagtga ttgaatattt gattaaccaa aaaattgagg
     5221 acgaatatga ttttgaaata aaggagattg atattgacag acggtcatag aaaaaaaccg
     5281 ggtttcagct tataatcggt tgtggaaaca aaaggaaaac taaacttaat tacccgaaat
     5341 tgtgaagagg tgctgacggg agaggatttg gaaaaattaa ttgagagcgg gacggaattg
     5401 agacattata tcggctttga aatttccgga ttggttcatt tgggcacagg attgatgtcg
     5461 atgggaaaaa tagctgattt tttgaaagcg ggagtgagat gtcagatatt gctggcagac
     5521 ttccattcat acttaaacaa caaattgggc ggagactggg ataatatccg aaccgccact
     5581 gaaggctact ttaaaagcgg gctgatagcg tcgttggcgt gttttggggt ggaaggaaag
     5641 gaaatagagt ttatcaccgg caaagattta taccgggata atttaaccca ttgggaaacg
     5701 tttatggagg tagggaaaca tgtgactttg tcaagaaatt tgagatcgat ttcgattatg
     5761 ggtaaaagtc aaggcaagga tgtagatatg gcaactctgt tttatccccc gctgcaagtg
     5821 gcagatattt ttagtttgaa ggccaacatt attcatgcgg ggatggatca gcgcaaggcc
     5881 catgtaattg ctaaagatgt agccaaaaaa ctaaaaatta atccgctgaa aaataaccag
     5941 ggagaagtga tatcgccggt ggcagtccac cataaattga ttgcgggatt gaccggacca
     6001 gaaccagcct cgcgacaagg ttctgattta tcccagaata atagtaatga gtcactgaaa
     6061 atgagcaaat caaaaccaaa tagtgctatt tttattcatg acacgccaga tgaaatcaga
     6121 aacaaaatta aaaaggccta tggcccgcca aaagaaattg aatttaaccc gctgataaac
     6181 tgggtagaga cgctggtgtt ttggggagaa aaaaagggta attttaaaat ttccaggccg
     6241 gaaagatatg gtggaaatgt tgtttataca gaaatagata aattaataag cgactataaa
     6301 tcggaaaaat tgtatccggc ggatttgaaa aacgggttgg cagaatggtt gatagaaaaa
     6361 ctggagccgg ctagaaaata ttttgaaagg ccggaggcaa aagaagggtg gataaaaatg
     6421 aaagagtttt tagatattaa atctccttaa acacttttga caagagaggt tggtttatga
     6481 aaatactggt gcacagctgc tgtgccgatt gtttgctaaa actggtggag gcgatgaaac
     6541 aagaaagtga taagaatttg gaactaacgg cttatgttta taatcctaac attcatcccc
     6601 ggtcggaata tttgtcgaga ttaaaagccg tccaacaaat tgctaaagaa attaaaattg
     6661 aattggtggt ggcagactgg agtccaaaag agtggtttga ggtcaataaa aataaatttg
     6721 aaaagccgga aagatgccac c
//
LOCUS       DAOSHV010000025         6731 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_9882_length_6731_cov_4.805872, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000025 DAOSHV010000000
VERSION     DAOSHV010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 6731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6731
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9882_length_6731_cov_4.805872"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            127..2046
                     /gene="ftsH"
                     /locus_tag="PKZ29_01520"
     CDS             127..2046
                     /gene="ftsH"
                     /locus_tag="PKZ29_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOG37583.1"
                     /translation="MKISEMWQKKEKVKGNKEVEKKDKSDEEMFKNLPPGVKIKKIEI
                     GPKNIIKWLIYIFLFWWMISFLGESLVKSTVNQVSMSEFIDKVKKDEVKKVMVSDNEI
                     VAEMTNEEKLLVTSKEPNISLTEILQREGVDLTKVNVSVNNRQGWKMMGEVLGLVLSI
                     GVPILFIIWILGRQGSGGAGGMFGFGKSTAKLFIKGKQNVTFKDVAGVKEAKDDLMEV
                     VDFLKHPEKYQKMGARAPKGVLLVGPSGVGKTLLAKAVAGEANVPFYSMAGSEFMEML
                     VGVGASRVRDLFGTAKKAGKAIIFIDEIDAIGRMRGGIDGGHGEREQTLNQILVEMDG
                     FEVNTAVVVLAATNRGDLLDPALLRPGRFDRRVMLELPDLEARKDILAIHAKGKPFEE
                     EVNWESVAKSTVGFSGADLENMLNEAAIAAARDNKEKIGRAEIDEAGLKVKLGSEKRR
                     NQSEEDKLMTAYHEAGHAIVNFVEKLDPVSKVSIVSRGMALGFTLVPPQRDIIHQTKG
                     KLIKQMAMAMGGRAAEEIVFGDITTGAASDISHITNIARNMVVEWGMSDLGPINLGPQ
                     IDINDFGRAYMEPSKVSDKMQALVDEEIKKLVGEAHGEAKRVLTKNRLKLDKVAKMLV
                     EKESLGQEEFEELMK"
     gene            2073..4541
                     /locus_tag="PKZ29_01525"
     CDS             2073..4541
                     /locus_tag="PKZ29_01525"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOG37584.1"
                     /translation="MEFVVWHYLRGWKWYWKRFWFNLRRLEHFFSFRVLIKTLLAPWK
                     RMRIVNENAGFDIAKFFENLSFDLISIMIGVIVRLGLVVFFLVLVVIYSGWSVVWFLV
                     WWMLPITGWNYYKLDRRNFGSELKEMTANIRKNPQVAGQIIFESKPGRFILERLEQNM
                     VEIVKKMEVDGDDLVDLDGSSMEKIISWFLGKNGGVETELQKMGMNIEEVILAAKWWD
                     RRQLLGEGKEIDKWELGRPGIGWSLLFGYTPNLDKYSEDLSVKQEFSGHLIGRENVVE
                     RMERSINAGKNILLVGDPGVGKMTVVYAFAEKAITGKLGKELMYKKLVIFDYRMAMAG
                     TEDSDAKKKILSKLMGEAKMAGNIVLVMRDIFRITNAEVEGNDYTDIINNSLEDDRLK
                     IISVVDRVSYERFLASDRRVLKNFEVVEVLPPSKEEALLILMQAVDEVESKNKKIRFS
                     IQAVKQILDGSDRYITDTPFPEKSLELMDLVVENEEINGSLVTKDEVNRVLSEKTGIS
                     LARLTELEKEKLTNLEEIIGRSLIGQKTAVDLVAKSLRSRVVALGNEKKPVGSFLFLG
                     PTGVGKTEMAKVLADIYYGSRENILRFDMAEYLGQEGVERLIGSVVNNRPGLLTKEIK
                     NKPASLLLLDEIEKAPKEIYNLFLTLLDEGYISDSSGEKVVCRHLFVVVTSNAGANFI
                     RENIKKGVRGEELQKKVMEYVQKEGIYSPEFLNRFDGVVVFEPLEKESLMLVAELMLT
                     ELKESLLKRNINLKFDGGVVEKIVRDGYSLELGARQMRRVIELDLGDAIGQAVINGKI
                     GSGDEVVIGVGTINDKFVVGKETI"
     gene            complement(4534..6213)
                     /locus_tag="PKZ29_01530"
     CDS             complement(4534..6213)
                     /locus_tag="PKZ29_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37585.1"
                     /translation="MTSAGTNSLTNHIRSYRSALKSNLEITIHSLKPSYLRLKSLLHP
                     LASDPDEIDFSALIYCLNRLPQVTAKISKIVLGQSPEIYADAGYANLLSWQIVQAHSR
                     RRVAYFNSKTKTLAYLIASVSDIDDLVNILIAFQTEWNKFNHLLHKHYSSFNLLLKDF
                     HTPAFLDKFKLTSDTWQKFFTSLGFDWKQELGYIYHSPLDLKIQLLSASWLNYTKTSQ
                     RWWKNIAATVSSTFHISRQEIYFVSSNTHSLINAITGFPTTHQSQIIDFIHQHHPHLY
                     HHWQKIIDGNTRLNLTDFLYYASRYLLHQPKYQSAYQKLQRQLGIITIPNQEYLDITV
                     QIIPIKNIPASPHLDSRLKITRPSQLKKSPALIFNIDYPLGFAAYHILSEVMQNVRHV
                     NGVYILGKAATLNSEIGDIQIPRLIFDEHTQNSYLFKNCFNNFFPFVNHQGSILTNQR
                     AVSVLGTFLQNRALVNFYLKNNITVIEMESGPYLSAITEGTYDQQAPKNTIVDLNSAP
                     YDVGIINYTSDTPFSSVQNLGDSNLGLSGIEPVTLGSLAILQRIINLEQVK"
     gene            6280..>6731
                     /locus_tag="PKZ29_01535"
     CDS             6280..>6731
                     /locus_tag="PKZ29_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37586.1"
                     /translation="MTERPSAIEFIKQASQEARQKENNQRVGRIAELLVNNAGVLGRT
                     IEKEKSDQVCEAVLGVFGAGYKSVEEMSNTLKNPSATDRQKEMAKENLEVLGKGIFVA
                     MNRTFANFSEEATETITSQMTGEQIDEFVLPDQLKGAGKYVVEADGKAT"
BASE COUNT         2187 a          682 c         2068 g         1794 t
ORIGIN      
        1 gataatcgag aaatgttggt ggagctgagc gggttttagt gagtgcggag taaattagga
       61 gattgtgcgg taattgagaa ggaaaatagg gtagggtgag ggtagagtag atgatataat
      121 cagagaatga aaataagtga aatgtggcag aaaaaagaaa aggtaaaagg gaataaagag
      181 gtggagaaaa aagataagtc cgatgaggaa atgtttaaaa atctgccgcc aggggtaaaa
      241 ataaaaaaaa tagaaatagg accaaaaaac ataattaagt ggttgatata tatatttttg
      301 ttttggtgga tgatatcttt tttgggggaa tcgttggtaa aatcgacagt aaatcaggtg
      361 tcgatgtcag aatttataga caaggtcaaa aaagatgagg tcaaaaaggt gatggtgtcg
      421 gacaatgaaa ttgtggcgga gatgactaat gaagaaaagc tgctagtgac ctcgaaggag
      481 ccaaatattt ctttgacgga aatattgcaa agagaagggg tagatttaac caaagtaaac
      541 gtaagtgtta ataatcgcca aggatggaag atgatgggag aagtgctagg gttggtgttg
      601 agtattggag tgccgatttt gtttattatt tggattttgg ggagacaagg gagtggtgga
      661 gccgggggaa tgtttggttt tggtaaatcg acagcaaaat tgtttataaa agggaagcaa
      721 aatgtgacgt ttaaagatgt ggcgggggta aaagaggcca aagatgattt gatggaagtg
      781 gtggattttt tgaaacatcc ggaaaaatac caaaaaatgg gagccagggc gcctaaggga
      841 gtgttgttgg tgggaccaag cggggtggga aaaacattgt tggccaaggc ggtggcagga
      901 gaggcaaatg tgccgtttta ttcgatggca ggaagcgagt ttatggaaat gttggtggga
      961 gtaggggcct caagagtcag ggatttgttt gggacggcta aaaaggcggg gaaggcgatt
     1021 atttttattg atgaaattga tgctattggg cggatgaggg gcggaattga cggcggtcat
     1081 ggagaaagag aacagacgtt aaaccaaatt ttagtggaaa tggacggatt tgaggtcaat
     1141 acggcagtgg tggtgttggc ggcgaccaac aggggagatt tgttggaccc agcattgttg
     1201 agaccgggtc ggtttgaccg aagagtgatg ttggaattgc cggatttgga agcaagaaaa
     1261 gatattttgg caattcatgc caaaggcaaa ccgtttgagg aagaggtaaa ttgggaatcg
     1321 gtggcaaaaa gcacagtggg attttcgggg gcggatttgg aaaatatgct gaacgaagcg
     1381 gcgattgcgg ctgccagaga caataaggaa aaaattggtc gagcagaaat tgatgaggcg
     1441 ggattaaagg ttaagttggg gtcggaaaaa agacgaaatc aaagtgagga agataaattg
     1501 atgacggctt atcatgaagc ggggcatgcg attgttaatt ttgtggaaaa attagatccg
     1561 gtgtcaaagg tgtcgattgt atctcgggga atggctttgg gatttacctt ggtgccgcca
     1621 cagcgggaca taattcatca aacaaaaggg aaattgatta agcaaatggc gatggcgatg
     1681 ggaggtcgag cggcagagga gattgtgttt ggggatatta ctactggagc ggcttcggat
     1741 atctcccaca taactaatat agccagaaat atggtggtgg agtggggaat gagcgatttg
     1801 gggccgataa atttgggacc gcaaattgat attaatgatt ttggcagggc ttatatggag
     1861 ccaagcaagg tatcggataa aatgcaggct ttggtggacg aggagattaa aaaattagtg
     1921 ggggaggctc atggtgaggc aaaaagggtg ctgactaaaa acagactaaa attggataag
     1981 gtggccaaaa tgttggtaga gaaagaaagc ttgggacagg aggaatttga agagttgatg
     2041 aaataaatta agtagcgatg gtatcatcaa ttatggaatt tgttgtttgg cattatttaa
     2101 gagggtggaa gtggtactgg aagcgatttt ggtttaattt gaggaggttg gaacattttt
     2161 tttcttttag ggtgctgatt aagacattat tggcgccttg gaaaagaatg agaattgtta
     2221 atgaaaatgc tggttttgat attgctaaat tttttgaaaa cttgtctttt gatttgatct
     2281 caataatgat cggagtgatt gtgaggttgg ggctggtggt gttttttttg gtattggtag
     2341 ttatttatag tggctggtcg gtggtttggt ttttggtttg gtggatgcta ccaataactg
     2401 ggtggaatta ttacaagttg gatcggagaa attttggcag tgagctaaaa gagatgacgg
     2461 ctaatattag aaaaaatcct caagtggcag gccaaattat ttttgaaagc aaaccgggaa
     2521 gatttatttt ggagagacta gagcaaaaca tggttgagat agttaaaaaa atggaggtag
     2581 acggggatga tttagttgat ttggacggaa gcagtatgga gaaaataata agttggtttt
     2641 tgggtaaaaa tggcggagtg gaaacagaac tgcaaaaaat gggaatgaat attgaagaag
     2701 taattttggc ggctaagtgg tgggatagga ggcagttgtt gggtgagggg aaagaaattg
     2761 ataaatggga gctggggaga ccgggaattg gttggagttt gttatttggc tatactccca
     2821 atttggataa atacagtgaa gatttaagtg taaaacagga attttccggt catttgatcg
     2881 ggagagaaaa tgtggttgag agaatggaga ggtcaattaa tgcgggaaaa aatattttgt
     2941 tggttgggga tcccggggtg ggaaaaatga cagtagttta tgcttttgcg gagaaggcaa
     3001 ttacgggaaa actaggaaaa gagttgatgt ataaaaaatt agtgatattt gattatcgga
     3061 tggcgatggc gggaacagaa gatagtgatg ctaaaaaaaa gatattatca aaattgatgg
     3121 gtgaagcaaa aatggcagga aatattgtct tggtgatgag ggatattttt cgaattacca
     3181 atgccgaagt tgaaggaaat gattatacag atatcataaa caactcatta gaagatgaca
     3241 ggttaaaaat aatttcggtg gttgataggg tcagttatga aagatttttg gcaagtgaca
     3301 gaagggtttt gaaaaatttt gaggtagttg aagtgttacc gccgagcaaa gaagaggcgt
     3361 tgttaatttt aatgcaggca gttgatgagg ttgagtcaaa aaataaaaaa ataagatttt
     3421 cgatccaggc ggttaaacaa atattggacg gaagcgatag atatataacc gataccccat
     3481 tcccggaaaa atctttggag ctgatggatt tggtggtaga aaatgaagaa ataaacggtt
     3541 ctttagtaac gaaagatgag gtgaataggg ttttgtcgga gaaaacaggt atttctctgg
     3601 ctagattgac tgagttagaa aaagaaaagt taactaattt agaagaaata ataggacgaa
     3661 gtttgattgg gcaaaaaacg gcagtagact tggtggctaa gagtttgagg agccgggtgg
     3721 tggcattggg gaatgaaaag aagccggtgg gatcattttt gtttttgggg ccaacagggg
     3781 tgggaaaaac agaaatggct aaagtattgg cagatatcta ttatggttct agggaaaata
     3841 ttttgagatt tgatatggcg gaatatttgg ggcaggaagg ggtagagaga ttgattgggt
     3901 cggtggttaa taatcggccg ggtcttttga caaaagaaat taaaaataag ccagctagtt
     3961 tgcttttgct tgatgaaatt gaaaaggctc caaaagagat atacaactta tttttgacct
     4021 tgctggacga gggttatatt agtgattcaa gcggggaaaa agtagtttgt cggcatttgt
     4081 ttgtggtagt aacttctaat gccggggcaa attttattag agagaatatt aaaaaaggcg
     4141 ttaggggtga ggagctacag aagaaagtga tggagtatgt tcaaaaagag ggaatttatt
     4201 caccggagtt tttgaacaga tttgatgggg tggtggtgtt tgagccattg gaaaaagaga
     4261 gtttgatgtt ggtggcggag ttgatgttga cagaattaaa agaaagttta ttaaagagaa
     4321 atattaattt gaaatttgac ggtggggtgg tggagaaaat agttagagat ggttatagtt
     4381 tagagttggg ggcaaggcaa atgcgacggg taatagaatt ggatttgggg gatgcgattg
     4441 gacaggcggt gataaatggt aagattggtt cgggagatga agtggtgata ggagtgggta
     4501 caataaatga taagtttgta gtaggaaaag aaactattta acttgttcaa ggttgatgat
     4561 tcgttggagg atggcgaggg agccgagggt aacgggttcg ataccactta gaccaagatt
     4621 agaatcacca agattttgaa cagaagaaaa aggggtgtcg gaagtgtagt tgataatacc
     4681 aacgtcgtaa ggagcggaat tgagatcaac aatggtattt ttgggggctt gttgatcgta
     4741 agtaccttcg gtgatggcag agagataggg accggattcc atttcgataa cggtaatatt
     4801 gtttttgaga taaaaattga ctaaagctcg attttggagg aaggtgccaa ggacagaaac
     4861 ggctcgttga ttggttagaa ttgaaccttg atggttgaca aaaggaaaaa agttgttgaa
     4921 acaatttttg aaaagatagg agttttgggt gtgttcgtcg aaaattagac gaggaatttg
     4981 aatatcgcca atttcgctgt tgagagtggc ggctttgcca agtatgtaga caccattgac
     5041 atggcggaca ttttgcatga cttcgctaag aatgtgatag gcggcaaaac caagaggata
     5101 gtcaatattg aaaataagag cgggggattt tttgagttga gaaggacggg taattttgag
     5161 acgggaatca agatgggggg aggcaggaat atttttgata ggaattattt ggacggtaat
     5221 gtcgagatat tcttgattgg ggatagtaat aatgccaagt tggcgttgga gcttttgata
     5281 ggcagattga tatttaggtt gatggagaag ataacgagaa gcatagtaaa gaaagtcggt
     5341 gagattaagg cgggtgttgc catcaataat tttttgccag tggtggtaga gatgaggatg
     5401 atgttggtgg ataaaatcaa taatttggga ttggtgggtg gtaggaaagc cggtgatggc
     5461 attgataaga ctgtgagtgt tgctggaaac aaaatagatt tcttgacgag aaatgtgaaa
     5521 agtggaagag acagtagcgg cgatattttt ccaccatctt tgggaggttt tggtgtaatt
     5581 aagccaggaa gcagatagaa gttggatttt tagatcaaga ggagagtggt agatataacc
     5641 aagttcttgt ttccagtcga agccaaggga ggtgaaaaat ttttgccagg tatcggaggt
     5701 gagtttgaat ttgtccaaaa aagctggggt gtgaaaatct ttaagaagaa gattgaaaga
     5761 tgaatagtgt ttatgaagaa gatgattaaa tttgttccat tcagtttgga aggcaataag
     5821 aatattgaca aggtcatcaa tatcggagac agaggcaatg agataggcaa gagttttagt
     5881 tttggaattg aagtaagcga cacgacggcg gctgtgagct tggactattt gccaagaaag
     5941 gaggttggcg taaccggcat cggcataaat ttccgggctt tgaccaagaa cgattttgga
     6001 gattttggcg gtaacttgag gaagtcgatt gagacaataa attagggcgg aaaaatcgat
     6061 ctcatctggg tcggaagcga gagggtgaag gagtgatttg aggcggaggt aagaaggttt
     6121 gagagagtga atagtgattt caagattgga tttgagagcg gaacggtagg aacggatatg
     6181 gttggtaagg gaattagtac cggctgaagt catagatgat taattttagc agaagctata
     6241 gattagtaaa aaagttggga aattggtata atttggggta tgacagaacg accatcagca
     6301 attgaattta tcaaacaggc tagtcaagag gctagacaga aggaaaataa tcagagagtg
     6361 ggtaggattg ctgaattgtt ggtgaataat gcgggagtgt tgggaagaac aattgagaaa
     6421 gaaaaatcag atcaggtatg tgaagcggtg ttgggagtgt ttggggcagg gtataaatca
     6481 gtagaggaga tgagcaatac tttgaaaaat ccgagcgcaa ccgacaggca gaaggaaatg
     6541 gcaaaagaga atttggaagt tttaggaaag gggatttttg tagcaatgaa cagaactttt
     6601 gccaactttt cggaagaagc cacagagacg attacgtctc agatgactgg agaacagata
     6661 gatgagtttg tattaccgga tcagttaaaa ggggcaggaa agtatgtggt ggaggctgat
     6721 ggaaaggcga c
//
LOCUS       DAOSHV010000026         6723 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_9896_length_6723_cov_6.090732, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000026 DAOSHV010000000
VERSION     DAOSHV010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 6723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6723
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9896_length_6723_cov_6.090732"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            159..2798
                     /gene="ppdK"
                     /locus_tag="PKZ29_01540"
     CDS             159..2798
                     /gene="ppdK"
                     /locus_tag="PKZ29_01540"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013006994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HOG37587.1"
                     /translation="MNKYVYFFGNKKAEGDKEMKFILGGKGANLAEMTNLGIPVPAGF
                     TISTEVCKYYYDNGKKYPVELKGQIDEGLKKLEENTGKKFGDIKNPLLVSVRSGAAAS
                     MPGMMDTILNLGLNEKVVAGMVEKTGNARFVWDSYRRFIQMYSNVVENLEHEKFEHVI
                     ASVKAKKGIKEDTQMTAEDWQEVVKGYKEIYKIEKGREFPENPEDQLNGAIGAVFGSW
                     NNPRAIIYRQLNKIDESKIFGTAVNIQSMVFGNMGDDCGTGVAFTRNPSTGENKAYGE
                     YLMNAQGEDVVAGIRTPKHLESLKEDNETAYKELLEIFERLEKHYKDMQDVEFTIENG
                     KLFILQTRNGKRTAPAMVKVAVDLVKEGMIDEKTALTRVEADKLDQLLHPTLDSQQKT
                     NFKVIAKGLPASPGAAYGPVVFSAEEAVKSAEAGKKAILVRLETSPEDIAGMNAAAGI
                     LTARGGMTSHAAVVARGMGRCCVSGCESIAVSEEMKMFKTGETEVKEGDIITLDGSTG
                     EVFLGQVPTVEPELSGDFGQLMKWADETRKLGVRTNADTPKDAKTAREFGAEGIGLTR
                     TEHMFFEEDRIFAIRQMIISDTVEQREAALAKIFPMQKNDFKQLFEIMKGLPVKIRLL
                     DPPLHEFVPKEDKDIEALAKEMGISFEKLKDKVESLHEANPMMGHRGCRLGITYPEVF
                     QMQVKAIMAAACELKKEQKIEIVPEIMIPLIADVEELKWLKNKLVPVIEETMKSYGVE
                     LKYEIGTMIEIPRATVTADEIATEADFFSFGTNDLTQMTYGFSRDDAGKFIKDYLREK
                     IMNEDPFKSVDQKGVGKLMEMAVKLGRSSNKDLNIGICGEQGGDPETVKFCHRIGLNY
                     VSCSPYRVPIARLAAAQAVVEGK"
     gene            3113..3394
                     /locus_tag="PKZ29_01545"
     CDS             3113..3394
                     /locus_tag="PKZ29_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37588.1"
                     /translation="MDWQLSVTAFGCGEGRRTETWPERIEVGELMLAKKSWQDKAPDF
                     IPWKEIQRIGGCKVSLIYADNQYKFTVAGRSPVEVKKITEVIDWQMKRS"
     gene            3790..4218
                     /locus_tag="PKZ29_01550"
     CDS             3790..4218
                     /locus_tag="PKZ29_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37589.1"
                     /translation="MKSKPKKLMVELLKNLLEKLFRAKTIFDGEKLKLTASVPVGLGG
                     EMNLMFWEDVDGAPVLVLNVPPLPWGVDEEVYVLLKELLEWRYGELPKSVKYGSAAFR
                     GHKFYGWKPNDFNLMYVYLWDKTGWSEARLNELILKGETK"
     gene            4215..4571
                     /locus_tag="PKZ29_01555"
     CDS             4215..4571
                     /locus_tag="PKZ29_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37590.1"
                     /translation="MTQEVVERVIAKYGGATWLKNMVPETLFRFLKVEDGGRTETIMV
                     EIDGESGWMIFVDKSISKQRRVLAVFYEKGKCPSFYPGRLKYDVSLGLILVHELSEFF
                     DPRVADFGKKVFNLFL"
     gene            4855..5223
                     /locus_tag="PKZ29_01560"
     CDS             4855..5223
                     /locus_tag="PKZ29_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37591.1"
                     /translation="MILKLKDFFQRNFGVQPKMRLNSSQILIAATVEIEHWVSVWSDG
                     GENFCLMGNGQIRVHELMNLTNDLMKSLSLTESSIGGHCSHDLWAVPMAKERAASINQ
                     VEVCRIKQESENLIGGAYDR"
     gene            5213..5512
                     /locus_tag="PKZ29_01565"
     CDS             5213..5512
                     /locus_tag="PKZ29_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37592.1"
                     /translation="MTDESLFQVVELVDTLMRTRIPDARITINPEKLPGKVANKTHRT
                     LFLRTEQNWVEIWIEEGFTGEEIKGIKDFFKGKAKLTFESGPLLRWRWKPELEKE"
     gene            5515..5724
                     /locus_tag="PKZ29_01570"
     CDS             5515..5724
                     /locus_tag="PKZ29_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37593.1"
                     /translation="MSGCFFVSPAVFHWFHFDNEVKERSLETGILTIKGTFRNKDVTV
                     EVKNGLARVMADDGILPRLEAALQA"
     gene            complement(5771..6637)
                     /locus_tag="PKZ29_01575"
     CDS             complement(5771..6637)
                     /locus_tag="PKZ29_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37594.1"
                     /translation="MFDLSLSPSQIEFVVRPGSGLTQAYQVVNNSDQSITLNTKILPF
                     LPSGDTGSVTYHQISPNPSIIFSLNNADLQLGQPFTLPPKSQKQLVLKINTLSQAKSD
                     DSYFTFFVYQTDQTSANDGNITQTTGQIGSHILLSLSSTETPATAGQIQKFSTKPLIK
                     DVFFTPISFTAQVNNHTDYFFKTAGKIIISKNSQVVTEIKLDDNNVLAHHSRQISCGQ
                     QTCTLSPPLWPGSYSASLEFDPHLNLKPVSLSFFVFPFSPLLIIVTVFLAIFAFTKIK
                     SKFSKKKYRGVN"
BASE COUNT         2116 a          899 c         1907 g         1801 t
ORIGIN      
        1 gtcggcgggc gaagcaagag gggaggaaaa agtctccctt gtcaaaggga gatttggagg
       61 gattgggaaa tagaagagag taaagaggtg gcttgaaaaa gaaaattttt tgggataaag
      121 tagagcatta tctttttaga aagttaaaaa aggaaaatat gaataaatat gtctattttt
      181 tcggcaataa aaaagctgaa ggcgataagg aaatgaaatt tattttgggg ggcaaaggag
      241 ctaatttggc agagatgact aatttgggaa ttccggtgcc ggcagggttt acaatttcga
      301 cggaagtttg taaatactat tatgataatg gcaaaaaata cccagttgag ttaaaaggac
      361 agattgatga aggtttaaaa aaattggaag aaaatacagg caagaaattt ggggatatca
      421 aaaatccgtt gttggtgtcg gttcgatcgg gggcggcagc atcgatgccg ggaatgatgg
      481 atacaatttt gaatttggga ttaaatgaaa aagtggtggc gggcatggta gaaaagaccg
      541 gcaatgccag atttgtgtgg gattcttacc gaagatttat tcagatgtat tctaatgtgg
      601 tggaaaattt ggagcatgaa aaatttgagc atgtgattgc ttcggttaaa gccaaaaagg
      661 ggattaaaga agatacccaa atgacggcgg aggactggca ggaagtggtt aagggctaca
      721 aagaaattta taaaattgaa aaaggcagag agtttccgga aaatcccgaa gatcaattga
      781 acggcgctat cggcgccgtg tttggttcct ggaataatcc tcgggcaatt atctatcggc
      841 agttgaacaa aattgatgaa agtaaaattt ttgggacggc ggttaatatc cagtcaatgg
      901 tgtttggcaa tatgggggat gattgtggca ctggagtggc gtttacaaga aatccgtcaa
      961 ctggagaaaa caaggcttat ggcgaatatt tgatgaatgc tcagggtgaa gacgtggtgg
     1021 caggaatccg gacgccaaaa catttagaat ctcttaagga ggataatgaa acggcttata
     1081 aggagctgct ggaaatattt gaacggctgg aaaaacacta taaagatatg caggatgtgg
     1141 agtttacgat tgaaaatggt aagttgttta ttttgcaaac ccgaaacggc aagagaacag
     1201 cgccggcaat ggtaaaagtg gcagtggatt tggttaaaga ggggatgatt gatgaaaaaa
     1261 cagctttgac tcgggtggaa gccgataaac tggatcaatt gctgcatccg actttggata
     1321 gtcagcaaaa aactaatttt aaagtaattg ccaagggttt gccggccagt ccgggagcag
     1381 cttatggtcc ggtagtgttt agcgcagaag aggcggtaaa atcggcggaa gcaggcaaga
     1441 aggcaatttt ggtaagactg gaaacatcac ctgaggatat tgcgggaatg aatgcagccg
     1501 caggaatctt gacggccagg ggagggatga cctctcatgc ggcggtggta gctcggggaa
     1561 tgggtcggtg ctgcgtgtcc gggtgtgaat caatcgcggt atcagaagaa atgaaaatgt
     1621 ttaagaccgg tgaaacagaa gttaaagaag gagacattat cactttggac gggtcgacgg
     1681 gagaagtatt tttgggtcag gtgccgacgg tagagccgga gttgagcggg gattttggtc
     1741 agttaatgaa gtgggctgat gaaacccgca aactgggagt cagaaccaac gctgatacgc
     1801 ccaaggatgc taaaactgcc cgggagtttg gagctgaagg tatcggtttg acccgaaccg
     1861 agcatatgtt ttttgaagaa gacagaattt ttgctatccg gcagatgatt atttcggata
     1921 cggtggaaca gcgggaagcg gctttggcta aaattttccc gatgcagaaa aacgatttca
     1981 aacaattgtt tgaaattatg aaaggtctac cggttaaaat ccgactgttg gatccgccgc
     2041 tgcatgaatt tgtgcccaaa gaagacaaag atattgaagc tctggccaaa gaaatgggga
     2101 tttcatttga aaaactcaaa gacaaggtgg aatcgctgca cgaggccaat ccaatgatgg
     2161 gtcatcgggg ttgtcggttg gggattacct atccggaagt gtttcaaatg caggtaaaag
     2221 ctattatggc agcagcctgt gagttgaaaa aagaacaaaa gattgaaatt gtgccggaaa
     2281 ttatgattcc gctaattgct gatgtggaag aattgaagtg gctgaaaaat aaattggtgc
     2341 cggtgattga agaaacgatg aagtcgtatg gagtggagtt gaaatatgaa atcggaacca
     2401 tgattgaaat tccccgagca acagtaacgg cagacgaaat tgccactgag gcagatttct
     2461 tttcgtttgg aactaatgat ttgacccaaa tgacctatgg tttctctcga gatgatgcgg
     2521 gtaagtttat taaggattat ttgagagaaa aaataatgaa cgaagatccg tttaagagtg
     2581 tggatcaaaa aggggtggga aagttgatgg agatggcagt caaattaggt cgaagcagca
     2641 acaaggactt gaatatcggt atctgcggtg agcagggcgg tgatccggag acggtaaaat
     2701 tctgtcaccg aatcggactt aattatgttt cctgcagccc atatcgggtg ccgattgcca
     2761 gactggcggc ggctcaagca gtggtggaag gtaaatagat atcagatgat taagttcata
     2821 aagtaaaaac attaggatca accctaatca tcaatttttc cactggaaaa tgagtctgcg
     2881 atgcaggctc aaatacgttc caaaatgatg atttcgggtt gattggggat taaattaagt
     2941 agatgtcggt tgcaaaacct acctaatgat ttgtgggtaa aaattctgaa ttggggagag
     3001 gattttatcc tataatcttg attaaattaa agttttttgg taaaatagcc ccataaatta
     3061 gtttccgtga gcggaattta atttatagaa cattgacaga ggagggtttg agatggattg
     3121 gcagttatca gtgacggcgt ttggatgtgg tgagggcaga aggacagaaa cttggcctga
     3181 acggattgaa gtgggagagt taatgctggc gaaaaaaagc tggcaagaca aagcgccgga
     3241 ttttatcccc tggaaggaaa ttcaaagaat tggaggatgc aaggtaagcc tgatttatgc
     3301 cgataatcaa tataagttta cggttgccgg cagatccccg gttgaggtaa aaaagataac
     3361 ggaggtgatt gattggcaaa tgaaacggag ttgatttgaa gctgcagatg aaaatgacaa
     3421 aacatttggt tttaggagga aacatggata aacaggaaaa aaaaacccta ccaaagagac
     3481 agaaacagcc tggtttggta atatttggcg ggatgaaccc agccaagacg tagatttgac
     3541 caaacagctg gctgacgact gactcattta ccccggaggt agattttagc taaggctaga
     3601 atttattaaa ctggggtatt ttttttgaaa aaattgggta agtagtttat ttgtattata
     3661 ggttatttaa tggtataatt tgtccgattt tcagaataaa tttaaccaag agagcttggt
     3721 ttttaaatat tttttttgga attcggaact ttaacaataa gctcaaggat ttttggagag
     3781 gagaagtaaa tgaaaagtaa accaaaaaaa ctgatggtgg aactgctaaa aaatctgtta
     3841 gaaaaactat ttcgggccaa aacaattttc gacggagaaa agctaaagtt gacggcgtcg
     3901 gtgccggttg gtcttggtgg agaaatgaat ttgatgtttt gggaagatgt agatggggcg
     3961 ccggtattag tgttaaatgt gccaccttta ccttggggag ttgacgagga agtttatgtg
     4021 ttgctaaaag aactattgga atggaggtac ggtgaattac ctaagtcggt aaagtatggc
     4081 tcggcagcat ttaggggcca taaattttat ggctggaaac caaatgattt taatctgatg
     4141 tatgtttatc tttgggataa gaccggttgg agcgaggcaa gacttaatga gttgattttg
     4201 aaaggagaga caaaatgacc caggaagtgg ttgaaagagt tattgccaaa tatggaggcg
     4261 ctacttggtt aaaaaacatg gtgccagaaa cgttgtttag gtttttgaaa gtagaggacg
     4321 gagggagaac tgagacaata atggttgaga ttgatggcga gagtggttgg atgatctttg
     4381 tagacaaatc gatttctaaa cagaggcggg tgttagcagt tttttacgaa aaaggaaaat
     4441 gcccaagttt ttatccggga cgattaaaat atgatgtgag tttgggtttg atattggtgc
     4501 atgagttgtc ggaattcttt gatcctcggg tggctgattt tggcaaaaaa gtgtttaatt
     4561 tatttttgta aaagagttct tgagtttgac ccgggctggg aataggattg cccaaagtgg
     4621 taaatcttat tgtcagctcg ggatatttgt ttctatagac aaaaactata ctgacgagtc
     4681 ttgggggaga cgaaaaacaa atatatctta taatattgat ttttgatgat ttttgtggta
     4741 gaataggggg ttgatggtaa attaatggtc actaagcgtg gctttttgaa atatttttca
     4801 tcaaagttct ttaaaaaaga aggaggggag gtgccttgag tggttgatat tagtttgata
     4861 ctgaaactta aagatttttt tcagagaaat tttggagttc agcctaaaat gaggttaaac
     4921 agtagtcaaa ttttgatagc ggctacggta gagattgaac attgggtgtc ggtttggtct
     4981 gatgggggcg aaaatttttg tttaatgggt aatggtcaga ttagggttca tgagttgatg
     5041 aacttaacaa atgatttaat gaaaagtctt agtctgaccg agtcgagtat tggcggtcat
     5101 tgcagtcacg atttatgggc ggtgccaatg gcaaaagaaa gggcggcgtc gataaaccaa
     5161 gtggaagttt gtcggatcaa acaggaaagt gaaaatttaa taggaggggc atatgacaga
     5221 tgaatctctg ttccaggtgg tggagttggt ggatacttta atgaggacta ggatacctga
     5281 tgcaaggata actatcaatc cagagaaact tccgggtaag gtagcaaaca agacacatcg
     5341 aacgttgttt ttgagaaccg agcaaaattg ggtagagatt tggattgaag aaggctttac
     5401 tggcgaagag atcaaaggaa ttaaggattt ttttaagggt aaggctaaat taacgtttga
     5461 atccggtcct ctgctgaggt ggcggtggaa gccagaacta gaaaaggagt gactatgagc
     5521 ggttgttttt ttgtcagtcc ggcggtattt cactggtttc actttgataa tgaggtcaag
     5581 gaaaggagct tggaaaccgg aattctgacc ataaaaggaa cttttaggaa caaggacgtg
     5641 acagtggaag tgaaaaatgg tttagccagg gtgatggctg atgacgggat tttgccccgt
     5701 ttggaagcgg ccctgcaggc ttaattatac cggagggtag acttgataat taaagcgtaa
     5761 gctttattaa tcagtttact ccccggtatt tttttttgga aaatttagat ttgatttttg
     5821 tgaaagcaaa aatagccaga aagacggtaa caataattag aagaggagag aagggaaaaa
     5881 caaaaaaaga aagggaaacc ggtttgagat tgagatgggg atcaaactcg agggaagcag
     5941 agtaggatcc aggccaaaga gggggagaaa gggtgcaggt ttgttgacca cagctaattt
     6001 ggcgggagtg atgggcaagg acgttgttgt cgtcaagttt gatctcagtg acaacttggg
     6061 aatttttgga aataataatt ttgccggcgg ttttgaaaaa atagtcggta tgattgttaa
     6121 cctgagcggt gaaagaaata ggggtaaaaa aaacatcttt gataaggggt ttggtggaaa
     6181 atttttggat ttgacccgca gtggctggag tttcggtgga agaaagacta agaaggatgt
     6241 gtgagccgat ttgaccggta gtttgggtaa tgttgccgtc gttagcagag gtttggtcag
     6301 tttgataaac aaagaaagta aaataggaat cgtctgactt ggcttgagag agagtgttta
     6361 tttttagaac taattgtttt tgggatttgg ggggcagggt aaagggctga cccagctgga
     6421 gatcggcatt gttgagagaa aaaataatgc tgggattggg tgagatttga tgataggtga
     6481 cagagccggt gtcgccgctg gggagaaaag ggagaatttt ggtgttaagg gttatggact
     6541 ggtcggaatt gttgacgact tgataggctt gggtcagacc ggaaccaggg cggacgacga
     6601 actcaatttg ggagggagaa agagataggt caaacataat ttagtttagc aggcctatga
     6661 agttcatcaa gtttatcccg atgtcacttc gttcatcggg attgggtttt taggcaaaat
     6721 tat
//
LOCUS       DAOSHV010000027         6001 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_11645_length_6001_cov_4.842583, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000027 DAOSHV010000000
VERSION     DAOSHV010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 6001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6001
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11645_length_6001_cov_4.842583"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(212..2371)
                     /locus_tag="PKZ29_01580"
     CDS             complement(212..2371)
                     /locus_tag="PKZ29_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37595.1"
                     /translation="MAIWSMRIQKKGRLEAFLGETLYGYSREESPDLSMMGKIFNNPD
                     EADRQRRLKKFKEELLYPEALLINKDSVPNSYFELQLRIARQRGQGADYDRVGIRTAD
                     DFSPEARKKAGETIYRDQKKSLDVWADYLSSPEAGYPTWFKFYVLKSVIKMGVYDKDK
                     KEFSKRTKDTTTIFPDLNREALAYCYDVITKYYLKREGHVSEELRSILESTSFSRIYA
                     FAIDEVTPASKENKEKTRGEWIKFDQGGDHIPLYKSLQGHGTGWCTAGEEVAKDQLSR
                     GDFYVYYTEDEKGNRTIPRIAIRMEEGEVFEVRGINPGQNLEPNMLEIAKNKYQQLPG
                     GSSFEKKDRDMERLTLIDGKVKAGKELTRDELRFLYEINQSINGFGYEKDPRVGVVRS
                     ARNQVSDINVALEDIEVVESGVDLSYLTSLDGINLPRVINGDLNLSGLVSIESLKLPQ
                     RVNGVINLSGLSLDEIRKLPEMINGGLVLSIPLAEYDFFGNLNVPEMSTEGLRLPLVV
                     DGDFCFDGLLSAKGVDLPKVVGGNLDLSHLKTADGLKLPETISGSIFLPELKSADGLV
                     LPKIINGGLSLDSLESISGLELPDIINGGISLRGLKSADGLVLSGVVGGGIDLGGLTS
                     AQGLVLPEVIKNGDLFLFGLQSAQGLELPKVIEGELCLSTWVEVDKLPKKVRSIRFFS
                     KTQMSSEYRQNLVDYSKEHQIEVYVSYDDETPWVRIN"
     gene            2694..3830
                     /locus_tag="PKZ29_01585"
     CDS             2694..3830
                     /locus_tag="PKZ29_01585"
                     /inference="COORDINATES: protein motif:HMM:NF014872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOG37596.1"
                     /translation="MDTNKLVSDLTAQFPDLKIYYQHSLAPYTTVKIGGPADVFIHST
                     SSTQLTEILKYLSSVDSIFSARKALKDSDEGASTVKNIIKPNITILGNGSNTLISDSG
                     LRGIVIKNSSDQFTILDSVSPATPASTKISAHRTENDPAKYLNFSQIDYDESDQPQVL
                     VKIDSGCNLPSTINRLINAGVTGLQWFGFIPGTIGGAVYGNIHGGTHHFSDFIKEVEV
                     FDLNTGQIKHIPKTNLSWDYDHSSFQSQPNLIILSVTLSLYRGDTARAKAAVQAWITQ
                     KSAVQSMNSLGSVFQNPPLAICQKNWGEQKSAGWIIDTQLNLKGAQIGDAQISPKHAN
                     FMINQSQATATDYLALINLVQTQLRQKFNFQFDLEIKLLGKFTH"
     gene            complement(3977..4369)
                     /locus_tag="PKZ29_01590"
     CDS             complement(3977..4369)
                     /locus_tag="PKZ29_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37597.1"
                     /translation="MTSVRVQVVTKPNRHDILFPSQDLTYGSIVSVDDKTLELMKAIG
                     VVFADGLDSARVLVGTIDVSAVKLIEMSRLLSFDKRSFPIYLVDQADNFLGLWQNGFW
                     FDDRNANLDRIGECLEGVAMSPRPICLA"
     gene            complement(4387..4974)
                     /locus_tag="PKZ29_01595"
     CDS             complement(4387..4974)
                     /locus_tag="PKZ29_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37598.1"
                     /translation="MEFDHLRVIRFYPKAGKKIFRGEGMLATEKGLFSKRTDDLTQRW
                     ERFKKGVIWIECDGGVCYTLCFPTGNGYFKYFILPDVMSVRMVEMVKMVRLESDNVIQ
                     GRPMDQILHQMGAEVKVDGNFCIYVFTRDKAKSINPFELMNEKERSRALTVGLPIVLV
                     DEKGVVQGYYQSGNGLVMENTDHRVRYTVMPVEMG"
     gene            complement(5109..5576)
                     /locus_tag="PKZ29_01600"
     CDS             complement(5109..5576)
                     /locus_tag="PKZ29_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37599.1"
                     /translation="MKAISVRVKDRKGYLTVWFPGLSVSAYTTKVELRGRLSQYEKDI
                     KANKSHIQKMDEIALIQDSFVNYLESVSHKQILPDGMVVYTIKNPKSLSKVDFSVITN
                     YKAAVRPIYFVDDKGDLAALWVDGKFWTKENPEFDLYLPGFRMTKFDDRLSIV"
     gene            complement(5675..>6001)
                     /locus_tag="PKZ29_01605"
     CDS             complement(5675..>6001)
                     /locus_tag="PKZ29_01605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HOG37600.1"
                     /translation="QVRACAGEPTVTNEEVKKDINKFKPDVLLVAYGMKRQEMWIEEN
                     LKKIDAGVVMGVGRSFDYYSGDLKRAPKVWRKIGMEWLYSLIKEPKRIKRQLALPKFV
                     GRVLRG"
BASE COUNT         1773 a         1588 c          842 g         1798 t
ORIGIN      
        1 aaacacccgc attctaccaa cttatattca cccgcctgag attcttgaat attctcagac
       61 ctgagaaccg agatctgaga actgagatct gagaactgag atctaacatc caaaaccgcc
      121 caaccagtgt tagccaatcc cggatcaatt cctaaaatca taactcaatt ataacaatca
      181 gttacccgct aaccgcattt ttaaaaaagt tttaattaat tcgcacccaa ggagtttcat
      241 catcataaga tacataaact tcaatttgat gttcttttga ataatctaca agattttgtc
      301 gatactcaga tgacatttgt gttttagaaa aaaaccgaat agacctgacc tttttaggca
      361 acttatcaac ttctacccaa gtagagagac aaagttctcc ttcaatcact ttaggtaatt
      421 ccaaaccttg agctgactga agaccaaaca aaaaaaggtc accgtttttt ataacctctg
      481 gtaacaccaa tccctgagca gacgttaaac ctcccaaatc aatcccaccg ccaactaccc
      541 ccgataacac caatccgtcg gcagacttaa gcccacgcaa agaaataccg ccatttataa
      601 tatctggcag ttccaaaccg ctaattgatt caaggctatc caaactaaga cccccattta
      661 ttatttttgg taacaccaat ccgtcggcag acttaagctc aggtaaaaaa atacttccac
      721 ttattgtctc aggtaatttt agtccgtcgg cagttttaag atgggataaa tcgagattgc
      781 cacctacaac tttaggtaaa tcaactcctt ttgcagacag aagtccatca aaacaaaaat
      841 ctccatccac caccaagggc aatctcaaac cttccgtaga catctcggga acattcaaat
      901 tcccaaaaaa atcatactca gccaacggga tagataagac gagaccgcca tttatcattt
      961 ctggcagctt tctgatttca tctagtgaaa gacctgacag gttaatcacc ccattcaccc
     1021 tttgtggtaa tttcaaactt tcaatcgaaa ccaaaccaga taaatttaaa tcaccgttaa
     1081 taaccctggg taaatttatc ccatccaaag acgttaggta acttaaatca actccagact
     1141 caaccacttc aatatcctca agagcaacat ttatatcaga tacttgattc cttgccgacc
     1201 ttaccacccc cactctagga tctttttcat aaccaaaacc attaatcgat tgattgattt
     1261 catataaaaa tctcagttca tctctagtta attctttccc cgccttaact ttaccatcaa
     1321 ttaaagttaa cctttccata tccctatctt tcttttcaaa actgcttccg ccaggtagct
     1381 gttgatactt attcttagca atttccagca tattaggttc taaattttga cctggattta
     1441 ttcctctaac ctcaaacacc tccccttctt ccatcctgat agcaatccga ggaattgtcc
     1501 tgttcccttt ctcatcttca gtgtaataca cataaaaatc gcccctactt aattgatcct
     1561 tagccacctc ctcaccagca gtacaccaac cagtcccatg accttgaagc gacttgtaaa
     1621 ggggaatatg gtccccgcct tggtcaaatt taatccactc acccctggtt ttttctttat
     1681 tttcctttga tgctggagta acctcatcaa ttgcaaaagc ataaattcta gagaaacttg
     1741 tcgactctaa gatagacctt aactcctcgc tgacatgacc ttcacgtttt aaataatatt
     1801 tagtaataac atcataacaa taagctaaag cctccctgtt caaatcagga aatatcgtgg
     1861 ttgtgtcctt agttcttttt gaaaactctt ttttgtcttt atcatagaca cccatcttaa
     1921 ttacactctt taagacataa aatttgaacc aggttggata accagcttca ggagatgaca
     1981 aatagtcagc ccaaacatct agtgattttt tttggtcgcg atagatagtt tctccggctt
     2041 tttttctggc ctcaggacta aaatcatctg ctgttctaat cccaaccctg tcataatctg
     2101 ccccttgacc tctttgacga gcaattctca attgtagttc aaaataacta ttaggaacac
     2161 tgtccttatt aatcaaaagc gcttctggat aaagcagttc ttctttaaat ttttttagtc
     2221 gtctctgacg atcagcttca tcaggattat taaatatttt acccatcatt gataaatcag
     2281 gactctcttc tctactgtaa ccatacaaag tctctcctaa aaaagcctct aatcttcctt
     2341 ttttttgaat tctcattgac caaattgcca tccaacttag ccagttcaat cgctttttta
     2401 accaaatccg atttgtgtaa gtcagaaaat aaattgactg gaatttctcc accgctttca
     2461 ctcataaata aatgatacca aaaaaacttt taaacaggtc tataaacttt ataaattata
     2521 ggataaacta ttttttctcc acttttccac atcaataccc tatatttttt tactatctca
     2581 aaacattaat tttataagat attttttagg ttcaaaaaat aacaaatcaa aatctgaagt
     2641 tataattttg aaaaacgaag attttatagt ctagattaag ctataattcc cttatggaca
     2701 ccaacaaact tgtctccgat ctcacagctc aatttcccga tctaaaaatt tattaccagc
     2761 actctttggc tccttatact accgtcaaaa tcggcggtcc ggcggatgtt tttatccact
     2821 ccacctcttc tacccaatta actgaaatct taaaatattt atctagcgta gatagtattt
     2881 tttccgcgcg caaggcgctt aaagactctg atgaaggtgc gagcacggta aaaaatatta
     2941 tcaagccaaa catcactatc ctaggcaatg gttccaacac tcttatttct gactccggcc
     3001 tccgcggcat tgtcatcaaa aacagttccg accaatttac tattttagat tctgtctctc
     3061 ccgctactcc cgcttctacc aaaatttctg cccaccgtac cgaaaacgat cccgccaaat
     3121 atcttaattt ttcccaaatc gactacgatg aatctgatca accccaagtt ttggtcaaaa
     3181 ttgactccgg ttgcaatctg ccatcaacca ttaaccggct gattaatgcc ggcgttacgg
     3241 gtcttcaatg gtttggtttt atccccggca ctatcggcgg cgccgtttat ggcaacatcc
     3301 atggcggcac tcaccatttc tctgatttta tcaaagaggt tgaagttttt gaccttaaca
     3361 ccggtcaaat taaacatatt cctaaaacca atctctcttg ggactacgac cattcctctt
     3421 ttcaatcaca gcccaacttg attattttat ccgtcaccct gtctctttac cgcggcgaca
     3481 ccgcccgagc caaggccgcc gtccaagcct ggattaccca aaaatccgcc gtccaatcca
     3541 tgaattcact tggctctgtt tttcaaaacc ccccactggc tatctgtcaa aaaaattggg
     3601 gcgaacagaa atccgccggc tggattatcg acactcaact caatctcaaa ggcgcccaaa
     3661 tcggcgatgc ccaaatcagc cccaaacatg ccaactttat gataaatcag agtcaagcta
     3721 ctgccaccga ctaccttgcc cttatcaatc tggtccaaac ccaactccgg caaaagttca
     3781 actttcagtt tgatttggaa attaaacttt taggcaaatt cacccactaa atttcaaaac
     3841 taccactttt ttgtttcgtc tttatagact catcagtatt ggccctacca atagacaaaa
     3901 agagtaaaag aagggaccct aaaatagggg taaacctatt aaggttttct tttactccaa
     3961 aaaaaaaggt gagagtttat gccaagcaga ttggccgcgg cgacattgcc actccttcca
     4021 gacactctcc gatccgatct aaattggcat tccggtcatc aaaccaaaaa ccattctgcc
     4081 acagacccaa aaagttgtct gcttgatcta ccagataaat gggaaagctt cttttgtcaa
     4141 aagacaacaa cctggacatc tcaatcaact tcactgcaga gacgtcaatt gttcccacca
     4201 aaaccctggc agaatccaac ccatcggcaa acacaacccc gatagccttc atcaactcca
     4261 aggtcttgtc atcgaccgag acaatgcttc cgtatgtcaa atcttgtgac ggaaacagta
     4321 tgtcgtgccg attaggcttt gtcaccactt gaactctaac tgatgtcatt ttcacaaaat
     4381 atcctcctat cccatttcca ccggcataac cgtatatctt accctatgat cggtattttc
     4441 cattaccaac ccatttcccg actgataata cccttgcacc actcctttct catctaccaa
     4501 aacgattggt aaccctaccg tcaatgctcg tgaccgctcc ttttcattca tcagttcaaa
     4561 aggattgatc gattttgcct tgtctctagt aaagacataa atgcaaaaat taccgtcaac
     4621 ctttacctca gctcccattt ggtgcagaat ctggtccatc ggtcgtcctt ggatcacatt
     4681 atcagactcc aatctgacca tcttcaccat ttccaccatt ctaactgaca tcacatcagg
     4741 caagataaag tatttgaaat aaccatttcc ggtaggaaaa caaagcgtat aacatacccc
     4801 gccgtcacac tctatccaaa tcactccttt cttaaatctc tcccacctct gcgttaagtc
     4861 gtcggtccgt ttcgaaaaca atcccttttc cgtagccagc ataccctcac ccctaaatat
     4921 ttttttaccc gcttttgggt aaaatctaat cacccttaaa tgatcaaatt ccacccaaaa
     4981 cggttatgtt aatgttcctt gtttcgtcct gttggactca tcagtgccag cttcactggc
     5041 agacaaaagt ggggagtaaa aatttataag cctgttaggc catttttact ccccaaaaaa
     5101 aagggttgct aaactatcga caatctgtca tcaaatttgg tcatcctaaa cccaggcaga
     5161 tacagatcaa actccggatt ttccttcgtc cagaacttac cgtcaaccca cagtgccgct
     5221 agatcgcctt tgtcgtcgac aaaataaatt ggccgcaccg ctgccttgta gttggtgatc
     5281 actgagaaat caaccttaga taggcttttt gggtttttga tggtataaac caccattccg
     5341 tccggcaaga tctgtttgtg ggagaccgac tccaaatagt tgacaaacga atcctgaatc
     5401 agcgcgattt catccatctt ttggatatga ctcttattgg ccttgatatc cttctcatac
     5461 tgtgacagtc gccctctcag ctccaccttg gttgtataag ccgacacact caatcccgga
     5521 aaccaaacag tcagataacc tttcctatcc ttcactctga cactaattgc cttcattttt
     5581 tccaaccctt tcttttttaa atgttcacca ctcgcttttg agcagtgtta cattatagct
     5641 caaatcacct cacaaatcaa gattatagga tatactaccc tctcaatacc ctacccacaa
     5701 attttggcag cgctaactgc ctttttattc tctttggctc ttttatcaac gaatatagcc
     5761 attccattcc tatttttcgc cacaccttgg gcgccctttt taaatcccca ctgtaataat
     5821 caaagctccg ccccaccccc atcactactc ccgcatcaat ttttttcaaa ttctcttcaa
     5881 tccacatttc ctgccgtttc atcccatatg ccaccaacag cacgtcaggt ttaaatttat
     5941 taatatcctt ttttacttcc tcatttgtta ctgttggctc tccggcgcaa gctcttactt
     6001 g
//
LOCUS       DAOSHV010000028         5977 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_11710_length_5977_cov_5.314083, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000028 DAOSHV010000000
VERSION     DAOSHV010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 5977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5977
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11710_length_5977_cov_5.314083"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..75
                     /locus_tag="PKZ29_01610"
     tRNA            1..75
                     /locus_tag="PKZ29_01610"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:34..36,aa:Ala,seq:cgc)
     gene            86..1618
                     /locus_tag="PKZ29_01615"
     CDS             86..1618
                     /locus_tag="PKZ29_01615"
                     /inference="COORDINATES: protein motif:HMM:NF025433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LssY C-terminal domain-containing protein"
                     /protein_id="HOG37601.1"
                     /translation="MVKIIKSVTKSIGRGVKNDPEVSKIIQRYPKLFRFLKKRLTPTE
                     KYGLHFTIGFLITLFFIYIFFGILQDYIGNENIIQFDLRVLNLFSALRHPKLNQQMLF
                     ITYLAKGEIIIIGTIITSLILFLFKKRRFIKTLLISVLGGEVLVWLIKHLISRPRPPL
                     TDSLVIEPSYSFPSGHTFVALAFYGLLAYFVIQSERRKFLKVIIFLISLSLISLVGIS
                     RIYLGAHWPSDVLASLAVGSAWLVIMATSLKIKKKFTPPKPDSIQFHPVVARSLSVLL
                     ILTWIAFIYSYYYTHPLAKSPQNQPPPITTVTPDQIKHQLFQQLPKVSESINSYPAEP
                     INIIIVGSESLINRAFTDIGWYLLDRPGIKVYAKLVNSVIFNQPYPNAPGLPVFWDTK
                     PNLIGFGRPDTPGSVSSRHHIHFWKTNFVTPDNQPVWVGTAHFDQNIKKKFKIVMPFH
                     QTELLVDNEREDIKNQLTHNGFVKSFEKINLTGLTYGTKKSGNNFLTDGQAYILYLQL
                     PK"
     gene            1615..1914
                     /locus_tag="PKZ29_01620"
     CDS             1615..1914
                     /locus_tag="PKZ29_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37602.1"
                     /translation="MRQDKPTPVSQQLEKFKDTLKQRQKYVKMEYQAYGLELAEELGD
                     WKNRSLYIRLAKNTNRNVLEQARYFVKDQSPGTIKTPYKLFMWKLRQLKADKTQT"
     gene            1931..2983
                     /locus_tag="PKZ29_01625"
     CDS             1931..2983
                     /locus_tag="PKZ29_01625"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOG37603.1"
                     /translation="MKFLKKYRKKIILGIAIALILFLGFRQIQAQKYKNPQVFNPKKD
                     TVVTPKTKDINYSITLSGSIDAKDKAEVRFQTPGRLIWVGVKEGDRVKKWQSLASLDR
                     QELRKQLEISLNNYKTQLSQFDDTQDKYKTEKDNLTLTDEMKRILNRSQYSLNNSVIS
                     YELNDLAIKYATIWSPINGIVTGAEPANPGVNITPASSAFTIIDPNSVYFKSEIDEDD
                     VTRVKVGQSASLKIDAYPQETFDSEITNIAFTPVPGQTSTVYKIDFKLHVDNQDMRYR
                     LGMDGDATIILDEETNALVLPLEAINYEDDKTYVWVKKDQTLEKIYIDTGIETDTEVQ
                     VLSGISINDSIIVKKI"
     gene            2955..3662
                     /locus_tag="PKZ29_01630"
     CDS             2955..3662
                     /locus_tag="PKZ29_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009843640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG37604.1"
                     /translation="MTQSSSRKSKSPIVLELKNIVKTYITGGDSFNALDGVSLSIRRG
                     EFISITGPSGSGKSTLMHIIGLLDNPTNGKVLLEGRDISQLKENDLARIRNQTLGFVF
                     QQFNLLAKTSALENVALPLLYSDTPASDRELISRQMLDKVGLSDKLKNTPAQLSGGQQ
                     QRVAIARALVNHPKIILADEPTGNLDSKSGQEVISLIHQLHREGNTIVLVTHDPNLAN
                     QAQKVIIIKDGKIKPHD"
     gene            3655..4872
                     /locus_tag="PKZ29_01635"
     CDS             3655..4872
                     /locus_tag="PKZ29_01635"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG37605.1"
                     /translation="MIKPSLKDLLSSSIRSVLKNKSRTILTSLGVIIGVTSVILLSSI
                     GNGLKSFVAEQFETLGANSIFISPGKIFNETGGFNNSGGMSTTTFTQKDLNILKKEIK
                     NTTIIPINFIFIDIKSANATKKQAELDGSIEAMGQASNLLPSPGNGRWYTDEENAKKS
                     PVIVLGYKVAETLFPNSKAVGKNVIIKGKTLKVIGVVDQKGSSMGGPNIDEQAYAPFN
                     LVADLSGNDNIISFMAKAKDRNSVDQVKKNITKTMEKRYDKDSFSVYDSSQLLKSINS
                     IISVLTTALTGIAAISLVVGGIGIMNIMLVTVSERTREIGLRKAIGAYPRAILIQFLF
                     EAVILSGLGGIIGIILGSLGTAAINNFFPAKVTFNSILTAFGVSFMVGIIFGVTPARK
                     ASQLSPIEALRYE"
     gene            4895..5458
                     /locus_tag="PKZ29_01640"
     CDS             4895..5458
                     /locus_tag="PKZ29_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37606.1"
                     /translation="MKYKFRHHLLASTILFLFIAIFLVVFRVNNLQLLFLFLGIILGM
                     FLLDLDHFIFWYFLKPNLEESRLARTALSRHDFKSIFRLIKISHNTHHNLIFHHYFFQ
                     IILVLFSFFIFTSTNNLFVHSLTISLNLHLLIDEYIDYFRDPKILQKWLFTREQTQLP
                     VKFLGQYLAVFTVFFVIFVTLLIKFQI"
     gene            5455..5955
                     /locus_tag="PKZ29_01645"
     CDS             5455..5955
                     /locus_tag="PKZ29_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37607.1"
                     /translation="MMVSRPELFSEAKKYLSGHQSNLVNDVEIILGRLKINDDISDFS
                     FLVSPIAKSYEGYLKDFFLKIGIISNQEYESDRFRVGKTLNPSLRYKRFSVYQKLSDY
                     DDRGEELAEILWDAWKFGRNEIFHYFPNNYKNLSRNEAEDRINLLLRAIIQAGNFLDN
                     NQKQQF"
BASE COUNT         1750 a         1424 c          987 g         1816 t
ORIGIN      
        1 gggcctttag cttatctggt agagcacggc attcgcattg ccgagagggc ggttcaagtc
       61 cgccaaggtc caccaaccaa aatctatggt caaaattata aaatcagtta ccaaatccat
      121 cggccgcggc gtcaaaaacg atcctgaggt cagcaaaatt attcaaaggt atcctaagtt
      181 attccgtttt ctcaaaaaac gtctcactcc gaccgaaaaa tacggcttac atttcactat
      241 cgggttttta attaccttat tctttattta catttttttt ggcattcttc aggattacat
      301 cggcaacgaa aatatcatcc agtttgacct cagagttctc aatctcttta gtgctctccg
      361 ccatcccaaa ctaaatcaac agatgctttt tatcacttat ctggccaagg gcgaaatcat
      421 tattattggc accattatca cttctttaat tttatttttg ttcaaaaaac gccgtttcat
      481 caaaacttta ttgatctctg ttctcggtgg ggaagtcttg gtttggctga ttaaacatct
      541 catcagtcga ccgcggccac ctctaaccga ttcacttgta atcgaaccaa gttacagttt
      601 ccccagcggc catacctttg ttgctctggc tttttatggc ctcctagcct attttgtcat
      661 ccagtctgaa agacgtaaat ttttaaaagt gattatcttt ttgattagcc tttctcttat
      721 cagcttagtt ggtatctccc ggatttattt gggagctcat tggccctccg atgtccttgc
      781 tagtctggct gtcggcagcg cctggttagt cattatggct acttccctca aaatcaaaaa
      841 aaagtttact ccacccaaac cagattccat tcaatttcac cccgttgttg ccagatcctt
      901 gtccgttctc cttattctca cttggatagc ttttatttac tcctattact acactcatcc
      961 tttggcaaaa tcaccccaaa accagccacc ccccattacc accgttactc ccgaccaaat
     1021 caaacaccaa ctttttcaac agctccccaa ggttagtgaa tctatcaact cctatcctgc
     1081 cgaaccaatt aacattatta ttgtcgggag tgaatctctc atcaaccgcg cctttactga
     1141 cattggctgg tatcttttgg accgacccgg aatcaaagtt tatgccaagt tggtaaattc
     1201 cgttattttt aatcaaccat atcccaacgc tcccggactc cctgtttttt gggataccaa
     1261 acccaaccta atcggctttg gcaggccaga tactcccgga tctgtcagct cccgtcatca
     1321 catccacttt tggaaaacca attttgttac tcctgataat caaccagttt gggtaggaac
     1381 tgcccatttt gaccaaaata tcaaaaaaaa gtttaaaatt gttatgcctt tccaccaaac
     1441 cgaacttttg gttgataatg aaagagagga tataaaaaat cagctgactc acaacggctt
     1501 tgttaagtca tttgaaaaaa taaatcttac cggactaacc tatggcacca aaaaaagcgg
     1561 caacaacttc cttaccgacg gccaagccta cattttatat cttcagttgc ccaaatgaga
     1621 caagacaaac ccactcccgt ctctcaacaa ttggaaaaat tcaaagatac tcttaaacag
     1681 cgccaaaaat acgtcaaaat ggaatatcag gcctacggtc tggaattagc cgaagaatta
     1741 ggcgattgga aaaaccgctc actttatatc cgtctggcca aaaataccaa ccgaaatgtt
     1801 ctggaacaag cccgatattt tgtcaaagat caaagtccgg gaactatcaa aactccatat
     1861 aaactgttta tgtggaaatt acgccaactt aaagccgaca aaacccaaac ttaatgatac
     1921 aattgcttgc atgaagtttc tcaaaaagta tcgtaaaaaa attattttag gtatcgccat
     1981 agctttgatt ctttttctgg gttttcgcca aatccaagct caaaaataca aaaaccctca
     2041 agtttttaat cctaaaaaag atactgttgt tacaccaaag acaaaagata ttaattacag
     2101 tattacttta tctggttcga ttgatgccaa agacaaagct gaagtccgat ttcaaactcc
     2161 aggcagacta atttgggttg gtgtcaagga aggggatagg gtcaaaaaat ggcagtccct
     2221 ggcctctctt gacagacagg aactccgaaa acaactggaa atctctctta acaattacaa
     2281 aacccaatta tctcaatttg atgataccca ggataaatac aaaaccgaaa aagataattt
     2341 aaccttaacc gacgaaatga agcgcatcct caaccggtct caatattcgc tcaataactc
     2401 tgtcatcagc tacgaactaa atgatttagc catcaaatat gccactattt ggagtcccat
     2461 taacggcatt gtcactggcg ccgagcctgc taaccctgga gtcaacatta ctcccgcttc
     2521 ttccgctttc accatcatcg accccaattc agtttatttt aaatccgaaa ttgatgaaga
     2581 cgatgtcacc cgagtcaaag ttggtcaatc agccagtcta aagattgatg cctatcctca
     2641 ggaaacattt gattcagaaa ttaccaatat tgcctttacc cctgtcccgg ggcaaacctc
     2701 cacagtttac aagattgatt ttaaactaca cgttgataat caggatatgc gctatcgtct
     2761 gggtatggat ggtgatgcca ctattatcct tgatgaagaa accaacgctt tagttttgcc
     2821 gcttgaggct atcaattatg aagatgacaa aacctatgtt tgggtaaaaa aagatcaaac
     2881 cttagaaaaa atttacatag ataccggaat tgaaactgat actgaagttc aggtcttgtc
     2941 cggtataagc atcaatgact caatcatcgt caagaaaatc taaatcaccg attgttttgg
     3001 agctgaaaaa tatcgtcaaa acctatatta ccggcgggga tagtttcaat gccctcgatg
     3061 gtgtatctct ttcaatccgc cggggtgaat ttatctctat caccggtccc agcggttctg
     3121 gcaaatctac ccttatgcac atcattggcc tccttgataa tcctaccaat ggcaaagttt
     3181 tgctcgaagg tagagacatc tcccagctaa aagaaaacga cctagctaga atcagaaatc
     3241 aaactctcgg ttttgttttt caacaattta atctcttggc caaaacttca gctctggaaa
     3301 atgtcgccct accactcctt tattctgaca ctcccgcctc agaccgcgaa ctaatctccc
     3361 gacagatgtt ggacaaagtt ggtctcagtg acaaactcaa aaatactccc gctcaactct
     3421 ccggtggtca gcagcagcgg gtagctatcg cccgagccct ggtcaaccac cccaaaatta
     3481 ttcttgccga cgagcctacc ggcaatcttg attccaaatc cggtcaagag gtcatttctc
     3541 ttattcacca gcttcatcgc gaaggtaaca ctattgtttt ggtcactcat gaccctaatc
     3601 ttgctaatca agcccaaaag gtcattatta ttaaagatgg caaaataaaa cctcatgatt
     3661 aaaccatctc tcaaagatct gctcagcagc agtatccgat cagttctaaa aaataaaagc
     3721 cggactatcc ttacctcgct gggtgttatt attggtgtaa cttcagttat tctgcttagt
     3781 tctatcggca atggtctaaa aagttttgtc gctgaacaat ttgaaactct tggtgctaat
     3841 tctatcttta tctctcccgg taaaatcttt aatgaaaccg gcggttttaa taatagcggc
     3901 ggcatgtcaa ccactacttt tactcaaaaa gacctcaata ttctcaaaaa agaaatcaaa
     3961 aataccacca ttattcccat aaactttatc tttattgata taaaatctgc caacgccacc
     4021 aaaaaacagg ccgaattaga tggttctatc gaagctatgg gtcaagccag caacctactt
     4081 ccttcacccg gcaacggtcg ttggtatacc gatgaagaaa atgccaaaaa gagccccgtc
     4141 attgtccttg gatacaaggt tgctgaaacc ttatttccca attccaaagc tgtcggcaaa
     4201 aacgtaatta ttaaaggcaa aactctaaag gttattggtg ttgttgacca aaaaggatcg
     4261 tctatgggcg gtcccaacat tgatgagcaa gcttatgctc cctttaatct agtcgcagac
     4321 ctatccggca acgacaatat tatttctttc atggccaaag ccaaagaccg taattcagtt
     4381 gatcaagtca aaaaaaatat taccaagacc atggaaaaaa gatacgacaa ggattctttt
     4441 tcagtttacg acagttccca acttttaaaa tcgatcaatt ctattatcag cgttcttacc
     4501 actgccctaa ccggcattgc cgccatctct ctggttgttg gcggtatcgg cattatgaat
     4561 atcatgttgg ttactgtttc tgagcggacc agagaaattg gtctgcgaaa agccatcggc
     4621 gcctaccctc gcgctattct cattcaattt ctttttgaag ccgttattct ctccggcttg
     4681 ggtggaatta ttggtattat attaggttcc ttgggcacag ccgctatcaa caatttcttt
     4741 cctgccaaag tcactttcaa ctctatttta accgcttttg gggtttcttt tatggttgga
     4801 attatctttg gtgtcactcc cgcccgcaaa gccagtcagc tttctcccat cgaagccctc
     4861 agatacgagt aaaactttct gctatcattt attagtgaaa tataaattcc gccaccacct
     4921 gttggcctct accattcttt ttctttttat cgctattttt ttggtggtct ttagggttaa
     4981 caatcttcag ctactatttc tctttttggg cataattttg ggcatgtttt tacttgactt
     5041 agatcacttc attttttggt attttcttaa accgaatttg gaagaaagcc gcttagcccg
     5101 caccgctctc agtcgccatg actttaaatc tatttttcgg cttatcaaaa tcagtcacaa
     5161 cactcatcac aaccttattt ttcaccatta ttttttccaa attatcctgg ttcttttttc
     5221 cttctttatt tttacctcca ccaacaatct ttttgtccat agtctcacca tttctcttaa
     5281 tctccacttg ctgattgatg aatatattga ttattttcgc gatcccaaga tactccaaaa
     5341 atggctcttt acccgggagc aaacccaact tccggtcaaa tttttgggtc aatatttggc
     5401 cgtctttacc gttttttttg ttatatttgt tactttacta attaaatttc aaatatgatg
     5461 gtctcccgcc ccgaactttt ttctgaagcc aaaaaatatc tttccggtca tcaatctaac
     5521 ctggtaaatg atgttgaaat aattcttggt cggctcaaaa tcaacgatga tattagtgac
     5581 ttttctttcc tggtttctcc catagccaaa tcatacgaag gttatcttaa ggattttttc
     5641 cttaaaatag gtattatctc caatcaggaa tatgaatctg atcgtttccg tgtcggcaaa
     5701 actctcaatc cctcacttcg gtacaaacga ttttctgttt atcaaaaatt atccgattat
     5761 gatgatagag gagaggagct ggccgaaatt ctctgggatg cctggaaatt tggccgcaac
     5821 gaaatattcc attattttcc caacaattat aaaaatttat cccgaaacga agctgaagac
     5881 agaatcaatc tcttactccg cgctattatt caggcaggga attttttgga caataaccaa
     5941 aaacagcaat tttaactcaa acccttgtgg cagatat
//
LOCUS       DAOSHV010000029         5805 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_12182_length_5805_cov_6.112174, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000029 DAOSHV010000000
VERSION     DAOSHV010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 5805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5805
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12182_length_5805_cov_6.112174"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            100..621
                     /locus_tag="PKZ29_01650"
     CDS             100..621
                     /locus_tag="PKZ29_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37608.1"
                     /translation="MNKKILWLGLVILVIIGASVYLRGRGKIIPNNEDNTPKKIEVKK
                     MPYNGSKKACDVMSPEIAEALFSPVVKPVENNGDDRVCVYADKEKLGISHYVLMMSVG
                     TARDEDYKRYEDKNYKKVEGLGEVAYWDESRSVLDIRAGDTSYKIGYQANGPSTLQTV
                     RPVAELIKERFLK"
     gene            complement(599..1117)
                     /gene="pth"
                     /locus_tag="PKZ29_01655"
     CDS             complement(599..1117)
                     /gene="pth"
                     /locus_tag="PKZ29_01655"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOG37609.1"
                     /translation="MKLIFGLGNPDKKYQATRHNLGQKFIRQYLDQCYLSLAPKKTLL
                     AEICEVGQGSQKNIFAISTGYINNSGQTIQKLVNFYKLDPQDILVVHDDLDLPVGEYR
                     IQFDRGPAGHNGIKSIIEHLHTQAFHRLRIGIDKPNSPIPTEDYVLQPFSPSEQEIIK
                     QTLDKITSKIFP"
     gene            complement(1228..3204)
                     /gene="ligA"
                     /locus_tag="PKZ29_01660"
     CDS             complement(1228..3204)
                     /gene="ligA"
                     /locus_tag="PKZ29_01660"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOG37610.1"
                     /translation="MITLDSAELKNISPESLGELLIEAKKAYYTGGKPIMDDHTYDVL
                     ENTLHRQNPYHRIFSKVGTDDFNTGFDKKSHFLPMGSQNKVNTYADLVHYFQLKKIPQ
                     NSKFLVQVKCDGISLEIEYNQGQLVDAITRGNGLIGDIITQNVVKMKNFVPRLPQKFS
                     GSIRCEILVTKKDFIKLNKIVEKQVLPPLSKGGDIVTTISEGFYSNPRNAASGLSQRL
                     DGLNSHLCSLYATDISGTFATESEKIDLLKTLGFTPVESHLCHTFEAIEKIYQNFLHR
                     DRESYPYDIDGLVIKINDLKIAKKLGQKNGRPKYQVAYKFPADSNQSQIKNITWQVGP
                     LGSVTPVAEIEPIEISGAVINFASLGNYDLVAKKNLNLGDIIEISRRGDVIPHIEKVI
                     TKVNPGTASIPTHCPACKTVLIKENKSLRCPNSLKCLPQILGSLKLFCDKLQILGLSD
                     KTIKKLYHSDKLKLPGDFYRLTLNDIQDLENLGEKSAKNILRQIDLKKHLTLKEIFDA
                     ASIPNFSAARIQQLIIAGFDTPKKLLNLNLADFLAIKGFKTTLAQKILNGLNQRRDWI
                     ESILNQVTLKNNSAPKTKLSGLSFAITGTLSLPRKQLEEKIISLGGKVSNTVTSHTNY
                     LITNEISSTSDKFISAKKIGTKIITESDFHQLAD"
     gene            complement(3237..4364)
                     /locus_tag="PKZ29_01665"
     CDS             complement(3237..4364)
                     /locus_tag="PKZ29_01665"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOG37611.1"
                     /translation="MFSPVFSINNQILKNIGQIEAAKAIIDEAPMIPAYEKQFQQEAI
                     VRTVHYGTRIEGNDLTFQEVARLVEGHQITATDRDIQEVINYRNVMSYLEELWSLYDA
                     NMPLPEEKNIDTNDKKRPFFYSETIIKRIHQLTTAKIIPESDVGKYRHQQVVLKNTRT
                     GEIAHRPPPAIEVPYLMTDFIDWINSSASQEIHATIKSAISQYILVAIHPFIEGNGRV
                     SRAFAMLPLYTQGYDIRRLFSMEEYFDRNAEDYYNTIQNTHLLSSDIFKRDLTKWIEY
                     YTQALAVELSRVKDKVQSLSRDIKLKQKLGGKQVHLSERQIKLVEYMQQFGGLRMPDA
                     QQLLPMVSDDTIWRDLKKLVDSGAIEKRGSTKGAYYCLTNV"
     gene            4466..5413
                     /locus_tag="PKZ29_01670"
     CDS             4466..5413
                     /locus_tag="PKZ29_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37612.1"
                     /translation="METSYSPERTLDIKVFKADAQNYDFLSRHLTAEPDKWKDMTEVA
                     SEYIKCTKDVIKVLDGTDNRRDIKDDDKEAVAAGAPDVVIYLDKSARPVSWLVDKFWS
                     ETADKNAVKPMTKFLSIDRDDKTWEDLEMTGAREPTDKIYNFDRLSKEKVLAVRAIFH
                     RGELLPNNWKQEVVKNNDLDQKNILIVDEVKSSGRTLMIAQKLLKRAFPTSNISGVYF
                     WDSRFKLIRSSGQTVQQMTSVPVWYNQREIGGRGVGNKDESYYARTPHEFKVRLGSFL
                     LSAPQFGEGYRRLKVSDDKLSVALRKDINQLHQDYVRSR"
     gene            complement(5416..>5805)
                     /locus_tag="PKZ29_01675"
     CDS             complement(5416..>5805)
                     /locus_tag="PKZ29_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012157947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37613.1"
                     /translation="QNALLKTIEDPGPNNYIIITTAKPSKILPTIISRCHSIKLKNLK
                     QTSPNLTPLVISHQIKTDLALSDSLSKDKNSVLPYLQQQLSFYQQTLIKQPTLKNSRL
                     IQKLLTAISMIESNVDPKSALDYFFLN"
BASE COUNT         1681 a          831 c         1533 g         1760 t
ORIGIN      
        1 gaaccgccct ctcggcaatg cgaatgccgt gctctaccag ataagctaaa ggccctaatt
       61 gtctagacaa ttattatatc ttaatctaga atcaagcata tgaataagaa aatattgtgg
      121 ttgggattgg ttattttagt gataatcggc gcatcagtat atttgagagg aagaggaaag
      181 ataatcccaa ataatgagga taatacgcca aagaaaatag aagttaaaaa aatgccgtat
      241 aacgggagta aaaaggcttg tgatgttatg tcgccggaaa ttgcagaagc tttgttttcg
      301 ccggtagtta aaccagtgga aaataatggg gacgatcggg tttgtgtcta tgctgataaa
      361 gaaaagttgg gtatttccca ttatgtattg atgatgtcgg tagggacggc aagggatgaa
      421 gattataaaa ggtatgaaga taagaattac aaaaaagttg aagggttggg tgaggtggca
      481 tattgggatg aatctagaag cgtgttggat attagagccg gtgatactag ttataaaatt
      541 ggttatcagg ctaacggtcc gtcaacccta cagacagttc gaccggtggc agaattaatt
      601 aaggaaagat ttttgaagta attttatcaa gagtttgttt gattatttct tgttcggagg
      661 gagaaaatgg ttggaggaca tagtcttcgg tggggatggg agaatttggt ttgtcaatgc
      721 cgattctgag acggtgaaag gcttgagtgt ggagatgttc aatgatagat ttgatgccgt
      781 tatggccggc aggaccccgg tcaaattgaa tccgatactc ccctaccggt aaatcaagat
      841 cgtcgtgaac aactaagata tcttggggat caagtttata aaaattaact aatttttgga
      901 tggtttgacc agaattgttt atatagccgg tagaaatggc aaaaatattt ttttgactcc
      961 cctgcccgac ttcacaaatt tctgccagta aggttttttt gggagcgaga gagagataac
     1021 actgatcaag atattgtctg atgaattttt gtcctaaatt gtggcgggtg gcttgatatt
     1081 ttttatctgg attgcccagg ccaaagatta atttcataga atagtttagc atttggaatt
     1141 ttgatagtta aatatatatt ttgatatata tttatagcct aagaatagga aagaatgttt
     1201 tttatatatt tgttagcagt ctagttgcta gtctgctaat tggtggaagt ctgattcggt
     1261 aataattttg gtgccgattt ttttggcaga aataaatttg tcggaagtag aactgatttc
     1321 gttggtaata agataattag tatgggaagt aacggtattt gagactttgc caccaaggga
     1381 gataattttt tcttcgagtt gtttgcgggg aagcgataag gtgccggtga tagcaaaact
     1441 aagaccggat aatttagttt ttggggcaga gttatttttt agagtgacct gatttaggat
     1501 agactctatc cagtcacgcc gttggttgag tccgttgagg attttttggg ctaaggtggt
     1561 tttgaaacct ttgatggcca aaaagtcagc aagatttagg tttaaaagtt tttttggggt
     1621 atcaaaaccg gcaataataa gctgttggat gcgggcggcg gaaaaattgg gaatggaagc
     1681 cgcatcaaaa atctccttga gagtaagatg ttttttgagg tcgatttggc gaaggatatt
     1741 tttggctgat ttttcaccta aattttccag atcttggata tcgttgagag tgagtcggta
     1801 aaaatctcca ggaagtttga gtttgtctga gtgatagagt tttttgatag ttttgtcgga
     1861 gagaccgagg atttggagtt tgtcgcaaaa tagtttaaga gagcctaaaa tttggggcag
     1921 gcactttaga gaattggggc agcggagaga tttgttttct ttgataagaa cggttttgca
     1981 ggcgggacag tgagtgggaa tagaggcagt tccaggatta actttggtga taactttttc
     2041 gatatgggga ataacatcac cgcggcggga gatttcaatg atgtcaccga gatttaaatt
     2101 ctttttggca actaaatcat aattacccag agaggcaaaa ttaatgacgg caccagaaat
     2161 ttcgatgggt tcaatttcgg ctacgggagt gacagatccc aaaggtccga cctgccaggt
     2221 gatatttttg atttggcttt ggttggaatc ggcgggaaat ttgtaggcga cctgatattt
     2281 gggtcggcca tttttttgac ccaatttttt ggcaatttta agatcgttga ttttgataac
     2341 caaaccgtcg atgtcgtaag ggtatgattc tcggtcgcgg tgtaaaaagt tttggtagat
     2401 tttttctata gcttcaaagg tgtggcaaag atgggattcg actggggtaa aaccaagagt
     2461 ttttagaagg tctatttttt cggattcggt ggcaaaagtt ccagaaatat cggtggcgta
     2521 aagactacaa agatgggaat tgagaccgtc aagtcgctga gaaagtccgg aagcggcgtt
     2581 acggggatta gagtaaaatc cctccgatat cgtagtaacg atatcacctc cctttgacaa
     2641 gggaggtaga acttgtttct cgacgatttt atttaatttt ataaagtctt ttttggtgac
     2701 gagaatttcg cagcggatgg agccggaaaa tttttggggc aatcggggga caaaattctt
     2761 cattttgacg acattttggg tgatgatatc ccctatcaga ccgttgccgc gggtaatggc
     2821 gtcaaccagt tgtccttggt tgtattcaat ctccaaagaa atgccgtcgc atttgacttg
     2881 gactaaaaat ttactattct gaggtatttt tttgagctga aaataatgga ctaaatcggc
     2941 ataggtgttg actttgtttt gggaacccat ggggaggaag tgagattttt tgtcaaagcc
     3001 ggtattgaaa tcatcggtgc cgactttaga aaaaatccga tggtaagggt tttgccggtg
     3061 aagagtgttt tcaagaacat cgtaggtgtg atcgtccata ataggtttac cgccggtata
     3121 ataagctttt ttggcttcaa tcagaagttc gcccaggctt tcgggagaga tgtttttgag
     3181 ttcggcagaa tcaagggtaa tcataagtta ttataacggc cctagagaaa aaatcgttag
     3241 acattggtca gacaataata ggcgcccttg gtggaaccgc gtttttcgat agcaccagag
     3301 tcgactagtt ttttgagatc gcgccaaata gtatcatcgg aaaccatagg cagaagttgt
     3361 tgagcatcgg gcatgcgtaa accgccaaat tgctgcatat attcaactaa tttaatttga
     3421 cgttcgctta ggtggacttg tttgccgcca agtttttgtt tgagtttgat gtcgcgggaa
     3481 agagattgga ctttgtcttt gacccgggag agttcaacgg ccagagcctg ggtataatat
     3541 tcaatccatt tggtcagatc acgtttaaaa atatcggagg agagtaaatg ggtgttttgg
     3601 atggtgttgt aataatcttc ggcattgcgg tcaaaatatt cttccataga aaaaagacgg
     3661 cggatgtcat aaccttgagt ataaagaggt agcatggcaa aagcacggga aacacggcca
     3721 ttaccttcga taaaagggtg aatggcaact aaaatatatt gactgatagc tgatttgatg
     3781 gtggcgtgga tttcctgaga agcggagctg ttaatccagt cgataaaatc ggtcataaga
     3841 tacggaactt cgatggctgg cggaggtcgg tgggcaattt cgccggtgcg ggtgtttttg
     3901 agaacgactt gttggtgacg gtatttacca acgtctgatt cgggaataat tttggcggtg
     3961 gtcagttggt gaatccgttt gataatagtt tcactgtaga agaaggggcg ttttttgtcg
     4021 ttagtgtcga tatttttctc ttccggcagg ggcatattag catcgtaaag agaccaaagt
     4081 tcttcaagat aagacataac gttgcggtag ttaataactt cctgaatatc tctgtcggta
     4141 gcagtaattt gatgaccttc gaccaagcgg gcaacttcct gaaaggtaag gtcgttaccc
     4201 tcgatgcggg tgccatagtg gacggtgcgg acaatagctt cctgttgaaa ttgcttttcg
     4261 taggcaggaa tcatcggagc ctcatcgatg atagctttgg cggcttcgat ttggccaata
     4321 ttttttagaa tttgattatt gattgagaag acaggagaaa acattgtaag ctgattatag
     4381 catttttggg acaataatta aggggaaagt gcggaaatca gatgctttag tttgactttg
     4441 gcgggagtga ctgttagtaa tatttatgga aacatcatac tctcccgaaa ggacgttgga
     4501 tataaaagta tttaaagctg atgctcaaaa ttatgatttt ttgagccgtc atttgacggc
     4561 ggaaccggat aagtggaaag atatgactga ggtggcgtcg gagtatataa aatgtactaa
     4621 agatgtaatt aaggttttag atgggacaga caataggcgg gatattaagg atgacgacaa
     4681 ggaagcggta gcggcagggg ctcccgatgt ggtgatttat ttggataaat ctgccagacc
     4741 tgtgtcgtgg ttggtggata aattttggtc ggaaacggcg gataaaaatg cggtcaagcc
     4801 gatgactaaa ttcttgagca tagaccgtga tgataaaact tgggaggatt tggagatgac
     4861 tggagccaga gagccaactg ataaaattta taattttgac agattatcaa aagaaaaagt
     4921 attggcagtt agggctattt ttcatcgggg ggaattgttg ccaaataatt ggaaacagga
     4981 agtggtaaaa aataatgatt tggatcaaaa aaatatttta atagttgatg aggttaagtc
     5041 cagtggtaga acattaatga ttgcccagaa gttgctaaaa cgagcttttc caacttcaaa
     5101 tatcagtggc gtctattttt gggactcaag atttaaatta atccggagta gcggacaaac
     5161 ggtccagcag atgacaagtg tgccggtatg gtataaccaa cgagaaattg ggggtagagg
     5221 tgtaggtaac aaggacgaat cttattatgc caggacgcct catgagttta aggttagatt
     5281 aggatcattt ttgttaagcg cgccgcagtt tggcgaggga tataggcgat tgaaggtcag
     5341 cgatgataaa ttatcagtgg cgttaagaaa ggatatcaac caattgcatc aggattacgt
     5401 caggagtcga taaaatcagt ttaagaaaaa ataatccaaa gctgacttgg ggtcgacatt
     5461 actttcgatc atgctgatag cagtcagaag tttttgaatc aggcgggaat tttttaaagt
     5521 tggctgttta ataagggttt gttgataaaa gcttagttgt tgctgtaaat agggcaaaac
     5581 gctatttttg tctttggata aagaatcgct tagggcaaga tctgttttga tttgatgact
     5641 aataactaaa ggggttagat tggggctggt ttgtttaagg tttttaagct tgatagaatg
     5701 acaacgggag ataatggtgg gcaaaatttt tgagggtttg gcggtagtaa taataatata
     5761 attgttggga ccggggtctt cgatggtttt gaggagagcg ttttg
//
LOCUS       DAOSHV010000030         5801 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_12191_length_5801_cov_8.514271, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000030 DAOSHV010000000
VERSION     DAOSHV010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 5801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5801
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12191_length_5801_cov_8.514271"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /locus_tag="PKZ29_01680"
     CDS             complement(<1..818)
                     /locus_tag="PKZ29_01680"
                     /inference="COORDINATES: protein motif:HMM:NF019138.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOG37614.1"
                     /translation="MPRKKISGGVAEPKIDKTVTEISEVKKVEPEKAPIIETKSNNTV
                     SFVKPQTAMPGAAAVQKPTPVRMPVRPAVNYAQTVVTEPIVGVLEIMQDGSGFVRPHF
                     LPNGKDAFIASMQIRRYGLRAGDMIAGAAKKPKEGEKYWAMVRIDKVNEGDLASALRR
                     VEFKDLVPVYPNQQMKLETESEILSTRILDLFCPVGFGQRGMVVSPPKAGKTTLLKEI
                     AAGVAKNYPQAHLMAVLVGERPEEVTDMQRFIKGDVVASSFDQKPEDQTRAAEIA"
     gene            complement(1809..2258)
                     /locus_tag="PKZ29_01685"
     CDS             complement(1809..2258)
                     /locus_tag="PKZ29_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37615.1"
                     /translation="MYIEKGGLGFNCGRAVTFLAQKRVKKMRQGKGRKKKMSMATSLA
                     SATIVGALKDHFKNFTIEKITMAGAWGIDRDAQEGGFWNFPNKPLTLVSFRVFDPREP
                     KSSVKRHYFLMLKEDLHKPEVLQEVEDILTEQEKEREVKEKKEKDTD"
     gene            complement(2346..3680)
                     /gene="radA"
                     /locus_tag="PKZ29_01690"
     CDS             complement(2346..3680)
                     /gene="radA"
                     /locus_tag="PKZ29_01690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HOG37616.1"
                     /translation="MKNKESMFVCSNCGNEYAKWQGQCSACGQWNSLKEIKGTKIQNS
                     RFKKAMKVEVKNLSEINQNSDKKQKSPLIFSTGIKEFDRVLGKGIVQGSAVLFAGEPG
                     IGKSTLLTQLVGKVGGLYVAGEESAEQIGLRVDRLGLNAEKFDILETNRVEEIEEYLD
                     RVNNVYKIAVVDSIQTMVSESQSTISSSRAGVGSVGQIRETTFRLVQLAKQKNITIFI
                     VGHVTKEGDIAGPKILEHMVDTVLYFEGEKNGDLRMLRTTKNRFGATDEVGVFKMEER
                     GLREIGSEEINLISGENNLAGKAVTVTLEGSRPMLVEIQALVVESFAPMPKRVFSGID
                     YNRGQLLVAIGQKSLGMPLFKCDVFVAVSGGIRIEDTGADLAVLAAMWSSYKSAPLSV
                     GDSSGGKKMPVYVGEVSLLGEVKKVRGWEKRKKEAELLGRKMVEIKNVRELK"
     gene            complement(3792..4922)
                     /locus_tag="PKZ29_01695"
     CDS             complement(3792..4922)
                     /locus_tag="PKZ29_01695"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOG37617.1"
                     /translation="MTKKEKKAKKTKTAKKNEILTMADLLKMEGIQELGFKKGQEVKG
                     KIAVIKNKAIYIDIGGKTDAVVMGKEFEFVKDYINDLKVGDEIEVQVKQPENDKGQIL
                     VSIRGAASGYGWNYFSEKEKSGGEVTVFAKELNRGGAVVIAPFGFFGFIPGSQIGSKY
                     DGDPDKMIGKKIKTKVLEVDQAKNRLVFSERLVSEPDKVGEEVGAIENLKLDEVFEAE
                     VMRVEPFGIFVRVNCDNEGKVLNLEGLVHISEVSWEKVSELSTMFKVKDKLKIKLINK
                     DDGRLQFSIKRLTNDPWDKIEDKYPKDKETEGEVVRIANFGALVKLETGVEGLIHVSK
                     LTGGTNLKEGDKVQIYIESIDVQKRKISLGIVETSKKNVIYK"
     gene            complement(5159..5275)
                     /gene="rrf"
                     /locus_tag="PKZ29_01700"
     rRNA            complement(5159..5275)
                     /gene="rrf"
                     /locus_tag="PKZ29_01700"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(5460..>5801)
                     /locus_tag="PKZ29_01705"
     rRNA            complement(5460..>5801)
                     /locus_tag="PKZ29_01705"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         1519 a         1566 c          765 g         1951 t
ORIGIN      
        1 gcaatttctg ccgccctggt ctgatcttcc ggtttttggt caaagcttga cgccaccaca
       61 tcacctttaa taaatctctg catatcagtt acttcttccg gtctttctcc caccaacact
      121 gccatcagat gagcttgagg ataatttttg gccactcccg ccgcaatttc ttttagcaaa
      181 gttgtctttc ccgcttttgg cggcgatacc accatacctc tttgtccaaa tcctaccgga
      241 caaaataaat ccaaaattct ggtcgacaaa atttcactct ctgtttccaa cttcatttgc
      301 tgatttgggt ataccggcac caagtcttta aattccaccc ggcgcaaagc cgacgccaaa
      361 tcaccctcat tgactttatc aattctcacc attgcccaat atttttcccc ttcttttggt
      421 tttttggctg ctcccgcaat catatctccg gctctcaaac cataccgcct aatctgcatc
      481 gaggcaataa atgcatcctt gccattgggc aaaaagtgcg gtctaacaaa tccgctgcca
      541 tcctgcatta tttccaacac ccccacaatt ggttctgtta ccaccgtttg agcataattt
      601 acagccggtc ttaccggcat tcttactggt gttggttttt ggaccgccgc cgctcccggc
      661 atcgctgttt gcggtttaac aaaactaacc gtgttattgc ttttggtttc tattatcgga
      721 gctttttctg gttccacttt tttaacttca cttatctccg tgacggtttt gtctattttt
      781 ggctccgcca ccccgccgct gattttcttt ctgggcattt atctaaaata aataataaat
      841 atcaactgtt taatcaatat aattggaaaa caacataaaa acgggaaatc gtttaaattc
      901 aaacccattc tacaaacttt ttatttcttt gtcaaataaa tccaaaacta tttactaatt
      961 aaacccaaag atagggttga aagtagcaga cctgccccct ctgctactct caaccctatc
     1021 ctcaagctaa attgaccaaa ttttaccaaa ttatcggcat tgccaaattt gactttatgt
     1081 caaaaaagct ttaatttaga tgttgaagaa acaacactat acgtgttcca cctgctttga
     1141 gaatatcgat aatgttgttt cttttttgtt ctttagctaa aatctcaagt caaagacgcc
     1201 gagattttgt ctaaagacca aactccgatc gaacaaagtg agtatcggag taattttccc
     1261 tcactcagtc gacatcggga taaatattcc acccattacc ttcgacaatg ttgtttctcc
     1321 aacttccaaa tttttaaatt gctttagcta aattagccta catcagtatc aattttcccc
     1381 ccaacttccc catttcccga atctgtctct ctgcccacaa actcccgtct cttttctttc
     1441 ctttcccttt gatcgatttt ttaatcgctc tctcagttga gaaaaatttt ttgattgact
     1501 gaagcattaa aaacagtata actcactttt gtcacttgtc tatatcctat aaaaatttag
     1561 tctctaaaaa ctaaacatcc tataaaattt tatttatgaa aattagcttc atttttaatc
     1621 attttttcct tttttgactc cttgtggaaa acttctggaa aactactccc cctccctatt
     1681 gcccaatttt gaccaaaaaa aatccggctt cccggattag taaataaaaa aaggggggag
     1741 ataaaatcga caaaaattgt tggctattaa gcctttgtca tattttatct ccccatagag
     1801 cctgtttctt aatctgtgtc tttttccttt ttctctttta cctctctttc cttttcctgc
     1861 tccgttagga tgtcttcaac ctcttgaagc acttctggtt tgtgtagatc ttccttcagc
     1921 attagaaaat aatgcctttt aacagaagac tttggctccc ttggatcgaa cactcggaaa
     1981 gacaccaacg tcaacggctt gttaggaaaa ttccaaaaac ctccttcttg agcgtcacgg
     2041 tcgattcccc aggcacccgc cattgtgatc ttctcaatcg tgaagttctt gaaatgatcc
     2101 ttaagtgcac ccactatcgt cgctgaagcc aaagatgttg ccatactcat ttttttcttc
     2161 ctcccttttc cttgtctcat ctttttaacc cgcttttggg ccaaaaaagt tacagctcta
     2221 ccacaattaa accccaaacc tcctttttca atgtacatcc aagtatatca cacattatag
     2281 gttatttcaa gccccgccaa atcccctcaa atcaattttt accaaatttc tgatatttat
     2341 cccgtctatt ttaattccct gacatttttt atctctacca tctttcttcc caacaattcc
     2401 gcttcttttt ttcttttttc ccagccgcgc acttttttta cctctcccag caaacttacc
     2461 tcccccacat acaccggcat ctttttgcct ccactactgt ctcccacaga aagtggcgcc
     2521 gatttatacg atgaccacat tgccgctaat accgccaaat ccgccccggt gtcctcaatt
     2581 ctaattcctc cgcttactgc cacaaacaca tcgcacttaa acaacggcat tcccaaactt
     2641 ttctgcccga tagctaccaa caactgccct cggttataat caatccccga aaacactctt
     2701 tttggcatcg gtgcaaaact ctccaccacc aatgcctgaa tttctaccaa catcggtcga
     2761 ctcccctcca aagttaccgt caccgctttg cccgccaaat tattttctcc tgatatcaaa
     2821 tttatctcct ctgacccaat ttctctcaaa cccctttcct ccattttaaa cacccccact
     2881 tcatccgtcg ctccaaatcg gtttttagtt gttcgcaaca ttcttaagtc gccatttttt
     2941 tctccttcaa aatacagcac cgtgtctacc atgtgttcca aaatctttgg tccggcaata
     3001 tccccctctt tggttacatg accgacaata aatatagtta tatttttttg tttggccagc
     3061 tgaaccaacc taaatgttgt ctccctaatt tgtcccaccg accccactcc tgcccgcgac
     3121 gacgatattg ttgactgact ctccgacacc attgtctgaa tactgtccac caccgctatt
     3181 ttgtatacat tatttactct atccaaatat tcctcaattt cttccactcg attagtttct
     3241 aaaatatcga atttttccgc atttaatccc aatctgtcca cccttagtcc gatttgttcc
     3301 gccgattcct cgcccgccac atacaagccg ccaactttac ccaccagttg ggtcaaaaga
     3361 gtgctttttc caatccccgg ttccccggca aacaacaccg ccgacccttg aacaattcct
     3421 ttacccaaaa ctctatcaaa ctccttaatt ccggtagaaa atatcaatgg agatttttgt
     3481 tttttatctg aattttgatt tatctccgac aaattcttta cctccacctt catcgctttc
     3541 ttaaatcttg aattttgtat tttagttcct tttatttctt tcaaactatt ccactgtccg
     3601 caagcgctgc actgaccctg ccactttgca tattcatttc cgcaattaga acacacaaac
     3661 atcgactctt tatttttcat tacccattat atcaactcta tcattctcaa aatcatcgtt
     3721 tctagcgcct ctgatgatat ctcaaaaaaa agacccgtaa actacgggtc tttttaaaaa
     3781 cactttctga tttatttata gatgacgttt ttcttgctgg tttctactat tcccaaagaa
     3841 atttttctct tttggacatc aatactctca atataaattt ggaccttgtc accctccttc
     3901 agattggtac cgccggtcaa tttggacaca tgaatcaatc cttccactcc tgtctccaat
     3961 ttaaccaaag ctccaaaatt ggctattcgc accacctcgc cttcagtttc tttgtccttg
     4021 ggatatttat cttcaatttt atcccacgga tcatttgtta atcgtttaat tgaaaattgc
     4081 agccgaccat catctttgtt aattagtttt atttttaatt tatccttaac cttaaacatg
     4141 gttgacaact cgctcacctt ttcccatgaa acctcagaaa tatgaaccaa tccttccaaa
     4201 ttcaacacct taccttcatt atcacaatta actctgacaa aaattccaaa aggttccacc
     4261 ctcataactt cagcttcaaa cacttcatcc aatttcaaat tttcgattgc tcccacttcc
     4321 tctcccacct tatccggttc cgacaccaat ctctctgaga aaactaatct atttttagcc
     4381 tggtctactt ccaaaacctt agtttttatt tttttgccaa tcattttgtc aggatcgccg
     4441 tcatatttag acccaatctg gcttccggga ataaaaccaa agaaaccaaa cggagcaatt
     4501 accaccgctc cgccccgatt aagttccttg gcaaataccg tcacctcccc cccgcttttt
     4561 tccttttcgc taaaataatt ccaaccataa cctgacgctg ctcctcgaat cgacaccaaa
     4621 atctgacctt tatcattttc cggttgtttt acctggactt caatttcatc tcccaccttc
     4681 aagtcattga tatagtcttt gacaaattca aactcttttc ccattaccac ggcatcggtt
     4741 tttcctccga tatcaatata aattgcctta tttttaataa ctgcaatttt tcccttaacc
     4801 tcctgccctt tcttaaaacc caattcctga attccttcca ttttcagcaa atctgccatc
     4861 gtcaatatct cattcttctt cgctgtctta gtttttttgg cctttttttc ttttttagtc
     4921 atattttaaa ctctgccctt cggcagaaca aaaaaatccc cattatttgg ggatatcacg
     4981 ggattgtcta ccctaaataa attattaata aaacatccgc tcttgagcgg atattcccta
     5041 tttattaaat caaaacttga agtcacaatt ttggaacgta tttgagcctg catcgcagac
     5101 tcattttcca gtggaaaaat tcgtgacaaa agttttaacc catcatttag tttctgagtt
     5161 ctggccatga cctattttca aatccccttg cgggaaaact attgtcagcg ctaaggcgtt
     5221 tcacttctct gttcgaaatg ggaaaaggtg gttccaccct gctccaatga ccagaattca
     5281 aaaactaaat cgagtttttt gtaattttat tttaatttca aactttataa acttgataaa
     5341 ctttcaactt tataaactaa tttcttggcc tctaaattga gtcgccctca gcttctagga
     5401 agcaaataca tgtgttttgc ttcttaaaag ccgaattctg taggcaaaaa gcctatctat
     5461 tgagaaattt cttcgatcat tccattgctt agctcaaacc cttacgggtc ttccacttgc
     5521 aaagtattgg ccggtagtct cccggcgatc ttccttcccg attgctcggg aaaacgattc
     5581 cttatcttag ggtcagtttc ctgtttgaga tgctttcaac agttatctta tagaaacggt
     5641 agctacccag cgatgcactt gacagcacaa ctggcacacc agaggtttcc tcatctaggt
     5701 cctctcgtac tgtagatgaa tcccttcaag aatcgaacga ttactacgga tagagaccga
     5761 gctgtctcgc gacgctctga acccaactaa cgcagcgctt t
//
LOCUS       DAOSHV010000031         5766 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_12293_length_5766_cov_5.420767, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000031 DAOSHV010000000
VERSION     DAOSHV010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 5766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5766
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12293_length_5766_cov_5.420767"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="PKZ29_01710"
     CDS             complement(<1..292)
                     /locus_tag="PKZ29_01710"
                     /inference="COORDINATES: protein motif:HMM:NF020118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NusA N-terminal domain-containing protein"
                     /protein_id="HOG37618.1"
                     /translation="MDLKKLPKTEFAAAVAQIAGERNIDVQSILDSIEFGLISAYKRD
                     QKERGAEVSEDDQFEVELSADSGSFKVFKLEGDKKIEVTPPGFGRIAAQTAKQ"
     gene            complement(449..973)
                     /locus_tag="PKZ29_01715"
     CDS             complement(449..973)
                     /locus_tag="PKZ29_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37619.1"
                     /translation="MRNILISQIKQSAADNAEKERREQEELEKKSAQQAKEREAYEKK
                     LQEQFLSDKQLLEETGYPDILRLIAACLESKDPEFLPLKEKIIKILTDNPHNQIADKR
                     NIHFSVSAATKSNYCIFFDVSWNNSKDILFTLSTTNKKVFLCMEKFQHQGIIEIPSRT
                     DQAAAEIIRFLMTV"
     gene            complement(1085..1942)
                     /locus_tag="PKZ29_01720"
     CDS             complement(1085..1942)
                     /locus_tag="PKZ29_01720"
                     /inference="COORDINATES: protein motif:HMM:NF013705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HOG37620.1"
                     /translation="MKKDFYEVLGVSKNATKNEIKSAYRKSALKYHPDRNKAPDAEDR
                     FKEINEAYEVLSNDQKKSAYDQFGHAAFDPNAGPFGGRTYTQQSGPFNFTYSQSGGNV
                     GGADFDFGGFSNPFDIFEQFFGAGFASQQSRGRRLETYKISLSFLEAANGVEKEFSID
                     GKRRKIKIPAGVDDGQRIRFKDFILYIDVLPDKIFKREGNDIFVTVNLTFSQAVLGTE
                     IKVPTLTEPVKVRVKSGTQPNTLIRLRGRGIKQVTGFGIGDLYIRLMVEIPTKLTSRQ
                     KDLVRELGL"
     gene            complement(1993..3903)
                     /gene="dnaK"
                     /locus_tag="PKZ29_01725"
     CDS             complement(1993..3903)
                     /gene="dnaK"
                     /locus_tag="PKZ29_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HOG37621.1"
                     /translation="MSNKIIGIDLGTTNSCVAVMEGGSPKIIVSAEGRNTIPSVVEPV
                     KKLVGDLAKRQMVLNSQNTIYSVKRLMGRRIDDPEVKKTQSVAPYKIVAGKDKMAAVE
                     VGGKSYTPQEISAMILQKCKTDAEKYLGEKVTDAVITVPAYFDDSQRQATKQAGEIAG
                     LNVQRIVNEPTAAALAYGMDKKQNELIAVYDLGGGTFDISILEIGDGIFEVKATNGDT
                     FLGGDDFDQKVITWIIDDFQKQNGVDLGKDPQALQRVREAAEKAKIELSSAAETEINL
                     PFITQKDGQPLHLTKKMTRSELEKLVDDLIQKTIVPVEKALKDAGVSKSDIKEVIMVG
                     GMTRMPKVQEVVKNFFGKDLNNNVNPDEVVAIGAAVQGAVLTGEVKDIVLLDVTPLTL
                     GIETAGGVRTPLIDRNTTVPTKKSQIFTTYSDNQPQVEINVLQGERPMAADNKSLGRF
                     ILDGIAPAPRGMPQIEVTFDIDSNGILSVSAKDKGTNKEQRITIQNATNLKEEEVEQM
                     KRDAEAHAREDEDKRLLAEAKNRLDGTIFSGEKTLKDLGDKIKPEDKEKIEAKIKESK
                     DVLAKTDVQIDELNKTSEELSQILQTVGAAIYQQQQPGPEGQQPSQDQPPQAEAEKSE
                     DTKEAEEGEVVQ"
     gene            complement(3918..4391)
                     /locus_tag="PKZ29_01730"
     CDS             complement(3918..4391)
                     /locus_tag="PKZ29_01730"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOG37622.1"
                     /translation="MTKSKKSQPNSTGPQTHQPHLSSRVAELEDRLKRSLADYANLEK
                     RIESQRQFFVTLATTAMVTKMIEVLDDFNLTYQHLQDAGLKIAIDKFMSVLKTEGVEE
                     IDALNRKFDPETMECIEAVCGQDNLVISVKKPGYKLNGHVIRPAQVAVGKADITN"
     gene            complement(4398..5135)
                     /locus_tag="PKZ29_01735"
     CDS             complement(4398..5135)
                     /locus_tag="PKZ29_01735"
                     /inference="COORDINATES: protein motif:HMM:NF013768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37623.1"
                     /translation="MADLTQRQIKILKCITEEFIETASPVGSETLEKKYSLGISSATI
                     RNEMATLTQMGFLKKGHLSAGRSPTSMGLKYYVRNLMTPKNLSASEEIGVKEKVWDYR
                     NEFDSLLRETTKELANRTRSMAIATTDHGVIYSHGASNLLEEPEFYNIDVTKTVLSLL
                     DNYGFWFDIINKASSVTDNDNDFVHLLIGEDLGIEHLEPCGFVYQKYEAGPHRGIVGV
                     VGPARFHYPHIVPIVDYFADLISKITR"
     gene            complement(5188..5478)
                     /locus_tag="PKZ29_01740"
     CDS             complement(5188..5478)
                     /locus_tag="PKZ29_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37624.1"
                     /translation="MNHKFFYFSLLIWSVFLWFINYVSPQFIWAKILFFVIIFAGFLS
                     LTLIFSRNYFLSFLISFYITLIFLLQSFRQLSIINLGITALLFGLVFFIFKK"
     gene            complement(5468..>5766)
                     /gene="rmuC"
                     /locus_tag="PKZ29_01745"
     CDS             complement(5468..>5766)
                     /gene="rmuC"
                     /locus_tag="PKZ29_01745"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HOG37625.1"
                     /translation="KKVILAGPFSFTAILRMVHQSYDNFRYQKNVANIIGYIRKFAEE
                     FDTYNQGFKDIGTKIETLQKQYHKVDSTRTNQLVKIVNKIKTEGGEPAKQLHES"
BASE COUNT         1585 a         1117 c         1315 g         1749 t
ORIGIN      
        1 cctgcttggc ggtttgagcg gcaatacgac caaaaccggg cggagtaact tcaatttttt
       61 tgtcgccttc gagtttaaaa accttaaatg agccgctatc ggcggaaagt tcaacttcaa
      121 attgatcatc ttcggagact tcggcaccgc gttctttttg gtcccgtttg taggcagaaa
      181 taagaccaaa ttcaatcgaa tctaaaatag attggacatc aatattacgc tcaccggcga
      241 tttgagcgac ggcggcggca aattcagttt ttggtagctt ttttagatcc ataaatctcc
      301 ttttttggcg atttattaaa aaaggggcct caccagcccc aaattcaaat cgcaaaaaat
      361 tatattagtg ggaatattat agcagaaaaa caaagaaaag cgcccatttg gttgtttttg
      421 acaaacccga tgggcgcgat aggtagattc agacagtcat aagaaacctt atgatttcgg
      481 cggctgcctg gtctgttctg gaggggattt cgataatacc ttggtgctgg aacttttcca
      541 tacaaagaaa tactttctta ttggtggtgc tcaaagtgaa gagtatgtct tttgaattgt
      601 tccaagaaac atcaaaaaag atacaataat tcgattttgt ggcagcagaa acagagaagt
      661 ggatatttcg cttgtcagcg atttggttgt ggggattgtc ggtaagaatt ttgattattt
      721 tttccttcaa gggcaaaaac tctggatctt tagattcaag acatgctgct atcagcctta
      781 agatatctgg atagccggtc tcttccaaca attgcttgtc ggaaaggaat tgttcttgaa
      841 gctttttttc ataggcttca cgttccttgg cttgttgcgc agactttttt tcaagttctt
      901 cttgctctct cctttctttt tcggcgttgt cggcagcaga ttgcttgatc tgagaaatca
      961 gaatatttct caaatccatg atacttccct ccttaaattt tttaggtgca cccgcttttg
     1021 ggttcaggct gattgtagca cggaaacaga attttatata ggatagattg ggtaattttt
     1081 taggctataa acctaattct ctgaccaaat ctttttggcg ggaagttagc ttggtgggga
     1141 tttcgaccat gagacggata taaagatcgc cgataccaaa gccggtgacc tgcttaatac
     1201 cacgaccgcg aagtcggata agagtgttgg gttgggtgcc ggatttgacc ctgactttga
     1261 caggttcggt taaagtagga actttaattt cggttcccaa aactgcctga gaaaaagtaa
     1321 ggttaacggt aacaaaaata tcattgcctt cgcgcttgaa aattttgtcg gggagaacgt
     1381 cgatatataa aataaaatcc ttaaacctaa tccgttgacc gtcgtcaact ccggcgggaa
     1441 tttttatttt ccgtcgtttg ccgtcgatac taaattcttt ttcgacaccg ttggcagctt
     1501 ctaaaaaaga gagactgatt ttgtaggtct ccaaacgcct gccgcggctt tgttgactgg
     1561 caaaaccggc accaaaaaat tgctcaaaaa tatcaaaggg attgctaaaa ccgccaaaat
     1621 caaagtcagc tccaccgaca ttgccaccgc tttgggaata agtgaaatta aacggaccgc
     1681 tttgctgggt ataggtgcga ccaccaaaag gaccggcgtt ggggtcaaaa gcagcatgac
     1741 caaattgatc gtaggcggat tttttttggt cgttgcttaa aacctcgtaa gcctcgttaa
     1801 tttctttgaa tctatcttca gcgtcaggag ctttgttgcg gtcgggatga tatttgagag
     1861 ctgatttgcg ataggcgctt ttgatttcgt ttttggtagc gtttttggag acacctaaaa
     1921 cttcataaaa atcttttttc ataaggtcaa ttatacttga taccccgccg aagcggggta
     1981 tgagttaatt agttattgaa caacttcgcc ttcttcagct tctttggtat cttccgattt
     2041 ttcggcttct gcttggggcg gttggtcttg agatggctgc tgaccttccg gtccgggttg
     2101 ctgctgttgg taaatagcgg cgccgactgt ttgtaaaatc tgactgagtt cttcgctagt
     2161 tttattcaac tcgtcgattt gaacatcagt tttagcaaga acatctttgg actctttgat
     2221 tttggcttca atcttttctt tgtcttcggg cttaattttg tcaccaagat ccttgagagt
     2281 tttttcgccg gagaaaatag tgccatcaag cctgtttttg gcttcagcca gaagacgttt
     2341 gtcttcgtct tcgcgggcat gagcttcggc atcgcgtttc atttgctcaa cctcttcttc
     2401 tttgagattg gtggcatttt ggatagtaat ccgttgttct ttgttggtgc ctttgtcctt
     2461 ggcagaaaca gacaaaatac cgttgctgtc gatgtcaaaa gtgacttcga tttggggcat
     2521 accgcgagga gcgggagcaa tgccgtccaa aataaaccga cccaaagatt tgttgtcggc
     2581 ggccataggc cgttcgccct ggagaacatt aatttctacc tgaggctgat tgtcggaata
     2641 ggtggtaaaa atttgggatt tcttggtagg cacggtggta ttgcggtcaa taagcggagt
     2701 gcggacacca ccggcagttt cgatacccaa ggttaacgga gtgacgtcca aaagaacaat
     2761 gtctttgact tcgccagtca agacagctcc ttgaacggca gcaccaatag cgacaacttc
     2821 gtcgggattg acgttgttgt tgagatcctt accaaagaag tttttaacaa cttcttggac
     2881 tttgggcatc cgggtcatac cgccgaccat aatgacctct ttgatatcgg atttggacac
     2941 accggcatct ttgagagctt tttcaaccgg aacgatggtt ttttggatta agtcatcgac
     3001 taatttttcc agttcagacc gggtcatttt tttggttaaa tgaagcggtt gaccgtcttt
     3061 ttgggtaatg aaaggcaagt taatttcggt ttcggcggcg ctggaaagct caattttggc
     3121 tttttcagca gcttcgcgga cccgttggag agcctgggga tctttgccca aatcgactcc
     3181 attttgcttt tgaaaatcat ctataatcca ggtaatgact ttttgatcaa agtcgtcacc
     3241 gccaaggaaa gtgtcgccat tagtagcttt gacttcaaaa ataccgtcgc cgatttcgag
     3301 aatggagata tcaaatgtgc cgccgcccaa atcgtagaca gcaataagtt cgttttgttt
     3361 tttgtccata ccgtaggcta aagcagcagc agttggttcg ttgacaattc gctgaacatt
     3421 gagaccggca atttcgccgg cttgtttggt ggcctgacgc tgagagtcgt cgaagtaggc
     3481 cggaacggta atgacagcat cagtaacttt ttcacccaaa tatttttcgg cgtcggtttt
     3541 gcatttttgt aaaatcatgg cagaaatttc ttgaggagtg taagatttgc caccaacttc
     3601 aactgcagcc attttgtctt tgccggcaac aattttgtaa ggggcgactg attgggtctt
     3661 tttgacttca gggtcatcaa ttcggcgacc cataagacgt ttgacactat aaatagtatt
     3721 ttgggagttt agaaccattt gccgtttggc aaggtcgcca accagttttt tgaccggttc
     3781 gaccactgaa gggatagtgt tgcgtccctc agcgctaaca ataattttgg ggctgccgcc
     3841 ttccataacg gcaacacagg aattggtagt tccaagatcg attccaatga ttttgtttga
     3901 catatttttt taattaatta atttgtaata tcggctttac cgacggcaac ctgagccggt
     3961 cggataacat gaccgtttaa tttgtaacca ggttttttga cagaaataac taaattatcc
     4021 tgaccgcaaa cagcttcgat acattccata gtttccgggt caaatttgcg gttgagagca
     4081 tcaatttctt ctaccccttc agttttgaga acggacataa atttgtcgat ggcaattttg
     4141 agaccagcat cctgaaggtg ttgataagta agattaaagt catctaaaac ttcaatcatt
     4201 ttggtaacca tggcggtagt cgccagagtg acaaaaaatt gacgctgtga ctcaattctt
     4261 ttttcgagat tggcatagtc agccaaagac cgtttgagtc tgtcttctaa ttcagccact
     4321 cttgaggata aatgtggttg atgggtttga ggcccagttg agttaggttg agattttttt
     4381 gatttagtca taaataatta acgagtaatt ttggaaataa gatcggcaaa ataatcgaca
     4441 ataggcacta tgtggggata atgaaatcgg gcggggccga caacaccaac gattccccgg
     4501 tgaggtccgg cttcgtattt ttgataaaca aaaccgcacg gttccaaatg ttcaattcca
     4561 agatcttcac caatcaaaag atgaacaaag tcattgtcgt tgtcggtaac agaagaggct
     4621 ttgttgataa tgtcaaacca aaaaccataa ttgtccagca aagacaaaac agttttggta
     4681 acgtcgatat tgtagaattc cggttcttca agcaaattag aagcaccgtg gctgtaaatg
     4741 acgccatggt cggtggtggc gatagccata gagcgggtgc ggttggctaa ttctttggta
     4801 gtttcccgaa gcagagagtc aaattcgttg cggtagtccc agactttttc tttgacgccg
     4861 atttcttcgc tggcggaaag atttttgggg gtcataagat ttctgacata gtatttgaga
     4921 cccatagaag taggagaacg cccggcagaa agatggcctt ttttgagaaa acccatttga
     4981 gtcagggtgg ccatttcatt tctaatagta gccgaagaaa tccccaggga atattttttt
     5041 tccagagttt cagaaccaac cggagaggcg gtttcgataa attcctccgt aatacacttg
     5101 aggattttta tctgtcgttg agttagatct gccatattag caatataata gatggactgc
     5161 taacagttgt caagggggat agataattta ctttttgaaa atgaagaaaa ccagaccaaa
     5221 aagcaaggcg gtgataccaa ggtttatgat agataattga cggaaagact ggagtaaaaa
     5281 aattaaagta atataaaaag agataagaaa agaaagaaaa tagtttcggg aaaaaataag
     5341 agtcaaagat aaaaatccag caaagataat cacaaaaaat aggatcttag cccaaataaa
     5401 ttggggagaa acatagttga taaaccacaa aaaaactgac caaatcaaca aagaaaagta
     5461 aaaaaattta tgattcatgg agctgttttg ccggttcccc accctcggtt ttaatcttat
     5521 tgacaatttt gactaactga ttggttctgg tggaatcaac cttgtgatac tgcttttgga
     5581 gagtttctat tttggtgccg atatccttaa aaccctgatt gtaggtgtca aattcttcgg
     5641 caaattttct gatgtaacca atgatattgg cgacattttt ttgataacgg aaattgtcat
     5701 aagactggtg taccatacgc aaaatggcag tgaaactgaa agggccggct aaaataactt
     5761 ttttgg
//
LOCUS       DAOSHV010000032         5698 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_12490_length_5698_cov_6.001418, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000032 DAOSHV010000000
VERSION     DAOSHV010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 5698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5698
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12490_length_5698_cov_6.001418"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /gene="infC"
                     /locus_tag="PKZ29_01750"
     CDS             complement(<1..400)
                     /gene="infC"
                     /locus_tag="PKZ29_01750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOG37626.1"
                     /translation="MSSFRNRNTSNKFYKINQYITAPQVRLLDEHGKQIGVVSIAEAR
                     SMSQESGLDLIEINGKAVPPVIKLISFSKFKYQEAKKLQSEKKGIKGGELKEIQMTPF
                     IGQNDYQIRVNKAKKFLATGNKIKLSIKFQG"
     gene            complement(426..2192)
                     /gene="thrS"
                     /locus_tag="PKZ29_01755"
     CDS             complement(426..2192)
                     /gene="thrS"
                     /locus_tag="PKZ29_01755"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOG37627.1"
                     /translation="MNSNSELQSLRHSAEHILTQAMEHLYPKKFLMAMGPATDDGFYF
                     DFEPLKDFKISEADFPQIEAEMTKIVKQNLPIVKKEIPLDEAKKIFKHNPYKMEWLNS
                     IKDRGEVITVYQTGDDFIDLCAGPHVRYTSKVKAFKLLSIAGAYWHGDEKNKMLTRIY
                     GTAFESKEDLQKYIDLMVEIQKRDHRKLGKDLGLFTFSDLIGSGLPLYTPKGAFIRLA
                     LNNYVESVQTKQGYTQVWTPQIAKAKLFQTSGHYDKYKDSMFRVVSNYSEEEFFLKPM
                     NCPQHTQIYSAEPHSYRDLPLRFADFAMLYRDEKPGELNGLARVRGFSQDDCHIFCTE
                     EQVDQEIDTALKMTKEIMDTFGLKYKYRLSTRDPDHPEKYLGDPKIWDKVEKWAVDIM
                     KRNKIEYFDGPGEAAFYAPKMDLIATDALGREWQLSTVQIDYVMPERFGLEYTAADGT
                     KKRPVMLHRAIVGSPERFLMIMLEHFGGALPTWISPVQIKIIPITDNQLEYAKKIESE
                     LKDNNIRVEIDDKSETMQNKIRTATGEKIPYLIIVGGREADNNTISVRQRDGQDLGAM
                     QLADFMSQIKDQITNKALNLIK"
     gene            2252..3691
                     /locus_tag="PKZ29_01760"
     CDS             2252..3691
                     /locus_tag="PKZ29_01760"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOG37628.1"
                     /translation="MREKFKNFKIVNWIVVLVIVLGAGLRLKNFSVPPVDGHQMRQTD
                     TECVAYFLYSGKANFFRPKSCLMRPVSNTKGYFFLEMPFYESLIAISYKIFGPEIWAA
                     RLVNLLLYIIGAVALWKFLGGWIDKKVGLWGLIIYSLMPGSIFFVGHAIHPDAMAVSL
                     IWVSLYLIYKFSQNNNFKFLIGAGVAMGISVASRPFGLIALPLLVYFLWERKSKWWNY
                     LVLIFLSIVFYGWWRWWTHFLNLDMSWENWVLHGREKLFDLGILKNLIWKNVVGEVMG
                     KTASALAVLGWLVGFLKKNRKMLPLGWWTLGVVVYWLMVPNGNLTHQYYADVYIPLVV
                     ILAAIGWNFIFEKSKILGILVLPILVWNGVRVSNYFLVPYIEAQKDIEIAQEIKQEIG
                     EDKKIIYLNRVNSVPLSLSHRQGWILGEWPTDVAGHIWAFMEMRNFKFDYIVEPKHKV
                     DLKTEDWEIIKQNYPLVKFGEKINIFERR"
     gene            complement(3693..3766)
                     /locus_tag="PKZ29_01765"
     tRNA            complement(3693..3766)
                     /locus_tag="PKZ29_01765"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3730..3732),aa:Val,seq:gac)
     gene            complement(3790..4365)
                     /locus_tag="PKZ29_01770"
     CDS             complement(3790..4365)
                     /locus_tag="PKZ29_01770"
                     /inference="COORDINATES: protein motif:HMM:NF039817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5660 domain-containing protein"
                     /protein_id="HOG37629.1"
                     /translation="MAGGLIFKPSSKSKNLVKKNTSEILKDFPDNQAPVQKKSGILFP
                     GQSIRLNSSESKPSQINWSHEFLTKPITQEQSLFVNQRLQETNTAVEELRREIKSLIK
                     STDNLQAEITNTVDQNIVEASQYQVNFLTRIKNLIINFRQNIDQSCLWLESFNRKKNR
                     KNAFWGTVKNKKSGGEQYLLSGEHSVSRSAN"
     gene            complement(4388..5197)
                     /locus_tag="PKZ29_01775"
     CDS             complement(4388..5197)
                     /locus_tag="PKZ29_01775"
                     /inference="COORDINATES: protein motif:HMM:NF017508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOG37630.1"
                     /translation="MTPNSSIQKLKDKSFILNLEIKVEDIRQEYQKTLKSIQLNFETK
                     GFRKGKAPLDVVESQISPQKIFEEVATNLISYQYSDAVKDHHLRPITQPQIKFKTKNP
                     DLEHSWQIEITGSELPKISLNQGYLKEIKSINQDKSIPDDNAKLDKIIQALVKHSKLD
                     LPEILIQSDLQHHLSHLIDQANQAGITVQQYLESHKQTLENYQDELKKRIVQEWTINL
                     AITQIAKDNKIEVSESETKDLLAKNPSLSQNTNMLYYLLTQQKVFEFLKKQ"
     gene            5228..>5698
                     /locus_tag="PKZ29_01780"
     CDS             5228..>5698
                     /locus_tag="PKZ29_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37631.1"
                     /translation="MERLINKFKKVIISVSLMVFGVLVFFLELNYLGSVNNKRNVFGA
                     VSENRVLDLTIVGSEDNVEQIKVKEEVSGDDARPVIIKKYLERYKSPLAPYSDLIYQI
                     SNDYGFEYYWIVAIAQQESNLCKKIPEDSHNCWGYGIHKNGTLKFENYELAIKSY"
BASE COUNT         1626 a          814 c         1299 g         1959 t
ORIGIN      
        1 gaccttggaa ttttatactt aatttgattt tatttccagt agctaaaaat tttttggctt
       61 tgttaacccg gatctggtag tcattttgac ctataaatgg tgtcatttgg atttctttta
      121 gttcgccgcc tttgatcccc ttcttttctg attggagttt tttggcttcc tgatatttga
      181 atttggaaaa actaataagt ttaatcaccg gagggacagc cttgccatta atttcgatta
      241 aatccaagcc tgattcctga gacatagatc tggcttcggc aatggagaca actccaattt
      301 gtttgccgtg ttcgtccaaa agacgaactt gcggagcagt aatatattgg ttgattttat
      361 agaatttatt gctggtattt ctatttctaa aactactcaa ggtaataata atttgtaatt
      421 attggttatt ttatcaaatt taaggcttta tttgtgattt gatctttgat ctgagacata
      481 aaatcggcaa gttgcatagc tcccaaatct tgaccatcgc gttgacgaac ggaaatagtg
      541 ttattgtcag cttcccgacc accgacgata attaggtaag ggattttttc accggtggca
      601 gtgcggattt tgttttgcat ggtttcggat ttgtcatcaa tttccactct aatattgttg
      661 tcttttaatt cagattcaat ttttttggca tattctagtt ggttgtcggt aatgggaatg
      721 attttaatct gaacaggaga gatccaagtt gggagagcgc caccaaaatg ttctagcata
      781 atcattaaaa atctttccgg tgaaccaact atagctcggt ggagcataac aggtcgtttt
      841 ttggtgccat cagcagcagt gtattccaat ccaaaacgct ctggcataac ataatcaatt
      901 tgaactgtgg atagttgcca ttcgcgacct aaagcatcgg tggcaattaa atccattttt
      961 ggggcataga aagcagcttc tccaggtccg tcaaaatatt caattttgtt tcttttcata
     1021 atatcgacgg cccatttttc aactttgtcc caaattttgg gatcgcctaa atatttttcc
     1081 ggatggtcgg ggtcacgagt agataagcga tatttatatt taagaccaaa agtatccata
     1141 atttctttgg tcatttttag agcggtgtcg atttcctgat caacctgttc ttcggtgcaa
     1201 aagatgtgac agtcatcttg agaaaagccg cgaacgcggg ctaatccatt taattcaccg
     1261 ggtttttcgt cacgatataa catagcaaaa tcggcaaatc ttagtggtaa atcacgatag
     1321 ctgtggggtt cggcgctgta gatttgagtg tgttggggac aattcatggg tttgagaaaa
     1381 aattcttctt cagaatagtt gctgacaacc ctaaacatac tgtctttgta tttatcataa
     1441 tgacctgaag tctggaataa tttagctttg gcaatctgcg gtgtccagac ctgggtataa
     1501 ccctgtttgg tttggactga ttcaacataa ttattgagag ctagtctgat aaaggcacct
     1561 tttggggtgt ataaaggcaa accactaccg atcaggtcag aaaaagtaaa taaccccaag
     1621 tctttaccta attttcgatg atcccttttt tggatttcta ccatcaaatc aatgtatttt
     1681 tgaagatctt ctttagattc aaaagcggtg ccgtaaatac gagtaagcat tttgtttttt
     1741 tcatcgccgt gccaataagc gccagcaata gatagaagtt taaaggcttt gaccttgctg
     1801 gtatagcgaa cgtgcggtcc ggcgcaaaga tcaataaagt catcaccggt ttgataaacg
     1861 gttataactt ctcccctatc tttgatagag tttaaccatt ccattttgta gggattatgt
     1921 ttaaaaattt ttttagcttc gtccaggggt atttcttttt tgacgatggg taaattttgt
     1981 ttgacaattt tggtcatttc agcttcgatt tggggaaaat cagcttcgct gattttgaag
     2041 tctttaagcg gctcaaaatc aaaataaaaa ccatcatcgg tagctggacc catagccatt
     2101 agaaattttt tgggatagag atgttccatg gcttgagtca aaatgtgctc agccgagtgg
     2161 cgaagagact gtaattcaga attagaattc atggcttatt ttagcagaaa cagctttggt
     2221 ttttgcaaat gaaaatttgt aaaatagaat ggtgagagaa aaatttaaaa attttaagat
     2281 tgttaattgg atagtggttt tggtaatagt tttaggagca ggattgaggc ttaaaaattt
     2341 ttctgttcca ccggtggatg gtcatcagat gagacaaaca gatacggagt gtgtagctta
     2401 ttttttgtat agcggtaagg ctaacttttt taggccaaaa agttgtttga tgagaccagt
     2461 gtcaaatact aagggttatt tttttctgga aatgccgttt tatgagagtt taattgccat
     2521 aagttataaa atttttggac cggaaatttg ggcggcaaga ttggtgaatt tacttttgta
     2581 tataatcggg gcggtggcat tgtggaagtt tttgggtggt tggatagata aaaaagtggg
     2641 gttgtggggg ttgataattt acagtctaat gccgggaagt attttttttg tgggtcatgc
     2701 aattcacccg gatgcaatgg cagtaagttt aatttgggtg tcactatatt taatttataa
     2761 atttagtcaa aataataatt ttaaattttt gattggggcg ggggtggcga tggggattag
     2821 tgtggccagc aggccatttg gattgattgc tttgccgctt ttggtttact ttttgtggga
     2881 gagaaaaagt aaatggtgga attatttagt attaatattt ttgtcgatag ttttttatgg
     2941 ctggtggcgg tggtggactc attttttgaa tttggatatg agttgggaaa attgggtgct
     3001 gcacggcaga gaaaaattat ttgacttggg gatactgaaa aatttaattt ggaaaaatgt
     3061 ggtgggtgag gtgatgggga aaacagcaag tgcattggcg gttttggggt ggttggtggg
     3121 gtttttaaaa aaaaatagaa aaatgctacc gctgggatgg tggacactag gggtagtggt
     3181 ttattggcta atggtgccga atggaaattt aactcatcag tattatgctg acgtttatat
     3241 accgctggtg gtgattttgg cggctatagg gtggaatttt atttttgaaa aaagtaaaat
     3301 tttgggaatt ttagttttgc cgatattagt gtggaacgga gtgagggtaa gtaattattt
     3361 tttggtgcct tatattgagg ctcaaaaaga tatagaaatt gctcaagaaa taaaacaaga
     3421 gataggagag gataaaaaaa taatttatct aaatcgggtg aattcggtgc cattgtcttt
     3481 gtcacaccga caagggtgga tattgggaga gtggccgaca gatgtggcag ggcatatttg
     3541 ggcatttatg gaaatgagaa attttaagtt tgattatatt gtggagccaa aacacaaagt
     3601 agatctaaaa actgaagatt gggaaattat taaacaaaat tacccgctgg taaaatttgg
     3661 agaaaagata aatatttttg aaaggagata agtgagtcta agaggactcg aacctctgac
     3721 ctcttcgatg tcaacgaagc gctctaacca actgagctat agactcgttt gatagccaaa
     3781 attatatcat tagttggcag aacgagaaac actgtgttcg ccactgagaa gatattgttc
     3841 tcccccactc tttttgtttt tgacggtacc ccagaaggcg tttttacggt tttttttgcg
     3901 attaaaactt tctaaccaga gacaagactg atcaatattc tgtctaaaat taataataag
     3961 gtttttgata cgggtgagaa aattgacttg gtattgactg gcttcaacaa tattttgatc
     4021 aacggtgtta gtaatttcag cctgaagatt gtcggtgctt ttaatcaggc tttttatttc
     4081 ccggcgaagt tcttcgacag cagtattggt ttcttgcagt cgttggttga caaaaagaga
     4141 ctgttcctga gtaatcggct tggtgagaaa ctcgtgactc cagttgattt gggacggttt
     4201 tgattctgaa gaattgagac gaatactttg accagggaaa agaataccag attttttttg
     4261 aacaggtgcc tgattatcgg gaaaatcctt gagaatttcg ctggtatttt ttttgactaa
     4321 atttttggat ttagagctgg gtttaaatat tagtccacct gccatacctg attattttag
     4381 cacaaaacta ctgttttttt agaaattcaa agactttttg ttgagtcaaa aggtagtaga
     4441 gcatgttggt gttttgggat aaactggggt tttttgccag taaatctttg gtttcggact
     4501 cagaaacttc gattttgttg tctttggcaa tttgggtgat agccagatta atggtccatt
     4561 cctgaacaat tctttttttg agttcatctt ggtaattttc cagagtttgt ttatgggact
     4621 ctaagtattg ttggacagta atgccggctt gattggcttg atcgattaga tgagaaaggt
     4681 ggtgttggag atcggattgg attagaattt cgggtaaatc tagttttgaa tgtttgacta
     4741 gggcttggat aattttgtcc aatttggcgt tgtcatcagg aatggattta tcttggttga
     4801 tagattttat ctccttgaga taaccctggt ttaaactgat tttgggcaat tcactaccag
     4861 taatttcaat ttgccagcta tgctcaagat cgggattttt ggtcttaaat ttgatttgag
     4921 gttgagtaat aggacgaaga tgatggtctt tgacagcgtc ggaatattga taagaaatta
     4981 agttagtggc gacttcttca aatatttttt gaggtgaaat ttgggactca accacatcta
     5041 gaggagcttt gcctttgcga aaacctttgg tttcgaagtt taattgaatt gattttagag
     5101 ttttttgata ctcctgtcgg atatcttcga ccttgatttc taggttgaga ataaaagatt
     5161 tatccttaag tttttgaatt gaagagtttg gtgtcataag tgtgcttatg ataccataga
     5221 aacaggaatg gaaaggttaa taaataaatt taaaaaagtc ataatttcgg tatcattgat
     5281 ggtttttggg gtattagtat tttttttgga attgaattat ttgggaagtg ttaataataa
     5341 acgaaatgtc tttggggcgg taagtgaaaa cagggttttg gacctaacaa tagtcggatc
     5401 ggaagataat gtcgagcaaa ttaaggttaa agaagaagtg tccggcgatg atgccagacc
     5461 ggttattatc aaaaaatact tggaacgata taaatcacct ttagccccct actctgactt
     5521 gatttatcaa atttctaatg attatggttt tgaatattat tggatagtcg ccatagctca
     5581 acaagaatct aatttgtgta aaaagatacc tgaggattct cataattgct ggggctatgg
     5641 aattcataaa aatggaactt taaaatttga aaattatgag ctagccataa aaagctat
//
LOCUS       DAOSHV010000033         5588 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_12851_length_5588_cov_4.090909, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000033 DAOSHV010000000
VERSION     DAOSHV010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 5588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5588
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12851_length_5588_cov_4.090909"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            101..589
                     /locus_tag="PKZ29_01785"
     CDS             101..589
                     /locus_tag="PKZ29_01785"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOG37632.1"
                     /translation="MKKHNLRIKNSSGFTLIEILVVIALIGILASIAAVMFGAARDKA
                     RTASAMASMKSMITEAALHQDSDGRFPSDLCDGQGVPLDYGHLLTGDLQRLSVAVYDQ
                     LKYHPACSIDVAFGDKPSAWAAFVDVDPTETTQNFCVDSSGFSGIGYKAGAGLGECIQ
                     PL"
     gene            734..2647
                     /locus_tag="PKZ29_01790"
     CDS             734..2647
                     /locus_tag="PKZ29_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37633.1"
                     /translation="MNKKAFLGLVFSIVVIMAAMAYLPFPVGPVGAQDTGTVDNELRG
                     WAWSDSTGWVSFNHEGTDGMSSSGGTSSGSTEYKMPSSATQKIKAPGSLTWVSPILSL
                     NEDGTFTTAYIEDDSQSTNYLENRGFGLSLPAGANVTGIEMCYKRASEQSGLADTRVH
                     DENIQLIKPNGSYSLDNKSQGTQWSENQLTELGWVCYGGPGDTWGESWTSTDIANPYF
                     GTVISASLTADRNGSGKITLYIDAVKVKVYYDEPAGSPASSGSDYAVMVDDDGNLTGY
                     AWSLNLGWIKFEPGSSFAPSDCNTGDCYGAKIVERGDVDEVKGWARACSVFQSGCSGA
                     LRPNSQLGGWDGWIKMHNVKRQANGGFYGYAWGDLNLAWLDLGFGVGDDGSGDEVCDP
                     TEQVCGGGGEICDPDTECCPSEEGYPDCNDGDSQVSVVVNSECGSELGIEWNPSGDEC
                     GEFCRSYSQGERVEVTISGTPEGSNLIVSGDCNSNPCVFDSIDGNKAITVSCPAIDIS
                     DCQLKLTGGSILQLIEDSYLDYNDVVLSDTFAKISLININTNAACGVNYDLSSQTGDS
                     NVSILCESGGTYGSCNGLNGSGETKIKARFMTRPDEANETWNVIFVASSGGVSSLPKS
                     YNSLIRYYRRIGN"
     gene            2647..3438
                     /locus_tag="PKZ29_01795"
     CDS             2647..3438
                     /locus_tag="PKZ29_01795"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HOG37634.1"
                     /translation="MLLAYCLVFILGLIVGSFLNVIVLRLHSGESVVFDRSRCLSCGK
                     KLNWYELIPVFSFLIQGGHCRGCHSKISWQYPLVELLTGLLFVLSFWRFASDGWLFIL
                     FYLIIVSLSIAITVYDLRHRIIPDVLVWLMAVLALFRPLLLFLSDRDLPSLISDYQTV
                     VLGGLAAGGVFFLLWLISRGRWLGFGDVKLALVVGFLLGPIQVFSALILAVWLGAVVG
                     LILVILTKTGLFGFRKYFTIKSELPFAPFIILGLILNLLFKINVF"
     gene            3428..3958
                     /locus_tag="PKZ29_01800"
     CDS             3428..3958
                     /locus_tag="PKZ29_01800"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOG37635.1"
                     /translation="MFSKKSQQGFSLLELMVVIAIFLIITAVVMADIPGIGRKGALDL
                     TAQEVAGCVRAAQVYGTAKKIELTNTNDIIGAKVEQDKIIIFANDLSSENNIIETCEL
                     NGFNLTSEDFDIVKFPSSLYERTIQTSLEPEFYNGATPSLPSSAFEIKVTSIRTNQVK
                     CVYIYQSGQITVGDCE"
     gene            3955..4494
                     /locus_tag="PKZ29_01805"
     CDS             3955..4494
                     /locus_tag="PKZ29_01805"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOG37636.1"
                     /translation="MKNHKRQINKGFTLVEMLVSVSIFVIVALIVSGVLVQLSVAYKK
                     AQAMRLLMDNLNFALEKMNLEIREGVSIDTFSDDSAIQFSQIGDSTVFCYSPLNFNER
                     NTLVKCESPCPCSETSPNLKDMLSSGLELSGLRFQKFGPNRQERVLINIAGSARSSAN
                     QVTDFVIQTTATQRIQDPS"
     gene            4508..4987
                     /locus_tag="PKZ29_01810"
     CDS             4508..4987
                     /locus_tag="PKZ29_01810"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOG37637.1"
                     /translation="MKKKNKGFTMVEALVAISILLIAVVGPLSLLATALRDSVYIRNE
                     ITANYLAQEGLEIITFFSDEISSGNYCVDATGDDYGDAVNSSSDCPIKYDGNYYGGNG
                     ENTIFNRVIEVERLRDEETMADDYDGSELRVTSRVSWQNQGFSVPKDISYTTYIFKK"
     gene            4984..5532
                     /locus_tag="PKZ29_01815"
     CDS             4984..5532
                     /locus_tag="PKZ29_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37638.1"
                     /translation="MKKNNNNKGFALLYAVLLTSAVLIVGVTLINIITRQLILSSLNR
                     NSQIAYYNANSIANCFRFYDKVNHFTALDFDSLSFTYVNNIDLQCAGGVVKEDLNFGG
                     NEISDVSFEINGQVTTIADFILNLGYINFPDTYNSNNCFVPASGEGFSNSYISVITVN
                     GSNTQIGSSNPRKVIRTAISIR"
BASE COUNT         1532 a          924 c         1346 g         1786 t
ORIGIN      
        1 ttacgcttct gttttgggat tagtttggta aaatgtaaga caggataaga aagataggaa
       61 aaaggtgcac ggaaacggac aaaaaaaata agcaacactt atgaaaaaac acaatttgcg
      121 gattaaaaac tcttccggct ttactttgat tgaaatcttg gtggtgattg ctcttatcgg
      181 tattttagcg agtattgccg ctgtgatgtt tggcgcggcg cgagacaagg cgcgtaccgc
      241 ttcagcgatg gccagtatga aatcaatgat tacggaggcg gctctgcacc aagattccga
      301 tggaaggttt ccaagcgatt tgtgtgatgg tcaaggcgtg cccctagatt acgggcacct
      361 tcttactggg gaccttcaaa gactttcggt ggcggtatat gaccaattaa aatatcaccc
      421 ggcttgttct attgatgtag cttttgggga caaaccttct gcttgggccg cctttgttga
      481 cgttgaccca actgaaacaa ctcaaaactt ttgcgttgat agcagtggtt tttccggaat
      541 agggtacaag gcaggagcag gattgggtga atgtatccaa ccgctgtaac aaacggccca
      601 ataacctcaa tccttgtttt ggtcctaaag tggataactt ttgacaaaaa tagcggggtt
      661 ttgtgttaaa attgggataa cttttaatac ataaaattaa ttaaaataca caaaatttta
      721 tatcttttaa actatgaata aaaaagcatt tctcggtttg gtattttcta tcgtagttat
      781 tatggccgct atggcttatt tgccgtttcc ggttgggcca gtgggcgcgc aggatactgg
      841 aacggtagat aatgagttgc gcggctgggc ttggagcgac agcaccggct gggtgagctt
      901 taatcacgag ggaaccgacg gaatgtcttc ttcgggcgga acgagttctg gtagcacgga
      961 atacaagatg ccaagttcgg ccactcaaaa aataaaagcc cccggctctt taacctgggt
     1021 ttctccgata ctttctctta atgaagatgg aacgtttacc actgcttata tagaagatga
     1081 ctcacagagc actaattatt tggaaaacag gggttttggt ctaagtctgc ctgccggcgc
     1141 aaatgtaacc ggaatcgaaa tgtgttacaa gcgagcaagc gaacaaagtg gtctcgctga
     1201 tacaagagta catgatgaaa atattcaatt aataaaacca aatggttcat attccttaga
     1261 caacaaatct caaggtacgc agtggtctga aaatcaactt acagaattgg ggtgggtttg
     1321 ttatggaggg cccggtgata cctggggtga aagttggacc tcgaccgaca ttgctaaccc
     1381 ctactttggt acggtgatat cagctagttt gacggcggac aggaatggga gcgggaaaat
     1441 caccctttac atagacgctg ttaaggttaa ggtttattat gacgagccgg ccggaagtcc
     1501 ggcgtctagc ggttcagatt acgcggtaat ggtggatgat gatggaaatt tgaccggtta
     1561 tgcgtggagt ttgaatttgg gttggattaa atttgaaccg ggttcttctt ttgcgccatc
     1621 tgattgtaat acgggtgatt gttatggtgc aaaaattgtt gagagaggag atgttgatga
     1681 ggtcaaaggc tgggctaggg cttgttcggt ttttcagagt gggtgtagcg gcgctttacg
     1741 gcccaattct caacttggcg gttgggatgg ctggattaaa atgcataacg tcaaaagaca
     1801 ggccaacggt ggtttttatg gttatgcttg gggtgatttg aacttggcgt ggctcgacct
     1861 cggttttgga gttggagatg atggcagtgg tgacgaggtt tgtgatccaa ccgaacaagt
     1921 ttgtggcggc ggcggtgaaa tctgtgatcc ggatacagaa tgttgtccga gcgaagaagg
     1981 atacccagat tgtaatgacg gcgacagcca agtaagtgtg gttgttaata gtgaatgtgg
     2041 gtcggaattg ggaatagagt ggaatccttc cggagatgaa tgtggtgaat tttgtagatc
     2101 atattctcaa ggtgaaaggg tagaagtgac tatcagtgga acaccggaag gcagcaactt
     2161 aattgtttcc ggtgattgta actcaaatcc ctgtgtgttt gatagtattg atggcaataa
     2221 agcgataact gtcagttgtc cggctattga tattagtgat tgtcaattaa agctgactgg
     2281 cggtagtatt ctgcagttaa ttgaagattc ctacctcgat tataatgatg ttgtgttgag
     2341 tgatactttt gccaagattt cactaataaa tatcaatacg aatgctgctt gtggtgtaaa
     2401 ttatgatttg tccagtcaga caggtgattc aaacgtctct atcttgtgtg agtccggggg
     2461 aacttatggc agttgtaatg gtttgaatgg ctcaggggag acaaaaatta aagctcgctt
     2521 catgacaagg ccagatgagg caaacgagac atggaatgtt atttttgtag cctcatcagg
     2581 cggtgtttca agccttccca aatcttataa ttcgctaatt cgttattata ggcgaatagg
     2641 aaattaatgt tacttgccta ttgtttagtt tttattctgg ggcttattgt tggcagtttt
     2701 ttgaatgtga ttgttttgcg tctgcattcc ggcgagtcgg tggtttttga ccgttcgcgt
     2761 tgcctttctt gtggcaaaaa acttaattgg tatgagctaa ttcccgtttt tagtttttta
     2821 attcagggtg gtcattgccg cggctgtcat tccaaaattt cttggcagta cccattagtg
     2881 gaacttttga ctggtctgct ctttgtttta agcttttggc ggtttgcgtc agacggttgg
     2941 ttatttatat tgttttatct cataattgtt tcgctttcta ttgccataac tgtctatgat
     3001 ctaagacata gaattatacc ggatgttctt gtttggttga tggcggtcct ggccctattt
     3061 cgcccacttt tattgtttct ttctgatcgc gatcttcctt ctttgatttc tgattatcag
     3121 acagtcgttc tgggtggttt agccgccggt ggcgtctttt tcctgttgtg gctcatctcg
     3181 cgcggtcgat ggcttggttt tggcgatgtc aaactggctt tggtggttgg ttttttgctc
     3241 gggccgattc aagttttttc ggctttgatt ctggcagtct ggcttggcgc ggtggttggg
     3301 ctcattctag ttattctgac caagactggg ttgtttggat ttagaaaata ttttacaatt
     3361 aagagtgaac ttccttttgc gccgtttatt atattgggtt taatcttaaa cttgcttttt
     3421 aagatcaatg ttttctaaaa aatcacaaca aggtttttca ctacttgagc tgatggtagt
     3481 catcgcgatt tttttgatta tcacggcagt ggtgatggcc gacattcccg gtataggtcg
     3541 caaaggggca cttgatttga cagctcaaga ggtggcgggg tgtgttcgag cggctcaggt
     3601 ttatggtacc gccaaaaaaa ttgaattaac aaacactaat gatattatcg gggccaaagt
     3661 tgaacaagac aagattataa tttttgccaa tgatttaagt agtgaaaaca atataattga
     3721 aacctgtgag ttaaatggct ttaatctcac atcggaagat tttgatattg ttaaatttcc
     3781 cagttctcta tacgagagaa cgattcagac aagtttggaa ccggaatttt ataatggcgc
     3841 aacgccttct cttcccagtt cagcttttga aattaaagtg acttcaattc gtaccaatca
     3901 ggtaaagtgt gtatatattt atcaaagtgg tcaaataaca gttggagatt gtgaatgaaa
     3961 aatcataaaa gacaaataaa taaagggttt acgttggtgg aaatgttggt ttccgtttca
     4021 atttttgtga tagtagcttt gattgtgagt ggggttctag ttcaactctc ggttgcttat
     4081 aaaaaggcgc aggccatgag gttgttgatg gacaatctta actttgcttt agaaaaaatg
     4141 aatctggaaa taagagaggg ggttagtatt gacacttttt ccgatgattc ggcaattcaa
     4201 tttagtcaaa taggcgactc aacagttttt tgttactcgc ctttgaattt taacgaaaga
     4261 aataccttgg tcaaatgtga aagtccttgt ccttgctctg aaacaagtcc gaatttaaaa
     4321 gatatgttat caagtggttt ggaattgtca ggattgcgtt ttcaaaaatt tgggccgaac
     4381 cggcaagaaa gagttttgat taatattgcc ggtagtgcca ggagtagcgc caatcaagta
     4441 actgattttg ttattcagac gactgctacc caaagaatac aagacccgtc ttaatatgaa
     4501 ttattcaatg aaaaaaaaga acaaaggatt tacaatggtt gaggctttgg tggcaatcag
     4561 tattttattg attgccgtgg ttggcccact ctctcttttg gcaacagcct tgcgtgatag
     4621 tgtatatatt agaaatgaga ttacagccaa ctatttagcc caagaaggtt tggaaataat
     4681 aacttttttt agcgatgaaa tcagttctgg aaattattgt gttgatgcga cgggggatga
     4741 ttacggtgat gccgttaatt catcttccga ttgtccaata aagtatgacg gaaattatta
     4801 tggaggtaat ggtgaaaaca ctatttttaa tcgtgtaatt gaggtagaaa gattgaggga
     4861 tgaagagact atggctgatg attacgacgg tagtgagtta agggtaacct caagagttag
     4921 ttggcaaaat caaggtttct cagttccaaa agacatttct tataccacgt atatatttaa
     4981 aaaatgaaaa aaaataataa caacaaaggt tttgctctac tttatgctgt tcttctgaca
     5041 agcgctgttt tgattgtcgg agtaactttg attaacatca tcacgcgtca actaatacta
     5101 tcttctctaa acagaaattc gcaaattgct tactacaatg ccaactcaat tgctaactgt
     5161 tttcgttttt atgataaagt taatcatttt acggctcttg atttcgattc tttaagtttt
     5221 acttatgtca ataatataga cttacaatgc gctggaggtg ttgtaaaaga ggatcttaat
     5281 tttggaggta atgaaataag tgatgtgagc tttgagataa atggtcaagt aaccactatt
     5341 gccgatttta tccttaatct tggatatata aattttccag atacctacaa ctcaaataac
     5401 tgttttgtcc cagcttctgg ggagggtttc tctaatagct atatttcggt aattactgta
     5461 aatggttcaa atactcaaat tggtagttcc aatccgagaa aggttatcag gacggctatt
     5521 tctatccgtt aaccatagaa aatttatcat tttaggctta taatgggcct gatgagcaaa
     5581 aaagaagc
//
LOCUS       DAOSHV010000034         5212 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_14132_length_5212_cov_8.217181, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000034 DAOSHV010000000
VERSION     DAOSHV010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 5212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5212
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14132_length_5212_cov_8.217181"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2281
                     /locus_tag="PKZ29_01820"
     CDS             <1..2281
                     /locus_tag="PKZ29_01820"
                     /inference="COORDINATES: protein motif:HMM:NF040179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG37639.1"
                     /translation="KDKKTNEPTVGEKLTGTENKVETEGKKPFNEGLVSTKDIIAPAS
                     LEVDFNHVRIDDKYYRTLFVASYPRFVGINWLSSLINFDATLSVSMYIYPTDGKAILD
                     DLRRKITEMEAEISTDIQRGRIVNPATQAKLEDALNLQADLVKGEERFFQFGLYITIS
                     ADTREELDRINKNLEAALGSLLMVSKRATLQMEDGFITGLPAGDDRLNITRNMDTTSL
                     ATTFPFVSSDLSDDKGVMYGINIHNGSLVIFDRFSMQNYNSVVFATAGAGKSYMIKLE
                     VLRSLMLGAEIIVIDPENEYRDLAESVGGQYIAFGYGEESKINPFDLSLTEEEGENAL
                     NSKVLSVHRLMKIMLGNMDPMEESILDRAMMDAYKMKGITPDPTTQDREPPLIEDVYK
                     SLIGMEEEKAKMLAARMEKYIKGSFAGIFNQKTTVNLVNPFVVFGIKNLEDSLRPVAM
                     NIILDYIWTVVKRSLKKRILVVDEAWYLMQYEDSAAFLRGIVKRGRKYYLGVTTITQD
                     VDDFLQTDYGKEIVTNSAIQILLKQHAAAIDQIGEVFYLSEGEKQLLLTADKGQGIFF
                     AGQNHVAIQVISSDEEHKLITSNPEEVLNKKKIVIEKPLGPDLKEENKNIVSTPLPPK
                     PQPVLEKPVTELKQEESEASEIETVLQRKIETLEENENKLLQEKMPEKKLEGEIKISQ
                     SESKPPTRFVPPVRPEIIKPEEKQKPIFIAGQETKVPQPPQLIKKPVPVFEEKKDKNQ
                     TEEEKSGLNYDDLFGNGIV"
     gene            2315..4492
                     /locus_tag="PKZ29_01825"
     CDS             2315..4492
                     /locus_tag="PKZ29_01825"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HOG37640.1"
                     /translation="MAVLESDQNQIKSSKDNKGVVRSEAELVAELTKLKKSGKKWESG
                     SKLDKEISQIVGVESIPSLEVALNVLNQRVEKQKFDYVEEKISKVAEDVNEQLGGNEE
                     SREKIKTEITKIIDQSEFRTQTEEISALTEALPKKEDNKTEAAITAKEIKISLDKWQE
                     DHIEEVRGYRQQLFEQKFEEEVKKTNPEINADETEAVKIKASLIAKVYFDNGGIENQK
                     NEAIEANKGESIGKLRNSWTEVQALVGLMRKSSSEIEETVKKNDEVDSRLARIRIPYE
                     NLPQARAYDDVINNFRQPEFRQTFESISGGVRTGFRMVNEVSRMTGGWMEKGIVKVAN
                     NFAAKIGNQAASGFVKNSVGVLAKEGMSNGLKTVLSGLAQGGVKAVASGAATAGAATG
                     AGIAAGAAAGPPGWIIAAAMLGKKIIGGIADKLGINFVKKLKEGLAVTGNKMIDGVIS
                     GAGSLVAVPTMAAGTTVAAGGIVVAVIVGLFVYQMLQSNNISSLVPKIETEEGGGGGG
                     GRPPVYGEITYTDDGRIRDCGVGMEVDFKRRRNKTRVSPGVLANGAYDEEFEEAVGES
                     FGTRCGVVYAAQYLAFDFKYWVPYYWAGKYPRRGLNPMWGEEIRPDHNGRIYRGLDCS
                     GFVTWAQYSGYGAKTGASTSVAFASGRNCERIKSLIEPGDTITLNDENGYHVAMVVAY
                     DDKYIKFAHSGGGSGVSTGLISLCTGRGVDQGMNFEYLHKKIY"
     gene            4498..4968
                     /locus_tag="PKZ29_01830"
     CDS             4498..4968
                     /locus_tag="PKZ29_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37641.1"
                     /translation="MKIINWLKRNLLLIILVFIVILLSLIKLRANKQVAEITKPVIPT
                     PTEILRPETEVKYNNKTKKEILEMDPEEGYDFITELDKNEVEKLDMMPNYDFSDFLPE
                     RKTTFVAEKYDPVKRELIVQPLITNKTEITDEVKNWLFYETGNNPKTIKIIWNE"
BASE COUNT         1891 a          666 c         1336 g         1319 t
ORIGIN      
        1 aaaagataaa aaaacaaatg aaccgacggt gggagaaaaa ctgacgggga cagagaataa
       61 agtagaaaca gagggaaaaa agccgtttaa tgaaggcttg gtatcaacaa aggatattat
      121 tgcgccggca tcattggagg tagattttaa tcatgtcagg attgatgata aatactaccg
      181 aacgttgttt gtggcatctt atccccggtt tgtggggata aattggctat cgtcactgat
      241 taattttgat gcgactttga gcgtgtcgat gtatatttat ccgactgatg gcaaagccat
      301 tttggacgat ttgaggagaa aaattacaga aatggaggca gaaatcagca ccgatattca
      361 aagaggacgg atagtcaacc cggcaactca ggcaaagttg gaggacgcgc ttaatttgca
      421 ggcagatttg gttaaaggag aggaaaggtt ttttcaattc ggtttgtata taacaatttc
      481 agcagacaca agagaagagt tagacagaat taacaaaaat ttggaggcgg ctttggggtc
      541 gcttctgatg gtgagcaaga gggcgacgtt gcaaatggag gacgggttta ttacgggact
      601 gccggcgggc gatgatcggc tgaatattac cagaaatatg gatacaactt cgctggcaac
      661 aacgtttccg tttgtatcat cggatttgtc agatgataaa ggggtgatgt atgggattaa
      721 tattcacaat ggttcgttgg tgatttttga ccggttttcg atgcaaaatt acaattcggt
      781 ggtatttgct accgccggcg ccggcaaatc atacatgatt aagttggagg tattaagatc
      841 gttgatgctg ggggcggaaa ttattgtaat tgatccggaa aatgaatatc gggacttagc
      901 agaatcggtg ggaggtcaat atattgcctt tggttatggt gaggagtcaa aaataaatcc
      961 gtttgatttg tcactgacag aagaggaggg agaaaatgcc ttaaacagca aggtattgtc
     1021 tgttcatcga ctgatgaaaa ttatgttggg aaacatggat ccgatggagg aatcaatttt
     1081 ggaccgggca atgatggatg cctacaaaat gaaggggata actccagatc caacaactca
     1141 agacagagag ccgcctttga ttgaggatgt ctataaatca ttaatcggga tggaggaaga
     1201 aaaggcaaaa atgctggcgg caagaatgga aaaatatatt aaaggctcgt ttgcgggaat
     1261 ttttaatcaa aaaactacag ttaatttggt taacccattc gtggtgtttg ggattaaaaa
     1321 tctcgaggat agtttacgac cagtagcgat gaatataatt ttggattata tttggacggt
     1381 agttaagaga agcttaaaaa aaaggatttt agtggtggat gaagcgtggt atttgatgca
     1441 atatgaagat tcagcggcat ttttgagagg aattgtaaaa cggggacgaa aatattattt
     1501 aggggtgacg acaattactc aggatgtaga tgattttttg caaactgatt atggtaaaga
     1561 aattgtgact aacagcgcca ttcaaatttt gttgaaacag cacgcagcag caatcgacca
     1621 gattggtgaa gtattttatc taagtgaggg agaaaaacag ttattgttaa cggctgataa
     1681 gggtcagggg atattttttg cgggacaaaa tcatgtggca attcaggtaa tttcttcaga
     1741 tgaggagcat aaattaatta cctccaatcc cgaagaggtg ctaaataaga aaaaaatagt
     1801 aatagaaaag ccgctgggac cggacttgaa agaggaaaat aaaaacatag tttcaactcc
     1861 cttgccacct aaaccccaac cggtattaga aaaaccggtg acagagttga aacaagaaga
     1921 gtctgaagcg tcggaaatag aaacggtatt gcaaagaaaa attgaaactt tggaggaaaa
     1981 tgaaaataaa ttgttacagg aaaaaatgcc ggaaaaaaag ttggaaggag agataaaaat
     2041 tagtcagtca gaatctaaac caccaacaag atttgtacca ccagtaagac cggagataat
     2101 taaaccagaa gagaaacaaa aaccgatatt tatagctggt caggaaacga aagtgccgca
     2161 gccaccgcaa ttaattaaaa aaccagtgcc ggtgtttgaa gaaaaaaaag ataaaaatca
     2221 aacagaggaa gaaaaaagcg ggttgaatta cgatgatttg tttggcaacg ggatagtata
     2281 atcttgaggg taaaaattgg tataattttt ggtaatggca gttttggaat cagatcaaaa
     2341 tcaaataaaa tcgtctaaag acaacaaagg ggtagtgagg tcggaggcgg agttagtggc
     2401 cgaattaact aagcttaaaa agagtggcaa aaaatgggaa agtggaagta aattagataa
     2461 ggaaataagc caaatagtcg gagttgagtc aattccgagt cttgaggtgg cattaaatgt
     2521 gttaaatcaa cgggtggaaa agcaaaaatt tgactatgtt gaagaaaaaa ttagtaaagt
     2581 ggctgaggat gttaatgaac aactgggagg aaatgaggag tcaagagaaa agattaaaac
     2641 agaaataact aagataattg atcaaagtga atttagaacc caaaccgagg aaataagcgc
     2701 tttaactgag gctttgccca aaaaagaaga taataaaaca gaagcggcaa tcacagccaa
     2761 ggaaattaaa atatctttgg ataaatggca agaagatcat attgaagagg ttagaggata
     2821 tagacaacaa ttatttgaac aaaaatttga agaggaagta aaaaaaacaa acccagaaat
     2881 aaatgcggac gagaccgagg cagtaaagat aaaagctagc ttaatagcca aggtttactt
     2941 tgacaacggg ggaattgaaa accaaaaaaa tgaagctata gaagcaaata agggtgaatc
     3001 aataggaaaa ttgagaaact catggactga ggttcaggca ttagtaggtt taatgaggaa
     3061 gagttcgtca gaaatagaag agactgttaa gaaaaatgat gaggttgact caagactagc
     3121 cagaatcaga ataccttatg aaaacttacc ccaagcaagg gcttatgacg atgtaattaa
     3181 caattttaga caaccagaat ttagacaaac atttgaatca attagtggcg gggttagaac
     3241 tggatttagg atggttaatg aagtcagccg aatgaccgga ggctggatgg agaaagggat
     3301 agtaaaagta gcgaataatt ttgctgcaaa aatcggcaat caggcagcat ctgggtttgt
     3361 aaaaaattca gtaggagtgt tggcaaagga aggaatgagc aatggactga aaacggtttt
     3421 aagcggattg gctcaaggag gggttaaggc ggtggcgagt ggagcggcaa cggcaggagc
     3481 ggcaactgga gcaggaatag cagcgggagc ggcggcggga ccgccgggat ggataatagc
     3541 agccgccatg ttgggcaaaa aaataattgg gggtatagcc gataagttgg gaattaattt
     3601 tgtgaagaaa ctaaaagaag gtttggcggt aaccggtaat aagatgattg atggagtgat
     3661 ttccggagcg gggtcattgg tggctgtgcc gacaatggca gcagggacaa cagtggcggc
     3721 gggaggaata gtggtggcag taattgtggg tttgtttgtt tatcaaatgc tgcaatcgaa
     3781 taatatttcc agtttagtgc caaaaataga gacagaagaa ggtggaggag gtgggggggg
     3841 acggccacca gtttatggag aaattaccta cacagatgat gggagaatta gagattgtgg
     3901 agtaggtatg gaggtagatt ttaaaagaag gagaaataaa acaagagtaa gcccaggagt
     3961 attagcaaat ggagcctatg atgaagaatt tgaggaagct gttggcgaat cttttggaac
     4021 gagatgtgga gtggtttatg cagctcaata tttagctttt gattttaaat attgggtacc
     4081 ttattattgg gcgggaaaat atccgagaag agggttaaat cctatgtggg gagaggaaat
     4141 tcgtcctgat cataatggtc gaatttatcg aggtcttgat tgtagtggtt ttgttacctg
     4201 ggcacaatat agtggatatg gtgcaaaaac tggtgcttct acttccgttg cgtttgctag
     4261 tggtagaaat tgtgaaagga ttaaatcatt aatagaacca ggagatacaa ttacattaaa
     4321 tgatgaaaat ggctatcacg tggcgatggt ggtggcatat gatgataaat atataaaatt
     4381 tgctcattca ggaggaggta gtggagttag tacaggttta attagtctgt gcacagggag
     4441 aggtgttgat cagggaatga attttgaata tttacataaa aagatttatt aaaaaatatg
     4501 aaaataatta attggttaaa aagaaattta ttattaataa tattggtttt tatagtaatt
     4561 ttattatcgt taataaaatt aagggcaaat aagcaagttg cagaaataac aaagccggtc
     4621 ataccaactc ctacagaaat actaagacca gagactgaag tcaaatataa taataaaact
     4681 aagaaagaaa ttttagaaat ggatccggag gaaggttatg attttataac tgaattagat
     4741 aaaaatgaag ttgaaaaatt agacatgatg ccaaattatg atttttctga ttttttgccg
     4801 gagagaaaaa caacatttgt ggcagaaaaa tatgatccag ttaaaagaga gttgatagtt
     4861 caacccttaa taaccaataa gacagaaatt actgatgagg tgaaaaattg gctgttttat
     4921 gaaactggaa ataatccaaa aacaataaaa attatttgga atgagtgaaa tttgtcaaaa
     4981 aatacgcttt aatcatggtt ttgagcgcag taacagtatc aatgatgatt tacaaactgt
     5041 ggccgactaa ttagtcttgg agacggacgg gcatgatgat gtgagtaaaa gaggggtctg
     5101 attggtcatg aaaaaagacc ggggtgagac tttcgttgag ttcaattaga atttcttggc
     5161 ttttgcaaat attaagaaaa tcacttaaaa atttgtaatt gaaagcaact tc
//
LOCUS       DAOSHV010000035         5090 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_14595_length_5090_cov_5.856207, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000035 DAOSHV010000000
VERSION     DAOSHV010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 5090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5090
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14595_length_5090_cov_5.856207"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            206..1117
                     /gene="era"
                     /locus_tag="PKZ29_01835"
     CDS             206..1117
                     /gene="era"
                     /locus_tag="PKZ29_01835"
                     /inference="COORDINATES: protein motif:HMM:NF000908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOG37642.1"
                     /translation="MENKSKNQIKIGKVVLIGRPNTGKSSLLNAIMSQKVAITSPLPQ
                     TTRKNSRVVYSDKKGKIIFSDTPGIMAKIDDLVGKRINKEAPKELNQADVILAVVDIS
                     RPKNEEENKVIGLLRKVAVKKILVYNKIDIARGPKDHLAEYNYLEDEFDRTIEVSATK
                     EKNIKGLINLIFELLPAKANQETKYNIQLIEEKNKPKIGINSREYIAEIIREKAYLFL
                     RKEVPYSIDVEVESVVDKRKLILIKATILTTAERYKKMIIGQGGKKIKEIGYNARKEL
                     ELMSERKVYLELRVEVDKHWPEREINK"
     gene            1184..1618
                     /locus_tag="PKZ29_01840"
     CDS             1184..1618
                     /locus_tag="PKZ29_01840"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HOG37643.1"
                     /translation="MTQKSNGGKFFLTGLLAAAAGAIGGLLFAPKSGKETREDLRKLA
                     VKLNKEVKTNVTDTKTKVKEVFGEATEETVKKYNEIKSAVMGKIAEVKTAGKEIDKGK
                     YALIVEDVVSEFKGDLRATKDGMTKLAAQLKKDWEKVKKALA"
     gene            complement(1610..2563)
                     /gene="obgE"
                     /locus_tag="PKZ29_01845"
     CDS             complement(1610..2563)
                     /gene="obgE"
                     /locus_tag="PKZ29_01845"
                     /inference="COORDINATES: protein motif:HMM:TIGR02729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOG37644.1"
                     /translation="MALIDEVRITIKGGDGGNGVVHFYTDASQTKGGPDGGNGGDGGS
                     VYFEGVSDITKLNQFSHSKVYSAQNGQPGGRNKRSGKKGDDLIISVPVGTVANYDNGS
                     SVEVVEVGQKILMAKGGSGGRGNFVFRSATNQTPQQSEPGFKCQPKKLFLQLKLIAQV
                     GLIGLPNAGKTSLLNELTRAKAKVANYAFTTLEPNLGISRSGHIIADIPGLIEGAAAG
                     RGLGIKFLKHIERTKLLLHCVSAESADIIKDYHTVRTELANYSSALAQKPEIIILTKS
                     DTVDPDRLTSLQNKIGADLVTSIIDTDSIKHLSDFIAKKLS"
     gene            complement(2609..2920)
                     /gene="trxA"
                     /locus_tag="PKZ29_01850"
     CDS             complement(2609..2920)
                     /gene="trxA"
                     /locus_tag="PKZ29_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019039134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOG37645.1"
                     /translation="MAKQIQQTDFQSEVIDHKGKVLVDFWAPWCGPCQMLGPIIEEIS
                     QEVKDAKVVKLNVDESQEVAAKYNIISIPTVILFKDGQIVDTLIGFRQKQEYLDAINK
                     A"
     gene            complement(2938..3336)
                     /locus_tag="PKZ29_01855"
     CDS             complement(2938..3336)
                     /locus_tag="PKZ29_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37646.1"
                     /translation="MSDENQSQTLINIESLINTASSRMDELSRLLKEQKSMIDDLLSN
                     DNQYQTIEQEVKKQTKVKASSRQNVLNSPEAKPVLEKIKDNQQQLKELKIALSDYLSQ
                     YVKLSGTNQIEGPDGVVRQIIYTAKLVKSK"
     gene            complement(3350..4396)
                     /locus_tag="PKZ29_01860"
     CDS             complement(3350..4396)
                     /locus_tag="PKZ29_01860"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HOG37647.1"
                     /translation="MNFLTKSQPIIGLAPMDGVTDEAFRLVQTQIAKPDLIFTEFVSA
                     EGISRGGVKLYDTLLFSHHERPIIGQLFGKDPDSFYKSAVILSQLGFDGIDINMGCPA
                     KTVTQHGSGAALISKPDLAVEIIQSVKRGISDWFTGKTKITDLKLNQKTLEVIGRNQT
                     YSQTNRHIKKTNLPPTISVKTRLGIDQSIIETWAEILLNQNLDFLTIHGRTLKQAYSG
                     QANWSEIKKVVDLAKNTKTKVLGNGDITSRCQGLEYCQKYGVDGVLIGRAAGGNPWCF
                     SNHLATPQEKFSAMLLHAQIFSEVFPQRCFDSLRKHFLLYTADFPHAKSLRMKLVRLN
                     SVKELLSLESEFQS"
     gene            complement(4393..5052)
                     /locus_tag="PKZ29_01865"
     CDS             complement(4393..5052)
                     /locus_tag="PKZ29_01865"
                     /inference="COORDINATES: protein motif:HMM:NF012305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HOG37648.1"
                     /translation="MNQTKPVSKRLFIGSLPYRFTEGELLNLFISEGKIVSIRIMHNQ
                     WGRSRGLGYVEYENLDDAISAKQKYHNYKIADRTIIVDYAQPDPFLTPEGQARHQEAL
                     KNHPRRSQKPAYFDSPDSPNRQQSDFHSQPKRKYKGGKPVFNSLGKSLNRNTTGEYIP
                     KFFPDSSAKKVKKYRPKPGQKPEYKSSHPAHVRSSVFKQRNFGAKSGAKFAARSRKKS
                     S"
BASE COUNT         1604 a          855 c         1167 g         1464 t
ORIGIN      
        1 aattcattga ttctttgcga ccaaaaaaaa gcgaagtttc gactgaagga aaaagaacaa
       61 tatttctgag aggtataagc ggcagcactt tgcgaatagg ctgaactgca ctggtttgac
      121 ttgattctga tggcatattt agcagtctat cattttgatt gctaagtgtc aatcaataaa
      181 atagaactta gtgtaaaata cacttgtgga aaataaaagc aaaaaccaaa taaagatcgg
      241 caaagtagtg ctgataggca gaccaaatac gggcaaatcg agtttgctaa acgcgattat
      301 gtctcaaaaa gtggcgataa cttcacctct acctcaaact accagaaaaa acagccgggt
      361 agtttacagc gacaaaaaag gcaaaataat tttttctgat actccgggaa ttatggctaa
      421 aattgatgat ttggtgggaa aaaggattaa caaggaggcg ccaaaagagc taaatcaggc
      481 agatgtgatt ttggcggtag tggatatatc ccgaccaaaa aatgaagagg aaaataaagt
      541 tatcggtctg ttgagaaagg tagccgtaaa aaaaatactg gtttataaca aaatagatat
      601 cgccagagga cccaaggatc atttggcgga atataattat ctggaagacg agtttgaccg
      661 gacaattgaa gtaagcgcca ccaaagaaaa aaatattaaa ggtttgatta atcttatttt
      721 tgaattgctg ccggcaaaag ctaatcagga aacaaaatat aatattcaac tgatagaaga
      781 aaaaaataag cctaaaatag gtataaattc gagagaatat attgccgaaa taatcagaga
      841 aaaggcttat ttatttttga gaaaggaagt cccctactcg atagacgtgg aggtagaaag
      901 tgtggtagac aaaagaaaat tgattttgat aaaagcgact attttgacca cggcagaaag
      961 gtataaaaaa atgataatcg gtcagggggg gaagaaaata aaagaaatcg gttataacgc
     1021 cagaaaggaa ttggagttga tgtcggagag aaaagtatat ttggagttga gagtggaagt
     1081 agataaacac tggccagaaa gagaaataaa taagtaaaat ttgttgacag gcagggaaat
     1141 ttactttaga ctgaattgtt atttaattta tttttgacaa aatatgacac aaaaatctaa
     1201 tggaggaaaa ttttttctaa caggtttgtt ggctgcagca gccggagcta tcggtggctt
     1261 actttttgcc cctaagtctg gcaaagaaac cagagaagac ttaagaaaat tagccgttaa
     1321 actcaacaaa gaggttaaaa ccaatgtcac tgataccaaa actaaagtaa aggaagtttt
     1381 tggtgaggca acggaagaaa cggttaaaaa atataatgaa attaaatcag ctgtgatggg
     1441 taaaattgcg gaagtaaaaa cagcaggaaa agaaatagac aaaggtaaat atgccttgat
     1501 tgtggaggat gtggtttctg aatttaaggg agatttgaga gccaccaaag acggcatgac
     1561 caaactggca gctcaactca aaaaagattg ggagaaagtc aaaaaagctt tagcttaatt
     1621 ttttggcaat aaaatcactg aggtgtttga tactatcggt gtcgataatg gaagtaacca
     1681 ggtcggcgcc gattttgttt tgaagggatg taagacggtc ggggtcaaca gtatcggatt
     1741 tggtcagaat aataatttct ggtttttggg ctaaagcgga gctataatta gccagttcgg
     1801 tacggacagt gtgataatct ttgataatat cggctgattc ggcggaaaca caatgaagca
     1861 aaagtttggt tctttcgatg tgtttgagaa attttatgcc taatccccga ccggcagcag
     1921 cgccttcaat tagtccggga atatcggcga taatatgacc ggaacgactg atgccaagat
     1981 ttggttccag agtggtaaaa gcataattgg caaccttagc cttggcgcgg gtaagttcgt
     2041 taagaaggct agttttgccg gcattgggca aaccaatcag cccgacttgg gcaattaact
     2101 ttagttgaag aaaaagtttt ttgggttggc acttaaatcc tggttctgac tgctggggag
     2161 tttgattggt ggcggatcta aaaacaaagt ttccccggcc gccactaccg cccttagcca
     2221 taagaatttt ttgaccaact tcaactactt ctacagagga accgttgtca taattagcaa
     2281 cagtgccgac gggaacagaa ataatcaaat catcgccttt tttaccgctt cgtttgttgc
     2341 gaccaccggg ctgaccgttt tgagcgctgt aaaccttaga atggctgaat tggtttagtt
     2401 tagtgatatc agaaacaccc tcaaaataaa ctgaaccacc gtcgccgccg ttgccaccgt
     2461 cagggccgcc tttggtttgg gaggcgtcgg tataaaaatg aactacacca ttgccaccgt
     2521 cgccaccttt gatagtaata cgcacttcgt caattaaagc catgagagta ttataaacta
     2581 atctaccgat aaaactaagc tttgcaattt aagctttgtt gatggcgtca agatattcct
     2641 gtttttgtct aaaaccaatg agagtgtcga ctatctgacc gtctttaaaa agaatgacgg
     2701 taggaatgct gatgatgttg tatttggcgg caacttcctg agattcatcg acattaagtt
     2761 taacaacttt ggcgtctttg acctcttgag aaatttcttc gataatcggg ccaagcatct
     2821 ggcaaggacc gcaccaaggg gcccaaaagt cgacaagaac cttgcctttg tgatcaataa
     2881 cttctgactg aaaatcggtt tgttggattt gttttgccat aggtgaatta taaactatta
     2941 ttttgatttg actagtttgg cagtgtaaat aatttggcga acaacaccgt cggggccttc
     3001 aatttggttg gtgcccgaaa gtttaacata ttgcgataaa tagtctgata aggcgatttt
     3061 aagctctttt agctgctgtt gattatcttt gattttttct aataccggct tggcttcagg
     3121 agaattgagg acattttggc gggaggaagc cttgacttta gtttgttttt taacttcctg
     3181 ctcaatggtt tgatattggt tgtcgtttga taaaagatcg tcaatcatac ttttttgttc
     3241 ttttagaaga cgactcaact catccatccg gctactggca gtattgataa gagattcaat
     3301 attaataagg gtttgagatt gattttcgtc cgacatattc tctactctat caactttgaa
     3361 attctgattc aagggataat aattctttga cagagtttaa tctaacaagt ttcattctga
     3421 gtgatttagc atgaggaaaa tcagcagtat aaagcaaaaa atgtttgcgg agactatcaa
     3481 aacaacgctg gggaaaaact tcagaaaaga tttgggcgtg gagcagcatg gcggaaaatt
     3541 tttcctgagg ggtggctaaa tgattggaaa aacaccaggg gttgccgccg gcagcccgac
     3601 caattaaaac accatcaaca ccatattttt ggcagtactc tagcccttgg cagcgggagg
     3661 taatatcgcc attgcccaaa accttagttt tggtattttt ggccaggtca actacttttt
     3721 tgatttcaga ccagttggcc tgtccagaat aggcttgttt tagagtgcga ccatgaatgg
     3781 ttaaaaaatc taaattttgg tttagtaaaa tttcggccca agtttcaatg atagattgat
     3841 caatccctaa gcgggttttg acagaaattg tgggcggtaa attggttttt ttgatatgac
     3901 ggttagtttg ggaatatgtt tggttacgac caataacctc taaggttttt tggttgagtt
     3961 tgaggtcagt aattttggtt ttgccggtaa accaatcact aatcccccgc ttgactgatt
     4021 gaataatttc aacggctaaa tcgggtttac taattagagc ggcgccgcta ccgtgttggg
     4081 tgacagtttt ggcggggcaa cccatattaa tatcaatacc gtcaaatcca agttgggaca
     4141 aaattacagc tgatttataa aaactatcgg gatctttgcc aaataattgc ccaataatag
     4201 gtctttcgtg gtgagaaaac aaaagagtgt cataaagttt gactccacca cgactaatac
     4261 cttcagcgct gacaaattcg gtaaagatta aatcaggttt ggcaatctga gtttgaacca
     4321 accgaaaagc ctcgtcggta actccatcca tgggggcaag accaataata ggctgagact
     4381 tggttaaaaa attcatgacg atttttttcg gcttctagcg gcgaatttag ctccggactt
     4441 ggcaccaaaa tttctttgtt taaagacact ggaacggaca tgagcggggt ggctggattt
     4501 atattcaggt ttttgacccg gtttgggtcg gtattttttg acttttttgg cgctagagtc
     4561 gggaaaaaat ttgggaatgt attcaccggt agtatttcga ttaagtgatt ttcccagtga
     4621 attgaacact ggtttgccgc ctttatattt tcgttttggt tgagaatgaa aatcggattg
     4681 ctgacggttg ggagaatcgg gagagtcaaa ataagctggt ttttggctac ggcggggatg
     4741 atttttgaga gcttcttggt gacgggcttg accttcgggg gttaaaaagg gatcgggctg
     4801 ggcatagtca acaataatgg ttcggtcagc aattttgtag ttatgatatt tttgtttggc
     4861 gctaatagca tcgtccaaat tttcatattc gacataaccc aagccccgac ttctgcccca
     4921 ctgattgtgc ataattctga tagaaacaat tttcccttca gaaataaata aatttaaaag
     4981 ctccccttcg gtaaagcgat aaggcaaact gccgataaat agacgcttag agacaggttt
     5041 ggtttggttc ataaatatga gttattatac tatgatgaag tatgaagtat
//
LOCUS       DAOSHV010000036         5083 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_14618_length_5083_cov_4.471559, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000036 DAOSHV010000000
VERSION     DAOSHV010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 5083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5083
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14618_length_5083_cov_4.471559"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1236
                     /locus_tag="PKZ29_01870"
     CDS             <1..1236
                     /locus_tag="PKZ29_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37649.1"
                     /translation="RFGVTGSGFIWYGLNQPVLDRAFDAWNLKNEQYYWQLTTALQKN
                     DPDQLNQLFNKYAIEYVIYDNNVYFPDDQIYNQVAINTKETLFDMSQRQHLTQVAQFG
                     QITVYQTAYPTKPFVVNHPVSVYPFDFSYSDPAFNQHQHYLNSSPINLDYPFVDLFTN
                     RLPSHQNFSVLSDDQSLTLISRQHSYTFAKDQAANLKFNFSLPNQNSPLLVYNFPQAQ
                     LNLDYLVEVNYRHLSGLPVQITAVSENTRHKYFDTKLTKSLTDTTSWFIIPAHQDEDF
                     QPGINLILNPSPIKEFPSKNQINYVKIYPFNLESLINQSVTTNPDITPNSYLIYPQSY
                     SPHWLAFYFQGLKPVFLTHHSLADNWANAWQLPPTIYPSRIHFFFWPQIFQHLGLVIT
                     ITTLILSLKSKPKLHSLKS"
     gene            complement(1247..2401)
                     /gene="glyA"
                     /locus_tag="PKZ29_01875"
     CDS             complement(1247..2401)
                     /gene="glyA"
                     /locus_tag="PKZ29_01875"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF000586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOG37650.1"
                     /translation="MDKQIQNLIKKECQRQEKMVHLIPSENFVSKEILAALGSCLTNK
                     YAEGYPGRRYYQGNEFIDKIENLAIERGKKLLGTPHMNVQPYSGSPANSAIQMALLER
                     GDKICGLKLSAGGHLTHGHPKITFSGKFFESVQYDVEADGRIDYDKLEKLVMAEKPKM
                     IMAGTTAYPYILDFKRFGKIADKAGAWLVADVSHIIGLIVAGVHPSPVPYADVIMSTT
                     HKSLRGPRGAFIAVTQKGLKKDEMLATKIDKAVFPGMQGGPHENQIAALAVAFREAGS
                     VGFKKYAAQVVKNAKVLAETLRKNGIKVYGTENHLMVVEVGLEKGKEAAVELEKQGIV
                     VNANTVPHDQAGPFRPSGIRIGTPAMTTRGWKEKEFRKLGEKMAGIIKRI"
     gene            complement(2416..3375)
                     /locus_tag="PKZ29_01880"
     CDS             complement(2416..3375)
                     /locus_tag="PKZ29_01880"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HOG37651.1"
                     /translation="MNKIRLIAGTSHPELAKKIAKNLKIELTPVTIKKFANGEIYARI
                     ESKVRGEDVFVLQTMTEPVNDFVMETLILMDALRRSSAKRINLICPQLAYSRQDRRAR
                     SHEPITAKLLANMLTVAGLSRLITIDLHADQIQGFYDIPVDHLVGYPLIAKEVLAEKY
                     KDIVVVSPDIGGVKRANKLADLLGTTIAIIDKIRREHNKSEVAHVVGDVKGKTAVLID
                     DIIDTGGSICQAANTVKEFGAKEVIICATHALLSGEASKNLDNCATSKVILLDSVPIA
                     KEKRIKKMKIVSLAPLLSQIIYRVHKEKSLGELFRWEKQARLL"
     gene            complement(3372..3977)
                     /gene="tmk"
                     /locus_tag="PKZ29_01885"
     CDS             complement(3372..3977)
                     /gene="tmk"
                     /locus_tag="PKZ29_01885"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:NF014299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOG37652.1"
                     /translation="MKPGKFIVFEGIDGSGKGTQILLLADYLKKNKIGAVLTKEHTRD
                     LAVGKLIEKTVNREEKMNPLALQLCFTADRVDHFEKVIGPAIDEGKIVISDRYYLSTV
                     AYTDRDKKELMMKLNESVVPKADLTFFIDVSPEVAIERLDKSREEKTIFEKYEKLQQS
                     RKNYWWAMRKMKGKVVVMDGSEKPEIIHENILKILRRRKII"
     gene            complement(3974..4699)
                     /gene="thyX"
                     /locus_tag="PKZ29_01890"
     CDS             complement(3974..4699)
                     /gene="thyX"
                     /locus_tag="PKZ29_01890"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007957334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HOG37653.1"
                     /translation="MARETKLKVTLLSFTPDPERNVVAAIRQCYSAVGAKEIKQKTDK
                     ETRERLIRQVMASGHTSTLEHASFTFAIEGVSRVTEIQLIRHRIGCSYSIQSGRYVKR
                     GEAEYVIPPAIVNGDKKVLKKVRQHFKNTQKLYNELIDLGVKAEDARYCQPQSLKTKI
                     VVTMNVRALLHFFNLRCCRRAQWEIQAVANKMLQLVKEKAPVIFENAGASCVSEGICW
                     EGDMSCGLWKSIPGAMLKSRIKK"
     gene            complement(4818..>5083)
                     /locus_tag="PKZ29_01895"
     CDS             complement(4818..>5083)
                     /locus_tag="PKZ29_01895"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HOG37654.1"
                     /translation="NFSFDDVSETQKGLYEAAMKDAKDKAEMLVKVSGRRLGKVVNVS
                     ENGSSSVYPMYRMAAEGLGGGGAPVEPGSGTVRQSLVVVFELK"
BASE COUNT         1286 a         1483 c          665 g         1649 t
ORIGIN      
        1 cgctttggcg tcaccggctc tggttttatt tggtatggtc tcaaccaacc ggttttagac
       61 cgggcttttg atgcttggaa tctcaaaaac gaacaatatt actggcagct gaccaccgct
      121 ctccaaaaaa atgatcccga ccagctcaat cagcttttta ataaatatgc tatcgaatat
      181 gtcatctacg acaacaatgt ttacttcccc gacgaccaaa tttataacca agttgctatc
      241 aataccaaag aaactctctt tgacatgtca caacgtcaac atctgactca agtcgcccaa
      301 tttggccaaa tcaccgtcta ccaaaccgcc taccccacca aaccttttgt tgtcaatcat
      361 cccgtctccg tttatccttt tgacttctcc tattccgatc ccgcctttaa ccagcaccag
      421 cactatctca actcctctcc catcaatctt gattatcctt ttgtagatct ctttaccaac
      481 cgtctgcctt cacaccaaaa tttttctgtc ctttctgacg atcaatctct tactcttatt
      541 tcccgccaac attcttatac ctttgccaaa gaccaagccg ccaatcttaa atttaacttt
      601 tctctcccca accaaaattc ccctctccta gtctacaatt ttccccaagc ccaactcaat
      661 ctggattatt tggttgaggt caactatcgt catctctccg gtctccctgt ccaaattact
      721 gccgtttccg aaaacacccg ccacaaatat tttgatacca aacttaccaa atctcttacc
      781 gataccacca gctggtttat catccccgcc caccaggacg aagattttca acccggtatc
      841 aatcttattc ttaatccttc acctatcaaa gaatttccca gtaaaaatca aattaactat
      901 gtcaaaatat atccttttaa tcttgaatct cttatcaacc aatccgtcac caccaatccc
      961 gatattactc ccaactcata cctaatctat ccccaatctt actctcctca ttggctggct
     1021 ttctattttc agggtctcaa accggttttt ctcacccacc actccctcgc cgacaactgg
     1081 gccaatgctt ggcagctgcc tccaaccatt tatcccagcc gcattcattt tttcttttgg
     1141 cctcaaatct tccaacatct cggtcttgtt attactatca ctactctaat tttaagtctc
     1201 aaatcaaaac ccaagcttca ttctcttaaa tcctaaatca agaatcttaa atccttttaa
     1261 ttatccccgc cattttctct cccagcttcc taaattcttt ttctttccac cctcttgtcg
     1321 tcattgccgg tgtcccaatt ctaattcccg acggtctaaa cggcccggct tggtcatggg
     1381 gaaccgtatt agcatttacc actattccct gtttttctag ctccactgcc gcttccttgc
     1441 ctttttccaa tcccacttct accaccatca aatgattctc tgttccataa acctttatcc
     1501 cattttttct caacgtttct gccaacactt tggcattttt aaccacttga gccgcatatt
     1561 ttttaaaccc aacactcccc gcttctctaa atgccactgc cagcgccgca atttgatttt
     1621 cgtgcggtcc gccctgcatt cccggaaaca ccgccttgtc aatttttgtt gccaacatct
     1681 catctttttt taaccctttt tgggttacag caataaatgc ccctctcgga ccccgcaaac
     1741 ttttgtgagt tgtgctcata attacatccg catatggcac cggactggga tggactcctg
     1801 ccacaatcag tccaataata tgtgacacat cagccaccag ccacgccccc gccttgtctg
     1861 ctatcttgcc aaatctttta aaatctaaaa tataaggata agccgtcgtc cccgccataa
     1921 tcatttttgg cttctccgcc atcactaatt tctccaattt atcgtaatca atccgcccgt
     1981 ccgcttctac atcatattgc acactctcaa aaaatttgcc cgaaaacgta attttgggat
     2041 gaccatgggt caaatgaccc ccggccgaca attttaaccc acaaatttta tccccccgct
     2101 ccagcaaagc catttgaatc gcgctgttgg ccggacttcc cgaatacggc tgcacattca
     2161 tatggggcgt ccccaacaat tttttacccc tttcaatcgc caaattttct atcttatcaa
     2221 taaattcatt cccttgataa taccgccttc ccggataacc ctcggcatat ttgtttgtca
     2281 gacaacttcc caatgctgct aatatttcct ttgatacaaa attttcactc ggaatcaggt
     2341 gaaccatttt ttcctgcctt tgacattctt ttttgattaa attttgtatt tgcttgtcca
     2401 tttttaaatt taaaattata acaatctcgc ctgcttttcc cacctaaaca attctcccaa
     2461 cgatttttcc ttgtgcaccc gataaataat ttgactcaac agcggcgcca acgatactat
     2521 tttcattttt ttgattcttt tttcttttgc tatcggcacc gagtccaaca aaataacttt
     2581 tgacgtcgcg caattatcca aatttttgct tgcctccccg cttagcaacg catgggtagc
     2641 acaaattatc acttccttgg ccccaaactc tttaaccgta tttgctgcct gacaaattga
     2701 tcctccggta tcaataatat cgtcaattaa cactgccgtt ttgcctttaa catcaccgac
     2761 cacatgagcc acctctgact tattatgctc ccgcctgatt ttgtcaataa tggcaattgt
     2821 tgttcccaac aaatccgcca atttattagc tctcttcacc ccgccgatat ccggcgatac
     2881 cactacaata tccttatatt tttctgccaa aacctccttg gctatcaacg gataccccac
     2941 caaatgatcc actggaatat cataaaaccc ctgaatttga tccgcgtgca gatcaatcgt
     3001 aatcagccgg ctcaaacccg ctaccgtcaa catgtttgct aacaatttgg ctgttatcgg
     3061 ctcatgggac ctggctctcc tatcctgccg ggaataagcc aactgaggac agatcaaatt
     3121 aattctcttg gccgatgacc gtcttagggc atccatcaaa atcaatgtct ccatcacaaa
     3181 atcattcacc ggctcggtca tggtttgcaa cacaaacaca tcctcccctc tcaccttact
     3241 ctctatccgg gcataaattt ccccattggc aaactttttg attgttaccg gggtcagctc
     3301 aatttttaaa tttttggcga ttttttttgc caattccgga tgggaagtcc ccgcaattaa
     3361 tctgatttta ttcatataat tttcctcctc ctaagtattt ttaatatatt ttcatgaatt
     3421 atttccggtt tttcgctccc atccatcacc accacctttc ctttcatctt cctcatcgcc
     3481 caccaataat ttttccttga ttgttgcaat ttttcatatt tttcaaaaat agttttttcc
     3541 tcccggcttt tgtccaacct ttcaattgcc acttccggcg acacatcgat aaaaaaagtt
     3601 aaatcagctt ttggcaccac tgactcgttt agtttcatca tcaattcttt tttgtccctg
     3661 tcggtatatg ccactgtcga caaataatac ctgtcagaaa taactatttt cccctcgtct
     3721 atcgccggtc cgatgacttt ttcaaaatgg tccactctgt cggctgtaaa acataactgc
     3781 aatgccaacg gattcatttt ttcctccctg ttaactgtct tttcgatcaa cttccccacc
     3841 gccagatccc gggtatgttc cttggtcagc accgccccga ttttgttttt tttcaaataa
     3901 tccgctagca acaaaatctg ggttcccttt cccgacccat caattccttc aaatacaata
     3961 aatttacctg gtttcatttt tttattcgac tttttaacat ggctccggga attgatttcc
     4021 acagcccgca gctcatatct ccctcccagc aaatcccctc agacacacaa cttgctcccg
     4081 cattttcaaa aataaccggc gccttttctt taactaattg cagcattttg tttgccactg
     4141 cctgaatttc ccactgagcc cgtcgacaac accgcaaatt aaaaaaatgt agcaaagctc
     4201 taacattcat tgtcaccact attttagttt tcaaagactg gggctgacaa tatcgagcat
     4261 cctccgcttt tacccccaaa tcaatcaatt cattataaag tttttgcgta tttttaaaat
     4321 gctgtctcac tttctttaaa acctttttat ctccgtttac tattgccggc ggaattacat
     4381 actctgcctc gcctctcttg acatatctcc ccgactgaat cgaataagaa cagccaattc
     4441 tatgtcggat taattgaatc tcagtcaccc gacttactcc ttcaattgca aaagtaaaac
     4501 tggcatgttc caatgtcgaa gtatgtcctg atgccatcac ctgtctaatc aatctttctc
     4561 tggtttcctt atcagttttt tgtttaatct ctttggctcc caccgcgcta taacactgtc
     4621 tgattgccgc caccacattc ctctctgggt cgggggtaaa acttaacaaa gtaactttta
     4681 attttgtctc tcttgccatc ttaaaaaata ttaattttta aaccctgacc caatttagct
     4741 tcttgtcact ccaaaatcaa tcgactaaaa ctaggtcaaa aaaaatggca ggattttttt
     4801 aactatctta ttttaattta ttttaattca aacactacca ccaaagactg tctcactgtc
     4861 cccgatcccg gctctaccgg cgctcccccg ccgcccaaac cctctgccgc catccgatac
     4921 atcggataga ccgagctgct gccattttca gacacattta ccaccttgcc taatctcctt
     4981 ccagacactt ttaccaacat ttctgctttg tctttggcgt ccttcattgc cgcttcatac
     5041 aaaccttttt gggtttcact cacatcatca aaagaaaaat tcg
//
LOCUS       DAOSHV010000037         4984 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_15016_length_4984_cov_3.537026, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000037 DAOSHV010000000
VERSION     DAOSHV010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 4984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4984
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15016_length_4984_cov_3.537026"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="PKZ29_01900"
     CDS             complement(<1..211)
                     /locus_tag="PKZ29_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37655.1"
                     /translation="MKKITALLYIVLSISFLGLVMKVEARKSERLITSTEVMVKSLSL
                     SPMPTGMVATETAEVIEVVPTLASVV"
     gene            353..691
                     /locus_tag="PKZ29_01905"
     CDS             353..691
                     /locus_tag="PKZ29_01905"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37656.1"
                     /translation="MTSSLLAQVNIGNNLLDNSGTAVGPKYNTASPLINSLLKNSLTL
                     AGIIFLCLLIFGGISMIINAGSADSKKTAQAQQAISSALIGFAVVFSAYFIIQIISLV
                     TGVKILDSGL"
     gene            698..1018
                     /locus_tag="PKZ29_01910"
     CDS             698..1018
                     /locus_tag="PKZ29_01910"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37657.1"
                     /translation="MLAIFGSISNPTNYNSQGGSGLFLLLSNIFKLAGVIAGLFFVAQ
                     LIIAGYGYLSSSGDPKKTEAARNKITQSLIGLIIVAGAFILTSVVGSILNINIIEPII
                     YGPN"
     gene            1005..1364
                     /locus_tag="PKZ29_01915"
     CDS             1005..1364
                     /locus_tag="PKZ29_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37658.1"
                     /translation="MVLTKPLIADTTYKLEGPGLKPDLSNPAKPLEGFLSQIIGILTI
                     VAVIYFIIQVIFAGYSFISAQGNKDKIEAARSRLTNGILGLTIIVVALGVGAFIASLL
                     GIKDPLSIQNIMPKNQP"
     gene            1366..1719
                     /locus_tag="PKZ29_01920"
     CDS             1366..1719
                     /locus_tag="PKZ29_01920"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37659.1"
                     /translation="MIKIIPPIFAQINNPVLPDSNSIVSNPSNYVNNAIQSIISIFFI
                     VAVLYFIWHFIMYAYHMIASQGNPDKWKQAQSSILYAFIGLILVFAIFAILKFVGVVF
                     GIPNLQNLQLVWPSL"
     gene            1770..2150
                     /locus_tag="PKZ29_01925"
     CDS             1770..2150
                     /locus_tag="PKZ29_01925"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37660.1"
                     /translation="MLNKLKQQIQYAAIAAPIYAQETLKIESKTQDFKPLSDLTAGGI
                     VSGAVSLVLIVVALVFFFILVWGGLKWVTSGGDEKKVGEARAQITNALIGLAIVFAAW
                     AIMKLIGTIFGIDILKGLTIPTFK"
     gene            2217..2573
                     /locus_tag="PKZ29_01930"
     CDS             2217..2573
                     /locus_tag="PKZ29_01930"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37661.1"
                     /translation="MNSIIKFFLPHPALAQTKAWSGNCVSDGVATIKGLECLFQNIIT
                     VIVSLAGFVSLAMLIVGGFKYLTSGGDPKKVAAASTTLTYAVIGIIGIIVSWLILLFI
                     KEFTGIDVTIFKIDSN"
     gene            2574..4808
                     /locus_tag="PKZ29_01935"
     CDS             2574..4808
                     /locus_tag="PKZ29_01935"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HOG37662.1"
                     /translation="MIKKIVLIISFFSLFWLTPTTVTAQSDPPSGNPPQEQSSPGFFQ
                     TIWEAITRFFNYLIFGIQYVSEDYQASTKDTRHLTNDYTRDDKDIASRAIPESVKRYR
                     KGVWFYEVLTKPHKYKNETIKSDQDICPDMKISDIAYVMYQQNQKILYQQGNPDQPLE
                     YDPNLMNQFRFEIKHPLSCYLNAYNNIQDVPQGLFMNEADAAPITSLQLNDTIRNVMP
                     RNFQGENAPPSNDNPQQAKKVAEDSDKQEQNMLLGLLSEPVHDQIACNNNADFNRENL
                     RQSFACHLTPNGWNNSKCGALGNLINTDQIQCDEEYTVKNGPGLSQRGTHGLALSGYN
                     YKEIINIYHGGGIDTERQGDDDKGPIKVVIIEDYGGDSDITCPNLVGDQNYDKVDLYQ
                     GSGGVNEDGKTPCDKYVKIPVTDPDNPSSPPYRLEGICYPVVTVPDLYTYLLGVAEVP
                     RNWHIEAQKALTLTHRHTAIQNGDKGFLWNNNKDQAFRCDQVSLNQPKIINGKEVITN
                     QGAAVELTRGEYVVDQNSRQRLHTTARSAFCGPGSYNPKFDGLKYEKISYAFDANGGA
                     INGICFEGIGYTLPSITNISTNTNSQGLISFSNNSSPGRINQVYDLAQIDGGKYLKTY
                     QGNCQLDERIFSSLNQMISAFNTDNPDNQLSFFSCYRSLNQQTDLWQENLAQNNGDQY
                     QTISQINYPGTSPHHTGRAIDFADKNGKLTSSSSAYKWLTANAHQYGFYNHQLEPEHW
                     DYNP"
BASE COUNT         1404 a         1260 c          754 g         1566 t
ORIGIN      
        1 ttactaccga cgccaaagtt ggcaccactt caatcacttc agctgtctca gttgctacca
       61 ttcctgtcgg catcggactc aaactcaaac tttttaccat cacctctgtt gatgttatca
      121 acctttccga tttccttgcc tcaaccttca ttaccaaacc taaaaaactt atgcttaata
      181 ctatatataa tagggcagtt atttttttca tattttaatt ctactttaaa accttataaa
      241 cttaaacttc acaaaagttt attaaagtgc cgtattatag cactagttta accaaatcaa
      301 cccttattgt catcaattca ttttttttct tctttagggt ataattttgc caatgacatc
      361 atctctttta gctcaagtca atatcggcaa caatcttctt gacaacagtg gcaccgccgt
      421 cggtcctaaa tataacaccg ctagccccct aatcaactct cttctcaaaa actctcttac
      481 cttagccgga attatttttc tttgtttgct tatttttggt ggcatcagca tgattatcaa
      541 cgccggttcc gctgattcta aaaaaaccgc ccaagctcaa caggccatca gtagtgctct
      601 tatcggtttt gccgtcgttt tttctgctta ttttatcatt caaattatta gtttggttac
      661 tggcgtcaaa attcttgatt ctggtttata ataacttatg ctcgcaatct ttggtagcat
      721 ctccaatcca accaattaca acagtcaagg cggttctgga ttatttcttc tcttatccaa
      781 tattttcaaa ctggccggtg ttattgccgg tcttttcttt gtggcccaat taattatagc
      841 tggttacggc tatctttcca gtagtggtga tcccaaaaaa actgaagccg cccggaataa
      901 aatcacccaa agtcttatcg gtctaattat tgttgccgga gctttcattc ttaccagtgt
      961 cgttggctca attcttaaca ttaacattat tgaacccatc atttatggtc ctaactaaac
     1021 ccttgattgc tgatactact tacaaacttg aaggtcctgg tctcaaacca gatttaagta
     1081 atcctgccaa acctcttgaa ggttttctca gtcaaatcat tggaattctg actatcgttg
     1141 ctgttattta cttcattatt caggttattt ttgctggtta ttcatttatt tccgcccaag
     1201 gaaacaaaga taaaattgaa gccgcccgta gtcgtcttac caacggtatt cttggcctga
     1261 ccattatcgt tgtcgctctt ggtgttggcg ctttcattgc cagtcttcta ggtatcaaag
     1321 atccgcttag tatccaaaat attatgccta aaaatcaacc ttaatatgat taaaattatc
     1381 ccacctattt ttgctcaaat taataatcct gttctccccg actctaacag cattgtttct
     1441 aatccctcta attatgtcaa taatgctatt caaagcatta tttctatttt tttcatcgtc
     1501 gctgttctct acttcatctg gcactttatt atgtatgctt atcatatgat tgcttctcaa
     1561 ggcaatcccg acaaatggaa gcaagctcaa agttcaattc tttatgcttt tatcggtcta
     1621 attctagtct ttgccatttt cgccattctt aaatttgttg gtgttgtctt tggtattcct
     1681 aatctccaaa atcttcaatt agtttggcct tctctttaat atttttagaa aactgttatt
     1741 attaattaat taattattat aattaaaata tgctcaacaa actcaaacaa caaatccaat
     1801 acgcagctat tgctgcccct atttacgccc aagagactct taaaatagag tcaaaaactc
     1861 aagattttaa acctcttagt gaccttactg ctggcggtat tgtttctggt gctgtcagtt
     1921 tggttctaat tgtagtcgct ttagttttct tcttcatttt agtttggggc ggtcttaaat
     1981 gggttacttc tggcggtgat gaaaaaaagg tcggcgaagc ccgcgctcaa attaccaacg
     2041 ctctcattgg tttggctatc gtctttgccg cttgggccat tatgaaactt atcggtacca
     2101 tctttggtat tgacattctc aagggtctaa ctattcccac tttcaaataa aattagttat
     2161 cttttaaaac ccgcctctcc ggcgggtttt ttgttggtct attgtataat taatttatga
     2221 attcaatcat caaatttttt ttacctcacc ccgcactagc ccaaactaaa gcctggtctg
     2281 gcaattgtgt ttccgacggt gtcgccacca tcaaaggcct tgaatgtctt tttcaaaaca
     2341 tcattactgt cattgtcagc cttgccggct tcgtctcctt agccatgctc atcgtcggcg
     2401 gtttcaaata tctcacctct ggcggcgacc ccaaaaaagt tgctgccgct tccaccaccc
     2461 tcacctacgc tgtcattggt attattggca tcattgtttc ctggttaatc cttcttttca
     2521 tcaaagaatt taccggtatc gatgtcacca tattcaaaat cgactctaat taaatgatca
     2581 aaaaaattgt tctcattatc agttttttct ccttgttttg gctcaccccc acaactgtca
     2641 ctgctcaatc agatcctcca tccggcaacc ccccccaaga acaatcatct cccggatttt
     2701 ttcaaactat ctgggaagct atcaccagat tttttaacta cctcatcttt ggtattcaat
     2761 atgtttccga ggattaccaa gcttccacca aggatactcg tcatctcacc aacgactata
     2821 ctcgagacga caaagatatt gcctcccgcg ccattcctga atcagtcaaa agataccgca
     2881 aaggcgtttg gttttatgag gtactgacta aacctcacaa atacaaaaat gaaaccatca
     2941 aaagtgatca agatatctgt cccgacatga aaatctctga tattgcctat gtcatgtacc
     3001 aacaaaacca aaaaattctc tatcagcaag gtaatcccga ccaaccgctt gaatatgacc
     3061 ccaatcttat gaatcaattt cgtttcgaaa tcaaacatcc tctttcctgt tatctcaacg
     3121 cttacaacaa tattcaagac gtccctcaag gtctcttcat gaacgaagcc gatgccgccc
     3181 ctattaccag tctccaactc aatgacacta ttagaaatgt tatgcctcgc aatttccaag
     3241 gtgaaaatgc ccctcccagc aatgacaatc ctcaacaagc caaaaaagtt gccgaagata
     3301 gtgacaaaca agaacaaaat atgcttttag gtctactttc cgaaccagtt cacgaccaaa
     3361 ttgcctgtaa caacaatgct gattttaatc gtgaaaatct tcgtcaaagt ttcgcctgtc
     3421 accttactcc caatggctgg aataactcta aatgtggcgc tctaggcaat cttatcaaca
     3481 ccgaccaaat tcaatgtgat gaagaataca ctgtcaaaaa tggacctggt cttagtcaac
     3541 gtggtaccca tggtttagct ctcagcggct acaattacaa ggaaattatc aatatttatc
     3601 acggtggtgg tatcgacacc gaacgacaag gtgacgatga caaaggaccc ataaaagtcg
     3661 ttattatcga agactacggt ggtgacagcg atattacttg ccccaatctt gttggtgacc
     3721 aaaactacga taaagttgat ctttaccaag gcagtggcgg tgtcaacgaa gacggtaaaa
     3781 ccccctgtga taaatatgtc aaaatccccg tcactgaccc agacaatccc tcatccccgc
     3841 cctatcgcct cgaaggtatt tgttatcctg ttgtcactgt tccagatctc tatacctacc
     3901 ttctaggtgt tgccgaagtt ccccgcaatt ggcatatcga agctcaaaaa gctcttaccc
     3961 tcacccatcg ccataccgct atccaaaacg gagataaagg tttcctttgg aataacaaca
     4021 aagaccaagc ttttcgttgt gatcaagtct ctctcaacca acctaaaatt atcaatggca
     4081 aagaggttat taccaatcaa ggcgccgccg tcgaacttac tcgtggtgaa tatgtcgtcg
     4141 accaaaacag ccgccaacgt ctccacacta ccgctcgcag tgctttttgc ggtcctggtt
     4201 cttacaatcc taaatttgat ggccttaaat acgaaaaaat ttcctacgcc ttcgacgcta
     4261 atggaggggc catcaatggt atttgcttcg aaggtatcgg ttataccctt cccagtatta
     4321 ccaatatttc taccaatacc aattctcaag gtttaatttc attctccaat aactcttcac
     4381 ctggtcgaat taatcaagtt tacgatctcg cccaaattga cggcgggaaa tatctcaaaa
     4441 cctaccaggg taattgtcaa ctcgacgaac gcatcttttc cagccttaac caaatgattt
     4501 ccgccttcaa taccgacaat cccgacaacc aactctcctt ttttagttgc taccgaagtc
     4561 taaaccaaca aaccgacctt tggcaagaaa acctcgccca aaacaacggc gaccaatatc
     4621 aaactatcag tcaaatcaat tatcctggca cttcgcccca ccacaccggc cgcgccatcg
     4681 attttgccga caaaaacggc aaactcactt ccagttcctc cgcttacaaa tggctcactg
     4741 ccaatgctca ccagtatggt ttttacaatc atcaacttga acccgaacac tgggattaca
     4801 atccttaaaa tatgaaaact cttgctaaaa ataaatatct cattatttta actgttattg
     4861 tcattgctct taccatcatc aaactctcct cttctcattc ttccaccact tcacctgaca
     4921 attttgacac cacccaaccc ggtcaattag ctcctcaaca aaccaaccaa aatcccattt
     4981 ctca
//
LOCUS       DAOSHV010000038         4905 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_15363_length_4905_cov_6.389278, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000038 DAOSHV010000000
VERSION     DAOSHV010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 4905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4905
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15363_length_4905_cov_6.389278"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            84..1373
                     /locus_tag="PKZ29_01940"
     CDS             84..1373
                     /locus_tag="PKZ29_01940"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOG37663.1"
                     /translation="MQRLRDAVYNILRWSEKYVKTDMIYLTKGGFWLLFSYSIQAITG
                     LILSVSFANLLAKESYGFYQFVISMASILAVFSLTGIGTAIIRGIAQGDEGCLRPSVK
                     VSLKWTLGILLTAAATSLYYWTKGNYQLSIAFAVVAIAQPLITTFNLYKTYLQGKKLF
                     KSYSVIQTAQRLLPFTLLLIAILCTRNPLTLVIVYFISHAISAVLIYLYVVIKFKLPK
                     QSEVKDLVKYGKHLSLMESMAELATAADKVLIWFFLGAAPTAAYALAQLPIIHLQAMF
                     GFIRELAFPKIAQKSLPGLAKALPEKIRTYWIVALAVVVLYIISAPTIFHIFLPKYPE
                     AVIYTQVLSLVILVVPRSLVTHTFAAHQRTKELYLVNFSTPIIRLSLLAIGLPMFGIW
                     GVIGATLISEWCAALLQWKLFSNIINKISWPKQTKIK"
     gene            1346..2284
                     /locus_tag="PKZ29_01945"
     CDS             1346..2284
                     /locus_tag="PKZ29_01945"
                     /inference="COORDINATES: protein motif:HMM:TIGR01444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HOG37664.1"
                     /translation="MAKANKDKIEQKLESRDFYKKLKLAQNFEKYSGGGLKRKLQRIL
                     YASKIYLPYLLVVKAGIGKKGTSKTKLFWGRKIEIPLTDFDSLALRTYGFAGGTEAEL
                     KLTKFFIKNLKQEDVFYDIGANYGFFTYLAEELCQEVHSFEPIPSLSEIIKNNSRNNT
                     RVNNLAISNQKGVTKLFMSESTGLSTINESATKIHTYSYDQAKEIEVKTDSLDAYCEK
                     FNKPTILKIDVEGAEGEVVDGATFFLQNHAPVIAMEIWGEGNGGDISKVAVARLRKLG
                     FESYQIEPTGNIQKVEGDITRLIPAMGVDNFIFKKN"
     gene            complement(2281..3063)
                     /locus_tag="PKZ29_01950"
     CDS             complement(2281..3063)
                     /locus_tag="PKZ29_01950"
                     /inference="COORDINATES: protein motif:HMM:NF021601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2334 domain-containing protein"
                     /protein_id="HOG37665.1"
                     /translation="MRFIIRDDDLNYFSKPSDIERWYSDIFSKNIPVGFAAIPFVRKD
                     SDVYVGAGSSGQEYPISGNLELVNYVKQNKNIEILQHGCFHENTNGVFEYAKSGGLFE
                     PTQKGKAELEKAFGSSVGIFVPPHDWISAHGILAIEDSQMDIIRGRGAGLRNFIWRKE
                     YLKNFFKMLFFRFPNYISVKTRVYPWVLDFGRHREMCSYRLEDPDIFAGLDYVNKRDG
                     VFVVVCHLHYFDEKKKNRLNDLIAKARAFGAQFSLPGEIFKT"
     gene            complement(3099..3866)
                     /locus_tag="PKZ29_01955"
     CDS             complement(3099..3866)
                     /locus_tag="PKZ29_01955"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOG37666.1"
                     /translation="MEKEELINLGLESRGGYHEKMSLKYNHSNKFDYIIASSDFVKKS
                     YIEAGYPADKIYLANFDIDFSRFDVKEPHDKTKIFKVLYMAYTTPLKGLHYLLEAWEE
                     LRLPNVELTLVGGFDSLPNKLRERYEKIIARNKNIVVVGHTPNPEEYYKDASLFVFPS
                     LTESYGRVTLEAMACGVPIITTENAQGIVEDGKTGFVVPIRDSRMIKEKIEYLYHNPE
                     IRKKMGVEARVAVENKKPFGKSVYDICQEIIGKHQTN"
     gene            complement(4268..>4905)
                     /locus_tag="PKZ29_01960"
     CDS             complement(4268..>4905)
                     /locus_tag="PKZ29_01960"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HOG37667.1"
                     /translation="KCKDYTMVAADRSYALFEAVKYVVGAAVPGDFVECGVWRGGQSM
                     LVAHTLIKLNQFDRTLWLFDTFAGMSRPTEQDVYLVTGETAESLWEADKKDDHNNWCY
                     ASLEEVQKNVSTTNYPKDKINFVKGKVEDTIPKIMPEKISILRLDTDFYESTYHELTH
                     LFPRLEKGGVLIIDDYGSWKGSKEAVDQYFKENGIQMLLFRVGNGRFGIKV"
BASE COUNT         1451 a         1083 c          883 g         1488 t
ORIGIN      
        1 ttcaaattca tatgattcag actataccac atttttttag caaaatctag aaaaatgcct
       61 tgatttgttg taagataagg ctcatgcaaa gattacgaga tgctgtttat aatattctcc
      121 gttggagcga aaaatacgtc aagaccgaca tgatttatct aaccaagggc gggttctggt
      181 tattatttag ttattcaatc caggcaatta ccggactaat tttatccgtt tctttcgcca
      241 acctgctggc aaaagaaagt tacggatttt atcagtttgt aatttcaatg gcctctattt
      301 tggctgtctt ttcattaacc ggcatcggaa cagcaattat aagagggata gcccagggcg
      361 acgaaggctg tttacggcca agcgtcaaag tttctcttaa gtggacgctc ggcatattac
      421 ttaccgcagc ggcaacttcg ctttattact ggacgaaagg aaactaccaa ttgtcgatag
      481 cttttgctgt tgtggctata gcccaaccac ttatcacgac atttaattta tataaaacct
      541 acttacaggg aaagaaactt tttaagagct attccgtaat tcaaaccgcg caaagactcc
      601 tgcctttcac tctgctactc atcgcaatcc tttgcactcg caacccgctc accttggtta
      661 ttgtctactt tatttctcat gcaatctcgg cagtcctcat ttatctatat gtcgtgataa
      721 agtttaagtt gccaaaacag tcagaagtca aagacctcgt taaatatggc aaacacctca
      781 gtctaatgga atcaatggcc gaattggcca cggccgccga caaggtttta atttggtttt
      841 ttctgggggc cgcgccgaca gctgcctatg ccttagccca attgccaata attcatctcc
      901 aggccatgtt tggttttatc cgcgaattgg ccttccccaa aattgcccaa aaaagtttgc
      961 ccgggctcgc caaagccctt ccggaaaaaa taaggaccta ctggatagtc gccctggccg
     1021 tggtcgtttt gtatataatt tctgctccaa ctattttcca tatcttcttg cccaaatatc
     1081 cggaagctgt gatttatacg caagttttgt cattagtaat tcttgttgtt ccaaggtcat
     1141 tggtcaccca cacttttgcc gcccaccaaa gaactaagga gctctacctt gttaattttt
     1201 caacgccaat tatccgtctc tccttgttgg caattggttt gccaatgttt ggcatctggg
     1261 gagtgattgg cgcaaccctt atttcagagt ggtgcgcggc cttgttacaa tggaaattgt
     1321 tttctaatat cattaataaa atatcatggc caaagcaaac aaagataaaa tagaacaaaa
     1381 gctagaaagc agagactttt ataagaaatt aaaattggct caaaattttg agaaatattc
     1441 cggcggtggc ctaaaaagaa aacttcaaag aattctttac gcctccaaaa tttatctccc
     1501 ctatttactg gttgttaagg caggaattgg caaaaaagga acttcaaaaa caaaattatt
     1561 ttggggccga aaaatagaga tacccttaac tgactttgat tctctagctc ttcgcactta
     1621 tggattcgcc ggcgggactg aagctgaact aaagttaaca aaatttttta ttaaaaatct
     1681 taagcaagag gacgtttttt atgacatcgg cgccaactac ggcttcttta cttacctggc
     1741 cgaagaacta tgtcaagagg ttcattcttt tgagccgatt ccgagcttgt cagagattat
     1801 aaaaaataac agtcggaaca acacccgggt taataattta gccatctcca atcaaaaagg
     1861 agttactaaa ctttttatgt ctgaatcaac gggtctgagc accatcaatg aatcagctac
     1921 taaaatccac acttattcat acgaccaagc caaagagatc gaggtaaaaa cagacagtct
     1981 tgacgcttac tgcgagaagt tcaataaacc gactatactc aaaattgacg tggagggggc
     2041 cgaaggtgag gtcgttgatg gtgcaacatt ttttttgcaa aatcacgcgc cggttatcgc
     2101 catggaaatc tggggcgagg gtaatggcgg cgacatttcc aaagtggccg tggccaggtt
     2161 aagaaaactt ggttttgaaa gttaccagat agaaccaact ggcaatattc agaaagttga
     2221 gggagatatc accagactga tacctgctat gggcgtggac aattttatct ttaagaaaaa
     2281 ttaggtttta aaaatctctc caggcaaact gaattgagcg ccaaatgctc gcgccttggc
     2341 gattaaatca tttaaacgat ttttcttttt ctcatcaaaa taatgaagat gacagacgac
     2401 aacaaaaaca ccatcacgtt tattaacata atctaaaccg gcaaatatat ccgggtcttc
     2461 caaccgataa ctgcacatct ctcggtgtcg cccaaaatca agcacccagg gatagactct
     2521 tgtcttgacc gaaatataat ttgggaaacg gaaaaagagc attttaaaga aattctttaa
     2581 gtattctttt cgccaaataa aatttctcaa tccggcgccc cggccacgta ttatatccat
     2641 ctgggaatct tcgatcgcca atatcccatg agcagaaatc caatcatgcg gagggacaaa
     2701 tattcccacg cttgaaccaa aagccttctc caactcggcc tttccttttt gagttggttc
     2761 aaacaggccc ccacttttgg catactcaaa tactccgttg gtattctcat ggaaacaacc
     2821 atgctgtaga atttcaatat ttttgttttg ctttacataa tttacaagct ccagatttcc
     2881 gcttattgga tattcttgtc cgcttgatcc agccccaaca taaacatcgc tatccttcct
     2941 tacaaatggt attgccgcaa agccaaccgg aatattttta gagaaaatat cagagtacca
     3001 acgttcgata tcgctaggtt tactaaaata atttaggtca tcatctctaa ttataaatct
     3061 cattgctttt ccttattcgt agcgtttaaa tattaacttc aattcgtttg gtgtttccct
     3121 ataatctctt ggcaaatatc atagaccgat tttccaaacg gtttcttgtt ctcaaccgcc
     3181 accctcgctt ccacgcccat tttttttctg atctccggat tatgataaag atattctatt
     3241 ttttccttta tcatccggga atctcttatc ggaacgacga accctgtctt cccatcctca
     3301 acaatccctt gagcgttctc ggtagtgata atcggcaccc cgcaggccat ggcctccaag
     3361 gtcacccgac cataactttc cgttagtgat ggaaatacaa aaagagaggc gtccttgtag
     3421 tactcttcgg gattaggtgt atgtcccacg actacaatat ttttgttccg ggcaattatt
     3481 ttttcatatc tttctcttaa tttatttgga agagaatcaa aacctccaac caaagtaagt
     3541 tcaacatttg gcaatctaag ttcttcccag gcttcaagca aataatgaag tccctttaaa
     3601 ggagtcgtat atgccatata aagcacttta aatatttttg ttttatcatg aggctctttt
     3661 acgtcaaatc ttgaaaaatc aatatcaaaa ttagccaagt atattttatc ggcagggtaa
     3721 ccggcctcaa tgtatgactt tttgacaaaa tccgagctgg caattatata gtcaaattta
     3781 tttgaatgat tgtatttcaa actcatcttt tcatgataac cccctctact ttcaagtcct
     3841 aggttaatta attcttcttt ttccaatctg gcatttgtgt tgggatgggc aagccgcggg
     3901 atgtctacag taaccgaccc tgcctttgag gctttttcaa gtgtccttct taaaaaatgg
     3961 cccccatgga ataaaactat ttcagagggt cttaatctta ggctggcgga ccaatcaaat
     4021 attttttcat agaaaaatct agaaattttt attttaaaat atttttcggc tattctaaat
     4081 aaataatgca acggcaaggg aatagcagaa taggtgcggg gaaaagaaaa atttcggtta
     4141 gtgtaacaaa cgacctgttc taactcaccg ccagccgaaa attccttaat aaccggcaat
     4201 atatcagggg ttgaaatact gattttctgc ccaagacttg aataggttat aacggaaatc
     4261 gccatactta aacttttata ccaaatctac cattaccgac cctaaaaagg agcatctgaa
     4321 tgccattttc tttgaaatac tggtctaccg cctcttttga acctttccac gagccgtaat
     4381 catcaataat tagtaccccg cctttttcaa gccgcgggaa aagatgtgta agttcatgat
     4441 aagtagattc ataaaaatcc gtgtccaatc gcaaaataga tattttttct ggcattattt
     4501 taggaatagt atcttcaact tttcccttaa caaaattaat tttatctttg gggtagttag
     4561 tggtactgac atttttctgc acctcttcaa gtgaagcata gcaccagttg ttgtgatcat
     4621 cttttttgtc cgcttcccat agcgattccg cggtttcccc ggtgactaaa tagacatctt
     4681 gttctgtggg tctactcata cccgcaaagg tatcaaacaa ccatagagtt cgatcaaatt
     4741 ggttcaactt aattagggta tgcgcaacca acatcgattg gcctcctcgc cagactccac
     4801 actccacaaa atcaccggga accgcagccc caacaacata tttcactgcc tcaaaaaggg
     4861 cataagacct gtctgcggca accattgtat aatctttgca ttttt
//
LOCUS       DAOSHV010000039         4753 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_16052_length_4753_cov_4.976160, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000039 DAOSHV010000000
VERSION     DAOSHV010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 4753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4753
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16052_length_4753_cov_4.976160"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2621)
                     /gene="ileS"
                     /locus_tag="PKZ29_01965"
     CDS             complement(<1..2621)
                     /gene="ileS"
                     /locus_tag="PKZ29_01965"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOG37668.1"
                     /translation="MSKFKNIPQNIKLTDLEEDTIKFWQDKKIFEKSVSSRPEDNSYS
                     FYDGPPFITGSPHHGSLLPGIAKDVIPRYWTMKGKRVRRVWGWDCHGLPAENKVEELL
                     GLKSKKDIEAIGIKKYIDACYSYVQNVSQEWPWYIDRTGRWVDFENAYKTMDLDYMES
                     VMWAFSEIYHKDLIYKSHRVSLFCPRCSTPLSNFEIAMDNSYKMVDDPAIFIKFKLKN
                     QDKFIVAWTTTPWTLPANLALAVDQNETYVEIILDNQTLIFAKARLDAVCGNNPYKLI
                     KEFPGKDLIGLEYEPLYHFYDTNQNDYHIYHGDLVTMTDGTGVVHIAPGFGEDDAVLG
                     KLFNLSVVNHVDDEGKISPTVIPFANLFVKKADPLVIDDLKSRNLLLKEEKINHSYPF
                     CYRCGTPLIYKTQNAWYLDVQKIKDQMIATNQKINWVPNHIKDGRFGQGISTAPDWCL
                     SRSRYWASPIPIWECDCGQRYVPSSIADLEKRSGQKITNLHKPDIDEVTVTCDKCHQT
                     VHRVPEVLDCWFESGSMPYAQLHYPFKNKEFFHQSFPADYIIEYIGQTRGWFYTLHVL
                     SNALFASESFKNCVVTGVVMGNDGRKMSKLLKNYPDPKTILQQYGGDAYRLYLMSSVV
                     MIGENINIDEKEFENQVKNILLPLFNSFKYFITYANLHRWSPKKDASESDHPLDRWII
                     NQVNGFTQNMAKSLEEYNLVNATRLVEPFVNDLSRWYIRQSRDRFVNGDQPALQTLWQ
                     VLVQFSLSAAPIIPFITEYFWQNLVVPIDSTKSESIHLEFYPQTKPVDPKITNDMFLV
                     QQICEQGNMIRKTNQISTRQPLSLLTVSSSQDLKLNQDLIDIIKNEINVKDINFVKSG
                     KNLTIDLDTNITPVLKA"
     gene            complement(2669..3832)
                     /gene="ftsZ"
                     /locus_tag="PKZ29_01970"
     CDS             complement(2669..3832)
                     /gene="ftsZ"
                     /locus_tag="PKZ29_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOG37669.1"
                     /translation="MALIKTLAGQFAKIKVIGIGGGGNNAINSMIAEDSIKGVDFIAI
                     NTDSQALLNSQASIKVQIGEKLTKGLGAGGNPQMGQKAAEESKERIKEMLVGTDMVFI
                     TGGMGGGTCTGAAPIIAEISKKELGILTIAVVTKPFLFEGTRRMTNAEEGISQLRNNV
                     DTLIVIPNQKVMEVVNDKATLLEAFKIADSVLNKGTKAIADLITVPGLINLDFADIKS
                     VMSNAGSALMGVGEADGEDRARKAVEAAVESPLVEATIEGARGVLINVTGGPDLTMAE
                     IEESARNITQRAAPDANIIFGATIDPELKGKIKISVIATGFDTTKANFYQSLKKPTPV
                     VESNPLPQTKSDDNQPAPIDNKDIKKFLGDKDIPAGIDIVDEFDIPAFLRKKG"
     gene            complement(3834..>4753)
                     /gene="ftsA"
                     /locus_tag="PKZ29_01975"
     CDS             complement(3834..>4753)
                     /gene="ftsA"
                     /locus_tag="PKZ29_01975"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOG37670.1"
                     /translation="RVVEAAKAVAIPNGKEIIHIIPICYTVDGQEGVIDPIGMNGVRL
                     EVESHIIIASTPSLKNLKKCLEDIGIKIDQLVYSGLATAKSTLTETEKELGVALVDIG
                     GSITTITIFNEGSPVYSSVIPVGGSNVTNDLAIGLRFSLEDAEKIKLRLNKVSESKKF
                     EDEIDLSAFGLPADDKRKISLRTAVNGIINPRLEELFHLIYDKIEESGFHQTIPAGIV
                     LTGGGAQTINIKEVCEKVIPLPLRIASPPKVGGIIDDIINPAYSSSIGLLMFINENKK
                     ITPSSVKKPKLQVGALFGKIKKLIEPLMP"
BASE COUNT         1307 a          883 c         1135 g         1428 t
ORIGIN      
        1 tcggctttta gcacgggggt gatattagtg tctaaatcta tagttaaatt tttccctgat
       61 ttgacaaaat ttatgtcttt gacattgatt tcgtttttga tgatgtcaat taaatcctga
      121 ttaagtttaa gatcttgaga agatgaaaca gtgagaagag aaagcggttg gcgggtgctg
      181 atttggttgg ttttgcggat catattcccc tgctcacaga tctgctgaac tagaaacata
      241 tcattggtaa ttttggggtc gacgggtttg gtttggggat aaaattccaa atgaatagac
      301 tccgacttgg ttgagtcgat gggaacgact aaattctgcc aaaaatattc agtaataaag
      361 ggaatgatgg gagcagcgga aagactaaat tggactaaca cctgccatag ggtttgcagg
      421 gctggttggt cgccgttgac aaatctatcg cgcgactgac ggatatacca gcgtgacagg
      481 tcgttgacaa acggttcgac aagtcgggtg gcgttgacta agttatattc ttccagcgat
      541 ttggccatat tttgggtaaa gccgttgact tgattgataa tccagcgatc aagcggatgg
      601 tcggattcag aggcgtcttt tttgggtgac caccgatgga gattggcata ggtgataaag
      661 tatttgaaag aattaaatag tggcaggaga atgtttttga cttgattttc gaactctttt
      721 tcgtcgatgt taatattttc gccaatcata accacactgc tcataagata aagacggtaa
      781 gcatcgccgc catattgctg aagaatagtt ttgggatcgg ggtagttttt gagaagttta
      841 ctcatttttc ggccgtcatt gcccataacg acaccagtaa caacacagtt cttgaaagat
      901 tcggaagcaa aaagagcatt ggacaagaca tgaagagtgt aaaaccagcc gcgggtttga
      961 ccgatgtatt caataatata atctgccggg aagctttggt ggaaaaattc tttgtttttg
     1021 aaaggataat gaagctgggc ataaggcatc gaaccggatt cgaaccaaca gtctaaaacc
     1081 tcgggaacac ggtggacggt ttgatgacat ttgtcgcaag taacagtaac ttcgtcgata
     1141 tcgggtttgt gaaggttggt gattttttgt ccggatcttt tttcgaggtc ggcaatactg
     1201 gaggggacat atctctgacc acaatcacac tcccagatag gaataggaga cgcccaatac
     1261 cgggatcgag acaaacacca atcgggggcg gtagaaatcc cctgcccaaa acgaccatct
     1321 ttgatatgat ttggaaccca attaattttt tgattggtag ctatcatttg gtctttgatt
     1381 ttttgaacat caagatacca ggcgttttgg gttttataga tcaaaggagt gccgcagcga
     1441 tagcagaaag gatagctgtg attaattttt tcttctttta gtaataaatt tcgggatttg
     1501 aggtcatcaa tgaccaaagg atcggctttt ttgacaaaca gattggcaaa aggaatgacg
     1561 gtggggctga ttttgccttc atcatcaaca tgattaacaa cagagagatt aaaaagtttg
     1621 cccagaacgg catcatcttc accaaatccg ggggcaatat gaacgacacc ggtgccgtcg
     1681 gtcatggtga ctaaatcgcc gtgataaata tgatagtcat tttggttagt gtcataaaaa
     1741 tgatacaaag gttcgtattc taaaccgatg agatccttgc caggaaattc ttttatgagt
     1801 ttatagggat tattgccgca gacagcatca agtctggctt tggcaaaaat aagggtttgg
     1861 ttgtctagaa taatttcgac ataggtttcg ttttggtcaa cagctagggc taagttggct
     1921 ggcagcgtcc aaggggtagt agtccaggca acaataaatt tatcctgatt ttttagctta
     1981 aatttgataa aaattgccgg atcatccacc attttgtagg aattgtccat agcaatttcg
     2041 aaattagaca aaggagtaga gcatcggggg cagaaaagag aaacgcggtg acttttgtaa
     2101 atcagatcct tgtgatatat ctccgaaaag gcccacataa cactttccat gtaatctaaa
     2161 tccatggttt tgtaggcgtt ttcaaaatca acccatctgc cggttcgatc aatataccaa
     2221 ggccattcct gggagacgtt ttggacatag gaataacagg catcaatata ttttttgata
     2281 ccaatggctt cgatatcttt tttgcttttt agtccgagaa gttcctcaac cttgttttca
     2341 gcaggaagac cgtgacaatc ccagccccaa acgcgtcgga cacgtttgcc tttcatggtc
     2401 caataccgag gaataacgtc tttggcaatt ccgggcaaga gactaccgtg gtgaggagag
     2461 ccggtaataa aaggaggtcc gtcatagaaa ctatagctat tatcttcggg tcgagatgaa
     2521 actgatttct caaaaatttt tttgtcttgc cagaatttta tggtgtcttc ttcaagatcg
     2581 gtaagtttta tgttttgggg aatatttttg aatttagaca tactctattt aagagatgat
     2641 tatataacaa aattttgttt gagtaaattt agcctttttt tctgagaaag gcaggaatat
     2701 caaattcgtc gacaatatca attccggcag gaatatcttt atcacccaaa aattttttta
     2761 tatctttgtt gtcgattggg gctggctgat tgtcgtcgga tttggtttgt ggtaagggat
     2821 tagattcaac caccggggtt ggttttttga gagattgata aaaattggct ttggtggtgt
     2881 caaatccggt ggcgatgaca gaaattttta ttttgccttt gagttcgggg tcgatggtgg
     2941 cgccaaaaat aatattggcg tcaggagccg ctctttgggt gatgtttcgg gctgactctt
     3001 caatttcggc catggtcaaa tcgggtccgc cggtaacatt gattaggact cccctagccc
     3061 cttcgatagt ggcttcaacc aagggcgatt cgacggcggc ttcaactgct tttctggctc
     3121 tgtcttcgcc gtcagcctcc cctactccca taagagcaga gccggcgttg ctcataaccg
     3181 atttgatatc ggcaaaatca aggttgatga gtccgggaac ggtaattaag tcggcaatag
     3241 ctttggtgcc tttattgagg acagaatcgg caattttgaa agcttcaagg agagtggctt
     3301 tgtcgttgac aacttccata actttttggt taggaatgac gatgagagtg tcgacgttgt
     3361 ttcggagttg ggaaatacct tcttcagcgt tggtcatacg gcgagtgcct tcaaacaaaa
     3421 atggtttggt gacaacggca atagtaagga tacccaattc ttttttggaa atttcagcaa
     3481 taatcggagc tgcaccggtg caggtgccgc cacccatacc gccggtgata aaaaccatat
     3541 cggtgccgac tagcatttct ttgattcttt ctttggattc ttcggcagct ttttgtccca
     3601 tttgcggatt gccgccggca cccaaaccct tggtcagttt ttctccaatt tgaaccttga
     3661 tagaggcttg agagttgaga agggcttgag aatcggtgtt gatagcaata aaatcgacgc
     3721 ctttgatact gtcttcggcg atcatagaat tgatagcatt gttgccaccg ccaccaatac
     3781 caatgacttt aattttggcg aattgtccgg caagggtttt tatcagagcc atattaaggc
     3841 attaagggtt caattaattt tttaattttt ccaaacaggg ctccgacctg aagttttggt
     3901 ttcttgaccg aagaaggagt aatttttttg ttttcgttga taaacataag tagaccgata
     3961 gaactgctgt aagctggatt gatgatgtca tcaataatac caccgacttt tggaggactg
     4021 gcaatccgca acggtaaggg aataactttt tcgcaaactt ctttgatatt gatagtttga
     4081 gctccgcctc cggtaagaac aatgccggca ggaatggttt gatgaaaacc actttcttct
     4141 attttgtcgt agattaggtg aaataattct tccagtctag gattgataat gccgttgacg
     4201 gcagtgcgga gcgatatttt tcttttgtca tcggcgggaa ggccaaaagc ggaaagatca
     4261 atttcatctt caaatttttt ggattcactg actttgttaa gccggagttt gattttttca
     4321 gcatcttcaa gagaaaatct aagaccgatg gccaagtcat tggtgacatt gctgccgcct
     4381 acgggaataa cagaggaata aacgggagaa ccttcattaa agatagtaat ggtagtaata
     4441 ctgccgccga tatcaaccag agcgacccca agttcctttt cggtttcggt aagagttgat
     4501 ttggcggtag ccagaccgga atagaccagt tggtcaattt tgataccaat atcttcaagg
     4561 cattttttga gatttttgag tgaaggggtg gaggcaataa taatatgaga ctcgacttcg
     4621 agacgaactc cgttcatacc gataggatca atcaccccct cctgaccgtc aacggtataa
     4681 caaataggaa taatgtggat aatttctttg ccgttgggaa tggccaccgc tttggcggct
     4741 tcgacaacac ggt
//
LOCUS       DAOSHV010000040         4643 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_16552_length_4643_cov_7.653226, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000040 DAOSHV010000000
VERSION     DAOSHV010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 4643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4643
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16552_length_4643_cov_7.653226"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2111)
                     /locus_tag="PKZ29_01980"
     rRNA            complement(<1..2111)
                     /locus_tag="PKZ29_01980"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            complement(2345..2418)
                     /locus_tag="PKZ29_01985"
     tRNA            complement(2345..2418)
                     /locus_tag="PKZ29_01985"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2382..2384),aa:Ala,seq:tgc)
     gene            2659..3303
                     /locus_tag="PKZ29_01990"
     CDS             2659..3303
                     /locus_tag="PKZ29_01990"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HOG37671.1"
                     /translation="MTQILQSILDMVKTHGLLAMFIGGVVEQIIIPIPSPIITMSGGA
                     FLIQHGLPFFQTIWEIITQVSLPYSFGAVIGTSFVYLIAYYGGRPLINKFGKYIGISW
                     NLIEKIKADFQKTIKDELFIFISVCIPVVPVSLISAFCGGFRIKPQKFYPMMFLALIV
                     RSIILGYIGFSMGEAFLGLAHGLDKIESFLTIVGAFLILGFLFLKREKWLKKNS"
     gene            complement(3300..4400)
                     /locus_tag="PKZ29_01995"
     CDS             complement(3300..4400)
                     /locus_tag="PKZ29_01995"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HOG37672.1"
                     /translation="MKKLGRILFFLVLVGLAVGMWLTKEGEFLNPLSLNRQGKDKKKE
                     LVTFGFLPTWMVGKTRVYGREVSHLIFLGVETEETGKLIWDFQSNKINNESYLRQKEE
                     TKNQGGKNILGIKLFKDDKLTKLLSSENYRKNLVEEVGRVVEEEEFDGVNVDFEYQGN
                     PTKILEKNFLTFLEELRQAEVGEISVDVFANTVIKGSATEIEELVSKVDYLVVMAYDF
                     HRPGVDYAGSVAPLGSKVGERNITEVVERYLNLQLNKDKLVLAYPLYGYEWKTYTDEF
                     ESQIKRGWYQMASWNRVKNLIKEKKIEVNWDELSMTPWLSFEEDGETRQIYFENNQSL
                     RAKIDLVRQNKLAGYGFWALGYEGEDRSIWGY"
     gene            complement(4401..4535)
                     /locus_tag="PKZ29_02000"
     CDS             complement(4401..4535)
                     /locus_tag="PKZ29_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37673.1"
                     /translation="MERKRRRLTGVVGILAAIGAGVIVVFGNLGNLLRTIKFAARNKS
                     "
     gene            complement(4567..4643)
                     /locus_tag="PKZ29_02005"
     tRNA            complement(4567..4643)
                     /locus_tag="PKZ29_02005"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4607..4609),aa:Ile,seq:gat)
BASE COUNT         1184 a         1240 c          691 g         1528 t
ORIGIN      
        1 ttaaccttct agcaccggtc aggtttcggc ccatatacat ccccttacag gttcgcatgg
       61 acctatgttt ttggtaaaca gtcgccgtgt attcttttgt tgtaacccgg gtgttatccc
      121 gggcagggca tctcgcaaag cttacgccca gttaatttgc tgatttcctt aaccatcttt
      181 cttccaatcg tcttagtctt ctcgacttat ccaccagtgt cggttttagt acggatcacc
      241 aatattctat ttgcgatact tttctcgtgt acatggattt aatcttgtct cccgataact
      301 caggattcta ttcgtgcttc accttaaaac gaaaaggcgg attttccaac cccttctagc
      361 tagacactta aaagcccaat cacttagact caaaaccttc catacaccgt cataccccaa
      421 attaaaacga atattagtaa ggaccggaat attaaccggt agtccatcca ctacgccccc
      481 attaatcagg gcctcgtgtt aggatcgcct aaccctcagt cgaacaacgt agctgaggaa
      541 accttggata tttcgacgtc ccgggttctc accgggatta cgttactcat gcctgcattc
      601 tcacttctga caactccagc ttagcttacg ccttaacctt cgccgtcacc agaacgctct
      661 cctaccattc agtgctccgc acttcaagtt caaaagttta tcaagttata aagtccataa
      721 agtatttttg atatttactt tacaaacttt aaaaactttt aactttataa actcaaggtg
      781 cgaagcacca aatctccatc ttcggcacta tgttttagcc tcgttacatt tttggtgcaa
      841 tctttcaact ccgataaatc ggagtcgaac agtgagctat tacgctttct ttaaaggatg
      901 gctgcttctg agccaacctc ctgtctgtct tagaaaaatc actccctttc ccacttaaca
      961 tagatttaga gaccttagat gagagtctgg ggtcttcccc ttttgccgat ccagcttagc
     1021 ccggaccgac tgactgccaa gttaaatttg cggtattcag agtttggtta aatgccgtgg
     1081 attgctccac aaacattcat ccagtagctt tacccccgca tctcaacact tgacgctata
     1141 cctatatata tttcggagag aaccagctat ctccagtttc gattggcata ttacccctaa
     1201 cctcaagtca tccgacattt ttgcagcaat gaccggttcg ggcctcctcc tggctttcgc
     1261 caaaattcgc cctgctcaag gttagctcaa ctggtttcgg gttatatcca gccatctaat
     1321 cgcgctcttc gcactcgctt tcgctacgcc tccactccgt tacggagctt aaacttgatg
     1381 actagaaaca ctcgcaggct cattcttcaa taggcacgac attactcgcg ctccgcgctc
     1441 gaagttcaaa agtttatcaa gttataaagt ccataaagta tttttgatat ttactttaca
     1501 aactttacaa acttttaact ttataaactg cgggtgcgaa gcaccagctc tgtctgtttg
     1561 ttagcaaatg gtttcagatc tatttcattc ccttacttag ggtgtttttc acctttccct
     1621 cacggtacta gttcactatc ggtccgctaa agtatttagc cttagagatt ggtctcccta
     1681 gattcccaca atatttcgcg tgtcttgtgg tacttaggta tctaaccaaa cagtacttaa
     1741 acttaaaaat acaggacttt caccttcttt ggtcactcat tccagagtgt ttttctaatt
     1801 tttcctgttc ttatgtcaga ccctgcaacc ccccgataaa tcggggttta ggctcttccg
     1861 gtttcgctca ccgctactaa cggaatctct tacgatttct tttcttccag gtacttagat
     1921 gtttcagttc cctgacttac cttccacgac ttgcgtcggg atcagtccga tttctcggac
     1981 caaggtttcc ctattcggac atcgttggat caaagtctgt tgacgactcc ccaacacttt
     2041 tcgcagtctt ccgcgtcctt cttcggcttt agcaaccaag gcatccgcca tttgcttcat
     2101 taaaatttta ctcaatattt cggcaactca atttagctgc caagaataaa acgaaattga
     2161 aaataaaatt acttcgattc aataaattgt taatgttctc agttcagttt aagaagataa
     2221 aaaattatct tcttattcct aaactttata aacttgaatt tttaagttta taaacttaga
     2281 gtgtggacct ggtcccgcaa cggcgtgacg accccttcat tgctgaagtg gacaaaacgc
     2341 ccggtggacc tggggagatt cgaactcccg accccttcat tgcaaatgaa gtgctctaac
     2401 caactgagcc acaggcccag aaaattaaat gtgctcttaa ataaccataa aaaaacctgc
     2461 cttttcggca ggctggccga aggttcaatg ccctcggcta attcaacttt tcttacccta
     2521 ccctcgttcg gcaaccctcc cttggttttg tttgaatatg ttgaatttag tccttagcat
     2581 tatttaagtg ggtttattct acctaaatct tccctcaacg tcaacaactt gttttacata
     2641 tagggtatac tactttttat gactcaaata cttcaatcca ttcttgatat ggttaaaact
     2701 catggtcttt tggccatgtt catcggcgga gtggttgaac aaattattat tcccattcct
     2761 tcacctatta ttaccatgtc cggcggcgcc tttcttatcc agcacggtct gccctttttt
     2821 cagaccattt gggaaattat tacccaagtc tccctcccat actcctttgg cgctgtcatt
     2881 ggcaccagct ttgtttatct tattgcctac tatggcggtc gtccgcttat aaacaagttt
     2941 ggtaaatata tcggcatcag ctggaacctg attgaaaaaa tcaaagccga cttccaaaaa
     3001 actatcaaag acgaactctt tatctttatt tccgtttgta tccccgtcgt tcccgtctct
     3061 cttatctctg ctttttgcgg cggtttccgc atcaagcccc aaaaattcta tcctatgatg
     3121 tttttggctc tcatcgtccg ctctatcatt cttggttata ttggttttag tatgggtgaa
     3181 gcctttctcg gtttagctca cggcttggac aaaattgaaa gttttctcac tattgtcggc
     3241 gcctttctta ttttgggatt tctctttctc aaacgggaaa aatggctcaa aaaaaacagc
     3301 taataacccc aaatacttct gtcttcccct tcatatccca acgcccaaaa accataaccg
     3361 gctaatttat tttgccttac caagtcaatt ttggctctca aactctgatt attctcaaaa
     3421 tatatttgtc ttgtttcccc atcctcttca aaagacagcc agggtgtcat tgacagctca
     3481 tcccaattaa cctctatttt tttctcctta atcaaatttt tcacccgatt ccaactcgcc
     3541 atctgatacc atcctctttt tatctgtgat tcaaactcat ccgtataagt tttccattca
     3601 tagccatata acggatatgc caacaccaat ttatctttgt tcagttgcaa gttcaaatat
     3661 ctctctacta cttcggtaat atttctttcc cccactttcg accccaacgg cgctaccgat
     3721 ccggcataat ccactcccgg ccggtgaaaa tcataagcca ttactaccaa ataatctacc
     3781 ttacttacca actcttctat ttctgtcgcc gatcctttaa ttaccgtgtt agcaaacaca
     3841 tccacactta tttctcccac ctccgcctgc ctcaattcct ccaaaaatgt caaaaaattt
     3901 ttttccaaaa tttttgtcgg atttccttga tactcaaaat caacattaac cccgtcaaat
     3961 tcttcttcct caaccacccg ccctacttct tctactaaat tttttctgta attttcactg
     4021 ctcaatagtt ttgtcagttt atcgtcttta aataacttaa ttcccaaaat atttttaccc
     4081 ccctgatttt ttgtttcctc tttttgcctc aaataagact cgttattaat tttattcgac
     4141 tgaaaatccc aaattaattt acctgtttcc tctgtttcca cccccaaaaa tattaaatga
     4201 cttacttccc tgccataaac tctcgtcttc cccaccatcc aggttggcaa aaatccaaat
     4261 gttaccaact ctttcttttt atcctttcct tgccggttca aagataatgg gttcaaaaat
     4321 tcaccctctt ttgtcaacca cattcccacc gccaatccga ccagcaccaa aaaaaataat
     4381 atccttccta attttttcat ttatgattta tttctggccg caaacttaat tgtcctcaat
     4441 aaattaccca agtttccaaa aaccactatc actcccgccc ctatcgctgc caaaatccca
     4501 accactccag ttaatcttct cctttttctt tccataaatt attctatcat tcttaccccg
     4561 ttactctggt gggtgatgga gaactcgaat ctccgacccc cgtcttatca ggacggtgct
     4621 ctaaccaact gagctaatca ccc
//
LOCUS       DAOSHV010000041         4515 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_17270_length_4515_cov_5.150000, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000041 DAOSHV010000000
VERSION     DAOSHV010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 4515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4515
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17270_length_4515_cov_5.150000"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..671
                     /locus_tag="PKZ29_02010"
     CDS             <1..671
                     /locus_tag="PKZ29_02010"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOG37674.1"
                     /translation="KPMMKTMVFDIETAGKKFDDLDEVEQEYLLNNLEKNEEDKEVAK
                     TKTGLYSIFGQVCAIGAFNPNSDKGMVLAISNKELKPEKENFVYKTFETEKELLVNFW
                     QIATDYEVFVTYNGDNFDFPYLIIRSGINRVKVPFEVNRWKSDRFIDLQNKIRQNHGF
                     KLEMLCKAFGIENPKEKGVHGDDVGRLFEAKEFQKIADYVARDAAATSQLYLIWKEFM
                     SGEI"
     gene            685..840
                     /locus_tag="PKZ29_02015"
     CDS             685..840
                     /locus_tag="PKZ29_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37675.1"
                     /translation="MTKINKKDEYSLELEIVTKTEYDRPKKVDRMVQRNLKKVIMNRL
                     DGKMGEC"
     gene            complement(837..1565)
                     /locus_tag="PKZ29_02020"
     CDS             complement(837..1565)
                     /locus_tag="PKZ29_02020"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOG37676.1"
                     /translation="MTFIQSLILGLTQAVSEFLPISSDGHLNLVQHFFRLTPSLALDV
                     FLHAATFLSVLFYFRHQTKYFFDHLKYIIVGSIPAAITGLFFKDQLESLFANPKLLPY
                     FFLFTGLMVFSTKFIKPDNKKITYPMAFIIGIFQALAILPGVSRSGGTIFSALLLGLS
                     PAAAFNFSFGLFIPVSIGAILFSLKDIFSANLITPINLLIFVITGLVGIFVLSCLQKL
                     VIKNKFWIFGLYVIILSVCLFFFL"
     gene            complement(1595..2005)
                     /gene="rpsI"
                     /locus_tag="PKZ29_02025"
     CDS             complement(1595..2005)
                     /gene="rpsI"
                     /locus_tag="PKZ29_02025"
                     /inference="COORDINATES: protein motif:HMM:NF001099.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOG37677.1"
                     /translation="MAKKTSKTNYTRSIGRRKSAVATVKIFSGKGDCLVNGIQLSKYF
                     PTKTEKIIYDKPFEITKTAGKYHFSATIIGGGKTGQVQALSLAIARSLIKIDESFKPV
                     LRAGGLLTVDSRVKERRMVGTGGKARRQKQSPKR"
     gene            complement(2007..2423)
                     /gene="rplM"
                     /locus_tag="PKZ29_02030"
     CDS             complement(2007..2423)
                     /gene="rplM"
                     /locus_tag="PKZ29_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOG37678.1"
                     /translation="MKTSVLKGNQVNRNWHLIDLSNQTLGRICTQIASILMGKDKTTF
                     SYHRDDGDYVVAINAADIRVTGSKLKDKIYRRHTHWPGGLKELTLDEMLKKDPRLVIK
                     SGVYGMLPKNKLRDRRMTRLKIFTDSNHPYADKLTK"
     gene            complement(2420..2896)
                     /locus_tag="PKZ29_02035"
     CDS             complement(2420..2896)
                     /locus_tag="PKZ29_02035"
                     /inference="COORDINATES: protein motif:HMM:NF013369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L17 family ribosomal protein"
                     /protein_id="HOG37679.1"
                     /translation="MKHRNFGKKLGRNHHERQALFRSLTRSMFIHGSIKTTEAKAKSI
                     IPTIESLSNTIITKPELIAKRELFRHLQDRNWVNNVCQTFKQVFDGQTKNFTQISRVS
                     RRYGDDALIVKLSFVKPVKFAVKPEKEVVNKDKKDTKKPAVTKAKPSKKVKQETKS"
     gene            complement(2915..3832)
                     /locus_tag="PKZ29_02040"
     CDS             complement(2915..3832)
                     /locus_tag="PKZ29_02040"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF003519.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOG37680.1"
                     /translation="MIETNKFNIKTIKSTEKYGKFELSPLVGGFGHTLGNSLRRVLLI
                     TIPGAAITRIRVDGATHLFTTLPGVKEDLVEISLNLKKIKFNYAKEEPIVLKLDKKGP
                     GVVTAGDITDNDLCRVANPDQVIATLSDAKSSLKIEMTVERGVGYTMAKEQKTDIIGE
                     IILDAAFTPILKSNYTVEPTRLGKKSNYDKLTMEIWTDGSVDALYALKMAAKDLINAF
                     GQIADPKDFEEEIITINTSLSPQGSDISVEEIELPLRVTNALKKAGYSTIDTLVKSGR
                     SEVSKAKNVGEKSLKIIDSWLKERGFTWK"
     gene            complement(3846..4475)
                     /gene="rpsD"
                     /locus_tag="PKZ29_02045"
     CDS             complement(3846..4475)
                     /gene="rpsD"
                     /locus_tag="PKZ29_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016321018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOG37681.1"
                     /translation="MSRITLDAKCRLCRAAGDKLYLKGARCFSAKCPIEKRGAVKPGM
                     HGAKSSSKPTDYGIQLKAKQKAKRIYGVQEVQFKNYYLKAKKLRGLVGDNLLNLLENR
                     LDNVLYLSGLSLSRSSAKQIVGHGHALVNNQIMNIPSYQVKVGDIISLDKKVIDKIGT
                     LPADESDFKTKSWLDLNKKDFTVKVISEPLKEEIGNDIDINLIIEYYSR"
BASE COUNT         1419 a          755 c         1030 g         1311 t
ORIGIN      
        1 ataaaccaat gatgaaaacg atggtatttg atattgaaac ggcgggaaaa aaattcgatg
       61 atttggatga agtggagcag gaatatttat taaataacct ggaaaaaaat gaggaggata
      121 aggaggtggc aaaaaccaag actggattgt attcaatttt tggtcaggtt tgcgccattg
      181 gggcatttaa tcctaactcg gacaagggaa tggttttggc cataagcaat aaagaattaa
      241 aaccggaaaa ggaaaatttt gtttataaaa cttttgaaac cgaaaaagag ctgttggtta
      301 atttttggca aattgcgacg gattatgaag tatttgtgac ttataacggc gataattttg
      361 attttcctta cctaattatc cgatcgggaa taaaccgggt gaaagttccg tttgaagtaa
      421 acaggtggaa atcagaccgg tttattgatc tacaaaataa aatcagacaa aatcacgggt
      481 ttaaattaga aatgttgtgc aaggcttttg gaattgaaaa tccaaaagaa aaaggggttc
      541 atggcgatga tgtcggcaga ttgtttgaag caaaagagtt tcaaaaaatt gccgattatg
      601 tggcgcggga tgcagcagca accagtcaat tatatctgat ttggaaagaa tttatgagtg
      661 gagaaatata aattaaaaaa ctaaatgaca aaaataaaca aaaaagatga atatagtttg
      721 gaactagaaa ttgtgaccaa gactgaatac gaccggccga aaaaagttga ccgaatggtg
      781 caaagaaacc taaaaaaggt gattatgaac aggctggatg ggaaaatggg tgaatgttag
      841 agaaagaaaa aaaggcagac agacaagata atgacgtaaa gtccaaaaat ccaaaattta
      901 tttttgatga ctaatttttg gagacaggag agaacaaaaa tccccaccaa accggtgatg
      961 acaaaaatca aaagattaat tggggtaatt aaattggcag aaaaaatatc tttgaggcta
     1021 aacagaatgg cgccaataga aaccggaata aataaaccaa aagaaaaatt aaaggcggcg
     1081 gcaggagaca gtcctaaaag aagggcagaa aaaatggtgc cgccggaccg agaaactccg
     1141 ggaagaatgg caagagcttg aaatatgcca ataataaagg ccatggggta ggtaattttt
     1201 ttgttgtcgg gcttgataaa tttggtagaa aaaaccatca gcccggtaaa aagaaaaaag
     1261 taaggaagta gtttggggtt ggcaaataaa ctttctaact gatctttgaa aaacagacca
     1321 gtgatagcgg caggaataga accaacaata atatatttga gatgatcaaa aaaatattta
     1381 gtttgatgac ggaaataaaa taaaactgat aaaaaagtgg cagcatgaag aaaaacatca
     1441 agggctaaag aaggagtcag gcgaaaaaaa tgctggacta gattaagatg accgtcggaa
     1501 gaaataggta aaaattcgct aactgcctgg gttaatccca aaatgagaga ttgaataaaa
     1561 gtcataggat attataaaca aaaacagcag cgatttagcg cttgggggat tgtttttgac
     1621 gtcgggcttt gccaccggta ccaaccatac gacgttcctt aacacgggaa tcaactgtca
     1681 gaaggccacc ggctctaaga accggtttga atgattcgtc aattttgatt aaggatctgg
     1741 caatggccaa gctgagagcc tgcacctgtc cggttttgcc cccaccgata atggtggcag
     1801 aaaaatgata tttaccagcg gttttggtga tttcaaaagg tttatcgtaa attatttttt
     1861 cggttttggt gggaaaatac ttgctaagtt gaataccgtt gactaaacaa tcacctttgc
     1921 cggaaaaaat tttgacggta gcgacagcac ttttgcggcg accgatagat cgagtataat
     1981 tggttttgga agtttttttt gccatattat ttagttaatt tgtcagcgta aggatgattg
     2041 ctgtcggtaa aaatttttaa acgggtcatt ctgcgatcgc gtaatttatt ttttggcagc
     2101 ataccataga cgccgctttt gatgaccaga cggggatctt ttttgagcat ctcatcaaga
     2161 gtcaattctt taagaccacc tggccaatgg gtgtgtcggc gatatatttt atcttttagt
     2221 ttggaaccgg tgactctgat atcggcggca ttgatagcta cgacgtagtc gccatcatcg
     2281 cgatggtaag aaaaggtggt tttgtctttg cccataagaa tagaagcaat ctgggtgcag
     2341 atccgaccta gagtttggtt tgataagtca atcagatgcc aattcctatt gacttgattg
     2401 ccttttagaa cactggtttt catgatttag tctcctgttt aactttcttt gatggtttag
     2461 ccttggttac ggccggtttt ttggtgtctt ttttatcttt gttgacgact tctttttcag
     2521 gcttgacggc gaatttaacg ggtttaacaa aactaagctt aacaatgaga gcatcatcac
     2581 catatctccg actaacccgg ctgatttggg taaaattttt ggtttggcca tcaaaaacct
     2641 gtttgaaggt ttgacaaaca ttgttgaccc aatttctgtc ctgaagatgg cggaacaatt
     2701 ctcttttggc aatcagttcg ggtttggtga taatagtatt ggacagcgat tcgatggtag
     2761 gaatgattga tttggccttg gcttcggtgg ttttgataga cccatgaata aacatagacc
     2821 gggtcaaaga ccggaacaaa gcctgacgtt cgtggtggtt tctacctagt tttttaccaa
     2881 aattacggtg tttcatatta acaaaaagtt taaattattt ccaagtaaag cccctttctt
     2941 ttagccagga atcaataatt ttgagcgatt tttcaccgac atttttggcc ttgctaactt
     3001 cggagcggcc agatttaact aaggtatcga tagtgctgta accggctttt ttgagagcat
     3061 tggtgacccg aagcggcaat tcaatttctt caaccgaaat atcgcttcct tgaggtgaaa
     3121 gagaagtatt gatggtaata atttcttctt caaaatcttt agggtcggca atttgcccaa
     3181 aagcgttgat aaggtctttg gcggccatct ttagggcata caaagcatca acagaaccat
     3241 cagtccaaat ttccatagtt aatttgtcat aattagactt tttacccaag cgggtaggtt
     3301 cgacggtata gttgcttttg agaatggggg taaaggcggc gtcaaggata atttcaccaa
     3361 taatatcggt tttttgctct ttggccatag tataaccaac tcccctttcg acggtcattt
     3421 cgatttttag actggatttg gcatcagata gggtggcaat aacctgatca gggttggcaa
     3481 cgcggcaaag atcgttatcg gtgatatcgc cggcagtaac aacgcccgga ccttttttgt
     3541 cgagtttaag aacaataggt tcttccttgg cataattaaa cttaatcttt ttgaggttta
     3601 gggaaatttc aaccaaatct tctttgacac cagggagagt ggtaaaaagg tgggtggcac
     3661 cgtcaacccg gattcgggtg atggctgcac cgggaatggt aatcaacaac acccggcgca
     3721 aactgttgcc gagagtgtga ccaaaaccgc caaccaatgg gctaagttca aacttaccgt
     3781 atttttcggt agatttgatg gttttgatat tgaacttatt agtttcaatc atggaattct
     3841 cctttttatc ttgaataata ttcaattatt aaattgatgt cgatgtcatt gccaatctct
     3901 tctttgagag gttcggaaat aactttgaca gtaaaatctt ttttgttgag gtcgagccaa
     3961 gacttggttt tgaaatcgga ttcatcagct ggaagagtgc cgatcttatc aataactttt
     4021 ttgtcaaggg aaataatatc accaactttt acctgatatg atggaatatt cataatttgg
     4081 ttgttaacca gggcatgacc atgaccgacg atttgcttgg ctgaagagcg ggataagcta
     4141 agaccggaca gatacaacac attatcgaga cgattttcta acagattgag caagttgtca
     4201 ccaactaaac ccctgagttt cttggcttta agataataat ttttgaattg aacttcctga
     4261 acgccataaa tccgtttagc tttttgcttg gcctttagct ggatgccgta atcggtgggt
     4321 ttgctggatg atttggcgcc atgcattccg ggtttgacag cgcctctttt ttcgatagga
     4381 catttggcgg aaaaacaacg ggcgccctta agatagagct tgtcaccagc tgcccggcaa
     4441 agtcgacatt tggcatctaa agtaattctt gacataaatt tgagttatta atttttaagc
     4501 ccgtcgctgt ttggg
//
LOCUS       DAOSHV010000042         4496 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_17389_length_4496_cov_4.623959, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000042 DAOSHV010000000
VERSION     DAOSHV010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 4496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4496
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17389_length_4496_cov_4.623959"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1115)
                     /locus_tag="PKZ29_02050"
     CDS             complement(<1..1115)
                     /locus_tag="PKZ29_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37682.1"
                     /translation="MKRVSLPVAITIGCGLIFLGLAASFLVKKGNLNRGNKPEPKETI
                     KRTDYTDVARKTLDWIDQQRNEDGWYILERGCDYEKKTCDIVWDNKEGNKDGLIATWA
                     RLNFFEQHQDPKDLKIIKKDIDLFYDKYKDDNLSDSLWLCKITYEMAQSKYIDQTQKD
                     KLKELCMGVEYPSTEEVKKYWLSERDKKVKEFAKANVWEKWDSYRLNIRGFDASLGYP
                     TDLIFRYKWTNNVDDLNAAKDTSDVLMKIYKDGIDKNFDKQIVRYPEDICLGIISAID
                     VYKLDKEQEMLEFASETYKQTVSNQEKLSESQTTVCGLTVKSFLDLTGDETYLSDLEK
                     NNKILINIGVDGENMIVVGDGGWFKSDGKNRFVTYKNV"
     gene            1338..1520
                     /locus_tag="PKZ29_02055"
     CDS             1338..1520
                     /locus_tag="PKZ29_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017954847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOG37683.1"
                     /translation="MDNLPDLLTVAEVADLLRVSKLTIKRWGKRGKLPAIRINARGDR
                     RYRKEAVLWLLGIQTE"
     gene            complement(1622..2611)
                     /gene="ruvB"
                     /locus_tag="PKZ29_02060"
     CDS             complement(1622..2611)
                     /gene="ruvB"
                     /locus_tag="PKZ29_02060"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003660790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOG37684.1"
                     /translation="MIKKIEENKTLDPKPEMAEKVVEKKLRPQRLNEFIGQEKIKEQL
                     EIFLTAAQSRGEQLDHILFYGPPGLGKTTLACLMAREMSCNIKITSGPALTRAGDLAS
                     ILTGLKKGDFLFIDEIHRLNKLVEEMLYAAMEDFALDIVLGKGPAAKTVRLNLQKFTL
                     VGATTRIGLISGPMRDRFGYVQQLDFYEDKDLGEIVERTAEVLGVKIEPQAAVSIAKR
                     SRGTPRIANRLLRRVRDYAEVKNDGLITLREVEEALKMLGVDDSGLSDADRRYLDIVK
                     NQYHGGPVGVENLAASLSEDVGTITEVYEPFLMKKGLIKRTPKGRVLGNEFVK"
     gene            complement(2677..3249)
                     /gene="ruvA"
                     /locus_tag="PKZ29_02065"
     CDS             complement(2677..3249)
                     /gene="ruvA"
                     /locus_tag="PKZ29_02065"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOG37685.1"
                     /translation="MISSLKGKINRMWGNNCEVEVGGVGYCIWVGRNFVKKHNQGEEV
                     TVSTYMAVSENEINLFGFENWEEVDLFKMLISVSGVGPKTAAGILGEAESEAVVRAIG
                     EADVDFFQKIKGIGKKTAQRIIIDLKSKIGGLGELDLTGDDKKTEPDEVFLSLKQLGF
                     ERKEIEKVIAKLPKEIIVIEEKIQWCLGNI"
     gene            complement(3409..4026)
                     /locus_tag="PKZ29_02070"
     CDS             complement(3409..4026)
                     /locus_tag="PKZ29_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37686.1"
                     /translation="MEAKNEVKPIEIGMSSVAEKIEMRKAQSSTDRLVEYMTGLRLDD
                     PELIDRLEIDQMKIRTKYGLPEREYRFANPGEYEKYLKEIAEVNGVKVRLKNDCGSFF
                     GKYAMAGGVYLNTEKTIGVDINKETKDDYTKSLATLEHELIHALQHKHYPGMPVEVQE
                     YEAYVAGWNIDYLRDKPESKEIVFGFGVMGSIKHWYTGIKKQVRR"
     gene            complement(4057..>4496)
                     /gene="ruvC"
                     /locus_tag="PKZ29_02075"
     CDS             complement(4057..>4496)
                     /gene="ruvC"
                     /locus_tag="PKZ29_02075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOG37687.1"
                     /translation="NIQESQAGEYKLVECGCLRTNIGEASARRLEKIYDELERLIKKY
                     KVEAMAIESLFFAKNAKSAIKVSEAIGVIKVCGQRNGIEVVEFTPLQVKTTLVGYGRA
                     QKDQVEIMVRNFLGLTGNISPSHASDAVAVGLTYLFTNKNLVV"
BASE COUNT         1187 a         1277 c          534 g         1498 t
ORIGIN      
        1 acatttttgt aagttacaaa tctgttttta ccatcagact taaaccaacc cccatcaccc
       61 accactatca tgttttctcc gtcaacacca atattaatca atattttatt atttttttct
      121 aagtcactca aatacgtttc atccccggtc aaatccaaaa acgacttaac tgtcaatcca
      181 cacacagtag tctgagattc tgatagtttt tcttgattgg ataccgtttg tttatacgtc
      241 tcactagcaa attccaacat ctcctgctct ttatctagtt tgtaaacatc tattgctgat
      301 attatcccca aacaaatatc ttccggatac ctaacaatct gtttatcaaa atttttatca
      361 atcccatctt tataaatttt catcaaaaca tcagaagtat cttttgctgc gttcaaatca
      421 tctacattgt tagtccactt atatcgaaat ataagatcag tcggataacc caaactggca
      481 tcaaaccctc ttatatttaa tctataacta tcccattttt cccacacatt agctttagca
      541 aactctttaa cttttttatc tctttctgag agccaatatt ttttaacctc ttcagtgcta
      601 ggatattcca ctcccataca taactccttc agcttatctt tctgtgtttg gtctatgtat
      661 ttgctttgtg ccatttcata agtgattttg cacagccaaa gtgaatcgga cagattatca
      721 tctttgtatt tgtcataaaa caaatcaatg tcttttttaa taatttttag atcttttgga
      781 tcttgatgtt gttcaaaaaa attcaatctg gcccaagtgg caatcagacc gtctttgtta
      841 ccctctttgt tgtcccaaac tatgtcgcaa gtttttttct cataatcaca tccccgctcc
      901 aaaatatacc aaccatcttc atttctctgt tggtcaatcc aatccagtgt tttccgagct
      961 acatcagtat aatcagtccg tttaattgtt tcttttggct ccggtttatt ccccctattc
     1021 aaattacctt ttttaaccaa aaaacttgcc gccaatccca aaaatatcaa gccacaacca
     1081 attgttattg ccaccggcaa gctgactctt ttcataaatt aatcatacct tatttcccca
     1141 cctcaaacca agtctatttt aaaatactaa ctccaaaata tacttacatc ttgacacaca
     1201 aacaaccgat atgatttact gaatacagtt aatcattatt ttgatacaag aggagccacc
     1261 acatggacta tcaaaccaac tcacaagaca aacttgtgaa acaaccatct gccgccaaaa
     1321 aagtcaatgg catttctatt gacaacctcc ccgatctttt aaccgtcgcc gaagtcgccg
     1381 acctactgcg ggtcagtaag ctcaccatca aacgctgggg caaacggggt aaacttcccg
     1441 ccatccgcat caacgcccgc ggcgaccgcc gctaccgcaa agaagccgtt ctctggcttt
     1501 tgggtattca aaccgagtaa ttaaaactcc cctgcccaat ttttttatct caaacaatct
     1561 gatttgatat ctgagatctg acaaactgac atctaattca ctttataaac tttataactt
     1621 tctactttac aaactcattt cccaacactc ttcccttcgg cgtccgcttt attagtcctt
     1681 ttttcatcaa aaatggttca taaacttcgg taatcgttcc cacatcctcc gacaggctgg
     1741 ccgccaaatt ctccactccc accggtcccc cgtgatactg atttttgact atatctaaat
     1801 accgtctatc tgcatcagac aaacccgaat catccacccc caacattttt agcgcttcct
     1861 ccacttccct caacgttatc aacccatcat tttttacctc ggcataatcc cgcacccgtc
     1921 tcagcaatcg gttggcaatc cgcggcgttc cccgcgatct tttggcaatc gaaaccgctg
     1981 cctgtggctc aatttttacc cccagtactt ctgccgtccg ctccacaatt tctcccaaat
     2041 ctttatcttc ataaaaatcc aactgctgaa cataaccaaa cctatctctc atcggtcccg
     2101 atatcaatcc aatcctggtg gtcgctccca ccaaagtaaa tttctgcaaa tttaaccgaa
     2161 ctgttttggc cgccggccct ttgcccagca ctatgtccaa agcaaaatcc tccatcgccg
     2221 catacagcat ttcctctact aatttattta gccgatgaat ttcatctata aacaagaaat
     2281 cccctttctt cagccctgtc agaatcgacg ccaaatcccc cgcccgggtc aatgccggtc
     2341 ccgaagtaat tttgatgttg cagctcattt cccgcgccat taaacaggct aaagtcgtct
     2401 ttcccaaacc cggcggacca taaaacaaaa tatggtccaa ctgctctcct cttgactgcg
     2461 ccgctgtcaa aaatatctcc aactgttcct ttattttctc ctgaccgata aattcattta
     2521 acctttgcgg tctcaatttt ttttccacca ctttttctgc catttccggc ttcggatcta
     2581 gtgttttatt ttcctctatt tttttaatca tattttattg ttttattttt atgtccaagt
     2641 atttttcctg atatctgacc aactgggatc tgacatctaa atatttccca ggcaccactg
     2701 tattttttct tcaataacaa ttatttcttt tggcaatttg gctattactt tttctatctc
     2761 ttttctctca aaccccagct gttttaagct caaaaacacc tcatctggct ctgtcttttt
     2821 gtcatctccc gtcaagtcca actctcccaa gccgccgatt tttgatttca aatcaataat
     2881 aattctttga gctgtctttt tgccaatccc ttttattttt tgaaaaaaat ctacatccgc
     2941 ctcgccaatc gccctgacca ctgcctcact ttccgcctcc cccaaaattc ctgccgccgt
     3001 ttttggtccc actcccgaca ccgaaatcag cattttaaac aaatccacct cctcccaatt
     3061 ttcaaatcca aacagattga tctcattttc tgacactgcc atataagtcg ataccgtcac
     3121 ttcctcgccc tgattgtgtt ttttgacaaa atttctcccc acccagatac aatatcccac
     3181 cccgcccact tccacttcgc agttatttcc ccacatccga tttatttttc ctttcaagct
     3241 ggaaatcata cttcatttta cactaacatg gcttcaatcc ccacgcccaa atcgacccgc
     3301 cttaactttt attttctgcc tcatatttat ctatttgttt ttgatcaatt ccgtcaacat
     3361 ttagcaaaaa atactccgga ttgtcccaca ccggcgtcaa tatttcgttt atctcctcac
     3421 ttgtttcttt ataccagtat accagtgttt tattgaaccc atgactccaa aaccaaacac
     3481 tatctccttt gattccggtt tatccctcaa ataatctata ttccagccag ccacataagc
     3541 ctcatattcc tgcacttcca ccggcatccc cggataatgt ttgtgctgta gcgcatgaat
     3601 taactcgtgc tccaatgttg ccagactttt ggtgtaatcg tctttagtct ccttgtttat
     3661 atctacacct atcgtctttt ctgtatttaa ataaactccg cccgccatcg catatttacc
     3721 aaaaaaacta ccacaatcat ttttcagtct taccttaact ccattaacct ctgctatttc
     3781 tttcaaatat ttttcatatt cacccgggtt agcaaaccga tattccctct ccggcaaacc
     3841 atatttagtc cgaattttca tctgatcaat ctccagtctg tctatcaact ccggatcatc
     3901 cagcctcaaa cccgtcatgt attccactaa tctatcagtg ctagactggg cttttctcat
     3961 ttctattttc tctgccaccg atgacatccc tatttctatt ggttttactt cattttttgc
     4021 ttccacaatt tactatccca cctttaccaa aatatatcaa accactaaat ttttgttggt
     4081 aaacaaatat gtcaacccca ccgccaccgc atccgaagcg tgggacggcg aaatatttcc
     4141 tgttaatccc aaaaaatttc tcaccattat ctccacctga tctttttgag cccggccata
     4201 gcccaccaac gttgttttta cctgcaacgg tgtaaactcc accacctcaa ttccatttct
     4261 ctgaccacaa accttaatta ctccaatcgc ctccgacacc tttatagctg atttggcatt
     4321 tttggcaaaa aacaggctct caattgccat tgcctccacc ttatattttt taatcaacct
     4381 ctccagctca tcataaattt tttccaatct tcttgccgac gcctcaccta tatttgtcct
     4441 caaacacccg cattctacca acttatattc acccgcctga gattcttgaa tattct
//
LOCUS       DAOSHV010000043         4468 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_17563_length_4468_cov_6.697938, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000043 DAOSHV010000000
VERSION     DAOSHV010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 4468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4468
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17563_length_4468_cov_6.697938"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="PKZ29_02080"
     CDS             complement(<1..521)
                     /locus_tag="PKZ29_02080"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HOG37688.1"
                     /translation="MKENNKGVMILVVVLILVFLLPWGRINWGKIRWMQPETVTVTGE
                     AKSTQKNQIATFNAGVDAVNSDKDKAISDVNSKMEAIIAAVKDFGIKEEDIKTQNLSI
                     FQQEETYYEGGVQKSRKGQWRISNGVEIVLREVDKASKLTSLLTSTGANNVYGPNFSF
                     DDVSETQKGLYEAA"
     gene            complement(595..1533)
                     /locus_tag="PKZ29_02085"
     CDS             complement(595..1533)
                     /locus_tag="PKZ29_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37689.1"
                     /translation="MIQERQIGFSPEKEEKFLLRLDGGCRRVNLEQELKTQLAEDFGL
                     VEFSLKFRLDEKGKIVDLLSGEEMVELTKRGGVEEEVESITKIEAGLGRGSESVWVHF
                     SPKNEELGYAGNCVDFWRREGKKVVWNRVAVKNNLAEMNELRNLLSGEKRVMTEWEIL
                     AQPVEVKGVSLGEIFDLLAISEVKNEVTKNQIDRIVENYTESFASELGEELVNNKDFI
                     FRLYSLCYRELEMNNTEMTIRGEVENYMYGSLMGVRTEASFGCSVTTIVGSFGEKVGY
                     YVLANGEVKRGQIPEGFKECKKCGCWYAGEKCPFCP"
     regulatory      complement(1593..1659)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(1709..2164)
                     /locus_tag="PKZ29_02090"
     CDS             complement(1709..2164)
                     /locus_tag="PKZ29_02090"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF014033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009235 - cobalamin metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP:cob(I)alamin adenosyltransferase"
                     /protein_id="HOG37690.1"
                     /translation="MITTKVGDGGKSWCGGKMVDKDGPLLEAMGSLDELQAGLMMVDE
                     KKIVKDLKEIMGIIASLSSSSSLSSCKVKDKVEWLEREIKKMDKGDVEEFLVFETKKA
                     REFNLVRTVCRRAERRLVGYGKVGKVDKGILIYINRLSDYLFLKAVLNN"
     gene            complement(2270..>4468)
                     /locus_tag="PKZ29_02095"
     CDS             complement(2270..>4468)
                     /locus_tag="PKZ29_02095"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HOG37691.1"
                     /translation="TPCLVNAGKARQQLSACFVLPIEDSMESILETMSNMAMIHKSGG
                     GTGFSFSNLRPSGDYIKSSGGTTVGPVSFMQAYNDVTAQIKQGGVRRGANMGMLNIYH
                     PDVLRFAVVKLDEWSLTNFNISLAVTNDFMKRMEEDKKFVTDDSIPEEAVEEIRLAEA
                     IRGVDDRLRETEKGIKKLYDWAEAKQEGEGYELINPRTNQVVMKLNAYKVFNLVTRLA
                     WQFGDPGLVFIDRMNEPSSNPVPTVGRIEATNPCGEQPLLPYDACNLGSVNLAKLVRD
                     KDIDWEELKRVVFEAVHFLDNVVEVNEFPVQQIRDMVSKTRRIGVGVMGFADALFKLG
                     VKYDSEEGIAWAKRIMKFVTESAKEASVELAKTRGVFPLWDVSVFANTDYKPRNMAIT
                     TIAPTGTISMLADTSSGIEPVFSLGFQKNTVEGKTLYSVNPVLVEELKNKGIYSEELM
                     EKIVKNGGTLGGVEEVPEEIKKVFVTALEIEPEWHIKVQAAFQEFTDNAVSKTINLPE
                     TATVEDVRKAYRLAYALGTKGITIYRTGSRSFEPLQKVKDKKDEAKDDLDYKPRKKPT
                     PIVAQGIRIKKKCDVGSIYSSIFYEENDGPVEVFVTLGKSGGYLAAAAEVTGRLSSLA
                     LKYGATLDEISEQLVGISCGQKVGMGNGAVLSMFDAVGKSLLEISRGQQLTMFEEKEI
                     PVKMDLVKPDNNEAEAESKFGACPDCGSPLRAEEGCFKCTNVFCGYSKCS"
BASE COUNT         1176 a         1340 c          513 g         1439 t
ORIGIN      
        1 gccgcttcat acaaaccttt ttgggtttca ctcacatcat caaaagaaaa attcggtcca
       61 taaacattat tcgccccggt cgaagtcaaa agtgatgtca gttttgacgc tttatccact
      121 tctcttaaca caatttctac cccattacta atccgccatt gtcccttccg gcttttttgc
      181 actccgccct cataataagt ctcttcttgt tgaaaaatgc tcaaattctg cgtcttaata
      241 tcttcctctt ttatcccaaa atctttaact gccgcaataa ttgcctccat tttgctatta
      301 acatcactta tggctttatc tttatccgaa ttgactgcat ccactccggc attaaaagtc
      361 gctatttgat ttttttgggt tgatttagcc tcgccagtca ccgtcactgt ctccggctgc
      421 atccatctaa tctttcccca atttattctc ccccaaggca acaaaaacac taatattaac
      481 actactacca gtatcattac acctttatta ttttctttca taccttaatt atcccttatt
      541 tttaatttaa ataaatatat caaaaaacat caagtaatat gtcaaatcac atcatcaagg
      601 gcaaaaagga catttctctc cggcatacca acagccacat tttttacact ctttaaatcc
      661 ctccggaatc tggcctcttt taacctcgcc attggccaaa acataataac caaccttctc
      721 tccaaacgac cccactatgg ttgttaccga gcagccaaaa ctggcttcag ttctcacccc
      781 catcaaactg ccatacatat aattttctac ctcccctctt attgtcattt cagtattgtt
      841 catttccaac tcccggtaac acagcgaata tagccgaaaa ataaaatctt tattgttcac
      901 taattcctct cccaattcac ttgcaaaact ctcggtataa ttttctacta tccgatcaat
      961 ttggtttttt gtcacctcat ttttaacctc acttatggcc agcaaatcaa aaatctctcc
     1021 caaactcact ccttttactt ccaccggctg cgccagtatc tcccactctg tcatcactct
     1081 tttctctccc gacaataaat ttctcaactc attcatttct gccaaattat ttttcaccgc
     1141 cacccgattc caaaccacct ttttcccctc ccgtctccaa aaatcaacac agttgccagc
     1201 atatcccaat tcctcatttt taggactaaa atgaacccac accgactctg accctctccc
     1261 taaaccagcc tcaatttttg ttatcgactc aacttcttcc tcaaccccac cccttttggt
     1321 caactccacc atctcctccc ccgacaacaa gtccacaatt ttccccttct catccaatct
     1381 aaatttcaag ctaaattcca ctaacccaaa atcttccgct aactgagttt ttaactcctg
     1441 ctccaaattt actctccgac aaccaccatc caatcttaac aaaaattttt cttctttttc
     1501 tggcgaaaag ccaatttgtc tttcttgtat catatttact cttagttttg ttacaaacta
     1561 atcccgaaac ttcgggatgg cgcgaaagtt ttggtcttct gacttagaga ttactctcat
     1621 cacagttgcg gcccagtgta ggaatcaaac ctacttcccc cggcattatc tgccgccccg
     1681 acttaattta ccagtcggag tttaattttt aattatttaa aactgctttc aaaaacaaat
     1741 aatctgacag tctattaata taaatcaaaa ttcctttatc aaccttaccc accttaccat
     1801 accccactaa tctcctctcc gcccgccggc aaacggttct gaccaaatta aactctcttg
     1861 cctttttagt ttcaaaaacc aaaaattctt ccacatctcc cttatccatt tttttaatct
     1921 ctctttccaa ccactcaacc ttatccttta ctttacaact tgataaactt gatgaacttg
     1981 acaaactagc aataattccc ataatttcct tcaaatcctt gactatcttt ttctcgtcca
     2041 ccatcatcaa tcccgcctga agttcatcca aactccccat cgcctccaac aacggaccgt
     2101 ctttgtccac cattttcccc ccacaccagc ttttgcctcc atcacctact tttgttgtaa
     2161 tcatgttaat ttattactta aattttttat attttttatt cttaaatcct aaaattattt
     2221 taaatatttt gtttttatta ctttaaaact ttaaaaactt gacaaacttt taactacatt
     2281 tagagtagcc acaaaacaca ttggtgcatt taaaacaacc ctcttcggct ctcaacggcg
     2341 aaccgcagtc cggacaagcc ccaaatttac tctccgcctc cgcctcatta ttatctggtt
     2401 taaccaaatc cattttcacc ggtatctctt tttcttcaaa catcgtcaac tgttgccctc
     2461 gggaaatctc caacaacgat ttccccaccg catcaaacat gcttaacact gccccatttc
     2521 ccatccccac cttttgaccg caggaaattc ccaccaactg ctctgagatc tcatccaagg
     2581 tcgctccgta ttttaacgcc agtgacgaca atcttcccgt tacttctgct gctgccgcca
     2641 aatatccccc acttttcccc aaagtcacaa acacctctac cggaccatca ttttcttcat
     2701 aaaaaattga tgaataaata ctgcccacat cacacttttt cttaatcctg attccttgag
     2761 ccactatcgg cgttggtttt ttcctcggtt tataatccaa atcatccttc gcttcatcct
     2821 ttttgtcttt cactttttgc agcggctcaa atgatcttga tcctgtccga taaatggtaa
     2881 tccccttagt tcccaaagcg taagccaatc tataagcttt ccgcacatct tctactgtag
     2941 cagtttccgg caaattaatt gtttttgaca ccgcgttgtc ggtaaactcc tgaaatgctg
     3001 cctgaacttt gatgtgccac tccggttcaa tctccaaagc ggttacaaac acctttttta
     3061 tttcctccgg cacttcctcc actccgccaa gcgttcctcc gttttttaca attttctcca
     3121 tcaactcctc gctataaatt cctttgtttt ttaactcttc taccaatacc ggattaacgc
     3181 tataaagcgt ctttccttcc actgtatttt tttgaaatcc caacgaaaat accggctcaa
     3241 ttcccgaact cgtatctgcc aacatactga ttgtccccgt cggcgcaatc gtggtgatgg
     3301 ccatatttct cggcttataa tccgtgttag caaacacact cacgtcccac aaaggaaaca
     3361 ctcctctggt tttagccaat tccaccgacg cttccttggc tgactccgtt acaaacttca
     3421 ttattctttt tgcccaggca attccttcct ccgaatcata tttcactccc aatttaaaca
     3481 aagcatccgc aaatcccatc actcccaccc caattctcct ggtcttgctc accatgtctc
     3541 tgatttgttg caccgggaat tcattaacct ccaccacatt atccaaaaaa tgcaccgcct
     3601 caaatactac ccgcttcagc tcctcccaat caatatcctt atctctaact aatttggcta
     3661 aattaacgct tcccaagtta caagcatcat aaggcaacaa tggctgttcc ccgcaagggt
     3721 tggttgcctc aatccgaccg actgtcggca ccggatttga actcggttca ttcatccgat
     3781 caataaacac caaccccgga tcgccaaact gccacgccaa tctggtcact aaattgaata
     3841 ctttgtaagc attcaatttc atcaccacct ggttagttct gggattaatc agctcatatc
     3901 cttccccctc ctgttttgcc tcagcccaat catataattt cttaattcct ttttctgttt
     3961 ctctcaatct gtcatccacc cctctaattg cctccgccag tctaatttct tccactgctt
     4021 cttccggaat tgaatcgtct gtcacaaatt ttttatcctc ttccatccgt ttcataaagt
     4081 catttgtcac cgccaaggaa atattaaaat tagtcaaact ccattcatcc aatttcacca
     4141 ccgcaaacct caacacatcc ggatggtaaa tattcaacat tcccatatta gctccccgtc
     4201 tcactcctcc ttgttttatc tgcgccgtca catcgttgta agcctgcata aaactcaccg
     4261 gtcccaccgt cgtcccaccg ctcgatttaa tataatcgcc gctcggtctt aggttagaaa
     4321 aagaaaatcc tgttcctccg ccgcttttgt gaatcatcgc catatttgac attgtttcca
     4381 aaattgattc catcgaatcc tctatcggca gcacaaaaca ggccgacaac tgctgtctgg
     4441 ctttgcctgc atttaccaaa cacggcgt
//
LOCUS       DAOSHV010000044         4452 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_17659_length_4452_cov_6.099159, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000044 DAOSHV010000000
VERSION     DAOSHV010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 4452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4452
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17659_length_4452_cov_6.099159"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            569..808
                     /locus_tag="PKZ29_02100"
     CDS             569..808
                     /locus_tag="PKZ29_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37692.1"
                     /translation="MNNKKIHRVMTRAVAEKYNPLEQIALSEAFQLLIRSFPQERHFI
                     IGFAYANEGQGEKVWLIIDESDTERIETMLFPDDY"
     gene            complement(1379..2158)
                     /locus_tag="PKZ29_02105"
     CDS             complement(1379..2158)
                     /locus_tag="PKZ29_02105"
                     /inference="COORDINATES: protein motif:HMM:NF017843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HOG37693.1"
                     /translation="MSLLMHRGGEYVTKEQLDLIPVPEATESYVPVSHYHLTDKFLTI
                     SQDILRDYVLIGQNYGIARQGQQLFAVLKFQNEKSEMGLSIAFRNSYDRSMAIGLAIG
                     ASVFVCDNLALSGDVTVMKKHTKNVWEDLEETAIATIYKSQKNYTQIVCDAEAFKTLP
                     MGNNAAFRTMGLLFGTNIISPRQLTILKDEWLKPSYKEFESRNLWSFYNAATESLKSS
                     PPVTIMEKHIRLHETLIQIGKEASHVQNGTNTAGLLPGGER"
     gene            complement(2155..2382)
                     /locus_tag="PKZ29_02110"
     CDS             complement(2155..2382)
                     /locus_tag="PKZ29_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37694.1"
                     /translation="MKYIVTVYLRYQEQIIDKFILRNITQEELNSGLDGFEAKFKELC
                     GNFPKLPEDVTYILEVKPQKRRTLKKKGEEQ"
     gene            2650..3588
                     /locus_tag="PKZ29_02115"
     CDS             2650..3588
                     /locus_tag="PKZ29_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOG37695.1"
                     /translation="MNKKLSIGFQRSCQCDPTHINCLTAKEWLKCQLGVWQFHFEGRD
                     IRDKSVHPATFPISLARKVIELFSHKGELVLDPFVGSGTVLIASKDSDRNAVGFDLQD
                     NYIKLCESRLKQTALFGNTEQIAIKEDAHNIKKYLDKESVSLIFTSPPYANLLNRERK
                     NKSRRDRKNDQLGKVEQYSQDPRDLGTMPLEEYSTAMGDIFEDLLPILKPKAHCVINV
                     PDMWWENERITIHVSLIQELRKRGYELRNIIIWDRTNIVNKIGIFGWPSNYITMGVTF
                     EYLLDFWRPPNNEALHKGRANKRDKTDIQEGVDSEC"
     gene            3512..4318
                     /locus_tag="PKZ29_02120"
     CDS             3512..4318
                     /locus_tag="PKZ29_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011362423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MvaI/BcnI family restriction endonuclease"
                     /protein_id="HOG37696.1"
                     /translation="MKLCTKEELISEIKQIFKKGWIPSVKNTTDTRNDGAVGNTLEVL
                     LGIEENNLPIPNANEWELKGQRAHTSSLITLKHIEPSPQAAKIVANILLPKYGWPHKE
                     AGKKYPRGEKSFRSTTGATEFTKRGFKIVVDRKENKVKFVFDSYKADIKDNEVIFWLN
                     SVKERVGLGKLEPEPYWGFNDLERTIGEKAKNCFYVIADTRIESRKEYFKYEKLFILS
                     GFSFNNFLKCIEDGSVLIDFDARTGHNHGTKFRLRQDSIPKLYSKIDKVI"
BASE COUNT         1373 a          875 c          918 g         1286 t
ORIGIN      
        1 aatcattttt caattcataa ccgtaggttt ctgtacgctc gctcccgctg gtccataatg
       61 ttttaaaacc gtttctatgc ttaccttagc ttttatctct ttgtaatcaa cccaattttc
      121 ttttgccata ggcaccccct tattatttat atctaaatag ttataaataa taagaaaaat
      181 gtcaaattgc ccctttgccc ataaagtggg caatttgcta tttctaaccc tggtttggct
      241 atctatgcca tttattccac agataaaggg aaggttataa acaaaggttg ttcataaccg
      301 ataaacccca ctccaacaac cacaaaaccc ccgcccccga aatgctgcgc cttaaaccca
      361 cgggcaagac aaaacgtccg ctgtccttgc cgtcttccat tgtagcaagg aatcccccag
      421 ggctatcaag ctgattcttg cagacgtcca gcttgacagc cctggtgccc ccttgcttga
      481 ccaacgggcg aggcaaggac agctcaaagc ctctgtcagc cttaagtaaa ggtcgaaagg
      541 cggcactaac aaactaaact tcaaccatat gaacaacaag aaaatccatc gagtgatgac
      601 gcgggccgtt gcggaaaaat acaacccgtt agagcaaatc gccctatccg aggcctttca
      661 gctcctaata aggtcatttc cgcaggaaag gcacttcatc atcggttttg cctatgccaa
      721 cgaaggccag ggcgagaaag tttggctcat catcgacgag tcagacacgg aacgcatcga
      781 gacaatgctt ttccccgacg attactaatt aattcttaaa gccaaaccta tgaaaaaaac
      841 agaaagcaaa aggtcaggct acctctcaca ggttatcgaa gagataaata tcctggttga
      901 cgaggcagaa ggccgagacg agcttaagga acgactgagt tcctacatac cggacaaaat
      961 attacagtcc tacaaaaacg gaatagcagc agggcggaaa cggacagccc gggaatagcc
     1021 gggaaagggg cggggcaact cgcccctttt tttatgcttc tgaaagccat ttcggggggc
     1081 gttcccaccc accccaagga agataacatt aattgaaggc gtggttgttg gagtggggtt
     1141 tatttcaagg aaatatcgaa gaaatgaaac acccttgact aaatcaaggg tgtagaacgc
     1201 ttacctccgg caagctggtt aatcgtatca ggaatgacaa agggttttaa aaccgccgaa
     1261 gctgataact attaaggtgt ttgaaagatt aacgccggga ctcgcatagt gtccgcaatc
     1321 taactttatc cgcttgtctt tgagaaattc tcggagctcg taagaagtga tttgcttttt
     1381 acctttcacc accgggcaga agtccagcgg tatttgttcc attttgaaca tggctggcct
     1441 cctttccgat ctgaatcagg gtttcgtgta atctgatatg tttttccatt attgttactg
     1501 gcgggcttga tttgaggctt tctgtggccg cgttatagaa ggaccaaagg tttcttgact
     1561 caaactcttt gtacgatggt tttagccatt catcttttaa gatggttaat tgccgtgggc
     1621 tgatgatatt agtgccgaac aaaagtccca tggttctaaa tgctgcatta tttcccattg
     1681 gcaaagtttt gaatgcctcg gcatcacaaa caatctgtgt gtaatttttt tggctcttat
     1741 aaattgttgc gatggccgtt tcttccaggt cttcccagac gtttttggtg tgttttttca
     1801 tcacggtgac atcgccgcta agggcaagat tatcacagac gaatacagac gccccgattg
     1861 ccaagccgat tgccattgac cgatcatagg aattacggaa ggcaatcgaa aggcccattt
     1921 cagatttttc gttctggaat ttaaggacag cgaaaagctg ctgtccctgc cgggcaatgc
     1981 cgtagttttg gccgatcagg acataatccc tcaatatgtc ttggctgata gttagaaatt
     2041 tatcggttag gtgataatgg gatactggga catagctttc ggtcgcttcc ggcaccggta
     2101 taaggtcaag ttgttctttg gttacatatt ccccgcccct gtgcattaat aagctcattg
     2161 ttcctctcct ttctttttta aagtgcgtct tttctgtggt ttaacctcca agatataggt
     2221 tacatcttcg ggcaatttcg ggaaattacc gcatagctcc ttgaattttg cctcaaatcc
     2281 gtctaatcca ctattaagtt cttcctgggt aatattacga aggataaact tgtcgataat
     2341 ctgctcttgg tagcgaagat aaacggttac aatgtatttc atgattgctt tcccttctat
     2401 gtatataggt ttcgatcttt gatttattct tcaaacacca tactatgtat tcaatattat
     2461 atatcttttg gcatatttgg atatttgtca agggctgaga agaacaagtc ccaattttgc
     2521 aatttagagc caatcttttt tttaaaaaag atttaaataa gttttttggc tttttacctc
     2581 tagagttgtt tttataattt atcttgatat ttttagagaa tccaatgtaa aggctctgcg
     2641 gaggatttaa tgaataaaaa gctaagtatc ggatttcaga ggtcttgcca atgcgatcct
     2701 actcatatca actgtcttac cgccaaagaa tggctgaaat gccaactagg ggtatggcag
     2761 tttcatttcg agggtaggga tattagggat aaaagtgtcc atcctgctac tttcccaatt
     2821 tccttggcta gaaaggttat agaacttttt tcacataagg gagagcttgt attagatcct
     2881 tttgtgggta gcggaaccgt tttaattgcc tctaaggaca gtgacagaaa tgcagtcggt
     2941 tttgatttgc aggataatta tattaagttg tgtgaaagtc gtctcaaaca aaccgcattg
     3001 tttgggaata cagaacaaat tgcaattaaa gaagatgccc ataatattaa aaaatattta
     3061 gataaagaaa gcgtcagtct tattttcaca tcaccgccct acgctaacct tttaaacaga
     3121 gagagaaaaa ataaatctcg cagggataga aaaaacgacc aattaggaaa ggttgagcag
     3181 tattcacaag acccgcgtga tctgggaact atgcctcttg aagaatactc aacggcaatg
     3241 ggtgacattt ttgaggacct tttgcctatt ttaaaaccaa aggcccattg tgtcatcaat
     3301 gttcctgaca tgtggtggga aaacgaaaga ataaccatac atgtctcttt aatccaagaa
     3361 ttaagaaaac gaggctatga attaaggaac attattattt gggatagaac aaatatcgtc
     3421 aataaaatag gtatctttgg ttggccgagt aattatatta caatgggagt tacatttgaa
     3481 tatttattag acttttggag accgccgaac aatgaagctt tgcacaaagg aagagctaat
     3541 aagcgagata aaacagatat tcaagaaggg gtggattccg agtgttaaaa acaccactga
     3601 cacaagaaat gacggagcgg taggaaatac tttagaggtt ttacttggaa tagaagaaaa
     3661 taatttaccc attccgaatg ctaatgaatg ggaattgaaa ggccagagag cgcatacaag
     3721 ctctttgatt accttaaagc acatagaacc atcaccacag gcagccaaaa tagtagcaaa
     3781 tattcttttg ccaaaatatg gttggcctca caaagaagct ggtaaaaaat atcccagagg
     3841 agaaaagagt tttcgctcaa caaccggtgc cactgaattt acaaaaaggg gattcaaaat
     3901 tgtagtcgac agaaaagaaa acaaagtcaa atttgtattt gattcatata aagccgatat
     3961 aaaagacaat gaggtaatat tttggcttaa ttctgttaaa gaacgggttg gacttggaaa
     4021 gttagagcca gagccatatt ggggctttaa tgatttagaa aggacaatcg gagagaaagc
     4081 aaaaaattgt ttttatgtga ttgcagacac aaggattgaa agcagaaaag aatattttaa
     4141 atatgagaaa ctttttattt tatccggatt ttcttttaat aactttttaa agtgtatcga
     4201 agatggatcg gtcttgatag atttcgacgc cagaactggt cataaccacg gaactaaatt
     4261 tagattaaga caagacagca ttccaaaact ttacagtaag attgacaaag tcatttaaaa
     4321 gagccatttt actcaaaatc ttccgcttta tctcaaaccg gtaagttata ttcgcgtacc
     4381 ggcaagaaca ttgttgtgcg gataaaacat ttgatttatc ctctaaaatt atttataata
     4441 aaaaccctta at
//
LOCUS       DAOSHV010000045         4284 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_18594_length_4284_cov_5.066210, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000045 DAOSHV010000000
VERSION     DAOSHV010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 4284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4284
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18594_length_4284_cov_5.066210"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            78..1214
                     /locus_tag="PKZ29_02125"
     CDS             78..1214
                     /locus_tag="PKZ29_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37697.1"
                     /translation="MYFTKYNRHFKLIALTLASTILFWLIFYFNIPGKIGFDNVSLET
                     IFANYDGPNYMVISKCGYDKNCIGPNFSLPQPLEYYPAHFPGYPALITFFSNFTTGPK
                     AMIFSTLLGSVFLSLILYEFLKLYLKEKKSFWLTLVFLFFPARFFVLRQVGAPETWFL
                     AATIASIFYYKKEKFWLSAIFAALSMAFKTPAILLLASYGIVAIKDWIDIRKFLPVFK
                     KYFPYILVPITVVLIFYLYYLQTGNFWAYFHSGDNFHLNFLPYLVFVSTKSWINTIWL
                     EDIVYIFLLAIYGVYRLVKKYKFDITAVYPLVFTLATLLVAHRDISRYIAPVYPFMLL
                     AYKKFFTKHPVKIILILLIPAIVLYAINFVWGNTAPISDWTNYL"
     gene            complement(1211..2089)
                     /gene="dprA"
                     /locus_tag="PKZ29_02130"
     CDS             complement(1211..2089)
                     /gene="dprA"
                     /locus_tag="PKZ29_02130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOG37698.1"
                     /translation="MNEWSKWPIKLIDKKDFPPQLGEIRLCPEKLYYRGEWKSELFDK
                     VVAVVGSRQITRYGRDVVDKIMPEIVFRKATVISGFMYGVDSESHRQCVEYGGKTVAV
                     LGSGLDRPYPKENSRLYTEILNSGGVVVSEYEPDFGATVWSFPQRNRIVAGLVNVGVV
                     IIEAGIKSGSLITAKLGLEQKKKILAIPGPINSKTSEGTNWLIKTGAAKMVTEPVDIF
                     EDKIAMPDQENLFKDYSNLTDLERNIIEVLENEALNSDEICQKIKKPINEVSTTLSMM
                     LMKDLLTEEDGKFFIS"
     gene            complement(2082..2465)
                     /locus_tag="PKZ29_02135"
     CDS             complement(2082..2465)
                     /locus_tag="PKZ29_02135"
                     /inference="COORDINATES: protein motif:HMM:NF009150.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOG37699.1"
                     /translation="MMNNKVRNYKTGKEGEEIARKFLVQKGFRLIESNYSNKLGEIDL
                     IMLDKEVLVFVEVKLKIGDKFGSPEEMISKYKLSQIRRVAETFLVLNPDISKRLVKYR
                     IDAVCIVLNEDKTINRINYYENVYE"
     gene            complement(2455..3993)
                     /locus_tag="PKZ29_02140"
     CDS             complement(2455..3993)
                     /locus_tag="PKZ29_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014062918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOG37700.1"
                     /translation="MLTKVLSIANVGLQGVEIEVETNVAEKGFPGFGIVGLASKAVEE
                     AKERVKTAIINSEIEFPNAKVTINLAPADLPKDGSCYDLPISVGVLSSIREMTMPKEK
                     AYFYGELSLDGSLRHTKGVFLLALLAKEKGIKKIFVPRMSANEASVIDGVEVYPVDNL
                     KNLIKHLSGEKLITPLKKVETEEILDDIRPEFDFAEILGQEQAKRALEIAAAGGHNVF
                     MMGPPGSGKTMLARAMSGILPNLTEKESLEVTKIYSITGNIPPGGSLIRQRPFRSPHH
                     TTSQVGLIGGGSNPRPGEVSLAHRGVLFLDEFPEFGRNSLEALRQPLEDGLVNISRAA
                     GSVTFPAQFMLVAAANPCPCGYLGDPKKDCKCSSFQILNYRKKLSGPIMDRIDIHLTV
                     PAVDVNHLMITKDEINNNEKSEAIKQRVIEARKIQNQRFKNLAHIYSNSDMKNRHLKE
                     FANLNPEANLLLKQAVNKFNLSARVYFRLIKVARTIADLEKSEEIKQNHIAEALQYRI
                     KNDE"
     gene            4052..>4284
                     /locus_tag="PKZ29_02145"
     CDS             4052..>4284
                     /locus_tag="PKZ29_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37701.1"
                     /translation="MDIIDWFKNATLVVKIITGVLVLTILLLLLSLFTGGKSKTNVAT
                     TNTGGKDFEEIQIETPTPAPTTIIPTPIPTSSHT"
BASE COUNT         1111 a         1041 c          643 g         1489 t
ORIGIN      
        1 gaaccccggt tagcggaatg agaatccgct gtcctaaccg ctagacgata gggcctaatg
       61 ttgtaaaatt agctaagatg tattttacca aatataaccg tcactttaag cttatagctt
      121 tgactttagc ctccactatt ttattctggt tgatctttta ttttaatatt cccggcaaaa
      181 ttggttttga caacgtttct ttagagacca tttttgccaa ctacgacggt cctaattaca
      241 tggttatttc caaatgcggc tatgacaaaa actgcattgg tcccaatttt tccctgcccc
      301 agcctctgga atactacccg gctcatttcc ccggttatcc ggctttaatt acatttttta
      361 gtaacttcac caccggtccc aaagccatga tcttttccac tcttttggga tcagtttttc
      421 tcagtctcat tctttacgaa tttctcaaac tttatcttaa agaaaaaaaa tctttttggc
      481 tgactctagt ttttttgttt tttcccgccc ggttttttgt tcttcgccag gtcggcgctc
      541 ccgaaacttg gtttttggcc gccactatag cttcaatttt ttactacaag aaagaaaagt
      601 tttggctgtc agccattttt gctgctctat ccatggcttt caaaacccct gccattcttt
      661 tgttagcttc atacggaatt gtggctatca aagattggat tgatatccgg aaatttttgc
      721 cggtattcaa aaaatatttt ccctatattt tagtgcctat caccgttgtt ttgatttttt
      781 atctttatta tctccagaca ggaaattttt gggcctattt ccatagtggc gacaatttcc
      841 atcttaattt tttaccttat ttggtttttg tcagcactaa atcctggatt aacaccattt
      901 ggttggagga tattgtttat atctttttgt tggctattta tggagtttac cgcttagtca
      961 aaaaatataa atttgatatt actgccgttt atcctttagt ttttaccctc gccactttgc
     1021 tggtagctca ccgcgatatc agtcgttata tcgccccggt ttatcctttt atgcttttgg
     1081 cttacaaaaa attttttacc aaacatcccg tcaaaattat tcttattctc cttatccccg
     1141 ccatagtttt atacgccatc aattttgtct ggggcaatac cgctccaatc tcagattgga
     1201 ctaactacct ttaggaaata aaaaattttc cgtcttcttc tgtcagcaaa tctttcatca
     1261 acatcattga taaagttgtt gaaacttcat taatcggttt tttaattttt tgacaaatct
     1321 catcactgtt caatgcttcg ttttccaaca cctcgataat atttctttcc aaatcagtca
     1381 gattactgta atccttaaac aagttttcct gatccggcat agcaatttta tcttcaaaaa
     1441 tatctaccgg ctctgttacc atcttggcgg ccccggtttt gatcagccag tttgttcctt
     1501 ccgacgtttt tgagtttatc ggtccgggga ttgccaaaat ctttttcttt tgctccaacc
     1561 ccaatttggc cgtaatcaaa ctgccagatt ttatccccgc ctcaataatc accactccca
     1621 cgtttaccaa cccggctaca attctattcc tttgaggaaa actccatacc gtcgctccaa
     1681 aatcgggttc atattccgat actaccaccc cgccgctatt taatatttcc gtataaagtc
     1741 tactgttctc tttggggtaa ggtcggtcaa gaccactgcc caaaaccgcc acagtttttc
     1801 ctccatattc gacacactga cggtgggact cgctatcaac tccatacatg aaaccggaaa
     1861 tcaccgttgc ttttcgaaac acaatttccg gcatgatttt gtcaaccacg tcgcggccat
     1921 atcgggtaat ctggcggctg ccgactactg ccaccacctt atcaaacaat tcacttttcc
     1981 actcgccccg ataataaagc ttttccggac acaatctgat ttctccaagc tgtgggggaa
     2041 aatctttttt gtcaatcagt tttatcggcc attttgacca ttcattcata aacattttcg
     2101 taataattaa ttcgattaat agttttatct tcgtttaaaa ctatacacac agcatcaatc
     2161 ctgtatttca ccagacgctt gcttatgtcc ggatttaata ccaaaaatgt ctctgccact
     2221 cgcctgattt gactaagttt atattttgaa atcatctcct ccggcgaccc gaatttatca
     2281 ccaattttta attttacttc cacaaacacc agcacctctt tatccaacat aatcaaatct
     2341 atctctccta acttattact atagttagat tcgattaacc taaacccttt ttgaaccaaa
     2401 aattttctgg caatttcctc tccctcctta cctgttttgt aatttctgac tttattattc
     2461 atcatttttt atccggtatt gcagagcttc ggcaatatga ttttgtttaa tttcttcgct
     2521 tttttccaaa tcagcaatcg ttctagctac cttaattaat ctaaaataaa ctctggcaga
     2581 cagattaaat ttgttcaccg cctgttttag tagcaaatta gcctccggat ttaaattagc
     2641 aaactctttt aggtggcggt ttttcatatc cgaattacta taaatatgag ccaaattttt
     2701 aaatctttgg ttttgaattt ttctggcctc tatcacccgc tgtttaattg cttccgattt
     2761 ttcattatta ttaatttcat ctttggttat cattaaatga tttacatcta ccgccggtac
     2821 tgtcagatga atatcaatcc ggtccataat cggccccgac agtttttttc gataattcaa
     2881 aatttgaaaa ctgctgcatt tacagtcttt ttttgggtca cctaggtagc cgcaggggca
     2941 gggattagct gctgccacca acataaactg agccggaaaa gtaaccgacc cggctgctcg
     3001 ggagatatta actaatccgt cttccagggg ttgcctcagc gcctccagag aatttcttcc
     3061 aaattcggga aattcgtcca aaaacagcac tccccgatga gctaacgata cttcacctgg
     3121 tcggggattg ctgcctccac caatcaaacc cacctgtgag gttgtatgat gaggcgaccg
     3181 aaacggcctt tgccggatta atgatccacc cggcggaata ttgccggtaa ttgaataaat
     3241 tttagtcacc tccaaacttt ctttttctgt caaatttggc aatattcccg acatggctct
     3301 ggctaacatg gttttaccag atccaggcgg ccccatcata aacacattat gaccaccggc
     3361 tgcggcaatt tctaacgctc ttttggcctg ttcttgaccc aaaatttctg caaaatcaaa
     3421 ctccggtctg atatcatcca aaatttcttc agtctccact ttctttaacg gtgtaatcag
     3481 cttttctccg ctcaagtgtt ttatcaaatt ttttaaatta tctaccggat atacttcgac
     3541 tccatcaatc acgctggcct cattggcgct cattctgggg acaaatattt ttttaatccc
     3601 tttttctttt gccaatagtg ccaacaaaaa tactccctta gtatgtctta aacttccatc
     3661 cagagacaat tcgccataga aataagcttt ttcctttggc attgtcattt ccctgataga
     3721 cgacaacact cccaccgata tcggcaaatc atagcaagat ccatcttttg gtaaatccgc
     3781 tggcgctaaa tttatagtta ccttagcgtt gggaaattca atttcagaat taattatggc
     3841 tgttttgact ctttctttag cttcttctac cgctttgctt gccaacccca caataccaaa
     3901 cccagggaac cccttttccg ctacattagt ctcaacttct atttctacac cctgcaaacc
     3961 cacattggca attgacaaaa ctttagtcag cattataaaa atttaacaca aagttgtata
     4021 attttttcag tcttttgtta aactcttata tatggacatc atcgattggt ttaaaaatgc
     4081 gactttagta gtcaaaataa tcactggagt tttagtactc actattctcc tcctgctttt
     4141 aagtcttttt actggtggca aatctaaaac caatgttgct accaccaaca ctggtggtaa
     4201 agattttgaa gaaattcaaa ttgaaacccc gactccagcc cccaccacta ttattcctac
     4261 tcctattccc acttcttccc acac
//
LOCUS       DAOSHV010000046         4180 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_19231_length_4180_cov_4.777697, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000046 DAOSHV010000000
VERSION     DAOSHV010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 4180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4180
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19231_length_4180_cov_4.777697"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1987
                     /locus_tag="PKZ29_02150"
     CDS             <1..1987
                     /locus_tag="PKZ29_02150"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOG37702.1"
                     /translation="LQYKLSMKRLDQIIRFLYLSLFFLTPLIFNFRNSELFELPKMHF
                     VYFLTIFIVSLHFINWLSGQTKLVVKHPFSFPLLLFLISQIITTFTSIDPFTSFFGYY
                     SRLNGGLVSIITFTSLFYCLIPYLTPRFQKNIINISLFSGFLVAVYGILQHFGIDRHL
                     WVQDVQNRVFSSLGQPNWLAAYLCLILPLSLYQSHQTQNPKISLLHHLNSLALFACLL
                     FTKSKSGLIAAAVSLGIYLLYQLISDFKHHTFPKNLKSYLIFILFFIITLTTKNPLTQ
                     KFFTSPPSPQPTNSPANLIITPSQDIRRIVWRGSLDLWQKFPLFGTGVETFAYSYYWT
                     RPIEHNLTSEWDFLYNKAHNEYINYLATTGLIGFIPYIFLIITFLVVLFKNRHSKLFL
                     PFLTSYLSILITNAAGFSVVVISLYFFLFPALFLLPATKSPTPPPKHKYLMVIPLIVG
                     LCLTYLNLTSYLADIYYAKSESADNRQNYPDAYKYINLSLKLNPYQPTYLIYHALASA
                     KMAVATQDQSYLQPAITSANRSVAISPANLNFWRQRAQVYYYLSGFGSDYFIKTVESM
                     IQASKLAPTDPKNYYSLAQFLESAQLTDDAIFYYQQAIKLKPNYDHAYFSLGKIYFDK
                     KDYHPAADNLQQAVNYSHPVNTEAQKLLDQIKTSSKP"
     gene            complement(1984..2535)
                     /locus_tag="PKZ29_02155"
     CDS             complement(1984..2535)
                     /locus_tag="PKZ29_02155"
                     /inference="COORDINATES: protein motif:HMM:NF013492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOG37703.1"
                     /translation="MPKILVYPNKILRKEAKLVVSLDAATKKEIKKLIVELEKGENAA
                     GLAAPQLGISKRFFGIKKSDKKVELFVNPEMVMTWGERVYPIMKNNEGGEEDFLEGCL
                     SVPDYFGTVKRFLKIKVKWQEIKGNKLVPKSKELEGYEAVVWQHESDHLWGKLFIDYI
                     KKEGGKFYRWNGKEMVVADINKI"
     gene            2600..3466
                     /gene="fmt"
                     /locus_tag="PKZ29_02160"
     CDS             2600..3466
                     /gene="fmt"
                     /locus_tag="PKZ29_02160"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOG37704.1"
                     /translation="MVKIIFFGSSEYSVIILKQLLQIPDFKVQAVFTKPDKAVGRQQI
                     ITANPVSAFAKTNHLPLFQPEEFDSNLQSTISNLHPDLGLVVAYGPPYFTQDMIDIPP
                     YKIVNIHPSPLPQYRGATPGPWQIINGETTSAVTFFQIDAFPDHGPIITQIPFDISPD
                     ATATDFYHQAFTLAAQNLETTIKSYLKHPKSLTPQDHAQKSYFPKFTKDTAKIDWSWD
                     PVKLERFVRALNPWPVAWTEVINHQNKIIKMKLFSATVSQNQLIPQLVQIEGKKPVSW
                     SEISSHYIIKTA"
     gene            complement(3982..>4180)
                     /locus_tag="PKZ29_02165"
     CDS             complement(3982..>4180)
                     /locus_tag="PKZ29_02165"
                     /inference="COORDINATES: protein motif:HMM:NF013026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bL28 family ribosomal protein"
                     /protein_id="HOG37705.1"
                     /translation="SLCGKGTKVGRLRSHAQNRTPRTYKANIQKVTLVVGEDKISGKF
                     CTKCLKRIKKETETQKTAPKK"
BASE COUNT         1129 a         1174 c          485 g         1392 t
ORIGIN      
        1 gttacaatat aaactatcca tgaaacgcct tgatcaaatc atccgttttc tttatttaag
       61 tctttttttc cttactcccc tgatttttaa cttccgcaat tctgaactct ttgaactccc
      121 taaaatgcac tttgtttatt ttttgaccat ttttatcgtt tctctccact ttattaactg
      181 gctttcgggt caaactaaac ttgttgtcaa acaccccttt tcttttcctc tccttttgtt
      241 tctcatctcc caaattatca ccacttttac ctctattgac ccctttacct ccttttttgg
      301 ttattactcc cgcctcaacg gcggccttgt ctctattatt acctttacca gcctttttta
      361 ttgccttatt ccctatctca ccccccgttt ccaaaaaaac attatcaaca tctccctttt
      421 ttccggtttt cttgttgccg tttatggcat tttgcagcac tttggcatcg accggcacct
      481 ttgggtccaa gacgtccaaa accgggtttt ttccagcctt ggtcaaccca actggttggc
      541 agcttatctt tgtcttattc ttcccttatc tctctatcag tctcatcaaa cccaaaaccc
      601 caaaatttct ttgctccacc atctcaattc tctggcgctg tttgcctgtc ttctttttac
      661 taaatccaaa tccggtctga ttgccgccgc cgtttctctt ggtatttacc ttctctacca
      721 acttatttcc gacttcaaac accatacttt cccaaaaaat cttaagtcat accttatctt
      781 tattctgttt tttattatta ctttaaccac caaaaatcct ctcacccaaa agttttttac
      841 ctctccccca tcgcctcaac ccaccaattc ccccgccaac ctgattatca ccccatcaca
      901 agatattcgc cgtatcgtct ggcgcggcag tcttgacctt tggcaaaagt ttcctttgtt
      961 cggcaccggg gttgaaactt ttgcctattc ttattattgg actcgtccca tcgaacacaa
     1021 tctcacttca gaatgggact tcctttacaa caaagctcac aacgaatata tcaactatct
     1081 tgccaccacc ggcttaatcg gctttattcc ttatatcttt ttaatcatca cctttctggt
     1141 tgtcttattc aaaaaccgtc actccaaact ttttttaccc tttctcacat cctacctttc
     1201 tattttaatc accaatgccg ccggtttttc tgtggtagtt atttcccttt acttttttct
     1261 ttttcccgct ttatttttgc tccccgccac caaatctccc accccacccc caaaacataa
     1321 atatttaatg gttatcccac tgattgttgg tctgtgtctc acttacctta atctgacttc
     1381 ctatctggcc gatatttatt acgccaagtc cgaatctgct gataatcggc aaaattaccc
     1441 cgatgcctac aaatatatca atctcagtct caagttaaac ccctaccaac ccacctatct
     1501 tatctatcat gccctggctt ccgccaagat ggctgtagct actcaagatc aatcttatct
     1561 ccaacccgcc atcacttccg ccaaccgctc tgttgccatt tctcccgcca atcttaattt
     1621 ttggcgccaa cgggctcaag tctactatta cttatccggt tttggctccg actattttat
     1681 taaaaccgtc gaatctatga ttcaggcttc caaactcgct cccactgacc ccaaaaacta
     1741 ctattctcta gctcaatttt tagaatctgc ccagcttacc gacgatgcta ttttttatta
     1801 ccagcaagcc atcaaactta aacccaatta cgaccatgct tacttttctt taggcaaaat
     1861 ttactttgac aaaaaggact accaccccgc cgctgacaat ctccaacaag ccgtcaatta
     1921 ttcccacccg gtcaacaccg aagcccaaaa actccttgac caaattaaaa catcttctaa
     1981 accctaaatt ttatttatat ccgccactac catttccttc ccattccacc gataaaattt
     2041 accgccctct tttttgatat agtctataaa caatttcccc cacaaatgat ccgactcatg
     2101 ctgccacacc actgcctcat acccctccaa ctcttttgat tttggcacca atttgttccc
     2161 tttaatttcc tgccatttta cctttatttt caaaaatctt ttgactgtcc caaaataatc
     2221 cggcactgac agacacccct ccaaaaaatc ctcctctccc ccctcattat ttttcattat
     2281 cggataaacc ctctctcccc aagtcattac catttccgga ttaacaaata attccacttt
     2341 tttgtcgctc tttttaattc caaaaaatct tttggaaatt cccagctgcg gcgctgccag
     2401 tccggctgca ttttctcctt tttccaattc gactattaat tttttaattt ctttttttgt
     2461 cgccgcatcc aaactcacca ccagcttggc ttcttttctc aaaattttgt tgggataaac
     2521 taatatcttt ggcatagtct tattttaaca aatcatctcc cgctgactaa atttccccct
     2581 atcagtgata aactcagcta tggttaaaat tattttcttt ggttcttctg aatactcggt
     2641 tatcattcta aaacagcttc tccaaatccc ggattttaag gttcaagccg tctttactaa
     2701 gcccgacaaa gctgttggcc gccaacagat catcaccgcc aatcctgtct ccgcttttgc
     2761 caaaaccaat catctccccc ttttccaacc cgaagaattt gattccaatc tgcagtcaac
     2821 catctccaat ctccaccctg accttggttt ggtggttgcc tacggcccgc cttattttac
     2881 ccaagacatg attgatattc ccccctacaa aatcgttaat attcatccct cccccctgcc
     2941 ccaataccgc ggtgccaccc ccggcccctg gcagattatc aatggcgaaa ccacttcggc
     3001 tgtcactttt ttccaaattg acgcctttcc cgaccacggc cctattatta cccagattcc
     3061 ttttgatatt agccctgatg ctactgccac cgatttttac catcaagcct tcactcttgc
     3121 cgcccaaaat ctagaaacta ccatcaaatc ttatcttaaa catcctaaat cattaacccc
     3181 tcaagatcac gctcaaaaat catattttcc caaatttacc aaagatacag ccaaaattga
     3241 ctggtcatgg gatccggtca agctcgagcg ttttgtccgg gctctcaacc cctggccggt
     3301 tgcctggacg gaagtcatca accatcaaaa caaaatcatc aaaatgaaac tcttttctgc
     3361 taccgtttct caaaatcaac ttattcccca actggttcaa attgaaggca aaaaacccgt
     3421 ttcctggtca gaaatttcct cccactatat catcaaaact gcctgaatta acgcatttta
     3481 tcaacatatt taagcttttt agtccatttt tttaaaaaac tatttttaaa ttattcccaa
     3541 ccaaaggcat aatttaaact aaccttttat ttttaatgga aaaattcatc attattgata
     3601 caaacttttt ctgataactt ccaataattt taggactgta tatttttatt tttcaaaaaa
     3661 atatttttat aaaacttaaa tatacccaaa aataaggtat aaaaacaaaa atatttttct
     3721 aaaaaacttc tcaaccaaat ttattaaaac aaaaattatc cccaaacatc ggcatttcac
     3781 tttttgacaa aaacttccaa aaacttttta attatttcaa ggaggtaaaa ataatgccag
     3841 caaaaaagaa agttgctaaa aaaccagtca agaaagctgt caaaaagaaa gtcgccaaaa
     3901 aaaaggtcgc taaaaaagcc aaaaagactg ccaaaaaagc aaaaagaaaa taaatcagga
     3961 aatttttact aaaaacggtg tctatttttt aggcgccgtt ttttgtgtct cagtctcttt
     4021 tttaattctt ttcaaacact tagtgcaaaa tttaccgctt attttgtcct cgcccaccac
     4081 caaagtcacc ttttgaatat tggctttata tgttcttgga gtcctatttt gggcatgcga
     4141 ccgcagtcga ccgactttag tccccttgcc gcataaacta
//
LOCUS       DAOSHV010000047         4145 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_19473_length_4145_cov_7.714670, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000047 DAOSHV010000000
VERSION     DAOSHV010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 4145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4145
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19473_length_4145_cov_7.714670"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            253..909
                     /locus_tag="PKZ29_02170"
     CDS             253..909
                     /locus_tag="PKZ29_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37706.1"
                     /translation="MNLKEGYLEDRARMVEAATLEMMHNSEVKLGLSDLDNFEGGMLL
                     SDQRKVDILGCVIGVKPAAMMKGWLEAGDFGEEVELGQIISRLGLEWAMEIKPVERKF
                     EVSLFVARDKETLEQLREISQQSTKDVRKLARLQGYPETGVEVMLGEEEALEGEVIVD
                     LARKDMAYMSFFAPPMMSLKNHRREVANYSRLLISSTKKNLPRTYEKAVNRWNTIRND
                     "
     gene            935..2035
                     /locus_tag="PKZ29_02175"
     CDS             935..2035
                     /locus_tag="PKZ29_02175"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOG37707.1"
                     /translation="MFDFRNWLIEEGKLIQEFLKNEGVFVVEFFKKFLGWAEWIKVQT
                     SKLMYRQRGRFSQSFVNVAMFTLFFLVILFSGTLENLVINSEGSETSGSNYLLAAANQ
                     AGANTLISNLPKGETTEYRVQEGDTVSSISQKFGVNIDTIIWENNLKSVGAIKVGQIL
                     KILPVSGVKHTVKRGETIYSIAKDFQVDAQNIIDYPFNTFANDETFSLSAGQELIIPD
                     GIKPKEVVIDKTRYTARTVAPIPGVVGEGNFMWPTSGAISQRYAWYHQAVDIANRSNP
                     DVVASQGGTVVTAGWNAGGYGNYVVIDHGNGYKTLYAHLLNNSIVVKAGQRVSQGQKL
                     GVMGSTGRSTGTHLHFEVISSKGKLNPLNVLK"
     gene            2332..>4145
                     /locus_tag="PKZ29_02180"
     CDS             2332..>4145
                     /locus_tag="PKZ29_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37708.1"
                     /translation="MNKFLRIYTRLVLALMPLFFLPVIYDGFGLGKVSFLVLSGGLGL
                     IFWVVEAIWGKKTEVKFSKGWWLIVLLGVWSVVSFLRMSQGAQARSITSPLGIGSLVG
                     LIFWFFLWLQVRSREEYEKQLNWLTVSAVLVGITSLVVFVMPESKLPFNWPKDNPVIS
                     ISNSWSVTGSLLSEVVLFGFLTVEWVRRLVKKIKNRAEFADYFKEAIVAAFLGLMVVV
                     AGYRLVKLGWLYLDLTTSWVIAVETLKNSPLFGVGLGNFVEAFSRFRPASFNMTDMWV
                     NMFGVSLMGIFHWWTELGLVGLGLILILMVSSWKQKNKLGLVLLGSVVLLTPPVFLTV
                     FLMFWVMATFLGETKEAQMILEAGETKKNIMPYIVGLLVLVIVGFAGYKMVKPVVADY
                     YWRESLTATVKNDATGAYNNQIKAIGMNPNLADYRAMYSQTNLALARNYFEVPKGEEM
                     TEENKQKASTLIQQAVREAQAAVGLDQKMSGYWVNLGSVYRSLIGLIDNTFDWSVQSY
                     QQAAVFDPVNPNIKMELGSLAYGAKDYASAERYFEDAVNAKPNLANAWYNWAYAAKQQ
                     NKIQEAVTRLDQSVKLVPADSEDYEKANKELTEWKKEL"
BASE COUNT         1229 a          450 c         1205 g         1261 t
ORIGIN      
        1 tgcgatgcag gctcaaacac gttccaaaat tatgatttcg ggttgaagta aattattaag
       61 attggatttt aaggcaaaat tatgtcattt cattccaaat tttgacccga aaatctcaat
      121 aagtttgtca agttcataaa atagaaagat ttataaaaca agtttccttt gttaaaggga
      181 gatttagagg gattgtgggg ttgaaaattg gtcttagatt tggtataatt taggtataga
      241 agttaaataa aaatgaatct aaaagaaggt tatttagagg acagagctag aatggtggaa
      301 gcggcaactt tggagatgat gcacaattcg gaagtaaagt taggcttgag tgatttggat
      361 aattttgaag ggggaatgtt gttgtcagat cagcggaagg tggatatttt aggttgtgta
      421 attggggtta agccagcggc gatgatgaag ggttggttgg aagctgggga ttttggggag
      481 gaagtggagt tgggtcagat aattagtcga ttggggttgg agtgggcgat ggaaattaaa
      541 ccggtagaaa ggaaatttga agtaagtttg tttgtggcta gagataaaga aactttggag
      601 caattgagag aaataagtca gcaatcaact aaagatgtta gaaaattagc tagacttcag
      661 ggttatccgg aaacgggagt agaggtgatg ttgggagaag aagaggcttt ggaaggggaa
      721 gtaatagttg atttagcaag aaaggatatg gcttatatgt cgttttttgc gccgccaatg
      781 atgtctttga agaatcatcg tcgggaagtg gccaattaca gtcgattatt gataagttca
      841 actaaaaaga atttgccaag gacttacgaa aaagcagtga atcggtggaa tactattcgt
      901 aatgattgaa tgggtttagt ataataaaag caccatgttt gatttcagaa attggttgat
      961 tgaggagggt aaattaattc aggaattttt gaaaaatgaa ggggtgtttg tggtggaatt
     1021 ttttaaaaaa tttttgggct gggcggaatg gattaaagtg caaacatcga aattgatgta
     1081 tcggcaaagg gggaggtttt cacaaagttt tgtaaatgtg gccatgttta ctttgttttt
     1141 tttggtgatt ttgttttcgg gaacgttgga gaatttggtg ataaacagcg agggaagtga
     1201 aaccagtggg agcaattatt tgttggcggc ggcgaatcag gcgggagcca atactttaat
     1261 ttcgaattta cctaaagggg agacgacgga atatcgagtg caggagggag atacagtctc
     1321 aagtatttct caaaaattcg gggtaaatat agatacgatt atttgggaaa ataatttgaa
     1381 gtcggtagga gcaatcaagg tgggacagat attaaaaatt ttgccagtca gcggagtcaa
     1441 gcatacggtt aaaagagggg agactattta tagtattgcc aaggattttc aggtggatgc
     1501 tcagaatatt attgattatc cgtttaatac ttttgccaat gatgaaactt tttcgttgag
     1561 tgcgggtcag gagttgatta ttcctgatgg aattaagccg aaagaggtgg tgattgataa
     1621 aactagatat acggcgagga cggtggcgcc aataccgggg gtggtgggcg agggtaattt
     1681 tatgtggccg acatcgggag cgatttctca gaggtatgct tggtatcatc aggcggtgga
     1741 tattgccaat cgatcaaatc cggatgtggt agcatcacaa gggggaacag tggtgacggc
     1801 gggttggaat gctggtggtt atgggaatta tgtagtgatt gatcatggta atggttataa
     1861 aactttgtat gctcatttgt tgaataattc aatagtggtc aaggctggtc agagggtgag
     1921 tcaagggcaa aagttgggag tgatgggaag tacaggtcgg tcgacgggaa ctcatttaca
     1981 ttttgaggtg attagcagta aagggaagtt gaatccgctg aatgtactta aatagttcat
     2041 aaagtttatc aagttcatca agtggaaagt ttgtcaagtt tgtcaagttc atcaagttta
     2101 ttcccgattc agctaaaact tcatcgggat tggattttaa ggcaaaatta tgtcatttta
     2161 ttccaaattt tgacctgaaa atttcaacaa gtttataaag tagaaagatt tataaaacaa
     2221 gtcttccttg ttaaagggag atttagaggg attgtggggt tgcaaggatt gtgcggagaa
     2281 aagagggttt aaaaggttgt cgagaggaga tgattttgat aggatttgat tatgaataaa
     2341 tttttaagaa tttacactag gttagtgttg gcattgatgc cattgttttt tttaccggtt
     2401 atttatgatg gttttggact agggaaagtc agttttttgg tattgagtgg ggggttgggt
     2461 ttgatttttt gggtggtgga ggcaatttgg ggtaaaaaga cagaagtaaa atttagtaaa
     2521 ggttggtggt tgatagtatt gttgggggtt tggtcggtgg tgtcgttttt gagaatgagt
     2581 caaggagcgc aggcaaggtc aataacttca cctttgggga ttggtagttt ggtgggattg
     2641 atattttggt ttttcttgtg gttacaagtg agaagtcggg aagaatatga aaagcaatta
     2701 aattggttga cagtttcggc ggtgttggtg ggaataactt ctttggtggt gtttgtgatg
     2761 ccagaatcaa aattaccgtt taattggcca aaagataatc cagtaatatc gattagtaat
     2821 agttggtcgg tgactgggtc attgttgtcg gaagtggtat tgtttggatt tttgacagta
     2881 gaatgggtga gacgattggt taagaaaatt aaaaataggg cagaatttgc ggattatttt
     2941 aaagaagcaa tagtggcggc gtttttggga ttgatggtgg tagtggcggg atatcgattg
     3001 gtgaaattag gatggttata tttggatcta acgacatctt gggtgattgc ggtagaaact
     3061 ttaaaaaaca gtccgttgtt tggagtggga ttggggaatt ttgtggaggc attttccaga
     3121 tttagaccag caagttttaa catgacagat atgtgggtga atatgtttgg ggtgtctttg
     3181 atggggatat ttcattggtg gacagagttg ggattggtag gattgggatt gatattgata
     3241 ttgatggtgt cgagttggaa gcagaaaaat aaactgggac tggttttgtt ggggtcggtg
     3301 gtattattga cgccgccggt gtttttgaca gtatttttga tgttttgggt gatggcgacg
     3361 tttttggggg agacgaaaga agcacaaatg attttggaag caggtgaaac taaaaagaat
     3421 attatgcctt atatagtggg gttgttggtg ttggtaatag ttggatttgc tggttataag
     3481 atggttaaac cagtggtagc tgattattat tggcgagagt cattgacagc gacggttaag
     3541 aatgatgcta caggggcgta taacaatcaa ataaaagcca ttgggatgaa tccgaattta
     3601 gctgattatc gggcaatgta ttcgcagacc aatttggcgt tggcaaggaa ttattttgag
     3661 gtgccaaagg gtgaagaaat gacggaagaa aataaacaaa aagcatcgac tttgatccag
     3721 caggcggtca gagaagctca ggcggcagta ggtttggacc aaaaaatgtc gggttattgg
     3781 gtaaatttag gttcggttta tcgatcgctg attggcctga ttgataatac ttttgattgg
     3841 tcggttcagt cttaccaaca ggcagcagtg tttgatccgg tcaatccaaa tataaagatg
     3901 gagttgggat cgttggctta tggagccaaa gattatgctt cggcagaaag atattttgag
     3961 gacgcggtca atgctaaacc gaatttggct aatgcttggt ataactgggc ttatgccgcc
     4021 aaacagcaga ataaaattca ggaggcggta acaaggctgg accaatcggt aaagctggtg
     4081 ccggcagatt cagaggacta tgaaaaggca aataaagaac tgacggaatg gaaaaaagag
     4141 ttgga
//
LOCUS       DAOSHV010000048         3973 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_20721_length_3973_cov_7.108729, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000048 DAOSHV010000000
VERSION     DAOSHV010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 3973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3973
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20721_length_3973_cov_7.108729"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            91..1173
                     /locus_tag="PKZ29_02185"
     CDS             91..1173
                     /locus_tag="PKZ29_02185"
                     /inference="COORDINATES: protein motif:HMM:NF019522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HOG37709.1"
                     /translation="MKWIKWLVIVVSLVMMGTGVVAAQENKNFEAKIMKVVEEKEIEV
                     GGQRQKYQKLELKLTKGFNQGKTVIVENGHQPLANVVVYKEGDRVIVSMGEDLEGKEI
                     YTIVDYVRKDGLVLLTIIFVALTLIVARLKGLMSILSMVFTFGVVFVYILPQLSLGKS
                     PVLVAVLASVIIIPISFYMAHGVSQKTTVAVVSSLLSLGITAGLSLLFIRLGRLTGLA
                     SEEAGMLLIQRSGDFDMKGLLLSGIIIGALGVLDDITVAQAGVVNELIKSGEKTNKIK
                     IYKQAMNVGRDHISSMVNTLVLAYAGASMPLLLIFVDNPHPLGEIINYEFLAEEIIRT
                     MVGSIGLILAVPITTAIAVNWIKKDK"
     gene            1170..1853
                     /locus_tag="PKZ29_02190"
     CDS             1170..1853
                     /locus_tag="PKZ29_02190"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HOG37710.1"
                     /translation="MKLKLVILIAELAVLGWLWQRNGQVIKNIVSPQGERIIKTTPLP
                     TLEPTLTPTETEKPVITAAPTKKMVTKTPAETDTRPWGVAQQIDEVTWTLRVGNDEKM
                     ATAGEILTALNHYRQRYGSQILTWDDNLAQLARKRAKDINVAKTTDKHVGFKNFMENE
                     DGFNKLGFNSVGENIAWGYKLNGVHTIEWMYGGDEAHNNNQLDNKWNYVGIGVDGLAS
                     CLIFGTGKF"
     gene            1872..2432
                     /locus_tag="PKZ29_02195"
     CDS             1872..2432
                     /locus_tag="PKZ29_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOG37711.1"
                     /translation="MTKTQENLLKAFAGESQARNRYTQFAKMARKQNFEWIARVFEET
                     ADQERTHAEEEYEMITEIVTVKDDLSIKPYSKETVENLKYAAEGERHEWTEMYPSFEE
                     QAKIDGDKEAERLFREIKEVEEKHEERYLILAKHMDADTLYNQEKEMEWKCLNCGYIH
                     KGKTPPTTCPVCKKPFTWYMALGLIR"
     gene            2465..2536
                     /locus_tag="PKZ29_02200"
     tRNA            2465..2536
                     /locus_tag="PKZ29_02200"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2498..2500,aa:Arg,seq:ccg)
     gene            complement(2554..3123)
                     /locus_tag="PKZ29_02205"
     CDS             complement(2554..3123)
                     /locus_tag="PKZ29_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37712.1"
                     /translation="MKKPIVFALIFASSLFLSACTGPKNSSPADSDKSDTPNPQSQET
                     FSLRDLIAKNIPQKCTYSGQNQEGNFDSEIIIQGHKFKQTINSHTSEGKIQLHTISDG
                     EYFYSWGAGPNPKSGLKIKADFATPTDTNSDFDKLKTNQADLDTAYQGSCSPTVVSES
                     DFSLPPDIVFQDYTEFLEKIQSNFHNPGN"
     gene            3180..3686
                     /locus_tag="PKZ29_02210"
     CDS             3180..3686
                     /locus_tag="PKZ29_02210"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOG37713.1"
                     /translation="MKDEAIKIKDNLYYDERSAGGIVFKEENGKIYWLIIKTISGFKP
                     KKKRNKKPVYKFPKGHLKKGEFLKQAALREVEEEGQIKAKILSKIGSNNYILWDEEKR
                     KKIIKKVTFFLMEYTGPSSLKYFDSEMVVERDWFSFEDANEKLEYDNEKVLLKKAKTK
                     LDGLMRNR"
     gene            3729..3815
                     /locus_tag="PKZ29_02215"
     tRNA            3729..3815
                     /locus_tag="PKZ29_02215"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3763..3765,aa:Ser,seq:gga)
BASE COUNT         1312 a          561 c         1058 g         1042 t
ORIGIN      
        1 gcaacctttg caacctttcg gagctttagg aggacgaagt ggcggagcag accgccagtt
       61 taggtagtga tggcgatgat aaactaagat atgaaatgga taaaatggct ggtaatagta
      121 gtaagtttag tgatgatggg aacgggagtg gtggcagcac aagagaacaa aaattttgag
      181 gcaaaaatta tgaaggtggt ggaggaaaaa gagattgaag ttggaggaca aagacaaaaa
      241 tatcaaaaac tggaactaaa attgactaaa ggttttaacc aaggaaaaac agtgatagta
      301 gagaatggtc accagccgct ggcaaatgtg gtggtttata aggagggaga cagggtaata
      361 gtttcgatgg gcgaagattt ggagggaaaa gaaatttata ctattgttga ctatgtcagg
      421 aaagatgggc tggtgttgtt gacaataatt tttgtggctc tgacgttgat agtggcaagg
      481 ctgaaagggt tgatgtcaat tttgagtatg gtgtttacgt ttggagtagt gtttgtttat
      541 attctgccgc aattatcgtt gggaaaaagt ccggtattgg tagcagtatt ggcttcggtg
      601 ataattattc cgatttcttt ttatatggct catggagtga gtcaaaaaac gacggtggcg
      661 gtggttagct ccttgctgtc gttgggaata acggccggat tatcgctact atttattcga
      721 ctagggaggc tgacgggact ggcttcggag gaagcaggca tgctactgat acaaagatcg
      781 ggtgattttg atatgaaagg gttgttgttg tcagggataa ttattggagc tttgggggtg
      841 ttggatgata ttacagtggc acaagcggga gtggttaatg aattgattaa aagcggagaa
      901 aagactaata aaattaaaat atacaaacag gcgatgaatg tgggtagaga tcatatttcg
      961 tcgatggtga acacgttggt attggcttat gccggagctt ccatgccgct gctgttaatt
     1021 tttgtggata atccccatcc gttgggagaa attattaatt atgaattttt ggcggaggaa
     1081 ataatcagga cgatggtggg cagtattggg ttgattttgg cagtgccgat aacgacggcg
     1141 atagcagtta attggatcaa aaaggataaa tgaaactaaa attggtgata ttaatagcag
     1201 agctggcggt gttggggtgg ttgtggcaaa ggaacggtca agtgataaaa aatattgtca
     1261 gtccacaagg ggagagaata attaagacaa ctcccctgcc aaccctagag ccgacattaa
     1321 caccaactga gacagaaaaa ccggtgataa cagcagcacc aaccaaaaaa atggtaacta
     1381 aaacaccggc cgaaacagac accagaccat ggggagtggc gcagcaaatt gatgaagtaa
     1441 cctggacgct aagagtcgga aatgatgaaa aaatggcaac ggcgggagaa attttgacgg
     1501 ctttgaatca ttatcgacag agatacggat cacagatcct gacttgggat gataatttgg
     1561 ctcaattggc tcgaaaaaga gccaaagaca taaatgtggc aaaaacgacc gataaacatg
     1621 ttggttttaa aaattttatg gaaaatgagg atgggtttaa taagttggga tttaattcgg
     1681 tgggagaaaa tattgcctgg ggatataaat taaacggagt ccatacaatt gagtggatgt
     1741 atggcggcga tgaggctcac aataacaacc agctggataa taaatggaat tatgtcggga
     1801 ttggggtgga cgggttggcc agctgcctga tttttggaac aggaaaattt tagatgtggt
     1861 aggatattga tatgacaaaa acacaagaaa accttctgaa agcttttgcc ggtgaaagcc
     1921 aagcaagaaa ccggtatacc caatttgcca aaatggcaag aaaacaaaat tttgagtgga
     1981 ttgccagagt gtttgaagaa actgccgacc aagaaagaac tcatgcggag gaagaatatg
     2041 aaatgattac ggaaatagtg acggtaaaag atgatttgtc gattaaacct tatagcaaag
     2101 agacagtaga aaacctaaaa tatgccgccg aaggagagag acacgagtgg acggaaatgt
     2161 atccttcgtt tgaagaacag gcaaaaattg atggtgataa agaagccgaa agactgttta
     2221 gagaaataaa agaagtcgaa gaaaagcacg aggaaagata tttgattttg gccaagcata
     2281 tggatgctga tacattgtat aaccaagaaa aagagatgga gtggaaatgt ttaaactgcg
     2341 gttatatcca caagggcaaa actccgccaa ccacctgtcc ggtgtgcaaa aaaccattca
     2401 cttggtatat ggctctagga ctaattcgat aagctgtttt tttgataaaa taggtctgat
     2461 tctagtttcc gtagtttaat ggatagaatt ctcccctccg aagggagtga tgtgagttcg
     2521 attctcaccg gaaacacaga ctagagagac gagttagttg ccggggttgt gaaaattact
     2581 ttgaattttt tcgagaaact cggtgtaatc ctgaaaaaca atgtctgggg ggagagaaaa
     2641 gtcggactca gaaactacag tgggtgagca actgccttga taggcggtgt ctagatcggc
     2701 ttgattggtt ttgagcttat caaaatcgga gttggtgtcg gttggggtgg caaaatcagc
     2761 tttgattttt agacctgact tggggttagg accggcgccc cagctataaa aatactcacc
     2821 atcactgatg gtatgaagct ggattttacc ctccgaagtg tgagaattaa tagtttgttt
     2881 aaatttatga ccttgaataa taatttcgct gtcaaaattt ccttcttggt tttgaccgga
     2941 ataggtgcat ttttggggaa tatttttggc aataaggtca cggagagaaa aggtttcttg
     3001 agattgaggg ttaggggtgt ctgatttgtc agaatcagcg ggagatgagt ttttggggcc
     3061 ggtgcaagcg gacaaaaaca aagaagaggc aaaaataagg gcaaaaacga ttggtttttt
     3121 cataaatggg caaataataa ttaattaatt ctagcatttc gggatataat catttaccaa
     3181 tgaaggatga agccataaaa attaaagaca atctttatta cgacgagaga tcggcggggg
     3241 gaattgtgtt taaggaagaa aatggaaaaa tatattggct gataataaaa acaatttcgg
     3301 gatttaaacc aaaaaagaag cggaacaaaa aaccggtgta taagtttccc aaagggcatc
     3361 tgaaaaaggg agaatttttg aaacaagcgg cgttaagaga agtggaagag gagggtcaga
     3421 ttaaagccaa aattttgtca aaaattgggt caaataacta tattttgtgg gatgaggaaa
     3481 aacgaaaaaa gattatcaaa aaagtgacat tttttttgat ggaatatacc ggtccaagca
     3541 gtttaaaata ttttgacagt gaaatggtgg tggaaagaga ttggtttagc tttgaggatg
     3601 ctaacgaaaa gttggaatat gataatgaaa aagtgttgtt gaagaaggca aaaactaaat
     3661 tagatggttt gatgagaaat agatagttat ttttgttaaa ataggacact tttacacttt
     3721 gtaaagttgg aggagtcgcc tagtggttta tggcgcacgc ctggaaagcg tgtgttcgaa
     3781 aggactcgtg ggttcgaatc ccacctcctc cgccaatttt cactttttgt gaaatcgtaa
     3841 gatgttgcct cggcgcaaag cgcctcggca tggtattttt ccgcaatttt gaaggcattt
     3901 ttgaaatcgc acgccagcgt gcggtcggca atgcggaagt tcgaaccaat ctttttgaga
     3961 aaatcccgaa tcc
//
LOCUS       DAOSHV010000049         3971 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_20737_length_3971_cov_5.064607, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000049 DAOSHV010000000
VERSION     DAOSHV010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 3971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3971
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20737_length_3971_cov_5.064607"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(144..911)
                     /locus_tag="PKZ29_02220"
     CDS             complement(144..911)
                     /locus_tag="PKZ29_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37714.1"
                     /translation="MGEVNQAKSYKYLFFITLILLLGVLVGLYFFLNYKDSQSNTVSP
                     DRANSPTQIPTDNKINPTLVPTTTSTKSTLTEKDANDNLYTNNRFGFSLTIPKFVTSS
                     TCGEQKFPTMYFESKDIIYLAPESFYLPNPCQKNTTTLEQIENKNNYSLSKLKIYAAA
                     VKNDTELEQFIKSKYGSGCSLGDKTQSPTADIYNVKILGDGKDLEESQCPINFMVDTK
                     YNPTKGVVVIFELGQACNLSQNQDCADSKIVSSLKFL"
     gene            complement(948..1697)
                     /locus_tag="PKZ29_02225"
     CDS             complement(948..1697)
                     /locus_tag="PKZ29_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37715.1"
                     /translation="MKNYKVDDIKNLLPTAKTALVVVPQLSIDSLSAGLALALALKNN
                     QIDTKLFCPQKPDANYSKLSGLDLVTDTHNQNDLVITLDYPLDQIETVNYNQDNGKLN
                     LVVQTKNGSPKVSQNQILIANQSGSADINFILGDESTLSINANMVNSGNWVQISPQNN
                     IKPWAKASLVDQDAPYSEIFTFLLPMLNFNLTHDIAKNLLIGLRVATQSFSINVSPET
                     FEAGAICLRATQTSPDKDNQSQISPNLIPAA"
     gene            complement(1780..3192)
                     /locus_tag="PKZ29_02230"
     CDS             complement(1780..3192)
                     /locus_tag="PKZ29_02230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOG37716.1"
                     /translation="MTSAQPNTFRPPIVTVMGHIDHGKTSLLDKIRSTDVTRREAGGI
                     TQHIGAYQVQGITFIDTPGHAAFCDMRTRGASITDIAVLVVAATEGIMPQTKECIEHI
                     KHSDLPFIVAMNKMDLEGASPDKVKGQLVEIGFTPEEYGGQIAVVPVSAKTGQGIDQL
                     LDLIKLNAEILELKSYPDKPLKAFIIESKLDKSRGPVASLIVQEGTLNLGDQVYADDV
                     LCKVKAIIDDKGQSLKSAGPSTPVEVLGFELVPCIGSLVTPQKHSPSPTSQCKVKYEY
                     DEDNPKLKIILKADVEGTLEALKHSFSDDVYILSEGVGAITDNDVFMAETGHAQIFAF
                     SVNTPKQILKLAEKNQVKVFQSKIIYEIIEDIQSQVLKMLEPTIDENILGEGVVKAEF
                     KIDKVRIAGIQCTKGEITKGDQIHLKRDGKIIKDTKVDGIRHGKDIIDKVKSGSDCGM
                     TFKPYIDFRLNDVIISYNKT"
     gene            complement(3219..>3971)
                     /gene="nusA"
                     /locus_tag="PKZ29_02235"
     CDS             complement(3219..>3971)
                     /gene="nusA"
                     /locus_tag="PKZ29_02235"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOG37717.1"
                     /translation="IEVTPPGFGRIAAQTAKQAIDQKIHEVEKETVIGEFQKKIGSLV
                     PGIVLRSDPYKMVVGVGKTEGICPKDEQIRGEVIQLGSRKWFLLKDIVKDEETGKSEI
                     VLSRRDPEFIKQLFIREVPEVGNKSVTVEKIARSAGERTKVAVSSSQAGVDPVGSCIG
                     QKGSRIQAILNELPSTEKVDVVPFSKNINHFVVQALSPAQDIKVVSIDQNVATVSVPE
                     SQLALAIGGGGENVRLAGQLVGLEIKITQNQE"
BASE COUNT         1120 a          728 c          916 g         1207 t
ORIGIN      
        1 ggcttgagcg tgtttagtaa taatttcctg cttttcttct ttggttaatg ccataagatt
       61 atttggctaa atccggtaaa atctataata ttttggaatg gctcccaaaa aaccgatttt
      121 agtctaggga tgattataac acatcaaaga aacttgagac tactgacaat ttttgaatcg
      181 gcacagtcct ggttttgaga tagattacaa gcctgaccca attcaaaaat aactacaacg
      241 cccttggtgg ggttgtattt ggtatcgacc ataaaattta taggacactg actttcctca
      301 agatctttgc cgtcgcccaa aatttttaca ttgtaaatgt ctgctgttgg agattgggtt
      361 ttgtcgccca aactacaacc tgagccgtat tttgatttaa taaactgctc caattcggta
      421 tcatttttga cggcagcagc gtagattttt agtttagaga gagaataatt gtttttgttt
      481 tcaatttgtt caagagtggt ggtgtttttt tgacaagggt tgggtagata aaaagattcg
      541 ggagctaaat agatgatgtc tttactttca aaatacatag tcggaaactt ttgttctcca
      601 caggttgagc tggtgacaaa ttttgggata gttagggaga aaccaaatct attgttggta
      661 tataaattat cgttggcatc cttttcggtt agagtagatt tggtggaagt tgtggttggg
      721 acaagagttg ggtttatttt attatcagtt ggaatctggg tgggagagtt ggcacggtca
      781 ggagagacgg tgtttgattg gctatctttg taatttagaa aaaaataaag accgacaaga
      841 acacctagca agagaataag tgtaataaaa aaaaggtatt tataagactt ggcttgattc
      901 acttccccca cgagagaatg ttatcacttc atcaaaacga aagcaaatta ggcggcgggg
      961 ataagattgg gactgatttg ggattggttg tctttatcag gagaagtttg ggtggctcta
     1021 agacaaatgg cgccagcctc aaaggtttcc ggagaaacgt tgatagaaaa ggactgagtg
     1081 gcaacacgca agccgataag taaatttttg gcaatatcgt gagtcagatt gaaattgagc
     1141 atcggcaaca agaaggtaaa aatttcggag taaggagcat cctgatcaac caaactggct
     1201 ttagcccaag gtttaatgtt gttttgaggg gatatttgaa cccaattgcc ggaattaacc
     1261 atgttggcat tgatacttaa agtgctttcg tcgcctaaaa taaaattgat atcagcacta
     1321 ccggattgat tggcaatcaa aatttggttt tgagaaactt tgggagaacc gtttttggtt
     1381 tggacgacca aatttaattt gccgttgtct tggttataat taacagtttc gatttggtct
     1441 aaaggatagt ctaaggtaat aaccaaatcg ttttggttgt gagtatcggt aaccaaatca
     1501 agaccgctaa gtttggaata attagcatcg ggtttttggg ggcaaaaaag tttggtatca
     1561 atctgattgt ttttgagagc cagagctaaa gctaaaccag ccgacaaact gtcgatactt
     1621 aattgaggaa caacaacgag agccgtcttg gcggttggaa gcaaattttt gatgtcatca
     1681 actttatagt ttttcataag aatacctgct tgtttttaat tcttattaat tattttttat
     1741 agtcttttca aaactaacag caacctgatt tggtgccatt tacgtcttgt tataggatat
     1801 tataacatcg tttagcctaa agtcaatata aggcttaaaa gtcatgccac aatctgagcc
     1861 ggatttaact ttgtcaataa tatctttgcc atggcggata ccatcaactt tggtatcttt
     1921 gataattttg ccgtctcttt tgagatgaat ttgatcgcct ttggtaatct cacctttggt
     1981 gcactgaata ccggcaattc taactttatc aattttaaat tcagctttga caactccctc
     2041 ccctaaaata ttctcatcaa tggtcggttc caacattttg agcacctggg actgaatatc
     2101 ctcaataatt tcgtagataa ttttcgactg gaagactttg acctgatttt tttcggctaa
     2161 cttgagaatt tgcttgggag tgttgacgct gaaggcaaaa atttgggcat gaccggtttc
     2221 agccataaaa acatcgttgt cagtgatggc gccgacacct tcggataaaa tgtagacatc
     2281 gtcagaaaaa ctgtgcttga gagcctcaag agtgccttca acatcggctt tgagaataat
     2341 tttgagttta ggattgtctt cgtcgtattc gtatttgact ttgcattgag aagtgggaga
     2401 tggagagtgt ttttggggag taaccaaact gccgatacaa ggaaccagtt caaaacccaa
     2461 aacttctacc ggagtgctgg gtccggcgga tttaagactt tgacctttgt cgtcgataat
     2521 agctttgact ttgcacaaaa catcgtcggc ataaacctga tcacccaaat tgagagtgcc
     2581 ttcttgaact ataagtgaag ccaccggacc gcgggatttg tctaatttag actcgataat
     2641 aaaggctttg agaggtttgt cggggtatga ttttaactcc aaaatttcgg cgttgagttt
     2701 gataaggtca agcagctgat caatcccctg cccggttttg gcagagacag gaacaacagc
     2761 gatttgaccg ccatattcct cgggagtaaa gccaatttcc accaactgac ctttaacctt
     2821 gtcgggagaa gcgccctcaa gatccatttt gttcatagca acaataaaag gtaggtcgct
     2881 gtgcttgata tgttcgatac attccttggt ttggggcata atgccttcgg tggcagcaac
     2941 aactaaaacg gcgatatcgg tgatgctggc gccgcgggtg cgcatatcgc aaaaagcggc
     3001 atgtccggga gtatcaataa aggtaattcc ttgaacctga taggcgccaa tatgctgagt
     3061 aataccaccg gcttcacgac gggtgacgtc ggtagacctg attttgtcca aaagactggt
     3121 tttgccgtgg tcaatatgac ccatgacggt aacgataggc ggacgaaaag tattaggttg
     3181 agctgatgtc atattttgaa aaaccagaaa ctatgaattt attcctgatt ttgggttatt
     3241 ttaatttcca gtccgactaa ctggccggca agacggacat tttctccacc accgccgata
     3301 gcgagagcta gctggctttc ggggacagaa acggtggcaa cattttgatc aatagaaaca
     3361 actttgatgt cttgggcagg agaaagggcc tgaaccacaa aatgattgat atttttggaa
     3421 aaaggcacca catcgacttt ttcggttgaa ggaagctcgt tgaggatggc ctgaattcgg
     3481 gaaccttttt ggccgatgca agaaccgaca gggtcaactc cggcctgact ggaagaaacg
     3541 gcgactttgg ttctttcgcc ggcagatctg gcaatttttt cgacagttac cgatttgttg
     3601 ccgacttcag ggacttcgcg gataaataat tgtttgataa attcagggtc gcggcgggaa
     3661 aggacaattt cgcttttgcc agtttcttcg tctttgacga tgtctttgag caaaaaccac
     3721 ttgcggctgc caagttggat aacttcaccg cgaatttgtt cgtctttggg acagatacct
     3781 tcggttttgc cgacaccgac aaccattttg tagggatcgg agcgtaaaac gataccggga
     3841 actaaactgc caattttctt ttggaactca ccaattacgg tttctttttc aacttcgtga
     3901 attttttgat caatagcctg cttggcggtt tgagcggcaa tacgaccaaa accgggcgga
     3961 gtaacttcaa t
//
LOCUS       DAOSHV010000050         3966 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_20788_length_3966_cov_4.830734, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000050 DAOSHV010000000
VERSION     DAOSHV010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 3966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3966
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20788_length_3966_cov_4.830734"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            165..1220
                     /locus_tag="PKZ29_02240"
     CDS             165..1220
                     /locus_tag="PKZ29_02240"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HOG37718.1"
                     /translation="MIFFFISIFVSFIISLFLTPAVRNFFISRHWVEDPVVKNQKTHN
                     ATAVSPVPRGGGIPIFVAITVTSLLLLPLDKHLLGILLAALVSLVVGVWDDIKDISPL
                     SRFLTNILAALIVVGSGIGIAYISNPFTGGVFDLSFLKINFNFLGDHSIWVISDLIAI
                     VWIVWCMNIVGWATGVDGQLPGFTAISAFFIGLIGFRYSTDITQWPVIILSGAVCGAY
                     LGFLPFNFFPQSIMPGYSGKSLAGFFLAVLSILSGAKMATLLFLLAIPMIDAVYAIVR
                     RLIHHRPIYLGDGQHFHHQLLKQGFSRRSIALIYWSFSLILGLISLFLNSSQKAYVFI
                     WFAFLFFSFLFHFSRRN"
     gene            complement(1186..2010)
                     /locus_tag="PKZ29_02245"
     CDS             complement(1186..2010)
                     /locus_tag="PKZ29_02245"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOG37719.1"
                     /translation="MKHKGAQLALVIISSVFLGGCLVSTKKSAIEINSYPVAKVYIDG
                     KEMGLTPYRNRQLKPGEIEVRLIANESEWSKKIKLQNNISTVVDWEFGKTEDESGGYV
                     LYLEKTGDKKSAGLMINTNPNKSTVTIDNEVKGLSPMRMSNIGVGDKHLALSFPGHKT
                     MDVFVKSLNEYQLVIDAVLKKDKVEVKPEEEVMVDNADLLAKPTVVVVKIKPTETGWL
                     RVREMDSMSSREVGRVAPGETYPLLEEKQEWYKIDLGNNKSGWIAASYGEKSETEN"
     gene            2053..3024
                     /locus_tag="PKZ29_02250"
     CDS             2053..3024
                     /locus_tag="PKZ29_02250"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HOG37720.1"
                     /translation="MKPKIYFFLFLILAFVSGFIYLYFFPKNSPLITPSLNFPSSPLK
                     PSPTPTSTPTVTFGLVGDLGLGRFITATARSKNDFSWSFSGVSHLLQSTDFNLANLES
                     PIITNCPEGKTGTFTFCGDTRFLPYLKQNKFIFNLANNHIYNYGQDGFSQTQTLLSQS
                     QIGFFHSQVPATEFYQTTINSISFGFLGFDLITYPHFNHQKIIDLVSQYQSQVDWLIV
                     SLHWGNEYLPQAETWRHTLAHQLVDAGADIIHGHHPHVLQETEVYHSKPIFYSLGNFI
                     FDQNWSQETSLSQLTLLSVTKDQIISTQDIPLIIKSNSRPELIKQIK"
     gene            complement(3004..3630)
                     /locus_tag="PKZ29_02255"
     CDS             complement(3004..3630)
                     /locus_tag="PKZ29_02255"
                     /inference="COORDINATES: protein motif:HMM:NF037785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HOG37721.1"
                     /translation="MLVKTKRTIKRVAVFGDSEAKKTDRHFIDAYNVAKLLGQKGYIV
                     LNGGGPGVMLAATLGAKVGGGKVETVVIEAKKRLSNYEGSSEDNCNMADKIVYTKNYL
                     ERMGKLVELADAYIILKGGVGTLSEIGLAWQMAKFDYGRHKPLVFLGKEINQAMSDVV
                     ERLEFDKMEIDLYEQVSTPEEAVEVIKNKKGVVKENSGWMGKLFDLFD"
     gene            complement(3614..>3966)
                     /locus_tag="PKZ29_02260"
     CDS             complement(3614..>3966)
                     /locus_tag="PKZ29_02260"
                     /inference="COORDINATES: protein motif:HMM:NF013030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37722.1"
                     /translation="FVKTVKDTGAEAGLAWDIETEIGQVPVETDVVLLLGRKAGFGDE
                     KFNSKVWQKVEQLKEIKQNRNYNFRIALDGGINETNITKAKTAGVEIAYCGRSVFDGK
                     VKDNLEKLIYAGKD"
BASE COUNT          873 a         1034 c          565 g         1494 t
ORIGIN      
        1 cttcctactt tttctttggc tattttgcta attaacaatc ttttttctat cagcctttct
       61 tccaccaaca gtcttcttgc ccgtattctt atttttacct ctcttgccgt ttcctttttc
      121 ttttttatca cccttgtcaa aatagtttat ttggtttcct aaacatgatt tttttcttta
      181 tttctatttt tgtctccttt attatttctc tttttttaac cccggcagtc agaaattttt
      241 ttatttcccg tcattgggtt gaagatccgg tcgttaaaaa tcaaaaaacc cacaatgcta
      301 ctgctgtttc tcctgttcct cggggtgggg gaattcccat ttttgttgcc attaccgtta
      361 cttctttgct actcttgcct ttggataaac atcttttggg tattttgttg gcagctctgg
      421 ttagtcttgt tgttggtgtt tgggatgata tcaaagacat tagcccttta tctcgatttt
      481 taaccaatat tttggcggct cttattgtcg ttggttccgg tattggcatt gcctatattt
      541 ccaatccttt taccggcggt gtttttgatc tttcttttct taaaattaat tttaattttt
      601 tgggcgacca tagcatttgg gttatttccg atctgattgc tattgtttgg attgtttggt
      661 gcatgaacat tgtcggctgg gctaccgggg ttgatggtca gctccccgga tttactgcta
      721 tctcggcttt ttttattggt cttatcggtt ttcgttattc caccgacatt actcagtggc
      781 cggtgattat tctctctggg gctgtctgtg gcgcttatct tggttttttg cctttcaatt
      841 ttttccctca aagcattatg cccggttaca gtggcaaaag tttggccgga ttttttttgg
      901 cggtgctttc tattctttcc ggtgccaaaa tggctaccct tctctttctg ctggctattc
      961 ctatgatcga cgccgtctat gccatagttc gccgcttgat tcaccatcgc cccatctatc
     1021 ttggcgacgg tcagcacttc catcaccagt tgctcaaaca aggttttagt cgccgctcca
     1081 ttgctcttat ttattggtca tttagcttga ttttgggttt aatttccttg tttcttaata
     1141 gctcccaaaa agcctatgtc tttatttggt ttgcttttct cttttttagt tttctgtttc
     1201 acttttctcg ccgtaactag ccgctatcca accgctttta ttgttaccta aatcaatctt
     1261 ataccattct tgtttttcct ccagcagggg ataagtttcc cccggggcca ctctgccgac
     1321 ttctcttgag ctcattgaat ccatctctct gactctcaac cagccggtct ctgttggttt
     1381 aatttttacc actaccactg tcggttttgc cagtaaatca gcattatcta ccattacttc
     1441 ttcctctggc tttacttcca ccttgtcttt ttttagcacc gcgtctatca ctagttgata
     1501 ttcattcaac gattttacaa atacatccat agttttgtgt cccggaaaac ttaacgccaa
     1561 atgtttatct cctactccaa tattgctcat cctcattggc gacaacccct taacctcatt
     1621 atctatagtt acggtgcttt tgttggggtt agtatttatc atcaaccccg cactcttctt
     1681 atcgccggtt ttttccagat acaacacata ccctcctgac tcgtcttcag ttttgccaaa
     1741 ttcccaatca accaccgtgc tgatgttgtt ttgcaattta atttttttag accattccga
     1801 ttcattggct atcaacctca cttcaatctc tcccggtttt aattgccggt ttcggtatgg
     1861 tgttagcccc atttctttcc catcaatata aactttggct accggataac tgtttatttc
     1921 tattgctgat ttttttgtcg acaccaaaca cccgcccaaa aagacgcttg atataattac
     1981 taaagccaac tgtgcccctt tatgtttcat tgtgtgcttt tagtttacca aaacttgcgc
     2041 tccaaattat ccatgaaacc aaaaatatat tttttcctct ttttaattct cgcctttgtc
     2101 tcaggtttta tttatcttta ttttttcccc aaaaactctc ccctgatcac tccgtcgctt
     2161 aattttcctt catcaccgtt aaaaccttca cccactccta cttcaacccc tacggttact
     2221 tttggtttgg ttggtgatct tggtttgggc cggtttatta ctgccaccgc caggtcaaaa
     2281 aatgattttt cttggagttt ttctggggtc tctcacttac ttcaatcaac tgactttaat
     2341 ttagctaacc tcgaaagtcc tattattacc aattgtcccg aaggtaaaac tggcactttc
     2401 actttttgtg gcgacacccg ctttcttcct tatctcaaac aaaataaatt tatttttaat
     2461 ctagccaaca atcatattta caattatggt caagatggct ttagtcaaac ccaaacctta
     2521 ttatctcagt ctcaaattgg ttttttccac tcccaagttc ccgccaccga attttatcag
     2581 accaccatta acagtatttc cttcggcttt ttgggttttg atctcattac ctaccctcac
     2641 tttaaccacc aaaaaatcat tgatttggtt tcccagtacc agtctcaagt tgattggtta
     2701 attgtttccc tccattgggg taatgagtat ttgcctcaag ctgaaacctg gcggcatact
     2761 ctggctcacc aactagttga tgccggtgcc gatattattc acggtcatca cccccatgtt
     2821 cttcaggaaa ccgaagtcta ccactccaaa cctatttttt attctttagg caactttatt
     2881 tttgaccaaa attggtctca agaaacctca ctatctcaac taacccttct ctctgttacc
     2941 aaagaccaaa ttatttccac tcaagatatt cccttaatca tcaaatccaa ctcccgcccc
     3001 gaactaatca aacaaatcaa ataattttcc catccagcca ctgttttcct tgaccacccc
     3061 ttttttattt tttattactt ccaccgcttc ctccggagtt gatacctgtt catacaaatc
     3121 aatctccatt ttgtcaaact ccaacctttc caccacatca ctcatcgcct gattaatttc
     3181 tttgcccaaa aaaaccaatg gtttgtggcg gccataatca aattttgcca tctgccacgc
     3241 caaccctatt tctgataaag ttcccacccc acccttaaga ataatataag catcagccag
     3301 ttctaccagt ttacccatcc tttccaaata attttttgta tatacaattt tgtccgccat
     3361 attgcagtta tcttcacttg agccctcata attgctcaat ctctttttgg cttcaattac
     3421 caccgtttcc accttgcctc cacccacctt tgctcccaag gttgccgcca acattacccc
     3481 cggtccgccg ccgtttagca caatatagcc tttttgtccc agtagtttgg cgacattata
     3541 agcatctata aaatgccgat cagtcttttt ggcttccgaa tcaccaaata cagccactct
     3601 tttaattgtt cttttagtct ttaccagcat agattaattt ttctaaatta tcctttacct
     3661 taccatcaaa cactgaccgg ccgcaatacg ctatctctac tcctgccgtt ttggctttgg
     3721 taatattagt ctcatttatc ccgccgtcca aagctatcct aaaattataa tttcggtttt
     3781 gttttatctc ttttaactgc tccacttttt gccacacttt actattaaat ttctcatctc
     3841 caaatcccgc ttttcgcccc aacaatagca ccacatcagt ttccaccggc acctgaccga
     3901 tttctgtttc aatatcccaa gccaatcctg cctctgcccc ggtatctttg actgttttta
     3961 caaact
//
LOCUS       DAOSHV010000051         3830 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_21888_length_3830_cov_4.668344, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000051 DAOSHV010000000
VERSION     DAOSHV010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 3830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3830
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21888_length_3830_cov_4.668344"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(207..362)
                     /locus_tag="PKZ29_02265"
     CDS             complement(207..362)
                     /locus_tag="PKZ29_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37723.1"
                     /translation="MSKESSLPEISAPKASGTPQSRRKEARRAAKKANKNHGTKRGDK
                     GKQSKPR"
     gene            complement(405..755)
                     /locus_tag="PKZ29_02270"
     CDS             complement(405..755)
                     /locus_tag="PKZ29_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37724.1"
                     /translation="MLNDHELSAVAVFKGVLKPEAFIENFPSNFLARMFASFSKLQPY
                     QQVFQDLKAIANPTRGDINRLWEIIYRIDNDFAEINTYDGFKEVVKKYESFTDFCLHR
                     EPDFQLMLIEHLAT"
     gene            963..1538
                     /locus_tag="PKZ29_02275"
     CDS             963..1538
                     /locus_tag="PKZ29_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37725.1"
                     /translation="MQAYFIASSRLVAKNKEIYKRMYEAIGAENKMVSDKVWQWVKKG
                     VKDLSEATIEEKRDNYKKTVKAINKSDVVVMEISGHSMSMGYILSQALEQNKPVVVLY
                     KKGMEPIFVKGITNPKLVLAEYNQQNLEKVIDGAIGQAKGLVDMRFNFFVSPKILNYL
                     TWVSEKRRIPKAVFLRHLIEREIKKEREFKG"
     gene            complement(1528..2733)
                     /locus_tag="PKZ29_02280"
     CDS             complement(1528..2733)
                     /locus_tag="PKZ29_02280"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HOG37726.1"
                     /translation="MKPQLSLLILVYLRFFARLQVKKIRWLQKLKGKNLDIIGITGSA
                     GKSSTLTACQSVFSKNFRVKTNHGYNSESGLPLSIIGLKINDYSYLSWLKIIFLTPFK
                     FILNWSAYDILILEMGIDSPQWPKNMDYLLSVVQPNIGIFLNVSPVHMFNFNSLEDIA
                     QEKAKLVNQAKVAIINPADKLVLKFTQNKNLIKLTLTDIKFANYHLPDVYQVGFGAAL
                     SLAQLFHIPRAQAIANLQQNFSLPPSRSSILDGINHTTIIDSSYNSSPIAAAKLLNFL
                     STFPGRKIAVLGDMRELGSSTATQHRQIYQLALKSADLIISVGPETTAYFGAKAHKFT
                     YWWQAADFLKSNIKSGDTILVKGSQNTIYLEELVKTILAHPADAAKICRQSPWWLKTK
                     SKFKRLSTP"
     gene            2764..3687
                     /locus_tag="PKZ29_02285"
     CDS             2764..3687
                     /locus_tag="PKZ29_02285"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HOG37727.1"
                     /translation="MKKVKVLVLMGGGGSEHEVSLSSGAEVVKNFDKEKYEIATMVME
                     KANVDIEGIKKVETDVVFIAIHGGVGEDGTIQKMLENEGIKFVGCGSRASEIGIDKIK
                     FKKLMRENNLPTTRSLEVVKGEMVDLDEVKELGSKWVVKPATQGSSVGVTIVTDLDRL
                     DAALETAFEYDDRVLIEEFIEGMEVSCGVLGNNQPTALPVIEIEPKNEFFDYEAKYTQ
                     GKCDEIVPARLAEEVTAKVQEYTLKVFELIGGRGFARVDFMVKDNLEPKILEVNTIPG
                     LTPTSLLPKEAAAVGISYSELLDKMVELARE"
BASE COUNT         1191 a          528 c         1059 g         1052 t
ORIGIN      
        1 aatttttcca ctggaaaatg aggtctgcga tgcaggctca aatacgttcc aaaatgatga
       61 tttcgggttg attgaggatt ggatttttag gtaaaatttc ggcgttgtga cttgaaattt
      121 ggacctaaaa atctcaaaaa aaaagcccgg ggaaacagaa tctttacaga taatgtttgc
      181 ctccgggcaa aatggtggtg gattacttac cggggtttgg actgtttgcc cttatcgccc
      241 cttttggtgc catggttttt gttggctttc ttggcagcgc ggcgggcttc tttgcgacgg
      301 gactgaggag tgccggatgc tttgggcgca gagatttcgg gtagcgatga ttccttgctc
      361 atgattagtc tcctttttat gttaattttt tggaaaccct atggttaagt ggctaggtgt
      421 tcgatcaaca tcagttggaa atcaggctca cgatggaggc aaaagtcggt aaaactttca
      481 tattttttga ccacttcttt gaaaccatcg taggtgttta tttcagcaaa gtcattgtcg
      541 atacggtaga taatctccca aagacgatta atgtcgcctc tggtgggatt ggcgatagct
      601 ttaagatctt gaaagacctg ttgatagggc tgaagcttgc tgaaggaggc aaacattctt
      661 gccagaaagt tactaggaaa attttcgata aaagcttccg gtttgagtac acccttgaaa
      721 accgcaaccg cgctgagctc gtgatcgttt aacataaaat ccacctttct tgttagtttt
      781 aaagtgcaaa tactttaact aagttgaaaa ttgtctcatt tttgagataa aaagtcaaat
      841 tataggatat ctctaaaaaa gatttatgaa ataagattca agatttagga ataaaaaaat
      901 gaattaaaat ttatataaaa tatagcctta tttattgaag tggatttata gtaaaatagg
      961 ctgtgcaagc atatttcatc gcctcgtcaa ggttggtggc aaaaaacaaa gaaatttaca
     1021 agcggatgta tgaggcgatt ggggcggaaa ataaaatggt gagtgacaag gtatggcagt
     1081 gggtcaaaaa aggggtcaaa gacttgagtg aagcgacaat agaggagaaa agagataact
     1141 acaaaaaaac ggtcaaggcg atcaacaaat cggatgtagt ggtgatggaa atttcgggcc
     1201 acagtatgtc gatggggtat attttgagtc aggcgttgga acaaaacaaa ccggtggtgg
     1261 tgttatacaa aaaaggaatg gaaccgatat ttgtaaaggg gattaccaac ccaaaattgg
     1321 tgctggcaga atataatcag cagaacttgg aaaaggtaat tgacggggcg atagggcagg
     1381 ctaaggggtt agtagatatg agatttaatt tttttgtcag ccctaaaatt ttgaactatt
     1441 tgacttgggt gtcggaaaaa aggcgaattc ccaaggcagt gtttttgcgc cacttgattg
     1501 aacgggaaat aaaaaaggaa agggaattta aggggtagaa agacgcttaa atttggattt
     1561 ggttttgagc caccagggag actgacggca gattttggca gcatcggcgg gatgggcaag
     1621 aatggttttg actaactctt cgaggtaaat ggtattttgg gagcctttga ctaaaatagt
     1681 gtcaccggat ttgatattag atttaagaaa atcagctgcc tgccaccagt aggtgaattt
     1741 gtgagctttg gcgccaaaat aggcggtggt ttcgggaccg acactgataa tgagatcggc
     1801 tgatttgaga gctaactgat aaatttgacg atgttgggta gcagtagatg agcccaattc
     1861 tctcatatca cccaagacgg caattttgcg accgggaaag gtagaaagaa agtttagtaa
     1921 tttggcggcg gcaatagggg aggaattgta ggagctgtcg ataatggtgg tgtgattgat
     1981 accgtctaaa atagaagagc gggaaggggg aagagaaaag ttttgttgaa gattggcgat
     2041 agcttgggcg cggggaatat gaaaaagttg ggccaggctg agggcggcgc caaagccgac
     2101 ctgataaacg tcagggaggt gataattggc aaatttgata tcggtgaggg taagtttaat
     2161 cagatttttg ttttgggtaa atttaagaac taatttgtcg gcaggattga tgatggcaac
     2221 tttggcttgg ttgacaagtt tggctttttc ctgggcaatg tcctcaagag aattgaaatt
     2281 gaacatatga actggggaaa cgttgagaaa gatgccgatg ttgggttgaa caacggacaa
     2341 aagatagtcc atatttttgg gccattgggg ggagtcaatg cccatttcta aaatgaggat
     2401 atcgtaggcg gaccaattaa gaatgaattt gaatggagta aggaaaataa tctttaacca
     2461 agagaggtaa ctgtaatcat taattttgag accaataatg cttaaaggca gaccggattc
     2521 ggaattgtag ccatgattgg ttttgacccg aaaatttttg gaaaaaaccg attggcaggc
     2581 agtcagagtg gaacttttgc cagccgaacc ggtaatgccg ataatatcaa gatttttgcc
     2641 tttgagtttt tgaagccagc gaattttttt gacttgaaga cgggcaaaga aacggagata
     2701 gactaagata agcagggaaa gttgtggttt cataggttta ttttatcagg tataatgaag
     2761 cttatgaaaa aagtaaaagt gttggtgttg atgggtggtg gcggtagtga acatgaagtg
     2821 tcattgtctt cgggggcgga agtagtaaag aattttgata aagagaaata tgagattgcc
     2881 actatggtga tggagaaggc aaatgttgat atagaaggta ttaaaaaagt cgagactgat
     2941 gtagttttta ttgccataca cgggggagtg ggcgaggatg gcacaattca aaaaatgtta
     3001 gaaaatgagg ggattaaatt tgttggttgc ggcagccggg catcggagat agggattgat
     3061 aaaattaaat ttaaaaagtt gatgagagaa aataatttac cgacaaccag aagtttggaa
     3121 gtggtaaaag gggagatggt ggatttggat gaggtcaaag agttgggaag caaatgggtg
     3181 gttaagccgg caactcaagg gtcgagcgta ggggtgacaa ttgtgactga tttagacagg
     3241 ttggatgcgg ctttggaaac agcgtttgaa tatgatgaca gggtcttgat agaggaattt
     3301 attgaaggaa tggaggtatc gtgcggggtg ttgggaaata atcagccgac agcgctgcca
     3361 gtaattgaga ttgagccaaa aaatgagttt tttgattatg aggcgaagta tactcaggga
     3421 aagtgtgacg agattgtgcc ggcaagactg gcagaagagg tgacagctaa agtccaagaa
     3481 tacactttga aggtgtttga gttgattggc ggcagagggt ttgctagagt ggattttatg
     3541 gtaaaagata atttggaacc aaaaattttg gaggtaaata caattccggg gttgacaccg
     3601 acgtcgttgc tgccaaaaga agcggcagca gtagggatta gttattcgga gttgttggat
     3661 aagatggtgg agctggcgag ggaataagtt tatcaagttg aaagttcgta aagtttgtca
     3721 agtccatcaa gtaaaaacat tcagatcaac cctcatcatc aatttttcca ctggaaaatg
     3781 aggtctgcga tgcaggctca aatacgttcc aaaattatga tttcgggttg
//
LOCUS       DAOSHV010000052         3757 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_22552_length_3757_cov_4.938682, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000052 DAOSHV010000000
VERSION     DAOSHV010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 3757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3757
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22552_length_3757_cov_4.938682"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            147..1034
                     /locus_tag="PKZ29_02290"
     CDS             147..1034
                     /locus_tag="PKZ29_02290"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HOG37728.1"
                     /translation="MANIYQQISSNKIRSGFILTLFIAFILVVAYIITVSFNLDNSLI
                     IGAIAFSLISSLSGYFAGDKMVLSLNQAKPADKKNYFDFYTVVENLSQVNQTPVPKIY
                     VIDSPAPNAFATGRDPQHAAVCATTGLLNKLNRTELEGVMAHELSHIKNYDTRLMTLV
                     SVLVGSLSILINSATRYSFFSRNNRDNDRQGSGLLSIIGFGLIIFAPIIAQLIQLAIS
                     RRREFLADASAVKLTRQPKGLINALIKISSDPNQMQTASTATASLYINNPFKNNKLAS
                     LFSTHPPIEDRIKALSQML"
     gene            1082..>3757
                     /gene="dnaE"
                     /locus_tag="PKZ29_02295"
     CDS             1082..>3757
                     /gene="dnaE"
                     /locus_tag="PKZ29_02295"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOG37729.1"
                     /translation="MSFVHLHVHTEYSLLDGLNKIKNLVSAVKDMSMNSLAITDHGNM
                     YGAIEFYKACQKSDIKPIIGCETYLAPNGRLDKTSTNRRNQHLILLAKDYRGYKNLMK
                     LISISHIEGFYYKPRIDWETLKKYSQGLICTSACIEGEIAQLILDDNYDQAKTRAKDF
                     QDLFGQDYYLEIQRHPNLEGQDKANEGIIKISRDLGIPLVATNDAHYLKKDDAFAQDV
                     LMMINTQTTINDSKRLSLINIPDFYIKSPEEMSQQFADYPDALENTQKIADKCQLEIE
                     LGKWYFPKFKLPPKKTAEETLHDLVYQQAKNHYGKLTPEIKERLDYELDVINPKGYAA
                     YFLIMYDFIRWTEKHNTPTNTRGSAGGCLVAYVLGITSVDPLIYQLPFERFLNKDRPS
                     PPDIDLDVADDKRQSMLYHIIDQYGQDAVAQICTFGRMMAKGAVRDTARVLGYEYAVG
                     DRISKIIPLGSQGFPMSINKALDTTPELKQMYDTDVDAKKIIDTAKQIEGCARHISVH
                     ACAIVISPTVITDFSPIQTETGGDKPITQYEMHACEDVGLIKYDILGIRNLSFLGQSV
                     LNVKKSRNLDIDIRKIPLDDQKTFDMLSRGSTMGVFQLAGEGMTKWIKELKPNRVEDL
                     MAMVALYRPGPMAIIPEYIARKNDPSKISYFDPKMEKFLQSSYGLLVYQDDCLYTAIE
                     LAGYNWVEVDKFRKAIGKKIPEEMAAQKEKFISGCIENGYTKKKAEEIFKLIEPFTSY
                     GFNKAHAASYGMLAYRTAYMKANFPAEYMCALLTAESGDAETVTDGVSECRHLGIIVS
                     PPDINKSDSGFEMEKNPDSLEGTSIRFGLSAIKNVGNAAIDNILNERNKNGPFLSVSD
                     FCIRVDSQKVNKRVLESLIKVGALDQFGERNAILAS"
BASE COUNT         1116 a          880 c          680 g         1081 t
ORIGIN      
        1 tacaatactg ccattgttac tattccggga atctggattg cccaaatctt tggcttcaaa
       61 gaggaaaggg gattgaatac tcctgatgac aaaaaccagc ttgaagtttc tgccgaagat
      121 atgaaaaatc ctaaggtaaa tctctaatgg ccaatattta tcaacaaata agcagtaaca
      181 aaattcgctc cggatttatc cttaccttgt ttattgcctt tattttagtt gtcgcttaca
      241 ttattaccgt ttcctttaat ttagataata gcctgattat cggagctata gctttttctc
      301 tgatatcatc cctctccggc tattttgccg gcgataagat ggttttaagt ctaaatcaag
      361 ctaaacctgc tgacaaaaaa aattatttcg atttttatac tgtcgtcgaa aatcttagcc
      421 aggttaacca aacccccgtt cctaaaattt atgtgattga tagtccggct ccaaacgcct
      481 ttgctaccgg tcgagatcct caacacgccg ccgtctgtgc caccaccggt ttattgaaca
      541 aacttaaccg aaccgaatta gaaggcgtta tggctcacga actttcccat atcaaaaatt
      601 atgacacccg actaatgact ctagtcagtg tccttgttgg cagcttatcc atcctaatta
      661 attccgctac cagatattct tttttctccc gaaataatcg ggacaacgac aggcagggga
      721 gtggtttgct tagtattatt ggttttggtc ttataatttt tgcccctatt atcgcccaat
      781 taatccaact tgccatttcc cgacgtcgtg aattccttgc cgatgcttcg gctgttaaac
      841 taactcgcca acctaagggt cttatcaacg cccttatcaa aatttcctcc gaccccaacc
      901 aaatgcaaac cgcttctact gccaccgcct cgctttatat caataacccc ttcaaaaaca
      961 acaaacttgc cagtctgttt tctacccacc ccccgataga agaccggatc aaagctctaa
     1021 gtcaaatgct ttgacctaaa gctttatcaa ctttaaactt tgtaacttga taaactaatc
     1081 tatgtctttt gttcatcttc atgtccatac tgaatattct cttttggatg ggttaaacaa
     1141 aatcaaaaac ctagtttctg ccgtcaaaga catgagcatg aattctttgg cgatcaccga
     1201 tcatggcaat atgtatggtg ctatcgaatt ttacaaagcc tgccagaaat cagacattaa
     1261 acccattatt ggttgcgaaa cttatcttgc tcccaacggt cgtctcgaca aaacctccac
     1321 caaccgccgc aatcagcacc tcatcctcct cgccaaggat taccgaggct acaaaaatct
     1381 gatgaaatta atcagtatta gtcatattga gggtttttat tacaaacctc gcatagactg
     1441 ggagactcta aaaaaataca gtcaaggttt aatctgcacc agcgcctgta ttgaagggga
     1501 aattgctcaa ctgattttgg atgacaatta tgatcaggcc aaaaccagag ccaaagattt
     1561 tcaagacctg tttggtcagg attattactt agaaattcaa cgccacccca accttgaagg
     1621 ccaagataaa gctaatgaag gtattatcaa aatcagccgc gatctaggca ttcctttggt
     1681 tgctaccaac gacgctcatt atctaaaaaa agacgatgct tttgctcagg atgttttaat
     1741 gatgataaac actcagacta ccatcaacga ctccaaacga ctcagtctca taaacatccc
     1801 tgacttttac attaaatctc ccgaagaaat gtcacagcag tttgctgact accctgatgc
     1861 tctcgaaaac acccaaaaaa ttgccgacaa atgccagcta gaaattgaac taggtaagtg
     1921 gtattttccc aaatttaagc tccctcccaa aaaaactgcc gaagagacac tccatgattt
     1981 agtctatcaa caggcgaaaa atcattatgg taaattaact ccggagatca aagaacgcct
     2041 tgactatgaa cttgatgtta ttaaccccaa aggttatgcc gcttatttct taattatgta
     2101 tgattttatt cgttggaccg aaaaacataa tactcccacc aacaccagag gctccgccgg
     2161 cggttgtctg gttgcttatg tcttgggtat tacttcagtt gaccctctaa tttatcaact
     2221 tccttttgaa agatttctta acaaagaccg accctcaccc cccgatattg acctcgatgt
     2281 tgctgatgat aaacgtcaaa gtatgcttta ccacatcatc gatcaatatg gtcaggatgc
     2341 ggtggctcaa atctgtactt ttggccgaat gatggccaaa ggcgctgtta gggatactgc
     2401 cagagtctta ggttacgaat atgctgtcgg cgatagaatt agcaaaatta ttcctctggg
     2461 ttctcagggt tttcccatgt ctatcaacaa agctttggac accactccgg aacttaaaca
     2521 aatgtatgac accgacgttg atgccaaaaa aattatcgac accgccaagc aaattgaggg
     2581 ttgcgcccgc catatcagcg tccatgcctg cgccatcgtt atttctccca cggtgattac
     2641 tgatttttct cccatccaaa ctgaaaccgg cggcgataaa cccatcactc aatatgagat
     2701 gcacgcctgt gaagatgtgg gtttaattaa atacgatatc ttagggatca gaaacttatc
     2761 ttttttgggt caatctgttt tgaatgtcaa aaaaagccgt aaccttgata ttgatatccg
     2821 taaaattcct cttgatgacc aaaaaacttt tgatatgctt tcccgtggca gcactatggg
     2881 tgtttttcaa ttagccggtg agggtatgac caaatggatc aaagagctca aacccaaccg
     2941 ggttgaagat ttaatggcca tggtggctct ttaccgtccc ggacctatgg ccattatccc
     3001 cgaatacatt gcccgaaaaa acgacccatc taaaatttcc tattttgatc ccaaaatgga
     3061 aaagtttttg caaagttctt atggtctttt ggtctaccag gatgattgtt tatatactgc
     3121 catcgaactt gccggataca attgggttga agtcgacaag tttagaaaag ctatcggcaa
     3181 aaaaattccc gaagaaatgg ctgcccaaaa ggaaaaattt atttccggct gtattgaaaa
     3241 cggctatacc aaaaaaaagg ctgaagaaat ttttaaactt atcgaacctt ttacttccta
     3301 cggtttcaac aaagctcatg ccgcctctta cgggatgttg gcctaccgca ccgcctatat
     3361 gaaagctaat ttccccgctg aatacatgtg cgccctgctc accgccgaat ccggagacgc
     3421 cgaaaccgtt actgacggtg tcagtgaatg ccgacattta ggcattattg tcagtcctcc
     3481 ggacatcaac aaatctgaca gcggctttga aatggaaaaa aatccggatt ctcttgaagg
     3541 cacctctatt cgcttcggtc ttagtgctat caaaaatgtc ggcaacgccg ccatcgataa
     3601 tattcttaat gaaagaaaca aaaatggtcc ttttttgagt gtcagtgatt tttgtatcag
     3661 agttgacagt caaaaagtta acaaaagagt tttggaaagt ctcatcaagg ttggcgctct
     3721 ggaccaattt ggcgaacgca atgccatttt agcttct
//
LOCUS       DAOSHV010000053         3533 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_24582_length_3533_cov_4.271708, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000053 DAOSHV010000000
VERSION     DAOSHV010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 3533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3533
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24582_length_3533_cov_4.271708"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1678)
                     /gene="ppsA"
                     /locus_tag="PKZ29_02300"
     CDS             complement(<1..1678)
                     /gene="ppsA"
                     /locus_tag="PKZ29_02300"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: protein motif:HMM:NF005057.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HOG37730.1"
                     /translation="MNKTANILWFNQISLEDVNLVGGKGGNLGEMYNLGIPIPNGFVV
                     TSDAYFQYIKENDLKPKIQDILKVTDVDQPDQLLTASKKIRSLIRKTPVSQNLSLEIM
                     KAYKKLSGFGGLKNIPVAVRTSATAEDSSDASFAGQGDTFLNVIGEVNVVNRVRECWS
                     SLFTSRSIFYQVKKNYDHFKIGVAVPVQKLINSEISGIMFTANPVTNAKDEIIIETIW
                     GLGEYIVQGKITPDQHLVDKKTWSIISQDHVSQTIQLTKDKNETKETNVPKTKVNRKK
                     IDDSMAIKIAKIGQKLHNHYAKPQDVEFAIDRGKLYIVQTRPITTLDTNQHQLESQIN
                     ITQDPILTGDPASPGLASGIVVIIKNPKEITRAQKGQILVTTMTTPDFVPAMKKVNGI
                     ITEKGGQTSHAAIVSRELSVPCVVGTKTATKELKEGDIVTINGTTGQIWRGDLIAGQK
                     VSTPNTKPVSNLKTATKVYVNLGEPDLAKTLSKRNVDGIGLLRAEFMMANIGVHPKYI
                     IAQNQQKKFIEKLAKNLLQFCKNFNPRPVIYRATDFKTNEYRYLKWGKLYE"
     gene            complement(1848..2252)
                     /locus_tag="PKZ29_02305"
     CDS             complement(1848..2252)
                     /locus_tag="PKZ29_02305"
                     /inference="COORDINATES: protein motif:HMM:NF013234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HOG37731.1"
                     /translation="MDKISHQQIIKKPWGQETIYTDEGSKYTFKQIAINDGHRLSLQS
                     HTDKSETFVLIEGQAELVIGNDIDHLETVPMEPKKGYTIPVGTIHRMVGVKNAVIMEA
                     STPETGTTIRLQDDYKRPDETENVRKLDNRGW"
     gene            complement(2273..2896)
                     /locus_tag="PKZ29_02310"
     CDS             complement(2273..2896)
                     /locus_tag="PKZ29_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37732.1"
                     /translation="MFYIFHGDHQLNSRQALNSFLDQKNDFDILRIDSKEINLDQING
                     FINSPSLFSKPKIIVFLNFFSIPKPILDKLKKIIETNPEFDIVIWQDKTIPPTQLKYF
                     SKARVETFTVDKIIFQCLNQLYPGNIKKFIPLFHRVLNQEPFELFLYWLKNHLRRQLT
                     VYSKFNQQRLKSTYIHIIELDYQSKTGQLAISKDTALEKILVSLLTK"
     gene            2957..3030
                     /locus_tag="PKZ29_02315"
     tRNA            2957..3030
                     /locus_tag="PKZ29_02315"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2991..2993,aa:Glu,seq:ttc)
     gene            complement(3033..3476)
                     /locus_tag="PKZ29_02320"
     CDS             complement(3033..3476)
                     /locus_tag="PKZ29_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37733.1"
                     /translation="MNQIQILFLILFGLISLITTIFSYFNIASKNNPFGLAPKFLNLF
                     TIFVWGDGLPIGLFLTLTTLISLHLNHWNLFLLFMSTFFLVRSVGETIYWLNEQFSSL
                     KRNPPKTLTGFKIYKNDSIWFAYQIFWQCLSAIFFVLSFYLLKKI"
BASE COUNT         1021 a          541 c          820 g         1151 t
ORIGIN      
        1 gttcgtagag ttttccccat ttgagatagc ggtattcatt agttttaaag tcggtagccc
       61 ggtagatcac tggccgggga ttaaaatttt tgcaaaattg aagtaaattt ttggcaagtt
      121 tttcgataaa ctttttttgc tgattttggg caataatgta tttgggatgg acaccaatgt
      181 tagccatcat aaattcagcc cggagcaagc cgataccatc aacgtttctt ttggataagg
      241 ttttggcgag atcgggttcg ccaagattga cataaacttt ggtggcagtt ttgagattgg
      301 agacgggttt agtgttagga gtagaaacct tttgaccggc tataaggtca cctcgccaga
      361 tttgaccggt ggtgccgttg atggtaacta tatccccttc tttgagttct ttggtggcag
      421 ttttggtgcc gaccacacac ggaacactta attcacggct gacgatggca gcatgagatg
      481 tttggccacc tttttcggta ataataccgt tgactttttt catcgccggg acaaaatcgg
      541 gagtagtcat ggtagtgact aggatttgac ctttttgggc ccgggtgatc tctttgggat
      601 ttttgataat gactacaatg cctgaggcta atccaggact ggcaggatcg ccggtaagaa
      661 ttgggtcttg ggtaatgttt atttgcgatt cgagttggtg ttggttggtg tcgagagtag
      721 tgataggacg ggtttgaaca atgtaaagtt tgcccctatc aatagcaaat tcaacatcct
      781 ggggtttggc ataatggttg tgaagttttt gaccaatttt ggcgatcttt atggccatag
      841 aatcatcaat tttttttcgg ttaactttgg ttttaggaac attggtttct ttggtttcgt
      901 ttttgtcttt agttaattga atggtttggc tgacatgatc ttgggaaata attgaccagg
      961 tttttttgtc aaccagatgt tggtctgggg taatttttcc ttggacaata tattctccca
     1021 gtccccaaat agtttcaatg atgatttcat ctttggcgtt ggtaaccgga ttagcagtaa
     1081 acataatgcc ggatatttca gagttaatta gtttttggac agggacggca actccgattt
     1141 tgaaatgatc gtaatttttt ttgacctgat aaaaaattga tctggaggta aaaaggctag
     1201 accaacactc ccgaacccga ttgacaacgt ttacttcgcc aataacattc aaaaaagtgt
     1261 ctccttggcc agcaaaggaa gcatcggaag agtcttcggc ggtggcagaa gttctcacag
     1321 caaccgggat gtttttgagt ccgccaaagc cggaaagttt tttgtaggcc ttcataattt
     1381 ctagggacaa attttggctg acgggggttt tgcggatcag actgcggatt tttttggaag
     1441 cggttaaaag ctggtcaggt tggtcgacat cagtaacttt tagaatatcc tgaattttgg
     1501 gtttaagatc gttttctttg atgtattgaa aataagcatc ggaggtgacg acgaagccgt
     1561 tggggatggg aatacctaaa ttatacattt ctcctaagtt tccacctttg ccgccaacta
     1621 agttaacatc ttcaagacta atttggttaa accaaaggat attggcagtt ttattcatat
     1681 aaaaatgata gcagaaattt ttgggagttg ttcgttagca gagcagtaga ttgattgcta
     1741 attgggggta attttgtgaa ttaacctatt tttagggata ttcaaacatt atcaaagtat
     1801 atacaaacat atttatatat acttatagct atcaaaatgt atcttgttta ccagccccgg
     1861 ttatctaatt tgcgaacatt ttcagtttcg tcaggacgtt tgtagtcatc ttgaagacgg
     1921 attgtggtgc cggtttctgg agtggaagcc tccataataa cagcattttt gaccccaacc
     1981 atgcggtgaa tagtgccaac ggggatggta tagccttttt tgggttccat aggaacagtt
     2041 tccagatggt cgatatcgtt gccaatgacc agttcggcct gaccttcgat aaggacaaag
     2101 gtttcgcttt tatcagtatg agattgaagc gagaggcggt gaccatcatt gatggcaatt
     2161 tgcttgaaag tatattttga accttcgtcg gtgtagatcg tttcctgacc ccagggtttt
     2221 ttgatgattt gttggtgaga gattttatcc atattttcca tgtctgattt tatcatttag
     2281 tcagcagact taccaaaatt ttttcaagag cagtatcttt actgatggct aattgtccag
     2341 ttttagactg ataatcaagt tcaattatat ggatataagt ggattttagt ctttgttggt
     2401 taaacttaga ataaacggta agctgtcggc ggagatgatt tttgagccaa tacaaaaata
     2461 gctcaaaggg ttcttgattt aaaactcggt ggaacaaggg aataaatttt tttatattgc
     2521 cggggtataa ctggttgagg cattgaaaaa taattttatc gacagtaaat gtttctactc
     2581 tagctttgga aaaatatttt agttgggtgg gaggaatggt tttgtcctgc caaataacaa
     2641 tgtcaaactc ggggttggtt tcaatgattt tttttagttt atctaagatt ggcttgggga
     2701 tagaaaaaaa atttaaaaaa acaattattt ttggtttgga aaaaagagag ggactgttga
     2761 taaagccatt gatttgatca agattaattt cttttgaatc tattcgtaga atatcaaaat
     2821 catttttttg gtcgagaaaa gaatttaggg cttggcggct attgagttga tggtcgccat
     2881 gaaagatata aaacatagct tagtttacca aagagataga aaatgagtta ataaactggt
     2941 agaatatcgt tactttggct ccgtcgtcta gtggcctagg actcctggct ttcatccagg
     3001 caatcgaggg ttcgaatccc tccggagtca cattaaattt ttttgagaag gtagaaactc
     3061 aagacaaaga aaatggcgga gagacattgc caaaagattt ggtaagcaaa ccaaatagaa
     3121 tcgtttttgt aaattttgaa gccggtgaga gttttgggtg gattgcgttt gagagaagaa
     3181 aattgttcgt taagccaata aatggtttcc cccacacttc tgaccaaaaa aaatgttgac
     3241 ataaaaagta aaaataaatt ccaatggttg agatggagtg aaattaaggt agtcagggtt
     3301 aaaaaaagac caattggcag tccgtcgccc caaacaaaga tggtaaataa atttagaaat
     3361 ttgggagcca atccaaaagg attatttttg gaggcaatat taaaataact aaaaatggtg
     3421 gtgatcaaag agattaagcc aaagagtatt agaaataaaa tttggatttg attcatgctt
     3481 aattatatca atagaataag gtagaattgg tagtggatgt caaaatagag cca
//
LOCUS       DAOSHV010000054         3530 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_24617_length_3530_cov_3.797986, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000054 DAOSHV010000000
VERSION     DAOSHV010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 3530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3530
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24617_length_3530_cov_3.797986"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            382..1425
                     /locus_tag="PKZ29_02325"
     CDS             382..1425
                     /locus_tag="PKZ29_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37734.1"
                     /translation="MSKKRCLYNFKCGVCGEYNQSKMPKYCRGCRAEFIYDGDSTVPT
                     QTIPYTETIIPDSDLTASEESAEVEEENIVKTNKKRFPWGWLFAVLIIVILGIVLVPP
                     VLDYIKSQTAANQAAIQRFAQPVNDQSQQQIRDLELKLAQTEGQSAALAQQNQTLTQQ
                     NQDLAIAAQQAEAQAAALVQQNQIAAAVQQPVQATVITYPTEPIIWANYNGQMTNFGI
                     FLDTELGRRITYTNRNLLVPDKAWNDPLTQAELRRVETTWVDVAIDLPEGMSATIFGH
                     DFQQGNVVLDKGVFINLTQPGHYEFRLKNGEVIIWYPGQEQHKANDWQRIRTEVENGN
                     FDIKHSLDLTTIR"
     gene            1549..2424
                     /locus_tag="PKZ29_02330"
     CDS             1549..2424
                     /locus_tag="PKZ29_02330"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HOG37735.1"
                     /translation="MTSKNKQPLVSVVMPVYNPGKYLVDAIDSILSQTFSNFEFIIVD
                     DASTDGSWKIIKSFAKKDSRIIAFKNKINLGVSLTSNIAISQARGKYIARMDSDDVST
                     PDRLQKQVDFLKNHPQIIVVGGQCAIVDENNQVVGFKKFPLTQNQIKDMIFWAVPIQQ
                     GYMMVNTSLLPKNFTWYHPSKFSAEEVDLYFNLFKYGDFANLPDNLYFYRKISTSLSH
                     QNPKKTFWITLKSRLNAVKNGFKPSPTAILINLIQIIVISLIPAKIIMDLWYLVRGVK
                     KPTGLTVGTFSQAQV"
     gene            2432..3193
                     /locus_tag="PKZ29_02335"
     CDS             2432..3193
                     /locus_tag="PKZ29_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37736.1"
                     /translation="MSFVKKHLYPIFLGLIVLFLCLQNFTPGTFLVGWDNLMPELGIW
                     LNLKRSFFAVWQAYQGLGLVGGMAHATDLIRQLILLPFTFILPVNLIRYFWHFAMIFL
                     GTFGIYFGLIKVAAFKKSTAFIAALFYLLNFGSIQNFWAPLETFSTFWGFFPWLIFSI
                     LDYLNHPTKLKLYKLLILNLLAIPAFYVQTVFIVYCLTLAIIFILHPKSFPVLVKIAH
                     QFLLAAASGLFFLYQSHPPYRRHWQFYVQRRNFFA"
     gene            3165..>3530
                     /locus_tag="PKZ29_02340"
     CDS             3165..>3530
                     /locus_tag="PKZ29_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37737.1"
                     /translation="MSNEETFLRNFARGKISDFLLLRGYYFDFPKNESYFMQPWRQHF
                     SLPLITPLAFIFGLMVIAGLIVLILKPRKKLFDKLILGLFLLSCLALLSQTPVFQQVN
                     QFIRQFSLIDQIFRSPFTKF"
BASE COUNT          925 a          887 c          624 g         1094 t
ORIGIN      
        1 cctcattttc cagtggaaaa attgatgatg agggttgatc tgaatgtttt tactttacaa
       61 acttgatgga ctttctactt tatgaactaa cgtatcctat aatcttgaca attaaacact
      121 tcgatgctat aatccccctg atactcaaaa agtatcaaac tttaacaaag tgaaaatgtt
      181 tatatcaaga caccgcgtct tgaccataaa gtttcacaga acttttacaa tttgcgagtt
      241 atccaaatgg gttttaccca ttttttacat atagttgctg ttaccaaacg acgactgggt
      301 tagggaagcg ccggcatgca ggcccatagt tttactaaag ggaagcaccg atgtgcaggc
      361 ccaaccgaaa ggagatcagg tatgtccaaa aagaggtgtc tctacaactt taagtgtggg
      421 gtttgcgggg aatacaacca aagcaagatg cccaagtatt gccgtggttg tcgagctgag
      481 ttcatctacg atggtgactc cactgtcccc acccaaacca ttccctacac tgagactatc
      541 atcccggact ccgacctgac ggcttctgaa gaatccgccg aggtggagga ggagaacatc
      601 gtaaaaacca acaaaaagag gttcccctgg ggctggctct ttgccgtcct catcattgtc
      661 attctgggta tcgtcctggt gccgcccgtc cttgactaca tcaagtctca gacggccgcc
      721 aatcaagccg ccatccagcg gtttgcccaa ccagtcaatg accagagcca acagcagatt
      781 cgagacctag aattgaaact ggcccagacc gaaggtcaaa gtgccgcatt ggctcagcaa
      841 aatcagactc tgactcaaca aaaccaggat ttggcaattg ctgcccaaca agctgaagct
      901 caagctgccg ctctggttca acagaaccag attgccgctg ctgttcagca accagtccag
      961 gcgactgtaa ttacctatcc gaccgaaccc atcatctggg ccaactacaa tggtcaaatg
     1021 accaactttg gtatcttttt ggatactgag ttgggacgga gaatcaccta taccaacagg
     1081 aatcttctag tgcccgacaa ggcttggaat gatccgctca cccaagctga actacggcgt
     1141 gttgagacta cctgggttga tgttgccatc gatctccccg aagggatgag tgctaccatc
     1201 tttggtcacg acttccagca agggaacgtg gttcttgata aaggtgtctt cattaatctg
     1261 acccagcccg gtcattatga attccgcctc aaaaacggcg aagtcatcat ctggtatccg
     1321 ggtcaagaac aacacaaagc caatgactgg caaagaatca gaaccgaagt tgagaacggc
     1381 aattttgata tcaaacactc cctcgatctg acaactattc gttaactcgc aaaccggccg
     1441 gaagaaggct cttttattca gcttctgctg tttaaaatcc ttcttcctgc caacctcatc
     1501 cgatgtgtat cggatctgat gagtccaaaa ggacgaaagg taaacactat gacatccaaa
     1561 aataaacaac cattagtttc tgtcgttatg ccggtttaca accccggtaa atacttggtt
     1621 gatgctatcg acagcatcct ttctcaaact ttttccaact tcgaatttat cattgttgat
     1681 gacgcctcta ccgatggttc ctggaaaatt atcaaatctt ttgccaaaaa agactcccgg
     1741 attatcgctt tcaaaaataa aattaacctt ggtgtcagtc tgacttccaa cattgccatt
     1801 tctcaggctc ggggcaaata tattgcccgg atggactccg atgatgtctc tacccccgac
     1861 cgtctccaaa aacaggttga ttttctcaaa aaccaccctc agattattgt tgttggtggt
     1921 caatgcgcta ttgttgacga aaacaatcag gtggttggtt tcaaaaaatt tcccctgacc
     1981 caaaaccaaa tcaaggatat gattttttgg gcggttccca tccaacaggg ttatatgatg
     2041 gtcaatactt cccttttgcc caaaaacttt acctggtatc acccttccaa attttccgcc
     2101 gaagaagtcg acctttattt taatctcttt aaatatggtg actttgccaa ccttcccgac
     2161 aatctttatt tttatcgcaa aatttctacc agtttgtctc accaaaatcc caaaaaaacc
     2221 ttttggatta ctctcaaaag tcgtcttaat gccgtcaaaa atggttttaa gcccagccca
     2281 accgctattt taatcaattt aattcaaatt atcgtcattt ctctgatccc tgccaaaatc
     2341 attatggatt tgtggtacct agttcgtggt gtcaaaaaac ccaccggtct tactgttggg
     2401 actttttccc aagctcaagt ataattaatc aatgagcttt gtcaaaaaac atttataccc
     2461 tatcttcttg ggtttaattg tcttattttt gtgtcttcaa aatttcactc ccggcacttt
     2521 tttggtcggt tgggacaatc tcatgcccga acttggcatt tggcttaacc tcaaaagatc
     2581 cttttttgcc gtctggcaag catatcaggg cttaggttta gtcggtggca tggcccacgc
     2641 caccgatctc atccgccaac ttattcttct cccttttacc tttattcttc ctgtcaatct
     2701 catccgctac ttttggcatt ttgccatgat ttttttgggc acttttggga tctattttgg
     2761 acttatcaaa gtcgctgctt tcaaaaagtc caccgccttt attgccgccc ttttttatct
     2821 cctcaatttt ggctctatcc aaaatttttg ggcgcctctg gaaacctttt ccaccttttg
     2881 gggctttttc ccctggctga ttttttctat tttagattat ctaaatcacc cgacaaagtt
     2941 aaaactttat aaacttttaa tcttaaattt acttgctatt cccgcctttt atgtccaaac
     3001 tgtttttatt gtctattgtc taactttagc cattattttt attctccacc ctaaatcttt
     3061 tcctgtttta gtcaaaatcg ctcatcaatt ccttttggct gctgcctctg gcttattttt
     3121 tctttaccaa tctcacccac cctaccgccg gcattggcaa ttttatgtcc aacgaagaaa
     3181 cttttttgcg taattttgcc cgaggcaaaa tcagcgattt tctcctcctt cgcggttatt
     3241 attttgattt tcccaaaaat gaatcttatt ttatgcagcc ctggcggcag cattttagct
     3301 tacccctcat cactcccttg gctttcattt ttgggcttat ggtcatcgct ggactcattg
     3361 ttcttattct caaaccccgc aaaaaacttt tcgacaaact cattcttgga ctatttcttc
     3421 tcagctgttt ggctctttta tcccaaaccc ccgtcttcca acaagtcaat caatttatcc
     3481 gtcaatttag tttaatcgac caaattttcc gctctccttt taccaaattt
//
LOCUS       DAOSHV010000055         3479 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_25110_length_3479_cov_3.830315, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000055 DAOSHV010000000
VERSION     DAOSHV010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 3479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3479
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25110_length_3479_cov_3.830315"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="PKZ29_02345"
     CDS             complement(<1..245)
                     /locus_tag="PKZ29_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37738.1"
                     /translation="MKRKIRLFLGLIMGGFFWQPMPVRAAECVETALGCVPYTTEGFV
                     SWLLQILFGISGGIAFLLMIYGFILMATSSGDEKKVQG"
     gene            281..1987
                     /locus_tag="PKZ29_02350"
     CDS             281..1987
                     /locus_tag="PKZ29_02350"
                     /inference="COORDINATES: protein motif:HMM:NF020075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HOG37739.1"
                     /translation="MRFNPPKLLFLLVLIFVGFWLIKPALADDLQNQIDQITKQISDL
                     ESAIAPLKTESSDLQKKISSAKSQITKIEGHISALNQNLADKETELNNQQKLLEARVR
                     RYYKNSKKFNPILMFLSSQEGTSLLQQYTWSQSIISQDKNIISQYSADIYSLNTNKTK
                     LESEKTKLANLKKNLESRFGFLAGEIKKAETYKAELSAKQQSLIAEKTAMFNTSVGDV
                     SSSDDPASRADYNPGFSPAFAAFSFGAPHRKGMSQYGAYGRAKSGQNHEAILKAYYGD
                     IRVEKIDTNGSIKTDKGTLPFEDNYLVGIAEMPARWGDNGGYEALKAQAIAARTYALS
                     YTNNLSGSICTTESCQVYSSARLNSPGRWKQAVEDTRGMVVKSNKTGNIFSTMYASTS
                     GGATYSYSSLSHSTPQVWDTTCGNQSCWPNDAYEKIAGSPWYYKGWYKTRSNNSYGRS
                     HPWLNQEEFADIVNAVLYFDKTGDSSHLSQIQNCIGSCDGNAWSKDELRRQVADKGGP
                     ISSINSVSVDYSTSGDTRTVHISTDKGQLNFSAANFKTVFNLRAPGALVIKSGLFNLE
                     KK"
     gene            2010..2711
                     /locus_tag="PKZ29_02355"
     CDS             2010..2711
                     /locus_tag="PKZ29_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HOG37740.1"
                     /translation="MNRSQDLDAIAPDDPIIPAKKSAPVKKKKPSVKSDSPAAEPDVY
                     GLLGHTQNSFSSVLVKPKILSFQEQDDGEKIYVGLRPHWINNLSWIVTFVFMIFLPIL
                     FKYIDILDIFPVSYVNFILVFWYLFTFIYAFEKFLSWYSNIFIITDQRIIDIDFKNIL
                     NKHFVQTRFAQIEDISSSIKGILGTFFNYGDVIIQTASEDNKIKFEKIPNPSKIIMVI
                     KDLQDLDRQKRGGAL"
     gene            2708..2935
                     /locus_tag="PKZ29_02360"
     CDS             2708..2935
                     /locus_tag="PKZ29_02360"
                     /inference="COORDINATES: protein motif:HMM:NF040135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37741.1"
                     /translation="MIFPQNQVLTSYFLSAVKVFLFILAAMFVFLSAIVVKQVASITK
                     SVSDKFNPTLTLISYIFLILTLILVLTVLTL"
     gene            2948..>3479
                     /locus_tag="PKZ29_02365"
     CDS             2948..>3479
                     /locus_tag="PKZ29_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37742.1"
                     /translation="MHFTCRSLIGKTTPGWWSQYWENEPDDDQKFTKGHLFGLISLES
                     LDAPELQDIGRDIIAKINQFYFSLESFSVTQNLRQTLSHIQQHPEFAHLKINITLLIV
                     VNQQSFFAALGDFKVILQRESKISQILAGSNQDITLVFGPIVGGDKLFIISQPFFDSL
                     GFDQIKNFLLDTRLSTI"
BASE COUNT          985 a          858 c          559 g         1077 t
ORIGIN      
        1 ccttgaacct ttttttcatc tcccgacgaa gtcgccatca atataaatcc ataaatcatc
       61 aacaaaaacg caattcctcc cgatattcca aacaaaatct gcagcagcca agaaacaaat
      121 ccttcggttg tatatggcac acaaccaaga gccgtctcta cacactcagc tgctctaacc
      181 ggcattggct gccaaaaaaa tcctcccatt atcagaccca aaaacaatct tatcttcctt
      241 ttcattatga ctccagtata tcaaatgcta cacttaaata gtgagattca atcctccaaa
      301 attactgttt ttactggttc ttatttttgt cggtttttgg ctaatcaagc cggctcttgc
      361 cgatgatctc caaaatcaga ttgatcagat taccaaacaa atttccgatc tggaatcagc
      421 tattgctcct ctcaaaaccg aatcttccga tctccaaaaa aagatttcct ccgccaaatc
      481 tcagattact aaaattgaag gtcacatttc tgccctcaac caaaacctgg ctgacaaaga
      541 aacggaacta aataaccagc aaaagctttt agaagccaga gtcaggcgct attacaaaaa
      601 cagcaaaaaa tttaatccca ttcttatgtt tttatcttcc caagagggga cttcgctact
      661 gcagcaatat acctggtctc aatccatcat cagtcaagac aaaaacataa tttcccaata
      721 tagtgccgat atttattctc ttaataccaa caaaaccaag cttgaatccg aaaaaaccaa
      781 attggctaat ctcaagaaaa acctagaatc ccgctttggg tttttagccg gcgagatcaa
      841 aaaagccgag acctacaaag ctgaactttc tgccaaacaa caatccttga ttgccgaaaa
      901 aactgccatg tttaatacct ctgttggcga cgtttcctct tctgatgatc ctgcttcccg
      961 agcagattac aacccgggat tttctccagc ttttgccgcc ttttcttttg gtgctcctca
     1021 ccgcaagggt atgtctcagt atggtgccta cggtcgggcc aaatcaggtc aaaatcatga
     1081 agctattctt aaagcctatt atggtgatat tagggttgaa aaaattgaca ccaacggctc
     1141 tatcaaaacc gacaagggca ccctgccttt tgaagataat tatcttgttg gtattgctga
     1201 aatgcccgcc cgttggggtg ataacggtgg ttatgaagct ctcaaagctc aggcaattgc
     1261 tgcccgcact tacgctctca gttacaccaa caatctctcc ggttctattt gcactaccga
     1321 atcctgccaa gtttatagtt ccgcccgtct caactcgccc ggccggtgga aacaggctgt
     1381 tgaagatact cggggtatgg tcgttaaaag caacaaaacc ggcaatattt tttctaccat
     1441 gtatgcttcc acttccggtg gcgccaccta ttcttatagt tctctgagcc attccactcc
     1501 tcaagtttgg gataccacct gcggcaatca gtcttgttgg cccaacgacg cctacgaaaa
     1561 aattgccggt tctccttggt attacaaagg ttggtataaa accagaagca acaattctta
     1621 tggtcgctcg cacccctggc tcaatcagga agaatttgcc gatattgtta atgctgtttt
     1681 atattttgac aaaaccggtg attccagcca cttgtctcaa attcaaaact gtattggctc
     1741 ttgtgatggc aatgcttggt ccaaagatga gctcaggcgt caggtcgccg acaaaggcgg
     1801 tccaatttct tccatcaact ctgtcagtgt tgactattcc accagcggtg atacccgaac
     1861 tgttcacatc tccaccgaca aaggtcagct aaatttttct gctgctaact tcaaaaccgt
     1921 cttcaatctt agagctccgg gagccttagt tatcaaatcc ggtcttttta atctcgaaaa
     1981 gaaataattt tttttgctaa actatagtta tgaatcgatc tcaagatttg gatgccattg
     2041 cccccgatga tcccatcatc cccgccaaaa aatctgctcc tgtcaaaaaa aagaaacctt
     2101 ctgtcaaatc tgattctcct gccgccgaac ctgatgttta tggacttctt ggacacaccc
     2161 aaaactcatt ttcttcagtt ttagtcaaac ccaaaatcct ttcttttcag gagcaagacg
     2221 atggcgaaaa aatatatgtt ggtctcagac ctcactggat taacaatctt tcctggattg
     2281 tcacttttgt ttttatgatt tttctcccca tcttgtttaa atatattgac atccttgata
     2341 tttttcccgt tagttacgtt aattttattt tagttttttg gtatcttttt acctttattt
     2401 atgccttcga aaagtttttg agctggtatt caaacatttt tattattacc gaccagcgca
     2461 ttattgatat tgatttcaaa aatattctca acaaacattt tgtccaaacc cggtttgccc
     2521 aaattgaaga tatttcttca tccatcaaag gtattcttgg cacctttttt aattatggcg
     2581 acgttattat ccaaaccgcc tccgaggaca acaaaattaa atttgaaaaa attcccaatc
     2641 ccagcaaaat cattatggtt atcaaagatc tccaggatct cgaccgtcaa aaacggggag
     2701 gagctttatg atttttcccc aaaaccaagt cctaaccagc tactttcttt ctgccgtcaa
     2761 agtttttctt tttattctgg cagccatgtt tgtttttctc tcagctattg ttgtcaaaca
     2821 ggtagcctcc attaccaaaa gtgtttctga taaatttaac cccaccctga ctcttatttc
     2881 ttatatcttt ttaattctca cccttatttt agttctgact gttttgactt tatagtatca
     2941 ttaattaatg cacttcactt gtcgctctct tattggcaaa accactcccg gctggtggtc
     3001 gcaatactgg gaaaatgaac ccgacgacga tcagaaattt accaaaggcc acctttttgg
     3061 cttaatcagc cttgagtcgc ttgatgctcc cgaactccag gatatcggtc gggatattat
     3121 tgccaaaata aatcagtttt atttttccct agaatctttt tccgtcaccc aaaatctccg
     3181 ccaaaccctg tcccatatcc aacaacaccc cgaatttgct cacctaaaaa taaacattac
     3241 cctattaata gttgttaacc aacaatcctt ttttgccgcc ctgggtgatt ttaaagttat
     3301 tctccaacgg gaatcaaaaa taagccagat tcttgccggc agcaaccagg acatcacttt
     3361 ggtttttggt cccattgttg gcggtgacaa gttatttatt atcagtcaac cgttttttga
     3421 tagtcttggt tttgaccaaa tcaaaaactt tcttcttgat acccgcttat ctactatcg
//
LOCUS       DAOSHV010000056         3445 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_25489_length_3445_cov_4.526549, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000056 DAOSHV010000000
VERSION     DAOSHV010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 3445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3445
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25489_length_3445_cov_4.526549"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="PKZ29_02370"
     CDS             <1..185
                     /locus_tag="PKZ29_02370"
                     /inference="COORDINATES: protein motif:HMM:NF039585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5915 domain-containing protein"
                     /protein_id="HOG37743.1"
                     /translation="DARDIVRQVQNLRKEMNLNLKDKIKIYLPDWPSDFETEIKSKTL
                     AESIIKADQLKIEKVS"
     gene            195..1193
                     /locus_tag="PKZ29_02375"
     CDS             195..1193
                     /locus_tag="PKZ29_02375"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOG37744.1"
                     /translation="MSKSPWLPVLISLVFLFAINILMVSSMAPALTKKQFIAWGIGLI
                     LFFIGRQINPKQISSSKWLIFLTSCALLLLPIILNSITRGSRRWIDLGFASIQPSEIV
                     KPWLLLFLGNTQFPLLHLIPVGIIAIQPDLGSAMSVLFLMMPVIIYNRKFLKISLIIA
                     AIFLTLSPLIYNFVLHDYQKERIVTFLNPSSDPLDKGYNVIQSKIAIGSGGFFGKGYK
                     KGTQGQLLFLPEKHTDFMFAATAEELGFVGISIILLAYYFLIKTLIDKAFLIRNNRIL
                     FLFTLGIALQIWMQVFVNIGMNIGILPVTGIPLPFLSVGGSSIMTLLFSIGIIYSS"
     gene            1232..1546
                     /gene="rplU"
                     /locus_tag="PKZ29_02380"
     CDS             1232..1546
                     /gene="rplU"
                     /locus_tag="PKZ29_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009429894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOG37745.1"
                     /translation="MKYAVIALSGSQFKITENQKLSINNLNLKEGSKNSTDQVLLVAD
                     DKNIKVGTPTVKDAKVEFTVLKNYKGKKVRVFRYKAKSKYRKTKGFRPQLTEIQISKI
                     TF"
     gene            1563..1814
                     /locus_tag="PKZ29_02385"
     CDS             1563..1814
                     /locus_tag="PKZ29_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOG37746.1"
                     /translation="MAHTKSQGKTNQKSNRPGQRRGVKKFGGQKAEVGQIIVRQVGSS
                     FRAGEGTKLGKDYTIYAIRPGLIKFFKRMGKRFVSVTKK"
     gene            1883..2734
                     /locus_tag="PKZ29_02390"
     CDS             1883..2734
                     /locus_tag="PKZ29_02390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOG37747.1"
                     /translation="MKQITQIPTNQLEPNPLQPRGIISPESVSELVESIKEHGILEPL
                     IIAKTPAGYQIIAGERRWRAARILKLEYVPAIIKQTNPQQMLEMAIVENVQRKDLNPI
                     ERAKAFLRLKDEFGFDNHQIAKKVSKSVPYVINCLKLLTLPDAIKDGLLSGLISEGHA
                     RALSSIGDTRLMIEAYKIVLKEKASVRRTEDIARRMKKKIEEGSVPITKENIPQFLKS
                     RLDNISLDMKNFFNDNTTHVKITQTNVATQVTFQFKGSVDQRFDNLKGLYKQICQKDL
                     SEQKTKN"
     gene            complement(2724..3314)
                     /gene="lepB"
                     /locus_tag="PKZ29_02395"
     CDS             complement(2724..3314)
                     /gene="lepB"
                     /locus_tag="PKZ29_02395"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOG37748.1"
                     /translation="MKNKYLSQGGFSLGNFLLEFIQSIVLALSVFVLLYLFVAQPNEV
                     KGSSMYPNFKDKEYLLTEKISYQLGNPERGDVVIFKAPPSEPCAADECEYIKRVIGVP
                     GDRVMVSEGDVYVNGNKLDQGFLPEPVYTSPGDYMQEGVERVVPDGQYLCFGDNRPNS
                     RDGREFGPINRKLIVGKAFMKYWPISAVEMVPKVSF"
BASE COUNT         1095 a          818 c          529 g         1003 t
ORIGIN      
        1 gtgacgccag agacattgtc agacaagtcc aaaacctacg caaagaaatg aatcttaatc
       61 taaaagataa aatcaaaata tatctgcccg attggccttc agattttgaa actgaaatca
      121 aatccaaaac cttagccgaa tctattatca aagctgatca actaaaaatc gaaaaagtca
      181 gctaaaattt attaatgtct aagtctcctt ggttaccggt tttaatcagc ctggtttttt
      241 tgtttgccat caacattctt atggtcagtt ctatggcgcc cgcccttacc aaaaaacaat
      301 ttattgcctg gggtattggt ttaatcttat ttttcatcgg tcgacagata aatcccaaac
      361 aaatttcttc ctccaaatgg cttatattcc ttaccagttg tgccttgctt ttactgccaa
      421 ttattctcaa ctccatcacc agaggttcac gccgatggat cgatcttggc tttgcttcca
      481 ttcaaccttc agaaatcgta aaaccctggt tactgctatt tttgggcaac actcagtttc
      541 ctcttcttca tcttatccct gtcggtatta ttgccatcca acctgattta ggtagtgcta
      601 tgtcggttct ttttctcatg atgccggtca ttatttacaa tcgaaaattt ttaaaaatat
      661 ctttaattat tgctgctata tttttgacct tgtcacctct gatctacaac tttgttctcc
      721 atgactacca aaaagaaaga attgtcactt ttctcaaccc ctcatccgat cctttagaca
      781 aaggctacaa tgtaattcag tcaaaaattg ccattggttc aggtgggttt tttggtaaag
      841 gctacaaaaa ggggacacaa ggacagcttt tatttcttcc cgaaaaacat actgatttta
      901 tgtttgccgc tactgccgaa gaacttggat ttgtcggtat ttctatcatt cttttggctt
      961 actattttct cataaaaacc cttatagaca aggctttttt gattcgcaac aatcggattt
     1021 tatttttatt taccttgggg attgccctcc aaatttggat gcaggtattt gtaaatattg
     1081 gtatgaacat cggcatcctt cccgttactg gtatcccttt gccctttctt tccgtcggcg
     1141 gctcttcaat tatgaccttg cttttttcta tcggcatcat ctactcttca taatcttgtc
     1201 aacctttttt cctgtgttat actacaaaag tatgaaatat gccgttattg ccctatctgg
     1261 ttctcagttc aaaattaccg aaaaccaaaa actatctatc aataatctca acctaaaaga
     1321 aggtagtaaa aatagcaccg accaggtttt gttagttgcc gacgataaaa atataaaagt
     1381 tggcactccc accgtcaaag acgccaaagt cgaattcacc gttctcaaaa actacaaggg
     1441 caaaaaagtt cgcgtcttta gatacaaagc caaatcaaaa taccgcaaaa ccaaaggctt
     1501 ccgaccgcaa cttaccgaaa ttcaaatttc aaaaattact ttttaatcac gattaacaac
     1561 tcatggcaca tacaaaatca caaggtaaaa ctaatcaaaa atcaaatcga cccggtcaaa
     1621 gacgcggtgt caaaaaattc ggcggtcaaa aagccgaagt cggacaaatt attgtccgcc
     1681 aagttggatc tagttttcgc gccggtgaag gcaccaaact gggcaaagac tacactatct
     1741 atgccatcag acccggtctg ataaaatttt tcaaacgaat gggaaaaagg tttgtctcag
     1801 tcaccaaaaa atagctaact gacttgcttt gacatagttt atcactcttt tttcacggat
     1861 gttttcatag taaccttttt ctatgaaaca aatcactcaa atacctacca accaacttga
     1921 acctaatccg cttcaacccc gcggcattat ttctcccgaa tcagtttccg aattggttga
     1981 atctataaaa gaacatggta ttttagagcc cctgattatt gccaaaactc ccgccggata
     2041 tcaaataatc gccggcgaac gtcgctggcg ggcggccaga attctcaaac tcgaatatgt
     2101 cccggctatc attaagcaaa ccaatcccca acaaatgttg gaaatggcta tagtcgaaaa
     2161 tgtccaaaga aaagatctta accctatcga aagagccaaa gcctttctcc ggctaaaaga
     2221 cgaattcggt tttgataatc atcaaatcgc caaaaaagta agcaaaagcg tcccttatgt
     2281 tattaactgt cttaaacttt tgaccctgcc agacgccatc aaagacggac tcctctccgg
     2341 acttattagc gaaggtcacg cccgagcttt gtctagcatt ggtgacaccc ggcttatgat
     2401 tgaagcttac aaaattgtcc tcaaagaaaa agcttcagtc aggcgaaccg aagacatcgc
     2461 tagaagaatg aagaaaaaaa ttgaagaagg ttcggttcct atcaccaaag aaaacatccc
     2521 ccaatttctc aaatccagat tagacaatat cagcttagat atgaaaaact tttttaacga
     2581 caacactact catgtaaaaa taacccaaac caacgtcgcc acccaagtca catttcaatt
     2641 caaaggtagc gttgatcaaa ggtttgacaa tctcaaaggc ctttacaaac aaatctgcca
     2701 aaaagacctg tccgagcaaa aaactaaaaa ctaaccttcg gcaccatttc caccgcactg
     2761 attggccaat acttcataaa cgctttccct acaatcaatt ttctattgat tggaccaaat
     2821 tcacgtccat ctctagaatt gggccgatta tcaccaaaac acaaatactg accatcagga
     2881 accacccttt ccaccccttc ctgcatataa tcacccggcg aggtataaac aggctctggt
     2941 aaaaaacctt gatccaattt atttccattt acataaacat ctccctcaga caccatcacc
     3001 ctgtccccag gaactccaat cactcttttt atatattcgc attcatcggc tgcgcacggt
     3061 tcagacggcg gcgccttaaa aatcaccaca tcacctcttt ccgggttacc aagctgataa
     3121 gatatttttt ccgtcaacaa atattcttta tccttaaaat taggatacat cgagctccct
     3181 ttaacttcgt ttggctgggc cacaaataag taaagcaaca caaaaacaga caaagccagc
     3241 acaatcgact gaataaattc aagcaaaaaa ttacccaaac taaaaccgcc ttgacttaaa
     3301 tacttgtttt tcatacttaa aattctaacc gatactgacc aaaaaagata tatttttgtt
     3361 tatgttttta aaactttcac caatctaggc tataataagc atattcggtt gtttagctca
     3421 gttggttaga gcgcttcgtt cacat
//
LOCUS       DAOSHV010000057         3401 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_25958_length_3401_cov_3.213987, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000057 DAOSHV010000000
VERSION     DAOSHV010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 3401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3401
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25958_length_3401_cov_3.213987"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1205
                     /locus_tag="PKZ29_02400"
     CDS             <1..1205
                     /locus_tag="PKZ29_02400"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HOG37749.1"
                     /translation="KSAPPQPKSFLFLIISLVIFLILSLFAFYSWTQTQARLRQSQSD
                     YQSLLDLKNNLDTSLASASAELNLVKSEDQFVKNNQLQSEVKDIKSGFSQALKLYEDI
                     VDLRDSRVRVSTIEPLFSSVLSDLSQFKYSQSLSKIATLSAKVTQERQKSLAVVGSVN
                     PDNVTENNTPPGSGFSRQKVKVGDSFFVVDIVAADLNSTRVIVDTASEGDCADNCPVL
                     PLGTYVSRNGAFAGINGSYFCPASYPSCASKKNSFDTLLMNKNKKYFNSDNNVYSTVP
                     AVIFSGASARFVTQSQQWGRDTGVDSVLANYPLLVFNKSLAAGDSSDTKLSAAGNRAF
                     IAAKGSTVYIGVVRGVSVVGAAKVLLEMGMDNALNLDSGGSTALWSGGYKYGPGRDIP
                     NAILFVRK"
     gene            1249..1824
                     /locus_tag="PKZ29_02405"
     CDS             1249..1824
                     /locus_tag="PKZ29_02405"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOG37750.1"
                     /translation="MPSSPCHQFDDQQLVHLSLTDSQYFLCLMQRYETKLTAYLNRFV
                     YLPSEDIADIIQESFINTYRHLGDYNPKLKFSSWLYRIVHNQAVNFLKKSRLTRQINF
                     DPEDDDFTSSLIAAVNIEQETVNQHFRRHVKTILQQLKPQYREVLVLKYFEDKDYHEI
                     SDILQKPMGTVATLISRAKHQFKQLYETNRF"
     gene            1805..2479
                     /locus_tag="PKZ29_02410"
     CDS             1805..2479
                     /locus_tag="PKZ29_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37751.1"
                     /translation="MKPTASNRLSASQKALQSIRQRRLQPLPKWHLYLKKSLIWLVVI
                     FFLLILSLGISLTLLGMIDNLFTPYFWFAAVAIFLFLSYFVLDQSRQAYRLETWQKVF
                     LISLSTFIFGFILFRRGFARRFDNHLETTVPYYRHLTPLKINTWSQPQNGLISGQITQ
                     ILSDTSFKLQDFTHQTWTIDYSTAVIRGRTQLVIGQEIKIIGRQTGSDSFVANEIRPW
                     IGKKSN"
     gene            2547..2912
                     /locus_tag="PKZ29_02415"
     CDS             2547..2912
                     /locus_tag="PKZ29_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37752.1"
                     /translation="MNTKTILSLSALVLGLAAVATPALAYQGDPTTKGPNYSPERHQI
                     NTTAFENNDYQSWLKNMTGRGATRFVNQANFKEFSQAQLAAQKGDNSLLTAFRAKYGM
                     GQRNGQGRGQGRGLHSAAR"
     gene            complement(2968..>3401)
                     /locus_tag="PKZ29_02420"
     CDS             complement(2968..>3401)
                     /locus_tag="PKZ29_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37753.1"
                     /translation="EQLEEEIDKNMNRVATQVQELIETVGAKGGIGTQVREIAQEQQK
                     LQQRIKEQYTSLSSRSNFAKLMIGADEEAIKELQQRLIENEQIINKLEKTKTATRVRA
                     DLEKIEETIDLMKYQNASLQSKIQLEQKYKGMFGWLLKLFR"
BASE COUNT          847 a          864 c          571 g         1119 t
ORIGIN      
        1 caaaatccgc cccaccccaa ccaaaatctt ttttattttt aataatttcc ttagttattt
       61 ttcttatcct tagtttgttt gctttttata gctggactca aacccaagcc cgtctccgtc
      121 aatcccaatc tgattaccag tctcttttag atttaaaaaa taatttagac acctcgcttg
      181 cttctgccag cgccgagctc aatttggtta aatccgaaga tcaatttgtc aaaaataatc
      241 aactccaatc ggaggttaaa gatatcaaat ccggcttctc ccaagccctc aaactttatg
      301 aagatattgt tgatttaagg gattctcggg ttagagtgtc tactattgaa cctcttttta
      361 gctcggttct ttccgacctt agtcaattta aatatagtca gtccctgtcc aaaattgcca
      421 ccctctctgc caaagtcacc caagagcgtc aaaaatcttt ggccgttgtt ggctctgtta
      481 atcccgacaa tgtcaccgaa aacaataccc cacccggcag cggtttttcc cgccaaaaag
      541 ttaaagtcgg tgatagtttt tttgttgtcg atattgttgc tgccgatctt aattccaccc
      601 gggttattgt cgataccgcc tccgagggcg attgcgccga caattgtcct gttttgcctc
      661 tgggcactta cgtttcccgc aatggcgctt ttgccggtat caatggttcc tatttttgcc
      721 ccgcctctta cccttcctgt gcctcaaaaa aaaattcctt tgacactctc ttgatgaaca
      781 aaaataaaaa atattttaat tccgacaata atgtttacag caccgttccg gctgttattt
      841 tttccggcgc ttccgcccgt tttgtcaccc aatcccagca atggggtcga gatactgggg
      901 ttgattcggt tttagccaat taccctcttt tagtttttaa caaaagttta gctgctggtg
      961 atagcagtga caccaaactt tccgccgctg gcaaccgcgc ttttattgcc gccaagggtt
     1021 ctaccgttta tatcggtgtt gtcaggggag tttctgtcgt cggggctgcc aaagttttat
     1081 tggaaatggg catggacaat gctctcaatc ttgatagcgg cggttccact gctctttggt
     1141 ccggcggtta taaatatggc cccggacgtg atattcccaa cgccatcctt ttcgtccgta
     1201 aataaatgaa agatttttta gttttcagtt gtattatatt aaaccaatat gccttcatcg
     1261 ccctgtcatc agtttgacga ccagcaatta gttcatctgt ctcttaccga cagtcaatat
     1321 tttctctgtc ttatgcagcg ctacgaaacc aaattaactg cctatcttaa ccgttttgtt
     1381 tatttaccat ccgaagatat cgccgatatt atccaagagt cgtttatcaa cacctatcgc
     1441 caccttggtg attacaatcc caaacttaaa ttttcctcct ggctttaccg tatcgttcac
     1501 aatcaggctg tcaattttct caaaaaatcc cgtctcaccc gtcaaattaa ttttgacccc
     1561 gaagatgacg actttacctc cagtctgata gctgccgtca atatcgaaca agaaactgtt
     1621 aaccaacatt tccgccgcca cgtcaaaaca attttacagc agctcaaacc tcaataccgc
     1681 gaagttttgg ttctaaaata ttttgaagac aaagattatc atgaaattag cgacatatta
     1741 caaaagccca tgggaaccgt cgccaccctt atttctcggg ccaaacatca gtttaaacaa
     1801 ctctatgaaa ccaaccgctt ctaaccgact gtctgccagt caaaaagctc tccaatctat
     1861 tcggcagcgt cggcttcagc ctcttcccaa atggcatctt tatctcaaaa aaagtttaat
     1921 ttggttagtt gtcattttct ttcttcttat tttgtctctt ggcatttctc tgactttgtt
     1981 gggaatgatt gataatttat ttactcctta tttttggttt gccgccgtgg ctattttttt
     2041 gtttctttct tatttcgttt tagaccaatc ccgtcaggcc tatcgtcttg aaacctggca
     2101 aaaagttttt ttaatttctc tctctacttt catttttgga ttcattcttt tccgccgggg
     2161 ttttgcccgc cgttttgata atcatctaga aaccaccgtc ccttattatc gccaccttac
     2221 ccccctcaaa attaacacct ggagccaacc ccaaaacggt ttaatttccg gtcaaatcac
     2281 tcaaatcctt tccgacacca gctttaaact tcaggatttt acccaccaaa cttggactat
     2341 tgattattcc accgccgtta tccgcggccg cacccagctt gtcatcggtc aggaaataaa
     2401 aattattggc cgccaaaccg ggtccgactc ttttgtcgcc aatgaaatcc gcccctggat
     2461 tggcaaaaaa tctaattgaa agaaattctc tttctcaggt gtattactag tagacatggc
     2521 ctgtcagatt aaaaattaaa tcaaatatga atactaaaac cattttgtct ttatcagctt
     2581 tagttttggg tttggctgca gttgccactc ccgccttggc ttatcagggt gaccccacca
     2641 ccaaaggtcc caactatagt cccgaaagac accaaatcaa tactaccgct tttgaaaaca
     2701 acgactacca gtcttggctt aaaaatatga ctggccgcgg tgctacccgg tttgttaacc
     2761 aagctaactt caaagagttt tctcaagctc agttggccgc ccaaaaaggg gacaactctc
     2821 ttttgactgc tttccgcgcc aaatacggta tgggtcaaag aaacggtcag ggtcggggac
     2881 aaggtcgggg tttgcactcc gccgcccgct aattttactc agaccaaaaa aaataacccc
     2941 cgtttggggg ttattttgtt tttaaaatta tctaaataat tttaacagcc agccaaacat
     3001 tcctttatat ttttgctcta actggatttt tgattgcaaa ctggcgtttt gatacttcat
     3061 caaatcaatc gtctcttcaa tcttttccaa atcagccctt actcttgtag ctgtcttggt
     3121 tttctccagt ttatttatga tctgttcatt ttctatcaat ctctgctgca gctcttttat
     3181 agcctcctca tcagctccaa tcatcagttt ggcaaaattg ctccggctgc ttaggctggt
     3241 atattgttcc ttaatccttt gttgcagttt ctgctgttct tgggcaattt ctctgacctg
     3301 tgttccaatt ccccccttag ccccaaccgt ctcaattaac tcctgaactt gagttgctac
     3361 tcggttcata tttttatcta tctcctcttc caattgttct t
//
LOCUS       DAOSHV010000058         3399 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_25983_length_3399_cov_6.259569, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000058 DAOSHV010000000
VERSION     DAOSHV010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3399
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25983_length_3399_cov_6.259569"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..>3399)
                     /locus_tag="PKZ29_02425"
     CDS             complement(24..>3399)
                     /locus_tag="PKZ29_02425"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF001616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOG37754.1"
                     /translation="LMPSVKRTYWGKKYPNIPKLDLIQIQKESWQDFLDRELKETIHA
                     ITPVVDYTGNNWQLDLGELSYDPITITPETAKKKGLNYTLPVRIKATLTNKRTGNVRE
                     EDVFFLNLPTMTDEGTFIINGIERGVINQLVRSPGVYFTGERDPATGKTLYNAELRPN
                     KGSWLEFFVGKKDVIFARIDRKKKFPATILLRAACQMNDKQLVSEFGDFISATIQADV
                     SKTPDEAVLEFYRKLRPGEPVVMENAQKFFEERFFDLRTYELGPVGRYKVNKKFGFKF
                     DDADKSNWVLRKEDLVATVKYLVQLQKGEVTRLDDIDSLANRRLRRCGEIVSQIAIRP
                     AMARFERMVKERMSLISTKEKPAPSQMINPQPLISTLNTFFRSNQLSAILDQTNPLSE
                     LDLLRRVTVVGIGGLTRDRAAFSIRDVHSSQYGRICTVRSPEGPNIGLVTYLALYAKV
                     DKYGFILAPFRKLVKVKNGYTISDDYFYLDADDEYDKKITHLGIEFDDQETTHQKWVP
                     IRYKGEFIEGPINQVEYIDLVPNQIVGASASLIPFVDHDDATRALMGSHMQTQAVPLV
                     SPQAPIVGTGMEAAVATSMNRSVLAHRSGKVTYVDGSKIVITLDKKAPVEYHDESEIS
                     ADGKTETYYLSKFERTSPYGTCYNQKSVVKTGDMVKQGDLLIDGPSTDGGELSIGKNL
                     LVAYAELDGLNYEDSFLISDRLVKEDILTNIQIYEYQAQVVETKLGSEELTKDIPNVS
                     ENELSKLTDDGIVMIGATVGPNDILVGKVEPRGEKELSAEERLLRAIFGEKAREVKDT
                     SLRVANGEGGVVIKVDVLDKDQGDELDPGVNKVVKVYVAQIRRIQEGDKIAGRHGNKG
                     VIGRVWPSYDMPRTADGTPVDLVMSPISVISRMNLGQILETNLAHAGLYLNKRYAVPV
                     FEKYPESRITSELEAAGLNKNCKVQLYDGRTGEAYDQQTVVGVGYILKLVHMVDDKVH
                     SRSTGPYSLVTQQPLGGKARMGGQRLGEMEVWALEAHRAAHTLQEMLTIKSDDIDGRT
                     HAFQSIIKGEPIPEPAIPESFKVLTKELAGLALDISPVGVKEIDILPEDDTPPADFKP
                     EDISLEIPAEENFEAESDGLTVADSAKLEDETKEEQ"
BASE COUNT          946 a          700 c          782 g          971 t
ORIGIN      
        1 gatcatattt ttaaatttaa acattattgt tcctcctttg tttcatcctc taacttggct
       61 gaatcagcaa cagttaaacc gtcactttca gcttcaaaat tttcttctgc cggaatttct
      121 aaactaatat cctccggctt aaagtcggcg ggtggagtgt catcttcggg taagatatca
      181 atctccttga caccgacggg cgaaatatcg agagctaaac cggcaagttc tttggtcaga
      241 actttgaatg actcaggaat agctggctcc gggataggtt cgcctttgat aattgactga
      301 aaagcgtgag ttctgccatc aatatcatca gatttgatgg ttaacatttc ttgaagagta
      361 tgggcagccc gatgagcttc aagagcccaa acttccattt ctcccaatct ttgacctccc
      421 atccgtgctt taccacccaa gggttgttga gtaaccaaag aataaggacc ggtggagcgg
      481 gaatggactt tatcatcaac catatgaact agtttgagaa tatagcctac accaacgaca
      541 gtttgttgat cgtaagcctc tccggtgcga ccgtcataaa gctggacttt gcaatttttg
      601 tttagaccgg cagcttcaag ttcgctagta atccggcttt cggggtattt ttcgaagact
      661 ggcacggcat atcttttgtt gagataaaga ccggcatgag ccaaattggt ttccaaaatt
      721 tgacctaagt tcatccggga aataacggaa ataggcgaca tgactaaatc aacaggagtg
      781 ccgtcggcgg tgcgaggcat atcatatgaa ggccagacac gaccaataac acctttgtta
      841 ccatgtctgc cggcaatttt gtctccttcc tgaatccggc ggatttgagc tacataaact
      901 ttgacaactt tgttgacacc gggatcaagt tcatcaccct ggtctttgtc taaaacatcg
      961 actttgataa cgacaccgcc ttcaccgtta gcaaccctaa gtgaggtgtc tttgacctct
     1021 ctggcttttt caccaaaaat agctcgaagc agccgttctt cggcagataa ttctttttcg
     1081 ccgcgaggtt cgaccttgcc gaccaaaata tcattgggac cgacagtggc gccgatcata
     1141 acaataccgt catcggtgag tttgctgagt tcgttttcgg aaacattggg aatatctttg
     1201 gttaattctt cggaaccaag tttggtttcg accacttggg cttgatattc ataaatttga
     1261 atgttggtta aaatatcttc tttaactaat ctgtcagaga tcaaaaatga gtcttcgtag
     1321 ttgagaccat caagttcagc gtaagcaaca agcaaatttt tgccaataga taattctcca
     1381 ccgtcagtgg aaggaccatc aatcaaaagg tcgccttgtt taaccatgtc tccggtcttg
     1441 acgacagatt tttggttgta acaagtgccg tagggcgaag tgcgttcaaa tttggataaa
     1501 taataggttt cggttttacc gtcagcgctg atttcacttt cgtcgtgata ttcaaccggg
     1561 gcttttttat cgagagtaat aactattttt gaaccatcga cataagtgac tttgccggaa
     1621 cggtgggcta aaacgctgcg gttcatggaa gtggcgacag cggcttccat accggtgccg
     1681 acaattgggg cctgaggaga aaccaaagga acagcttgag tctgcatgtg ggaacccata
     1741 agagcccgag tggcgtcgtc atgatcaaca aaaggaataa gagaagcgga agcaccgaca
     1801 atttgattgg ggaccaaatc aatatactcg acttggttta tcgggccttc aataaattct
     1861 cccttgtaac ggataggaac ccatttttga tgagtggttt cctgatcatc aaattctata
     1921 ccaagatggg taattttttt atcatattca tcgtcggcat caagataaaa ataatcatcg
     1981 gagatagtgt agccgttttt gactttgact aattttctga agggagccaa aataaagcca
     2041 tatttatcga ctttggcata taaggccagg taagtgacta aaccaatgtt ggggccttca
     2101 ggtgaacgca cagtgcaaat acgaccgtat tgagatgaat ggacgtcgcg gatagagaaa
     2161 gctgcccgat cacgagttaa accgccgata ccgacaacgg tgacacggcg aagcaaatca
     2221 agttctgata aaggattggt ttggtcaaga atagcggaca attgattgga tctaaaaaaa
     2281 gtattgagag tggaaataag cggttgaggg ttgatcattt ggctgggagc gggtttttct
     2341 ttggtagaaa ttaaactcat tctttctttg accattcttt cgaatcgagc catggccggg
     2401 cggatggcaa tttgagacac aatctctcca caacggcgga gacggcgatt agccaagctg
     2461 tcgatgtcgt ccagacgggt aacttcacct ttttggagtt gaacaaggta tttaacggtg
     2521 gcaactaaat cttctttgcg caggacccaa ttggatttgt cagcatcgtc aaacttaaaa
     2581 ccaaattttt tgttgacttt gtaacgacct accggaccta attcgtaggt tctcaggtcg
     2641 aaaaatcttt cttcaaaaaa tttttgggcg ttttccataa cgaccggctc accgggacgg
     2701 agtttacggt aaaactccaa aacagcttcg tcgggggttt tggaaacgtc ggcttgaatg
     2761 gtggcagaga taaaatcacc aaactcgctg actaactgtt tatcattcat ttggcaagca
     2821 gcccggagaa gaatggtggc aggaaatttc ttttttcggt cgatacgggc aaaaattaca
     2881 tcttttttac cgacaaaaaa ttcaagccaa gaacccttgt tggggcgaag ctcggcgttg
     2941 taaagggttt tgccggtagc tggatctctt tcgccagtaa aataaacacc gggagatcgg
     3001 actaattggt tgataactcc cctttcgata ccgttgataa tgaaagtgcc ttcatcggtc
     3061 atggtaggca aattgagaaa gaaaacatct tcttcgcgaa cattgccggt gcgtttgttg
     3121 gtaagggtgg ctttgatacg gacaggaagg gtgtaattta aacctttttt cttggcagtt
     3181 tcaggagtaa tagtgatagg atcgtaggaa agttcaccca aatcaagctg ccaattgttg
     3241 ccagtgtaat caactaccgg agtgatggca tgaatagttt ctttaagctc acgatcaagg
     3301 aaatcctgcc aagattcttt ttggatttga attaaatcta atttgggaat attgggatac
     3361 tttttacccc agtaagttcg ttttacggaa ggcatcaat
//
LOCUS       DAOSHV010000059         3277 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_27395_length_3277_cov_5.243327, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000059 DAOSHV010000000
VERSION     DAOSHV010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27395_length_3277_cov_5.243327"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1385)
                     /locus_tag="PKZ29_02430"
     CDS             complement(<1..1385)
                     /locus_tag="PKZ29_02430"
                     /inference="COORDINATES: protein motif:HMM:NF015448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HOG37755.1"
                     /translation="MKLIYSHLQKFLPDLKVDPQQLRDDLTMIGHFTNYFEEIDHQIV
                     FDLDIKVNRGDCLGYYGLARDLAVLYHLPLNDLTATGKLNLQSLPALPITITTDKVKR
                     VMAVKISRIHNQISPPWLQTFIKLHGTNPVNLAVDLTNYIMFMYGIPNHAFDIRKSGQ
                     NLIWEINPKYTEFTSLDGSHLKLDSHILMINNPSHALSLSFWGGEACAIDLDTDEIIL
                     EMAIYDPATVRQNSRFLKSTTEAGTRLEKNLDPDTIPQAFTDLINLILEHCRGQISSQ
                     VFDYYPQKNKPKKIKFDLNQPSQISGINIPSDFALDVFARLGCLIDTHPSLSIAPPSI
                     RPDISISADLVEEIVRFFGYQHIPLDQPLTPKQLPDITPAQVYLSEKLKDNLINLGYD
                     EILTWPLTDTFLNKKTVVKTQNSINTEAVYLRQSLIPSLKHQLDQYQRFKLPPTQFFE
                     IGKTFFKKGNH"
     gene            complement(1382..2395)
                     /locus_tag="PKZ29_02435"
     CDS             complement(1382..2395)
                     /locus_tag="PKZ29_02435"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF013568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOG37756.1"
                     /translation="MDQDLASIKQKYSQQIFNLDNSQDLEKIRIELFGRHGLFNQLFT
                     KIKDIPSDQRKQYGANLNQTKSELETILNQKNISPHQTSTATKINLPLFALPKIGHLH
                     PITQTERQLNALFKSLGFSVYTSPEIVTDEYNFTRLNVPTNHPARDMQDSIYIKEPEY
                     LLRTQTSTIEAYLLQAQSQQLPLRAAFPGAVFRNEKVNRSNHFVFHQYQAVVVDKNIT
                     MKDLIGTMELMFRTLYGPSAIVRYRCKYYPEVEPGVGPDMQCFSCQGKGCPLCKYAGW
                     IEMGGSGMIHPQVLKKAGIDPKIWSGFAFGMGLDRWTMAQNKISDIRTLMGGNLAYKP
                     NQP"
     gene            complement(2459..2800)
                     /gene="rplT"
                     /locus_tag="PKZ29_02440"
     CDS             complement(2459..2800)
                     /gene="rplT"
                     /locus_tag="PKZ29_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015902141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOG37757.1"
                     /translation="MRVKTGTVRRAAHKKVLKAAKGFWMTRHKRYKVAHEAVMHAGDY
                     AFAGRRLKKRDLRQVWIVRINAALKPFDIRYSAFIKMLKNRNIAIDRKILADLAVNNP
                     DAFKAVVESAK"
     gene            complement(2860..3069)
                     /gene="rpmI"
                     /locus_tag="PKZ29_02445"
     CDS             complement(2860..3069)
                     /gene="rpmI"
                     /locus_tag="PKZ29_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003970213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOG37758.1"
                     /translation="MKKTSKKLKVRKSVSNRFEVTKTGKVLRMSSFNRHLRRKKSKKQ
                     LRRLKGKQVVEGALAIRVKKLLGHL"
     gene            complement(3038..>3277)
                     /locus_tag="PKZ29_02450"
     CDS             complement(3038..>3277)
                     /locus_tag="PKZ29_02450"
                     /inference="COORDINATES: protein motif:HMM:NF012910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37759.1"
                     /translation="KKFLATGNKIKLSIKFQGRQMTQQQFGHNLVAKFKSDLQDFATA
                     EGEAKLIGKRLMITFTPSKKKAAPTHEENIEKTKS"
BASE COUNT          921 a          520 c          812 g         1024 t
ORIGIN      
        1 taatgattac cttttttgaa aaatgttttt ccgatctcaa aaaattgggt cggaggcagt
       61 ttaaagcgtt ggtattggtc aagttgatgt ttgagtgagg gaatgagaga ttggcggagg
      121 taaacagcct cagtgttgat gctgttttga gttttgacaa cagttttttt attcaaaaag
      181 gtatctgtca gcggccaagt taggatttcg tcataaccaa gattgattaa attgtctttt
      241 aatttttcgg agagataaac ttgggcagga gtaatatcag ggagttgttt gggagtcaac
      301 ggctgatcga gaggaatatg ctggtagcca aaaaatctaa cgatttcttc taccagatcg
      361 gcagaaatag agatatcggg acggatagac ggtggggcga tggagagtga gggatgtgtg
      421 tcaataagac agccaagacg ggcaaagacg tctaaagcaa agtcagaagg gatgttgata
      481 cctgatattt gactgggttg atttaggtca aatttaattt ttttgggttt atttttttgg
      541 ggatagtaat caaatacttg ggaagaaatt tggccacgac agtgttctaa aataaggttg
      601 attaaatcag taaaagcttg aggaatagtg tcggggtcga gatttttttc taatctggta
      661 ccggcttcgg tggttgattt gagaaatcgg ctgttttgac ggacggtggc agggtcatag
      721 atagccattt cgagaataat ttcgtcggta tctaaatcaa tagcgcaggc ttccccaccc
      781 caaaaactca agctgagggc gtgactgggg ttgttaatca taagaatgtg agagtctaat
      841 ttgagatgag aaccgtcgag actggtaaat tcggtatatt tgggatttat ttcccaaatc
      901 aaattttgac cggacttgcg gatatcaaaa gcgtgattgg gaataccata cataaacata
      961 atatagttgg tcaggtcgac ggctaagtta actggattgg tgccgtgaag cttgataaaa
     1021 gtctggagcc atggaggcga tatttggttg tgaatacggg aaattttaac agccataacc
     1081 cgtttgactt tgtcggtggt gatagtgata ggtagagccg gcaaagactg aagatttaat
     1141 ttgccagtgg cagttagatc atttagaggg agatgatata aaacagcaag atcgcgggcg
     1201 agaccataat atcccaggca gtctccccga ttgactttga tatctaaatc aaaaacgatt
     1261 tggtgatcaa tttcttcaaa ataattagta aaatggccga tcatggtcag gtcatcacgg
     1321 agttgttggg gatcaacttt gagatcggga agaaattttt ggagatgaga atagattagt
     1381 ttcatggttg gttgggtttg taggctaaat ttcctcccat aagggtgcga atgtcactaa
     1441 ttttgttttg ggccatggtc catcggtcaa gtcccatacc aaaagcaaat ccggaccaaa
     1501 tttttggatc aataccggct tttttgagaa cttgagggtg aatcataccg ctgccgccca
     1561 tttcaatcca accggcatat ttacacaaag gacaaccttt accttggcag ctgaagcact
     1621 gcatgtcggg gccgacaccg ggttcaactt cgggataata tttgcaacga tatctgacga
     1681 tggcacttgg tccgtataaa gtgcggaaca ttaattccat ggtgccgatc aagtctttca
     1741 tggtaatatt tttgtcgaca acaacagcct gatattggtg aaagacaaaa tggtttgaac
     1801 ggttaacttt ttcgtttcta aagacggcgc cggggaaggc agcccggaga ggcaactgtt
     1861 gggattgggc ttgaagcaaa taagcttcga tagtagaggt ttgggttcgg agaagatatt
     1921 ctggctcttt gatataaata ctatcctgca tatcgcgggc gggatgattg gtggggacat
     1981 tgagacgggt aaaattgtac tcatcggtaa caatttccgg gctggtgtag acagaaaagc
     2041 caagagactt aaaaagagcg tttaattgac gttcggtttg ggtaatggga tgaagatgac
     2101 cgattttggg aagagcaaat aaagggagat tgattttggt ggcggttgaa gtttgatgag
     2161 ggctgatatt tttttgattg agaatagttt ctaattccga tttagtctga ttgagattgg
     2221 cgccgtattg ttttctttgg tctgaaggaa tatctttgat tttggtaaaa agttgattaa
     2281 acagtccatg acgaccaaaa agttcaattc ttattttctc caggtcctga gagttgtcca
     2341 aattaaatat ttgttgggaa tatttttgtt tgatggatgc caaatcttga tccatagatg
     2401 actttatttt aacaaaaaac cccgcagtgg cggggttttg gttgaattaa gtaaaatatt
     2461 atttggccga ttcaaccaca gccttaaagg catcggggtt gttgacggct aggtcggcca
     2521 aaattttgcg atcaatagca atatttctat ttttaagcat cttgataaag gctgaatagc
     2581 gaatatcaaa tggttttaga gccgcattga ttctaacaat ccaaacttgt cggagatccc
     2641 gttttttgag acgacgacca gcaaaagcat aatcgccggc gtgcataaca gcctcgtggg
     2701 caaccttgta acgtttgtgt cgggtcatcc aaaagccttt ggcagctttg aggacctttt
     2761 tgtgagcagc tcttctgact gttccggttt taactctcat agttagttta taaagtttaa
     2821 agttataaag tttatcaagt tagttgataa taataaagat tagagatgac ctaaaagttt
     2881 tttgactctg atagctaaag ctccttcgac cacttgttta ccttttaggc ggcgaagttg
     2941 ttttttgctt tttttgcggc gtaaatgtcg gttaaaggag gacattctca agaccttacc
     3001 ggttttggtg acttcaaacc gattagagac agattttcta acttttagtt ttttcgatgt
     3061 tttcttcatg agtaggagca gccttctttt ttgaaggagt aaaagtaatc attaatcttt
     3121 tgcctataag tttggcttcg ccttcggcgg tggcaaaatc ctggagatca gatttgaatt
     3181 tggctaccag gttgtgtcca aattgctgtt gagtcatctg acgaccttgg aattttatac
     3241 ttaatttgat tttatttcca gtagctaaaa atttttt
//
LOCUS       DAOSHV010000060         3272 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_27451_length_3272_cov_3.980417, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000060 DAOSHV010000000
VERSION     DAOSHV010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 3272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3272
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27451_length_3272_cov_3.980417"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="PKZ29_02455"
     CDS             <1..189
                     /locus_tag="PKZ29_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37760.1"
                     /translation="QLKYHPICSIDVDFGEEPSAWAAFVDVDPTAGTIQNFCVDSSGF
                     SGIGYMVGESTGKCIEQF"
     gene            331..2283
                     /locus_tag="PKZ29_02460"
     CDS             331..2283
                     /locus_tag="PKZ29_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37761.1"
                     /translation="MNKKTFLGLVFFSVVIMVAMINWPYSVAPVGAQSTSTEDNELQG
                     WAWSDSTGWVSFNHKQFNEEITPATTGSDDTGWKTPGLATQKVKDPNSLNWNIFGITN
                     LTNLIAMSESVLRDVRNTNYLETRDFGFNIPSDANITGIEVCYLRTGGARKIDKVNGD
                     TLYRVYDEDIRLIKPNGSYTQDNKSKTEPWELSPVPSDTFYEACYGGANDTWGENWTS
                     SDIVNPNFGAVMSAYLPAEDYLSGATVKTVKVKVFYKQTFGAYSTSNNSNYAVKVDHT
                     GNLTGYAWSLNLGWIKFAPELYPTGVCGATNDCYGAKIVEGGTIDEVKGWARVCSVYQ
                     SGCSGTLRSDAELGGWDGWIRMHNVKRQANGGYFGYAWGDLNLAWLNFGFGFSSDDGG
                     PDDGTCDPSKQVCGGIVVACDSEQQCCDGDTYPENLNCGREATISVNGQCVGEALPFF
                     DPDGADCGPYCRTYPVNQTVKVGFQGLEPGTISSWDSNGDPCVDNGDNSCTIAQLTKN
                     TGLTANCVQTPLNECSVMISSGTMSLVEGKNLDYTEGVLSNAAKVVLKTVDGTPCHGT
                     YNLESSILDNSAGLSILCLKNGTYGDDCTGLDGASETLVKAKFTARPEISPSFWNVTM
                     WATRDEESSQPKTYKNLIRYQRKAAN"
     gene            2289..3083
                     /locus_tag="PKZ29_02465"
     CDS             2289..3083
                     /locus_tag="PKZ29_02465"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HOG37762.1"
                     /translation="MSPLLCLIFLIFGLIIGSFLNVIVLRLHSGESVVFGRSRCLSCS
                     KKLNWPELIPVLSFLVQRGHCRGCHSRISWQYPLVELLSGVLFVSVYLRWAADGWLTL
                     LFYLIIVSLFIATTVYDLRHKIIPDLFVSLLVVLALLRPLFFSLVSHDFHSLISDYQT
                     VIWGGLAAGGVFFLLWLLSRGRWLGFGDVKLALAIGFLLGPVQVFSALVLATWFGAIV
                     GLVLVILTKTGLFGFRKYFTIKSELPFAPFIILGVLLNLLFQVNVF"
     gene            complement(3051..>3272)
                     /locus_tag="PKZ29_02470"
     CDS             complement(3051..>3272)
                     /locus_tag="PKZ29_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37763.1"
                     /translation="ANYFFSRAINLGCSYATGYFLRGQVKRPLATKIGISAITTAVII
                     KKIVITTINSSSEKPCCDFLKDIDLEKQI"
BASE COUNT          824 a          621 c          829 g          998 t
ORIGIN      
        1 caattaaaat atcacccgat ttgttctatt gatgtagatt ttggagagga accttctgct
       61 tgggccgctt ttgttgatgt tgacccaaca gccggaacaa ttcaaaactt ctgcgttgat
      121 agcagtggct tttccggaat aggctatatg gtgggggaat ctactggcaa atgtatagaa
      181 cagttctaga cactcccagt cctcgtttta ggcttaaagt ggataacttt tgacaaaaat
      241 agctgggttt tgtgttaaaa ttgaggtaat ttttaaggta aaaaattaat taaaatatgc
      301 ataaatttat atttttaaaa ctatcaaact atgaataaaa aaactttcct cggtctggta
      361 ttttttagcg tcgtgattat ggtcgcgatg atcaactggc cttattcggt ggccccggtc
      421 ggcgcccaaa gcaccagcac ggaagataac gaattgcagg gctgggcttg gagtgacagt
      481 accggttggg taagttttaa ccataagcaa tttaacgaag aaattacccc agccacgacc
      541 ggctcggatg acacggggtg gaaaacacca ggtttggcta ctcaaaaagt aaaagaccct
      601 aactctttaa actggaacat ttttggtatc actaatctaa ctaatttaat tgctatgtcg
      661 gagtcggtgt tgcgagatgt gcggaacact aattatttgg aaactaggga ttttggcttc
      721 aatatccctt ctgacgcgaa tataactgga attgaggtgt gttatctgcg aacaggcggg
      781 gcgcgcaaaa ttgataaggt caatggggat acgctttata gagtttatga tgaagatatt
      841 agattaataa aaccaaatgg ctcgtacact caagacaaca aatctaaaac tgagccgtgg
      901 gagcttagtc ctgtcccatc agatacattt tacgaggctt gttacggagg tgccaatgac
      961 acttggggcg agaattggac ttcgtcggac attgtcaacc ctaactttgg tgcagtgatg
     1021 tcagcttatt tgccggcgga agactacctt agcggtgcta ctgtgaaaac tgtaaaggtc
     1081 aaagtttttt ataaacaaac attcggcgct tattcaacca gcaataattc aaattatgcg
     1141 gtaaaggtag atcatacggg aaatttaacc ggttatgcct ggagcttgaa tttgggctgg
     1201 attaagttcg cgccggaatt gtatccaact ggtgtttgtg gggcaaccaa tgattgttac
     1261 ggcgccaaaa ttgttgaggg cggaacaata gacgaggtca agggttgggc aagggtttgt
     1321 tcggtttacc aaagtggctg tagtggcacc ttgcggtcgg atgcagaact cggcggttgg
     1381 gatggttgga ttagaatgca caatgttaag cgtcaagcga atggcggtta ctttggctat
     1441 gcttggggcg acctgaacct agcgtggcta aactttggct ttgggtttag cagtgatgat
     1501 ggcggtcctg atgatggcac ttgcgacccc tccaagcagg tttgtggggg aatagtagtt
     1561 gcgtgtgact cagagcaaca atgttgcgat ggagatactt atcccgaaaa tctaaattgc
     1621 ggccgagaag cgacgattag tgttaatggt caatgcgtgg gagaggcgct accattcttt
     1681 gatccagacg gtgccgattg tggtccctac tgccgaactt atccggtcaa tcaaacggtt
     1741 aaggtagggt tccagggttt agaaccgggg accatttcaa gttgggattc taacggggac
     1801 ccatgtgttg ataacggcga taactcttgc acaatagcac aattgactaa aaatacaggt
     1861 ttgaccgcca attgcgttca aacgccgctt aacgaatgtt ctgttatgat atcgagcgga
     1921 accatgagct tggtagaggg caagaatctt gattataccg agggcgtctt gagtaacgcg
     1981 gcaaaagttg tcttaaaaac agttgatggc acaccctgcc atggaacgta taacctagag
     2041 agttctattt tagataattc agccggccta tctattcttt gcctcaaaaa tggaacttat
     2101 ggtgatgatt gcactggctt agacggcgca agtgagactc tcgtaaaagc aaagtttaca
     2161 gcaaggccgg aaatttcccc tagcttttgg aatgtaacca tgtgggcgac cagagacgaa
     2221 gaatcttccc agcccaagac ttacaaaaat ctaattaggt atcagagaaa agctgctaac
     2281 taaattcaat gtccccttta ttatgtttga tttttcttat ctttggcctg attatcggta
     2341 gctttttgaa cgtaattgtc ttgcgcttgc actctggcga gtcggtggtt tttgggcgct
     2401 cacgctgtct ttcttgtagc aaaaaactta attggcccga attaattcca gtgcttagtt
     2461 tcttggtcca aaggggccat tgtcggggtt gtcactcaag aatttcttgg caatatccgt
     2521 tggtggaact gttatcgggt gtgttgtttg tttcagttta tcttcggtgg gcggctgatg
     2581 gttggctgac cttactcttt tatctgataa tcgtgtcgtt gtttattgcc acaactgttt
     2641 atgacttaag acataaaata attcccgatt tgtttgtttc tctcttggtt gttctggctt
     2701 tgctcaggcc attgtttttt tcgttggtta gtcacgattt tcactcttta atttctgatt
     2761 atcagacagt catttggggc ggtctagcgg ctggcggcgt cttcttcttg ctatggcttt
     2821 tgtcgcgcgg tcgctggctc ggttttggcg atgtcaaact ggctttggcg atcggttttc
     2881 tgcttgggcc ggtccaagtt ttttcggctc tggtgctggc gacgtggttt ggggcaattg
     2941 ttggtctagt tctggttatt ttaaccaaaa ccgggttgtt tggattcaga aaatatttta
     3001 caattaagag tgagcttcct ttcgcgccgt ttatcatttt gggtgtgctt ctaaatttgc
     3061 ttttccaagt caatgtcttt taaaaaatca caacaaggtt tttcgctgct tgagttgatg
     3121 gtggttatca cgattttttt gattatcacc gccgtggtga tggccgatat tccaattttg
     3181 gtcgcaaggg ggcgcttgac ttgaccgcgc aagaagtagc cggttgcata cgagcagccc
     3241 aagtttatgg cacggctgaa aaagtagttg gc
//
LOCUS       DAOSHV010000061         3060 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_30217_length_3060_cov_5.830616, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000061 DAOSHV010000000
VERSION     DAOSHV010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30217_length_3060_cov_5.830616"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..771
                     /locus_tag="PKZ29_02475"
     CDS             <1..771
                     /locus_tag="PKZ29_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37764.1"
                     /translation="KRAYEVVWQPLLKGKFHNYYRKVSMAWLWARIHTRGNSTNEKGE
                     EVLGYINGGFETIVARLAKLIKKNGGKIKLNTNIKDLTALEKEFEIMVDTRPAKEVEY
                     LGAIDVVFRSKQNLSPYYWHNINDTESPFVAVVQHTNLIEKKNYNNKDVYYLGSYLPQ
                     NHKFFGWSDKKITDNWFSYVKRIFPEFDPIMVDKVSVFRFKSAQHIVTTNYAQKIPPQ
                     KISAKIYQINFAQIYPEDRGLNFAVREAKKMVKVIFES"
     gene            complement(749..1639)
                     /locus_tag="PKZ29_02480"
     CDS             complement(749..1639)
                     /locus_tag="PKZ29_02480"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HOG37765.1"
                     /translation="MKTKIIGTGLTGLVGSRIVELLNDKFEFIDFSLDKGINILDQNS
                     LSQAFASHQDAVAVIHLAAFTDTNAAWEQRGDKSGLCYQLNVVGTENILDLAKKNNQY
                     FIYISTDFVFDGAKTTPYIESDTPHPIEWYGETKYLGEQAVLNSPCPSAITRITYPYR
                     ANFDLKPDIIHKVLTKLQAGETVKMFSDQICTYTFIDDIAVALGQFLDKKYTGIYHLV
                     GSSSHSPFDMAKLVAQTFGCDPNLVQASSLDEYIKSQPQDSRPWQKTLITSNQKAINL
                     GMKFKTLAEGLAKIRTQISP"
     gene            complement(1667..2224)
                     /gene="rfbC"
                     /locus_tag="PKZ29_02485"
     CDS             complement(1667..2224)
                     /gene="rfbC"
                     /locus_tag="PKZ29_02485"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013237240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HOG37766.1"
                     /translation="MPDFIKTKIDGLVIIKPSVFEDDRGFFLESYNQKVFADNGINAN
                     FVQDNHSRSTKGVLRGLHFQTPPFSQDKLIRCTRGEVFDVAVDIRPGSPTFGQYHSVT
                     LSEDNKIMFFIPQGFAHGFLVLSDIADFQYKCSNLYHKDSEGGLLWNDPKLNIDWPKV
                     DHLILSDKDKLWPTLKEFKTSLQTL"
     gene            complement(2224..>3060)
                     /gene="rfbB"
                     /locus_tag="PKZ29_02490"
     CDS             complement(2224..>3060)
                     /gene="rfbB"
                     /locus_tag="PKZ29_02490"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HOG37767.1"
                     /translation="ILDDKLVDQIFSQGIDTVVHFAAESHVDRSIDDPSLFVKTNVLG
                     TYVLLHAAAKYGVKRFHHVSTDEVFGSIALDSKSQFKETTPYDPSSPYSASKAASDHL
                     VRSFFKTFGLPVTISNCSNNYGPYQHPEKMVPRSITNLLSSDKIKVYGQGLNFRDWLH
                     VEDHCRAIELILLKGKIGETYCIGGLKKGTSNMDLVNLILKLMGKDETSLEFVADRPA
                     HDNYAVNWDKINKDLGWQPKYDLESGLKQTIDWYTQNESWWQETKKEAEAFYLKLNQS
                     KK"
BASE COUNT         1002 a          480 c          682 g          896 t
ORIGIN      
        1 aaaagagctt atgaagtagt gtggcagccg cttttaaaag gaaaatttca taattattac
       61 agaaaggtgt cgatggcgtg gctgtgggcc agaatccata cccgaggcaa ttcgactaac
      121 gaaaaagggg aagaagtgtt gggatatatc aacggtggat ttgaaacgat agtggcaaga
      181 ttggctaaat taatcaaaaa aaatggcgga aaaataaaat taaatactaa cattaaagat
      241 ctaacagctt tagaaaaaga atttgaaatt atggttgata ccagaccagc caaggaagtg
      301 gaatatttgg gggcaattga tgtggtattt agaagtaaac aaaatttaag tccgtattat
      361 tggcataata taaacgacac cgaatcccct tttgtggcgg tggtgcaaca taccaatttg
      421 attgagaaaa agaattataa caacaaagat gtctattatt tggggtctta tctgccgcaa
      481 aatcataaat tttttggttg gagtgataaa aaaataacag acaactggtt tagttatgtc
      541 aaaaggatat ttcctgaatt tgatccaata atggtggata aagtatcggt gtttaggttt
      601 aaatcggctc aacacatagt gacaactaat tatgctcaaa aaatcccgcc tcaaaaaatt
      661 tcggctaaaa tttatcagat aaattttgcc caaatttatc cagaggatag gggtctaaat
      721 tttgcagtca gggaagcaaa aaaaatggtt aaggtgatat ttgagtccta attttggcta
      781 aaccttctgc tagagtttta aatttcattc ctaaatttat ggctttttgg ttggaagtaa
      841 tcagagtttt ttgccaagga cgactgtctt ggggttgaga tttaatatat tcgtctaaac
      901 tggaagcttg aactaaatta ggatcacaac caaaagtttg ggcgacaagt ttggccatat
      961 caaaaggtga gtgagaagaa gaaccaacta aatgatagat gccggtatat tttttgtcta
     1021 aaaattgacc taaagcaacg gcaatatcat caataaaggt ataggtacaa atttggtcag
     1081 aaaacatttt gacagtttca ccggcttgga gtttggttaa aactttgtga ataatatcag
     1141 gcttgagatc aaaattagcc cgataggggt aggtgatgcg ggtgatagct gaagggcaag
     1201 gagagttgag gacggcttgt tcacccagat atttagtttc accataccac tcgataggat
     1261 gaggagtatc agattcgata tagggagtag ttttggcgcc gtcaaagaca aagtcggtag
     1321 aaatataaat aaaatattga ttgttttttt tggctaaatc tagaatattt tcggtgccga
     1381 caacattgag ctgatagcaa agacctgact tgtcccctct ttgctcccag gcggcattag
     1441 tatcagtaaa agcagctaaa tggataacgg cgacggcatc ttggtgggaa gcaaaggctt
     1501 gagataaact gttttggtct aaaatattaa tccccttatc aagagaaaaa tcaataaatt
     1561 caaatttatc atttaggagt tcaacaatcc gactgccaac caaaccggtt agaccagtac
     1621 ctattatttt agttttcaag ttagaaagtt tttaaagtta taaagtttat aaagtttgta
     1681 aagaagtttt gaattctttt agagttggcc aaagtttatc cttgtcggac aaaataagat
     1741 ggtcaacttt aggccagtca atattaagtt tggggtcatt ccaaagcaag ccaccttcag
     1801 agtctttgtg ataaagattg gagcatttat attggaaatc agctatatcg gataaaacta
     1861 aaaatccatg agcaaaacct tggggaataa aaaacataat tttgttgtct tcagacaagg
     1921 tgactgaatg atattggcca aaggtgggag aacctggacg aatatcaacg gcaacatcaa
     1981 aaacctctcc tctggtgcag cgaataagtt tgtcctgaga aaatggcggg gtttgaaaat
     2041 gaagcccgcg aagcacgcct ttagtagaac gggaatgatt gtcctgaaca aaattagcgt
     2101 tgataccgtt gtcggcaaaa actttttgat tgtagctttc gagaaaaaat cccctatcat
     2161 cttcaaaaac agaaggtttg ataatcacaa gtccatcaat tttggttttg ataaaatctg
     2221 gcattatttt ttggattggt ttaatttgag atagaatgct tcggcttctt ttttggtttc
     2281 ctgccaccaa gattcgtttt gggtatacca atcaatagtt tgtttgagtc cggactctaa
     2341 atcgtatttg ggctgccaac caaggtcttt gtttattttg tcccaattta cagcataatt
     2401 atcatgagcc ggtcggtcgg cgacaaattc tagagaggtt tcgtctttgc ccataagttt
     2461 gaggattaga ttgactaggt ccatattgga agtgcctttt ttaagaccgc caatgcagta
     2521 agtttctcca attttaccct taagaaggat taattcgatg gcccggcaat gatcttcaac
     2581 atgaagccag tctctaaaat taagaccctg accatagact ttgattttgt cgctggaaag
     2641 aagattggta atacttcggg gaaccatttt ttcggggtgt tgataaggac catagttgtt
     2701 ggaacagttg ctgatagtga ccgggagtcc gaaggttttg aaaaatgatc tgactaaatg
     2761 gtcagaagca gctttggaag cggaataggg gctggaggga tcgtaaggag tggtttcttt
     2821 gaattgagat ttagaatcga gagcaatact accaaaaact tcgtcggtgg aaacatgatg
     2881 aaatcttttg acgccgtatt tggcagcggc gtgaaggaga acataggtgc caaggacgtt
     2941 agttttgaca aataatgaag ggtcgtcaat tgaacgatcg acgtgagact cagcggcaaa
     3001 atgaaccaca gtatcaattc cctgggaaaa aatttgatca accaatttgt catcaagaat
//
LOCUS       DAOSHV010000062         2949 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_31859_length_2949_cov_5.146164, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000062 DAOSHV010000000
VERSION     DAOSHV010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31859_length_2949_cov_5.146164"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..867)
                     /locus_tag="PKZ29_02495"
     CDS             complement(<1..867)
                     /locus_tag="PKZ29_02495"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HOG37768.1"
                     /translation="MSSLNQLTLTQAVDGIKSKKFSASELVQDCFDRIKKLEPHLNAF
                     ISLDKTTALAKAQKIDFDKPLAGIPIAVKDNFCIKGIKTTASSNLLKNYTAQYTATVV
                     SKLEDAGAIIVGKNNMDAWAHGSSTETSDFGPSKNPWNFDYLPGGSSGGSAAAIAADE
                     VIAAIGTETAGSIRQPSAWCGVVGLKPTYGRVSRYGIIAMASSMDCPGPITKTVDDAA
                     LILSLISGHDIHDATSLPQKPLNLDLSKKDLKGLKIGLPKAYLPDNIHPAVKDLILQA
                     AQTLKSLGAQIID"
     gene            complement(870..1169)
                     /gene="gatC"
                     /locus_tag="PKZ29_02500"
     CDS             complement(870..1169)
                     /gene="gatC"
                     /locus_tag="PKZ29_02500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HOG37769.1"
                     /translation="MSSTKISPEQLKHIANLARLEIKADKQNLLIKQLSQTAEYIDIL
                     SQVDTKNIAPTYQVNHLKNVCRKDEVGQSLSQSEALSQAPKTHNGYFQTQATIKK"
     gene            complement(1183..2217)
                     /locus_tag="PKZ29_02505"
     CDS             complement(1183..2217)
                     /locus_tag="PKZ29_02505"
                     /inference="COORDINATES: protein motif:HMM:NF014445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HOG37770.1"
                     /translation="MTRIFTNMTIKLLTHPKDKPKYNSVVDHPVQTWEWGEFQQSQGH
                     KIFRFGVFDQKNKIVSAYTISFHQIPKTKFSIGTILRGPVVDDDMLQNIKQVALEQNA
                     IFVKLEPNIPKSQASMQFSNLVVSPKVAFYPHTFIIDTTKTEDELMASLHPKTRYNIK
                     VANRHGVEIKEATNDKGFEIYLKLLLSTTKRQGFYLHTAKYHRDLWQLLQHTDIPHIM
                     LASYQGEVLSAFMIFKLKDRLFYPYGASLDTYRQVMAPTLLMWETIKLAKKLKCLTFD
                     MWGCLGPNAQEGENGFGFHRFKQGFGGQMLEYVGTYDFVINPNLYKIYNLVDKLRWTF
                     LRLKAKVFKK"
     gene            complement(2198..>2949)
                     /locus_tag="PKZ29_02510"
     CDS             complement(2198..>2949)
                     /locus_tag="PKZ29_02510"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOG37771.1"
                     /translation="GGTRFYERAEIKDILAMIRFADNRRDKISLDRLNKAFGKRRLSV
                     FMTHLDNLDISDLNSLQVLESLVTASGYLDRYDPKDENDQKKIENIKELKSVAATFPR
                     LSDFLENVALVQQEYSLQEKNKKKENRDGVRLMTLHGAKGLEFEVVFLVGFEEGILPH
                     SRCLIDEAEIEEERRLCYVGITRAKDYLYISYASQRLYFGKSSLNEPSRFLTDIPNNL
                     IELDEPQEIVRDYRRHRESSDDDLIYDPDIY"
BASE COUNT          838 a          526 c          733 g          852 t
ORIGIN      
        1 atcaatgatt tgagctccta aactttttag ggtttgggcg gcctgaagaa taaggtcttt
       61 aaccgccggg tgaatattat caggaagata agcttttggc agaccaattt taagaccctt
      121 aagatctttt tttgaaagat ctagattgag gggtttttga ggcagactgg tggcatcgtg
      181 gatatcgtga ccagaaataa gggaaagaat tagggcggca tcatcaacag ttttggtaat
      241 agggccgggg caatccatag aagaggccat ggcgatgatg ccataacgac tgacgcggcc
      301 ataagtcggt ttgagtccga ctactccaca ccaagctgaa ggttggcgaa tagagccggc
      361 agtttcggtg ccgatggcag caataacttc gtcggcggcg atagcggcag ctgagccgcc
      421 ggaggatcca cccggaagat aatcaaaatt ccagggattt ttgctgggac caaaatcaga
      481 ggtttcggta gaggaaccat gggcccaagc atccatatta tttttgccga cgatgatggc
      541 accggcgtct tcgagtttgg aaacaacggt ggcagtatat tgagcagtat agtttttgag
      601 aagattgctg gaggcagtgg ttttgatacc cttgatgcaa aaattatctt tgacggcaat
      661 aggaatgcca gctaagggtt tatcaaaatc aattttttga gctttggcta aagcagtggt
      721 tttgtcgagg ctgataaaag cgttaagatg gggttctaat tttttgatac gatcaaaaca
      781 gtcttggacc agctcagagg cagaaaattt tttggattta atcccgtcaa cggcttgagt
      841 tagggttaat tgatttagcg atgacataat tattttttga tagtggcttg ggtttggaaa
      901 tagccgttgt gggttttggg ggcttgagac aaggcttctg actgagacaa agactgacct
      961 acttcatctt tgcggcaaac atttttaaga tgattaactt gataggtagg agcgatgttc
     1021 ttggtgtcaa cttgggacaa aatatcaata tattcggcag tttgggataa ttgtttgata
     1081 aggagattct gcttatcggc tttaatttcc agccgggcaa gattggcgat gtgcttaagc
     1141 tgctcgggac taattttggt tgaagacata gtctatttta tcttattttt taaaaacttt
     1201 ggctttgaga cgaagaaaag tccagcgtaa tttgtcgaca agattataaa tcttatagag
     1261 attggggttg ataacaaaat cgtaagtgcc gacgtattcc aacatctggc caccgaagcc
     1321 ctgcttgaaa cgatgaaaac caaagccatt ttccccttct tgggcgttgg gtccaagaca
     1381 gccccacata tcaaaggtga ggcatttaag ttttttggca agtttaatag tctcccacat
     1441 aagtaaggtg ggggccataa cctgacgata ggtgtctaag gaagcgccgt aaggataaaa
     1501 taagcggtct tttaatttaa aaatcataaa agcagacaaa acttcgcctt gatatgaagc
     1561 aagcatgata tgaggaatat cggtgtgttg gagcagctgc caaagatcgc ggtgatattt
     1621 ggcggtgtga agataaaacc cctgacgttt ggtggtgcta aggaggagtt tgagataaat
     1681 ctcaaaaccc ttgtcgttgg tggcttcttt aatctcaacc ccgtggcggt tggcaacctt
     1741 gatattgtat ctggttttgg gatgaaggga ggccatgagt tcatcttcgg ttttggtggt
     1801 atcaatgata aaagtatgag gataaaaggc aactttgggg gaaacgacca aattggagaa
     1861 ttgcattgaa gcttgagatt tgggaatatt cggttccagt ttgacaaaaa tggcgttttg
     1921 ctccaaggca acttgtttaa tattttgaag catgtcatca tcgacgacag ggccacgcaa
     1981 aatagtgccg atggaaaact tggttttggg gatttggtga aaactgatgg tgtaagccga
     2041 gacaattttg tttttttgat caaaaacgcc gaaacggaaa attttgtgcc cttggctttg
     2101 ttgaaattcg ccccactccc aggtttgaac cgggtggtcg acaactgaat tatattttgg
     2161 tttgtctttg ggatgagtaa gaagtttgat cgtcatatta gtaaatatcc gggtcataaa
     2221 taagatcatc atcagaggat tcgcggtggc ggcgataatc cctgacaatt tcttggggtt
     2281 catctaattc tatcaaatta tttggaatat cggttaaaaa ccggctgggt tcattgaggc
     2341 tacttttgcc aaaataaagc cgctggctgg cataagagat ataaaggtaa tctttggccc
     2401 gggtgatgcc tacataacaa agacggcgtt cttcttcgat ttcggcttcg tcaattaaac
     2461 accgtgagtg aggcaaaatc ccctcttcaa aacccaccaa aaaaaccact tcaaattcca
     2521 aacctttggc gccatggaga gtcataagtc tgacgccatc gcgattttct ttctttttgt
     2581 ttttttcctg aagagaatat tcctgttgaa ccagggcgac attttccaaa aaatcgctaa
     2641 gacggggaaa ggtggcggca acagatttta attccttgat gttttcaatt ttcttttggt
     2701 cattttcgtc tttggggtcg taacgatcaa gatagccgga agcagtaact aaagattcta
     2761 agacctgaag agaatttaga tcagagatgt caagattatc aagatgagtc ataaatactg
     2821 acaggcggcg tttaccaaaa gccttgttaa gccgatcaag agaaattttg tcacgacggt
     2881 tatcggcaaa acgaatcata gccaaaatat ctttaatctc ggctctttca taaaaccggg
     2941 tgccgccga
//
LOCUS       DAOSHV010000063         2925 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_32242_length_2925_cov_5.940070, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000063 DAOSHV010000000
VERSION     DAOSHV010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32242_length_2925_cov_5.940070"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..862)
                     /locus_tag="PKZ29_02515"
     CDS             complement(<1..862)
                     /locus_tag="PKZ29_02515"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOG37772.1"
                     /translation="MKAVIICGGVGSKMWPMSRSESPKHFLPLLGGKSLFQLNWEALR
                     LKFSPEEIFLQTNEAQAKIAVSQVSEIKEENIFVEPEMRNQGPATGFAAAQLIKRGFG
                     EEMFILVQADVLREPAEKFIEMIEEMEKHSSGGKYMTGGLVPESIVRGVDYLVKGNLV
                     SETNGVKIFEVADYIDRTEEEKIKQYLGTDKLVLHANHTAMTPNGLLKMYQTYKPEWY
                     EPLVAISEGGEVISEYAKMPKGAIEEVTKLVYQKNEALVVELPFDWVDFGTWESVANY
                     MKEKGIYDQSD"
     gene            complement(1177..2184)
                     /locus_tag="PKZ29_02520"
     CDS             complement(1177..2184)
                     /locus_tag="PKZ29_02520"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOG37773.1"
                     /translation="MKQQETKVAFFGVKAWERDIIEREISSLDTFGVGIFEQEVQEAI
                     ELAKNYEVISPFIYSKLDKKVLVKLPKLKLVATRSTGFDHIDSDYCKSKKIKIATVPE
                     YGSDTVAEYAFALLLAIAKKVVVANESVEDGNFSPEGLTGVDLKGKTLGVIGVGKIGQ
                     NVVKIARGFGMKVLGVDAYKDPKMVKKAGFEYADLETCLKKADFVTLHVPANPKTFHL
                     INKTNIKLMKSGSYIINTARGAVVEIEALVWGLNHGILAGVGLDVTEQEMRVDDTETV
                     MDKSLTKDSLQDILSFHLLRDRDDVVFTPHNAFNTKEAIERIVKTTVKNINEFIKLSS
                     S"
     gene            complement(2295..>2925)
                     /locus_tag="PKZ29_02525"
     CDS             complement(2295..>2925)
                     /locus_tag="PKZ29_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37774.1"
                     /translation="ETKIDTDQEMLKKAFNAAESGIEHYFGTGSTKYTGDDIQGSANL
                     SVTSFGDSQKLSFNNVTLPGGTEYFWLVDRLTDGTLGTSYYSTNNPSISICDRDGKTG
                     KYKISYFYLSGATYTRTNQTLDTDSTGCIAGNFNLSAGRSLLLTVTPIGFASKIQLGG
                     STNFPIQGEEISSTGMVSGVNNTVTILNRYEIPTFMLEAITSGGKVTNK"
BASE COUNT          771 a          776 c          417 g          961 t
ORIGIN      
        1 catcactctg atcataaatt cccttttctt tcatataatt agccaccgac tcccaggtgc
       61 caaaatccac ccagtcaaac ggcagttcca ccaccaaagc ttcatttttt tgatatacca
      121 gtttggtcac ttcctcaatt gccccttttg gcattttggc atattccgat atcacctctc
      181 ctccctcaga aattgccacc aacggctcat accattccgg tttataagtc tgatacattt
      241 ttagcaatcc attcggcgtc atagctgtat ggttagcatg aagcaccagc ttatctgttc
      301 ccaaatattg tttaattttt tcctcttccg tccggtcaat ataatccgcc acctcaaaaa
      361 ttttcactcc attagtttca cttaccaaat taccttttac caaataatcc acccctcgga
      421 caatcgactc cggcaccaac cctcccgtca tatactttcc cccactgcta tgtttttcca
      481 tctcttcaat catttctata aatttttctg ccggctctct taacacatcc gcctgcacca
      541 aaataaacat ttcttctcca aaaccccgct tgattaactg agccgccgca aatccggttg
      601 ccggcccctg atttcgcatc tccggctcga caaatatatt ttcttcctta atttccgata
      661 cttgcgacac tgcaattttg gcttgcgcct catttgtctg caaaaatatt tcttccggac
      721 taaattttaa cctcaaggct tcccaattca actgaaacag cgactttcct cccaacaacg
      781 gcaaaaaatg ttttgggctt tcacttcggc tcatcggcca catttttgaa cctaccccac
      841 cgcaaataat gactgctttc atctttaaat ttaacttcta acttaatcca ttatacctta
      901 acctctattc caactcctca aaatccaacc ctaacaacga taatcaatca aaataagata
      961 atatttctta atttaattcc cgcaacccga aatcatcatt ttggaacgta tttgagcctg
     1021 cgttgcagac ctcattttcc agtggaaaaa tgatgatgag ggttgatccc aaaaaacttt
     1081 ataaacttaa ttcaatccct ctaaatctcc ctttgacaag ggagattttc tcctcccctc
     1141 ttgttaaaga ggggttgggg gagattgtgt tttactttat gaacttgata acttgataaa
     1201 ctcattaata tttttgaccg tcgttttcac aattctttca atcgcctcct tggtattaaa
     1261 cgcattgtgc ggcgtaaata ccacatcatc ccgatctctt agcaaatgaa agctcaaaat
     1321 atcctgcaga ctgtccttgg ttagactttt gtccatcacc gtctcagtat catccactct
     1381 catttcctgc tccgttacat ccagccccac ccccgccaaa atcccatgat ttaatcccca
     1441 taccaacgcc tctatttcta ccaccgctcc cctagcggtg ttgatgatat agctccccga
     1501 tttcattaac tttatattgg tcttatttat taaatgaaat gtttttgggt tagccggcac
     1561 gtgcagcgtc acaaaatccg ccttttttaa acaagtttct aaatccgcat attcaaatcc
     1621 ggcttttttt accatttttg gatctttgta ggcatccact cccaatactt tcattccaaa
     1681 accccgggcg attttgacta cattttgccc gattttcccc acgccgatta cccccaaagt
     1741 ttttcccttc aaatccactc ccgtcaaccc ttccggacta aaattaccat cctctaccga
     1801 ctcgttggcc actaccacct ttttggctat tgccaacagc agagcaaagg catattctgc
     1861 taccgtatca gaaccatatt ccggcacagt tgcaatcttg attttttttg acttgcaata
     1921 atcgctgtca atatgatcaa atccggtcga tctggtggcc actaatttta gttttggcag
     1981 ttttaccaaa acttttttgt ccaatttgga ataaataaat ggtgaaatta cctcataatt
     2041 ctttgccaac tcaattgcct cctgaacttc ttgttcaaaa atacccactc caaatgtatc
     2101 caaactactt atttcccgct caattatgtc tctttcccac gccttaaccc caaaaaatgc
     2161 cacttttgtc tcttgttgtt tcatatttcc attataacaa tatattttca cccacacccc
     2221 tcagattttc ttttttatct tgcacatccc ttctacttga caaactttac gaactttcaa
     2281 cttgataaac taccctattt atttgtcacc tttccacctg aagttatcgc ttccagcatg
     2341 aaggtcggaa tctcataacg attaaggata gtgacggtat tattaactcc tgagaccatt
     2401 ccggtagaac taatctcttc tccctggata ggaaagttag ttgaacctcc cagttggatt
     2461 tttgaggcaa aaccaattgg ggttacggtc agcagtagcg atctgccagc tgacaggtta
     2521 aagtttccag caatacaacc ggttgaatcg gtatccaaag tttgattagt tctggtatag
     2581 gtggctcctg acagatagaa gtaactgatc ttgtattttc ctgtcttgcc atctcgatca
     2641 cagatagaaa ttgaagggtt gttggtgctg taataactag ttcccagagt tccgtctgtc
     2701 agtctgtcaa ccaaccagaa gtattctgtc cctcctggta aagttacatt gttaaatgat
     2761 aatttctgac tgtcaccaaa gcttgtaaca cttagattag ctgatccttg aatatcatca
     2821 ccggtgtatt tggtgctgcc ggtaccaaag taatgttcaa tccctgattc agctgcatta
     2881 aaagcttttt ttaacatctc ttgatcggta tcaattttag tttcc
//
LOCUS       DAOSHV010000064         2840 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_33666_length_2840_cov_5.355117, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000064 DAOSHV010000000
VERSION     DAOSHV010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33666_length_2840_cov_5.355117"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..853)
                     /locus_tag="PKZ29_02530"
     CDS             complement(<1..853)
                     /locus_tag="PKZ29_02530"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOG37775.1"
                     /translation="MKPAPNSRSLILIFSVGFFIVLATYFISLIARGYRFSLKDGFSL
                     EPTGMISAVSRPKGASVYLDDKLITATDDTISLSPGVYQLKITKDGFLPWQKTVTVKP
                     ETVYQADIQLFSTVPNLKPVTLTGAINPAVNLDNTKIVYAVASASAAKDNGLYLIESA
                     GLPLSLIRSDSRQIAVNTATIDWSDFSFTFSPNSRQILATNSLERINYLLPLDAPISP
                     QTLVDITHNLDTIHLDWQTQTTAILTAKLQSLPKDIQPFIATGSAQNLQFSSDDNKIL
                     YLAQTDGN"
     gene            934..1935
                     /locus_tag="PKZ29_02535"
     CDS             934..1935
                     /locus_tag="PKZ29_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004094482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOG37776.1"
                     /translation="MKFFKKLAIDLGTANSLVWEAGKGVVLNEPTIVAVGIEDRKVLA
                     VGNEAKKMLGKTPEYIEVIRPLEEGVIADYEVTEAMLRYFLQQVMGNKWFIGPEVMVC
                     VPAGVTQVEQRAVLDAALSAGARKAYLIDEPLAAAIGAKIPVSEAFGNMIVDVGGGAA
                     EAAVIALGGVVTHKSVKVGGGKLDMAIVDFLKKNFNLVVGEQTAEAIKIKLGSAIKPK
                     KVESIEVNGRDTVYGLPKNMMMDNEMVYEAIKPMLEMIMMSIRNTLEITPPELVADIV
                     DRGIVLSGGTVQLKNFNTLVTREIGVSAHVALEPQLCVIKGTGMAVENLEVYRRAIK"
     gene            2274..>2840
                     /locus_tag="PKZ29_02540"
     CDS             2274..>2840
                     /locus_tag="PKZ29_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37777.1"
                     /translation="MSKKKVKKQAQKPKKAKKGVKFDDTYSLKYWKMFDIPLFGGDKS
                     RVLKELENQIRQGRKKYWVATVNPEFVMAGEKDRWFKRILQTKTSLNVVDGIGLIWAF
                     ELIKSTSFKVDKVRSKFLKFMTRWWWGIKTGVDVLRGKYKERVASGADLVVDLAKMAK
                     KYNKKIFLLGGWGDRAVKTGEYLIKKTGL"
BASE COUNT          881 a          334 c          884 g          741 t
ORIGIN      
        1 tattgccgtc agtttgggct aaataaagaa ttttattgtc atcgctggag aattggagat
       61 tttgagcaga accggtggca ataaagggtt gaatgtcttt gggaagagac tggagcttgg
      121 cagtaagaat ggcggtggtt tgggtctgcc agtcaagatg aatagtatcg aggttatggg
      181 taatatcgac gagagtttgg ggagagatag gagcgtcaag cggcaaaagg tagtttattc
      241 tctcaagaga attggtggct agaatttggc gagagtttgg ggaaaaagtg aaactgaagt
      301 cagaccagtc gatggtggcg gtattgacgg caatttggcg ggaatcgctg cggatgagag
      361 aaagtggcag accagcggat tcaattagat aaagaccatt gtctttggcg gcggaggcag
      421 aggcaacggc ataaactatt ttagtgttat caaggttgac ggcggggttg atggcgccgg
      481 tgagagtgac gggtttgaga ttgggaacgg tgctaaaaag ttgaatatca gcctgataga
      541 cagtttcagg tttgacagta acggtttttt gccagggaag aaagccgtct ttggtaattt
      601 ttagctggta aacaccaggg gaaagactga tggtgtcgtc ggtggcggta atgagtttat
      661 catcaagata gacggaggcg cctttgggtc gggacacggc ggaaatcatg ccggtgggtt
      721 cgagactgaa gccgtctttg agagaaaaac gataaccccg ggcaataaga gagataaagt
      781 aagtagctag aacaataaaa aagccaacag aaaaaattag aatcaggctg cggctgttag
      841 gagcaggttt catatagcct tattataact taatagagga gtggtgagtg atttaggata
      901 tatgaattgg gaataaaacc gctaaaatta agtatgaaat tttttaagaa gctggcgatt
      961 gatttgggga cggccaatag tttggtatgg gaggcaggca aaggggtggt gttgaatgaa
     1021 ccgacaatag tggcggtggg aattgaggat agaaaggtgc tggcggtggg gaatgaggcc
     1081 aaaaaaatgt tggggaaaac accggagtat attgaggtta tccgaccgtt ggaggaaggg
     1141 gtgattgctg attatgaagt gacggaggcg atgttgaggt attttttgca gcaggtgatg
     1201 gggaataaat ggtttatcgg accggaggtg atggtgtgtg tgccggcggg agtgactcag
     1261 gtagagcaaa gggcggtgtt ggacgcggct ttgtcggcgg gggcgcgaaa ggcttatttg
     1321 attgacgagc cgttggcggc ggcaattggg gcaaaaatac cggtatctga ggcatttggc
     1381 aatatgatag ttgatgtcgg cggcggggcg gcggaagcag ccgtaattgc tttgggtggg
     1441 gtagtgaccc ataagagtgt taaagtgggc ggcggtaagc tggatatggc gattgtggat
     1501 tttttgaaaa agaattttaa cctggtggtg ggggaacaga cagccgaggc aatcaaaata
     1561 aaattggggt cggcaattaa acccaaaaaa gtagaatcga tagaggttaa cggtcgggat
     1621 acagtttatg gtctgccaaa aaatatgatg atggataacg aaatggtgta tgaggcgatt
     1681 aagccgatgc tggaaatgat tatgatgtca attagaaata ctttggaaat aactccgccg
     1741 gagttggtgg cggatattgt ggaccgaggg atagtgctta gcggggggac ggttcagttg
     1801 aagaatttta acaccctggt gacaagagaa attggggtgt cggcgcacgt ggcattggag
     1861 ccacaattgt gtgttataaa agggacgggg atggcggtgg aaaatttgga agtctaccgt
     1921 cgggcgatta agtgatatag ttcatatttt taacctataa aaatgttaat aactaatttt
     1981 ttgggattta ttttgggaaa aatattggca gtataagtgg agatttaaga aaattaatat
     2041 ttttgagata atataatata tttgactatt ttacggtgat atttttgagt aattaatttg
     2101 tgcaaaatag taaaaatcgg ggaaaataga ttttgagaaa aacagcttgt aatatttaag
     2161 gacaaaaatg ggtgatatag ttaagttaat atagttattt ttttaaaata taggatagtt
     2221 tttgagatat ttttgcccca aaatttgcgg gggaaaaatg ctaaaattga ccagtgagta
     2281 aaaaaaaggt aaaaaaacag gctcaaaagc caaaaaaggc aaaaaaaggg gtgaagtttg
     2341 atgacactta ttccctaaag tattggaaaa tgtttgatat tcctcttttt ggcggagata
     2401 agagtcgagt gctaaaagag ttggaaaatc aaattcggca aggaaggaaa aaatattggg
     2461 tggcaacggt aaatccggag tttgtgatgg cgggggagaa agataggtgg tttaaaagaa
     2521 ttttgcaaac caaaacgagt ctaaatgtgg tagatggaat tgggttgatt tgggcgtttg
     2581 agcttatcaa gtcaacaagc tttaaagtcg ataaagtaag aagcaaattt ttgaagttta
     2641 tgacaaggtg gtggtggggt attaaaaccg gggtggatgt tttgagggga aaatataagg
     2701 agagggtggc gtcgggggcg gacttggtgg tggatttggc caagatggca aaaaaatata
     2761 ataaaaagat atttttgctg gggggatggg gtgatcgagc tgtaaaaaca ggggaatatt
     2821 taataaaaaa gacaggtttg
//
LOCUS       DAOSHV010000065         2644 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_37362_length_2644_cov_5.606412, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000065 DAOSHV010000000
VERSION     DAOSHV010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37362_length_2644_cov_5.606412"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="PKZ29_02545"
     CDS             <1..233
                     /locus_tag="PKZ29_02545"
                     /inference="COORDINATES: protein motif:HMM:NF040134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37778.1"
                     /translation="MYVLVPGLVMAELGMVMSFWPVGIFKGSIYLVAAVYILAGLIQL
                     DLRERLFKRSWVIFSWITVAIVLGMILTTSWR"
     gene            complement(230..1174)
                     /gene="pta"
                     /locus_tag="PKZ29_02550"
     CDS             complement(230..1174)
                     /gene="pta"
                     /locus_tag="PKZ29_02550"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009611063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HOG37779.1"
                     /translation="MNPIIQKIYTQAQSNLQKIVLADAHDSRIQAAAKIATDQKIADI
                     ILLTEEYLHHNLETQNNLAQNLFQLRSDKGLTLNQAQELITQPIYFGTMMVKTGLADG
                     LVSGAASTTHDTFKPGLQIIKTAPNQNIISSFFIMEFPHPDIGYQGIMVFADCGINIS
                     PNTPTLAQITQQSIDSFVKLIGPSPRVALLSYSTNGSSSGPSVDTVHQATQIIKSQYP
                     DLIIEGEIQADAALSPQVSLQKDPHSQLGGQADILIFPDLNSGNIGYKLVERLAGAKA
                     YGPISQGIAKPINDLSRGCTVKDIVTTIAITAVQAQTS"
     gene            1233..>2644
                     /locus_tag="PKZ29_02555"
     CDS             1233..>2644
                     /locus_tag="PKZ29_02555"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG37780.1"
                     /translation="MKDKTKKTLKYYWGAMKNHKTSGLILTAAVILGSVLDTIVPILS
                     KNFFNILFDGGVREEIVKSLMGVLILMAVIKFLRWLLWRVASFSINFFESRTIVDLSR
                     QGFDYLQKHSFSYFSNNFPGSTVKKHKSFINTFEFLADQVFFEIIPGIVMVMVMIFVL
                     GRVNIWLGLGMLVWMAIFLLINWWFTKYKLKYDIQRSEAETATSGLLSDTVSNNFNIK
                     LFNGYIYESETYGNLSENLHKIRKFTWDMGAVFYGVQSFLMLALEILIYYLAVRWWQR
                     DLLTVGDFVLIQSYLFSAVMLVWDFGRIMMRIYERLAEAEEMTEILSLPHGVVDKKGS
                     KKLKVKSGEIKFEDVSFKYEDGEKVIKDFNLEVKPGQTVALVGLSGSGKSTLAKLLLR
                     LYDVDSGRILVDGQNIAGVSQESLRHSISLVPQDPILFHRSLMENIRYGRFEATDEEV
                     IAAAKLAYCDGFISKLKDGYG"
BASE COUNT          727 a          336 c          753 g          828 t
ORIGIN      
        1 ggatgtatgt gttggtgccg ggattggtga tggcagaatt gggaatggtg atgtcgtttt
       61 ggccggtggg gatatttaag gggtcaattt atttggtggc ggcggtttat attttggcag
      121 gtctaattca acttgacttg agagaaaggt tatttaaaag aagttgggtc atattttcct
      181 ggattacggt agcaattgtg ttgggaatga tactgacgac cagttggcgc taggatgttt
      241 gggcttggac agcggtaatg gcgatagtgg tgacaatgtc tttaacagtg cagccgcggg
      301 aaaggtcgtt gatgggtttg gctatgcctt gggagatggg gccgtaggct ttggcgccgg
      361 caagacgctc gactaattta tagccgatgt tgccgctatt aagatcggga aaaatgagga
      421 tatcagcttg accaccaagt tggctatggg gatctttttg gagagagact tgaggggaga
      481 gggcggcgtc agcctggatt tcaccctcaa taattaagtc gggatattga gatttgataa
      541 tttgagtggc ttgatggacg gtgtcaacac tgggtccgct gctgctgccg ttggtagaat
      601 aggagagaag agcaacccgg ggagagggac cgataagttt gacaaaactg tcgattgatt
      661 gttgggtgat ttgggcaaga gttggtgtgt ttggactaat gtttattcca caatcagcaa
      721 aaaccataat cccttggtag ccgatgtcgg ggtggggaaa ttccataata aaaaaactgg
      781 agataatatt ttggttaggg gcggttttga taatttgaag tccgggtttg aaagtgtcgt
      841 gggtggtgct ggcagcgccg gagactaaac cgtcagccag accggttttc accatcatgg
      901 tgccaaaata aataggttgg gtaatgagtt cttgggcttg gtttaaggtt aaacctttgt
      961 cagaacggag ttgaaacaga ttttgggcta gattattttg agtttcaagg ttatgatgga
     1021 gatattcttc ggttaacaaa ataatatcgg cgattttttg gtcggtggca attttggcag
     1081 cggcctgaat tctggagtca tgggcgtcgg ctaaaacaat tttttggaga ttcgattggg
     1141 cttgggtgta aattttttgg ataatcggat tcataaaagt aattatatat taaatatacg
     1201 tcctaaaata gggtataatc aaaaatacaa aaatgaaaga taagaccaaa aagaccctta
     1261 aatattattg gggggcgatg aaaaaccata aaacgtcggg gttgattttg acagcggcgg
     1321 taattttggg gtcagtgttg gatacaattg tgccgatttt gtcgaaaaac ttttttaaca
     1381 ttctgtttga tggaggagtt agagaagaga ttgtcaaaag tctgatggga gtgttgatat
     1441 taatggcagt aatcaaattt ttgcggtggt tgttgtggcg ggtggcttcg ttttcgatca
     1501 acttttttga atcaagaact attgttgacc tgagtcggca agggtttgat tatttacaaa
     1561 aacattcatt ttcttatttt tctaataatt ttccgggatc aacagttaaa aaacataaga
     1621 gttttataaa cacgtttgag tttttggctg accaagtatt ttttgaaatt atcccgggga
     1681 tagtaatggt aatggtgatg atttttgtgt taggtcgggt aaatatatgg ttgggtttgg
     1741 gaatgctggt atggatggca atatttttgc taataaattg gtggtttacc aaatataagc
     1801 tgaaatatga tattcagaga agtgaggcgg aaacagctac cagtggattg ctgtctgaca
     1861 ctgtcagcaa taattttaat attaaactat ttaacggcta tatttatgag agtgagactt
     1921 atggcaactt gtcagaaaat ctccataaga tacgtaaatt tacttgggat atgggagcag
     1981 ttttttatgg ggtgcagtct tttttgatgt tggctttgga aattttaatt tattacttgg
     2041 ctgtcaggtg gtggcagaga gatttattga cggtggggga ttttgtgtta attcagtcat
     2101 acttattttc ggcggtgatg ctggtgtggg attttggtcg gataatgatg cggatttatg
     2161 agcggttggc ggaagcagaa gaaatgacgg agattttgag tctgccgcat ggagtggtgg
     2221 ataaaaaagg atccaagaaa ctaaaggtaa aatcgggaga aattaaattt gaagatgtta
     2281 gttttaaata tgaagatgga gaaaaggtga ttaaagattt taatttggag gtaaagccgg
     2341 gacaaactgt ggctttggtg ggattatcag gatcgggtaa gtcaaccttg gcaaagctgc
     2401 tgctgagact ttatgatgtt gacagtgggc gaattttggt cgacggtcag aatattgccg
     2461 gggtgagtca ggaaagtttg agacatagta ttagtttagt gccgcaggat ccgatattat
     2521 ttcatcggag cttaatggaa aacattcgtt acggtcgatt tgaggcgacc gatgaggaag
     2581 taatagcagc agccaaactg gcttattgtg atggatttat ttctaaatta aaggatggtt
     2641 atgg
//
LOCUS       DAOSHV010000066         2448 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_41899_length_2448_cov_4.875888, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000066 DAOSHV010000000
VERSION     DAOSHV010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41899_length_2448_cov_4.875888"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="PKZ29_02560"
     CDS             <1..211
                     /locus_tag="PKZ29_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37781.1"
                     /translation="ELAEGKYMAAYQPNEIEIMGFYSQCALEMENCATPESHRLKEIE
                     VKPGVTVEKVDICDYYYQPENKPKW"
     gene            221..724
                     /locus_tag="PKZ29_02565"
     CDS             221..724
                     /locus_tag="PKZ29_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37782.1"
                     /translation="MADQKILKGVMAGLGELGAETLKKTGEEGQKMLETVISGKELLG
                     LEKTMDNQELEFKKQEEERKKQEEVAKLKQEMGYGRKVETEMTELRKQKEKEEEEKEE
                     YFEKVKQQQEREKQQQAEEYNDLMAVSNNPAKQKKSRGSALVTKKKHQPDMSQMSQTT
                     EFKGKID"
     gene            726..2102
                     /gene="cysS"
                     /locus_tag="PKZ29_02570"
     CDS             726..2102
                     /gene="cysS"
                     /locus_tag="PKZ29_02570"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOG37783.1"
                     /translation="MTELKFFNTLSRTKETFEPIETGKVGIYSCGPTVYSSPHIGNMY
                     AYVCWDVLVRVLQYLGYEVKNVINITDVGHLVSDGDSGEDKMEKGSKKEGLSVWEIAK
                     KYEDEFLTNLDELNIERPWKLPRATDHIAEQINLIQKIEANGYTYKTSDGIYFDTSKM
                     VDYGKMANLNLEKIKEGARVEVNEEKKHPTDFALWKFSPKDVKRQMEWESPWGVGFPG
                     WHIECTAMSTKYLGEKFDIHTGGEDHIPVHHTNEMAQAEGAFGHDLVRYWIHNAFITF
                     KGNKISKSSGGLWTIADLKKMGFEPLAFRYMVLSSHYRKGMEFSIESLKSAQTALLKL
                     RKYRVEDGKDYEVNQAFRQEFISKISDDLAMPEALAVVWKLMKSDLIVEEKWATLLDM
                     DKVLGLRLGETVFEEKIPEEIAQLAQQRQQARENKNWAESDRLRELIKSQGYLVEDLA
                     NSCKIRKI"
     gene            2148..2438
                     /gene="rpsF"
                     /locus_tag="PKZ29_02575"
     CDS             2148..2438
                     /gene="rpsF"
                     /locus_tag="PKZ29_02575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOG37784.1"
                     /translation="MKGNKYRYQLVIILNPKTENKDKTWEKVNGWLTKNKIESTQEHL
                     GLKELVYEIGKNSKGDFWTMDLTSESPIKQKDLNLLLNREPNIIRYLILKKE"
BASE COUNT          916 a          311 c          636 g          585 t
ORIGIN      
        1 ggaattagct gagggaaaat atatggcggc ttatcaacca aatgaaattg aaataatggg
       61 attttatagc cagtgcgccc tagagatgga aaattgcgcc acaccggagt ctcataggct
      121 aaaggaaata gaggtcaaac ccggggtgac ggtagaaaag gtggatatat gtgattatta
      181 ttatcaaccg gaaaataagc caaaatggta aaatgaattt atggcggatc aaaagatttt
      241 gaaaggagtg atggcaggat tgggtgagtt gggggcagag acactaaaaa agacgggtga
      301 ggaaggacaa aagatgttgg agacagtaat cagcggcaag gagttgttgg gattggaaaa
      361 gacgatggat aatcaggagt tggaatttaa aaaacaggaa gaggagagaa aaaagcagga
      421 ggaggtggca aaattgaaac aagaaatggg ttatggtaga aaagtagaga cggaaatgac
      481 ggaattaaga aaacaaaaag aaaaggaaga agaggaaaaa gaggaatatt ttgaaaaagt
      541 taaacaacaa caggaaagag aaaagcagca gcaggcggaa gaatataatg acttaatggc
      601 agtttcaaac aaccctgcca aacaaaagaa aagccgggga agcgctttgg tgacaaaaaa
      661 gaagcaccaa cctgatatga gtcaaatgtc gcaaacaact gagtttaaag gtaaaattga
      721 ctaagatgac ggagttaaaa ttttttaata ccctaagccg aactaaagaa acatttgaac
      781 cgattgagac aggaaaggtg gggatttaca gctgcgggcc gacggtctat tccagccctc
      841 atattggcaa tatgtatgct tatgtctgct gggatgtgtt ggtaagagtg ctgcaatatt
      901 tgggatatga agtcaaaaat gtgattaata ttactgacgt gggtcatttg gtgtctgacg
      961 gcgacagcgg cgaggataaa atggaaaaag gttcaaagaa agaaggcttg agtgtgtggg
     1021 agatagccaa aaaatatgag gatgaatttt tgactaattt ggatgaattg aacattgaaa
     1081 ggccgtggaa attaccaagg gcaacggatc atattgccga acaaattaat ttgatccaaa
     1141 aaattgaggc taacggctat acctataaaa ccagtgacgg gatttatttt gataccagca
     1201 aaatggttga ttatggcaaa atggctaatt taaatttgga aaaaataaaa gaaggggcaa
     1261 gggtggaggt aaatgaggaa aagaaacatc caactgattt tgcgttgtgg aaatttagcc
     1321 ccaaagatgt caaaagacag atggagtggg agagtccgtg gggagtgggt tttccgggat
     1381 ggcatattga atgcacggcc atgtcgacca aatatttagg ggaaaaattt gatattcata
     1441 ccggcggaga ggatcatatt ccggttcatc ataccaacga gatggctcag gcggaaggag
     1501 cttttggcca cgatttggtg agatattgga ttcataacgc ttttattact tttaagggaa
     1561 ataaaatcag caaatcaagt ggaggtttgt ggactatagc cgatttaaag aaaatggggt
     1621 ttgagccatt agcttttagg tatatggtat tgtcatcaca ttatcgaaaa ggaatggagt
     1681 tttcgataga gagtttaaag tcggctcaaa cagctttact aaaattaaga aaatatcggg
     1741 tggaagacgg aaaagattat gaagttaatc aggcattccg gcaagaattt atttccaaaa
     1801 tcagcgatga tttggcgatg ccagaagcgc tggcagtggt gtggaaatta atgaagtcag
     1861 atttgatagt ggaggaaaaa tgggcgacat tgttggatat ggacaaagta ttgggattaa
     1921 ggttgggaga gacggtattt gaggaaaaaa ttccggagga aattgcccaa ttggcacagc
     1981 agcggcagca agcaagggag aataaaaatt gggctgagtc ggatcggtta agagagctaa
     2041 taaaaagcca gggttattta gttgaagatt tagcaaactc ctgtaaaata agaaaaatat
     2101 aattttgtat tgtaaataga aacttattgt gttaatctaa taagctaatg aaaggaaata
     2161 aatatcgata ccagctggta ataattttaa atcccaagac agaaaacaaa gataagactt
     2221 gggaaaaagt caacggctgg ctgacaaaaa ataaaattga atcgacccag gaacatttgg
     2281 gattaaaaga gctggtttat gaaatcggca agaattctaa aggtgatttt tggactatgg
     2341 atttgacctc agagtcgcca atcaaacaaa aagacttaaa tttattacta aatcgggaac
     2401 ctaatattat tagatattta attttgaaaa aggagtaaaa catatgcc
//
LOCUS       DAOSHV010000067         2426 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_42458_length_2426_cov_4.795445, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000067 DAOSHV010000000
VERSION     DAOSHV010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42458_length_2426_cov_4.795445"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            21..656
                     /locus_tag="PKZ29_02580"
     CDS             21..656
                     /locus_tag="PKZ29_02580"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOG37785.1"
                     /translation="MEIIFRLISFLFLIISITLHEFFHSLAADYLGDPTPRSLGRLTL
                     NPKAHLDPFGTIILPLINIFTGIPTIGWAKPVPIDPFNFRHPRRDEIIVSLAGPFANL
                     TIAITTALILRFIPFISPIIYQLGALMILINISLFVFNLLPIPPLDGSHIFLNLLSPE
                     SSHKWAEAFDHYGFVLLIIFVFLPIYQGQTILSLIVSPLTKSIFHLLLPTL"
     gene            complement(625..858)
                     /locus_tag="PKZ29_02585"
     CDS             complement(625..858)
                     /locus_tag="PKZ29_02585"
                     /inference="COORDINATES: protein motif:HMM:TIGR03980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HOG37786.1"
                     /translation="MKKEKTKITKNILIGDLVANYPKLGRVLVEEYGLHCIGCMAAGM
                     ETLEQGAKVHGMSHKEIEEMVENLKELAKADEK"
     gene            complement(1382..>2426)
                     /locus_tag="PKZ29_02590"
     CDS             complement(1382..>2426)
                     /locus_tag="PKZ29_02590"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOG37787.1"
                     /translation="ENNYEVIFREVKAMTGMAIDMYVFSYRYMPASNRLLHRLSHKPA
                     VRELLEKSSRMNLPSLKRLINEVKPDLVLSTYFFHTHSLARWRKEKNKKFRLWTVAAD
                     PWTINPMSFIRDADLTLVYDEKSVEEGLAAGLSREQMLITGWWTRLGMYEKYDYKESR
                     KKLGFEDDRPVIFIGGGSLGTSALPKLLPLIMIIDKKVGLVINTGTDKLAYKMVEQYK
                     KMFSRFKKNDDVKIVHLGWIENMAEVLSGCDMVLGKAGPNFLFDTVACQKPFVAITHI
                     GGQEDGNIELIKKKKLGWVKERNKEILDFILSYLDRPKYFEKKYQENIKKEADKNKKT
                     LPLILEKIKKDVR"
BASE COUNT          642 a          633 c          301 g          850 t
ORIGIN      
        1 aatcaacatt tttttgaaat atggaaatta ttttcagact catttctttt ttatttttga
       61 ttatctctat cactcttcat gaattttttc acagtctggc cgccgactac ctgggcgacc
      121 ccactccccg atccctaggt cgactaaccc tcaaccccaa agcccacctt gaccctttcg
      181 gtactattat cttaccccta attaacatct ttactggcat ccctaccatc ggttgggcca
      241 aacctgtccc tattgatccc tttaatttcc gccatccccg tcgtgatgaa attatcgttt
      301 ccctcgccgg tccttttgcc aatcttacta ttgctattac cactgctctt attctccgat
      361 ttattccctt tatttcacca attatttacc aacttggagc tcttatgatc ctgatcaata
      421 tttccctttt tgtcttcaac ctcctcccta ttcctccact cgatggctct cacatttttc
      481 tcaatctcct atcccctgaa tccagtcata aatgggctga ggctttcgac cattatggat
      541 ttgttctatt aattatcttt gtctttttac ctatctatca aggacaaacc attctcagtc
      601 tgattgtctc accactaact aaatctattt ttcatctgct tttgccaact ctttaagatt
      661 ctctaccatc tcctctattt ctttatgact cattccatga accttagctc cttgttccaa
      721 agtctccatt cccgccgcca tacaaccaat gcaatgcaat ccatattcct ccaccaaaac
      781 tctccccaac tttgggtaat tagccaccaa atcccctatt aaaatatttt tagttatctt
      841 tgttttttct tttttcatat ccagattata acccgtttta aacctaaaat taacgcacta
      901 ataccgcaac taaccgaaca acttttccaa ataaactgac aaaccacttt attattgcta
      961 gacttaattg tcctatcaaa ggcatcgctt tgagaaggtg ttttttctaa ctaaacttct
     1021 acaaaggaga acttgtgact gtcggggctt cgttcctgcc agaccggttt tccccacgtt
     1081 tctttttagc gtggaaaagc cagtatcaaa gtcagccttt ggtgggacat tttttgagat
     1141 ttttaggtct aaatttggaa tcaaatgaca taattttgcc taaaaatcca atcccgatga
     1201 agctttagct aaatcgggat gagatgacat ttgttaacaa ttctctcagt cagcccgaaa
     1261 tcataatttt ggaacgtatt tgagcctgcg ttgcaggtct cattttccag tgaaaaaatt
     1321 gatgatgagg gttgatctga atgtttttac ttgatgaact tgatgaactt tacaaactta
     1381 atcatctaac gtctttcttt attttctcca aaatcaatgg caaagttttt ttgtttttat
     1441 ctgcctcttt tttgatattt tcctgatatt ttttctcaaa atatttcggt ctatccaaat
     1501 agctcaaaat aaaatcaaga atttctttat ttctttcttt cacccagccc aactttttct
     1561 ttttaatcaa ctcaatattg ccatcctcct gaccgccaat gtgggtaatc gccacaaacg
     1621 gtttttgaca cgccactgta tcaaacaaaa aatttggacc cgcctttccc aacaccatat
     1681 cacaccccga taacacttct gccatatttt caatccaacc caaatgaact attttgacat
     1741 catcattctt tttaaaccga ctaaacattt ttttgtattg ctccaccatt ttgtaggcta
     1801 atttatctgt ccccgtattg atcaccaatc ccactttttt atctataatc attatcaacg
     1861 gcaacaattt tggcaaagcg cttgtaccca aacttccccc accgataaat atcaccggtc
     1921 tatcatcctc aaacccaagc ttctttcttg actctttata atcatacttt tcatacatcc
     1981 ccaatcttgt ccaccaaccc gttatcagca tctgctcccg gcttaaacct gccgccaatc
     2041 cctcctccac gctcttttca tcataaacca acgtcaaatc agcatctctg ataaaagaca
     2101 ttgggttaat tgtccacgga tcagccgcta ccgtccacaa cctaaatttt ttgtttttct
     2161 cctttcgcca tcttgccaat gagtgagtat ggaaaaaata agtcgacaat actaaatccg
     2221 gcttgacttc atttatcaat ctttttagtg aaggcagatt catccgcgat gatttttcca
     2281 acaactctct cactgccggt ttatgagaca accgatgaag cagtctgtta gatgccggca
     2341 tatatctata ggaaaacaca tacatatcta ttgccattcc cgtcatggct tttacttccc
     2401 taaaaatcac ttcataatta ttttct
//
LOCUS       DAOSHV010000068         2272 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_46793_length_2272_cov_4.471809, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000068 DAOSHV010000000
VERSION     DAOSHV010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46793_length_2272_cov_4.471809"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1102)
                     /locus_tag="PKZ29_02595"
     CDS             complement(<1..1102)
                     /locus_tag="PKZ29_02595"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOG37788.1"
                     /translation="MNEENFLPWKYWLLRFGLALSLMILLTVVLKLNLIEGSYYTKAA
                     RSNKILESVIPAARADIVDRKGRVIAESVYQYFKLEEGNKIYQSSGKFDGYKFEGKDL
                     AYEVRREYPYGESMSMITGYVGKVSDEDIKKDSCQIRLKKTDIIGRGGFEAYNDCSIR
                     GKNGKRIVEVDALGQYVRELGREEPEIKPKISLSIDAYWQDKIYKMLEGKKAVVIMSQ
                     PDTGKIISLVSSPAFDANDFANQNNQKINDYLNNKDGLPLLNRAVSARYHPGSVFKIV
                     VATAGLESKVIDKTSLIEDTGVLKVGDYSYKNWLWSKSGGTDGMVDIVKALKRSNDIF
                     FYKLGEMLGVDRIKNWSEKYGFGARTGVEITGE"
     gene            complement(1104..2186)
                     /locus_tag="PKZ29_02600"
     CDS             complement(1104..2186)
                     /locus_tag="PKZ29_02600"
                     /inference="COORDINATES: protein motif:HMM:NF010539.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOG37789.1"
                     /translation="MKLIKAVGQKISKLTQMFSWDIGIDLGSNNTLIYLKERGVIIDE
                     PTMLARQKKKRWTGLSAPKSDTKRPIAFGYKAKSMWNREPKQIEVVSPIKNGIITDLE
                     GLEGLMSYYFKLIYEVPSKYPKIFKPRVIVGVPSFINQVQKRAIKSVLMAAGAREVVL
                     IEEVVLASIGLNLPADVSGGLVIVDVGGGKTEASVVSMGGVVVGRGIKTAGEELDNAI
                     VNYIKMKYGMLVGPNSAEKIKIDVETNNLVRGRDLETGLPRSVKLTKSEINEAVSLEL
                     TKIVKLVKAVLDETPPELMEDVLKRGIVLVGNGAKLKNLAEMIESETKIYTVVAEDPG
                     MAIIRGAGLLVEERKLLDQVRRVALG"
BASE COUNT          579 a          692 c          268 g          733 t
ORIGIN      
        1 cttctccggt tatttccacc cccgtcctgg ccccaaaacc atatttttcc gaccagtttt
       61 ttatccgatc cacccccagc atctctccta atttataaaa aaagatgtca tttgaccttt
      121 ttagcgcctt aacaatatcc accattccat cagttccccc gcttttgctc cacaaccaat
      181 ttttgtaaga ataatcccct actttcagca cccccgtatc ttcaatcaaa ctggttttat
      241 caattacttt cgattccaat cctgccgttg ccaccacaat tttaaacacc gatcccggat
      301 gataccgggc cgacaccgcc cggtttaaca gaggcaagcc gtctttatta ttcaaataat
      361 cattaatttt ttgattattt tgattagcaa aatcattagc atcaaatgcc ggacttgata
      421 ccaaactaat tattttaccc gtatctggct gactcataat cactaccgcc tttttcccct
      481 ccagcatttt gtaaatctta tcctgccaat aagcatcaat cgacagactg atttttggtt
      541 taatttccgg ttcttcccgc cccaattccc gcacatactg tcccagcgca tccacttcca
      601 caattctctt gccgtttttc ccccttatac tgcagtcgtt ataagcttca aatcctcccc
      661 ggccgattat gtcagttttt ttaagtctga tttgacaact atcttttttg atatcctcat
      721 ccgacacctt ccccacataa cctgttatca tcgacatcga ttctccataa ggatattccc
      781 gtctaacctc ataagccaaa tccttgccct caaatttata accatcaaat ttacccgaac
      841 tttgataaat tttgttaccc tcctccagct taaaatattg ataaacactt tcagctatca
      901 ctctgccttt tctatcaaca atgtccgctc tagctgccgg aatcacactc tccaaaattt
      961 tatttgacct ggccgctttt gtataataac tcccttcaat taaattaagt ttcaacacta
     1021 ctgtcagcaa tatcatcaaa cttaatgcca gtccaaatct cagcagccaa tatttccaag
     1081 gtaaaaaatt ttcttcattc atactaaccc aatgccaccc tccttacctg atccaataac
     1141 tttctttcct ctaccaacaa tcctgcccct ctgataattg ccatccccgg atcctctgcc
     1201 acgactgtat aaatctttgt ttcactttca atcatttctg ccaaattctt cagtttagct
     1261 ccattcccca ccaacacaat tccccgcttc aacacgtctt ccatcagctc cggcggcgtc
     1321 tcgtccagca ccgcttttac cagtttgaca atcttggtca actccaaact caccgcctca
     1381 ttaatttctg acttcgtcag tttcactgat cttggcagac cggtttccaa atcccgcccc
     1441 cgcaccaaat tatttgtctc tacatcaatt ttgattttct cagcactgtt tggacctacc
     1501 agcatcccat acttcatttt tatataattt acgatggcgt tgtccagttc ctccccggct
     1561 gtttttatcc ccctgccgac caccactcca cccatcgaca ccacgctagc ttcagttttt
     1621 cccccgccca catccacaat taccagccca cccgacacat ctgccggcag attcaatcct
     1681 attgacgcca acaccacttc ctctatcaac accacttctc tggctcccgc cgccatcagc
     1741 accgatttta tagctctttt ctgaacttga ttaataaaac ttggcactcc cacaatcact
     1801 ctcggcttaa aaattttggg gtattttgat ggcacttcat aaataagttt aaaataataa
     1861 gacattaaac cctccaaccc ctccaaatcg gtaattattc cgttcttaat cgggctcacc
     1921 acctcaatct gcttcggttc tcgattccac atcgacttgg ctttgtaacc aaaagcgatc
     1981 ggtcttttgg tatctgattt tggcgccgac aatcccgtcc atcttttttt cttctgccgc
     2041 gccaacatcg tcggctcgtc aattatcact cctctttctt ttagataaat cagggtatta
     2101 ttacttccca aatcaatccc aatatcccag ctaaacattt gggttaattt gcttattttt
     2161 tgccctactg ccttaattaa cttcactcta aaattatagc aacttaaacc aacctcggtt
     2221 acaatataaa ctatccatga aacgccttga tcaaatcatc cgttttcttt at
//
LOCUS       DAOSHV010000069         2268 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_46909_length_2268_cov_4.607320, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000069 DAOSHV010000000
VERSION     DAOSHV010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46909_length_2268_cov_4.607320"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1386
                     /locus_tag="PKZ29_02605"
     CDS             <1..1386
                     /locus_tag="PKZ29_02605"
                     /inference="COORDINATES: protein motif:HMM:NF012731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HOG37790.1"
                     /translation="KKALDVIEEVIKTIRGTKPDEDPKIKLMTKFGFTDLQAQAILDM
                     QLRKLSGLERQKIDEEYNQVEKTISELTAIITKPEKMIAALKSENKEIIEKYSDSRRT
                     KIYIRGLEDFSDEDLIPNEPALVTLTKTGYIKRVPKETYQTQKRGGIGVIGMTTKQED
                     EIACLLSVNTHNDLLFFTNRGRVFKTKVWDIQEGNRQAKGQAVVNLINILQGESVHTV
                     LAIDTEDVKKNNHSQYILLATKNGVIKKTSVEKFKNIRQSGLAAIKLDADDQLAWAKV
                     TDGQSHIFLVTKHGKCIRFSESDTRPMGRHTRGVRGILLKESDHLVSMDIIPPAIEKP
                     KDKLALKPFRHLLVVTENGVGKRSDVYLYPLQKRGGIGVKVANLTAKTGNIAAAQVVS
                     ETVEQVILVSKKAVTIKLPIKNIPVLSRNTKGVILMRFKSNEDKLTAMTIIENTNQIE
                     EVKPTPAKKNN"
     gene            complement(1653..>2268)
                     /locus_tag="PKZ29_02610"
     CDS             complement(1653..>2268)
                     /locus_tag="PKZ29_02610"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOG37791.1"
                     /translation="PKIKDHSLKFLDEQQIYRLLNQPLNSSKNGLRDRSILELLFSTG
                     LRVSEMVALDREQVNLKTREFGVIGKGGRSRVVFVTKQAAEWIDRYLKSRTDVYKPLF
                     IRDLRPKEIALADDKVRLTARSVQRLVKKYVRQANLPVAATPHTLRHSMATDLLQRGA
                     DLRSVQELLGHKNIATTQIYTHVTDARLKEVHEKFHVGNAKKEE"
BASE COUNT          694 a          544 c          409 g          621 t
ORIGIN      
        1 aaaaaagccc ttgatgttat cgaagaggtt ataaaaacca tccgcggcac caagcccgac
       61 gaagatccca aaatcaaatt aatgactaaa ttcggcttta ccgatcttca agctcaggct
      121 attttagata tgcagcttcg taaactttca ggtctggaac ggcaaaaaat agacgaagaa
      181 tataatcagg tcgaaaaaac tatttccgaa cttaccgcca ttatcaccaa accggaaaaa
      241 atgattgccg ctctcaagtc cgagaacaaa gaaatcatcg aaaaatattc cgattcccgc
      301 cggaccaaaa tttatatccg tggactcgaa gattttagcg acgaagacct aattcccaac
      361 gaaccggccc tagttaccct taccaaaacc ggctacatca aacgggttcc taaagaaact
      421 tatcaaactc aaaaacgcgg tggtatcggt gttatcggta tgaccaccaa acaagaggat
      481 gaaatcgctt gtttgctctc cgtcaacact cacaacgacc ttctcttttt taccaatcgc
      541 ggtcgggttt tcaaaaccaa ggtttgggat attcaggaag gcaaccgtca ggccaaaggt
      601 caggctgttg ttaacctcat caatattctc cagggagaaa gtgtccatac cgttttagcc
      661 attgataccg aggatgttaa aaagaataat cattctcaat acattctact ggcgaccaaa
      721 aatggggtta tcaaaaagac ttctgtagaa aaatttaaaa atatccgtca aagcggcctg
      781 gctgccatca agctcgatgc cgacgatcag ttggcctggg ccaaggtcac cgacggtcaa
      841 agccacatat ttttggtcac taaacacggc aaatgtattc gttttagcga atccgacacc
      901 cgtcccatgg gtcggcacac ccgcggtgtt agaggtattc tactaaaaga aagtgatcat
      961 ttagttagta tggatattat tcctccggct atcgaaaaac ccaaagataa attggctctc
     1021 aaacctttcc gacatcttct ggttgtcacc gaaaacggtg ttggcaaaag aagcgatgtt
     1081 tatctctatc ccctccaaaa acggggcggt atcggcgtca aggtagctaa tctgactgcc
     1141 aaaaccggaa atatcgctgc cgcccaagtg gtttctgaaa ccgtcgagca ggtgattttg
     1201 gtctccaaaa aagccgtcac catcaagctc cccatcaaaa atattccggt tctaagccga
     1261 aatactaaag gagttatttt aatgcgtttt aagtcaaacg aagacaaact aactgccatg
     1321 accatcatcg aaaacaccaa ccagattgaa gaagtcaaac cgacaccagc caaaaaaaat
     1381 aactaagctg tatttttgcc gccaaagtct aatattataa gagatacacc gtcagtaagt
     1441 agtttttagc agagtagggg actagttgct aataagaaac attattttag ttaaaccctg
     1501 ttttagggca aaaacaattt gtcaagtcta tttttttttc aacctgtttt ttattcccaa
     1561 atctctaata aatttgcctt actataggtt atttaacccc aaaaatataa ccaaaactat
     1621 ttgtttacca gtaggggagt agttttgttt ttttattcct cttttttggc attccccaca
     1681 tgaaacttct catgaacctc tttcaacctg gcatcagtca cgtgagtata aatctgagtc
     1741 gtcgcaatat ttttatgacc cagcagttct tgaaccgatc ggagatcagc cccccgctgc
     1801 agtaggtcag tcgccatcga atgccgcaaa gtatgcggtg tggctgccac cggcaaatta
     1861 gcctgtcgca catatttttt taccaatctc tgaaccgacc ttgccgtcaa tcttaccttg
     1921 tcatccgcca aggcgatttc ctttggtctt aagtctctaa taaataaagg cttgtaaaca
     1981 tcagttcttg attttagata tctatctatc cactcagccg cctgcttggt cacaaacacc
     2041 actctcgacc ttccgccctt gccgataacc ccaaattccc gtgtctttaa atttacctgt
     2101 tccctatcca aagccaccat ttccgacacc ctcaacccgg tcgaaaataa caattccaaa
     2161 attgaccggt ctcttaggcc atttttactt gaattaagcg gttgatttag taatctgtat
     2221 atttgttgtt catccagaaa ttttaaagaa tgatccttaa ttttcggc
//
LOCUS       DAOSHV010000070         2199 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_49132_length_2199_cov_3.764925, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000070 DAOSHV010000000
VERSION     DAOSHV010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49132_length_2199_cov_3.764925"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            139..543
                     /locus_tag="PKZ29_02615"
     CDS             139..543
                     /locus_tag="PKZ29_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37792.1"
                     /translation="MKKGQALIESVVAVGVVALVMTAAIALVVLSTSHRRNNFDRRKA
                     NELANYVEETVINESQSDVANFWSNLNGGSCGDVDFAGYTCNYSFTVLTQPTHTNCGS
                     GAPPRCAELIVTITWPGKTQQNLTVRRFFSRR"
     gene            540..1040
                     /locus_tag="PKZ29_02620"
     CDS             540..1040
                     /locus_tag="PKZ29_02620"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOG37793.1"
                     /translation="MKKAFTLLEIVVVVAILGLIMTMLSGSLGMSFKLKNLTETNERL
                     TSKANFILGELKKNILEARAETIVCSSSRTQLEYITLGGATVILSCDIGSTRIASQSG
                     EVVFNLSDKDVIPRTCNSFVNCRLNTDTSMVESVGITLDLVTTVGHEGVGSSGRFYQT
                     LSPRLW"
     gene            1112..1882
                     /locus_tag="PKZ29_02625"
     CDS             1112..1882
                     /locus_tag="PKZ29_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly PilX N-terminal domain-containing
                     protein"
                     /protein_id="HOG37794.1"
                     /translation="MKKNRQAQVAIIALLVSAVVMVVGLTFSKMTTVETRIDTNEEAL
                     RDAFNAAESGIDYYLRTGSQTYTVPTSTAKAEISVRSLGNEVVLDFGEYTPEGESEFY
                     WLVNHTATGLGSNYFGGNQITVRHKGIDGSVVINVFFRRGSGFGVKRYGYNFSNNADK
                     RVNNFIDAIGNQSVTISPIPTNPVLMTVTPIFSGANLSIASVGVTFPAQGEEISSVGK
                     VGEIGAGQVSKKVVIQQRYRLPNFFLDALSSEASVLSN"
     gene            1906..>2199
                     /gene="pilM"
                     /locus_tag="PKZ29_02630"
     CDS             1906..>2199
                     /gene="pilM"
                     /locus_tag="PKZ29_02630"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HOG37795.1"
                     /translation="MSQMIGVDVGLKSIKAVILEKNESNYLLVGIGEIANPGTDWIGS
                     QTGGKNLEEVAKAIKTLLKDLKINTKLAATALPEHETVSRLVSLPPLKETEIRD"
BASE COUNT          695 a          272 c          566 g          666 t
ORIGIN      
        1 agatgcagtt ggaatgatgt atagtgctgg ggaaacagca gctattactt tgagtttgaa
       61 agagactcaa aatatgtcag taccgattat tattgatgct atggggcaga tcagtcaagg
      121 ggaggtggtt caaaattgat gaagaaagga caagccttaa ttgagtcagt ggtagcagtc
      181 ggagttgtgg ctttagtgat gacggcggca attgctttag tagtgctatc gacttctcac
      241 cggaggaata attttgatag aagaaaggct aatgaattag ctaattatgt agaggagact
      301 gttattaacg agagtcagag tgatgtagcg aatttttgga gtaatttgaa tggaggtagt
      361 tgtggagatg ttgatttcgc tggatatact tgtaactaca gttttacagt attaacgcaa
      421 ccgactcata ccaattgtgg cagtggagca ccaccaagat gtgctgagtt gatagtcaca
      481 atcacttggc ctggaaaaac tcaacaaaat ttaacagtta ggcgtttttt tagtcgaaga
      541 tgaaaaaagc ttttacttta ttggaaattg tggtggtagt ggcgatttta gggttgatta
      601 tgaccatgtt gtcagggtca ttgggaatgt cttttaaatt aaagaatttg acagagacta
      661 atgaaagatt aactagtaaa gctaatttta ttttagggga gttgaaaaaa aatattttag
      721 aagctagagc ggagacaatt gtatgtagca gcagtcggac tcaattagag tatatcactc
      781 ttggtggtgc cacagtaatt ttgagttgtg atataggcag tacacgaatt gcatctcagt
      841 ctggggaagt ggtgtttaat ttatcagaca aggatgtgat tccaagaact tgtaatagtt
      901 ttgtgaactg tcgattaaat acggatacta gcatggtcga gtcggtggga ataactttag
      961 atttagtgac cacagttgga catgaggggg tgggtagtag tggtcgcttt tatcaaactt
     1021 tatctccccg attatggtga tacaatttga tagatattga atttacctat ggaagttgct
     1081 aaagttttat tagattttaa aagatgaggg aatgaaaaag aatagacaag cgcaagttgc
     1141 gattattgct ttattagttt cggcggtggt tatggtagta gggttaactt tttctaagat
     1201 gactactgtt gaaacgcgaa tagataccaa tgaggaagcg ttgagagatg cttttaatgc
     1261 ggctgagtca gggattgatt attatctgag aactggatct caaacgtata cggtaccaac
     1321 atcaactgca aaggcggaaa taagtgtgag gagtttgggt aatgaagtag tgttagattt
     1381 tggtgaatat actccagagg gagagagtga attttattgg ttggttaatc acacggcaac
     1441 cggtttaggg agtaattatt ttggaggtaa tcaaattaca gttagacaca aaggaataga
     1501 cggttcagta gtaatcaatg ttttctttag aagaggaagt ggttttggag taaagcgtta
     1561 tggttataat tttagtaata atgctgataa aagagtcaat aattttattg atgctattgg
     1621 taatcaaagt gttactatta gtccgatacc aactaatcca gtattaatga cagtgacacc
     1681 gatttttagt ggagctaatt tatcgattgc gagtgtcgga gtcacttttc cagcacaagg
     1741 agaggaaata agttcagtag gaaaggtggg tgagattggt gctggtcagg tgagtaaaaa
     1801 agtggtgatt caacaaagat atagattgcc taattttttt ctagatgcac ttagctctga
     1861 ggcctcggtc ttgagtaatt aaaggaaaat acctatactg aatatatgag tcagatgatt
     1921 ggggtggatg ttggcctcaa aagtattaaa gcggtaattt tggaaaaaaa tgagagtaat
     1981 tacttattgg ttggcatcgg tgaaatagcc aatccgggga cggattggat tggttctcaa
     2041 acgggaggaa aaaatttgga ggaagtagct aaggcgatta agacattgct aaaagatcta
     2101 aaaataaaca ctaaattagc agcgacagct ttgccggaac atgagactgt atcaagattg
     2161 gtttctttac cgccacttaa ggagacggag attcgagat
//
LOCUS       DAOSHV010000071         2122 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_51809_length_2122_cov_2.873730, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000071 DAOSHV010000000
VERSION     DAOSHV010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51809_length_2122_cov_2.873730"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="PKZ29_02635"
     CDS             complement(<1..383)
                     /locus_tag="PKZ29_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37796.1"
                     /translation="MDTNQLFSNLKSQFPNLEIYQHHPLAPYTTVNIGGPADIFLHST
                     SSHQLTQILQYLRSINYDLISILGNGSNTLIADSGIRGLVIKNSSDQFTLLDSDSPTT
                     TASTSISAHRTENDPSKYLNFSQID"
     gene            860..1456
                     /gene="ruvC"
                     /locus_tag="PKZ29_02640"
     CDS             860..1456
                     /gene="ruvC"
                     /locus_tag="PKZ29_02640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOG37797.1"
                     /translation="MILGIDPGLANTGWAVLDLRSQMSDVSLSNSSNSSSLSSSSGCK
                     VKRFKNKTLIDQEGLVSEFELVACGCLRTKIGEESAKRLGKIYRELERLIKKYKVEAI
                     ALESLFFAKNAKSALKVAEAIGVIKVCGQKNGVEVVEFTPLQVKTALVGYGRAEKEQV
                     EIMVRNFLGLEENISPSHASDAVAVGLTYLFTNRDLVV"
     gene            1487..>2122
                     /locus_tag="PKZ29_02645"
     CDS             1487..>2122
                     /locus_tag="PKZ29_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37798.1"
                     /translation="MEVKNETAPIEIKSLAEKIEMRISQSRVDRLVEYVMGMGLEDSK
                     LIDRLETDQMKVRAKYGLPDRKYRFANPSEYEAFLRELADGNGVRVRSKSDCGNFFEK
                     YPFAGAVYLDQEKTVGVNIDKKSQDNYLKSLLLLEHELIHALQYKYYPGMPVEIQEYE
                     AYVAGWNIDYLRENPEKVSDIFGFFVMGSVKHWYQETSENIHETLEPVWDSP"
BASE COUNT          667 a          234 c          604 g          617 t
ORIGIN      
        1 tagtcgattt gggaaaaatt aagatatttg ctgggatcgt tttcggtgcg gtgggcagaa
       61 atggaagtgg aagcggtagt ggttggcgag tctgagtcga gaagagtaaa ttggtcagag
      121 ctatttttga taaccagacc gcggatgccg gagtcggcga taagagtatt ggaaccgttt
      181 cctaaaatag atattagatc gtagttgata gatcgtagat attgtaggat ttgggttagt
      241 tggtgggaag aggtggagtg gagaaaaatg tcggcaggac cgcctatatt gacggtggtg
      301 tagggggcga gcgggtgatg ttgataaatt tcaaggttgg ggaattggga cttgagattg
      361 gagaaaagtt ggttggtgtc cataagagga ttatagctta atttagatct cagatgtcag
      421 atctcagatc tcagtttttc agatctcagt ttttcagatc tcagatgtca gatccgctgg
      481 tgggcggacg ggtcttcagg aaaacaaaac ccctctgtcc cgcagtggcg ggacatctcc
      541 ccttaacagg ggaggaagat tctagataca agatttaaga atctgccggt gggtggacag
      601 gtatcagatc tcagtttttc agatctcagg aaaacaataa aacagattta aggataaaaa
      661 atagtgaatt ttgaacctga aatatgtctt ataaaataga tttttggaga tagtaaaaaa
      721 atatgggatg gtgatgtgga aaagtggaga aaatgagttt tatcctataa gttgtaaagc
      781 aatttgaaaa ttttaataaa gtgaagattt gggatgtttt atggttgtgg tgatagagtt
      841 ttaactgtta tgattggtta tgattttagg aattgatccg ggattggcga ataccgggtg
      901 ggcggtatta gatcttagat ctcagatgtc agatgtcagt ttgtcaaatt catcaaattc
      961 atcaagtttg tcaagttcat caggttgtaa ggtgaaaaga tttaagaata aaacgttgat
     1021 tgatcaagag gggttggtaa gtgaatttga attggtagct tgcgggtgtt tgaggacaaa
     1081 gattggggaa gagtcggcga aaagattggg aaaaatttat agggaattgg aaagattgat
     1141 taaaaagtac aaggtggagg cgatagcttt agaaagtttg ttttttgcca aaaatgccaa
     1201 gtcagcactg aaagtggcgg aagcaattgg ggtgatcaag gtttgcggtc agaaaaatgg
     1261 agttgaggta gtggagttta cgccattgca agtgaaaaca gctttggtgg gttatggtcg
     1321 ggcggagaaa gaacaagtgg aaataatggt gcgtaatttt ttggggttgg aagaaaatat
     1381 ttcaccgtct cacgcttcgg atgcggtggc agtaggatta acttatttgt ttactaatcg
     1441 ggatttggta gtttgatatg gtttggttat ggtgagataa tgatttatgg aagttaaaaa
     1501 tgaaacagct ccgatagaaa taaaatcgct ggcagaaaaa atagagatga gaatatctca
     1561 gtcgagagtg gatcgattgg tggaatatgt aatggggatg ggtttggaag attcaaaatt
     1621 aatagacaga ctggaaactg atcaaatgaa agttcgagcc aaatatggtt tgcccgacag
     1681 aaaatacaga ttcgccaatc ctagtgaata tgaagctttt ttgagagagt tagcagatgg
     1741 taatggagtc agagtgagat caaaaagtga ttgtggtaat ttttttgaaa aatatccttt
     1801 tgcgggagcg gtttatttag atcaagaaaa aacagtgggg gtaaatatag ataaaaagag
     1861 tcaagataat tatttaaaga gtttgttgtt attggaacat gagttgattc atgctttgca
     1921 atataaatat tatccgggga tgccggtgga gatacaggaa tatgaggctt atgtggccgg
     1981 ttggaatata gattatctaa gggaaaatcc agaaaaggtg agcgatattt ttggattttt
     2041 tgtaatgggt tcggtaaaac attggtatca ggagacaagc gagaatatac atgaaacctt
     2101 ggagccggtt tgggacagtc cg
//
LOCUS       DAOSHV010000072         1998 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_56709_length_1998_cov_3.984560, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000072 DAOSHV010000000
VERSION     DAOSHV010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56709_length_1998_cov_3.984560"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(325..1203)
                     /locus_tag="PKZ29_02650"
     CDS             complement(325..1203)
                     /locus_tag="PKZ29_02650"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOG37799.1"
                     /translation="MITKAVIAAAGRGTRFLPVVKSYPKELVPILSKPNIQYLLEEAV
                     GAGITNIAIIIRHGENRLQSYFQPDPELESYLKSTNKLSYIDSLDQLLSQAKITFIEQ
                     PVSLPYGTGSPLLTAQDFVGSDPFIYIYGDDLTVEPVPGQFLTKMMTVFDQYHPDEII
                     AVKDVGVAEIARYGSAQYVEDPNYPHRISAMLEKLPADQAPSTFAQGGRFLVTSKIFP
                     ILKNQGLSRDGELWFSDASNTLAQSGIVLTQAFDGSEDWLTTGDPLRWLEANITLALN
                     DPKYHDDLIKFIKNIK"
     gene            complement(1298..>1998)
                     /locus_tag="PKZ29_02655"
     CDS             complement(1298..>1998)
                     /locus_tag="PKZ29_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37800.1"
                     /translation="SIEKYFSPDPELEKYLTDTNKLEYLDSLKSIWDKAKLTFIPQPT
                     NLPYGNGSPVLAAKNFIGTNDFVYMFGDDLTVEPIPGQFLSKMINTFNQYSPASVIAV
                     KDVGLEEISRYGSAQYMDDPNYPHRISAMLEKLPADQAPSTFAQGGRFVISNKIISVL
                     ENQPTGQGNELWLADAQNYLAKNDVVLTQAFEADADWLTTGDPLRWLIANITLALNDP
                     KYHDDLTKFIKNIK"
BASE COUNT          569 a          323 c          526 g          580 t
ORIGIN      
        1 acataaaaat atctcctttt taatttttaa tggggaatta ataaactagt taacactact
       61 attgtagcat aataaggctg attgtcaaga ttataggata tgatagttta taaagttgaa
      121 agtttatcaa gttcatcaag taaaaacatt cagatcaacc cttatcatca atttttccac
      181 tggaaaatga gatctgcaac gcaggcttaa atacgttcca aaatgatgat ttcgggttga
      241 ttagtaggat tatattttta ggcaaattat gtcattatta cttgaaattt agacctaaaa
      301 atctcaaaaa ctgtggtttt gagattattt tatgttttta ataaatttaa ttagatcgtc
      361 gtgatatttg gggtcgttga gggcgagggt gatattggct tcaagccagc gaagcgggtc
      421 gccggtagtg agccagtctt cggaaccgtc aaaggcttgc gtaagaacaa tgcctgattg
      481 ggctaaagta ttgctggcgt cagaaaacca aagctcaccg tcgcgggaga gaccttgatt
      541 tttgagaatg ggaaaaattt tggaagtaac caaaaatcgg ccgccttggg caaaagtgga
      601 tggggcttgg tcggcgggaa gcttttctag catggcagaa attcgatggg ggtaatttgg
      661 atcctccaca tattgagccg aaccgtagcg ggcaatttcg gccacaccaa catctttgac
      721 ggcaataatt tcgtcagggt ggtattgatc aaaaacagtc atcattttag ttaaaaactg
      781 accggggacc ggttcaactg taagatcgtc accataaata tagataaacg ggtcggaacc
      841 gacaaaatct tgggcggtga gcaggggtga accggtgccg taggggagtg aaacgggttg
      901 ttcaataaag gtgattttgg cttgagacag aagttgatca agactatcaa tataggatag
      961 tttattggtg gatttgagat acgattctag ttcggggtca ggttgaaagt aagattggag
     1021 ccggttttcg ccatgacgga tgatgatagc gatgttagta atgccggcac cgacagcttc
     1081 ttcgagaaga tattgaatgt ttggtttgct aagaataggg actaattctt tgggataaga
     1141 tttgacgacg ggtaaaaatc tggtgccgcg gccggcagcg gcgataacgg ctttagtaat
     1201 cataagtttt gtttaaatct cttatcataa attttctctc tggaaaatga ggtctgcgac
     1261 gcaggctcaa atacgttcga aaattatgat ttcgagatta ttttatattt ttaataaatt
     1321 tagtcagatc gtcgtgatat ttgggatcgt tgagggcgag agtgatgttg gcaataagcc
     1381 agcgaagcgg gtcgccagtg gtaagccagt cggcatcggc ttcaaaagct tgggttaaaa
     1441 cgacatcgtt tttggcgagg taattttggg cgtcagccag ccaaagttcg ttgccctgtc
     1501 cggtgggttg attttctaaa accgaaataa ttttgttgct gatgacaaag cggccgccct
     1561 gagcaaaggt cgacggagct tggtcggcgg gaagcttttc tagcatggca gaaattcggt
     1621 gaggataatt tggatcatcc atatattggg ctgaaccata acggctgatt tcttccaggc
     1681 cgacatcttt gacagcaatg acggaggcgg gggaatattg gttaaaagtg ttaatcattt
     1741 tggataaaaa ctgacccggg atgggttcga ccgtaaggtc gtcgccaaac atataaacga
     1801 aatcgttggt gccgataaaa tttttggcag ctagaaccgg ggaaccgttg ccatagggaa
     1861 ggttggtagg ttggggaatg aaggtgagtt tggctttgtc ccagatggat ttgagactgt
     1921 ctaaatattc tagtttgttg gtatcggtaa gatatttttc cagttccggg tcgggagaga
     1981 aatatttttc gatagagt
//
LOCUS       DAOSHV010000073         1964 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_58240_length_1964_cov_3.187533, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000073 DAOSHV010000000
VERSION     DAOSHV010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58240_length_1964_cov_3.187533"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="PKZ29_02660"
     CDS             complement(<1..494)
                     /locus_tag="PKZ29_02660"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOG37801.1"
                     /translation="MLKGPIVNTGFQILGKVVTVGLSLVTTAVLTRKLGSSAYGNYLL
                     ITSVWLLLDAVADFGSRVIGVREAAIETGEKKNWVYIQIAWFRLMAALVGLIIGLGII
                     WWWPGFDGIEIEALVALLMIGLTSIAGSLEIIFQTEMKMGRKVWMDILFPLLFTVVLL
                     TTEE"
     gene            complement(517..1752)
                     /locus_tag="PKZ29_02665"
     CDS             complement(517..1752)
                     /locus_tag="PKZ29_02665"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOG37802.1"
                     /translation="MKEGLEKISKIILFWLLLLIPTQLGKHFWFDWSLVWGMRVDYFS
                     PTVYVIDILFLGLFLIEIYRQGVDWKSIKKNWWIVGLVVVNLLIASNRWVAGYRWLRW
                     GQLMWFGGYVIKNKRWVEIYLGKVIPYWLIVEGFLVLAQIAKGGSLNGIWWWLGERKF
                     DFSTIGVAQMSVANVGLVRGYGTFSHPNSLAGFLLVSLLWWLKYYRQLFLISWGKLKW
                     WIVFWSGLMGILLSGSRTIWLLTLVAILMWWRFNYRNSKERIVFWLLVGVGLFLGLEM
                     IDFNYPLANFLSGWDENGMIKRGQLNLAAVEMIKSSPWFGVGLGNFLVKLPQFQKNNQ
                     IFWFQPVHNVILLLVSETGLLGLVAVIWGLRKKIDWQNWSKLTWLILGVIFISGMVDH
                     YWLTLPQNMWLLTLVIALI"
BASE COUNT          665 a          587 c          160 g          552 t
ORIGIN      
        1 atctcctcag tcgtcaataa taccactgta aataataacg gaaataaaat gtccatccat
       61 actttccgac ccattttcat ctctgtttga aaaataattt ccaaacttcc cgctatcgac
      121 gtcaatccga tcattaataa cgctaccaat gcttctattt caattccatc aaatcccggc
      181 caccaccaaa ttattcccaa accaattatc aaacccacca atgccgccat caatctaaac
      241 caagctatct gaatatacac ccaattcttc ttttctcccg tctctattgc cgcttctcta
      301 acccctatta ctcgactacc aaaatcagcc accgcgtcca aaagtaacca taccgaagta
      361 attaacaaat aatttccata agcactcgaa cctaattttc tggtcaacac cgccgttgtc
      421 accaaactta atcccactgt caccacttta cccaatattt gaaacccggt attaactatt
      481 ggtcctttta acataaatct attatctcac cctagactaa attaacgcta tcaccaacgt
      541 caacaaccac atattttgag gtaaagtcaa ccaataatga tctaccattc cactaataaa
      601 tatcactccc aatattaacc atgttaattt gctccaattt tgccaatcta ttttttttct
      661 taatccccaa attactgcca ccaatcccaa caatcccgtt tctgatacca acagtagtat
      721 cacattatga accggttgaa accaaaaaat ctgattattt ttctgaaatt gaggcaattt
      781 taccaaaaaa ttacccaaac ccaccccaaa ccatggtgaa ctcttaatca tctccactgc
      841 cgccaaattt aattgacctc ttttaatcat tccgttttca tcccaccctg acaaaaaatt
      901 tgccaacgga taattaaaat caatcatttc taatcctaaa aataatccca ctcccaccaa
      961 caaccaaaaa actattcttt ctttactatt acgataatta aatctccacc acatcaatat
     1021 cgccaccaaa gtcagtaacc aaattgtccg acttcctgac aataatatcc ccatcaaccc
     1081 cgaccaaaac actatccacc atttcaattt tccccaactt attaaaaata actgtcgata
     1141 atatttcaac caccacaata aactcaccaa caaaaaccct gccaaactgt tcggatgaga
     1201 aaaagttcca tatcctcgca ccaatcccac attggccacc gacatttgtg ccaccccaat
     1261 agtactaaaa tcaaattttc tttcacccag ccaccaccat attccattca aacttccccc
     1321 tttggcaatt tgcgccaaca ccaaaaaacc ctccactatc aaccagtagg ggatcacctt
     1381 tcccaaataa atttctaccc accttttatt tttaattaca taccctccaa accacatcaa
     1441 ttgaccccac ctcaaccatc gataccctgc cacccatcta ttactggcta tcaacaaatt
     1501 aactactacc aaccctacta tccaccaatt cttttttatt gatttccaat ctactccttg
     1561 tcgatatatt tcaatcaaaa acaatcctaa aaacaaaata tctatcacat aaaccgtcgg
     1621 tgaaaaataa tccactctca ttccccaaac caagctccaa tcaaaccaaa aatgcttacc
     1681 caactgagtt ggtatcaaca acaacaacca aaacagaatt attttactaa ttttttccaa
     1741 accttctttc acaccccaat cttaccacaa aatcccctaa acctacccaa acaccaccag
     1801 caggggagtc aactatcgtt ttacccatcc gcccaccaac ggattcttac atcttaaatc
     1861 ttaaatcttt tttattgttt tcctgatatc tgagatctga aaaactgaaa tctaatttta
     1921 gcgccgacaa tattttttcg cccgcaagcc gctgaggcct tctg
//
LOCUS       DAOSHV010000074         1951 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_58817_length_1951_cov_4.684072, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000074 DAOSHV010000000
VERSION     DAOSHV010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58817_length_1951_cov_4.684072"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..783
                     /locus_tag="PKZ29_02670"
     CDS             <1..783
                     /locus_tag="PKZ29_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOG37803.1"
                     /translation="VLMLAKYFSKRHIEIANIRHIIVSGVYAFFPFVAIMLQPDFGSA
                     LIIFMIWLGMILVSGVSRKHLAWLGLAAIIFSVGAWMFVLQDYQKARIMTFIHPLADV
                     RGSGYNAFQSTIAVGSGQLTGKGVGYGTQSRLKFLPEYQTDFIFAAFAEEWGFFGVLI
                     FFFLFGLIIWRILATAMKGATNFEILFGLGIAVMFMSHLIIHVGMNIGILPVTGLPLP
                     FLSYGGSHLLAEFMGLGILMGMRRYSLAYHRDDVKNEFIGPQ"
     gene            1155..1778
                     /locus_tag="PKZ29_02675"
     CDS             1155..1778
                     /locus_tag="PKZ29_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37804.1"
                     /translation="MEDFKNYGSRDLEDKSNKQEDTLKVKKFLDTVNNKEIWKKNIAS
                     PSILGLVLEGVVNYQLLNEDDTPYLYGSDKGYDRVHFELANDSVSSIDIVKAFKLNMF
                     KLDPYGLYGLMSVLMESGIVGRGQAKALFMEQVYGVIQVMQIGERGERKWDALGLAMD
                     VEHAVKLFGESVLEDLEDVIDFIKSHPDKSREDIILRHLSRAGVKNL"
BASE COUNT          527 a          295 c          492 g          637 t
ORIGIN      
        1 gtcttaatgt tagccaagta tttttccaaa cgccacattg agatcgccaa tattagacat
       61 ataattgttt ccggcgttta cgcttttttt ccatttgtgg cgattatgct tcagccggat
      121 tttggttcgg ccctgataat ttttatgatt tggttaggga tgattttggt ttctggagtt
      181 tctaggaaac atttggcctg gttgggtttg gcggcaatca tcttttcagt cggggcgtgg
      241 atgtttgttt tgcaggatta ccaaaaagcg cggataatga cttttattca ccctttggcc
      301 gatgtgcgcg gttctggtta taacgcgttt caatcaacga ttgccgttgg ctccggccaa
      361 ttgacgggta agggggtcgg ttatggtacg cagtcaaggc tcaagttttt gccggaatac
      421 cagaccgact ttattttcgc tgcctttgct gaagaatggg gcttttttgg ggttcttatt
      481 ttcttctttt tgtttgggtt gattatctgg cgaattttag cgacggccat gaaaggggcg
      541 accaactttg aaattttgtt tggtttggga attgcggtga tgtttatgtc ccacttgata
      601 attcatgtcg gaatgaatat tggcattttg ccggtgaccg gcttgccatt gcctttttta
      661 agttatggcg gctcgcattt attagctgaa tttatgggct tgggtatttt gatggggatg
      721 aggcgttatt ccttggctta tcatcgggat gatgtgaaaa atgagtttat cggaccgcaa
      781 tagaaaatgc ggaatgaacg cagaactacg cagactagac gcggaactat gcagactgga
      841 caaaggtcaa cgtagacggg agctgataaa acgttgttta ctaggggtgg aaataattat
      901 tggttgttga tcaaaaaagt ccgaccccgc tagtcgctag cgaggtcgga ctttgaaaaa
      961 ctactagctc tcacgtgtga ggggttacgt gaggaagtta agttaacaaa tttgctaagg
     1021 aggacgattc tccccagact cttgttttta gccgttttta ggggtcggtt ttattttgtg
     1081 gataagttat ttgccagaag tggtcggggg gggtattatg agggtattag taatttatat
     1141 aaaatatatt ttatatggaa gattttaaaa actatggaag tagagattta gaagataaat
     1201 caaataagca ggaagatacc cttaaagtta aaaagttttt ggacacagtt aataataaag
     1261 aaatttggaa aaaaaatatt gcatctcctt ctattttggg tctcgtgcta gaaggagtag
     1321 taaattatca acttttgaat gaagatgaca ccccttatct ttatggtagc gacaaggggt
     1381 acgatagagt gcattttgaa cttgctaatg attctgttag cagtatagat attgttaaag
     1441 cttttaagct aaacatgttc aaactagatc cttatggact ttacggatta atgagtgttt
     1501 taatggaaag tggtattgta ggaaggggtc aggcaaaagc attatttatg gaacaggttt
     1561 atggggtgat acaagtgatg caaataggag aaagggggga aaggaaatgg gatgccctcg
     1621 gcctggcaat ggatgtagaa cacgcagtca agctttttgg agagtcagta cttgaagatc
     1681 tagaagatgt gatagatttt attaaatctc atcctgataa aagtcgggaa gacattatat
     1741 tacgacattt atcaagggcg ggcgtaaaaa atttataaca ctagtaaaaa gaaaaaggag
     1801 aggctgcgtc tctcctttct gaaatgtaga ttagtatcgc gttccgcgtt tgttccgcgt
     1861 agttctgcgt ctaatctgca tttgttctat ctttgataaa gttcaattgt ttcagcggcc
     1921 atctttttta gagaaaaatt ttctcggacg t
//
LOCUS       DAOSHV010000075         1930 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_59817_length_1930_cov_3.607467, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000075 DAOSHV010000000
VERSION     DAOSHV010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59817_length_1930_cov_3.607467"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="PKZ29_02680"
     CDS             complement(<1..327)
                     /locus_tag="PKZ29_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37805.1"
                     /translation="MKGGTKVKKHVLRLLALGLAIIFVIYAVPAVFAGNDKLPGEGSN
                     GQGHDWKEGTPGNSNANDNTQHNPNGYQNHLDCGCPTNTPKPTKTPKPTKTPKPTKTP
                     PPKTETP"
     gene            745..942
                     /locus_tag="PKZ29_02685"
     CDS             745..942
                     /locus_tag="PKZ29_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37806.1"
                     /translation="MASSPDTANQAPPLNADVFVKLEETSQQVDQIIEELNQSQQNRK
                     NRRHSDDRESKKDNRRQNKDD"
     gene            961..>1930
                     /locus_tag="PKZ29_02690"
     CDS             961..>1930
                     /locus_tag="PKZ29_02690"
                     /inference="COORDINATES: protein motif:HMM:NF014683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HOG37807.1"
                     /translation="MLFFALLPIFVFIVLVIFTKINLTKISLIFFGLTLILSLLVWQI
                     YPHIAIASTFKGALIAVDIFLIVFGALLFLKIIEGRQILDQIRLYLEKISPDYRIQTI
                     LIAWFFESFIEGVTGFGTPITIAAPILVSIGLSPITAVAVALLGNSTSVAFGAAGTPI
                     RVGFSGLNITGVPFYSALINLIGFIVPLFMLWLVTRRQPDGKNHFLDAIPFAVFSGFC
                     VVIPSFFTVFIGQEFSSIIGPIIGLILVIFAIKFKLFLPREIKTLRRSSPQTSLLPLK
                     TTLFPYTLLIILLVLSKLIFGNFGFNFNFGLSHNFSFFNPGLIFIIT"
BASE COUNT          435 a          487 c          307 g          701 t
ORIGIN      
        1 tggtgtctcg gtcttcggcg gtggtgtttt tgtcggtttt ggcgtttttg ttggctttgg
       61 tgtctttgtc ggttttggtg tatttgtcgg acatccacaa tctaaatgat tctggtagcc
      121 gtttggattg tgttgggtgt tgtcattggc attgctgttt cccggcgtcc cttccttcca
      181 atcgtgaccc tgaccattag acccttcacc cggaagtttg tcgttcccgg caaaaacagc
      241 aggaactgcg tagatcacaa agataatcgc caatcccaat gccagcagcc tcaaaacatg
      301 tttcttcacc ttagtccctc ctttcattat tgtctgtgtc caaaatctcc tttaaccatc
      361 ccagcactca cttatctcct ttcttatcca tattgttaaa ggacataaat accttttggt
      421 ctttgaacca aaatcaacgg attctatcac aaattcatca aaaaatcaat ttatcggtct
      481 cagccacaat ctccttcaca ggttaatcag cctgcttcca attatcgacc aactcttcct
      541 ccttccactg gcaacgtttc ttatcggcca tctccgactc tcactcccaa aactcccgtt
      601 atcgctccca caatccactc ttctcctgct tctcttccca cagcttactc tcagccaact
      661 gcttatattc ccccagatcc cactcttcct ccacctgtcc aacaaccata ttcccccgaa
      721 aatgttgtcg ataacagacc ggatttggct tcttctccgg atactgctaa tcaagctcct
      781 ccgcttaacg ccgatgtttt tgtcaagcta gaggaaacca gtcaacaggt tgatcagatt
      841 attgaggagt taaatcaatc gcaacagaac agaaaaaatc gccgccactc cgatgaccgt
      901 gaatccaaaa aagataatcg ccgccaaaat aaagacgatt aaattgtgtt agcataaatt
      961 atgctgtttt ttgctttact cccaattttt gtttttattg tcctagtaat ttttaccaaa
     1021 atcaatctca ccaaaatttc tctcattttt tttggcctca ccctgatctt aagtttgttg
     1081 gtttggcaaa tttaccctca tatcgctatt gcttccactt tcaagggagc ccttattgcc
     1141 gttgatattt ttctgatagt ttttggcgcc ctgctttttc tcaaaattat tgaaggtcgt
     1201 caaattttag accaaatccg tctttatctg gaaaaaatct ctcccgatta tcggattcaa
     1261 accattctta ttgcctggtt ttttgaaagt tttattgaag gtgttactgg ttttggcact
     1321 cctattacca ttgccgctcc tattttggtc agtattggtt tgtctccaat taccgctgtt
     1381 gccgttgctc tgttgggcaa tagcacttca gttgcctttg gtgctgccgg cactcctatc
     1441 agagtaggat tttccggtct aaatataact ggagtcccgt tttattccgc tcttattaat
     1501 ctcattggtt ttatcgttcc cttatttatg ttatggcttg tcacccggcg tcaacctgat
     1561 ggtaaaaatc attttcttga tgccattcct tttgccgttt tttccggatt ttgtgttgtc
     1621 attccctcat tttttaccgt ttttattggt caggagttct catcaattat tggtcccatc
     1681 atcggtctta tcttggtcat ttttgccatc aaattcaaat tatttttacc ccgagaaatc
     1741 aaaactcttc gccgctcgtc cccccaaacc agccttctac ctctcaaaac cactcttttt
     1801 ccctatactc ttttgattat tttattagtt ctcagtaaac tgatttttgg caattttggt
     1861 ttcaatttta attttggtct tagccataat tttagttttt ttaatcccgg tctgatcttt
     1921 ataattacct
//
LOCUS       DAOSHV010000076         1929 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_59858_length_1929_cov_3.575240, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000076 DAOSHV010000000
VERSION     DAOSHV010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59858_length_1929_cov_3.575240"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="PKZ29_02695"
     CDS             <1..327
                     /locus_tag="PKZ29_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37808.1"
                     /translation="SSQEFYQSASNFSAITRESSSVIRSINFNLSEHETTFDPKNPSY
                     IVSMLIDGNYSSILQTIKKITESRRLIDINSIQIKTPENNQSNTVVLNLSTGLFYLPV
                     YSNEKN"
     gene            314..634
                     /locus_tag="PKZ29_02700"
     CDS             314..634
                     /locus_tag="PKZ29_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37809.1"
                     /translation="MKKTKLSGYFVFISVFTFLAIFIVIVQRSYSNLMGPIKSVESNA
                     LLRPINPELDTGILEAIENRQEYIETGSMNIIPYTDNQSETGNVIPDLVVPPTPVATQ
                     SASN"
     gene            638..1897
                     /locus_tag="PKZ29_02705"
     CDS             638..1897
                     /locus_tag="PKZ29_02705"
                     /inference="COORDINATES: protein motif:HMM:NF039533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOG37810.1"
                     /translation="MKKEKRIPTILGLILILSTVFIGSRLVGRTTTSFTQASGECDPI
                     NPQITNLTHQTATVSFTTTGSCLVNVNIDNKTLPDTKDKGTIHYIEIVGLEESKAYLF
                     SVISGGKEFKSDSYNFKTAQKPNKDIPTSNLAWGRVLNPDQSPAGNAIVYLNIPGASA
                     LSAAVTSSGNWNIALATSFNESLTDWFTPPSNIEENITVIATDHPATQIINNTSHNNP
                     VPDIVLGQDKFSAPETLPDQTEDVILPDYTLDSSIPLNIINPTNNEQISSQKPDFFGT
                     GQPNSTLNIKIESPVTINGQTSVNQDGSWNWSPPQNLTPGEHTITVTDNNNKTITRKF
                     VVLAAENQTSFTASPSATIITPTPTPTIIQPTAVTTRPPAPTPIPVVRPSTSSGIPKT
                     GILFPNITLIILAVVSVVFATVYYKKS"
BASE COUNT          566 a          509 c          282 g          572 t
ORIGIN      
        1 tcatcccaag agttttatca atcggcttca aatttctctg ctatcacccg agaatcgtct
       61 tccgttatca gaagcataaa tttcaatctt agtgaacatg aaaccacttt tgaccctaaa
      121 aatccatcat atattgttag tatgttaatt gacggcaatt actcctctat cctccaaacc
      181 attaaaaaaa taaccgagag ccgtcgctta attgacatca attctatcca aattaaaact
      241 ccggaaaaca accaatctaa cacagttgtt ttaaatctct ctaccggatt attttattta
      301 cccgtctatt caaatgaaaa aaactaaatt atctggctat tttgttttta tttctgtttt
      361 tacttttttg gccattttta ttgttattgt tcaacgaagt tattcaaacc ttatgggtcc
      421 cattaagagt gttgaatcca acgctttgct tcgtcccatc aatcctgagt tagataccgg
      481 tattttggaa gccattgaaa accgacaaga gtatattgag accggcagta tgaatatcat
      541 tccttacact gataaccaat cagaaactgg caacgtcatt ccggatctcg ttgttccgcc
      601 gactccggtt gccacccaaa gcgcttcaaa ttaatttatg aaaaaggaaa aaagaattcc
      661 cactattctc gggttgatat tgattttaag cacagttttt attggctctc gcttagtcgg
      721 tcgcaccact acttctttta ctcaagccag cggcgaatgt gatcctataa accctcaaat
      781 taccaattta actcatcaaa ccgccaccgt ttcctttacc actactggat cttgtttagt
      841 caatgtcaat attgacaata aaactctccc tgataccaaa gacaaaggca ccattcatta
      901 tattgagatt gtcggtcttg aagaatcaaa agcctacctt ttttccgtca ttagtggggg
      961 caaagagttt aaatctgaca gttataattt caaaaccgct caaaaaccaa ataaggatat
     1021 tcccacttca aatctggcct ggggtcgggt attaaatcca gaccaatctc ccgccggcaa
     1081 tgctattgtt tatcttaata ttcctggcgc ctctgccctg tctgctgcgg ttacttcttc
     1141 tggcaactgg aatattgccc tggctaccag ttttaatgaa tccttaaccg actggtttac
     1201 cccccccagc aacattgaag aaaatatcac tgttattgcc accgaccatc ccgccaccca
     1261 aataatcaac aacacttccc acaacaaccc cgttcctgat attgtcttgg gtcaagacaa
     1321 attttccgcc ccagaaaccc ttcccgatca aacagaagat gttattctgc ctgattatac
     1381 cctggattca agcattcctc taaatatcat caatcccacc aacaacgaac agatatccag
     1441 ccaaaagcca gatttttttg gcaccggtca acctaattca accttaaaca tcaaaattga
     1501 atccccggtt acaattaatg gtcaaaccag cgtcaatcaa gatggcagtt ggaattggtc
     1561 cccgccccaa aacctaactc ccggcgagca cactattact gttactgaca ataacaacaa
     1621 gaccattacc cggaagtttg tcgttcttgc cgctgaaaat cagacctcat ttaccgcttc
     1681 tccctcagcc actattatta ctcccacccc tacacccacc atcattcagc ccaccgctgt
     1741 tactactaga ccaccagctc ctactccaat tccggttgtc aggcccagca cctcttctgg
     1801 cattcccaag accggaattc tttttcccaa cattacccta atcattttag ctgttgtatc
     1861 ggttgtcttt gccactgttt attacaaaaa gtcataaatc tgctaacata cattatggct
     1921 cgaacaagt
//
LOCUS       DAOSHV010000077         1907 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_60880_length_1907_cov_4.316955, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000077 DAOSHV010000000
VERSION     DAOSHV010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60880_length_1907_cov_4.316955"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="PKZ29_02710"
     CDS             <1..234
                     /locus_tag="PKZ29_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37811.1"
                     /translation="ATLTNFDGLGYISNVGSLKKVRGKGFGKIATLYCVAKSIKNNNQ
                     EHCLATEEGAYPNEFYKRIGFVTRFTACGYTKS"
     gene            complement(235..1116)
                     /locus_tag="PKZ29_02715"
     CDS             complement(235..1116)
                     /locus_tag="PKZ29_02715"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOG37812.1"
                     /translation="MTITKAVIAAAGRGTRFLPVVKAYPKELVPLISKPNIQYLIEEA
                     IGAGITEIAIVHRLGENSIEKYFSPDPELEKYLTETNKLDYLDSLKNIWSQTKLTFIP
                     QPTNLPYGTGSPLLAAKDFIGANPFVYMYGDDLTVEPKTGQFLTRMLQTFDQYHPDEI
                     IAVKDVGPEEISRYGSAQYMDDPNYPHRISAMLEKLPANQAPSTFAQGGRFVVTPKVF
                     DLLEHQSLSRDGELWFSDASNQLAKIGIVLTQAFEGAEDWLTTGDPLRWLIANITLAL
                     NDPNLKDSLTEFIKKLT"
     gene            1188..1871
                     /locus_tag="PKZ29_02720"
     CDS             1188..1871
                     /locus_tag="PKZ29_02720"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HOG37813.1"
                     /translation="MENELNLTKQGFISKSNSNKVVLILLVLILCLSVTILYRLTRTT
                     STISVTGRAEITAKPDRVSFIVTRVNTGVDASQAIDDGVAGINKLITLVREMGGSDVE
                     IKKTFNQVTPGTNGYTVANAFSVKTKRVNEVDYLIKKLYMGGASTVSNVSFESSDIAG
                     VEQRLRDDVYKDALEKAKRIARSAGKRLGGVVAITDDAGDNSSTVSTSMVENLISVSK
                     SITVVYKIW"
BASE COUNT          587 a          251 c          510 g          559 t
ORIGIN      
        1 gccactctga ctaattttga tggtcttggt tatatttcaa atgtagggtc gttaaaaaaa
       61 gtcagaggca aggggtttgg aaaaatagcg acactctatt gtgtggctaa gtcaattaag
      121 aataataatc aggaacactg tttggctact gaggaaggag cgtatccgaa tgagttttat
      181 aaaaggatag ggtttgttac acgatttact gcgtgtggat atacgaagag ttgattaggt
      241 taatttttta ataaattcag tcaaactgtc ttttaggttg gggtcgttga gggcgagggt
      301 gatattggca ataagccaac gaagggggtc gccggtggtc aaccaatctt cggcaccttc
      361 aaaggcttgg gtgaggacaa taccgatttt agcaagttga ttgctggcgt cggaaaacca
      421 gagttcaccg tcgcgggaga gagattggtg ttcgagcagg tcaaagactt tgggggtgac
      481 gacaaaccga ccgccttgag caaaggtgga gggagcttga ttggccggga gtttttccaa
      541 catagcggaa atacgatgag gatagtttgg atcatccata tattgagcgg agccataacg
      601 actgatttct tcaggaccaa catctttgac agcgataatt tcgtcggggt ggtattgatc
      661 aaaggtttgg agcatacgag tcaagaattg gccggttttt ggttcgacag taagatcgtc
      721 gccgtacata tagacaaatg ggttggcgcc gataaaatct ttggcggcta aaagagggga
      781 cccagtgcca taaggaagat tggtaggttg aggaataaag gtaagtttgg tttgagacca
      841 aatgtttttg agactgtcaa gataatcaag tttgttggtt tcggtaagat atttttctag
      901 ttctgggtcg ggagagaaat atttttcaat tgaattttca ccaagacgat gaacaatagc
      961 aatttcagta ataccagcac caatagcttc ttcaatcaga tattgaatat taggtttgga
     1021 gataagagga acaagttctt tgggataagc tttaactacg ggcagaaaac gagtgccgcg
     1081 accagcagcg gcaataacgg ctttagtgat agtcatagta agttatggta gcacataaat
     1141 gtagtggaag ttaattgatt taattttagg ttgtgattta attaattatg gaaaatgaac
     1201 taaatttaac taaacaaggt tttatttcta aatcaaatag taataaggtt gttttgattc
     1261 ttttggtttt aattttgtgt ttgagtgtga cgatactata tcggttaacg agaacaactt
     1321 cgacaatttc ggtgacaggt agggctgaga ttacagccaa acctgataga gttagtttta
     1381 tagttaccag agtaaatact ggcgttgatg cttcacaggc gattgatgat ggagtggcag
     1441 gaataaataa attgattact ttggtgaggg agatgggagg gagtgatgtg gagattaaaa
     1501 agacgtttaa tcaggttacg ccaggaacta acggttatac ggtagcaaat gcttttagtg
     1561 ttaaaacaaa aagagttaat gaggttgatt acttgataaa aaagttatac atgggaggag
     1621 caagtactgt ttctaatgtt agttttgagt ctagtgacat tgcgggggtg gaacaaaggt
     1681 tgagagatga tgtctataaa gatgctttgg aaaaggctaa acgaattgct aggtctgcag
     1741 gtaaacggtt gggtggggta gtggcaatta ctgatgatgc tggtgataat tccagtacgg
     1801 taagcacttc gatggttgaa aatttaatta gtgtatcaaa aagtatcaca gtagtttata
     1861 aaatttggta attgttgaga atgataatta accggaaaga gcaaggg
//
LOCUS       DAOSHV010000078         1895 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_61461_length_1895_cov_5.355435, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000078 DAOSHV010000000
VERSION     DAOSHV010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1895
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61461_length_1895_cov_5.355435"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="PKZ29_02725"
     CDS             complement(<1..587)
                     /locus_tag="PKZ29_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37814.1"
                     /translation="MKIQTSLKRIFISQTRLKLINIFFYKPEEIFYVRQLVRLADEEI
                     NSVRRELDNLQKSGLINSERRGNRLYYWADKQSPLFTNLVIIANQSSGLGLKLQNKNE
                     TLGTIKLVFYSYRFMVGGKRGSDDIDLIIVGDVSLREIEGFVKQEEAIRGHEINYMVM
                     GKGEFKLRRQKRDQFIVDFFLSDPVVIIGSCLEIC"
     gene            complement(591..1838)
                     /locus_tag="PKZ29_02730"
     CDS             complement(591..1838)
                     /locus_tag="PKZ29_02730"
                     /inference="COORDINATES: protein motif:HMM:NF022702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HOG37815.1"
                     /translation="MNPKVTKILGLLGIFLIVSGISYFLFDYFLPSGSGGLLKSFSQR
                     QPSSDQNQDQNPAKPELYPGPKTEACPLNGKLYTKEEKDTWSGRRPLMVMIENHQDSR
                     PQSGLQTADIVYEAVAEGGITRFMGVFYCGQVAGSEANKYDVGPVRSARTYFLDIASE
                     YSDYPLYNHVGGANCSAATPGGPCTTAKKAQAIEQISAYGWTNKGTWGDLSQFSLPYK
                     VCRREPDRTGQERATEHTMYCSTKELWNIASTRGLTNLTEAKKTSWDKNYRSWLFKQT
                     DQAAASPTATKVTFDFWSGYKDYTVVWNYDAANNNYVRTNGSVVQTDFNTDTSLTAKN
                     IIIQLVKESRSIDEHMHNLYDVVGSGSGVLIQNGEKTDITWSKTNRVSRTIYKDKSGK
                     EVNFIPGNIWVELLPTTSTVSYN"
BASE COUNT          549 a          374 c          408 g          564 t
ORIGIN      
        1 ttacaaattt ctaggcaact acctattata acaactgggt cgctaaggaa gaaatcaaca
       61 ataaactggt cgcgtttttg acggcggagt ttaaactcgc ccttgcccat gaccatataa
      121 tttatttcgt gaccacggat ggcttcttcc tgtttaacaa agccttcgat ttcacgcaga
      181 gaaacatcgc ccacaataat caaatcaata tcatcgctac ctcttttgcc accgaccata
      241 aatctataac tataaaaaac caatttgata gtgccaaggg tttcattttt gttttgcaac
      301 tttaacccca gacctgaaga ttggttggca ataatcacta aattagtaaa taaaggcgat
      361 tgcttatcag cccaatagta gagtctgttc ccccgtcgtt cagaattgat caagccgctt
      421 ttttgaagat tatcaagttc acgtctaact gaatttattt cttcgtcagc taaccgaacc
      481 agttgccgga cataaaaaat ctcctccggt ttgtaaaaga aaatattgat gagtttgagc
      541 cgggtttgag aaataaaaat gcgcttaaga ctggtttgaa ttttcatagg ctagttgtag
      601 ctgacagttg aagtggtagg aagtaattct acccaaatat tgccgggaat aaaattaact
      661 tctttgccgg atttatcttt gtaaatagtc cgagaaacgc ggtttgtttt ggaccaggtg
      721 atatctgttt tttcaccatt ttggataaga acgccgctac cagaaccgac gacatcgtaa
      781 aggttgtgca tatgttcatc aatagaccgg gattctttga ccagttgaat gataatattt
      841 ttggcagtaa gagaagtgtc agtattaaaa tctgtctgga caactgaacc gttggttcgg
      901 acataattgt tgttggcggc gtcataattc caaactacag tatagtcttt gtaaccagac
      961 caaaagtcaa aggtgacttt ggtagcagtg ggactggcag cagcttgatc ggtttgctta
     1021 aacaaccaag agcgataatt tttgtcccat gaagtttttt tggcttcagt aagattagtt
     1081 aaaccacgag tagaggcaat attccacaat tctttggtgg aacaatacat ggtgtgttca
     1141 gtggcccttt cttgaccagt gcgatcgggt tctcggcgac agactttgta gggcaaggaa
     1201 aattggctca gatcacccca agtgcctttg ttggtccagc cataagcgct aatttgctct
     1261 atagcttggg cttttttggc ggtagtgcag ggaccgccgg gagtagcagc actacaattg
     1321 gcgccaccaa catggttgta aagcggataa tcagaatatt cagaagcgat gtccaaaaag
     1381 taagttctgg cagagcggac aggaccaaca tcatacttgt tagcttcgga tccagcaacc
     1441 tgaccacaat agaaaactcc cataaatctg gtaattccgc cttcggcaac ggcttcgtag
     1501 acgatatcgg cagtttgaag cccagactga gggcgggaat cttggtgatt ttcgatcatt
     1561 accataaggg gacgccgacc agaccaagta tctttttcct ctttggtgta aagtttgccg
     1621 tttagaggac aggcttcagt ttttggaccg ggatataatt ctggttttgc cggattttga
     1681 tcctgatttt gatcactact tggttggcgc tgagagaatg attttaataa accaccgcta
     1741 cctgagggca aaaaatagtc aaataaaaaa taagaaatac cactgactat taggaatatg
     1801 cctagcagtc cgagaatttt ggtgactttg gggttcatta aaattttaat ctttaatagt
     1861 tttaactata gctaattttt cgtcttttga caaag
//
LOCUS       DAOSHV010000079         1846 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_64029_length_1846_cov_6.911781, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000079 DAOSHV010000000
VERSION     DAOSHV010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64029_length_1846_cov_6.911781"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="PKZ29_02735"
     CDS             <1..395
                     /locus_tag="PKZ29_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37816.1"
                     /translation="VGPTDSGKTWYINNILIPFFREKKMRVVYFSNPDNISENNKADI
                     IIVDEVEVLIDQDKLEASSSGHEPYYSEVYLNKVKAWHNKLQKLTVPSIFVLTRNNKK
                     EISNIVDNVVTTDWGAKVKCLAFNVSKS"
     gene            528..866
                     /locus_tag="PKZ29_02740"
     CDS             528..866
                     /locus_tag="PKZ29_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37817.1"
                     /translation="MGGGGGWGRLEIKYFHQLLIFLPFAYSKFFSAGFLNNKIKDGLI
                     LSSRLSEFAVEDERIEGSLDFARDDTFKFPKVARFNIQADFSKNKKSPRYIVIVWGTV
                     LPLNACFASV"
     gene            complement(841..1266)
                     /locus_tag="PKZ29_02745"
     CDS             complement(841..1266)
                     /locus_tag="PKZ29_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37818.1"
                     /translation="MTQRQQLNQKIVLRQLISQAIITPDTACPNCGHTHTEKEIRQGW
                     RNTPYDFTTKCVNCKRRFVAGLDVEGDSEELPLGHYQFLCPEQLYTAISDLMAERSRS
                     LLGTKFLLEKRPDLFWNIIRHHGNYERGLAAFRRWQNTH"
BASE COUNT          477 a          362 c          403 g          604 t
ORIGIN      
        1 tggttggccc aactgatagt ggcaagacct ggtatataaa taatattctc atcccgtttt
       61 ttcgggaaaa gaaaatgaga gttgtttatt tttctaatcc ggataatatt tcggaaaata
      121 ataaagcaga tattatcatt gttgatgagg ttgaggtatt aattgatcaa gataaattag
      181 aagccagctc gtcaggacat gagccgtatt attctgaagt atatttaaat aaagtgaagg
      241 catggcataa taaattacaa aaattaactg tgccaagcat ttttgttctg actagaaata
      301 ataagaaaga aattagtaat atcgttgata atgttgtaac aaccgattgg ggggctaagg
      361 ttaaatgttt ggcttttaat gtttcaaaaa gttaaaattt tttaatacaa taagttttcg
      421 aaaaattata taaataaatc tctattaata ctcgcccgtc ttattgtccg ccccttgtct
      481 gaaaaggcat cattcatatt ccccctcatt gtctgaacct gtccgccttg ggcggaggag
      541 ggggttgggg gaggttggaa ataaaatatt ttcatcagct tttaattttt ttgcccttcg
      601 cttattccaa atttttcagt gctggttttt taaataataa aattaaggac ggcttaattt
      661 tgtcatcccg actaagtgag tttgccgtgg aggacgaacg catagaggga tccctcgact
      721 tcgctcggga tgacaccttt aaatttccaa aggtggcaag gtttaatatt caagcggatt
      781 tttctaaaaa taaaaaatcc ccacgctaca tagttatagt gtggggaacg gtcttgccgc
      841 ttaatgcgtg ttttgccagc gtctgaaagc ggcgaggcct cgctcgtaat tgccatggtg
      901 cctgattatg ttccagaaca gatcaggccg cttctccaga agaaactttg tgcccagtag
      961 cgaacggctt cgttctgcca tcaaatcgct gatggccgtg tacagctgtt cagggcacag
     1021 gaattggtaa tgccccagag gcagttcttc cgagtcgcct tcgacatcga gcccggcgac
     1081 gaatcgtcgc ttgcaattca cgcatttggt agtgaaatcg tagggagtgt ttctccaccc
     1141 ttgacgaatt tccttttcag tatgggtatg cccgcagttt gggcaggcgg tatccggcgt
     1201 gattattgcc tgcgaaatca attgccgaag gacgatcttc tggttcagct gttggcgctg
     1261 agtcattgcc attatcatgt ccatgccctc cccttatctt gtttttctta gttcttcaaa
     1321 ggtgcgaaat tctgactaat tattgtagca agtcgggttt tgtctgtcaa gatgtgaaaa
     1381 aatggatatg gtctttcatt ttttgcttcc ttttctgctc atttgctttg ggtggaaaaa
     1441 tcggcgcaaa aaatcgccgc tagttggcgg atgtatagag atatctccat agatatgtat
     1501 agaaaaacct gtcccgagta ctcgggacag gttttgttga cttttgtgct aaaagtctcg
     1561 ttcggtaaaa tgcctaatag ggactttgag ttttagtctt tccaatacat ttcagcccat
     1621 tcttgtttct ccctttcttc ttcgcgggct ctttcttcgt cggttaattt taccttatca
     1681 tcttcgtaat tgcagtaatg tccactgctg aagggccctg tgggggcttt tcgccttttt
     1741 tttgaaagcc aatcaaaaac tcccattttc tattctcctc ttacttaaga tttggcaggt
     1801 accccgaaat tcggacaact attcctcaac tggtggtgat gggtta
//
LOCUS       DAOSHV010000080         1834 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_64647_length_1834_cov_6.234963, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000080 DAOSHV010000000
VERSION     DAOSHV010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64647_length_1834_cov_6.234963"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            394..1617
                     /locus_tag="PKZ29_02750"
     CDS             394..1617
                     /locus_tag="PKZ29_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37819.1"
                     /translation="MEKETVKSKHNHWGIKLLYNAFITVLFAVGTYHQLYVGDFNPHL
                     HYANYFIERGRSPVQHMVFHRLVIIVKALLPTNLISGLSYRFSEIVETHGWEIAGLAT
                     AILIYLATAWVLYFILKNSLKLDHTRFSEIKIWTLIVVCLFVMPINFFFLRERLILGY
                     FSANVLHNPTYILSRVFSLALFYLCLVWNEKEINLKTGVLLSVLSLLSTASKPNFMMA
                     FVPSLVIFFLIKNKSIKRLNYKLLFFVGIPAFIVLSTQFLMRSSSAETSKFVFAPFVA
                     VLTRVPNIPMVFLFLLLSLAFPISVFILNWKTVKQKASTQLLLITLTINFLLFILFAE
                     VPKTGHLNFIWGMMMAVFLSFVYAIIIWVNQSKLEDRQFWTIRSLLPFTFLLLHIASG
                     VLYYFSVVLYPGPVW"
BASE COUNT          490 a          370 c          349 g          625 t
ORIGIN      
        1 ttggggggca gccccctggg agagtaggtc attgccaaga gactttttca tttaaatact
       61 cgttaccaat ggctttcttt gtttctgatt gtgcctggga ttcccatact ctctgcattt
      121 tgccttccct gcggggaagg gggaccgctt gcggtggatg agggttcaat gaatccaagc
      181 tcacccgaat atttcattaa cgctatggga gcataattat ataaatcagc tatttaccgc
      241 cccatgataa aagacctgcg gctttagacc atatccccat tcgaaagcca tgcggtatca
      301 atggatttgt gtctcggttg cttaccgtta aacaaattga ttgaatttac tctcaaattg
      361 tcgatcattt agtttataat gctttattga gatatggaaa aagagactgt aaaatcaaag
      421 cataaccact ggggaataaa actcctgtac aacgcgttta tcacggttct gtttgctgtt
      481 ggaacctacc accagttgta tgtgggtgat tttaatcccc acctgcatta cgcgaactac
      541 tttatcgaaa gggggcgttc cccagtccag catatggttt ttcaccgcct ggttattatt
      601 gttaaagcgc tattgcctac caatttaatt tccggactaa gctatagatt cagcgagatt
      661 gttgaaactc acggttggga aattgctggt ttggcaaccg cgattctaat ttatctagcc
      721 acggcttggg ttctttactt tattctaaag aactctttaa aacttgatca cacccgcttt
      781 agcgagataa agatatggac attgatagtg gtttgcctct tcgtgatgcc gatcaacttt
      841 ttctttttgc gagaaaggtt gatattaggt tatttctccg caaatgtatt acataaccca
      901 acatatatcc tttcacgtgt tttttcttta gcgttgtttt atttatgcct tgtctggaac
      961 gaaaaagaaa taaacctcaa aacaggcgtg cttctttccg ttttgagttt attgtcaacg
     1021 gcttcaaaac caaactttat gatggctttt gtgccatcct tagttatatt ctttctgata
     1081 aaaaacaagt caataaagcg tttaaactac aaactacttt tctttgtcgg tattcctgct
     1141 tttattgtcc tctcaacaca gtttttaatg cgaagtagct cagcagaaac aagtaaattt
     1201 gtgtttgctc cttttgtagc tgttttgacc agagtgccca atatcccgat ggtttttctc
     1261 tttttactct tgtcattagc ttttccaatc agtgtgttca ttttgaattg gaaaaccgtc
     1321 aaacaaaaag caagcacgca attattgctc atcacgctaa caatcaattt tcttctgttt
     1381 attctattcg cggaagtgcc caagacgggt caccttaatt ttatctgggg aatgatgatg
     1441 gctgtcttcc taagcttcgt ttacgcaatt attatctggg tcaatcagag caaattagaa
     1501 gatagacaat tttggacgat cagatctctg ctgccattta cttttctgct ccttcatatt
     1561 gcgtcaggtg ttttgtacta cttttctgtg gttctttatc ctggtccggt gtggtgaaag
     1621 cgatttcatc tgattattga acaatgacgc tggaagtggt ctgttaatct ttcgtaaaat
     1681 actgtcactt aatcccgaaa aaccgataaa ctaaaactac catataaaat ctattgcgag
     1741 gcgattttgt gagtgatact cttttaacaa gatacaaaca aaaggagatg aaatgagaat
     1801 tattcgcagt ccaatattta ctctcaagcc ttat
//
LOCUS       DAOSHV010000081         1826 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_65123_length_1826_cov_6.454545, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000081 DAOSHV010000000
VERSION     DAOSHV010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65123_length_1826_cov_6.454545"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            65..703
                     /locus_tag="PKZ29_02755"
     CDS             65..703
                     /locus_tag="PKZ29_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37820.1"
                     /translation="MKRIIFLLLIVVFSGCATHAVIETPVVTKVYSKAFENKSIGYEI
                     LYSQPEPGILSEGKQMPLVPLEQAKLSVGSAKILRKLPDYIYQQLPSTVKCADHSNCD
                     YKLLVEMVAHNKRGPAYSDYEAGKSFAKGMATFGFASSEFDIIADFDVKYILLKEGKD
                     VFRKDYKVKESVDHQKGKFDSFNSLDEYTSQLFEKHLMLTLNNFFKEAEKVL"
     gene            1565..>1826
                     /locus_tag="PKZ29_02760"
     CDS             1565..>1826
                     /locus_tag="PKZ29_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006920140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37821.1"
                     /translation="MATRKWKKTNHAGLRYFDHESRKHGKKKDRYYAIRFRLDGQLHE
                     YGIGWLSDGIPEAIRKEEPNLGFEDYCLKLLRQYKANVKTGTG"
BASE COUNT          586 a          321 c          402 g          517 t
ORIGIN      
        1 atgaaaatga agattatcct acaaataaag cattgtatca tatcgaaaaa cataatgagg
       61 aaagatgaaa aggattatat ttctattgtt aatagttgtt tttagtggtt gcgcaacaca
      121 cgcggtcatt gaaaccccgg tcgttacaaa ggtttattca aaggcgtttg aaaacaaatc
      181 aatcggttat gagatccttt atagccaacc agagccggga attctcagcg aaggtaaaca
      241 aatgccgttg gttccacttg aacaagcaaa gctttcagtt ggatccgcca agatcctgcg
      301 gaagttgcca gattatattt atcaacaatt gccgtcaaca gtaaaatgcg cggatcattc
      361 taattgtgat tataagttgc tcgttgaaat ggtagcccac aataaaaggg ggccagcata
      421 ctcggattac gaagctggta aaagctttgc taagggcatg gcaacattcg gattcgcttc
      481 atccgaattc gatatcattg ctgattttga tgtaaagtat attcttctta aagagggcaa
      541 ggatgttttt aggaaagact acaaggttaa agaaagcgtc gatcatcaaa agggaaaatt
      601 cgacagtttt aattcactgg atgaatatac ttcacagtta ttcgaaaaac atcttatgct
      661 gacattaaat aatttcttta aagaagcgga aaaagttctt taagcaaaac aaccacgaaa
      721 gcggctggca attagctttc ttaatttgcc agccgttaag gttgagggtt tggcctcagg
      781 tatcatgtat gttccccctt tggtattttg aaaggtggtt tgacggtgct attttattta
      841 caaggcttga ttttttcatt ctgaccgagt cggtgttttc atacaaaaga catgggatca
      901 tatttgggtg attagtacag tttgtcaaat ctaacggagt agttccagtc ctacatttaa
      961 aagtctaggg acaggtcttg aaatgacagt tttattaagc tccgccattc cagcctgttg
     1021 cattatgcgt cgttgaagat ttgaggtagt aatcctcacc tcaatttaaa atctaaacgt
     1081 gttgactttt aggcgaaaag gaaaaggacg aatcaaccgg atgaaactca aattaaacat
     1141 atcttgccat cctgtgccct accctgccga tcaatgaata aaaggctaaa gagattccca
     1201 atgggaataa tgaaccgtcg gaagaatgcc cggaacgtta tttgggttac atggttctta
     1261 tccaattttt tttgtccaaa agtggggatt ttttaaagtt aagaaaaaag cacgttggca
     1321 ttaattgtaa gtaattgatt ttatttggct ggggaacaag gattcgaacc ttgattaacg
     1381 gagtcagagt ccgatttcat atctttcacg aactttctcg aacctataaa ataccattga
     1441 caaataaata acttatcgct atattaacca cgaatgaaac ttcatgaaac atcgtttttc
     1501 aggggaaata gtgggaatat agtgggaaca aatttaaaag gccaattaac gaagggggtt
     1561 aatcatggca acgaggaaat ggaagaaaac gaaccacgcg gggcttaggt atttcgatca
     1621 tgaaagccgg aagcatggca agaagaagga ccgttattat gcgatccggt ttcgtcttga
     1681 tgggcaattg catgaatacg ggattggctg gttatccgac gggatacccg aagccatacg
     1741 caaggaagaa ccgaacctgg gctttgaaga ttactgttta aagcttcttc ggcaatacaa
     1801 ggcaaacgtc aagaccggca ccgggc
//
LOCUS       DAOSHV010000082         1748 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_69513_length_1748_cov_4.227998, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000082 DAOSHV010000000
VERSION     DAOSHV010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69513_length_1748_cov_4.227998"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="PKZ29_02765"
     CDS             complement(<1..675)
                     /locus_tag="PKZ29_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37822.1"
                     /translation="MKRNRKNYLGLAVLLGLGVMVAAAWGQTREETGRDIKLGVIADE
                     GLGLVSISKDRQMISQMKIMGENEVWIPGGLGWYRAEVVKKILSQEKEPEWGRNVFIY
                     GLGFYPDKVIWTEKIDDWKSRYWWQMLKNNNLLNKNENLSGGIDENEIYLDQVMLRDF
                     AETKIVNEDIKLSVINISQVNGLAGFVTKRLERLGFSVISMETEESEKAGDCRILYGS
                     GAGESYA"
     gene            complement(662..1330)
                     /locus_tag="PKZ29_02770"
     CDS             complement(662..1330)
                     /locus_tag="PKZ29_02770"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HOG37823.1"
                     /translation="MTEKIKIIYQNKDYLVADKPAGITTTKEKKGETGTLEDFLAITY
                     PNNLPRNGIVHRLDKGTSGLMLAAKNQKTWLFLKKQFKERLVVKKYYCLVGGEASVDG
                     SINWPIGRSKHRFGKFEVRVEGKAALTEFKRIKTYVKEGKKYGLLEVNLKTGRTHQIR
                     VHMSHLRWPLVGDRVYGGETEILKRPFLQAFWLEFNNGMKRVCYTTELADDLKDHLKL
                     YEEK"
     gene            complement(1296..1700)
                     /locus_tag="PKZ29_02775"
     CDS             complement(1296..1700)
                     /locus_tag="PKZ29_02775"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF013421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HOG37824.1"
                     /translation="MLNVKRKKKLDLGWVGLMAVLSVIHNRLANSLEVVANRGISFGW
                     QVPVVEIILVLVLGYWWWRKGQLGWADKSISLILAGGGVNLIDRLRFGYVRDYWIWKW
                     FYNNIADWVIGLGVVWMLIKFNDREDQNNLSK"
BASE COUNT          483 a          542 c          161 g          562 t
ORIGIN      
        1 agcataactt tcccctgccc cgctgccata taatattctg caatcacccg ccttttccga
       61 ctcctccgtt tccatcgaaa ttaccgaaaa acccaatctt tccaatcttt ttgttacaaa
      121 ccctgccaaa ccattaacct gactgatatt aattactgat aattttatat cctcgttgac
      181 tattttagtc tccgcaaaat ctcgtagcat cacctgatcc aaataaatct cattttcatc
      241 tattcctccc gataagtttt catttttatt caacaaatta ttatttttta acatctgcca
      301 ccaataacga ctcttccaat catcaatttt ttccgtccat attaccttgt caggataaaa
      361 acccaaacca taaataaaaa catttctccc ccattccggt tccttttcct gcgacaatat
      421 tttttttacc acttccgccc gataccaccc caaccctccc ggtatccaca cctcattttc
      481 tcccattatt ttcatctgac tgatcatctg cctatcttta gaaattgaca ccaaccccaa
      541 accctcatca gctatcactc ccagttttat atctcttccc gtctcttccc tcgtttgccc
      601 ccatgccgcc gccaccatca cccctaatcc caacaatacc gccaatccca aatagttttt
      661 tctatttctc ttcatacagt ttcaagtggt cctttaaatc atctgctaac tctgttgtat
      721 agcaaaccct tttcattccg ttattaaatt ccaaccaaaa agcctgcaaa aatggccgtt
      781 tcaatatctc tgtctccccg ccataaaccc tgtcgcccac caacggccac cttaaatgac
      841 tcatatgcac cctgatttga tgagtcctgc ccgtctttaa attaacttcc aacagaccat
      901 acttctttcc ttctttaaca tatgttttaa tccgtttaaa ttctgtcaac gccgccttgc
      961 cttctaccct cacctcaaat tttccaaatc tatgttttga ccttccaata ggccaattta
     1021 tactaccatc aaccgacgct tcacccccca ccaaacaata atacttcttg accaccaatc
     1081 tttctttaaa ttgttttttc aaaaataacc aagtcttttg gtttttcgcc gccaacatca
     1141 gtcccgatgt ccccttatcc aaccgatgca caatcccatt tctgggcaaa ttattaggat
     1201 acgttattgc caaaaaatcc tccagcgtcc cggtttctcc cttcttttct ttagtcgtag
     1261 ttattcctgc cggcttgtcc gccactaaat aatccttatt ttgataaatt attttgatct
     1321 tctctgtcat taaattttat taacatccaa accactccca atccaattac ccagtcggca
     1381 atattattat aaaaccactt ccatatccaa taatccctta catatccaaa ccttaaccta
     1441 tcaattaaat taaccccgcc accagccaaa attaagctaa tacttttatc tgcccaaccc
     1501 aattgaccct ttctccacca ccaatatccc aacaccaata ccaaaattat ttccacaacc
     1561 ggcacctgcc agccaaaact gattcctcga tttgccacca cctccaaact attggccaac
     1621 cggttatgaa ttactgacaa cactgccatc aaccctaccc aacctaaatc aagcttcttt
     1681 tttcttttga cattcaacac aagtagttgc ttccggatga atggctaatc tctcagtatc
     1741 aatcattt
//
LOCUS       DAOSHV010000083         1659 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_75267_length_1659_cov_5.176434, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000083 DAOSHV010000000
VERSION     DAOSHV010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75267_length_1659_cov_5.176434"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            182..808
                     /locus_tag="PKZ29_02780"
     CDS             182..808
                     /locus_tag="PKZ29_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37825.1"
                     /translation="MIKLLIEYSKEFELERIESTINRLDWYLSNKYSLSSLSFPKNID
                     VDNLKEIKKEELVEAVGEEYDESKFVTTAESIVQMYEKYEVKLEDFIKSIGLSVIPEV
                     KVSLTNYGIGGSYHLPNEIIVNINRFFSIGLIRNILHEIIHLHIQALIDQYKISQWNK
                     ETIVNLLFEKAFPEIYKEPNIPMDTTKIRKIFEENFPNIKKVISLISE"
     gene            856..1200
                     /locus_tag="PKZ29_02785"
     CDS             856..1200
                     /locus_tag="PKZ29_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37826.1"
                     /translation="MSKTYKFLPEDFLELRKKIIELEQEVIEFGREVGESTEQSSETW
                     HDNATYDVARFNFETTAAKLQELIEIRNKATLIEPRTQPPDKVVIGSTVKILNLDTGQ
                     TQLVKITRDTVK"
     gene            1383..>1659
                     /locus_tag="PKZ29_02790"
     CDS             1383..>1659
                     /locus_tag="PKZ29_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37827.1"
                     /translation="MFWVKFTWILTYFIYFPITRICQHVKTTIDNSFFDYDFRPDRPL
                     AIIANHKTIFDPWLISSVIPFQTFLKILPIRIMGSVTFVDRIAHFFDK"
BASE COUNT          541 a          329 c          327 g          462 t
ORIGIN      
        1 gcgggttaaa ggcaaaggcg taccaagcga acgcggccgc cgtggcgatc tcttggttcg
       61 agttactttc ccgaccccag gcaaactctc ccgcaaagcc aaaaaaatca ttgaggacct
      121 ccgagaagaa gggctataga attggggtgt gttatttaag ataaataatg aaataaccca
      181 aatgattaag cttttaatag aatactcaaa agaatttgaa ttagaaagaa tcgagagtac
      241 tatcaacaga cttgattggt atttgagcaa caaatatagt ttaagctctt tatccttccc
      301 caaaaacatt gacgttgata atcttaaaga aataaaaaag gaggaattgg ttgaagccgt
      361 tggggaggaa tacgacgaaa gtaagtttgt cactactgcc gagtcgattg tacaaatgta
      421 tgaaaaatat gaggtcaagc tagaagactt tattaaaagt attggtctgt cggtaatacc
      481 agaagttaag gtctctttaa ctaattatgg tattgggggt agttatcatt tacctaacga
      541 gataatagta aatataaatc gttttttcag tatcggattg ataagaaata tcctgcatga
      601 gataattcat cttcacatcc aagccttaat tgatcaatat aaaataagtc agtggaataa
      661 agaaacaatt gttaacctcc tctttgaaaa ggcgtttcct gaaatttaca aggaacctaa
      721 catcccaatg gatactacta aaatcagaaa gatatttgaa gaaaattttc ctaatattaa
      781 gaaggttatt tctttaatta gcgaatgaac atattggtct cggtgtaaag ttttatatga
      841 gtttaaaata attttatgag caaaacttac aaatttttgc ctgaggattt tttagagtta
      901 cggaaaaaaa ttatcgaact ggaacaagag gtcattgaat ttgggcgcga ggtgggggaa
      961 tctaccgagc aaagctctga aacctggcac gacaacgcca cctacgacgt ggcccgtttc
     1021 aactttgaga cgactgccgc caaactgcaa gaattgattg aaattcggaa caaggctaca
     1081 ctcatagaac caaggacaca accgcctgac aaggtcgtca ttggtagcac ggtaaaaatt
     1141 ctaaacctgg ataccggcca aacccaactg gtcaaaatta cgagggacac ggtaaaataa
     1201 tcggttgatt tcaatcctct tcttttagca ttatttttgt gatatagttt aagtacggga
     1261 aaccgcgctt ttagtttatg aagacaagaa aggcccttgc gagccaatat accgcaagcc
     1321 cagtcgctca agctcattcg cgcgcttacg tcgcgccacg agccaaaata gcccccaggg
     1381 gcttgttttg ggtcaagttc acctggattc tgacttattt tatttacttt cccattaccc
     1441 gcatctgcca acacgtcaag acaacaatag acaattcctt ctttgattac gacttccgac
     1501 ccgaccgccc gctcgctatt atcgccaacc acaagacaat ctttgatccc tggttaatca
     1561 gtagcgtcat tccgtttcaa acttttctca aaattttacc aattagaata atgggctccg
     1621 tcacttttgt tgaccggatc gcccattttt ttgataaga
//
LOCUS       DAOSHV010000084         1656 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_75493_length_1656_cov_4.272954, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000084 DAOSHV010000000
VERSION     DAOSHV010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75493_length_1656_cov_4.272954"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="PKZ29_02795"
     CDS             <1..224
                     /locus_tag="PKZ29_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37828.1"
                     /translation="LFGKSITASGESASQLVINGSIYGLNSVVLNRSFNPKKSNNTTP
                     AVEVRYNPSLIFKLPAKMIQNITRWRLLQ"
     gene            254..997
                     /locus_tag="PKZ29_02800"
     CDS             254..997
                     /locus_tag="PKZ29_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37829.1"
                     /translation="MNKKTKNIKQQGQVALMVILASALILTLGLSASRQATVETKIDT
                     DQEMLKKAFNAAESGIEHYFGTGSTKYTGDELQGSANLNVKNFGDSQKLSFNSVTLPG
                     DTEYFWLVDRQINGSLGSNYYSTSNPSISICDLDKKTGTYKISYFYLSGSTYTRSNQV
                     VTTDSTGCIDGGFNLLAGNSLLLTVTPIGWASKIQLGGSTNFPIQGEEISSTGMVSGV
                     NNTVTILNRYEVPGFMLEAITSGGKVTNN"
     gene            1011..>1656
                     /gene="pilM"
                     /locus_tag="PKZ29_02805"
     CDS             1011..>1656
                     /gene="pilM"
                     /locus_tag="PKZ29_02805"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HOG37830.1"
                     /translation="MSNVVGLDIGQNGIKAVSIDKDVAGKLSLDLIGEVKSPQTDSSS
                     DGSKNKYFEALSQSLKTLISDLKIKGKQVVVNLPENEVVSRLVRLPPLKDSEIIDALR
                     FEAETFVPYPLEKVSIDYEVVEKDDAGRITVFVIAAKNDLINEYIKIFKSLGMELIAI
                     ESSSVAYRRMIRSGVISVERVVVLDIGEKYSDILNINKGNVYFSRSLPVGGESIT"
BASE COUNT          540 a          248 c          362 g          506 t
ORIGIN      
        1 tcctttttgg taagagtatc actgccagtg gtgaatcagc ctctcagcta gttatcaatg
       61 gttctattta tggtttaaac agtgttgtct taaaccgaag ttttaatcct aagaagtcca
      121 acaacacaac tccagcagtt gaagtcagat ataacccctc attaatcttt aaattacctg
      181 ccaagatgat tcaaaatatt actaggtgga gattactgca gtgaaaagtt tattaagtta
      241 taaagtagaa agaatgaata aaaaaacaaa aaatataaaa caacaaggac aagtagcgtt
      301 gatggtgata ttggcttcgg ctttgatttt gactttggga ttgtcagctt cccgtcaggc
      361 cacagtggaa actaaaatcg ataccgatca agaaatgtta aaaaaagctt ttaatgcggc
      421 tgaatctgga atcgaacatt actttggcac tggcagtacc aaatacactg gagatgaact
      481 ccaaggttca gccaatttaa atgttaaaaa ctttggtgac agtcaaaaac tatcattcaa
      541 cagtgtgact ctaccaggag atacagaata cttttggtta gttgacagac agataaacgg
      601 atctttggga agcaactatt acagtactag taatccttca atttcaattt gtgatctgga
      661 caagaaaacc ggtacttaca aaatcagtta cttttaccta tcaggttcaa cctatactag
      721 atcaaatcaa gtagtgacaa ctgattctac tggttgtatt gatggtggtt ttaatctgtt
      781 agccggtaat tctttgttac taactgtaac tcccatcggt tgggcttcaa agattcaact
      841 aggtggctca accaacttcc caattcaagg tgaagaaatc agttcgacag gaatggtctc
      901 aggagttaat aatactgtca ccatcttaaa tcgttatgag gtaccaggtt ttatgctgga
      961 agcaataact tcaggtggaa aggtgacgaa taactagtta tactggatgt atgagtaatg
     1021 ttgtaggttt agatattggt caaaacggga ttaaggcggt ttcaattgac aaggatgttg
     1081 ctggaaaatt gtcattagat ttgattgggg aggttaaatc acctcaaacg gatagcagta
     1141 gtgacggttc taagaataag tattttgaag cgttgtcaca gtctttaaaa acattaattt
     1201 cggatttgaa aataaaaggt aaacaggtgg tggttaattt acctgaaaat gaggttgtga
     1261 gtcgtttggt tagactgcca ccgcttaaag attcagagat tatagatgct ttgaggtttg
     1321 aagcggaaac atttgtcccc taccctcttg agaaagtatc aattgattat gaggtggtag
     1381 aaaaagatga tgctggcaga attacagttt ttgtgattgc ggctaaaaat gatttgatta
     1441 atgaatatat aaaaatattt aagtcattgg ggatggagct gatagcaata gaatcatcgt
     1501 cggtggcgta tagacgaatg atccgttctg gggtaatttc tgttgagaga gtggtggttt
     1561 tggatatagg tgaaaaatat tctgatattt tgaatataaa caaaggaaat gtttattttt
     1621 cgagatcatt gccggtgggg ggtgaatcga ttaccc
//
LOCUS       DAOSHV010000085         1639 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_76707_length_1639_cov_4.834596, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000085 DAOSHV010000000
VERSION     DAOSHV010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76707_length_1639_cov_4.834596"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            62..250
                     /locus_tag="PKZ29_02810"
     CDS             62..250
                     /locus_tag="PKZ29_02810"
                     /inference="COORDINATES: protein motif:HMM:NF015947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HOG37831.1"
                     /translation="MAKIRKVIKTGNSLAITIPSKSIKDFNIREGDPVVVKTNRTKVS
                     LTYTFSGHPRQLSLIGHK"
     gene            253..1623
                     /gene="secD"
                     /locus_tag="PKZ29_02815"
     CDS             253..1623
                     /gene="secD"
                     /locus_tag="PKZ29_02815"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HOG37832.1"
                     /translation="MHSRKKTLRIFWSILALTLFCFLINFPKQLPLKIHLGPINWEHT
                     FYRPQLNLKLGNNILKNNFDLKYGLDLAGGSSLIFDIDTSNRNSQELTQALESLKTNI
                     ERRINLFGVSETNVRIDKIQSQHRLQVDLPGIENTADAIDLIGTTAQLGFFGVINDQQ
                     FTQATSSAEFLSSIKDTGLNGSHLKNSSVQINPNNSKPEVSLEFNDEGSKLFAQATKD
                     YLNQKIVIFLDNRILTAPTVQTVITEGKASISGDFTSQSAKELSIQLNAGSLPLPLKL
                     VQQSQIGATLGQNSLNKGIFAGIVGLIVVALFMVANYGKLGLIANISLIIYSLITLTL
                     YRLIPVTLTFPGIVGFILSVGMAVDSNILIFERIKEESRRGKDWNMAMETAFGRAWDS
                     IKDANTCTIITGFILFNPFNWSFLSTSGMIRGFAVTLILGIFVSIFTGVYVTRNLLRV
                     FAKEKK"
BASE COUNT          464 a          368 c          270 g          537 t
ORIGIN      
        1 atttcttcct tagcgaccca gttgttataa taggtagttg cctagaaatt tgtaaccttt
       61 aatggccaaa atcagaaaag taattaaaac cggcaatagc ttagctatta ccatcccttc
      121 aaaatcaatc aaggatttta atattagaga aggtgatccg gttgtggtca aaactaaccg
      181 caccaaagtt tctctcactt atactttttc cggtcatccc cgccaattat ctctaattgg
      241 tcataaataa atatgcattc gcgtaaaaaa accctcagaa ttttttggag cattttagct
      301 ctgactttgt tttgttttct gattaatttt cccaaacaac tgcccctaaa aattcactta
      361 ggccccatca attgggaaca cactttttac cggcctcaac taaacttaaa acttggtaac
      421 aatattttaa aaaataattt tgatctaaaa tatggtcttg atttggcggg tggttcctcc
      481 cttatttttg atattgacac ctccaaccgc aattctcagg aattaactca agcccttgaa
      541 tctctcaaaa ctaatatcga acgaagaatt aacttatttg gcgttagtga aaccaacgtt
      601 cgtattgaca aaattcaatc ccaacaccgt cttcaggttg atttgcccgg tatcgaaaat
      661 acagccgatg ctattgactt aattggcacc actgcccagc ttggtttttt tggggttatc
      721 aatgaccagc aatttaccca ggccacctct tctgctgagt ttctctcatc catcaaagac
      781 actggtctca acggttctca tctcaaaaat tcttctgtcc agataaaccc caacaactcc
      841 aaacccgaag tctctttgga attcaatgat gaaggctcca aactttttgc tcaagctacc
      901 aaagattatc ttaaccaaaa aattgttatt tttctagata accgtatttt aaccgcacct
      961 acagtccaaa ctgttattac cgaaggcaaa gcctcaatca gtggcgactt tacttctcaa
     1021 tcagccaaag agctgtcaat tcaacttaat gccggatccc tgcctttacc tctaaaatta
     1081 gttcagcaat ctcaaattgg cgccactcta ggtcaaaatt cgctcaacaa aggcattttt
     1141 gccggtattg tcggcttgat tgttgtagct ttgttcatgg ttgccaatta tggcaaactc
     1201 ggcttaattg ccaacatcag tcttatcatt tacagtttga tcactcttac tctttaccgt
     1261 cttatcccgg taactctaac ctttcctggt attgttggct ttattttatc tgttggtatg
     1321 gctgtggata gtaatatttt gatatttgag cggatcaaag aagaatcgcg ccgcggcaaa
     1381 gattggaata tggccatgga aaccgctttt ggtcgcgcct gggattctat caaagatgct
     1441 aatacctgca ctatcatcac cggctttatt ttatttaacc cttttaattg gtcgtttttg
     1501 agcacttccg gcatgatccg cggttttgct gtcactctca ttttgggaat ttttgtcagt
     1561 atttttaccg gcgtttacgt taccagaaac ttattacgtg tttttgccaa ggagaaaaaa
     1621 taatatgaac gtcatgaaa
//
LOCUS       DAOSHV010000086         1623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_77839_length_1623_cov_4.957270, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000086 DAOSHV010000000
VERSION     DAOSHV010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77839_length_1623_cov_4.957270"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..754)
                     /locus_tag="PKZ29_02820"
     CDS             complement(8..754)
                     /locus_tag="PKZ29_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37833.1"
                     /translation="MGGKNKTKWIIRTVGEVSYEVSEGDRVVAGKLLARVKPKVVESF
                     NFAQFFGKLDGEKLEQLNQSFKNSWVNSGDLLCLTGGLFPKKICFPMSGSFLEIDEFG
                     NLKIERIEEEEKEIKSPVKSKVSKIEPEKIVLDFEAVELGGEGIVEGKAWGSGEIKVI
                     DDIRELKSCLRGEVLFTHNVSRPFLLKAEVVGITAVVTEGEIKPGEVDLGLPVLKLTG
                     EAWKEAMKFKGKKVNVLVNSRVGRLLIVLE"
     gene            complement(821..1072)
                     /gene="rpsR"
                     /locus_tag="PKZ29_02825"
     CDS             complement(821..1072)
                     /gene="rpsR"
                     /locus_tag="PKZ29_02825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOG37834.1"
                     /translation="MMKKSRKDSLLIARRKGKEKGCSFCKNKTVPMWQDYEKYYEYLS
                     PRSRIVARQYSGVCARHQRKLVKVIKQARHLGLLPFTTK"
     gene            complement(1079..1615)
                     /gene="ssb"
                     /locus_tag="PKZ29_02830"
     CDS             complement(1079..1615)
                     /gene="ssb"
                     /locus_tag="PKZ29_02830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOG37835.1"
                     /translation="MPSRCLNKVTLIGNLTRDPELRYTPGGMAVVSFGLATNRVWVTK
                     QGERKEDAQFHRIVAWNKLAELCSQLLSKGRRIYVEGRIQYREFTDAENNKKQIAEII
                     IDDMIILDNKGVGGADHGEAIDLAGTPTEENETPAAEGGIEDIVIPDDIGEVATEEKP
                     EVEKTEDTADKKDEEMPF"
BASE COUNT          406 a          462 c          207 g          548 t
ORIGIN      
        1 catgttttta ttctaacact attaacaatc ttcccaccct ggaattaacc aacacattta
       61 cttttttccc tttaaatttc attgcttcct tccaggcttc accggtcaac ttgagcaccg
      121 gcaaacccaa atcaacttct ccgggtttaa tttccccctc cgtcaccacc gctgttattc
      181 ccaccacttc cgcctttagc aaaaatggcc ggcttacatt atgggtaaac aacacctcgc
      241 ctctcaagca tgactttaac tctctgatat catcaattac ttttatctct ccacttcccc
      301 aagccttgcc ttcaacgatt ccttctcccc ccaattccac cgcctcaaaa tccagcacaa
      361 ttttctccgg ttcaattttt gataccttac ttttcaccgg tgatttaatt tccttttctt
      421 cctcctctat tctttctatt ttaagattgc caaattcatc tatttccaaa aaacttccgc
      481 tcatcggaaa acatattttt ttaggaaaca acccccccgt caaacacaac aaatctccac
      541 tgttaaccca gctgttttta aaactttggt ttagctgctc caatttttct ccatccaatt
      601 tgccaaaaaa ctgggcaaaa ttaaatgatt ccaccacttt aggtttgact ctagccagta
      661 attttcctgc taccacccta tccccctcac ttacctcata cgacacttcc cccaccgttc
      721 ttattatcca ctttgtttta tttttaccgc ccatgattat taattataac caaaaatccc
      781 cctgatacca aaagtaatca gggggatttt aaaaggcttc ttatttagtc gtaaaaggca
      841 gcaatcccaa atgtctcgcc tgcttaatca ccttcaccaa cttccgttga tgtctggcgc
      901 agactccact atattgacga gccacaatcc ggcttcgggg agacaaatac tcatagtatt
      961 tctcatagtc ctgccacatc ggcacggttt tgttcttaca aaaagaacac cccttttctt
     1021 ttccttttct tctggctatc aataaactgt cttttctgct ttttttcatc ataagttatt
     1081 aaaatggcat ttcttcgtct tttttatcgg cagtgtcctc agttttttcc acttccggtt
     1141 tttcttcagt cgccacttca ccgatatcat ccgggatgac gatgtcttca attccccctt
     1201 cagctgccgg agtttcattt tcttctgtcg gagttcccgc caaatcaatg gcttctccat
     1261 gatcagctcc gccgactcct ttgttgtcca aaataatcat gtcatcaata ataatctcgg
     1321 caatttgttt tttattgttc tcggcatcgg taaattcccg atattgaatt ctgccctcaa
     1381 cataaattct ccttccctta gacaataatt gagaacaaag ctcggctagt ttattccaag
     1441 ccacaattcg gtgaaattga gcatcctctt ttctttcacc ctgtttagtt acccaaactc
     1501 ggttggtagc tagtccaaaa gacactaccg ccattccccc cggtgtatat ctcaactccg
     1561 gatcacgggt taaattacca attaaagtta ccttgttcaa acacctgcta ggcatatgtt
     1621 tta
//
LOCUS       DAOSHV010000087         1553 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1718 NODE_83335_length_1553_cov_6.693591, whole genome
            shotgun sequence.
ACCESSION   DAOSHV010000087 DAOSHV010000000
VERSION     DAOSHV010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863247
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:16:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 569
            CDSs (total)                      :: 555
            Genes (coding)                    :: 554
            CDSs (with protein)               :: 554
            Genes (RNA)                       :: 14
            rRNAs                             :: 1, 2 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 11
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83335_length_1553_cov_6.693591"
                     /isolate="AMR_MDS_1718"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            80..271
                     /locus_tag="PKZ29_02835"
     CDS             80..271
                     /locus_tag="PKZ29_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013910043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HOG37836.1"
                     /translation="MTKILLDQDKCIGCNTCPLIDSEVFEMDTTIYKAKVKNQPGKVT
                     ESVQSAIDSCPVGAISAEE"
     gene            289..1311
                     /locus_tag="PKZ29_02840"
     CDS             289..1311
                     /locus_tag="PKZ29_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG37837.1"
                     /translation="MTKKIKKNLKPKSEPVATKSVTIPLVGVIIFSLLLAVSSFFAGA
                     AWLKTNGGAVAVKAKNVFEAKKSDKPEFQFYVMSFCPFGNDAEDVIKPVFDLLGKSAD
                     IRPQYIFSKVEGSLNDYCQKNVPDPANCQMYVQNSQGQLKSVEDCKAQINTMLKECTN
                     ESQYLKIGNNYYGSLHGRIEANQDVREICAYNQSEDKTNWWKFVENVNKNCTDKNADT
                     CWEDQAKQAGLDTTKITECFNNQAGELIEKEIALTTKNSVQGSPTLFVNGIAFPPESA
                     YTQDNTGTLKIGNKVFTQSQYRSPDAIKEAICASFNRPPKECKTTLETPKSPDGQVQG
                     ATEGGC"
     gene            1372..>1553
                     /locus_tag="PKZ29_02845"
     CDS             1372..>1553
                     /locus_tag="PKZ29_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HOG37838.1"
                     /translation="MKKLNSHQIRRSRPSTAQKMQSQIKRHPLVLVLDHVLDTYNIGS
                     FFRLADALGVQKLYLCG"
BASE COUNT          489 a          333 c          286 g          445 t
ORIGIN      
        1 tttttggaca ataaccaaaa acagcaattt taactcaaac ccttgtggca gatatattta
       61 tgtatataat taaaaacata tgaccaaaat tttacttgac caagacaaat gtattggttg
      121 caatacttgt cctttgattg attctgaagt ttttgaaatg gatactacca tctacaaagc
      181 taaagttaaa aatcaacccg gcaaggttac tgaatcagtt caaagcgcca ttgactcttg
      241 tcctgttggc gctatttctg ccgaagaata attattattt aaattaatat gaccaaaaaa
      301 atcaaaaaaa atctcaaacc aaaatcagaa ccggttgcta ccaagtcggt taccattcca
      361 ctagttggag ttattatttt ttctcttttg ttggcagttt cctcattctt tgctggagct
      421 gcttggctta aaaccaatgg tggcgctgtt gccgtcaaag ccaaaaatgt ttttgaagcc
      481 aagaaatcag ataagcccga attccagttt tatgttatga gtttttgtcc ttttggtaac
      541 gacgccgagg atgttattaa accagtcttt gatttgctag gcaaatcagc tgatatcaga
      601 cctcaataca ttttcagcaa ggttgaaggt agtctcaatg attattgtca aaaaaatgtt
      661 cctgatcctg ctaactgtca gatgtatgtc caaaacagtc agggacagct caaaagtgtc
      721 gaagattgta aggctcaaat taacaccatg ctcaaagagt gcactaacga aagccaatat
      781 ctcaaaattg gtaacaacta ttacggctct cttcatggtc ggatagaagc caatcaggat
      841 gttagagaaa tctgtgccta caaccaaagt gaagacaaaa ccaattggtg gaagtttgtt
      901 gagaatgtca acaaaaactg caccgacaaa aatgccgata cttgttggga agatcaggct
      961 aaacaagctg gtcttgatac caccaaaatt actgagtgtt tcaacaatca ggccggagag
     1021 ttaatcgaaa aggaaatcgc tcttactacc aaaaatagtg ttcaagggtc tccaactctg
     1081 tttgtcaacg ggatcgcttt cccacccgaa tccgcctata ctcaggataa tactggtact
     1141 cttaaaattg gcaataaagt ctttactcaa agccaatacc gctctcctga tgctatcaaa
     1201 gaggccatct gtgcttcttt caaccgaccc ccaaaagagt gtaaaaccac tcttgagacc
     1261 cccaagtctc ccgacggtca ggttcaagga gccaccgagg gcggctgtta attttttggt
     1321 taatccctaa accccgctcc ggcggggttt ttgtttgcta aaataaactt tatgaaaaaa
     1381 ctaaattccc accaaattag acgcagccgc ccctcaacag ctcaaaaaat gcagtctcag
     1441 atcaaacgcc accccctagt tttggtttta gatcatgttt tagatacgta taacattggt
     1501 tctttttttc gtttggccga cgctctggga gtccaaaaac tctatctctg tgg
//
